<?xml version="1.0" encoding="UTF-8"?>
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<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLJ3</accession>
    <name>A0ABQ5RLJ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000233</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051309">
        <property type="entry name" value="ABCF_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42855">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42855:SF1">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="134"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="461"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="689"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="689"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="720" mass="80850" checksum="41DFAB6DA1DD059C" modified="2025-10-08" version="1" fragment="single">DTCYHNRALFSQSRPISHLGLLTDGMRQSLGKREATSSGRSAPPICNWRGRHCFSASANRKLAVLRGWTPLEQDPCTSGRRSTVFVRISEVAAVQAPVVATDPAEQPLAPAVETLNDAETEKSRTGSSGISSGIRLENIAMTFKNQQVLKDCSWEVKKGERVGLVGVNGAGKTTQLQIIMGRLQPDSGEVIKARRNMRIAYLAQEFDVQPGRTVREEFYSVYETQLKIKRRQEELSLSLESVGDDMDRMQQILDELDKLNNQVVDLDVELLDKKIDQMMPELGFRTEDNDRLVASFSGGWQMRMCLGKLLLQEPDLLLLDEPTNHLDLDAIQWLEGYLKQQDVPMVIVSHDREFLDQLCTKIVETERGVSTTYPGNYTQYVASKNEKTALQWSAWEKQQKEIARQEEIMQRLAGGAQSGRASQAEKALERIRAEGLIEKPFVPKKKSFTFPHVEKMGQRVLTIEGLTHGYPGRPLFKNCNLEIAKGDRVAIIGPNGAGKSTLLRLIMSREAPQSGSVRLGEHNIVPNYFEQNQAEALDLDLTVLDTLVQASPDAKLNDLKALLGRMLFSGAAMEKKVRVLSGGEKARLALAKFMCTQGTLLVLDEPTNHLDIPSKEMLEEAIRTFQGAVIAVSHDRYFLRQIATRVLLVEGRKLQDYQGDYEYYLSKNEDEAAKMEEKEAKMKEIEKDNVKAKSKMTKAEKERLKKDKAKAFHAQAEAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLY7</accession>
    <name>A0ABQ5RLY7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000355</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol polyphosphate 5-phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056454">
        <property type="entry name" value="Beta-prop_IP5PC_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF300">
        <property type="entry name" value="TYPE II INOSITOL 1,4,5-TRISPHOSPHATE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23754">
        <property type="entry name" value="Beta-prop_IP5PC_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="3">
        <location>
            <begin position="1136"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="235"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="461"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="573"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1329"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="576"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1351"/>
            <end position="1366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1705" mass="178611" checksum="23BBBFC488C1B277" modified="2025-10-08" version="1">MQPQSPNVSTSWVSFDDDIGGPIFRAPAAPSFKANVGTPSAQTGWAVNFDSAGPSTLAPEPHVGQGTVIYPAATIPPSVEVAAAAEGLASVAPPAAPLSHLDTSTPVLTAASSTSPQVPIFPSFASLYTGAPAAITTANTSAPSATIHPSSSFTPSDSASTAAAGSGNFIQGRLPRPPISMSRGAGALPSAAAINPFNSNDMPPHVLGAASSNPFAASANPIGATTASHSLDPFADLSLRPSLPRPPPMAASRGPSSIDSPSQVLRPVATGITSPGALQVGQGPSGDVPAGSERGGALVTQAGLGPAKDFGLTAPAGAGLRRPPTASVAVHTQSPPASQPVLGVAGGPVPAIPPVASTAAPSVFPSFATLHEHQHQQPKQLSAVPSAQVVVPHLLDDIPAVGSPAIALTGSTTVMARPGVGVGPGAFRVAAGTPQPSATQQELGALSRQGHGAIDLDTFLPAAKSSPPQLQGQAGAPPPPPPPYSAAVNLPDAASSLNGRSQMDLPLGLPPPPPYTEALDMPDAQPSSLQPPLPPSYDEAVTFTAAVEPSLDRVARPLPRDDVSLRGAYAAFDNGDVSSSQGTTQSANLPHPCSSAPSSSLASTSFAFQSSTAKASSAPLPNQICVEVRLPPLKPKEAAWPKRMVAGMGMLFAAPGVDGVAALQWLLLPGAGPGSEKDEGSGPHFALPRDPRVGLMAQRNTEDADSAPAASVLLPPTSELQSTCCMLLDDISGSLWTGHKDGRVVRWRVEPGMVAVYEHHWRAHVRGKITSMTLSSWGDLWTASSGGTIRVWQYQAGLPASRPPTKLFECRRCRPTMGPPVRGNAPRPHSKARLLCLGPSGRVVWSAGRTGMALWGAYDGEFLGTLTPGLQQDSRMAGGSLPGGAVAAGAYAGDTGALYKDGVAQQQQLDINPQTGLEAGLINRPFQPRRAPDLGDDAEAGDPDLDLGQQVIKGLAGAAKLAFKLGKKIRQNFSETTGVGAASEAVGISETNGNGPGSRGKVVALLPGLDQSVYVAYHGGLLEKYTEWGRLLWSRDYGRRVELYSAALVGSLLWLGCGDGKIRTATAASGELCHAWKAHDFPVMGLAHDLGSTGRGSALVYSLSESGSVRGWPAAPPSEAQRLAWRDGLLPSLRPHKLAVLVATWNVNETRPSSQSLSKWLEPASRADIVSISLQEVEMGTSSVAWDAAVTLLSKSMLERGNQNAQWWATELAAALTATGRTWERVALRQMSGLVIVVFCRTELQAHVGEVSTANVACGVMGVGGNKGAVAVSMSVFRRRVMFVCSHFAAHQERVDDRNENYNKIVRMLHFDNTSKVAQQQQMLLRQQQQQMLQQAGEAGGDRAMEPDFDGTATSSTIASTGASASENEDDGHGPGLSDAALLVWAGDFNYRINGTYAEVREWSCSLALRRLFKLDQCRIEMEKGNVFRGLREPLPVGHPVFVPTYKFDKNEPHRFIPPVEPGGRGRLNLPYDTSEKQRVPAWTDRVFFRGSRPGSLDVAAEEVTASIDRASDYNCVLDINDSDHKPVFALLQVQLPGYAQEQKRRHSLASALAVHQAVISSQGLGSSVQQPQPVQVSVSRLDLRSNCTSTLELFNCSTVAVIVQVMTERRHGASSGRHALAQLPCWLEVSPITFYLAPRLMEGDIGGDSSQGRARVSLRVLAGESLRPSEPLQLRFVFRPLWGSETLAAALGPVVSLTFGNSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPJ2</accession>
    <name>A0ABQ5RPJ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Signal peptide peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_001072</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A22B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF73">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="601"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="625"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="452"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="522"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="534"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="685" mass="70917" checksum="C3477D06B17CD931" modified="2025-10-08" version="1">MLQPLTHSTCRNRCFPTFSDLAMASPVMIGRKAYSFSRGTTIARPNQGSEVLRMFSSIKSSLKDGASSNKDVGTFIAVAIGNEQPIVSAVAEAAAVNVPNIVSGGKGVKDHSWRWEESDDAVRAYSSLVILLALGNLPVLHATKLADLPYFVGLALVTIYIGAHRGLTTRQRQQISIKEGILAPVLASVSLFGFYLLIKYLPDFNVKAFLNAYFWMLGTFAIGGAAFPVLRKVGGQWGELSVKFDLPDGWLLDENGASITRAELAPTDILAVVLALGLSTAELASGHTSFTLNNFVATLVATDILQLIGPRSFRTAGLLLLGLLLYDVFWVFGSPKVVGDNVMLAVATSDMISGPTRILFPRIPGGGSTAEAAAAAFPFSLLGLGDIAIPGLLACLALRYDASRSTDMRARAVAAAEAISSALAALEPGATSRQIADATAEAAEVAYDRVADQEARQRTQSLDGTNADGGGSSGEDGSTAATAAAAANLGASILRGVSAAASGGAGGSAALGSARSNLGLSASGRAVPGSNPPSDTETVSSTITTSTSQSTKMPLTSGQNDCASTAAATVLPAVPAPGEPPSAPELPRMAVSEAVLYQRTYFTPVMFAYVLGLVGAFIANDITGLGQPALLYIVPATLGAVVLVGVQRGELRRLWSFTDVPSYGLSVKEESKSRHSTSGADGHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPJ9</accession>
    <name>A0ABQ5RPJ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Programmed cell death protein 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001361</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007320">
        <property type="entry name" value="PDCD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12298">
        <property type="entry name" value="PCDC2 PROGRAMMED CELL DEATH PROTEIN 2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12298:SF4">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04194">
        <property type="entry name" value="PDCD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Programmed cell death protein 2 C-terminal" evidence="2">
        <location>
            <begin position="338"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="449" mass="48640" checksum="90DE523BFC0DBAFE" modified="2025-10-08" version="1">MPSVDAARLVEVAPEDEDEPEEILWNLGFVEEPEKPQLLLRHRFPSKVGGRPAWLDPVRLPSLEMLTCRASGRLLDFLLQVYAPVDANPVEGFHRCLFLFISPEGSRLGEPGAVRLFRCQLPRHNPYYSSEPPSNKDKYPQLLKPSDADASRERDPWRSVDAERLLAAVSTSRSQPSVKRRKEAAATAAPADAATTAATELAGAVLGDGDRNGAASTTAAAAPLPRTGVRGPGGLYSELELVVEPEDDYMDEEGEEEGRMQARRPGPVAGGPGEPRGGEEGRGDGGAVEGGDGGVEAEVRRALQQYRARVAEEGELDDEELPPDVMEAVEDATAPDQRCFVDFQARVSWAPDQVIRYCFQDGAQPLWPAPHGRPGPGDIPPCPCCGAPRKMEFQVMPQLLHYLQLDEDDPACPDWSTLVAYTCAASCSPPAGAECAYLEEVVWVQSPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQA8</accession>
    <name>A0ABQ5RQA8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001279</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003882">
        <property type="entry name" value="Pistil_extensin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF8">
        <property type="entry name" value="SD21168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01218">
        <property type="entry name" value="PSTLEXTENSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="450" mass="47835" checksum="AE0376EA4E90D0AC" modified="2025-10-08" version="1" fragment="single">PSPPPPSPPSPPPPSPAPPRPPLVMEFTLAFTYRTNLDVGRWYAQQDSTCLFVRIATRSASCSSQGLYFADYGVASKFKLVLTQGPSISAEYFEKEVNELTADPVAYLGQSYLNLYNIETMTAYWPSPPPSPPPSPLPPRPPSPPPRPPSPPLPPSPPPPPPSPPPPPPSPPSPPTPPPRPPPPPPPPPPRPPFAPPRPQPPLPPSPDPPLPPSPPPPSPPPPVPPPPSPPPPDPPPPSPPPPVPPPPSPPPPSPPPPATPPPRPPPPPSPSPPSPTPPPPNPPPLRSPPSPPPPRPNPNPNPSSPPPPNPPPPRPPERRGKTGTCYKYVVWCMNGFRELYDLVLDPYELTNRIPTAPSALLDRLDALMTAVGYCRGGDSCSNPYQVLHPDGSVNSFEDAMDPQYDVFYAGLRKFSFSKCSTGYFPGSESSWLMGDAKPSPPPRRKGGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQC7</accession>
    <name>A0ABQ5RQC7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Xrn1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001296</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-3' exonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF7">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="673"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="877"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="994"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="704"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="720"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="886"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1007"/>
            <end position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1033" mass="109101" checksum="A36782B977E5CBEF" modified="2025-10-08" version="1">MSSKRFSHVMFELSDGTVKGEGEVKILGRMARPWFSEDPNDTHVILGDDADLMLMALTSYKDNLYVANTGLNNGKLDKKLKVFSVNLLHEAWATTFLRSTIVAGLVPPQLALCQAKLDLTLIALLAKGNDYLPAMKGAALEGSDNRYGLWPMYQTSAAMVGSVPGANGQKAKVRRISETGFLTSIDPVTGAPRVDAAALAKLLQLNGAKDNVLDEADGSWRGTKAATNPRHYLHGCMWLLQMYLTGQCPDYRFVYDGVAPSVHAVRATCEALVAEGQTHITLDTFGFADRLADVPLLPVACALALLPSKCYHLAAPCVRQLMQEPNEDKGLEYVMAAGGGGGGRLPRFGATLYYDLYGDCETCREFMAEASETQKLYIEAKRAWDIAQKRVNDAEEGVAPLCEAPMDELLKEVHGAESKVQELKSKMRSTDKQRKKHISAAHPYRRFPVEELEAVTEAALAAGPVTPAERCATQFGRPLVFRSFARKPEAAAFIRAQSVVLSRPPQPGPQLSNRTYGTHAGYMQKDVMQKDVMQKDVMQKDVMRPGNADYEAIFRPPEPPFKRQGPSYLAAIVRQEVLNDEYGSPAPVQLRVLPHSPNEVVKYFIPEQKPELSQPEQRQDFGGKWTKANVVTAAAALGTEAETPAEGVGIAVAEVTTPAAVAAAAAAALAVMPPGNGSHKRSWLTSQEPSMGKAMKRKEEEGESGAAMAASAADPSSSRGAKRPGRKPKSSTAAGHMPAANNQDRMDGCAEDEPSSASSGLRREGATGTSKAIACVVAGVAAAAAASAAMAVAETGMHVASTAHSDLPSSGSGAAGATSAIVTPLAASATATKAAAVHATAMMHGPPPMPSINLLRIASGGGRGAYGAGALKPEAHGASAPVHATPSFSGEQLDAQPSTYGRGHVQPAATTYYNYDYSEAAPPPPRSRLLAPVPLSSGYMEQPPPDYAFPTAQQWCPSNAHWRSRRSAHHAGTPTAYGGVGHSVAPAVYRLPPPPHRPVSPPATVSAAAAAAAAAAAAGHNRMGGASAGPRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRR6</accession>
    <name>A0ABQ5RRR6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002199</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050817">
        <property type="entry name" value="DjlA_DnaK_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24074">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DJLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="8"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="230" mass="25175" checksum="F587A77341EF9B59" modified="2025-10-08" version="1">MATVELIAAYDKLGVMSTASKAEVKEAFVRLCKQCHPDKVPVAQRAVAEARFRDIKEAYDTILRGQAGYNVPPPGSGPNAKYARAYYRAHGMGMDFGERGPIRCGGPFGGFATEWDFYRAMFRTTRNNPFVLIAAGLLAIPAISFFVSVTNGDTAWIQQFRDQGLHMFGHESLKANGRDAVGINPFAIRRLEDIHDSYIYKNEKYAHLRKPMSAATGSVASDSGADSVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRS8</accession>
    <name>A0ABQ5RRS8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI60008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF260">
        <property type="entry name" value="FAD DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="55"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="481" mass="51868" checksum="3DDEE3780668C38B" modified="2025-10-08" version="1">MQHTVTSRVGVPTPVPKPTISYWIKAFPGAMNPIMSRPAPSTCGQPRPSLPQAADVVIIGGGIAGVSTAYHLRKTRPDLQVVLMEARQLSHGATGRNGGLLWPALNAPWVKTADRYGTTEASRMMAFELQCLSEVTQFIKSNGLEEEVRYAPFGDGGFYVYETQQEMEAELEELKLMQQFGIHTDAEPLSHRETSTLFGTDAFVGAVRQPGVSRVWAARLVHALARMAAAAGPTEGLVVAENTRVAAVTQSSYTEPLDKASAPLLKVTTETGESLLARRVVHCTNAYGSSLLPELRGALVPVRNHVAATNAPGAAAAIPAPVFPMDAAFFARGGYVYWSRREDGRVVVGGFRDVVPDLEWGVYDDASLRDRIRQELHAYLPEHFPRAFGGGLNPEVEWSGILGFTRDRFPFVGPMPGRHGQFVAAGFSGHGMTRTFSCGRVLADMVSGLPYDPNFPATWLPAAERFGPATPTVLGPDAAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS53</accession>
    <name>A0ABQ5RS53_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="216"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="142"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="216"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="278" mass="30948" checksum="ADE717CB887C4A3B" modified="2025-10-08" version="1">MAQQRRMPAHGPTAPVLGEGTDNSSITAEDVCLRDQRAAGLRFMKAFGVDVANDELADALSEATKAEVEYGGVPLGNGATLQQLQSHVQQLLQGQGGLAHSFQLMQQQITLVEQQSQQQITLIEQQSQQQMRLLEQQSQQQITLIEQQSEQQMRLLEQLMQQQMELQRQMGLLQRQTGLLQRQTGLLQGQMGLLQGQMGLLQQQWKLQQQQPGISSQQQQVSQQEQGIQQPPKEQQQGVLLPLPLPLPLPLPLPLPLPQQQQGIQQPPSSSSSNRDNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSA7</accession>
    <name>A0ABQ5RSA7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14873">
        <property type="entry name" value="OS06G0694100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14873:SF1">
        <property type="entry name" value="OS06G0694100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="677" mass="72029" checksum="C2BFC24EFDB00557" modified="2025-10-08" version="1">MYLPFTNVEAFGNPPLSPGISELLADFAPGDTDDASCKMADDVQDKHLQPIQPESRTSYVQYTLNDISTLPTGPQQHQASKQPMAGLDSLAQPSNFLPPLELMDWSNHQPFYSTIVSADAERTEPTFVSGGLASQPGILQQRHGPVSAAAAGFRRGRKPMPRLPDLQQRLDTLMEQFQRLNDENTFLKGKLKFLESVLPYRDSHIGFLAAAKNGQIPRSHVQYLQKGGPPEVAAATALPPAPTAIAPAVIMTVTAPTLAATTSSGSFSSETSLSPPAVAVAGSNHRITSVAGLVAAAVPPLPSTVSGAVPQAIGSEAEIRAASTRFVTKQVPLPCLHSRHSRLAEVYAEPNATAATATVTEASVVAMAATTGGYASPSAAAVARAGIMPDDVASRAAAAGSGRLTHGSTCGAMGHTSASGSWSAAPPACLPEAAAVLALCPRPDGEVPEIIPAAMEELKRVSARDFQVLYKHCVMQLSVLGTAAEVHGPSSPQHVRLERFLGRTFVYMDQINLLSPNCFVQSMYVNVETGEPERPSDDFWRTLGLSLKLTPEQLEEVRMVMVMHEMSVAPVARERLKLASELSASISAAVCQPPVSSGDAIRSLTEVDEVTERLRRNVIKEYQAQVDVSDFLCMSVLTPIQLARIMAASYPYIPDVVAVLHACDPAGSNTQRIRKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTF2</accession>
    <name>A0ABQ5RTF2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Selenoprotein F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_002756</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the selenoprotein M/F family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.50">
        <property type="entry name" value="Sep15/SelM thioredoxin-like domain, active-site redox motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038219">
        <property type="entry name" value="Sep15/SelM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039992">
        <property type="entry name" value="Sep15_SelM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014912">
        <property type="entry name" value="Sep15_SelM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13077">
        <property type="entry name" value="SELENOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13077:SF6">
        <property type="entry name" value="SELENOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08806">
        <property type="entry name" value="Sep15_SelM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047245143" description="Selenoprotein F" evidence="7">
        <location>
            <begin position="19"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Selenoprotein F/M" evidence="8">
        <location>
            <begin position="73"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="151" mass="16550" checksum="3E2EE5C6470DEB96" modified="2025-10-08" version="1">MIAFIPLLILSLTTSVLGVHPTCDELGFTGEQICSDCDVLAQYVKDEELVAECKACCAKDQVSVKYTKAELLVDQWRISALQQIKDFIDKRSSSYAPQLKVTYSSNAAPRLRLSGPSGTETIRVDNWRASSIAEFLDERLDRGGSSKGSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUA7</accession>
    <name>A0ABQ5RUA7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="152" mass="15816" checksum="33B6686BB7819AF5" modified="2025-10-08" version="1" fragment="single">RVGSFASTSPSSPPARPSPTQPLIGGDPRALVGTSLTAAAKALGYRDVTCTLLVKEDAVTHESVSSQSSIDACWLGMSSGDGTSDSQDASSGRPGCDGAASEIRDSNRPRHPTYHELRCGLLLRVLNRRRMNAADPVVGGGQGLVPMTRVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUI5</accession>
    <name>A0ABQ5RUI5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamate-5-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="2">1.2.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003074</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="194"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="631"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="638"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="661" mass="69077" checksum="09826778358ED3F6" modified="2025-10-08" version="1">MGCILVDRFGKPIAFREGVAAVRKITVQVAISSVTKQDGNGLSLALLGTVADQLQLLASLGYELQLVLSGDKAACEKAAHAVLSLLKLKEPVVDVLIEQTQDEAVLATRAAKAFGSGLLVYLSELRGVETAEGRVARVYQPATIGAGAAPPAPIVSAAWAAASSTDGCPVVLAALNESDVLLRLAAGQDVGTLVDPVAAEAASGSKAAAAASSARDMAVRARAASRKLQAMSSQERSAALLRVADALLAAQEDILKANALDVSEATGRITDSLLQRLVLKPAKIAQLAEGIRAIAAQEEPLGKLLRKVEVAEGLLLDKVTVPIGVLLVIFEARPDALPQIASLALRSGNGLLLKGGKEATHSNAALHKVIVDAIGPDLGPDLIALVTSREEIESLLALDDVVDLVIPRGSNALVSHIKRNTRIPVLGHADGICHLYVDEAADLDTAIRILLDAKTDYPAACNAVEKVLVHKSWTDKGGLKVVQEALQKAGVKVHVGDSLKKILPDLPIAPSARHEYSALAVTLELVDSMEAAIDHIHKYGSAHTDCIVTNDGARAEAFLRGVDSACVFHNASTRFADGFRFGLGAEVGISTSRIHARGPVGVEGLLTTKWVLRGDGHIVAKDTGVQFTHRTLTGDADSGTSTEVQQRGRQKHGGGPRCAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUY1</accession>
    <name>A0ABQ5RUY1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="857"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="965"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="693"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="744"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="797"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="857"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="880"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="968"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="986" mass="102555" checksum="54DBC883BBACC3F9" modified="2025-10-08" version="1" fragment="single">MEVFFLNSMQTCRWPIFKGHSQRTLDVERAYRRRRPCLSLFECPSRSPACAVGGNVPSGRRSTFIAAAAPTTSGTSRTETTPNGAPPSSPSSPSSLLEDPTHSHPQQPQPRGSKPRFRLLPEDLNDRQLPEDNPSLDDIVLDEQYYREMGLTEEDVQEALWQLDATEVDPEGLNLSPGAISMAQLSTDVAAAAAAVVPEPRVPLQSRAPGTFTRVMDEQSGRRGQLQQPTHGSINGKGAGKAPDTHSEDEEEEHCDETDGTNETKLLSPFGDDFGLDLSAAMTADDGDCDDNEVYGPEAVVLAGFRPEEHGLVRALLDTAGGHVVKVLPVTESMLYGTVDEAVHLAEQDWAAPRAPDGPRGGSWGSQRTVLFAGLGLSAQLTFLELLESSGFPAVCATAARCDLAGRRLGEVLAEAVADQRSQTNRISWKEGGEPWRAAAAATGHVADGRGGLAPLEELIGHKARDRLRQVDDNNDHDDNGATPVYDKDEDDAGGGGRGGAVPTLNSVGREAAASAEAEWEAWRVARNAAAAAGWGIETDDGVGRVSDKDPDPDPDSDLAAVPTLADMGVNLEQLREQVLRQLSEMDPETLDELLVDSPYGYAAEEALAQWRRVQADDGGQDRPVPAVPTVAGGPAAAAAAAAGRGLTADETGQLAEWQRQRWQKVKESLETAAAAAGQLVMHAESSLLQTEPPSPSPLPPPSTPPSPPPPQQQQQFQPVAPSIPFPEPVGTHGSQPAGMIEDATAAASAAAFSAPAHNAVSRTASGTSMEGPVLHQQQSPPRPPPPLHQQPVRQGPAPPAPTPTPAPAPTLDVLYPLNESPGPVISELNDLLGLDDASVVSELDDLLGKLTPATSTAAAAVTGVPPSLTSPLSAATPLPSLPFKPPPYPESGSGFGPGKTEGFGTSLAFVASGSISGGGGGGGGGVLQQVQEAARSGTAPEAIQKMLDERYGVTSAPLPAGAGPEQIAAASPGPPISAVTAAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV87</accession>
    <name>A0ABQ5RV87_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="6">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003174</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 6">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the NadC/ModD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="8">
        <location>
            <begin position="36"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="7">
        <location>
            <begin position="123"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="336" mass="35243" checksum="A60835E1C7710EBE" modified="2025-10-08" version="1">MASKSHITIPCPSHPTVSVAKVIKAALEEDAGNYGDITTLATIPESTQAVATFTAKSDGVLAGLGVADDVLAAVDPSVQVEWRARDGDRVIPGQTLGVLRGSARSILLAERVMLNFMQRMSGIATATAAMVASLEGLSTKVLETRKTAPGLRLVDKWAVLIGGGTNHRMGLYDMMMIKDNHIAAAGGIRAAVQRAEDYIRDQGLGDWMSIEVETSTLAEVDEVVEILRATRAAAHASKGADAGVTEPATTTIAPHLRRVMLDNMARRDPSKEGGVDVTLLAEAAGRIGDLAETEASGNVTLSSIRKIATTGVTYVSVGALTHSVTALDISLNIQTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVH7</accession>
    <name>A0ABQ5RVH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004061</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-prolyl-[collagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:18945"/>
            <dbReference type="Rhea" id="RHEA-COMP:11676"/>
            <dbReference type="Rhea" id="RHEA-COMP:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF238">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="8">
        <location>
            <begin position="64"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="197" mass="21521" checksum="4418B659A34BCFCA" modified="2025-10-08" version="1" fragment="single">AISEPRLIRSRVSTGAETPSRVSRSTFFTGDSARLPEVVGVEARIQQLLERPEVTGGTQPTLVKSEALQVVSYDVGGFYSEHYDNKAGGVISRAATIIVYLEDTPKGGSTFFPKSTGLPSLDKLCEVMPNLSAIPQLTLQLHQMNVPRPGLRIFPAKGRAVIFWSCLVDGTEDITSLHSAEAVRAGSKWICTRWFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVR0</accession>
    <name>A0ABQ5RVR0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004188</name>
        <name evidence="8" type="ORF">VaNZ11_004190</name>
        <name evidence="9" type="ORF">VaNZ11_004192</name>
        <name evidence="10" type="ORF">VaNZ11_004194</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="8 11" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="106" mass="11595" checksum="B89B6DBDEFBC5680" modified="2025-10-08" version="1" fragment="single">PYSENATPVYLTGEFPGDYGWDTAGLSADPETFKRYRELELIHARWAMLGALGCVTPELLSKNGVQFGEAVWFKAGAQIFQEGGLDYLGNPSLVHAQNIVATLAFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWD5</accession>
    <name>A0ABQ5RWD5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33874:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33874">
        <property type="entry name" value="RING FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="199" mass="22797" checksum="DAA3A0FC14F731F8" modified="2025-10-08" version="1">MEARSEAAQELYTELEEEVYHELEHFVADPAFCAALEKPTRDSVLARWTGWGKSQRQPIKVMDTEGMKFSERIVDDIELVPDEPTASSSGVASDNQQDSFVVIDKKDAVESMAYYIAACISDLPEAQTLTPKQLQTALLDALRTLKRSRFQRVCTWGRRMYRWSTYTYSAVQMYQNPWIMRALLAALWAGSRMSIRLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWH7</accession>
    <name>A0ABQ5RWH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PsbP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002683">
        <property type="entry name" value="PsbP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407:SF18">
        <property type="entry name" value="PSBP DOMAIN-CONTAINING PROTEIN 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01789">
        <property type="entry name" value="PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PsbP C-terminal" evidence="1">
        <location>
            <begin position="107"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="268" mass="29226" checksum="D76321C23B758E0D" modified="2025-10-08" version="1">MQVSVKITLGCGSLRLQPPGRCHKRQVAAIPVCNAAPAEHFQDELSTRRHLLLSSALVGFSLIAASRTKAAANKEVGSYLPAYGTEDFVLFVPSASKTPALRAGTVDNTSPYRFALPPNFVEQKVANIQSGNYCQPRCDEPWTEVIFEGAQGGRVELIVSPLQKLTSKKNVKVEDLGTPDQLLERVGNYITGTYMDEDALVASNSRTQDGLTYYLYELNAPYAKTGAHSYTACTVKGDLAFLFVASATDKQWGKLEGSLRRVVESFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWI8</accession>
    <name>A0ABQ5RWI8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004011</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010402">
        <property type="entry name" value="CCT_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31717">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31717:SF45">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51017">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CCT" evidence="6">
        <location>
            <begin position="657"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="233"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="313"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="632"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="707"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1003"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="244"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="370"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="639"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="722"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1067"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1088"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1104" mass="112122" checksum="670D1F449E4FC39F" modified="2025-10-08" version="1" fragment="single">MLIGIGTSTGSVGLPAVGLLAPSPRQSCDGTSMLASGNFSQPHQQQSFHAAVATPVSTSNGAGPAMGAFSLPYSGCVTFMPSIRADKGPPNPLYEAALLSAAGMVRPWRPGLLPAGLPVNAGGLQLPAGLMAAAAAGPLPLPLPLLGPIQTLASAAWAQAAAAQGSGPYNGFTAGSGMTSQAPGASQQQQMQASEEVAGAGQYYGSGGGSGGRTHATTGVAYATSPLVAFVQAHQTQQHQTNQSKRVPTVQSQQPQTSGLSATQQQQPPNIKQEHSTSSAKATSSHQGPVVQGQNQQYPHYTQPAQLANRMSTNGHALAPPGPMPAPVPAPGPGQGLLLNGGPFASGPTRDCGVANNSGSNAVQQPAPPSSQARPLSQQQQQQSFPQPPSHQVRPQQLSQHAQSLAHGLSQQQHQQQLHISGPGLSLPGQGFQQPSLHPLWQQQQQQQQQQQQDPTSEQSRGGLGFLSNSSNTSALLLFNQQQQQQLQQLQQLLLQSAGQGPRNQQQLLMQLAQQQQQHLMPQSALLPPAAGPGVSPDAFGSLFVGPMAQWLMMNISHAASTGNGSTTTANAGSGTALPFAMQLPPAAAAAAAAAAAVAGLTNLPASSALAASQGALPASVVAAAASAVMAGPAPEASKVSRERDPRDRELAVHAERDEALTRYRQKRKTRHFEKTIRYASRQILSHKRPRIKGRFVKTQPCQAAIIGMSPATTHEPRTSEDPEAGDEADDEQEAELEEVEEDEPLGAPKPLREATGPIMELDGPAMAEGPQEAVAEGGLGMELGTAGLVEACDDGQGCRNSCDTMEVDVSGGEAAYGVPQDSPCGGACGGCGCSNGGGIGGGGAAGAAGAGGCDRCGPGALCTAAAAGAARGESVGGSNGKASSQNATAAGAGTGFTIAGHSSALALAGCAHAGGHQLHHHPHHHYAGGRHSYTHHHIGVGHLNAMGQLGTHMGQLQHGMLGMGSGERLAPAATLAVGEDGPALGSGGTEGLRMLSDKDMQLSGPMPAHAQAALSGSAHAQGQRGISRDTQAGGAEEEEEAVGPSHSVNSRLSAGSVHTPGRRRALAGATGAVAAATYEELGPRGGVHSHTHMHPQPHAHTHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWN1</accession>
    <name>A0ABQ5RWN1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF135">
        <property type="entry name" value="CSC1_OSCA1-LIKE 7TM REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="517"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="570"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="641"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="661" mass="74201" checksum="77D36812BD2D5171" modified="2025-10-08" version="1">MRFQLYRDTQQVGEHFSRYGEVMDVVLVTDRLGATLKHCRRAARLQQRQAMVLDAFKVRQSATYTAMVDKFDKQLTDIAEEVRELAESKQKVKAAFITFNSENERKTCTSYCPSGWLASLFQRRKDRFLLQHRFWVHQAAAPDDYQFEDLAVSEWQLFAREVAINVVMLLLVLLCASAIAKLTDISNQESYTVNFYKDRLVADSAAAMVLYTNTNSSITSTHAGTEANASALLGSELYSQLEGFCSRQLQDSCMMRLQSEYFGLVRMDYGAGLTWVNATSRLLFERALQEELECSTAASMQNDMCQLQGCLSCICLGIALGGPVTSEMGLGSSRNPSNSDVESTCSPYINQLDVRSWGIRLSISAVVAVLNSFIKWLLSVLVVQGRHWTHTARERSYALQCFVAMLFNTVVVLLVTNCEPLAKLARESQNNNWTRFFVKYGSYDDFTPGWYENVGLSILILMIINISGPLLNLLLEEALLISRRCCVLYCIAWPLQSDYDVAWSRPRFTLAQRIADLLLNVSLALLFGSGMPLLYLVLALYVIAAELADRWALTRLCRGAPRYNTGLMKVVMALLPWMAVAHCAFGLWMHTYFRVVVPGNSNGIEEVSHLTEAVNNAALKSSSSNLSVLQRSSVWHRITQPNGLSLLCVMAGLVLWLVIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXG1</accession>
    <name>A0ABQ5RXG1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004714</name>
        <name evidence="3" type="ORF">VaNZ11_004720</name>
        <name evidence="4" type="ORF">VaNZ11_004721</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="2 5" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="136" mass="15428" checksum="BFD9D650AFBE55E2" modified="2025-10-08" version="1" fragment="single">EEIGRLKHLVTCTEKRAADATADYDGAMRMVARLKLQLSEVWEGKASAWSIRAEATAENVELRAQLVEAKVTIARERSLARVDRREEIRCLKVELREVNESWSRRCEELKDALWRRESQAAGRTGTAACSRPTSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYS8</accession>
    <name>A0ABQ5RYS8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="251"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="332"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="875"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="346"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="878"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="904" mass="95298" checksum="06059760DC6CCBF6" modified="2025-10-08" version="1">MSTTMNDVFSGLFGCFKRCNGYTKKPIADIARTNDSRNSGHTVSISKQQQPDNQYGINSNSCESVAAGSITVQENVVHRRHWPRADTTASSSSTSTPTQIDFKDPADRELPSIISVGSGPDPRSSCCSHGSVPSPSLPAIHTLPCAGRSADTQFRTPTPTMMLSLRNLHMALAPMDCATKQQQQLLQLQLQQHSPFTNDSPSGDIRPSSIDFTGGIGFGELTTSDSSGNISMSGLTPQLRFVPASQLGEEVQDLQLLGRGGYGMVMTGNWKQEGRVAVKVLMSDEDKWVESCYKEAVLSRLLAHPNILQTYNFQVAVLTHADVARAKVSWEQQQQFQNQPHHHRTSQQSHLEDNPSSITKMPYAADTRADSFELLPPLCPTGGSYAYDRLGATSSSIDLWGVLFMLGAKPGQFVTLIIMEYAEVGTLQRAIAAGAFRESSRMSKWAALRSLLVTAKEIAGGMCLLHSYKIIHGDLSATNVMLRASRIDKRGFVAKVADFGLSKVTLNGVVRTEDWAGQAQYLAPERLDYEVRLASDVFAFGVLLYELATGRRAFGEYAPAQILAGRLAGDLTLRWPADVSPGVRALAERCMQQDPEVRPAFREVVDELRRQDLEAKAQHRQQAVRRRITADGLAAATAAAPPTAVAAATAMAVAVAGAAGATGAVGRMPSLLGPYRFTPAVYCTGGGSAVPPAAGSRPETTSLACTPQCVNGCSPSAALPYQSSLLRHSFTTGSTGLVIAPTGSAAAAAAATASGLGRAVTSTSVVVPASVVMSSPPGAEADTLSEFTAMGLRSVSSRTLVGVCPSEECSGRLCTSVPYTVPHFLPCPLQLIPSGDVCVYDEFPDKSRDIAFTTASLGCGVAGAAGATANPKGFTPAGRGGGGGGGGGGGGGPMFPRNTLFDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYU6</accession>
    <name>A0ABQ5RYU6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">procollagen-lysine 5-dioxygenase</fullName>
            <ecNumber evidence="2">1.14.11.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_005305</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-lysyl-[collagen] + 2-oxoglutarate + O2 = (5R)-5-hydroxy-L-lysyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:16569"/>
            <dbReference type="Rhea" id="RHEA-COMP:12751"/>
            <dbReference type="Rhea" id="RHEA-COMP:12752"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:133442"/>
            <dbReference type="EC" id="1.14.11.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.4" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021067">
        <property type="entry name" value="Glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34496:SF9">
        <property type="entry name" value="[SKP1-PROTEIN]-HYDROXYPROLINE N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34496">
        <property type="entry name" value="GLCNAC TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11397">
        <property type="entry name" value="GlcNAc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="9">
        <location>
            <begin position="169"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="286"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="691"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="694"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="884" mass="93649" checksum="0A52E5A2F3FE6736" modified="2025-10-08" version="1">MDIAEKINLLPDCVRRQIFRAVCADSSGPCPSAPSGALDVRKTQNNINNKLQKGKSQARIPTLPACHQALPVVRMVAGACIVDNFITQDHTKEALACARQALAEGQQHAAIGGALPLTSDSRHVDTAARGDIMRWLHAEREATAGRGPLAALLGQMETLRQQLEQQGYSVAGCTTYQLACYPGNGTRYVRHADASLSCPTRTLTAIVYLNEADWDTQVDGGCLALYNVPYSGPLFGCGLAAGEPATVVAPVGGRLVVFESHLFHEVLPAHRNRYAVTAWFHKPQPQPPMGMQDAAGAEPTAASLPLPPCPVSYNTEEPSLDASRGVSGPGESPPRMFADSVKAAERRMPAIATAGAEGTPGLQAAASAAVNVTAGDMAAADASTALNAAPAPAPIAVSSVTAEGDTCGQIDSVRVRAGAEPVTAAAVRASIFVSMAAYRDPEGRWTLHSLFSKAEHPERVRVGVVWQIDPVTEAGFVGVAGARTHPEWLRQISQIVVPHQDAKGPCTARAQAQTLWSGEEYVLQIDSHMRMVSGWDTLCIQQLAAAEAMSNSGKAVLSTYPLGYSGAGPAASSPDDTSAPATLLCAKGFEQDGFLRTVGRVLRKRPAAPLPTHFWAAGLTFTRSAWLREVPYCPYLPHLFFGEESYMLARMWTRGWDVFAPAVPLAFHQWERSARAHPCQRDMASEATIRGDHMPGQQQQQQPEQQPEQQGQQQGPEQGMEEGQGQVLWQGATDGAWMTGTLNSKVANEVITACSEAQGTDATIADVTIASIRKCSQAHVLRVLSGNVVATAAVLAGEPSGAHSLQKCGICHAEVLAQRSSSLGGGIRDREGGISGGWGLGQLWGLGTLRTLEQLAAASGVDYTAKTVSARAQWGGLSQDAFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZK7</accession>
    <name>A0ABQ5RZK7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncoupling protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">UCP2</name>
        <name evidence="11" type="ORF">VaNZ11_005748</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="31"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="134"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="227"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI62909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="315" mass="33342" checksum="E50F4AD60926746B" modified="2025-10-08" version="1">MSVEVLASSSPHKGVKSSYRNESQRKAKLWQELIISGISVGGATTCTNPFDVVKTRLQLQSRKTPGAPLPGLFQTAANIVRDEGVIGLWKGLPPAVSRGFLYGGMRLGLYGPSKDVLLVGVETVSGGRSPQGLADFVLKATAGIISGGVAAGLTSPTELVKTRMQAKGSHPQGTWDVVRQVVKVSGVKGLWRGAVPSMTRAALLTASQVATYDTVKREVMRQTGWGDCLSTYLAASSLTGLVTTTVTNPVDVVKTKMFIAGGGNQRAVALEILRNDGARGFFKGWSANYARLGPQTVITFLISELLRQQLGLDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZV1</accession>
    <name>A0ABQ5RZV1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005896</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="920"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="993"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1128"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1285"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="697"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1014"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1134"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1144"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1300"/>
            <end position="1313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1329" mass="133124" checksum="2F7F23B2E2D9993A" modified="2025-10-08" version="1">MTSSKHCLKTPRLRGSQRRHRGLASSFGLKQCAGSRILWYLLLYTNLAAGLHDALRAMPPCKPEHVDCNSHDASPKRSLGGEEDQSHNLLPHHDPTHQSRQPAFLPQSLAATSRALTIRSSLPPASLLDLKSVTSTSSGSSTVTDGDALRCGGSGGSVSSNSSSRSRSSGGSHRVVLEAPGGSATAPPDVFPQSGPVTSPVPAGGGSNGGGGGGTGRKQSTTIIIIAVIAGTTGLAAVVILGIVLVRLWRRWRRRQDSQILDSEATTGERTFDSISSVSLSDTVKELPEDSARFNPAATAAAAPPLADAKRDGLESTPFCPSSSKSLGSYPARPCSSMYGGESGTSGGATGVIVPGASSVMPFPPPAPTQISVSTPAVRASDSLRPSWRQYSVHSGGSDGGGGGGGGSDGGGNDSRGCHGGEKAETSLRPSWPGSVYRRGAFCWETGGRQASTGAHDDNFGGNGAVGTDAGGEPCGALIDGLPAAPGSSMGQCSRPTGGSMVSASGITAGGWYDLFGFEGAELAGVVIAGRPTTSGRVGGPAGLAARRRRRSSDSTQILRRSRGPDMGAEALPASLPRADSRGGDIGGSYGSGDGYHDGSGGGCRGSGSGSRSPVPLPQMMRPASDTSRYMYTDSAAAAARAAAAAAAAAAAPPLSPLSPRTHKPIEMSLPGSEPYGLNEEYPYDGVQPGDGCSAAADSSGTNPGDSSVVAPSHRDLLRVQSRRPAFSDRTRGILAAAVTATTTPPCNDGGDAAVQRSVLHDISTQVEPRRGGFIHGNSISPYIVIPPLHTSFIRTQNLTGSVTAGIGGGSSGPGSGGFAAWPPVASGQNSPCCSLGESRTALTGDVGGLRRSSGCNVAGRSGNSYPPAAAAADKPGDLPIYMTAQSMEAPPSPASASASASASASEWMGGVSCRSRSEVARRSASVNQHDTSRTNATAAAAAAAAAARSNHDSGGGGSGGGGGLLPAAALKATAAIESADLDPVYARYKIRRPPRVQTSNAGLNGGDGGDGSTPHSRSRAAQNGTGEREKVAATATRGQQQTGPHLLQLPQQLFNRTSAPSPSHHILSPLQPSLALEADEAMTAQSLGSSVSMRPPAALSMSVSRLTGAGYRTAPAAPINVEVKPSISPCSHNSTSGSADGSADGGADGGANGGDGGATGRRIAEELSPAFGPTASMRARQTVKRSSSCGSSDDGTQYGMGGLVDSGFAANAETGAARLTMMKRLAVGPPRPAWGLIHAGIGPFKRSIASVQNVRNRVDTGSSDSLTPAAVAAAAAAVAAAPSAAAARGPRSSVPGRMLLSGSSSAAGDLSAASSLASGPFLQLVAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZX7</accession>
    <name>A0ABQ5RZX7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Tryptophanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_005519</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_B">
        <property type="entry name" value="Trp_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024109">
        <property type="entry name" value="Trp-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050203">
        <property type="entry name" value="Trp-tRNA_synthetase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43766">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43766:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI62773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="439" mass="48223" checksum="928632216B56F676" modified="2025-10-08" version="1">MRNLGFSTSTNIPQGQSTYNKQQASMRGTAQLWQLGHRQLLRAPRPFKSSRNAQRVCSLETCCNSAAAVSISTAVEAQSSSSGAATTMEKVSQRKRILSGVQPTGRPTLGNYLGAMKNWVALQEQYDNFFCVVDLHAITVPHDPRELRASTTSMAATYIAAGIDPDRATIFVQSHVPAHAELAWLLSCVTPVGWLGRMIQFKEKSRKQGEDVRTGLMTYPVLMAADILLYQADLVPVGEDQKQHLELTRDIAEKVNLEFGGKGWKKRGGRGGRVFKVPEVFIPPAGARIMSLQDGTAKMSKSAENDNSRINLTDTPEIIASKIKRCKTDTVEGLEWGNPDRPEASNLLTIYSLCTGKSKDEVISECSSMRWGGFKPLLTDAIVEHLRPLQSKYAELMADKSYIDAVLAKGAETASMTADKTLADVRDAMGFFAPFRTRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Z5</accession>
    <name>A0ABQ5S0Z5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TFIIF beta subunit HTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_006410</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="8">
        <location>
            <begin position="218"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI63557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="290" mass="32523" checksum="220326AA4C00E9E9" modified="2025-10-08" version="1">MALDTRNADCAAWLIKVPYALASVWKAACEQSMAGQVDDSSEGPSFLGRMVTEPTVEDGNVSFLELPETMWAEGIPRRFKLQQMPLPPGDATNKMHVMSYEVPPSTAPGSDGGASGRRAAPLELSRPRVDGTIEQRMDMVPMVKDEKGEWTLDSEYLKLVRSRTEQAAEKVRIVGYYEDSVDNDLANLAQQKRVERDISARRKATVAAAKADNMKRPRLERDELEDLLFRLFERNTAWAFKALISETRQPHMHLKAVLEGIATQQKRGPNKDKYELKAEYRSKTKAPPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S128</accession>
    <name>A0ABQ5S128_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006591</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="589" mass="67060" checksum="62131A0035B7CAAD" modified="2025-10-08" version="1">MHADTPANRKLSLWNSHTSATGCGHCLLKGVSGEGGGMYFPGYAVDADVNCIFDGVIPDMENAPLSARCGDPRIMVSHEMHLKRAALAMKDPQWTGIVGCKGLSPIIRELDYCNYNDVFVVPVAHAALFGVIKRFWNTILGKLGRHVMDYAILGPQRKIMAQRASHIITTNDLNRPYRDIVKHRGTWTMEDWLHWLDSYSVYILHEEDSLQSSTPLMPVVQRVVDGITKSFNIKVMWNNLRTALLHYFRYDPSDFSADANNHAARLLKSFSIAAEHVFGIQFCTYNLHILVCRLREQENARGHVAFATEFWVERGMQMMKSIVKYRSPTVPELVLAGGYLASLWLTHLKAAHPETFRTFDEWVPGYRADNIQLNPSKRDAFLDEEDSEGSQLLGQGYIADAEQRSHILGGILRTEETEDFMARTNLLPSVLQDSDIYIFKRALKRRDEVVLSVDYARATKRQSYHFKCKYGPGGMVSESDPRVSIFIVEILFFALIRPRCDVAPVKVAVCNLHQAEVLRPKELYRVKDYSRRDATVNEVRQEILLNYFPKEVGMIDSKVIRCRVNNELSYFLTYVTFSQLPTNDGGEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S255</accession>
    <name>A0ABQ5S255_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34286">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34286:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="117" mass="13334" checksum="A1E244442CA3713F" modified="2025-10-08" version="1">MGGGHHHHPPAVPEPSYAKLLAPKSGLCPPDFFYNREIWYPHGGFYCDPKAWRRNTALTFGALGGLMYLLWQHSVSNEVRHMPPKGWIPSMIWNSNVPDPVDFRGRKLDRNGNLVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2B2</accession>
    <name>A0ABQ5S2B2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006588</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045874382" evidence="2">
        <location>
            <begin position="22"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="15649" checksum="F9FE54F9D342851B" modified="2025-10-08" version="1">MGKPSKAHIGLALGVLRYLVGIKELGLCFGGDGDLAMVSYSDFDWAGDPATRRSTIGYVFLRGGGAVSWNSQLQRNGGSFVSGGGVPGHGSCSTGGALASQAGRRLGFRFKCYRDSDGFPQADEHGEQPDNVGSFQAHRHAASPSMGARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2W4</accession>
    <name>A0ABQ5S2W4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007431</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="12908" checksum="20470D966F52138B" modified="2025-10-08" version="1" fragment="single">MDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTDKTPGHSIADATRAYVRDAKKYLQAVHGGDNDGIRCLFVGNTLEGAAKDWYRNWTNARGSYTFDELIAALLARFATEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3J0</accession>
    <name>A0ABQ5S3J0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007458</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="399" mass="41962" checksum="49BA2636FAFC5CA6" modified="2025-10-08" version="1">MSSEVAPSVWIPSRTMAFVVPRGLTGVAVAVLATALCILSALFIYIYHRRRVQRGRSRRPKGVPPMPTSAVDAELIVDDYWPGSRTSSRRQLFWLLDLVFPNTQRPSLFPDAQRGGPSFSIAIDRLWSPPAQQPEPAGDDLPDMPPYNDGQANGAGPRFLATTASGKSLQVVVKTAAVASRQQQRQHEQTIGLSQEALQTSWSCQATLEIGGGASEAGAGRHVRLQSLSDAELPGMRSLPQDSERPEVMARMIASSGGSGRRARGLRGASVNGAVSHMIPGPHSIAPAAALDGSDTDTDTSTDHGMAGGRANTVDQLHTHHDAACFRSSEGGQMLLVNSLAAGHAEVGSRTGSAVSNPLGRGPEMDPAVAWYSRALHRQVSAAAAAAQAAAGTAKVHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S442</accession>
    <name>A0ABQ5S442_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="135" mass="14842" checksum="18DC6B00F4D9EFFC" modified="2025-10-08" version="1" fragment="single">LLWRMMHEALPANHSVLPYDPDAFRTPVDLLPPKLQAALSWAWTHHFRPFAHDFGFLLESLPAAETVAGGDREVAATTARTPHVAASPPYTADGAYGNVDELSHYQSVLAQMVAFLRDNDMFACLATLLQTAVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S451</accession>
    <name>A0ABQ5S451_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_008030</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="97"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="372" mass="42180" checksum="69FABF2AFAD5839A" modified="2025-10-08" version="1">MENINDAKGAVQVQRRLEVASFSKRVAILFCMPLALISIIAILVEEFLRSPSKFFTRSQRREVQLPPSLESAVHEALDVGSGVRLHTVSFGRQPDKPLMLFLHGFPECWYSWRDLLVHFHTEYDVVAVDMRGFNLSSKPKTISAYSLNHLTADVAAVVRALGHSSCTLVGHDWGALVSWTAAGRYSGLVDRLVVLAGPHWWMYQRNLTIEQMARSYYFLWFLLPVLPELLLLHTDSAFLDDIWRGTVRPHGTLSATDVEVYKAAMQQPGAATASLNYYRALMYTYLGLMPMHPEVEAGVLRQLEMPVLVVWGEKDHALCRSNNRDIGLIAPRAEVHILPNCSHWIQSDQPRELRRVVEEWLAENPLPARKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4H5</accession>
    <name>A0ABQ5S4H5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="475" mass="50546" checksum="013E98486E9FFC61" modified="2025-10-08" version="1">MKGCPAYEVRHISVLHFGRFSSVRRGQLLFASTTTATIKTLVPTFCTTRQLTAPPTPTANLTTNIRTACSCPSDQHPASSFSESSSVQPSATPDSSPKVSTSPLAAAAPKAAARSPGPAGSAPKPAPSNTNFCQMLSRYRSVSVLALPGFYNNPAQGFEENREWCQECIRKAGSGEILRTAFRLWDGPLFGIAHQDGQFAALEEGPAGEAVTPVPEGLAKGLSLDLDFLVGLAELTRNLQYNAYSSESDYNVIPIILEPRLDASRRLAPAGEPQSSSHAAASGSGGGNGGGGDGGGGCWWSLEQALATPLSWEEVAASLGVPWNLAEVAVPPVRFDSKEAFLSHIVKKHVSHMDGGEFLDRIAAQNESRVNLPGSPAASAASRLLLQPEEIWRQIGLGTELPPPLPPQQQQEQQRNPETMLLAVREFADVVTFPVVLEVVVGSDELGWNPIPLLWLGRSRRSGAILGLMTAVVWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4K9</accession>
    <name>A0ABQ5S4K9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06174">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23525:SF1">
        <property type="entry name" value="NODULIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23525">
        <property type="entry name" value="TRANSPORTER, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="827"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="870"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="896"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="993"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="595"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="779"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1036"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1050"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1062" mass="109785" checksum="48045683E9FE5EB2" modified="2025-10-08" version="1">MVNYNVRITFAVRITDGIATGIWASSVLSTYINVLEGDDDRANERVGYAQAIQGTFLAAAAIPAGWLAIKHRRDSILKVLGLVAFAAIAITSAALVLDVPYKYSLLCCGLALWGVAQANAPVLDALFADSVPTGNRSKLYTWLHMSYIVAQGIGPGVGAAVFALNGNVWKLEVLQHVMLLGMALAIIPALLMLCFDDDMALGAESEGLLRVALLRPTNEEEGREEGVEGSGGSRRHDAYNSLAGSRSQSRTGAEADYRRPFLEGWCSDAERDYRNQVSGACSPARPVFPTIAAAAHCYNPPNVVPISSGGAYGSRNVGFAAVNKSTSSSAGMAVLTSPRVSDTQAATAAMHGAQPAVAPAQAQAPVLVPQILSHTLLLSPNTSPTIDLSGIATRVFNSPTPPSAAIAIGSSSRSSSASGGGGAGFLNAAIGGAAVAEAAVGLGELLQSGDRGEVPLGRCSAGGDGPVQGKDVRMGGAGASVTSAAVAERFHTVREAVDEAAVAAAEGPSSGADNVSSYESFCNMSIRVRVMPRVDFNSGGDGAAGMIALDVDDGGAGSSREPSLMADSVRSELSFHSLLTPGSFKSVQSRSFSRVGSSSSCGSGRGGGSSSGTGGGALAAVAAAASPPLALLPFLEEESTEEDDVVSEPDTPKLHPQTQQATLDHPPPLLYPVSSAPVAVPPAESPVITSDPPILCRDSSSSGLTASSHGSSSVGTTFLQPSLSTRNFHIISPSEQLPDTSAAAAAVAAVPAVAAVASGAAVVYPNGIHGHMAVGSGSGGDGGFGAREGSRWRPFEPPPAATASSPRELPPQLTAVSTSLDPKKFRFLCLNMAWIPGVLALTDCTMGLASGMTIKFFPIFFKDAVQLAPMYVNLMYCAIPLCLSAAAYLAQRESRLLGRVQTMVLNRGIGISLLYWIATHTNRWHDQRLMVPVYIARTSIMNSIYPLQKSILMDFVPKERRAAWNSLESVTSFGWSGSAALGGWLIHRFGFQTTFLITATMQCIGWGLSMMLLPIVPRKEAGLQATCQKAASASVTAGGTSGRAGRHNSPVAQSSPASSGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S590</accession>
    <name>A0ABQ5S590_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Dynein, 78 kDa intermediate chain, flagellar outer arm</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_007693</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF11">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="513"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI64492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="75909" checksum="CDFD3C0195418D61" modified="2025-10-08" version="1">MPGPITAKKPLDKGKGKKKALEPAQSQDYVPPPPPMPGDEAFAMPIREIVKPDNQLRLSEADLNEEVAKMLTANNPAAPKNIVRFNMKDKVYKLEPMVEQTVVHYATDGWLLHKSSDEAKRQLDMEKMEQEASARFQADIDRASHEHKEHGDVEPPDDSRQLRNQFNFSERAAQTLNYPLRDRETFTEPPPTATVSGVCTQWEIYDEYIKDLERQRLDEAAKIRGGKKHHLAAHHGGAASKQQEHVPTLQSPALLHSLGTLDRMVNQNMYEEVAMDFKYWDDASDAFRPGEGSLLPLWKFASEKSKRRQVTSICWNPLYDDMFAVGYGSYEFLKQASGLINIYSLKNPSHPEYTFHTESGVMCVHFHPEYANLLAVGCYDGTVLVYDVRLKKDEPIYQASVRTGKHNDPVWQVVWQVDEAQKALHFVSISSDGNVNLWTLTKSELIPECLMKLRVVKAGETSEEDPTASGPAGGCCMDFCKMLGQESIYLVGTEEGAIHRCSKAYSSQYLSTYVAHHLAVYAVSWNHIHPNMFLSSSADWTIKLWDVHDSKRSVMNFDLNDSIGDVAWAPYSATVFAAVTDDGRVHVFDLAQNKLLPLCSQKVVKKAKLTKLVFNPKQPIVLVGDDKGAVTSLKLSPNLRITSKPEKGQKFDDLEIAKLDAVVEIARKSDADVAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5B7</accession>
    <name>A0ABQ5S5B7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="348" mass="39006" checksum="70465DA61B8362D8" modified="2025-10-08" version="1" fragment="single">DKCFAAGFAQVADLQPRVTVDCFKKKWQKYATDYNAQRARYEKVKATHKEELACKPEDLEKKPWKKLWNKESRAAWRSFKYAQPDNPDAGPSERRIAMEDLNVTDRQQELINALVQRKQIESSKTSFTVPATPVPVGAGGTAQHTPGLGALELPKGTTKSGRNDEPPGTQDGRTSQRQKQPAHGEDALMMVLAANAARIHQKEKEIKAARRRQAKSSEPSKARNSSRNATNSSKAAAKKVTNNDDVRPSTKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRRKKVKSASEDVRCTRARRQRGSSGSKKKQELLQEEVSKDEKSEDEQDDATSAREEDDNEDSEIEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5C1</accession>
    <name>A0ABQ5S5C1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_008541</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056158">
        <property type="entry name" value="Beta-prop_IFT121_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056159">
        <property type="entry name" value="Beta-prop_IFT121_TULP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039857">
        <property type="entry name" value="Ift122/121"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057979">
        <property type="entry name" value="TPR_IFT121"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056157">
        <property type="entry name" value="TPR_IFT80_172_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057361">
        <property type="entry name" value="TPR_WDR35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017233">
        <property type="entry name" value="WDR35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056170">
        <property type="entry name" value="Znf_IFT121-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12764:SF5">
        <property type="entry name" value="LD29485P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12764">
        <property type="entry name" value="WD REPEAT DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23390">
        <property type="entry name" value="Beta-prop_WDR35_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24797">
        <property type="entry name" value="Beta-prop_WDR35_TULP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25768">
        <property type="entry name" value="TPR_IFT121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23387">
        <property type="entry name" value="TPR_IFT80_172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25170">
        <property type="entry name" value="TPR_WDR35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23145">
        <property type="entry name" value="Zf_2nd_IFT121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037536">
        <property type="entry name" value="WD_repeat_p35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="IFT121/TULP4 N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="IFT121 second beta-propeller" evidence="11">
        <location>
            <begin position="341"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="IFT80/172/WDR35 TPR" evidence="10">
        <location>
            <begin position="689"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="domain" description="IFT121-like TPR repeats" evidence="13">
        <location>
            <begin position="1025"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="domain" description="IFT121-like zinc finger" evidence="9">
        <location>
            <begin position="1158"/>
            <end position="1200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI65100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1227" mass="136755" checksum="A9ACF210AEC90E2D" modified="2025-10-08" version="1">MFIYLSKKIAIPNNVKLRCISWNTDQGWIACGGETGLLKVLRLDGTTSKDVKGKKEGGPPGGNLSLNQTLEGHNGSVMCVCWNNAFQKLTTSDEFGLIIVWMLHKGMWFEEMINNRNKSVVKDMKWTANGEKICIIYEDGAVIVGSVDGNRLWGKELGMELALVEWSPDGRLLLFATPQGDCHVYDGNGNGVAKVPLYCNEGYAGASKIIGVEWYDGVEGYAEPNCPVLAICLDNGRMQLMRYESDDNAVCIDTGIKPLKVKWNMNGTILAVAGFQMSSAVADGRELWMVQFYNHNGEHLRTLRVPGGGISGISWEGNGLRLALAVDSYVYFANVRPDYKWGYFCNTLVYAFNRPDRSEHCVMFWDTKNNDKYPKYVRKLVAIQAFGDFCVLATKGENQGEHILILCNAIGSPVDSKYIEVEPKYLAITNYHVIAANDEVVYVWQFRTSFSKVLSTDINAVKRKDVREKMFSVDDANPALSDRSPESFRNLTTQTTDPIACITACDSVLMIGRASGVVNRYSLPHLTLDAQHVLRCRPQMLSLNCNLTKMSIIDINGVLTFFDLTAKATGGGANTMGEHLAFERKDAWDMRWADDNPELFAMMEKTRMYIFRGLDPEEPVTSSAYLCSFHDLEITAAFLDDIMQQPDQPDLEFMVMYETRSLRDTRELLKSASVDDAYSFVDSNSHPRLWRNLAEHALEALDFTTADKAFVRCADYQGIQFVKHLGKLDDKAKQRAEVAVYFKRFDEAEQLYMRMDRPDLAIDMRMRLGDWFKVERLIRESSGDDSQLLNAHNKIGQYYSDRHKWGKAAQYYAQAKNSEMLVECFYALEDFVALGRLMDALPDGSQLLSNIGEKFQSVGLCNEGVTAFLKAGDTKRAIDCCVLLNQWDQGVQLAQTHNFPQIEQLLAKYANHLLEKDKVMDAIELYRKANHSMEAARLLQDLAKKCAAQKVHPMRVKKLYVLAALEIEKFKKRTLELSGPDKTAATLLGAGGGVGGTATMAATAAQTLAGLMTLESVVSGEAGVDSAWRGAEAYHFWLLAHRQLYAGNVDLAMRTALHLREYEDLLDPLEIYSFLALAAFYNQFFGQCSKAFIKLESMPSIPTDKRESFADLAMSIFLKHPPADPRALRETREKKGQGGATGSSLDALLEDLGGGREQVCVASGRIVRDGNVLRCRTCKHLSITHELRGATVCPLCHSSLPASAAAGPPARTISSKAAQMSHLYAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5D2</accession>
    <name>A0ABQ5S5D2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="251" mass="26927" checksum="2AE3BE55B8D47AF2" modified="2025-10-08" version="1">MVMTEGVEFRRKAVGGPDTTPDELLFPYQHRLVDREHLQVDISLTAGSPDSTPRWDAATIWNKALQVMVYAEKSVRRPKAGSLAVAATSSESFFRNGKAAGVDASPRDVMSLFPTEDSWISPKKNSYTLVGASCSRGSTTKRSRNGNEPSTSTLECASSICFDSPTNNKKSSSNDYNNDNNGGTPASFLSDVQNKLPMSMTINSAPEAGATSLGSSAMLGQVASASMAIMQKAMSAPMKRGILSKRKEKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5W9</accession>
    <name>A0ABQ5S5W9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="25948" checksum="8DC8D59B0417E4C7" modified="2025-10-08" version="1">MEISRGVNGEVKLSQQHYIGELLQLHQLVNAKPQTTPLPPGSRVLPAGNSNVELAHSTKYRALVGELYYLATNTRPDIAQAISVLARFMGKPSKADMGLALGVLRYLAGTKELGLCFGGDGDLAMVSYSDSDWAGDPATRRSTTGYVFLLGGVAISRKRQLQRTVAVSTMEAEYQATAAAVQEALWLRKLAGYLGLGSEAIEIQTDSQGAMSTGNNPITSVRSKHIDVQHHLVREQVLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5Z3</accession>
    <name>A0ABQ5S5Z3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glycerophosphodiester phosphodiesterase</fullName>
            <ecNumber evidence="2">3.1.4.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_008426</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a sn-glycero-3-phosphodiester + H2O = an alcohol + sn-glycerol 3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12969"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:83408"/>
            <dbReference type="EC" id="3.1.4.46"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.46" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI65000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620:SF7">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE GDPD5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43620">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="9">
        <location>
            <begin position="77"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI65000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="529" mass="56550" checksum="4D1BA04479215DD0" modified="2025-10-08" version="1" fragment="single">QFFISSMLRRSTSIAQQPIRGAQRSKRNPTSPRCSILFICLVANAAPLLFGVLLLICMQGVQAIGDAECILPVDGSPFVMAYRGASADFPEHSIGAYRAAIEQGAQFIECDVVLSRDLVPLCRHEPNIAQTTNVLATRQRGTRNAETCGGVGGSGDGDLSPAGSVVWASDLTLAEIKQLLAVFPEGEVNAAAIPASEAGKHQNQHQHEDWVSGEIGESMLGGNLDKCEAPWVLKSREATPVSENEAAWGAKSLDDEVHTPADLRVASLEDLLQMATVTEPAVGVAVHIQAASWHNSALAVQLKSQNDTFEDLLLRLLQRYGYAKDPYGSEAWRRRPSYIMAFEVGSLRYLSQRTCSPLTQVIGPTIPDTGESWESATSPEGLARIRSYAGSVAVARQELLQAWPPASLQGRQAASGGVRQGIMASDLLFRMRRAGLQVFASVFKPSPREHLLSQAETWVDEISALVLGRGLHQGHDVRGGEADSVEPSASPRQQGAAIDGLFTDSPQELHGVLKGLKCSGTGGGADGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S625</accession>
    <name>A0ABQ5S625_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="157" mass="16518" checksum="4558CF9F2E0FF93F" modified="2025-10-08" version="1" fragment="single">PRPPPSPKAPPPSPPQPIPPPRPRPLPISVSPSPPSPSPSPPFPSPRLPPRPSPPIPSSSPAPPSPLPPSPLPSPPPTKPSPAPPPDDIGGPPPYFEDYPPPSPPPPRPRSPRPPSPPPIPRPPSPVRLRPPPSPIEYPPPWIEPPPELDSRQPPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S630</accession>
    <name>A0ABQ5S630_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008364</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37492">
        <property type="entry name" value="SI:CH211-171H4.7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37492:SF4">
        <property type="entry name" value="TSC22 DOMAIN FAMILY PROTEIN 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1459"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1493"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1791" mass="181835" checksum="DD9E78D738B688A6" modified="2025-10-08" version="1" fragment="single">PSLSLPVPPCPSCSLLVPPCPSLSLPVPPCPSLSLLVPPGPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLLVPPCPSLSLLVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPGPSLSLPVPPCPSWSLPVPPCPSLSLPIPPCPSLSLPVPPCPSLPLPVPPCPSLSLLVPPALSLSLPVPPCPSLSLPVPPCPSLSLLVPPYPSLSLPVPPCPSWSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLPVPPCPSLSLPVSPCPSLSLLVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLPVPPCPSLSLLVPPCPSLSLLVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLPVPPCPSWSLPVPPCPSLSLPIPPCPSLSLPVPPCPSLPLPVPPCPSLSLLVPPALSLSLPVPPCPSLSLPVPPCPSLSLLVPPYPSLSLPVPPCPSWSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLPVPPCPSLSLPVSPCPSLSLLVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPYPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSCSLLVPPCPSLSLPVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLLLSPCPSLSLLVPPCPSLSLPVPPYPSLSLPVPPCPSLSLLVPPCPSLSLLVPPGPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSLSFLVPPCPSLSLPVPPCPSLSLPVPPCPSLSLPVPPCPSLSLLLPPCPSLSLLVPPCPSLSLPVPPCPSLSLPVPPCPSLSLLVPPCPSLSLPVPPCPSWSLLVPPCPSLSLPVPPCPFLSLPVPPCPSLSLPVPSCPSLSLPVPPGPSLSLPVPLCPSLSLPVPPCPSLSLPVPPCPSLSLPVPSCPSLSLPVPPGPSLSLPVPLCPSLSLPVPPCPSLSLLVPPCPSLSLLVPPCPSLSLPVPPCPSLSLPVPSCPSLSLPVPPCPSLSLPVPPCPSLSLLVPPCPSLSLYVPPCPSLSLLVPPCPSLSLLVPPCPSLSLPVPPCPSLSLPVPSCPSLSLPVPPCPSLSLPPSQRGVLISEILGRQLLESMKAVPRRYPNLENNGALHHVSDSSSRNDGWRLTYLDCSNNPQRYSHILLTSTEDIGAAPVGVAPLPSSLPPDRSPTLFESVILTGQGRDLRHLVAVRADTWSRYGLRLLELKGWCHQLVTLDLSEHFAVPASSGCRSHGSGATSVPRREVLEFGAVLRGAVCRLRALRVLRLQGVDLSGFFNDGVLPKGPKARITFVGGGSDTDQVASRRRDGGGGMRTEAPAPDGDGTNSSHVSGSTSQQPDPDPAWLRHVGHTLRALRHLDLSGSIGFGSRELAMVLSAATRLTHLGVSRCRGLAPYGGTAAVTAAAASGGLLLLNLRSLSLGWGFNAAAVQRLCAVDAVDASAACLTALELGVGACVDDAGLGAVAAVCPHLQELRLTLCAVSGKGVGAVLAGCPKLHVLHVIGCVGPLHRGQLLERLGWLPRDGGTAAMVAEGCAIARWAMSDLRLVASGATDLYDEDVLSLLYGRSLNGCLLRSCRSDGFDSAAAAAAAAGASDRGFSSTRLRFLHTLAMVGLPLLTDAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7P5</accession>
    <name>A0ABQ5S7P5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009270</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057948">
        <property type="entry name" value="TPR_TRIP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25579">
        <property type="entry name" value="TPR_TRIP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="E3 ubiquitin-protein ligase TRIP12-like TPR repeats" evidence="6">
        <location>
            <begin position="219"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="4">
        <location>
            <begin position="264"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="725"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="983"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1094"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="983"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1095"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1162"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1197"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1213"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1239"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1268"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1294"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1320"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1331"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1341"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI65666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1357" mass="136941" checksum="CDD551FCC3FEB41D" modified="2025-10-08" version="1" fragment="single">METRRSSKRKEREEAPPSGTQQTSRKTRRVSKASPAAPNHEASEVAGSGNTQQSASAREIERSNSPSGGPVTRLRSRRAAAATAQAPATTAGRTKRNRAKARDPSTEAEERQQLKEETEAALPQAAPPISQPAPAPAAPALASAPAEAEKASSGARRLTPSQLREMANRQGRGQGMDDEKDLAEVFGRNFSGAGSALQGLLRKLGAGFDDMMPMGGVSGSRMKAIMQGLRQTEDEGAQLSSLSELNELLSISSEDNLAAFPTESMVPLLIQLLNSEQNPDIMLMAARALTFLADVLPQSCGAIVRHGAVPAFCARLLTIEYIDLAEQSLQALEKLSHEHATALLQNGGLLAVLSYLDFFPTGVQRTATATAANICRSLAMSPSAASSSTAQAAVKEAIPILTGLLHYSDAKVVDNACVSLSYIAEASAGQPTLLEALTSGGLVAQALQLIGLSETGAMTSQLSLSTYYGLLKLLATAAASSASVAVTLLGTGALSIARTLLATSPLLMTGPNAGGGGAALLRTPDQMHEVLGLLSELLPPVPDSQTLMASGAAVELPQAQTQSGEGVAVAGALLGGGGRQGPAALAARGGELLAYLSANPSVTAQLCNDILPLVLSSYSATVISEVRARALRVLLQILVACPAAQLREALRNLPLAAFLAALLAVRDAATLAAALHCCEVLMAQLPDVFKHVFLKEGVAHAIEQIASSTPATPVVGVSSPPPALSASPNAVAARGGTTASTVEESRRAVARSPSLAATREEKVQPPPATTTAAAAAASPVAALATAAANAAAAFRIPPSSPGGSSLRVALAARAMRFKRAHYDSGCGAPETEGLVRVRSLLQRLPSEPTVTLSQLLMVMAASPTSTCMSSSVGLASDPPGILPPGTSVSVFELLNSGAVRAVYDFLTGADLSAAASPTNVDERATTILRRIAALTRAGLTPADGAPCNPPLVGLVRKLQAALSSVETFPVHYGRAVPPSGSGGFRSGSGYGSRGGSAGSASAMNPGSLSSGLSLLTHPFKLRLCRHGSDNSLRDYSSNIVLIEPLATMMAIEEFLYPRVYRGTSAAAQQQQGSAAAPGGGGNANTTAAAAAAATGGAGDGAGSKGSGTGAATGGTSAATTGGGNGGGTAAAAAAAPLPGSRSHPIPMDDGGASSRRVTRSQAARERAEAEEAARRRGALPVPAGRGSKRRTGGAAPGDSSSSKDGVSGVSTGTVGGAAGATGGTGTGGGGDRDYRMTDVGDDADEEEDAGRLGRRSSRGRVGGGGSEPEVDDDDMDEGDGDADMDDAGGGGDAMFDEDDEDFDEDAMDGDDVDGDIGNLHVHDLHVDQDRSAAQQPSSGNAMAPQAGSGAAAAAAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7S0</accession>
    <name>A0ABQ5S7S0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008744</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="635" mass="65659" checksum="12804EBAEC331CB7" modified="2025-10-08" version="1">MMRCIAWSYVPDGIFSARSLCVAHNQNVSPYVRQRIGLPGGTLGWPVCSGRSVWPHSATSAISSRLSRRKAPGRPDPRVGRQGIADLCPTGVALSAKHAFVHCTDSRSIPRRVAATVASASGSLSGSNNDGGSSNATGCRDTKTASQSSGVMASSGDGGSGGSGESPSAAVASAAVAANVAAAAVVGAVASAAPPPPLRATALGLLQRVVALLRSQFLAVLLLYAVKDGVAFLLHRVTQRMTNHAAEVLLGVPTSSAGNPWWLYLDPTFIRAHPGYELLIGLFFLACLPFTIALNSLVFTITALICPPKQLLKDAVSLNSASSSSFSSSPYPSSSLPPPSLTGPPFSSSSSSSSSSSSSSLLSPGSMSTRIGVGESMYLPSDDPAAAALADLGIHALPTVPPPSRQAGGSPVTIATLSSGSSPLFADLPPSMSTISAATTLAAVPFSSPLEAEAVEAGGKGATTPEGNGPWDSFRTALASVAAALPDATRAFRRVWWVDLQFNARALPLQGLCLLVLPALWAIPRLMRIQLALPVAVLEARSGRDALNRSSNLMTSSIAAYSWPFAALLAVGRVLEYVQGLALVLIPPRWWREVVEVPLLIAGAFLLLKASVHRLQDLLPLAAYLELTQQEAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S816</accession>
    <name>A0ABQ5S816_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peroxin-5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_009779</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxisomal targeting signal receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024111">
        <property type="entry name" value="PEX5/PEX5L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130:SF0">
        <property type="entry name" value="GH08708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="611"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="713"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="747"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="781"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI66060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="852" mass="89832" checksum="AFF77130692101C7" modified="2025-10-08" version="1">MSSLRDLVAGSDMCTPADGAGPSNALGGLVNTLLGGAGKTQEQLRELPGMGLGGAGPSTFTPPIHTPAELAMAVPSGLQVPGLGPPRMDAAAAESFLHGIHGAPFASAAGVSAAAPAEWDAIFHGKEPRPMVPMTAMPPAAQPLAELMPRGPPPLLAPHFQAFVRSALVPGAAASAAGPLPPELAAQLSVADKVRIRDRSTIMARHLYADQGDKFADMQVGHLLASLRIDPRELPAQLNHVHHADWHEAWRAATGAPSPSVAAELAAAGGAAAQLPGGPAPWDAIWEQRHGPAAGPGGPVALMRPPSTGWATEFVAAQAPQPPAASWVTEFAASTPAQPATETTASTWAADFKQEQDQAAATSTATDQQQGQQVTRGSTDARSTSAALAAVLSQASDPKMRNSKFLQFVSKMSKGELILEDNKVVERRPEAASWAAEFGQQQQQQQHQGTAGLSQWAAEFAGQHPGVAGPGPSSAAIGRGGEWVEEFARGVSDLRLDEGLDAATAAQLEAAWRDGEASSWAHEFNKGDEQQFEEWERMYGHGAVEDSLLGGGMAARMGLGAAPGEYVFSEQNPFLGDPDAQQKGKDLFRRGILSEAALALEAEVRAHPENAEAWRLLGTAHAENDDDRQAIAAMMRAHTADPRDPEVLLALGVSHTNELSQWEAVRHLRSWLAAQPPYSALDTAAGEAPDTSQRLTHVIRLFESAAALAPSDPELHVALGVLHHLGRQYGAAVAAFHRALELRPLDYSLWNKLGATLANNGRSAEALAAYQKALDLKPNYMRAWTNMGISYSNLGDYDRSAAFYVRALGLNAAADHVWGYLRTALACSGKLELMEAVENKNLAALQEALPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8I3</accession>
    <name>A0ABQ5S8I3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="25240" checksum="2490D46BC4DA0070" modified="2025-10-08" version="1">MDIDHDLPPADIPLDMETLPEELSAAIFAAATTVSTPQTLRSGTSAQSLRSIANQLHARTSKFLKFIEGIQALVSNHLTPGAVDIPGMAPAAAATTCAAVAGTSSRPPGPPHTGHEDEDMACDDPDEETPAPPTKKPRAASVRPGITGHEDQPRQVKGAGKSKRGGGQQGRHLAPKGGHVQKKGPKQNLAEYLTTFKCTRREFHFRKHGMCLRCGSDQHRVDSCPMLGAEKNKGKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8L1</accession>
    <name>A0ABQ5S8L1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA ligase</fullName>
            <ecNumber evidence="12">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_009081</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="12"/>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 12" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="15">
        <location>
            <begin position="631"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="178"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI65526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="900" mass="96267" checksum="9642C6359D60C017" modified="2025-10-08" version="1">MKQKSIGAFFSGISQSGKDSATEKEVKPVVVPANDKSADGNATSNDQENESEQLVGVAKPARKRLRRNIIEDDDDESPPPAKVKTDGGEASGASPRSPSLPPPGVPSKDELPEPTSAVKKFVQPAQQEVDNVDKVIAVAAASAPSLNSPTSEPAATTVAATKPTASGAPLAPIFQRPAACKAAARPSSMEGAAADVEEEDAGDEDPISDSDEDAGGEVNEILAGTPKKKKAKRAKTAAKGAKGAAAEKVEGVGTGAVAAASKYAEADISKLITWKTGSPVPYSLLANTFEAIAETTKRLEIVAHLVAAFRAILDTNPADLLPAVYLCVNRVAPAHTGIELGIGEATLIKALCEATGKNEASVKKAYEASGDLGVVAVGARSTQRTMFTPPPLTVANVLKGFRDIAQVAGSKSVDIKRGMIVKMLVAAKGNEPGYIIRSLQAKLRIGLAEQSVLVALAHAVHLHREGVKGKDGRLAEKLEAAAQVVKQAYSECPSYDVLIPALLEEGTTDLLSRCHFMPGVPVKPMLAKPTTGVGEVLEKFTDTEFTVEYKYDGERAQVHVVDGGKTVHIFSRNAENNTPKYPDIVARLRGLLKPHVQSIVFDSEAVAYDPANKKILPFQVLSTRARKDVSVGEIKVQVVLFAFDCLYLNGESLLHKPLTERREALYGALTEKEGQLEFASAKTSRDVEELESFLTESVEAGTEGLIVKTLADTYEPSKRSSHWLKLKKDYMEGVGDTFDVVPLGAFYGKGKRTGVFGAYLLGIYDPDTEAYQTISKLGTGFSEEQLQQLADAMRPHIVPQPRPYYKWSDALVPDVWFDAVAVWEVKAADLSISPVHKAAVGLVDPTKGISIRFPRLIRVREDKLPEDATSAAQVAEMFHSQAVIQQQQAAKQMEQDAEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8M8</accession>
    <name>A0ABQ5S8M8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009961</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="40"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="568"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="443"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="622"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="779"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="871"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="943"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1145"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1392"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1556"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1674"/>
            <end position="1701"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1110"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="627"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="817"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="871"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="949"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1147"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1162"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1392"/>
            <end position="1403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1735" mass="177548" checksum="6469D7B067304B5F" modified="2025-10-08" version="1">MILFANDTPISSDVCIPCRPALRTTEELKQIKAQLTNCSFFADWADDSLRQISGVLGCSNFAAGEKVTEPGEAVYSIFILLSGSCDLAVPHPTDPRVAPQHHALLPGDVCGCEAVLSHDPRARGRVTAGPSGALLATLSGSAWADVCARAATGSPAWVATHSDLLCAFLRQLVSAGSSSTQLQQHGGARGGPGGGSGRAEERSSGQVAAVTEGEAEGPAEALRLELIAVLLACFQSVMAAMPQSMLREAAKGCYVRTVRPYGVLYRGQNAPKFQAVLLSGVLQIRTRRPEGSSSSNFSISNLEALSGRAIDGADMAAVESAVTGAPQLVQAALGANTFQRPASPSSALAAAAAAGRGTGIKAAKASAFETAESFNTADNGAGGHVGTANKDFGVGRSRGAGGDGGKADPAVTAATITTTIDGGGKDTETAPEGGEDDLTAATLEEQEEEEEEEEDFHDEAEDAGFAAQGGDGGGEMRGPVSAEDHLTQRFGRVVAAATRGSVLLELPLASITRRRGHRHKAHKDTALTAGLSPATVLIVPLRGLRRGYAAACTALVSSRVAFLSSCKALSGLSRGDLAHLALAARPLWLAGGAVVARQGDPVNALYLVQEGEVRLLDDPDGIVHHDHDHHHHHHHHHHHHHHHHHQHLQENHHHLQTQTHRGLLCKAEAATRVSASSVSLSASLWEDPVMSSEITRPPWSVASHSHMYGHGYPYGHGAAAAAMVAGSGGSSEASAAQVQRSLAQLVPLMILEPGALLGESVLGCGPALGSEEPPSLLPVLPLLPLLPQDPPQEEGKDSGSTAVAGSAPRSGQLHLPHFPPPPPLLPAPPTALPGATTGRSPLQLRQSTHRISFSTGATAATAAIAATAATSDASSSLESVASAPTPPSCKEQMPLQPSAPQVHLDEASPPPPPSQQQQQLYDNSYNHHKQMQHLHRLLTKQGQDHRLQPTDNHNNHGQQPADSSNAEAVGNHATYGNTQGHGAPGRRISGVTPGGISQPGLQQHVQRRRSTETQLGSPPPFVAATSSSQSGGAVAAAAAGATSPSSEAVVAAAVSAEDAAAVAGDAAVAVRAEALANVFPATAVAVGTVRLLALPRKALMWLPQLQPVLAALASGQLRILENRRRDAQEMRDRLEAATGAAREALCTASPTGTASSTSPVKDPYKEARRRQSERQAAVVALNGNAAAALRVPEVLPTDEMISRPSAALEKMGFKVPKLRGVLAHSASVSVGAISGHRSGDSRRCSDHQPALQQLDTPFHYSQPNSPISAAAAAARMRSSTGELGSLDVTSRAQSVTAGVLPKLPATARVAPSMAVSGGAAADVDSEEVLSPLPHGAAANSAAAAAAANGGGYVLGVDSPDRLRSSISMPERSSGYSLTSRLSTVLPRITKGQVQSLRGSTSGSAHVERSLGGLAPPPNPRSCSGSPIRRSPRVYDMDAYIASASVSRAGSVSRSSRGRVGFEVPPNGDGAVLSCKLPSAVAGLELVASASSPRSLSLSGVAASGGATGGSTAATLAATAAVPPPQVAAAALPPRTVSVTNLTRLCAPMAGLDEGTISRTRRTGSGAVSPVSTAPATLPVPPPPPSAATGWRPIRISYDDGRLPVLPSPQRPSRMFQEEAAAAAAALLRGHAGAGSAAVTAVPLVPTPPARPSGKAVLTDDAAVSRWMEEHASSFVNGAGGASPLRRTIPGQSMSQQPLPPPSVLQKLSAVAAGTGSMESHLAAAAAAIKRSSGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S940</accession>
    <name>A0ABQ5S940_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Progesterone-induced-blocking factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009654</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026205">
        <property type="entry name" value="PIBF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18950:SF0">
        <property type="entry name" value="PROGESTERONE IMMUNOMODULATORY BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18950">
        <property type="entry name" value="PROGESTERONE-INDUCED BLOCKING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="744"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="368"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="448"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="615"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="766"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="778" mass="83999" checksum="00B82AA89594D0A1" modified="2025-10-08" version="1">MSTKVKSKIQRLLKQLEQDEDTDNTSALGTGREEEAGDLAVLAPTRRAAAAIDRSPGNLSISSVDAASDNTATLTGISTSEAPLEESESGTPSLSSLDSTPLPLRPGRSPLGKRISYAAAGQAERVAVLERKVMALMAENRTLQTAFSEAMARKNAEVSELQAHLSGAVGELSSRQQELEAAAPLLRLRLDDFRAQLRDLRVSDSVYQELRLLPRESRTALDEVRIAVHEALSESKSEAELLRTALTAARETAARAEDAAARARAEAARNAAALAERETDLTLVTEGMQARIDRLASELEAAMVSAQLSSAKGAMYDELRAKLDATVAENQRLAVVEATSKKLEMQAAQDRATATQKEHTYEILMMDKAHLTKQVDFLADQQRKLEAELELREKKITEVTRQKNELFEKLMAAETLKGRDAEVRLQKELAALQERTHADLEKIRVETAEAYEREARLLRELRDSAQEEALRAKGALAELQGLHEKATLAHAEITRRLEQQVVALQTELKQKSFELSHFKVVVAEKEQLVGRTNMQNELLQDKLSVLMDKYRALEVEHNTQLNKTLAAAAEAVAASSGSGGGAASNVAAAADNAVAAIIAAAANPGALPVKMQVDMALLKQERDALTREVEHLSGRLAAAHASLRLASQPHAFLLEELEAVKLRATQAETKVAALQVALAARSNEVQAVTAERDALRSDLDGLLAQRQALEGMKAVVVKAIAGGLPQVQQAAAMSAAARKSAPPFTSTTIIGEGGLPLAPPLPTAAPDPSSRQQQQQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S990</accession>
    <name>A0ABQ5S990_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad21/Rec8-like protein C-terminal eukaryotic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="2">
        <location>
            <begin position="354"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="404" mass="43074" checksum="74ABF680A3351024" modified="2025-10-08" version="1" fragment="single">GNDAQQRKEQHQQQGTTAAEDEGEGEEQRQQPKRSNCKGASERLIDMERRVKEQDLEASEGHRIDGRLWGAFHPADAAVGEAEAIMQAMETPADEQMQTEDDIDDIEMERLRRHDTPDGSDMERQHRAAAVGLTSLGLLAGSSQGPPTAGKSSKATSGSDESSLGRETEDEVMAKETYRRPSGAPKKRMFGTGALEDLLPAIDEVDVHFDHELLAFMDLDEQQEQGPDATAVAAATAAAGGNSKGGGRSEGASGGVSRKSSHQAGSSARGGSGSEKEAADATPFFCLKETNGLATQVLPPQLFMSRNTKVVISIFRKQLAQAQSQAPVQVAEQHQGAGHSNGGDSGISDLQEAALSFLGLASRLKRSEAVKLFYQTLVTHTAGYVVASQYEPYGDITIRAAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9M0</accession>
    <name>A0ABQ5S9M0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyl-accepting transducer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010007</name>
        <name evidence="3" type="ORF">VaNZ11_010012</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="2 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000029">
        <property type="protein sequence ID" value="GLI66260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000029">
        <property type="protein sequence ID" value="GLI66265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="43886" checksum="47175D8559683C1E" modified="2025-10-08" version="1" fragment="single">LVDCAQESADEHGEVQQREAAAAELRATVVELEKRHEAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRRETAEAGAAELRAAVMEGEKRREAAEATIVQLRAMVVKMEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9Q8</accession>
    <name>A0ABQ5S9Q8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01228">
        <property type="entry name" value="EGGSHELL"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="245" mass="24020" checksum="A4C8124F629C69AF" modified="2025-10-08" version="1" fragment="single">STTGSGGAGLRAKGVGARTHGSLKEAPASFLRDQHKKVTPRNTSPCGSDMSGMDRSRRHHHHHLIHNHDHEPTSPSMRDAGGGGGGKATGLGGGGGGRGSSDTNGSDSRLRAARHDPENREAQGSNPTNTHNGNNGNGNGSSGNGSNGNGNGSGSQGNGSQGNGNGSSGNGNGSSGNGHSGGNGHSGGSGGDGGGGSGGNGNGHSIKDSNNPVFNGNGYSSANGTSQHHSHHHHHHSHPTPRSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA44</accession>
    <name>A0ABQ5SA44_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_010695</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000039">
        <property type="protein sequence ID" value="GLI66745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08529">
        <property type="entry name" value="STKc_FA2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF7">
        <property type="entry name" value="NIMA-RELATED KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="9"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="285"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="388"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="404"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI66745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="523" mass="58357" checksum="E57756C791AFF79C" modified="2025-10-08" version="1">MARSTYDDFVIKEKIGSGSYGVVFKVIRKVDKHVYAMKEIDLQGMSRKEQEECIRETRVLSSLDSDYIIRYYDSFLEKGKLYIITEYAANGNLHDYIKKQKSRLTEELIWKLYIQILLGLNHMHSKKILHRDIKTLNVFLDEDVNVKLGDMGVAKILSTNTNFAKTIVGTPYYLSPELCEDKPYNEKSDVWALGVVLYECCTQRHPFDADNQGALILKILRGKFPPVTGYSPDISDLIKRCLTQNANRRPNTFKLLTLPSIRQKAEELGISLPDQASLALMADRNMAGGPGKARPKTPQGDGAVQEAAAPSESGDALASTIRASEPVGTRPVDVTVTSVERTSGEGRSVGAAPAPVQAAWTVEETKPKAQRPPAWGLVQQMENLDVEDEVDDEDDNPYLNPAQGDDEDGEEEEDVEGEEEEEDYENGDFVEGEGGEDGAGWAPDPTRIAHMKSALMIQRAACLDLVGEKAFNELYALLKSNQVDEASMTDLSRLVFKIIPYDKSEVIQMMYKLLYLESQLEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAY1</accession>
    <name>A0ABQ5SAY1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_011241</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677">
        <property type="entry name" value="AP2 DOMAIN CLASS TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677:SF252">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="1017"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="122"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="288"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="513"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="553"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="621"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="666"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="872"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1069"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1162"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1254"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1305"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="201"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="309"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="351"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="623"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="689"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="882"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1079"/>
            <end position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1390" mass="139955" checksum="14318172B180C9B3" modified="2025-10-08" version="1">MQAPPFGQTPTSAVYPWQIYDVFYKSSSSGATASGAAAAGPGAGGIPPLGRRPSGQVVLSPHVQRHGGSSSRGLPQGRAAATSQQQQQQSSPHRLSNSSDGHAGVTVVPTAAAVRRYQLSELPTGGGASSSGGSGANPGVTPRIPAAAPSGIRRQGTTSIDPQLPQRAVRALQMYPPQLPPRPPGQTHSARQLAAAAAVAAAAAAAPSPQQSTPQLSPREDRGRPLAASLRNGVLTLQLSLADVKELSAASCNDSAGYAITPMDLSTGKALGSSYLISTASLLATAKAATGPGSEHDAAAGAGGGGGPQLRQQHSLQPHQQHHQTAAAGLTATLRPQRRSGDQSPTGGGDAAAAAAADAGAKSPLPAENDKALVVVDGGGGSGSGGGGGRGGKGVNGGTLIDGEEEDGDGDSDGSAGVRGGGGGGRHGRGAGGGGGDARPDIMLAGSGGANAPNTAAAAAAMVAHAYRTGTPEAAAMAIALLRQYHIQQQLLRNRSHLAERLDLDLDPEGLYEQDRSRSRRLQSSHLAAGRRQPRGYFQNDGYDRIVRRENSDEPDMTVYGGNPPLPSVPRRVDADGERYDTLRSVGQYPGGPHEEYDGVEHPGGGGGSWNIVRRRASGAELEMAHSKRGAEAPLERRAGSLSGDPALRQNPRAEQHLHSAAPYHPQMGQRLHSGAVHGGAPYQGSDDPYDHSDEHLERDKADVQVVGEGNPQKRRRTAAAATTGIPPSGMAVAAASRVPPSRGTTQAMSDLNALRVAPWSGRVLDASERAALVKYGLPLAVSQLDAGVAMPAEAATGSAWRSDGADPSPYIKAAAAAAAAITNRTRYGATGGAAKGVMDQIAAQGIPRDGAAAAAAAGPHGGGLGGALRYGTRRNVGGSASPPNGQTGLTQQQHQMRSLPGEQDIATAPLASPTVMEAKDSDMTESIGASLSNHGGAGAVAGHGGTDATGEVTEHVGAGGGGGGGGVTAGGGNAPSQLVVAPSPLTAARDVVELSPKGETGKSKGGGAARAGGAVRYRGVRQRPWGKFAAEIRDPFKGGRLWLGTFDTAEEAARAYDAAARSLRGNGAQVNFPMPGERGYEGRGEEAATREAGDGAAAAAAGDPNVSTADRNLDALAELSGLEAIAAAAAAVAAAEEAAEALRAATATVEAATLPAASTAVAAGDGGGPESVTAAASPLRGDGEPPMAADGSMEPQKLGGQAAAAGAGDMEMGARTEAAAEGAEQVREHSLLNWHPQLTFGNDDVAQQRLTQAATRGPEGRDDGGSAKEGFAQLPCPSVRLKIEPMEYEQPYGVVLRTSDGELDQDAPMAEGNDDQQHMLDSAPKCTTGEEVPYDSEADVSPEATVAATTGGGGGVVADGKGNSTEKAGRRDAKEGTGMGEGRPLRRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB15</accession>
    <name>A0ABQ5SB15_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000037">
        <property type="protein sequence ID" value="GLI66634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48447">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48447:SF1">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="703"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="839" mass="93231" checksum="58EA09313FC73E1E" modified="2025-10-08" version="1">MEAGVRQGCPLAPLLYLFVAQALFSWLKSQEIGLRLLPSDEDRVTACQFADDAEVALEGEESVPAFLEAMDTFARASGQRLNLDKMEILPIGAVDDHHQGSTINGLKVVTTATALNLPMDNQLQPPQPDWPEMLALAYTRMQRLAHYPLSAFGRAAAVSNYCLQTVTWHMEHGGPPPDTTLTYIERLAARLVDRKQGPEDQGQRFTGVPSRLLVGHPSTGGFGLLPLRQHIRARQAMWAVRFIRGALMARRERERVMLLDTAMNTVAEEEEEPVTSGNVRSLHPWIRTLATYLEITHPAFKPWALTTAQATGPWLGGETLPEEISRIVGALGHLPPLEDVSNPPLAPGDWCWAAALWGNPLLPNNSDNGGSINGNNHRPGLETRHPLMASCRKLLTVGDLVRVYDMLEHDQIPLPQYMTWLDWTQQFLDPSASPTQLRDKESTRMALSALCNDISPAWIAAARKVVNRLQPQQPDRVEDRMQQQMPAPPDRTTVMAMIVRRLGWRLPGDSGIVPLYNLTVSQATQLQGVSTAEERLELLSAFVQEAQGMPTDGPAPPCPVTNLQRFQATLDRLWTAVRWENKNKETLWRLVVDGIPIPGNTHLSRVPIKPCGCGSYGADTDQPCSPRLHHFWECPIAKAVREQIDRHLPLPTSATIEGGLTRQQLWLVQAPPGCEQAPWDVIALAALSSMEYGRHCLRAATREHRPQWPSTHRRRQQQQTHTAEQGPRQRAITDFFNRSGGFPQEEEEDANTHPIISTTQPAMDTPTQTATSPLDRAQARTVADFWARLHSFAALGIPHYGWDQVGPQHPILAIVGTQMRCADPVDITNHGAARTEQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB73</accession>
    <name>A0ABQ5SB73_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="157" mass="16465" checksum="3A237DD99A54905B" modified="2025-10-08" version="1" fragment="single">PDPDDTGLPNQVSGTRCTGHAVRIDDAQQGVYICVCVCVCMSISIDQSIHLSVGPRYATLKQVGKSMHVLSDGNVLKKKQVQDGAPPPPPPSSSSFSLSATSAAFDAAAPNGPHQPAASERIAAAEGLYAESLEGGSLHQSSRPSASPRFQEQRKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBE1</accession>
    <name>A0ABQ5SBE1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Armadillo repeat-containing protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22895">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22895:SF0">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="50374" checksum="6DE954303F6CDC09" modified="2025-10-08" version="1">MAQAWPKAKPKLTQEAFDEAVSSNIEDFDMSREEAIKSAVEEFNMQDYDLSGVVINMAGNDLQSHPIAVACKGLRDACSGQGSLPEAIDALSSSLQASTSDSLDAGASLSQEASVIAHKGGAMQALLEASKQSKSHTDQLLKVLKLLQVLLGWLETRDAFTDAQGVELLHDLLRDEDSSPEVKTAAATLAEAAARQVEEAKCRLVDSGTVATLLEQLQAPDAPLDFVGAACGVVRSVTTADDERPPASKAFMHARILAKSHAAIRVLLAVLRRVPAAQEPATACRVLGTVRQVAANEEICREFTDDGGVMACLQVLRECAAQRDVCRACLGALRQLAHSDTVKQLLAEAGAVDQAVRCVDLHPGSDEVAEVVLGLLGNMMLRQPEICSKAAEAGVVDMVVEAMQRHPGRAPVQRQACLVTRIMVARNPELRPPFLERGVEALLRTAKASHPALCRDVGSAALRDLGLDNYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBE2</accession>
    <name>A0ABQ5SBE2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="93"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="326" mass="36711" checksum="84AE10333B97EA62" modified="2025-10-08" version="1">MIRLYPVLDSNMIITMMQSVWMGILLNWLGFLVHGKRWCQLLEAEERAGPHISQTGTSFVLLKYLRLDMYSHTHACVRMDMGPPEGPSEGPSIPDAPWESVSMDFVVALPKTEGGYDSVFVMVDRLTKMVHLAPCTSSCTAEQTARLFFDNVVRLHGVPKNVISDRGGQFTSKFWEALCQLVGMHVHLSTAYHPQSDGQTERTNRTLGDMLRNFAGCTPSVWDTFLTSAEFALNNAVNRSTGQSPFFLNYGFHPALPVWRELEINIPAAKTFAKSFLSCMTDAKACLDVAQQRTTDYYNKSKRDVTFSASKKCMMWSEYWIIGFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBV4</accession>
    <name>A0ABQ5SBV4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053202">
        <property type="entry name" value="EGF_Rcpt_Signaling_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34009:SF3">
        <property type="entry name" value="METHYLTRANSFERASE FKBM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34009">
        <property type="entry name" value="PROTEIN STAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="23724" checksum="D96CAEB19327481F" modified="2025-10-08" version="1">MSGTVPTFRSATASNSAFIILESLLMSLILLVPRCTAIRVHFPHWRVLRAATAKTVNVSIPIKELKPFARSQSGEDIRVFQNYLCGLQNGTFLELGALDGERFSNTYMFEYKLGWRGVLIEGNPRSFESLWKKRANAVTIHSAVCSEYQIVHYTMKDQPAVDGILEFMTDSFKESWHKNWQSYTEDEAPPVPCYPFSSLISLAGVFGCGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC37</accession>
    <name>A0ABQ5SC37_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VTT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011634</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053240">
        <property type="entry name" value="VTT_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46826">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46826:SF1">
        <property type="entry name" value="TVP38_TMEM64 FAMILY MEMBRANE PROTEIN YDJX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="3">
        <location>
            <begin position="154"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="391" mass="40588" checksum="AA2683B37853D3AE" modified="2025-10-08" version="1">MAQPLFHQGACPMVKSRYGSTKRLFLHSTLPTGLCGQASPRPASLATQLEEQACSTSSRDVPWQLSEKSLATMNTLRSPSCSSVATWYSRAAIFAVATGLSLVLVGSASANGGTFTGGAFDLFKDLLDQVEAMGPAGYLVFVAMVMLSEMIPLFPTQPLSLASGLLFGGKQGAALMLLGVTLAAVNAFVISRGIGRPLAEKVISMEMSEETGDSPSAATHNAVARKLAEVQRTIETGTFWQQLIAVALLRLTPVVPFSASNYVLGLTPLQLPAFLGGTVAGMAVWSVLYASLGGAGRSLLESGVDMGTVFAELAERSGTYTRPVLLAAGGVAAAGIVVYLVSKQLGARGGSGNDGGGDGGSASSPLTQGRRRVSGGDDRELLIREEQLLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD20</accession>
    <name>A0ABQ5SD20_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012109</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="entry name" value="GTP1/OBG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF31">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="5">
        <location>
            <begin position="198"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="445"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="105"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="325"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="548"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="344"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="559"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI67827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="696" mass="72191" checksum="C6523A0F6D67F006" modified="2025-10-08" version="1">MVTTFTPRFIAHLTKSPGTALLLQGCQSLLPLTSLLRFTGVFFDYSEPCFVFQPRVSHQQPRPQSPTPSRLAPVTAICQTVLLSGYQRQPLLDVPPRLLLKIPNTAPFSTSSSHRNDSSSSSTPTTRNASRASSCSGVAKLQQPSRNDSGDAAVAEAGKAGGRALDGNDEGDLPTDASCRHSHRAAARKGKPVMPQRGQFVDWAQLRVRGGRGGAGRVAYEPVGRGRRLGAAGGSGGGGGDVIIRANNSKTSLHGILPVLVGSAGGAGGAGGRTGARGADRVLDVPVGTLVRIWQASAAGKSSEAGDGLEDLEDRGDAVADYGQPADQEAEVFSPSKGEEESEADAHNDSDEGEHVAGDDRSRFAIGECPSLPPHGADSLRFTSPPTVEVELTQPGQVLVVARGGSGGRGNQAFPSLAGRPAPSDCELGTAGQEFWVRLETRLLADVGFVGLPNAGKSTLLSAITAARAKVGSYAFTTIRPQLGTILYGDGGRLVTADIPGLVQGAHANRGRGNAFLRHIERCRCMAFVLDLSGGESTACQVARFQQTHTGDGAGGDEADGRGRAQDIRPRQTRSHVAAGDGGDGGGAAAAYGNGDDGGETAAKALFGVVPLPPAEQLRVLQEELRMYNPELLAAPALVVANKLDRCSDPRVALRDLMAATPLPIVPVSAAHDVGLTRLKDALRAIARPGTEGFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDH3</accession>
    <name>A0ABQ5SDH3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58022">
        <property type="entry name" value="XRCC4, C-terminal oligomerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="509" mass="55118" checksum="2AD48D155F5676FC" modified="2025-10-08" version="1">MDSELKNLYARAFDTPAGKCFSMIVLGPDGDRNGFDVTVFHARSMRTYIGKGLQAPTRWIKPELWMPLALRALASELPPEGVHFNFDPDTGALSWTWPAREVGMSAGAQQAVMLQRLKDGDAGPLEDMLSTVWESCYRLLVSCRQLDAQLQRQTDSLSRTQRELQDCTDKHKSLVQELHIKFARVLDAKKDKLIEQRTHIQQLEEQLKAARQELAEAEAVDTDEEGEEDAEADQDGKEQRRRLLGQQPENEAPMTRMPAPQSPVAAETAEQLDARNKANPHLIMAREVKVEPSAPAPQRVGARAIYGDCAAAVCKSAPGQAPASFFEDMDWELGVEMMATAGQPSHMIAVSGAKAAVAPDWTTGPPPPAPLGGTYASLGSTQIDDELLAAPTQPAVHIAPPQADSFQQQVPQDLLQTLYERVPPAADHQQLLARQQGTAGSGGDAGLRISDVVAGYARSSSDAAGCPGRTAGGVGVLFGNPYGDDEEEEEEEDDDDAALGLVARRPTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDW6</accession>
    <name>A0ABQ5SDW6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF3533 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_011991</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022703">
        <property type="entry name" value="DUF3533"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051328">
        <property type="entry name" value="T7SS_ABC-Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43077:SF10">
        <property type="entry name" value="TRANSPORT PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43077">
        <property type="entry name" value="TRANSPORT PERMEASE YVFS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12051">
        <property type="entry name" value="DUF3533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="460"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3533" evidence="7">
        <location>
            <begin position="108"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="565" mass="61102" checksum="4A6A2DAEF32117AB" modified="2025-10-08" version="1">MENLQLEKGRKPSPSPAAKPPAPPPTSASLPAPSTKVAIANASTDVEAQRRGDSQPAANLSNKAGAGAPSPVDNDKIKAEADLTSLEAPYFQPYWSTTFFLTCFGAILVIWAFCWLYVGAFWNPTTRLGNLHVVVLNCDLVPGAGALNSSFISGGILSQIAPGPLANGLLKASIWNHSSPVSHLLRWENGSCPGAGGSGRVCGTPQQEADCVASYSDDVLQGGPWALLYFPSNFTASYLSFFPGSGALGGAQNQRPVAQYVYARGRDYSTYTYIGSIILSNLMPGLSQKLTWDVASSPGLLKIISPTFLASGISAKEVNLAPVNNFGQHFASYIFCVLLWLGSSFVVVNSYQFKLKSEEKLLLMQNNNEGQRASAPDGVRVRHIATALMIKGLVAALFMFVLMVFLCIVLWALGKGTEQWYLNPGYAIAFGWYMSWSFISINAVLLHVIGIERFSSVTAFLLILQLTSASGIMTIEVCNRFFYIGKGLPFFYGVRGFRTIFFGTSRNKMYINWLVFTAYNVVFAPLGLLLVTQRVWSGPKRARKQHRDGNSAGVGGIMQPVAMGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEU5</accession>
    <name>A0ABQ5SEU5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="101" mass="11135" checksum="441DEC1981DFDA25" modified="2025-10-08" version="1" fragment="single">FTVGSSDTARIHTVKQGRQLVLDCFANTGTAMLVSWVLVLPYLPVMVRHWLYTLASATFLTAVFDIMGAAAHWTVGITAAPSFDRPWLVRAGGWEVCCCCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEV8</accession>
    <name>A0ABQ5SEV8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_012405</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase CarF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019547">
        <property type="entry name" value="Lipid_desat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48231:SF1">
        <property type="entry name" value="OS08G0187900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48231">
        <property type="entry name" value="TMEM189_B_DMAIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10520">
        <property type="entry name" value="Lipid_desat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Lipid desaturase" evidence="6">
        <location>
            <begin position="242"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI68075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="428" mass="46688" checksum="3A324F41F72E9157" modified="2025-10-08" version="1">MALQIRSAVRTPTALGPAFLPVRSLRHVLARADPPSGGARVTREFKEGDSEVTIPGAPTNASKQTDGLYVDVNEAPRRPKQGNLSKEMKARLRAEYTGLGGAENTVAWEVADDTYLAAASIRRPIIIAAASVQTLEVDSTQRDTTATGLDFNPRPLGATVMDAEASTSIPAAVVGSADLSGKVGSGSKWVIDENENLKSTPEMRAWTWVSIAMIAATLQQATKQVEGPDDLVVFCAAIVLAYILSDLGTGIYHWGVDNYGDGSTPVFGHQIAAFQGHHQRPWTITQREFANNLHQVFGPASYPAAVLLTLSPLMPLGWNAWSSSFLFLVCMSQQFHAWSHMKKSELPPVVVALQDAGLLIGRRMHGAHHRAPFEGNYCIVSGWWNPLLDSTGFFRGLEKVIHERMGVAPRCWEEPQEDWKELERPETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEW2</accession>
    <name>A0ABQ5SEW2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Symplekin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_012173</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021850">
        <property type="entry name" value="Symplekin/Pta1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032460">
        <property type="entry name" value="Symplekin/Pta1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022075">
        <property type="entry name" value="Symplekin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245:SF20">
        <property type="entry name" value="SYMPLEKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245">
        <property type="entry name" value="SYMPLEKIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11935">
        <property type="entry name" value="SYMPK_PTA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12295">
        <property type="entry name" value="Symplekin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Symplekin/Pta1 N-terminal" evidence="5">
        <location>
            <begin position="106"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Symplekin C-terminal" evidence="6">
        <location>
            <begin position="1462"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="338"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="482"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="774"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="934"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="981"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1742"/>
            <end position="1777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1812"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1880"/>
            <end position="1899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="365"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="491"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="574"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="823"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="943"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="982"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1020"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1748"/>
            <end position="1764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1828"/>
            <end position="1849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1899" mass="198352" checksum="B4A8D35945517542" modified="2025-10-08" version="1">MTAIHIRLPPLIELLRSASGEDAQLSALNALRSALEPSSNDSGPCHDDFVATWLPGVLLQHQANVSSDVRRSLAAFTGQLVINHTGVQGIVHSAACLATLMRDNTPGVVKEAVLAFACLLRASLALISLKPSQPDVRDMWDNVRRLQGDMSLFSQAHPSTCVKVAAAKLAEHIVLMFTGDSAPSVPGVPETLVNKLRLPDAARLAADADPQLRWLLEPLRPAAALEQPPARLIAHISAAWSIIIARPNLMGKLLPVLMALAKEGGFLASNSPAAAHCTGADVSVGNALRGGLAAVLKSRVPSSVPWRERVVAALGALGAADTAANMMKYIERQEYKERRALRDKRPAEAPLLNPEPKKPAPGPPAAAAAAPQPVQPQPAAMPAALPPVAAGQYVVAGLTGQGPASVPPPGLQYTILQAGPNGVVALAPYGQPPPGVVTLAAPPQIVGGIPGQQFGIAMVQGAVPQQQVGGPAGQQVVIQLQNPLQPPPQPQQQVQPQPQQLQPQPQQLQPQQLQPQPQPQPQQLQPQQHQQVQPQPQQLQPQPQQLQPHQQLQQLQSQPQPQQLQPQPQPQPQQPQMQQPLPPAQQQQQAAMDSHQLQLQHQAAMQAAAVLTAAAQIAAGMPPVTAPPSDHADMDQMLQYVDAAVRSKDEALLESLLSGLVHSQPALLADLVLGHMPLLPPALPQHLWPPPAPAATTTTTVAAAAAAAPPQTAPVPPPVVLQPSAAPLPPQPIAPPHVAQSVVATGFGTAAPARAPTPPPAPLVAAVAAAAAPPPPVAVRPSGPSAGAAPKQQPYGRRERGPLPPLPGPACPKEMEPSQLLPASKGAGATSGAMAAQAPLQLPPLRPAVLTRLQATTMRQNALKRLMCHAPCAVPGRHMRQVVLARLCARVPPGDATAEQLLLYLLRHYHEGGSELALVWLNALFVQGCPLPAQPRQRQRHGAGSGGDGDGNGPLDYKADFKDGKEEKIKGLYTEAMDTVEDEKADRKKGQDDDAAIQESKGPATAAIGANGTILSTQSRRGDGSSLEQRHGVAAEAGPSTGAGKAALGQEGPGGDRGDAAMPPDLSSTMYETVLLALLERARQHAVTWHAHQLRRYSRGLRETAVFLQQQRTLLLPLQPEARVAAGLEGLVVTARQLQANVAWAAAQGTWLQSEAAGMDLDDDDGGGEGADNEELGAVVPELADEVVTEVEGVQASVQESMMQLRQWQLEMGSQLPLPLPPAQAPRPIVGLEIKELLKAAPSLPLGGVLGCLRDLLAAGGKWPTLALSCAWDAIETRPPVRGPMLELVLEAAEDPHEEIRSAAVRLLTSKLYPRPNLRPTIVTAAALRLSALMPVTPAPAAHTEAEGAEGAATSTEPSAADCTRRCALYVALVAKQPDLLPGLVASYAGGGPHLRSAIAGHASALALALGPTHPALLGQLRAPVPGSEDLLLVMLHALTEKDLPPGRLVDACKAWYAASGDPRVMVPVAFTLSRRDVISMLPVMLRSLQPPVLKRLYRALASKHGEVESLFSAHELLLVLHRDLDPMRDGVPLKSLMSAVDLALHSPEIFPQPVMLSAIRSMEGLVPLPRMFMRTVIQALKAAPRMRLDVAQLLERLVAKQIWTDRDQWRGFLLCADHMRSDAYTPLLQLPEAVLEVTLLGSAASEGARAQAARMGLPLGCQLPQASGARLATYVLQPAPNQPPIANVLITHQMRNILQRLMQIHKAQEAAAAAAKAAKLEATEEASASGPSEAVKIEGLEAGTIKAEPDVSKSTEPGHDVERTGAAGPAVGGAETSGRGAALTIRAGREAAAAAAAAAAASGSGLGLRTGGGLPATKSSDPVSDPASSRTGSRSGTVGASAGRGTVVDGVGMADPIMEDTGTEEGGAGGATGAEIEAVEDGAGGGTNEFAVDFDEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF01</accession>
    <name>A0ABQ5SF01_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF726 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_012459</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMCO4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007941">
        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920:SF3">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4 TMCO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05277">
        <property type="entry name" value="DUF726"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="209"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="339"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="630"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="682"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="927"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="395"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="651"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="696"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="948"/>
            <end position="961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI68120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1399" mass="143536" checksum="9BB5462A6B2D2D00" modified="2025-10-08" version="1">MDLDDGERYAAAALFTLALHTTQVETGVDDIGNCLSACKSAWGCPDEELFDDLICVPEDEPWPGMEVPSGFWGFDCCAPQGLCRRLYHALGITEARWPGLLKMPEVSSATSVHDARVALVRLVRRYGALLDPAIAPLVSRAVSMDLSDVGGGTLGATESIQSKDMLSTPGEKHQHQHQQEEDVFVLMPADDPEANALVRAHGSVDGEFQQGAAASGGRGTSEVGDAEPSSRRWLKQDSETSVSSSPVVARCTAPVSDAAGAATSNERLLQLARGEPGEVDGRKEGDSARDNERSSGDGPPAAGGAAAAATASHEPTHRSVLALYELIGACLRASAAAGDAADEGNNVAAGSRASTEGQKEAPGLEALAPVTAAHGRAEPQQSASVSEGIAHGAEGSPGGSGGEGDGAKGKSGRFSFFSGRLTGSSKGAGATGSGGGGGSDSGKGASSVGAAAAGGTILRVVRTPLVRWYDARARVALRRLCHWLHIPGPKMHAMEQMWALQLLRLRPDGQANVTAGAGPAGTMGNGPRYMAYLKISAGALGGAALLALTGGLAAPAIAAGLGSAIMVFGGSTAAAAAVTTLGGTAAGTAAITGTFGVIGASHAGSKTAALYGEVREFGFWSISAPFILPPPPSAAESPEADRGDSGDCGDGTDTTRANDPSGPSSAVGGSVSRWSSLFRWRTRGGDRTDAPGPLNASGSLPQPQEQENETAEQIDGQRVVMPVPPTCLPPLPLPSPLPRRTEVPKPGNVLQGRPGRTARVAAVPLPGTGTGSDAADLLTTTAVAPSPALARQSRSSSTVSRDGLFIPADLLVEDGVDGNSGGSGAERRMAADLSHPAALFRHAPVTPYPPPPADNDSDALALTLTAVAVQAGDPAAAADQHSGATLASASPTVSSALAAAARSRVASDGGAFGNSGGIREISAHPLYTRSNRSDGGGAPAVDGRSVGATASLRSRSRTTSLGDVAVPNRDGASSVSMLEVREEKSYNRTEGVFCSRAQCAGEQAGDGAGRDAMPAAREWDAELAASDRSAARARLERAGPDGAFVQALLAELDAEAADLDGNNSGCTEPATALAVAATDATSGSCPSSRWSWFRSSNKQPRTTADGEISGSCGATAAAVEGKKWTKWTGKGEQARPLLRVPVDPKRIGESTLALTIAVNGWVKQPEDFVAPWRQLPSSGRELWALVWESDVLQSLDGALRRFIRDKAIEESSKMVLRLGVSTALVTAMMLPMAVMSAINFTLGSCWRLALRRAQLGGRCLAHMLMQGGHGDRPVTLIGFSIGARLIFHCLLELSRCGARGLVESAVLLGTPVSANEARWKQARAAVSGRLVNAFSSNDWVLGVVFRAHSKNSLVQRASGLAPVAVAGIENINLSSLIAGHTQYLEKLEDVLCALNLAEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF22</accession>
    <name>A0ABQ5SF22_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_012273</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07473">
        <property type="entry name" value="Peptidases_S8_Subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051048">
        <property type="entry name" value="Peptidase_S8/S53_subtilisin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034204">
        <property type="entry name" value="PfSUB1-like_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399:SF4">
        <property type="entry name" value="CELL WALL-ASSOCIATED PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399">
        <property type="entry name" value="SUBTILISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="7">
        <location>
            <begin position="217"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1403" mass="152743" checksum="07AD5F157A86A2BC" modified="2025-10-08" version="1">MSINILFDEKDSIAPAAAPERNVPGFLEKLRLGWTKWTAKKPVTAVLICITIITCIIIVAVVPSVCIVRGCGARSGDEGVPADGNEGSSLRLLIHFDDLKKTVKSKVPPFLYDYFEELVLSYGVNVVSFKNYSALYKVQQILENQKDVLILLRDRTLYQAPPPDYFLRSSIPQQVANTVLGRRRQAASTTEPGDELSQEQWALDKVAARQAWNTSLGDGVIVAVIDTGCDLEHPDLADNLWVNPREIPGNQVDDDKNGYVDDFMGYDFAGPCPETYGPGCGRRPNATDFTGHGSHCSGIIAAVRNNRVGIAGIAPNVKIMCLKVTPNDRKFYLSNILVAIDYAIKEGAHVISCSFGPEENTHNPQTGAQRDALRNETDLYRLALNKLKDSSMLLVAAAGNEAANLDDIEYYNPCTLIRDFPDNVMCVMATDTQDKRLEVRSATRIEGSNYGPRTVSVAAPGNEILSTVINHTWANSSGSSMATPMVAGVAALVLSILGAKDANFYKGPQAKSILVESAVSIPNLGNRRIDASAAVQTALLRLTNVIRLVPLKDFNADNQWTTMEGFNLTYYYNKTIAPENIMAEDTKPVGSWCIIDGYKSNNSFLVVRANMYLPKSGRYSFQIKTDANFSNLEVYLGQAQLRNLSEVYVIQSTAGWYDFELRYKNPVKPIRILMADPGAAVNLGNATGIFFITSDAEPRNLYYAPDIALSSAWQVLTRRASGPLNASAITTSSLKIDHGFNTSTVIDDLNITSVGTILGSSANAWTSPFVGIAHTRFRPPPETAGALRFQVTCELCALYVNGLRVVDVYDPSLRTPLQPVTQNSNCITLNVRKPYVSTIHDLVLYFALNGTGTTILAVGWLPCTWNIVRQSLKPYIMNNLLWQPSSGVAGYVPGMQCDVWLSRPDINAQSWAPLIKFRLPTVLAHTAQGIRGSLPGAMLMGSDTDVYSVFNASGCSAMETAVGGNCSAAAGFVLAVRCDRGYKDIINKGFMLSDAAPAYMRCWTYVNGGFPNGTVQTLKPTEANIYLGSQRVLASPDTVSIVFPSYAPNATTMNGHYQLLVMEFPGLRLNSLAGLRNGSGKGFFIVDTVNTLLPIPLISTSTAGRHLQRRRLSTADPSFYDDLDLGLDLDLDLNKTNQLDHNLPNSQYDQQIHEGRQAVSTGNHNRDNDVNNLNQRQLDRRRRLQLDLPANSFYDPKVLGASSYTGAVTPSTRGLCGFSFAMTPAFTDQPVIGPTLKIQTVFYNFSLNAGPVLGTPCNNASAGLSDAVCSFGVTPPSSTPPHRMVQFEGFLRVPISRSAGSGTTGTMRPWVLRVTDDAAPLQTTSITVGDVTVRNGVESPNPVRAGETVVWLPGSGNTDLFLPAVVTARIRPAGTQIVFNVTLISPERTAQRVDNNFWYCNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF67</accession>
    <name>A0ABQ5SF67_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052506">
        <property type="entry name" value="Bact_Fn-Binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48234">
        <property type="entry name" value="GH09231P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48234:SF1">
        <property type="entry name" value="SEA DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046263030" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="657"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="814"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="814"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="868"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="931"/>
            <end position="957"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1113" mass="120103" checksum="5FA01207EFA8460E" modified="2025-10-08" version="1">MGRTAAIAAALLLPLLLASTAVGQNQISKWCQIANYGSSPVCTPRQTRCTTVDQYYGRNDPRFDICMIPGYWNGSDTYPYAKNVFTDVCGGPGGVLTVPLKNRAAADVASTPSMATAYIYRSYSSDPTKSLIYITVAINGTNADGAGQIFYQEPYPLDVSTSDVNEDLRIDVSSSIYIWTDARNVSLKQYVNPMMFTGLYTCFTYVFNLRRICDPVRSYKNPAKRNLNEICTCRPEYAGNCDPVDISSATSLFIALTLNGVPYSGYLLQNNSCTTPDPGEQAIVLTNANYISQFVQDPAACNARPPVPPIPPPPPAPPSPPSPPPPPPPLPPKPPSPPPPPSPPPDLTYSATISLMTYDRNRFFSSGVDCFVGRNATAAYYRGRVTVQRIQCAIVSVYQSALDNDFDMIQWTYYFSSVDNLRYFFQSVNIEDFWTDLYVAMSPGCGAIGNYTDSYYNRNNAPPVPLPQRNLVNPQPFCIFGGVVSTDGCWYTLDQFQCPKPPAPPPQPPFPSPPPAPPSPPSPPSPPAPPPPPCSISVNAYFPNFHASTTAEIACMTLRLAMNVYTFNATTWYCDLDKYTSNFLGVYTTYTNASDSALFWTNFKNPYYAVATLNRLNPNCNTFLVATGSPSCGSPDVYWDVRNTGYPVLSDICGPAPPRPPPRTASPPPPALPVPPPAPPSGWPPGGAPLPPPSPPPPPFQAPMVLTVSSNRPYYPGICDAAIKSLTAKLAFFNRPFQGPICNLGQTIAYFMVVTVGFTDPRHALSFAIAFNSEFAEFVTTVGLPCGSTSTIQANGAAYTEACPDVPTLCCASPPRPPSPPLPPSPRPSPLPPTRKSPPPSPPPSPLPPSPVAPTEPGLPFAPRVRRPPPSPRPPSPSPPSPEPGFPDAPESPPPPETPLPPETPLTPRMPAPRRSPKPPSPRPPVRRSPKPPSPNPPRPPPPIVRRPPPSPPPSPVVNPTLTQYRTIAITTPSTLSGTPVTTDLLPILCTNLLAGLRSIFTVYGWESNVQYKLPLDTCPTRNLASGAKQYNITVQMTVSAATTLVTNLNAPANFQLFVTATDILCDSMVSLVKVATQEELFTKNKDVAPSALDPKNTFSTCVHSVLLMRKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFG5</accession>
    <name>A0ABQ5SFG5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037">
        <property type="entry name" value="FORMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037:SF24">
        <property type="entry name" value="POLYCOMB PROTEIN PCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="492"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="723"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="881"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="933"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="728"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="881"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="944"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="960"/>
            <end position="976"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1152" mass="119485" checksum="CD4C44042F317373" modified="2025-10-08" version="1">MAVEGLTSIGALTSLQQVHTSAATGTCISLEVATSWPGFPSNYLRSCANQRAMTVDLDRSCSRAVDGGEGNETPWTFPTTSNATSGLSVVTHDRATIAEVESPGCLVAGATAHTPMQRTLSAPVGAPITWLTTDAFAPGTPPTLQLLTHPSPSGSPPLSAWAPRIPRSIGSMDRAVCHNPLQRNQSLSQACVIDDCSQLTTSTLSPSSLLSAAFRRCLNGLGKGLGHSSSSGDENRCIDRVSPLPDCPFPPSAGPAGPNTMMAYEGDEASSPAARAQLLTSVGCATPTACAATATATAVANVAACDGEQSEGLPGHVYPGEIHGGDGGGVVAVITVAVVSQEDVEMTDAGAAAAPDGGDGGEADDGSGTLLATFGAGGLAPGAWVCSAAQTAAQVALDQVVVVESGGGEAGEEAQPVLLPPYDLNRSSIWRAGNPRGMPPLPRAYRTASRWMPVPPLISPELFLIGSTEPGSPPPDVDTADLADTAGLSKTRAYQRRDEQSAPAPPPLSSCFPRGMASPFGAALNLTPDPDLDSEPLLDWGGARWTRLTSPFAQLAEQQQVVRQHGRDDDKGGWEDPVAEYGHDESYSYDDGYGTCDAGHGDAFVRGASAGSAISPGAMDAVMAVDEVMYGDAAADSRTRTVSMAASAAMGPVMDDDELDDYYLNTLCGGCDPLVAFMQQELARSREIEQVCLQEPKEVGEETADKRLAGCSGGVADDTTDAVEQAMQPPPPSPPPPPPYELQQQYQPAPLEADERIAASAGIVEGTVPQADLQLDGSPASWPVPSVPLPLQCMNTAAVAVPPSQPVAALPEVSSSPLVAAAAAAAASGSHHPIPLSPQSAGFHPSEVVSPLTEVDIEDEELFFELCLADGPDEEAALTEAVTSPRISSARSDAASGTGTVEGGSNTDGCGLVRVTDDAVPLVAFMAVTEADMREPRHSHSRVPHPFSQPLLPPHSQLLPSPPQSPPPPPQSPQSPQPQLLVVTGACCHFHATELHTSHELQLRQHVEALQLQAQQQQQQQLVEEAGSDAGAGLASGGTGCGRDLEDVTDVLCPPPGAAASRQDLQDCALPEVRDAWGEEECGNENDSRVLVVMLERVESCAHCSVAADVRQMVELAVWSNCFWEVGAVAAEGRNTERATTLQEEEQEEALC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFN2</accession>
    <name>A0ABQ5SFN2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12393:SF6">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12393">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="67619" checksum="590D812149300DC4" modified="2025-10-08" version="1">MSKMSLITQDGPHPAEQSRWSQLAPELELRVASFLCPNDISCTIRLLNRAAAALFSSPSHKIVRLSQPVPHHAFSWRWATSGATYSLTLQQRQQLVILTATTGSVPNLAIAVAAAGLVSAANVITAAAAAGQLDACEWLKARGYPLFSCLEAAARSGNVSMCEWALACGCPWRDAVFKIAAKGGDPWFIEWLLTRRPAGLAFPYRSSFLPALARGCRQQALQRISRIYLNCESGKDGERRRGCDDGYADDGDRDDGGRDDGGDSPAAILARMQGMLKAAAGSQCEDWKETFLWLEALGCPSDGNNGSSVTAAAAGVLPDAEDRLRWLHERGYNCNLNTAVEAVRKGNVSALSYLLMEGGLKLNDEDSDVEHLATTAASRGHLEMLQLLHCHECPMDMEALRGAARGGFLDVVRWLVDEPGVYTPLDAVLMENAAYSPSLELLMWLRSRGCPWDAAVLNAAASTGRMDKVKWLIEEGCPRHDDIIASAARGGCMEMLEWLVQGQGYPFVSGWPYHAAGCQGDFAILECLRRLGCPWSGRGMTRSSLFYDCLGSHSTPCAPIEVLQWLHDAGCPVCWMDAWNTICYQQGPDKEEVKTWVWGWLYETLREAARGKWRGARGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG87</accession>
    <name>A0ABQ5SG87_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="343" mass="38538" checksum="DC7253904B98BC5C" modified="2025-10-08" version="1">MHPPTYTAPPQIDQFYDLFWPPCFVIIFSCIGYFTNLVQRLMQHTLRPCSLGSHRPFHGFPSAKCQMMSHQCRAGPESAFSEDVLARLRAAEEEAARLRKELAAAQAIKGTQDPIEKEGKPVRIDSVDNRETLFGGGPRTTWLSEKDVEFFSGPAGVREEDASTMEPEYKATVQRRLVIGAMLALGFGAFALVPTEALRPKPSKPMYFYLVPLLRIQELLGECRTIIEVADWDQLRQALSRIEGPPNNVRQNLDNIIALIPEAKTASRAQELSADLYEYLRSLDYQRYFDAIPQKVISGAQNAQYAQFSLSSLQAAQSKMVELLLLVPKDQLQLARDQLAVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGA1</accession>
    <name>A0ABQ5SGA1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Centrosomal protein of 290kDa coiled-coil region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_013486</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026201">
        <property type="entry name" value="Cep290"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18879">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 290 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18879:SF20">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 290 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1138"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2013"/>
            <end position="2093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2204"/>
            <end position="2227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2503"/>
            <end position="2536"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="85"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="304"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="375"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="596"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="872"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1223"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1350"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1419"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1622"/>
            <end position="1667"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1699"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1776"/>
            <end position="1960"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1986"/>
            <end position="2013"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="2102"/>
            <end position="2192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="2294"/>
            <end position="2369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="2439"/>
            <end position="2498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1156"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="2082"/>
            <end position="2093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="2204"/>
            <end position="2214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="2518"/>
            <end position="2528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI68958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2536" mass="285685" checksum="933E4A3CF978F82E" modified="2025-10-08" version="1">MAEGDYEFRPKPAVLSRVELDELLRIAPPAEAIGRGFTLQEADVDRVISAARELEGAQVVADPNLPPQQTIETLLKVVRVLQTAIDVQFSENEELTADFNELQNNELRRLREENNRLREMRDQEEATADVGELRRRNEDLLDQLDELEQLAEQLRESEKEALARRDEVEESIKERDKELLKLQTRMADVEKRYERARDDYNDLKERYRTLSQEHDRVVSEARTQDTKQQSEWKIERLTKDNRALEVANTELRKDLQAVKEENLEVSEKIIELNTQHQELLSKNLVLETRIEEMASDKENLLSIQDELRTELQDKMTLLDEFEDKFNRQYRSWEDEKAGLVAQIEALRRESKSRRVVGKDGETVNAEPGSVSVVEVAELRQELNDVREDKILLLEAYEQLEEDVAKEVDRALSRQQEEMDRLKRRVDFLQRKLEEQDEDAEDAKDLMMALEEELKDAHERNKLYEQGLYGLPQAVEEIRQLRADLTKANKRMENLVQQINKQAAKVEDLYDENAMLRKQANLGDTDKVDIRDIKMQKEATIAQLRALNALLERQVADLEEERRKLRMEMKFRAKYHGQHALEMGLTNDKLLLLEEYADKLKNNRVEEARVVDQLQQRINFLEVRLAEVMAYADIPPSMRPALSEFDPTGLGTFQEAVQAAVAEVVQVQPSAMAALPTIKAKEVEAVRKVLSGCMRRLKQMSKSLLEQRNISDRDYQRFVDVILGELVDADKVLGALLKEPTALVTQPSAATGAEPGFVMPSPRSPAGSRPLTPLGSFAPTSGRTGVSVNPHGAAGPAVIQLLPGDAGYLADAVAEELRERLRGLAKQVADLQADVAQKDVRITQLVGQKADLERRIGLNMGDRKSEYVTIAEYEDVQLEVQALKDQLISVLEELSSREREAADLHDASLRYHSKMQMFSDQVKLLYREYAGAQAGWKIEKLTLEKKSQKAHADANAYKIATRELQMALDTLSKSRDLTQVEKEYLDTVRRMAVVQIKQAKLARELEASVSGEKALVARVTELEEEVRDVSSTARGRIRWLDSASEQSKRRLEQLYRELEISVPLTVYHSLVGKHTRLQHEYRKLLEEQAEAVVGAGQEEVFRLRDDLNEMLKRYDQAASQAAELRARLHQAEQFVKAAQEAATQHQSGDTKLKSGKQDAQSKPTSSTLAGSQKLAAPPIVLDNALGQELVAARVQEEAALRRVELVEKERDRVQHSLVDMQGYGKELEKRLAQSAAELELARQTASGLERRLVGAKTKDEVDEMVRRMHAAEAAAAEVQKSHSDLVYKAEEAERRAEAATRDRLRFLAEITNLKAALRDMSGRSDKAALIGKLHLELDHSRTKETLARNALNRSELQRIELEKTIRRQKQELANLTGRVVAHQDHAAWSDRQRHEAQAQLQVSLAGRTETWKARLWTRKLEQLKQRNESLADGLELARKRILKIEDARQEAELKLDFKEDLASWAHRSANDLTREVARLGDQLLQLRLEKGKLTREDMLLKEKVHYTERVNAELHDLLEKYEAEYFQAQSQLEADRAAAEMRAVGLQEEVEKLQERINSLLATKDLNDAATDGVNGAGGVTGLRGSRTAGHAFTELSDAAAGVTTFRKTLAERDEIIGLIEALKVAREEAEHLRTDRARLQGDYDATLVELEDVRRHHHDALDRLTQQSDALLYALNQFKSKGADATVFDQMKAVAEATINELKQRIKDRDSLIAELRKQLKDEGAQMLARHNADRAEIERLNEKLFERNDASIQDLKCVLDRLPLKQGAQQVPEEQLKLREALERAQDMITVLKNRLEQKDAAIDMLKLSYEQELQQLRDLLAAARSEAESRAAAAAKAARDEGPTKAHLQKLNTQLKTKDEMLRQLRAAIKALEAKLTQVMKEHADEKMQAASWHAQERMQEQLDRLTQERDELTRRLALAEDNVAAATGIDSEVQEQLAMLRRRIQEEIDKRVIIERQLVTVREELQKFKNAREAVVDLTTGMSIEAKRHIEDLQKRINVLEKQNAVLKRKAQEAPFGGPGEETNQAGAGVGEAGGDDFDGPGGGKRQPARGSSSRRGAPTRRDNDAGGGGGEDQTAAAVEHDPEAEERRERALLQWEEGKKLNGKIDSLRKQLAAKTNEVVTLQKELERRTVQAASVQSEADKQAAAIRDLTEKLRRAQDAPRTDRAKLQEYMERCTSLEETRDAMSVELTRLKLQLQAAAGSDSGGASTSVAPPASPGAAQLRRQEDVLELRLQRDQLQLQVKRLKERLAILGTAPDGATGSVTTRRAGSAGARLGVTGTGSSGITSGREAELLSTITNLKAALEKATANSTPTTKYMQEVNRRKDAARDAEAARAELERLKQQLTASSRMVAELQSANAELRRQLRASPGLAAAAAAGVGVAELGLQLSNLEMLLGQRDEEVAALRGALAQRDAELEALRGPDGTVGVASGTEVSSLRRQLRELEAENEDLRNELNAFDPSFWDEVMEMKQQHAELSRMVERYEELVIELSERLGIPPQLQRRGRATTGRTRGRSDAADDRPRPSPRRGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH15</accession>
    <name>A0ABQ5SH15_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012907</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1008" mass="109443" checksum="31395CD0D7338AC7" modified="2025-10-08" version="1">MVSGSVLPPVNEALSLLDVSFVNNEWHGTPATIRRSIQILVDHISVQNVQLSRVEDILNSFGSPSQQGGTVWSKLAAKADLNRLERVEAQVDGLLNDCAQLRTQQSAAVHAHDDVVSRMELLKDQVTSMEARLGLPYVPDTASGLDQYGGTRRLNTGLGSSSPSKGGLTTSVPTGSVLDRILRLERRTDALAEMNAKSEDDAALLLAKLREMEPRLEVQINHLRGDIKQALALAAASTRLEADAESTSRIKEAEKRMLARIQSVESSLEGKVSALSAQTGITLSSELDKRVNSVYDHIDAQVQRLCGDLERLSRDKVDLTSLEHYSQRISSLFEGVAQGLADEVATLVTQLRAEVAGVTRSAEEARVKDKMLQDNLKAEMEGIRQTSGQVKEAVSAMQEALAAVQNPRPLEETRAVVRQVMLDTEQLQKAVTGLNTAMSSTSNALELQSHKLSQLSHKISGVQHDVSAVKDTLYGGVASPGGNAVEQPAPAGASLLSKLTSLERTIADMTASLHSKTDDTVTREMERRVAALVRQQDITAEGLKNLSATAFKRADDHASAIQRLTLAVSGNLEDRPTTTVVKHLLETSLLEVRERCDQALAPLWDAVRILQSGLRDAAGEVKALSANVSHVQQAQSDLRSKASTDRASLLTALRKAIDDELGSLRTQLDSRLDSMASGVRQVVEQLEVHEKLQDRLTQLTTAIEEQLATQVAAWRTGNQQLRTELCSRADAAAVGLGARLDALEVVSCKHADELKVLSETSVSKPNEVIVREIASSIASTVSSSLVKQELFDFREKELAMLQEWLEEQKRNRDNIATQNNVAAALDSASRHIRQEMESITTARIEELRRAFASLRGEIDGRVRATTSRMEVAELRIQDLEAATAGTLNRKDVEEILTSSLSQGVGHMGLETKVRQMMDEIADHASTLKHFRSEAVLRSEFTAELARKVDLATYLAQGSARAAAANAGALVGANLSPQRRRSVDAGERFAHGVAAVARSMDRLNLGTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH74</accession>
    <name>A0ABQ5SH74_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013399</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45926">
        <property type="entry name" value="OSJNBA0053K19.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="6">
        <location>
            <begin position="98"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="7">
        <location>
            <begin position="242"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="337"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="385"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="541" mass="58266" checksum="AE78FFBA2B6B1A7B" modified="2025-10-08" version="1">MASSEKVLDLGDVDKLSRQQRVGRYKVWLLQKRLAELSAQVEAAVLEARQLIAWKQSYQPLTVAELTDEDKRMLDYNHRVRLAEVVQKHCSTALKSITQHKWAFPFNNPVDTSRFPDYPKVISTPMDFSTIKARQDNGFYRDPKDWWSDVMLVFSNAKRYNAPGSDCYLMAQMLQEVSEEKYDKVIAPRLAEEAAVTSREEVHQKKKRAELFNLQINEAMDAQCAVLFHLMAELHAAIREAKSLASSMCEPLTLEEKQALAATIQGLPTAQLESIVAFVASRHPPSVSTSEVQPGAAGGAPSRKVQLNLGRYDPLLLRQLQHLVSTCTAARQHNQNQLPGLLQPPQRPVASKAPVPRPAPISATTSPGSGTAQPVSGASAGIGGMAAPQQQPQGVGAPGAEAAVVQQRQQQQPAEQQPATLPTDGAPGQQQSRSQVSAPPVGSTQRNISDVVTNATASGISARAGIKWPGLAVGAGVRPRARVLLSLNLDGAASAGEMLAACPLPQPVLQQQFQSGRTRLTGLKRVASQALVGGCGGPLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHZ6</accession>
    <name>A0ABQ5SHZ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-3-O-acyl-N-acetylglucosamine deacetylase</fullName>
            <ecNumber evidence="4">3.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_014139</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that in bacteria anchors the lipopolysaccharide to the outer membrane of the cell. Lipid A-like molecules in plants may serve as structural components of the outer membranes of mitochondria and/or chloroplasts, or may be involved in signal transduction or plant defense responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine + H2O = a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate</text>
            <dbReference type="Rhea" id="RHEA:67816"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:137740"/>
            <dbReference type="ChEBI" id="CHEBI:173225"/>
            <dbReference type="EC" id="3.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 2/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LpxC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.108" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.20">
        <property type="entry name" value="lpxc deacetylase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1700.10">
        <property type="entry name" value="lpxc deacetylase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004463">
        <property type="entry name" value="UDP-acyl_GlcNac_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011334">
        <property type="entry name" value="UDP-acyl_GlcNac_deAcase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015870">
        <property type="entry name" value="UDP-acyl_N-AcGlcN_deAcase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33694">
        <property type="entry name" value="UDP-3-O-ACYL-N-ACETYLGLUCOSAMINE DEACETYLASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33694:SF1">
        <property type="entry name" value="UDP-3-O-ACYL-N-ACETYLGLUCOSAMINE DEACETYLASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03331">
        <property type="entry name" value="LpxC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="324"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="559"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="559"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI69505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="630" mass="69806" checksum="876C611976D88B7D" modified="2025-10-08" version="1">MAHQFLRRPDLRCRASAAGPCPNQVVMLRRMCSRMPSTVRSPSLRRPPQSTFNPRLARSEPCRAAAESDVADFLERPRSRLLLDTSPEIIDGPDAPELPMPSEYQQTLWSSFTLAGIGLHTGEYACVRIRPAFAGEGRYFVRVPEGTNASRAVAGADMDLQEELVEDIATEQALLNDPEYREELVARYQEYVDAQEEDGFGGSFVDYLQAQEYTNLVQRLEEEGPPKFKFSRPEEPQPRSEDELYVPAASAYIYEANPLTTTLSRGDFQVGGVESLLSALEACGVDNARIEIEGGSELPVLDGSAEGWTDEICRSGLRYAPSRPDLTIKLPPGAFDPPEASTATGEEGEQADEGRQGDGDSTRDQAKPVPKLVLRPQSVVSVIRGSSFVTLYPEDTFRITTGLDRHREAPVIGKQWLSWCMFEDVHYKHFLSDARGFVSSPEQAMGMREAGWLMGGTEGVMLIALDDRWYDPEIVRHDDEPVRRQMQDLIGDLSLLARDGHSGLPCGHIVSYDTDHELNAAFVEKLLESTTEEDWVPIRNVAGPSVPVELSDLMSDVIMDPEEDGANDGEGEAEGEEDEQEGMYDEGADELGEEDEEDEFEEENEEGVVDVEASDVENPRKGMGGSKSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIH6</accession>
    <name>A0ABQ5SIH6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Patatin</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014450</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="3">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07224">
        <property type="entry name" value="Pat_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033562">
        <property type="entry name" value="PLPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406:SF7">
        <property type="entry name" value="PATATIN-LIKE PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2 3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="132"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="390"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="166"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="284"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="462" mass="50950" checksum="32AF24BA6252FEA9" modified="2025-10-08" version="1">MQGTARGPSSYRRSMSWHRAALRGSFLGTTAIKQYRNFMNENLSLKHSTLGPRVFWVVPHSDPCSSGRSWSTISGLPPNFCTPLQSYDAETTRSSIGSRIDDQGVGVEEKERRARSRLEQVRRGLESGTLGFGFSAGGFLYPYHLGVLWELHELDILKDYKVQMAGASAGSLAVATYNCGLDPEKATQAMHAFADNCRSKGTRYRLGGLLRDFLHAYLPDDAHERCRGNTHVALTRLFPVVGSEMISEFESKDDFINALLTSCHIPFYFNGSWMTEFRGKFYMDGGVAAFIPRPPTEYVVKVCCFPVNEVLATVQDRVAQYERVASLLDVSISPDAYESWPFNYAKMVTWALVPADDDMLRYMINKGRRDARAWAQRMQLVPHDAAANLTGGEGAGEIGGGAERAAERVRQAQSQIEAAAAGLRNDGDGTGPREDSEALSVAQVTRGGEQGRVSRSGSERTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIM7</accession>
    <name>A0ABQ5SIM7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013997</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="244" mass="23686" checksum="8EF9E6262AF7EDAF" modified="2025-10-08" version="1">MVNVESIAASANASSNASANASANASANANSNASANASANASANASANASANASANASANANSNASANANSNASANASANASANTSANASANASANASANANSNANSNASANVAPLDLVYAKVVPVDAAGTGTNASSGSAVNEGYSVSEHGGLAVVLAGCPELVDAVLSGGPVARLVIRSRALGGVIAASPALARALCDSDLGRLEFSELIATRPALASVSDLGLASHVHCWRCCRRCQRWFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIV9</accession>
    <name>A0ABQ5SIV9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014027</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13365">
        <property type="entry name" value="PH_PLC_plant-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013591">
        <property type="entry name" value="Brevis_radix_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF360">
        <property type="entry name" value="ULTRAVIOLET-B RECEPTOR UVR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08381">
        <property type="entry name" value="BRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51514">
        <property type="entry name" value="BRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="312"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="364"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="419"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="617"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="749"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="domain" description="BRX" evidence="5">
        <location>
            <begin position="2048"/>
            <end position="2106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="815"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="902"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="937"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="991"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1078"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1222"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1311"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1435"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1622"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1663"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1733"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1798"/>
            <end position="1823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1852"/>
            <end position="1931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1998"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2242"/>
            <end position="2286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2312"/>
            <end position="2335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1512"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="944"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="991"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1016"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1141"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1230"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1318"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1354"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1693"/>
            <end position="1702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1734"/>
            <end position="1744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1798"/>
            <end position="1815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1875"/>
            <end position="1903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1916"/>
            <end position="1931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="2013"/>
            <end position="2024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2266"/>
            <end position="2281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2335" mass="240336" checksum="4E09171D99057E52" modified="2025-10-08" version="1">MIQEMSVDILPCPTYEGGSVFLNGFSDGPKPYEEVLTFLRKGTDMLKYGRLGAPKSHPFRLSNDMTELQWESKKGQVRRIPLKCICRLEQGQNSSVFRKYPKPESQHVSFSIHYTDESKRSRTLDVICNSKHDYEMWYWGVQIIMHYPPTTFYSTPPQALPAARTHALSSTFSAPAVTQHRATPQPAVVNNLLASVRTLPTAGDRRPKATPKTKPMAASLDLMKSMRSSGAAMGISQTDTRAQMGDLYAWGSLMVVEADEQSSSPGNPSASHESLRVVMQSNRPNLVGEANCVDVARVACAPRHAALITRNGELYTWGLGRDGNLGHGWCGNVPKPRLLTRMGGRGVRTVSCGEGASAAISAGQRLFMWGKGTAGQLGNGYAFPCAEPAPVVFPGLREDVKVLAVSCGPYHTAAITEGGLLYTWGNGLFGRLGHGSHKSEYRPRRVMELEDKCIIDVSCGYWHTAAVARPRSSTRSRPASLTPSMEFVTPASTPGAQPHPFRHLPTTASVPNMSTAGDGVEDGSRGELSLRIRSRTADMSSSGSDLQGAVAAAAASVNAARAHVRGSYGGGDPSSSNAGPSVGYLRDEMTSCSVASFDKLPDVASEDFSESLPGAAGILFTWGGDSSWMDSGKRDKHFGCLGLGDDTEGRLAPTRVRGDMDKHGVVQVVCGWSMTIALSADGRVYQMGSTGAHTTDKNCTWEGAKIPTRVDGNLFGMFVEEVACGMRHVVAVASKVQANGQIPDDQRRVRLLAWGCGAEGQLGLWGMGTSTDASVAVRAAQTDHSLPQIVGCLDGRRVLHIAAGGNLTMAVMEHDSRTRRSNPGPSAMPAPTNHFGSSTTSTPSGGGAHSRTLSVAQYQSVVGGFATGVRDASSAAAFQISQSLPITVAAAGLQSLQQMVSGGVGSRSKGGTPTAPPDVSFMPRRRPSRVVGIPQLTFGQPRSDHHHHRHRSDLRRSTTRLLTSYQGFSGQVVQKALSSGGQEGKMLDATASASSGIVSQRSSPPLGQPSEDADSRSASPTSTSLFEISNVGGDFQPSLHVRSAVLETRARSNSVGSAVTGASFATVAAAAVASFARPLQRRGSSGNAADIQSLGDNSTGDTPETDTDSYAHGGRSLDMVPEQSEESPGSPSPISRHSHHAMTTAHPHPHHPMAATIAGAVAGVGGVHPHSHPYSMEGREMSTMAVQVKPPAAVKPRLSRLAQQGPAGAAGTAVAATSTALPDGSIGETARSSVSTMDSSYTAEGSASSELSPSGIITASPETPAGEMDSVHPAVAGIPTLPLPFTLPEAQLPASPPGPQQHLPFASHLMALTPPSPQQHQQQQPTTIQASPLPPAGPPSRHGSLGHVLSPQESLARAQAAAGGAVTPASGSSGRNRDTGVNMWVQAPLAAAGGAAGHSSFRSEGTMRAYGMAPDSVAPSTTVGGHAAMNMPVGAGHRSAGSRGHSRHASYDSVDNTSRDADGIGHHGSNVAVGVGGGGGGGGGFDPRAAVFPAYGVASTSGPLRSGTDPLTANLLQQNEQLQKELEALRQQVALLTHAAGLVPAGASGTGMLPAGGHPYSASSSIPPPAQQQQISAAAAGAAAVAGVAAVAVHNSPLAMAGGGGLQPYPGGAGPLPTSFLNDSANGHGAGNSGQSAFSPQGPVRPAMLHHAHSGGVNTPTVGVSSPGHSFAAGQGTSRSPPTIDRQASVASAPPPVSVPPPSHRHKRSSSIDVSSLIATRDGIGPAKEYLAPVPQPAPAPSLPPNHAGERMPSMGWESTAVGSHSAPPALESLRQAQAGVSLPQAALDELAEEDCLEEGYEGEEDDQEEGEPAVDESLGPSASIDGLGSVTLAVALPMATQGSLHRQLYVAQQQRPPQQQPQLPPQQQLQPQQGPTNRPSASNPGHSYTSNNEDPPSDSVTSLKDGPVRPISENSYRSSESGDGSSYTASAVEGPASALAFPSAVQEADVSLTAAAASAAAGGGGGAGPIWRTRRNAFVEWVNTMHHEMTEEMQNGGGGAGGNVGAVPGHGTIAGGSGAGAAPGGEQLFPAGSSVPLAPVAETLPPEGMQFQYAPGVFITIKPPAQKGGRIQLVKVRFNRKMFTEPSQGQAWWSLHRANLSDLYHVTFDMVNKSAAPLPPGLPPLAPGPHQPVPTATMHVRGMSTDTQGTSAGSTAASASSSLYFSVPAGNSPVQGFAATGNGGCAGSGVPATVTAAVPFSDGRTMWISGASVLAGSASGGGDLHEIWTDVSYDDTAMMTSPAPQPSVGVMGPTMPPRHHRSRSGSRTASGGSSHASASRLGQAQCPESPVLALQQVGTSAAYPGPGAMVAAAAQPHHPSHQRTRSGGGTPNMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJD1</accession>
    <name>A0ABQ5SJD1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor SLU7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_014424</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLU7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021715">
        <property type="entry name" value="Slu7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039974">
        <property type="entry name" value="Splicing_factor_SLU7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942">
        <property type="entry name" value="STEP II SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11708">
        <property type="entry name" value="Slu7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor SLU7" evidence="10">
        <location>
            <begin position="133"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="149"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI69746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="521" mass="59044" checksum="089230247DF050EE" modified="2025-10-08" version="1">MAFKTGDEWRRQKELDEARKAGLAPAEVDENGREINPHIPQYMTTAPWYLNSDKPTLKHQRNWNDKGKDGTQWYDRGAKVFQATKYRKGACENCGAMTHKTKDCLERPRSKGAKWTNKNIAPDEKVEDLKLTGFESKRDRWNGYDSKEYARVVDRFEQLEELRKEIKKKEQLEMIHKKDEAAEVTVDVETSGDGAIDEDETKIKEEEEAGFGEVKKRVRTTAGGSTGSVRNLRIREDIAKYLLNLDVNSAYYDPKSRSMREDPQPDKPASEKLFHGDNFVRNGGEYAAWQSLTLHSITAHDKGLDVHMQANPSLAEMLYKQFKEKKDQLEDKSKEDVVKKYGSAAAPLPDEVKVLTGSERYVEYDRAGRVIKGVEVKAKSRYEEDVMINNHTCVWGSWWRDGTWGYACCHSNVKNSYCTGKAGVMAAAQEVEDGPDLELDPNKVDVALRKLEERERAAVEGDRSKRKYNSLEAGAEDVTPEEMEAYYLKKARGDDPLVAMTQTQKGGVTAGVTGTPGYDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJD4</accession>
    <name>A0ABQ5SJD4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="2">5.4.99.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_013885</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02573">
        <property type="entry name" value="PseudoU_synth_EcTruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00431">
        <property type="entry name" value="TruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767:SF2">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE TRUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="TRNA-PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase II N-terminal" evidence="5">
        <location>
            <begin position="130"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA pseudouridylate synthase B C-terminal" evidence="6">
        <location>
            <begin position="279"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="335" mass="36996" checksum="5900471C3EBF579F" modified="2025-10-08" version="1">MKVQVRGMDRVVISRRPLGCLLRRPPSKSRIKVTVSEFSSRISGALITSALADQAVDARDRTFDASTSQGVDPDSTFEAARKPKRISPTRLIPGPIVDVDVLQNGVILVDKPLGWTSFDVCGKIRNMLKFTGVKKVGHAGTLDPNATGLLIVCTGRGTKFCDDFMAQAKEYSGTMRLGEGTASYDAECEVSERRPWEHVTDLDLQAVSERFLGDLMQVPPMFSAIKVSGQKMYQLAREGQSVELPPRPVTISSLRLWRSETSPQDVHFYVACSKGTYIRSLAYDIGRALGTAAHLVALRREAVGPYRVGDAWDLRQLIDQLEQQKRASLLSQQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJU0</accession>
    <name>A0ABQ5SJU0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BBSome complex member BBS5 PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_015091</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BBS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006606">
        <property type="entry name" value="BBL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030804">
        <property type="entry name" value="BBS5/fem-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014003">
        <property type="entry name" value="BBS5_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21351:SF0">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21351">
        <property type="entry name" value="BARDET-BIEDL SYNDROME PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07289">
        <property type="entry name" value="BBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010072">
        <property type="entry name" value="DUF1448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00683">
        <property type="entry name" value="DM16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BBSome complex member BBS5 PH" evidence="10">
        <location>
            <begin position="37"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="BBSome complex member BBS5 PH" evidence="10">
        <location>
            <begin position="167"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI70245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="351" mass="39475" checksum="15A18842E34B5377" modified="2025-10-08" version="1">MADILFGTSSRDSQFCWQDREIRFDCNSKDITPRPCEEVIAVLESVEDTKGNSGDLGELHITTLRLMWICKKNRRTNISIGHNCIMSVNVRSATSRLKGQTQSLYILTKYHNQRFEFIFTATADPADTLFSSVYGVFKAYDSSRLYRDLKLRGALIVDRELKLLPLEQTYSRVQGVYNLSAEQGNLGAFFISNVRVVWYAQMLENFNVSIPYLQIRSVKLRDSKFGQALVIETTPCSGGYVLGFKVDPKETLDYVYKEISSLWQVYATNPIFGVEYRPDPSGIAGGTGIGISPSEQDVEIVEPTDRGDALAQYYADATKGVDRDPVFCAELGLAIEQLKEGLTIEQLWSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK41</accession>
    <name>A0ABQ5SK41_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein CLP1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_014420</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Clp1 family. Clp1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1030">
        <property type="entry name" value="Clp1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.330">
        <property type="entry name" value="Pre-mRNA cleavage complex subunit Clp1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03035">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028606">
        <property type="entry name" value="Clp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010655">
        <property type="entry name" value="Clp1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038238">
        <property type="entry name" value="Clp1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032324">
        <property type="entry name" value="Clp1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038239">
        <property type="entry name" value="Clp1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF6">
        <property type="entry name" value="POLYRIBONUCLEOTIDE 5'-HYDROXYL-KINASE CLP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06807">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16573">
        <property type="entry name" value="CLP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clp1 N-terminal" evidence="8">
        <location>
            <begin position="15"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 P-loop" evidence="9">
        <location>
            <begin position="123"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 C-terminal" evidence="7">
        <location>
            <begin position="319"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="126"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI69742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="431" mass="46395" checksum="9A6AF287C9BB00A6" modified="2025-10-08" version="1">MVAQEASNTNTCFNLTARQELRLECSNGRPVSIRLEEGIAEVFGTELNRGRAVSVTGQKVAVFTWFGCRVSVQGEPTVMYVADETPMASYLNVHQTLDQRREEAKRAGQGSAGSNGPVVVVVGPTDSGKSTLCRLLGNWAVRSGWQPTFVDLDVGQGTITVPGCLSAVPLEQPVDIEEGFIADLPLVFFFGHNSPGENPALFKSLADKLAALLQRRAAAADPRVAASGLIINTFGWVDGLGYELQKHIIQAFQCDVVLVVEQDRLYATLQQDLKVTCPRTNILKLAKSGGAVKREGDERRGAREQRIREYFYGTARTPLQPATQTIRATDLAVYRMGTGPRAPSTTLPVGSASLADPLRLQNLPPSLEQLQCLLAVSHAATPDQILNANVAGFVLVKDVDTARGTLTITAPAPGPLPGRYLIMGSLRSSME</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKL5</accession>
    <name>A0ABQ5SKL5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_015290</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07829">
        <property type="entry name" value="STKc_CDK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF548">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE A-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="110"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI70334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="401" mass="44748" checksum="8A7FF6C31DAA9B07" modified="2025-10-08" version="1">MAHGDIGCDPRFSMSGGQMQAADGCGSTSSAGASEQYPPITHLLRHLSHFKALAAERELANTAIAPLVRKLPFDQELIKLLEKFGWHPAHGFTTTLDLAGLKGALQKYKYIKMGQLGSGSYGVVHKAVNRETNELLAIKKVAHSPEYGLSDSTIREISTLRELTHENIVRLKDIIATVNGQHVHLVLEFLDCDLRQYLDTYPQASEIGRIKSIVLQILRGIRHAHMNSIMHRDLKPQNVLIGVAKNQVKITDFGLARCFLPNTDRAYTERVVTLYYRAPELLLGAPTYTSAVDLWSVGCIMAEMINFEPIFKADTEIGLLFRIFEKLGTPNKEVWKELPGLAHFSDQFPSFPAKPIQELVPRLANDAAGLDLLSRLLTYDPSFRITARQALEHPWFQGVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL33</accession>
    <name>A0ABQ5SL33_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012663">
        <property type="entry name" value="CHP02450_Tryp"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02450">
        <property type="entry name" value="TIGR02450 family Trp-rich protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09493">
        <property type="entry name" value="DUF2389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="321" mass="33431" checksum="383C3DD1F531815E" modified="2025-10-08" version="1">MTTWTLHQCHHICKYHHRHDRPADKSSLVPSLNRTTRPVEAPALTRSRNILDEDRNTICRSSFQFIRRQHLEPEPELIPQRCSQCGGTGKCTCSDCAGRGRLSRAGYNKRNRVDMTRVVGSKWTAMQETMGWRHFRVIQCRKGASGVAFVHMQASCDPATQLWINASNLKDRDLWAAGWLQKSEMEDPTAAMAGGVPCRACSGTGAIPCPVCDATGAVLLPPSGLGGPAGVPAGSATAAAGAVEASSTSTGFGGEASFLHRSGSSLGHTLSGRTVMGTGNAAGAVAGTGSGPGPAAGDAAGVAGGDSISSATGREDGAFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLB0</accession>
    <name>A0ABQ5SLB0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_015132</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15543">
        <property type="entry name" value="PHD_RSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="13">
        <location>
            <begin position="905"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="1221"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI70411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1345" mass="145478" checksum="0FDD73CCF8A2B49A" modified="2025-10-08" version="1">MDGPVLRRVHPDRPLILHTDFSGVGISGILGQLDDAGREYLCAAVSRSLNVHERRYDSYKGELLAVVWASKLFYPYLATRHFTVVTDHAPLLWLMSQQDGLPAQYARWALQLQAFAFDVAHRPGKLHQNADALSRHPRDSAHDGSGARVDEDGDPLRSPPVLVPYPGVQWPLYAFSRALAAAGAPAAVAAHLTVVAACSVALAQRFLDGQDLTNPASGLAAAPDAVGPLAGLAHFAGPVPRARAATWCVHAVCLVASTGLPVTAASELDYDNELETLAAGQLAAGGLEAPGAGQPALGELETSVRGVLPSAEPHVPCSLSGPPVTGAVTLQTAALLIRRKAAALRRLRPAKALPLRLRVGTGEVLGVNTALVQHVFFPAAAGGEGICVLELPGGLASGLLACLGLGFRVQRHVHLSCDARERLAVTRLVPIWALHFAGQLAAGAWDPQLVPAFSIPADLLYWPAAEALGFGDESVWIVVGSWAGNSVWSAAQLVGFLGGFQQFRRERRLSPPAFLLEGPFVNVNAAPDAGWLFGLPVLLDAAVTGAPVHRLSCLYTNLANAVHFQHCVTELRPPDGRDLAALLPAGRVPAAACTVLAPPYVACDLPGRPWVALPPPAVTGTPLRAGPIRAPCQVTAADYTAAAGFWLLTSRDFMLELELELLERSSPPPVFQVALSLCMVLHRTFITPHDMQYPATKHLTALPQGGGSDLTPLAPVAALATVGSAARLAAGMTHNSAMVDADNLLTPWACALLSQRAPGLTVVMATAAVAAAAEVQENALAEGQDPDVWRDASTLAFLRGAPAGVMEEDGRPARRVLRRAALYQWAGDQLLRALSDGSTRVCPPPEDREAIVRRVHAVAHLGIRRTLALVQLGYWWYRMRRTVQEVVRGCKLCDMTNSIGTARPVQLQPLAIRGMFYRWGVDLAGPLPPTEPFGFTFVMVCIEHFTKHAEFYPLRSKQPEETSRGLLELITHFSAPAEVLTDRGGEFQGAFHQFLHQCFVDHRFTSASHPQADGAAERMVRVVKAALRKYCADVKRSDTWDQHLPWLALAYRCSPQASTRYSPYELMYGVKPIVPPAVRERMAEPLGFTGKGSEESYVAALHERAALLRRHMPIAAANLLIAQHRDTQRYAHVRSGRYRPPSLELAPSDYVYVRRTAVHNTLQLPVHDEVLRIERVGPMGVVVLVGKNGKRMRRRMEQLRPCHLVDIDPIVDPRLQRPSADLACEVCHSPHDEAQMLLCDACGTGWHLQCLSPPLSGVPPGQWVCPECVRLKREAPTGPEMDLPEAEAVLFPNTASRRRDAEAAALNGKRVRRVVHASQGGRRQKVVTLGTLRFVERWRGHTTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLB5</accession>
    <name>A0ABQ5SLB5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015004</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MlaE permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003453">
        <property type="entry name" value="ABC_MlaE_roteobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030802">
        <property type="entry name" value="Permease_MalE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00056">
        <property type="entry name" value="MlaE family lipid ABC transporter permease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30188">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30188:SF4">
        <property type="entry name" value="PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02405">
        <property type="entry name" value="MlaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="344" mass="36192" checksum="85BCF6C03588154A" modified="2025-10-08" version="1">MVHSGRLGLAAGPTAPHVAILPPPVLRAAAIGPRWAAFGPVKRARINNTHAANSTLDAIAPEPEERESTPISQAANAPGILKALDQHKAPKWLFRTVACLVLGGQVIMRIVKGNIHWRNTAEQLRLVGPASLGVSLLTAGFVGMVFTIQFVREFAKLGLTRSVGGVLALALSRELTPVVTAIILAGRVGSAFAAELGTMQVSEQMDSLRVLFTDPVDYLVTPRVLASMIAGPILNVLCFCMGMGAAVLLADLVYNVPANVIVDSARRALTSFDVVTSMVKSWVFGTIVATISCAWGFTTSGGAKGVGESTTSAVVISLVTIFVADFFLSFIFFQGQGTALKQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLP5</accession>
    <name>A0ABQ5SLP5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acid phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF207">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID PHOSPHATASE TYPE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00778">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="594" mass="64773" checksum="6558AB0C784523D7" modified="2025-10-08" version="1">MRGHQLPIGAIAFASGFLNVVNCGNDIDRANDRGRCPFPLPWTIPWLPSVACRADASGYDSMEPTLAEAIPATGELRLPVSGGRDPDDNTDTVTGVFHKLNPQHAKLLLVQVVFRHGARTTLSNRSELWRGQQWDVCGEAYQAAAVRLFSTAGVENPVSKHDLRQRSVVLEGGCRKGELTLLGQSQALDLGRWLRSNYVARDGFLPPTYQDGVVEARTTNISRTIATLRGVLTGLYPDLSKQQHPEHQQQLQQRQQEGQVLQPGSQLLLQPQLQEQSQLQEQSPRVPHVTTSADLDEILYADTRACPHLGSFQAVSRELGRAAVRSDPDYEWARGELLGLLGMDPVSFDSNRWIFTDVHDVMTSLAAHGKTLPPGFEGHNRLLAVIDRLATKEFSAEVAPSMRNSHGLTILRLSMGRLLHILVENMQRGVVAAAAAAGGAVATSAGTGSSSAAAAADAARSPFVAAGSVDGKTQPKMYLYSGHDSTIMPLLSAMGLDIIKWPPYMSNLVFELWQLPSRRPGAEPRYVVRVLFNKEELSLPHCPPGFLPSLQTFQCEVVGPFQLSAQRHQELCRVKVEHDGAMPQPKAKAASDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SME2</accession>
    <name>A0ABQ5SME2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046106219" evidence="2">
        <location>
            <begin position="27"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="524" mass="55679" checksum="2DAC25EC5626D4EC" modified="2025-10-08" version="1" fragment="single">MHRCNMSSRQVYKFLLLFLPIITAAAQPVHDFVHGSTLSRNLWRQVWGPGSSALPTGITDVSSFDVSSALYFVRQLDFALQRVSPFTKGFWSEALTLARTFPPVADWTTARWKDGTLPRFMGSFATDNGLTDPRGVPIQRDVAATAAATADMIAGEGAVIAVAAADTIIGTLQASLRKIGGAAAGQIGSTAALIRHVVGDGKQYQHDITSAATHGQRQVRRVRRRGLQTATPFTEPVGTAAQIVAGIITGQVVDGAAKQLDGIRKPSSRIDPGDLSAAAAGNLAGGFRRQAKRIIREGRAGAGAGAGAGAGAVKKVLQPVLHALLQTGTGSVITDTLFSLFNRQHGARAGALDELLMLQQILLDYLYGSPLTTEIIHKLMYGLIDELAKADSRLDLEGVALDAITVLRTLLNSVLYEVMLDGSDLQEAFKLSDADRRVMRPFLGSLLRLLAIAEDALGALFEDYPDYKFSLPPSPSEIPPRPPVSEPPSPPTPSSSTQGPPSANKEPLSQSRSPPPPPSPRPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMS3</accession>
    <name>A0ABQ5SMS3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_016077</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="10">
        <location>
            <begin position="1975"/>
            <end position="2117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="464"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="526"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="716"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="949"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1034"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1200"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1293"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1733"/>
            <end position="1780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1903"/>
            <end position="1927"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1136"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="496"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="716"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="973"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1013"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1034"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1227"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1307"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1733"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1908"/>
            <end position="1918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI70974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2177" mass="231557" checksum="68FB11CACB89CDFB" modified="2025-10-08" version="1">MASLLPPTSVELLQSQFNSSPHAVFAVSLIDVQTTWGLRRHEMEVRHVGASIKGQRMLVIKWWNPAAAGILDAEAYSPRISIRAIMNITTRLLSVLRPDVSLALTACLDDLLAGQNLKQLPLNAYGIQVQPCLILEPCREAARTMPHRRLRPSSCAAGFLQRCFKPSVETFDGYGYDEGVGVKNTTKTISYHEEYFSAVPSATLLLTFPTITTLADLPIHHATAAPAAGASPPAPSPLVAFVKAATAPVVAAERAAMDVACLAGLPVCVTVFTVAGQIRHQNAASVQYYGNRSVVRVSGIGIDIGNSVDKPLAASHAPVAPGSVDAVFAMNNSHVSMAGAGTMDTMGGRSCCTPHAGRHGELAAAGGDPLQVLQMETCPYSLADASVLSQLFKFDRGKLEQMMTCVLEAGQVWEGIVRVPESLDPVRRSESSHKSRSLSLSIPKWTNTTCSGEVPQVNIAKESEAQSTMNTNNASVLKPQGSTPPQGLPSPVLPQKKTEQEQEQDEGRREGEVELELSANHVLIEASDTSRNLVPVSEPIPESGEAHSSSTTTETTKIMAVAAPASNRGDGDGDDCEDELYASGGADFDITLARTKSLVQMPSPECSASSTVHLPRALRRAAGDSICNGVSVGSSGVGTAILLGVKSQIPSARESPVISAAQVTPLLQISDTLGLTRRKSSWQLPNGEAIANAVQKLHFTSSATVASTAAGAGAAASPADAAAAPTSVTNTKLCESRRSPPGGPPGIRGDVPPSSPPRRPRRFARIAIPKPKKNQGATVHGYFPSAGSLPSISTHTAERLSCVDVSAAGNDSTGSSSSLQQRLLQPPKILQHLQQYYNLQGMRGLFRSGSLLSAHSRHRLLDSGSIHKGGEVAAAPEAAATRSSTRSRVTATVASGANTAIDCAEATAVNSEAMRGVSSLRLSAVFRSFSAAGLKLQAADVEFGVGRHVNTTSSGARYPDGQQRGGGERTLAGTVTSTRSTVPLTQMDESMEASGPTRMDQHHPPPLPPPPQQQQLLQQQQQLLAPLQQQQISGVQARQQQQQQRMRASAAVGGLRPHRKPESKRFISTDPLATSTWQVLFEPTNDSFTRTTLPDYCTVPSCVLNEIFLSDESLTDGTTLLSNKGYPEDRQQRQYMMRQQQQQQQQQQQQQQQQQQQQQQQQQQQYDAQEGGSLHEVGDMRKDANRGPCRVLERIRTVLQAASSSPSEGGEVRCGSTGGSEHAISRPHQQPEQQDQQELEQDHALWEAGSGTYPPCSASRCEQQEQQRQQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQPEDDASDRRRSDEDKPSDKLQPLEVVAIRRRANTHRLLTVPTTTNSSVLAAGLPQPQPLKPLSSSEGRVVVTAMPTDPENEKVVLQTASSRNHGMHNLYYTTRLHASSSAQRMQHQQQTSTADKTTTTATSFIPSQHSLRQQSSLDGPLDFSFTLRRPDGGGSIGSIGGGNYIAAARTSSIASTPSLASANLACSSSASPRVLQFANSLRGAHTSHCASSSAAATRNRSLAKTFFALAAAADGSPAVTAAAAASGGSIQRNNSPHVMSNGTFGRSVSCASARELKLSTSCVSNTDMLAMANRSTDAVLKRAFEARDGCEGSPAALTDTTLTKEVAPSACHKVDVAKTYMVTAAAGETMTTTALASINNNNNDAAGAASGGSCGGGELSEIFSVGDPLGLPPLMISQSSTATRALMLCTTTQDSLHAREDHEGNLSYGRRAENHNDIGPRRANAREGSSNHDGGDGNKQQALPSGYGIGVAGGGGNNEGELISSPTILTKQHGDSVAAATAGAAAAAAAGDDDMAARPPFLQGGGLPTGLGWHEVRATSIVSPTDGQRYVLLIQKDVTVKVEAERHIAKVSECEHRLLEQIFPRHVLAYMTEEGFSSEPPPPPPPPPPPEEDQSPELSSLVDIATAATAGHDHRQPSRPSALSLQAWRPYVRDCTRLATWHERVTVLFADIQGFTPMCKQLPPCVVMKFLNDLFVRFDAQLDMYGVYKVETIGDCYMVAGGLIREDHEGMATVQGGDEIDPLQADKVFTFAQAMLRAASHVMLPTTNQPVKVRVGIHSGPVVSGVVGTRMPRFCLFGDTINTASRMESTGTPGAVHVSADAYALLSDACCKQTGWSASGGIQVKGKGLMETYLWTPSSAGAAAAVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMV3</accession>
    <name>A0ABQ5SMV3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015754</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="305" mass="34214" checksum="058C5D1E00292C6B" modified="2025-10-08" version="1">MQIFVKTLTGKTITLEVESSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN71</accession>
    <name>A0ABQ5SN71_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="161" mass="17926" checksum="85D4902EC2558508" modified="2025-10-08" version="1" fragment="single">MAIQTRHVIILPDQLAYQLVVGCCQLCSSFHFLTDQLVVGCCQLRSIFCVFYVLMDHVFQTREPQGARQQDKADMHAYMAGFPGCCPIELVLSTQRNMVSTASILAVQVVQMALEDKVAAGAHIQELYKDDPDIGSMIIVGLINLDLAKFMPELPLPPVWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNA2</accession>
    <name>A0ABQ5SNA2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_016058</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF465">
        <property type="entry name" value="EGG PEPTIDE SPERACT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="7">
        <location>
            <begin position="137"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="7">
        <location>
            <begin position="275"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="70"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="404" mass="41600" checksum="CAA52DAEC088DA2D" modified="2025-10-08" version="1">MRSRIKMVGDCYFWIRIELGNVVVVSSVLYAELWPLFRCIRDLCLGKNGLFVLLRFRGFIVVQRRALPTPSAAGHPLAPPPSPPTYVPAGHPVAPSPSPLPATPPPPPMYGGSSPPTYPASSTPPPPGVDRLPPTDISLVMASNDTVVSSTPGMGLVKIRSRFGSNQTWSYLCGDTWSGWDASAADAACRQAGYPAGGEPLRIWSSNAQPTMFLGRLRCSGSETSLSACVATGAPEQLIDYWQLGVSGADAAGACDMLAGVRCRTGAAASAAVVLRVASGPSPSGTAVTGNYTGTAIRLEASVGSGPYGAVCLDGGFDNSSALVACRVLGYTSGIVYPPGAPTGTLALGVKDPTLDAQGSFLGTLSSVRCGGSEANITACASWRANVGTAVIPCRAAAWVLCNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNE8</accession>
    <name>A0ABQ5SNE8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37330">
        <property type="entry name" value="CONSERVED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37330:SF1">
        <property type="entry name" value="CONSERVED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="618"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1150"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="222"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="623"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1279" mass="136420" checksum="85EE0EBEB6DCDB23" modified="2025-10-08" version="1">MGCGASAARVPSVTASLDQGNRRDKSHPKNVVLQEGVASPAQRNSDAQPLELVASGAGAEKPEPAGSQSSGQQLGLGGAAEDGPNAQILQDLIPGQDLDSLPVEKSQTRCTCRAPAQADAGDTREDAQENREPTTSANFGAEAEAEDKSDQDTEPKSEQEPPEPGKGTGAGGETTVTEQPTATTTTITTITTTDVDYNCNNGGDSDRELAVPANSQQTLFRPPSSSPSSSPSTCSSPPAVTMAPSQPTAVQPASSPPSPPPPAPATPPMAPAKQLEQKVQKLPPQQTRMVNCAVSLQFLIDFCRTVPEDMPTWKVVTDVIVPATQQRQCRYVEMIEARHVGPCDYFISHRWATPFSHLVRYVRKHLVELGDVVEEGGAGIGGRSSAAAAVAAPAGVVLTKAESTKKWKSSTADDDEEAGPTRSADKVFVWCDIFAINQHPGQVQADDLAQLKDCVEASNQTLLCLDDKGLVLTRIWCLYEIWNTILAGGPPKLAVLGYDVNQDAFKEVYITLDVSEAQATVDSDRVRILADIAASTGLQELNLVIKQSLVESTQAEAENALKVMLPKAKEWKDYERVIQALNKHMQMLVALGRHQESRRYGYILQTVNTAWQKAQAGKPAATATGDSPASSPGAPSAGAGAATAVGDSARAVAGGAAANVGEGNLPEWHWSKYPRDAALRQVVNKAASAERHGRFETAMSMYRDAVSKAQQRLQSPRGGGPLAPLALDLAGCQLHLAVCLAAQGFPDQAVSEAGEAAVQAFKQHSGPRSLPFASAAHHVAVLSKNNWESKDLRALLLDAAYGIREAQLGPTHEATLETLREKADATVMGGDMAAGVQLYHKLAVRMSEGTDLAAKPPSSTSAAAAADAAAAILEISAKACEVWFLLATYISGMEGRSDEAIRICKALIPHKRRQHVRQLRDVVTDPNHKYFKDAVSELRRLGFSRAVVSSRPSSTASSVASSTSRRGVSGAIDVIDLEPDGLADPDDPRVDVLTHSSELNLWSTVLAKKGKLRDAVLLQWRMAEAFRNNEEMAKTETQLPTGKFTLLEAYERCLILDLSAPGVAKLAAEMLAERDGGPAGGAVTPLPTTLAPAAAPAAAAAPVYSGPSYMELIQQMTAKWSALDFKAAESICRQIVECQAAGHGPVSKEVVDARQQLAQLLNSAGRIEEALKEMRETLALHEKLLHGKDPTMNRTLQALADMVSESDRAEGEKLHRRAVDLLTKVYGPDHFEVGAAMMQLASFIMSDCERMEEGQELLQRGLAIHQKFQEQQAAMTKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNV4</accession>
    <name>A0ABQ5SNV4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleotide-diphospho-sugar transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015963</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052636">
        <property type="entry name" value="UDP-D-xylose:L-fucose_XylT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032:SF1">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045984523" description="Nucleotide-diphospho-sugar transferase domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="2">
        <location>
            <begin position="137"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="379" mass="42241" checksum="5A3512A03850EE0F" modified="2025-10-08" version="1">MKFISAALMLAMLGCYSWTAEAHGRKAIKPKFDPRFVNEFWNGGGARLPGREFVEAVQAASLTVTDGRGRQRRLVLLSLVSEFALSGSGLWELFLGNLRNITFSRREGSQDHLANHLVARLMTMPNATATHCNRLSNRFGARCVPMASPIFNADNYGYKTSAYYGLSFVKVLTILDALTIGVDAFFLDSDQVFFRNPLPYMLARDIDVLVTGDCGARDDETPSARFPKINSNIGLLYFRSTPMVTRAVNEWLFYLVRKARDRRPVLDQGTFVGAMETISTDVGAQRMSVAMLIPDFFPHRCMSPCGCDTEGLSPGQLGRKLYRTEGSACQPLFVRKWYHFHVPCNGDMNSKATTLQKLLRLYQGVVGPVNSLSDPFDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPN4</accession>
    <name>A0ABQ5SPN4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Obg-like GTPase YGR210-like G4 motif-containing domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016506</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046814939" description="Obg-like GTPase YGR210-like G4 motif-containing domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Obg-like GTPase YGR210-like G4 motif-containing" evidence="2">
        <location>
            <begin position="156"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="395" mass="43142" checksum="982A6E00EA0BE292" modified="2025-10-08" version="1" fragment="single">IASLILKCICHSLLVLLPRQLLQLPHSFFCGKNSPNRFRTRRRVCILARAYPLGLTPKPRPEGALYGRWVREELHRWVYDNVRAKWVYVLRKPSRLPELFSGYRASRALVAEVLSRVGVSVGALVVEGASLAHWTEAHLHRLVAHFLQARFPILLALNKADMASSPDHIARVRQVLPYEPAVPVSAATERWLCNQRRAGKVSYADAAGETAPTSDDPELVQQLNKVQSAVLNRYGSTGAALALTCAVAMRPPRAVFPVTDLESYTALPARGIASGAGSLLGSGLDAKARAAEGPQPGVLRDCLLLKPGSNVVDLFEVLKRPPYQLLEGDFIRAESSALTSAAAASATTIDGGGGSGGNPVPHQPWRVLKKDEVLDWDSCVARIMTNRRSHWQAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPY7</accession>
    <name>A0ABQ5SPY7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_017030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="11390" checksum="619A884AC20C5E10" modified="2025-10-08" version="1">MSHTSKIWDWLGLAEAADALHVTPTSSGDSSNRSSQDSSSCNEGGIRLSRPFPKDPSLRPQSRQFESGSGQNGEGGMQLGLPDSTKLGPSPPPAVERVKCIAREVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLK9</accession>
    <name>A0ABQ5RLK9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_000251</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ZNF598/HEL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16615">
        <property type="entry name" value="RING-HC_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057634">
        <property type="entry name" value="PAH_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041888">
        <property type="entry name" value="RING-HC_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056437">
        <property type="entry name" value="Znf-C2H2_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044288">
        <property type="entry name" value="ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938">
        <property type="entry name" value="ZINC FINGER PROTEIN 598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23202">
        <property type="entry name" value="PAH_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25447">
        <property type="entry name" value="RING_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23230">
        <property type="entry name" value="zf-C2H2_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="8"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="15">
        <location>
            <begin position="780"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="329"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="400"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="489"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="526"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="814"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI58515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="959" mass="102539" checksum="B115312F48CED3D4" modified="2025-10-08" version="1">MSMNDDLCLVCADPLEFTAFGSCGHKEVCSRCVARMRFVLKDRGCVFCRQENVQAYFTRFMGDYTARLAPEEFDQLQARADRRELFVLPEISGYFDDQKHFQEIRALCGFTHPILESGGTKSTPKFNSFTALKRHVEAAFSQFFCDVCLKGRKVFVSEQLLYSKDTLRRHQESGDESGPLADSGFKGHPLCKFCRTRFYDSNELYRHMEGSHEHCFLCRRAAPDKYVYYRHYEELEDHFTKDHHPCPHPACLERKFVVFSTEYELKAHFASEHGDEVKMSAAQRRQALTIPLQLQYRSRDDEDTELGPSARLERAAIIIGGGHNVSSRAARSGRAAVSGMHHSRSEPQISQVASVTPGQEGASEVSSLTFTAEDFPAPSSSQQSAIGAMGRWAAFTGTQASTGSLSEQDFPALPTLSKNQRRRIKDQQRTLAERLSAAAQPPRVLNRALPGSGGGNGAAGAVQSGAVGSFGQALPGTADFPALRSSQSVSSTASAPSSQPVLQQAQLPSSSSLVPRAVDVACPTPVIRSPPGFGQDEFPALGGGTTARQAPLSVPSAYSDAAMGSTAAAVGPSLTQAAVLNNLFLRPGSAAVTTSMTTPAPGVAPDAPVQLKAEDFPSLPGTSSKAVGKARVFKNSPKASSVKAEISCHNPVPDKLSGPAAAKSGGGGVSDRLKAANKALIERIKRQLTGAAFDTFRQQSMLFMREELKAEEYHDYMVKLGLLSLVSELVSLCPDPTKRRLLLDVHRDFICSPAAQDPSLAGAGWMPPEAAVAKANRVAQHSAWSCHRCSLCNAPDDSRCEACGSSRPKDEEQLEAATSTVAVEPAAPGHATRGSVAGPQRPLQRSPDADDFPPLLSKAARHGSTAAGDRREIVMEEGGPEVVAASPSANATSKGKKGKGSTIKIGLHTSGASGSSGGQTHPQNVWTQPAFKAKVANSWSGQGAGKLAKMHGAISDAWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMU4</accession>
    <name>A0ABQ5RMU4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="847" mass="88146" checksum="357AB5EEDCA7FAF5" modified="2025-10-08" version="1">MVTFRVHPVAAVRRRSSSISSAFAWAAGLLIAAVFATQSLAQLSSVLAPVAVYNDPRPGDGTDLNRKNNLVSSVLNIAAVSGVSGSVSVVTGTPTSLRMSAKTHVMAATTLIDLDENSLILLQGLVNQPGYQLVITAARDIGADYSAQLTRFLGASPECGQAPLSRSAILIFTADGLPSSLYPPMTTFTCQLGTPWYRASDGSSPVYMFKTPGGGWVKLLGADWSVPAPRRPESWQYLLLFVPPSPPPPPSPPSPPPRRPSLPPPPSPPPPPPPRPPPPPKSPPPSPPPPPPPPSPPPPSAPRSPPPPPPPPPTPKPPGFPTPPPPLPPQPKPPSPPPPPPPASSPSPPPRPPPSPPPPSSPPPPSPSLPVSRPPNPPLPPNPPPRPSPPPPQPPLDARLIILTDLVEPDDVLRKTQLQITALQLYPGDGNVMLSSTPGFAPVHTVFDNTFLGLSGPNRTTLRRLMLQGLAVTVIVTPETSQVELSSLLSSLLNLTSAICTKRNMNGKGGITAVSVATYSGILGTPTVLGSMQATDNTVAMVCSGSGRRVMVVDGTGEAVVWEWAVGPGILRLVGYDFTSGGFSTAMLRNPASLGPQDRLRIAASVAVAYPRSMLPPPNTPNGPPTPLAPNAPVYSPQAPGNKKQPPTVKAPLVVVLIDDVFLPDSNNKETLIDGISAITTKPGQVQSPSNTALVHIAFDTTLTRLNATSRSQLKSLLLASKTLLSVIVTSGVSDADLSKVLAEVSGKPGLTCTSTPVQDDSRVDLVAGASNDLRSSGWKALSNTRSSVCNAGKVIFALRSNRAASVVLDIPVGLTAVRLIGYDFASGGRGDNRKPLTTCAIVSHPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMY8</accession>
    <name>A0ABQ5RMY8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="727"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1107"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1409"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="429"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="489"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="528"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="623"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="685"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="768"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="788"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="833"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="886"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="941"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1040"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1056"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1077"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1161"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1174"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1211"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1226"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1265"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1315"/>
            <end position="1326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1478" mass="149040" checksum="753F88A562710C8A" modified="2025-10-08" version="1">MEDDATADHLEPHGENESDAPLERASSGPFHTGAGKRRGDITLAELQEQFYLLQHQFELFQEGRTRPPTRARDEGSAPAPVPRERSRDIGGGPGPVAVASGPRSQSVPPGARMLRSPSPADSRHEQMRPRQPFAQQPPRASRPPAAVTTPSPPGAAPPRPRPAAAVLDRRLPSPPPLQNPRSVAKALPEVPAASGAAGINKTPEKKKVKKKVERPPWRDDFAGESSPWSRELAQEQAEAVAAVDSLATATGGTDNGSAAPVQATLNPGRGHSSRESEPWSRTRASSDGGDRDAPPSPKPGPVVALGEIQEGLPKGVLAPVRRRPQPTTAASVRAAAVAAAIEVGRQQQLLEQQLMEQQQGIRQQAARGGMLLVVSSGPGGTAVAHQGSPVELDAARTSPGRGSHIPSLPSPGSESRPRTQQSRIPTLPVQPEQQQQGHQNPVNGPRQLEPATTLALGDPLGDLGGGGVHRLVVDASVSSSFSNDDPLFSSTESSQTPASSTVSSAQQQRRRRQWAVPGAAVAGGGNIGHAADGVAGGARRASSSDGAADGAVAPAVLCGPPAGVTSPSAPAPGRAFNPTPPSSRPPSRPASRPASRPTSTTGDATAGASGHHHPNSSTAYAEEDARQHSSPERPTGHRISSTGGYHEARGGGILPASDLATPDLTFSAGISAGGCSEDSFSRRQTPPPGPPPGPSPTADAGSGGSFHLAAGAPVAFDRTAEIVLPAIANEADSGPHKAGMGRGPGPGPVGPRNKEEYFRKLSALKRSLPHDSMRRVTEDGGNHAGVSCSGGGWGGGGDSSSTGNGGGGSAGPSRKWMDPGSGDKPLSATPVDAVGSAPAAVRAARSRNLTADALQSNTVRGGDSSGGNDVLDAGEGTAGDPSGPARKPRPARGRSFQHQRSGSFGDAVAAGPSLGGAAEEPRISDSGQSARTAPQVPVQLPGVGSSPPHSPSRLSPHRVPRGIQPGAGPRAQPPPPQQQQQHVGGTAADASLGGAAPISAEQYMAFAVQYPEIAMAAAQAVMNTNGSRTRARWNVPPPQALEAAAAPQQQQPPQPGLAAAGATATPGGGPHLWPHQQHPPPLPPPPPPQQLAAAMVSANSLGIPAVPLLCSPSQAPQPRDPAQRPFPSGGDNSHDLRRANSEGPRMAMGRGAGVGMQDTVPTLAAPPTSAPSGSGPGGFGPPAGGPSGLFYGGAHPPQPPQQPPMVQGLFPSGSAGAAACWPGPGPGEGLPDGNGRLVGGAYTSAGQPARPAIAQTNEVWTGGPGPGPGPPSQVHPSGFPPPPPPQWNGYGYGPGVRPSGYAGLPGPGPGPMEPHGPLGHTGPLGDPYRPPIFKRVSNGHHDGFGAAGLGYASAPPIASGPGYGSGGGAAGGSDLPDYLRVSMSRMVAGWSAPNAKHGALSGFNDVWQHRNNSRPPAGPGVAAFSSGGSPAVPGGDVAVQARAASGGGGRETGFEGLSLAEVLASKPKKETPNFKKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNT8</accession>
    <name>A0ABQ5RNT8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP diphosphokinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21262:SF31">
        <property type="entry name" value="GTP PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21262">
        <property type="entry name" value="GUANOSINE-3',5'-BIS DIPHOSPHATE 3'-PYROPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13328">
        <property type="entry name" value="HD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="2">
        <location>
            <begin position="208"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="RelA/SpoT" evidence="3">
        <location>
            <begin position="404"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="735" mass="80132" checksum="9DC7D0FB0ADE490F" modified="2025-10-08" version="1">MAAAYSVPAGQGYLATSPMTLTRSTPIPFGTKRLPCGGRSTAPAPTGSTRWSGAGAHDGFVDGVRAEDYYQRYSLSASGRRRSVASSPKTTDAKPVASSNAPVTIGFSVGTNSPFADDGKLSIPRPASGTASQRGLLSRALSSEPMKEHELATPTLPAVSADPSMFFPGVPLDLLRQAQATFPVFHHELVVTAYGLAAKAHANQLRKNGDSLLSHCVEVAKLLAGLGLDAETVAAGLLHECLSSDVYRSQLEEFMPPSVVSVLDRVNTISEMSRLYRQHSNSGSFTEETFRRMLVAMEDVKAVLVKLADRIHNMRTVAVLPIERQNALARETLEVYSVVANRLGVWCLKAELEDLAFSLLHPEEYTWLKNAAESRQGPVALEATINAIKAALHDHGVKYEDISGRPKNLYGIYSKLKKDGKPLTSESLDTVYDLMALRVVVASKHDCYAALRAVQSVYRTMSTRSKDFIKDIKKPNGYQSLHETVYGDGDVPVEVQIRTHKMHYIAEYGFAAHWKYKEKLDSEDEWLEKETQYKRWLTQYKLGVHDKKVRPQGSPPTDGSLTSLGVAYLDVPEEQQGDLLDPFLRHQRFKLQVPAKTEVSVLLATSDGVETKEFPLGTTAHHLWHELGLGSQPGYALTVNNRLPSGEAALQSGDLVQVLPLSAVLSRSPQDRAGSPGWSLAAVAEEWDEEISSGAAASHSGEGYPDNVLEVYSRDGVMTWAMGPQTGSSVPYANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNU0</accession>
    <name>A0ABQ5RNU0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">cGMP-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_000756</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0140">cGMP</keyword>
    <keyword evidence="6" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="912"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="1272"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="68"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="336"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="578"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="953"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1355"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1589"/>
            <end position="1678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="463"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="611"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="622"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1355"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1597"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1630"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="941"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI58969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1761" mass="178569" checksum="FAA3E7096D031148" modified="2025-10-08" version="1">MPGRLAAARASREASERSAHLLPRRTASTQGGLAADLEDASLDGESNAISCASGRRLLHNTCPEASVGQGFSVRNPQQQQQQQLHDLPTSSQSVTFLVSSGQHGQNGTPTAAAALSHHAGAGGGSEPGSPARPPRTPSTAIPPLPSAGTLVVQTSGGSDHTSASIAAANATADPARGQWQGISSSSFHNRSNIEWTAAGEPESPRAEPAIGSGPVAAAPAFAFAAAVNANTSIPSTASGAGITAGISGAAATATGGMPSTPVYHSSIQQPVGTASAQVWRAAVTPVERSRPSLVTAIPVRHSRLRIAATASELSGANCSGTLGDGAGATSVAAGMGSATMGGSSPHHSGSQQQSAMQMLEAGSAAGTPASEGIAPSIRDLPASLGNPPSEAMAAWTATATRAFSGGGSSSTCGTPAGTVGGTGSFVSRGRGARHRLTPAGSVGFGGVSELFADGGRGEEAPSGESSFTVRQTPSDMEDELLERARARRAIRSITLANVALELRSPDGSASVGGSTAVRRPGMAIAGCAAAEMSALTAGTYGHAVRAGTTGAVIGTATMPADASGAPNGASRVAIRLNHQPGHQRRPPNRHASMPAMKRDGANSAGVNTDGTDGGRCGGGGPRRSPLTRPPLSGGLLPEDAVPEDIEGEDMTSLQYSALAGGGGGPGGGGDLPSGYSRMPSDSPITSQMSLTSSVVCQGASSTTQQRQLSVTSLQQTITGGGIGAAAGAATISSSGVGTGSGSTGTNAIAASLALAPAQPPQPLSRLAAPGFPPAGVPPALLVRLPDLADGSSGQRPSFEEEITRPTPAVLVGSSAIPGVVTPAPNSSCVGYVGAAADRPSQRCSRMLSGTHAQTHSSSLAALHKAVEKLMLQDSGLYAKVQASMKGISSLAKETNKLEFVRDASGATVINQYVVVKTLGRGAFGKVKLCLNTLDGQLYAVKMINRAHLLRQLQKPARPLRRRAPPPRNTSDTGLMDMHHRAQASDGNLSISHTNLNSNFGNAAQVAAPARASMDDTSPLAAIMREIAVMKKVDHPHVVRLHEVIDPPGSSYLMMVMEYCEGGCVMETRAQTGLTPLGEDTAREYFRQACLGLDYLHYNNVIHGDLKPENMLVSGTGVLKIADFGSSRFMGGDPTDATKTSCTPAFQSPEEIGHMPVDPFAADLWALACCLFCFVFGRLPFVGSCVVDIYRAILTRPHVFPEEAPCSPALKDLLNRMLDKDPNKRLKLRELGQHEWVTAGDLLPPLQFLQSQGSAGSGGEAPPPVIHVTHAEQVNAIDRSSLVSLVRARLKEKACQPGEYLFKEGEEAHCVYMIMAGVVELTQHLRRPGGTACHGGSHGHGGATATHAVLPPGGGAAVAHSHGGGGGGPSTTDGGVASGGELPGGDHDASFSVDLDESLTLDHPDMGQITEAMNIVDGKLHIDRQQAIEIRRRMQGSLLGDNAEYVVEVKGPGSVIGEIGMDNAAAAKHRVSARARGPVTVVKLTEENFFKALLQMYGEGGGGGGSAASTTLTASSASEAVGKGGGGAGVANGGAGCAPGGGGGGTPGLSAQHLMVMQYLEDSGSYACFPPASASGAGATPGRFSLDYGAGPSTLGSRHNHPYSHHHNHHHHHYNYQNSGGSPHKLHRNHSAFGGSANAAAGEHDRSPSPQPWTHTAKPAAALQEPASSSLATSAGGTGAVNAASNSISPAQGSNAALPTRRSGAFHAAVVPARALGLVGAVAGGGSRVPEVHPMLGMLNTIYSVNASRGGAATSEEGIADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNZ7</accession>
    <name>A0ABQ5RNZ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001135</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF7">
        <property type="entry name" value="F-BOX AND WD-40 DOMAIN PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="216"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="256"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="299"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="77"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="128"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="53445" checksum="F2C89F9D32074BCC" modified="2025-10-08" version="1">MSRIPPGGRGGYPPPAGKYSAARSHVMNDKYMEEFERAWEELHALHMGEGGGPDGVSAAAGRAGTSLAAHQQNIYAALEDPSGPPPSYYQAPTAVRRSAASPARGSRSSAGTGPPALRAAATPSQPLRVGGPPARRPSPAAGGDTASRFPARVSGNWSSGPLDPAGVMCDLSDRPNTCSAADWSRGEVVIGSTDHALYVLDVNRGLLKKRTLYSKTSGHSEWVTCVTYASQGRIVSGGMDSKLWVWPASGNKGMVAEAHFGPISQVKYDRSSDLVASASYDKTIRLWELGARPHEVGCLQGHEAPVLEMQISTDGRIVTGDRSGHVLLWDTSSGGASWRMRNVHQGHVTALAWFDEGGGAGEGEAAGCFVTGGQDGVVRVWDPRAKANVAKVQLHVNEQGHGAVGDIIAGGPAVAGMVVTAGADATCRILDPRLGFALAGTAKLINFPYSMTAAGCTAVVGCGDGSIHFIDVLTARTLYALGANRAAVRCMEASHDKLVCSGDDGKAIMYSFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPY9</accession>
    <name>A0ABQ5RPY9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="187" mass="20833" checksum="CF82973FC0F7D8BA" modified="2025-10-08" version="1">MSLTAPKAPPTFIAAQKKRALESNQAMMKALREHRFEDALEHARTALALEPNNIMVKDFIRLLEEKQALGETDDDSETEAAGPGHSRTASSDEDSEDEDEEGDTDEGEEDEESSEESEQSEEEDPAVLKALERLRLAEPRDPQEPRPQQITVAKRYAMRASLSASIKELLLAELAKEMASEDPLLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ26</accession>
    <name>A0ABQ5RQ26_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-vinyl reductase 4VR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026002">
        <property type="entry name" value="ATC_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14196">
        <property type="entry name" value="ATC_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046339581" description="4-vinyl reductase 4VR domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="42607" checksum="E449298C33ED9C98" modified="2025-10-08" version="1">MFASGATRLLSGIALSTCGLASISSSSTRAAFGCVALLRTCGSYLEPFKIIDFGSGPTATYASFSAQASGLPDAGSTAYAIPAAPNNDRSREGSGGAPDISPGATSAAAALGGSTTHLRATASVAPAAGLPSSLGPTSDVNAVSLPAAAAGEAPSAPASRRDKSASADVVYPSSRDDNLGVQRNWFNALFTQLNVIQRTGMAPHLKGRQKEVFEAVEREFEVLWVRHVRGKGLEGVAHRGMLMCCLAIATHKVLMYETGDDALVREVVRTNLGGTAVGLMMRLHKLRLWLLLKLLAEDPYKQAVRFLPSLHGDMGSLVVGEVAEGDSEATWTATSCKFHSVLAEEGATELLPEFCCQFSMQWLEVFSQYGVRVGLEESVGFGDECCKVRISRPAGTATPASRGSSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRD6</accession>
    <name>A0ABQ5RRD6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002078</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI60014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051419">
        <property type="entry name" value="Lys/N-term_MeTrsfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176:SF79">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="5">
        <location>
            <begin position="62"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="381"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="490" mass="53519" checksum="8E1D24E1D09481DD" modified="2025-10-08" version="1" fragment="single">MATLSGAAPDSIQARLASLRYAEKEYWNSRYKSQPCEFDWFYGYNALRRIVRTFVKRNKPVLQVGCGNSNFQEGMARDGYQVLNTDISEVVIDQMRIKHKDLPNLQYVVSDCRNMPEILDCQFGSVVDKGTVDALLCSKDAADNIHNMFREISRVLVPGGVFLLITLGGPAQRLSLVNRQEFDWSVQVCLVRRVAADKYAPSAPGRAIPLNDTTKPLSYIGPLEVKPDGTVVGLPEPFEPSSYFYTYVCRRSPLLLRGDNRTGKAGKVRLPDGWKATVRAVAKVVGGGLQLPQGILGRGRIVKMTSRTNFERLLREKELREVERFRIPAAEGGALPQLTWEQELEREQLRNPNTHPDHRQSGNCGNQQQDADLDLDLNLDPSSEQHQGAFNNHLTNNRKHPTATVVARPPKTAAAGLVTVMMTSAATATQDRATVLSGRLFPSFGFGGDGAVAAEDGGDGGRAIWSSVASEGDCGGCSCCGSYGAPSGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRT5</accession>
    <name>A0ABQ5RRT5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase I subunit rpa49</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002414</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009668">
        <property type="entry name" value="RNA_pol-assoc_fac_A49-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14440">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06870">
        <property type="entry name" value="RNA_pol_I_A49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="52053" checksum="E55F330D27E422B3" modified="2025-10-08" version="1">MSSKRQRVTVEVPSAGEAALPAVCYLPSVLEPSDAAESNDGPLKHMKFKVHSKHRLGSSIQMVHASFGDIDYIGRSDGSENRGLQTCYYALGVYQPGENKLQLVKVAGDRIFRLEPRLSDLIYEPTGAGLDFSTLEGGRKAAAHKLVSEFGSTRRRRRMDADEASKVTADKISGGAALQGMLTSMATHTKETGISKEEVTRRAFSQRTIPPHDPSATTAADAYPLDLLLSRNPGEADSDDRPSLVRELNAAEMHRLAEDEQMLQQAREKKKIHPYVLKKLGNLHRLKTSDSVQDQAKARERAKQLALLTVLLRLLECPFLQPKRDPVDAMAHELHVFKGLAEFFIGRFYNRMEDPVRGVRYERSIAQKQLLISYILAVAMVAEDGFLDAEQFEELRDILKMEPAVLLTCLRELGCDTRAAKTAIEGKGQIRIVHGYQCSLLRQTGRTIKTLEQCFPAINDKGKGRSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS91</accession>
    <name>A0ABQ5RS91_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_002609</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF12">
        <property type="entry name" value="KINESIN FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1188"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1366"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="258"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="910"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="432"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1194"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1410" mass="151162" checksum="BC268CFEBB794F01" modified="2025-10-08" version="1" fragment="single">MASPATLFSRQPLQDVAGETDQRDLLSAASAFFKWRQAALKANFRRELESILEEVAQEEEVTGQLQQQLRRTQKRLEETSQALSKATSDYRQAQGHIDQLRVQNDELFHALEAAEGASRAHADQARAASVMHQEETKRRQEEARQSKLETDALREQYRAAVAQQQALLEALAQRDREVAEASQEREHHKRNADRLQEEVERIEAIARATGERAAAAEAQAAARSETISKLYGELSGLRERYHVELSELKAARIGAADAQRMQERLDSVQATNDSLVAARDELRASLEGVMAKLTAAEQRLKRQAVAATMRDSIIDTIADVSRELARDGDESLIMQATTRLSSELDLALTNNTDLAERLTSADAVIAQQQGHIRRIAEECASLESQLREAREHRDAQHRLNSELAAELKRLAAESALGPIMALDGNGNLVPQEAPPPTPPAATAPATSQLEAQSTAVRVILADKGFGLGALSQALAAHPGSIQSVRAQLSECRALTEALHAAVGEVGALMAVRPFESEELSRKLPVLQDRLAKTETCLVAVREDLTLKQVHAVSLEARVLAAESALRGGASLLQGWSGLLAEVVVAEDGRLEPDVRLGLREELKNREEALHRALLDLAATAPPLLGGDPGYAAAQVVMREASNAMMDLALTLGDRLVDAHATRTRVLATEHLLVDRAAQLARFREVLAAAPGEIADMVERLTTVIPDVQAMGPAVEEMLRCVRHYLAAGKEPMVDVVALQEELLTLRGLVRTKDMHIQALEAVGTGIRTGSPTRRAQKMVPLEALHAAEKARDMLAGEKTALQNRLVDQRVLFVGARHRLVNFMASLRHSCNLAVVFYQWRATAAAIRANNFHADMVSAQEEQARVERECREKTAQIEAEMQLVQASHAAQVASLNAAHVAEVEALTAVHDAQVQAMRAAHQEELRQLKDKAVQAAEARVELLRRVFKDQAARHKECLATAVLYLWRFRVRRQVEARQRWQGFSVQLRFHAEPNNPTYRDIFRGPRMTIARRLALLHRDPSDLEAPHALTGRQIARMVLQVWRSYAYIKRQTQQVDALTEAVPTFMENLGDRRRLERAWLGLRLWAAGAATRRAHQAIDDYEAATAEARAARVVAEAARATADMATADMEEERGRQQRQLALLGVDAATATHAFASTQAGNGFNGIGVGGGPGGAIGVGVGVGGGLTSVATGTGPDTGPGSSGGGGGGGGGGGGGGGGGGDGGGGGGGYLLVPGQKADNPFMLYQLPTVEVGTQYMPNTNVVTSTATQASGHAAAAEVVAVARAAGLEVSTQPMTTSQHQSGGVVSGGQPGHLASWGVLQGQGDMPPVAGPNQTAAAAAAARRRAGVDAQPPLVSNFNAAASAALAAGARDTRSVSSSRMPAPTRWGRGVRSTGVGDGMGADRNAAPGRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSJ4</accession>
    <name>A0ABQ5RSJ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="558"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1413"/>
            <end position="1440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1549" mass="172718" checksum="AFFC882AA3473C2C" modified="2025-10-08" version="1">MSRGNKRSQRQGAISGHPLRIATHNMRGLLASHGSRLQAAVRQWAEQRLDVVCLQETHHTTLQHQCLFERDLRYATKQIHTQNWKIAAYSWSNSTHTAGVLILIRQGLASALAPKLATPQHTCTRGRFTDCILHWGGHRLHICNIYLPPGTTSMAAETRRQLFENALAATTTQAMEDNRDTTLIWAGDFNFVENPEWDSSAGRDGRQADRPITEMRQRAGPQMVDTHRYLHPRRRDFTHLYHAPTPGGSRLDRIYINEAKLPYLCHTAISFHTASDHRLATLHLLPCKNEAKGPGIKRIRMAFTDHEDLATSMAEWIRNQCQEAPSVEENPGNLIAWWPGFKRRLTFLAHQLNKVAQQRRQQPGAHTQEAYLGVQQAQQALDTMPHSPHLLQQLIDAQARLARVHIEDNARVAKKTRVHWLHAGERPGPALTTFLQTQIQDQQTAPVALQAHSGRLVTNPAQLPQVVADFWAGVCRAPDFTTPEDRQSVLTQLAAANIRLPEEADEVAGSPTIAVTELGFALKKSQPGKAPGWDGIPTDLYRKFWPEIGHTMAALFTAIGKTGQVPTGFLDGIIKVLYKKGDPKQPGNYRPITLLCSDYRLLAKVLANRLGPALGRIISPEQSAFLPKRLIGASVLFLRHLPHLLRKLNRQGLLAFLDFAKAYDTVDRNFLMGAMEAMGAGPQLCSWTRILLSNTRARAMVNGFKSRLVKMEAGVRQGCPLAPLLYLFVAQALFSWLKSQEIGLRLLPSDEDRVTACQFADDAEVALEGEESVPAFLEAMDTFARASGQRLNLDKMEILPIGAVDDHHQGSTINGLKVVTTATALNLPMDNQLQPPQPDWPEMLALAYTRMQRLAHYPLSAFGRAAAVSNYCLQTVTWHMEHGGPPPDTTLTYIERLAARLVDRKQGPEDQGQRFTGVPSRLLVGHPSTGGFGLLPLRQHIRARQAMWAVRFIRGALMARRERERVMLLDTAMNTVAEEEEEPVTSGNVRSLHPWIRTLATYLEITHPAFKPWALTTAQATGPWLGGETLPEEISRIVGALGHLPPLEDVSNPPLAPGDWCWAAALWGNPLLPNNSDNGGSINGNNHRPGLETRHPLMASCRKLLTVGDLVRVYDMLEHDQIPLPQYMTWLDWTQQFLDPSASPTQLRDKESTRMALSALCNDISPAWIAAARKVVNRLQPQQPDRVEDRMQQQMPAPPDRTTVMAMIVRRLGWRLPGDSGIVPLYNLTVSQATQLQGVSTAEERLELLSAFVQEAQGMPTDGPAPPCPVTNLQRFQATLDRLWTAVRWENKNKETLWRLVVDGIPIPGNTHLSRVPIKPCGCGSYGADTDQPCSPRLHHFWECPIAKAVREQIDRHLPLPTSATIEGGLTRQQLWLVQAPPGCEQAPWDVIALAALSSMEYGRHCLRAATREHRPQWPSTHRRRQQQQTHTAEQGPRQRAITDFFNRSGGFPQEEEEDANTHPIISTTQPAMDTPTQTATSPLDRAQARTVADFWARLHSFAALGIPHYGWDQVGPQHPILAIVGTQMRCADPVDITNHGAARTEQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSR1</accession>
    <name>A0ABQ5RSR1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein kinase ATR</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_002476</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00892">
        <property type="entry name" value="PIKKc_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056802">
        <property type="entry name" value="ATR-like_M-HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF69">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25030">
        <property type="entry name" value="M-HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="15">
        <location>
            <begin position="1095"/>
            <end position="1826"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="14">
        <location>
            <begin position="1960"/>
            <end position="2272"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="16">
        <location>
            <begin position="2277"/>
            <end position="2309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="389"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="651"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1040"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1351"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1651"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1733"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1912"/>
            <end position="1950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="652"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1058"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1655"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1923"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI60354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2309" mass="243658" checksum="6E909760C0D259E4" modified="2025-10-08" version="1" fragment="single">QALLTQCPDSSVHGSGPCLQKVLSSTLCCRSSALVRGGGGVLLRTILRPAVLLEIYGRNNAPLPPLPPPPQPQKQPSQQQQASEASSSFSSCSSVALAAAERELVRNLHHWLLEAQPPLPAEVRCSLLAAAVGLAGPGRVMCREMVPLLVLLVLGCLGGEERQLRAAACAALESLTASAGVGVCPRRLLLEGPLAPVVLHRVGCSVLQSPRLLPELCRLTKLNEAAVVRAALPHAIGPLCCGNKLPELRALARLVEMTPSELFTLHAHHAYAYAFWKEAQAFEAFIDAVDELMDEPHGHQRVLGNVSMARIVTKQTIIMAGEPSADWASSPSLEPPPSAISRIQTFMQVVAGQVADMQSDDPVQEFLVGELTASLFELFGKQLSLAPGAGGGGGSGAQGRGHASSAMAAAGGGSPLSRGVVQHQNQGGRGAPRSSTHWLMSADSSEAEALQALRSLSVLVVLLGHHLPGHVPPVMVLLTAAVSRAAALGAGTAAGGAGPCEELQLQSLAAWRVFLRCLSSTAPSLLERVAVQASVVLLPLLQSPRAEVSRTAAGVLTLLVVDNLPYVMNALSRMPPLPTDVPGLERVAAVLQEKLQLADGRKRLRQLTDSLQHESLAVRHVALRELRCFLAGQRTFVADLMARALSGRGATAAGGGGGSGGGGGGVNSSGGQSQKLTKRGSSGGAAAAATPATAATSAAAAGVPGVPSASSDAELLSSLLSALLRCCENAMRTTLGKDIRVRCGECLGLLGALDPARVVPERRAPPALLGADGVGPATSTSTSATVAATSRGLSVVGNDTEPYALEIAIVKQLVRIMETTGDLTVYRCTSYALQCLLQHYSGGEGSEGGGGGGGGYGGRATAASPEPEGLYGRIPPDIKPLVKPYLTTRYTLEKDVGRSARTKNDTLSGPAIFSANPRPSYRRWLNLWIRAMLRYAVGPHQAAFTAMTPVMRLDLPLMTLLLPQVVHAVLVSGHSDAIEAIGREVVAVLEAGAVGPSEADGQLELYLQALFGLLDVLGRWVKETWQVVSERAARVADLAQAPGQGQGPAQGGAAAAAGGSGAGDGGRGAGADGVGELPARAKSVKDFLESIPMETLARAASRCGAHARAVQTFEGHMRLEASKASQAKTGRLLSSGGLNPAAEITRPQYKALNADVSFLLEAYGQLGEPDGLAGLCAMRPGGLSEADQVLVAERAGQWNDAMALYESAVRREAVAADDAIAGGGKGLSALAPEALRSGRSNAAAAVGGELRRTSLDGGDGGGSRWVGGGVTGGSSSSLTVSQAGYIRCMLSMGRWQGVFSCVDGLLARRSDGPTGHKILAALGVSAAWRLGRWPLLRRYLQAAEAADGAEAAERNLSRGGGGDRPPGSGSKSGIAIPALHSGDQWELDMGYLLEALQRGDCPRARGLLEGYREESMAVLPALAQESYVRAYPHLVRLHLMQEMSDVLTLMDPDCGWSGISPSERARKLRWPERMAATQSSFTTQEPLLSLRRQLSALMGDREGEAAAWLATAKLCRANGHLDAATSAVLEAATRGVGGAAALESAKLLRARELHHRAAAELQTAIADLTTACCAVSGCSATAAATATASHSHRLLARLKLCLARWSAGEGGCAPAELRRMFEEALDADQAWDKPHFHYARYLDQLYQDAKRRQTSGPARPAGSSSGRGVATDRFGGRARVAIGDDKQYFEYLPDVIRHYGEAITKGHKHVMQALPRLLTLYCEHGSDQIARGSAPATVMGGGGGRGAAAPRTSREARAATEVHNTMKQLAGSALAPPKWLMALPQLISRIAHTHQEVGDVIRLALGRLAEAFPHQVLWSAAAVCRSNVPRRRDLANEVFKSVSHQARRTGNHGLIALLEHFSALADQLIKLCHWQPPQTRGRPITSVSAGKEFGHLVQLLPSEVMMPTQDQFHAPLPPAALGGSSGGGGGGSSGSSGAGRGGYQDHPGAGSGGLIKIRGIKDELTIMQSLMKPKKLTFIGSDGLEYSFLAKPKDDLRKDYRLMDFAGLLNALFGGHAASRRRDLRIRTFAVVALTEDCGILQWVEGLLPLKGAIEDIYTTERVFNRKEWYSWLRKFYETWSEPSNKAKQLSKVLEKLPPRLHRWFLTRFPEPATWLSARTNFTRTNAVWCMVGHMLGLGDRHGENILLDSTCGDTLHVDFGCLFDKGLTLEVPEMVPFRLTQNVVDGFGISGVEGVYRRCCETTLQVLRQHRDTLMTCAETFLHDPLVEWSSKSQGRGAAAQDGGSESENPAAKDALATIEGRLSGTLLGVSAVPTLPLSCEGQAARLIAEATDKENLGRMYVWWMPWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSR4</accession>
    <name>A0ABQ5RSR4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_002830</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SYF2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013260">
        <property type="entry name" value="mRNA_splic_SYF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF553">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08231">
        <property type="entry name" value="SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="12">
        <location>
            <begin position="7"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="244"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="353"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI60633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="531" mass="59122" checksum="632A8371C6BF298D" modified="2025-10-08" version="1">MENPRVWFDIQIGSEASGRVVMELFADIVPRTVENFRALCTGEKGVGKSGKRLHFKGCVFHRIIPDFMCQGGDFTSGDGTGGESIYGDRFEDERPGLALKHDKPGILSMANAGPNTNGSQFFVCTVPCPWLNGKHVVFGRVVEGMTTVKRMEVVGQRSGKPSRRVLITDCGQLPSKLQMMLKLKAEKEEAAMLRSDPNAVNPDQESLQRLKALKQQQQQPPNRASAPAPSALGTIAAALGFGKPAEQQDQQELQEGTQDRSREEADGHGAQQGDEGWDRGRGSGPGTEGPDDQPGSLSGDGGVEEADPYAGMSAKQRKLLELRQRLQQCRKQNQNAVVAEKKRQKSPGAETRGASDDKRRWYEEKQKRRAEDLERLGLDSSQAHRLETAEAAALKYEKREKKGGPTGWGVFASENLYQAYLKRSENVPYTQEDYEKAKAADPEFYRDADSLMYGQNPKLPAENVDKMVAELVDRDRKKEEFSRRRRYNDGADVDFINDRNAHFNKKIQRAFGQYTAEIKANLERGTALPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU62</accession>
    <name>A0ABQ5RU62_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trimethylguanosine synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cap-specific guanine-N(2) methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003225</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78479"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="Rhea" id="RHEA-COMP:19089"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="Rhea" id="RHEA-COMP:19090"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:78508"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78471"/>
            <dbReference type="Rhea" id="RHEA-COMP:19085"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:78472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78475"/>
            <dbReference type="Rhea" id="RHEA-COMP:19086"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78476"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="9">
        <location>
            <begin position="134"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="215"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="275"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="391"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="728"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="854"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="222"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="482"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="728"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="923"/>
            <end position="937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1247" mass="131179" checksum="5CA3A941DF2FD79D" modified="2025-10-08" version="1">MARQVLIDKWNGAQDVRLSTALDLDLSDTTLYRLGRLVITSVRLHDVGLVGGKEPVNAASEAPSDTEASSLSAELASLGLPTAFGKKGSSRLERRTPGGSLKSSRLAHKGELRQSGSRKGSLGGVHGIGGFAGSHPKTPWLRGFDEQWGCHYYYNTKTKESVWEAPPEGFVPWEEAAEVADGADEEAQRDVRESSMAADRPQQLTLDNCNGAGAARSSVLSGAGTDGTTGTERDAATGAAATPGGEAEARAPGPAPRMLTEEDLLREMGLWQDIGGSQAGEGLRSQAGVTAGSAPPPPMAQAMGASGGAAGEPSGAGAGAGELSQGAAAGAVAGGDCLGAGLLVNLLRSSASQLQQQQPSSAVMTQTPQRVVPPTALSEEQLLARLSLSKSLLMPMPPPPPVPAPPPPSPPPPPAVAHTDSVSGTAAPASSDAPEAPPRMLTEEDILRSMGLLPASPELGGGRTPVKEPRPASSLAQAFPPGTKPPPPPPGFSPVQGLAAVTAKYSSPKSNDFDGFQSPPPPQQQQQQQHGGDRPPPYPIHLQPNQERHPHAGHQAVQYNPLAPPGSGAAPRLHLPHGFHSVPHPQSLYGHDMSHHPHRRPHLPGPPGGFPHPHPLPFNHGNAAGVPHMGLGLSLPPPPPGFGHPPPFPPPLPPPVDMWSHPGGPFGPPLMPVLPPPPSSPPPLGFGLPQAGPAMPYGPLPYPYAGHPYPPYDMPPYGMPPSRPAPATLPPPFLMSGCPPGPVPQPSSAEITLDGGLVTPPQQHHHAPQPLPGSHLHNHDHYQPQRQQPEHLNRHNEQQQQQQPPLASQVCVDADTVASGQPGAEKGLDVNRRAVTWTLEELERQLEVMVGTVTTEEDGAPAVTAPAGQRQEVREGAEPGLKGPGPAEDRQRRQKGGNDKAQQLQQHVVRHPGTAVGRRRTGTGRSAGATTEASSADNNDDDTAFYDTFANGAITAAAAAIAGTATPLPQRVGTAANALVGEDDGTRRVALDPNTAAFVRKLLPRSAAKYWMQRYSLFSRFDSAVRLDTEGWWSVTPEVLAAHQAKRSKEMCARGLVAMDACCGCGGNLIQMAGLFAVVLGVEISPKRVEMARHNAGVYGVEHKCQLLCADFFKVAAGIKVDSLFMSPPWGGPKYQHVNTFDVFFPLVGFNKSLLRLLDVSLNCVREQDGVVVVFLPRNTDLQQLAAVVPEHAVWEVERAYVNNKLKGITLYCHPGRTSDEPNVRMEAEAEYLLQGPARVEVAVAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUP8</accession>
    <name>A0ABQ5RUP8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47215">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47215:SF3">
        <property type="entry name" value="FAD-BINDING FR-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="43054" checksum="141C24A4941DE63E" modified="2025-10-08" version="1">MIVHSIHRCLGWTGQRNQPFGNPSSLRRELNRNRSSRRAGRASTVAVRAGPVEIDEDDLIQGKDRHKWYEAKFCAEDQPEWNIGRFVASRQVSPEYRAVTVECEVSRERIPLRAAYKAAGQRASLRINNGVERSCSVASPPFPESINIGPLFKVRGDLFAHEIKTAREPISVKAELTVLVMRKEAPEVYGMGYDDVVEVGPFVGTGLDLRSSPLMAMYSCPTVVIFVSGRGIATCMSLLESTSDVPNLSLEFRRDVRVYYKAPNAASIAFKDRFEEWEKLGPLKVLTTTTSFQDAFDDDDSLTYDPETTGAVILTGGDAEAEKEALEVCKDAEIVKILTDSVEQAAPVFLSSNPFKDWMTGKAPRPPPRNSRGAPVAEPQPDSDSTPVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUQ0</accession>
    <name>A0ABQ5RUQ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF263">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="51"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="191" mass="21834" checksum="D1CC734E5E7E83A1" modified="2025-10-08" version="1" fragment="single">MPAVIDFCWKGGNTGFPVRAPPAIRRGDFVLSNTPVIMAYLGRQFGMMPGSLEDTTHVEQVLAVVTDAVAEGRLAFHPKDFYATHKIQVEESKPYIKQYGENRLPKYMRYWEALLDRNPDPQGFLIGSSIAVADLAVYHYMTAAEQHYKQYYDAIEAPRAKEHQMRIASRPRIQAYLNSNRCQPWDTDSMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVD4</accession>
    <name>A0ABQ5RVD4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04511">
        <property type="entry name" value="NUDIX_Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029401">
        <property type="entry name" value="Nudix_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43222">
        <property type="entry name" value="NUDIX HYDROLASE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43222:SF2">
        <property type="entry name" value="NUDIX HYDROLASE 23, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14803">
        <property type="entry name" value="Zn_ribbon_Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="129"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="305" mass="33589" checksum="407A6439D6ACB60B" modified="2025-10-08" version="1">MKLSFMALWNGTQPGWRNFTSNLDKVRLVCAAQRSHRHQAASCLCDHLCSTENFRRGEAGRLVTKVMSSATAVSAQGSPERMAEPPPWMQKIKANFCRVCGGRLAVSKPLGDKEWRHVCSSCGYVDYHNPKMVVGCVVEHQGKILLCRRAIEPCRGLWTLPAGFMELNESTAAGAVRETWEEANAVVHVVAPYAHWDIPLIGQAYILFRAKLAPPYTFSSGPESLEVALFAPEDIPFREIAFSSVAVTLRLYCEEVRSGTFRLHHGVIEKRPGSLPNDPGAFHLRDHVALDVTSATEMSGQAGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVG5</accession>
    <name>A0ABQ5RVG5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protease Do-like PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004041</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041517">
        <property type="entry name" value="DEGP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046449">
        <property type="entry name" value="DEGP_PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980:SF9">
        <property type="entry name" value="PROTEASE DO-LIKE 10, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17815">
        <property type="entry name" value="PDZ_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Protease Do-like PDZ" evidence="6">
        <location>
            <begin position="669"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="233"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="251"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="815" mass="86940" checksum="A5EAE529BB6D9215" modified="2025-10-08" version="1">MARSIIVGNVSSICCKAIGTSEQPAYAVLSNSHLSAASFSILAYAQGFTVKRRFTCVMPTQRLFGLGHSPARLVSEATHRRARLWANPSATPPNTSSWGVCTGTYFYEQRHLRPEGAAPLSRTFAAASRSVSCSSAPVQLPSQNAAITTTGGFCAPQPVTGAGDYMIARSGSVRSVPMGSEPSDDNCLVRPLPLATPASGFWPAPSTDNAGLGGGLRSTLCIVTCAKLGTSFGSGVGSRSSGSSNTFPDEPSSSSATASPSSSSSSYVVSQQLRPPPPPPPGSVRASPTATPKAATATATGATTASAAATAVAPVAAAFTPTRSYSQHIKSDRALLATLKDAPQQYARGMGSVLDSIIKIYTVHSRPNYTLPWQNQPKRESTGTGFMVHDRLILTNAHVVADATYVLVKRHGSGTKYRADVQAVGHDCDLALLSVEDESFWSSPTAMLPLELGSVPELQQGVVVVGYPTGGDNTSITSGVVSRVEVAQYAHAASHLMACQIDAAINPGNSGGPALQGDQVVGVAFQNLPGAENIGYIIPTPVVCHFLAEVRKYGSYQGYCSLGVLCQNLENPHLRAALGMSEGMTGVLVNTIQKTSNAAKVLKPGDVLLEFDGVRIANDGTVHLRQRERIYFSYLITLKPTGATAKVKVLREGQVLSYDLVVTPNDLLVPVHCYDRLPSYFMYAGLVFVPLTQPYLHEYGEDWMNTAPRRLYDKAMHGMMQKPHQQTVILSQVLVDDVNTGYQQFQSLQVLRVNGVEVLNLSHLKELVAGASEGFVRFELEDERIMVVDRSLAQDANRRIMERYRVPSSVSADIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWT0</accession>
    <name>A0ABQ5RWT0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL23c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004062</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_uL23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005633">
        <property type="entry name" value="Ribosomal_uL23_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03939">
        <property type="entry name" value="Ribosomal_L23eN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL23 N-terminal" evidence="7">
        <location>
            <begin position="18"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="156" mass="17537" checksum="860E0B1FA1C4C650" modified="2025-10-08" version="1" fragment="single">NLRGARVIEMAPKGKGDAKAVAAKAAKAVKKGSFKKSRKPRYSVVFHRPKTLKRTREGKYPRISTPSLQKLDQYAVLKYPLTTESAMKKIEDNNTLVFIVDLKANKKQIKDAVQRMYDIQTKKVNTLIRPDGQKKAYVCLTADYDALDVANKIGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXD9</accession>
    <name>A0ABQ5RXD9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleolar GTP-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004840</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
            <location evidence="1 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. NOG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024926">
        <property type="entry name" value="NOG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038919">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="OBG-type G" evidence="8">
        <location>
            <begin position="169"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="565"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="600"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="666"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="684" mass="76705" checksum="8FF4A0F4CA6EE851" modified="2025-10-08" version="1">MPTYNLKTIGVVPGAKDFIDIVLSKTQRGTPTVVHNGWAIQRIRQFYMRKVKFTQQNWNEKLSQILEEFPKVEDIHPFYADLLNVLYDKDHYKLALGQLNTARNLIDRVAQDYIRLLKYGDSLYRCKELKRAALGRMCTLMKRQGPSLAYLEQVRQHMSRLPSIDPNTRTLLLCGYPNVGKSSMMNKLTRADVEVQPYAFTTKSLYVGHTDYKYLRWQVIDTPGILDRPLEERNTIEMQSITALAHLRAAVLYLVDISEQCGYSIAQQAALFHSIKPLFANKPVLIVVNKIDARRMEDLADADRQLVDDMVTEARKISSGGAAAMAGDGADETILMAMSTLNEEGVMAVKQVACDRLLSFRVEMKVAGKRIGDVLNKIHVAQPRPRDGVAPGSRPPVIPPGVAEARARRAGGEIKKTERDLQEENGGAGVYSADLRKHFLLENPEWKYDIMPEIMDGHNILDFVDPDIDAKLAELEREEAELEAQFVAEGAMEDDDEGLTEEQQEDLRAIRARKRVLVGEHRSKKNSHRNQAVLPAKLANARGRTAEAMTRSLGALGIDASKVVDRVRSQSRGRKRERSASKAHGDEDGDVEMGEEGGKSKKRVHSSKSRSMSRGRSLSLAEPRPGSGLKDVAQRNKGIKMADRAQRRMNKMAKVGESDRAIPTKMPKHLFSGKKPGHGTRNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXI6</accession>
    <name>A0ABQ5RXI6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SOUL heme-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004659</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="entry name" value="Regulatory factor, effector binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_heme-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220:SF1">
        <property type="entry name" value="HEME-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="HEME-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="Probable bacterial effector-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046456457" description="SOUL heme-binding protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23399" checksum="7BD070F3C415F34D" modified="2025-10-08" version="1">MAGTLCAILTLLALGKAPEQCLTRNAPWFCHDLDCPHYEVVTNIEDDVQVRTYDAGTWVSTNLTGMDYDEAIRTGFTRLYSYISGANQERQRIEMTAPVRVEMTPGPGPFCEDHFRVSFYVPNDLQDDPPLPLSKDVFIDPSPVASYYVLSYGGLTNEKEILDKAADLVQLLHDAGLSYDFTSFYHAGYDSPFRLFNRHNEVWIKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXL5</accession>
    <name>A0ABQ5RXL5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alanine--glyoxylate transaminase</fullName>
            <ecNumber evidence="3">2.6.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004594</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.44" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06451">
        <property type="entry name" value="AGAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF40">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="7">
        <location>
            <begin position="77"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="418" mass="45767" checksum="0F0A4F1900A1A0EA" modified="2025-10-08" version="1">MRNAQKLLAPVLELGCRGYVAPARWAARAAEEVAALDNSHRAPAGVIEVPSRLLMGPGPANAHPRILAAQTLPLLGHMHPPFLKIMDELSEGLRYTFQTNSKYTLMVSGTGHAGMEAAIANLVEPGDKVVVGNAGIWGERVAILSRRYQADVVEIKVPAGQTFSYEMLKRTLLEHKPAVLFLCQGESSTGTHQSLAGLGDLCRQTGTLLLVDTVCSLGGVPLFADQWGVDCIYSGSQKCLSGPPGASPFFMSERAMEKLQRRKTPPATYNLDMNLIGDYWGWFGKRSYHHTAPVSTFYAMREALALVAEEGLEAMWARHQELHHRLWDGLRDMGLKPFVEKNEDRLITVNTIKVPEGVDWAAVVKNAMDTYSLEIAGGLGPTVGKIWRVGIMGFNAKPQNVDLVLDAFRDGLKRQGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYP7</accession>
    <name>A0ABQ5RYP7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052886">
        <property type="entry name" value="LCS_TC/CRSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23261:SF77">
        <property type="entry name" value="GROUND-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23261">
        <property type="entry name" value="GROUNDHOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="670"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="716"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="746"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="888"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="839"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="674"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="716"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="746"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="909"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="950"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1068" mass="106537" checksum="5B35E1063FE334ED" modified="2025-10-08" version="1" fragment="single">GGGGGGGLMVGPGSPLAARRNLASPVGHNGGGGGGGGCTSDLGFHDPVWGPSQNLGSGHLQLEQQQQQHSLQRLPCGLAVGGENWSKQCTLGGGPHLPNAIPDGAVYGVPGAVDVSFGGLVGGATAASQGRGPLPAPAPVPAGGSSSVVSAGVSAILNDTSAGGLLMGLEAMVAGVSGQRSLGPEQLSEEELALLGIQGADRDLLLDPTTDNSLDPLLDLLLAGGDALMGAGAAVTGVSRQSSQHQHQHQQQYSMPHNPPNDALLATQQQQLYSQIDVHRFSSTLAHQQPHPPAPVQPLIAAAGFGPARRLSDAMHATDGRLSNIGGSGVASVSSLPRTSASGLSLTASGYGGGGGGITAGALGGPGCIGSGSHSAPLRQHRLSSVSGLSTGMGQRPSRFAGGVDLSLTAAQPLEFSHHRGIVGGGADGSLGNGGGGGNGGVLMGAVSCDDRYSAMCGASDAPNGASHSGPMPQLTSLYGAGDAAASAAAIAGGLGRRRDFPPGALSGPHQQPSSQLPYLHPHQHVLNAFEGRHSAPGQFCIPGWDETRGLAHSGGSGNASHCFDPTTLAGRMLLSSTGPMQTATAPDLTRLLGIGTPTTAAAANAVATSASMKRLAAVLGSEWPLNTRQEIGDGGAADCNGRDIARRMAAAHARSNSASGLLDRDGLAGGGAAPPAAAACSRPSTGSGYNGAASSLSLLNRRDPQLTGRDQLTLTTLNTPSHNNNSGSGAAGPAAVAAALGAQGLRMPPQSPLMPSLSDPPPGRGVAGHVSPPPDTIVSLQGLRLRGEDDRIAAGEQLPPGSNLLFLRSRAAAVATAATAAAGDGGGDGSIGGSGGGNEQLTSVLQELQSLKRQQQQQIQEQAHQIQRQQMQIARLGLLGRIAAYDDPYSLGDTPPTTAEVATGTANQQQQQQQQQQTSPSNFQGHGAVAAATAPRQQMQHPYDDDNSIGGGGGGGGDGGRSSGLLEAVDGQLSDIDTFMELNMDLLWRNNSGFGSFMNEMRASSLAGGSGGSFTLGGAGAGAGTGGGAGGCNDMEVMRQNLRKDLRQNSAEQWEEMLKQLAGSGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZA2</accession>
    <name>A0ABQ5RZA2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MADS-box transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MADS2</name>
        <name evidence="8" type="ORF">VaNZ11_005771</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="49"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="156"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="358"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="419"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="548"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="375"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="441"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="511"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="662" mass="66722" checksum="67A8CBFFE558965A" modified="2025-10-08" version="1" fragment="single">MDRRDDEPGQQPQDEMDDAEGSETEDESEGVKGKLAVKSKGKSRRKACEKPEGSDQKRQAFSKRKRGLILKSYQLYKLTDARVFVFIVNDKGSSWAYASPGFARTLSNQHLLQMREYAQLTGNQRMSTEIMPHPHTDAEDRRIDDRPSVVRANLRATEAAGIPGSGGSNPAAASGGGTAAPTASSGGRNPHSTTQGCNQQQPSDMPPPPPGAAGSMGAAAAAVAAAAAARRAAAVAAAAAAAVTQGVPSLGLGHGLPSGLVDAAHAAGDGPRLVGRPINNNNKVNYRVHLEDDGGYDDDGVMHDVGPGSAVAGAGGAVAGGGGGGGFLTLGPDSIITPSGHKLAAAVGIALPADAGMEGSRGDLDVRDMPPPGPRGGGGGGGGGGNAFGAGSLERAGYAAAALAGLQPPLGKQARLREVSGIDSGGPPQPPIPRSRRLEHAPPPPPPPPPPGSVPAIVPQQQQQRRGPGAVPNFGHDSPGGNGSGATDPGPHPQPGAARGQLVPQQHGQHTGMGGGGGGGGGVGVQLQSVHPGDEDMRHLSLGLSPPRRQQATTTAQQLQQQPVSSLVPPPPRSGQTSHGSGSFTTAYVLPGAFSYGQVVQGSDGQLYRVVRELDDGEAPLLDDPMNLDEGAARVLMSLPQAHGTQHMAATAMQGVGGEGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZP3</accession>
    <name>A0ABQ5RZP3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutathione-disulfide reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_005972</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004776">
        <property type="entry name" value="PRK06116.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF2">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="10">
        <location>
            <begin position="8"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="9">
        <location>
            <begin position="370"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="52566" checksum="B533D99AB8943B63" modified="2025-10-08" version="1">MTNSEEHFDLVTIGAGSGGVRASRFAASLYGVKVACVELPFGFIASDDIGGAGGTCVIRGCVPKKLLVYGAAFGEEFADARGFGWAVGGGDGGEAATHTHDWASLMKLKSKEIERLNTIYGNILRSAGVTFVEGRGAILDPQSVEVTAGDGSRRVLRAKNILIATGGVATKIPIEGAEHAIISDDALALESLPDGPIAVLGAGYIATEFAGIFRGTHRHAVHLLFRGDKVLRGFDEECRQQVQENLVRRGIVTHPHCKPTRIEKLGEGQLVLHYVNAEGAELQLPVAMVLMATGRKPRVEGIGLEAVGVELDAAGAIKVDEFSRTNVPGIWAVGDVTNRINLTPVALMEGMAFAKSAFGGELTKPDYRDVASAVFCQPPLATVGYTEEQAVKEFSGNIDVYVSRFKPMKYTISGRDEKTLMKLVVHAESDKVLGCHMVGPDAPEIMQGLAVALKCGARKAQFDSTVGIHPTAAEEFVTMRTRTRTLYGTGTSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZP8</accession>
    <name>A0ABQ5RZP8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_005809</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="7">
        <location>
            <begin position="356"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="8">
        <location>
            <begin position="629"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="702" mass="78580" checksum="3D0DD40EB19CCC1F" modified="2025-10-08" version="1" fragment="single">MSEQLAIYRRCRRQYPGTAACVLLPAFCRRKAEASLRGMRHVHRFEPGTVLFNGVDWEGTLAPWPRLSYSVDVWYEAPDGGAVGYGPALSALTLSAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVAEVNVLGLSPESSDCPQVTLADGGKHPIVGRITLSLSIGPLRVVTRPYMLQGLAETAPYNMGSSTLRQYRARIDMETATLHMCKGTLSIKVPFLSFEHVTPKEGETEAVPMVNFAVTAMAARIEPELVGRKEAVRLLRKGAHRLLLRPRVSCCVTTAQISADPEVEALLSQYEDVFKDIPGLPPMRPVDHTIPLMPGVQPTARPMYRLSPLELDEVKRQVTDLLAKGMIRPSTSPYSAPILFVGKKDGTLRMCIDYRGLNASTVKNRYPLPRVDDLLDKLKGSAYFSSIDLQQGYNQIRIAASDIPKTAFRTPFGHFEYTVLSFGLTNAPATFQAVMDRMFRPFIDRFVVCYLDDILVYSKTREEHLEYLRLVLDVMRREQLFAKRAKCFWAQPQVEYLGHIVSATGVKMDPRKVAVVRDWPAPQNLTELRKFLGLTNYFRKFIEKYSIIAAPLTNLTRKGVFVSLESWTSQCQRAFEELKRIIAEDVTLAFPDYSLPFRVEVVSDASIFGSGAVLLQEGRPIAFTSKKFSGAEIRYTTGEQELLAVLHALKEWRCYLEGRPFTLKTDHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZR8</accession>
    <name>A0ABQ5RZR8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Eukaryotic elongation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SelEF</name>
        <name evidence="2" type="ORF">VaNZ11_005736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04094">
        <property type="entry name" value="eSelB_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01889">
        <property type="entry name" value="SelB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03696">
        <property type="entry name" value="SelB_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049394">
        <property type="entry name" value="eEFSec_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049393">
        <property type="entry name" value="eEFSec_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF11">
        <property type="entry name" value="SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21131">
        <property type="entry name" value="eEFSec_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21208">
        <property type="entry name" value="euk_SelB_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="5"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="573" mass="62075" checksum="742420E7058EBDD2" modified="2025-10-08" version="1">MSKRILNVNVGILGHVDSGKTSLVAALSTRLSTAALDKHPQSKERGITLDLGFSAFTLPMPEHMAHLPYDELQFTLVDCPGHASLIRTIIGGIQIIDMMVLVIDITKGLQTQTAECLVVGEVATSRMVVALNKIDQLYPVDEREKAVRRAIKRLGQTFAMTKFAGVNMVPVSAKPDSKAESPPLGIEDLKAALVAMVPPVPRIVTSPFLFAIDHCFPMKGHGSVLTGTVLQGSISVNEVLELPALKETRSVKSIQMFKAPVQRAVQGDRVGICVTQLDAGLLERGLACAPGTVPTFKGAVAAVEKIRFYAGRVPSHAKFHVSVGHSTVTAEMTFFGTCDSEGLTREAALAAMIANLDAMALSGKPMPFDQCRDYLYQEELYGLEGRPSLVAAAGSDEAYEARNNVVDDQTGRHFGPQWALMKFDQPITAPKDALVIGAKFDADVHGDACRLAFYGRLVSPIDPTRPEELARLNIFKLKTRTGTLERLQPDGTTAIVRNLIKKESDVTIFLGLQVTSGRGEKGVIAGSFGKSGKLKIHFPGGLVMEGRNTHDNIIKLQCKRYLFDPQAKKVLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S001</accession>
    <name>A0ABQ5S001_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="996"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="996"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1300" mass="133719" checksum="DFD66890816DF3F1" modified="2025-10-08" version="1">MSHVANCGISLNYLLTFARVIPDGYTTADVVQDIVIPATKDRCCRYVSLPAVPQAAVGPAKYFVSHRWAADFTRELVALVAGHFADAGDAAIETGLTAASTKTSVPGGARNSQEAEKRFQATGAATQIGGSADGAAADTSFPRGTGSINLIVRSPTVSSNVAGSSHVIPASTSGYEQQSRAPPPQQQQRQQQQNLHQEQRGSCAQLFRGPSSAGGGFTASRCITATAHSPGSSCLNRSLHPVGAGSTVPGMAGTCRGFASSSSRSSGGQVQGPGTPSGPQFPKSLRVRSSGGSRGNGTGARTTAGVIDGIGSYLSFVGNGRPSTASSNVGPSITEDALRVADPTMISYNFCVSAAAAAATAAAAAVAPTTVVLPGAAIITPRDGSGGGGGDVTGSIAVAATAAAAEGSGSESFGGSLNPLLARPSFVQHPWPNQRFSSMCSRSGCSTSTGGGGGSFTRHGLAAVTQHPSQAMAATAAATGVLGGRHSCGDKVPLTLRGELSTTVTDSCGTAAVEGRSTVTGVLPYRQSPATTPSCSDQHASRRATSANALRRGGSCGAVAAAASSASQASEASDTFAGDTAAAAAGAVSSSSSDRSRSSSSGAGSSRSRGGINAGSGVVDAVLTNADIVDRLLSQFVGREALLRDTYVWLDIFAINQHPSEQQANDLAQLQEVVGASEATLLVMDRYGTVLTRIWCLYEIWQTVRQRGSEAVCVLAGPLDFEELVHVWGDLEVERAQATFSADRDRILADIRTATGSPELNRHIQQALVKSAARDLEAVRAASPRTRLHGERAYRAAVLLRTAGSGAAALAAAREAADVFGSCVGHAHPDTLRALRAVAGCHKDLGAARQAVGLLEQQVIPFLEEAYGFGHTESGALDELGMLYYTLGEYDAAYDMLLAALSRRLRQLQLCAANTVAATQLSAALATGPCASALLAAAAWGCGGGGVDGGIRDFPSLEPLSGEPLAALLAAVTALVSELKVAEAEAAERRAMAEPIPLPTSTPLPPGSSGVGVRKASGAAAGGGGGGNVILRDAIRSSVEVVGLSLHSVGLVQRERAQRCRSAESSPLLQEARELLEASVALLEASSSAARGLSAMDARANLAALAMDGGEYQSAEQLLRQNLTLTERVYGKYHLHTATAANNLAMCLKRQRSASPGKALEAVSLYRRCLDVCSSHHGECHPQTLVVATNLGVLLADQRQWSEAERLLTWAVSSLPPQLGERHPDVLAAYRRLAEVLELRNKLFESEAMFRLTLQLAESGAPEARDHGLVLECREGLQRVQELLEAAARGQAAQGCCVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S086</accession>
    <name>A0ABQ5S086_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MTM0637</name>
        <name evidence="2" type="ORF">VaNZ11_005632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15852">
        <property type="entry name" value="PLASTID TRANSCRIPTIONALLY ACTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15852:SF54">
        <property type="entry name" value="PROTEIN SSUH2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="238" mass="25502" checksum="CF0C0D0FEB20D8BC" modified="2025-10-08" version="1">MNPANVAINRQSITATNVVQLLSPAGASLTCRCGISSASLSSYFAAALSARRISGKAQNAGSSMSTTGHGKVAHWHKLQLSDATCVATSASRFASLSRVLLVGQPGSMFRTLANRKWPSSLGPISLLVFLFGTILALIAAVRGVLVRKVKACKCCKGFGVVRCRLCNGKGTVDWRAKFSYSETCPLCTARRFVICPDCGGHYHRRLFNHAKGFRGTLETYFPMAAPNSNVQTRSNPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0B3</accession>
    <name>A0ABQ5S0B3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="123" mass="13567" checksum="F2207DC42B537051" modified="2025-10-08" version="1">MSAPKPKADQGVLLFDEDDGEAATNDPQLQQKIHKYLAEKAAKEASLKQKPHPDGGEHTGLTDDDADDEGEPDYEVASRMTLKASSEAFLEVMAEARLEYEKEKEAARRKQSKAGSKEGTKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0B5</accession>
    <name>A0ABQ5S0B5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46503">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46503:SF1">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="326"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="692" mass="73885" checksum="765D1A3B16816EA4" modified="2025-10-08" version="1" fragment="single">MVPGSSPQVPVGLPGQAPPSPGQPATGYPMVPPGTQAVFSSAPKRAKVGNLTISCPMLVGVMGPGLGYVPMPITQLAVEAQCQISVGFVKTTLKSYFPQTWRASPLQLYVPKSTSTVVSEVVVENLNRDQMYVTAIVPADDVRKGGYKPSGAYGPAGQYEDGKEMNDPELFCLPLPGAVQPADQLRIVITTFEPLAFEEGSYVLRLPCEIPNSMIPEGYSHTQLLDVIVTINTGSSTKVNYNVPSGHRAIAGVQSLGTATLSVDKAADMPNSDVEVRYLVWGSDMFLALNVTPPRHPHPADPDPRGAFVLSVAPPAPEYTTPFPRSIIFMLDRSGSMSGEPMEYAKSALSFGLRSLTPLDHFTVVAFDHEQLWFTPGGQLLQATLENVAACESWVRGSITARGLTDIGSPLQTAMGVLSAFGSQQAMAAPLPFIFLVTDGCVADEKDICTAVEQRVGGLLHMKAQSEGALFLGQPGLGPPLGIPRICTFGIGPYCNHYFLKQLATYGRGTFDVAFRPHAIQAQMQHMLTAAQRPVLSDLTLTLPGVADCELYPFPLPDLFCGMPLLVAGKYSGNWSKEGITLNGRLPNGAAWSSNPVLPGKESDLPLDKIFIKNRLDLLTAKAWLAGNPGPMINQIVDLSIATGVPCAHTRTVGFETTRKDYDNMQKAASAGGRRKLDYTKYALGGAAGLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0C6</accession>
    <name>A0ABQ5S0C6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="6">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MAPKK1</name>
        <name evidence="14" type="ORF">VaNZ11_005605</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06623">
        <property type="entry name" value="PKc_MAPKK_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF9">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="65"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="NTF2" evidence="13">
        <location>
            <begin position="348"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI62869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="472" mass="52072" checksum="5775D8500ACAA7AA" modified="2025-10-08" version="1">MPPKLLLKLEVEPEPVQHNLDMASIRMSDDSLTVLTQSCNPVSFTAEGMLLKSDGLFYKISEKDIRILKKLGQGASSIVHKGFLIRENKFVAVKKINVFERETRHQMINDLKALCDAPNMPGLVSFYGAYHVPESGQISIVLEYVDGGSLADVLAKVGKIPENVLSKMTAKILRALAFLHKEKHLVHRDIKPANILMTISGEPKITDFGISAFIDSTLAQCHTFLGTVTYMSPERINNMSYSSPADIWSLGLALVELATGQYPYPAGGGPLQLMIHVLQEDAPLPPAGKFGEEFRDFVRLCLAKEPHRRPTAEVLLGHPFITKYASDPVSLKSFMQCVFNPHDKLDEIAIVFAHNYYALLNAGVGRLRDLAPLYSTESTLRYDGDLAVGCDAILAKLQALVHMHAGWKVTHEVVDVLCQPLGFDGSALVNVAGRLESPAAVNKPQPFTEVFVLSQMQAGEYYVANQCFRLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1M1</accession>
    <name>A0ABQ5S1M1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="833"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="877" mass="90105" checksum="B1586CD496FFA378" modified="2025-10-08" version="1">MFGIGPIRTRINYADAAPAGLPSRLSVGTGTESALQRTRSLLESATRQHKYVTSNYHLAADLRDDELAALSLLRSLLEELETCIMENENPPASVSKRKALIKVEKARRAVLAAVRECSSHVVESQGLFADIERFIELRPEEPVSPRNFPIKWSRKVPMLRNVPKRWEPTHGRSRRVEDSLVMSIIGLSALAEGSYRGDTPTSDASGPIAMQPPVLAAPKPSSPALPVVRTPLEPTTRHNNEASECSMMAVKDGGDGVAAALAAEAIGNSLSIGAGGGDTIQYDLISASETAAEGAAAAAVSVAKPQDVQNAALLLDQMQMQPPKPAVAADTEDGVRRPSLSYDVAHSGATTMCNIDSCVSVLPPAGSTLTAAAMGGGATESNMQSYAAYDNSADDDDVCDEDEVNSAERDAVSPNHQVSLLGCAKTLAAAATAASSMSKAAAAAAADAASAVTRSTTSTPPSRKEQGQTPGRGELTLSGAGAAVSIVPAGSVAAEAHSVRAGEPRAGSQESLHGNRPQLNSDLRRLPSGSHVVAPAAVMDLPLLDGSRVEAVLAGEAREVEVAAGELGDLGLTVANAAGAATMAAQRLLVPQQPRYVPIMPRQNLPSTTAAAAGSGGGGAAPVLPAAAPQQPYAYANPIGHVQHPAHAAALAAAATHPYGANGFSHPPSTYHHFSPHAAGGAAANLPGVYGDIATLNYPIHHSQPPSLPSSLRGYPVAGPATRPGGISGTAAVAALAAGPHHGRSQPMSLYQSLDSGQFPASAVAWDTVSVADTESGSQRNGRRRRGRSGGLMGLVRHVMGFVGVSVLSGAAIALGAAAVNTGLDEQQAAQQARQRQGSGGRRDQLQRISYGGGNDLIPQRTLQQLRSPDLLALGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S200</accession>
    <name>A0ABQ5S200_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039136">
        <property type="entry name" value="NUFIP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13309:SF0">
        <property type="entry name" value="FMR1-INTERACTING PROTEIN NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13309">
        <property type="entry name" value="NUCLEAR FRAGILE X MENTAL RETARDATION PROTEIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="179" mass="19130" checksum="5D7AED7D564DA942" modified="2025-10-08" version="1" fragment="single">GGSHPPPPRKRSLLEKLLHQELRQERSWLLQAIRFLVRNDFLQALQPTSTVSSTDKTAAECLDTPGVGVDGLQQQLQPPWQLWFPPGMPENPPDLKEALRLQLAAVAAEAAAADAEVEAEGQGFGDSGVAGNAAATNGAAAVAPVWAGGEFVEGEEGWEADVWEEEGEGDVDMHDEEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S213</accession>
    <name>A0ABQ5S213_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="125" mass="13317" checksum="FD7C83C0FDE37274" modified="2025-10-08" version="1" fragment="single">PGPWSDSALRDLLDRLAGLIPAESARRNSDRWPLGSTSPNQRFVTLPPTKGSWEAEIDALRSWLLARAAWLDKKLLKSGAGDGSGGGDPELATPPQPNLLTGMMGAVLGGRPVARAFQEGSRFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S222</accession>
    <name>A0ABQ5S222_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="114" mass="13168" checksum="A181242588B468F0" modified="2025-10-08" version="1">MRRRICQCCLHWHRRAFKRTAAVQEALSGEEAPGGGRVRAAAGPSNQVPRDLNDDEVFEAPELRVLMNDEDDEDSAFFLAFDHVMHRRVRPGFSWPIHCHIAIRRKETVYLTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S235</accession>
    <name>A0ABQ5S235_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="16995" checksum="60C2ADAAD90E3008" modified="2025-10-08" version="1">MLAKRKVQSEPATTLYAGRGGGSKFSGCYTCGGDHLGSSSAASGSGRSSGGPRMAAVGSGVWFDWCGKPGHVEERCFQKRQGKPKTIEGKPKVVVLTLVAREHKFMPHETEGARLLREYKQGIANRAVCTSPLLSRPHDLSNELKNNVPCDYVDSSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2M0</accession>
    <name>A0ABQ5S2M0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006687</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051858">
        <property type="entry name" value="WD_repeat_GAD-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017">
        <property type="entry name" value="GASTRULATION DEFECTIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="54"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="160" mass="16383" checksum="2F3024ACC591D513" modified="2025-10-08" version="1">MDEDDGSDNAAAVVASAGGHQDLGPGRSMQSESDRMDDLDLARAHLPVAFGRATGVSGVGNIGAASASGPRDKGAGKGTSQEPDNLDEEEEDGGSDEEAVDPRVGGVVETGDDDPYGLPITHEAILKGHTKAVSCLDVEHSGTRMAPLLASIQFWTRGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2R0</accession>
    <name>A0ABQ5S2R0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit delta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_007377</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233:SF14">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="163"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="190"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="222"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI64185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="707" mass="73558" checksum="620CC04F14F3261B" modified="2025-10-08" version="1">MFPRVTSWDNMTGFQQQPVKQVVGFHVEGASPSPTQPSLLQAAMTAGSPTPVIIPPSFQRETSHASRSAAAADVATRVLVPEVPVRVLDTSGSTTSQAIGAGVQQAAAEAAGAAIRATSSSSAPRPIQHAEKAEASHQGLGKVSSQASINSEASISSGLFSLEASTSRTPPPAAHAQQTSLPGPAGGAQSGPPGQQSGHGHSVTQGQGAALAAEGAPKGPKPQREMTKAERRALQESQRAAKAAQKAGVGGAAPEKPVKGAAATGDAGRAARGGAGVAEARPTGGKPSVTAAVGRSEGERRTDTAGGGAASGPIGVPVANAADREGAGTGAGAGAAPKKKKDPKAVSLNSTELFAHLQQYKRITVSRLLQLPAVGNVHPAILQLGLRYAEGSIRGANARCLAMLNALCQVIRDYTTPDGKELSRDLLRHLSDHVNFLVVCRPLSVGMGNAIRHLKLRISTISPDMPELEAKELLEQEIADYINVRIDSADKQLVNYAVAKVEAHGDVILTYAASHVVQRALVEAARAGKSFRVVVMDSRPELEGRATLARLLAAGIPCTYVHLNAASYIIREVTKVFLGAAAVLSNGTVLSRAGTAAVAMMAHAHSKPVMICCETHKFNERVQLDSITHNELGDPEALSVVPGKPEVTALEGWCDHPRLRLLNLKYDVMPAEYVTMVVTEFGMVPPTSVPVILREFRQSEQTVSGPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3C7</accession>
    <name>A0ABQ5S3C7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="530"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="657"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="806"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="917"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="995"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1393"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1495"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1626"/>
            <end position="1657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1772"/>
            <end position="1792"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="114"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="398"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="530"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="657"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="745"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1007"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1057"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1400"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1646"/>
            <end position="1657"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1792" mass="189748" checksum="09BED312274C701F" modified="2025-10-08" version="1" fragment="single">QPWMQPQPQPQPQPQPQPWTQPQPQRTSEPSAPSAPMPMPLQPRGSVEVATQSGMGEPRIDIHIHSTPTTTTTTTSPAAAAATSSTPAQLPIQYPPHAVPSAIAYVFPPAQQPQQQQRQQQQQQQQQQQQQEQQLQQQQQQQHQQKQLQHLPPQLRPVIMSAADPGALAGLSALSQLTSLSSLSHLSAAMGGPTAVDDPSLWPDLLETTPESLRQVSENTVARWRQQRAALAQPNLAVLRDMNPAMQLEPPQMLGPGPLGEAQYALRHIRNVGGGGDPHPIGTTDVPRADSPGKWLASRSVSGRLLAACPTTKEEVLGLAERELRQRERERLERRRRQLSPSGARGKYVDQIEEKSQEEKKEKKGLRERRFRSDRQGAGVHRPHEGNRSPDSDDSDDSSSSSSSSSGISGSNSTTTSRTSGSSSSTSSGSSGGARRDRKGRHGRDRPQPQDPTLPIISQSTINMAPVLQLAMKTHTAVSTSLAAVDAESTQRPDNGRDTADAAPYAIQSERGDDFGLDLAERLLEHMAEPERRLRKHGSGDSVKEAQQPGAQSTATAEAPVAANGGVGVKKGASKEEEGNEVVSPPTGRGHPSSTASELGITSQLDGYSPHARMVDVLHGPEVEQRAQKILDNHSVLRAKFLAAANNLRAEVSSAMGAGQPGAATAGPTHGTGLSSGYERYSDSEPPSGVRRSPALGPEPTLKQMADPAAVSSSLLSTSGLGFGEQPAEIGARGPGSAQAGGPGAVPSSSTAPAQQSLPTEQYQSINIYCTQLVLNPGRGPITVPGLPAYVPAPGPAAWAGVPVAIPSSTSDRPGPKYRDEDESFGPSSTSVLDPGQDRAVHMTTSGQPFQQPLPPAVKVSPVANATWLPPSGAPYKSLSGAADGEVTAAAVGPVGHPNDKLGGGTSGEEVFTVGLSRSPRRSTGGSDGLYKEASAASGSRPGPGKRLIRRVINSKDVSLDTSGALMVLSREGHSGGRHQDGPTASMSSVETILEKGEEVAEEEVESGGSSSDFVSQVPKTTMHGRTDGEVHPSSKSGSGSGSTHERQQSVLHASKPRTEGGAAEKDAEPILSLQRLEPPRKHYDQACGGDIGASKLLDAQRITSDGVASQQILPPQPQRQAALAMELTQLIMEAFQQSGGVISPAETGGVAATAAGGSGLSPQVAATSVPGGTRPVYLTDAFEASLNSTISVAPMTRAAAPPAAPPGPTQPGPSSQAAFLPSMLPNAAVPRGRGPTFLQVRDINLDTRKEGGVGASSNAVAAGTAEILNGLVDWEAEAMAALQGRPPMGQPHGAGGGGDRADRARQLLRAGGGRGLTAGEVLVMEPDAAQQRKLAALRHLRARQEAAAMERRARYQTGAQNFADPSRPLRPLFMSPSEYHGLRVGDDDVRVAGGNEELAPDRGRGPKAADSRRSLSPEERTRASLFPNQPTYRDGPDLQRQPHARSPLQQYMHGGPKRRRRWSDRRNEQAISPKAAAMLQHQCNFLNQVRSAMRPTRSTLTRSPCRDGGPPLGRTGRAVGAGVTFAAEAKPYRARETDAVRSVAEMVARRYEGGDWDTRGLESMYRMTPRAGGNSGRVELDQGDEDVDGQDHDNGWDEDAYPLGPETVCGGCNIPSCLVHGSRAARGKETPMGNPSAGADTIGSRARDKKENEDIRQRTQREMHRAALLDRWTAEMQALNAATSRVEATQKVAVASAQQAVAARRERRAAQLADMFRLLGEVGELADTMEQQTNDTKKMMRVLEEDAELRRAAQLNSLLMEARRLGGLVDESLLHTTSEGEGEASGPREAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3L4</accession>
    <name>A0ABQ5S3L4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007508</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="110" mass="11848" checksum="0323109E92675354" modified="2025-10-08" version="1" fragment="single">WMTPVGLCQNVLQAIPVHSLHAICILEETVRQTATKMVGSGLKWNTIRLQGLATVTDLLAFYLHEWLVQGGQVTTSPTGKVPLSDQGLKLLGVAVRARLALAAGKSYKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4I5</accession>
    <name>A0ABQ5S4I5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34958:SF1">
        <property type="entry name" value="ARMADILLO-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34958">
        <property type="entry name" value="CONDITIONAL LOSS-OF-GROWTH 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="541"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="868"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1364"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="868"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1566" mass="162839" checksum="B90C48066B52E8FB" modified="2025-10-08" version="1" fragment="single">ALARAITDSTSTSGAYPPAEVLRNLEVYLADPRSTDVVYEGLVHRACAEPLSLPLVEKVVGLLKAYLLKYVPSQDVMHGLIEFSSKVSELPRLSPGGRKAVAYVKSCLRQLGGERVSSSRGLRGSSNSLGNMSAGRSREDLPRLGTNIQSNDGRVTAMKVSVSSSALGRANDRYAAADALPSPTDADPGPGSGQMLAWLGHSDGRNTIGYQDIRLAQGSNRVEPPWAVPSRPLLLPPTPPGVTSAHARSWLQHLPDLAQRVTMAAPVPDMEMSSSSGAAGFPAAAPSVPLSLSGAPPISLATIHRCVLDLEEQDIGRHITEVLGEARAEAQQLQPATPRPRQQRGRGGLHGGGAGREGPGAKRSRPPRLLFHHPPYNRAEHLPLMEADVSVVVEAVCGVRAASAMAAVMGPLHEREATNASGQAEGMWQAAFEEDGEFGQICGGILVKLLVDMWTRAEPQDFFIIVLRMLCRALRSAQPSTRARAFDVLYNLYIHGAMLRTDAEEAALTAAAAETVTGAGGVQMPWQVCASAPVSPFVTGADVGGGGQTPHLAVPRGSGPLTPAPSTSEPQMPGAVPGGVKPGYHQVTSHSLGLQGSALLDSTGELPGMPSGPSGSRTGLKPGGDGAGMKQHMRKDSGIMRLPPPPPSPVASPSVQVTARGSAPSTTSGSKADITAGYDRWLRALLFRLLFELQQTLEDAEEVWRAAMGCLAQMCTHGGYWVAAWVQHMPPSALSCLLRAACNFSWSQKLYAQLLKLVPCVLTRPTDATTAGPASTGAAGSLPSPGSTKPLESAFAAPAALKSAVAAAADDSAAAATRGAAETEEMRRRLAEFGGMHELLFHFVRAPTPDSRTSLLTSLVQCCVETPGGSTTSSVSVSGSGDQQVHGFRSPRTSGSPGSRGLDGGGGPVGVLAVFQSLHLDSVCLALRAAVERPWQGMSSRLADVLASTSLDLGGAATNPVSPSGSVSASADYPLSAMHTPTPGLASPIAVRELLRPLLENLEACCVDSMTLPQVGEMRTHIDVSVAHIAGASNVDAASPPTVTAAAVTAAWRCFKDVCNIDKRQARVVAVQWLHKLLSAACRACQGQILLLSPLTLKTSPEASRLAAATSPSTGPNSPRALWNTALADALLHVVREAPQGCELLMKAITRTAADLKSATGPTSDLPGSMSPRLVAASPHGPAAAAAAGVTAEEVTLVACRRVMWLYYVGLQWMLQCGSAEARMPTVVDLADVVLAGLLQPDPAAEALQAASAVVGPSGSATAIATANKPPSGGAAYGAGRAATGGTSSGGGVPHSQLMATGMQQGQQQHPLAAAVQEPGGGMLISQQLIAGLLAFDPDALACTPPELLVTLLQQCSQNLHDMDVRSGGTGSHVGTPGERDSAETVAARAGGQAGFRSMHRGFLGRPCAMEGMGAPAARGTGDGANNSGRPKAMPLRANGSSVGAGGGGGGGEAALSGAAVGPWQDDWQDRRLALLLLLIARCSLDPEAFAKYSLSHVIKAQLSCDDLRCRYYAGVYLLKHWMLNQHDKYWRTLRHIIGTAQQLNDERLLDNPYLQMRTMLNVDHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4R9</accession>
    <name>A0ABQ5S4R9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SAGA-associated factor 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_008188</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SGF11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013246">
        <property type="entry name" value="SAGA_su_Sgf11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47674">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47674:SF3">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08209">
        <property type="entry name" value="Sgf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="69"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU261113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI64784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="179" mass="19037" checksum="F0B74B6695BF737F" modified="2025-10-08" version="1">MSTRRGAGPQNRPQITEEELLRRQIAIAQSFKGLLQNEASVAAQTVAAFSDLLDDCILDVAQEVHREARTGTLPSPYANRGRERERETAVSPKGSPSPGPSTRSAPIGSHPPPLPPKPGTKGPVDVFGQSHPPKATEVVSCKNCGRQVQAGSFAPHLEKCMGKGRAAARAASRRMQGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5Q7</accession>
    <name>A0ABQ5S5Q7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Target of rapamycin complex subunit LST8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_008303</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat LST8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="208"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="250"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="316" mass="34638" checksum="DE3DD39298A368EE" modified="2025-10-08" version="1" fragment="single">LFVMSSVVLATAGYDHTIRFWEATSGCCYRTLQYAESQVNKLEITADKTRIAAAGNPQIRVFDVNSNDPNPLLTYDGHAGNVTAIGFHKDGKWMYSGGDDGTVRVWDTRSQVCQRTYESRAPVNSVVLHPNQGELVSGDQTGHIRVWDLTASACSCELVPEIGTAVRSLTVALDGTMIVAANNNGTCYVWRMMRGASMTTHFEPLHKLKAHTNIILKCLISPDCQQLATTSADKTVKLWNLDGFVLDRTLMGHTRWVWDCVFSVDAAYLVTASSDSTARLWDLTSGEPIRTYSGHHKAVVCCALNDSAIEGRDADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6D2</accession>
    <name>A0ABQ5S6D2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alfin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008839</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045104">
        <property type="entry name" value="Alfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021998">
        <property type="entry name" value="Alfin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321">
        <property type="entry name" value="CPG BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321:SF98">
        <property type="entry name" value="PHD FINGER PROTEIN ALFIN-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12165">
        <property type="entry name" value="Alfin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alfin N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="235" mass="26801" checksum="52E54826AA043D2C" modified="2025-10-08" version="1">MDFPKTVEEIFEDYQRRRNGLLRALTDDLEDLYQQADPERENLCLYGTREGNWAVELPADEVPPELPEPCLGINFARDGMAKRDWVALVAVHSDSWLLAVAFFYAVKLDAAGRMRLFKLINTMPTLFESVSQRNKYSKSSAPQPQPGPVVKKKKFEDRPIESKYPSGRLLKAEDVSPALKGRQAELFWPDNQLWYLVEIISVNAKTKQAKIVYASGEEEDLDLAEIVREGHMALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6E4</accession>
    <name>A0ABQ5S6E4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008593</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF17">
        <property type="entry name" value="SELENOPROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="698"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="460"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="939"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1137" mass="115083" checksum="0FC13DEB10D5A5D1" modified="2025-10-08" version="1">MGESTGRARAALLGSFVADAATSSLHWIYNRNKLRETLTNPVDRTPEPEFFNPPSCPFYKSAVGELSPYGQELLPLVRFIATTGGCEPNAWTEYLAAHYNANNPPLVYMNHSAKAMRNAVIEEGKQWPDCGEALDSQANCFVKAPAVAAMFSGVPDGGLLIHAGINSAVRAQQNNALAVELAYAAAKVLERIIVSGEGVEAALRWVLTPEGGLEEAPKAREAFEEVLAKKDANPRSLMYVDGPDVKFGPWGAGCSNPGCLKAALLVALQAPDYETGVRQNLLLGGDNCSRAILVGALLAAKGGVSSIPAAWIAKTKEYQELSHVIEMLIKRRIASFGPAVEEGFAAATAFNTEAAVSTAADKVSEEKEAVEAATNNGVAKAVQEVDALQDATASAAAAVMADVAVTKQAVEVKTEASVNTAIYTMAAVEAAAASAVGVAEVKLATAQMGAEAASGKVLEVAAVKLAAEQYTAEAKATEAKKAAEAKLAEAQVASVAAVHTAEANLEATEAAAAEKIAAVTGITATDMTEMEEVAEAKVAEAKASAEEAIGVAQSYVAAAQAAVVEKVAVDQGAAAANAMDVKEAAAENINHAQAVAVAAVGAVQAKADGVQQTAQNAVGQASDVAFKVGARVHTSAMSAQEITASTQAKAQVAAGVALNGVQGKVAMAEQAAAEAAAEAQTKAQEAAAEAQTKAQEVAAEAQTKAQEAAAEAQTKAQEAAAEAQTKAQEAAAEAQTKAQEAAAEAQTKAQEVAAEAQTKAQEVAAEAQTKAQEVAAEAQVAATEAQTEVQEVAAEAQKKAQEATAEAQKKAQQVAAEAQKKAQVATAEAQVAAAEAQTKVQEVAAEAQKKAQEAAAGVQAKAQELAAEAAAKVQEAQTKAQDAEAQAKAKTQELAAEAQTKVQQATAGVQAKAQELAAEAAAKAQGAQTKAQEAEAGVKAKAQELAAEAAAKVQEAQTKAQEAAAGVQAKAQDAEMQAKAKAQELAAEAAAKVQEAQTKAQDVAAEAAAKAQEGGAQAKVKAQETQAKAHAVVAQATEGVASEGKEAATRASEIAADVQESAAAAVATATTKSEDVKLQVSGIAANAQKAAVGTVGNLMTAAAAATATAAATKAPQPGATPESGAKAKEPECCCIVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6I9</accession>
    <name>A0ABQ5S6I9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamine-rich protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="211"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="680"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="344"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="680"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="74962" checksum="FA6AFFA85AE45831" modified="2025-10-08" version="1">MPAPTNMGTNSGVEVRIGWDESEQKEFMKAIRQPLMVQTPLQLVIENLFQRLQAQANFIHQMKDKMERIAVKDVTADELLHRIKLLEAQQGDAAGPKLNPFNQQEILNRLEVLEKKLADAGLDKGSTNQRDRPTSRGDDADLDVRLTRLEKRLTGVYNLNGKLVAIEQIASGLGVAIPDLEYPTGGGPPPSNAAVHAGSIIAPSSVPPVQAWRSPIAPGGAATQPQHLHGHHSLPQPGPVEWARPRAPEGAAADADDAMAPAGAKILGVRSVSPGPNGVPLTDYMLSGASPHSAPQTRGSSPNLSPRLLGTPTSPRLIGSPSSAGAAHGGLGSAGGGAVSAQKVVQLAQEVTALRSDNEALKQTLDSLVHQVSGLRAGLGSNAVESLSRLQQAEEGVSALRAQVADLVAARRDAAARTGSEVTFADFSLLRSQVEGTIAEAKAQAAAAAGLAERELPVLTARVDELAEALKKKGEPRDIDALFHERINALEATMGEMKTELLGSIEAAMESAVKVDDLQALEEVLEAKAPAEALRLLQQQTHALGQAVAGIGDAISLRPDLGEAMRGGGGVGSAVGPLATKMRCLTCDQPIKNLPALGSLSAGGAAAVAAKGSFLPRLESMPAKEGLPGGPGLSIADLRTSKEERLAGLQVSSAGPVRGAGGGIKPADFDEYATGMGGLGTLNGSGSGQREGSPGRAVERSRAVRVPVGGGLGKPGGPPSAGKPVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6S8</accession>
    <name>A0ABQ5S6S8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36357">
        <property type="entry name" value="OS03G0148300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36357:SF1">
        <property type="entry name" value="OS03G0148300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046189231" evidence="2">
        <location>
            <begin position="34"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="234" mass="25881" checksum="DEC40B0A9BBA73B0" modified="2025-10-08" version="1" fragment="single">FLVSNAYGSTPMRRIIGPTALFLLLIAAAKSLAQDNYPDGSQEIASANDPVRDAVTIKLSPLEKRKKGELKALVERFKSLPGLQGTSIVAAAPDILVLVPKPGQSLDQVRRVLLQQPEVYELEVDENTYRREGNDKPYEELRKERLAKALTNSKSKIQDRIRTKLFELLQGKRKQGGGGGSGVKTGLDGQPQDKEAMKKAVTRAIKRATKRAARKGKRKGQRRVRKKDTQAAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S712</accession>
    <name>A0ABQ5S712_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008894</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045357561" evidence="2">
        <location>
            <begin position="18"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="342" mass="37045" checksum="D1F533B4B64CB98D" modified="2025-10-08" version="1">MILIIMVLVGVVNGALAMEALGGGLPASGSTYTSPLGGYSRPGTPVGPTLLNHQGVAMMQHVGPSVAEQQTALILQLQQQVQTLSTQLNQGGQPGMADFEFLSRYYQDPRAQQSPETGRFPFPRRIISGLPSGGINANNLVLLNYKAFPEWRDLEHAQRGQPDRAGSLLYEFGILMTALSIAELTAAGLESLIKSVAHMGLNQSWLAVVSNTHRTLHYNMNWLRELISARINCIIEFSSRGRDGLVALHHRYFATAERAVHDDVLREYDDEFARARQKVSLKYLAEKEHKTRTGGNTGVLLFNGTRQRRGRGGGFAGHGFPPNRASAAVGSSTSGASAPGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S731</accession>
    <name>A0ABQ5S731_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008928</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21324" checksum="57C19F32652EE55D" modified="2025-10-08" version="1">MSYNQYPQATGESEQANISVHDEGPEAMPNVDNVHIPQDDAPQSNYCYPAQDPPETPDSIGPTEVDQNPAMMSLQELMDEGRVPVFVVPTPQLHIHYDTEPKVIASATRSYVRDAKRYLKLVKGGDRDEVKCLFIGATLSGVAREWYDHWTTVQEHFTFEELTNVLLTHFAPGNQSQVAAMYQELHAGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S772</accession>
    <name>A0ABQ5S772_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-ketoacyl synthase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="31058" checksum="B6FCCBB7D5219DFE" modified="2025-10-08" version="1" fragment="single">MQSQSRSLCGRVGWAFTQLLSHQRICGLQSSARALIDHQGLSTSSDDGPLSSKAAGTTTKLQSVLSIQQGLNTFAAATVTFGPSLREAEEDSAALGPAAQHAAQVALLALRGALSAHRPPSAVVALPSLGLPSLAVQALTEALAEQLGPGTALVGCSSRLRLPDEWRAVAGTGTATAASAASRGPFVAEAAPAGSESLSSQSLSSPSHRYYITLVAAHLPQHEVHAVRCDTSSLPRLPHLAEVLQGRRPPAFLLLASSDSLALELLARLENLFPGSGAGAGVAAQQTARDRRQLLVGPSGAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7I5</accession>
    <name>A0ABQ5S7I5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003882">
        <property type="entry name" value="Pistil_extensin"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01218">
        <property type="entry name" value="PSTLEXTENSIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="380"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="462"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="493"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="788"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1060" mass="108029" checksum="DA6A7C2FC6F44F7D" modified="2025-10-08" version="1" fragment="single">MGPLAWPKIYGVPCCLQLWIALRSCATLRRCTARSPAASLPLWCLFLTFVALALISLPLSAAPAAPPAIPLPQPLPNGAPPNSVCPPTLADCPGDITVYYAVDAESKPPPHNASAANFSALFGKPTFTGPCLDGPPTTPADQSSLWSLGSGDSTNSTGGPSRTKLTVKFNSSLYALEVQLTVASSRGNAVSGVDIRLASGRLLKVSCPVDSVARCTRSEMAYTYVCSLLDETTLMASNLVQGAQLDIDRWAVIDAVALVGFKPLKNLSVDYDDDDYGDYDYGGGTRTLPPSRAPSRFLPRPPKRAPPPTSPPPPLPQPPPTPRAPPQPLCIALPSLCTIVGSSWPPLRSPPPRAKRPRKPPSSPVYSKPIPWPPSPSPYPVQLLRPALPPSLLRSASPPPSARTPPPSLPSSSPLSPPSPSQALSQPPPPPPFFRQQPHLLPPSPFPQRPSFFLPLPPLNPLIPNSISLLSQIPPSSGLTPSPSASSPLLSSPLPPSPLPPTPPSPLPPSPPSPLPPSLPSEPAPSSASAPSSVPPLPPTPVPGRSSTPASSVTRSPRPQSSAPRTPAPPILSPPSSASPPPHTLPPPTTPVRPLPSPPKPPSSSPLPSPPPSPSPLPLPSPPPPSPSPSPSPPPPSPLPLPPPPSPSPPSPSPLPPPSPSTPSPSTLPPPSPSPSPSPPPPSPSPSPLPPPSPSPSPSPPSPPSPSPSPKPLPPPPSPSPPSPPSPSPLPPPSPSPSPPSPPPPSLPSPSPIPPLPPSSAANPSTALWYAPTPGPTLQPDAPSRFPAIPPPTVPPPSPPQPPPPQPIPTPPPQPPPPRPAHPPPPPPPQSPPPPPPPQPPSPRPAQPSPPPPPQPPPRPPPRLSPPPPSPQPSPPPPSPQPSPPPPPQPTPPPPPRPGLLPSPLPFPRPPPPPVPPPYVLLGIPSSLVNESNPSGYLSISRDQSVPAQDALTDVNATSFPGATAVKVLGWNVTAPLRLISKNPTSEPTRCNADLTAAVTANLGTRLGIDAAVVNVTCTTSAAVAVNAAAGVGVSRRLASAATTLAIANLAAAAAAANIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7V1</accession>
    <name>A0ABQ5S7V1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_009613</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family. CPR flippase (TC 3.A.1.211) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056788">
        <property type="entry name" value="ABCA2/9/11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF205">
        <property type="entry name" value="ABC TRANSPORTER A FAMILY MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25158">
        <property type="entry name" value="ABCA11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="453"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="582"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1085"/>
            <end position="1131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI66002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1131" mass="122813" checksum="8F457894B382B6E4" modified="2025-10-08" version="1">MGALASSWEALLDFLFSFRNVQQFFVLYRKNALIAWRNRRATILRILAPLLFMILALVIDRAIQANDGSSSAFDDVQSPDSELINAIPQCSEDIYIGGKKGCVEVLYQPSPDTAVDAIMQAVRTNNPKPISVKGFVNRSAIQSFLLDNPESVLGAVHFEKSGSALEGFIIQVNTTSKFFKGTYQHPNKFMQLPLQAAVHREIARYQLKNSRLPNASGLAAGLQLEAALKEFAHPTISTVTVLGQVLGPFVFAACMFSFVIQIGVVVAEKELGLKQALRTMGMSDSAYWLSWGAWEVTLAFIVAHSICIYGLILQFDPFLHNNYGLLFFLFFLFQLAMSSLALVLAAFIRRTQVAVYVGFSVFIVGWIMQAVVLSDVPYSPDYYYTANKAITIVFSLFPWDLLAKGFKDLGAATGGNNPGINWNERSSYCSNIEDVDKQPSYDRRTTYKDFQCVMSLNTIYGILLALWGGYFVLAIYFDNIVPNEFGVRKPILYFLYPSYWFPGCGRVQNKLKTVRESTNPGRTARIGDRPTPPAVAELDEDVKAEEDKMKALLQHRTDSGPGAMSLQSVGGAGGVQTPTNAVEVYGLTKLYKGSNGCFGSSIKCCGCCDCCSCEKTEDFWAIKGSWFSIEQGQLFCLLGPNGAGKTTTINCLTGAIPPTGGEALVYEEPISHPGGLDRIRAQMGVCPQFDILWNELNGSEHLSIYGHVKGLPRRKVASDVATLLEKVKLTYAADQRAGAYSGGMKRRLSVAIALLGDPRIVYLDEPTTGMDPISRRYVWDIIQESKAGRAIILTTHSMEEADILGDRIAIMARGKLRCVGTSLRLKQRFGSGYTLSVSVVSVAKAASSGRAGRAPGHQGLATPPAAAAAAGVTEVAEPTTPAAVEQRVAGVKRFFQDRLGLAPVDESKAYMHYLISRDKEPQLNSFLAELEARRDSLGITDVQLSLTSLEEVFLNIARKAEMEAATASGHASVKHTMDDGTRLQIPMGAELVAHPVTKVMYQVRWGTDEAGRLVILDTSVAPPPATSAPPPVMEPPSNGTTMPYDYGNGNGRIPPVPGAFVPASMADMPLAQPVPSPPLVAAGFQQAGGRDSGGMRPSLVSSWAERPAPDMTNMPAPTYPGYNPSPVQSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S882</accession>
    <name>A0ABQ5S882_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009609</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16655">
        <property type="entry name" value="RING-Ubox_WDSUB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052085">
        <property type="entry name" value="WD-SAM-U-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46573">
        <property type="entry name" value="WD REPEAT, SAM AND U-BOX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46573:SF1">
        <property type="entry name" value="WD REPEAT, SAM AND U-BOX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U-box" evidence="2">
        <location>
            <begin position="30"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="364" mass="36696" checksum="1F18B08ABEE1E706" modified="2025-10-08" version="1">MEASILAVAPGVVNNTCIDTDTHTHTQMHLRSPHPRCPITQSLMREPAQASSGVTYERPAIMQWLDRRRVDPVTHVPLKRHRLAPNLNLRHMIEAWVQDRVEARRRLAQRLREEVSAEVAALAAVTAEVAEAEKAAAAAAGAVANAITGSAESVSSAEPDGGNPDVDSAAVTVVPAVAVAAAAAVAVAPTVLAANSSMPDAAGDAQLSLAGLPCRSATAAEARDAPACSGLCESGEDDSMLSELHGGNSCGDQAAVQVEKVEALALAAARARIGRRRGGCRYRNGPGPPAPATAGGGGGGGGGGGGFATFRNHRSSSSGGGGSGGGGQRMSYLAARGDDDDANCDGDYATGATSAGSVESPSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8G3</accession>
    <name>A0ABQ5S8G3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009315</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="35415" checksum="CD9B194201EF1E48" modified="2025-10-08" version="1">MALIQTSFLRFGPLTSCKGRARLAAASRTRQPFVLRTAFLSEPEVQTSHLLLCKSYRLAIAGVLASGLVWLSPSPVEADMQTVSPQMVTQMARPLPKQTVDKGKIWSIFIGGAVLLFGITVAFENNEKFFPAITRANRAFRTAAKEFEEMEERQKKEEELLRLRQVQYDEEQRQLSVVQLGMQEARTKVLGTGAAAAPVAAAEPESSSSHPVIQASTSTNTSSSAGGDAAPYVVVEDVVSGIGAVAWPPTAAAATAAGSAVASTSVHVEEVPTQAASAANVGAPLEPPLLSDTRAVASADDAELMTRVARLQGFVSGTISGSKSALEAYRTQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8S1</accession>
    <name>A0ABQ5S8S1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="403" mass="43421" checksum="8A2A13892D07D124" modified="2025-10-08" version="1" fragment="single">MNPFLQLMQRNEEELKDLNPAPRRHHQGRFPDEVLRNALDKHYKYKTFVSLGNARPICSVAGIGMARLQFGDGEYAQSVHEMQRLPINLRTERLPEIEAPVQVTRTLLRTPSLNPMAGAPCRNNVAAVSAFSLNAALSSPSVTSALTPSASTTSSDLTMLASRPSVSFRETGSNLAAILAVTEDNMGEAEVAERWAPIDSRPSEAGSSSRDLGISWPRRVSRRSLGRSATASRALSITGEGDAPHGSANDSLQRRPSLPPPELAAVMSLQQQLQQWLQPHSQLGSSSPSFPRQESTGADRVGSRPSLPLQTQQPLQLQRPHAPPPPQQISPQMLKQHQQQGCVGSPSSTQSGESCTSPLSTMSIADYLRSCSAEAAAAATPCKLPTSLQPYQPALQPPGRAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9G0</accession>
    <name>A0ABQ5S9G0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="166" mass="18652" checksum="FD03F6E453A0D5E0" modified="2025-10-08" version="1">MDFVVALPKTEGGYDVVFVMVDHLTKMVHLAPCTSSCTAEQTARLFFDNVVRLHGVPKNVISDRGGQFTSKFWDALCKLVGMRVNLSTAYHPQTDGQTERTNRTLGDMLRNYAGRTPLVWDTYLTAAEFTLNNAVNRSTGKSPFFLNYGFHPALPVWCEWCKALIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9I0</accession>
    <name>A0ABQ5S9I0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4350 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148:SF14">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148">
        <property type="entry name" value="NF-KAPPA-B-REPRESSING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="44374" checksum="F28145F78F4350C9" modified="2025-10-08" version="1">MDCKRGLRIANMALALGIFVAMFLPIGHAVSSIIPNVTIYMSPSNGVLDLTWDASPYNLERQLRDLGLYVTAAYDGQPSLTRDNIPNAYVIPYQNGDTFYVSAEDMREVTSYVASGGLVILLDAVTGDGAAARAFVSQALNLEGDWYLCKRASKPKVSNKRFVLSNLGDGVILSDWASAFLRGADPATAVSVSREAGAPWPRRLEDARFVNAHTLCLHEDAALITRPLYTVDADDMQVAAQAFGKVGVPGAVVWLGYSWKAGPQEQWGALINKIITDFAEHAYKVPTEEDAAWEPRDLGDQVVEEVASARGADLTDAVRLLLEGPGIYPPPPPPQLPPNNGHHNGDNNSNGNGNNNNGNGNNNNGNGNNNNGNGNNNNGNGNNNNGNGNNSNGNGQKPIEVPRFVRRLRAAFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9I1</accession>
    <name>A0ABQ5S9I1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="189" mass="20603" checksum="51E3BD4D51CB1A90" modified="2025-10-08" version="1" fragment="single">PRAATASGARGNDAQQRKEQHQQQGTTAAEDEGEGEEQRQQPKRSNCKGASERLIDMERRVKEQDLEASEGHRIDGRLWGAFHPAEAAVGEAEAIMQAMETPADEQMQTEDDIDDIEMERLRRHDTPDGSDMERQHRAAAVGLTSLGLLAGSSQGPPTAGKSSKATSGSDESSLGRETEDEVMAKETYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9Q7</accession>
    <name>A0ABQ5S9Q7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF2">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="233"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="887"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1046"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1303"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1431"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="911"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="941"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1056"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1079"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1431"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1461"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1877" mass="193951" checksum="8C3B0512CDB2B7FC" modified="2025-10-08" version="1" fragment="single">MADDIKVINANDLLKGVKLTFHGQAPSDAAPADVHNSEGTGPRTKQKTQGGPPGDQKIAVPSIAVGPPPSANGADADMGTAAAAAAAAAAAGRSMPDGAPVPSGSSLLEGIAESLSEQYHSGEWVVPPGQPNDWRFMLPVCRAALVAWFNPASTLAERLALTEAIGRAFGLDDHALEQAVAESLITNVQIPYQLAVQHAALNGNTFSVGPGSFPGDQAGRAAYLEFRREAMVEAEQLLQQQLGLPEFNGLLQVEVVGATGLKVNRPKSGSKQLLPYTMVWLNDRSRNRRGEKLFTQAATDPERPVWIQQLPPMTVTSPESELVVQVHGALDRNRPARVDPVLGRVVVRLAGLTPGLSQQVDLRLYGSKREVPRGRGGGGGGFLCRCFGDEGAAWKEPDTATVAERTASEDPLDDVDGGKLVFDPSRPPTSGVKKPPGGGLEEVGLLTLIVRYLPERRERLRRLAERSAPGPAGPNNYHTHNRHQRNHSNTDIPPLGTDQLPMYDDTAGGLPAAGAGASISSLPTLAEALRGQLETLHAPLPYAVRRVRRLDSRKVDPLAIMDPGMDFHVTFRKLAAALQALAADGLKGAAALAAVPESGIPLTALGSLGQFLEDSGLETPLERFLPRALGPTGLTLLLLFAKLFRVRPATQQIALLQALLAQGSWMPGSRVYLELIQRLWEPVLLWNSAGRLTLIETEGLIGVNAIFLHRACPLLANQYDKLSGEEAVPCLVLLVEMVGWAVTGDTRRRASGAASAAAAGGAAATGPSAASLHLLIDHMQRAAFATAASISALRPTHDSLASDVEALTAVAKLVLDHLERDMRLQAEIPGLRPLTRTNSRVRYDALADSLETLFMYRPGSSKAASTALWPLEVMVTRLHSFMLRHQLARQPHSEGTPTRSGPTGRRRLSGDDTSSMTGSSLRSRASGVRDTYGDNTNGGDSSSVSPVSVSLARASHNASRPRRRSDGGGATDPRVSGVSGGGAAGNSRRGRSSVDDDDDEDSSGGGGEPMVVLFDLEAAMAEAMAEWAELGGEDLKRQLLRLLERDPLWPPPPATHGSNANGPNQSQVTARPHGAESRAGVSSQSAAGISGGGRVGRALVGKALLSSGAGESAVELFRMLQVYLDVSVERALGGGEQRPALMGPTICTSVVSCVRMYLYHCMRAWEEVITLRVPSPEGATAAGHRHARTISDRFLNALGVSPGAGLAALPSQAAVAATAASAGGGGGLLGSFAVALGAAGSSQLHANSPAVGRTARGLRPHAAPVMPEYGVMPAAAPGAAAMVAALSTAMTPGAGLNATAAGSVSGAGGPHVTGGASQPAAVTPPPGVGPKKGHRRAATAVDADTLAMSHTVSSQPGGGGGGGGGGTLTAAAAAAALPPSTALALASEPGLVACGPLVLIRNTIAAVSEGAEALEGTLGLLGPRPPIVTSKAATPAGTAANTTASRQTPAAADISKSQQKPSSVAVSPASTSSPPPPAFLSRATAELRHALNLASEQCLTSYHLALRGSVLRGLIAAFDSEGMRPPSRAALESVLSALHDELLHVAGGVRQVAVAGAMVHSCWDAAVRCVQALALHQGGFRPLQEQEADVLREFLVSLQDMFGEVFSEHLTPKAKAAAASAGGAALSSGPHSSSTLSGAAPCSLPAVLKTADSRAAGTAYPSALLQCVAASTEDLVNMYSAQMSCLQKMDAPYTDEDGPGIGPGGHQVSLLDLLRLLRQRRRTDPAALTFVSEQLKLVASNATQVLFGLRANERLVATAACYLAPPLAAIGPSAPGSGLSLPLGPMGPGGFRDGCLYVTSRVLGFSTMLAGDLKGTTDVTSCVTLKAVREVAKGESNDTLLVLTHGGDLYQFCGFAPGERDRLCALINGQPFGARIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9R8</accession>
    <name>A0ABQ5S9R8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_010191</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="11"/>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF63">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI66399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="325" mass="37103" checksum="EEA61216804B1AA8" modified="2025-10-08" version="1">MAIDRAPINTRIWSDGMHARFKQGIVSRIVAMITLFIYTGWMHILLALMVLAICRRTWAIIALISLYATLLLPPKPVLWGPFCRSKVFKTWREYFSFSYLFEKVLDPSKKYIFAEFPHGVFPMGPLLGATECQAMFPGFHIYGLAANVVFSVPFWRHFIAWLGSVPATTPHFKRVLKRGSVAVIVGGIAEMYMQDDRKEQIMLRDRKGFVRVAVEEGVDGGIVPVYHFGNSRVLDFGPQALSSLSRSMRAALGFLHGTWYLPLPRPRPIFMVVGQPIPVPRVPRDDPKYEEVINEVHKHVCDALQKLYDNHKTEYGWADRPLVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAE1</accession>
    <name>A0ABQ5SAE1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">T-complex protein 1 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_010231</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03337">
        <property type="entry name" value="TCP1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012719">
        <property type="entry name" value="Chap_CCT_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02344">
        <property type="entry name" value="chap_CCT_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="526"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="542"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI66433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="558" mass="60841" checksum="4500BE7DD9D4D96E" modified="2025-10-08" version="1">MQVPVTVLNASTKRETGKRAQFANIAAAKAVADIIRTTLGPRSMLKMLLDPNGGIVLTNDGHAILREIDVSHPAAKSMIQLSRTQDEEVGDGTTSVIILAGEMLQMAEPFLEKNIHPTVIVRGFARALEDSVTIIDRLAFPIDTGNREQMLNVVNSCIGTKFTHRFGTLMAELALDAVQTVTVDLGGGSKEIDIKNYAKVEKIPGGTIEDCRVLKGVMFNKDVVVPGRMRRRIVNPRILLLDCPLEYKKGENQTNVELTKEEDWAALLKQEEEQIQRQCEQILAFKPDVVITEKGLSDLAAHYFTKAGVSAVRRLRKTDNNRIARACGATIVNRTDEIRDSDIGTGAGLFEVVKIGDEFFTFVVDCKSPKACTVLLRGATKDILNEVERNLTDAMGVARNICLDPRLVPGGGACEMAISHGLAERATSVKGVEQWPFKAVGVAMEVIPRTLAQNCGANVIRTLTKLRAKHAESPGCSWGIDGETGEVKDMKQLGVWEPYQVKAQTIKTAIESAQMLLRIDDIVSGLSKKDRGGAPGPGRGAPEVEDHDNVESERMLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBJ1</accession>
    <name>A0ABQ5SBJ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein MIS12 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010932</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000047">
        <property type="protein sequence ID" value="GLI66905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008685">
        <property type="entry name" value="Centromere_Mis12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05859">
        <property type="entry name" value="Mis12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="24063" checksum="DEA12E69F68EFD06" modified="2025-10-08" version="1" fragment="single">MAFQQPEPGQTFADDVHNVVLDYCADGVDAFLGCVGPALHRAGASLEDERLLLEGSQRLYDSLVAKASSSLQLFEKLCKTMIFQVPPPADIPKMKPVSLPEGMSDGFGSDFQSEEQLIVQIASMRKEICKVDRRCMSLHKELQRVDNAMVACGDTSSVRATASTATSIKDTILAISEAVENLHVMLEKASRLQSGAHQGIENPVATLNEALRRVPSDKVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBL1</accession>
    <name>A0ABQ5SBL1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="365" mass="39962" checksum="4CFA955A6DF9B1E8" modified="2025-10-08" version="1">MLRKSAQAVLQAERTGLVQTCSVAQTAFTRQFGAPAHHDSPTTPLTPIMPGIVALPRQVISTALSLTGKAVAGAATSNYVKDLVSSFADKAILSESLAKVDEVDVPFWAYWLSVGGYSSPAGYKKFADAAKAKVLGLEPQQVTDLAVAFHKVNYYDKDLFTGIAANISSNFTKYETEQLLKVLGAFLEFGHYDLAAFDDIADSITYCNHYLAPIKASPVELANAFAAYQKFEHERGDLFIALARGFNEASLSKLDADIRKAVVLKALRAFHAFQFWPEATEALLYAAKSSLASYSADELKEVEKFQAMLEDAHGGELRVFKEGDDVDSVHWYGHHTPAPSSYQLYVFRDALVPKQYSPAGMRPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBZ1</accession>
    <name>A0ABQ5SBZ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_011079</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI66983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="53"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="595"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="724"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="624"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="736"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="760"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="772"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI66983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="796" mass="80330" checksum="B1F218A88ACAC69F" modified="2025-10-08" version="1">MSEGSSPAGHCSAEQYASLLQALHGDGAAATELCKIFTTIDRTEEVAHGLNTVFMVLSGALVFIMHGGFAMLEAGAIRSKNAMNILLQTVLDGAASAVMWYLVGFGFAYGIGDKPNGFIGDALFGLARYSSHHTITATGDWTSWFFQWAFCATAATIPAGAVAERFNFNAYLGYSVFLGGFVYPVVVHWVWCPTGWLGYSTNTPLLGAGMIDFAGSGVVHMTGGLAGLIGAWMVGPRMGRFDANGQAVHMPGHSAILVVLGTVLLWFGWYGFNPGSALVADTFSSAMIAGRAAVTSTLSGAAGGLSCLALAVIRHKAWDLTSLCNGVLVGLVSVTAGCTVYEPWAALLCGSLGAVLFEAACAVLLRLRIDDVVSAGPMHGICGAWGVLFVGLLAKEEYVRQTFADRDTYPYGLFYAGGGKLLASQVIGVLAIAGWVAGTMGPFFAAFKFARSLRISAEDESMGLDASKHGGSAYNIPMQRYIGGFGEGPAAGENSNRGVFVTPPYTNSGIVGGGVGVGCIGSGRAVGDAIMRGVTFGGSTFIVNNTGGVGTVSGGNVNITTNGSAFVTPFDGLNGILSTRPISSPAVSAVAGHTSGGPLMGTGGTDSSVGSEGPRIFCQGPVGRQQQVDGTSPSCSAPTLAVAADDALQPPSQPGKQAVPSVSEDPAVDSGGGHNGDVNTTKVDVVCSGASGCTAAAAAAASRATTAGGSSALTGDTAAISARSDRLNDRRIGSGASPSTSTTADAIGSIITSETTEAGTAGGGGGGGAQDSHGQSTSNSSCHGVTVLNIEVDGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC69</accession>
    <name>A0ABQ5SC69_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019265">
        <property type="entry name" value="RTRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15924">
        <property type="entry name" value="CLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10036">
        <property type="entry name" value="RLL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="274" mass="29258" checksum="349E7859B67117D7" modified="2025-10-08" version="1">MALLNFQLKALQYPHEVDITNTELVRRLVIWLENQKIREYKIEDRKALSDIQSPGWDVAFKKYLQDLECPLPPGDMTAALHWLVAFAVNLEYEDNADEVAAVSSPPPAAAAAAGSEKQPATSGTASGGQDDQPFTDLEDPAVVAAVLQLISAARIETTKEGGGASSGATSSSSSSTSFIVDRLMAAAQRCVCEVGPALKTGVWSPGKPPRQALASVPLGFGLKGEALSTAATALRLLYIQDLRRLQSQIDHAVVEMQEYTANPRTDSSLGKVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDM0</accession>
    <name>A0ABQ5SDM0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_012188</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="848"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="468"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="543"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="643"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="478"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="656"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1040" mass="103899" checksum="D895D20378AD26F6" modified="2025-10-08" version="1" fragment="single">GHGAGSGSRDAGGDHRAAVFITTSDKYPRSRAQAGSSGHGLLFSHAVSSPFSEALASQMAQNVSQRSAFGSLGSAGAAGMGFLGRTAAGGSHSNSAAAWISAASGPFSTNPSILASDQAGELMPPSAGLYRSCDYGDVHSSGSPADSFAAVAFGASLSGPCIEENGDVSSFANNFGSTFNGSETLAGMFATSCHMSAASGGGVFAVTKAGCGILPSHAAKLGPTTSCGVDTPASVAAEVGTPAGPRHSVGVRESMSKFRRGFSGGLVTETAALRSTRGAYLNTHGCDGSGVANTSGGSRPGITLVPSVGGGAGSFSGRTASPVGTLGVLPRMLDLAIKVSVRTLRTRGSASRLGQLSGGSGSAAAILTGDGGGGGGAALELGSGVSFTMVDPGPNKSGLSGCGGARSGGGVCSAGGESVSSGPGSPAMAAGRMASLGASSSKAGMQGVRQPSFSRFMQRADTCASGVLDTIRSSGPSSRGGGGGIRGSRGGDNDNGGGSGDVGGSLGSDGGSGGGCGEWLTSQSPSGERITFMPQPATIAPTAASLAPKPLLSETPTSPDGGSAEENSVFGGAALFAAAAAAAAAAEEARMMHPDGGVELPPQASAWGWRASHRSQGTFTAAGFVSPKLGGSLAFTGRALSSVQEGSTGAPEGPITAPPLPRIPPQSPTSPMRPSVGGHGAGGSAVMEIWHEVVVSCMRHPNTGEQLIMVTQHDVSARVWAEQQLARVMEAEHALLEAIFPAHVLEHIAIMAAAAGGDEADTECGGGSLPQRPTLKGLTAAAYEAAEAAGLTTTPSMLPANALGGGPVPCLAARAVSRRREHQPPEPPVLHITGDTFLHLATSHSALTVLFCDIQGFTTMCNSVKPATVMSFLNDLFTRLDAMLDAFGVYKVETIGDCYMVAGGLMKVDEETGAVTVRSDDVDPQHAHRTVQFAKALLHAASAVRLPSTGEPVRLRVGIHSGPAMSGVVGTRMPRFCLFGDTINTASRMESTGMPGAIHVSQATRDLTPNEPWEPTGGVEAKGKGLLQTFLLRPLLLEPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE90</accession>
    <name>A0ABQ5SE90_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="172" mass="18848" checksum="C34378A1C3A67788" modified="2025-10-08" version="1">MCQIKFDFTFNAGIAFKYNTILFILLSLAVAASTVFIGGLSALQNECHDDAKYSRNTNDLLGVDGFSAFALTCKKIYRFYWFIVSFEYVVAAIGLYAVLVEGKLAVLKGSVQGLFIVSTLLFIYTSNSFLNVLEVPLFQSETLRDRSKTAVAGAIITATLNALTVFATGYQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEI3</accession>
    <name>A0ABQ5SEI3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="213" mass="23222" checksum="5CB0ACEB934C7A6C" modified="2025-10-08" version="1">MLHVTDENAVTSNKTAKPGVGDGLCGVKDTLRPGFGKPLSKPAMAKSQSQQKTRTALCDLTNVMKGMPVSSNKLAKSTARAVPELPVEHLAGKGWQVMEEERLQQREAELQQRLDRIAASASTWTTSHLYQEAISLRPGFLQDSSDDETDSDGSFDERFSESPVIVQFLAPRCTQGVTMPQPGSGFNVELYNLLPDVPAIPIDLGFCWTDDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEV9</accession>
    <name>A0ABQ5SEV9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_012201</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase delta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020781">
        <property type="entry name" value="ATPase_OSCP/d_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="ATP SYNTHASE DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00389">
        <property type="entry name" value="ATPASE_DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="230" mass="25605" checksum="20F0DCADB0352F70" modified="2025-10-08" version="1">MLARAACLLACRAEQLQLSKQLVRCFAAAASAPKTDYKLPVAPLQLSGTSGAIATLAWQVAAKENVLANVQDELYQLVEVFKTYPEIRRLATDPFLPVAFRQKVVCDMCAAKEVTEVTKRLMEALAEENSLSAIVQVTLAYEELMLAHKKEVHCTVVTAQPLDDAERAVFTKQAQAFVEPGFKLVMKEKVDRKLLGGFVLEFEDRLVDMSKAKKLEEFNNLVTKLENDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF98</accession>
    <name>A0ABQ5SF98_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_012404</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0014 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005226">
        <property type="entry name" value="UPF0014_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30028:SF0">
        <property type="entry name" value="PROTEIN ALUMINUM SENSITIVE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30028">
        <property type="entry name" value="UPF0014 INNER MEMBRANE PROTEIN YBBM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03649">
        <property type="entry name" value="UPF0014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="341"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="479"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="583"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="359"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="587"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="626" mass="65508" checksum="86A4EC81CA62BB6A" modified="2025-10-08" version="1">MFRGIRFRTYMQIFRYNFKTFPYAVIIYHRIFDSRCHRLVMAAHLCRHRRGTPYPPKLVIASIRMVVQLSLLGYILVPIFTYDKWWLVLLYGVFMLLIASLEAVQRPSYTFQGVLANTLFAMATSSGLLLSYMVLVVLGLRPVWEAQYIIPLLGMLLGNATSAVSVGLSTVLEDLASNRAVIEQFLALGANRMEATDEAVRRALKVSMTPLLNQMSVMGIVSIPGMMTGQILAGGSPTQAARYQMVIIFIVVAATGLASVSSIYLAVLHVVDSTHTFCAERLIKKQQQRAGAGGKWAEWVAAARRGAAVAGRKVEALGRCLGAACCCCFCPPPAPRRRTRTHTIGGGPVDGPADALLPAAGSDSATSTSDETAVAQSGRASAFTSIPRYRSHWRPTAWIWRTGRTFFASPTRRGAAAHHDYPHSAGSSASSVLLGAAAAADGLAEGPGSCIMSPLSGRVPGAAGSSITAKAFWDERMSDREVEGSGRGDGGVYDAEEGGDGNATGLGLGAADQAQQALVGSGPAAAAAPQVGGGWSGASLTHGSSMLSVSVSTVLAVGGWLGSQASAGWGAAWDAVGKWRSPARNVGATGPAPGAASGQRAGTSAHDGYQPLLARTEGGTDGCASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFK4</accession>
    <name>A0ABQ5SFK4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="2">2.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012521</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="7">
        <location>
            <begin position="2"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="8">
        <location>
            <begin position="370"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="8">
        <location>
            <begin position="541"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="698" mass="78043" checksum="99701D70DD51F6F7" modified="2025-10-08" version="1">MCGIVGYYTYNVRRDLRFILDCLFNGLKRLEYRGYDSAGIAFDAVDAYPLAQHHKEGHQVIAEENGILEFTGPMIIKEVGKVDALERMTYDFISRDQLDLKREFRSQVGIAHTRWATHGPPSAVNSHPIPSDAEGQFVVVHNGIITNYNLLKAFLIKHGETFSTETDTEVIPKLCKFVYDRLSEKVPFPKLVMEVLKKLEGAYAVLVKSTHYPGELVACKRGSPMLLGIKEEPGARRASFSRLQDAADTKWRREAIECFIASDASAVIEYTKRVIVLEDNDVLHLCGGGYGIYNTSGENVEEAVPRVLLTLQLEVEQIMKGGYDHFMQKEIHEQPESLLQTMRGRVQFQRPAVGNPYLTQRIKLGGLVEHGATIRRCRRILLVACGTSYHACLATRQTLEEMCEVPVSLELASDFLDRRCPIFRDDTCLFLSQSGETADTLRALEYAKAHGALCVGVTNTVGSAISRMTHCGVHLNAGYEIGVASTKAYTSQIIAITMMALQLAEDSIAKRERRDTVIDELGQLPGKVRRVLMLDSAMRDLAEHLRSVNSLLFFGRGYNYATVLEAALKVKEVALIHSEGMLAGEMKHGPLALVDKHMPIVLIATRDGMYKKMESVIQQLLARDAQLYIVCNENDEAMKQYEDKGCKLIQVPETVDCLQPVINIVPLQLLSYHLTVLRGLNVDQPRNLAKSVTVSEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFL1</accession>
    <name>A0ABQ5SFL1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046362">
        <property type="entry name" value="Zw10/DSL1_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205">
        <property type="entry name" value="CENTROMERE/KINETOCHORE PROTEIN ZW10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205:SF0">
        <property type="entry name" value="CENTROMERE_KINETOCHORE PROTEIN ZW10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="854" mass="89317" checksum="D8D9D6A69BB7904C" modified="2025-10-08" version="1">MSGSWGVAAVKAAWSTGRNLDSTNPVSIQESIDQLDQIQVQVRIRLFRQLQRLATQPGEGLGQALSQLEAQADKVIPDSHEASMLPHIGTYREIISRIQELTREVSRKQAAEDVQKQAQEFDDRLAAGSLSDAAYCCVQLEKMAQDAEEELAEQAARRQQQLAEEGEGEAGAARDGSGDGGGVEGVGEGQHAGGLPRVTVESVALLQEASERCSTKLKETLEACCSEAVIVDTQGSRVVVRAVLRGGSVRVPDLFSALQVLGLQQLHLRRITDRLVSGALEPMLTSPCCVTITAATEPGGAATLAWTPAPPGSTGMTSGAGGTARLLRPHGSGGGGGGGGGPCDPEHCCQHLLRLLATWFLEFDEGLMELFGGPFWRAAADCYIAHVARPFIAANPEDVEGCEAVVAAAADLEAMAGSLNITNEADPYLAPAVETLARHALSSRQEKYLERARQLLVEGSGGPHAAPGGGGSGGDGAGSVWETVVAGQALQVDEEYYRRLVAGEMQDWEAQDPPSGCRTDGPVLATGRYLITRRTSDLVQLLTGLMQDACAGSRALSRALLSAVSSIALMARTLPPLAAAATAAGAATAAAAAEQSLAAVPQLGLLAVTDLQHLATTLMVLPQAYGAALEVATGEEVGRMLLGEALQLRAAARARMQAQLRIQYGSLDEILAGLDGLHTVGHADAKAGQRHRRVIHQLLHSLGRLGRAASEVLTPDDAVAMGAAVLNYVGGQIVAAVMAKGDIGHDESVELAELLAPLADGAVDAWLPAASATGPDLSGIPLGVVRSALEARGSQIRKLQCVLQVLRSDTLAHIEQMWDSGDLAMLAAPELEHLLLAISENSPGRRALLEKLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG41</accession>
    <name>A0ABQ5SG41_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="110" mass="11580" checksum="FC6164C97699FF36" modified="2025-10-08" version="1" fragment="single">NLQVSQDQVDQTITLRVTNLTFRYTVTPDTGPIDFAAADVVLICNVTARAHVPRDMRVLPLASLSYPYIEGSLTIVTQQHAGIGAPSGPGAPPMPFMTPPTPAGSPRPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGJ7</accession>
    <name>A0ABQ5SGJ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013637</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033337">
        <property type="entry name" value="TORTIFOLIA1/SINE1-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057600">
        <property type="entry name" value="TORTIFOLIA1/SINE1-2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057599">
        <property type="entry name" value="TORTIFOLIA1/TORL1-2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31355">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN TORTIFOLIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31355:SF7">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN TORTIFOLIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24713">
        <property type="entry name" value="TOR1L1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24714">
        <property type="entry name" value="TOR1L1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TORTIFOLIA1/SINE1-2 N-terminal" evidence="3">
        <location>
            <begin position="20"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="TORTIFOLIA1/TORL1-2 C-terminal" evidence="2">
        <location>
            <begin position="770"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="696"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="897"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="696"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="906"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1056" mass="111172" checksum="E26E2548CF05BE27" modified="2025-10-08" version="1">MPPRMVPGPAGNTASKMIKKERIIQALSRMNDRDTQKAAAEDLLSILLNMDQEGLFIIVSCLCTTGSEHKVYARKECARAIGIIASEACPLREQALQQPHLGKLLGQLRRSLQDPDSGVREASSEALALVAQGLAYLDANGAQGSPNNPVVKLVFDCLAEQKKDLNAAACAALGMAAPYLGNLDTALSRELVRKLNSPSFQAPAALLAALARSDPTTGEPVGLMKSGPAAFMPFLSALVGQPAPPSVGASTSNLGSGVGAALSRPDWPVRMAAADMLRSTALLLGPLMEHDGCWTRGDAKSITGRAIRALETCKFDKVRDVRDVARNALAVLEELHAYGSGGGTLQGWPEHIARKLYGSDLRPPVATSTFVLPSFSEGAVPPGRKSPGRRSTTSPGRRPRSAQSQGSHAAVSSPPPPACSPNAPERRFSPMLARGGSVSERFRAAHRDGNVVSVHYLGAVASEPLLGSAGGEADNEVAQLQPQLHDEELAEPPMFRVAAQDDTLPDVAPGNRRLSRQASKASSENAKAAERLHSLEPSGALQGARVDFGASDELLRNMTLEDDATAVLDSNGPPTFSPHKIAEPPSTAELTAWATAAPFQPADFLFSAAGVSGTAPAPTAATPPPVPEVRSSTAAGPSVSIPVQEWLAAQQRLRSLEVQQRQLLESFNLMSEQNKQTIAGLQAKVRTLETALEAAQSQMAAQNQQQQEQDQPPEVDAEVSWMSRTIRPGSPIIPLLAPSRTTADGSAGPTPERTASGGTAAPVVATSTADLNDVRQAYREVLASGRLSDMPLLRLMQRTGPVWGDLGSELSAQLLAAFMASLQQARADGPLSRIMPWLWRLADEQNTVFEAPVEMRVPLLAALSNSQAAVTDPQLYARVLLLINTLRTHWALPDAQSPASNHRLYPQPLQQSHGQLPPSPPPPLRQSSPIVSSTQGASGGDTPQELSFGAGAARFVPTAVTTAAAPAAVVGGSNIGIGSESSRAQLVPTLTLIESQSPAKEASGMRTGTPRVAAVRSSQAGRSPSINTLMGQWSDLQRDFAAMSSPSQRPISRGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGY5</accession>
    <name>A0ABQ5SGY5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="18273" checksum="1654BCEA0A0D470A" modified="2025-10-08" version="1" fragment="single">MEVLSDPQVVTTHGSEGNGTPIQGGADASGYGTRSGRLLQPPPLRPSSHHHHQQQAVWMDEHVFLYRLVSGQCSASFGVHCARLAGLDEAVCRRALEVISAQQSGGLVSRSLRVLSSPQVILSVRRLRQLQASSTLDAPERDAALRSLLQRDAEEAQAAAGAGAVGMYRGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH60</accession>
    <name>A0ABQ5SH60_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methanethiol oxidase</fullName>
            <ecNumber evidence="3">1.8.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_013383</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>methanethiol + O2 + H2O = hydrogen sulfide + formaldehyde + H2O2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16007"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="EC" id="1.8.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Organosulfur degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the selenium-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.4" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008826">
        <property type="entry name" value="Se-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300:SF0">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05694">
        <property type="entry name" value="SBP56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75011">
        <property type="entry name" value="3-carboxy-cis,cis-mucoante lactonizing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0711">Selenium</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI68840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="474" mass="52030" checksum="68D0302A5D61C3E0" modified="2025-10-08" version="1">MSISNSCCAKGPGYASPLEAYRHGPKEKLLYIPCVIPDHSRPDYVATVDVDPESATYKQIIHRLPMPNLNDELHHSGWNSCSSCYADPTAASRRLLILPCLGSGRVYGIDTATDPRAPRVGAVAEPEDIQAATGLSYLHTSHCAPDGSIMVSAMGDAEGSARGSFLLLNQDLSIKGVWSSEDTPYGYDYWYQPRLNVMFSSGWGAPKAFSKGFNPAEVPDHYGNSLYVWDWKARTLKQKIDLGPKGLLPLEVRFLHEPTSPHGFVGAALSSTIIHFTRPADDESWVANVVVEQPWVKVEGWVLPELPPCITDILISLDDRFLYVSNWLRGDIVQYDISDPAAPRLAGRVWLGGLVRKGGPLKVLGGLPEDTPEPPEIPRVAGVELRGGPQMLQLSLDGKRLYVTNSLYSPWDKQFYPSLVEGGSHLLKIDVDTENGGLKLDTDWIVDFGKEPDGPVLAHEIRYPGGDCSSDIWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH80</accession>
    <name>A0ABQ5SH80_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013315</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07833">
        <property type="entry name" value="STKc_CDKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1400"/>
            <end position="1700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="373"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="482"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="618"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="855"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="937"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1126"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1211"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1714"/>
            <end position="1751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="642"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="707"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="894"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1168"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1211"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1719"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="1431"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1751" mass="183470" checksum="129C815B223ABF29" modified="2025-10-08" version="1" fragment="single">MLRAAHYKADSVDGPGAGKSPPKHRSGGILDLALTAADKYALSLLPTALKGTGLASNDGPATDYRAATQHSTNGISAAFKMRSPAALHNSAALPQEAQLQPGVAGVATSWDASLKEILAALEPETRTLKPAKLRDHKYPNQNHGMPTNTVVHSPRRELDEALITLPGGTRRNKSSVPTYTNAYTKGLSRQSRRHEAQAPVHSGAPQSMDVPYDLVPSVKLAWAGPGSGTYSTTGYGGSPKKYTSQLVGRVSPHYAGTPGLQQSMSTPAGNQDPLHLPEIANGNHHSVVNQQSSFKTSSHVLPSFQSWSHAATPSTAGQSRRALPYVSTNTPSLAKPLTVQYHPVLKSPSMGAGAGLQNAQPAMTPVTAVGSRGTLQRPATDHGSSSQHAAGAVGSGEQTRPYPRTAYGGQPHRLPHTPPEVAAEVARLRHAQELNGVINGVRSDLLQAAAPRFDAPVKSKFLQMYEDPNRPLAKLNPKQFLGDVAPTSQHNRRHPPPAASADKPQVSADGLPLSSTPSSTRSFMYERQLYPLSSDAQIGDDGIACDSADTVASSSDGPDADTILHLQPSELPDREMPDEGIQSEQPGMRLEAETVSTSRSTPRSLVVGREDTVLSVGELGALPDNGGSLGMAADDEDGCSEGAQAADQPGPAGGDFAALEVSGRGEAVPSDGATPLEAHSRRLSGPTLEAEPEQDPARTVLEGRGSGQQPAGRSGPQQHRSSVIRGTAPATEPYPEGQAQFLEDGTGPQRDDQLHRSGSAPDVVEDCEEVTSAAPESTSGLDQPESAQGAVVTEAAPAGEAVDQDSKKGETVALDHAGSAGRQSPAHHMEGELCDAAPHVANQPTASFEEGGAVSVNEPIPAGAPNAADGPLVHDKGALNTWDGGRTGDLVEPSGAAAEAGQESAASGLAEMNALGAALGAPTTGIVDFAGNHVAAEPPEPVMSGQPLPTKADSAATPSVGRPSYNLPPGVTPEMARASAICIQSHARGYHARRRMVTMRKEREALLAAQAEAPLSPYPPLHKQIAYELPPGVTPEMARAAVVRIQSHARGYFARRHVAELRCGREAGEAALVTAVTAVAMRKLEGYELKAGITVEMAEAAAIRVQAHMRGHLARRRVKHMRAEISPPAEPAGALARGLTSETEDVTDGERAECSIAGDGQGQAGQKSAEEDATREYDATVPTGLVSEKTGPPPALSEPEEFTIDALQAALEEEEDEEEQQQQDADATSLRPEDGAGQEPSLLDMADPASVAAAAAAAAAAKGAIRLLDDGEYGLDGLTDTIAAADQAEEASMARLAANSARTSAMFQGAGAEMRFSDDEASALELMDNLDGLLQGDDEAAAAAAAVAAATAPADGELVSISADGVSEPQALGVNTSGGPAPNPMDLTEPGGVRVLAGKYEVQHVVGEGAYGMVMKCKVRGTEPVQWVAIKEFKIDDNDPDAEDVKRTSLREVAVLEALQHPHVVGFVDKFMVGDRLFIVMEFLPCNLLELLEAQPGGMDRDAVRLIMFQLCSAIAFIHSKGMVYRDIKPENLLVDEHGTVKLCDFGFARYLPSGPSSHLTDYVATRWYRAPELLLGPPYRDALGKHVQYMYGPPVDMWAVGCLMGELLDGEPLFAGDSDLDQLYRVQQILGPMAPGHQYMFYANPHNTGIVFNIKQPMTLAARYRGKVSEVELSFMSGLLEMDPDKRLTGEQCLQHPYLVDLAAAASALPAPSLTASDTPTGNNTEDGGSACTTARDGKTPTLSAAGGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHT7</accession>
    <name>A0ABQ5SHT7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037736">
        <property type="entry name" value="PSA3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36770">
        <property type="entry name" value="PHOTOSYSTEM I ASSEMBLY FACTOR PSA3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36770:SF1">
        <property type="entry name" value="PHOTOSYSTEM I ASSEMBLY FACTOR PSA3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="233" mass="25497" checksum="2C203B8572C76EB5" modified="2025-10-08" version="1">MLLHRLKVNLNGSNSIERRPCAGRMTVMARAIQAKEDGFFSSIKNVAKKVQGALPIVGLVSRLAAPEGGFDELAYPEFCRFIINNAPVSYRVAQTELEKAYGKPANSRWVLLVLWMSKMGVGLVPTKDIIAASKRLRVTQDIEIEVDRFDSAKGAVLKKYDMMQRPEGKLEDKVNVAVDALCTLCLGLKEGEAVPEIAAPLLRDIVKAAFSDASDELVATSLSNRSARAQAYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHW8</accession>
    <name>A0ABQ5SHW8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">VaNZ11_014107</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Plastid</location>
            <location evidence="15">Chloroplast outer membrane</location>
            <topology evidence="15">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF135">
        <property type="entry name" value="TRANSLOCASE OF CHLOROPLAST 120, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AIG1-type G" evidence="17">
        <location>
            <begin position="403"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1060"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="163"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="278"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1060"/>
            <end position="1078"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GLI69475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1091" mass="115529" checksum="8B257A73CFF3C1EA" modified="2025-10-08" version="1">MQQSAMSTRSDDPRDNGDDVEENSEEFEEQAEEDEYEEVENEALAQGEVKNASAAAAPSALAAAAATAPTAMPTLPERPKGGLSSLPPRGLPSLPVKSGNLQLGPTSAGTPPPQAQAQAAPQASGSVQEAAAASTPSQPVAAMPKLPPRPGMGAPSGLGLGMSLGPRPAAGAPKPVAGAASAEAGASASASAAPAAASTSTATGAAAIVEPAAKQSAAPSGLGLGLKGPGLRPTLPPRPGATTGASGAAPGAGAQPKPPAQPPASSKAPTEAAAGPRPTLPPRPATLPARPPGEVVAPAAAGSVASAAAASQDVVDSTEDRRVQRVQRAAHEIRVRLIRAATRLGLSSRSDQVAQFLQAIERSERMVGAQHYKGSRRVDLIAAAEREARALDASEPPHSLISGLRIKIMVLGMTGTGKSELINSLLDRPAAARTSPFREATRRVRVVRGAHNGIPITFIDTPGLHAAAARTAENKAILRSVRAAYRWHKPDYVFYVDRLDATRPGLGELGLLSLITESLGPGVWKNTMAVLTHAHAARVAFGAQYDVNSRQRRNIITQLLRQAASDQQTRNPVFLADCHPSCPTNSLGQQVIQEGPSAVPWKQQLLSQSLGYKSYNSATTLFKELAKAKAGKTAAAGGARGPQDLFKQLMRSRLPPLTFFVEQMTEGVLKPEGWATMESVAGLGEEITEDEEAENFGHVYYQHMYDMAMSGDPWAQREYAAMLRSYDKAQETFKASYEEADLDHAVEYGIESYVVDPIDFGPSFDPEDLFSYRHAYAEATDSAFTVIPSQDYYGPEHEDPLNGLVLQYESQPFARHGWGGLPFDLTVCLEKDKSSLCLQGETHVSVIHSVPPFGPHQVTQLTGSWEVLRPNIKDVMYQLEIDTFKDGLLGRSDHAGCGMMFARLAEGGNPRKGPMAVGVRLQDTFRLGAFKVEACASKVAVQGPTGGKEEGWGARAFVSYDWLPGVGIALDAIQERGSAESGKRQRGYGINVTYDWEMLGAAFGAEFDYVAASKSLFVSVNAFSGNDYRLGWILLLPAFNYAKETLAGLWTRLRGVGRAEEEEEEAEGQEGEGEEEEQAALAAMMSQAADM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI21</accession>
    <name>A0ABQ5SI21_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="160" mass="16914" checksum="3E63F08C9BF3C734" modified="2025-10-08" version="1" fragment="single">MDPGSADDELVDCLVGASGITKRQFQAFTTLVDEVYETYGISGEPRSGSSRRRVPFNHQPSMQESLLQPPLLPSPRSPFGGCPRSPHPAGAANHTARYRWLEADISPKGGDGGECSASPTRGSGFRAAGCSTRRCPPAASALHHTSSTLQLHASRTPLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIW3</accession>
    <name>A0ABQ5SIW3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="356" mass="35967" checksum="7AE06CC0BF2BDA96" modified="2025-10-08" version="1">MSGLFNPFAEQEDQDRGASLYSDPLAAYASAPRPPPGSSAAGPSQGPPSGSGRGSTSPGGRFSGRGASAPHSQTPLHQTPVYGRGGGGRRWAQQYDPSAHRYGYSGWEQQVPGPLQRPVPPAYGGGYAGPGSRGGYGASPAVPRGGWRGSCSGGMRPNKWVRTEPLDAAGSPAPVPQQAAVATTPRPGLGQPQAVVGGPGGRGGLGRSGFRGGRGGRGGDGGWEKGGEVTPAGIAAYYKPTFSADPWAKLIPRPLQPSGINAVNIGGRSAPGQHSIDTRKLGGTSLAEIMAIDLPGDEAELHVAAVAAEGADPSIAISSEEPPEGGEAEQGMEPSPEEGAEGGRLGEDGAQDRPWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIZ0</accession>
    <name>A0ABQ5SIZ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Carbonic anhydrase</fullName>
            <ecNumber evidence="4 9">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_014410</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="4 9"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03124">
        <property type="entry name" value="alpha_CA_prokaryotic_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041891">
        <property type="entry name" value="Alpha_CA_prokaryot-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF265">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="10">
        <location>
            <begin position="82"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI69734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="319" mass="34258" checksum="1C19EB404A10F04B" modified="2025-10-08" version="1">MRSVLDKSASALQRSQARRALCKVRASDSTGVDAQPSTSGAASSTGRLLIDRRQLLTGAAASVVTFVGCPCSICKPGEAKAAGWSYGEVAGPPTWKGVCASGKRQSPINIPVNTSAPKVEAEMGDFDFAYGSFEKCDVLNTGHGTMQVNFPPGNLAFVGDMEIELLQFHFHAPSEHAMDGRRYAMEAHLVHKNKSTGNLAVLGIMLEPGGLIKNPALQNALEVAPETPLAKKSSPKGINPVMLLPKKNKAGARPFVHYAGSLTTPPCSEGVDWFVFMQPIKVPDSQILDFMRFVGDNKTYATNTRPLQLLNSRVVEYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ92</accession>
    <name>A0ABQ5SJ92_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046811030" description="Apple domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="59274" checksum="C970720FC527CB9F" modified="2025-10-08" version="1">MKFIILLGLFLLGANLAAAGPPPPLSCASGWDIGGIQLDLLPAISAVSCASTCLTTPQCTYVVHFNDGTCALRKAPWTRPSQPSSAVTQACSVSGAPLGLPRAEPEVFQPGNLPTDSEVFALCAPRFSIYGTASDTTFESELAECVGRCKLVGTPDCIAVEYNHDDGRCRLLRSTYEGPEEEEEEDDEAGATGISVLHTVCSLGSSRAVLQLHGETKITVNGLSGSHRTRADRTLATIASPPPRKSPPSPRPLPPPPRPRPPSPPPLPPSPPPRPRPPSPPPSPPPSPPPSEPASPPPPSNPDAPSPPVNGWPNTPIALPLPDYPPGAPSGPGAPPDPFNPCPVPGRPATLTTYRCAVGWEITGTVLLPQPTGVYTAAQCEALCVANAKCQFYVSYLDGKCSLRADPWIADQYRSSPSSIVNITCSKPGATQPLRFPQSYQTCFCQWSYCYGIANCVEGFEAYGQWGFFSSQNPNGFVDMGMCMVGCQANPHLCSYALWYQNGTDILCLARQVPFVYPTPLPDYYNVQGTTGPSKRANIMCEVLNLGTMRLDAGRPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJF8</accession>
    <name>A0ABQ5SJF8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014928</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07751">
        <property type="entry name" value="PolyPPase_VTC4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14447">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="625"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="669"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="711"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="497"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="574"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="758" mass="84654" checksum="233028BDF6741572" modified="2025-10-08" version="1">MKYGKYIESKIKGEWVDYYIDYKGLKDLIKKCQKEAETAGEASFSPRTTSLTVQRYSNTKDSSQELFFRRLERDVDKVNKFTNKLVEEMRASLRNLNSKAEKETAPERKDELLQEAQRIGDDFLALEKFVNINYLGFHKILKKHDKCLPHAPCRQFYVAHLHQQPWVQGNYSDLLVSLSNIYSKLRGDSSGEKNEDAAQGFVRSTTKYWVRNEDVSTVKHHVLQHLPVFQFDKNNFTGDAQLINSVYMDNSNLELYHGRLDKKPGAIALRIRWYGSNPPTMVFFERKTHKESWKGEESVKERFTLPCDKVVAFMDGEYTLEHALADQEAAAARKGKTLTEEEKESYKSLFMEVYKAVDSKQLKPLVRTQYMRTAFQIPFDATVRISLDTNLCMIKENPEDGPSCAATGRWYRDPALPVMRTEITRFPHAVLEVKLSLQEGQHAPAWVQELLDSGYLTEVHKFSKFIHGSATLFPGDVRAVPYWVDDESVRASMLASAPSPLEGNTPTPESAPGTSAPPPAAAPGATRTKPRTRRAGATPELEELSHPLLGEQPTLKLMPDPNTVQGFRMQGLSAGSGGRGGGQRPQPGFFSRLFGLVPPVPAGAALRTTPMRIEPKTFFANERTFLAWLHMAVTLGSVSAALLGFAAGADTDATPESGGEAISRHLVELIALILLPLAVSMCTYALYVFIWRATNIARKRAVHFDDRVGPLCLCGAVVVSLVAITLLSILDFFELLASVDSKLPPPPLPLALSMSMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJP3</accession>
    <name>A0ABQ5SJP3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pseudouridine synthase I TruA alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014017</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF9">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="5">
        <location>
            <begin position="597"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="486"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="493"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="542"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="828" mass="89895" checksum="9E409E399E86B3A0" modified="2025-10-08" version="1">MLHRTAGPLSAAPRAHRVAASNLPVIAGFIYQTSFCHLASSLVIRSPSISTRYRYSRSPFPQLNGIHDSRPGRLRTLRNVTHWRVTLAISAPSPHRILLLSKGCQRGFSAAAAESAMVTSKAEPETAACSQATASRILAVSVSSGTDTSDAVPVDASPQPSQSAISPSGREAAKAAAEAAYAASGGALRTKRPPGMVKRSIAMHLGYVGTAFKGLQVNRLGAPDDTVEEVLERAVFGAGLIVESNFGDLKKIKWTRNSRTDKGVHSVATVVGLRVLLPGDERFDTDPEGLEIASTLNARLPPQVRVFSAQRVQKKFNSRRFCFDRTYEYFLPAYLLLGPHGGAAPVAAGNVDAASTAAEVDTETEKMQEGHHLHHQHHHQHLQGCMRPQQLQWDEDLEAARAALVKLREALSCYVGTHPFHNYTAKRKTYVDTAKDREARKAAREAVIASAGDVVDCADGTAGCTPVAVEVDTDEDEVRLAAEAAAETAVAAPDDDAGAPGDELDMDRRYTARKPVDDDDDARNFPPKQQRPRPQATKKEDEPLEEDDEEGETDGAESVDGEDEGTDVGGTSPRRRWVQSCRWLYEKSEDKRDRVTVRHFRTITSFTADDPAPLVPGGVPCVRLRVRGMSFMLHQIRHMIGGALAVARGSLPLQLLRASLAGHARVTVPRAPPHTLILADCTFPPFRKAGADEARVHRWSGERLQLRTGGQSNLTDFRRQSLDLALNELLHHPDWERFEMCLPKFYWDPQAQEEVIRAYATWEATREERAKQREAAAAEAAALAAAATAADRLGPEAAANVPEAEVQDLSVAPATAEVMPTAAAPDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKE2</accession>
    <name>A0ABQ5SKE2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_014711</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21742">
        <property type="entry name" value="MobB_NDR_LATS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05599">
        <property type="entry name" value="STKc_NDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059233">
        <property type="entry name" value="MobB_NdrA/B/Cbk1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="117"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="413"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="522"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI69965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="543" mass="61564" checksum="2B9A63CD88C2E30A" modified="2025-10-08" version="1">MSNTGGLFTGLQRPFSARRKKTEDMGKVDAGGSGLSEQVVSESTRQRVEAAKQYIENMYKVQHQNIAERYARRSALEKELQREGLPESEKQLIISELEKRESDYTRLQRQRMTADDFESLRIIGRGAFGEVRIVREKLTSKIMAMKKLKKAEMLSRGQVAHVKAERDVLAEVQNPYIVKLYYSFQDEDFLYLVMEYLAGGDVMTLLMRKDIFTEEETRFYIAETVLGLESIHKAGYIHRDIKPDNLLLTRTGHVKLSDFGLCKPVDVQTLPTLTEGEEYNDVGMMPSTSARPQAEQLAHWQKNRRQLAFSTVGTPDYIAPEVLMKKGYGMECDWWSVGAIMFEMLVGYPPFYSDDPLTTCRKIVNWRMYLAFPVEARLSPAARDLICRLMCDVDDRIGTRGGVEEIKSHPFFSGIDWANLHTKPAPYVPRVEHELDTQNFEHFDEDRSGNQGAAAVNGAGRGNKWLAKADPNFIGYTYKNWEAVTPMREDAVQLKKKAVTRPSLAQLQTNLQAMSLSGPQAAVSVSAGPSGQHQHSPMQRILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL47</accession>
    <name>A0ABQ5SL47_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tubulin tyrosine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014548</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF147">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="413"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="562"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="777"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="838"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="881"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="954"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1014"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1140"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1228"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1341"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1464"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="441"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="566"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="579"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="592"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="787"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="806"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="881"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="958"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1024"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1044"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1065"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1341"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1389"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1492"/>
            <end position="1503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1630" mass="166207" checksum="1D5D956B4FECDFCD" modified="2025-10-08" version="1">MAEVSEGEAEVEGEELEGEDSGQPIHYLVPVTQEPPPESDNKKKVKGKRKKAPPPIRINLSSCKYEVLRIVQRKLGWKEVGDDDDWEIYWTDTSVSIERIMKLTKIQKINHFSGMLEICRKRAMARNLMKMARQFPQHFDFFPRTFILPGDKEDFLADVRTRGKKQVYILKPDAGCQGRGIRLVQGGKEEVVTRVLGEMATPNVVAQHYLSSPLLIHGYKFDLRIYALVLSCDPLRVFLFHEGLARICTEKYSPPKASNLDVSFMHLTNYAVNKKNEAFVAAVAANGDNGEDASKWCLAQLQAYLTSLGHDYNKVWGDIGDLIIKSLCSVQPILRNNYRSVLPPDNDGFSCFEVLGYDIMLDDQLRPWLIEVNHSPSFNIDSPLDLAIKEELITQTIKLVRIDPKLIAKTKKAEKRAATSRLLEPMHIKKPPPGRQTGPAAAGGGAAAGTPGGAGTAGAGGGAAVGNSSSQADGKGTEVEGEEGTNGNGSAGAASSMASFLKPRTAEEYETWRAKVLAIRERYEAKHLGNFTRIFPSPDPAKQALYEELLKGANEIFQASFQARTKRPDAQDDKKKKSPSPPSLAPPPPPRRPQEDLETESQRAERLKAKAHRDNLAQKAVERRKFKLLEQQRAQGMPVPGYLASPQPYLLGGVPGSANGGSMSDAQQQLNPGHPLYYMLGPYQYDPVVFQQYYQQAYLYGSAGAVQRMSEVFGERSDGVVSASSSRPVSALSSGSAVAVAAAAAVAAAAAAVDVATAAVQPPPPIRIYSGPLARTAAAGAGGGQHPISSTGTSGRNSPMSGSAGQQQQQQQQPQQLSQQSQPTHGQLIQQQTGALPLQPFLGGQRRPSSATSRASGGVGIGCEAGEWGEQPVAVAVGGAACSGRSGGSRTDSPSSALPGPPGSGPVPTARGSGSITNGITANGHTFSYGFNAPFQHHQELHQVATTTTGLAAAATPGGPGVGGGGGGGHAVSQPQPIRPVSVGSAHNHVRHAHITNAETTSAYYSGFSGFQDLGASDREQHGPGPGSGQVAGPGPGRGLIINGSRPPSASAGPPASLRDKLQPGSAVGSSAAAVGGTTGGGTAAACSGLSRKQSFAAALNASSIAAAAAWDAAMAATAAAAGQDPATIMQYLGCRPRAESAGQRLRPTSAAGAQRSPSPLGALPPGNPACAAAALSGIGGGGGSPSISRNGSGRYASADMFALGMTTHTFMQHSQPTPPLVVVAGVPGSANPGSPKAGAHHGNSNLAPPPSPRLFTGTPGAVIGTPPGSAGSVNVGGFGASPGLAVGPWDNGTTANGLGLAVGPATAVTGSSASTAAGRAYKQSRASGVAPYTMLMGRNGAAQPPASGAASSAPAGSQTLGSNVNPPKRRSDSGVQEEEDAPAPFIYQTLSIPDAATSNQPERYGGNVAGRHWSAAPPSLSPMAAGVSAAAASGSSRQRSFTGAGSYVPVVVVTGATAGPAGTGGGAAGGAPSPSGGSLTVRPPMYPPAGGSPGLRGAGGPSSGRASGVGASSGLGKVVGGGTAVAGSGSGIVTASGRPSAALPPTNLAPSLPGAIPISYLNLSMTVRGGGGSGAGGGSSTSIAALQSGIEGLQITATGLDLDSQLRSAALAALNRKGLTIKGRKVAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLW4</accession>
    <name>A0ABQ5SLW4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046458924" evidence="2">
        <location>
            <begin position="20"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="185" mass="20537" checksum="A9ADAE43E39DB47F" modified="2025-10-08" version="1">MKQVVIAVLLAVIVGTASATDPDQNSWNFMIRRNSKCNGTYETLIESKYYSTNVTCKTNVKLALETYNKAACPDTKQGSLVWTCMSGYDFTTGQTNKALINDWTAFLSGCEVLYIADRKNTAESESTFVWLNNSCFGRYSNMDQFVSYLRGTGSSGGNAEKNVISSFHVLIAVIVAMFLTYWQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM70</accession>
    <name>A0ABQ5SM70_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046024405" evidence="2">
        <location>
            <begin position="23"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="250" mass="26037" checksum="10E54262CB12A724" modified="2025-10-08" version="1">MKHLSHLFLVLLQAGVVAVVLAAKDSTASDENTSTDELKFQLVHEPNQVAHVRNGAKGPGTSTLQSLVAPPPPPPPPPPPPRPSNAISYFWGTIILCYTRPSPPPSVPDVPSAPAPPSGFSDGKIGPMDSNTAKDASTLINFSDPNHTASLTPSVPSPPPPPPPPPPPPPPPPPSSPPSPPPPAPPPPSTKPPFSGRKIRPPSSPRAPKPHSATRDPPGPLPPKPSPSLLRTPTSLRPSVPKKIARRMLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMI2</accession>
    <name>A0ABQ5SMI2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016103</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF69">
        <property type="entry name" value="FERRIC REDUCTION OXIDASE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="792"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="835"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="4">
        <location>
            <begin position="477"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="271"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="706"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="712"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1016" mass="108192" checksum="FEC573112275F6AA" modified="2025-10-08" version="1">MATPSRSSAAAMHRFALRAAHATLLIIIGAGAITFAFFYTITPTRWYNVRATELVTWRNTLILQYKFANATTGKTTPLLSGGLSFILIWQWCGTLAVALGTAGMLWLSSSSATGTANATANPPRTASPPKMYPERQNNSSCARSCWSFPAARAAAIKQAIMRVRRLLRYQVPPRSLWRRLLGPGGLTLLDWLLVLLWFGLHLMWMREMTMRVLDSRSTTRPPAKGTAVVVRSPPLIKAPSSSAVTNRSSNSFIAPPPKFMLPSPSVASINASSNGTANGGAVRRSLQTTPSAPAAAGAPAGGAGNTVTGSPGGNFSSSPSAAKTPSSRPPPAKAVAPPPPAKKLKPLPAVVQDRVSNSLRPLSMCQPLRTYWHLVSPSTQVGLADWTSCCSSFPFPDATSSTGFWDPISPPSSSTTGGLESAPCWYTPCMELPTWASGRATACVFLYLLDVVLRTIQQNFNCVRLVGRTAADEHCNNQFCRNEGAAIARVSSEAVLLSLSIPCHQSLRWVGADMVFLNVPAISWWQWHPFTITSAPGEGPGSENQMEIRIKSYNRWTKALISQVSSNPRFSLTLYVSGPYDSGNRKWITDFDTHVFVADDIGITPVLGMLAELLAFRRRQFAAAAAAATANDANADNRSVGMRRAILIWVSRTMDELRALPEDILQEASMKEPWLDLRLYLSAPSVASAVKVPDIITTDKAYGENKGVQLQPSGVSRSNSGSSSPSPKADPIASPASVVSSPSDGVVFAGSSPPVAATATASAVTTAASLGLEALHGVASRPLSHPYMFNPLIWMVAVLLGFGGGFAGLICAQAYDAHKSRTVALRQDFSYMGMLQFSALGLGATLPPAVLMLGLHLYRRLARSSSWPRSGSGSKTSISLFERGSFNCKEVPAMVPFGNGPSTTGTQLLPAPTLTSYIAEGRPNLAALLHQIAITGSTCSTCNISASLSRSESNSSSQSGEDGCKEAGVEAARVAVYAGGPKPLVGSVEETCASHNGLWGRKDRAYLHFTFITHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNE0</accession>
    <name>A0ABQ5SNE0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016749</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1174"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1978"/>
            <end position="2010"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="2051"/>
            <end position="2083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="231"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="453"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="530"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="735"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="799"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="917"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2842"/>
            <end position="2886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="246"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="314"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="535"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="752"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="802"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="821"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="965"/>
            <end position="981"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2899" mass="293182" checksum="B1D6A0FD28A8D9FB" modified="2025-10-08" version="1">MNTGQGKSAGVASKPMAIKNNTVKPNGDGSPAKSLASASSKPAAKAAPASPPKKPAGDGTTAKQAAVKPSSTSAPVSPKNPISSKTPKSTAASTSGAEPQPCSPPAVAAGGEKTVESDGNVPVTSKPAKRAPVLARATADPSTAPAAAKPAVPETPTAPGDPVITAVNPKPATAAVECPDVKATSSGSGSSSAAPADNHVTKDPRVVIPAGDAAAAAAIAADGSEAMLSKRPSLVAGPAPVPPPAADTATAAAGPRSQAAGPPLRSGSSSAAASAVAPEPAPRPTSTITTSSSDVAADSPNTSTAEHEPAAPQTTTTTAIITTTAERSASGATTTAALAAAAAAAASVPQAASEPMTTSAPVPSASALDAASTADPVLNRPLPIPVEKAASVRAISPVSQIASEPAIDCITVKPAATQPPVKGAPSLKVISAPPPLRTASMGAVLAFDGTADAENSAGTEPADTAATKPGASKAPALPPRSRTGSTAWEAPPIAAGADDVAAVPAASAPVVLFEASVSTRSLLPQDLEREAEAVAATSPQQPSTTTTPQSMPAPSVETATAGAAALAAASSAPAGCVVITLEHNPSGPCPVELSWPALDPAGSGEGVAWTCTADLMTAAMKALRAKYGAVQASPAVNAFFVEPAVGSGAAGALERRRWLSTFDALPQELLAAASSGGRANVILQGITCGEAVEPQAAGAATRTPAVSTMFPLPGFAESVESVGVGAPPSRVTFLSEAGSSRASPNPPTRGLLRGGSRGGSNGGGTAAAGPAGGRILGAGHRSVRIRLEENTVETLCVESACPSPSSLRSQVHGTRLPPLRNDSDRSRADDETTGPSPFRGRLGVTRSSRRLIVPACDLIAESVASSECDNSVRSPMAMDATAAAVVKRDPVQVLKQTNSLGAEFEANVVTTAAVTTTARDARGGEEGENGGGEIGAGAAAGTRSSPGRAAASSVVTAKRRSSSTGMEGAPPAAAGAVASSMGRRSSLVVRSEADTGSGEDLEAAFFPAPAAEWIDLMGAETTDSQAPSQPGGCLSCFSTGSKAAQQQQWRERLQEALKARDEAEVAAWVTFHCGRSGTRNQRELDAMLWLAVEARAPGLVKVLIRGGANPDAPRPMGLMADAAPTRGSLGSPSVARPMPSARVKDSAVVSAPLPPPPAMAWWPGSAATATAALEGLTPLMRAAELASYDVVEVLLAGGANAIVVHPTTGWTAVHRAVLATSPSTTAWGGRRLFPAMAVGVGSNSSSGSAVTAAAGKVGVRGVTSAAAEKGGGGGGGDDAAASALHCLRLLVQRIGLQHAAVLTTAGRYSDDLVRFAVRHGRSEALSWLLSTGLSPDGGASSADDDDDEASCAFLAGRLGLTVEVWDTPPPPRQGALRDRARPLSWLISPEERLFTERRRQLLMGAAAGGRVDVLAAVLDKPPPLDRLRRDELEAAIGACRLAQPRNQRQVEELLTRKAHTYMSWERETLPALRRFDEPVIREWLVCDRPMPVEVDRALAALLSSERLGAPLWHDNAAAAVQVLLDGGADIEHEWDVDTAQLPHLMSGREIKTLRPLQAALLAPAPAIARQLLAAGADPRRRDAKGENALFFSIRTAVAASIIAHECNDPDVIRMVRQQPSGISCANSSGRSGAAAAAASEVTSSECIDVLLATEHAAELLAARNDDGLTPAVLAALADTKGILEKLCAAGADPWATPVAAAPGAPPVPSELVSSGNRRSASENSSLWSVGHEAARLGALSTLELLLGGPSSTVGRKAPLTAANAEQAAVRRRDALVGAIRGGRVGAAKLALDAGGFSGSLYEAELAAALALCKASAVQKLPAAADIAAATGGPHGPDAPRYEALAAFLASRAHVYMNWEGEVLPAIRSGSVSVVRRWIAYDKISAATATRALCALVDAAQQCTSSAASVETTAGAAAASTAAATTALDGDAVAAGVLSRLYSVGASCLTSPGKAVADVAAELVKAGADVNFQQEPSAGGQSLLMQVAVIGHLPLLEALLALGARVDAEDAAGMTAVDHAVLARARLADNGCLAALLAAYGARRINAHRNHAGDSALMLAVRAGDSTAVQLLMASGATPAAADASAPARTGSMSGAVAASAAPAAGSISASDVGAVSRTHRISPSASDDPPGVSTAALVTTASAAAAIGSDARPSSDVSTTAPSELAALNGRSLDTFTSGLTRRGSALYLAARMDDKMMLQQLLRKASCNDDDLASCRQEVQLGALHGGRVALLEASLAVPELLKSVPGPAEGGGATALGGAATVCARLSEQAAVRLAEAQGLVVELQAVAADASAAAGDAGAAGAVPISVADVTRLQRYLTAKASALEALKGAHSRMGEFYALAKLHMEALEGALEAKKELVSNSIRDSSDAQVKLMAATTRLTASKDSVLRSVLARRDAQLGRVEGLLEKKLAGLAAAQRRAEAKVTSDVEGIIMDMRKLVGRKLKDIDAKKLQAFGHLGALERKLDVVWGRRQRLLAGRPPAVRVALNQKMGALMQQLGTRREALNEAVSAAIAKMEGGQRRGTALVNMEALRAALAREQQDMARGACEAAAIAKAAIVQRAEELLRIDKHDQLLRIVGHMQAEVTRGSSSTAAASAKALAALQQAYAVAVGDLERRVAQARARMQEHLTELMASHVDLVKTLTLADDASQAEAATEALAALPDAQLNPSGTPPDVAMLLLKFEGAAALDGVQHAGLNGGSGGENGTSAQESTLSVLQRVATVEETIEDMIQDMAAEVLHAFPADGICDTLAPLDLAPLLEPDSEISTAGLGIDRLRQDIAAIDFGLPNLDLEVGDLDLSALEDDVGGQGGMRAFGVGAAAMGMGLGAYDNYDEDDVGDDVGAAPQGRYGNGGGVGGVIRNRRSSDEGDAEPGGGRGRYHAADYGDRHEDDFALSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNR0</accession>
    <name>A0ABQ5SNR0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="531" mass="59345" checksum="30952505DDCC294C" modified="2025-10-08" version="1">MATALGTQVIVALAFGAIGLIFSVLSNVFQILNNVRLNKYQLELEAWQRQQTRFENVEELMEVYKKPLVESANELQLRLSLLLLSRQKIMDNGERLQAYMLHSTLYLFGAFLGWLEVVRQEIVFVTGPRAEILNEVIDAIKFQFTGETPVQGVPCTAEGLEYSLESPWHILQLYHVELRAIGEVMLSERTYDNDKKDIKSTNLAVIGYAEFVRRFTMREPTLEEFTANGCDYANFREAKDHWDASKEKLLQENLRPLTLSVRKWMELQPESRPTERIAMLQVLLCKLIDFLDDGVECSNSDSSSGARGGRRGGRPPVPERFLSRNEWLSPKLDHLRLDALMWLWDRPFMMDCVDLVDPVRRRSWLSLISAPNSWMNVFGEEVPLGVFRKLAFPGFREDLQRRCVGAGGGRRNTVGGSPYLAAAFPPKAPTVSFMSDPKYYLQIWAPYLWAMLTCKALHDGGPLQDPFRCSPYVARGPGGATGGGGGCSGGGGAAPAAAAAVPYPRLSAVPPLSPPPAAHPRPLYINPVANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNS3</accession>
    <name>A0ABQ5SNS3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADP-ribosylation factor-like protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_016939</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="21525" checksum="8475FF9C5FDA4DFB" modified="2025-10-08" version="1">MGFFDKLLSFLGVSGKKVNVLVVGLDNSGKTTIIERLKPRPKQSAEVAPTVGFSVDELQKGSLTFTVFDMSGAGRYRTLWEQYYREADAVIFVVDSADKLRMVVARDEMEHMLAHPNLRKVPVVFFANKKDLPVAMPPVEIAQAAVTALPPCLGRWALGLDNIKDRPWQIVPSNGLSGEGIDKGVDWLAERLGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPT1</accession>
    <name>A0ABQ5SPT1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_016943</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF28">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00607">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047125608" description="SRCR domain-containing protein" evidence="6">
        <location>
            <begin position="37"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="7">
        <location>
            <begin position="457"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="7">
        <location>
            <begin position="767"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="895"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="956"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="1017"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="1078"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="1139"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="1200"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="565"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="570"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI71664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1301" mass="139119" checksum="197A02D56209D180" modified="2025-10-08" version="1">MMTTAEQARAPERGRPFHTNVLLLLLFLLRPDAVHGQLLSDGDDRLFVLMNNAASLTKNVAVSSSSKQSRLDNLPSPPEPSSPPRPPIPANWVNLALGQPVYASSSFLSTICPNLDCGPKYVVDGNTTSAGVVYQSDFGDVSPWVSIDLGAPAAVHHVVLHARNGFLIPQQSGLEVRVGNTSITNTEDTSLLSRNPIVWRQSGATTDGAVMDIYLDSPVVGRWVTTQSSIPFMALVEVQVFGVSEEDPLAPKPSSAPSPPAAAPANISDPPVSGIAAQCGYAGRISHMNDNGICVDVTFSCPYIWAYGNVLVNYRFIYQKADGSRSSGTTALKMTCMDRMYAVPQLYVEFASPFSLGPGDYLVLQQKNFTASCKSKPWWKSWEVQWEDVLLIAFTTPRTFMYLFDGIPLGPSVNWTSQNCSLAPPPPWDYSSYYNYYYSTPPSSDPSDNGNNQPYDLRLTGGSWAGEGVLEVFDGLNWGTICAEQFTDLEAAIACRDLGYPTGAVVTPNPWDSVTSSIILDRVDCRFRINNSVGLPSETFSQCFRGVLGMPYCTSNKRVAIRCYGNDGPTQPPLPPSPPPPRPPTPHSWINLALNKPAYASSALRGLDSVDGRCGTICGPTLVTDGNLARSSQYQSASYDDYQPWLSIDLGAPATVHQIVIHGNLLLQLADRVEIYVGNVSITQTRTFAEAEALLYQNQLVWSQAPLTKPFYSRKLQSFVEPPVVGRWVTVHKVVSLGLVAPTMVVEEVQVLGLPSVPPAPVKRFQLRLVGGRAPNSGRVEMFSGSKWGTICDRTFDNAAATVTCRELGYKSGVAMPGWGAGSGPILMADVRCDPSVHMRLSQCQAYGNESAYACQHRQDVGVMCKDTLVTSESPFTPVSISAGGFHTCATSRDGRVKCFGQNDYGQLGLSDYLTRGDRPEHMGALLPTVKLGPRFTVSAIAGGMFHTCAILQPGGVVKCWGRNTNGQLGLGDSQNRGDDINEMGSDLPTVDLGPGLVATAITAGDYHTCAILQPGGIVKCWGVNYRGELGLGDFEWRGDEPGEMGTALPAVDLGPGLVATAIDAGADHTCVVLQPGGIVKCWGYNLDATLGLGDMIDRGIQPGQMGANLPAVDLGRGFKATAVACGSRNSCAMDQSSGLVKCWGDNFYGQLGQGNILRRGDQPGEMGRLLKPISLGEGYRPTGIVAGDLHICALLQPGGVVKCWGSNRYGATGLEVGQAFGDQPREMGDFLKPVNLGVGLNASAITAGYAHTCALLQPGNIVKCWGVNSRPFAGTLGLGDSNTRGLAKNTMGDKLPPVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ35</accession>
    <name>A0ABQ5SQ35_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055900">
        <property type="entry name" value="DUF7477"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24289">
        <property type="entry name" value="DUF7477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="599" mass="67355" checksum="4406370223F5BE5D" modified="2026-01-28" version="1">MKGEGQTDKDKEGDKAAKEEEASTAPLPDKVVVGGSPEYFVDRKLGKGGFGQVYVGRRVSATKQKDGPQANQVALKFEHRSSKGCNYGPPYEWSVYNSLGGVHGIPKVHYKGRQGDYYIMVMDMLGPSLWDVWNSSGQVMSQEMVACIAVEALSILKELHAKGYVHGDVKPENFLLGQPGTPNEKKLYLVDLGLATRWKDAVCGTHVEYDQRPDVFRGTVRYASVHAHLGRTASRRDDLESLAYTLLFLLKGRLPWQGYQGDNKGYLVCKKKMATSAEMLCRYTPPAFRQFTEAVVNLKFDEEPKYEAYMKLFEPLCGPAPSRPILVEGAAKVGQKRQRDADEQLEDAAPKKKVRLGLPATQWITVYNAHRPMKQRYHYNVANTRVEQHVEKGNDDGLYISSVACCQELWALIMDAGTGFTAQVYSLSQHFLPKEWIMEKWEEGYYITAMAGSMSGSSLVVMSKGTPYTQQSYKVSDSFPFKWINKKWKEGFYVTSMATSHTRWAVVMSRNAGFADQCVELDFQYPSEGIHRRWDAGFRITACAATPDQSAFVLSVPRRRPMDETQETLRTSAFPSSHVKEKWAKNLYIAGIAYGRTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQJ0</accession>
    <name>A0ABQ5SQJ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hflx-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_016922</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="entry name" value="GTPase HflX, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00900">
        <property type="entry name" value="GTPase_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229:SF0">
        <property type="entry name" value="GTP-BINDING PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="GTP-BINDING PROTEIN HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046259890" description="Hflx-type G domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Hflx-type G" evidence="8">
        <location>
            <begin position="313"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="537"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="272"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="545"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI71647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="560" mass="61616" checksum="51449F27CDDF81D2" modified="2025-10-08" version="1" fragment="single">MNCFELGSVLCSLLSINLLVAKGYFIPALGLNVSTHKIHTFCLLGHRHRFLTFSNRSLIQVGKCACRVSEESALQLSFESALLQAQQQAGPRQRTPGRKIEFPPEDERERVFLVGASIKGEQRRYSYDVHESVEELGRLAETAGLRVMGSTYQILDAPNMSTYIGSGKVAEVGRAVAAMDVETVIFDDELSPGQLRNLERQLAATAGGGEGSVRVCDRTALILDIFSQRAQTREGQLQVELAQTEYQLPRLTKMWTHLDRVGGGGQVKGAGEKQIEIDKRLLRDRAAALRRELEAVRTHRRQYRDKRSATPIPVVGICGYTNAGKSTLLNTLTNAGVLAEDQLFATLDPTTRRLRLKGNKEILMSDTVGFIQKLPTELVAAFRATLEEIRDASVILHVVDISHPSAAAQNAAVMAVLEELGVKDIPMVTAWNKIDATNNQEEIRRIAAKRSRTVCISGRTGEGLDELLEMLGEVLERSMEEVRALFPYSSGDLLNELHTGGRVKNVEYTSEGVAVVAVAPPSLVGRLRSAGILVGYDEKEREGEREEEGEGEEEDGLVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQN4</accession>
    <name>A0ABQ5SQN4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_017022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="611"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="643"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="683" mass="72139" checksum="DD91A78F645B1C4A" modified="2025-10-08" version="1">MLISEPKHLGARIRSRNRNLLTAVVRPHTVPSPPSVQAWLCLQRAKLTTVAAQRPPVNRNLLDNVDSTNPDGYSIHVFPPYEGPGGEVQTGAGNWSGGTGPMAQPSEQAPPSHVDAGTTSGDWLCDPQTLHAPSSRFQQQQQQQPVDSCNDNGYAYSFTRQSLNDFPTTSTLHQLPSTVPAVPSARVDERQVPQGMPPTWQSAPPLPGAYGSRATGADWGEVDPLLDWGGVACIETSDSDEQCSSNSSAAPQSPSPSMTDNVSPGPEAGSSSRFYKEGFKAPYNAMASCGGGASATKGPSVDWSLEGPGWPEASPGDRPPFPSWAGTWTSQEGRMGAGPVGSDASGAAGMGGTSPPPDAVRPSDIVLLPGRDASAILPLAPMPAQVDFFQPRTLQERIVQFLGSVGTSVILSKAAVLAGPALLYPIWGPWIRAGLRNLDLYSKSFACVGLWRAQVLDVQINGLAIGGMLISKQPPVVALLVGDPWPGGARVALELPYQFRSEIIRIGDTVEMLVLSRDDSFTSFKVVREVYLPQSGLWLYDYPYVNRDLLVAVSVAVERQRRAEATAAVTEQYYTYDIPAGADIGPAQSPSPGPGPSSGAEMAPGWEFGSGQVTGSGASTVTEATRDNFSPTGMETGLGARQATVSRSWSQEELGNEDPTGAAPPSTSRGYSGPSSWSSGDWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQY7</accession>
    <name>A0ABQ5SQY7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_017202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000125">
        <property type="protein sequence ID" value="GLI71809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="433"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="742"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="898"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1074"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="464"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1079"/>
            <end position="1088"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1140" mass="120581" checksum="97DFD3078D483D88" modified="2025-10-08" version="1">MEPLSTFFRISPALLFQNFTTPRSHASSSFSSPSFADHQTGLKVYCESPASLTSAHQDNPLFEGFPVLPASNRKAEASCSESFAGDEQVTGNSPKLQDQLHHLLTVLQHALEVHGTEGLPDEIAPQLADELEAVAKKLKDKEREVAVERQLRAKVQLALTGAEATIRKLRATPTRGTSASGWGMRWRCNEAAALSPTATTPGPADIWNPGRKSARRTQAAGTTRVGGLRDSVCRQLLQSPSSSMSPLSMTGPSAATSIPIAVVDDASMASPKELPTDQQRSQSCQITVGTTSSCEHVLIPTGIQRMRQISSQSSEAATSSSFHPPLYNWMKAVLSTSTKQAHGRLHSYGSGNEGVGAQRCASTNSSTVQSPAPCGVVGNSIAAAIRFAISKTRTPPLVYGSPLYRRIQRLLAEQRSPSPFRQAVVLVEDAPPHVDSVDQSPTCGGRAGCRMQVAPMPTGSRPVWDQERDTDSASVSDRRSDTTSSSCYTPAITARDDIASLPFRSSVSPFGPTLREKYAARSRNATSDEGSVQRPVVAESAASKTPSMCGGPSLQPAGHAAEAAALVAELGLDLQLDLATADNISNIEVLEHMERWAREQFITKRVLHASKEALLETDGDSDAVGSSKFPIDLSTQALVDTAVQTESVDTRFAVCLQSRVPKPVPFGDGGGGGVGGGGGGNGDGAAAGALGTCQSKHRPTGRGPAGVAHVQCAGVSWDMEGSRDGAIMDDVSADVCALSRNDQSKRSPVLKGKQQTDEAAANNGRPLRDSGTAECSSKLAIWARKRHAAFESVGWKGRRGLFRAIGRRCNPSTDANDGRRVDVDHEDGVPGCHFDHLPRPDITPADSSLSQTGPSCRPDLVRFGHPFSKSTPRPNTAGAVRVTRHGDMAPHVHTLTGQGRLSQRNTPREENTANELPFTPGISAIGGSKGGCHRNRGFPGTSGCGRGHQGDPERVTPLAGRRCTSGVHPLAGVPAGSARNCLPGECGPSTARGLKYPDDTVAFRAEETNGSAAGVPFTPRNTQSAVRRFGDKRSVVPFRLDSAIRFEDEESDVEGDISARTLSSISSVSIIGLDPIDGTNDDAEEDQPDQPSARCLEHKPACRASPRTVKRPGDVKSPLSQYLCGFLASLIPCTHGSDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN83</accession>
    <name>A0ABQ5RN83_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000848</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050145">
        <property type="entry name" value="Centrin_CML-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23050">
        <property type="entry name" value="CALCIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="78"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="114"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="4">
        <location>
            <begin position="171"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="23656" checksum="C3F2D99EF73A9AF8" modified="2025-10-08" version="1" fragment="single">LVSCRGVQVLQGKVQLRSRRREAFAPRLMPRNAALNDGVRGNGADAATSGSGGGAGRVTPADGGGGIGCGGGGGLENVTEQEMLALFKHIDADGSGAITVTELQVAMEMMGVHKSLEDVAEMMDGVDADGSGEMEFNEFKNVLRRAIRARNGMPTFTEWKELGGDLWFRDLNSGCWTGEAALLDSNPVPQEGMLLALETSDLLVLERGDYDHIVENGFDGELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN84</accession>
    <name>A0ABQ5RN84_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000851</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="72"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="249" mass="27082" checksum="31861B5D3D0D9CE8" modified="2025-10-08" version="1">MFCPFPTCFAQPCGRLSQLACPCLTNSHEFVIIKCHKIEHNRGIINLRMSSAPPSLPDIASISRARTQGWTEQHIGYMCQAIAQALEAWACREVPVGCVVVRDGKVVGRGHNLTNKTRNGTRHAEMIAIDQMLAANNGSVQEANFERCDLYVTVEPCIMCAGALSLLGFRQVYFGCGNDRFGGCGSILPINFEGCGSCTRQPIVGSYAGRGFPSKGGLFAEEAVEMLREFYAAGNPCAPRPHRPVPREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNH6</accession>
    <name>A0ABQ5RNH6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF151">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046581300" description="SET domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="45"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="369" mass="41996" checksum="B25D704267939330" modified="2025-10-08" version="1">MHSTFEWRSSPQRMRLAILVFLLCQSVSSQPIDEIDKLFKWVLDLGGEINAEVRHNKDGFRGLFATKAFTENDVIAAIPAATILNTGSFNDSFAVPTLTVLRELKDQNSRFKPYIDMWPKPDRIVNSCNMDLKYAPMWKSTYWEKNVRDWESHLHALLNGDLDADLEYTIREVVGNDPVTLDDLKYACAISSTRYVSSLRRRRLLMAPIFDLANHERNCTSTLSTYEGGNYLYFLAGQDINAGDEICYSYGSLRDDYAVAHYGFLPALEDPPRLSLVDLPGYDPNTPYSHDDAPSEEPFDGTPEELQAEMARLKGIYDEIKSTPDSLPPKAKGEDYVYDMMKSLEERRLNSLSYEIQRIAGLLNVKAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNJ5</accession>
    <name>A0ABQ5RNJ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL6 alpha-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000936</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="14"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="102"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="193" mass="21862" checksum="325871966A74229B" modified="2025-10-08" version="1" fragment="single">RKMKQLLSSRTLPIPDGISIEVKGRAVRVKGPRGTLTRDFKHLAVDMFLTEQDGHKLLQVDCHFGKKKRLASIRTVCSHVKNMFTGVTKGFEYKMRLVYAHFPININIENVGKKVEIRNFLGEKRVRVVNMLEGVKIARSDGVKDELVLTGNDLENVSRSAALVSQSCRVRVLDIRKFLDGIYVSERGLLVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNW3</accession>
    <name>A0ABQ5RNW3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M3A/M3B catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF79">
        <property type="entry name" value="MITOCHONDRIAL INTERMEDIATE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="556"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="878"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1030"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1090"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1103"/>
            <end position="1115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1530" mass="160265" checksum="D76EACADE375411B" modified="2025-10-08" version="1">MQSLFSLYLGCKMHGAVGKVFDVRVECTPQPRPSSYKAWSNVVYFRCCSRTNDSTSPIVTNAVPEISAEGDGVGANLMVCNGKTYNRDIVSPSRRKPLVRDSFGPPGRHVGPLRAEPLQNCTVDTSATGTRTSLYARPGLLGMPGWHRPTDLDPWVDSVLCRAMQLQHGMSTCGPGLPLLALLHDHELLLRNSHRPVRYCVDFHPDPDWSTAATAALTRLDAAYDKLHGDAGVYEKLMATQTAVKVAMSVQDHRRTVGGGHPVKEGSCDLALGDAHVAAGANEPLVQLILHELHQHRGLAAAGLMQWLQLCRALRPLFRRNREEQQTAAAAAAAAAAAAAAAASVVATHNDNRKQAEPDVCAVQKAPFPPTPTPAAAAITSPQHKLVMSSECIRVCEDGSRDDDDDAADRQSVARNLSGGGTGGDRCYRSGDGSAVRSVRLQQLQSATARCSEALRRLAVGRPPWAPRLALHPAQLELLQPWLTGGDEGVADASATSPNGSGGGGGRIEWVTAVELLDLWTKRREIPDTSFGGAGGKGSTLTAAVAEVTPLATRAEAEAGPEDGCSGNGPGGSGGGGGQGGGAAAAAATAQHAGRRHLPLSSYDVAASLDLDELLFDATDEALELAAATADRKVSISGGGGGSSGWNIDVSSNSRSGGQQQLLPIVSLTPELMWRLLQLHPNEGLRWQVYHSGPLRQVQAMVAVLEPLITLRRQMAAAAGYSCWTDLIMHGGGTAAAGGGGGSALGGRAAIVRMLRELGQGLMSYGDSEVVALRRDSRRLGTGRTRAILPPGADPLDPWNLPYALQGLAQGQAASRYDTGWSSGYADYFTPPGLLQGSDTICRELFGLALEGPLQLAPGEAAGWIQEDLDPAVALDEHQQQLSEGKSGIEEKKNDGGGGVGGDAVMKALVVCCRTQRELGVLYFHLHGRLTEYPFTTLLRHGTMGRFTHVEGPTGHERIDMGTEFGEDHLPVILVRLPYRPDENSECGGPDSPYFLKAWLHELGHALHYIVSAAACLVTAASELLPPSPVSRQTTPSAPSSPSERTVPARKSSPQPSALPPSGDPSDMLVGPPFGMESDAEKAGGDSMKGYGGYGGYDGNGHDDEDEEHEDEGEEAVGGTHRWQLAALSGSSCALDIREIPSHLMEHLLRDPECVRRLSRHGRLDCPLPQKDCIRLVSLLSSCFTSTAGELQRLVAAALADQFMHGPANLTTTLQVTPPAAANADSDTGTQVAAVLKAVATLPLNITSSPLTTGCDGGDGGNAGGCSALRAHLEAHGSVCSLTAHHYNHNYQQQQQLWKGDLKDRDEEVMTPAGPPPLLVAGPDVVHHLEAAAVLGGVQYVYVASRLFAASIWRAYMQDHLPCGNLAAAAAATSLRRIDDSNPAGLRRRVAVGQGEKMNGGWLVRRRLLEAGAHSPGIDVLRGLLGHGAFVEMAVRCPAEDAVAACGEGAAEGRLEGLAATAAAAAAAGGATALSVEVAVGCGCNDAPRCVGGGGGGGGGFGLEASDACHCRCWVPDLGADVFQDVDLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNZ0</accession>
    <name>A0ABQ5RNZ0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF93">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="284"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="470" mass="48032" checksum="90514E225009ACC4" modified="2025-10-08" version="1" fragment="single">GGDAAIREMDAQLPEGTILLFRRMAHAMAQSQKPAASTTITTTAAAAAAALTAASSTTDGAQGREQIRTPAAGGSGGGGWVGWLMGGKRPPAAAAPAAAAAATAETGATAPGGSGCCKAGDAAAAAATAPVSATLTPEEWDRLQQLLLQEDVEDVDAPSGDSGAQGDGGDGPYCPSALLEVAVERCSVELDAVRAGVAQPLISASMQAVGIRILRYPTTLGVSLRVGWAGVEAPDGVLLSTGQLSNSNGLNKQPPPAKLSNTGSRYGAAAAGDVATEEVARMRVKEPETQDKGGSRPGTPAIPTRGSAGGNSWGGDDDDEPTCAFMLDFVRHPQDGSADATLSVRLAPSYVTYNPAAVAAVGAFFSTEQHIGFENLQVQAAARLQRLQRVAQVRLRALSQERQRLAVQVVMHAPKVALLDDKGLTYGSNEASLYECFDLGLERVEAAIVGGRFAWPGTRPSLPAALEEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP35</accession>
    <name>A0ABQ5RP35_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001166</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="155"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="112"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="501" mass="53623" checksum="1BC8F3356C9E5F07" modified="2025-10-08" version="1">MAFGAGEGFAYPEATADLPQPKSADPMPLPGEQLTRCDTPPTFPEPSRPPETRTPNRILSSAPSAPIDAETQNLPRPHQPCTSAPPHIKTHHHCTTSDGWRLHLVRTREAQTSTVTEASTPSEICGDASSPASSASAGCDVTASTNSVPPRQRRYPVILCPGLGSSGAYTFDLSPVVSLADYLASKGWDVWTVELRGNGQSDRPRLFVRSRWWTIDDYVTKDLPAVLQYVRHATGAPRAHVLGHSMGGMILTRLLALGGPPGEAVASATVVGSGCFLRGSWWQALQHTLWLARFLWLVPAGATLRAYSPLALGRAGLQLVDELYFWPPNTDPLLARALLARNFSAISAGVILQFGSGFGIRGLCTADGKEAYADAARLARVETPVLFVCGDRDRMCPPQGAEATCALFTGSRCARFTKLGPKYGTRDHYGHFDILMGHRVEQEVFPLLAAWLDEHDGADLDPAETAASSEGATGNTVAAASVATPGRFTAPQPAPMLRSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP81</accession>
    <name>A0ABQ5RP81_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000950</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="147" mass="15587" checksum="8AD63A406E137598" modified="2025-10-08" version="1" fragment="single">MPSIAGDFGPELVTLLIDGARYGDMEDVDSALAQGVAVDSKDAMGRTALHMACANGHCDVISRLLEAGALTDVQNDQGNTPLHWACLNGHKEAVRLLMEKGASAAVLNEAGRTPVDEALARDFMDIFELINSYNTAGAVSVEVQEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPB2</accession>
    <name>A0ABQ5RPB2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000634</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant tobamovirus multiplication TOM1 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040226">
        <property type="entry name" value="THH1/TOM1/TOM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009457">
        <property type="entry name" value="THH1/TOM1/TOM3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142:SF3">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142">
        <property type="entry name" value="TOBAMOVIRUS MULTIPLICATION PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06454">
        <property type="entry name" value="THH1_TOM1-3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="THH1/TOM1/TOM3" evidence="7">
        <location>
            <begin position="18"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI58882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="295" mass="33187" checksum="112B37EE83CD899D" modified="2025-10-08" version="1">MRTLHLAAASLGIGFPYPQWWRNLEKNESLQLYSNYGIGGAYGLIGLVAFFQLIRLQLRVPEYGWTTQKVFHLLNCLVSVLRCSVFFFRPALEGLHPVVTKLMLLDLPGLLFFTTYTLLVLFWAEIYQQARNLPAAALRPTFLIINVVVYAVLAGLWALTTFGKTSDVQELAYTISNLWLAAVFMAAAAGFLLYGGRLFVMLQRFPIESRGRRKKLREVGLVTSICAGCFTFRAVMIATSAVDRNDLEVDMLGHPLLNIAYYSLAEIIPSASVLYILRKLPPKRNPQGYQQIPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPC0</accession>
    <name>A0ABQ5RPC0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubby C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TUB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF7">
        <property type="entry name" value="PROTEIN KING TUBBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="3">
        <location>
            <begin position="627"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="569"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="590"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="712" mass="74194" checksum="90BE58AA67C9CFD9" modified="2025-10-08" version="1">MASNLAEADSIPRRLRAKPPPLDLHVVSPGVASIPNRIQFWPAAQQRVAPELGLSGPGSPTAIQEGGLSPGIALSPLSGEGPLEGGWAGLPAHILELILAALRDSKGRWPSRKVLFSVCGVCKLWRRVGYTLFFSSVWLNHTDVIVHPSQLFTLSPLPLRLHTSDHLLRCFVHRTPSTLSGLSPRFGRLWRFALYLGSDHRGLRCGFRIGQSSGGPNGGLGMAGGGLSANPQLLMLAQQTGRRSYQLLLSPATGPGSGPGGQGGARTTISWRSPRPGNSLSGLRPSEGGGHSGWRSPQSHISGDGNADRRAAGISGNDRGGGSRGDKDSGVVAVLRSNIRCSHYELIPKSSCPWARRLIAVAGYMPLQGGPAQPLIELEYRLRVRGIMLPRRMKVQVPVPHSLQRVVHNPLYDLHEPMADASPSASRWPPIFGANGEGGVAAAMAPPAPGAAGAVGAAAQPGAPNGGGGFLGEGQEGLMDNLEDILEAAMRTEPLLFADMMDAAVVRPADDDETVAGGDFGGRGGGGRVPSAAGAAAAGRALRRTGSTPGSLGSALLRGLLLNRHPAGGSGGGVNVQQAGGSVQWWDPGSPGGSRGVGDAGGAADDASKDNGAMGGWAAAPPPPTPTVQLQNKAPHWNDALMCWCLNFRGRVKMASVKNFQLMCSADKAERCVMQFGKVEEGFYILDFNPCVLTAAQAFAAALSTFETKYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPJ7</accession>
    <name>A0ABQ5RPJ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <ecNumber evidence="5">5.4.3.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_001195</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000818">
        <property type="entry name" value="PRK00062.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713:SF3">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI59336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="464" mass="49385" checksum="17CDA88642E587B3" modified="2025-10-08" version="1">MQSLHAKSVQGQRPFTAQRPRTVRGSVSVRAVAAPPRLITKRSEEIFKEAQELLPGGVNSPVRAFRSVGGGPIVFDRVKGAYCYDVDSNKYIDYVGSWGPAIVGHADDEVNAALKAQIDKGTSFGAPCELENVLAKMVIDRVPSVEMVRFVSSGTEACLSVLRLMRAYTGREKIVKFTGCYHGHADSFLVKAGSGVITLGLPDSPGVPKSTAAATLTATYNNLESVMELFAANKGEIAGVILEPVVGNSGFIVPTKEFLQGLREICTAEGAVLCFDEVMTGFRIAKGCAQEYFGITPDLTTMGKVIGGGLPVGAYGGKKEIMKMVAPAGPMYQAGTLSGNPMAMTAGIKTLEILGRPGAYEYLERITKRLIDGIILAAKEHGHDITGGNISGMFGFFFCKGPVSCFEDALAADTAKFARFHRAMLEEGVYLAPSQFEAGFTSLAHTDADVDATIAAARRAFARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ58</accession>
    <name>A0ABQ5RQ58_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mut7-C RNAse domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001648</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="entry name" value="YbaK/aminoacyl-tRNA synthetase-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052408">
        <property type="entry name" value="Exonuclease_MUT-7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002782">
        <property type="entry name" value="Mut7-C_RNAse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47765">
        <property type="entry name" value="3'-5' EXONUCLEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47765:SF2">
        <property type="entry name" value="EXONUCLEASE MUT-7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01927">
        <property type="entry name" value="Mut7-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="YbaK/ProRS associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Mut7-C RNAse" evidence="4">
        <location>
            <begin position="362"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="191"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="597" mass="61494" checksum="25A7B5CA4FA1A36B" modified="2025-10-08" version="1" fragment="single">PGVSWCGWWGRGWRWTRDAVFYRRCCHRHRRCRHRRCITVRRCSRNRCRNDTGGRVAFSRRSRHATEGAGDGGGGAVLQGFLWRRRQRWSRHEAVGCGCSQGRRSWRPLAGGSAVAAAAAAAASSGRGGGGGGGGWQVAAAVGVGRRALRVATPGECVSVYGFAPGTLPPFGHPSPLPVLVDAGLGIITELSKGKTEDSRKPPSSSGTITAAAAAAPPQPPQPELQWGSAAEDGRVSAAEAAVPASLYCGSGVAEAVVEVSYQQLLMLSAGRAVDIADDGLAAEAAAAATAAVAASATVPAAIITVSSGLEAVPGVPGARVTNAVTAVTADGGGGGGGGGGGGGTSVSFAAENVIVGGGAPTRFLLDSMLGRLCRWLRCLGLDAEYVGRTSAGKAELARLTARAAREGRIFLTRDQRLAERRDVAAPYVLHSDDAAVQLDELVRHFNVRFDEALVMSRCNACNAAAFQLISPREAARELVPPRVFQLVEEFWRCGCCGKVFWMGPKSASAIALVGGMLARSRRPEEPSVLSFFNDGYDMASTSHIPQAANPALASKLGAAAEVEVAAATATAVEAVMAGGTEGLDGDGDGADADADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQL9</accession>
    <name>A0ABQ5RQL9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trichohyalin-plectin-homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001694</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039986">
        <property type="entry name" value="CFAP210"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28663:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA- ASSOCIATED PROTEIN 210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28663">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="4">
        <location>
            <begin position="99"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="17"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="253"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="462" mass="52798" checksum="2C286A5ADBE75B9B" modified="2025-10-08" version="1">MATYSVISKAQLDKIREKVASPSPDGATEERKRLKDLSDARSAQWPNTLSAQRARKEKTRQERLAAEEAERVELDRQEAELRAEQRRIQIERANKILFDETDRVKAFHSKMLLSDVLHENEQLMEVKRQIEVLKKAQEQAFVEQQRQALEAAEAAELKKLEEARQRAIAQRETQLSQLEDLKTKILAERALDKREGELLKQKAQEEAEERRAKEAARAAKARQLAGETKAANAALQAFRLKEAERSKEQELAIEAYAKKKAEIAEERARREAEKRAAKEAERKRLADTMEDNYRQWNAKEEARVARDVAEADAKAAADAEARRKKAADLAAAIDASRQAQLRAKASAKAAEKAEEAEYVASWRNRSSQLQQEEDEEKLKRLAVGKQLAAFQLRQAAIKARKMAEARIAELQEAAQLALSVQEEEEIFLKYAAECIEEYRRQGKPTKPMELHLRKKTTIENMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRA7</accession>
    <name>A0ABQ5RRA7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="16933" checksum="80E7B90C56FE28A9" modified="2025-10-08" version="1">MNKHQRIHNGTNPHRPAWPKQSHGKLCDHLSHYSEKGKGPYNFVNLEATNPIRMSQAQEPSASLQQLQQPTDEPKVLGGGNMDPTNPLAGLENPEAEAKAIEMGASIASQGPPPELSEESITEPPSSGSQNDEAGGGSQPATQRETVQESGSQPAPGSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRC6</accession>
    <name>A0ABQ5RRC6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_002111</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18045">
        <property type="entry name" value="DEADc_EIF4AIII_DDX48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="10">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="58"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="239"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI60080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="400" mass="45673" checksum="04E6910A7286686B" modified="2025-10-08" version="1">MARNRAQDMEEDEEVKFETSKGVKVYNTFDAMGLKEELLRGLYAYGFEKPSAIQQRAVLPIVQGRDVIAQAQSGTGKSSMIAVSTCQLVDVAMRECQVLIVSPTRELAQQTEKVILAVGDFMNIQAHACIGGKSLGEDIRKLESGVHVVSGTPGRVFDMIQRRNLRTRHIKTLILDEADEMLAKNFKDQIYDIYRYLPPETQVVLVSATLPAEVLEMTNKFMTDPIRVLVKRDELTLEGIRQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRQNNFTVASMHGDMVQKEREAIMGEFRSGAARVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVRNDDIRILRDIEQYYSTQIDEMPMNVAELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRH8</accession>
    <name>A0ABQ5RRH8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_002091</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclophilin-type PPIase family. PPIL2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI60026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01923">
        <property type="entry name" value="cyclophilin_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16663">
        <property type="entry name" value="RING-Ubox_PPIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026951">
        <property type="entry name" value="PPIL2_U-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF1">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN-PROTEIN LIGASE PPIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="11">
        <location>
            <begin position="35"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="10">
        <location>
            <begin position="420"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="243"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="647"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="573"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="327"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="647"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="674"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI60026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="719" mass="75958" checksum="E438782F49714AF2" modified="2025-10-08" version="1">MGKKQHQKDRLFLTTKEWKEEWGGFKGATSAAPFKRLPFHCCAIHFTPFEDPVCTDDGTVYDITSIVPYVMKFKKHPVTGEPLQLKDLTRLNFNKNADGEFTCPVMGKVFTEHTHIVAVRPTGNVYCWEAVEELCVKPRNWKDLLTDEPFTRKDIIHIQDPLNLSGRTIEQFDHVKRQLTLDDDEEGMEGAAAGSSIRNVTEDMKRALGALNTQDAKAAFASGGGGKRAEAAKLLAEAKLKASGGGGAAAPRTPAGPAHDRQAMATSASGQAGPAATGPSSANIDVGCGDWRLRAPDRSQDMPTFKPGAVTWDTDDYVKPAAAAAGGRKGGSGAGKAKGDGGGATAANAAASASDANGSKPSPAAWYEAAGHVKYVESASTTGAASRSFTSTAWQPATRNERARTRLHRTPSKKGYVRLHTSLGDLNVELHCDLAPRTCENFLALCDMGYYDGTVFHRSIKNFMIQGGDPTGTGKGGESIFGPTFRDELDSRLVHAGRGVLSMANSGPNTNGSQFFITFKSCRHLDFKHSVFGRVVGGLDVLSAMEKVPTDPEDRPTAPVTIKGATIFTNPFYELEEEEAKAAAAERKKAEKEAAAASDALMNKIGSWYSKPSAGAACDPDGEGGTASMPVRSGVGKFINAALLSSRPQRQPLQQGQQVEGASSVTTRASDGAAAGGAAGGDGTRGKEEAETNPPAKKARVVAAAAATGGGRFGNFDSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRI0</accession>
    <name>A0ABQ5RRI0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002244</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1006"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1267"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1434"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1467"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1479"/>
            <end position="1492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1606" mass="164611" checksum="F87EE1EDEB3EC7D5" modified="2025-10-08" version="1">MSCMLRCFRVPSTNALASETTSPDMNAQVDIPQSSTHPHEIEPLSERSISSGKAQGSAARIIQAINDAFTKLGNVSGEHSLYCRISVLLEACRCELKAYDVRLAVFSTEGAAWVFLPLQSTEPDAAPAVAGWGNNILYRSLEGHQESVAVIRGYHAMALRSYTQATSPSPDAGRAQQWTLLSAAAAPITAPIMTSPTAVPSSIAAPRALSSGLPQADVHISHQGPARMATAPPLPPNTATCRTSPPEPCSVTHHSVLESVQMMPADMIAVPVRLEGRLAAALLVGWERLPGTAPLGSRAAASPSSPGVAAGMMKPKQAKSGPTPGGGGGGGDGGGGGVYYLGPTVIPHLQRLAQLFVFGAMCDLQQRRALAQLAQRFSLLPSAAGLHDFVTGFLDTVTELLYIKFSVRLQPIFLLTHGLATGGPAVLFSVRMPAPPGGQTSDVAAAAVSSGATVPIPQSQETYVTGPVVRLASGCGGNPLPSSSSLPGLRAEESANVVLGGGEGGGSSRVRAVRISVHHTLLAEALRAASKVTPATPATTTAAVAIQPPTNAADAAASTGAIAVNQYVGQGNTSTVVLCPLDPAAATTTTILGNAGGATAAVAPLPPPPSPPIHTTIVANISAHLLEEGAPCRDLLFCSNLAHCGTTGSLVLAVEAPSSSASGGGGGGAAMDTDRVNSSQIPSAAATAATAAGGISALSQPLGLLHSQRQFGMYVVCPDVLPQGMLAAIAAEVKQLLAMALSSCRDGALSVLAGGRCAWELYALQEQLLGINSSVAGGAGGVSMSLATAFAAASAASASASVTAAASGQALLTPGGSAAALPAAAAGSGSPIGIREALAPVAAATPSPLSMPTGRRGNPQGGRLLPRSFFAVVAASRHMSASGIRSAGCVRLPPSHMQLAVDVVTDGSSVVGRDLEQQLHESPPVANARLLNAATGSAVADQEGLRWLLASSPEVMLERAAAGPSPLEVMVSSMRTGLTAALTNAVVTSGGGDEARAALSQELAALRLYDVIGRGGQGVVFYGTLHGLETAVKVIVHRGKGEPLAHDGGDPRVGGLDEAGVVVGGGGDAMAGSTAAAAAQEDAGGDCPGDLSRIRKAQRGALELVVTGALSHPNIVQVLASFSNVIMVRCTYRNEPTPRLRLVAKDDPILASWTSPGPLNTVACLEYCDAGTLLEAAHAGAFRPAGSSILSGAVRPALVPLYMSLLEVALALRYLHSRRLVHCDLKPANVLLKSSTRDPRGWTCKLSDFGCVRLMTDLSVTTEDPQRQFLQPRRGTHGSGSSPAGLSQTHLNQQKQQQAAVVGFRVVQPLGTVAYMAPESFVRGHTLGPSIDIYAFGILMYELLMCRAPYIGIEAQALPRLVLRQHLRPEFHPLAPPEYCHLAARCWSSHVTRRPTAVQLVNEIEVLLADAQLKDSRKLQREATATAVPASVTAPAVNGARSPSPLQPILNSGGRKQPQQRPPPPQQQGEQEGAAAGPTSPPSLPPPPPRPQPQQQYQEPPVAELSQQPSTEEREDQQSCSQFVSTADPPPPAGRAAAGAATPLVAVLQYPQRQRQRQDLSAAALFAPAAIGELGPAAAVASVAGSLDATIEVLQLQLPSQPPAVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRJ3</accession>
    <name>A0ABQ5RRJ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_002265</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. Translational throttle EttA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00847">
        <property type="entry name" value="EttA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022374">
        <property type="entry name" value="EttA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03719">
        <property type="entry name" value="ABC_ABC_ChvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008775">
        <property type="entry name" value="PRK11819.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43858:SF1">
        <property type="entry name" value="ABC TRANSPORTER-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43858">
        <property type="entry name" value="ENERGY-DEPENDENT TRANSLATIONAL THROTTLE PROTEIN ETTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="2" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="95"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="416"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="40"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="647" mass="70328" checksum="549B885FE848BFEF" modified="2025-10-08" version="1">MLNAMLSPSLRSGRMAWKCGPRACRISSGRRLCVEINAAKKKSGPKTAAPPSTGPARGGGGSGGGGGSATSTKYKSPGEVRQGGAYKEETRKIILSLERVRKVAPSGKELLKGVSLGMYLGAKIGVLGANGAGKSTLMKILAGVDTALADGRIVTAPGIQIGYLPQEPELNDGETVSSNIEPALKHVRDKLAQYEALSLQMGEPDADLDALSNKMDRLQAELDAIHGWEVERTLEQAMDALRCPPPNGLVKNLSGGERRRVALCRLLLSSPDILLLDEPTNHLDAESVAWLERFLADFKGTVVAVTHDRYFLDNVAGWILELDRGAGIPFEGNYSEWLAAKAKRMTQESKQQAALDKAISEELDWINKKAKGQQKKGAARMRRYDELVSQQASYVKQAQLDSITIPQGPRLGDLVMEVNGLRKSFDDRLLIDDASFNIPPGAVVGIIGGNGAGKSTLFRMIMQQQNPDGGQLRLGDTVVPMYVDQSREALAADKTVYEEIAEGKDEVDLGGRLINARAYCSWYNFKGADQQKKVGSLSGGERNRLHLAKTLKQAGNLLLLDEPTNDLDVDTLRCLEEAILNFAGTTLIISHDRWFLDRVATHILAFEGDSKLHFFTGSYSDYDEDRRKRIGSGAAPTRLKFRKLATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS49</accession>
    <name>A0ABQ5RS49_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23823">
        <property type="entry name" value="RWD_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="2">
        <location>
            <begin position="9"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="261" mass="28503" checksum="9DCDD7910D20192E" modified="2025-10-08" version="1">MDYQGEQDMELEALQAILMDDLIEYEGNVPGGWIAAAKTYKVIIAPDEEDGVDSGDYPLKAELLFAHTPKYPEEPPSLKLRSVTGLSDTDLAEATAVLEEQVQANLGMAMIYTLIGAAKEWLQGRVNSGPVVDPEVERRKVQDAAERKRAEARAHGTPVTVANFAAWKIRYDAEVAAAKAKAAETLKDDDKAKRLSGKAWFMRQAAEGAAEDDEAGALSEDDEEDFDAEEVVAAGIDEEGDSDEDDIDYEDDDDEGMLDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSI5</accession>
    <name>A0ABQ5RSI5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="3">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002729</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reduction of methionine sulfoxide (MetSO) to methionine in proteins. Plays a protective role against oxidative stress by restoring activity to proteins that have been inactivated by methionine oxidation. MSRB family specifically reduces the MetSO R-enantiomer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173:SF57">
        <property type="entry name" value="PEPTIDE-METHIONINE (R)-S-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="MsrB" evidence="5">
        <location>
            <begin position="108"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="244" mass="26727" checksum="CFD755A6107ED72E" modified="2025-10-08" version="1">MKYFVGTRSSMRSLFGQRGSGRAVHTNAGPGPDYRNWALFRACAMLMSIAFLVLLAAPLTRRILLGAGITTTLVSVPRGPAAADGSEATSPSVVIDVEHRQGEVRFSDEEWRQRLSPEQYRVLRREATERRWTSPLNNEKRPGIFKCAGCGSPLFNISAKYESGTGWPSFFEPLSGAVTEVPDYSIVFMPRTEVRCRRCQGHLGHVFDDGPAPTGLRYCMNGLALQFNPSPGAAETTGSAQARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT43</accession>
    <name>A0ABQ5RT43_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TRUD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003012</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02576">
        <property type="entry name" value="PseudoU_synth_ScPUS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRUD" evidence="4">
        <location>
            <begin position="152"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="430"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="543"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="430"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="447"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="465"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="546"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="571" mass="61093" checksum="2415D4D9E7BB1D80" modified="2025-10-08" version="1" fragment="single">GKHSFVRFVLYKENMDTQVALTILSRHLGMAGGHNFGFAGTKDKRGVTCQFATAFKVSPFRLSAVNSHLRGIRVGNFSFCDDGLRLGCLSGNEFHLLIRDVRLVGPAPPAAAAEAGAAQGEETGAEGASGAKVEADVIAQVTMACETVARSGFINYFGLQRFGTGGAATQLVGKALLRGEYEEAVRLVLTGRTDERADYAEARRLFSEMGDAKGALRRLPHFMLGERAVLAVLATDPTDWAGAMRAVPRTMRMMYLHAWQSYVWNSSASHRIRTYGRDRVVVGDLVMPRSGTTAAGGAESLLLGGGVDDSGEGMESEDGLEGSAGTVSRRLALVRLVTEADVEAGRYNIFDVVLPLPGTEAQYPTHSTGAWIRKTAAEVGVDLDNPAITSAANRELSFASLTGDYRYLLHRPKDFQFGFKRYTDPYDDSLIRTDRDLLPKDPGSEKPPQQQQQPEPELQPEPGASGEGRGGEDGGASCGEATCIQPVGSTPSVDGPQPLLALELRFQLPPSCYATMLLRELMKTSTARTFHMGLSSTFAAAKSTGEEGEAEAQAVEDSEATAAALAEDVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTB0</accession>
    <name>A0ABQ5RTB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003048</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051836">
        <property type="entry name" value="Kremen_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269">
        <property type="entry name" value="KREMEN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269:SF16">
        <property type="entry name" value="PROTEIN SLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047361019" description="WSC domain-containing protein" evidence="8">
        <location>
            <begin position="26"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="9">
        <location>
            <begin position="84"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="9">
        <location>
            <begin position="189"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="9">
        <location>
            <begin position="303"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="979" mass="106807" checksum="46EE5F3919F6BE22" modified="2025-10-08" version="1">MSRMLHQSIFFLVTMIGAAVSGAGAAGRRSIQGPGSDLLLAPPNMSPSSNSSPSTLSPDSPTEPGSMAYQNLPLAPPPPPPYIDASFVGCYQDSWNRALPEMYDISSEMTIDRCRRQAQLSGFPYFGLEGGLECFAGNDILRVLTRERTDNCDMECTGNASQVCGGEWALSVYVTPTPASAPPPFGSSSGCFIGCFQDRNDSRVLPDMYEASDKMTIDHCREVALAQGYIYFGVEAGTQCFGANNLSRAHTNGQSAYCNWRCAGDDSQVCGGDWVISIYHTSEPLPSPPPPLPPSPPPPSLRKEDLVGCYYDTKDNRVLPLLLDASDNMTVAACRADALERGILLYGVEAGRECWGGDDLARATSLGPSTTCDWPCSGNDCEVCGGDCAIVIYTTELYESPPSPTPTPIYSIPPPYYFPSTPPTYLAPPFYAQPPSPPRAVPVFLPESISPEVAHFDAAVGVTSMSRASPFVGTWQDLNAAGRRRNKFTFSGRCTFTPGSLSAPPAILFGDQRCLGFLRNKLWNWGKSATAAFTLVWIGRFKKQPASEQVLLSLSPSWSDWDHGFLWTSKRVFSISLNNRHGQAWDYSNASIPAPLLSEQWVMQVLSRNPVDVGGVSMSYHLYDQSSGLRTWNLPGWPCKISPNAMAIGGDAHFNRWQFIGELSVLLLYNRSLTETDVLSLAKGYGSRFGWESAPVSPLPAQEVPLMPPSYSLVTPPPQSVVDFLPASLCPESAYFDAAVGVSDYLDAALDRSVVLLWKDVFVADRPDNLLWFSGECEATLMNSGQVSTASIRFDGRTCFATVEGNLPNWDVSGTSAFTAVWIGRFADDSAGEQAILSLSRTPQDFDRELYWTNQRAFVYSHYTGFSFDASLPAPITTGQWVMQVLSRSPNVESGDVTMSYYWYGQSGALSAWQFTGNPCEVSSDSLAIGADFRDGNRSFEGDLAIVLLYNRTLVEDEIQGLADFYRARFAWPKVGMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVA5</accession>
    <name>A0ABQ5RVA5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RBR-type E3 ubiquitin transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_003975</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RBR family. RNF14 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20341">
        <property type="entry name" value="BRcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20354">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23820">
        <property type="entry name" value="RWD_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047548">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF13">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="583"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="587"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="137"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="150"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI61563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="914" mass="97131" checksum="F959C4A0CA26148D" modified="2025-10-08" version="1">MVTQMGSARPDAETWQCQLEELLALQSVYEEDFRLLAAPGVGRPQPSIAAPEGITEQKHEALLEGGCLNLEELLMAGPPQELSTDTGGGSGDSDGNSGRGVFGCFVAEALVHVDVPEGGLQLILEILQRPLTAPAVVPPPLPPARERADGSGVVDSGSTSTRSNGGSTGLTDRRQVLGAEGNNQQVGGNGWAAAPARRRGGSGGGRKGDGEGGGHGGSDGGGSAFGSVASSVHGSGLGEVLSGRGQVSGRRGARGSSRMAPSSIPVRTGTSAVLTTDNNDRRSGAGSRGGGGGDHDNFQGPHQHQHQHQYQGRGGRGGQVLARGALQQTHAPVRDGVRSPAAAPQQTPVAASLKGSNVYGAVIKANENDVSATTSLGCALPGVNGGPSRPTQDVAPYKACAASPPRTQKTGAALAMAAERRGLETGTQIEAPGPAPPPPTTPIPIPTPIRVPFGATVKFLSPIRVTLTLPAAYPAGASPVVDLQALWLSAGQVQALISQLQEEWKANGGGGTPTLFLWLDWLRSEALPYLGIRQELVLSSLVTSLGVTMELVMAGSVAAAPEGLALSLVQYSARREHEKFNESNVSCPICLDEHLGQRCMRLPECRDTFCRVCLATHLRTQLGCGSVDNMRCPAPSCRRQLPPYVLQQLMSQAEYERWETLTLQRTLDKMEDLVYCPRCREPCLEDKDHCTLCPGCFFSFCALCEESWHPGSTCLDPEARLALLEERRLAASQTGGLSATERARQELNRRNELKSLALLATTTKQCPMCSMGVEKTEGCNKMTCAYCGAFFCWKCNQVIRGYDHFQQTLGGEGGCVLFEQQEIDRWNARWEAGPRAAQVEHRGGALVGWMRQNDREVRQCRCVVCGQANVKGGGNNDIRCWSCNSHFCYLCRTWLRSKPGAHFGPGRCKQHSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVX0</accession>
    <name>A0ABQ5RVX0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003498</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="8">
        <location>
            <begin position="1341"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="416"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="471"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="504"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="646"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="875"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="967"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1078"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1177"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="439"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="478"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="537"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="583"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="603"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1177"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1200"/>
            <end position="1211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1635" mass="173068" checksum="CFE4BEE5E61FE2B0" modified="2025-10-08" version="1">MWAFSCFCGFASANKTGDSKNFNISRNGIVSSAEPLSPSEAAALPLLDVSNLSTEWACSQFPVACIALGEQRNGSYVVPQVTVLWHNATSLDVWDRLTNAERNPALMEAIIEIINCSGPAQQVVMVAGSTFAAQPAQPAAAAARFTSELLAAVSVPVHLSLVQFSTGPQGVVVPRALLVVCRAPLLPPRHADSGPGSRQLRSGDTAYQGPLREQIAFHAGAIGGGESLRVTSGLSASGVTPEGELAQQASMHESTVAAHRQRDSMVAAHLPAIITVFTLEGQVVHQNPASMQYMGPMPLARPHGTVLLSSATMATEDGGLDFGGAEAGPYEEGDWGSGLGVLGCIFTYEQVKLRQMMDVLLGRGNVWQGIVRVPASESLKQKLVYGRHPHQIATMEDMEGQGPEAGAILSAGAYDTLQPRPWHPTLNTSQAMVPHRHQAQLQQQREQQQQQPEDVRFIQQQAQRERLLEQGQAIGQRRGSEDPQQRWRSSEGIEERQQLYHHQYQYQQQQQYHHQQQRQEHQDHGRQPSPDSRQQQDRPAYQQPQQSQLQLHQETHQQQEQQQQQQQQQGPHFPSTLLDDGAASTQHSSGSFMLSGSRNNYRNSFHNFTYNNNNSSYQHQKHQQQQHQQPESGATTGEVLETVLSAPVPAPGSGSDSPRPRGFTKSSATNAYDREMDPLATSTWTVLADPASRGWPLGHHQSGPRILTTGFMDHALRFRARRISKRAQSTATMLDSPVDAREGEVPAGQALSLDLTRRWGSATQTAPPPLGRRLQALPTGLGSGPQTGAMLGGLRHHSQPHVTSGLGAGAGLALARRPGILAPAGGSLDTAAAAAVVATAVAAERTGEPSMLPATATSLLGRALRPISLPLTRRVQRSDDGGSASDRNISTCASGRRGEGSLTVPNAGAAVNPAGNGVASGAGSNTTGMSRVLVFANSLRSAPFAPATVAAPVGSLQTGSRALYDASGTGGGATVSGVSTHSPRPQSATVIRRQSRSYERGLALAGMAATPAPAPPSPMPATPQARISNATSPTAVQMPPPAAALDRPPSPPPVPMELTRPEISALTALLRARRSTLANAAGTASPQSLPPSMTPNAASSTTITAGESAAAVEVATSATQPAMEMAVPHQARFQSSGVLLLNPATRRSTVGDDSGSVPTGVVITAGEAELAMALLPSSQLEQPDGQISNLGTSERREGAEDAAGEALADEADDAVGSRPAAAAFVAASGEHDLWHEISAVSALDPVTGSAVLVVTQVDVTAKVRAERHLVHVMCAERRLLLQLFPRHVLQHVVEANPLLRSHTAAGASSLQSLPREWLDGGDWRPVLRDCDKLATMHPQATVLFADIAGFDDICMELPPQKVLAFLNDLFAKFDNSIEPFGVHKMETIGGLFIVAGGLLREEEDGAVAVRDRWDPLHAEKVFMFAKAMLNVAAKTKLPTLEEPVELRIGIHTGPVMSGIVGTRVPRFVLFGDTVNTASRMQTSATPGSVHVSEDTRALLPHENWTPTGGVQIKGKGLLHTYVWVPEDQIHYLVPPAVSTPESTQTGGGGTGNGGAVTTYGSGGAPTAAAGGVGIAVANGSRHGMPPQMLMSTMDTSSCTSSDVFLAQQLSFTSAIPPSLLRGKSRLRNEGGYLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX59</accession>
    <name>A0ABQ5RX59_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004816</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="61"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="366" mass="40561" checksum="8E6A5ED656894805" modified="2025-10-08" version="1">MASGPQDIYEFTIAYKGPDQAVDYQLARNLYAVQEKAWEKYAAEKRLPNSRSKRKKLIRKGVPPNLRPWVWFEVSGAKQLMVSQPGNSYYPNLVKAAASMSKVSSQVELDLPRTFPGHPYLSCPTTGQVAMRRILTSYSLRNPKVGYCQGLNFIVGVMLMAMDRNEEHTFWLLAALVENICYQGSFGENLCGCHVEMKTLQDLVAAKIPRLGAHMKAITCDISLIATDWFLTVYCVSMPPESACRVLDALLNEGAKVLFRVALGLLKTAEARLLRLDNADSHHRLGPAGELMKWVKDFVASILHIEPIMEVAFDGIGRLSLSKVDSVRKAKALEVQAFMEERRQKASPHSNGASPGVGTGNRSTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX95</accession>
    <name>A0ABQ5RX95_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DIRP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004617</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033471">
        <property type="entry name" value="DIRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010561">
        <property type="entry name" value="LIN-9/ALY1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21689">
        <property type="entry name" value="LIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21689:SF2">
        <property type="entry name" value="PROTEIN LIN-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06584">
        <property type="entry name" value="DIRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01135">
        <property type="entry name" value="DIRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DIRP" evidence="5">
        <location>
            <begin position="719"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="91"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="241"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="330"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="395"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="481"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="532"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1045"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1273"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="976"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="405"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="487"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1067"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1435" mass="149217" checksum="4C222311B29BA1C3" modified="2025-10-08" version="1">MAGASPVGKLEDEVLRKFYRSFHKHGAPNWAKISKDTKLSVEQCEMLHKQHMSFLSLPASPAFETAFLAMVDDHYYATQTLVKDEVQIEDAARSGRQRRGEQDEAEAQVPIASSQKPKRTPKPTPQRLAAEEEAQARRHARANHGTPKSSRAKVVAKAPPSPPLGEWTSSQRKRSRRLFEDSAEVDASRYRSPVDRWRGRHNNDFDEGVDALLSLASGAHVASVVDEHPGGAEVVTNTADLDYDMPEPDAPEATQETPPKRRGIRGARGTPTPTGTPIKAGARPAAVAATTVVAAAAADAVPMPSTTVLRSPRIRVAASSAAAVAAAAAAAPAPLPMTSTTTASARPVSTAGIRVKKEPRGSPTTSQPRGGAATATLMPETADVGSDANAVTATQANESAAVPTGNESSPEGAGTMTTVPLATSPGPSGRPVRKRKPTAIALAAAAAAEEEDGVGGRGVRSVVAAVAAAVAAAEATQAAGAAVASTTPPPESAPASPPAGPSDEGPSTHATEMANNTAVLLASVPPVRTRASARLAVSGPGSRRAGERYSPTAVSEATGKEASPVEDDAGGGAGPGPLSEAQMLIPEPAPALAPIQAPTSGRAKASAGAGVHGQAGYQVAVPPAIRFGPARATNQPPAGRTRRRKLLPERLPPMWESLNVPALQAASGGGATVALDAAGSSGANNAGPGEASTAMSAAEIALRHCLGPRTRRWCMYEFVYCALDRPWFLRNELLEFCALHMQLPTTKLTRLEWSVLRAALGRPRRLSLAFLREERLRLEGYREHARIKYEEVALGMEVPHELPRQLRVGQEVTARHPHTRQLYDGVILTVKGSKYRVQFHRGDLMTEVIPDTDVMPLDPHECMPLHMAIVPFLLNGRPYDPVRLASLRGLLPPPGMALRPPTLPPRPLGSFATGLDTQMMRDQDAAMVAEVQRALEVKEDLVAQLTQLNNEAASGLHTDENGNRTDNFQLKYTNVVLKLRDTNAVLEAALSRLQARQQQINAAAAAPTAPVTFGPALALQQQQQQLVAAANAAIVAAAGTGMRPGDSGGVLPASTHQPQALPSQGPLGPPTQLPPLLVPQTPPAQSQTPPPVPAAAPSPIAAVLGSLPTSSTTLPVTGSQARPAANKVTMQIQPLSPGAPLVVPPPRPSAAAATASTAVPVPTAAGLPQFQGQGAPSSGPGMQVSNPEQLVEQALSEARTVVDSCRGKGTDGQSLLLQSGPQSVPVVAPIILQAPCGTSVPLVLPPGAILPQLVPQPPAAVDGQEQAPALTVAHPPHTLPADGDESIDGRHASAPTDCSSIGAGDTSAELAEPTSLAGVKLEDDPSLERMSESVALADRTVEAVPSACPSPTEQVMAEEELAERWLRDVIVGCVGVLFTIQKCTAGNVPASAVAEALDLAVQQLQIRTDGENDALYADIVQSVQGLKGHLTRAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXH9</accession>
    <name>A0ABQ5RXH9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="668"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="453"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="852" mass="89052" checksum="FE7FCB39A1C8B1A8" modified="2025-10-08" version="1">MLSALRGILQRWVGMTAEVQALEAEEAAAVPAASATPTCSTDYAANASRRLKRPGPSNTDATDLKRPRTAPTAPIDAGANGANPSAGKGSDPDGHIANPADLHPHHATNATRAATTASAPAALNRTLRPQPSVSTASPAAISSASRHIGAISGVVAAPQPQQQMQLQPQQQQQLCQSGEEGSNVNVGHDQPPLQRHQQQELAVQQEQQQREFAATAAIPAAAAVAASIAGSGLTAASGSSDTSSAGPPQGSGNGGAGPAIRNYHHNPHTPHHSDLHHRHDALEEQVFSPAFHLPSSSSPASSNSSPQAASRVGLPLAAAGGGGGGKPSQGNDDAAEAATMVRVTTATAAAAVVAVADGSQSVEVRASHHHSSECMQLHAIDLNVVDGVQVDTDDDELAAGAAAVTTAAAAALTAAAASAAVKIEDATAALRQGRHSGGGAAAAATIVAESREPGGSQQSGPPGKKGEEMDAESSVSDSGAAIEAVGEELSSSSSEEDGAKAEAGRGGGGDSHCSFDDEAEFLECDENDENVPISLLSGMGGHALSVMASEVGTIISIPQQQQHRQQQQQQLPGETGVDAVLVASAPRGLAAAVSAAVASAAVTQAVPQSVQQLQLPQQTQLPEEDLEEEDDEEGLMEFDPLVFIKQLPPLESCVPQHRPTLLPRQTRTMARRKTLVLDLDETLVHSSLEAVDRSDFSFPVVFNGTEHHVYVRQRPHLLDFMVRVSALFEVVVFTASQRIYAERLLDILDPHQQLVRHRIYRDSCVVVDGNYLKDLSVLGRDLAHTVIVDNSPQAFGFQVDNGIPIESWYDDDNDMELLRLLPFLESLAGEDVEDVRPRIRLQFRLRELIDQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXM0</accession>
    <name>A0ABQ5RXM0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004751</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="466" mass="49282" checksum="CFC3593774975D2E" modified="2025-10-08" version="1">MPLDFKEVAPDPKVKENNHKARLVALAERKGKGPAPLHLDSQVIPTLVRKVGDWKTGRISQAMCEAYLDRHTLVFDRELLTKMFTEGDYQKEGSLDTRALTIAISGRFPKREHTADWRALSALLLGLPDLVLVEDLEMAMLRRSADNPSRGTYNATSIWDSPPPPLPPVRRRTGSGRSSSSGRGKEPSTEWNDTMTRTAAAAAIAGLNASSGGGAAAAPTALASALGGMARSGSAGGALDGTGALAGGGTQATSALTGVVGTTGGLKQVSQIPDEQRLNASLMGTGAAGARSTFGTLRDFNDFARGLEVAPQLTADTSTVAGLGPGSTAEASSQRFTATRLGSPKDTVRVWAAPLPPSAISLPATALRTLRESVRSTISSKPEFGRVFKPLDSHDLDLKKTLGKDMDMEKSLARVEPVRDTKVLPQADYVAWCDYAANCRTSPHGWYSAHPAAAVQDAGDHKYPWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZG4</accession>
    <name>A0ABQ5RZG4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005023</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814:SF287">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="43"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="109" mass="12104" checksum="62716FBEC09373E6" modified="2025-10-08" version="1" fragment="single">INLAEIGCASTQLKLLYRKGFFHRSNGVFNLYTSVYDLLICKMTEVVLKVEMMCNGCVAAVQRVLGKMEGVESYNVSLEDQKVVVKGNVNPQDVLEKISKTGKKTELVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZH7</accession>
    <name>A0ABQ5RZH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MTM0349</name>
        <name evidence="4" type="ORF">VaNZ11_005635</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE PTC7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="88"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="389" mass="41596" checksum="51D5D31779C88A1A" modified="2025-10-08" version="1">MVVHVSGCHRRALVSHTVGSQTAMLSFFKISRRRSHQYSASSLPASSPNGLELADYPSTSNPLATGNLTTDNYARRVSLPPGKVLQLRSSVCYLPHPDKVHYGGEDAHFITDYGGGAIGVADGVGGWQESGVNPADYSRTLMQLARAYLEGKDIFEDLAASRHGVLVDPRGALEAAHMNTKVPGSATACIMQLDQSNSLLVAANLGDSGFVVIRDGRELVRSKPLQHYFDCPLQFGAFPEFVEATDTAEMADIYSIALRPGDVIVAGSDGLWDNCYVTEIISLLPKGPTDVQASAEAIATAARRHASDAEYASPYTREALSQGLDLPWWDKLLGMSFKGGKVHLKQLTGGKMDDITVLVSFVDMVETPTIPSSTACETPAGDNGALVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S083</accession>
    <name>A0ABQ5S083_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005428</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="518"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="571"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="729"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="822"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="608"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="737"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="824"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="916" mass="95090" checksum="E6BCD3633E782DEE" modified="2025-10-08" version="1">MLSRRSESCPRLARKEFTPQAQQPQQPLVPSTSGTARGCTLQDLGPEDRQKVAKLLKQVVDLGQEVLQLKQQRDELTQQAHASVQREQELQDGNHQLQKEHLLLKKKLAQVLLVLRASQAKVAAFEATQQGAQIAQCAVEVQTEPVSIVDPDMRSSISSNATACAIRSAATQLAQTAGNADLTGFKDSAAIRASLAMPAGRPPFLHVAPGAHVNITINHAPEPVTTAGAPTAPVGGGPVAQTNNPMDAEEAESITATSPEAGCNEGVASSLVPHCGASEYSVALVASPAAPAVIAIPTTTTTMTTMTTTAAVLGPVGASGTASLPRKELAPPEVSMGSCSAVGAGTLHIWPLQQTPVGMEAHDCVLGPAAMPKPPRHASAASSGQSICGKPPCPRNILVDATQRFEAVPVHEVLGDSREALTASPMIVPPCDEVVAAASRLLDWQAQHGLTVADEVLCAATAPSTISRDSRSGRKVLSYDPAIGQSGAFYFVDVPDMAADPAGSLGVLVSGATQAGRLGGGSITSNQPVPRPPHDAAPNAMPAAEKQHMAGAGPQLTPQALVFEGEATLRPWSDGHDASTSSRLGCPVGSVRQRHPNPHLPQHQHQQTVSERTTPASPCSSYTTETTCTPLVTMPPQLSGGNDTPASSSMSLSRMALALDEMARRQRWDVQPPGGLGQPHDAAVLGWTAHLPARGGNATQLAAPGAVVRHGGLGYGDFSAVGATGIGEGNGKRNYPHSASGQPVQSLRHGSTGSITVAGASTPAAAAATAISPLHGVRDAAPQVDALSTCGSTSNWLGGNDVSKRYDDSLVDILSQADEELEYQQQQNQQEHNQRQQQRARPQAQRKPLQASAAAAPLAAAMSLRSRAIPAVVPISLSEMAPTRAKQAASAEALLAANLYEENDVLSVILEQEMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Y8</accession>
    <name>A0ABQ5S0Y8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005818</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="223" mass="24390" checksum="4DB20E0C61335FAC" modified="2025-10-08" version="1">MRMLARQIKPFGTRSPSPRSLKPNTKGGRTCCRAQVVLVQANAKADAILEKAKAKYKEGDRLQAMKLYEDVLNEEPTADQKRAALFGVMAVHAAFGDVELAQMTLREALRNGLDYEKALEDPTYVQMVTSPQITIQLRRFSQQVKNAMANRPPTERPYGSGRSRPTGGQDLDSILGSAGGDQAEVDTSIGGIARRVALVVLAGVLLGTALWFLGLEYLFPKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S146</accession>
    <name>A0ABQ5S146_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006444</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052750">
        <property type="entry name" value="GH18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42976">
        <property type="entry name" value="BIFUNCTIONAL CHITINASE/LYSOZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42976:SF1">
        <property type="entry name" value="GH18 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="3">
        <location>
            <begin position="89"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="534" mass="56176" checksum="D173AEA06771875B" modified="2025-10-08" version="1">MHRGLKSRKHPRQQHAQIGARSRSRCVLEARTCKEVPAGGYRPSIRMSSASSATLLLVAAVAAVVTASPVVKRAAVEDTGRRFLGDACDNYCAGKATDLYANPCDASCQSFISCSNGLTYKMKCAAGTLYNPTGHNCDWPWNVVCSAKPSPSPPPKTSPPPSIKPPPSPPPKTSPPPPIKPPPSPPPKTSPPPPIKPPPSPPPKTSPPPPIKPPPSPSPSPNPPASGLIFSPYKDVGINLNWNTYTISTSVTGAQQPITQVMPSGLQVLTWAFATGTCDSENWAGMTPSSIISANVNAFVNAGKKYIISTGGAAGAFRCGTDSGFSSFLLSYKSSSLIGVDFDIENSLSEAEINSLVQRVKTARAGAYSNLRYSFTLATIASAPTGNQLNALGTAVMNAIKAAQMGWNGIYINLMVMDYGSTSSCAVSSSTGRCDMGQSAINAAKALNSSWGVPFSSIELTPMIGGNDVTTETFTLQDVTTMVNFVKQNGLGGVHYWSFDRDRDCWPGAAQSTCNSYGQAETLGFVNTFLSALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S148</accession>
    <name>A0ABQ5S148_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="642"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="751"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="891"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1034"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1280"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1527"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1728"/>
            <end position="1812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1873"/>
            <end position="1915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="655"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="756"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="904"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1075"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1129"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1227"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1399"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1417"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1459"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1493"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1729"/>
            <end position="1746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1875"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1885"/>
            <end position="1904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2011" mass="206421" checksum="1430C025C438FE39" modified="2025-10-08" version="1">MSQSHVQDTEELLRQTISRVQRLTPVLRWPRVAYQAKLNFYGTFYASFYDTASNRRLDNSEAGPSSAQPGSVSPATGEGSPTPGQQSQQQPQQHSEQPSTASPTSTERPSPQQLQKKAAGNGDASERLRSGGPIEALLVQTQQQTQQSQQQPRKGPRGFNIQRPTVRARAAAVHPAGPTTHGAVGGPSPDSSQSAHSPDMKHHPHQCLQQGRIVGQAKKQPPEPRTERQGLPAVRAPGPEVQGTKRTSSPELATLGDFCGTAQAGGAAVTGRTALERCPAVKVNTIKPGTRGALIDDCAGCSGDDDPQWVRTHISQGQPWHEAEEPATAGCSGSHHERDDAMDGPATGDPGVAATTATSLKLELSLNLAEAGELLPDAFGSLVGAVKDAHPQPGCSVARLCPGGSPRPEKATADTNPTSNSTDLQVCASDCEPSGPTTCNQSMYISMRALAQTAPCMDVIDDGRDVRELPAPACVSGLGTGATLSGIVHMDSAACGIFESAAVMGAKPHVEMERGYDEELRTSDPRFTAGWVSRPGPACDDEDIDTMLMQALDGWGGDGRGGMGTTGSAAVAVPLAGVEAGAAAAGEAVAGLSHGLLRPDPYLGSSDAGGGRNILTIPEADSGAESPPYVAVCVMGSAPKPTPVGAAGAKSPAAETREDLHPLRLDSPDDGTRTSEVGLKSLSKVDAADIRTGDMVGPSTATCTSADVKDMSTWRLPFGNVASTEATRGEEPAVTVDVATIEIVETAGLAGNPNAMPMPPPPSQPPSQRFDGIEPPVASSVKTPAGVSVSRRTSNISLQTLESGELPSADTPVGGTPSSALQPLPFQVVMTSTNSACLGVASGLPRAGRSSSLGPLQMLGRAGSGGVGSGASSRLGMGHLESGGLRVSLSEVIPPRVAEAPGNPEERREEGADGRMDAEPNVGPDVTGKRSPVLEPQPSNCQDGGRRRRWGAPVNAMMAAAGASCDAVGGRVMDVAGDGGGADTAQLGKVSQVDLSRAKGLVAHLSVHGSLQDVVPHIQAMSAAGEAFNGNMSGRMGLKRSRSPDGWAEHRLEREHDRGKERELRTSRERLDLQAATEGSAEANAGAGARRGASAAARSRSRSRSRERRRHSRSPRWRGSRSRSRSRSPGRDWRDKRNRTRAGDFDRHRDRDRYGGRERERERERDRARDRDLDKRGGERNDNRYENRHGRSQDYGRSSDRVTRDGRSDGRSERGASGGPTAIGGRQAVEVQSVSQQQQQQPCQQNPQQIMQQQQLQRRIGAATGTADLRAVMANGGAGIRRTAAEVPPRSESAGSVYPNKRPHRETGLGARNPGSTSRGGSGSDMDLDDSDTEGAGARNRPGVDATGTGVMGTGISATAVLPPPPTKQLPATSTSLGATAPRRPGGGIGFGQRHRSKAAAAAAAAAAATATAVTHVGSSAHSPSSTGGLSPVRTGAIGAAASPVPALGGSQQNQATSPGRPRSGTAAAGAAVAPAPGSVRQLGGGKRTPGSQALPSVQQPQNQQSLQPQQQGAIAYEGKVQRALAKLHQDRGRPQQGSAGGSGTPLASDAVPKPRPGKDGAGGPAASSGLGTSVLSDAGAVLDPIGTARVGGDVPLSSNMEPDLLGFMALSMIQFSCTYMVESPRLLFNKAHIKTLAEESRTLRRKADSNREASGNKWSTLQLTLYLQSALKSMESAFGHEQAGQEREAGLRAALELLSTASQMLAVVSHEANRLAQQLLRPPPSVLSATAAAGGDGGTSAARGSGMEGGGVQPHGRATAGALPSTPPRPFKTAPSSPTHSARSLGMHTPSGLGTGAAAGGSRTPQHGGGTPLGPLAAAVACASVGGPAPSAELLVLEAIRLLALKLLAICRTRWLLVNQDSLAHAIKLHESSSADGVGSSAPGSGGGGGGGGGDDGPAGTGGRRTSPTAAADSASSTLEAAALTASTYTASIQQMAEGVQNIAAAADAWRRAANEGRAFNHAVSAPGVDPAAVVAAARIGALGYEGGAWDLMGSVNLARKALDSITGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1H7</accession>
    <name>A0ABQ5S1H7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 157</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14845">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING 166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14845:SF0">
        <property type="entry name" value="DUF4515 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="51472" checksum="466C592785EC7579" modified="2025-10-08" version="1">MADEISTELTATQYVILAENQRKKKALAETKSQFSKVADENRVLLKQLEDTQRENYQVTEHLRRELLTKTEKIAALEADLIKLAQDKDAEIAAYKEASDKEKAKIRSDGEARARELQATIDSLQAELNNVLEFKERQAEVEEALMRLKEENQVLTEKLEQQRVELDRYYFELNTRMRKEYEQRLEELKKSAEEEVDERLDASVKRILGQNRRMAEELKIHVQETEALQAEVRVLEEARARLTREVGLKTDIEAGYAQRGARQAAALKESQARISALENSLQQVVQDFDRERQASTKAAAAALADVQAEAEALRRLVKLKTRELKNVRRLAQEVLLQRSDVEAFLLSSLHAVRREIERESLFPRQPNDTSGGPATAATSISGAVSQATGGGGGSRVASDIRDLPWEDRERVLRLLFAKINNQAQQVHFTSLPQHPLSSSRTEALAGLMTEAGPAPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1L7</accession>
    <name>A0ABQ5S1L7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006337</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="143" mass="15756" checksum="BA13465CDE93D68A" modified="2025-10-08" version="1">MHLPDSSRELLTEGADPRINAPVIAINRMRKGAVRAMVAQVRRFRRIIPGTRPWWQAAAAGLLMRIPFLLLAYAAGYVQGRRAQRHGAIGGAAGLKSADRGEQSMEKSRRGSDAGYGPRSRLGVKLLPFWPIRKSRREAAVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1N8</accession>
    <name>A0ABQ5S1N8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006702</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05379">
        <property type="entry name" value="CAP_bacterial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31157">
        <property type="entry name" value="SCP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31157:SF1">
        <property type="entry name" value="SCP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046770825" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="677"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="735" mass="76162" checksum="29DB9D06852955DA" modified="2025-10-08" version="1">MATAAARLPTLLSLLLSLFLFSMSSSGKSEDWAATAVSYDMPSVTHMENLVQAADMGTSIAADAAAAGQDEAAHRRRQLLESACSTTDDSAAAVLACTNAVRVNPDIMAAVVPISCASAATLAALRNPPRLPLTSSKALNNVSVTHNNKMIAAKKLSYQLPGELNMGQLAQAAGYNWYSLSMNIALGPKSARDLVALLFCNPANRDLLFSCTVMDIGVASGEGFFTQVYGCNNRFLCNGCGTPFPTTINTSPSPRPLSPPSQLSLPSSPVLASSPSPPPPPSPSPPPPSPPPRRPPPPLSRPPSPAPPRPSPPPPPKPSPALINPPQRSPPLSLLKPPPPQRSPPVTKPSPARPLPSPSLSSPKPPQRLPPLSPKPPRAPSPPPPQKYSCSSTDASAAAIMECVNAVRLNPALMSDMPCYASVVANITSPPRRPLMVNSHLSAAAASHNNLMVTYNSISSVFPGEPGLGDRLLNNSYSFSSAMEQYAYGVKNAYELIRWWFCSTGNRGVIFSCQLTEIGAASAGGYATQTYGCPGGICNSCAPQPPPAQPSPSQRPPPVRPSPPPPSPSPPPLPPPPSPSPTPPPPPPSPSPPPPSPSLPPPHLPPSSPPSPSSSPPPLSPPIPKTPSSPRSSPRSPPDTQLPPSQSPPPLSSPSKQPPSYPVLPTPSVPLQSGPQSPSPSAPPLSPSSATPQPPVSPPVVRNPPPPRPPPRTPPAPRSPAPRFPPSPRAPPVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S247</accession>
    <name>A0ABQ5S247_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006358</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="295"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="456" mass="47584" checksum="88AE9D87CFA0D9E2" modified="2025-10-08" version="1">MANYSEDSASSLDDSEDLDDIPLSLAPNGRHEHDEPPLTSKGAPLGFSRPVTGFNLKLPAPKADELGSPDHDGLQLMRSEPAYLAETARARPPMALSIGPGRVASTSYNGVPDQGVSFLGRRGSLKPPMLSLANPASASPVPSAAPTPGVDGVAAAPPHVTAWPVPEPKRRQSVRVDLVSSAFVLQGDEPTTPGETSASTYTSVAVTTGAVTSASECIAQRCSSQLGIAPCDLEFFELRPLESGAIPESCTRVGVLIRGSTFSLSALEDLLARSKRDSQLAGMLEGAIRDSSQHAAEAKQHAEKLAKQNEELAVLRERLRHVEAVNQHLRDQLQRSDEHRQLLGSTIRTIKKEFEDFKSRVVVEGSLDQLPLQQLSLGPSTTAATAGSVPGAGPVGNPFGLQGQAPVAFPGLLPGTTRSGSNGGSIGDVAAVPASASVAAVVVAPVPATREDGLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S253</accession>
    <name>A0ABQ5S253_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aspartyl aminopeptidase</fullName>
            <ecNumber evidence="4">3.4.11.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_006932</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate.</text>
            <dbReference type="EC" id="3.4.11.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the peptidase M18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.21" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05658">
        <property type="entry name" value="M18_DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002759">
        <property type="entry name" value="PRK02813.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF3">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI63830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="509" mass="53999" checksum="B6A6DD21A6CC796F" modified="2025-10-08" version="1">MASPGGDAEALIDFLNVAWTPFHAVEEASKMLLAAGFKHLQEKDKWDIVPGGRYFFTRNATTIVAFAVGKKYVPGNGFYMIGAHTDSPCLKLKPVSKSTKSDCLMLNVETYGGGLWNTWFDRELGVAGRVLLRSPDGLVTQRLVKITKPIARIPMLAIHLQTASERSDGFKINAQTHLAPLLATNTKAKTKAAASTTPAQAAAIAPAAGSDDSAGAAPPPPPERHHPLLLSLLAEHMQCSPADILDLELHMCDTQPSVIGGACDEFIFSGRLDNLAMSFCALTALIRSCGSGAGEGLIDEGGIRAVALFDHEEVGSGSAQGAGGPVMRDTITRVARALSGSEEGAAERCLRSSFLVSADMAHAVHPNYADKHDPHHGPVMHKGLVVKYNGNQRYATNTISASVFRELGSAKGIPCQEFAVRNDMPCGSTIGPILAASLGCLTVDVGIPQWSMHSVRETCGTDDVSYAVRHFEAFFAGVGLMLANLNVDALPPPVLRGEIEDPSCHHCTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2U5</accession>
    <name>A0ABQ5S2U5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006837</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046422566" evidence="3">
        <location>
            <begin position="21"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1362"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1367"/>
            <end position="1388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1410" mass="146416" checksum="AF912E82EB845209" modified="2025-10-08" version="1">MLNCLTYIAFVYWAVATVHTVLPIDVAVCGADVSRGGLGALFNHAFGGDLDVDVESWRGFPCKSGLLTKLGFILRRPAATVRHIMYGGGQDEMIVPILFIVPLIFSTIVLLRHLGLRLMSDSAVRILLKFGVAVGCRFMILLQSLANPTKESVYYKRYSKVEFFIIAWTAYIIELPMWHEAVVSGGTAAIILLLCTLFPHHMLPEVNPVNVATQAVAAAALGVGHAYYQQRRRIAECAARAARTVAKAAAAAMEREEEERPKQQQGLETQGQAGAPAVTDPSTSASISHHVAPATENLSNVQKSVDMSDGATGGLGSSPPPVVRTAAARATLSVSASITVLRHLHSASARPYVSPARLCRLSMKVQHAHLADLPPNLMAVINSEVLRRSGWQAISAHAREGCVELVLDLRPLVAARPSAYSNNDTLADDRLALPDQDQADGATSVDGGEADAAAAVRMDTDSLRELGRSIVSYLQSTGVIRPDRERSVVLQMGTDTVMLTWQPDTQSWVATEDADGESLSAAAAAAAPPLPPPSQLPTLQHISPRVVLWQGSASSRIHLTALIAGPSAEEVLAGGGLVVRCSARTLPYTVLRVRPQDTGVSVVAAAERPLPLPLVVDLAVNEAPGSGLLLVEARTSSGRLSPPAAVVVHHDTAVVSGLAALAAAMDDVSADAMLTDYGTWLDLAMALTAAATNGAQHDDDVAGDDGGKGASADTTTAADGVLQGTFSGALFPSVHGGPSAADASSYCTMSPNREVQNQAIRGRAWSAGGADGGVRISAAMRTIAADLIGVCCRGICGSGSGGDVSEATAIAAVVDHLIACVIACDDIRPPADALTALMDAVQAPPVRAGALHMAIAAGLRPVVEVIVGWYDKLCGGAAVKDLWLSRASCGVTPLHVAAAIPDGGAMAAWMLFKYDTAAAMWDAAEAASDDMLTPAALAVQLGGRWWLRPCRRVIGVVHVIKRALTPPVPLEHAAELLWGFRDPPLERRFVEALSGECVTALALYAAANTLLSLLALTKAVLYGNFEVTTALATLAFHVPQAAPLLAWICRNGNGSGRGCPLDTDDRGSVAASTSATAYATAVTAPTTSETTQKLSSSEASPRPSRPRPYSYLWRDIPRSLAAAAAAAAAAAAAAAALRSQSDGGRDRIRDRWVVGSCPVPPPPTHRVLDGSRRLRIALLVMHMCRAAAKAAVGLSLWPFSLPRGAEVFLARGGDIALEAVVRPVLEQVCLRTRLLVSLVDAPAMLLLYRGLQRKHPGLVPWWPLRRLAVYQAVLLATTAAVSATSRVRFLRALESPTRGVAGADPNAAPAAAGAPAAVGSSVSPSSMATYSGLSAGGGVGRGGGSASGSSFGKAVWRSRVSGKAPGTIGGRDCGSGGSGGGGGAGGLGKDLETQSTGDGIPRKPLCGDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S362</accession>
    <name>A0ABQ5S362_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006787</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="53"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="537"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="623"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1215"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1273"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1298"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1326"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1798"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2112"/>
            <end position="2189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2347"/>
            <end position="2407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2433"/>
            <end position="2465"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1903"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="336"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="544"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="633"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1223"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1240"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2159"/>
            <end position="2172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2433"/>
            <end position="2443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2465" mass="247652" checksum="313431F19E552C59" modified="2025-10-08" version="1">MAVNESSSVPARLKDLKDSVKERFLGLLGIRQPKVLLEDEQEVFLQVGRTVWHAPRQLRIELWLSSLARRHSLVSGYQEYLSKDITREAANDIEKDVQRTFPNTRRFHAEEGQTQLRNVLRAYAAYDPEVAYCQGMNFLTGLLLMYMPTEAHSFAALVVLMEDRKLRSFYHRSMSLLQVQLWQLSRLISPSLNQHLEALGVVPMLYGASWLMTAFSADFPLPFSARIMDVLLADQCECALLKVAAAIMKAVEQRLHAMSDLEEVLGYLKLDVPAWEEPELHDLLAAAFTKPWTPRQLSILRSTEGAETVAQAMDRVMNMHEQLSAQTCEDEEGGEDGGSAGGGGGGDGEARVGLTVPRLQPPPQPSSSFTASPACGTPVNGGAHGTGSHGGAASVSPTAPAAGSAAAAAAAAAAADVKYSGGGATPSILDGVTSGGSGNTTCRKGALILLQGGASANSSPAAWAIPCIHPNVPASPALQAFRRAHMTCFQAAVTAEARYGGAISSNTSQPRSHGSYALLNGSSGGLEDSALAATVAAGDVESDGSPHRSYPDLHRGSDGCSPEEWGDFSAAAVRQQWRSIVRAAPGSTRHVLMSAEDLSGSAAAAGADGGPWRGTASAFSSYGGTLAAPPTPSSRESSSGSATQPIPVPAGRGGVHSNAATVGSAFAAAASAAAAACSSTVTAAIGKPPLPPRCVSLDRGGPVSGGLYSRLSVVAEDPLALQMGAVAPAPSPRGSIDLHLLGRAGSQQLEAYEQQGHLLCGTGFGAVGGSSGSSTAAVNGGGGGASCSGDCVSGVASAATGGPVLSPSAISGAVSPHGMSSPRVLAGWCTSGPAGGATSTSGATGSNTPTTGYLPLGFERAMSGSLLDVNYGCNIGGGAGLSSLPERSYSMVSPLPTVAALEAAVMPLIPSHPRPAGASSAAQLTSGEHCALVSLGHSLATSQGTTHQCLEQEKQQSDMATDIAGDILRMLSAVDTGNANAAYDPGVILGESMYADSPNKEEHKLVRTGSEHLRHMPSLSALADIAAQDLAKAQEEAERVRLELEAQLQLQRASERISWSTSTAEPMTLGSISVSATISCGRQTGGGAGPLTEVQLAEAMAEAGATAATAVAVSSALESAAERVGTSSSGGGSSGTASAVSAAVTSTEAASGSGTQSMGVSSGAAGSGGVGGVVFRRPTLYVDGIPMFDPKSLMADNHEAQVMFTPTVSPGASSYADPVLSGFTPTGDSTTPGERDAAGDGDGTAASGGGGGSSSSGADVSATTFDKSDVVHDAVGSSESGAEEAESVPSVSAVVAEAAPGRASSAPPECLSTPPPSGTDLAETERLAAQSGASAGGSGDGCDAGAEAEDQLVMPAAAASRTVEAEAAAVNGTTVQEELSTLPSASEWVDWSQAPPILAAPSAGIPFSQLPSVSASVSAAQSLNASPHCPETKALSLVDGLPSPVSLSLLQPMGKIMAGLDHDLIDMASPARPLTLPISSKQPRQWSPGAGAAAGSDEVAVASSCCATGTGLSPGAPAVPPVVAALQPDSVRPPGGSFSDLAAAGGNSCQTACTSSFSQQPPSPYGLLPTTSSVTHVAPMAPAISFASSTAGGGGGSFAGGGAMTTVWSSSVGVVVRSDVNLTHPPSDSMDVNMRIHSHGAVVACASSDESDRCADDDDDFGDVGDADGRSNSILGVGAARSATPGSPEACMSPKRKLLASLHDAASEGSLQGEPGAADSNKFCGDSCVARALPHVMAEIMLPESSTQDPDCSFAWDSPSSNLPVSGGGVAATAAAAEDTEAACDEYSLRLQLQLQPESAMGHRQSEVTGTDGKLTKGNLAPVTGGVGFSGVASGRGSWSGALGPLELGDGVVPDCSGAASRGSVIEQQVSLEMDMEGEEVDNSPFRAVLSPSLAHHIMSAYLRSEQHQMQQEQKELEERMAREQERLALEQQQVQLAPERQQQQQLSGSTAAPATAPVLLATSAGLEFLPEAVAAVGSAFETVATSAAVAPATAATMMPIIHSGPPIVTASTCVGAAGILGEQIVPNMPMRTAASGVVSPVLCSPGRPSAVYNGAYRIASAPQQPYLMQQTTISAAAAAAKVMTPFALSSGDPSSSRLTAELQQVIPPPLQPVLLQPDAPSPPPVPNISGSVADGVRSHASGRTSLRCTGSGDGDGISGMTSSGGAAAAPGHGAGSIGSERISASGAPPPVSLPAYGASESCTTASSAMGLPGATADVATSMTMGAASQQPPMGHVSCTRIGSSGGAIMQLLPPPPPPQVPLCTVRHEPASVPTAPPSLQMPLPLPPVLCSSSATVTAVAEGEGGGGVAACTPVANIASGCAAMTSALPQPPPPIWTATFDGIDNTPKADVVATGTEDGNGSNGPEPRTLSSAFSAEADTPPQEFAREGSGCDGCHGAPATTTGSSSFGAQEYVLNVGKGMAIDAAATAMVGVSQVQQQQQGQGQGKEEPRHQPLSPGRPWDVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3A4</accession>
    <name>A0ABQ5S3A4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007352</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046259854" description="Guanylate cyclase domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="1931"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="585"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="4">
        <location>
            <begin position="631"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="915"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1479"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1693"/>
            <end position="1719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1795"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1837"/>
            <end position="1858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1912"/>
            <end position="1931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="928"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1921"/>
            <end position="1931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1931" mass="202284" checksum="5FEB767EAD0AA757" modified="2025-10-08" version="1">MRSLRLFLIFIAAVELKVFVASQSGYYTSANYSACINQTVARIVCPGNQSMMYKAGDMATGVIPNCALPFWEPGTATSHPTVKLLHPNIVITNTTVIPLARTVSESMQYPVDVDVVDSVTWTKEPLREFENSSVGKREAWFLDPVLTADMADKPVAALQDMSQLVLQDRNLNWNGYGTFFRQFASVYNGKVVGLPVSAAIMLLYYRRDFFKAQNISVPATWEEVVDIAEKYNGTDLDGTGTGAWGFCMSRQPKCYNGYTFQTIQVPFLQAQGTSQGFHFNPDTMEPLINSSGTRRAAELYRRLSPFIAPTEPVACLPVSLAFVRGKCLMAVGTTLHFKYAMFNNSLNNITRVNGKTGTAVLPGSFEVMDRKTGQLVRCNPEVCPHGYWHTLANGTKAWVNSAPYAGRGAFTYAINRHREPHLQYIAYRLCARRVYADAMWPAVLNPIAETTPVRTEMVDPANVARYINAGYDGNDTLLFMKAVSDTMEHANIALNLRIYGALEYYMAVDTAAVALSDTNTSIGNILAAAQQDVVNQYSNVSAEWLRQRYWKTIDRQQPEPPPPDSAARPEITGTSGLDVKTKVGIGVGAGVGGGLLLFGLLTLAVVVLLRPAFLSARHGKWYVPYASPECTLVVTDMEGSTTLWEAFPGDVMQAALQKHDAIARRTLSRHRGYESATEGDSFIVAFPNVLKALRFAQDFQAELMAADWPAVLLQDSDLCRPVYATPRAAKDGEQGQLFRRSASLRHPLIVPIRAVDPPPSSLPSAASFAHVTAIANTLSTAAAAGPTSSATIASAVRKVGGAQLMPLRTLQAPGGGMTLTAQQKDHLLQLQEVKWSQQGSGGLGTSLGDVPEKYVADTTSIGSLGSLSGAGRLPSGFFRKQSMQSGAAAASGIASGGALGGVHQGLPRLLSQQSLRASPLSAPPRAHPAADDHGDDGRDFGAASPPSAAPLPLPLRGDSASAERPSVAVSEDSAADCLQARAGLRPPSNTAISQLPQQLLTKPSADEPSVSANLAMLMASDFDVANSAAATAATWSAVATNTAAAALPPLAEGFAAAVAGGAMAFGGVSVAAAAAAAPAAAGVKPGSVAGGGIGGTWSWGSEAASAWQILEGPSPETITVFRGLRIRIGLHAGVRPSELTRTRASSRISFCGMAIRMAKAVSDAANGGMIVLSDTAAGLLLGTPEVAALLAGEGTVVWHLGRVKLASDLRDVEMFQVLTPSLAPRLALQQPIRVRAQLLPGVLAAPAGNVALVRLHVSGMVLLRATDVMLAEELAGVLEALLVESLPLLGGYLSEARQGGAEGITVAFPDCLRAVAWALAVQADMLHWNWSRELLNHEAFETISVDSGFGPSFTEQLYGDSEPYNASAAAEAPLRNPPSKTSRSRVCDSPVATVSSILPNKSSFLSHVSKGVQSATAGTPAVAASGGGLSFSSQTGRALESGGSRIGMFASSFTSSFIIRSQQYLLRGSASRKLVSNVEGPVPSLLSPLQPPPEAPVEAPKRPDFDRKTSAKRITLQGFRRWGSRQLGPGEDFESPGNKMERGEAREEQANVAQVLSGRMEGTKEQAAVEASRPSPAEESFRLEAIMLQPAAAAAAATGAAKAPADATATATARQVPIAEGLEDATAEASSVNMLHFSHAAAVTAAPSSPCAVAAAECRGPDPWLATQEGSIAHGKATTTAVAAAAAAPSSAAAAAATEPETSRISSTSRTGPRGPVGHKILFRGPRTKAVIDFGGVTPEVCRTTGRLLYKGRMAKYMTKLMEAAQRGQVLCTAAVYAQAASAAPHCGIVMTPVGTPGGPGRRKGLASNSRSGYGSSKAPSAAAAAAPAAAAPAALTGASGSTAPQPTGAPTEMQEGTVAAAAATSRKVQCFLCTLTAPPRGVGGYMPPPLSLSPPPQHHDGKPLLLSENTSFHTVLRAGDSPHTRSKPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3F8</accession>
    <name>A0ABQ5S3F8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Replication factor C subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_007707</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex with RFC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the activator 1 large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17752">
        <property type="entry name" value="BRCT_RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013725">
        <property type="entry name" value="DNA_replication_fac_RFC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012178">
        <property type="entry name" value="RFC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25361">
        <property type="entry name" value="AAA_lid_RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08519">
        <property type="entry name" value="RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036578">
        <property type="entry name" value="RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="11">
        <location>
            <begin position="342"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="68"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="158"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="318"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="536"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1008"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1021"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1087"/>
            <end position="1097"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI64437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1124" mass="119629" checksum="6C9565C73D121145" modified="2025-10-08" version="1">MDKSKQKTPTKTQAKDIRSFFTKPGGSLGAAKDTPVIAKDENPPKGEPVVASPVALKPVAVPQVAQQISKPAATAKVKPLAKNSKAATLDDDDEDVVVVRRPPKRKEMQRASPGAGRSELGDGNGSGGPSSAVPRGDGAPVAKRRRVVVDEEEYVALVESSSDDDEFSVRAVRALPGRSTSETSQDLEDEQSSDSGNVFMETEDERAASKRKMAAAKAAKPATKLAGTKHARSPPAAKALTSTPRAKADVKAADAEAGSKKIARANGISAGGSARKPAASKKAAPFTLNADAEAAVEAVNKAIEHLPSEEQVNFSLMPEGGEAAGNDSTEPPNYRKKEAPRGHPDCLTGRTFVITGVLDSLMRPDAEDLIKRHGGKVTQNVSGKTSFLLVGHNCGRSKYRMAKHHKTKVLDEDGLFALLRASAPFAPPERPTAGPIPGTAEPASIPTSKFFGSTVAGAGGGGALRKPGDPVALYNAGVRIQPPITGASSGGGSLNQLWVDKYKPANSFELVGNNTLVANLKQWLECWEQVHQRGAAAPTAKGGGSKPKDMSKKAALLSGPPGIGKTSAAHIIARETGYEVVEMNASDVRNKSSKVSEGIAGKQSNVIKEMVSNTTLLGPLGGKIRKQLLVMDEVDGMSAGDRGGIQDLIETIKRSKIPIICICNDKYNQKLRSLRNYCMELEFRKPTAQQISKRMSEICQKEGLQVNQATMEALVTGAGGDLRLILGQLQMVRLRAQALTYDEVRGKMGASKDTDMSPFECARRLLEPSCSLLPMSERMDLVFADMDLVPLLVQENYLNHRPTIAPDISSRLRAIAKASDSISFGDVVNNTLRRTQNWGLMPFAAVMSSLLPATYMRGTREIFGLYPTEMNFPRFTAWLGNYSSGNKQRRLLGELSTTMAASGRISSSRSAVRLEYASALRHLLVQPLVREQEAAVPSIVELMHGYCIDREQFDFILDVTSFKSKATWAEDTFKEVPTKVKSAFTRNLNSSMPTARCLAQVEEVKLGRGAGKGKGRKAAPEDNDEEGPLGEGEGDEDANPMEKETAEAAQGEQEEDEVDPATLARRLAKGGLVVQLQDDSKAKGKRGNAGAGRGGGTAKAAVAGKASTMGRGGGRGAVKASAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3Q7</accession>
    <name>A0ABQ5S3Q7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinetochore protein Spc24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.570">
        <property type="entry name" value="Ncd80 complex, Spc24 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="21875" checksum="84D3C4A0F98E0887" modified="2025-10-08" version="1">MARAAEPFSEAVHYIKVVYQEYCKRNDLDTCDAIEQEISDLVHMCSTTEAEVRQTIKDLSKQVHDLETVASYPHRDGLHADRVAALQRQIDVAKRAIEDMDVQARTAEQQCEEVDARLRCATAAAREAAAASSSIGANLKSQLAMYKHITSTTWWPGKPTISGTVSDTKTGDIRVFDFDQNISQFELINKLWDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3R5</accession>
    <name>A0ABQ5S3R5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="142" mass="14753" checksum="8B90B2698F188DAD" modified="2025-10-08" version="1">MGEESYECGFVFLLGTIALRVAKGLKRKDLQSDMLVPFGPPQQLPEDSSVQASHGEPAGNGSLIVLSDLSLTMHGFPGLRAGVRAQLVLDGCGDARRRVRPEPAGAVSGLPAALVRAQALHHGDGPRGGVDGGRSLGLPPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4K8</accession>
    <name>A0ABQ5S4K8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="16165" checksum="36EDDBDB310405D3" modified="2025-10-08" version="1">MDGAVDQRGLLSPQIQDHASIPEDPTDEVVTNDHDLGAARPTTAATVMHTERRRGARGGSGTGARGSARAGPSSGPDKSKKRGGAPTAAAVQNSHPRAKRMRTKDPFLPKWDCKRPELERRKTANVCWRCTADHLTKDCPLLPPLAMSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4T9</accession>
    <name>A0ABQ5S4T9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Auxin efflux carrier component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_008314</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the auxin efflux carrier (TC 2.A.69.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051107">
        <property type="entry name" value="Auxin_Efflux_Carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31752:SF18">
        <property type="entry name" value="AUXIN EFFLUX CARRIER COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31752">
        <property type="entry name" value="AUXIN EFFLUX CARRIER COMPONENT 1B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="784"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="826"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="865"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="893"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="925"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="171"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="663"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="256"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="666"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="960" mass="98710" checksum="7B64413C408DB3A0" modified="2025-10-08" version="1">MGAWAFQNVLNVGIQIVLIVFSGWCFARFRLLTAEHFMGQINVLLLRVAFPMLNIYLLGIKVDLRDAEAWRSLGAYMMWVTLVQVGILLYVWLCNGHTIGDAGLLNLVLTANNTVIVGLPVMEATFPDMGGRLSLLTAFVLFLQVIPFSITAFEVEKWMVEDHLRATAAANMVSEPPSTPDRELSGRWVAEEPSSSGRSDGGAMEHRHGEGCSSSGGPAAEPPTPRASLDGADGRHGSSGRGADAIVGRARRRQSISRCSGSSTPGSSIGRDTVLVGGRLGRRRVGSGVVVGAMARGWAPATSLDSPITSSLDVSTRGKASGNRQVHSAALQLPLPAATSFGTLACPGMAPGHAISSLDHQHAVAPTSRSVALAFPDHAGSAPCPIVVNSNDGSRSDEGVVAARGCGSHTSTATVPTVDSPSVASFRCSSAPASIAASVAQMTSAFAFYPHLAATTASIRPNNYAMVTLTGAARSAPAVDPSGTSILLGLQTAASSPAFPEPLVTSALMGATTTAAGAQSHLGPHHAAAVPNALQQHLDMRLANSRALAGHCAASSAVDPVSGPVAQRLSGISGGTGGGGSGGGVVGATGGIQRWSLQAHWPPGTAVTNGRLEVAVADAGDLSNSATAPPPPLPPPPSGRLLSPVAPACPDLPVPQAINLARPMTSGPTVSTEARTSQNDYGPGRTAQENLVLAGNGCGGGNNDCSTRASVGRQLSTPAAAAAAEAGAVSLSVAPAVDAPPPPPPLPDRAFARAAAGSMTRRHGGMVPALGRWLKMRLTQYRRAWSITWVILKNPLLWSLLVALVINLSGLRSILWPGSSHYRPELGWVAGALSWTSGITVPVSLFSNGVWLYGKKFGRATLLQAGWILLIKLAILGPMQLACAAACGMEAAAAMSLLLLALCPVASTSFVIASQYGHGADLVTSITMLGIVLLVPVELVALALPRAFGLYHYQVRPATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4U7</accession>
    <name>A0ABQ5S4U7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">CDP-diacylglycerol--inositol 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 16">2.7.8.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">VaNZ11_007580</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11580"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16 17">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.11" evidence="5 16"/>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014387">
        <property type="entry name" value="CDP_diag_ino_3_P_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF4">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000848">
        <property type="entry name" value="CDP_diag_ino_3_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15 16" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GLI64342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="247" mass="27527" checksum="D9427E70DE1213D3" modified="2025-10-08" version="1">MGFFSRHSNVYLYVPNLIGYARVAAAIYAFGVAFTSPYQCVAAYFLSFVCDELDGRFARMLNQTSTLGAVLDMVTDRLATTGLLLVVALVYPQLHVVAICLIFLDVFSHWFQMYASLAVGATTHKDVKSKSWLVRTYYGNRLFMGYCCVSCEVLYLVVYASKWPELMQFVVPLPGGAGLTIPAQMANLLPALLRFTSQSGLPLMSLFGILALPGFLTKQICNWVQLRTAADQLIAFDLKKKPQQKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4X8</accession>
    <name>A0ABQ5S4X8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RegA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="625"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="941"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1323"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="946"/>
            <end position="955"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1354" mass="137362" checksum="6D77E7FA6DDE1455" modified="2025-10-08" version="1">MQGAALGPIGPSGSQNSDAKARPKANLQDLATAAAGLSAESGSSDSCGACIGTPRSLSTAGIPPSLTSGAAVAAPRPNAALSGRERDLPSSHAETPHAHTRSHRCSSDVLPPVESKYSMDRDELQRNPLHAAVTTGGPSPGGASMPSHINTASMLQQLQMLVARGIICGRDDGDLDGDKGGRDGGSTAAAAIAAAAAAAAQQDGANVAAPRSRGANLDGSYDSSFRLSTQPNGEPQSVGSFGGYGMPLYVHGSGKANAAAAAAAAAADVGGGGDFKAGPGRRPRKRSAATDPDDAPHDRNSPRGESFEGYELPQRAGHAVLAGDTATPPTQHHHAIMEGCTRDASTTDLAATEPYRRDPAGGEGDGGVDADGVGVPGATDDGGSLFASELPPPVEVTVAVRVGSGRSGEEDARHGNSSYVKGLVCGVFEPALYMNRLDCIRYRGHFISRSHFEKIGGSSMAKWYRSIRVLPDLEPLGEWLERHGMPVTKGPARRYRPRKQQGGGSSGGGAGGGGGGGGSAAAVGAVGVNVADGGVEVSGGAGEPEPVVPPKYIAGSGEVCSSSCGGGGGGDGSGGASGGGGSQRPRQQPGGSQGTALSLQHQHQTVITTGIQGGVVMRLQQIGETGQGASQGVKMEDVKIEGGPGRAGRELPAAATAAADGEPSDDEAEGTAALISLSMQGANGGPAPAAAWSLRDAAAGHGCAGGEAYSPKVLLQRRQQQLPPLPPPQRQGRVNLATRPLLYQGGGGAAGGAVGDYDVVESEGLDRTATERQSGELGGAGSYQRQVCGASRGGGGLKGDASTGRIAPQHNTAISALGHKDAVAALAAAAAARAEGPAAAVAGPPLQHPSLGVSSATAAVAAAATRLRGLAGKGESGDVEIVDRVGSRDARYMHEDAFGTEKVNGDVDDYGYYSERPWQRGGVIAPRGVVVDEQGMPATAARRMTASRLPVSSSSLEEEPPPMAEASYLAAAAAAAAKGRVVPSQFADGVPDSTLEGTAAQKNLVDDGLDMRYGDAEDTDAEDEEQLQAYLMSVGAPALLRSIQLEQRELLNRWRRLESMRHRLEDLHNQPRAGGAPKVETAVGRRAPAYDALLQHTAAAIDECPDRSRGRVGSGGAAATKRPRGEIASVAISGHRMLEQDMFGTGHGGAHILYEDNGGDGNGGGLRVLDPSRLAEVYASVAGGDIGGVHVRRGQGTEVAQPQPSLVRIVHRGQPVARGGEARELVLAVDEDEAYGSYRGHGRGRAVVCEELPPGTRLPPTAMAALRQQVWQRSHVRVEAPPPRWSRQHLRAGGGASGAGAVAHADELRAAAQYAVHIGGVSRGGGAASYGSPERGDEHGGVNAVAHGVGGNWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S531</accession>
    <name>A0ABQ5S531_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF11">
        <property type="entry name" value="LIPASE YOR059C ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="2">
        <location>
            <begin position="734"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1015" mass="107144" checksum="948B16E9F7FC3344" modified="2025-10-08" version="1">MSSHGDGGNGGAQVSTAAATRSGITGTHPTGRSSSGGGGNPGGSIRSTLQPQAINSYGSGGDGGGGGGVGYGAATSSSQTGRAAAAVGGGSGGGSSGRAASAIPTSEVDQALSRAGLVAVLNLCVTLYKFVNVELHSQGWYAVRVRVIPGSDAVCTTDMVVQSPQGPGAVGTGSSFQPIIGSLGGDGAQAVKGAAANNNRTPGCYADGAVMASRRQGWLVEESGCSFRSCVFRIRYCYEEVKLQTAVLCRIFVNNLQALSTLSVTLEFELMFQEANLENPSFIPDEFHLRSVARETVTLAGLAGGLAEYYGCTFSDEHFGLCSTGMFGGLAGFVTRDMVGSPSQRVVSSSWLCHLVGDSRREPGHNYRAEPNATASGLATGSSAPRPVSFVDSSSSSLAQPSGAGFPATASGRVALVHDSIDFSDQELRKKVIRLLHHVVEAPSLKVLEQGIPQIPSEAERLERLAAVYELLVEGHRRSRDRLGSFLRQVPSECYAPQNLDVLRALLASAAAASASASGPAAAAAVVAAAAASANQQNLLRGAMPRASAAAPLARLSHGPGSGGGAAAASDGTSSGGAEGAAAAGHHVVPTVAAFKSLLRHTPTTAAALQQLDGVLVGLSTDSRTDWAVLSWVMRTLPRELMRHLKLRWETAQPDVVLPFIVWDSTPSRDAIAAKAASHRQKLRTPRFRMPAVQPGSLWGSPGDQPVLVVDDSQGIKWLAECDSYFGEGFRLRDESHVAIFVHGFQGAATDLCLVKAHLMLMYPYLECFSSKTNEGNTHDSLQEMGKRLAIEMAEFLAPFAHSTRRPLRKITLVGHSIGNLILRAALTQPEFAPYKSLLWLYISVCGPHLGFLYGTNAVVDTGLLLLKSIGKGKCLHQLTFSDATQLTDCYLYRLAHECPLSVFKLVVVVSSPQDRYVPYHSSAITTCLQAERDSRRGRCYNEMLRALTAGVGGGTHLFRLAVDFSLRAKSFSFSKLVGRTAHIEFIESQLYVGLMMWGLVHRYTMLAPSPHSWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S578</accession>
    <name>A0ABQ5S578_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008448</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI65035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1053"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="382"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="607"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="752"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="385"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="607"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="655"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="752"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="1080"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1393" mass="143236" checksum="A3B4D91DA20DFA6A" modified="2025-10-08" version="1">MASCFLCFKSQATDRREHENAAPVLAPKAAAVRTAESAAASQPTWLPLSTSKVQIPQPPAGAASASVPPVVLQLQRKLAALQGTWISRVGMALNYTMECTGATAVSLSMLSQTHDCLVVLARRGPQHLVLPPGSCISITEPGETAQSLDNPEHHSLQHLYTSSAAVVRCVASSRQPTNALPSTTSVAAASNVAHPGNSPYGSESLPLDWAHLHATAGITHFAALPLYHGATLAGALTILGSNLLSSGDTTQPIPSAALASVNGGSPISPMSITPYLPAMGSNGGGAAAPVSSLDALFRAPFSLELVAMAVAQCCLGADMALARHAVEMVQAMHACASIQQLVGGISLGLQALMHARFCLTLRCNVALAVEFQPNAIMFEEAPGAQQPSGSGSQNNWNAYEANPSGPPSRAHHVTGRPSAAFSSPAPGPDGTRREAAHRMGSGGLAPMNGDAAAAAPGGGGGGGGGAATTATATTTTGRAAFNTMRSGVDTTGEPLWCASSGHVTASALLTQIVGRRYKARPFSVYHTLLIRTIHELATSSYGGTVITQCHDHMQDAKTPSRDIYALGRGVGQPPQSLVLSVTHLRPADEAIPDNALYFATGGNGSSGTRMCSAQSGRNGALSGRSNSHQSVAAAVGSGVGGGGPDVGDRSSKVVPPEASPENPSSSTGPTSISAHQVLSAAGTADSPVPSEPRTYIGLYLSSTEPLPAALLELILVEIHSLACHVLSPLVHRKLLAGELAVEWSLMQTVTTSSLPQGTDTSNPQGRTPRQNLQVAGGAAAAATPGAVQAMGQVAAAAASRGNASIDMSSLTVAAAAVAAAGSVPASTGSTLCAGQLTAGSTTPVNPDGSDTTPAAPLAARVSVATGGPAAPSGPSARLSSLQRIMAPFLMADMGVADGASGGGAAASRRSSDIAFAFSSAQGAAVGNGARSPSPGSTTNAAAGIRRILAFDAQAGKTAFLKHNRPSMSVSEGLAREMMLAAAAAASAPGAGGQAPGNDKFLSTEVATVQDTHSQMGLLVTSYKETLHNMHMELSIGGVCRQSLDLNADDPGRLVLREVLGKGGHGVVFRGTMHSLEVAIKVFQTPGEDEPLNAGTANRPEVLAETLLQRRRVLTRAALELALMSSISHPNVVQVYAQWPTALLERDETQPCRRRLRKLPPDTTPSPSGGLVCAVMVMEYCDKGTLVDAINRGDFVVPARDGSGFKANYKAIYTTLLEVALALRYLASMAVTHCDVKPANILLRSSPRDPRGFTAKLADFGYAALLRDSSPDGHRSVVTDEACGTVTHMAPESFVEGEPVDSSADVYAFGILMWELVSGKQPYHDRNLKQLPHEVVNKGLRPTFPPNTPDVYKSLAKSCWSPLPRNRPTAAQVVQSIQAQLDKIEAVARLLGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5D7</accession>
    <name>A0ABQ5S5D7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008419</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046220702" evidence="2">
        <location>
            <begin position="19"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="642" mass="70033" checksum="7F5CB68F7C5B326C" modified="2025-10-08" version="1">MKLHSIACAFVIINGLCAVESTDINNAFAKWLERQDEVQTGRYSHVTDASDLPGSDHNALLETFLMDTVANHVNSSLLEIVTGAQDLVQRDGSGFSEIVISLKNLISQLPENLRAWPLVRRIVIASLALRYERKGRFIKGAETNGAQSLLLKLFARHESSARNKGVVEFAHVSKSGGTTICQLARANGCKTESFSPNNNCLIAKFKDEPRYLDGAIHRALHGPLKTKCDNGFKTMRPRTEISCDERRRWLLRQGYTLYANEYTALGGQVDPGRAHACRNMVTLLEIRHPHDRVISHIRHVWHGYRAHCGEDRKVYFAGNYSVSHWSSLMPAPTNNYLIRSLLGQAVFNLPVGGITRAHLALARFLLAEQFDVILVLEDQEMFRQAMRYGLGWGLHERHANPGHSDPEDGLPKDLPALWELNALDTELYRFGSLMARLDAIMYEVAQGAKEDAPEAIGLAGTSRGQVQGLVGVTSLLAETTTADGSSINGSEMATGDSSELGPEQQEEQQDGNIAEKRVEGVDMLGAPQLVSSVPTAPTEGGSSKRRRSLLGRRLMTASSVASRVAGGSGSEQALIGSGLSRRDPEAMQQKPHSSAQTRPRARSPVTALQSPGFRSALGPSLSDETWPVCGWVGAGAVSNGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6Y1</accession>
    <name>A0ABQ5S6Y1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">TPX2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_008529</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPX2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027329">
        <property type="entry name" value="TPX2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027330">
        <property type="entry name" value="TPX2_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009675">
        <property type="entry name" value="TPX2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326">
        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326:SF44">
        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06886">
        <property type="entry name" value="TPX2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12214">
        <property type="entry name" value="TPX2_importin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPX2 central" evidence="10">
        <location>
            <begin position="256"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="TPX2 C-terminal" evidence="9">
        <location>
            <begin position="518"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="TPX2 C-terminal" evidence="9">
        <location>
            <begin position="566"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="115"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="196"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="635"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="586"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="74733" checksum="E35987BCAE581600" modified="2025-10-08" version="1">MEVDWRYECDAPRYRDFEQKSTGSVNSDWFDTHEEYDSGHIAGGTYADNGILGDISNTVEVHVENVVGDVEAKAMETKKKNIVTSWGEDVKKRKAEDGASAQRSVKTLKRNGVVAASQPGGPALAAKARSKHSKAAGAEGRRESSKERRDLMLVKVSLKRMGKARSTKMPRVFKPILSTRKLTVPDDVELHTDRRAAAHAKDQVVGHHDEARDGDTEDNGEKSPYKPLVLRVKEFDKTPSRFKRKPDDPPPSKPLTITDAKEPQLLTSARVRPSIYKPRDVVEAEEMAYIPTFRASTLNPLILSTSGQLGVPKVDKRGPTEPRPFTFVTDDRAEARAARLAKQQESELGKKGQSEHQTVSDCEGRAKIDRSQAAKMRKSVACHSMELVVPRSPLLRTKFRARESKIEDQRYDFHARPVPKYLSLPSLELPKGFGEAPPALTQPEPFRLATEERGARHRDTLVARLAEEDRAAQRARVPRAHGLPLSTDMPLVPPKPEPRALTVPEPFTLISEIRHVKCEEQKRRLLEEEEARMRTEAEFKARPMWRGIPYRLHDADVPLTVPEPVNLRTSVRAVEREEFNKHIEEKWKETEAAKQVEEEEARLREEEMRRQARKAAVFRAMPMPDLSAPPAVKAVAPVKPLTQPKSPALATKRRRVKKTEEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S716</accession>
    <name>A0ABQ5S716_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="30161" checksum="E5803DDEDCE8958C" modified="2025-10-08" version="1">MSQGVDRSAPPASTSQAAGDVKLPGWKPQQVKKAVQSLLKYVGQQKEKANSLFDDDEVIYMQLALKKMPLQTRKHKPVSLPVPHPLYSPEGQDICLFVKDTPDGQCGKEIKKKLSKLEKNGGVAKVIGTTKLRTKYESYEAKRKLCTSYDLFLADDRILPSLPRLIGKSFFKKKKQPIPIGLRRSNWAAEIKKACAATYMFKSSGTCINIKVGRSSFPPEHVEENVVAALCGAVQHIPNKWTCIQGVFLKTADSVALPLFQTLPDQPQKIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S741</accession>
    <name>A0ABQ5S741_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="754"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="874"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1043"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1082"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1148"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1297"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1465"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1539"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="755"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="883"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1148"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1193"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1542"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1553"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1571"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1582"/>
            <end position="1634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1659" mass="171858" checksum="564EC8BE62A96324" modified="2025-10-08" version="1">MTYLGCIGACFGGFSDCQGTATVKAGLPLHRECSDDNDIPESKSISQATQEVRLRQAESKTQSQTLPSPPSGPLGGPNQQLRPDISLLQSTDTQRTLSSGTDAPGPFLTTGWVAGTKPQSWPTEGAGTPAELVLHTHHLDKVTAKGISVDASEAPGVARASDIDERDPIFDDPDAPGSRSSSPHSPIHSRSDSKCSLDTNPHDDSISSQSMATLATQQMFATAAAGASPTFHFARNSVGGVHACCAGTTAAMAATTVAAGEISVSVEPAFTQFSRHCSSSSLQPMPPVSQVPFPLLTPLLAASSVPSSPRLINQQHLGGQCPRLTVFPSLAAMLAELHDLRLLRPAAAAAAANGTGRDCNIGGYTCGRCVLVKGRLANGAPVVVNLLGCRALHPATGPALRTVMLAGSAVHPAIARVHGMRFVPAAGAQAPGGLTGATRPPGAVGAAAAGLGSAPPPDWGRDEPPTWLPIAGHRREQQNVASGGGCAGEPIPTHLLPSASSATTPMAPAPPASISHAVSACCSATATSARTLNSTATVTSTPLTPAEWPDPAGATGATSVGCSGWLARECHLEQQHALRHYPRQQQKLLFPQSPQLQRPQDRDAAGQQGLSAVVTPCQQLAAVFATLQAQLQLQPGEGILAVLCEGSPMGTLESLLGGAHNPFRPNRSWPAYMARRALLRTALEVAGALAHLHSSGFVHGALRPCNVLLVPSSSDRRNFRAKIFGASVRFAKLLGSRNSAATLTATGDISASLDARSQGHGASSAAAAPGAGRCSSSPQPHISAGLTWPEPVPVPGGQAFAPSYGTLTPVCAAIPTPASVSSTACSPLPSTTSAWWSPFSAAPHSPPSAPGRSRPPSRVLTVAEEDLSFSAAVSAGNDPLDLPATATGIAGSTTGSGSSRRRPSGSVLSPSLMLAMISCPGRAMTSAGGSGGVQPLCRTPSEGQLLAGPSFFRASRRPLRTSYAIPEHLEVEGAETTAEAAVEAEAAMLGHAGEDGTPAAPPADVTPARRSHSDVVLRSAAEASGGNCSGKASLSFTWGGGGTTDGAPAGGPGAPVGSGGGGTSSRLAGLIRALVFNKSTASTAGQTPGLESSPCAGAGSGVGGSSVGGLVRRISNARGGAGTTPSSIRASSVALAAAALLSRRPSWLTANSPVSSSTVQENETSEALWVPDHHQQQQQQGRSHHRRRRDQQEQEEEAEEHQETAEDEDEVEVALFGGGTCAVPPPSPSRFHRGRVPGRGHGTAGAPAVTPSYRPSFEQLPSESLPSVGLDNPSEHAAMHRAAARAAAATAAAATPGTSTAAASKVPTASSTSGGGWRDELFLPPESQQDPLCRVDASHDTWAFGMLLWAMAAGEPPVEGQTAPGTALNCTALCPPIGMLSTLELDLGIRAPAWPASCHTHSHLQSLYEACITKEPGSRPSMATVRAQLEWLERQLREEHVRSRAAATAAALNGFMLLDDQHECHARHNHRHNQQQQQQQRSPKRVLQGQVPFAATGYGGTADVLQQLQQQQQQNQMLPYGRRPLLRIAHGELMDLWGRPRGLPPPSPPHPRPQGVHLPGLLPGGLVSQLHDPTSQPLTYSQHPQQHPLPTLQQLQQQHVLQQRQRQQLGEQQQGQQQEQQQQQQQQQQQQQRGWPVGLSTSLPAPFPYLYARGGLSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7G7</accession>
    <name>A0ABQ5S7G7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_008778</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="130"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI65287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="327" mass="37582" checksum="A6F17D662D28A281" modified="2025-10-08" version="1">MSQPCGTTLTSTNKWLNIFQYCRFLRILGHVMVLLVLALVGFIYTAVVPLTFGPRLISKNIFVILASVIVIIVFTVVTVMCVWSYMATVMLDPGRVPPDWHPFPDEQHARAELERLSYTTYYYDRRDPRRPRFCKRCQVWKPERAHHCSVTGRCVLKMDHWCIWVVNCVGLLNYKAFLLFIFYAMLGCALALLLLLDSMIDFFNNRLRGPSAPLIFVVSIFSFAFTLSLAGFLAMHLQLIAANCTTIEMYEKDRLHPWPYNKGFRRNFEDVFGRNKLRWLLPLYTEAEKRQLLESCLGKVPQAAGLWGPTALPMLSHPATPFLSTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S873</accession>
    <name>A0ABQ5S873_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FK506-binding protein</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009756</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023566">
        <property type="entry name" value="PPIase_Fpr3/Fpr4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811:SF19">
        <property type="entry name" value="39 KDA FK506-BINDING NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001473">
        <property type="entry name" value="FK506-bp_FPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="397"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="99"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="129"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="226"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="328"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI66046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="484" mass="53284" checksum="ED67A6E4B09472F3" modified="2025-10-08" version="1">MAFWSAIVKPGKPHAFVPPPEEWNLHLSQAALAPTVPEGRRVALAVKTDPEDEAIIICTLAAGRGDTAVLDLFFSQYTEFFVIGEHELYLTGYYTPSQLLDGDDEGEDEDEDSEYQPGALGFPVRAGDDEGDDEDEDEDDELDGLGEDEDEDEESMDGDDEEELFGYEAMLRSKRKPHVVIEDVTDHAENNKDRKGAAAASEPAAGDKKAKVSANAGNTVKVEAKEDSEESEEDEEGEEDDEDEGEDDEDDEDEGEEEEEEEEEQDTPAQKGGKAQAPTASGKRPLDAVKPTANGTSKVDGPTHKKHAVGQQQQPQAQPVSKPHDKRQKAEAKQQQDERPARQVLKQQEGATQQEPKQQQKEKETKAGKKNIRSHPNGFTIEDVSYGDPKGKLAKLGQKVAMRYVGRLKANGKEFDRSGKQPFRFRLGVGEVIKGWDFGVEGMRVGDKRRLVIPPQLAYGTTGVKGTIPPNATLEFDVELLEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8J4</accession>
    <name>A0ABQ5S8J4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="105" mass="11028" checksum="FE5C9718257DB850" modified="2025-10-08" version="1" fragment="single">GPGTVRPRRRAVVHLPLPKVAVEALGCMRGGDDARLLTLTSRFGAKLPGFGAEANPDWDPCQQMEWLYQQISHQRAPPPLHPALEAIVAKSQAEAAAAGPGSAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8K2</accession>
    <name>A0ABQ5S8K2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="48767" checksum="BCCE7FA38AA8F6D7" modified="2025-10-08" version="1">MVRTWNREPSPDQDKARREGGPRHTPTLTFQQNNPRFGYDGKIKMANFLNGFDGQLAESRGNMVAANTNTHTNYDFSSTGVGNLSISLAAEMAKSINTCAAIRAGGLKVATARSLVANDTRHDHLEEPHAEHVGPGAYDIERAIPNVGQMPLDSHTSSAIPQRDPYRASAAFSAPERPGSAQWVLPWGPDAVYISPNFVPGPDRAPAWDTNSDKRPDATEWRHSYARVIAAVVAGEGTGGGAGAGAAAPDPPFSSDLPVDAGSRPLDVSLRAQLLNKHGVVFTSKEPRLTALPAERTSDIRSARISALRGLDETGHLGPGAYTPPISVGGSRRRTYRVFVDPAVTPQPSDRFGISGGPGSLLASSQAESAAIVAARDRGTARATAAARVAAAATGSPPGSAAAGGVRVMSARSQAGSCGSLPVTPGSHRGMGGGGIAKLGPSAAFAGTFRTHKTGAFDWRTDSSLVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8W6</accession>
    <name>A0ABQ5S8W6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase III subunit RPC9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009919</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC9 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038846">
        <property type="entry name" value="RPC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561">
        <property type="entry name" value="CALCITONIN GENE-RELATED PEPTIDE-RECEPTOR COMPONENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="130" mass="14021" checksum="BE4D7CB952C7CA71" modified="2025-10-08" version="1">MKLVEVNAGTICNSELFHVLAERGADGTGKATIVERLAYQYISSQPVQPLTPKQLQGFFDVLQPYNLSRGELMQLANLAPRSPVEVHLVIADCDVRLGDEGVEAILAAVAERLAKEEEGADEAAVIAMSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S986</accession>
    <name>A0ABQ5S986_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="34456" checksum="BFBF6A95DCD6E2A0" modified="2025-10-08" version="1">MDAGTPPLPVTPAYFASCCISGGAIAPAPIANAHLAQAVETDASISDASGCFPSSSFITSFVGNTPFPTVAPKSGQPVGGHVKQKYRLVSPLFWLIPPVAAFSSEGGLLCTDSVHALALARAAAGRRRMMAALEEARRRFSHLSRSKLPNSWPSTAPSISTAAQELQFQREGSSREGATGSRCLDEGSEMQRPMVAPPARLLPNLPLAPRDVGILSVGYAASIAVVPCEASKSVSKVPETQQGRAGEYGRGNQEGQYEMGGRDGGDCDGRQCANAATISYLQSYRILGTAKLSCESGCACEATTLNAYDPRPVSVAIPSDIKADLKLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9K8</accession>
    <name>A0ABQ5S9K8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010357</name>
        <name evidence="3" type="ORF">VaNZ11_010413</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="2 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000034">
        <property type="protein sequence ID" value="GLI66541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000034">
        <property type="protein sequence ID" value="GLI66549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="151" mass="16120" checksum="1DD7B8960250E813" modified="2025-10-08" version="1" fragment="single">QVSDLISQLEKLRSGAAMMEARKGGLSGASTDLQGSSITAKFAIIPAASCPAMGATSPPSAVEVADAGAASIFINKAAQQPQELYQPLELTQPPKPHPKTPERRLPPEARNWGLQDCRSWAGEQHWSSWAEVQAFTKTLEPTLAEAIREAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9L0</accession>
    <name>A0ABQ5S9L0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Luc7-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010541</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="246"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="94"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="246"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="270"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="401"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="48189" checksum="B0856B7429902C8B" modified="2025-10-08" version="1">MVDAMRAMLDELMGKERNVPLGQRSNRRIRFDDPTVCKYALAGLCPNGLFKNTRSDLGPCPYELHEDHIGWPELQAEYDKQDPREHERYERRLMKVLEDLIREMDRKIAKSKERAEAESSARPLKPDDATRLAEMQAKAKELLHKSQEAGEAGDVDVSMALAQQAQEMQAQHDRLHRNLTAPERTMSVCDICGVFINSTDNDQRRQVRSSSQPTPSMLAAQEHLTGKQYLGWKAIREKYAELQNKFERSRSREPEPTVRERSAPPQVGEEPSHRERERSPVRREHVSRERGSYDDRGGYESRGRSAGYDDRRNDSGREVDSRRGDRGYDSRGRGYDDGRSRAYEDGRSRCNDDYRGRGHDGSRGRGYEDGGRGRSYDDRREYDRRGDDGYRSGSYASGSSYRDRPRDGYFEERRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAE6</accession>
    <name>A0ABQ5SAE6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010927</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000047">
        <property type="protein sequence ID" value="GLI66900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF23">
        <property type="entry name" value="SMP-LTD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="532"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="899"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1080"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="390"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="539"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="901"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1089"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1119"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1172"/>
            <end position="1181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1213" mass="128232" checksum="497987F0F16F9978" modified="2025-10-08" version="1">MLRVTEEVLAWLASFACLIYILRSIKQVLLPAGGLWRSPFVMFTAGALFAIIATAVASACCVMILTERCRPKSDSHQGAIRGGNAPVRRPWLLRRAKGHKGEGGAVNLVTAHPSAVRQGSYTGPVWLVPATLWTRGQLASQWPPLTQGPAAIARKFHATLVRSRTQYAAALLLVPELVARGGGSTGGSQQALLTDIDHGNYPVLSLDDVTVDLMRDILGTRSPWTKRCPLQLSHPSRPLLMGETTVYMFAESGSAKEQWYAALMSAVAPQGLLATTQHMYEDYCRYMASLQIVQPPPLPPPSPPPAMLQPWRFRTRNMQHGRQHRHKEQRQQQEEEQRHPHHQEQLQQQQRSDATSAPTASGAPAATTAVKGSQPHAPTGKERDEKKVNQQRQHQQGQHPLQATGGPSKPSAQLDPVATAPQRGGRNPFGFILSRFGRSACRRSDNPVATPAVAVVATTPEAPFSNLPSHVGSQTSIHPSEAAQQPLQQGLLGRMSEQQQGQQEVLQGRLEGNLTVDPGQLTGMEHDGAGCQGWEQAFSRPTGTTDSNASTSACQPLPRLPSSPQQLPLPSQQQNKMDHQEQGEVTPLEALDVSVAPDFSASSARRAVSDIFTASRPFSQSCQVTPGDAMKVPSASAAASTPVKQGRDFAVPDGPTAGGIALLRAQPTLSEAAGRHDEAASSTRTVAARKGIAAATAGDMSLSVHSRASHAYASTSAGMGAVTAGGLVHIPRSGHTEPSFNSWSQLQVAGIGMATGLVADVVADADSPMVVPAEHPRAVTPSAAPSSSSANTAAVTASAPVLVPSAPDAGVLAAGVPGITSRTESAALQAATAEAARGESAGVEAVAMAASDVLEKQLQTAAAPSQGKQQRTPLGTWGCAQDNLEPLVSSRLALKAPCSGLETSGSSPCDRSPTASGSSTMALQSSHILAVAAAAVPSRTGVSMSLPLLEQQPRNSGSMPDLLEVARWAVEKQDLGGEVSEDSPFSMKAAAGESTAKTALSWRSLDHCLGEGSSIPKGHGPMGFKQGAQQPILRDEARHAISLRVPSPTETLAPETNTAGALLSGRSCNGAGSVSQRVVAKGGMPKNPSKHDLKRMQREEQERLKQEHQRAKAAARATEGARRAEERAARERARSEAHYRRLEDEARARQAKERARALKQKLTSAKSVQDLATAAATSATSEPSLAGMHRRTYVQRRNSTDGGRWFVSYPSGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAU5</accession>
    <name>A0ABQ5SAU5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Abnormal spindle-like microcephaly-associated protein ASH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_011252</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052614">
        <property type="entry name" value="CFAP65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053879">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056344">
        <property type="entry name" value="Ig_CFAP65-like_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056305">
        <property type="entry name" value="Ig_CFAP65_10th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058536">
        <property type="entry name" value="Ig_CFAP65_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057470">
        <property type="entry name" value="Ig_CFAP65_7th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46127">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46127:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22544">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24291">
        <property type="entry name" value="Ig_CFAP65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24507">
        <property type="entry name" value="Ig_CFAP65_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25249">
        <property type="entry name" value="Ig_CFAP65_7th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24816">
        <property type="entry name" value="Ig_CFAP65__9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24771">
        <property type="entry name" value="Ig_CFAP74_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="8">
        <location>
            <begin position="226"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP65 fourth Ig-like" evidence="10">
        <location>
            <begin position="340"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="8">
        <location>
            <begin position="672"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP65 seventh Ig-like" evidence="12">
        <location>
            <begin position="781"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP65-like ninth Ig-like" evidence="11">
        <location>
            <begin position="1071"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP65 tenth Ig-like" evidence="9">
        <location>
            <begin position="1269"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1021"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1728"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1763"/>
            <end position="1791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1803"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1904"/>
            <end position="1951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2041"/>
            <end position="2081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1021"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1823"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1904"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1921"/>
            <end position="1936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1937"/>
            <end position="1951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="2053"/>
            <end position="2071"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI67062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2142" mass="225442" checksum="1E24B664BFDA2DA0" modified="2025-10-08" version="1">MPATGLDQQSSATLAMAYGVLTREQRKQMFGLDCPEQLEWRHWNPGTEYIKQLVIKNVSTSVLKMRYKQPASKAFSMDFPEPFKLRPGMSQPLKVTFRPLKMQHYSDNIELFVGSASFLVPVHAYTPVTHIEVPPSLDFGFTPTKETATAPLVVRNSGDVRIDVAWRLEAPFVISPPAANLAPGEALSFTVAFTPPEACSYTANAACELESGAAAVCKISGIGKFPYLSVEQAGVDFGPVVVGQKAERVVRFGNHSMVPANFTVTHDPDGPDDGVFTLGPSRGTLGPEEYSVLRVSYTPRSTGTFSSETFQIGTAGGNVVSLNLRGTAVAPTITLSARAFNFGNITIGASTSRVLYIRNHSAVPVPYDFQVEPLDVFAISRVRGVLAPEATAHVTITFRATVPSNYWRRLALMLKDADPQGIDLVATAYSDKSRPPPLYHRHVDRYLTRVIAGGLPIDEAHLASAPPSSDMDSVISPKGALAAAAVVSPSAADITAALVGDGTAAASADEDGTSAARLVGPDGWPLIFFGQDPGGAVVVDTELLDFGSCSRLSAAEYRSVTVTNTTPAKLTAFILTPDWIDPGAAPGEPPRPVFQVFPESVDVRPRGQTTLKVAFRPPRDGAFFSQQLLLVAHVKSQRNFRLVSEHQMLPSWCLELRALGNTFLHSNPEFSPKVELSTRHISFPPCRPGEAVHQTLLVANSGDTPSSFNFGPPGALGPLFAVRPMTGVVPPKGHVLVGLRFHPTDTRPAAATALVTFNGVASNGVSLSLRGCAHIPRLATDLPSGTLYFRPTCVGASSQRAITLHNPSRVSLDFRWQLPTKLAGIVSVTPVAGTLRGNESVQLLWSFAPAAQKVFEGKATCIVLAPDGGSGGGAATAVTATDAATSETDGVLLSLVGEGTQGAVALDPPVLELGDLRVGHPVRRPLVLQNVSDGVLRYSLEVVPAEDDEPQAPSSNACDFTAGLDAGATAAATAAGLECWVDEPEGALPARAAKTVTVTLFPRFRKRYSLQIRCRSSTVSPLLTGPNNSRPPSAGRGGLSPVGADAAAAAADAAATAPPVIAPLTAAVTFPTIEVTDVFSECQPKQLLWQMLGLNDLNYHLRTEVTATEVKLRAAHDRGSLTTDAACAAMRPFLMDFGTYGLGGDVRVVHLELSNPTPLPVSWQLHSFDDPDGVELENWVEPGRPRNEEERVRDLISEHRLFEMRPRSGSLEPGARGTLTVEFRPRVEGVFSLPVFLHVTDGKRLRLQLEARTTAEARQLLALPPQLRTFRLEPVALGDPAPPLQMYTLRCGGPAPLRWRLDTAPLDVLAKESWGHRVLDLVGPAEGEIPQGGVASVNWRFCPLEAKEYSMRVPILLGDGGVEVIELIGRGYKPPPTPHHGALRAVAVADGAATTDIGLAADAAAMETASAATADRDWATWQGLSDAPSAGMAGRLAFVDHDLVSMGVTPLLGLTRRIVVLTNKSRYPLAFTWDLAALAPPPGVAGPPLPSSDVLTGRPLQAALAITPAAGSLDPGERLVCRIALHAGVTPQLFEGEVKCSIRVDEEAMEEAAAAAGAAAATAAAGDAALVERVEEVIAEDPAPLGPPPLPPALAAAQRASKLRSRLPVHAYMTTAIRNRIEPLAQQFAATLETRTRRATEAARGVVTIPEPQTICVTLTGRILDNNQIMALRYVPPHERHLARAAVTEGACWVPPPVVPFWEMVLPPPGGAMAAALPLSWLPAPLTDTAAASPPPPLPQQQRPSSAVISAGPSAADFAAVTPGGVGDGAWPSLNPDPDRPGSGSINGHVPDLSLNRARLQRSEAGQATPVLGPIRIGRSESIPPPLPPLHPPALAAAPASAAAAKDGISPSGNYVADVTFATPLAAPASPSKSSLPEADGSVSTVAAAAAVADSELDADSSAIASSRSDAAGSVGGGGASSTITGRSSSMTTPTPRAEPVPPPLPPPPPLGGGIEYDALVSARDVLSDLFSELLDDPDLRRAFEFLPEEEMPVFAEVRSRVAPCYDPQLRAEVAAAAVATEAASDAAATPSYTAVTAGVPPIQLGHHNSEGQQQWNQQQRPLQEQQELAAGGDGGGGTATCLTLQEELAADPELRRVMMSAEWRTLADFVLESALLGLIQESAAGDWDLPTESGAADGRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAV0</accession>
    <name>A0ABQ5SAV0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010926</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000047">
        <property type="protein sequence ID" value="GLI66899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF0">
        <property type="entry name" value="SMP-LTD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="532"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="899"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1080"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1756"/>
            <end position="1777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="390"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="539"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="901"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1089"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1119"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1756"/>
            <end position="1765"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1777" mass="186621" checksum="94356F238690A457" modified="2025-10-08" version="1" fragment="single">MLRVTEEVLAWLASFACLIYILRSIKQVLLPAGGLWRSPFVMFTAGALFAIIATAVASACCVMILTERCRPKSDSHQGAIRGGNAPVRRPWLLRRAKGHKGEGGAVNLVTAHPSAVRQGSYTGPVWLVPATLWTRGQLASQWPPLTQGPAAIARKFHATLVRSRTQYAAALLLVPELVARGGGSTGGSQQALLTDIDHGNYPVLSLDDVTVDLMRDILGTRSPWTKRCPLQLSHPSRPLLMGETTVYMFAESGSAKEQWYAALMSAVAPQGLLATTQHMYEDYCRYMASLQIVQPPPLPPPSPPPAMLQPWRFRTRNMQHGRQHRHKEQRQQQEEEQRHPHHQEQLQQQQRSDATSAPTASGAPAATTAVKGSQPHAPTGKERDEKKVNQQRQHQQGQHPLQATGGPSKPSAQLDPVATAPQRGGRNPFGFILSRFGRSACRRSDNPVATPAVAVVATTPEAPFSNLPSHVGSQTSIHPSEAAQQPLQQGLLGRMSEQQQGQQEVLQGRLEGNLTVDPGQLTGMEHDGAGCQGWEQAFSRPTGTTDSNASTSACQPLPRLPSSPQQLPLPSQQQNKMDHQEQGEVTPLEALDVSVAPDFSASSARRAVSDIFTASRPFSQSCQVTPGDAMKVPSASAAASTPVKQGRDFAVPDGPTAGGIALLRAQPTLSEAAGRHDEAASSTRTVAARKGIAAATAGDMSLSVHSRASHAYASTSAGMGAVTAGGLVHIPRSGHTEPSFNSWSQLQVAGIGMATGLVADVVADADSPMVVPAEHPRAVTPSAAPSSSSANTAAVTASAPVLVPSAPDAGVLAAGVPGITSRTESAALQAATAEAARGESAGVEAVAMAASDVLEKQLQTAAAPSQGKQQRTPLGTWGCAQDNLEPLVSSRLALKAPCSGLETSGSSPCDRSPTASGSSTMALQSSHILAVAAAAVPSRTGVSMSLPLLEQQPRNSGSMPDLLEVARWAVEKQDLGGEVSEDSPFSMKAAAGESTAKTALSWRSLDHCLGEGSSIPKGHGPMGFKQGAQQPILRDEARHAISLRVPSPTETLAPETNTAGALLSGRSCNGAGSVSQRVVAKGGMPKNPSKHDLKRMQREEQERLKQEHQRAKAAARATEGARRAEERAARERARSEAHYRRLEDEARARQAKERARALKQKLTSAKSVQDLATAAATSATSEPSLASAGAMPFATVTVPHRSSSGGGIAIPSCTDNAMARESTPVAQAPGVVKSATGDGVEAEPEGVHCSGQEFAEPSVHAPLPAPPAPICGALVDSLYGLNMVLVRIGFELLRRRGFSGWLHRLIQSKLERVRRPGFLDKLSLRRFDLGFTAPQLRNIRAVAGPNNCAPDASTWPQLALDLIWLGNATAIVSTKIDFDRMASNQVDQPAATGSTSEPVARMPTTPHMAAGTISGMTFGKAAAPKAKSVPIAPPFLVAVPVAVPVPAEAAGLSCRRTADEMADEVADVTTPAAVTLGAVPLKEYIPSLENHAGAITDTVIGTSLPAGTRSCAASSGRNKAGGESGPALMPVLPAQPQPALVVEAQTPSVHLGAKQPPDAARTESWAGRGRVAGRSGGGGFWMNFVPFFALLRKAGQALASNLAGSLSGTPVEVHLQARRFECTLLLWLAPPPSDRLWLSFAEPPKMDFTVTPVLGNRKLSGGLVVGRLSAWVRSKVTDGLSRAMVYPACVDVRMGKLLLSIDSPGDAVRPFMPPPTSAPLQTFAPISELPASPSRQHLRHVSIGGNQHLHTLSASELGASDQQPPAATGTGAQLPFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB41</accession>
    <name>A0ABQ5SB41_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyrosine-protein kinase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011321</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF289">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE VIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045122222" description="Tyrosine-protein kinase catalytic domain-containing protein" evidence="2">
        <location>
            <begin position="44"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein kinase catalytic" evidence="3">
        <location>
            <begin position="586"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="712"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1036"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1392"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1732"/>
            <end position="1752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1771"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="731"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="775"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="800"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="822"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1036"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1049"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1074"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1738"/>
            <end position="1747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1805" mass="182692" checksum="4BBD5E728720367E" modified="2025-10-08" version="1">MNRFTAGCGSHLQDRLRSPSKRGNTRLLLLALAVTCLLITCDSHQNATAADFLDALARSQSSLPGSASDIFPASIQTVTPGIAADLRALHYDPARSPAAVIKTSAKLTGAVALLHHQGSDHLDGKREDRVLVDLGSASRGLLFLNQGVELILEHITLAGFEQGLTQIAKEPTQIADGLGGKNGAKDGSANIRDDASVPKHVATLISERLIHRASRGTLMLNDVILALGHCSNVEWDIGAKLTQALTDRGVRGHPDLQPQVEMSFGADAHGNPASQLPQQEDEVDVEIGESRVDAYRHLLRGPGQAATITSATITTTQRRTEQNAEWKQQSRGQANAVQSTAAAPASESGFRRTGHLGPLSGRFLTKGGAVTELRNTFVWCTDDFAADASTNDASLVDDIATAAIAADAAAGEHRSGARKTEPGRRRLTATVGFSQVLSVVQPKSVSAIPSMVQGLILVGVMMVAVGGVWLRRLLSCACLAPSRQAPTTVSDASRTQNGSRSFHKPSTSLMCNSHNHALSGLSRFSAQLRKGEDSSVRDGSIRRGSILSRIGVDISVRGGGGSVDGRGGGNRNQPCLPELPALVNELSEIKPAGQGRLAITLQGIWQHQQRVVVKVLTHAEDCCFMLEQVGEVWTRLTHANIVTCVAAETFPASALASRPAMAELIPQPTSTDATLQTWLVLERCENGSLAAVLAQRYQQQQQQAVRKAVAAAASTGATEPPPKEAATARQGNRRQGHSTKGKRKSSTDEVAAAPATPSQDQGLAAPSAPPALEVPSPSAMAAPPLSLPSPKTVQSGEITAAAASGPSSCASGTDGAGEEGDGGNGSGGDAGNGGGGGGGSGDTQVAPLPGLLPMGEVLSIAHDVASGLAYLHSLDLCHGDLRAENVMVQTIVLPSGTRASGGESPAIDLSATAPESAVGGGLCLATVDLAGLPYVDGTASSTAPVASAAAVALGSCHTVDEGLAVMYGSTGGHSDVTDGPGGGGVSCAGGAAVNIQRRMASNISSCSNTANASCTGVPDSSGHGWSNSLRQSNGFMTGSTYTSTMTTQAGGGGPGGGGGGGRSLEPGSVDDRLGPGSLQSQGLGSNSYFSHVGRPMGQPYPLSGYNKPSTGGCHSAMGKYGNVAAAGAGAAEDGATTAASDGAALASTALATSAVVALSRAMGVGPCGFSQQQPQQVRAPALMAAGGASFARRQSNSSLSAANTNSGNDVTAMSKALSGLKAWPESPHEGRHAGGPAAVATTSLTTAGGSSGGAPVTYNRMGSGAGGALPSPARKTPLIGGLKSALLAPDAAKTMASESASASASALASASSVAVAPPMTPQPQALAPNMSAEVSPFTRVLCQAPFPDDDSSLASPVAPARLPSCPSDGPGVCSSAVPVVSPRVLALQINEAPDSAQPPSPSGGCGGDGCSAGPSTPTGGGSGGSGGGIVAPAAAGGSGEAGAAAGRSRNAVGVGVVCTIAAHQGRRSSGPSAAPPALPVIRRIAKLTDPWLSLARMPPDCAGGPWTPGAAEAMGVTSGADVSHLPPELLLSGRLHPSSDVYSLGILMWRMLSATGEAPYAKLRPAEVAYKVVVCGMRPSFSPAVPEVLRRLVEDCWLSDPAARPTVQQVIQRVSELQANAVVLQNQWRERHALFGLVSTVPISAVAVLPLQGEQNEVAARWADAAGIKLLRNSTSLGGASATAAAAAAIAAAAGRRELSGHASFGGRSSRGHSGTFNSVTSYPSIGVSGSAAHLYQQPPPPTPPAGPITNMNAMLFSTTTMESINYSNTMSEGHVRGSHSNHRPTSPFIGHLPVQQDRRGLDPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBS4</accession>
    <name>A0ABQ5SBS4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GLTSCR protein conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000045">
        <property type="protein sequence ID" value="GLI66889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="923"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="815"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="923"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="937"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1074"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1093"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1123"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1144"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1169"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1183"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1197"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1213"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1259"/>
            <end position="1269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1269" mass="129652" checksum="A3AE1F1B1C613474" modified="2025-10-08" version="1">MSKPSTPVRRSKVGPGRPGSAAGGEIPLTASMEVLTPDPCVAPHMAASELGRSQELHVAPVGSKPRTPSSATLIRTRSTDGRRVSGPHDVIPLNSTTSKSFSANQILAHPLVLATQAGSSPACSVVGTPGVILPPPQSPYLGGPAPGGMTPSGAGISGSHATLPPGARLRTPQDIMASTVSSRFNPVTEGDDDMPPMRESQVVLDGSATAVMQRVAAAAAVAGTSSTRRYAAEESVALSQQQQWPQTGKVGREEDVGGSCGGKVLRLQEESEVPGTPGSVASGRVGGVFFTDLHRSFGGNARRSGVSDSQDLRGALAADEEWPVEGNDDESDGLVSDATPHTRISTIISKLNPMQSPTTSEDGDDGDEDSFAEYAGEGLRSGREAGSGMAEVAGDRRTSTSALTDYTNPVQTPYDSRLPTPLLPSDGSKEPASAGPPGASLETAPAASLLLVIHDLYVTDSEPRTPADTSRPTTAASEPPAQTPVIPEQQPQPAPEEGGEGDEVLMDESAALSASRAAVAALAAGTEMEQSQRRSELGEYMTSLQELKDESGGRLVELPADWGVMLERLFRQRRAQFETLQGWMGPRRFEDLPVNQLPSNHPDPRVEEGLARIRELDAVLNDKLIAALISHRETFPDQWAEQERKRLERHTKTVEEALKRERQKRLRAAQISRAVTSLDGLEVQAASPLAQGSSRSLAAQPSSVYSRLFTLKPEEEKLVEAVLARNDDEGAAINPFDLESAATYGNMPDGGSATGVADDDGEAVADPDKIDLELDMMLPSASASACTTPSAAAPLPRPLSARLTEADSGAGAGLRPLSASSYSSSRPGTSSGLAGGMSPLAAAALHGHPALLAAAFAGAAAGTRASAAGDGGGGLLPERLYDDPRAAARALRAINARLAAFESEHLWDERGALADFKSISSAQQKGANQKQQPETEASVGPGSAGAAVGVQSSHGGQRAASPPRSLRSVAGGPAASVTGASVTKTGQRDYLREERLAKELATRERDVDAALRSLKTGEVVRLGEATLKALVEQCRRLQELNEHRRKIEEAEAAARRAQLMRQQLMASRASESGSGKGTPKGGHAPGTTGSGCRGQAPQPAPADQARGAGPATDAGNGRGTKPATAAAATSASTAAGSGGGGTTKRQSSAGKTRSGDSLPSAGSGPTAPTPSSRRASDPASAAPGGGSAAAGGGNGTGPATTNSGSGIPKSGKAPSAPAQTASVASTSASAASVGAAVPKPRSAGKSGGSPLQPDGRGLLPPPPPPPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBV7</accession>
    <name>A0ABQ5SBV7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein CASP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_011528</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CASP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012955">
        <property type="entry name" value="CASP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057476">
        <property type="entry name" value="Cux_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043">
        <property type="entry name" value="CCAAT DISPLACEMENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08172">
        <property type="entry name" value="CASP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25398">
        <property type="entry name" value="CUX1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="655"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="Cux N-terminal" evidence="13">
        <location>
            <begin position="12"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="CASP C-terminal" evidence="12">
        <location>
            <begin position="464"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="25"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="123"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="237"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="323"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="530"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI67351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="719" mass="78518" checksum="45647ED48D50E739" modified="2025-10-08" version="1">MTSDNQDAIVSPLSVVCNFWREFDLERIRTKLDEIGLKVAEHQEESMQNRRKLAETTREFKRNNEAVSRTVGTLLKQYQEEIDRLTKRAKHGETAFLDLYQKLYEAPDPAPALAMAFENASRATDLEAQCKKMATELAEYKAEAAQIRNQDLTVRKLEEKVRALEAHSEERDKELQEVRQRAVAEADAARLAAMQSREEELEAMLSQAQASLLAMQKLYTGAENQLFAIQSQSEEERMGRQAELELASAELERAQERLAALEREKETLVAKLQHDAAFALGGSSGFGGTAAAAAASIEETLRTELGVQRELAFRLRHELAAVRAESEEVREMLEARLEGLKSTLAATEQHAAALEAELAARPTPLQLEELRAQLRVLQAIGYNTLDVDDDATTAAITAVSTAAGANAGGGAGVGASGRGSGLGAVGSLEAMLLAKNRHLEHELTMAKLRVVDVRQEADAALVHAAELEAQLAEQVALVRQLEEDLLASRAAEARLAAGAGAPGGSASSPNVAGLVASASGDTDDGDASMVRVLCAQRDRFRARVHDLEEELTRLRAELAATKSAAAAAKADNLALIERLRYVHSRTTPAGDGKAGKGADVEAGAEVERRYGKIYDEGINPFREFKEQQKERQKSAMGLVDRAMYMLSQLVYGNSAARMFAFMYLALMHALVFASLVRMTHHSSHQLYSHTQAVLQDRHDATAAMQHELIHGNPMQSRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBX7</accession>
    <name>A0ABQ5SBX7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="662"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="585"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="662"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="712"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="726"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="736"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="756"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="777"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="787"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="798"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="833"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="845"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="858"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="991" mass="100171" checksum="9D70666A843D156D" modified="2025-10-08" version="1">MMEGRPRSAVRTASAVAVVSASTEFLERGAMVGAGQYGVTKKASFGANGLSPLMLPQQPAALSPVTSPALPTPGPPTATAADLHAMVIALDESRDFHSVSAAPSPGPTGPPSPSPLSSHSPQNCTPPSPGPLASSSPTLDLEPTPGSLKLFCSGTVGEIGRSPSPGPGTRSGSRASLTLIPKGVRANSPSMNTPTARHAAGASPLSIDVDATVTAAAHTGASSPLRQQGWLESVYSPKSPSTGCSGPGSGSPLGAGPGIIAPDASPAVRSPMGSPGRQRGTGAPGAVTPASSSSPGRAGVSRGAISLQSVYSPTPPPPPVMVVASADADWSHTGGRSDGGNGATVAKDISAKGNAVTIDPLPVKEGLRAYYLLSPASPSAVARPAMSSPVRGSNPGSSPERSGSPRRAGPVSATATTLPQKFPSMSAAHQAATSSPGRQPSTAPIAPASRADVASNASMSNGSLATYTLSAENVRQPPAPAPSRADGGYNERPAAPAADLGQGLVPGPGPGSAALAAGRVSPADGRVSTAWSGPGRSPYGAVSAAAQGPGYFRGQASASGGSPAGSPPPPPPPQQQQQQQQQQVSDRRAARREELFEEQDPAVVLEQSIALLSEIEGHIRESVGLHADHPLQSIDPDLAQDIPLQLVEALEDTLEVIAMAREELAKNRVGDPGRDRSRDMERDRDRDRDRGPRPPAYGSGTPLSLYAQYRSIGGGSSAGGARGRVSRTGSQPGNGLSSGGGGGGSSGTSRPPRGSWAGGGGSSSFGGAGPTHGGSPGSHLPPISGGSSSGGGAGSGRSGASLYGRPPSQQHSSRPSSRQHHPQPHSQPPQPPRSSSISRRTSGSLSGGGGAGAGGGSMTSIPSLAATVSSRRAWHREPPPEEWVAAKQARADSERRNREAQLAADLRYLQQIRGRASEVQQTHEALLAQAEESVRAARSAKASTEAQHRWLQYQETVAEGAKRHYENLMTQQQGAPNVHDGACGPTRYRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCP1</accession>
    <name>A0ABQ5SCP1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BUB1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011435</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015661">
        <property type="entry name" value="Bub1/Mad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013212">
        <property type="entry name" value="Mad3/Bub1_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030:SF4">
        <property type="entry name" value="BUB1 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030">
        <property type="entry name" value="MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08311">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00777">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51489">
        <property type="entry name" value="BUB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BUB1 N-terminal" evidence="2">
        <location>
            <begin position="40"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="720"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="840"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="671"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="683"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="754"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1057" mass="112699" checksum="80DD95D8B6626E33" modified="2025-10-08" version="1">MDWELTKENCVPVRQGRSKAALEEMADPALDMLEAKRRELWSAITEYKGDDPLEPWQRFIKWMQEYGVGGGKADLMKVLEACTRELRKYPRYTNDIRFLRIWIQYADCLPDPDDVFMFLKEKGIGRDFALYYEAYATHFELKGNFQAADAVYLDGIQRGVKQLERLKQKHTAFQQRMAQRIQRRIRDEHVMGPPPEEPVRASLVSIRPRGSGRGHQGQQQQQQNAVGIFGVAGAPQQPVGLLGLQPNPLQQERGRRPIIRLREDDEFAGSENPSPGPLGYSVMPQVDNVRELQPYGVRRKENLDRALAWSHAPLFPGVATTAAGPPVDAPIGVEIFTDEEFQDGDDGLPAKAGRSGQVQHGCPSASASQSLDQSLMLPETKRSAPGTVSGGADALILSLAPQRTLLPRPMQSAAESFASLSLSGSSAAVPQHAPLAALSALPGRLTQAPDGAAFSRASHVITPGAMPVGAGAPAPALAAELEEAECVQQSGNGEEEMSYEERRAVVWFRRNPASRHNALPVASVGKPAVVGSISMDHGAQLAAAEHRDDGLPNQRTVLLNGSNDAENGDGTGVSYNTGNADVVGTAPRCAPFGPVIPTVETTAGTRLQTLQGASSSVQAHESRQGAPNGHQQRRPLADAMPVARAPLDPPASSLTCPGPPTVRPVGIQEEPQPQSASCVNTRARGDDSNHDDAQPTVTISTRGAFDLINSLFSEDLPHQQLRADRGPPRPSNVLAPQPPSLAPVDAIRPPCPQPQVAQSSPSSAPKQPPATEPTVTLHTKQAFDALNDMFCDTLPHEEARKLARRGTAASASNSGFRNGATYLKPISTSDVCRLANAARSSTAGAQGAGPRGPPCGEAGDGSYGRAFNGSFEPNPVMARPFAEAAPGLLIHEDTFLGPAQPFFSGGTLGGSAGVGARAASGQGHAQDVAKHPEPAAIYEDTEFIPRGGDLSTGRSGCAVPPEPGFILEDTEFVTRHHGPHMAASNIVAFDRGTTNAHGGSRSGLASRPVLGGRQGARDLCAMIADSKNTASSFESRYGLRKDISLQIYEDTQYNLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCR4</accession>
    <name>A0ABQ5SCR4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011981</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="224" mass="23546" checksum="B62DFD48F084F8B0" modified="2025-10-08" version="1">MRRRAPSRNPPEEAVVAPAALAAAGTIGGKASDREGPEVMVREDVAREGEEISQELAAMGSRRRCEAVDRQGAERLVAALLHLKVARGSSPGGMEREGIGCCCSCCCCSDDDDDCGVDREGLVATENGLGSLSSGKSEVARRRLSTRSATTVASTCFGVSSTTSRLLFAWKRSSGQDTTNCTCARARGRPSSSPTALIATSVPSYKICLPCRDSPVASCWPKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCU3</accession>
    <name>A0ABQ5SCU3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011846</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF376">
        <property type="entry name" value="EXOSTOSIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045513859" description="EGF-like domain-containing protein" evidence="7">
        <location>
            <begin position="23"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="742" mass="83051" checksum="B3EFE1776D6F2354" modified="2025-10-08" version="1">MGAKSWSLLLAVALGVTATVGSQSKLQYAAGSLTRPHLPHRTSLHESSNPGENLPNLANLRNRGSLQTTTRAKPRCETSQHPFVTTSLCEHQRRTRSLKWSAAAGSRTADSRVIDAAVADDDSPMEPPVALLAQVPDPPPQLVASPPPGPPGRRPCSEPCYLRATCNEELGRCDCPKQLTGSECQGNTSASYLRYFTQTAKQEAAQPLPCLNRCNARGKCVLGECVCEKGFFSADCSLSIGPSGKPVILEGKGYLPRDQRPYIYVYDIPHRYSSWYNPRRMDREAHWTLWQQMLPARVLVADGDEADWFFIPVKLRNSGDGPKLFKVIKYIQQHWPWYDRLQGHRHFVIHMGDSGRGEVSEDVRQAFINMTWLHHWGLYEDFPYSGWKAAHRPGKDVVIPVAFKTKVVKDLLSYSPLHPRAQHLHRDRGLLFAGRICGDYSPPDTSKSWPHCKTNKSIGYSQGARQMVHFYHHNRTGYKVVTADHTYLLDIMRYRWCLAPSGGGHGHRQTLTAAMGCLPVVVSDQVMQPFEPELDWSAFSVRVALSDVPGMHNKLDAVGDIAYDRMQAAVQCAAQHLLYSSSNGAVMQEDGRWDAFETVMEILRMQQLYPGLDPGEYGNRDERFRLFMGCGHEHMADHGYAQRQAWKDRYPVPLEHVYSNQRSFGWDVMYEGGLAALIKRAGTKSEGLVRTGGAQGGDLEREGVQERGGGLQLCSVSRWDVTTDRCEIRCMKAVANGGCRSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDF7</accession>
    <name>A0ABQ5SDF7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotide-diphospho-sugar transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053250">
        <property type="entry name" value="Glycosyltransferase_77"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46936">
        <property type="entry name" value="ARABINOSYLTRANSFERASE XEG113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46936:SF1">
        <property type="entry name" value="ARABINOSYLTRANSFERASE XEG113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="3">
        <location>
            <begin position="517"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1019"/>
            <end position="1029"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1029" mass="111957" checksum="26B322F4E067C551" modified="2025-10-08" version="1">MDHRRKHTAHRYLAEIDHEIGDHAKGSSGHTPASHRRSRLSTVALLSLGLVVLFVGIVTLFHFAHVNKLEPMPIKAADKKSVVLRRPDGEGRPDSRAVGTAAADGSHIEGGLGDDDDDGSGNDEPVDKGENDDDGAANDEPVDKGENDDDGSANDEPVDKGENDDDGAANDEPVDKGENDDDGSGNDEPVDKGENDDDGAANDEPVDKGGKDDDGAANDEPVDKGGKDDDGAANDEPVDKGENDDDGSGNDEPVDKGENDDDGAANDEPVDKGDKDEEEADGDTLSGATAGDGDNDDDGTQNDAPGNLDEDDDTEEEARNATTAAIGGGGSGKAGNAAAKSDSSGSGATSVVVGTAPDSDIAHEVDSADPEAVDGITDYDDDGWDDLYVDAESGTEEADYEENQDLELDPVQKLLRDKHQHQHQPEDKHKQDEGQNQGKQQQEKAQKQESNSTTSAAAPNLTADAVKAADVGCGSLSRQLLSKWALNNTVLLTFTNSIMFRNFGATWLYHVRKAGIKYWVLAVADNETATMVRSAGADHCFLVHENEIDDTAAAFKWGSRSWQLHTWQKVLTVRHVHHLGFNVINSDLDVVWLRNPLEHFLVKYPAPDYWVSMDPITTANPLGDDGPEVGVSTHHYMNTGVYFLRQTPGGRALIDKWYDIRGQMQQSGYHDQDGLYQYLKDSGQDVDHVTRVTKVVDRKTKLAQLPATLFQNGYSHCINQIHKVHGLKPFEVHFVWVWSGNSGKINRMREQMYYIDPPSYYSDGYFLSFDLEPIATPPGFNDWTDTEAMVQTHLTSLDSQLTDTWHGLALAALLERIAILPKMKCFCIQNWFENPQCRLPGEPHTRFPLSPACPADYVFNIDALKTLNLSGRHVGFREFSFLDNERTPAEVKDRPLVVVASAGATTVSRNGDTLTIPAGLKSGPLLAALQPFLKLQTAVNSKESRKEKPPKRLHFRNPRLAFGGWSDPQVGKEYELHVETLPVRWCCRPDAVAKQHGVEVHHQLRMHQALPGAGGAASGGNASSRGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDP3</accession>
    <name>A0ABQ5SDP3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012041</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="226"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="531"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="254"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="777"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="779"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="807"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1364" mass="152727" checksum="6748621B4E1119EE" modified="2025-10-08" version="1">MGKDEDTTKVFFGRDDYFTVTQVVNAHLERKLIDDTISDDEDVLAAIKPNDDKLARGTIKKYILPEDLLQFAGNTTARDLWVALAKKHIPNVRNFRMQIQQQLKDFEMLSGESIDDYFRRFYKIGYKASLISMKIEPEDALLHLIRGLSSDYDGVKSVLRHMESIESVDQLQDMLKTEEGELRLQRNKAREVSLMSLSRTHDGARVGPSQGAGAGNADGGRSRKACLYCNKEGHFIAECRKKANDEQRGIWRPALKGLRQKPRNPPALAAPPPAPPPVAPAAQPPAAAQAAAPPRPAQGQRRQLIALVYSSQQDDWERELWLFDTGAEVHVTGDKDKLRNYQHCGSDCKVWLKTANGSVVRPEGHGRVMFRSSCFETDVEVDVYHAPGIMGHILSLKALEGSFGPNVVPQFSGVLPHVEIGGAPAIELEKIDGLFYAEDSSYDSWMGRYGSDRCLSGKKVREELRKVKLAITKQDSNLGVLWHKRYGHLGFDALAKVASSDMVTGFGASVDDVVAAKGQFCKPCAEGKQTRQPHPSTGNVTSKRLDLVHMDLCGPLPVKSWNNAKYWVTFYDDCTKFSEVRCITDKTVVPETVKEVIKLWERQTGDKVRRIHTDRGLEYVNEELASTLRDMGIFHEKTAPYTPQQNGAAERLNRTLEECVRTMLADSGLPQEMWSEAVITANTLRNVSPTKGSNLTPWERFIGEKPDVSKLRVFGCPAYVMIPKQQRHKLDPVSVPGQFVGYAPGMSAWRVWIPKDRRVRISCDVKFDEWGVRGSMEFDASESEEEDVSGSDPEADEGNEIQEESPPEQPPEPAPQPEPELDVPIALRKSQRQNTKYAKSILGVNVDIPATYEEAIASPLVDFWIEAMEEEFASLQEKGTWSLVEKPADKQALGVKWVYSVKLDKHGVVERFKARLVVKGFMQREGVDFEEVFAPVSKHSTLRVFLAMVAADDMELHQLDVKTAFLNGDLEEEIYTVQPPGFESGGPGLVCRLHKALYGLRQASRAWNKTLHEKLIQMGFEVSNADPSLYIRRDLDGQICTYLLVYVDDVLIASHDEQTVLEVKEEIKQLYECRDMGEAEVFWGLVIKRDRETRTLTVSQEHMIHELIERYGMVNADPRSIPMSPGTILMRAADGEVLDRSRYPYIELVGSLMYITNCTRPDIAQAVGVLTRHMSAPTEQHWRAAKAVVRFLLATPQCGIVFGGERVEEGLIGYTDSDYAGCIESRKSTGGYVFTLHGGSVSWSSRLQRTVTSSTMEAEYVAASEATKEALWLRLLLSELGYSLRPTTINCDSQSAIKITKNPVISGKSKHIAVRYHMVREQVARGAVVMEDCRSDDMVADILTKPLPLEKFAKHQKSMGVCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEC9</accession>
    <name>A0ABQ5SEC9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF132">
        <property type="entry name" value="PROTEIN-LYSINE METHYLTRANSFERASE C42C1.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="349" mass="38237" checksum="C6ECB6D5EF0B4813" modified="2025-10-08" version="1">MRETWVHDIVTEHAALTTTGGIVWDAARRLCSFLEEMGRELGLSRPGLQVLELGAGLGWLGMTLARNLTALKMVVLTEQEEGGGLDWLRHNLLLNAHLPGMEVVKAEACDWRHFCDYNRIQQTYTSSQPSQSPPDKTGIPHTHPKSHPLREHCGLACAAESSSPPAEAHCLGGRRPLPEAEPEEVPDGCFAPPDRPVGGTEDFVGELDITAGDRGDGSTSGRGGCGSSDAWLLSQRWDFIIGSDLVYNAVGTECLPRVMRELAGPDTCILYCHTRHRFDTHDVEFFSQLTACGLVWREVREPSVPTPPPSPPPLTELFPEMRIAIYDIRLKPELVNSAEAVGPERKPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF52</accession>
    <name>A0ABQ5SF52_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012409</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045892">
        <property type="entry name" value="CrtISO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46313">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46313:SF1">
        <property type="entry name" value="FAD_NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="61"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="564" mass="61314" checksum="885DA31A8E59EAF1" modified="2025-10-08" version="1">MLSKHIGWEPTRTNIRARSNLTVSIRSQSRDAVAVPSSPMRQLTGDARSLETDVVVIGSGIGGLCCGGLLAKYGYKVTVCESHSIPGGAAHSWMRDGFHFESGPSLYSGMAGRGKEANPLGHVLTALGVELDLIRYNTWNVVVPEGNFLTRIGNDNFYEVLNKIRGPEAVAEWARLQEAMRPLAKAAALMPPTAFRYDPGVLFTAIGRYLPALIQSGGDAARLSGPFSTILDFADIRDPFIRNWMDLLSFLLSGLPANGTIAAEVAYMFNEWYRPDCYLEFPRGGSQALVQGLVEGLERHGGRLMLSSHVEKVNLDNKGAATGVALRGGGMVKAKKAVISNASVWDTLKMLPPDRLPEQWRRTRQETPACPSFMHLHIGFDATGLDPSLELHHIVVNTWTGGVTAPQNVVLISIPSVMDPAMAPQGKHCLHAYLPATEPYELWKGLDRRSPQYAALKEERSQVLWAGVEKVIPDIRKRAELVMVGTPLTHERFLRRHRGSYGPAIAAGKGFFPGPTTPIPGLYCCGDSTFPGIGIPAVAASGAICANTLVPVWDHWKLLDEIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGE9</accession>
    <name>A0ABQ5SGE9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012794</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF96">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="888"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="979"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="298"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="381"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="521"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="696"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="755"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="416"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="457"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1114" mass="119992" checksum="F728CA5919D5BC39" modified="2025-10-08" version="1" fragment="single">MAVWNDYTWIVVTGCLAAFFTAYGIGANDVANAFGSSVAARTLTMRQALLIATVCEFSGSVLLGGGVTRTVASGIAKLTAFEQNPEIYMYGMLCALVSSGTWLIIATYLELPVSTTHSIAGAVLGFAFVYGGPKAVVWLEPQEEFPYMKGMVPIVVSWFVSPLISGLATAFLFFLVRTAILRRERSLDLAFWALPLLVLFTVFVNVYFVLYKGADTRVKWSSNKCAWVSACAAGGCTVLTIIFVMPLVRYYVYQDIEARRLQQLSEMESHTRGSSRNNRTCGSGGAYPVLHNHSIRSVGAPPGGGGVELNAAGSSGSGGSDGSGARGGGNDGSGARGSGTMVAAALTSLQAPGAATLVGDCRAPPLPPPARQRHDYAAQPPQLQTGTESGSSVRQQQTGQPMSPPSVVPPTGLAHSSYSSLSRSQGNQSLPLSLPQEQRLVQQQQKEVWEPPPPPQQQQQHQQHHQQQQRGAPGLVMVNFDGGAQINEEYGGSARYVSPPMLPRQQTSATRVSVDDTHGLAGGASGTISSGSGAVLGDHGQLQGRDGSLCLSTPQQMQMQQVPSWIPDMQQQQQQHQQQQLLLQQQWLLQQQPYLQLQIQDVRHPHQQWALPAQLYPQELVQQHPGMQQQDEQQQCQQAPAVQMQRLPPSQQNPQWMLHQIASMQSTPSMQSMQSTQLLPHQQVGHWQAHIDTSSANTAAGMKQGCDSSMHHQYHHRHQSSGPQPPPSPQIQTLTPPGPIAESLEWGWRSVASSSYENRHDKRAVRPTGGGGAAAVTADAAAGNGSGEAPESNVQWQNTFDQLKAIVLRGTQVKVHEAILTDPVAQRMHTYAEVFDPATEDAFKYLQVVTAICDSFSHGANDVANSVGPLAAIWYIYRFQRVDYLSDLPIWILVIGGAGIVMGLATYGYNIMRAIGVRLSVITPSRGFCIELSTALVVAIASKYGLPISTTHCQVGATAGMGLMEGTSGLHWSLVLQFFIGWVVTLLLTGLMGAALFASGAFAPSILQSRDLQKYETAILNLATKIDLLVNRTNYAALSDPRTWGNFSAALNATVTTGIQSLQMYGQDASTANGTRPVQHIDPSPFLELLETTMSVYYNNTLPYIGGMTSKGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGM6</accession>
    <name>A0ABQ5SGM6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prolyl endopeptidase</fullName>
            <ecNumber evidence="6">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_013161</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Serine peptidase whose precise substrate specificity remains unclear. Does not cleave peptides after a arginine or lysine residue. Regulates trans-Golgi network morphology and sorting by regulating the membrane binding of the AP-1 complex. May play a role in the regulation of synaptic vesicle exocytosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S9A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.120">
        <property type="entry name" value="Prolyl oligopeptidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023302">
        <property type="entry name" value="Pept_S9A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002470">
        <property type="entry name" value="Peptidase_S9A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051543">
        <property type="entry name" value="Serine_Peptidase_S9A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11757:SF19">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11757">
        <property type="entry name" value="PROTEASE FAMILY S9A OLIGOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02897">
        <property type="entry name" value="Peptidase_S9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00862">
        <property type="entry name" value="PROLIGOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9A N-terminal" evidence="9">
        <location>
            <begin position="113"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="8">
        <location>
            <begin position="591"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI68675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="832" mass="92866" checksum="8A3B520AA15EC751" modified="2025-10-08" version="1">MRPSNVVVHITAAAASVVVGFTLGRRVGGINLKRQLNGNDKLLSRQERHLATALIQMGYGHLFSYWPRPGVRDAEKRRLLAAVAHQLDGVSASVETERLALLAPPPPPQRPKLLQIHDDAREDKYYWLRDDDRKDPDVLSYLKAENEYTKGVMANTEKLQEALYKEMRARIKEDDRQVPSRHHGYFYYTRQETGQQYGIQCRRRVPAAMTAQGPSEADDVDESEPEEILLDQNQRKAQLGVDYYSVGGSDTSPNQRLYAWAEDTVGGEKYTLHVKDLTTGSEVIRPISGMSGDFEWAADSATLFYVVKNELDRPYKVLRHKIGSPPGQADEVVYEEQDEAFYVGLYKGRSERVLYISSGSAVTSETHYIMADQPQSEFKVVLPRVQDTEYSITDRGDWLFMTIRDKARPNSELLVAPMSKPTEGQVLIPHSREVKLEGISLGERYMVVSERSGGLQRARVYELPANLDRPTGPLGAGEEIKFNEPAYSLGAYLTGDFDTPLVRMSYTSLTTPSTVIDMHIGTGKRCVKKVAPVLGGFDQTRYVTERLWATAKDGVKVPISLVYRQDVVKLDGTDPLLLDGYGSYEISNDPYFSSSRLTLLDRGVIFAIAHIRGGGEMGRYWYEDGKMQKKINTFTDFIAAAEHLVSERKLVRPERLCIQGRSAGGMLMGAVLNMRPDLFHAAIIGVGFVDCLTTMLDETIPLTVIERDEWGDPAADPAVYAYMKSYSPVDNVKAHRYPHILAIAGLHDPRVGYWEPAKFVAKLRELQTGSQLLLLKTEMGAGHFSVTGRFEKLKEVAFEYAFLLKTLDMLDVPLAGTQPTTEAAAAAAGSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGY0</accession>
    <name>A0ABQ5SGY0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histone deacetylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013190</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF25">
        <property type="entry name" value="HISTONE DEACETYLASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="3">
        <location>
            <begin position="251"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="703"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="775"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="651"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="717"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="818"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="837" mass="86725" checksum="591F0138629B64F8" modified="2025-10-08" version="1">MIHCPNLLPRPAGPHLAPINALCAVTRLTRSTATRLPLGAALPLVTTTARDGPLTLCRPHTTVCTNASASADMETAYDGLEHDDGDDEEFIPPPLPTSALELLSEVLTGIAALSMDDSTSGASTFNNAPSSKSLGAYRPLRFCVNCCRPLPASGTMCGGCGHDPCRDVEVLGDPNAWVQAYAEQLQRQQQQQQGRRRPEHHQVHVSSCAAAVPVTTATSPVACGPVMHSNDVLLAADPRTLLHRSSLPPYPGRPERLQAIMSRLHSRGLMERCHLLTCRPATDSELLRVHSPQLVAAVRNMDGTHHHDGSCSCSCGGDRGGNSSSRIATSSAGAEATADGSASGPAHDVGVAAGIAASARPLCADTLYNAHTCTAALLAAGAAADIGVALATGEVRAALAVMRPPGAQAGVDVARGGCYLNNVAVAAAAALASGLQRVMIVDWDVHHGRGTQEIFSEDPRVMVVSMHRYDSEIYPGSGSVEEVGERQGEGFTLNLAFARGGTSGAAAGAPGNGGLVDGDLLSAALHVVMPVAYQFRPQLVLVAAGFGALRGDPIGGCSCSPAVFAHLTHMLAGLAPRGLGLLLEGGYNLAATSQAVEGCLQVLLGEAPPPLPGPWGTTSEGWVAIMNAMQVHSHFWSALHPLSFHGWTSAIANQQRQLQQQEEQDEQERLLALQQQREEEEEEQAEALGAHYYGIQHGAAGGPSGGGGWAGLRDDDRCPEDEVLDDDGGSDDGLSPEVLAHVAAALQRTREGWALEHGGRGGDEDLAAAMEAAELEDPRDIDPTELLDAFLGMGSSTSRQEESNSISSNGGGDGGSSSTGASTAAAAALEVHGEGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH13</accession>
    <name>A0ABQ5SH13_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-mRNA-splicing factor ISY1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013175</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="31405" checksum="438E7E3640DED130" modified="2025-10-08" version="1">MARNEEKAQSMLNRYLAGKLDEKRGPKQKRPYLASECRDLVEADKWRSQIIREIGKKVMEIQNEGLGEHRIRDLNDEINKLIREKGHWERRIKELGGPDYSRASGKVTDSKGREVAELTGKGRGYTYFGAAKKLPGVKELFEAEPVKVARRSRHQMYRAIDPDYYGFRDEEDGVLERVEAEAEKMIRMQAVLDWEEKEMERQKALSAVATHGSAGGGAGGAAGEGDEGARFIAYVPLPDQQAIEAKILEMKRNDLLAKYTSDALLQQQAQAKALLNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHC7</accession>
    <name>A0ABQ5SHC7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Centrosomal protein POC5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein of centriole 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_013311</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Essential for the assembly of the distal half of centrioles, required for centriole elongation. Acts as a negative regulator of centriole elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033351">
        <property type="entry name" value="POC5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28618">
        <property type="entry name" value="CENTROSOMAL PROTEIN POC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28618:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN POC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="450"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="589"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="332"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="413"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="491"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI68809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="612" mass="65469" checksum="5FEA4144FB57596F" modified="2025-10-08" version="1">MSASTNPYLVGYEQFLRKPNLDYNRTDPQPSSAPPTHPVLTTSVSARTPALVSRTTAPVTEPVQPTVQLHPSTNLDDSSGIDIFVPMPPGGLSTQLPPSDVVNFAGATTRPQTPTTLNIIARPSGVTAGQAESAAVEITISTQPEEPQPYVSHLQGLTVQQMLDLDIDTLVVKIDEHHNQAKKAVLDHVMETKARLMQAKNDAVEEEKRKGAMLLATKEEEINLMTVEVGNMRLTTQRLWDLLGRCCAAYGTAKERRRVNLVQFQIFCAWRRQAMMLARRRRLLARAERWDINVHLKRNVFRAWFREAIRAHRVTLNNRYIQEVDNAKRLIHEHYHRQIAEMERMLADARHQLDREAETRARLEEDMKRAFMRGVCALNIEAMNMMKRGAAAGGVNPANGAAPTAAEKQPTEPAAGGSVASSAPASAPHVSAAPAASEVVNPDLCRSITLSPPRFTAQPGPGGAGSSTCPGPASSAQPLAASSTSAVRLSGNGTTSTAPTVSTMLTGSSSSRPGSSYGLPVTGSTMTVSGNSLVVGRASAGLQQAAAATTGQGTSTSGAASEGGPTVRETAQTFYQRPQVVVTRGPGVNLAGSSTEVPRPRTNAPLPTVRML</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJN7</accession>
    <name>A0ABQ5SJN7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014418</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002410">
        <property type="entry name" value="Peptidase_S33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051601">
        <property type="entry name" value="Serine_prot/Carboxylest_S33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248">
        <property type="entry name" value="2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248:SF2">
        <property type="entry name" value="PROLYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00793">
        <property type="entry name" value="PROAMNOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="129"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="525" mass="58069" checksum="71007115A9EE6FA4" modified="2025-10-08" version="1">MLSRTVVNGTLRQLSDIRSLPSRLHRIVPSPSFLSKTQSRVKYKPLTSMDSLRPAPACKRQRTSVEVEGVAPLHEAGKTFNVPGLVVREHTFTVPLDYSNQAGPFKGQTITIFARELLAPVRNETLPFLVYLQGGPGFEAPRPCEASIWIKSAVNSHRVVLLDQRGTGRSTPITTANLAKQGGPAQQAEYLALFRADSIIRDCEHVRKLLVPLSNFGGKWAVLGQSFGGFCATTYLCQAPKGLMEVLITGGLPPLVTQPCSAEDVYRALHERVLAANAKYYERFPSDVELVTRIVRYLAAQPSGGVQLPSGSHLTPRLFQTLGLSGLGTGGSFERLHYLLDSFFDADEEMTPAFAKNFESWQSWDSNPLYALLHEPIYCQGRDSAADWAADRVRDSEPFASAFDPVAAVTENRPVMFTGECVYRFMYDDIAALRPFKETADVLAEKSEWNMLYDPAALSNNRVPVAALSYLDDLYVDHDMAMGTAAIIKGLNLWVTNEYRHSGIRDDGGRIFDRLLGMARNAVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJW2</accession>
    <name>A0ABQ5SJW2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014695</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13971">
        <property type="entry name" value="ADCK2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044095">
        <property type="entry name" value="ADCK2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052402">
        <property type="entry name" value="ADCK_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890:SF1">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2 (PREDICTED)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="459"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="74"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="851" mass="92050" checksum="72161518CC2FA7DC" modified="2025-10-08" version="1">MSRTANERQRIFGVQVSEAQARLIASAALLTALHCRGTRSPALQPLTSAFSLRLSERCGQQLCCARGRPSCGPLPPLRATNPRAANSLPRTSAATSANPSPPGLFTNALRALREKPRHAPDLPGGTRNSKPPASTGLSSGTALSWSSGPAAAPSRGGPHCLGYGPAGGRTPCTTAGLTPPIASSPLNGSGSGVSLHRSPNLFRSFAASAIRLPPAASGRCYSHTAAAAAATAARHTRTGWNASQAYLSNVQKSVLETMAPYWGSLSWVALVALYKQSHAYAWGLPSVRQLHFFLLSAINVLAHNLPVTLALTVAEMARSSRCAQADTFSFYLDVPTADPSDRPWWQALPLQMAALLVSVGRSLWLLLLFAPVLLSAPVALQLQFRRAEWIELLRKTLEAAGPAFIKWGQWAATRHDLFPPDMCSELEKLHTQAPGHSFRYTQAAIERAFGVPMTELFESIDRQPVASGSIGQIHRAVLSDKGAVMTGVPPGSMVAVKVRHPGVDAAILRDFATMLAVAHLASLLPSLKHLRLEQTLSQFAAPLREQVDLSREAANLRRFNHNFRKTSHVFFPVPLYPLVTPDVLVETFETGEHIIAYIQSDHNPYNHRLSELGSGTMLQMMLVDNLIHSDLHPGNILVRLDPPGGLLGLLYSALDKIRQLQSIAPANRARIELLQQRWLQPSLVLLDVGMATELSPTDQVNMVGLFRSFAAMDGRACGDWTLRFSGDAQSCPDPEAFRVAMQEAFEELHKMDLAAAGSQGEAACTFKNGADALASVLELVRQHQVSLPGHICAVVVTTLVLEGWSNKLDPDHSVLTQVQTMFEPVNVAWAHRITQLVDRVMEEEANHFALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK91</accession>
    <name>A0ABQ5SK91_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014534</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000088">
        <property type="protein sequence ID" value="GLI69829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="4">
        <location>
            <begin position="33"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="17072" checksum="644CC5947BA32A3D" modified="2025-10-08" version="1">MEPVVTVDWLSERLNDLSVRVLDACWYMPVHQRNNHADHRAVRIPGARFFDIDGIAADPVTARGLPHMLPSEQGFAAAMDALGITNDTTVVLYDHLGIFSAPRAWWTFKVFGHDKWVIRPLGSSSVFVCLCVCVFVYMFICLYVYMFLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKP8</accession>
    <name>A0ABQ5SKP8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="590" mass="62122" checksum="209089C1E3D70939" modified="2025-10-08" version="1">MVFAREAAVIIKGRDGRTVLLQSRGMDFQVASLAAGEAMAALQAAAQAPESWDVLSNTQAVPFLSGRLEVMHHKLTVTYRVAGGLIFLVVTAPAANVFSCIHLLNGIVRVAAVQADGKSAELNPERLQRRFGEVYMAVDALLSSGGVLDPNTALGRALATLEQLQDRDKGKGVAEAAQKPPQQQRPGGKPFTRRTLQGMIDQLALLSFSAGPAMQGIQARPGFQLQEAVPQAMSRPKQEEARDPFPIDLGSSVPKVPRGKDPNDPFPDLDWAAPEPSKTPEVVTTTPATTTTTTATMTTTGGAGGAGVNVDPVPVAPPPVYAPFVLSEPVLRLVEIWRGETSAGQLVRAGVSGRVEWISEAAKAKVHTVQLMLQVPEASCEHLPAALAASRRHPDCCRAGPTGGLLLADGIQAKRHEGALLLYYHLPPLAIRLPMQAQLGASILPTQDGRHLVTLGLHYAVNPVLAQRAAGLALEIVVPALLERPLRTSPPGAVFDAKARTLRWQQPGPVSYLDNHGTAVQPFLASFAVDEAVASEAALPAALVRLNAKLSLLGADGSGTLTGASLAQGVVDLEVTPSLCGWRAELTATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKT1</accession>
    <name>A0ABQ5SKT1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014879</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033275">
        <property type="entry name" value="MARCH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012">
        <property type="entry name" value="RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012:SF215">
        <property type="entry name" value="RING_FYVE_PHD ZINC FINGER SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="6">
        <location>
            <begin position="116"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="73"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="345" mass="37773" checksum="7A15E83DE516F4F2" modified="2025-10-08" version="1">MRNPAVSNSGIAEETERLLGSRTNERLERDASNGSAAVPALPTSEEQQHGHQDTDEKRGQQCVLFIPAAVQQPASTSGRNGASGNSPRASGHARTASMDSLKSSSSGSGPSSATVAPKGESGLCRICLEEDSLNNLEQPCACAGTQKYAHHECIQRWVNEKGHLRCEICDQQYRGNFSLPPQGAAGTEDAGNMLSSMFAIRVDQGGEPLGAQHDRAALDFLDESDHYYQRNPLASWCFTFVIFVMFLVVLHHTMIVADGMDGSTASGDESEDYATSLTLFLFWIGTKAFLIGIPLYTVMRIAARQARREQYEAMLRTGAFEAPGRRYVWRMQLRDQDRGHGQLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKU3</accession>
    <name>A0ABQ5SKU3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014916</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="236" mass="24444" checksum="05D89F20F372A161" modified="2025-10-08" version="1" fragment="single">GLFRMQIPMKMIPLEAFCYDAYGLYDHRPWTPAETEDVGLAVASTARGCNEPGPIKTVKTSASMEAVHDPCTSTQLADVQHRNGPLSRKRRFEEMAAEQQGLAGREGVVAAEVAVATEVAVVAVDGAAGAVGDAAAVAAGVETGVEAGLDAEAEEGQGEERATRQRVANAPDGKPVAVFRGQRPRPRAASVMKVWACGIMAWAVPVVAAAAVATGGFGLGEAVCEVLLAEGMAGWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL06</accession>
    <name>A0ABQ5SL06_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_015256</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 40S subunit of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the 40S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038582">
        <property type="entry name" value="Ribosomal_eS31_euk-type_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001669">
        <property type="entry name" value="PRK00432.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="15"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="166"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI70306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="196" mass="21186" checksum="39BFDC9B358E0197" modified="2025-10-08" version="1" fragment="single">RCKEGTGITAECRKMQIFVRTDRTHLLEVSPAQTVADVKAAVEALQGLEAADQRILFNGIQLEDSQVLTEAGVSDDSTLMCMLRLLGGAKKRKKKTYTKPKKQKHKHKKIKLRVLKFYKVDDSGKVQRLRKQCPNCGPGTFMATHFDRVYCGKCGNTFVYEGGAPAGAPKPKAAAAPAASAAPEAPAGKGGKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL39</accession>
    <name>A0ABQ5SL39_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="175" mass="18927" checksum="1A3B0D5E8D3A7138" modified="2025-10-08" version="1" fragment="single">MISLLVKLGFKPKDRAYERIDTISDTNSTANDNDSVMSNSSCLNGKAVEQPAGGVVVTPLRELMKKFSRKWIPTEKPLQRFGKLEGEESLTLQERLSARRLQIAEKGTLLKVLLSWGAPENLHQAGAQLEAQPVPAAQQEPQLESEENNVQQCAGGVMAPSGLAGAAAAIELAYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL42</accession>
    <name>A0ABQ5SL42_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_015736</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF87">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046063484" evidence="12">
        <location>
            <begin position="23"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="599"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="620"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI70713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="785" mass="86018" checksum="6C38A8582B7D633D" modified="2025-10-08" version="1">MRSIVFLLFLPLVGSLIQRSKAFPHPNFNCRKIAFYKTHKTGSTTLGGVLFRIAVASNKSIYTTRNSHYVSLGFRENEQLMGRTDMVLRHVRYSEAPDFHVATRNFYGRALGTTEYGFLTILRHPVDRYISDFYYYTEPEARKAHRRLTLRQYAAKGEGANRMAREFAVKTSAAALEFVAGEMHSDGLFLILELLDYGLALLASHCGWRLSQLLYLPTNTNMRGAARYGNVTLSARPGIAQLFTEDPALLKHIAAINSVDAILYGAAYGHLLATLQLYSPAAAATSAVMTTAVRLTAARKQLEADCRALNKDVAAATAAENVELASFAPPPPPPPLKPRQNISVNDLQGLCAWYSMLDSLYERMPNLETGRVDLKTMLPPTSTTSPSGPVGHLLRLADEYLAPYEDSSMPYYHSLIAVHVRHRTSAEAELVYGMSSDDGGDRQLAVLAAAVRLWTSHHAKEGLHHLVFLVDALNDTRHEDLVRQAAGAALRQSGGGDDATADVAPYTTFSLLRCGTASNANINNVICGGAAFLDTISRSRWFAVLPDLTYFMYGTRGSVRSVLLSYEGTLQAAGAICAPLVPWRTPPLGAVLIWPSGGAAAGRRDRRGDGSGTDRTVFDMNDDTEGDDTEGEKEGDDFQADHGAGVSLLQQEQQLRRWRRWRLKEVSDDDGVGVKLTAASTATPGCMRTPCSLGNIKDSLPCVMVRAMPLLGLTQPYTTSGTVLPAGRSSYKVPYRGPDQQRLEVSKLGPPSEKDLQQLAENPNEKFRCAKLPRPKLWLELCGDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL54</accession>
    <name>A0ABQ5SL54_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015020</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="362" mass="39266" checksum="F7248834B21B8B2C" modified="2025-10-08" version="1">MQLLCSSAACPQEICTAIEEASCLSDAFETCRPLAYHSDCYVSVAKCKCNGSELVVKAYLRDQLSPGAREQVQTEIDIHSSLHHPNIVQFLAAFEDYARVYILLEYATGGDLRKQLQGLSESRIRDFFIAPVLQALNVLHNKGFSHRDLKPENCLVSGSETVKLADFGLAQPTHIPEDACAADSDSCAVSVGCSSGTRSVDSEYSSSSSRAPAVICTAGGTPLYAAPEVLKAMFRNHGMQSAVGPKNDVWALGVMALEAVTGCHPFSPDHYHYENVLYSIAHCNKVNLPSNLSPEFTDWLEQALNRDPSQRASVAELLAHPWLSKQYSAEEEALFSRSGQTNQGGSALSATGGDIGFDCWEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLA5</accession>
    <name>A0ABQ5SLA5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="267"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="429" mass="46919" checksum="8231A15E610221F9" modified="2025-10-08" version="1">MPHVLSCLFWPDMEPNKKLERTILVMMLTTILTSTSHLWRTSATAQDALEASQRVLLKTSSSSGSSSPIKVIHPPMPPPPAPPRKLRRPPPSPFPPPSPPLISRVYSPPPRPSPPPPYDVKSELFLPPPPPIPCTVCVSIGIDDNGMSVPFTSHADCYNVGNSIIQDMTVEADSVRARIYQSDGPSIDCSRGDLIRVCFKFVSNDDGNKLQKYIGARMIAWPYLLHYNLCEKVPNASVNIFIGGEGADPHATSCLQDERRVGCLAGPKVCTETQGATPFGVRPRLTKLPGYSSNTVMYCFEIVKVPVVHTNTRCGNSTVLRKAEITVGNDKGLVGMYIKPAQAANMKAIQPIWLPASMTLVVYIEWTLKEADGGHICLELYKTTNMASFCLGANNQGIPRCMINLVDPSGDCCPYYQPKVGRFNTIPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLF4</accession>
    <name>A0ABQ5SLF4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015376</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046968708" description="Guanylate cyclase domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="3998"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="3">
        <location>
            <begin position="863"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="757"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1088"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1144"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1215"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1317"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1406"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1684"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1898"/>
            <end position="1997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2037"/>
            <end position="2094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2231"/>
            <end position="2254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2464"/>
            <end position="2503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2522"/>
            <end position="2580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2668"/>
            <end position="2692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2740"/>
            <end position="2760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2924"/>
            <end position="2995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3108"/>
            <end position="3129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3217"/>
            <end position="3292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3401"/>
            <end position="3485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3509"/>
            <end position="3542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3816"/>
            <end position="3837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3974"/>
            <end position="3998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="767"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1105"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1148"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1275"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1419"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1695"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1968"/>
            <end position="1997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2037"/>
            <end position="2054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2062"/>
            <end position="2094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2464"/>
            <end position="2486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2488"/>
            <end position="2502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2543"/>
            <end position="2569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2570"/>
            <end position="2580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2938"/>
            <end position="2947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2971"/>
            <end position="2989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3217"/>
            <end position="3228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3239"/>
            <end position="3248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3263"/>
            <end position="3279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="3420"/>
            <end position="3434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="3464"/>
            <end position="3473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="3513"/>
            <end position="3527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3987"/>
            <end position="3998"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3998" mass="412944" checksum="A2A53D9B8A7664FF" modified="2025-10-08" version="1">MKPNRLLIIHFAIAIGVSFTVAVDEAVVSPCTQCIASSINESCAAFDDASTIIALEVIKGVLGCQLLPGNAEAQSGLRVLLPDSFAIWLSPILERFSQLYHVPLNVTIVSLSEWLHLDEDPLGEQSQLSPPLPALPAEQKQLLQLPQPQQQQEQQGATANELPAQGQFLWAQDVALSYDVLLHPATSTLQMADQGDVTDIGHLVDLVNSNLQWHGISAYLRLHLGSYQGATVGLPLDGSVLMTMYRSDVLGELALEVPSTWTGLLDFLTAYDRERARREGNAPSDAASAALPPYALCLPRGAGCSRLSYLTAVWSSIAQTHGSQQGVHFDPASLQPQLDTPAAAKAFRVMWQLMAAAAPSEPGEGCSHGSLGFARGRCALVLAAFVPQMRMFFDAAAPGASNGSSCKNSSCESSNSPSSTGTGTGSVGDIRANNNSMSSSDSLAQQQGLIRAARLPGSETVWVATRGSSAGALVNCTEELCPHAKRIGVGDAGDADVGDGRLVNFAPLMLESVLVGSVSNRSNIFTQFIAYELLSYLSSPKRYDFDAAVATDTPSPAARNDSLLPPLPPPVLPLVSVSPVRLQYVSPQSSGLWSQAGYDTGTVTSMLPIMTEALSSANLAMGLRMPGGGFYKALLESILAGIQSGSIAVTRTQFDSDGGVLSLHQQDPNQDPRQQLLQKQQRQRQRCRQLRRHLTVDEAEPRLAQATVAAVPQLSSDVRTLAAALADGQAELKAHYNPDAYLNRYRASLGITSLPSHWEPGSGGGDSVSNEGNSGVSQHSGIPVTAVAASVSTVLGCGLLVAAGAVLVLRQGRSGRRMRRRLLQLLTPLCGSACARFHDSSRTDFSVSAHPQVKAPVASCQTAIVVTDIESSTKLWETLPADVMSTAVQLHHACIRLLLVEHSGYESATEGDSFILAFRSVKQAVAFAMDLQAQLLVLPWPHELLQDEICAPVYTDLPEGFPFTHHHFDTSANANASANANANASASASANANASTNASANATAASSRIPDRRRLGLSLGLGLSLRGSVPVSGGSGLLSIAGSASECLTGECTRTISHLGPEPQPLQRVDTSPPSFNVASLSRLTVAPIPPRSRSHTALAVPQVSGGGGGGGGSRSSAQRAWRVSVRASRGLLRPSPVASVSEFEAGAHGGDGGGSGRGSGGSGADVGGDRSRSHSHSNNRNGCGNLGMFRSTFSALLIAVTARAAVTGGTMPSVALRHLRPPPLLPRTRLGRGSVPRRVPVSAETCEAGLPPPGDLSVRSDLTDSRRSGGCGNSGANSGANSGADNDAADEVDEQQAVGLHPGDVCRTLLGGQAASGRADELSSDEDGDPNSRCARSPLPLMPSAAAAVTAALLPLPPLAAPDSELAFATASPNISIGGYGPLVPSAAPPTGPWVLSNSRVRVPSAPSLEGGDGGASGTSPIGPTASGNRFALLSETAAVTAAPAPADAAAGVSEPSVIAPADPSPPMPSVLCPWPPAGRTLGQLLSELKRPDASGIDVSIRRSLMFRGLRVRVGIHTGVSSEAEMQYNTIAARIVYGGPCVATCKAVADMAHGGQVFLSGAAKAQLEEAVHHHNGGRPGGTLVLHIGTFVKPRLGTQPGPPSFAVANAAAVATAASTLASPGLCSGDELDPHVVRNRSHASRRTSRVDMYAVRLAAPGVQAYSYSAGCGKCGGRGYGEVTTEVATTRTSPGDRVSDGVRDQRLTSSVPLPRKHLVPEHLQHTASSASPSPAAPMYATAATSSLEASLPQPPPPPVTAALLECQVYWVTSSSLAQRLAIVPPPRLPADLIALKDVYAAPVGRASYVVVQVPAVATLVAWDARVASDALGLFYDTAQAQLRQVQGGYLACPHPGELHAAFTTAVAAVRWAEGLRIQLLEAPWAEELLSHVLCEPVEVSDSDSTDLDSGSEGSVADEGNSSSADVGATPGGDSSAAGRQPPPPPPSEAVSWRSRISEALSAASSLHQHNQQHHHHHHHHHHHHHHHHQHSYTYHCHHHHQQRLVFGRMPSPSRVDSSPQPSAGGLAAWISSRLNQTLGSTSPCASGGASPAAASPLRRHTNMSTSPPGTPTVTTNGTGTATSAAPNLMPWQSLSPSVPPPSPFVALAGTASIPSMDGSTTGISGSFGAFQHIPSRLGLMHRGRTADAIAPPPTPMPKRRAATEGISAFTNAAQRKGTSSSFKDAVNYHYDKLLYDAAAPRRPASTSTVVPYVTLHQTVPDINAQPRLQRFSSEGASLSPLGSPARPSSPRLNGCSSGAACGRDAGDSSGGCMTGTVTGQVGESAAATATPNLLGADVHATAGAADTCGQGGFYRANARRVVVQYAGLNQTTSIVDSSHLLLLQSMDVPLDATTEVATAANALLLSSVDHFPSASQHNFHDPAQQDMQPTMSACCSNGPADRSHNLLHSACLYRSNGDRSPAAHHFHSSSLDVELRNGGAASGCQVSEVSEFLRCSHHRAYHTRYQPSSNSFVRQGANSAQREGTSTDAGGRGGGGGGGGGGGEAAIDVTAAAEAAEAVEGLDEILPQRMPPRGLPCEPSEYDSLGGGADDGGGGGGGSSRRRAAGGGGAGSSSAQAGTRRSNRLLLRGLRVRVGIATGPISWSVQDHTHDIGYAGKPVRLAMRLAGSVAASGQVLCCEVTCAEAVAAQAAMAAAAVAMAAAAASPAAFNPEPTTTSVPGQGQSQPQPPQPEEQQYGAVALTSSGGSGNRDVMYLRSPPPLQQSPEMAVLGGSLRHTISGLTRRTGGLSPTTAPIVATPAIPSPRDPGMATLAGSNGDMCPLVAFSPLHHRCSIVHKRGGKPSIFVCSFYQSLRPDASAIRRQLAAAIGWLRPAPLPPTAAPPPPPVSAVTMGGRGFMSGGSSPGCYATAQCLSSSMHSTLPQQPQPQPYTNLCSRQRSPTATLAAFRASSPPTFDLRTVDGSPGGLIPGPEDTLWPASTRPSTRQSPSGLLPIGELHIGDDDSDGSDAERMGSGLLSSPLGLPVPGTSGTRSSASLPLPRVSELSFGNLALVNGRPSVAPQTNPCGSRRRSQALQLPVAGTYSCAPVLVTDTGNRHEHLQGLGRGCIRCNDVTASGNGLDAKGTLGSGALVEEMITRGTGFRSTLLMGSSPGGSDSTLPSGATLKSARHRTCPGTPPLTAKVAAAPVDVICAHRASRLFRASLASGEQRWMLQPPGRRVSMESSRGVADQVIGSRSGHIAGVYREHQDMQVLQQPPAARPLPLQLPSPPHQRADGLLFRPLPPRQPPPQRDLQLLKSHQELAMQQQQQQQQQQQQEQQQEQQEHRTHGSLLGQSLSSPSALCHLVGCHEETCPRGNGYSVNSAAGIPAASFGTTTIAESVTVDVKRFMSQQTSPTVLATATDATAASAAPNVQTFMCSATHCMKAVAQPAVVATGSSASISVQMVESPDTRDRSKPGRIGTCSTHSGRTETQLPSSHGGAEEGIVGDADPFRTVGPGSKGAHGWGEGERGKEQEGDGDGPEYSLSFPASTLRADLGGSTSVRDILDAGVMPAIGDRDGGRDGDRDGGRDGGGSGAAVDGRSTYGSGADRNFRCQVINRRLWPSCNGSSGGGDSHAAAGAAGGCGMVAGGPAALQPRVACAGSSQSVVQAAQRKDGLNDSSKELPVSVPLPLQHPPDLKLSAECSGWREAEKPKLPRALTASNGGHAGAVGIPGDPLLRYLTEHFGLHTPPSLPLPQLMKPDLLPVGQQQQQQQQAIRLAGMWQTRKGWGVAKAVREGIVTGTWRQQPWLSSGADGDDDGVECDHCDLRGGADGAGDCTGSCSVRDLGEYGSTSGGDDIAPLQILPAVQDLERTVDLAAGDTPGDDADKDNGDGCSGPDGLSLLPAPLPLCLPLTQSRQGKLMGSDLGSPECTAGACIAGPTGSRSVWRLAGLVQGGTLAGWSMVSSSAADAGAVEDAVSGTPGGCSAVSAYCFSAVGWSGGVTISESQQQQQQQQLHHDSMSDSELEVTARMLVSPGLMASDRPNAVSSSNSSNSSGAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLI0</accession>
    <name>A0ABQ5SLI0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_015149</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF119">
        <property type="entry name" value="NUCLEAR RNA-BINDING PROTEIN SART-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="277"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="375"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="482" mass="50379" checksum="8453821916293F62" modified="2025-10-08" version="1" fragment="single">MGKSVAKASGKELKAKQAAVKLEKKKVPEPSDSSDDDDSDDSDDSDDAPVPVKKAVVPAKATAKKVEDSEDSDDDDDDDSEDEPPKKAAATPAKAKVPAKKVEDSDDSDDDDDDDDSEDEPPKKAAATPTAAKTAESDDSDDDDDSDSDDDEPPPPANKGKPAPAAKATAPAAKKPVAKKESSEESAEDSDDDDSDDDDDEDGDAKMADAKKSVPAAAAKRKQDDDDDDEEEEEEEEDSDEEEEEEEKEEPKPVAKKQRLDLAGNAAATNGNSGESARVFVGNLSWGATEDHLRSHFKDCGKIVNVRMGVDPNTGRSRGFAHVEFADSAQAQKAVAKAGTEIEGRAIKVEITQPRNQSFGGGGGGGGNTGDADGTTIFVKGFDVNYGSEDDIRGALEETFGNCGTIKSIRLPSDREAGTLKGFAYIEFDSTEAKNAAGELDGSEAAGGYLKVDVNVARRDNSSGGRGGFGGRGGFGGRGGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM95</accession>
    <name>A0ABQ5SM95_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000102">
        <property type="protein sequence ID" value="GLI71112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050228">
        <property type="entry name" value="Carboxylesterase_BioH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194">
        <property type="entry name" value="HYDROLASE ALPHA/BETA FOLD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN LPX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="126"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="408" mass="44322" checksum="2FF99763E45743D7" modified="2025-10-08" version="1">MNYVRNEMPYLPLSKKRRGPTREQPLQSKFIFYNYFLILSRAYRDIPQSVDKAAMQARHRPFSGRRNPCTARLSRHRFVTLAAVGASTPPTAVYTTKHTLPDGKNLELLVQRAARSTSSSSPRPPLLFVHGSYHAAWCWQENFMPYFASRGYDTYAVSLRAQGGSDPAPLGQSVAGTLDLHASDLATLVPAVQQSSSAANGSSGAGRAPVIIGHSFGGLIVQKYILSTAKSGTPAPPPAPGAAPAPGAFPPLSGAAFLCSVPQSGNQQMIVRFLLRDPIMSFKLTWGFIARSFATSREACRELFFSKDVPQDKLEMYQKLLAAASPTRLIDLKDMNAQVPLPPPPAHAPPAFVLGGADDTVVDPQAVKELASYYGIKDILLQGVAHDCMLDTRWERAASELEAWLATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMH7</accession>
    <name>A0ABQ5SMH7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="764"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="874"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="971"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1100"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="532"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="773"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="799"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="811"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="971"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1013"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1102"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1124"/>
            <end position="1135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1347" mass="138103" checksum="03558E07DDD1DA1F" modified="2025-10-08" version="1">MFSEQRRLGRGVSDGSRQPPTCTTHSRKIAFHTAQLQHLISFKRILFDPFTSRHKSGAIAVHGTSGKDNQSSRRVFTEEDSDDQQSVDVGSWWQADRQTDEAGPQHGQRHHLQQNQQALPQLNRNITALAGAPRLSPFHRSLETISPIGPSTATNAPIRNRVLAAAAASGGGGSEADTTASTSHVLLPTPTAPPIPPTSSTSPHSRALGFLSTAVSVLAVGAYVYTVINRRQASGDATAGSRTIGQIPPAAPAPPVPPPPTKVIADSGLFRPANATGTAPVKAAVKTKANAAVLGSLPAPALAPAKTGVAGLAAAAAATAPRPAAVVVGPEQPTASPAREHGWWRRLARVYYISFGAQYGLVPRVRLDGTAGTLGSGAAAASAAGGGGGGGGGSGVVLAAFEDAADAEYVANSLWENLVHIGAQPSGPRPSTMGVLSAAPIFLEDLAAMRGAPVEVVPTDGLRKDVQLSTAQLEVVLAALVRGVSPEAAAAQAERVLDEAAAAAAAVEAAEADASIQSASGAALGRGGGGSVATATATSTSARGRAGTTVRNPLERTAEERGVKIRLPSKFDFPGFDAERALEPTAMPVQQRVPAAAGATVEAAAAASAAALADCPPPQSTDTSSATPLPPGGAPTAATTTTLPQVTPSAVQEPTLGVHGSDVEGDLQYDVWTPYGGGKVVDTEAVDVSATAAAMVPPPSPPVSGAVGAAATAVPSTAVPSREMDEAMREYQELETLLMMATNTRRGGAAAAAVTDRRGEFRQVAAADRRNDASSSILTAPAPSAAANLSPSVSEEGNQWLQPSQQLQSQQREEGAGAEEQGERKSRPERAAEIIAQKGPTDAALEAALAALGDVDDERTEENLFDEYFSIFNSSSSDRQEPDSTSSSSGSRNGRLQGEAGRAGSSSDLAAEVAEVQWQLAGVVQAVAAAHASGSPPPADATTTMQRLQQQLQNLQGRLAAVPPAATTLASVSSTTATPRSTAAATAAATASSRQSGGLFSAPPSLKATGKDVGAGGGRGGSNKNGNAGFKDAAASAVQAATAMNLDPSTLVTPLGLAGGGSTAARSDVRRAWGAWKTNRRIQEAESTLLKAATRAATLPSAEPPLPAPPRAAPSAPPATAAGNGDGGGSSGGPGADSEVVVVLSKRVGSNRPPKVVEAPEARQDRVKEALRVFSEMGNDVGDDDEEDEQLFGRSASLLAGGRKPWWQQRFQALFLPSIGYPDGSMGFLQVNVSLSGDRRDMRVLAFEDRSDCTACLTLMAQWPEHEGTEPRMSMMPTEKLLQQLQDIYDEQPDVNSGVSRPRGLAVFRRAKLPMRPGMGPEEFIQAVVYQHAAQVALARTGYGFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMN9</accession>
    <name>A0ABQ5SMN9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_015611</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040052">
        <property type="entry name" value="RBM17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13288:SF8">
        <property type="entry name" value="SPLICING FACTOR 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13288">
        <property type="entry name" value="SPLICING FACTOR 45 SPF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031066">
        <property type="entry name" value="Splicing_factor_SPF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="1" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="G-patch" evidence="5">
        <location>
            <begin position="278"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="355"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="130"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="198"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="152"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR031066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="47182" checksum="48B9DCF007799B89" modified="2025-10-08" version="1">MSLYGDLPQAKNSSIDAGGVASGWSALDSKLMPPPSLKKPAVPAFKPPPSVLRAAAQAGTGSGPGRGGPARGIPITGRSGGRGPPPIIAESTSAGPVASTGDGTSTPGSRGTAPGGFTAAAAVTVAGTVSSSANVSGPSGPSAASSSIFARSDGRPIEDEYDPMRPNDYEEVIAARERARKAAEAEAERLAKLKEMEAEAERLRKQEEERAHSAAAAGSSGTSAGGVMSMEEYERRRAAMGMTGDDAFARRGRLAPGPSGAGGAPPGDEGGGAGGNKGMSLAQKLLEKMGWREGEGLGKNRQGISNPLVAQKTNQRAAVIVEAPPAPNKPGTGADKEGPDAKRLKGIVLTGAPSRVICMRNMVGPGQVDEELEEEVGQELTKYGKVLDVLIFEVTTPGYPEEEAVRIFVQFEKQESATKSAVDLQGRFFAGRQVRVSFFPEDRFLTTDLAPKLGEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNT9</accession>
    <name>A0ABQ5SNT9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016659</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46620">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN SPF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46620:SF1">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN SPF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HRDC" evidence="3">
        <location>
            <begin position="772"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="1354"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="687"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="903"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="996"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1165"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="759"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1412" mass="143267" checksum="6B4AC873DABA59A5" modified="2025-10-08" version="1">MYLAASPDAWDLLWTFPDVSVASTSLSVPSKSVVVHRVAPTDPIPPHVNASKCRPFGTSKPIARQLVGAFTYDAVTPEAIVLGCGGSAKPSDAPGHREVDAGHKTSQSVKRCVQIADENDGKGQESRGCFLDTVTSSLHCPDISGDVSGDAASAPTSSVNSLTSRSSVQSVTTLDGGGDGRRVGAQDANPTEGETALVAGDRQHKKSVSADSRHHHFLPGDHVAGERIREPLPSTELSDGSETSSSSRSAKISIGACTGTGTYSTGRVGLGSCQAADAATLAARNEMGRWISLDVITDGRGVHSTAMYGNGDPIDRGQQPCHTASEEPCIAVTMEGPGVTAAAAAATWGSESLRQFYRNRDPRRQRSLECQQQPGLLDVSEQQRQQYRHQFISKTHIPTAVSLPPGGELRSLFDASLRAPSDAMPPLAGVPAEAASGAAAGKTSLSATNATAAAADPVTTYTGATCDAAMSPPLQHQQRLPLQHTAIRRGPRTTTALLLSKVRSGVAAAAAPAVVAAARVDRDFGACEAVCVRGSEWWPRRTTVGCCKQRVGVGGGSTRRAAEGIRKNRSTHLQPGLVVYDRDMEDRLEFEASGFSSDSSSGGWGGLRCATAAAVTAKPHDRRRTATSMLDEVQRALAAAAANADTDVAVTAAVGGGLHHRKAWRLPPLLRGRFDFSTARRRGSHVAAVARGGRRGLEATEKLAEKGGGPLSRPKAMGSDLERLRGAGNGAVGGAAAACPSAGHTLAQPPGSGSRSSSSGGGGGGDDGGNGSSPALALLQALDAWRTRRARSVRKTPEALLRLGLLADLAARCPTAVLELEDFPGLPRPFTRKYGQEVLDICRNHAHLRASRAAAVGATGGGDIGGGPRTYVSSGGGGGGGGGSMVATAAAQPVFGQRLAEEGADAIDRAPRPSSKCSDDGGDDDDPVGGGEILGAHVNDAAQGCGESGDDSGGGAAEDGPVCVATINRPVGPSGDVAQHVWRMRQQLLHILGVQEGDDDRPPRATMSRRRLAPSSAAVGGGGMVGSDVCGAVGGGGSSAPASGDLTPRGRCGNADTGPLSTGGAIGSGNRGGWEVASSHMCHTSGCRAASPSPSPSHPSDEIRKVVSTAAANGDVGGAGIAEGVGPKREAREVKAAAAAAKSTAGASAAGTADAAAATRCAGKRLTPDGGGVHRSSPGKARKCGPADSPRQAATAAGMVYLGALRAATAGEAVPAGWSPLPEGCRARSGRRLALASTPSLNGAKVKCLLGYLAEAGTAAAPLLEAIVPHPVLWGSDAGGTSAPSSSAATVTGASASAAVVPAAAVAMATAAHRLGRIGGQEQLRQLRGLVEVAMPAWEVRRVLSCRTLSPAAQAVAVLRLDYGASRDTVRLAFRRLSLLVHPDKNRSSGAADAFALVSAAASRLLGGQQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPV6</accession>
    <name>A0ABQ5SPV6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016534</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="873"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="803"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1210"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="842"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="872"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1223"/>
            <end position="1234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1249" mass="127595" checksum="D9A5812415038007" modified="2025-10-08" version="1">MFLNLISRLCCGVPSENVVAGPALEVRGGGKQNRASNDNDQGLQSAGYPCLSTPEDVAVHKLVTTSQTAWTTEAERILQQELSSFIGQNATASSSAFHDDLPKTNLTPTVSTMESRGQAPSPMTEVLADKDPNLRTKEPARPQYPAVPSSNKPHSPFTVTSVPATPLDGPQLQGGSLFTQAPPAGLTNLLCGAASGMDLRTRHVASIASTEMQLTTSLSEPLIHSISPGNGNQEQLNCSSSIGTAVTATSGGSSAAAALQQHPVWTGTATGLATSAYTASSATAISVAAVPVAERRGCQISVERFGNRENRSRRGSGTVERSGGSRLGRPELAVLGAVASAATATATATAAATAAATASATSRAAAGADVRGPEPPAGGAEGAAAASKAGVSNVVMSGDAGAIVNLDTGGGTTKGSQTSVGTVGGSGGGGVRGGSALGLLGDVTRRLLHRVHDVSIGVSHAAAAVASRADGAYGGVAAAPDASASGQGGGGGGSESASAAEVREDGAAAPVQWTTAALAATPPNPLPQVPPLPCSAHGQEHEGQQLQQEKGQERSAANSQWIRPRTEYGQTEAAATVTAAAVPAVLAAASPPAATALHMPYVAGRGSLRGGSDSGSAVLQRGQDGFRVVQLSGAYPPYAGTGPWTSRLTKYADRDVSQVALMQMLHFGCTSGRVPLQHLATLNLAALSREIRALHWVGQGGGGAVFQGVWQGAPVAVKFMLATRPEHVDATALEAIVSLAVGHPNVVTTYSFDVTRVSEASLLPDAAAAVRYSRLSQQPATLGEDSSATALFDALMETDTISEPPGLAGCGGAHRHRQHGTPGCSGSGSSGSRPTSVHRTESLSVRLRRAQAAAAVAGNTPDGGAEGTPIVTATAADGSSDGSLGSNHDAGRAGAAGGDWRSDAARAPSIRPSQRQHAAPPPLETLGIESAFSSEEGFGDPDLTDNSPSWTVRHVLAYLKARPGMYMTHIIMEYCDRGSLLSAIKSGIFRMDGLPDSDVVAASSGAAAASGAGGAAATSTAAAAVGLPALPRFTRRVVLRALLRTARDIAKGMCHLHANGIIHGDLKPGNVLLRGCRSDRRGFVAVVSDFGLSKITRGEKPLELNHWSTVTVMAPEVIMGRWLKASDVFSFGILLWQLVTGEMMPYGNATVHQVLMGVAHGALKPEWPASAHPPLVRLGRACLATSPEKRPSFGAIVKILIKMEQHVRNAHRPTRDLDSSTEGGSQHSRSSRGSENAGKFLNRLRSSAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLV9</accession>
    <name>A0ABQ5RLV9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EngB-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_000323</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03598">
        <property type="entry name" value="GTPase_YsxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11649:SF13">
        <property type="entry name" value="ENGB-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11649">
        <property type="entry name" value="MSS1/TRME-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0717">Septation</keyword>
    <feature type="domain" description="EngB-type G" evidence="11">
        <location>
            <begin position="227"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI58595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="421" mass="44886" checksum="61464A98D355808B" modified="2025-10-08" version="1">MVVLAALPPRSGKSAVQSSKRSSPNVKGRLREAKRKSEKALIQPSKPKGSSTTSSSGVGPAATLAPAAASPRLPRPVASIGHFTSSLEADTQRKALDSVAIGPAALAAGASASLGELIRGTAPVDHVAPGDLASKTSGEDFAASEPGQDKDSNVEEDGFLVVDGDVSEIGCQDPCQDDVQADAGDMATEEDAFPEIQFNLPNPTNVKVKTAEYLQSCVKVPDCPAPRFPEFAVIGRSNVGKSSLINMLTGRKDLALVSKQPGKTKCINHFLINGSWYFVDLPGYGYAKVGAQGRQQFDAFTREYFKMRPNLAMVFLLVDASIPPQKVDLEYANWLTDNEVPFTLVFTKADKRRKGVPSSARDSHVTSFKRSLLKDLAFLPPTILTSAAKGLGRGELLNFIAGLRVAFERSGRLTAIRQGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM03</accession>
    <name>A0ABQ5RM03_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP 3',8-cyclase</fullName>
            <ecNumber evidence="3">4.1.99.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_000348</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21117">
        <property type="entry name" value="Twitch_MoaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_B">
        <property type="entry name" value="MoaA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013483">
        <property type="entry name" value="MoaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02666">
        <property type="entry name" value="moaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="15">
        <location>
            <begin position="313"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="173"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="239"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI58614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="638" mass="68660" checksum="A4094D995A7E32CE" modified="2025-10-08" version="1">MTHRGFVYNLARYAIGRSRARSAKRGHVGNYPSEASEPSTSFSCTTSATIWHRCYYRSTGARGFCSWSLTATVTSQPKCQEGAASAHWVLDPASSEAYDRRLRELVNSSRALQDLPQDERQRVTSPFARSEVPVGAVTYGVGDDTSAIQSMLAAVNVGERKPTVAATAIAHGTASDSSSSLGLAPSNGAPAAVSPPPSVFPPAQEAPLSMQAASAARDRARAALRDVPAFTPPQAGGCASTSSRRSGVKSNTAAATSQPMPSSVATTATALSEPSAQPHPASPTPVDWREALQLAKRMLGGRQVSGMEMLTDKFSRMHTYLRISLTERCNLRCTYCMPDEGVALTPPQQLLTTQEILKLARIFVEAGVNKIRLTGGEPTLRRDVCELVRHLSALKPMGLRGVAITSNGIVLARQLPALKDAGLTAVNISLDTLRPERFEQLARRPGHRRVLESIRTAVALGYDPVKVNVVVMRGVNDDEVADFAALTRNQPINVRFIEYMPFDGNVWSDSKMVPYRELVSRIQTALPDNPLERLSDPTGEVAKNFRLTGHRGSVSFITSMTQQFCSDCNRLRLLADGNLKVCLFGASEVSLRDAMRGGASDDDLRAIISVAVGRKRAAHAGMFELAASANRPMITIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM31</accession>
    <name>A0ABQ5RM31_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="13998" checksum="D9E4D8941FC4F45A" modified="2025-10-08" version="1" fragment="single">LTYLVTRKVVADLRAAVPLLLQLLINLASGPLEGLQVLVRLGAAAAVKQLMRWEDMKAKAAHFLRLLDELDLEYKPFGDWGNGPLTAAALSALQLDPKPFVAQAISSSKLLKLRDHDLRTRFGLDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM65</accession>
    <name>A0ABQ5RM65_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000418</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000554">
        <property type="entry name" value="Ribosomal_eS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047861">
        <property type="entry name" value="Ribosomal_eS7_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01251">
        <property type="entry name" value="Ribosomal_S7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00948">
        <property type="entry name" value="RIBOSOMAL_S7E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="194" mass="22186" checksum="53C77960B4B043DC" modified="2025-10-08" version="1">MVSARSKKIVKERGQEPDEFEEQVAQALFDLEATNAELKSDLRDLYITSAKEVDVANGARKAIIIHVPYRLLKAFHKIQQRLVRELEKKFSGRDVVIVANRRILPPPRNGKTNARPRSRTLTAVHDAILDDLVYPTEIVGKRIRYRLDGSKVLKVHLDPKDRNTTEYKLETFASVYKRLTGKDVSFEFPVAESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMU9</accession>
    <name>A0ABQ5RMU9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="21286" checksum="683B48E24676B957" modified="2025-10-08" version="1">MSVLARFMGNPSKVHMGLALGVLRYLAGTKELGLCYDGDGDLAMVGYSDSDWAGDPATRRSTTGYVFLLNGAAISWNSQLQRTAAASTVEAEYQATAAAVREALWLRKLTRDLGLGTQAIQIRTDSEGSMSLGNNPIMSVRSKHIDVQHHLVRERVLRKEVALSHCSTEDMVADVLTKALGEIKFKKCITVMGLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMW8</accession>
    <name>A0ABQ5RMW8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar protein 56</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000287</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="7">
        <location>
            <begin position="294"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="433"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="445"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI58543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="504" mass="56419" checksum="E94B3FCF9C556466" modified="2025-10-08" version="1">MATFVLYESAVGYGLFEVIEFDEIGQAVDKVQEAVSDLARFGRIVKLVAFKPFPSAADSLEQINAISEAQLTEELKNFLELNLPKVKAAKKAKFKLGVFEPKLGSIIQETTNVPCISNDAVGEVIRGIRANISRFITVSDADLKRAQLGLAHSYSRAKVKFNVNKVDNMIIQAIALLDTLDKDINTFVMRVREWYSWHFPELVKIVNDNYQYSRLALVIKDKSSLTEEQLPAMAEIVGEEAKAKEILDAARSSMGQDISPIDLLNIEVFAQRVIKLAEYRQKLHTYLLDKMHAVAPNLSALIGEVVGARLISHAGSLTNLAKYPASTVQILGAEKALFRALKTRGNTPKYGLIFHSSFIGRAKQRNKGRISRYLANKCSIASRIDCFMDASTNVFGEKMREQVEERLRFYEEGVAPRKNSNVMGEAMEVLKTHLVTEEKEDGADKKKKKDKKEKRKRDEEKEAADPVAVPEDDGKKEKKKKKAVVAEEPAVEDGGKKKKKKVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNR2</accession>
    <name>A0ABQ5RNR2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009522">
        <property type="term" value="C:photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.40">
        <property type="entry name" value="Chlorophyll a-b binding protein like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000549">
        <property type="entry name" value="PSI_PsaG/PsaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023618">
        <property type="entry name" value="PSI_PsaG/PsaK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016370">
        <property type="entry name" value="PSI_PsaG/PsaK_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017493">
        <property type="entry name" value="PSI_PsaK_pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03050">
        <property type="entry name" value="PS_I_psaK_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34195:SF2">
        <property type="entry name" value="PHOTOSYSTEM I REACTION CENTER SUBUNIT PSAK, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34195">
        <property type="entry name" value="PHOTOSYSTEM I REACTION CENTER SUBUNIT V, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01241">
        <property type="entry name" value="PSI_PSAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01026">
        <property type="entry name" value="PHOTOSYSTEM_I_PSAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="115" mass="11198" checksum="1F4D398A39747E11" modified="2026-01-28" version="1">MALAVRANAASVRPAKATRRNSVVVRADGGFIGSSTNLIMVASTGACLAAARFGLAPSVKKQATAGLKLVEGKNAAGVISNDPAGFTIVDVLAMGSAGHAIGAGIVLGLKGIGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPJ8</accession>
    <name>A0ABQ5RPJ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000759</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045278976" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="43"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="278" mass="28949" checksum="05143F26E71C8246" modified="2025-10-08" version="1">MAGQSYLPVFLVAALALGWAFSAHAVDDDFVESRDQLGSIPDFPFRSCNKTNGAYRLAPVWRPLGGGKYCFNIEVRQDAQSCSGACCTAGLHKIEFNVSSSCLVAGSSVTATINGVPTRVGAAYDIPPSGLPGSAILRITQLGLDPVTAQGAELCITLKPNRARQGCTTLEQMCASPGFPAGTCTAATFDVACNCCPVSQAVQAQPPPPPPPPPPPPPSPPPPPPPSPPPPPSPLPPSPPPPDVIAIRHCNVCVTAMLMPPAYDVWPYRFDNTTCAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPL2</accession>
    <name>A0ABQ5RPL2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AMMECR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001397</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="2">
        <location>
            <begin position="34"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="259" mass="28419" checksum="0CBC498B22EEDB04" modified="2025-10-08" version="1">MFSRCFLSRIEKVNGSKPQGVGTGAMVLPTSADDPQDPNIATRDHCIFAFAVLHSHLTGNPSPVPSFPDSRCALFVTWNALSGGGHWRLRGCIGTLESKQLQRALHDYALNSSLRDHRFSPIRLKELPSLQCKVSLLARFEKAAGWQDWTVGIHGIIIHFTDPDPEARGARRTATFLPDVAPEQGWNQQQAVDALIRKAGYNGPIGASLREALSLERYQSTIASITYEEFMEALGQAEEQRGNVLGSSSPRDARLQVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPU2</accession>
    <name>A0ABQ5RPU2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia-and flagella-associated protein 96</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001458</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CFAP96 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029358">
        <property type="entry name" value="CFAP96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31144">
        <property type="entry name" value="UPF0602 PROTEIN C4ORF47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31144:SF1">
        <property type="entry name" value="UPF0602 PROTEIN C4ORF47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15239">
        <property type="entry name" value="CFAP96-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="181"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="254"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="335" mass="36994" checksum="4B91804A5D0398A6" modified="2025-10-08" version="1">MTTKYGMFKDHIYLTPADPPQEGSGSQSGSRKTSRSSSPVGTQVRDHGLNFKATIGRTGKGNDATFEPFKGLYNGDKYLDAGRRRLQEDNVQRKQQVATAPFKAASPMKISTTPGDFVGTLQGKVEYVPGRSVTLQKTKADVHNEPRGFYTSPAKKGTYGFNKTTLSERQGFKGVATEYEYQHDPDEMQRQRRLQEREADMAARQPQPFRPSNPSKRGGAGVPNVTISKGRGIAGEYEYVLGADPAVAPINPEAAKQAATEPTEDSGPAFRPSSINPRHLGRHPEYIHDPELPKLDAEHARKKAEQQRLASTGAWRPNNAQKTDMIRSIVRMNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPV6</accession>
    <name>A0ABQ5RPV6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="3">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">VaNZ11_001524</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044912">
        <property type="entry name" value="Egfr_JX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000:SF679">
        <property type="entry name" value="OS01G0170300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045083020" description="receptor protein-tyrosine kinase" evidence="17">
        <location>
            <begin position="31"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="755"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="660"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="661"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="686"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="702"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="718"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GLI59595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="834" mass="88995" checksum="EAFDDE83D4840148" modified="2025-10-08" version="1">MQITIELSHFRYIVSIFVFFGISLTQPAAAQSDLETNVLDFCGNWTFGTANACEFYDTQSIPYLEDRRFYFPIPPGASYDLIVTVWIYSGDVDAELLAPNGTELSSSSERIGNEVVTATRAVLSRNPGNYTLVLRAVAQAMSTYGMMIWTPNTEVVTRLALEDKAALSSIASDCCPNDDDILPDSCTTLLLATARDATDEDDICRKPPNTCDENGHLTKLFLSASDSGNFVCSSFPISLGQLSKLKVLDWTGNNQIGTLAEVAKVLSPLNSTLERLYLGRNALSGPLGCELVNVGRLEILKLEGNMLNGTVPSCLLKSPTLKQLSLASNLLEGTIPSLSDCKLVMLDLSANGPTLGVPTSKRGLEGTLPSLVQAVNLAYLNVQSNSLIGTIPTLPPQLQVLQAAYNHLTGSLRLDGVPDLRVLDLSYNELIGTLPPSVASSKLLTVLRVTGNQLSGVLPNGNWGSYLMEVDLSANNFTGQDLGFLTKPSLIKLNLASNNFSFDIKALASKLPENNSMLYFNISHNSIYGQLTEQLGRMQLFKKLMQNALEDDETLQISVPPQPLFDISYNQINSTFPLKLLPLLGNLYGDTPDADTPYIPLSFVLQPQTPPNFISCPSGDTEFLTAVIYLSDLEQLFDLTCMTQADQRVNISAFQTVFLSPAPPKPRLPPRPPLPVPPRPPIPGRSANSTSSSGSVPTPAQGLPSGSASSRGPGGNSDVSVGKSTVGELSSPPSAGAAADAAGSSGGGGGGSKGAIAGGVVGGLLLAIAVAVITLYFVRRNRTHRGARASRRTPLTFNSAYEAPSEATASNYGDMQMFEFNPLGRDPANRSRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQF3</accession>
    <name>A0ABQ5RQF3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="343" mass="35088" checksum="786C9EB29ADF6863" modified="2025-10-08" version="1" fragment="single">MTCTATIIRSGHAMPPGRVSVSRRLGQAQATIRRGMQDVGGILVGDDNRLYGAVFPGFPLGVSRIRPYSVTSAFPLLCGSMSMSGHNLHASLHASVHATANSAGASKAAAAQSGSVSRASVVCHFLPVENGSCPPSPPRRKGGLSSTSGDDGDKGKHPFASQGWLQHVQPLMVGVTICVGIGLILFICLPTVGRELGVDSTKVAFPEGSLGLPGAAAAFGRDAAYKAADTSVIITDKVCNTLLSIADMASTIPDKTSTSADKTSTIADKAADTSVIITDKVCSTLLSIADTASTIADKTTYTVDTIAEKASNTVINSIKVIVGGVVVYKVIDVFFKGGGSGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQK4</accession>
    <name>A0ABQ5RQK4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CFA20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001823</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040441">
        <property type="entry name" value="CFA20/CFAP20DC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007714">
        <property type="entry name" value="CFA20_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12458">
        <property type="entry name" value="ORF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05018">
        <property type="entry name" value="CFA20_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFA20" evidence="2">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="499"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="818"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="869"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1217"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1291"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="413"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="676"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="875"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="898"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="937"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="964"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1052"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1062"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1107"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1134"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1226"/>
            <end position="1238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1471" mass="141218" checksum="5A38A680890B9E7E" modified="2025-10-08" version="1">MFANEYQGGSHVEVLGTQGQNPLAQWKVSGPQKGVQKVYDKALKGYVFVSSGACRMVLPKDDRSSLGLVQPHLLLQLEVGVGTPFSVEVGLSDASATRRRVILSSSFSELKCTPLHCQVPFTCLPRESWLNLVLPIAELAAVLFKGGAGGAAAAAAGSNAAALAGAASGGGSFSPSGAATAALNAAFRTLDSLQVAGTCRLRRICTLREAPQPPESQDAGTATASAASAKPLEFPAGMDHQTLLLDPRSLTLVGINGAGGGSPNQTMTLGGSGASGAGLGIVGTSMGSSRRPANGGATSPLPGVISRSCPTPGSKGGSGGSAATAPALNGISWEEDGSAGIATQQGPGALAVPNLHTGDTPTRGSSSGASTPTARAGSGVPTLPPAFGAAPGPIHHGPGSSDHIATPSRLGRSTSGYLNNINNNGSGSPPPPPQTGPVSGPASGPGSTSGSGPLPSLDLNTSLSAVATVGGGASGGGGGMKLPRLQPNGGGSAAAAPVAAAAAPSLQSSGGAGPDACVSGEDASRGDTSPFGSRRALSAQTPPRQSGSATPPGSAGGTGATATGGGGGGGGGGGGKPPASPWSGSASSKRAQSGPARISTEALAQGLGETVAPTDTPMSPSLAPRQNRRTQNNNQIPPTISENEVVNPSSTANGAHAIKSAVRSPSRVRPQPANPAAVSGAHTVATASATASTANSSGLRSGGGSGNIEPPSPALPQLRHIRAEAAAAAAGATAAAAAAREPPASPAGFAVIASNANLSSQLSSVLTIRGGVDASIDFGAHSHLLEGSPGSSAILSSNLRISSVSGHTHRYGAAAATAAAAAPPGVSTDALSDSDADSGMPSPSGAAANAGMGSSVVTADAVAAKASRMRTRVLPGGGGGTAGGGGKGTTAPDGASGRSLSTPASVSNSPGLITRKVTGHGSDVETPTRAGRGGLSSAVSQNGQNSPANMPSRSGGSRNTPLPSGGGGGGAGAGIGLSNLGPSANGGRARKEDGTISGRPSAAAAAAAATMTTAGATAVVSGGGGGVAASAGGGRSHAAGSSGGGASAAGKQPSSGTGSVCSSPARQRPAPPSSATRGSTKPSGASGGGATPSEHRDKLPPLSAAAGGRGRGTGANGGRGGGGGAAAAATASTGGNGGRGNPAGGRSICNRRPTTSNEVDVEESITLNAGLWGGFGVDEDDDMLGSRHFRNGACASSGSDGELGGGCGGNAPSLRRGVGVGGGGSRSSLIHRASRSSSPDLPGDCGGTTEDLPSPPNHYANATVNGLLSPPIRRSDAASALTSQDVAPAFAGFPAPTSRRSSSAAGGGGNDLRPNSMRHVLDSLLDEEALLAGHVRDLDHYDDWAKVDQRPTPPDLLHSHRAFTPPVVPASKALGVAIEHSRGGSGRVAGGGNGGSGDGAATAIIAPVTDGDAQVLRVSAAAADVAAGRAGARGPGVVVGGGGEAMLDLVYDPILNCYYDGATGRYYELKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQP0</accession>
    <name>A0ABQ5RQP0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_001426</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
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            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
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                <person name="Mahakham W."/>
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                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="889" mass="92046" checksum="FDAC95D2E6FB3FFF" modified="2025-10-08" version="1">MYTLHVVASERHLSQISLPNAVGGGSEGGQRGTGSRSQGLLGEGDSFSDDSEEELLGLDLEDDNQIIEFTAGNPRVEHITGVVHLYRPTPGRLPQTTSSAATPTVSALDAPGQLHQTQRGGSSVGSALPALINAPPPAKGHASWASLLGAPAPLPTTQNAPAAGDVAPPRAPFAGFGCNELCCLALPSDMTIADFCTFLGAHLLRLRRMRVLRRQVGATTHVVAALAATAMPTSTLAAALPLPDPPAARSSVGVLTSTSRSASGGGSGGGSNSSALLSVGPARQCQEAQHGPAATPRDEQPMTDTVSESERQCRDGDGESSSYGLPSSHMPQAQHLQFQQQRQQQQQQQLAAQGAASRRPPHTTRPPSQRVVCMVLMHMENPEAADELYNDLNGKPFSSLEPDIVCRLVHVRHVEVTSGGGKHAAAADGSGVASAATASVDGTAAAGDLAAALHVPPPGQTELPTCPVCLERLDEHVSGIVTTVCNHMFHSECLQKWADTTCPVCRYCVQGVANTSRCSMCSTAVDLWICLICGHVGCGRYRAGHAADHWCTSGHCYALELETQRVWDYVGDNYVHRLIQSKTDGKLVELAAPATAVASPHAPGHGAGGGHRHHRYHEADHASCGAAAGGGGGGDRSAGQRGGGGGAGMGFGGSGAGVGFGGSVSGGAGGSGGPDGGGPGWSTADEQLKEALMASKLDAIAVEYNHLLASQLDSQRQYYEGLVAQATLDADHRCAQAGAAADKARAEAEEAQAAAREEGRRRHAAERRATETAEALRTVRQEAEFLRSLNETLLANQRDFKQQLAAEKARGDAAEAAVKELQEQVRDLAFFIEAQRTINDADGGELKEGTVLPLPQSNSRAGRASTSSSRAGNRSGGAGSGNRSGGARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRK8</accession>
    <name>A0ABQ5RRK8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="485"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="59473" checksum="ECE4DCAC49FB5478" modified="2025-10-08" version="1">MMLKDSSIAPDRVGTTFASRHRFGVHRPNSHVSRQARMLIVRMGRNDGASGNRRRSSSNRNRQKPEISDPINGNDLWYTDAPDLGPSSNNNGSTQQQQQQQHQQQGASSSQFIEHSGNLSKHGGVRPSRPRRGRGLQEEEEEEEDARGWWERPEERSLQAYAAPSLEAWQEERLQRAYASGRRKAQIQDLARELDVDRSVVIGWFKEFSLKPQSEREAILAAFRGERMEAAPSPSSNTDLADGAWRRGAASGAGMSAASRPDSKSSSSPSTASPSSSSSGPAAAPTGFVPFYLRKQQGQDKQRRLRGEVLRTLEAVYDRTPFPSADVLRGLFELHRVPREVATEWFAARRAEDGITSSTHKRPGRLSLREKDRDLQGAFSGGGDEDDGRGDISSVAPSSSSLLSELGAAQQPHEEPTVVPLSRREMAALRSSLPSPTKHKGRQMADKLGIKTGPGAASTQIGNVQYIEEAVAAPDTVDRVRASWRFRASSTRTAAADGGDEETDGSAPPPPSGGGRRLRLRGRERGPRRRGASGGGAVEYEEGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRW4</accession>
    <name>A0ABQ5RRW4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001729</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="253" mass="26902" checksum="45F4354527F4B8E0" modified="2025-10-08" version="1" fragment="single">MSLLIDRISHESRTLDLQSRPPRGLSVLGLRLVCVTRNETRRFICDRKSNDDRLRSTLKIVRKIMTCTATIIRSGHAMPPGRVSVSRRLGQAQATIRRGMQDVGGILVGDDNRLYGAVFPGFPLGVSRIRPYSVTSAFPLLCGSMSTSGHNLHASLHASVHATANSAGASKAAAAQSGSVSRASVVCHFLPVENGSGPPSPPRRKGGLSSTSGDDGDKGKHPFASQGWLQHVQSLMVGVTICVGIGLILFICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRZ7</accession>
    <name>A0ABQ5RRZ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_002436</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1217"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1504"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1513"/>
            <end position="1523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1524"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1538"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="1244"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1679" mass="172636" checksum="4DE6A69F266C4215" modified="2025-10-08" version="1">MRIFTCCFSANSGSQEVSQHKVTSPPSGLGTVLSAPFEADNIVSTTVHSKAPYLNIRELCTEKEWKDLNCNNAKDASGGLECALSLQAKLCAAGRTNQFREARAGYLVDIVQKHLGASAARLYIMASGYSNDGAGRVVECPLVHLACALSRGTAAGARSCNAQLGQDCSSHSEYLCPKHEPVGSSSVLLRQAMVSKVSVVLKCPARQEPPINVDSNTPPSLGGQSRVFANPAGGVSVGVKSTHMGPAAPAVAGAQSNSSGYSSSAVHAELDTAAAAAAKCDAQCCPDLDLMETDALPGSAMVFAAMPLVYGMATLGVLWLAVPIPDEDTPNGGSPTYQLQTGTSTLTGSDLGCSSPPFPPLPPTLLQQQQEACNSSRYGIGATPRVVAKGSSLSLAASNAPAALLSNPAALEHVALSVAMHLAGGCADVAYLCRLGQALHGLAESATLAELVLELCNSLTQHVRQQFYVDVTAHAALVPAAESRVAFMLRSERVEGTASVRAARQQLTQSRLRMSSVASVPSGTNLLNLSAVAEANRSRSVTLTAHQMANNSRRVNPAATAIAAANASAHSKPQQEHQLQRAYTTTTASGDNVNTGAPRACSLSALRPSVPRDVFMLESSVMRSDGSFGLLSSCQAGAGGTAGVGFPPPALSARAFRLSNTLLQAMLHAAQVQQQDKHRSGGDVVSGVVSGQGSRFLIGVDRRKLVPNSFDDSRCAAASAVGTTSNDGVLAGVCVRDCARHVQDERQPSRDVCMLMMSAGDGTRFAAGTPAGLSSSVRGGGAMLNSEPAAVVSAAGGNGGGGAQSLVLLGIETGDGAVLAVYLAFPTPAPIQLLDAVREGCNALLREHLATMVRHRLRCADLAAEWETLRAGVPGSYLSVSRGSIVPPDVSSTGTAMSCIQSPLSQPSALRAAPLLMMSAGSNTMAVATTDDGGFLQGALSSCRGIASLQGRGVLGSVPGTAPVVVAGSEASLVSLAPGMGHGGGQMGAPQSASNISANGHASLTSGVMGGAMGSGPMGRSRKPSLTHGGMEDAGLTLGGPAWLQRSRRSASAVPMVLAPARSASSRGLFMRMGSLKHAAGSGGGGATGGLLLQPPAGPTRSPLNSGCFAGRFECTNSPVGPLTVEADDEEHGGWVESMAPGTGSAGGGLAAAGMLTVHDLDETYESWGMRAHMGALVDCILSTTLRSSGAHLRMDDGGAGRGEMAAARVTEGLDELQLSAVLGQGASGVVLKGMLGCTPVAVKIMEMPDADASIRDGVNMSLHPSGDRGGQAYLHSSDDDTAICAGVGGGECSQPDLGTGGSRAAAEPRDVRARRALLRNAMEVAIMRSISHVNIVQAFGVYDNVIMERHEGRQGIISLRRINQKDANDQKVPDAMNPGTMRSSPICTAIVLELCDRGSLADVLHNRSFPALPLPPPHPSASPAATAGAAMQFARYWKGVFMTLLEIALALRHLHSRRLVHRDMKPGNILLKSSPGDPRGWTCKLADFGFALVLDQLDQPSLEENNPLAAPAGGGGVGGAGGHCSSSSSSHPLVSPLQTQANSNPPSAGTGTGTAAGAVGPRDWYTIQQHIGGTVTHMAPEAFKKGSRIDASVDVFSFGIIMWELVCSRGHRPHKDLALDAINNAVVSGARPIFPPGDHVPLSYRQLARSCWSVDPRLRPTAAELVTIIKGLLESTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT35</accession>
    <name>A0ABQ5RT35_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47759">
        <property type="entry name" value="OS04G0509100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47759:SF2">
        <property type="entry name" value="TRIGLYCERIDE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="166"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="604" mass="65718" checksum="1D07B2E4CA8540E1" modified="2025-10-08" version="1">MSALSQLSMIAVGAAGGPITMGLAAAGFAAAGFFAGFWSASTRQQGLGKAARFAHPREVVEETEPGEASQSSRTGINESPELLLEFLSDSPVVPAQVPTILMTDAKRGEGRSRYQRSAGIYREEKDLLPPGEFDIDKADYLLDLAQHAYKNPPPGQGYTTPGLEGPPAVTFFDKSILTAIYDGVLEVKVERATGLRPLRGKGDVSAYAEVTRGSSSFRTKAVRNTASPVWNATFTFFVGNTNWDSHVIKVFDNNNGNTELGTAGLGMGDLMAHPGRETKLWLPLTGFHGEAGTIYMTCRYTSFQETDPDDIPELKPITEKVASGNFSDMKPIGGLKPAAFVECKFTDTQVWLHSDVAQRKLVFSFRGTEDIRCIKDIWTDVSFVPCKFVPVIEDVPAGDPQISVHSGFMRAYRSVHMQLGALVRAITSSEPWDVEVTGHSLGGALANLAAYELAVYRTSAGHVKSVKLYTYGCPRVGNDRFAQGFNKVLRDAWRITNRLDLVPSLPPSGIFIKYTHAGNPVRLRPDGCFYKGVENLKKDIERDINWFGSIISTKGFKQHYGQFYKDMLTNAKNLLGTKASTSASTTEIPAKAADQAAKTIGSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT87</accession>
    <name>A0ABQ5RT87_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="147" mass="14977" checksum="7844DD111DD1AD5C" modified="2025-10-08" version="1">MGPHLPATADPNTPVAATPAATAPATAHEHTRPSDPGGGDARSPRGADAPSWDPWGGFWGAPPAEHPACDYDGRHGRRFVLLGGGDDGVLTPPTEYHSALGDGPGGSGPPTRSLRRGVRTSSGHTPSVVDAAIRFAADDIATQDMSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUI9</accession>
    <name>A0ABQ5RUI9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phosphatidylinositol 3-kinase</fullName>
            <ecNumber evidence="1">2.7.1.137</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_002884</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00870">
        <property type="entry name" value="PI3Ka_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00896">
        <property type="entry name" value="PI3Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057756">
        <property type="entry name" value="PI3-kinase_type3/VPS34_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008290">
        <property type="entry name" value="PI3K_Vps34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000587">
        <property type="entry name" value="PI3K_Vps34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="10">
        <location>
            <begin position="93"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="9">
        <location>
            <begin position="443"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="8">
        <location>
            <begin position="850"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="735"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="841"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="746"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="772"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="845"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI60682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1133" mass="121061" checksum="A95BE6CB44447BE8" modified="2025-10-08" version="1">MWGSAPPPRGAAAGTSRSAGQFSFYVSCDINLQVRVKVSELQGLLPSTRSHLAGPAGCLGLLADHTAGLGFDATWPHVSNSPAAAPGATAATGGSVIGISAAGSTEAGNGTKAGASVSNSTSALYVTAWLTSCGDTLGLEMETAYTRCHGGAARASATASGTANAGMKGAVGGGALEPLLGPRDGGAVRTPAPGSSAARGAEGDATARWNEWLTFCVKYRDLPRDAQLVFVVAEVTEGHGEALVGSSVMPLFSKRGRLKTGPQRLRVWEGVPPCTANPSSTPGKDPLAHRDRLGRLEHLLARLQRGEMAPGGDWLDSLTLGEIRRLSQLDREAWLTRPELRLTVVLPIFPYPVLSYQHVSYMAAVAAGPGTSGTGLAATSTASSKKDAAAALGALPSPDPSVSGGASGTGSIIRLMDPEVGRDNPAELKAQKLARGLQRGVLDRDLKPNSEERKKLQAILKLPPNKPLHAEERALLWRFRASLTSDTRALTKFLQCVDWSDAAEAKQAAELMRQWAPISAADALELLSPDFRAEVVRDHAVQALQNHDPDELSQYLLQLVQALRYEAGDGSRLARFLVARAAEDPALAIQLHWYLFCELDDPGFGQRARALHTSLQDTLERRAGHVGLAVKDTIRRQVDLVKQLKAVIRELKSSKIKAARATERLREMVSESGPCGELTALRVPLPLDPNIVLAGIVPKECAVFKSAMLPLRLSFRIEAAPFNWRSQPLAADGTGGLTRGLAAAHPHQHQHQHHLHQQHHMHLTRHHPPGGSPSMSSAAAASGGSGSGSGSGHHHSLHAPPADPLVLLPSRTGILPAPSGAGTGGPAAAVAAAIAASGGAGGASHHGHSFSHHGYGHPGEPGPLAQGGPAPILEGYRCWLIYKKGDDLRQDHFILQMISLMDRMLKRENLDLRLTPYKVLPTSCDDGLLEFVPSVPLSAVLAEHRTIHRFLALTQADPTGPFGLRAEAVETFVRSCAGYCVMTYILGVGDRHLDNLMLTNDGRLFHIDFGYILGNDPKPFPPPMKLCKEMIEAMGGQGSEYYKQFRMFACEAYNILRKRADLILSLFHLMAGASIEAIRGDPEKAILKLQDKFRLDFDDEAAVEWMQGLINESATALLPQLVETTHRWAQYWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUP0</accession>
    <name>A0ABQ5RUP0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fumarate lyase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005677">
        <property type="entry name" value="Fum_hydII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444">
        <property type="entry name" value="ASPARTATEAMMONIA/ARGININOSUCCINATE/ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444:SF1">
        <property type="entry name" value="FUMARATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="117" mass="12264" checksum="63397361F494A4DB" modified="2025-10-08" version="1" fragment="single">MGRAIMAAAAEVAEGRLMEHFPLLIWQAGSGTQTNMNANEVIARRATDILLAASTSAHQPPTSTSAPTASLNGAMSVHPNDHVNLGQSSNDTFPSAMHVAVALQVHHRLVPALHGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV14</accession>
    <name>A0ABQ5RV14_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="274" mass="30416" checksum="B4B08396B73170E3" modified="2025-10-08" version="1" fragment="single">MLVGRCLNTHSTTPASRSKTCSQRCCAFISSTGHQQAQIAQAAARTSATSEVCEGGIVAPVAGHLSGLYQLLEPPRTELSAQAQLNDRLKRCTTWEELAALLEADLDIVDFVNISTAWTRLAKLFGSTGLNKLNAQPSIGAPPVQDPAFRRFLVQLINTTSQQLRLFGVQALANVMWALSNADWKLEQYEPAVDKLMHEWCASVHDRLPELRPPDVSNISTAIGRLDHRWHPGFVIEFYRSVLQRQDLLERMDVTTLNNLLYGMSRAGHRGLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVB7</accession>
    <name>A0ABQ5RVB7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_003273</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16147">
        <property type="entry name" value="G6S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF8">
        <property type="entry name" value="SD21168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="6">
        <location>
            <begin position="102"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="473"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="589"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="812"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="481"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="564"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="624"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="637"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="649"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="715"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="817"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="997" mass="107666" checksum="F3E70FC2381BE940" modified="2025-10-08" version="1">MRRSSDKCSNYKHGRGKAENRGPCATAALGGGARGSATATVILLLLQLFALDTMAVKPANAKTPLASPFSTRASRRPTVPRPAPLPQPTPLPLVSRNGGSAPNFVVILTDDQDEVLNSTHPAYMPALNYLLGARGTRFANTLVSTSVCCPARVSLLTGRLAHCSNVTANWAPMGAWLKFSRLGLDSDWLPGWLQKAGYQTALVGKLLNGFSGLPYSCPHGFDHLDALTDGTYNFYNSTFSLNCATSHTMSGLYQTDIIRDKALSYLEAAAASRAPFYLQLTPAAPHVDNGGGNGWRPPPPAARHVDLYPDVDLPPNPTFLKANPLNPSRKLDMGNRDNVKAMTDLYVGRLRALRAVDEMVAAIVYKLLDLGILDNTYIVYMSDNGYHMGQHGLDDGKGTPLEEDSRVPLFLFGPGVAAGMVSTYQANLVDLAPTVLALAGLPPRLDLDGLPLPLEGLTIDAYATAMHQAATAAARRAGLRPPSPPLMPPMPPAPPWQPDTARPPRNRTRVLSETNISRRPLFPLVGQSPHPPLPGIPVRPLHEQQLTSPQSPSPLHLPLSDPSCPQSPSASANQLSLEHQRSLVARWSLGLPQPQPPQPPFRRQRPPPRAPPPRPRSASLPSKPRSPIPPPFPQPSHNLQVLSMQTPSPYKPPLSLSPPPYPLHSPPPPLPPRPPSSLRRPDPLPPPPVLIQSSSPSPVPAQLTGWQRPTLPSTITPPLPSAVPLPLPSSLPSPIPSPPLLSPPPTPRPTPHSLPLQQSQPPAALWLREAVILEAWDSDAKNGFFPGITFKGLRLCSTYQYNLRVLRSTPSAGSGLPMQQRSSSSPPQPLSHQQSGFDGLPALMVPQLDNRNPLYCLKYVVWCKGNQRELYDLTYDPYEETNVLSSAPAALLDRLNAVLSVLVHCRGADCSHPYAVLHPAGEVVTFAQTMEPRYDALYASLPRFAFATCYPGYVPANERTFTLGLQGFPANVSWNSTGIVWPLPPGVRGIKMDSGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVX7</accession>
    <name>A0ABQ5RVX7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003726</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14125">
        <property type="entry name" value="STKc_CK1_delta_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="10"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="309"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="360" mass="41500" checksum="14E12231BA859257" modified="2025-10-08" version="1">MNETIVGGKYRLGRKLGGGSFGEIFLGTNLQTGEEVGVKLEPVKARHPQLLYESKLYKILQGGVGIPNLRWYGIEGEYNVMVIDLLGPSLEDLFNFCGRKFSVKTVLMLADQMISRVEYLHSRSFIHRDIKPDNFLMGLGKRANQVNIIDFGLAKKYRDPKTHQHIPYCENKNLTGTARYASVNTHMGIEQSRRDDLESLGYVFMYFLRGSLPWQGLQAATKKQKYEKISEKKIKTPFEVLCKGFPQEFVLYFQYVRAQRFEEKPDYAYLRRLFRDLFSKEGWTWDYVFDWTILKYQRSANGAVERPVAVPEATGTRPTALQDSAEAGGSRVRREEEPDRNKEMARVGMRKTLQALFTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW24</accession>
    <name>A0ABQ5RW24_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34407">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34407:SF1">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047363066" description="SGNH hydrolase-type esterase domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="609" mass="64805" checksum="22066C82C2F373B7" modified="2025-10-08" version="1">MTSLISLPLFSILLGLSTADKHTAPITQNLTEAEVEYANIYWIYNATERIMPTAQGPSVIDSYPKLDYRFSLPSAERMAGITYVGHASRLRMVLERARHGETLRIGALGGSITSGHGVGGGEFTYLQYFMDWLNGAMPAHRRPPSSGGNDAVAAEAALRTGRALRAGGGVGGGWLERRRRRVLLRNKAAKHEFLNGAVPGTMSGYTSSCLTHHLIPGADLVFVEFAVNDSPLNTWADEGSPRRPLERLFRKLLNLPSRPAVVLVNMFAMGPSHGNYHHTAERDFMEFATYYSLPAVSLKAAVLPSAAVAGAAAVSLGAIFNGGLNHPGRGGHVVITELLITLCLDLLQLNTIQMGATLAARGAPGEGSSSATVAATDSGSSGGPLAMEKLAAVAARPLPHPIFPGNYGATQDTCYIEHQLQALTQPPVEGWEWTDEGRGKWGYVAMAPGKTLRLKVNTQVKPEGSDGALFEPIIVQISYLQSYRILGTAKLSCKSGCACEAMTLNAYDPRPVSVAITSDIKVTQHPECCLQLITGKAAKARAVNASTVRGFSSGWEGTTPTGSFAYKFKLIGLVVGEDPRATEGTVSFVRGNTADIFKKVQALRGASAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW48</accession>
    <name>A0ABQ5RW48_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protease Do-like PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004354</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041517">
        <property type="entry name" value="DEGP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046449">
        <property type="entry name" value="DEGP_PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980:SF18">
        <property type="entry name" value="PROTEASE DO-LIKE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17815">
        <property type="entry name" value="PDZ_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Protease Do-like PDZ" evidence="6">
        <location>
            <begin position="460"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="52"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="608"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="632"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="645" mass="70320" checksum="9DAA1E12354B5E8B" modified="2025-10-08" version="1" fragment="single">CIAVLSNYVFLHICFVLVLKFRLRSVSSMPRRKKHKNYVGALEEKAQALAFSPSSDSIEEKSRLGSPRTRSRSQKVDPTSTDGEAQVNAADPQRRNTRRKYGWGGTTSADAAAVDMATGAQPSGLPAKPRGSAPNSYTADSDRIMEAVVKVFCVHTEPNYSLPWQRKRQYSSSSSGFVLPGRRILTNAHCVDHYTQVKVKRRGSDVKHVASVLSVGTECDIALLTVEDDKFWDGVEPVVFGHLPQLQDAVTVVGYPIGGDTMSVTSGVVSRIEVTAYMHGSSELLGIQIDAAINSGNSGGPAFNDAGECVGIAFQSLKHEDAENIGYIIPTPVIEHFLTDYARHGLYTSFPCLGVEWQKMENPDLRAALKMQPDQKGVLIRRVEPTSAVSEVLRQNDVLMSFDGVDIANDGTVPFRSGERISFSYLVSNKYTGEEAELVVLHEGKQRTVCVSLRAPVRLVPFHIRGLPPSYFIVAGLVFTTVTVPYLRSEYGKEYDFDAPVKLLDKMMHGMASHPDEQVVVLSQVLASDVNTGYEDICNTQVVALNNAPIKNLADLAAKVDACAEPFLCLDLDYNQKVVLDTAKAKAATPDILAVHCISNDRSPELVKLTPPVASSKAGGQSAETPSDIKNKASKPRKRGRLVHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWR1</accession>
    <name>A0ABQ5RWR1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004072</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="228" mass="23897" checksum="66FCFC1F8FE28AF9" modified="2025-10-08" version="1">MALSMRTLSAKTAAPRGFAGRRVAAVSNGCRVVMKAGNWLPGSDAPAWLPDDLPGNYGFDPLSLGKDPASLKRFTESEVIHGRWAMLGVAGALAVELLGYGNWYDAPLWAVNGGKATWFGIEVPFDLNSLLAFEFVAMAAAEGQRNDAGGVIYPGGAFDPLGFAKDSSKAGELKLKEIKNGRLAMVAFLGFVAQHAATGKGPLAALGEHLANPWGANFATNGVSVPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX43</accession>
    <name>A0ABQ5RX43_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="5">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004584</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF5">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045874380" description="alpha-1,2-Mannosidase" evidence="7">
        <location>
            <begin position="22"/>
            <end position="1870"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="8">
        <location>
            <begin position="1598"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="288"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="699"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="833"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="896"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1118"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1312"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1491"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1729"/>
            <end position="1769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1838"/>
            <end position="1870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="699"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="746"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="782"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="896"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="918"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="937"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1122"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1345"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1369"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1379"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1508"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1532"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1733"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1758"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1838"/>
            <end position="1850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1870" mass="197448" checksum="D9899B903681DC36" modified="2025-10-08" version="1">MLCKLAVVLVFGIGFLQPCGSARPGKEVKAIWDDESTINSTHVDQLRKQAWEMFDHGFNNYMKHAFPMDNLLPISCSGKDWQGGLAITLVDSLDALVVLNRRQDLSNAVELVRGHLTFDKDIKVHVFETVIRVLGGLLSGHMLLDRNPDLVGAAGVVELSAKVTSAVEDLAAAAAKAPKGADMEQPGFGVQVRGPAKEKDFLQQPGAPRAEMASITAVESTAASQDMTMTVAEEGGSLPLQPNAPALHQPEMEQQPKQRLVEQAGQIFQGSSAHSERGELRVDQGPRETWTEQRGGHQEGLAGHQTEGASSGASAATVQEGVATASVGVPAAAAPAAEDGTLSAAAGAANSNPDAAAAAPGAKAYDGIFLRKAVELADLLLPAFETPSGLPSLFVHLKNGPIRDVHNSTCTACAGTLLLEFGMLTALTGNPVYLERAEFAARLLYDRRSRLGLVGASLNVINSEWASKESTIGPGSDSYYEYLLKAYLMFGKAEYLNMFADLYASTMRWMQIPGTFKGYSFIVDVHMDNGRLAKPFVSSLGAFWPGMQALAGQERDAIELHANFTAAWRTFGWLPEMFGIDLSKVHPEDPGYNLRPEHIESTYLLHCVTQDPYYLRVAANIQSTLATHNRVKCGYTNVNHVDTGEHGDLMESYFLSETAKYLYLMFSSAPGLIDYYVLTTEGHLIPPLPDPTALDVDKLKRRQEQQHQQGDRERSDQRQGGVGNEGSEEGKGQEIGNEAAGQKPPAASADADAMGTAAVEAAVDSKMQGPAAAEHHPPQPLHETQTQPPQQQDHNGQREVHEFSTYTDIAAAAMVEAAAKALRDAVFPAGRNTETAAGDSSGDTSQAEARAPVSTPGLATALLKTFRDPDLDPSVGSAAAVGPGSPVAAPTHVEALVEDRDIKPTTDIEDNGGIAESGQESRNPDSNSATVGTGKADSGGGSKVGSGVGDAVGAESSSSKVGAAAGPDELEVPQPIPTNCLKVCSSPSVQEQVATEARLRTAFPLLALRRSDAELIRHRRCVSCVVVTRRLEELPAENQDVRARIMRLPEQSIDELQTAGQADKDISPPNVLAQIVCAIKVSRSGSCTCSNLRAITGHDAQHGAPYNSVILQLSAMMEEPSSPLPPAPTPPWSSSPPQPGAAEALAAPTPRPKGTGGAPHDFDDPAPAQDVLDIFVLVAVRGGAGGEVSLLFEGMLAHFGPELPDTNPHCLQHLLAAPMMALTAGCDGHLFSSFFDAPYFIPNMMAPRTCPAYLQEALLRKASGAEAECRRFVFGAPHTDAARQVRAGSDAAGADGGAQIVNMAASTDAGHSIAGLSTLPGPPQWRKRMRRQPPRGCRLRQSAKAVSPAATADSTASSAAPLIDGQRAVPEKEQEIHHQQQQGEGQAEASKLLPVPSSIADVSPQGGLCSWDQSSNDNAVEGHCTMAAAAGDLLNEAAAVEGPYSSEIPNEDDGDTNVLCGASNAACEGKGEGEDCDDAGDVDWQAWPQDRKEGQDKEKKGHQHQHQRQQEEGQGRQREALRAPATEGIVGQGSSLADGSVAQQEVHQEASTVAAAAGDSAIGGPVHTKGVRNTSLSVDWSWVDKSHERGPCGGDPPVTGLLVPALPLDGCTRPRNPEDLVGAVAVLARGGCSFVTKAQHMQDAGAAAVIVINTQHPVELVGMGSDETGNQPDIPAVFVGGRDGRRILNAIGRNAGGLPLIASLSRLPPSMVSREGRAVAVATAFAGAANSGGRDNSGGSTCTAPPPAGEAAGRRRGSGGSGGSGGGAAKSITTRLELLVPTGSQAFLATQVMARGRTMQQLFEEVMEDGRAVSLLQQLAQKNARQVLAQMEQWRQMMRQQEQLRQQENPQRPEHHQHHHVPQQQQQHTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX90</accession>
    <name>A0ABQ5RX90_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004827</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007657">
        <property type="entry name" value="Glycosyltransferase_61"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961:SF38">
        <property type="entry name" value="PROTEIN O-LINKED-MANNOSE BETA-1,4-N-ACETYLGLUCOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045827632" evidence="2">
        <location>
            <begin position="28"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="851"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="857"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="876" mass="97224" checksum="56C7E5FAEA78EBEC" modified="2025-10-08" version="1">MTAWQSSHGGAFRLSFVLLLLIGRCGGLAVRARTPKRKSHTLDDILVANITSADVEHNDVQQFVRKGIAYMEALGIKLSEECERRYGYTFIADQKYHHFRLCKSEAKLSHNGKLTGVSRSQSALAKYRSRFEKQQQGRKDRATVSTTIKSKSTLKSAATAAATAGTGKRRSVLEHLWHHDEAVDRDTSAAASAALGRGDDVVNESINPRDGDPEGWTARQSLSHIDLFELEPELEPDGVDDGGINIRRTRRLQAAGNSSSLMYKILKYVETKKEADGGGDISDVYNDDDSDANADAKAAVMELEDADSSGAAATNATSTSATATAIDAERAAKKEAWLAKRRAAVSNAIEPDVEVPALTAINNTFKSSVDCYLQPSMPRNVGFKDAQLLCLGRNVVLDSCAYYDGQNEDFEVRFPKPKVGALKLGCSIRATLLGERNLTKGYKNRIWWMYAQDNAYDTVRQSCEPGSGHVVEHPVAFVLRDRYTHTLHELEVLSTIFTSLAVVDLPEVREQGIQIVLADQMPHASYRPTYAAISYPYRLRHLAEAPYPPNTCFRTALFINAFTNSIAFNPNQNTSECFSPVMIGVHKWLRNLHKELDPTYLARVHHHASETGGIVRRSIVWTSRRNLEALRLTSGVGMTEWQSARMVPNENEVVTALQSAILRWNSRSCLLARYYEGDQGLEGCRDSNVQFDLVFGEFSDWPYYPDQLMTIYRTGILIGVHGAALTFITSTTSGESALLELAGVGWEETQTGNMLNLFPSLAHNVGAYYEQVRYQGPDIDIKIATNALERAMDEVSRRITANQKHQQREMQQPRFNDQYNFDIMFPNACPTSVPNKLRRYVTNHPVQMNLNASTADNSSSGNSSNSSKSSFEAGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXK0</accession>
    <name>A0ABQ5RXK0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004976</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="247" mass="26962" checksum="05059AED39286F83" modified="2025-10-08" version="1">MKGISLNARMHRIWGAYERQLSRRPVLTQMATSCLLWGCGDILAQRVVEQRRIPDVDARRVACTAAFGAAFMGPVGHFWYQKLDVVCAKLLAAGSPSFLAAKLIADTVIMGPLYVVAFYAWGCALIDGSGIEGFKKKITQDFIPTYTAELAVWPLFQAFNFTRIPVEHQLLAVNGATLIDACFLSWARSQEDWVATAMGAIQSWHDGRKQLPAAEMAAAEMAAAAVAVVAAAPNRGSQHVPRQLRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYH4</accession>
    <name>A0ABQ5RYH4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09212">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036339">
        <property type="entry name" value="PUB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47694">
        <property type="entry name" value="PLANT UBX DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47694:SF1">
        <property type="entry name" value="PLANT UBX DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143503">
        <property type="entry name" value="PUG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUB" evidence="1">
        <location>
            <begin position="27"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="131" mass="14093" checksum="26FBE82CEA9E405A" modified="2025-10-08" version="1">MGYGGYVSAKLPPPKPSDVEAAVQAVKSMDAVEMIHKLVYNCAVQPKEDKFRKVRLGNSKVKAVLGDTPGAVEALTALGWSLEEADGEPVLVVPTGKFMTMQQVRVVEAARDKLAKTLKDSHRDNTSSLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYI1</accession>
    <name>A0ABQ5RYI1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_005348</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="502"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="658"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="203"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="355"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="535"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="630"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="925"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="358"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="635"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="942"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="685"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1168" mass="120572" checksum="C9C6D2B2C68BCD7B" modified="2025-10-08" version="1">MRSIECSRRNCLIAQLLVSALLCCYYLATVHGLQLRGLGLYGESSKSPSSAPSEISPGNSTVTDAVGFIAALLNSSVVTVCINRSIVLDSLGVRAQMQHILGQIIILNRNVTVVGLGRPYYLLDFGSVSGFINIPSRSTLEFRELRVRGLDDKLDVVPLFLAPSSSGNLNLHRVLLQRTACPLSFVWGSRDSTTITQYHGLSGTTGLAPVSPPTEQTDLDAGASSGPPPDGLEPAVYDTDRFCVQKNIADNIWEVDACYDQPILLLNVTKILEVPGQTHEIIHNAITSPASPPESSLLNKSRVTDDPFDYASPASVSVTVSFDNVFLLCDVFVNLTCQEMPSLLEVCIQELLSRKYRPPPGRTPFPARPRRAPPPAPRPATPPTLPVLPPPYPPTPPPPPPPPPVPPPPPPPPPPPVAPPAPPPPPPPVPPPPPPPPPVPPPPPVPPPPPAPPPPPVPPPPPVPPPPPPPAPPPPPPPLLPPAPLVAMSFFLDPATRPRAPLLAAVLPSVLGTLFVVAAVVVGIVWWRRCQRAAAAVPATPAAEKDGKLDPEESAYAAPGDEDSGESGRGTSGSKTSGKAFAAPFSRGTPIRYPLRRDTSSSISTTISTSAAGTLSGSAAASTALSSALTESTGPNSAAPTAVASPNGSAGNDPTRSIQLHHVLGAGSFGRVYYGTWNGQDVAVKIIPHLGNTNVKVQQEVALVARFNHPNVVRALHCATFNGAALDHQHVMQLESLRTSTITAGSPSPTLETWLVFEYCDAGSLATHLSARATLLAEELTAGAAAAANAVVTALCGIEPAGDPVGGLGRPEAGQQMAGVGAGAGPESIKPSVLAHLRAVLSVARDIATGMAYLHALNVCHGDLKCENVLLRTRQPQHPNGGASVPSGGGCAAAATAAPSSAAIAGPSAQLLPSERQPMVGATAASIGTTDGSSFGGGGSDGCSDAGGLRSPGSPASSASASASASTSFPPGANEPAAAVSAPLPKCSYAVPYVAKVADFGLSRAFNEASSHLSTGTLGTVTHMCPVLLQTGKLRPSCDVFSFGIILWQLVTGGIPFAGMRYAEIVYKVAVAGMRPEFPPHAPRRLVVMAEACWAADPNVRPTFEQLVTQLGELLEAADELQAEQDASIAAAREGAVRVDQVTFAAYSSEDVAVANQWAAAAAAALRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ32</accession>
    <name>A0ABQ5RZ32_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005169</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="4">
        <location>
            <begin position="49"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="141" mass="15986" checksum="878C588AB73AAE7B" modified="2025-10-08" version="1" fragment="single">SAYLIKMARTKQTARKSTGGKAPRKQLATKAARKTPATGGVKKPHRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSQAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S047</accession>
    <name>A0ABQ5S047_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thioredoxin-disulfide reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_006115</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006338">
        <property type="entry name" value="Thioredoxin/glutathione_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01438">
        <property type="entry name" value="TGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF8">
        <property type="entry name" value="THIOREDOXIN-DISULFIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0274">FAD</keyword>
    <keyword evidence="4 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0712">Selenocysteine</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="13">
        <location>
            <begin position="24"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="12">
        <location>
            <begin position="375"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI63226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="510" mass="55212" checksum="655925B0C9465BC6" modified="2025-10-08" version="1">MEGEPEAADNALAQSGISEEYEWDLVVIGGGSGGLMCAKEAAKLGKKVCLLDYVVPSPQGTSWGLGGTCVNVGCIPKKLFHQAGLLGESFSDARSYGWKLPEGVELDWEALVMGVQSHIGSLNWGYRVALREASVKYLNAKGSFVDPHTVEAVERNGTVHTLKAERIVIAVGGRPKYLDVPGDRELCLTSDDIFSRSAKPGKTLVVGASYIALECAGFLHALGFPVAVMARSIFLRGFDQEIAEMIGRYLERRGVRIIRPAVPTAFERDGEQVRCVFKNLDFGFEMSESFDTVLLAVGRDACTSDLGLDRAGVIVHPRSGKIPVVAEQTNVPWIYAIGDVLENRQELTPVAIKAGARLARRLYAGGTLQMDYDAVPTTVFTPLEYACVGDSEEAATAKYGEENIEVYVSHLKPLEWTMNHEEHNGQPIRGDNSVYCKLITNKVDKELVVGVHYVGPNAGEIIQGMAIAVKAGATKADFDDCIGIHPTVAEEFTILEVTKRSGKSSLKKGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S181</accession>
    <name>A0ABQ5S181_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="592" mass="65617" checksum="B89671B13A7428D3" modified="2025-10-08" version="1" fragment="single">MEAGVRQGCPLAPLLYLFVAQALFSWLKSQEIGLRLLPSDEDRVTACQFADDAEVALEGEESVPAFLEAMDTFARASGQRLNLDKMEILPIGAVDDHRQGSTINGLKVVTTATALNLPMDNQVQPSQPDWPEMLALAYTRMQRLAHYPLSAFGRAAAVSSYCLQTVTWHMEHGGPPPDTTLTYIERLAARLVDRQQGPEDQGQRFTGVPSRLLVGHPSTGGFGLLPLRQHIRARQAMWAVRFIRGALMARSEGERVTLLDTAMNTVAEEEEEPATSGNVRSLHPWIRTLATYLEITHLAFKPWALTTAQATGPWLGGETLPEEISRIVGALGHLPPLEDVSKPPLAPGVWCWAAALWGNPLLPNNSDNGGSVNGNNHRPGLETRHPLMASCRKLLTVGDLVRVYDMLEHDQISLPQYMTWLDWTQQFLDPSASPTQLRDKESTRMALSALCNDISPAWIAAAREVVNRLQPQQPDRVEDRMQQQMPAPPDRTTVMAMIVRRLGWTLPGDSGIVPLYNLTVSQATQLQGVSTAEERLELLSAFVQEAQGMPTDGPTPPCPVTDLQRFQATLDRLWTAVRWENKNKETLWRLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2S1</accession>
    <name>A0ABQ5S2S1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPI transamidase component PIG-T</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007245">
        <property type="entry name" value="PIG-T"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959:SF11">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04113">
        <property type="entry name" value="Gpi16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046614075" description="GPI transamidase component PIG-T" evidence="2">
        <location>
            <begin position="24"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="640"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="682"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="725" mass="77848" checksum="EF87155DC44DACEB" modified="2025-10-08" version="1" fragment="single">SLMLQYIVWTAGLMLAIAVGCNAQDVFSEELLTAWVSPQHLLVHFRFDQGTTASRLHTSFPHAIQQLASTLRLHAVELSLTSGRWRYTTWGRPLVAVTPVGAVLDASLRAGLKPQALIAHWAALVNALSGLSCASFSLLHDPTSISGGGISLRQLLAPTTGNRSAEGPVAGGQRLRAMLPHEPLCTENLTPWLRLLPCRDQAGLASLLRHRPTVFGAEYVSLGLMLERHATPKGRRGVRLVQTVTLVIRTALKRAQQQQQAHPSAPGADLETVLGARVEHFCPPAMRSEVLVAEPDSPEGLFATWRTRSGGVCCESEDESQNGSCPLGDYAAAATTGTLDAHNTPGPVREPSRLLLPVMIPAGSPGFAVEAVSQALPALRRYDTLELLEVGDRALGLQLPYPHPQPSTQPHIAGVHEMRANGDDRLAGPDLQADRYVTGANLLHGAVVLRIQRSRELRARLEASQCTGWRGSGGAEGGGTCGSSLVCINQLFPWYVRPWMHTLTVLYDGQEVPLRPHLVAQQVRPAVQRSSPGVVDLCLRVPPAVAEVQLRVAFSKAFLTVFEYPPDAHRGFDVPAAVVSYLDPLYAPAVQWRVTSPGDVSGAEVVSSPLLSGLSAGPVKQVYTVGLLVPLAAPDFSMPYNVICLSSTVLAVYFGALLNLLTRRGCAAGHAAPERSKVSLGGKALQALLMLVLFSALALYLDAELRQHAMELLAKLGFPLEFKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S306</accession>
    <name>A0ABQ5S306_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_007197</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family. CPR flippase (TC 3.A.1.211) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="503"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="545"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="580"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="608"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1820"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1862"/>
            <end position="1890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1902"/>
            <end position="1921"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1933"/>
            <end position="1951"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1971"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2038"/>
            <end position="2055"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="756"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="2176"/>
            <end position="2439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="314"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1744"/>
            <end position="1785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1750"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1765"/>
            <end position="1780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI64061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2456" mass="260195" checksum="FC94E71087A975F8" modified="2025-10-08" version="1">MYRSLYFALLLLQAKFRGIKFLAAEILSPSLIMVVTFVLGWAISTTDRYKVLIFADGSHHFIHAQADILRRAGWNAGIPTCLSPLARATESELVQRQRDREAARRHELWQQEHFSRNRSSIRQGNDGLVTATDDSQQRPQWQLLKGFGGTEWIFASGNRGSLGELVGREVVMALLSATKKPGVSEALEGCNWQEMWASALPLTINNIFNQMLSPTALLTFDEWLLMGSFINATLEASGKKNTLEATLELLMPVMPVSGMQPGWLALVPDTPQVRAFAAHMSATTRFFDDVFLGTYDSVAAAMDAVAVAKTSPAPRAADGASAGEINSNASGEAGGKGRPRRRPLVTPGRLWAVIEFVSGPEPGRGVDYAIRMNAMHVPRTFSKLSPLDCKTCSNDFLPYVASGFLSLQAAVSGYVVDLGGRGQGSLNASAGARAVGRSPLSEETAWLHKPAFEATGDGDGSSRQRDGSEAQLGVSSNPYGIWFTPLPNVGQEINAFYEYGSPLLGFLTTCSFALPLAMVVRHMTSQRQSGLQHLLEVVGLSRCEWLLAWLLAGLLPLAASFAAEGMLAQSTYLELVQPGLLAAVVGAAALAAAGWAVLLGCCFRDAKVAATGCFAFTLAVGLPFLALYTTAPMQLIAVKRTCCLMWPFALSVILQRMAAGHQLRRYGDSRSVSDVPGLNWSTMTHGPLSSAELLGFLLLDAVLYLAAAAAVLTWRGLSVGQGAGSTWGFGKQRRGCSGIESGHEARGSGDDRTAVLKLNGVTHRYPGNEHRALDDVSLSIGAGEAVALLGRNAAGKSSLMAVSCGRMKPNGGGVVRVAGCIMYGRNGVDDVPYRHLGYCPQGNVLMPDLTAFEHLQLALVLRGATEVGWVLPGRAGGRELRAAALKLATLVSLPQDMLQLLVSQLSGGSLRKLQLAIAMAGEPPLLLLDEVTAGVDVESSQAMWEALVAAKQGPWGSSAREGGNGSGTDCPAVADMERAPHEHLHRPTGRGAALLFTSHHLSEVSKLADWVAVMQQGQLLSLTPAAALHSLLQEGDQRVLRITTRPARQQQQQQVIAVALASSSIPPAIPYRSFTRSQLLELILRRLGPSTTRVLYSHLTFSAVYVEIAATAMGAVGASKVKFNNGSISTAPSLESLEQLLQELRPQVGGAAGTGPITTTPIDTFDRVQVNCGLPGGGSASSSRGSQGMDSEWGTTPLMVVRCGLGRPTLEDVLLLGLGPRGASAQDKGQSPHMSLQNTARLGIGEPHDGAIRGKTTDSPAASIIKFPVASKKSVGNIADTTTTANLQSINVGSFHAASPQELRPGGFPLPGSNAAEAVDVVVDACYCADGRSDVLTELMASLPPLLTKQRLTWLRDHVSSARMLLLPVVVVGLAILALSIHPLSTEISAPLNLAWLGQDQPTPVAGLPPAWRHTAHTTTPVATFNGDGNIVGNVSVPRAMTAAVNTSEAGLVGLGLLPLQAEWMSAAGAGKDPDKFPSSSDVSVWFAKRAQATSAATTYGPLGALEKEPSAPLQSALVFGDVVTARLNHTVWSAAGILMESLLAPANYEQFGLTDVPELRKAIQVLSTEEGALWKMLDFLYDRMLLLEVVSLVQYVMHHSGQRQVGTATATATSDSTRGTGGLDRSNVLNVDRMADLCQLELPGSEEEGKGKSAGGRTLLMRYRPPLEVSLYLLGSLLPPERRFCPLARLFVEQSPAVVKVIRRYVARRKLPALNLMWNISSPHGLPATLNHLHTTLLRLGQQTQHTHNTSKHTHRAHQQRHGSSGGNGVHAGSRGGGGGEERGTYADRVFKSTAIRVRSHPLPGRGATLLDRGLMGHLLLALMVAMPLSYTTGNFVVRAASEKASGAKHQQLAARRTSSLSYWLSFWLTDLALHALITAAIVGLMLLGGTEAFVGTSARCAGLAALLMAFAAAALPWGYCIGLRCANPASAQGLVSTAGLVVGVGLALARQILSHVPRWRWLADMLLPLLRLLPPFCLVDGLIQMSLFDAILVVSGLLGKHASRRGLLWSLFEGHLVGIDPCDHSPFQNAVLGSNLFYLVIDALLYGVVLLALERRMSHGSLFSGSPFPGWRLPRRALSAFKGIVAAIRALPAKVSSQYPGGIDDACGAGFDNACNTDATASIHLRQVKIVGKGASGGSGGGGGGWVSEPGTPLKQASCESTGGVSEEAGEFVVRVRDVYKEYGRGRDRLVALCGLSFQLRRGDCFGLIGRNGAGKSTAMAVLSGRLRPSGGSVVVGGLQVAGRLEAARGRVALCPQTNPLLPYLTPTEHLDMYSKLYGITSSVRGRRAGGAAATLPIEAAIMAARCGLPAELLHRPAGQLSGGSQRKLSLAISLLGRPQLLLLDEPSAGLDPPARRNMCDAINMAVRDWVGTSSEGSGHGAAPSSAPAVVLTTHYTEEAVALCDILGVLEAGRLVALGPPEQVSLQLCALRLGHYLEDFECEWDTVLTPLQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S344</accession>
    <name>A0ABQ5S344_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007542</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="10180" checksum="51E6B106C7CDF195" modified="2025-10-08" version="1">MLAPASVRGATVPAPTVGLQPPTKQYVVVQDDVCANASIGGPAPAGCVPPEGASLRVGLRIHYEASHNLHGNSASAADNYEFWSCPPSWLKFSCCGDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S359</accession>
    <name>A0ABQ5S359_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_006969</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16655">
        <property type="entry name" value="RING-Ubox_WDSUB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF33">
        <property type="entry name" value="PROTEIN HOS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="182"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="5">
        <location>
            <begin position="608"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="283"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="489"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="678"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI63859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="697" mass="74764" checksum="AAE35BC3E0D31AC8" modified="2025-10-08" version="1">MGNFVSTPDAAFKPSDAFFIFDAAKNGSIDIVRLFLEKSKWLAYATDTEESTPWHVAAAEGYDAVVKVLIDAALVNKTSEGSLLKLINRKNAKGQTPLMLACGRGHVTCARLLLDSGAALLFTDATGRSALHHAALYPGVDSKGDCVGLFLEYMQRRLFLERHKFETHASIIRRHADAADKYGRTPLHCAAWSGNVRAVHTLWMVGADICSQTTADCFDSDLPCNRGSTALHFAAIRGHQQVVVLLLAAWHRLVTRPESPLPRPAYAKELMRERQEAAEGRIRYTVGGEREPEPAKAPVQDQDEEGHGALQGTSFQSGGERCSELSLPCGESDGLEEHGTESGKGQGTDQGPEAAGTCGEIETEGQTGGPSTKHQRDTALPENGASAPGAVDVRASEAASARGFEPASGTGKDGSDGISNSHVKDVDVPMIKRVDFPPPPLPPPARIDPRLIVDGYGLSPYDYARKNKEQRGLMFVELLDAATDFCVDDMEDESGSEDGLPECGVGEASSGRGRGRRGSRAYGRGRGSEDDDADSDVAAAGSLLLWDGVELVQDIAADPDSFFTWQHSARFLQSHLEGDGKEGQTSAKEEHGRPIPTIPSRFSVTTEVIPDCFLCPMTCKVFRDPVVAADGVTYERDAIERHLRHVSTSPMTKQYLPSKQLYANNALKSAIAQWSSGLLPPGGARGGDDRIGGGTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3G7</accession>
    <name>A0ABQ5S3G7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044664">
        <property type="entry name" value="STR11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45187">
        <property type="entry name" value="RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 11, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45187:SF2">
        <property type="entry name" value="RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 11, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="90"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="276" mass="30068" checksum="C82E320881A91330" modified="2025-10-08" version="1">MDTTVKSTNKQSQTVPYSRLISAAPRAVLLRPCGRRVALFSPALPQRTLRVQAQIQQQQLTEGKDEQRWDAQVTEGKVRSLSGSDIRQYLAEGWLLLDVRPPSEARKAHIQGAVEVPLFLEDDSMSLSSIVKRLSAFGMGGWWLGGTHMVPNPKFLEEVQAKVPKTTRLLVGCQKGLRSLAACEQLSIAGYDTLAWVNGGFDSCKEGEVPTVGDVDIRYAGIGGLSEAIGWTEVQQEQNKGLGSVDGALKLVGLILVADLVLFAAEYVQAYMKGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3J6</accession>
    <name>A0ABQ5S3J6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009543">
        <property type="entry name" value="VPS13_VAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF93">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25036">
        <property type="entry name" value="VPS13_VAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="5634"/>
            <end position="5773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="910"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="991"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1130"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1182"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1470"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1575"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1634"/>
            <end position="1653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2016"/>
            <end position="2037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3349"/>
            <end position="3380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3604"/>
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            <begin position="3771"/>
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            <begin position="1500"/>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="GLI64033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="6537" mass="704879" checksum="D2D58D1F4210E2A5" modified="2025-10-08" version="1">[encoded data, 6537 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3P7</accession>
    <name>A0ABQ5S3P7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_007422</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Na(+)(in) + H(+)(out) = Na(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF197">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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            <end position="63"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="630"/>
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            <begin position="83"/>
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        </location>
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</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5F1</accession>
    <name>A0ABQ5S5F1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007991</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="234" mass="25215" checksum="81FFBDB46B1C9357" modified="2025-10-08" version="1">MLAMKSLPLRAGRLSTVRVQAVQVKTRPKSAEQRFKQATGLPAPDVNGKQFPVKLGFTKTNELFVGRLAMLGFASSLIGEVLTGKGALAQFGYETGLNGIEVDGLIVGLIAFNLIAAILPTSQTFVPEEQESIQERPAGPLQDPRISLLDPKRFFGVKGFGFTKDNELFVGRMAQLGFAFSLIGEAITGKGALAQFDIETGLSLRDTEFGLVVFILFLLFAAVNEGTGKFVDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5N1</accession>
    <name>A0ABQ5S5N1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">VaNZ11_007954</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="20">
        <location>
            <begin position="77"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="19">
        <location>
            <begin position="230"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="517"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="618"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="23">
        <location>
            <begin position="698"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="17">
        <location>
            <begin position="746"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="1129"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="GLI64642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1223" mass="134461" checksum="4B2B152FF90941D7" modified="2025-10-08" version="1">MTVAAEAAGGKGAASMDVDSSPASAKGCRHGPDGLAAVGGDEALADALYGDPTRLTEPIKAVQDKFALLPAFLKVRGLVKQHLDSFNFLLNSELRKIVGANEKVNCDTDPNFYLRYTNIYVGGPSVDEDMVQVGTTPQQCRLRDATYSAPITVDIEYTRGKEIVVKRGRGGVGAVCIGRMPLMLRCDRCVLYNKNEAELATLGECPLDPGGYFIVRGTEKVILIQEQLSKNRIIIDTDSHGEVIASVTSSTHERKSKTNIVFSKGKIYLKHNAFQDDVNIMVVLRAMGCESDQEAVQAVVGGGFGARTVGGHNGGGGGIVPDAASGEGAAALAALLVPSVQEAKGLGVFTQMQALDYLGGKLKVSPKFGPGAVAAEKRRKSKVDEARDVLANVVLCHVPVSRYQFAHKIAYVAVMIRRMLYAVLDPACIDDRDYYGNKRLELAGGLLSLLFEDLFKRLNSDIKRQADAVLSKANRATQFDVAKAIRTDTITYGLESALSSGNWIIKRFRMERKGVTQVLSRLSFIAAMGMMTRMTSQFEKTRKVSGPRALHPSQWGMLCPADTPEGESCGLVKNLALMTHVTTDEEEGPICRLLLMLGTEPITSIAPAEMHGHGGAMVFLNGNLIGVHRRPHRLVQEVRELRRAGHLGEFVHVHLQQDACYVSADGGRVCRPLIICDAGVPRVRQEHIAKLRSGELTFPDFLRRGLVEYLDVNEENVSLIALYEKDCSKLTTHLEIEPFTIMGVVSGLIPFPHHNQSPRNTYQCAMGKQAMGNIAYNQLSRMDTLLYLLVYPQRPLLTTRTIELVGFDKLGAGQNASVAVMSYSGYDIEDAIVMNKYSLDRGFGRCIVLKKYGCSLRKYANRANDRIVAPLPVPGQKTVAPRYRLLDRDGIACVGDYIMPGDVYVNMQRPSNTRDPLPASTMPDSFYKPSPMSWKSTPAMAGERCIVDKVQITANDESHHSVKVLIRHTRRPELGDKFSSRHGQKGVVGNIVRQEDFPFSERGVCPDLIMNPHGFPSRMTVGKMIELLGSKAAVFSGRFHYGTAFGEPSNLADKVEDISATLVRHGFNYCGKDFLTSGITGEPLEAYIFMGPVYYQKLKHMVLDKMHARARGPRVVLTRQPTEGRSRDGGLRLGEMERDCLIAYGASMLLLERLMISSDQFEVHVDARSGLLGWWDANRNCPVSPLDKSSTHMATIKIPYACKLLFQELQSMNIIPRLRLTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S662</accession>
    <name>A0ABQ5S662_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44843">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44843:SF14">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase FkbM" evidence="1">
        <location>
            <begin position="510"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="697" mass="78748" checksum="DD52E3C0034604BD" modified="2025-10-08" version="1">MLARTSRNAAAALLVVCNVILSFRTIYAGARLLETATLTDPAAPSPELLGELRNRVISLVSNITYDRYKYVGVGEVRRISNSQKGFHMNGYFDKSPWSSDGSKLLFQRVPFYDRGMSELDVLEIGIIDFDSTSDTQLEILTNTTAWNHQQGTMLQWLGWSNSVILFNKRNTPNTFVGTMYDIVQRREVATLPLPFYSLSFDGTIAVSLNFARLYVARRDYGYAVDHAVQTLIRANTCPDDDGVWVMTGLQDPPNVKPRLVVSIRQAFEIAARPDAVDPITQLKYATSFNGLDSRAIQTECVHWINHAQLNREGTKVLFLYRVGRCKYWRGFKTFVFTADVATGELWRVPLTHGSHHDFGWDDNLISCDGSGYYHAKFKTFAVSLRRPEDVARGGDGHCSFAPSSNQFILSDTYPRPIPGFQAPARALFTWDLVRNRTTIIGYYSPVKESTDIAQRVDLHPRWSRDGRYVCFDSTHEGLGRQVYVAKVYSKALVRPGVDVVTRPPRKFFLDLGARTGDSVEKMLIKTHTDYSDYIIHAFECNPENMPLVLSGLDRLKAEYKLLPENVVLVEAAAWIQKGTLSFHMDSRKVGNDGQPGKTGGSLFDSPHAQGSLVTVPSVDFSEYLESTVQYVDHVICKIDIEGAEYELLTHIIRRGSIILCDVIMIEWHSRFRTEWKGWDAEFEAMMEQLHIPVIKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6E7</accession>
    <name>A0ABQ5S6E7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nucleotide-diphospho-sugar transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_008213</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF11">
        <property type="entry name" value="NUCLEOTIDE-DIPHOSPHO-SUGAR TRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="440"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="52699" checksum="938535B8010AB683" modified="2025-10-08" version="1">MASFAASRLTHQPSPTTQACIIVALLPIILHIFLWAMRVPHLGGEALLQVALPPNCSMHPLESLLIQQKDTFENICFTPAAEEGAAADAPTPFATVVTIQAGLSAQFKSMIRTNRLIYAVKQGYRYCEINELPDPTRRASWNKLAVALGLVLSGCSEYLFIVDADALIVGTAVRLQDLIAAHDPARRASMLISREYPRVGEPSHINMGVFLVAQRPGAADILSHVYNWHVNAHQHQEQRGFNDHLNAWMEQERRYGAAAADGAGTVGMDFSQPSNNVSAAVTPDRDAAARPTSATTSATPAVDAASAASGLLLVDHSVFNQHLEIYNRMPMEQRRRTFVVHWAGPHGGTDKLTVMRADIAEMQDLRRAVAVEAADAASPLCIGAPGIRIVQRSLGWRFVTWTSKRWAKSYLAFYRLGLWSKLEVLSVSSAVVARGQEPRRSAAQWRAAGKPEGARAGSPAALHRRSATQHSEPVLAWMRMQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6J8</accession>
    <name>A0ABQ5S6J8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_008750</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="10">
        <location>
            <begin position="144"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="190"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="232"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="404"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="435" mass="47224" checksum="2DBBC1370B0A1E13" modified="2025-10-08" version="1" fragment="single">MSGVQPPTPGKAGGSPGGGNKSVKLSFGTKTPGNGKGKEKLDRFIPTRSAMDFDSANFNLSKDARQSGEQRGGQGSPSKEEYHKALAASLSVNDSNRILAFKHKAPAAPEGYENNLKSLYSQNVAPNVTKKQFRHVPTTQERILDAPELMDDYYLNLLDWGSQNLIAIALGRSVYLWNAGSGNVEELCTVPNDGDYISSLRWGSDGNFLAVGTSDAKVQIWDASRRKQVRELCGHTNRVSCLSWNASILSSGSRDTTVANWDVRKRRDEACVASLRVHEQEVCGLAWSLCGQQLASGGNDNILAIHDASFGLVAKVQAHTAAVKALAWCPYQSNLLATGGGTADRHVRFWNTHTCAMLSQIDTGSQVCALQWNPHERELLSSHGYSKHQLCLWKYPGLVKVAELSGHQGRVLHMATSPDGCSVVTAGADETLRFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S706</accession>
    <name>A0ABQ5S706_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF154">
        <property type="entry name" value="GRIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="103"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="603" mass="64938" checksum="8F87F56C38966CC6" modified="2025-10-08" version="1">MPLLGKHHNPFFILGRKSARHRSAQPKHARAGRAPVACLAATQQALSLPTVLEESRGECLLQARIGLGERGRGLFFAPFDAPPSDDCGTQDIMATQTPNQQQEPAVLLRVPLSCGLTLVTGGGPQDLQRELLCEWQSYQGLRLPSQLAALLEATERPPALRLAAWLLWASQVAPAEVEAGGSEKEGEGGVNGGGQAPNECPGDDATSSGDKASCPQPSPQQPSLRPPSQSLAPVPSSRPHLLSEYARHIMPPPNAAASLLRLADDQERRLPGDARAALRGVRMQVKSYISELRHLGLDWGLSEATLWWAFDMVSSRSFAARAALPTPQGPTDPISASYNPSPRSNLTGNPSFADAASRPPSALVKLALYVPWACLLNHDSSPNASFEADLGKRRFQVMQRSSGFSFLTTKSLVPGEELCISYGEDLDNVQLAVRYGFCTPGNTNDRLPMPPELVAVARGLRRQMLCSAAEQVADDLSRNVVDGAEGSLQQRRSRVFSAMASLLDPVVISQGSRSMDGFLQGPVAFPLGALLSWSENAHSCTKEREVKIHGDDLQSGLVSILPLSVQMPLGSLCLDALREERKFMATSLHKILQIYCNAAASWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7P3</accession>
    <name>A0ABQ5S7P3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_009624</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130">
        <property type="entry name" value="CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130:SF171">
        <property type="entry name" value="OS01G0895300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045791117" description="Cytochrome b561 domain-containing protein" evidence="10">
        <location>
            <begin position="36"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="542"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="581"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="608"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="11">
        <location>
            <begin position="275"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="12">
        <location>
            <begin position="426"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI65943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="695" mass="73937" checksum="8277B415CF1C6FDC" modified="2025-10-08" version="1">MNVRLRCLRRRASQDEASLAFLLLTLLATVQLAVSTPTYFLDPARALINDSQKCVAHPSSTTSAKGIQKHGTPVLDSNITFEIYNIEGNAQAAVLCPGSDYNLKVAFGGRDRAALLNSNPPLVTFTLPVPTSGCPNQVVQPSSNAAATFNTRFTVSCNAIGQTLVFKVTSAGRAEGLTWKYASISAPVASADSPAAAPGGTCAAAIAACGLSRPLPPRPPPPSTASEVQLSETTGASTCTPSTLGYQCMVRKDKVTVHWTLNTKAAPNNPCTPTSKTMLKDTEVVQYGTLHMAAQSNTKGYVGLGFAENPDDMYRSDILLGWLDGAGKAAINTFFATRNGIDMGDVVTDTWAYDTGVFRDSAGVTTLCFSRRLWDNRAKISQDLRAVAGSSSKSSSALGSRRRLLDATAQGVLTQQLGLIWALSPSPRLVQHADEDAGGFYLDLVSGDFAESVDDGKSESYWVNVHGALMAVAWGLLLPLGTLLPAHRWLLRDVKVAGKHLWFWLHLGCQWTGVALFIAGFVVAFVKFEEVEGDMSEAHEKIGIAVMAAAAAQVVVAYIRPDPGAKRRGLWNLVHHNLGRITILLAWANVYIGIYLAHTGWGASYTEWVTPIAIVMGLLVLLDIALRITGCGKEDVAAPARNDAYKESYDDPAKIGDGAEGKGPSSFPVNSLVSSDFAVGASVKDMPLGHPKSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S803</accession>
    <name>A0ABQ5S803_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="18667" checksum="3ABFB8679B3DC0B9" modified="2025-10-08" version="1" fragment="single">QSILWDPSDLAGWLETEAALKVRGFVPLEGSTVTRRTIVNHTGEKPYSVHIQLSPEAYHTCRRQHVTVVASSEDLQGLWYRALTQACMPRRALMARLQHSDMPKECTSGCPSLECTRSRQARRLPYLPPGPDGVSYYSTASFRAVHPTKGPAAAAIDAAAVTGGSPPQGPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8R1</accession>
    <name>A0ABQ5S8R1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="122" mass="13760" checksum="534D08C33B9D0573" modified="2025-10-08" version="1">MGKCATLRALLAVVAEQNLELHQLDTKTAFLNGVLEEEVFTEQPPGYQLGGENVACHLRQSLYGLRQAPRAWYVCLCSVLEQMGFKPLVDNQGLFVKDENGEKRCTWMIYLLPTSQSAWLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S904</accession>
    <name>A0ABQ5S904_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45274">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45274:SF2">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="2">
        <location>
            <begin position="90"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="379" mass="39717" checksum="F6DA12E3A526BD13" modified="2025-10-08" version="1">MLGKPLLPVCRAKVRPSPLCRRPLSKIHLPARALTTVMSVVPPALGSAAVIAVGAALSYVCYKLIRLAFADADLHLLGLGKHKSNAFKDKVVWITGASQGLGEVLAKYFAGFGARIILSSRDASKLERVKVSLGVLDERVLVLPFDLCSDYTELEKAAAAADSAFGSAGVDYLIHNAGASQHALASETNAEVTDALMQLNAIGPIKLTRAVLPHLLRRNRGRVVVVGSMSSKLPSSGQAVYAAAKMALYGYFSSLATELADTGVGVTICCPGPLATGSEETPRVVFGPTGRIVQNATGASNRLNAARAAQLIACAAAHGVDEAWIALHPVLAMGYIFQLLPRLGWSLLKKVGPARARAVKEGKSGYDVSKLMKAGGKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9J0</accession>
    <name>A0ABQ5S9J0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009969</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="470"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="697"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="704"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="742"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1352" mass="149270" checksum="BEAB081C55ECDD0C" modified="2025-10-08" version="1">MGKDRLDEPLDLHNYSTWKLRFQLLCAELGYSRALLQAPIAEADVIISDKVKSVMAKNVKNHHLQTIVRAANAKAAWDALAATFASTCNTRKVALRQELSDFKMANGEELPIYISRARQLQSDLLDVGHAVADTELSTIVIKGLPSKYHVVTTALTMREGELDFTQVVARLMAYVIPDEDNNESSSAFITHRSGGKFNPTSRHQTSKQRSKSNAICHYCGKPGHFIGVCKKRLADQQQQNNGRGGGNLQRREPTKMAYAVGSESRSDAWHLDSASEWHITYDASELKDLRAVRPEEKFTLVGYDGSRHQPTHVGQYTMWSNTVKGLQIELKNVYVVPKATVKLISAGIFDERGATILLGNDKALVQAKGRTILRADKVGRLYAIRHHAAGRSVGKGRSCAAVGSEGSKAALWHRRLGHLGYKSLERMCREHMVTGMDLDRASLEAARSRICEPCIYAKQTRGPFPSTGHKAAKPLGLIHLDVCGPMPETSIGGSRYVTTLLDDCTGFSTVAFTDTKDAVRKKVKIMIEALENMTGRRVKEARTDRGTEFLNTVLGAYFGEKGIIHGTTVGYSPEQNGAAERLNRTLLEKTRAMLAESGLPQKLWAEAMATANLLRNVSPVVGASVTPHEAFMGVKPDISHLRVFGSTAYAHVPKEKRNKLQPVSKKGVLVGYEQGRQYRILINNLISTHSTVKFDESTVGDRYEDSDEEDVEESGGVPLPGGGGDTPVNTDMDQGAAEVNHGASTSSGQTAAPQRHAGIPAAQFPRMQNTAQSRGGNGGDAMTGEGPVQQQNHNNDGADVNMQERRYPLRERKRLIEWRHEPNGRVTFGRINAAIEEEVPEPASYQEATDGPYAEEWRSAMDEEISAQLTNGTWELAKPPPGARILPCRWVYKVKRAEDGEIERYKARLVAKGYEQRAGIDYGELFAPTSRFASLRMLLAVAAAKAMPIHQLDVSTAFLNGELEEELWMQQPPGYESADPEQACRLLRSIYGLKQAPRCWYMKLAAELDKLGFKPSVADPALFIKKDEKEIVYLLVHVDDIIITSEDEAMIRRVKTEIGQAFKIRDLGEAKVFLGMEISRGAHGEVKLSQRRYIEELLQRHQLVNAKPRKTPLPPGTRALPAEDNNKELVDNTKYRALVGELNYLATSTRPDISQAMSVLARFMGNPSKVHMGLALGVLRYLAGTKELGLCYDGDGDLTMVGYSDSDWAGDPATRRSTTGYVFLLNGAAISWNSQLQRTAAASTVEAEYQATAAAVREALWLRKLTRDLGLRTQAIQIRTDSEGSMSLGNNPIMSVRSKHIDVQHHLVRERVLRKEVALTHCSTEDMVADVLTKALGEIKFKKCITAMGLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA16</accession>
    <name>A0ABQ5SA16_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Elongation factor 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Eukaryotic elongation factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">VaNZ11_009743</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18626">
        <property type="entry name" value="CD_eEF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015688">
        <property type="entry name" value="eEF3_ABC2_chromodomain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047036">
        <property type="entry name" value="EF3_4HB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF5">
        <property type="entry name" value="ELONGATION FACTOR 3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <feature type="repeat" description="HEAT" evidence="15">
        <location>
            <begin position="185"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="534"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="781"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="16">
        <location>
            <begin position="910"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GLI66036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1088" mass="116846" checksum="2A13C723694D0D41" modified="2025-10-08" version="1">MLRSSAPAFNPSAPMFAPVGAAPRPLAGGSGFLSPEVAAIEDLLGRLSLAVKEESTVAAAKPAPKPAASMASAGPKPAAPATGGIKIIKAGGPAAAAAPAPSGPWASGATSKGGAGIGRDKAAQEVGEYVKSRGVSVLKTSGLLRKLAVMADDPNNAVARETAMWACASLCDEAGSAVEPFMAPMLPVFLERLADKATSVRGAADAACQALARLLHPEAVPRSLPAILAGTSPSRKWQSKEGAFKMISTLCETAPEAVQAALPDIVPVVSDGMVDARDEVKIVAVAAAEASFALAGNRDIAPCIPSFLSCIRKPKETAEAVLKLSGTTFVQAVEAPALAVMCPLLIKGLKEETAIKRKAANIISNMSKLVTSAADGDTFLPKLLPRLEAAANETPDPECRSVCAAAVATVRRVAETAAATRQAEADERKVLGEKEVEAMLKEVLQANGVNPADDDLLETAVSYCASIATCLVNKRSTESSKWLEKIVEPFLLPFLSEGDSAAAAVADAWSSQCEAASHVVLDLGLSDDEAGEVLCNCEFSLAYGGGGKILLNNARLRLKRGRRYGLCGHNGVGKTTLMRAINNEKVEGFPPKEDVCSVYLESDIDPTLAEVPIVDFVFKDPTLQVKAQPPSMEEVVEMLTNVGFDDTKQKHQVGSLSGGWKMKLALARAMLMKPDILLLDEPTNHLDVVNVAWLEHYITKVIPHVTCLIVSHDSSFLDNVCTDIIHYESRKLKYYHGNLSEFVKVKPEAKSYYTLAAATLRFNFPKPSFLDGVTSGEKPILSMKKVTFTYPGAPKPQLESITCSCRMSSRVAILGPNGAGKSTLIKLMTGELKPDSGTVSKHVNVRVAYVSQHALHHLEDHLDMTPNQYIRKRYSKGEDKEDEHKVTRILTPEEEEAIKKQIWVIDGQNRVLEKLLCRRKKKKSFEYEVKWVGLSSIEFNRWIMREVLVERGFEKLCREMDDKINAEKGEAAARPLTLASVEQYLQDFGLDPEFGTHSAIRGLSGGQKVKLVLAAALWHCPHLLVLDEPTNYLDRDSLGALAQAIKDFGGGVVMISHHSEFVGALSNEVWDLKDGKLSCHAKTAKDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA97</accession>
    <name>A0ABQ5SA97_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_010180</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="564"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="594"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="654"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="799"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="707"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="94"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="720"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI66388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="866" mass="97853" checksum="3FF4005A0019E809" modified="2025-10-08" version="1">MNKLLDFGFQNIDLWRSEEMELVRLLIPSESAHDTVAALGEVGLLQFQDLNAEKSAFQRTYANQVKRCDEMARRLRFFQEQVEKAGLTPAVRGAPTDKHELDDLESKLEELEKELINMNENTERLDRTYNELVELQVVLEHAGKFFDKAKATVRVEAFDRDYSGVQENPDAPLLEMGVQEKVARIGFVAGTIPAERINGFERLLFRATRGNMFLRQGSVGAVKDPITNETASKHVFVIFFAGDRSKVKIMKICEAFGANRYPFPDDQARQRQMDSEVTARIRELQTTIDAGERHRKSLLQTIAANLDEWATLVRREKAVYHTLNKMNVDVTSKVLVAEAWVPSATKPEVQRALKESAENSSTQLHVIMQPVVTHEMPPTYFRTNKFTTAFQNIVDSYGVARYREVNPAVLTLMTFPFLFAVMFGDFGHAILMIAFAAFLVYKEKQFLKQDLGDMLSLLFGGRYVILLMGIFAFYVGLIYNEFFSMPTVIFGRSRFKCYHSDHSEILNDDGVTITNQIDPRDCRVKYGGVLMMPPDSAPVVFGMDAVWHGRKTELPYFNSIKMKMSILLGVTHMDFGILNSLFNNMYFRDNLSIFCEFIPQIIFLNSIFGYLCILIVVKWCSGKLTDLYHVMIYMFLSPGSGPEKKQDELIGGQGGLQLFLLLIAFFAVPWMLLPKPLVLKKRHEAMLAAKGHSIEMIQNYGALADDESRHRPHGGGHGHSGSEGHGGDHGGGHGDHGHGEFQFGEVMVHQMIHTIEFVLGAVSNTASYLRLWALSLAHSQLSGVFYDRVLMLTISMNNVGAMIVGFFVFACATLGVLMVMESLSAFLHALRLHWVEYQGKFYKGDGYKFTPFSFQTVKQLEESGTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA99</accession>
    <name>A0ABQ5SA99_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010752</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="176" mass="19418" checksum="792CBAEFE3764756" modified="2025-10-08" version="1">MEAATMAPSRDVSHQKLVCPRARKCRPDLSQSEQCQRSKLTSSLQAMATGGEQLGELELILQGQAPDAPNNYLGMSPPVRSGFSGGAANPIQHDSTWSACAFNASHSNLQALRELDMQHSFLRCTGDYRLLLGSPDMRSFMEEVAERSSQVQSQAQQESSRHLLPFTCRAQYFAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAC5</accession>
    <name>A0ABQ5SAC5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_010863</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000044">
        <property type="protein sequence ID" value="GLI66872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="17"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="367" mass="41490" checksum="402B7FB231F6C2DC" modified="2025-10-08" version="1">MAASAAEPDPLRETGRYERVQSLGKGAFGFVQLGRKVHNNELAAIKFLKRCDVNKYVESEILNHSMLRHPHVIQFKEVFLTSDYICIAMEYATGGSLFQYVQQQARLKEAVARWFFQQLVIGVDYCHKRGVANRDIKLENTLLQKVDGLPLPLLKICDFGYSKADMRSAAKSKVGTLTYMAPEVLVNRDGKYDGKVADIWSCGVMLYVMFYGRYPFEVPAGAAMPKATEILSMLDNMVRQRYELPTKVDISEPGKDLLKKMLLPDPKERITLEEVMKHPWFTTNLPPEAPTMNESYLKAGFPPGHQTTDEIKRILDEAKGRAGNSSTGSGRSGASDESYDNMVDNAIIEDLRTHRSAELQRYVKTYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAN0</accession>
    <name>A0ABQ5SAN0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000052">
        <property type="protein sequence ID" value="GLI66940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="216" mass="22472" checksum="56F48C4A7F1CE094" modified="2025-10-08" version="1" fragment="single">LRRHDTPDGSDMERQHRAAAVGLTSLGLLAGSSQGPPTAGKSSKATSGSDESSLGRETEDEVMAKETYRRPSGAPKKRMFGTGALEDLLPAIDEVDVHFDHELLDFMDLDEQQEQGPDATAVAAATAAAGGNSKGGGRSEGASGGVSRKSSHQAGSSARGGSGSEKEAADATPFFCLKETNGLATQVLPPQLFMSRNTKVVISIFRKQLAQAQSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAQ4</accession>
    <name>A0ABQ5SAQ4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="785"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="829"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="878"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="983"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="804"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="895"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1167" mass="120292" checksum="6A7CE6310C02F013" modified="2025-10-08" version="1" fragment="single">MASTDALLVASIDSAVVHDSEGNSNELTELQRVQVEQDMSKPSRPPPKVFASPRKRSEAQVERRPEAHAMPRSQSVKAKLRKALAPYLTSTYLQGEMAKHFTKEYLAAHPPPQNEDELPLYVQQHLSELNRVTENWKGCVGRALDPMMHEWEVDERLQLMRAREEGRLRAPAPSGQLDGVDRRLQGGEDDDIEDLDLDPDVDSGTLPAIEGGLTSTQIFGAVHLVSDVKVWQQILAAQKAEERRQHAIAELHIHHAARIIQRSWRDFINQKKEEKARLDALVSPDVQAMLSAAAYKAMRDARNKLAVQKLRKSLSRRFRGFGSTMPRDLDPIVRPPGSAQLPSPPLSPRRKRGSLGSNPTTDRPEGVDGAEGAGASRSASPAHARSLSRSSTSDSRYKSFAARSARIITRLVSMKDLVVDDRSRGGGREGARADGGSRVGEVAGTDGSTGDGQSGLVLQPPRRSRVPNVALPRRRPSSDVGQEGVNGDVPGPPRRSRLSSMALANKRPSADGAKGTGDQQGNGEGGGASAPNQQRRYRYARGANKKQSSPGASGVEVEGRKPDKSGDWAVHNGGLGDADVEEGYQDKDEGAEDEFGVDEEEDSDSVCASGSELDEGEAAEAAMAEAEEVVEVAAELAKAFLGAGLAGDDSEEASDLDDGAGGFGPHIGDVALEALSVMEAAATGGVGGKMGLLEPSQLQAEGESGQEGATLSGRVAPRVHRGRSMTENTAELRCLIAGALAAAASPLLGRKNALAVAQMGFGELLQLPGAKTLLPKAAAAAAAARDTRSSSQGQTAEVPLASFGRGGGGGGGRGPLEIRAAALPTSGLPVSGRAVGGRRPHASDSGAVPAGPMVPAATATVAAAAAVTQNKAGLASARKSVTGETSDGSAPFISPRQQPGRPSQTGGAVTLPAVHPGPTSSLTGDVVLESSETFAAIRRYSQPSVSVGTATGNGSGGGIPYGTHSPTSSPTGISAAAAAARATQPWASPRRIRHPPGAHTISSSLPDPRVGATQTSGPREPTWLLPPINRSSTGPAVLGGGVTAALGAANVVARVPSDPNLQRQVHLQHGVAGGGGGGGGVHMGGGPGAMAAMAAAAAVGRARASAVSSSLDSSLQPYGGHSGDLGPPVVVPQTVFIINSITASSVAQRARGGYGASPSEAAAHHQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAU2</accession>
    <name>A0ABQ5SAU2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046893754" evidence="1">
        <location>
            <begin position="26"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="379" mass="42188" checksum="3B9A109E26C9C26E" modified="2025-10-08" version="1">MAIIANSALLGGLLILAAAPHLVQSLESAGMSGSGSMRRNRGRPHHYALFTEKSPCAIMERRYVSSPLEQKWLQAAAKWKENGNGDLGDIGPYCTLLTETKEEIQRMLALIDALMNSTTSLSDAPDADAVLSYFHTIMQCGPYRFTQKEHIESLVAALRHPFSIKCPEGTFSQLPPLTNIFEKSYVLLASWAGPVQATKNKYYLDAGAGIGYRANANQHWMLETYEARGIKMDRALYWEAQSAQGPALLDGVPPDMFPAFAFYNIPAPENLTDGRNPLNVMAKIAKPGDFVSIKLDIDQPGIEDGWLRAILGNDSAAAGAFKSRYAQLIDELFWEHHFNFMPMRSCCWQGLVDANSHLVDSMKLFIKLRELGIRSHYWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB14</accession>
    <name>A0ABQ5SB14_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_010188</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="11"/>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF63">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI66396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="325" mass="37074" checksum="211D14AD7F345E90" modified="2025-10-08" version="1">MAIDRAPINTRIWSDGMHARFKQGIVSRIVAMITLFIYTGWMHILLALMVLAICRRTWAIIALISLYATLLLPPKPVLWGPFCRSKVFKTWREYFSFSYLFEEVLDPSKKYIFAEFPHGVFPMGPLLGATECQAMFPGFHIYGLAANVVFSVPFWRHFIAWLGSVPATTPHFKRVLKQGSVAVIVGGIAEMYMQDNRKEQIMLRDRKGFVRVAVKEGVDGGIVPVYHFGNSRVLDFGPQALSSLSRSMRAALGFLHGTWYLPLPRPRPIFMVVGQPIPVPRVPRDDPKYEEVINEVHKHVCDALQKLYDNHKTEYGWADRPLVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB46</accession>
    <name>A0ABQ5SB46_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Isocitrate dehydrogenase (NADP(+))</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="12510" checksum="37F68F9733960EE2" modified="2025-10-08" version="1" fragment="single">PYLPPHSPPPPPPPPPPPSPPPPPSPSPPPPPYLPPHSPPPPPPPPPPPSPPPPPSPSPPPPPHLPPHSPPPPPPPPPPPSPSPPPSPSPPPPPHLPPHSPPPPPPPPPPPPSPSPPPSPSPPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBV1</accession>
    <name>A0ABQ5SBV1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011618</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06227">
        <property type="entry name" value="M14-CPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034269">
        <property type="entry name" value="At5g42320_M14_CPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF119">
        <property type="entry name" value="OS02G0119300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045593757" description="Peptidase M14 domain-containing protein" evidence="8">
        <location>
            <begin position="25"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="9">
        <location>
            <begin position="25"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="397"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="482"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="493"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="818" mass="87251" checksum="ABAE9155C95DEE96" modified="2025-10-08" version="1">MSFLKAQAALAFLLIVARATTAHGQYSTLGDLLHWYAHSAQAHPSLARYTELKEPGFEHTMLPLATFTDHSDEARIRTKPSVLLVFGEHAREIITCEVGLWFSRVLVGDTAEIFSWAEWPEAFKPLRISPENIASTVQGWIRRILDNLVVKVLPVENVDGRQAWEAGNLCLRKTSKGVDLNRNYPFAFAAEPHHSEMYGGPHPFSEAQSRLIARIALDGGRVPKAYINVHSGEWAVYSGWDSKAAVGPGLPEDLSDLLIRSGDVCRCQAGPAGAVSNYLAFGTGMDFMYTQLGVPYALTYEVYGHGYGGLVPDDPGRIPEGTHNKPLNEYPYVTDGGIARSVPRVEDILQPLQHHQQHQHHHALDAYNLNLHEHRNAARQHQNRRQDRNRMSRAFASKLRRSADERVTEVEDLPGSRGGASAAAEEGAGANVAGGGKRQMAKGSGGFSGWWMDAASRVRKMMLGGGMTDAGGGVAAVEVTTTASGGGNDGRTLLQASSSAESSSSSVLSSSKFTSSPLSLPGSLGGRSMRRLAAAMKSGDGSNHAVTVPAANDVSTLASGAAASGAALGAARMARDLQADLSSFSGPDGPVVAMVRSQPGFHQGCFDMFNPPPGPAYRDVISRWVVILLMTLDHVADPSNPKPSPHPLPGTVLGGSGVGPWDVAGKGAESTAFSRRRLLEASTNTELLDDRSVGLGTGIDSDLAAKPEAGTKLTDTNEDVLGASQGMAAGEVPVISTEKAGNTRVGAGRDSYGSVDFDQRAQAAVYEVDPVMRQHSLVVFVALCCLGVVFVPWFLFVSNQITRPLPPSSRRSRQSLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC63</accession>
    <name>A0ABQ5SC63_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Elongator complex protein 3</fullName>
            <ecNumber evidence="16">2.3.1.311</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">tRNA uridine(34) acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">VaNZ11_011247</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ELP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.311" evidence="16"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="18">
        <location>
            <begin position="468"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GLI67058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="679" mass="74694" checksum="8D51F36175654041" modified="2025-10-08" version="1" fragment="single">YPNCPIRYEDTLHHNATVLLVYCCGQDLSLNGVVVSHLGAGVLMVAPEPTALHGKIDGNRAAQAVAAAVPSCDGGGGGASLGSDEARVRAIAELVSLLVAGVRGGGDVDLNQLKCEVSRRYKLARSPKLVEIIAALPEEHRAVLLPRLRAKPVRTASGIAVVAVMSKPHRCPHIATTGNICIYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYVQARGRVDQLRKLGHVVDKVEYILMGGTFMSLPSDYRDFFIRNLHDALSGHASSCVYEAVRYSEESRTKCIGLTIETRPDYCARSHLTDMLSYGCTRLEIGLQSIYEDVARDTNRGHTVEAVKQCFVGAKDAGFKVVVHMMPDLPGVGWERDIECFREFFENPDFRTDGLKLYPTLVIRGTGLYELWKRGLYRNYAPDKLVDLVARVLALVPPWVRVYRIQRDIPMPLVTAGVEKGNLRELALARMAALGLRCRDVRTREVGIQELHSNAAIRPDQVELLRRDYAANGGWETFLAYEDPRQDILVGLLRLRKLAGPDASRQPELVGRVSIVRELHVYGTAVAVHARDTTNFQHQGYGSLLMAEAERIAYFEHRSTKIAVISGVGTRHYYRKLGYELEGHYMVKQLLPPKSPVAAAPPPPPLVAAVLNDGDGKPLATASTVAGGLPVNRRRRTVARSTAVAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC77</accession>
    <name>A0ABQ5SC77_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_011683</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255">
        <property type="entry name" value="CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255:SF3">
        <property type="entry name" value="SULFITE EXPORTER TAUE_SAFE FAMILY PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="640"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="669"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="776"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="811"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="841"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="870"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="401"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="487"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="700"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="710"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="737"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="917" mass="94493" checksum="8ED04FBB35113DEC" modified="2025-10-08" version="1">MLLLIRDCLATSIPFPSPGVLLNVALPSWLLNILLLALLMLLVSQAVAKGRSLWVQETKRAQAAAEVAACHEIDAELLSANARAADLANANGTGIREALLVGGHHCALTHPALTGAASAGLPSLRIHHGHSYTCPRVPWGLEHDQDATYNYPESLENHVAVAVSRDQVRRSRGVSGGGSSVGAARRRTDDAIWDCMDGVNPAGDDDANCETPSSGVSMPPSAFPFAIHRVPSSNGADEVSGAGHLRSGLLGASPRGQSPASSIYAASWKAFNELGALEFETDLEGDSDFEGAGTAGSVVGRHMRSTRTSGFGSGTASPVLFPPSSYILSQLPRRLLPAHMQLSSATRSPHNALGRRTTRRRSFDQATGADLPAAAAAVIDEETVTAGGDSWLHRRSKWRSRAGHHYGDGGGDTDGAGTGLDEPLLGPQGEVPDRPHGAIPDPRADYSGGGAAAAGTAAAAGGDPGGGGFTAADPYRPTLRLATLHEESDDRVEGGTSGDGAGPQPAPAAPELPLQFPRTVVSTTAACPAQLHALPTSQPAADWDPLQLPCQQPAEAGSVILCDMHNGNGFLGGDVAADAVVLTRSSGRPIGYVYIECMSEVGPERRHMSGWCCAGMSVSAVIREWADGWATTFRRIPRRFAGCLVGAWVVYLALQGARGFQRKCSPAYWTLFAIQTVSMLAVSGGAMVMATTALSKHAEAAETSNGGVRVEGREGAPGGGEDRNGSAHRRQQQQQHGHVEEKESHRGGRGLPAAVAVEPALQCTAAMLVLKAPLSAFLATFGAGLTGGLLGLGGGMVMGPVLLQIGVQPQVTAASSGTMVLFSSSAALIQFAMLHRLNMPYAGAFAAASLAAGLVGTHVVAGAIKRSGRPSIVVLALAGVMAIALVCVAAFGLRQAAEQLRTGDLGFGGICTIAGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCE4</accession>
    <name>A0ABQ5SCE4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HNH nuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011806</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13391">
        <property type="entry name" value="HNH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH nuclease" evidence="2">
        <location>
            <begin position="162"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="363" mass="39622" checksum="3179C3D44ADA67D2" modified="2025-10-08" version="1">MALEDKAAAGARILELYRDDPDMGTKLFADIFHLDAAKFTDFRNMWMVAMGLPTEKQVLERILSGMAAGPGPGDMMSTLDMIRQEVKAAAELAANNNQLIRQEVKAAAELAANNNQLIRKLVRKYYDMSLTPRCSSSRYAACRRDALAYYTGSQEEPPSIVCAVSGVCLPAKEVNAGHIYQLRWIMLDHLDLDCNAPTNILFMQKKIEAAFDNFEITVLPVHHKVFLLKRSLSNHVAFEYRADADMTAAGGSTSSGKKAELKKVIWGELHQKKLSVAGINQPSDVALFVHAQRAFRFAVQRGWVKSSEVPALCYGDNEILRRFLADADSTSSGSGAGGDQGATTGSDLGESSQLPESEGVGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCV4</accession>
    <name>A0ABQ5SCV4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calnexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011107</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the calreticulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF1">
        <property type="entry name" value="CALNEXIN 14D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044972608" description="Calnexin" evidence="8">
        <location>
            <begin position="25"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="424"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="520"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="521"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="547"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="582"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="595" mass="65665" checksum="517C346A24E3891B" modified="2025-10-08" version="1" fragment="single">MSTLWHSYALLGLLLYALSRQSLAHSPTVIDTVRSQSLFFETFDDDGWQSRWSHSRDNKYNGRFTTEAVKEWTDKGLKIPEKNKFYGLAALLDAPVDPVVASSPTPLPLVVQYEVKYEDGVTCGGSYLKLLTADPGFTPEGLVDSTPYSIMFGPDRCGATSKVHLILRHRNPVNGTITEKHLASPPAPETNDFTHLYTLKIFPDHKYEILVDGVMKSSGSLLDPATLQPPLLPPRELPDPDDKKPADWVDLAEIPDPDAIKPDNWDEREMIEDEKAVRPRGWLDNEPVLINDPAAKRPEEWDEEEDGVWEPKQIPNPKCKVGCGPWTRPMKRNPAFKGAWKPPLIKNPAYKGPWVQKTLPNPDFFDVEEPIRSGVGAIGAVALELWTIDSGYIFDNILITRDPAVAEKAFKELWKPRHDAEVKASEAKLKEKEQKEKERRKEEEAKKKNARRGGRSGIGETALSNAFLALFEDDGWLAPAAPYMGPLLDFVRENPDALILSLVVGPMLLLTVILLLRGTGSRKTQPPSTHAPTAAVEEEKPAGGPDAEPVVEAAAGRAADAEPSSTPAAEAAEGPEVVPEGDATDGEIEEEAEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD15</accession>
    <name>A0ABQ5SD15_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carbohydrate binding module family 25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="54091" checksum="4A5B0DD767A8EB8A" modified="2025-10-08" version="1">MVAFSSSNKAAVGHTLRGRQPQLYVHVVTSGTRPTVLSGAMVVGRPSGGPSSAKSPQRAFSVMQNAMKIPGLASALHLIGMYGPGGSNASTDSTVAAAGGPSTNGGGMTPAPDDRPSPLKANYDTSSQQQQSQPTSSYRGGGNDPIEAEIIAASAAGATDASATAKLVSVHETAATSTADNHGNRHLAQLNTPVQHLPAQMEAASAVNSLANGTIHRHEGVEEVLGSVDPGSRAENNITPYTTPLPSLAAQIREIASTLRHINSLRLEYLDKISQQELELAAKDQVLRSREERIQSLEHEAVELRRSLALLHSAKSAAEVEVQRLRSWDSKVATSPSSPAPNATLTAAAAALQPAQSAAAAVAAAASAPFPVPEAAPPMPRPLTSPPAAIAAPACATLAACPPASMPPVSTPDSTPAPPPTARVLDIVLSYRSSWPQAFLHCNVEGKGWTLVPGLRMENAGPKEYSIRVQGRSLEFVLTNGHGEWDSPGGGGIGRNYHIDTPGDYRLHYGVLTQVKPWQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEM3</accession>
    <name>A0ABQ5SEM3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_012261</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01635">
        <property type="entry name" value="Glycosyltransferase_GTB-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF376">
        <property type="entry name" value="EXOSTOSIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="206"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="699" mass="79564" checksum="97621A10CA47F3C4" modified="2025-10-08" version="1">MTHLPMFDLRALEGHTAAAYSHFRQDRAWTILLLIYALIICTYVHGLKPAPRFLRHVGPRCHENCTKNGNCNGDTGKCDCSFGYEGPACESRSMPACHTSQKTRSPPQYGTWSLKNCNCFRQLAQIGCPPLGDRECLYQALWNVSLACFEVDGVPPEQQTSDVPRDGFSHPSLRWYWGQPLLGREAMNETSPPKQHLLPNGMYQLPLDACPKRCNERGICGGGSPSDMQGTCICMASYKGKSCETAAQEDCYLSCSGGSRAGLPSGTCIQGFCHCPPGRWGLACTRDEYYTSNVGWKPSYASLRIFVYDLPQNVVHMRTDDGSGQTQDAMYLAEQAFLSQLLADSASSSSSSVVTQNPWEANLFMVPTWSYWYTSNTVTPVRLFRHVIQYLRTNFPYWNELGGRNHVLWAVNDRGSCDWHHLESDLHHPILVTHFGQAARLPPLHVMLNTETLEALERRRQSIRKVQQMLTDFREFNHVDLYYENMPCYRPEKDVVVVPIAPGWVYPVIHTVYGKDHQQYNRSMSRPYTFFFSGGIRTEMMYSQGVRQAIAKMQQERSTWNRPDFLIRTAGFPRASMMQSKFCLAPSGWGWGIRLVEAMAAGCVPVIVQDGIYQYLWDVVPYDEFAVRVSRRSLHRLPQILDAITPEQLEALQAGLTRWHRAFLYGTVHPDGLAYNFTLQSLRRRLTNMWSAHYRRSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEW0</accession>
    <name>A0ABQ5SEW0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="114" mass="12593" checksum="D3D610176F99DF6E" modified="2025-10-08" version="1">MMVLAANAARIHQKEKENKAARRRQAKSSQPSKARNSSRNAMNSSKAAAKGYSPFILPSRFSNDDDVRPSTKVRRTSTGIKRKVPVEDNSTDGDDNNDSYDHQGKKVKSASEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFA1</accession>
    <name>A0ABQ5SFA1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039715">
        <property type="entry name" value="ZCCHC10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13491">
        <property type="entry name" value="ZCCHC10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13491:SF0">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="686" mass="72118" checksum="211C9130B738824E" modified="2025-10-08" version="1" fragment="single">RVRNGSLFQDLNRASGFNHTDLVLLTGDTTADVPILQLQLQFLNDTLISGLDTSVGPFPGLYLRPQPGGVLLSTNVTVTFQYLNIVNVVANFKLSSSVSSNSDRSSSNSSSNNGSRSNSGSSGFNGDGTTNNSGSGATGTANWSLSLDSFSLSPGSLLRLVRSRVVLQDCQVAQRLYDAASGQYQPQPPGFNITQRDVVREVLANRVQLPQLELRDVIIGCNEVCGMTGTRTLTVNSQTDWYTAMSVINRHADGCNGYIVELATNVSLVPSPTLQPPSSPYPQQSSSTASSRFLVEATGGSGGSGMPYKPVSVQVNLTLRGVAPGSSSSTSSPSSSSSSSSSTPPPSSGAVVLDAALMRSVFLVQPPARISLEQITLINLAAPQTGVLAIPMYFVARPDPYVTSPQSVILTNVTLVLASDELSYEVSWFRSLADGSNLMAPVMMGWLADVVQVAKVSSFTATSFTLSRFIGFGIDAANLVITSAPPAPFTLSQRTIGISLFAPSPLDSYPQILGVNSTAALVSALNASAGDLTSQPGLFEAYPGVPNLAAVSLYSYNHTAPSVFAPVSYGRVVLQRSYIVGPYVNVISVAAAAGGVLPPPVLDFGHTRSTWAVWNGSDFSLTLRNLTLVGLGSPSRWPSDRLVRCLDLPVWALEGSRVPSLSGDPPALILDGATVSVSPQELQIWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFI7</accession>
    <name>A0ABQ5SFI7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="160" mass="17086" checksum="B9862E54222718ED" modified="2025-10-08" version="1" fragment="single">VNHDFQSELCGSYFCDVDHFFCTSLKRRLLTTEKRISSYSGSRNPSASCLYFKVSAMASFGNAGTASSASSSCRRNDDSPCSPRPAESGSTSAASSSVLPRLLLLSALLPFSVRADDWHTGRATFYGYDGGASIDQGSCMYGPLPNYMVSTGRNIGALSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFQ3</accession>
    <name>A0ABQ5SFQ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nuclear pore complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013082</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nuclear pore complex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the nucleoporin Nup84/Nup107 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007252">
        <property type="entry name" value="Nup84/Nup107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13003:SF2">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13003">
        <property type="entry name" value="NUP107-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04121">
        <property type="entry name" value="Nup84_Nup100"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5 7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="600"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1170" mass="125778" checksum="C0849B88751E0E84" modified="2025-10-08" version="1">MNLIGMDIDAPVVVPPQQRQTLEDALANVFGALLETDLSDGSTISDIANICTEMANELRSLATEQLHRPARYMALMTQADEMDAHAATWTLLFYLYGTSLATLTPAGAGGPSLAYGSRKWLYRQLLADHIKKEIDADVARCARVVSWLESTADEALKRQANVPFPPGEGLWLETKMEMRTRAGALVSELDPDAPGRMGRQLHPSNAKSQERILARVWQLLRAGELQAAQEVCDNVGQPWRAAALSGGGLYGVLPVGQIAEDYDNGPVVHLQKEELSDEVANGRGPLLALWRWAARQAAAAGAGDKFERAVFGALGGDAAAGLPVCVTWEDFAWVYCRSWLESWAMRSTPPEAQPTADLVSGDAVMAALVRCGVAQDVAKNSVEGAMSVVTSGSGGGGGRAYITDILDDAAAARGFGVLFAQHLMAAEAMANAGPAAVQAVRQIQALLIRGDMDVLLADLCRMIESGHRDGGAVGDAGGSVGGIMTEGGYSGSALFGSSGGADLERRQRICALAAHMLLALGGLGELVPQTNPDMVFLQAQVNVKKAHRVLELYTRALLAQEKLTLVPRYLCVLPGHVRNSLCSELLELYTKHMLDVDTSAPPRASDGVSVERADVSRQHLHHGTTTSAATTAASALAEADAACSHIVAELGMWFQRCLLLQKHHAGYSVNVSAGAAAAEGPWCGYQWTDVSENELRNQLAFFTLGCRYNREYGPYQRARVARWLVQPHIVATAAAQARQGNNPGSIHDADGVVDADIKTDADRDGMNGEEDTARWIAANVDPMSYHDLLDFVNCLCCEFALGDDVTAAAGQLLFTEVLPQGLELAVISAVLILEEELDKAHQTAATYLHEEGEAEAAAAEAAKKKHLADRLRNLLSELEEWALYYDLNAKLSAWSERHSACSRAAPGEWGSSEAEMLRDGGELLRELLTRLLKPTWQQELAASERAFAAAEDGRVQLSLTLTCPSTSTHDSARDPYPELQGASLHQFSYDLQRGLLRSTADRELEMGMEVVPAVGGRGHVTLNLTVASEAKLWRGLVDLVCTLLRGELEGVPPVLVVTLDADRATSLAVCRRCRIGQIVRRCAALRLSLVGLGLDAASEDAGSQLVEILAEPAGDSGANLLDSHMLELLSPVQLAQVLQMEALTYERHLKNVQERRRQLPQKQGEAGIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFX1</accession>
    <name>A0ABQ5SFX1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012594</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="164" mass="17970" checksum="75AF8DB82020447A" modified="2025-10-08" version="1">MTMYLYKPFGDNLNSSEFGITGFRNTSFSNTGLKSASLSANYMPASHCSLLSSSPMTFSAGNPNNGALVLYHPNSLHGLYYDEHVHGPLATGRIVNPFTPGLSPLSSVAKPNYAKDDALRERFPAVRAADDPLNWITDEELDMRTSRAAASQQRTPLRCQSMHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFZ1</accession>
    <name>A0ABQ5SFZ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053248">
        <property type="entry name" value="Zinc_finger_MYND_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46533">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46533:SF1">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="41066" checksum="A4D5A251B18BC8B7" modified="2025-10-08" version="1">MATLTYTVFSLGEAQLHQLHTGNGKLFVMGEVAVELFQESPTAFLQELRKNKLPKLQSANRDVLHTVAELHLPVESSANSQGVCLLPASTVETLLIDKRRMELVQPFKLALLKLASQEAARLMAAGEYELALPVALDAVQQGQALFKPAPALQLFPLYLLAAQANLGLRRAKQCEDFLALASWLAMKEPGLTTSIMKSQLSRLYGQLYAFQSKHAEALHAFAEDVYYCSLEYGPEDVRTSLGYYNMGKVFQSAGDIEKAASCNDQVVAIWAATLNAVVLGLTEAGGAGRPAGAAALPVGRLQLMEVVDMLADITRTRAAAVGSGHVTVGEAHLVTALALIQLEERARATEELEAAAATFGSDDAERMRLVDMARIMLKALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG81</accession>
    <name>A0ABQ5SG81_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-1-gamma C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012874</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1010">
        <property type="entry name" value="Translation elongation factor EF1B, gamma chain, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001662">
        <property type="entry name" value="EF1B_G_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036433">
        <property type="entry name" value="EF1B_G_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00647">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01183">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89942">
        <property type="entry name" value="eEF1-gamma domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-1-gamma C-terminal" evidence="2">
        <location>
            <begin position="247"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="406" mass="43151" checksum="71D497FAFC9B3C97" modified="2025-10-08" version="1">MGCGASVHAQGTLPLPSESEREAVQTQTKNSTPAPLANDIQGAQDPDVKSDVDKDGAAQLTAGLPTPAAGAEPDAEQQSQKETCTSATTTTPTEPGITNVQDQDAFSPVVPCSASDVPSLGTGTEEGLVDNPRSPSSPRLSPAPQSAQRVRTAPGGGGGVQEATRLVLGSSTASSAMDLERPTPCFTPGMSSGVFDPAVTPSSATATVMLLPEAPPGAAPRFRYTDASDLSISSHSFDLDGLGGDDSDDDEVATLFTLQSWKRYYGYNKHKHNDLVTYFWRRFPPNDISCFCISYRNQELLLTPYMAENCIHGYCARLEDLGLASEVFLQMYALHDRACDQYRIVGLMLVAGQSLPAALSSLSAFDGFEYHKTETSRVLVKQFVSALLVGRSPLHSLTLISGREVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG85</accession>
    <name>A0ABQ5SG85_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pyridoxal kinase</fullName>
            <ecNumber evidence="2">2.7.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013421</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="8">
        <location>
            <begin position="177"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="449" mass="47967" checksum="E8AD36EE21A7B29C" modified="2025-10-08" version="1" fragment="single">MSESGTVIDIHVLNQSEEPCCLGDKARMPLEILKRTIRSRPGAITFKPKLLSRRFKDFASSLAGQGGPAPLSPPSPSPSEPRLQILPWHAAMESGQPLPRVLSVQSHVVHGYVGNKCAVFPLQILGLEVDPIYSVQFSNHTGYPTFKGSVFDGEQLRALVSGLEANGLLSHTHLLTGYIGSLSLLQAIADLCSQLKKHSPSLTYVCDPVLGDEGRLYVARELVEAYANSIVPLASVLVPNQFEAELLTAGTPITSLNDALAACEMLMDRGPHTVIITSMALPGDPDGIMLVAATRLPQQPDGRLHGATRLKMRIDRIKAYFTGTGDLFAALLLAWMHHHPGDLATAVEKAVGGLQAVLADTVRHCGPAPLAAERTAEVCALRELRLIPCQGKLQDPPIRYRCEVATSDVEAGPAAGQGEEHEGVPRGPDVPVAMNAVDTCTVTASRPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHC4</accession>
    <name>A0ABQ5SHC4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTP-binding nuclear protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_013748</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Protein transport. Probably involved in vesicular traffic.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the small GTPase superfamily. Ran family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00877">
        <property type="entry name" value="Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00627">
        <property type="entry name" value="GTPRANTC4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51418">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="223" mass="25351" checksum="E47555795AFCF64D" modified="2025-10-08" version="1">MALPGQQTPEGVPSFKLVLVGDGGTGKTTFVKRHVTGEFEKKYEPTIGVEVRPLDFSTNRGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTSRLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKPKQVTFHRKKNLQYYEISAKSNYNYEKPFLYLARKLTGDPHLTFVEEVALPPPEVNIDLAEQQRNEAELEAAAQAPLPADDDDELLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHG6</accession>
    <name>A0ABQ5SHG6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013459</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019537">
        <property type="entry name" value="TMEM65"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21706">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21706:SF15">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10507">
        <property type="entry name" value="TMEM65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="31076" checksum="B4CDFC4C02D39713" modified="2025-10-08" version="1">MLIRSLQQHRRALLGPLMTALQDTALSPCCSGKEYPCSGMCFSQDGLSMDFSMLPSCSGRQHGWRYCSTNSSSEQTGNGLEEAFERDPHSAAQALAAKLSPAQRAVLVAALAQHTETQQIDDAYWEELFQAHDVKGDSPGALSRAEFQAAMLAHTSLIENRNKAPPSSAALKMVFLASAIPFIGFGFLDNAIMLVAGEEIDDLFGARLGLSTLASAGLGNAVADVIGVGAAKYIEQLVRMMPFVKEPKLSKTQQAMPRAQRAKLAGAMTGVLIGCTLGLTPLFISGSFFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHH4</accession>
    <name>A0ABQ5SHH4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="273" mass="28886" checksum="9509C1A9C8FF5F4F" modified="2025-10-08" version="1">MSKIYKDLSDLVNVQFLGAQQRSEDFFSFRLQIMEAWSTSHINGAGPRRRNSLELSSIPFVVLLFLVIPLPVYGQGTQACGGNTGTPCPSPVPSSSSPPSPLLSASPSPPSPKSSSSPPPPASCVSGTHRRGRLLLKGGGGGSYHGSSSSGGSGNGCSKLTKTDIIIIATVIPTGSLLLVAAVIVVVIIYRRRKKARQQVQPYTGDPASKPWTAGSEQSTPPYQQPQASYQQSSVTHQTPDGPIKQPPVICPTPSGPYQQQYPQYQPNPPITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHH6</accession>
    <name>A0ABQ5SHH6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="474"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="556"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="685"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="712"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="945"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="903"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="592"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="750"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="771"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="812"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1669" mass="175255" checksum="B4488ECF4F5E9EA5" modified="2025-10-08" version="1">MATAVGHGHGSPVGAVEATSGTSAAAALPAIGVSGLPAGAGGTISPLGRERSSLTSLGGGPPRKETSYASSTASKISIATLPLDTLEHIFDDFRAHQHHGAKNHANGGNRGGRGGGGPAVRISTTIAAGGGGGDGGRSSVRTWNPVPGGGGGVGTAGPNRRSHGNVGSLSLHFAAKPTTPPGNDPGGVLPGVLMQAPVLPGLPPGAPLSLSSRAAAVLQVPESAAHLQRFIALDSAQLENHFIIANPNGATVTDLQEVIDNASSIDGLPSGREAVVVLTTTADGVPVPRIVPLDQPAPGGLRRAGMGHARGASATGLGTVDALLLDGAVRSSNSSASLLVGGQGYLDMVDISAGYEGGGGGGGGTEGDAVTPTNAGNGSFVRAGSNPILVPVSPLSPQRSMAALYSPRRGGTPPARRSDNGMSRGPPYTSTVRMSSVTMLGPGRPSSPPLRSTTGIAIQGNNLQYNTLPALGDGGGAAGARRSAPRRRGSASVLSRREASNDAVTQSLPVLVRTISPDRSNLAAAIVTADVGFGSLDGGVDSMAASPINSCSVHPGGGGAGAGASTRPRSSAVDGPQELSPTRSRLHARIVTLQTERSSNQLMQSAQVQRQPSPLQPRGGRSGASRSTIIMSGLSGMELVRAALKQVETGERTLVDPMVRISSNGAAEGSRRASHSSVTTACSGLGSGVGGGSEVSQSGASPVPVLDTAALAEETAREEEEEREATERRQARLVKSLTMLLTTGRKGGRVSRPSSAASRLSRRSSILLAQDADDADDEDVGSDVSSFSNGEDEDLKYRGGDGHSQQPSGDGSTSVSSQPPQPSRESSQQHLRVPKSATFKHDHRRYNRFRSRLHAGSRQPSEMPHLGVHARSVRSSCESDSDSDTLEAAVAIAADGGLDPKALVEFAQQLDKANEEFRLVEAARDRARRLEALLTQAQLAVTNPAAAKRRATGDGSTAGAGGAGRAASPDKQPIDLYLLAKRREGNKPHNVTQVISALEAPMDRPMEVDLDRLLNSIGDALAADEQDVKATRFDETFRIRTERYLAQLCRMNPAAFEEVSAAQAEADYRSSVERAKLRLRQLHGEVAGEPLTSAGKPRSQYDKAIVMLQAHRPFFLPPVIQREVPERAPVPPMIGGRGSSRPPWDVYASIFRERKRGDARDVYDNDAVLAAQFHLDWSRTVTKMLFMKLVAREDLGVRGKSGRQALEQELAEVRSTLAEHFGMIRSAFTYYSIATPEEVYAATLSAAVAAAASTGGSIEVAAAEIAANFTAAAAKRGPPAPAAMRTSTSGAATIGVGRTVSPVTSSRDAAVVAEKPPSLAESEHAMILAAATAAAYTAGGATGEGHHILEMDEQRWLAFCTSAGITGRAAGVRVSDMRTLFWTVNREEEKNMTSESLANNDYAFMRFEFTEALVRAAFGRYITAGKIRDASDATRRLMEDLKMSLPPIARVDPNNFRRNRLYTAEMEVAVVHNLELLTASFKLYKARDRAKYLGVDHWMSFLETNNLMAHHMGGITPTEARLMFAWSQMVVVDELKLRRRAVSLQLWDFVEAVARLADRVALPTTEEMDKWMLYNLNIGRHTPLSPGHTRVWTYCNALGTGRGGAGLERRPSGREILSAPTRPLAIKFRALMEYLAGSLAVAWGGNNTHETAQSMLRTANMLCGGVELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHM6</accession>
    <name>A0ABQ5SHM6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="553"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="567"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="895" mass="94668" checksum="AE5B31891D43D28F" modified="2025-10-08" version="1">MIAAILPMSIPILATNQRSAVRTSILAGHVHRYKHSFAQLMRPHQAGWHRRCQLVSFISCLLMSFSVLMLLETGYSAAAPVAAGAPTTTATPLSMQPSSVGPSGTSTNGIAGSIPNMSPAPSADLGSPSCSIQLQSGISQPVLSGPYYGIFPSNVGLPSSSAGSNCNCTSSPLTLVWGKAEKIGNRLFYALRLQLTQPAPPSSAPRGQLSQLIIQMDPKFAARCYKVNSTMGRQPFKDKLLFRPTVPWSYRDPAGIPVNAFIGYSSALDYLELSLDITELRREVMNATGLPQDGGLTTEALTRGVFLLGRCLSKSAKNLCNSWRIRDVYTDTCITAYVWEQPAPPPPPPSPPSPPPPNPPPSPKPPPSPPPPLARKPPSPRPPPKGIAGGGGITASETQGTQPPYTSRWSSPKSKARRLGTAVDVGNSSAAQSLSLPAATSGLVTASPYVMVCFSVLALGRSLLVEAPPPPPPQAPPSPLSQPAEAPPLPTPPTPLPLLRSPSVAHLQPLGLPPSRLPSPPASSATQPPSPLVLSPPPVSRSPSPSPFPPRPSLTLTSPPVSTATRPPSPSPTRPGPPPPPSPPPTPPPPPLPSPPSPPPLPRPPPSPPRPPPPALPSPRPLPPSPRPPPPFRPSPLPPRKPPSQPPPRPSSPRPIPSPKVLSPPPIPQLPTTIQVQKGLAGAPPFLEVRDGVEYAQCSVTSDGMIFADDEQFPYGVFHSTVCSCGAAPYGLRFGADITFKDITYHTFRLYNKSCDEGAVAMGANVCNTIRPVINKIAFRMESDAAICYNQTGSMTFIENATNFRGVRFWQDGASVPGSWLPARVGITADGSHVELFIYGLKYSYTDGMFLLTKCGAKGSFNRVCRRTTEGMCLFAFVDTPEHRFATICKAQGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SID4</accession>
    <name>A0ABQ5SID4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_014038</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007199">
        <property type="entry name" value="Rep_factor-A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04057">
        <property type="entry name" value="Rep-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication factor-A protein 1 N-terminal" evidence="8">
        <location>
            <begin position="6"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="7">
        <location>
            <begin position="199"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="10">
        <location>
            <begin position="332"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="9">
        <location>
            <begin position="496"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI69429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="645" mass="69561" checksum="6DE32FEB497444A0" modified="2025-10-08" version="1">MATATLTPGDVLRIKEKHDFANGVVLRVSGLQEVNGRKHRCILSDGSANIRGILASQFADLVGSGELSNGCLVKIKTFVTNSVGNEDVLLVTDLTVAAPGNGSQPMEADIALTCHNATPEASSKLAEGKTAGLGHWAKENSTPMLSPPTDFKANKTPGQSGPTGRTPMSLTPMVTPLSISMEKTSLTPPSLRISDRKSYHKIAQLHPYDSNWCIKAKLERKAPLRSMNIRGSDVKILTVDLLDDTGQMVQGTFWRGAAERFAEQMVEGKVYVIHKFKVKPADKKYSSVKADYQIDFTDSTDVSEAADQDTSAMNAAVEITPIDLLPRRVGARAPVDVMGVVLALGSLGTVKRKADSSELPRRDVTLGDMSCKSVVVTLWGDNAHAFAAQLEGQEGKAVLQVTHVRVTDFNGCSVSSLTKSVVTLNPAGHAATQLLTWYSTADMSPDRFTPVGQDLPGTRGISNGNGAVPSRERFFTLKDVAGVTPESLLDDKANFQAVTACVAMINSEQQMYYLANPENGRKVVDQGGGQYAEADGRVVERPEHRYVLSVKLADHTGEAVVQLFNKEAEAVVGLKADELAALKDAGSGFAAALRAAQWRPWSVVVMSKAREYNGERRVRHTAHKVDTLDWVTEGQRLIALIGKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIL1</accession>
    <name>A0ABQ5SIL1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RimM N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013756</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.60">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00014">
        <property type="entry name" value="Ribosome_mat_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056792">
        <property type="entry name" value="PRC_RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011961">
        <property type="entry name" value="RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002676">
        <property type="entry name" value="RimM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036976">
        <property type="entry name" value="RimM_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02273">
        <property type="entry name" value="16S_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33692">
        <property type="entry name" value="RIBOSOME MATURATION FACTOR RIMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33692:SF1">
        <property type="entry name" value="RIBOSOME MATURATION FACTOR RIMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24986">
        <property type="entry name" value="PRC_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01782">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="RimM N-terminal" evidence="6">
        <location>
            <begin position="95"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation factor RimM PRC barrel" evidence="7">
        <location>
            <begin position="208"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="317"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="41848" checksum="DE494A5726375973" modified="2025-10-08" version="1">MALGQQLLQRLAGESSLGRRRVYPLPTSHTQRVPHIAGRLNTPCTLWGRNCVGSTLSLAATAGSRASTPSAATQQQPGHGLVGTLPFNENEYVEVGQIVGAHGVRGALRLELSTDQPAERFRAGRRLYLLPPSARGRDNRTEPQEPVLIAVTAQGAKMRPMGKGEETWMVDLEEVTTRTQAEQLRGYVLYCAAADRGALRDPDEFYVTDIIGCTVTDQVTRRMLGLVVDVYGGTGTHDTLRVKLRANEEDILNSRIRYCMIPFAKAICPVLDLSKRTLEVAPPEGLIELVTEEKLMKPLSDAVKAQKLAQLRELQAQEALLQPSPQPQKTEGDEGEDEEDGHGHEEWTQQGGEGFAGVRRGRRPALRKRPGSLRLGRRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJV3</accession>
    <name>A0ABQ5SJV3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="106" mass="11565" checksum="261D889637FB2138" modified="2025-10-08" version="1">MVLDRDARRVVQVLVPGMPPPVLDYVFASVTKANARPAPQSSSPHPPRYTLRRCDGGSLGELSILDMAELALAKDSCRSAFGQRDALAFHYRDAAEPRIMADNGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKC2</accession>
    <name>A0ABQ5SKC2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014521</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000088">
        <property type="protein sequence ID" value="GLI69817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="529"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="688"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="785"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1203"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1276"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1062"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1415"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="692"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="791"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="818"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="849"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="966"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="978"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="997"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1299"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1653" mass="169338" checksum="FC37B99CEC488288" modified="2025-10-08" version="1" fragment="single">RERHNSSSTALEERAARLVTELDAARRREAEVLRVLAASRQAGHVQQSGQGPSVVPAADSTGTCLSPVRTPFTAVSTGAVPTTAEPSIGSADSWSDGQVLQWPASAHVSTSALGSRRNSSTGATGTGAGVPVAVSLAVSTTAPGDESPLSGGPSLDAGGVQRGARDAGEREDVWGEFVCAPQGATGQWSGTKIVRPTPGSSAAAGTGTVAAGCGSAAAASGPGFISEPATSVSTDTEYNSDTEFYVDDEDDEEHRGRALAAALAKSPRGSLVELPVQLPPLLQEAQLQRRRPLSPGAVVADTRLIHRPDRDSYISVMTGAGRTPAPSFVIDAGNGKSAETKSNLSASDTTEASVVDDLEATFWGVFPVVDMKVNPSGALGLATAAVAAQPAREPAVAVATAAPPADVLGAVDLEATLSGNFPVLPQLLSTLQHHLPSLLPAEDGKVNKEPGAVDGGTYHCAVLALPATAVPFSFPGPQGSAPEWDGTSFCSGNQLAVLSNQEQEERSRSAVVEVPKELNNLASSFDFSPGAQLGSRISPPAPAQQAEPADSSMAPSDSMEGQVLRGRVEDSASTKAQIVDAGVSYAEDKNGLGDWGGSATSSVICSLPPLPHIQAAIQSPNRQVWVDLTDDGESSIVEDYDDDGAKDSAFGSGTQDGSIWPPAPAAARAAAGITPLLVPNPLFNETDEVSPPWSTAEWSTGRSPVLNNEVQTEGASLPSTPGSETRECSASSMSSDYLADCNTVGLTAAGAISYEAWNTLYDMPEEHSSPDSEARGFFHEQAVDRCHSSENARSGNPSCSQGSRSRSVESGETTPQQRRVEPGGDECRSCGGGGAQRALEAGQQTDEETGGRKEERQSSHGVSDSDEDGVRSLLAAGATLSNSERRRRQRRRMGDAPGGPPLEHPIDLLRQGWEFSGGVASPISPRSTPQKLTPADAPWQESPTRHTAPATASPNDKGSQREEQQLEQLLPLHALQRGRSSPSAVRRSGISTPSPRASPLSKSPVSSTSALGRTGGSTGAASSRRYTDMPALEASPGALSAFSCTTPPSAVVSRPLSPLEVRQRSRALLGRARSRVAELEDQCRALAASLHPSPIDDTAVAAFSPRGVGLPVTSSQQATPGLLWRSGVADQPGASSGNPGVCFEQCESKVVEETLGNGGSSCSSIALVATQPPAAPAEVVDALEDLRGDLNELEALHRLASRATKHHHRAEGISPSALTSPEPHPAGQLLAGLAGGAVATAVAPTGRAHVGSPSPVNRRARLLLCLQQAGSPQIGLGSPGIHAASSSSQVEDVDGGATHTPQHNTNTDQCATDPQSSTEPQSATEPQSTGPQPARLIATAADPSARVMPESGAVAAVGPQAAAPLPHDGCSWQVRDSASIVAAALPPAAPSPVPSVPGASSLDPALVALLQLSIQAQVQGQLGQLEAKIAELQRQNEALLAAATAASTPATPLPPHFRYSAQFGPTNTTPLPLSPPTAVFDASRGSGAVADANMDGRGQPIAGDTPAMSWSGSLDGLWLRGGRGVGVNSSADQLRTLQEAWPVIHAAWESTPSPPPSVVTASGGSQGRVPASAVSLALGTVPTAQPLVTAATSVTPADAGRKVSASAEVVAGPFSPEELSQLPPTVQLFAPPQLRAAALAAVREQAQANRHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKL6</accession>
    <name>A0ABQ5SKL6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="146" mass="15894" checksum="2D8EBDF27C44F612" modified="2025-10-08" version="1">MSSLIPSGAKSLVSSRTTRPLTVVVHASASGAISHSGQGGFRRPESNRNVCGVFQLSGSPVSLSLNDVRDAYEACSTARTAAERCQCFEVYGLDADKVDRYYERVCEMERLIDQASSGICRINGDHNSTPRRLASNYDRGHHRPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKU6</accession>
    <name>A0ABQ5SKU6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014502</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14505">
        <property type="entry name" value="CDKN3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057023">
        <property type="entry name" value="PTP-SAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22784">
        <property type="entry name" value="PTP-SAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="140"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="270" mass="28991" checksum="C4B4BE4941C05724" modified="2025-10-08" version="1" fragment="single">ENLNILMQRNLQFQSSRFGGSSDLSASTFNGQPTTVNSESHPITVSWIPPCEIAAGGRLGLTFCPGKHISARDGTQYCRDLRTDLARLRLVHGVHTIVCLLPEAELRYLKVRSYAAAVEEHGMEYLHLPIIEMAAPRDVSQAASVVDAVADRLQEGRTVVMHCKAGVGRAGMMAACVLVRLGVRVGPAEAIAAVRQHRRGAVESRRQEEFVAVYCSTFECRNRTKNAMSAATVQGPHAVMTLPSGGWASNVNSGAHGKMVPGDSKSAGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKY4</accession>
    <name>A0ABQ5SKY4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014972</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046653209" description="Peptidase M11 gametolysin domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="3">
        <location>
            <begin position="138"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1103" mass="116126" checksum="75B411246D98DD59" modified="2025-10-08" version="1">MRINLSFVALALIATSFGVVVHGQTTAGSVPPVAYGPIAPAAVRQTILGELLYVTVDNTTDQWLIRNSDGNLVPLVGGYKPPPTDIFGVKISSGSVVQLDCVYNPLTGDCTPDGATSVKVLQAAQADVVNGVNITQHLLVIILDYPACGLPSNISEADVRSIYLGPNLDGNGGMAQKYRQCSYGKFNFNFSTFQVVRVPHMCSSAITTSCAYWAIQQLADTATKARIGTGNFSSFTHYTYLVPPGLSSVCTWGGLALLPGRQTWLQTVANRGIYRWRTIMQEAIHNYGLWHSWRDGVEYADSSTAMGRGEACPNAAEISRMGWATAAVGGNQLDKDILLPGSAKTFILPATYLTGDNNYLRVLTNWLPTYSNSSLGRNLYIAIRAAKEGDAALGAEFASKINVHEVYAFTDNSPFYIYSERQITFINSTGALSQLTLIPYNLVVYGGAWVAIDTMRVHLCRYVTSPSECPALSSLETVNPPPPSPMPPSPRPPPPPPPKPPSPNPPPSTRPLPPNPPPRPPPPPSPKPPPPPSPSPPPSPSPPPSPSPPPSPSPPPRPPPPLSPSPPPSPSPPPSPSPPPSPSPPPRPSPPPSPSPPNRPPPSRPSPPPSALPTPPNRSPPPPSPPLPNRPLPPSPLPSPSTSLAGCPVVAGFLGPFNVTDHLGDDMLSSADPISDCTLRPNCLGVNSKGLLKSKTSPTTSVSGVCLYLRNATFFDSASCGAMLEVYGVIPGASWGQLANAAGSQTIFTNANCNIRACTYYQTKYGTNNSTSWGSLPTSLQTSWTKYGCNSVVLKCPAIPGYMATPDADHTGDDIANAGSASAAAALCASNPSCQAFNNAGVMKSKAYPVNAAAATAGSCLYTKIQGYVWPPSPPQSAAIKYLDCPSADGFLGPFLSTDHAGDDALVSTDDLANVCLLRPDCLGFNSLGYLKAKIVPTAPATDVCLYVRNASFFSSHVCGKMIDSYGVIPGSSWGELSPSPGNRAIYSNASCDVKACTYFQFKYGTDSSTWGSMPSALQFSWNNFYCNQVIQGCPYVDGFLATPNSDHIGHDIANADSIAVAATQCASNPSCQAFNNAGVMKSSAFPINAVTGACLYTKVLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLC7</accession>
    <name>A0ABQ5SLC7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015518</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="58064" checksum="50366D5ED4C63827" modified="2025-10-08" version="1">MANYGVASYAAALGPFFDIHRQLEQQPRPGAMKDQQLQLQHGNLISRPGNLIGRGSPRLASDAVLHPEAAATASVRHSILASSTAAGRLTRSACTLRSRAGHGGNDNGGGVMGKMGAVAALRCHANDWLDVLLQAIDLMDGNSAVTGATAAAVAAAAAAHNVGPHPAVAATAAAAAGGGQAASPGPAVRVTAWVPVKVASSMDSVASEGSWGKSPAVAAALAPGEVDMRLRGRATVGDTFEDVANDDEDIGKQHLVMLRAPDGSSARRNVHLLNLLGLPQEVSSLRLPVSEPEPDRSVVGCNEDHGWSLEILDGAVGEARDPAWALPYGFTYDYDFPPSATMPQALPTAEGSYSLVDSCLGLNTSQSVLALEAVRHHGASQAPPTPQQQQLELQQQQQHRSTPVAVEVLNLLAEPTGAGAAALQEKQQRCRAVPYIGDCLVSYIDCLEWNDGELGGSLQSPGADSASYPGGDMFAFMFSEDELGADVSEVDLEVRCAQVGVMSHTTDGVVATGGAAAAAHGGDCPCNGCRRRRCSPEGGGQWPCRRKEPLLTSLTTGAVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLG2</accession>
    <name>A0ABQ5SLG2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCZ1/INTU/HSP4 first Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="740"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="462"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="547"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="750"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="781"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="799"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="924" mass="93980" checksum="7B899DFC1723B713" modified="2025-10-08" version="1">MTAASHHSVLHAAFIVSTDAGLRARDDDEDHSLVYTYPPSAPAGSTGGFLKSFSSLIVTVRGALDSICGEKTRLIHLEHGGQRLLLASAHLESVMLVLALRSVVPPAAATSLALQLAETLVLLLGPYAEWGLTAGGQSSLSPGPDSGQREETRAILDRIFGGVLKQVDSPSWVSRMAPWTSFEPSCLRLTLPTHMHTRLEALLADHGAQRNRAAFSFFRKHSCLMYRGLLLASDLQPSHARQLWQLLWALGLHQHSGGLPCQVLTHTIHLPQLQLQPPPGTAPATEAAARGASLAAASAAGTAAAAAAAAAVALQAESGVTVSEQRMGRSLLVVAAAFKLVLVVVLTGYDEEIEEELAGRLEGHSAAELLQRVYSCLGADLERLVEGSAIYSGAARALESPSPASSSPPPPPHHLQRPDSVGSGLGSRPGKLQGGMGGSFASGGSLLFRVMTRNRSSPAHRTTNSAGGATWSAGTGAGEPGGGGGGVAGSTASAEATSSPPSGPPVAQSRSPPSLQTHPAQPLRIAISTTLPPGPPITPSTARFPYTANGNNTGGAAPPAGQPHDRGASASGPTLGVRGPGIGDVGANPGGAGGGSGGVQPVMSPFEGVLLMIAHDGPQGCVRFLGDVPRGGGGLGGEVVALAAVWRAVAACRARFGLHNVAVAAPPKHARLTMTLDQILQLSAARSNKLGGQRLGSADSTPWQYHPELASLAAVNTVTLRLQLPPGFAMRTADTQDAVTGDLGGGAAGSGSSSYQQQQRQQGGAAAAVTTTPRAKPSWLNRISVTHRTSNASAHEDSPAGTPNPAAAAGSPEPSTPVSALLPVWSGMDPLSMSQGPMGLGTGPHSSTATTTAHMLAASSSSALYLTGLLLPDTRELYLCHTAEVDEARLAEAIWEVAAGGVAPVIGAGAGPGSLGGKLWGYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLU0</accession>
    <name>A0ABQ5SLU0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_016070</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="565"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="313"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="877"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="313"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="592"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="915" mass="96862" checksum="5DF147E860D0CFFE" modified="2025-10-08" version="1">MRIIRLWSYYDSQWARSWSTDMKCPERVGICTPVCRFRYLTWILICFGLCFTGTGVRAQNSPSCEAENTTAFLSALLSGNCSTVVIAGKGFKMDQAEVARILRDLPEHRVRVDGALTIRGEQPRTLWDLAFLKRRIGIEVNSVVTLEDLFIPNGRQPSATSFGSLDIFAMNSNSGLVMRNVTLVYPLCIPMSIAAFYTPRVQRPSGYPGSQAVRLAGSVCYPDVDFIPTCWGESMLLVSFVSIGSTLDSSGTRLTSGVVQEYNTTYLCENMVSEACIGAKNGTLVCVQQAIQRYGLEHFEQYDLFDHPPLPPFPPAPPPWPPSPPLFPLPPPAPPSPPSPLLPPSPPAPPRPLPLSGVGQQLALMHRGSPLLPVILPSVLGGLLLLAACVGGGALWWWRLRHRRGSGGIEVAMQANGYIKTSNGATHDSRGDHYGLPMGIPPDPQRMLLEVAEPGVPAAGARSTASGIAVPWVSATPGSGAWAPNGEAWVAKADGGAAAAAAVGVGRFTTISSVQSGCTAGGLPSNGGAAVELLLGNPLLGTKGSGCNSGSGIALNMVLGVDVFASREAVVGRGTFATVLEGALGGRPVAVKLFHRDALGGTADDVKSLKQEIAILARCQHPNVVAFLGAGSTVAGDEVFLVEELMVKNLARACHEGSGLPLHRVLSVARDVAAGLAYLHPTVIHRDLKPANVLLDQYGTAKLTDFGLSRLWASTVHTANPEAGTVQYMAPECFNGSLFNDVSGKIPISHQSDIYSFGVLLWEMLARERPWKGLTAIQVALAVGFQQHRLRIPDTDNERFHPKLRKLILRCWDEDPRKRPGAAEIAKRISLLLQHIETHGSLSGPAVFVTVNDSVAGSIGSDTTAGGGAISMTVPVPPVSSGSLGQGPGGTPCPTSEEVDMATAGSGAKPLLCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM52</accession>
    <name>A0ABQ5SM52_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015984</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="198" mass="21713" checksum="0B8BB25CB95E5E20" modified="2025-10-08" version="1">MLHQAILRYKHCDRVLFACTGGVARRCRSVLAARVCQMASMTMNIHGPGADKLRAEGCSSACSRDTPRVEPAELTEYMKALPAWHLNADKTMITRTFTARNFMAAIRFFNKLAEVAEAEGHHPDIHLRNFREVEINLSTHAVGGLTLPDFAMAAKLDALDIEYSPKWAKQEAERLAATVTGQGEDGDGTSVKVEASAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SML2</accession>
    <name>A0ABQ5SML2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="1">
        <location>
            <begin position="34"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="205" mass="23586" checksum="60A515D81593B281" modified="2025-10-08" version="1">MMKIHRVEPVRRLTERLKSRTLQRSTVKVCASTKDGPPTVRVQFSVHYRAHRHQIICIGGSQLPLGWSFLSIAKMPMSWNPGDKWICEVEFPAGQRLEYKYVVLEEQEWTNLADKYAQGYVEVPYRTAADPGRPPDPREIVAMAIVAWQPGPNRVLHVPSEEELKRLGASLTTQQRERNHNTTETLYVSEDGQPTLIRDDVWGWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMP8</accession>
    <name>A0ABQ5SMP8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_016387</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049898">
        <property type="entry name" value="MARR_BRCT_CHROMO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032450">
        <property type="entry name" value="SMARCC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF41">
        <property type="entry name" value="BRAHMA ASSOCIATED PROTEIN 155 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16496">
        <property type="entry name" value="SWIRM-assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52032">
        <property type="entry name" value="MARR_BRCT_CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="9">
        <location>
            <begin position="2"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="7">
        <location>
            <begin position="632"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="894"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="896"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="481"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="586"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1134"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1193"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1298"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="593"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1193"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1298"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1324"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1361" mass="141840" checksum="F90221AEF3769617" modified="2025-10-08" version="1" fragment="single">MDGLIGKRSGKVLPKDYEDAEYLRRLEPVVVQLDEDLPDAGFDAKKLATLVCQLMQFQEDTLGKESNFKRQPKLPASLLRDFSPRGALYIIGAKCDDIMAARDLRRIDWPNPAKRKENMEILIGITKELEAEGLLRLPVVGLESGLGQELLGRLGEAVRRMGGTVVDNPDDPRVTYRICVPPPGVSDAGRVKPQQMRTLEVRGDMALVHWTQLPDSYDEWVLARHAPPERPQQPEEAKHWRVYPRWIKDSEVYNEWMNPADYEADPPAAAPSPPQPPPPVAVAVPAQMTTAAAPLPQQQAQLPLQQQTHQQPQAPQLQPQPQQLTGLLPQQQQQQALQQLTNPATVAAAAPPPLPQLGLGGAAATATATAAAAMTAYQQPGQLAAVVAPAGVAGGGSGPAIGGALGLPDQRPPMKPLQPLQPLQPPGAPGALPIIAPVWRPAAPAVLPVSVGQALSAQLAQPASAAFATATATAAAHATAPPPAVAPAAPAPVPTGPVPTAPLPGSQRGAARAAAAARAAAAAAAAKDDAPAAKRQKAEVYAKPEVVEGVGVRMAVNAVKQRVHHPNKPSVDPTWHVENISQGQTAHFQGAPPSVPRAPTPPGVVCPPPPRPPPPGTAGFAAAATPPKKEPYLVPSSASWFSLNGIHEFERLYLTDFFEGLGPHRNLSVYVQYREAMVSMYREDPSRRLTFSEARSRLQGDVTAIHRVWTFLDYWGIINFSATDHPWERGGGGGGEGPNHLTLALGPPSIYSVRRVTSVPPGAAALFQFSRPTVAEATAAATSGGAAGSAHLSGRGPRTQHLLNRAAGDPPGSRPRFYCSAMPWVDCTDLRYHCIKHPDIDLCPAAFAEGRFPPGITAKDFVRIDATAKHSAGAAAASVGAVGVAVGPPHGAHGAEGAWSQQESLLLLEGLEMFGDNWGEVADHVGTKSQVACILHFLQLPIEDAFLDDMEAGCGPRAGPVPVAAPPGGGGGGGGGGGVRLQQQQATGAAAVAAARQAATNRQAAVAAQTAQTGVTLVNNILDEDLVPFVDHANPVMAQMAFLLSILGPQIAAAAAQRAMEFMSSDDQVAAALREQQQRLQQQQQKEAAAAAAAAAKEAATAQATVQAPTVPQVAGTSDPAAAAQPAAAGVGETAAAATTGRDREGNVAAPAAANAAALPPAAPEASASFATPAPGADGVDGSAAPMAVAGAPASSGAVAGDQPPSAAPPPPPGTDLQPNDNIPPPSMDPSSIPLSAARLRSAAAAGLAAAAARARLLAEEHENDMLEIMSQIVEVQIQKLGLKVKYLEDVEQALESERGQLENSRRNLDEQRSQLRDARRTRAQQQLQLGIPQPQPQQPGLQLQQPQQQPQQQPQLQLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNQ4</accession>
    <name>A0ABQ5SNQ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tim44-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_016871</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim44 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039544">
        <property type="entry name" value="Tim44-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tim44-like" evidence="8">
        <location>
            <begin position="238"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="53"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI71607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="399" mass="44500" checksum="8FEE3B1AF9C56CF6" modified="2025-10-08" version="1">MASQMHHKLLIPLASNVHAQRALFALAQPLAYLGTAHARALSTTRCGLNEQHKPEVTQASTSGSSQQQQQQQYQQETASSSDQSQNLYNSFKAGYEHVKSKTAGVAGGGSGQEPVAKRLLQTLAKDLREVLLPAQDISSATRAYTGPVASTTYDGPTALVLARQQATGWQKTWDTVQEKLSGIPLVSKLLNLRVTDTAAYKKGQEIVEDIKDKYETSDHPVVHKVEDIKARMFTGSEASRAMREIRVRDPSFDMNRFVQSVKLDAPVVVRAFLKHDLEALSQHCGPELLERFTGIFKHFDEQGVFEDPSILFIGDVEIVEVRLMDDDPFIIAQFHCQQLKCTRDKFGNVIDGGANQIQRVYYFWGLQQERSPVVTAEGKVVAPRWVIKDMMWQSMLALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNX1</accession>
    <name>A0ABQ5SNX1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_017017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="119" mass="13050" checksum="39BB220A01C21F4B" modified="2025-10-08" version="1">MATHIFTYSRGLFMMQLSPVGKHARAFGHQRPYFLTSQRKAPCLPWASNSPDPTPSPEPAMPSASEPVASEARNLTTWRYAKLKDPGFGPWFIGMAFLPFVLLLVPLLSSPPMPPAAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNX9</accession>
    <name>A0ABQ5SNX9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_017013</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181:SF3">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="31"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="129"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="277"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI71715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="40365" checksum="3203430860DC42DA" modified="2025-10-08" version="1">MNLQDSKASDGGVVLAAAQQNKRDVERPKKPPSIASALSGAISGSLISACVQPLDVVRTKMQADAARGVVRGTFATARVVLSEQGLRGFWAGTGPSVIRVGLGAGMHFVLLEQIRWLLSYPGPDGSLQLSNVGAAMSGGISRAIAAVLLCPVTVVKTRMEYVADVVKPANSGGTAGPITASISSAGASTGGASTARAAAGAAVSTSGVGPVAAACSSTAVPRYRDTFHALSAIMRAEGVRGLFRGLWPTVLTNAPYSGLYYMFYTRLKEGLSAEGRPQVLVNFSSGVAAAVAATLLTQPADVVRTRMQLGLGAAAAEAASLGAWATLREAVRYQGPAALMTGAAPRILKRTTQTALVWTLYEELVPRISQLGYAAKAAWDQTATTTTSTEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPJ3</accession>
    <name>A0ABQ5SPJ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad21/Rec8-like protein C-terminal eukaryotic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_017197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000125">
        <property type="protein sequence ID" value="GLI71830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="2">
        <location>
            <begin position="117"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="164" mass="17087" checksum="EEDD4B062DE71338" modified="2025-10-08" version="1" fragment="single">GNSKGGGRSEGASGGVSRKSSHQAGSSARGGSGSEKEAADATPFFCLKETNGLATQVLPPQLFMSRNTKVVISIFRKQLAQAQSQAPVQVAEQHQGAGHSNGGDSGISDLQEAALSFFGLASRLKRSEAVKLFYQTLVTHTAGYVVASQYEPYGDITIRAAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLN0</accession>
    <name>A0ABQ5RLN0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chlorophyllase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017395">
        <property type="entry name" value="Chlorophyllase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428:SF14">
        <property type="entry name" value="CARBOXYLESTERASE TYPE B DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428">
        <property type="entry name" value="CHLOROPHYLLASE-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07224">
        <property type="entry name" value="Chlorophyllase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="51650" checksum="2F62E568D66A00FF" modified="2025-10-08" version="1">MTTENDTVTIRLVASGNQSDQREGRSNPPAHQDKAPEGGQSWVAQGNARDKAPHELATPQTPQHPGFLSALYNTLSGVLPCFTCGVFLRHTIHPRDRQPEPPLVATPHSDQELKPMSPPPLPPLPVNCFETTDPPAHVDVSWGPLAGHRLRKKFDLPWGGPKTRVTVTCPEQLSNETFPVAVMFNGFLCKAKWYRGLVDRVVSWGFAVVQYDVEGLIDSAEMAILDPLLQLLSAQVRGGALPAGLDLHQLAVLGHNRGGMLAALHLAKDDLIKAAVLIDPVDMSGESGRWKEVVEELQRALPEGLVRWPDRLTSAAARLPQAVNVTSLQGLQCALQPPAIELPGKPRPRKLVGIVGAAAGTRIPATDQYYKFWSLMPPGSRLLVLPQAGPMQFLDLGAWVPSHLNKILGESDSKDAIEAAAVFTAACLNEFLLGEDSTSPFGWRLGHYQYVVSVKRVEGPSPCIITAAADVVPESSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN45</accession>
    <name>A0ABQ5RN45_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RDRP core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000802</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF55">
        <property type="entry name" value="RNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RDRP core" evidence="2">
        <location>
            <begin position="426"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="RDRP core" evidence="2">
        <location>
            <begin position="851"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="domain" description="RDRP core" evidence="2">
        <location>
            <begin position="1202"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="980"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1083"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1241"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1398"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1083"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1097"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1169"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1414"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1461"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1479"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1512"/>
            <end position="1526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1540" mass="162809" checksum="73CDB1B7D7080C1E" modified="2025-10-08" version="1">MASDHADIAGFPSNHHLLAESLTDWDVLSAVSKSSWEEAIVLLSRRLGCLPDVVHALQLTEKLHLYLHLPLPLRLLLHQHCIALSALPLPRLVDLLNSPPQQLAAALDAVANPYRNPVDCIGNTGWKRRWRPTVEFMSAASAVPVDLNPGEALLLFLSSGWLSYEPGRRGLTGQQPSQPASSGPPVDTLGVPLLPGAELDATAVLEAAAAEPSVNLIALPGQCGGTGVHPGGECRYIWHPEQGNFSPAHECDDPQRAEQHWRLQHQHWLQHRQVSGGLHRTGPLAATEATTRLSSCRTLTAVMEPPPAVGEAPGCAGWPRLARLEYNMYDFPASHLQRHPLIGSSLDRLLFVRFTADMLPQQRQGSYAAPRSRPRPSLHWKYGNSSVAASSTAGKVEVTDPCVDGGWGSTALGARGSADINPLAVLLTDGVVVAGRRYRRLVVKDAGKTGEKICFFAQDTLSFDDVRHGFRSLGPPSAIRQLLADVSMAAEAARRAVAAPTTQVAGVGDAAAAGIRVVAGNEDAMAMTTGNLAASTAAAAKAASRSELFLSGTWPLAIHPASICYTEDVVSSSGAVMTDGNGLICARLARMIPYTYQGAPLVQLPLPYSSSSPARSAANPWDAYPGSVGSSNRQLEVARQVVMPASVHAAAQEASTVTAAGGRKAGPLAMTAAGPVLEPPVQLRMYDSRFSVIVKGTFTPVVSGLPPGVSLLVRASQVKAPSWKKLQMLLTMSRITPGHPASSPGIFVPGYSHPGSLEPAGASTSSTYGEQSSYSRLGSSATDATLLQFLKPAALTIKPGKTVAGYAARNHLGTEKGSQMLLLAPRRQPAGATTAVVKAAAAATAEMGNAVLEVVRKAPARPGAVARLNGPLLLLLSVCCIDARTLMDILKEELGKVLAAQRGDLKAARELATRGGRCNSDDDALLDGLRSAGNDADASTAADASAAARTTTTLQPHDLPCSTAGVSAESGGGGLWCAQSRQRNVSETHHHHHQQQQQGQGQQRRAREEELRGGGGTELQRLGLDYRTRELLQACATRAWRQLWELRLPLLRSASIMGLPDPSASIPEGCVVLLPELESESGLGQRLGSSVDGSLDSSRREGGTRVERQLEREREVVVYRFPGLHPRDLRKFRVIAPPPQLLSQLGLTPTGTSSSPSDSPESCSQTAFEGGGGAGGGNRSGRRFGGGNILERGGGGEGGRLLFFSTSGSQAPVYGMSGGDYDGDQYTVIWDERVVERFEAYPDEAESGTGGRDGQRLAASSRNARCKGESDCSSADDEARWEPFQAQRVLLEPPISARVAKTQATDTAIGAVTGVRDCEVEDAAAEEEEEEYLTRRYLFCHEAANVVTEAYRLWIWCADEYGADHPYSLMLCRLYSEALDAPKTGAFRRIPFNLRDKVRPPHWAPGPGAPSPSESHIASGSTLPASGAAIQPREESAIQVKKGAASSTDAGLNASGPRNSAAATAVAVTVPVTGRDRRRRQELIRTDAQRPSGPCDPWGSDGPSGALTWTTSSADDSVDVGSSGADEGAGGEAGPFVSPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPF8</accession>
    <name>A0ABQ5RPF8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001317</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23121">
        <property type="entry name" value="SODIUM-DEPENDENT GLUCOSE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23121:SF9">
        <property type="entry name" value="SODIUM-DEPENDENT GLUCOSE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="512"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="545"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="574"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="41"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="200"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="614"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="623"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="637"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="668" mass="66282" checksum="34BA04F90CF16B35" modified="2025-10-08" version="1" fragment="single">ADGHGGGGGTPFSLHAGSAAAAYYAVAAGAATCAIWAASLPSPQRPEPHHHHHHQQQQQQNEDVAVDGGRQSAPTQRVKPKRAAAGDNSGGGGGGSPDEVLSSPSFATVLDLSGLEPPTGTYADAYADALESLAAAAEGSAAWGTSSTTSPDGVTDSPIVSLPAAVLPAASGAGLATQARLMGTKSRTSLSATLVCDGDCSSSSSSGGIGGGGGGGSVSGTDGGFGSALSTSPTAWDETGSGSMDQSYIQGGEEPGFSAATTADVATAAPAAFSSNGCGCTKAAAPRNTASDILSAAAAQIKLLQGQCEQHQRYGAEGASMTSPGGWWRRDMAAAEGGEGAGEVGVSGERLASAASATPPPPLSPTAALFRRYLHELRMGWPVLLPVVLLLFANISTQASIGAWVTTYCQRAAGLDEATSHAVTAAYWAAFTGTRMAAVAAAPFLHASRVLLVTTPFAVAGAGLAVAGLPKISWLFPHGPATAEATAAAVAAAAAAGGGGGGGALAVYLPLWALYTAVCLVGVGISTGFANTISLTGDHLQLDGFTNGILSSVAGLASTLCPAAVPWLAAHTQLGYGALMAVALAMSGVQLAMVVGALIGARWVDEWQRLEAEEEERGEGQARWRRKAVRKKRGGAEDSSSSGEAGVMVVLVVGEGQEGKMQGGPPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPK4</accession>
    <name>A0ABQ5RPK4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Threonylcarbamoyladenosine tRNA methylthiotransferase</fullName>
            <ecNumber evidence="4">2.8.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">tRNA-t(6)A37 methylthiotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">VaNZ11_001370</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="915"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="18">
        <location>
            <begin position="63"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="19">
        <location>
            <begin position="194"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="17">
        <location>
            <begin position="430"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="507"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="744"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="530"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="789"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="GLI59485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="944" mass="98561" checksum="B07D1C633B167482" modified="2025-10-08" version="1">MTIACADEVGDIEDFDGAGADDGRPMKPAFLGRHVRKALPPGNDVCARAARPHSSILEVPGTQSVWVKTFGCSHNISDSEYMVGQLVDYGYRLVDDAARDTADLWLINSCTVKGPSQAGMSTLIAAGRANGKRLLVAGCVPQGDKRLPELQGVSVLGVTQIDRVVEAVEETLRGNTVSLLAKKELPRLDLPKVRRNRHIEIVPISTGCLGSCTYCKTKHARGHLGSYDPEALAERVRQAAADPWVREIWLSSEDTGAYGRDICSSLPELLDALIAVLPPDGRTMLRVGMTNPPYVLEHLRALSSALRHPCVFSYLHVPVQSGSDAVLEAMKREYTVVEFRRVVDTLLAEVPGMELATDIITAFPGETLDDHAATLELLREYRFPHTHISQFYPRPGTPAARMRPRVPGAEAKRRSRELAAEVDGWGTVYEHLVGTRQRVVVVDTAADGHHLVGHTGSYAQVLLEPQPGLMGAVVEVQITSSSRWSVRGVVVSWLYRPENVMADVQEGLSRPPQQGSRQRAGPEASPHRPRAPDPDDSTNRHVCPDDPISGSHQHGNREDLPLQVPRESDAQTSGHDNGISGGYASCKPENHLASASASPFASASPFAYADGVISTSGRQLLASSARSPPAVETCCGGVCESGACVSGSISGAAIETASDVHMVEIHGATSSTSPAAPTAIAMKSGSSRFCHQNSCNENSGNCDGARVDVDASSGCGSGDSSTGCCGGSSWDACGLGAATASPYGQVSNNPGGSSHEERQHVTAGQTAEAVASMQHPAPLAMPQTVMGCDQGSLSSSDGVGSDPPKAKMEGASAPVVVLSSQVAKTLSATTTTAAGMTVTAAAIAATKVATPPSGLPAEAFRPTLRKAGASAAPAGKIEAAPAGLGIPAVVVAASRSAVGAVTAAVPLHVPLIDRVLYLALAVGLVGILCSGTLTIWNTIHGTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPL7</accession>
    <name>A0ABQ5RPL7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_000874</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF140">
        <property type="entry name" value="INACTIVE PROTEIN TYROSINE KINASE PTKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="152"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="318"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="483"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="335"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="483"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI59046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="516" mass="57368" checksum="67EA76475E639C96" modified="2025-10-08" version="1">MSQPQDQDSLHGENAARRQAITELLFFASVGDLYRCKKIIYAWGLNIKDASCCDYDRRTPLHLAAAEGAFSVVLWLLDHGAEVNPIDRFKRTPLEDAVRGDHGDLATLLIQRGGRVLDKEGNLVELADSPLAGNVRIFTDYDPEWEIDPSNIKLSEKIGEGEFGVVYKANWNGTLVAVKVLKETGAVALGDFRTELNVLQKVHHPHTVQFLGAVTKQTPFMIVTEYMVGGSLADLFKGQRFPTMWRSVQLALDMARGLAYLHNRSPQAVIHRDLKPANLMIGGPKVFSAYHRQICLEEMGVLKIADFGLSKSLKLTRPKRHRGSLDMTPDNSVLNGRPGPNGPNSGLGTPKGSVHEGDKTGAATTSYKLTGETGSYRYMAPEVFRHEPYNNKVDVYSFAMICFQLFEGLPPYWNMDPIEAARAAALKGLRPQWGPTNRRDQVVPPRLKRLVETCWSADYETRPEFVEVIEMLEEVSKELKPDLMENRRPASAVKREPQAQPNAAGSSSSSCCCTLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPQ7</accession>
    <name>A0ABQ5RPQ7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001449</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="228" mass="25297" checksum="C1A17AE7CE931333" modified="2025-10-08" version="1">MSSYRIKLVLSDAVRKYLYEEKLNLVVGKAVGTVERPTYRTAWHVLTPSELAQTVELTWKHNYTGSFTTDKIYDRFSKYTTQGNDIQMAAGHIYDVTAYGTMIENPNKPPRPNSNRFFFCNGMGYRRVFHPVLKTPAPGASASSPGAPFWLGDHVQKNGAVIATPVEKVIVWFGKYNAGAAILEEVMTVAVEFDLTEITSGSAEIADDLSAWKNLSPNAQEVDVVPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPR6</accession>
    <name>A0ABQ5RPR6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36343">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36343:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="111" mass="11898" checksum="03CA15102EB0AB20" modified="2025-10-08" version="1">MASIALKPASAIVARRNVSSEAICRPASSRRFLCTKALLGPDGKPGAAGAKKKFITREEEPEQYWSSKGELDGANPLKDPLAIIGILAIFFPFIFLAVAIGTGYVDISVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ62</accession>
    <name>A0ABQ5RQ62_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="11281" checksum="59AADE03B8C4D185" modified="2025-10-08" version="1" fragment="single">STPLAPFVATSVQQYSSSSNGDEVLVLSMHPDPRTASATGSSPSIIPADDEARLFFTVAYNSDDAVTPGVHPALIYRRIAFGSGVDPKLYNSLETGDALQIAGQQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ78</accession>
    <name>A0ABQ5RQ78_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="376" mass="38752" checksum="F2321D3F53BAB3BE" modified="2025-10-08" version="1">MMLASEPIMRRLPASTGSRSCSTRFNSTQILSRTSRRSCKPVCFKENTPDRIDDATIGRPSDAMPNVAATPPRSYGLESGMSTAGLRSVNLGPVASVMYPILTYGTLASAAAAILAPNAVGIFLFRNAALTAVHKMMIMCAGAALIPSVFAKRQIKRAADSGILASPTYKQMVSACLLNGVLSLVVLSQAVSLRNPLLAASVGGITALATLTASYTLMKVRESGQLAPSAGGLLGGLMSLITPSNVTAAAYSVSTIALSVVGVMMFTADPATPSMLFILPKDAVHVFCSRIIGGATLANAVLLFTVKQAADDGRQGSPAFRNINAGIASYAAITAGVLGYGLATGWAKSTPPVWGLCGVMVTTALLSGFNWASAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ87</accession>
    <name>A0ABQ5RQ87_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione-specific gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="1">4.3.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.7" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006840">
        <property type="entry name" value="ChaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192">
        <property type="entry name" value="CATION TRANSPORT PROTEIN CHAC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192:SF2">
        <property type="entry name" value="GLUTATHIONE-SPECIFIC GAMMA-GLUTAMYLCYCLOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04752">
        <property type="entry name" value="ChaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="271" mass="30224" checksum="4C3FC66006AEAA30" modified="2025-10-08" version="1" fragment="single">DRRYELIVIIFYLVSNNLNTSDGVTWLKKCQHYSCYLELALFMSTVAECDKNATSTWVFGYGSLIHTPNFLYDQRVVGHIRGYRRVFWQGSTDHRGTPERPGRTVTLTAENHPAAVTWGVAFQLAGTSQEQAATLSYLEWREKQYDVRQLVDVYDAHGGILVRGALVYIASPANRNYLGPADTQIIAAQIATSRGPSGPNWEYLFKLADAMRSIGVPDGELFALEDMVREEILRQQELRSMNGLPAAVAEGPPNMFALDPLAGVAMKFTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQF7</accession>
    <name>A0ABQ5RQF7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="121" mass="7048" checksum="FD635CA005EEA04D" modified="2025-10-08" version="1" fragment="single">GGGGGGGGGGGGGGGVGGGGGGGVGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGVGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQQ4</accession>
    <name>A0ABQ5RQQ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_001211</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="283" mass="30413" checksum="ECEA119EFB2A701F" modified="2025-10-08" version="1" fragment="single">LRSILSCTCRQRKMILSRSVVNVQAKLTKKGSAGPKKAAPPPGPRKTLRERAGWWGNGNSDRLSQFYGPDRGLWLGPLSGSAPSYLTGEFPGDYGWDSAGLSSDPETFKRYRELELIHARWAMLGALGCVTPELLAKNGTPIAEPVWFKAGAQIFQEGGLDYLGNPGLVHAQSILATLAAQVILMGAIEGYRVNGGPAGEGLDKLHPGGPWFDPLSLAEDPDAFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPIENLESHLADPSVNNAFAFATKFTPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQX7</accession>
    <name>A0ABQ5RQX7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001653</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01228">
        <property type="entry name" value="EGGSHELL"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="569"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="596" mass="58040" checksum="017D9381030C275C" modified="2025-10-08" version="1">MLGAGANWADEVEEEESQRSAAVFPRVVSPTKPSVARDTSPAVTTVTDEGDWRAAKDARASAVGGHAIRATANSSVTKNHIASSNHVVGQAVNSLATDSEPKTAAPKPASPARPSRTTPDMTTVSLTLGADSQLPSSVGVRRLTGAGANSHDSDAATTPAVAASATGDVGVAAADAAMAATAIPPPSNGTVAAMAATLRSVAEDVARQYCDAVVAAAAAWLANLSGGLAAAVAAAAEDHIRNLSPSIAAELPHDWSEQLGESLRRGLTSALGPSPGGQVLQDAQQQLHSAVQTAMQGLAAAAPPPPLRQKQQSGGGGSGGGGSRRGVRGGGGRDRERGRERDHERKDALEDEAGSTGGGGGSVGGGGGGGGGGGGRRGSSRREGPQASAASDRSPSAATSGGGGGGGEGRRGGGGGAGGRDRGTGGSGRSRGGGGGGSGGPDVDAGGPPHPRFGGRGFQHDDRDLVDLGEGSPGGAGGGGGSNGGWWRRHDDEVDGGPPGSTQSKRRSEERTGSQPGGTPSAVAAMQPPRKVKAGHQAYTPPARRGTDRQTDGDGDWACGGGAGGAGEESGSSLPPGLNIGGSGGGRRGTRDGVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRU1</accession>
    <name>A0ABQ5RRU1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="524"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="753"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="853"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="960"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="925"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="962"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1002" mass="100963" checksum="907EDE0FA8CF9FE6" modified="2025-10-08" version="1">MFALSPSAATVTTCMPASKRPTWVLLHRRRCRPSLRPLTDGYRSVTVALAAPTGRPTPTPTSRPPEAALLPAAPWELPAPNYDTEAAADSTVATVTASAALVGTAVAPCGSVLQAAAFDCTSIVTAAGPGGMGATASTSEDGSSSGSSSSAVLSSNGSNAVGFRSIASSGRDVEGAGLMGRGNSATADADAKSVSSSNGVVSIVRSDAWLPKAGQGWGNGARAGSGSGPKYVSRGGRGGRSFKQGRGWRLAADTASAHDEGGGGGSGSGSSSGLRLDSGRGGGRGSTVPPSSLTAVNSPLLQELQAMQQQVAELRTLISEQQRREVEQRQQMEALLQYVLSGGKVPIPPEIKALPAAATANTTTAATVTAATVAAVASADHAPSASVASELQKLQRIENAADLASNGPEGTHSTAIAPPPSPPLHPLKNPDAESASIPVAAADMPGVPNVAPTELTINNTVVGGSIRRGSDSSSNNDSDVSVKVDDKEEEEAQVSPVSLAVQPELLTLLEQELNAGISSSNGNAVLGGSSNGSTDAKPTGSDSTVNGRAASGHYRRTDSVVSTLSENRTIISNTAAVEKMSVDVAVGIQNEKQGGNITGAAPPSLTSSVNGADVEVTHGSANNLQEELRHLFALELQHAAAPSAPGDVITSSQGAVETVGMGDRTSTVAPSPMISLLLLAASEKADMTADVPLPPLQLSSSCLEVATAAAHPQTATVSITAAIPPFHPAAASVSTSSGSSSSQMMNITHDIITTSTSSDSNDSSDSGAGSRSAALANESELRDIVAQLMSVASETARLARQAKHAGVPLPPELQQPAALPIALALLQRLKASKAGAGGEPLGRAPAAAVAPSAVLPESSAARKPPRRSGSGYPSKEWQQRSEFRSGGGAVGGGADGRLSSNGSSGGGSRSDRTEAGAWSAAPVASPTQEVTELTSDQLAALQTHPSYMFQGLRRLSWEGEEPLAAPAPSASSSRSSESAGLETPPPPLLRPPSRSARTEMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSK2</accession>
    <name>A0ABQ5RSK2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="781"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="801" mass="87753" checksum="F4AC98DEA5B5B664" modified="2025-10-08" version="1">MRAHIPFVIVTILVAYGSISLGITNDEVIGLRPNKIAAGAASRRVIAQLPESDEVASILQGAILKSFVGWSIMGDTAPLKDQDGAEYDEEASEQKLLSQMKQLLLMVGNLLRPDKGCRGKMGSDMRILSRSAACPAAAPELRQGSTSSFLDSILQTYPGGPTDREALIAWAAAATAFLEEHRRLQRQHRKIAQRQGMDPAAAALAQQNAAPVGTAAEGTAASSPAPAKANPVAMTATGPSTAEAFHTKSSAHNPPAGASAKPTSMLAGSLEETADMPRPSLSGDHQQGMQQHRQQRRRQRQRCRRHLLEASSGDGTAWPGEGRGDDDSRLSRIVMFRGRDLTTAARGRHAHDAPFSTAGKRRRLKEPGVTDGSAQNVTHHMSATATASTVGGLPLVRRVLLAQLLLGWATELRRELPPTPEVATAVAQVLAKLYYIHVDSVVDRAALEYFHISKAGGTSWTAAAKANGCSVPRARAARTPEFDDDCRWFDPEVLVQMGHTEVQAARTFVCPRYGIIERRSPVRDCSRRLDHFRRTGLQYTANEYTLHGGRDDMYGTHICPQFVTVATIREPLGRLVSNIRYMMVQMKRDKAMSVETFSKHFCNRSAADWEVYSPPVADNYNIRTLVGERAFHSRLYEIGEAHETVAKQLLMQFDLLLDLNAGDKAALLLMRAGLGWNTTLANVHIWSSEKLAKKGSLDFNTCRMGDLELLLARQVYDQRWYSFGHALSHLDQLFLSTAVQLGLQPWPEDWQERQLGPIPEDGVVHSKCGLVGLVPPQQHPHRPAPAIANLHQRSPPPGQPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSN3</accession>
    <name>A0ABQ5RSN3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="155" mass="17698" checksum="9B0B1105FBC76D16" modified="2025-10-08" version="1" fragment="single">PIIDIRKRELNSSIPASVIVIANFLQRCSLIQVTWLVQEHYMFCLPWVLSARTLGSALSQGGFAVAGKPSWSTRSYATQGEETQRAFRRPMRYGSKLSVRDPGRFQRMKAILGYPDDTSPGKVARVMISMLQTMKYLMSGVKNWFYFRAWLAIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSP0</accession>
    <name>A0ABQ5RSP0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="101" mass="10337" checksum="781F0C2D3D5DCCFB" modified="2025-10-08" version="1">MSILTLNRAASYSPDCSSPSAAAAIAATWPAAVAKVPDTGGGGGNSCPTCEPLFPLDAAMPASFPCAAGDLRQYSYTLRNLHANVLYAAARGDLNVLLYMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTQ4</accession>
    <name>A0ABQ5RTQ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_002647</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="8">
        <location>
            <begin position="14"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="308"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="371" mass="39991" checksum="9D8F63852163ED27" modified="2025-10-08" version="1">MAIQKRDPGNPVVYLDITIGGESVGRALLELYKDVVPKTAENFRALCTGEKGVGEKGVPLCFKGSTFHRVIPDFMIQGGDFTAGNGTGGESIYGDRFEDENFELLHETPGLLSMANAGPNTNGSQFFITTVPTPHLNGKHVVFGRVLQGMGVVREIEQTETGEQDRPVQPVVISDCGEISKEQLEAAATAAAGTSSFPAFPEDAQKPEGETEAEFRLRAGEQIRVEGNELFKDGKYGEAVQRYSSALRYVGQDNFSDPDAATEEQKTALQQAVISCLLNRAACRVRLGKPEAALADAEAVLDQAPDNVKALFRAGQAKISLKNYKDALVDLKRASELAPSDTGIAGELAKCKAALDAERKRERATYARMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTW4</accession>
    <name>A0ABQ5RTW4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Diphthine--ammonia ligase</fullName>
            <ecNumber evidence="1">6.3.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Diphthamide synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Diphthamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_003310</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphthine-[translation elongation factor 2] + NH4(+) + ATP = diphthamide-[translation elongation factor 2] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10174"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16692"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.14"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.14" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="622"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="793"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="856"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1007"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1099"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1151"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1313"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1514"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1587"/>
            <end position="1625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1637"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="648"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="800"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="880"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1007"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1020"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1099"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1151"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1326"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1370"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1522"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1608"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1645"/>
            <end position="1671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1736" mass="176556" checksum="8A93FC0AE4227956" modified="2025-10-08" version="1">MRVVALVSGGKDSCYSMQLCKQHGHDIVALANLLPEESASDELDSFCFQTVGHQLIGAYASCMGLPLFRKRISGTSSVQDLGYSPTEGDEVEDLYHLLAYVKENVPGIQAVASGAIASDYQRLRVENVCSRLNLVSLAYLWHQPQRLLLRGMVESGVEAILVKVACLGLHPRMHLGRTITAMEPTLLSLADAYGCNVCGEGGEYETLTLDCPVFTRGRIELDESEIVHGTSDVAHLVPKAFRVVAKPGWEGCELPSDVRLVPGDYVAAPRASRCSASQLPSLAAASATATAPGGGGAAGGNSIAAAVGSGGSTAAAACGVDSLLQPVAPSSFHVHVSMCSGPDYVQLLCRAVRPRSVLPSDLTECDTTAALHALLATAEQELASQNMDLHHCLFVHLFLANMGHFQAANGVYCKHFPSRDPPSRACVQVCLPHDCPVMCEFLLARGAAPPAVAPRSSLSAACPPPPPSPVTRHARRVLHVQSISSWAPSCIGPYSQATSYRGLVYLAGVIPLDPPVMTLVPGDITAQVLRAMMSCEAVAVAQRTTVVGAALGLTVYLAAAVGGGGAVSSNLTPGSLGGGGGGTALERSGMSFSSSGGAELRALTRSAVEAILEHGLPGYAHSPYRQNNAGPPSAGSGGCSSSGRYHRSSTCESGIATPRSSHGTWPGAAGQGRVSAPPSRRSMLLGADGLDEEEEEAERVAAAAVTSGGPAAGFFGGRVTSGGQLFAAAPVLDPYLQPPEVRVSIRPSVLYVTVPSLPRGSLVEIQPVCLDVEALSVIRKLTGHDSFVKRRNNSQHDALHQSAHNQQHGNNNNQNNHHSTAAAGVPLAQPSAANAHGLVTAVGVIHSSPTAGGGTAIGSITSWSELPPTPRASLESLTGRDGGGGGGGGGGSSHNHSLHHPPSSGSSPMHWQVSADLLAMGQLASPLPSHMLSATGFLSPEAGCSLAGTPSGVPFALSRQRSSAVGLLAMSGSGNVGAGACGGYMPAGFSLRAGSSSNLYQRCLSPSPQPLPTASSPQVPGMQAGLLPPAPPAAAAAGGPLQLPMLQTPPHPPLSTQNSSHQLLSPTLQHLLQMQPSPPLLLQPLQQATMDAPPLQQFQQLQIQQQQRQSLARPTPMGAVSRRRRVSSSNGGAAASRDALEAAATAGMAGRASYSGSSSSNSGPLGHHRSSLDGNILSFEVSSGRGGPPVVVSPLPHSVPHSVNAASLVDVPATLPATVAGAVASDGNGNATTVRARPAAITVPQSSVGGSLIQRSPFETAVGTAAGAVAAAAGGMALSAPGITTDVAAAMLAAGGASPRLCAATASGMAGPCPPPSHFPPALTIPPQSSGGSGSDSTPTPGQHATVAGRGTPPVSEMPTAPSGAGTRLGPAPASGSSSNKNSSSDVMVTTVCCYGQVLRSLFHLPPAVFAPGVTTAQLTRRFRRAAASVLAAHGMDMSCLIWVRLWYQKGSVEFMDDEYGTVNSSMPHASTLATVAAGSGAHHGAVAAAAAAAAIPVGGISSGFGPRVLHALENDADDDTGGGGSGGSGGGGSSWHRGKSLRSASSNSDSNSGGGGATNQNGATAHGLGCSTVSCASQRPSASPRLASAAEAVEVPNGPASSPRCHSGSPAAGVVSSSAHNRRVSSVQPDILALDDRQSQQSHQHQHHHQHHQHSQHHHHTHGLSHHQQHHQQQGPKDGPTNELGQGQGQPAPLPPLQQHFHVSWVPVQRVATNASPQLAQCVAVLELLAALPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU18</accession>
    <name>A0ABQ5RU18_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="356" mass="37973" checksum="1069D3ED46117331" modified="2025-10-08" version="1">MSLVLARNEADASGRNYLIIKDVANQLRNLGAPITDRLLNSWVDARKSLKRIIVKDPILLGRLKDLQVVGRRASAIKLASCAAICRAIVNVAILQPLCLAIEELKKAHPPLHATCAQVTPVQLKRAEVYEARLPAAKRLCTISSAAAEGARDEAEAEKDNQPVPNKTSQQEDHCYSQNPITKITNSSREHGTCDEPADAPNNIPIIKKAQQVIATDEQRQQQQQQPPTAAAAFAPSAANAPAAATPADAAPSISTPHQEPPVCKLAASDSICTKPVSPTPIPRTFPAAPAGHRFSRRPLISRCIPPVMSTKTFHQQAGPIALAGGPKTSPPPPRLLPPPPAAAASRYYPPLTLLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUB7</accession>
    <name>A0ABQ5RUB7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="24779" checksum="387FBDBED544990A" modified="2025-10-08" version="1">MAPRKHEAVRKTFSALELISGNSIEDDSPARSGRVEFEASGIRTPGPAGAAMASSPDATESALVTASSLPLSGVTAPSAAPSGLENLVKGLSSPSFYTSLASPHKVGVNVHGAAAGTSAVKTAAATAAFALSMTFSATEGVLGSACSCHDAQIVHTDSDLMAYGCNAGRAACSLTDLAAAAEAAWARCPCWQPSGYAMCGLADHAMTAKPAMSLSTKRGLYEGAVRGGNVRNTWLYITTSRNPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUH7</accession>
    <name>A0ABQ5RUH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003598</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF123">
        <property type="entry name" value="PROTEIN HESO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Right handed beta helix" evidence="2">
        <location>
            <begin position="1665"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="710"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="841"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="965"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1070"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1223"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1309"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1633"/>
            <end position="1666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1886"/>
            <end position="1946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="583"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="717"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="742"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="841"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="852"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1070"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1223"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1278"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1327"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1915"/>
            <end position="1924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2002" mass="202560" checksum="E3AB5A7FAA7383FE" modified="2025-10-08" version="1">MSSMENLIGQAWDEYTKQRQGPSSSLVGTLEDLWHKIAQMEAPTPEDEEARLEICADLTERLASLGFQLGGFLAAGFHGSFLMGCFNNSSALEVAVTGKLPGSAGPNTLVDVELRSSAERLALLERAHSLIAASGIAAQGTVNINRTARVPSVSFEHAGSGIPVRVCIAYPNFALRPQVVRGLVQLEPRLRGLIQLVELWAEAHGLNNPAAGTFNSWALVNLVIFSAQTFQPQPLLPPLWRVCGALDPSAAAGTGPGGANSTTSSGGSIAASTGSFSGSGGILALGNLGGGSTSTAGAMPQTVRPTQRLPGLTASSEGLPSVLREIQSRLTGPPEVLGWPAGGHADPPAPGGLLSLFHWFLVVLDRLLAHWQLSWNRRWRVSTWSGQLVQQPFNRAFLVPVECPFDPADNAAKSLGCENQLKHQQVLRRVVEEVSASLRLLEGTKTPEDLDRLILGMFGLEVEHLYLEEEEECPREVVGIEEGAEEREVQGKQEPMVPPGPLSSRNLDDEPSRRQEDGAAASGRGRLDTSPSFYGDAGDKPGTKKASLPATSNDAPPASTGSAISSPGLLVRLLQGEQQHAVSPASRASPRATETRSGGNGKGGNSNGKAIAANRGAMLPGADSAGAAARTLVSTNDPSSGDLLMDVKVARVEVDDVDDTVVTTAGGSGMCGQQSAAAGESPALPTSSAAAITAASPVVIRGNARVQQPLSPLLRDVTNSRQYGHRNSQSGPEPKGGISVNPSAPSTSTATPSTESPPEESLLPFEGLFGSLLQGARRLFDNMAQGMAELSEDLNELAATTAATIRAVINDDEDLPFLGSSSAGLRRQRRQRLQLLHRGVHGGAANSGGAGSSPASSTGTSQPPSQGKQRPLELSALGSSAGRPGLTRASPLQSEDRCGAGSATPGRRAGPVSTASPGRRSPHVPRTVTPSATASPAGGNLVRSAAKRRSEGLTASLMAAATVATSAVAPSPPRLHPKSVSEAATGGDSHPGKLELARELNGSDAPKSGDLGPYGCSVAARSAAKAGSLSLQATDPASLLGGSLEVPNTAASTEHQAMLLGTPVGRCGRRPLAPSSRPAAPIPISGGPTPEQVSTDKAADQLLAAAAAAPNLELSTDGMANAEPQAISSEGLGGICAVGAADEPSDALLLRGVADARLPLAKTQDAALEKPDEESTAQQATTAVVPLTLGFLHLDSHDSGCLADGGPTSTCGASLTLASSPTADASLSTASSPTIASRRSFAPTPPRKSGPGHSQPLARADLVPATSSGVKAGATHNAPPPPPLLPPSPRPPVGIGGLRVKSAAFRKGTARDTFASPSATGRGFPGAPITSANTNTTATASPGPGPGPGGSGSGSSSAPFFLSASATITANTALDSSLGLTTARTFLQLSSSRLQELAECIDRAGPGTTIDLQGNTFTGPLAAPVMKVTAPGLWLVNGELVLNPEQQLVVVAKAARLDDIIIRDAAPAVAAAPAGPIPAPAASAGGAIRPALLVTKQSYVGGGELTLTRCRLLLGASRSVGLQVKGKGCSAVLEDCVVGGSSVAAAQASDGVMSLSRCTLEAGGGHGVCCRDNASVTVTDCRVRRFRGACAAVMVGGSLELVSCRFNGPYISNQDKEAGDDTLGAADGAGDVAAVTQPEEVTSSDTDSDGGGRDEGGGDTATTSACGPGVLAEGKGSLVMARGSLFEGFRSQAALVVTAGASASLEDCRATGCQQGAVVGTRGGRLLAARCELSSHEAHGLAIGEGATADLRDCRLQGNGASGLHVGGVGSMAVLRGCELLANTGSGVEVAAGGCANLDSCTARANGSAVGVRSGGCGGFDRGGQQGYMGRYGDGLYVEGTGARVTALRCTFSGNMGAGVRVGADGSVQLQDCGIYRNNDSSRAAYGRCSSQTGSGEGSSGGCDAVQPGRAGSIQAGTMNSEGSTDADPRDTDGLGTQFAEESGRGEMRVGVEVEVEAYGRLSLSKGCIWRSPSGDGAAGGEGPEHAVLVAELGCLERSEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUK0</accession>
    <name>A0ABQ5RUK0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PKD/REJ-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003092</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36721:SF1">
        <property type="entry name" value="OS04G0446401 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36721">
        <property type="entry name" value="PROLINE-RICH FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047284895" description="PKD/REJ-like domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1533"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1478"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1612"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1489"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1614"/>
            <end position="1626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1652" mass="170921" checksum="F5266CA831EB57B0" modified="2025-10-08" version="1">MRVHIFCKALVLAVCCLAATQGRPGDAQQSFAIHRVLTASSPTYGNPPPARYPVVPTASPPVAPSPPSGHPLSPPPRVPSYPPVMLAPPPSSPLPSTPLPSPSLPSPSLPSPSLPSPSLPSPSLPSPSLPSPPPPRPTAPRFPPSSPCSDCQFCLDKLHGVTRKAFANLNPSNMSDVTALLFKDECESLPAQYTAGCARVAANIAQSVSNFLAARAGVLCTQLGACKASSACNNLRGTLPDGTAVSGKLDQCTQEGVAGGSVVTLPTVARWNKACRGFWDCQAKGDVCLMGLNGTPQAECFCEYGRDTCYYLGTCTPFCDLASTAVQLQRLNAGAQDCTPGNDTCGAGNLCVSQPGCRVWSCDTTTNSLVSKSCGGRCVPQQLNVISAKLVDSGAGVNLRLNAYATKLTNTPCEYIFDNATVQLLGGPGNAQCNTTNNILYVRLPANATIQPNQKMVLRSSGSLLTSQASPSATFSGNVTLQTCSNCATPNPVILGPSAMEAPCAGDNLLNAAAAVPPSFDASRSSHPSGRLAWKNVVWSVTAGPTAGQTLLSAIISRTNNITNPNDRLQLKLTLNESIELAEGQGYTIRVTLTSWLGTTNSTSFVFKKEGSSSPPAVKVVGPRVQQFLLSSGLRVGAEAGQVCAGKAVNWLWTSTWAGFPGNGVATQQLYLAPPLLAKHGTEIKLRISASYSGSNISSYDEVTMVTLGSKPIARVRGPSGKVPSNGTLIFNATSSRDPDITPDLQQLQYSWECRREDYPNPCFNGAEQGDQSTDGVWQIPASLLTTEIRHTIIVTVNKVVPIGTSPLASTASTEFELSAPGSFPTGNLTRQCSPDQCAKPHNTADDLVVLLTLDTDFITGTTVVWESKDVPGISTLTAVRTVTGAIMLSLPSAILPTNLDEISITAKLTWKGVGGLAFLKVSLDAPPFCTLGEPSQCLQVILDNDQYPQAVATISAIGWRDYLDGTDLTYEFGTVNGSNVNLQQSGSTTTAVVLGLFYGQVTLYGCAVDSGGGRTCASVTITVRTPPATFDARLTLSSINISSVTSSRSRTKLFGAANQCGCLFRWFQIFSSFGHHRRLGRMLQTTEANDAVSTQTIGLINAIMEGTNLEDLEQVKLTLSSVTNTANNAANLLTAEAAKTVLAALDKLITALSSSSNAAAVSTTAATQVCSVLAAAVSPTLIQDSSISWLKNVASSTSKAAAGLGKQAVPGSFLAAGTDGIYISAAALALTGDSFAYTTVRSGPTAGATAVATRRSTLELLVPQVELQPYPRFQLENSVRKLQEAGASSSNQIFVAGVGGKDLDGTSAAGESSDALPYSRRSLITTSTNAEAVLTFSGSSAVDASTFSLGLTHVTQVKDTTAVADVLVSALGSSLPSDVVLASGMVDVRWSYVTSSSLPEPTLDGTNSYITLTIPATSSYNDAKVKACLQYDSSSNTIQGSLSSLTAGTATATFVGYDGNTGLVTCRITAPGTYIVGLAPPRSPPPAQSSSQPPAGASSPPSTGASSPPPVMKEEKTEVVNSPPPEKDSSSIVIGGIVGGVGGAMLIAVIAALVILQRRQRQSIRASNTVSVEVGPGGSRAGGSNATWAFAQPPATDGSGPVTPVATGGNVDTGEQPQGNYNLSPSAGDFVLPNTPKNAPGRMALPALAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUK2</accession>
    <name>A0ABQ5RUK2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="138" mass="15217" checksum="2F3BAAEEDB906CA2" modified="2025-10-08" version="1" fragment="single">STFGIIMAMLARTADICACARDSPRRAALLVTAASGKNGPSSKGLKAGPTLDYGNDWYKKTRELAKPRTVREEIEYRRQANLIANNGKERKDLYTDNWDGSEYKGSPFNILTLVGALFILTPVAGLVFAYYTYGTLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUW3</accession>
    <name>A0ABQ5RUW3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acetyl-CoA C-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003338</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF156">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="7">
        <location>
            <begin position="15"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase N-terminal" evidence="7">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="8">
        <location>
            <begin position="338"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="49046" checksum="A7CB3EBFCD27114C" modified="2025-10-08" version="1">MSGSTVGLSPRTPCIVSAVRTPLGAFQGALSSYTATELGAFAIRGALERAGVRLEDVDEVIFGNVISANLGQAPATQAALGAGLPPSTPCTLVNKVCSSGLKAVMLAAQAIMAGSADVIVSGGMESMSNAPHYMPGARKGLRLGDGSIVDGLVRDGLWDPYSNIHMGSCAELCAERFGVSRQQQDEHALQSHRRAAAAAAAGYTATEIVPVPRAGGTSSIRTLPTPPAAGGDGPAARGATIGTTGAIGGGTPPSLHGMVAAAAAAAAAAAMGPSATSGSYIWDDEGLAKMDPVRLTSLKPYFRRDGGTVTAGNASPMTDGAAALVVASYEAVERLGLPLLAVIRGFADAAQAPEWFTTAPALAVPRALQNSGLTSAADVDFWEVNEAFSVVDLVNRQLLGLPAERVNAHGGSVALGHPIGASGARILVTLLNVLRSRGGRWGCAAICNGGGGASSLVVETLDTQQVQQQQQQQQPALTRQQSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV30</accession>
    <name>A0ABQ5RV30_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanyl tRNA synthetase beta chain core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phenylalanyl tRNA synthetase beta chain core" evidence="1">
        <location>
            <begin position="4"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="94" mass="10011" checksum="BFF19646A3685946" modified="2025-10-08" version="1">MDAIQGLLDEVMGLLGVVYVEETAGRGTAPRGTYHTAASSDEPALIPGQQAAIFMSGVQVGMYGIVHPEVLAAFEIPYSASVLELELKHFINHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV67</accession>
    <name>A0ABQ5RV67_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003862</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052201">
        <property type="entry name" value="LRR-containing_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24111:SF0">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24111">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="254"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="616"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="660"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1144"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1513"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1652"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1723"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1850"/>
            <end position="1871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1888"/>
            <end position="2070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2091"/>
            <end position="2250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1145"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1168"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1573"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1723"/>
            <end position="1739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1740"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1757"/>
            <end position="1766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1853"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1888"/>
            <end position="1901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1965"/>
            <end position="1979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2122"/>
            <end position="2133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2221"/>
            <end position="2234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2238"/>
            <end position="2250"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="2250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2250" mass="229768" checksum="07A4F803ECA3D2B4" modified="2025-10-08" version="1" fragment="single">MAEEDPTPERSPSRQDARNASDGGAYEQPQELPSPNALHIIPKKHEMEMARRPAFLSCKWPEATDWCVDLASELQALGTTFMHDAKHRAKVHDVEEFIRDCSCVVMLVTPGFYHALANKPPGNRALKEIKMALQYGKTLLAIMHASYCGQNPLPSLMAARELTSDSGSEPAWTTEELSKVAHALPIFEFRGGDGFNGYQRELIRQVSALGTAARTTDTGESEADADNAVGSIGGSVVSSAVATSALGSVVMGTSGSDGGGSGSGNGGVLGVGSKGRSPGEAEAEEKRLRDVLRAAGLARRLPSLLEGHIRSLRTLEKALRKGRLSSKPFNWHDSWIQRVTQVLEAWPVMCTMPDWLRAAPEGLVPLLVPLLAQEESRDFDRDGKARPRRGALPPSTILDLAGQGPVVVGARGAAAVAAMIRDVASAQAAAAQRHQQHQQQLPSRRRLQLYGTATTMLTLTHVDLSDCDLGTGGAVDVLAAVASCPSIVVLLLGGNSIGDDVTKEFESIVRRASASTNGDGGDAGALTGLRTLGLDRNALTDAGAAAMVTLLHDFNVGVTALDLSGNVGLGTATAEALVRSLRGSGPRRLTRVGLTGAQLDEAALVALLPVLLSHHQTGSGSGTGSDGAAGSSASEEFDLSGNTVDSQALAALATAARQPQYNGGPRAMNLSGANLRMPPPSRRHTLSAELPNFYHTDGNSRCGGNSSSGNVSSLAAALQHSHNLEELRLSWCGLSGAGLRTLLWALGGGKPQHPLPKLTVLDLTGNKLGPEGVALLGALLPVNLPSLRHLVLDECGFGGRELGVLAGMFDVAPWVVHTLQGAICRILGDSVTGPARGSWTHRLDDSAWGDRSRPWVTFGDDAAVTARTALEGLLRSWLAMDAATAAAELKRLLLPPGAAAAAAAAFASPHSMAARKAAAIVSSSGSGRLAGLGCLLVHPVVQPVLEALRDLWEAVQGEKDASASMTTTMKTTTAAAGGMAAAPDARSRSPMQSPDASSAFRALDAAFLHLRSCAEVPGLTSLSLGRNSLAQEAGAPLGAAVSLSNGVRHLGLHGIVQPPPPAPVAGTGAAAAAAAAAAAQGGPLAAAAAAARDARALLRLLYSAALAPTLPNPFAMYRQAHHISAANWAQSNGGATSVRRLARLESQQSPSSNPDGASSGLAAAPEPRSQSDSTAVGQSFMPGLRSLDLTDAGISSHNAPHLAEFVRHLPCLEQIVLDFNSLSCSTPSGGGGSGTSDGGGNGGGTSSPEGAALLFADLAALPSLSYVSLNYACDTGAMFAVADHLLGLAVLPPSLSTSMSGVPSGQPACPNLRVLSLVGCPLDGVVARRLARALLHNTSLAVLRLGGGDPPEGVGPIGSRALGEALMSHRGLVELSLSGVGLTDNAARMLADSLPQLSQLCKLDLSYNSFTSYGLRALAEALLARNRNAVGAAAENRLSMHVYGNRVSEELETRIQALSVSPSMTHATDRMPNLRRYGAPASSRGEMGSSGGGVGLSGASPYTSSYTNYLGSVPSSRSGGGGGLPAPPSLVPSPMLVRAPVPRPAPAPMVQSKGPRCASSPPRGSTAPPAPTHPGLAAAAAAAATAPADPSRPLGSGKPTLLTAIPPPPPARRGSYPSEEGAASANGLADSLDFPVRMTYTSASKLTKIYGGGGGSPPLSPSGAGGVSYLDNGRRGSPNVPGQRPLSSPAPALKTLPMGISQMRAIVPGAPYAAALVNSGNNPLLNGNRNRPTAGSSSSSGGGGGGGGPASDSHYTGSGGGGGGGTLPAVRKSYPLAEGTSASGASETEDCNGNGDCSVTGNAGENANGGKGRKPRMNGASEHLMLRTKCHTPDWVNRMQEDFYKKVTAAPRPATPQGPQPPAPGPPSEHLFRKLLCHDSDWQRAKQMEQFMRETAPEPKPEPVPGAPVSAGMVSSVALIQRSGGAPRSTSLPTGTAVRSPYRSEPELEPACGLPPADPSEPVLEPTPEPETPQQPPPESESESALQRPAVELEDRSQQVSTSGGETEVGQAEAAGHAEPSGGAAPEDMVDSPAQLSPSPPPPAGGVFSLRAGLSGRACGTSGNLGSEESMEVLAVRNTGKKKAAIEVLRIRKAKDGEGSAAGVASGDGGASGDADEDGDDDGARTERDHVDGDGDGDGDGDGNVSSHDGRETDGSGEGAGALPWPESNPDGKDDGYVEGNVGDAAAVGSSREYEQRAADDPNGGEGDSESRAAPLRGRSHNSSTSSHNSSSASEDLGEMRVRDGEAEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVA1</accession>
    <name>A0ABQ5RVA1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_003420</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the thioredoxin family. Plant CITRX-type subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044182">
        <property type="entry name" value="CITRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47834">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN CITRX, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47834:SF2">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN CITRX, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="53"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI61144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="180" mass="19434" checksum="A761640DE3609F98" modified="2025-10-08" version="1">MAHAVAGAVSRKTVLITVSRQRVLPKCLPRSATATCARSTSHDLARGQGWGVSSRRSVISHAKVQKINGEELEVAIAGRDTTLIVDFFATWCGPCLLLARELEQVAEQLDGKVRVVKVDVDENPELSNLLKIQGLPTLVIIPKDNGKPALRTEGLLPAAQIMEIVSQLESGTLQAQPQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVC4</accession>
    <name>A0ABQ5RVC4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003909</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1470.10">
        <property type="entry name" value="Photosystem I PsaD, reaction center subunit II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053233">
        <property type="entry name" value="ABRA-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21715">
        <property type="entry name" value="RH04127P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21715:SF0">
        <property type="entry name" value="RH04127P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="1037"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="392"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="446"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="506"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="780"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="638"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="392"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="510"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="787"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1098" mass="119882" checksum="E94762EDBC22A5E9" modified="2025-10-08" version="1">MSARSERDGTTGPRGSRMMRKAEALGMETRNVSNAYLLPVLSSRGGGAGTLYGGASHTAIAGAGSYYETINLSPKAITAMRTTKMLQSLGQKVIFSNDPYVNSAFESLITTRVSGVPPDLAPDLTELISRKPPGGRLRPKGSKAAALKRAKRLVGAGAVPVASEVPFVDALRVCDLERDKGALPALLRAYVFNKARVVVPKLEEYCEAMGRLRPSPMLQAAALLMQAGYFFAAIKVVYSNFDALQATLTELETLISQHMDSGDWDTAAALWNWKDPDRELFLKQFKELKIISYYLDVRKQLRALKEQGVLALARVEREMLLKVIQDFRMQLAEDYAAYQAQLAKEAEERAKAKARLEAQALAAAEAAARRAEEAARVEARARAAAEVKARREAEMREKAREMERKREEVRRRAEQAKQRNRSASMQNSRPVEYVLMAGAAAAMFEEGAPHSNDGPLVDPDPEPEASDLTDVGTMTEPGVEVSEAVLAPGAEESEAIAVEAGDRISELRTSSSAGSRRASASSRQGSSSGSRRHSSARLTGDTVTSETGLPENLMMELDPAAAVSPSTNRPPVRVHLRPNSRGLRVQLSMDGRRPLSSQQLAAVEQPPPPLLGRLPVRVHLRPNSRGLKMHLSMDGTEQEELRRGIEELRRSMSQERQRANEAAEQIKHMAVKGSRPSTATPAIRLNINLTAGTTDEKLALMEKALEAERAEFQRRLEEERAALTAVLASEKSKLEDHLNEQKKLFDEERQRLLRDLYEQQGRDIDQQLLASNQSIEQLRQQRDRLRSAGGSRHSSGRNAAAAEEASRLEATVKMLEKKLDDELSHVRQLEDEKTRVEKERQALEQTQRMLEAQKSQAELEKAKAEQEKGALQEQIAKTMRMFQEQLAQTVKMAAMLQAQRILGAATGGAAPMGMGALGGNLMAQSSTGSIGLNTGGLMPQQSSMGLGTMGMNLPGTAGSMSSAPGVGFTGTLSASPSMGAAAAAATASMLPQPVAALPGDVDDPPTQDEIIAYGKYLGMDVVDDADLLYIAEWALTAPLPEGWTVHLDGEGNEFFYNAATNASTYEHPMDEHYRAYYRKMKEQKQLAKMTEAQQQTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVH1</accession>
    <name>A0ABQ5RVH1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="333" mass="36412" checksum="3EDECE62D336A06A" modified="2025-10-08" version="1" fragment="single">GGSGDGQQPGGEPPRAHMPYNRNPDDPAGYAESKVHSGTSAGSRFKEPSGKEIKDAQYLKVYQEPHSKVMHIQGANFNWDRSKDREAGQTTTATDNPTLFDTAGRAVHKAMTGEHYEGVPPMGGDSPGGQETPFIQAKENLQDKVNKAPAIASDIASRTEESVVGVLEHLSEAAESSAAGVVQLMRSGLGETKTMDERMAEAARRQEEREVLYDQDPAPRAPSGAVHQRRLDQMDQDADPALRRGTLVEKLLYPWSKDASTDDSNARAKAMSYNEYVREKEAKGEPYDPLGAMAHDREYDTAKLPATVQQVERSTVDLSTGERSGNNQEFLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVU4</accession>
    <name>A0ABQ5RVU4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">riboflavin kinase</fullName>
            <ecNumber evidence="2">2.7.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003756</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749:SF6">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Riboflavin kinase" evidence="8">
        <location>
            <begin position="232"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="380" mass="40625" checksum="1AAC0C40A1824653" modified="2025-10-08" version="1" fragment="single">MAMSCCFMRSSHKQLLAAVDRHAVRCLRSNRRSSVCVRAMTLKALPVEASVRPASPRTKELANRIMLAPGLSPGARAIVVGSGALPVIEVLKYLGVQDILAVDLSSEFLDVVTTAYGVATTLGNEPGVRVWRGDFTELPAFMGPAQVIVFTDEPFGTALNPRDAMVKACLIAVPGGCVIVNSEAESRRWQPKYPPTRDTLAALVSGLPLNIHSEQEVSNGYSGILQVPPNYRLRAPLCLGGTVVRGFGRGSRQMGTPTANIDPAPLKRTLAGMPPGVYFGTADAGSGPTWNPLGSDLCGPPRSWAKLDAPLGWPAVDSHVRKAVLNIGSRPTVNRGGEAPTVEVHILHEFHGGQEFYGSHLEVLVIGFLRPEIRFNGLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW94</accession>
    <name>A0ABQ5RW94_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NECAP PHear domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13228">
        <property type="entry name" value="PHear_NECAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012466">
        <property type="entry name" value="NECAP_PHear"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12847">
        <property type="entry name" value="ATP-BINDING CASSETTE ABC TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12847:SF9">
        <property type="entry name" value="NECAP-LIKE PROTEIN CG9132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07933">
        <property type="entry name" value="DUF1681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NECAP PHear" evidence="2">
        <location>
            <begin position="23"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="283" mass="30188" checksum="888686B96859F8BE" modified="2025-10-08" version="1">MASKSRLAKVSLPSDAEPGAELVQWSCREGYVYVPIPPAGSTGHRADKWDVDKWFKALNVELVASGDDMTVRLNDQQTGELFAECPLPSDGTPLTTAVEPVVDSSRYFVLRVVDKDTGKHAFIGLGFRERGDASSFTTGLDEYRKYLRRKQEADAMKADYERQESGEQGTYRDYSLKENIVIPLKIGNAKRQEAGGTSPGSNGSKAVDIVAQGVGALKLAPPPPPPQQQGETPNTQTEGSVDSPTAAAARDATGPSQGASPTSVPVGQDDVDEWGDFAGSVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXK2</accession>
    <name>A0ABQ5RXK2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004509</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNA polymerase II subunit 5-mediating protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23159">
        <property type="entry name" value="Prefoldin_URI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052255">
        <property type="entry name" value="RNA_pol_II_subunit5-mediator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT 5-MEDIATING PROTEIN NNX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111:SF0">
        <property type="entry name" value="UNCONVENTIONAL PREFOLDIN RPB5 INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="393"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="502"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="96"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="448"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="548" mass="55601" checksum="6B705E67DC741FC7" modified="2025-10-08" version="1">MSQELKAYLAAMQDAAREQKADVAAIQASVDEIARVRELLTRLPDKVRHKAMVPFGKHAFFEGELIHTNEFLVALGCDLRLESSARQAEAMLGRRQARAEAALKQARQQLEELQSRVEEFTTTLSNEDGEVLVDIRQDFEESEALLASAQVPPQAVATVTAAPLVGCADRVDSSIEPGNIAARDVDRRGDVEAISEDDRVFARLAELERLEADAGSGGGEDEGGDGGHGGDDVEGESKGGQESQEGIAVEGELWRAGQKAAVSTATAGPVAANQGHGSGVVQVDRAAGHGAGASVSGAMGATSVPSPLAKPKACTPLKKGFLLGGGGGGRRAATATTAEAAGLGAGPGPVRGAQATGGVTSSTGAVLLGGSSETGRGSGGDAGVSRRVTFADGSLTGKADDGSSAGLRQGLNAHGEPHHQQGGGDVSRVRPALKQGTSSVNLAGQQAKGERRSYMGDGERSGLRPGAAQVVALEDGGDAAMAMARAREEAFSGVIRERASEDVVEGAGSAGNGGPASSEIGPSAVAHGGQAAASKRVSKFKQQRMGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY71</accession>
    <name>A0ABQ5RY71_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="6">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_005254</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>indole-3-pyruvate + NADPH + O2 + H(+) = (indol-3-yl)acetate + CO2 + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:30854"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.168"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF78">
        <property type="entry name" value="FLAVIN-CONTAINING MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="862" mass="91089" checksum="000AEA56F637F509" modified="2025-10-08" version="1">MSSGEKDGPFTESRKLAVVVGAGVAGCQAARALLRAGYDALILEQGRGLGGVWRRNYHSFGLQVPFSCYTFPEFPYCKAPKELAERAEREGYPSGETVKEYIAAYCAHFKMEDHVLYGAKVVHLCALPNNRWSVRFRQAISPGQSPQLGPLKTMEADVVVMATGMYYSPYVPSVPGLASFKGTVLHARDFVSVSSLAGKRVLVLGAGKSATDCAVVAASPSAGATSVTTLFRQAHWPLPRQLFGLPFHRLVYTRFVAAMLPMYYTGQGSAAKRLKHTLMSPVKRLFWSTLQSHVARKMRLRGHLRPELDFVQDLFFGGQIQDGSWNEAVNAGRISAVQGSVAEVRPHGIRLTDGTVIECDVLIFATGYTKDYSLFDAATASRLMAAASSPGAEAGLHLYRSILSPWVPNLYFIGCEAASFNNMLTSGLQSLWMMHHVLGRLHLPGLQELIKDIQAQRNWRKSVMPPQHYCASVIMLYQQRYHDQLVRDMGHPPKRKRATRKGGSECFGVYTSEDYADLFADAAVPGLEAIAGAAPPPRLADAVVADAATLMPAAAAIGARLDLAVMPGTEGAVPEAAGVRRATLVPVCTEHSDAVAIAVAPAEDEVAAAGTSAQSNAFINSLDSPAKPSNVQPAARTSLSSTVLLAKTAPPPAVQRLQLSDAAVDSRGPAAAAVVAAALPSRDGRDGTAVFETSTTDGQRSSLGPMGLPAGSAMSNIVPSASVSVIDGGFGGAQSGISTVTGSVGVASIAGSAAAGQQGRPSSPRPVALLAHRVRLQVLHLINSNIGGGQHTGPAQNGEVEEVLEFRVASNTNFSGRVADSLATGSRGVQTNAHGSGNFCCDNMRHGAPNAYSELIGTLEVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY99</accession>
    <name>A0ABQ5RY99_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NFACT RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_005137</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021846">
        <property type="entry name" value="NFACT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11923">
        <property type="entry name" value="NFACT-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="6">
        <location>
            <begin position="618"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="NFACT protein C-terminal" evidence="7">
        <location>
            <begin position="1218"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="256"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="772"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="895"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1313"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="778"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="794"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="825"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="920"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="982"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1054"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1092"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1110"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1130"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1158"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1330"/>
            <end position="1349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1349" mass="140089" checksum="6994F4DB18BFECC4" modified="2025-10-08" version="1">MVKQRMSSADVAAEVACLRQRILGLRVANIYDLTPKTYVIKLARSGEEGEKVYLLLESGSRFHTTKVLREKSSELPSNFTLKLRKHCRTRRVESVRQLGVDRCMELTLGSGPAAVHLILEMYSQGNIVLTDAKYEVLTLLRSHRDDAKGLVIMARYPYPMAAMRLASRVTGQQLDAAAPGAANYKALVAAVLPYGPTIAEHVVMDAGLDPNVAVPLEDVSTADAPVPNDNRNADPAVIIAATDGADNATAISGVGLGAGGEEDGEEAGVAGGTGAAAADGGGGGGEGTSAAPAPRSGVLPSDVRRVLLAALGRLDDWFAQLEAGKVPSGFITLTPPGSSAKAGKKKAKGGKDTAVPATPSVAAAASAPLDPAAAAAAVAAGELVFAEFYPLPLLPYSGQPCLELPTFDDALDEFYSKVEGQRADIARADAERAALSRLDKIKQDQGSRAEALLHQAEECDLKAQLITYNLDAVDAALVAVNQSLATGMDWSALAGLIREERRAGNPVAGLIASLELDNNSVTLLLANTLDDMGEDGDEAAMTRKAVKVSVDLSLSAHSNASAYFDARRRHLVKHTKTVAANQAALAAAEKKAEAQLKQVRSAPPALQPVRKPMWFERFHWFVSSENYLVVSGRDAQQNELLVKRYFRKGDVYVHAELHGASSTIIKNLAPDQPVPPLTLQQAGCACVCRSRAWDSKIVTSAWWVHHHQVSKTAPTGEYLTTGSFMIRGKKNFLPPQPLVMGFAFLFKLDDSSIPAHRGERAVRVLDPDAASVAGGGPSRSAIGGSAAAAAAAGSDEDIDEEGREILDRDEEENEEAAGAAEASPPGGGGEGGSGGGGLTQDALVSSSSRRQPGIAATALVAPSPLDRFLEGSYDVPYGSTAPAAMAAAAFERYGLSHGGDVGDDNGEAAGQDGQATANGSGRGGGGTGAGGGRRHLSAKERALLKKQGTGGAGSTTAGARGTREDEEYTIVASDSPALPAGTGSGTAKGKAGGGGATAGKAPGAGPGAGVGHVKALPGTAEAPAGSSSKAGQRQPPPPPAPPSAQRGKKGKLAKVKDKYADQDAEERALALAVLGSAGEKKSRTDRRKERKERREAVRQEGRTALLGDEDERRQAIERATGKQFVKPEGGADDDVDVDDNDDGEVRVVEMEEEEGEEAAAGGEQADGVGAGNGVGEDGEEQAEEGPRADGATEAERAEIAALLAEENVEVPLADEDAARLSVLDSLTGLPRPEDLLLYAVPVCGPYNAIQSYKFKVKVTPGTVKKGKAARQAIELLIRGLDVAPRERDVMKAVPEMEAINALVGPGVKLSMPGLQKLKADDKKTKKARAQARVAGQAQGQGQGQAEDAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYX1</accession>
    <name>A0ABQ5RYX1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005447</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="134" mass="14323" checksum="A2BFC31031F12166" modified="2025-10-08" version="1">MNFGGPKTDTLGPKDQDVPPRPSAAPPPCPRCELVKSQCTKTIINYQKSHRKKIEALRAEVKSVRQQVESLQRQLGGYKAASRLAPAMAALAVAGWIILKRLRRPKNGGSEGGSPPQEQLVVSAPPTSDGSTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZF1</accession>
    <name>A0ABQ5RZF1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005082</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31437:SF1">
        <property type="entry name" value="PROTEIN SREK1IP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31437">
        <property type="entry name" value="SREK1IP1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="146"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="184"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="277"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="343"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="50295" checksum="F48D439CF3103AF7" modified="2025-10-08" version="1">MATRGIAQLKAQYNRVRTSTALQGNSMWNNVIGIENEQKGTNSAIEATLRAPSYGGGYKAQRMERAQQLIEASGYQPKATDLQGLLALAKSQGATGNATRGACKICGGLGHLTKQCKNGVSGHTGDIGDLDAAVAVASMRALLPDPDEVSSLDSADLEGSSLSDSDGPEGRGRGSRSDSDSGAARKRKRSSSSGKKKHKKDSKKKSKRSKKEGKKEKDKDKKKRKKRSHDSGSSESSGGSDSELDGGDQKRRRIRERVAAEDKGGPPLEAVPPRQQKLGEENGRDDCTAEARGWYRHKGTLEEDGQRRERRREVAVDEEGRHERVRGVRDEGQPIKAGHRRLREREWSGREEEYDRDQWREREREREEREREKERGRDRDRDGGSEARRGRGGASEREWDKGKEREYDRVANRERERERRSREDRAGSGEEAGYRQRLRDRDRSEDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZS3</accession>
    <name>A0ABQ5RZS3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026894">
        <property type="entry name" value="DnaJ_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052423">
        <property type="entry name" value="EMIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44094">
        <property type="entry name" value="DNAJ HEAT SHOCK N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44094:SF8">
        <property type="entry name" value="DNAJ HEAT SHOCK N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14308">
        <property type="entry name" value="DnaJ-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="161"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="403"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="426"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="564" mass="59636" checksum="731E9121A8A9BB85" modified="2025-10-08" version="1" fragment="single">SMDCPNSPSRVANNEGLGEPQVDFNDVFSLRKPRDARAGFASGLKSIAKGVLGGAAGLVAAPIVGAQREGVTGFAKGLAQGVVGAVVLPVTGVGVGTVQMVRGVINTPEAVMQASCGKLWDEDRREWVDKDMIVPLEQPENKAGGRGQGRGGFSRPESGDDYYAILQVPRDATSEQIKKQYYMLARKYHPDKNPGDTGAHERFQKLGEAYQVLGNEDVRKRYDANGTEGLDVNFMEGGSFFNMLFGSDLFEHLVGELFIATAARGGGEVGSTEMAREQGVRVQKLCVNLKAILKRYEDGEEAFVVAMREDAAKLGKASFGETMLHTIGKVYDMQADIAGGGFFGGMAAKFRQQGESVRSQFQAASAAIKVYQAQQRIEAWQREQERKAAVAAAGAAAGAEAGAAAPAGPDATAPNPDAAKADADAKANNGTAAANGAANGAAGTTPQPAASQGPSIEQLMERKKLEEAALPLMLEAMWAANVLDIQNTLKKVCKFVLGEEGVPKQKLIQRATALKILGGIFMEVKAPESATQADARKQMEEAMLRVVEKRAQQEAGGASPDADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZT9</accession>
    <name>A0ABQ5RZT9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein bS18c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_005184</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_bS18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_bS18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479:SF40">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS18M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="357" mass="37977" checksum="705E7268BAC27EE5" modified="2025-10-08" version="1">MYAICPLLLPKSITRPQNYNQNLRLGKKHLVRLSLRGCGTSSRVSRKILPIPFPFSSGLNNSVSSWRISKGEVGRLAGVEEDLASAKAAAAAAQVEAPGQEAATAAAAAAVGALQAGGSTAPDVSGGSDGMLQLRAQQQQEPGPEQQQQQQQPPEQRLLHPKYGYAALGGIVPSDEAPSTTTGLVGMLTSQNPRVNPRRRFVPGETYEPQDLNPFAPRAQGWTHPDMGAVRRPSAAEVMEKADYKNVAFLSRWFLSPAGRLLSRKQTRLPVGVHKYVSRQIKLARHMGLMAGEARLDKLHLARLREQELVEAQRRAAAAAAVAGTATEGATDVATVVVAVGGRAARAGLKELTDLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZW3</accession>
    <name>A0ABQ5RZW3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="837" mass="87967" checksum="1D20FA7DF4D2F5EB" modified="2025-10-08" version="1">MPNTTSPRHNSGFQYRLRFFHRCRSPISQCCDPGQIDRFQIQGSAIESTCGSPIVVALVRYKHDPESGLSRECQRAQFAPGAASFAIKAIVVPSGQVLSYMDQPLLQQPRLVDSKGRNLIEGPDVSPDGCIYATPLEKNGIAHLNNFCIMYPYTGGQVPGAFSGTGYSLGHQPPPAPVSKRKTSGGGGSRGKQHSPTSTRRVRGMVKGGGDAARLGGATEILQDSADVLHSNGELCLLVQLLRRDAQRPGRWYAYHIPHLISRPFQVVPQKALLASVQSSPAPPCLATAPPLMAASRIVTQQQKQEQEKHPSQPLMVQGCDFGLPRHSVAAPGFLGVSDPQLLVQPHRFMSPQPQRHMDSSAATTDFEGAVAMSDLRMYGTSGPTGFFPGDEMGLMEAPFSMIKQQPSQHLELPTGFIFGCSEPIIGLSGTRSIDRMGMSGGGMCITGTLQGTSSMAGPLTVGGGSGGGAVGVAVGVGGFGAERGLSSLSADSPSGAGSHRVLRARRRSLLSKDSSHDDNSVGGAPMSTSGVTATGLSALRTPSVTSAMDPNVNGLRAAALMTAAAAPAAPSPPYSPQQRILNAVSLGSAMEMERNQSLRRSDEGRSLPLPLQLRQAPSATLLEMNISDLLSDIHTQPLLTSEMMDLEEGAAKRVRSNTTTTSDVAAFMSDNNTNSNSGQIFGSPTGAHLLSAIESTVEASLGGTCGGEDRGCVGPNRDQAAFDIQLSRSDCWTTEKHFEQMMNLRKQVGACDAVNHGPGAAAAAAGGGGGGGGLGNNSRGSSASQLDLLIQMLTHQDGMEDEEQELLRPHQQQQELHVLSDAIPVPTCSVGDDMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S062</accession>
    <name>A0ABQ5S062_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_005314</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FtsZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF23">
        <property type="entry name" value="PLASTID DIVISION PROTEIN FTSZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="5">
        <location>
            <begin position="84"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="6">
        <location>
            <begin position="278"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="404"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="420"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="452"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="483" mass="49544" checksum="2E8245D751C00766" modified="2025-10-08" version="1">MIAAGSVTRKRVVCSNYPTSSLPNPRVYTTSISTAGLCSAPRASYGISARASTFTGALQPYRPPGRESISVASTGYTPFGGDARIKVIGVGGGGGNALNRMICSGLQGVEFWAINTDAQALAAHQALNKVQIGTELTRGLGCGGNPELGRQAALESEEALRRMVQGADLVFITAGMGGGTGTGAAPVVARISKEMGILTVGVVTYPFNFEGRRRAGQALEGIEGLRAAVDSVIVIPNDRLLDVASASTALQDAFALADDVLRQGVQGISDIITVPGLINVDFADVKAIMSNSGTAMLGVGAASTATTAPGGPDRAEQAAMAATSAPLIQRSIEKATGIVYNITGGRDLTLAEVNRVSEVVTALADPSCNIIFGAVVDEQYDGELHVTIIATGFASTYENELLSGGSSSQQQARQQRRAAHQAAAAAMNGAEPAIGAKAAPGGMGPAAQSAPLTRTPTQQPNPAQLPWSRPNRAKLDFLGRSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0J6</accession>
    <name>A0ABQ5S0J6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005798</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="429"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="445"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="47704" checksum="116E12CA8739F791" modified="2025-10-08" version="1">MAGMLQRRACLTKSGSILWDHLSLKATANSEQIVHGDRRLSGASTSTSSPAELGLQPSIQARRETAAVTISIGISGSYGSVTSCRGFTSPAPSALTASTFRGFSSAASAAVATSMPDPISARSPILNVIRSNNSITASTGSSQAPWPWVDPSRPQILPISVGKKQRQPVPLLQALSKLLGALAPEDRASTNLEVFIRFRLDPRRSDHLVRGSVVLPYGTGKAMQLVVFAKGADAEIARSEGVELIGDEELINAIVSSEGAAINFDRLIATPDFMRPLARAGKVLGPKGLMPNPKMGTLTTDVAGAIRDIRRGRLDYRLSREATVRAVLGRGSMTAEQLAANVGALVASLVENRPKALAGPAAATGAAGPAATAGGAAGAAAGATGAVPQATAAPTVSMLERYFMTFILKTTHSPSVHISLESLVEAVTQHRPTGKSGVQQQQQQQKDRAQELRADKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1C3</accession>
    <name>A0ABQ5S1C3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006534</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012879">
        <property type="entry name" value="CCDC47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883">
        <property type="entry name" value="ADIPOCYTE-SPECIFIC PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07946">
        <property type="entry name" value="CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046417456" evidence="7">
        <location>
            <begin position="24"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="435" mass="48001" checksum="0A4A0866EF789CAE" modified="2025-10-08" version="1">MRPAGALILCCLAVAVLYEQVVARDLELDGFDIPDLAADSFGSATNSQVGEKLSSEQCNEQCEEIKRANLPATPRAVVGGRRSTDPTSPHQSRKNYYLEAAFAVVLVFYIVVAIWGTKQNGKIADAFARTYCIGEGAVFSRQFAMCGAGEPNAAGFRTPLLKESSSLFKFYASGRRYCQGCLATLSLRNRADLLSWVINIFLPAEDLLEIDVYMNEANMPPMVLAVATPRQARALSERTDVTTFTKPLKLAPGTSSPTDPIPGWPGHKLQVLAEHNSLFYDLFSDPRVHSVFTSSSHTDKLRLFRSLLITSENSGGSHKQLLRLVFALPAADDMGALGPLLALMPLLVDLVGVHKLTPELKKRALDARSRHEASSADAEESRRRRIEAMQKRKLEKAQEEKERLARLAPDARRKAEEKLAKQAAKKSMKVKMIRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1F6</accession>
    <name>A0ABQ5S1F6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006755</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="260" mass="28909" checksum="BF369224FFAC969C" modified="2025-10-08" version="1" fragment="single">MDWRVLELLHVVDEGAYGVVLAVRHRLTGESFAVKAGKHASADPRVMYQTLREVKILRSLGNHPNVVRLADAFRSEEEHVYLVFEMAHCGLHKELERLPGRQLPAPTLKLVAWQLLHGLLHCHDHQIVHRDVKPSNVLVSRAITLCASSETITGGSDDGLLKYCTSAVQRYDQVAAEADHEGPTQRANTGSAATVAHAAAPLIKLCDFGFARHVYCSEPWYGERYSSYVVTRYYRAPEVLVGCKYGASVDIWSYGCTLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2K4</accession>
    <name>A0ABQ5S2K4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Formate/nitrite transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006742</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FNT transporter (TC 1.A.16) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000292">
        <property type="entry name" value="For/NO2_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024002">
        <property type="entry name" value="For/NO2_transpt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520">
        <property type="entry name" value="FORMATE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520:SF6">
        <property type="entry name" value="FORMATE_NITRATE FAMILY TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01226">
        <property type="entry name" value="Form_Nir_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01005">
        <property type="entry name" value="FORMATE_NITRITE_TP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="55141" checksum="6DA66273E036D521" modified="2025-10-08" version="1">MIPQETLAPPRQGEKDDSARGSSAGDGLPPGGTASPGNQDIEAGQGLPNGKTSADAQELIANGSPGCNETAGDLGPSLVAISNIAGLPQEADGLSGRAEALRATRSSSLYGKQRWAQSTKVPSRVFKQAPPHDEHHETAASIILTPKQTYDHIVATGVAKTQITLAKQVTQGVMAGFYISFSFMLCMTVGGQIDHIQHDHPGIYNFILGAVGFPLGLTVIMVAGADLFTSSCMYMITAWLEGRVATYYVIKNWIVAWWSNLAGCLIMAQLVVWAQLFAGPKSAFPIYLAHKKTAATFGATLVKGIICNWLVNLAVWMANSARDVTGKAVGVYLPVSAFVTLGTEHVIANQFELSLAKMLGSGIPLRTIIGSNWVPATIGNIIGGAFFVGTLYAGTYGTLYERMWLRTTQLYIWIVPKRTRERIADMYDAVYDRLYGWIDWDAITAPPNSGMGTAAPGVEATAAPPAMVSQKTFTHAVSSALVDAPKLLVTAAVAAVRNPPMTPFERKRTNAGLGSDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2L8</accession>
    <name>A0ABQ5S2L8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_007295</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21407">
        <property type="entry name" value="1B_UPF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18039">
        <property type="entry name" value="DEXXQc_UPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21400">
        <property type="entry name" value="ZBD_UPF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040812">
        <property type="entry name" value="UPF1_1B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018999">
        <property type="entry name" value="UPF1_CH/ZBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF364">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18141">
        <property type="entry name" value="UPF1_1B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09416">
        <property type="entry name" value="UPF1_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51997">
        <property type="entry name" value="UPF1_CH_RICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Upf1" evidence="15">
        <location>
            <begin position="111"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="476"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="C3H" evidence="12">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="CC/SHH/C" evidence="12">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="C4" evidence="12">
        <location>
            <begin position="179"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="930"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1054"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="950"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1063"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1088"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1114"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI64120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1190" mass="128029" checksum="AAAEFEE044617337" modified="2025-10-08" version="1" fragment="single">MAEPFLTQASQAMDFAFGYSDGGALGLTFQDYGATQDVHPGTLQFNDFTQGVSQALSWGAPVGIASAAQRVAQQAPQAGFETQLDGLATSFQQLRFQEAFDEVEESTVTAPEKAPEWACAYCGVHNPACVVKCLSTNKWFCNGRVHGSGSCIILHLVKSKNKEVQLHKDSPLGDTVLECYASGSRNLFVLGFVPVKSENTVVLLARDIPPNHPTIRDLNLDMSQWQPIVEDRGLVPWLVKQPGEQEILRARHLTLAQVNKLEELWKTRPNAGVDDLDEAAGPEDGAVQPVALKYEDSAQYQAVFEPLVKLEADYDRAIKESQSRDDISLRWDWGLNNKRVAYFYFPRDDNELKLMQGDELKLRHKNAGNRGPWEALGHVLTYQQSEEVCLELFTNDVPEDCTVGYSVDFVWRGTSYDRMRNALNTFRKYSASISGYLYHLILGHPVEPVTLKIPLPKAGLGVPTLPELNHSQLHAVKSVLQQPLSLIQGPPGTGKTVTSAAIVYHLAHSGTGQVLVAAPSNVAVDQLAHKMDQTGLKVVRLCAKTREAVASPVEHLTLHYQVTHMAVPEGERLRKLLALRGAQGGLNASDEKELKNLRRRLEMEVLENADVVCTTCVGAGDPRLSNFRFQHVLIDESTQAAEAECLIPMVLGAKQVILVGDHCQLGPVIMCKKAAEAGLCQSLFERLRLLGVKPIRLQVQYRMHPCLSEFPSNTFYEGTLQNGTGMGERRLAGVDFPWHNPDKPMMFWVQLGAEEISASGTSYLNRTEAAAVEKVVTRFLQNGMAPSQIGVITPYEGQRAHVVSVMVRNGAVRQDLYKEIEVSSVDAFQGREKDIIVLSCVRSNEHSSIGFLSDPRRLNVALTRARYGLVVLGNPRVLSRQPLWNSLLQYFKESGCLVEGPLTNLKPSMVQLHKPKRIFDRGSFGVGALTTNRYQPPEKVGDPLPSKVLASSSQPAQSSGPSQQLGLQMAAVGQAGATAATGAAGGAVNGGGAGVSGSMRYSGRFANGTIGAALASASSSGRSNGVATAVSSLGAGKGSTQTFMPFAMPTYSIPTASGRAKQGASSSRPDTSAGASQAASDLSLPGQLYTQATGGPGSTTQDLPATQLASQPPGAGGVAGAGGAGVRPTGLPYGFTADFSQTSYGLGGGVDDFNSQMDSFLLSQGFNETQFLDAATGEYYGGFGTTTQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2S5</accession>
    <name>A0ABQ5S2S5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006870</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33680:SF1">
        <property type="entry name" value="OS05G0489500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33680">
        <property type="entry name" value="OS07G0190500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="7">
        <location>
            <begin position="146"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="GRF-type" evidence="7">
        <location>
            <begin position="293"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="357"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="401"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="479"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="496" mass="49779" checksum="AF5761550C34F9F4" modified="2025-10-08" version="1">MADEDYSYDAGDVDDDFDQIEYRPPTVKAAAGESAPAGVKPYFSEKGAVGHKRAREDREEHHVDVNTPAAPRGRKARDLEQNNDQGGEQIGYYDAYQQPASKRATSATPGLPHAVSLPYNSTGNGAVTGGVDVNYSNPQTNLAPSCQCGQPCTRRVSNTGKNPGRAFFKCVKPQNEQCGYFKWEDELGSGGEGGATQTSRSGYGGGGTGAHNGGEGANGCGGGSAYSGAIGGGGHNPYVTNPYANSQVASSSGLPANQYIAQAAGGTAAYEGGAGAQNSTEKAGAEGDEFVKCMCGIDCPVKISNSANNPGRQFFACPKMRDDPTRCRFFQWADEIGASGGGSGGVGGSGAAAVGNCYVCNQPGHFASSCPQRQAGGGPGGNFGRGNSYSAGAGAGGGAPCYFCNQTGHWARDCPSKQGGGGYSGGGGYGGRGGSSSGPPQSFASRFGREGGVGGSYGGYGCGSGGGGYGGAGGGKGNCYKCGQPGHWASSCPNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2T0</accession>
    <name>A0ABQ5S2T0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NAD(+) kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007361</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="168"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="225"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="315"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="564"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="613"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="344"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="481"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="564"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="613"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1335" mass="136999" checksum="EE8A20B08DC8A6F1" modified="2025-10-08" version="1">MDLEDKAKATLQELLDVVKGYRDAAERSELEASRARVECEAWRYRAQEEHHRNVRLRRMLATASRAPLQFSTRPQRRGARLGINADGAAYQFGSMLAVTISALSSPATQRLKGNGSALVDNGLSPVSPSTAVAAATAAASAAAMSVFTAVFPRSSLQEAISMQGTSSSAFAVPGPGPGPGGLGPGSLYTAGARERQSGPWQSPGRQLPGAWDSADMEACPAPAAGAGVDSTAKNTSCSSLSSMGTGTGGSTRAHSRAGFKLVLRGGNGEPASLRQLTTYGAMFPFPAGNKAGISAGNGGTSKVHESYSACELAGSGTSNMGASGRGPTSSRNLQTAGPSAKTNIGGGYRGGGGSGELGTRARSVPSAALEAQLPMPLPGPAGSETTTSLSSTPTSRGSVTASISSDTETGATLQGSAVQVRESDRHPRREQKQHQDQRQTKIALPTERNAGLETAQGRGTWGSTVAAGDLTAIATATSDSGPGQQPEPGEGTDPQAVANTVSTTVVVNAATAPSSNKPPATSAFAARPYAVPITSVPYLPDTSAARAASLVAELAPELALGRAASSASGSSPCGPSTVPLQRVPSDPPSLYQHHFRAPRGPLETLTELPSPLHVQQQPLPGNTAPRQSQQQVVIPLPPGSGPVSRTQSVSYARSPLQGAALAAAVPRRETGPAVLLPHAVAAAVAAAAAQEELSWHAGSLHKQRTKLQDDLASIQQSSNLIRCNRATCNLAPGSPRHSPSLASPPRTGSAGAAAIAAAAAAAPLARWEAPLLPPLGSSTVLSPFGIAAAQEVTGAWEHELAAGGEIHDPPLPFSSSPFPTASSHAAADVTSAPAACALPPNDAGSGTQVAVTSPSIVSLEAGLPAAPVPPDLAKMPPQMAPSAPSHMQPIATSFAPVIAAPIPDGRDGPPADIPASGGDGVPSCQTCQAMSPEPAFVALYWVTPPVAALVVAKPSPGVRPTFERVLAWLHQRGVVTYVEPSILRDWPGLSCQPQRLQSRRLRHGVLTSLLAADAGVAAGDAGPGWEAGDLEEEDSGIMPGCDTGVPMLRSWPADEGDDCHSQMVPPGVAAAVDFVVVLGGDGTVLWTCHIFGNQSVPPVVPFNLGSLGFLTPFDPDNAEEVLRHIMEGGFPIMLRHRLHCHIVRAAEVQCALGTEPACAVGAGSEGVCGGCGESREWVVLNEVVIDRGISSFLTNLECYCDGSFVTHVQGDGLIVATPTGSTAYNLAAGGSMVHPQVPGILFTPICPHSLSFRPLVFPDHVSLCVQVPGNSRAQMWCSFDGKDRQALNAGDAVVIRMSAWPVPTVCSKDASRDWFSGVREGLHWNMRRLQAGAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2W5</accession>
    <name>A0ABQ5S2W5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045892">
        <property type="entry name" value="CrtISO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46313">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46313:SF6">
        <property type="entry name" value="FAD_NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="85"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="613" mass="65572" checksum="40BF832E6C5469AE" modified="2025-10-08" version="1">MIYISQRPFTHAHKHVCTLSYPSLRTQITRIFAHANATAASSKRAVACLAAAPLTTTLEDTGPPITQQVIDRSHETDFVVIGSGIGGLCCAALLSKYGYRVTVCESHYLAGGAAHSFEVAGYKFDAGPSFFLGIGGPAGDGSPNPLKQVLDAVGESVESKQYDRWIVYPPKGGSFPNIAAGAAYEATILQHGGPEALQQWRELDRAMKPIQTAAGLFPAAALRGDLGLALTAARFLGPEMLGTAFVAPLLMGPFSALVDKHVTHPWLRAFLDLECFVLSGMTARDTLCAEMAFMFAERNAGRTAIDYPMGGSKAIIDALVRGVEKYGGRLLLRSHVDEILMEGGRAAGVRLQPASGRDIKGGERPEVIRARCGVVSNASVWDTQRLLRPGTAPVEWARQSAATPALDSFVHLHLGIDATDLDPSMECHHLVVKNWHGLTDPQNVIIASVPTVFDPSLAPPGKATVHCYCAANEPYDLWAGLDRRSARYKALKEERAQPLWEALERFIPDIRRRAELTLVGSPLTHERFVRRHRGSYGPGISASGKESWPGPKTPIPGLSVCGDSCMPGIGVPAAAASGMIAANSLAPVWSHLTMMDALLPPATSARAATTQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3X2</accession>
    <name>A0ABQ5S3X2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)</fullName>
            <ecNumber evidence="2">1.2.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007309</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 3-methyl-2-oxobutanoate + H(+) = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10497"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:13458"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42980:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42980">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="5">
        <location>
            <begin position="56"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00051764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="377" mass="40319" checksum="FAFC81AA874AD13A" modified="2025-10-08" version="1">MQSAIFQCRPRLQGLILPVGNILTTAALSSLSGATMVQADQNNTLATEPLPGFKRLNLCNAVNDALVTALDTNYRAYVFGEDVSFGGVFRCTVGLLERFGKERVFNTPLSEQGIVGFGIGLAAMGHTAVAEIQFADYIFPAFDQFVNEAAKYRYRSGGSFHCGGLTVRAPYGAVGHGGHYHSQSPEAFFTHVPGIKVVMPSSPAEAKGLLLASICDPNPVVFFEPKMLYRTAVEDVPEGDYMLPLGVARLVVEGNDVTLVGWGQQVLVLQQAAAQLKKADGISCEVVDLRTLSPWDSEMVCASVSKTGRLVVAHEAPLTGGFGAEVAATVAERCFTRLESPPVRCCGADTPFPLILEPVYLPGVARVMEAVRGVVNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4Q7</accession>
    <name>A0ABQ5S4Q7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_008281</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="324"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="541"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="335"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="492"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="543"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="557"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="568"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="586"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="619"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="648"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="708"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="891" mass="91237" checksum="A4A3E9ED005889E0" modified="2025-10-08" version="1">MLEDSSLQTDVSQAPVDMESDDICWICLDGTKDRDPLINPCRCPRKVHPRCLARWQLQQAGRLEETNCRFCQSNLADWKASLTPENLKPDVQRVQPIMVVYFEGQIHRIPVKQGPEGLKEFTHRIRELFRLPDDVDISLTFGCKEPLSGQHLKLEGIGAFDAAVHCASVAAAERQQKLKSGGGSAATASSAGNGGVEAPSTAVGGNIGNAVGSPIASAAGNATGLRPGAADSDYLNHRHNHQNYHNQVERSASAAGAIGAVPSSVPVSTQTSSGGADATTTAGTGTGGLHAAASAPVLTHYASDSNLLATAAASATHTLESSVQGCVSLPEEPASTRSSSGGGVANSTDAAPSCPPTEQQQQRQRQPQGSASAAVAAAMAPCSHPVLSQRPSSDVTLATPFQLASSGVVDFGSRLTGGMGGSPQALENVDQHQHTLPLISHFITPPIDGPSMADSPLAGCLSEPYVSDDSPRAAGPNPASYPAGTPFACATPPPCLQGPSSPPSTPPPQQQLQQPAELACLGFPYSIPVISPYAAPMLPPLLTPTSSGGSTGPSLRRGSDGPLEHRPASPSLSPSPSLAAAAPLAPRDHYVACTDADGRSDANSSMPYKSHTYSHRQQRQQSMGSGRLSSKQLQQQHRTAVLGSSELTADNKSRSSSSSATNSNTCSTNTTITNAGNNAGTAVGPATPNHPVIRRTVSPTSLSRLPIPTLAPSSGPSLSSTGPSGRHHPYLNAAPDVDDVKAPSTVTAGGGGATTRSCNDGNRHTRRITDLHTDYDLPPPLSCATADDSAALGSLTGRLKFSLRAFSRKVARSLNFQKGGTGHGAACGAVLVAPGMMPSVVGSASVAAAVSEAGVLLPQADAEAEVEEITEIDPAAAASVSAIGAGSGHQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S563</accession>
    <name>A0ABQ5S563_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008075</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NIPA (TC 2.A.7) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF91">
        <property type="entry name" value="MAGNESIUM TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="283" mass="30416" checksum="43C3FD4C99435A70" modified="2025-10-08" version="1" fragment="single">MSQREQLLGLCLALSSSIFIGASFIVKKRGLRIAAAQGLRAGAGGFSYLKEPVWWAGMMSMVIGEAANFAAYAFAPAILVTPLGALSIIVSAVLAHIVLQEKLNMFGSLGCLLCITGSLTIVLHAPPERQLDSVIEVFQLAMQPAFLGYAVFSVCIIIFLILFVAPQHGTTNIFVYLGICSLAGSLSVMSCKALGIALKLTFQGDNQLLFGETYVCISVVVVCVMTQMNYLNKALDLFNTAIVSPVYYVMFTLLTILASIIMFRDVQSLEQVLTEGCGFVTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S589</accession>
    <name>A0ABQ5S589_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phytoene desaturase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734:SF1">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="13623" checksum="1AD969D64A50F786" modified="2025-10-08" version="1" fragment="single">GGGVAGASGATARPAATTAVDYSALVAAGREAVLRSLAEAGITNVRQHIVNELVIDPTEWRDRYSLAHGAAFGLSHGLTQLSVLRPGLADKQVRGLYFVGSSTRPGNGVPLTMISADLVTQKVMKDVKAGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5A6</accession>
    <name>A0ABQ5S5A6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L7a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007717</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="125"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="261" mass="29184" checksum="395B3C5F84D3BE27" modified="2025-10-08" version="1">MAPKGKKVAPTPAAAKKAAAPAKQTNPLYEKRTKSFGLGGAPKPKRDLHRFVKWPQYVRLQRQKRVLSMRLKVPPVINQFVTRALDKNAAETLFKLLMKYRPEDKKQKAERLKAEAAAREAGKEPEKKKPVVVKYGLNHITTLVESGKAQMVVIAHDVDPIELVCWLPALCKKMGVPYAIVKGKARLGTVVHKKTATALALTSVKNEDQREFAKLVESFKSQYNEGPRVNWGGHILGMKSMHKQKKRERAIAKELAQRAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6C7</accession>
    <name>A0ABQ5S6C7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LisH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008922</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="26962" checksum="558EC28D85015BE3" modified="2025-10-08" version="1" fragment="single">PLLPRPGTAARRPGTATAGTSGRPGSAYSGLQSSVQLLSNEILNDHPLIVEYLAKHVLMEGLALAEQQNGTPGRPGSSGSGSGGGTGRGPSFAQRLRDTTAAMNACEDVDPKKLAEQQQELIATSLTVRRGMAEEMVASASPNELCRQLVRLCEVLIKEVEACRTGRPASSGPGTVARPGTAAGLRPMTPGLHSSSSAPSSSVLTPAEVGELIQLRADVERLCRENKALRLENENMYWLVEEVKRLKAERGGSSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6P5</accession>
    <name>A0ABQ5S6P5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008590</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Cytoprotective ribonuclease (RNase) required for resistance to abiotic stresses, acting as a positive regulator of mRNA decapping during stress.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016685">
        <property type="entry name" value="Silence_cplx_Nase-comp_TudorSN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF2">
        <property type="entry name" value="STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017179">
        <property type="entry name" value="RISC-Tudor-SN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TNase-like" evidence="7">
        <location>
            <begin position="4"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="7">
        <location>
            <begin position="175"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="7">
        <location>
            <begin position="373"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="7">
        <location>
            <begin position="557"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="6">
        <location>
            <begin position="829"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="706"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1005"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="730"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1025"/>
            <end position="1039"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR017179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1039" mass="110768" checksum="5AF9E4C3BB6D6ABB" modified="2025-10-08" version="1">MATGWLRGVVKEVVSGDTLVIAGTTKGPGPAPEKRITLASLIAPKLGKRDGSSKDEPFAWDSREFLRKKCIGQPCVFRVDYVVEAIGNREFGSVFLNQQDNVAIAVVANGWAKIRVGKEQSPYLEELTRAQEAAQSAGLGLWSKDPQRTARAVRETVGQEDDAANALLSRVGKGGMVDAVVEAVLGGSSMRITLLPTGSVSLVVNLAGVQCPSMGRKAVAEAAPASEDAGAVVPAPAVVAAPTAASIAASSAAEPFAREAKAFTEARVLNRDVKLVLEGVDKYGNLFGTVLFKAPAVLPGGAAPAAPAPEEQEHLAEQLLKQGLAKCVEWSLGLMASSVALRLRDVEKAAKAERKAIWTNYVPAPTNQTKLSDNFTGKVIEVVSGDCLVIKDAANGAERRVNLSSIRAPRPPARDRPAEAWASEAKEFLRKSIIGRSVEVKMEYNRKVLTPEMMLAGDGERTMAFGNVELVPEKGEEKQNVSEKVVARGFATVIKHRTDEERSCVYERLMACEELAKSSKRGVHSAKEPPANRVNDVSTPGSAAKAKQYLPFFQRAGKMTGVVEYVLGGHRLRVHIPKEGVTIVFAPSGIKTPSRPQPAANGKPAVQGEPFAEEAFAFTREVMMQRDVELTVDTMDRGGTFLGTVVLVPQQQQQGGGAASRLFNLALALLGKGLARLQPNVDPSRLPDGSEMVRLQQAAKEQRLKIWESWTPEMDRDEGSGDEGDDSRPSTASAAGASNGHTAAAASSAAAGSFAAAASAAGSSAASTSAASGPRPAGGRSQEVLTVTVTDVADANEFFVQAVGEPRVAWLAEQLSAVSLAEKPPIPPELKTGELCLAQFSLDQCWYRAYVERVNRSEPMYDMYFIDYGNRERVASNKVRTIDAALSAVPPQAIPCALAHIKVPEAGSDWSADARAYLATLLDGGKPFLAHVVSRDRADPKAKHPKQRNGKVTAVVVEPETKTNIAVEMLLAGMARLPKLRKVKDPAAREAIQAMQEYEDEARKNHRGMFMYGDPGDSDDEEQAAGGGRFGGAPRGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S791</accession>
    <name>A0ABQ5S791_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_009254</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024862">
        <property type="entry name" value="TRPV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582:SF2">
        <property type="entry name" value="INACTIVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="514"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="372"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI65654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="640" mass="72271" checksum="FC9E25765C5C0867" modified="2025-10-08" version="1">MAKVAPRKEQPSTEVEDIEEAPAPALANGFADHMREQLLMACETGDVDLLTQFLEKAEREKVLSVRDSQTWPLLAIAASRGHQELVVRLLNFGCPVDIQDEYGSTPLYRAVGFRQNPIVKLLLARDANIRHVNKDGNNIIHNSAYVCNDFATIQALKAGVDPTQPNAVERISLLDMVEKQPHLALMYFDSKVKPLSNYVQYSKMLYDFTGLDYKDSQGLMDSPMETMINLPASNFLLSHMLVKQYLQWQWRLYGRRNFIGNLVQQVALLVLVTYIAITSLYKWLRSDGEVYEYCPCSSGADFMLDVVRAAAELLLLPLCGYAIMKEVQEYRKSGNTQVRVRGAGLVSRLLRLLFRADIDIHQTRAAVVAVPRYLLDIWNLLDLGSLLLLVALASIRVAAIVRHTRILSPRAESYVLAVLLIGVWVKLLALAGVFRSTGPLLRVLRRMFINVIEFSGLYLLFYISVSLALFLILALATDLEDDPYGTMLGSFMGTFQITLAAADYTVYEKDAFATVLYFFWTILSTLLLLSLLIAMLSYDFQVGVEVAEREWLVVFGGYLLKTQRLLGDAVVQRLKVDDLQRCHEMGGFQTPLGEAKTNLPAMHTAIPAKLFVVWEDTTTWPPDFRQKMERGFSYDDEDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9K2</accession>
    <name>A0ABQ5S9K2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051571">
        <property type="entry name" value="N-CoR_corepressor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992">
        <property type="entry name" value="NUCLEAR RECEPTOR CO-REPRESSOR RELATED NCOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992:SF39">
        <property type="entry name" value="SMRTER, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="928"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1021"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1185"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1542"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1597"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="946"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1031"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1048"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1135"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1158"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1554"/>
            <end position="1572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1641" mass="166127" checksum="93E9A6CBAF75BF50" modified="2025-10-08" version="1">MQDPLSRLAHTAAALSRRASSHLRQQLPPSPHSSSLQRQQQRQQQQPAAGDEAEPVSNAEGALMAEHAFFTEDEPSSEIEEAMGCRGVSASRTIPFDTFPRQLPRSHIGSGGELDMPPASATTAAAATTAGGSTCVGGGGSACGGSGSGSGGAVVVDDGGDGGSGGGGDNGGAAVARRYREGKDGGRAASPFGNCGEDGSQGGTGAGSGGGTKRPGSRRPVQFWGTFNDWWRAELERTGKRPSAEAVNSWYSAHAAACWGPHAPSLEETRTHAKCLRSVGSIREYFRGYRARKKARQGEEPFSAGASNNGCNFPTGGGSCTGGVGAGLQNIPNLAAFSSLLPQLSQFGPLGLSPLQVVAAPKYGQTASRGAAATAATAASTVVGFQEMPFLGLGADGALLTSAGVGGPGVSRCRTAVGSPFDSTSMYGMSLSDRMPPDSGLIPLLPPPPPLQTDGVCGPFQPTRMDNDSLMKKDAAASPAAAAAAAAAAAIALPPVALTVTTGTGRFQRKANPDTTKRPLQSLAITTAMPTKAAVNAVHGGGENSSSEGGGVTGPAGPAAAGANHAAAAGCDGLQDSVIACREAHETAVCRKAAAMAADDSAFSFHIGGVCLSGSDGGRSSGSTPPLLHFGAPCTASGSGSGSSHRLFDIQPKQLLAAMPHTDAAAVIRITPRSATPVAIASAAAEAAEAAEAAAAAAAVSPPGSVPMMPSSLQKLSTHNVQFPVPFTRPGDIAAIAATCCMDTDGGVQYKLISPSAVAVGSSGDGGGCGGGCGGGAMLRSAGSDDINHCDRTAVAVAAAVAAPRSPAPQFGFQHPSCQAAASRDKYAAPLLPHYRAGGGDADNDGDGDDPMAVAHLLAASRRALNRMTESGSGSRVSPFQLLPPSSQLQPLEDQPQQVVRQLEVEDELCTVPSFSLLPSVPTAAAGAPGVMAQQRQGKAASPQPAGGGCGTGSGTDNGGRLNTTTDRMSAAVTSSPGMLAVARGQQQLLPAASCHTFSVAEQSQSQSHSQQLLVTPRWTDASSPNQLYHPYHHRHHHHQQQQQHHQHQQEQRQQQRHDQQHMPRRPAPPVAPSSARQCDQAPVAANRSMTGPSNQQPEHYEEQHHEQQQPQPHATHLHPGEISPCGGGYNSPAQQQQQQQQQQQQQQRYQHQETQHYGHHNPNLHHHHLTQHENTKVFITPGSFLASGSHRQTPPGAQHSNGGGQGHSDGQQSAILATAAAGAVVAAVASSTAAAASANATTTLPSLRSLLARPDAEIYSLILELRHNQLALLLLRAVAMVRQKKAHEERLMDELAQVLEQQGNLQAQLHTQRMETARMYRTIMAMRDARSSGGGAADGAGASGARGGGGRAMAAAVVGSGGGIGFTSSAASQQPRRRSSLGDEELGLSSAGLDLATLQNRVAAAAARRASAGGLAEAVVPSPRAAAGGGGNGAAVGIHGSPEFTAAVGLVSRDGAEGYGIWNHSHQHQPSPACTPGAPVAERISAVAGAATAPCPEASGLALAGGMAADVGAGGDGSDFAVRTSVVHDCRSPTVVAANTVTGRSTDPTGRGLLLGGGGGGGGGSGSGGGGDGSCGRGGGDGGGCNTCGGDSKGIRMSPSWEGRYTTTASPPTAVDSRTKRLAHRCRATAASYPSLDAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9Q9</accession>
    <name>A0ABQ5S9Q9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_010342</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="22"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="774"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="75"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="190"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="270"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="449"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="622"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="673"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="864"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1076"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1180"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1223"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1364"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1434"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1602"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1737"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="218"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="480"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="628"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="639"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="673"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="698"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="864"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="964"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="983"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1252"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1364"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1375"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1456"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1531"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1653"/>
            <end position="1665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1771" mass="183253" checksum="A4D4B99713852B56" modified="2025-10-08" version="1">MDAAMAMAPQSAPLNASEGYCGKPDKACNNCNTTRTPLWRREAGELLCNACGIYYKSRGVHRPVELIRAQLNQTSRRGCGNGSGAGGENSRARALPGQVTRKSARKRQERVMYGDEPAEQRQQRSHSTAMAAKGTRQPSYSDMASGSIFHRGQPPLFRYDGSAGGAGGALGISYGFGQYHYLSPDEDPRYDTEHGMYDEGCALSGRRGPQSSGSVSDQGDDGGSEDLREGEEAEDTRSLSPTFSLGPELNADDDAGTVAAFLLRMRRGRYRSRQQQRRKAVQQRQQYGLERGMDGGALDSKPPARLRSPSMVFEKRCPLRSCSDDGSRGGEAEEAEAIKGEQQADFVRAVKRRGSQEGTGKQLAVAAWPPGVSGPPFSAVRVSPAAAAAAPQVKMKVKAGGLRGSGVLVKADEPAVALSPFATAASGVQGPDNGVVAAAVALVPSDVEGAPTRRKRHAGEAWSRSPDKDPMAAAPPPPDNRPRAADQATPERHDADDGDGTDGEDYCHRPAKSSRGQWERSGGAPAAPPAPGAANSADAANQAVMLKLFSQAESARLLAAMEPMQRFLLLAAAGPGAGPPQGLDAGGGTGDADGRCDVLRQGCHPFSSYPELLLRQLQDAALSGEGGKSSSAQANQAGRQQPSHQNQQQLPRQLPVSVQEVIAAVMAPARAAAAAEQPHGAAEPPFDDSENQTRRGTSSAPASASAPIGFGVMGGPVAAPEADGVMSEGGWSPVRGGGGMGSAGLGIAVSGSGGGGVRASSACPPPPPRAPRHHKGPLLCVNCGTTQTPLWRRDRETGSTMCNACGIYKQTHGFDRPVGGRHQVPPQASKRCAALRTMPIVRGAAASDTLTGRTGRVEVGVAAPAGVPNSSAPGRASAPRPPSAGGLSAASARAAIAVARGSQGLSPEDEELSADGVGPGGLGNCDTFGGSSEAALRQEPELSRRRPSGVESAAVGIESQQLRVDSEQGERGAGHQCCGGQEPSVQLEAQQRQVSEQSQGGESPRLTAIPSPLSVTQVAGGGSLGQTAPAVARGCDAAGTCVASSIRSPNFMSDESWTNGVEGCSLLQQQLTLPRHATQPQGQQQQVGESLGEATGMSEEAGPMQPARRGTPPTPVEIPAYEVVPMGHRDSQADVTRIEEPMTGETTFHAGGAAVSASAVSAWHEASRGSDVGAIDPWEHRRRSSSCGQAGPSRGLAVGPHGAAGANPPLAVGVGCPLVPSLSSVEATSGAPSRADASVALLLPPPPKRKRVEAAEQERDLGGGSEEQGGECAAQQPRLGGVAVAAAAAAAAAAAAPIDSDAADVGQSPSVQDFAQQAAWLVHMAAAAAAGSSHECPLPPPRREGNDPQRQLFLMLLQRQLEQENGRGHGERSQAQRQQPQVNVPPVGPAAMLQKLVLSAVDPPVARAAGNAVAQSPSPNALALQRQRQDLPFKQELTPPSPPLAAATAAGGKRTSAVQEVSSRGANPSEQLEPGGTAILQGPPQTPRVQSHPAHQLQVPPQPQPQPRAVDGSEPRVVRVTPHPQTQMQPSSPPSPQQPPKPQHLAAARVVRPPSAVIVPGHGAQQPLCVRIHAKPIPAHQLHQLQHLRAAGVSLGHLVIQQQQQQQQQQPPQPQHGADRDKEQQPQVCSVKQQPQPYPHPHQQLHRHPYPTPTYNLNHHSQHSAVQGGQEGEARRQQQQLHRLLRARSTSEQGPAAAAAAAAAAAAGAYLEAAEPGTAAGGSRPRPVVVPVSVSGASGGHPPIPVRVRSPSTACPGGEPLGMALSTIGWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB90</accession>
    <name>A0ABQ5SB90_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF1">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="457" mass="48262" checksum="53AA8DDC318DE78D" modified="2025-10-08" version="1">MDRYSNFNDCASSQPATNRMGLSPGVLAALERVHVSLRKAMSTTMCLRTSSLQAIAERLAADGLLVTLRSGLGVGIESPDYLSTLRHSFLICQEGSAGLCVIVDPFFRDQFKVAAMPQTSNYCAAVSKLPECFVGTIGTVNALVCLLTGTLLQESTTVGVELPPWRSKQALIGKWLPRRFADTVFTPPSFVSLHPALRSSIHGAPLSTSPTSVQSWPSFANRIDNELGSSNSSASDACSSDDTNTTSRCGGGACGRFSPYRPRCEPYTVISGFSTPSAEAAAPVVPHRATLGSSDAVFAVRPAARSALTEQLISASNFSRAVQAEAEAHWQRCFEMLQVPSVDKPRHHEVAQACVYADSAATGSSNSGGNRDPLGTHGCSGKAAATASLTRIGSDDVLKSTHQEDVLHSLVVSPVHRRTANCAANLKQGGGSLRLSAATLKMLPKVYTVKLATAEAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBF2</accession>
    <name>A0ABQ5SBF2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_010766</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="24"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="24"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="114" mass="13266" checksum="1A15149870D03AFD" modified="2025-10-08" version="1">MPRAVKEKTAVAKKEKKIKDPNAPKKPLGAYMWFCKDVRESVKKENPGMSVTEIGKRLGELWKEVSEEDKKKYLKQAEEDKERYNKESAAYNKEKIKEKEKAAAEDDENEDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCX7</accession>
    <name>A0ABQ5SCX7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="423" mass="45877" checksum="8CBE9EFCCDB9D98B" modified="2025-10-08" version="1">MEEITGRRFPPASAPTTRAIGPILQQRLHLHIVPVLRHRPVPRAIVLCRCSDSAEMSQANPPPSAPFPKQGTFQALVSELSPLSPLQLQDVAYQRASELLDSRFLFWLRDQERRPELGAADRELISRLGQELTMMREWTEEQSNRKLLPSLASSLAYSNLRRWQEETGSGAPEVVPGVDLEQLYRLGEQEERKLRDSLEAGSSGTKPKRGAARTGIEEFTKHNKAYADLAAGAMARVRQRLMGYDDEGPSAAAAVLRVLLQEMASPEERANFLPDAFTPPGLQVLPDTAGTGASSGGVHKLSFAVTTPEALMTAAMERLAALAEDEGGKGVLHSTATNDRAEAAAEEATSQGAASKAVAAGSGTSSLCQQGSSRGQVRHAVLPSGEDEVTALKELLELIEEYDRQVYSRKTPLERMGHSAGPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD64</accession>
    <name>A0ABQ5SD64_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012160</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF8">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="21"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="172" mass="19420" checksum="DCAA885F800E7FBF" modified="2025-10-08" version="1" fragment="single">VSFQTRILLLLDAMVKHELAVSFDVVREKNLDQLKLLNNVIFPVKYADEIYRQCMACGDLTQLAFHNDILVGAIATRCEKQHNGKAKAYIATLGILAPYRSLGIGSKLLARTLAACAHDPNIEEVFVHVQIDNDEAIRFYERHGFQRGETVKDYYKKLSQPDAVILTKKLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDD7</accession>
    <name>A0ABQ5SDD7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="EMBL" id="GLI67915.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="651" mass="67629" checksum="E7AD99B33DD86005" modified="2025-10-08" version="1">MDARMDAQTSAAARPLTRGDLNRAKIMILEEIKSLSKMVTDRVMTHLSRTEELYRTAPAAGNLQVSQLAQTSTFLDPEPVRTGLSMDGLHTSCPQLEAAHKKRPGVPIHVPALLRPPLMTCDDLEELLAQRLAHDPLLPLRWEQELQARLLAQTPTPGSTPLHSTGASPSITPISTSPPSPLLGLCRSMSTHICFSNADVDAAAPFAVRGLPGGSVLSQPSRGVSGWEAISDFNATCLSGPGPLSTGTAIAPSSLSGVRTSSGVTIPAGLEIPGFPGPRRYLPTDGTLSPSSCQRNLNESIPHEAAVTTDGSMDHFGGGRQEIYEELSRFTHPSSSCWSQSSSAGSAERDALYADTWGFRPQSMKSNISAATAAAGEAVAAAANEVVGPRRELPQGLVQEILRQLAMVQSSGGTSAVYKASGTAPGRIVAGAGGIATEVCSGGQSSSRIPRPASAESGPDREVHRNVAAAGDLGHRTQPPTLPVTSSRSASATPVYNPGFLPDRQPRQRQQPSPQQQQQQQQQPGQCISNSGKPTNCLLASSSSSSFSHSEARISSGEGRSADANTFAAGRHLDGRPAAGCSASTSSSSSFATLPGSSAGNPSLTGSPLDSNTGAIASGLLSSSQHLAMSPAFCSSCRESYSCLSYGQPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
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    <name>A0ABQ5SDY9_9CHLO</name>
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        <recommendedName>
            <fullName evidence="4">DUF3598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">VaNZ11_012426</name>
    </gene>
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        <name type="scientific">Volvox africanus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
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            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="PANTHER" id="PTHR36025">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (DUF3598)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (DUF3598)"/>
        <property type="match status" value="1"/>
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            <begin position="47"/>
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        </location>
    </feature>
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            <begin position="391"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SET8</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="2 3">
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        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
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            <taxon>Volvox</taxon>
        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="GLI67969.1"/>
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    </dbReference>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="3780" mass="378057" checksum="AE8AD59FBF0E1394" modified="2025-10-08" version="1">MQGQPAQEAPASELHELLKEIKELKAGLLVDSVTHDNPTFEGPPWANQQAGMRLDIPTDDLEDSLEDLVARDTGLQYILSPQAEAQVAQSSRNTESERSSPRRAGSAAPGIGRGRNLGLQTTVAVSGTPGTPASSLPGRHGVAPPAPAAAGSVHRTPLASRGVTSAFPSGSTSAATPVASAESSVVALTPAAAVPSLTTPVTTTMRSKIGSRIARAGAAVAAGNTAASRTPVGIQSPALRSGSTVQLNAQSPSPAAGAFTHSAAPTGTLPVHGTTAGPAAFTASPRAQQTSTPALPRTAREKVAAAVGALGTIATGGRYSPRSIGKTGAPAGPSPASSTLGARSSTGSGGSSSTGSAAPATTTQTPGPSIRKRPPQGGTAAPSSTPARKVPGSAGLPGGTATRSVTTGTQSRTTATPSALGVAGKIPSRSVNSATASAHSGSAASSPPPSPQRKRTAVAAAGPSTGAAAKRPQHANLRPPAAAAAAGASVSGAVISTDELECETSHRSLTLPTAAIGPDMMAEESLEDLLADDPWLAASTKADASCGTGVFNAPGDSSPRLQRRTSDELLRPLADAMAEGTGNSDWMDEDTDAAMNLLMLGGTDDAELEAVMSPVAVAAAVAAAVTPGAASSPSTGSRHGAAAWIPNTTPSPRVLGGLLPESSATEILAAASLRASQEPIGSTVCKVRELLGMDGPSSQQEGIPELDSEELWRTTAADADKLFLQYGTNNLHNDTDEVFPATSTAAAVAAAVAVPREPSVPSSPAETPEETQAAERRAVLETATPNPLPLSHQGVPTARSPVPDATATLDGRDSWTASELAAAVGASHYDILETDDGFLSDVLAAEAAAAAAAVVCGPGSSLAAGAIVTGNVHPLDAAGNVSTSSARRMLLPSSSSSSFVLDATLAMEANMISRQLAGGPPSLRVHGGSEDAILECDELSSTTVTPQSVAPAATMTAMPSPECGVAENLYAAMDDAMEAVYQDEIASPAPLLATSDSDREDEGTAAEGYRTDAPMEGSKESGPAHVPPSAAASLNAAAPRTAAVVDMDLAFEGTSQDEADLGSVMAAADALQDADEALTAATATEVADAVMDMDQKQSAVMAENEAAAAAALESTFTVSPFAVAMPEAAAAIVGTPGKAVGMVGNAAEAWTELQETAAVAAPRTAEDVDMAAIDNDAPLDPEDVQLLDDDESKVAACGATTEDKPVFLGTGPAPDANEVMQHAAAAAVAAAAITAAETVTVPVAQTAAVSAHSCAAATTAAVKVGCDEQQEEVESEMQTPAVAVDDAAATAAGATDTLRDTQLSSLPGLPVVAAAATAAEGPQERTANVLEPVEDSRYAQPLLGGAVASETTALAKAAQGRKLQQVEGTPALMSGHSSAEMTTDLRGGAQSLLAATATRALPLVSTTSAATAAARGEVETEALQVGLDTPMDNANEAEEVAIPADAAQPTTAVTVLEAGPVEEPQLDGAGAPMDEVAAAVAAIQAAVPVDAQLLMAAAGAELSVEEEMVVDGCYGGVEEPVTAVAEGGVRPLLEAVMEGATGPAGELHESQGVSMDIAEEPEPATIEPRATQAVADSVEMAWEEIRARDLAQAGVVDALARAFPADVSGAAPDSHGMAAATTAAAAPSDCPLDVHGGDAVAEDEQPNDTAADVARSMPSPIVGSAKVSSHGFSQSAETEAVSAIADSEGEGARADSDRRFTDAELHTGSGGELDDQMYDAEEDGFEVGQTQTAFQEQLPPYPEFLTVPEHLIPNGIAAATTLGTEAVPMATVGPETDPSASAGMDHHVEGRSLMRVATETEAETPDLGTSTGGMIDIPFDRSARITLSTVNSHQLDDVDYGIDEDAELDTSAKRSGGPDDDEDGVSSDLRHKAPAGAGASLLAAAAASGAGTGSSAVQHTTGDGGVADANADDGSTTATARKRARLAPVGPATEEEIARSVSGSGLGLAEFYGSVALADVAREAASGAATSGSVAGKTSAYMQLDVANLIGAQAASVPMAGAETDSVERSDRGGPQTESSLDRSLELVSTTQGTCAIPESDADEVYNAASPTSTLSSAEAATMTEVSPTKQDACGETVPGGPMPATNEAELRGGMHEDGGYASRPELVTATPASTWLAPQGAAIAMSQPFPAGTSRISIGVTDEAVLQTPAVISSRTAALPISPRNAGWRSAGPAVSTVPEAPSSSEQQPLMHAWQLLVPTSPGSGHEGEGEGGPNSKGSLSVRRSLRSMLSGSSRLSRVSSHRDEEPATERSKETDAGDDGASTPGMVSPAACIAAIDDALDAPGHAANGAATVHGGDIGQQPLGTGPSQVAYCDVKAPHTGVDGPIDANVDAVVPAQHDAVGVDISQGASASPFKSIDGEPVRHVEEVEDEELTPAWKVAAAAAVAEAGADTNSFAAAERSSLGVRRSLRSMQVASTGSRLKRSSSHSTLSQSAAALPDAPFYGDLAATPSCVGEPLPGAAPSAAIQTSGSCTSPASGVVLPAEGYGSGDEQQVSPAWKVKAMPASAGDSPDVATATSSVPLGSQPSFRSSRAGSSRLRRASAPDCDDLHSADMAGDKGAVGDDAEVAAFEQEDPRDSAAAETVLTAAISGGPTLTGDVIETGAILSLGRARFPTCQGDNASDGAVGSRVRQAAGSSHSADGWTQPPLLRGGSVGRVGSLTNGHSFRHADVGGGGAGSPTRAGTSENGSMAGAWVARLSSRRLNPDAVGSVPLPRRSNSGGDPNVDAMRPAWQILPLPMSPGSPSGSIDGTGTGYFSASGGAGITSPGTTPSAANILASMRSNSRLSRMSSGIPEDLLAELRLQAHAAVGHLQGSAHDEDSGGGWADMNSSMEQSATHERLPLDTAVPTADGQVAATTAAGDGAGGTLHAWKVLPLRATAPTSSGLALGDGHRAQAAGAVPMTVPRVLSGGRAIIPTSRTGSHSSRLAVASTKPTILQVSFGPTSPSASSPKAEALPEVSEEEEEVEDEPSSAVSTPSGVSTPSAKAKATPAAGDGEDSSAVLHAWKLVPPSPGVSASASASTTSPGGGMAAATTGLATVHEGNNCVAAFTIVTPQRSHPTLGSRPSLKSLGSVRGSRLSRTSLGVEDYVDESYTDTPAAAGPGDSDDVESGDSAIESTGITEPRQHSADRIAPANEANQNAVAAAAAAAAAAVAVADNAGDSVLLHGTVAAEAEPPQLSLRSASERSSAPAAIGGMPQGVLYAVDALDSRPGSGALKFVASRSTPNHRMGDFPHDDDMAFEEGVPVTESPIVRALNSRPSMRSSRSSNSSRLSRVSSARDGGAALLADIREEDGEGYTRSVGGASDVAGASAGVHPETLAREERLPTTEPFIGSQGVLSAAEYDADDIGGAGADSPSGLELLGSRLSPTGGAGMRSGRSGSSRLSRMTSQMSEAGLEDIKEEGVDAAAGGQEAVGSVWASPVVADEGCDSSAVVMERGSSMRAPPPAPSAGAARIGSPVSSGVPGPLVGLRSAQLGPATAPWTSAPEEGAEACPLLPAWKVLLPAGIGNASPNAREADFPERTSPSASPGASRQPVLRSQRSSSLGNSRLRRVTSGVDEEPEDVVEDGSDPAAAAVAAIPGRRNAASASGTALADVTLASKGVILRSGQVHAIPGVDSKDTTHDDNLTFANLASGRLDNSPPSNSVPVSAPMGSRDFSDFSGSQPHATAAVPATTQSPLVASGMISEEEPSPWSGIPQSPAPAESSPQKVLQVADGSQPSRPAHQRQVATDSGANSDAGVETGAKSFQSPRTPQTPTRKGRGLFSCLVCFSAPEISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF92</accession>
    <name>A0ABQ5SF92_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="200" mass="18498" checksum="83A9776FA057C45F" modified="2025-10-08" version="1" fragment="single">GGGTGGVSPALSTNNLEDLAADNCGGGGAHGGGGGGHHVSFAALPPRPPPRTRSNASMPDITTGPTTGGGGGGSSGAAAAAAANGHGGVGGGTPGSAGGGGGGSHPASRSQSCADINTAVAAMARFGSGPRVAGGAVPSDGHSAYGISHLPHLHPHLHHVSGHVSGRASPSVRSTTGTLYGGGPTGPYMGKLESIASSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGG3</accession>
    <name>A0ABQ5SGG3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Prolyl 4-hydroxylase alpha subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_012646</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-prolyl-[collagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:18945"/>
            <dbReference type="Rhea" id="RHEA-COMP:11676"/>
            <dbReference type="Rhea" id="RHEA-COMP:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF229">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prolyl 4-hydroxylase alpha subunit" evidence="9">
        <location>
            <begin position="203"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="442"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI68286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="467" mass="49516" checksum="ADED168B4DF9BBD8" modified="2025-10-08" version="1">MRCPVTDSVKCRGSRTAVFQYLPSPRVWSLAGATHRVPTILCHVSPPQNSGSAPTGPLPSSTMPSAPDAADAASVLGAAWDPEGLFSNSTPLNISAGEQMGDLIARRERERAARRGATVIAPAAPAAQTSPADAAMTAAAPLLPLPAHARPLWPLPDITVAAGGLRPEELPMEVLSEVESLLARDFMAVDLRWPGLRLLHLDPPVITVDNFLTGEQCDQIVQQAELSGRMQASRIGASNVSSLYGNASSSRRTSTGMMVTPGVAGPIMDTVVSELQFRGKKLLRAGDGPAWGPSSKMPHSGQYCYEALQVARYDMGQHFLAHEDGFPAHLAASNGFQRHATLLVYLNDVLRGGATRFNYLGLAVQPVKGKALLFFPAFSDGTSDVRSLHTAEDAVDTKYATQQWVARGLPKPTTIKPLAAAAVAKQPQDAEARQELQLEQLERAGRQGKGGKTAMKGGKGFGSKPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGQ6</accession>
    <name>A0ABQ5SGQ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013481</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="11981" checksum="5023DEEBACE7F75A" modified="2025-10-08" version="1" fragment="single">FFAIAMPKDLEELEGKLLLVEQQVEAEATEATTVSLYAGQERFNNRNSKAKRDDGGNNNINNNDSGFRGRCYNCGQRGHRSADCKKQKEDKPFCTHSKKTGHTLAQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH18</accession>
    <name>A0ABQ5SH18_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="222" mass="23562" checksum="777BB6811103A8FD" modified="2025-10-08" version="1">MEGQGAPFPILPSAIQICGLLGALYPHFDILDDKFTFRTGRLQLDRSLHAMSLGSCILLLGFYCCQFLPVLAPLRKEYLGALALTQALHFLLLLARAAIFPGSLEADPAGTLLGLVAAACVSVLVLNENLAVPRDPSAASSKPEATARAGVAILVATHALWLVFVLMFLLPYFSSLLPLQVPSQMAYGSIAAVLTQAAALARLGMFMSRRAAVGLRSKQKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH32</accession>
    <name>A0ABQ5SH32_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013819</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="45"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="322"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="392"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="436" mass="48239" checksum="A3BDC3B3EE7DA161" modified="2025-10-08" version="1">MEDDLYGCVETATVAEGSLESTLYEGLFLGLSSFGHHEAYLQHKVDELEGLVRERNEELHTLKGQLEDRNQQLLNVTAERDVLLRNISCLYNTAKEEMARKQAEINSLRADIATARQQQQHAQRPMQRDLHLCCPTLPAGVEAIRSTGQASTAILAGSKRGRDAAMDASTQGNNGLRSEAEKADAKRTRCHGIRDGPAADNGVKEDNGARQQNRISPQTQPGKKFVTTSALVEARGHSSCQIRRSTGRRRSCEGGASDDRVQRPVIHQVSNHRANENGNGHHESHGFAIPMSVGELNASTRGSEGGGSHRGSNHSRAGYLEGSKDNIRERLRERDGPGDFRIPARESHESTRCGYENRQRERDPQRSGRHANPSDVDASGPRFLERSAGTDTGRDRDLGRLKDRGFLIGDRARELHGSERSGGRTRQEREWPLARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHG2</accession>
    <name>A0ABQ5SHG2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013889</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPIRAL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039613">
        <property type="entry name" value="SPR1/2/3/4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33403:SF31">
        <property type="entry name" value="PROTEIN SPIRAL1-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33403">
        <property type="entry name" value="SPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="3">
        <location>
            <begin position="39"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="190" mass="20183" checksum="5592A5634E01B623" modified="2025-10-08" version="1" fragment="single">FEIPIARLDLSWLSIAVYRISASTTRPVKMNSTEVDALLKPLTIKDLRVQCRMRGLSPAGGLEALRDRLKEKMVETKDFSFKTESGEDLTVVQVTAGQASKDVAAGKGQNNYVRPAGQNVGNFITDRPSSRVLAAPGGASQIVFGDSDAPKASSNNYSRPAGQNVGNFLTDKPSSKVSAPPGGASQIIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHH5</accession>
    <name>A0ABQ5SHH5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_013975</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="470"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="697"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="704"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="742"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1304" mass="143915" checksum="C35D680667574565" modified="2025-10-08" version="1" fragment="single">MGKDRLDEPLDLHNYSTWKLRFQLLCAELGYSRALLQAPIAEADVIISDKVKSVMAKNVKNHHLQTIVRAANAKAAWDALAATFASTCNTRKVALRQELSDFKMANGEELPIYISRARQLQSDLLDVGHAVADTELSTIVIKGLPSKYHVVTTALTMREGELDFTQVVARLMAYVIPDEDNNESSSAFITHRSGGKFNPTSRHQTSKQRSKSNAICHYCGKPGHFIGVCKKRLADQQQQNNGRGGGNLQRREPTKMAYAVGSESRLDAWHLDSASEWHITYDASELKDLRAVRPEEKFTLVGYDGSRHQPTHVGQYTMWSNTVKGLQIELKNVYVVPKATVKLISAGIFDERGATILLGNDKALVQAKGRTILRADKVGRLYAIRHHAAGRSVGKGRSCAAVGSEGSKAALWHRRLGHLGYKSLERMCREHMVTGMDLDRASLEAARSRICEPCIYAKQTRGPFPSTGHKAAKPLGLIHLDVCGPMPETLIGGSRYVTTLLDDCTGFSTVAFTDTKDAVRKKVKIMIEALENMTGRRVKEARTDRGTEFLNTVLGAYFGEKGIIHGTTVGYSPEQNGAAERLNRTLLEKTRAMLAESGLPQKLWAEAMATANLLRNISPVVGASVTPHEAFMGVKPDISHLRVFGSTAYAHVPKEKRNKLQPVSKKGVLVGYEQGRQYRILINNLISTHSTVKFDESTVGDRYEDSDEEDVEESGGVPLPGGGGDTPVNTDMDQGAAEVNHGASTSSGQTAAPQRHAGIPAAQFPRMQNTAQSRGGNGGDAMTGEGPVQQQNHNNDGADVNMQERRYPLRERKRLIEWRHEPNGRVTFGRINAAIEEEVPEPASYQEATDGPYAEEWRSAMDEEISAQLTNGTWELAKPPPGARILPCRWVYKVKRAEDGEIERYKARLVAKGYEQRAGIDYGELFAPTSRFASLRMLLAVAAAKAMPIHQLDVSTAFLNGELEEELWMQQPPGYESADPEQACRLLRSIYGLKQAPRCWYMKLAAELDKLGFKPSVADPALFIKKDEKEIVYLLVHVDDIIITSEDEAMIRRVKTEIGQAFKIRDLGEAKVFLGMEISRGAHGEVKLSQRRYIEELLQRHQLVNAKPRKTPLPPGTRALPAEDNNKELVDNTKYRALVGELNYLATSTRPDIAQAMSVLARFMGNPSKVHMGLALGVLRYLAGTKELGLCYDGDGDLTMVGYSDSDWAGDPATRRSTTGYVFLLNGAAISWNSQLQRTAAASTVEAEYQATAAAVREALRLRKLTRDLGLRTQAIQIRTDSEGSMSLGNNPIMSVRSKHIDVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHW7</accession>
    <name>A0ABQ5SHW7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TIGR00341 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014193</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005240">
        <property type="entry name" value="DUF389"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992">
        <property type="entry name" value="AT15442P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992:SF9">
        <property type="entry name" value="AT15442P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04087">
        <property type="entry name" value="DUF389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="54242" checksum="908EA62D08103A3F" modified="2025-10-08" version="1">MRQLVVTVPVDYEDHVIEALQHVCRLKNITRYSDGVTSRISCYVAAQEAGIVLHELDKVGCGAAYGKVAMLPVQLLKPLPNVRDRNRHLLSLVAKKAWHVRGRMATEEIYTGVASGSELSFDYLAFVVVAGLIAAVGLATNSSVMIVASMLVSPLMGPILAFSFGTSVRDPYLIWKGVVAETVGVMLTFLVGLIVGLAVCIWTGVAHGYQWPTGEMESRGKVSSLVVGVFFAVPSGAGVALSTTQGGTTALVGVAIAASLLPPIVNCGMCLAFAAAGARRVGWQAGQFFRVSGMSLALFVINVGCIYSVCILFFWIKGIKKVRKQLSLYRDLPPIPMKAGPLQQVKADVGVSIASPQMPKHATTAPAVLVGDDKGTAPAGTDRSSAGCGNGGGGGQTPNGGKVVIKVADASAAGGENGGRPGTQQSGSAPGSRHSSAGIVRRTTGLFGSLWRIAGSGLGGGGSGADNTATAPPREGRSVEGAPGQTVRGWGRVRKLVLEDKALQKIPEPGDVESLNSPRVHNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI10</accession>
    <name>A0ABQ5SI10_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021434">
        <property type="entry name" value="DUF3082"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35733">
        <property type="entry name" value="OS02G0307800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35733:SF1">
        <property type="entry name" value="OS02G0307800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11282">
        <property type="entry name" value="DUF3082"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="343" mass="36796" checksum="F033BDA851748DCA" modified="2025-10-08" version="1">MTDRQLVSFHEYAPVASLLLTKPLRSSASRMQSIPSTSGRPAQFWLRPRLGIYLPISHIRRFNGLAKVNIRCRQGSPAPAENENKLGNGLENIMSTMVLYTMTWPAGVVSAAEAGVTYNPTGGDEFLKNLAGVAYILLVGIFLIRLFRKRAAKAKTMRIASQAPVGPTFFDALREKVGAPKQAKATPLNAFIGSCQALVLAYGLWFFSTKVQGAIEVQALPDGYTARNIAITVRTIIIGLCYLATFIFAANGVGLAGLSVQLLLFPGSIPEEEDSEEDDGASRGPQLRITSRPEEIRAAFGVAERLGKREAQKAVATAATPATSLVEEPAGDDPSLSSSTDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIF7</accession>
    <name>A0ABQ5SIF7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014425</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055164">
        <property type="entry name" value="EDR1/CTR1/ARMC3-like_pept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14381">
        <property type="entry name" value="EDR1_CTR1_ARMC3_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1490"/>
            <end position="1746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="374"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="499"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="607"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="655"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="706"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="774"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="883"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="918"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1011"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1367"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="527"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="618"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="676"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="715"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="776"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="817"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="933"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1011"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1045"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1115"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1144"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1395"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1414"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="1517"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1772" mass="186034" checksum="5A68C0D85599570B" modified="2025-10-08" version="1">MSGEVLRRALTVLLCGRTVRKESLQSVREESLHKSAENLNVAGAIPLPAVPVSGLSAPMQSHYQSAEFIYQPSEEQALSIALAQSVADQAAWQAARASGSSQAHQVILQRSAGEALSKKLWQDDSLGYDDVITDGFYDVYGDFPEVCEHYNDFPCLEFLRQVRTGAGDVREVVLVDHELDQGLLQAEEMLSEAMAESTPQDAIARIRVVANVVCDRFGGTYDSEAVLERFWQVSSAKEKRRNRSAVLLLCRLDVGGLRHRALLFKVLADAVKLPCKIIRGQAFCGADNAANVVVLVEGSEHIVDLVFDPGRLISQDQFASMVQIRTLYAGKDNWLHPANTSVSGANAGSGTGVETSGVSISSTSSAGTPAGITGFTTTTISTNSTQKGPVSSGAGSLPQGSHPTAVGTGINGINIVPPVSTLADVPPAPHSSPRATLSRSNMFIPQGPVSGTYSGPGPVTGAGLQGQVGAWGLGPGHVHGNSSGPAIAGSRVRFTITDLPKAGNPQPQGARGELRHAPPTVSLPAPGTGPTPAPLPAPPGGSTATAGCGGAQAVLGVPGRHSMTSLPGKAKSMMDLAATTQSDGDGQASASAPGVDLIRLDSEPLSDSLHEAVPPPERTGTPTGTSAPGPRGTPALLSRRQNSFDQNGWVKFSGSFTRSKDDAKPWAHGGPLGLSPLSQPSGEHTATAGNAQALELSTSFQAMGAVPSPDGTRTGGSISSTPNIAGSGAPSAGRSVLPAAQGTPFCNVTTASPFAHFQMPSDLSAAAFGDPAAAGNTGGSGGGLSGRTSHGGSEGSLPKVGVQPQHNSGPQVLLSQPPQPSSQPALPSPPPSQPRSSTTSPFQAMQMGFGSHLPPASLVSAFGGVPPLQPQPMPGTMIHTQAPAHLQHQGHRPPQAPVVGCGTGGSMAAVNLPLPRMSSVPYAGQPQAPSPPQVAQQQQQTLPSQPVRQYAAVSHDSMHRMYGLGGGAAVAAGGTGFYTPPPKSADGVCNTGSKAPSPALSTPFTAATASSAHSTAPGSAPADSVPFADLSPFNVGETVASCGSGRKDEKPERDREQWERGREREQRDWEREWGRERERDRERDRAQSFFADLSPFNTSSGAGGSGETRDVTPARRNTSGSSPQVQQQPIVELPSSDNSRETTPRVSITNLSDAQGPPNSGGGVAEGVARDLSLMQLGAPAGGRQEPNGGRPGSMHQQHLQIQQAFGPMSAMGPAAIGAHLAHQLPHHLAMAYYANVAQVNPYMYVQTPAAIVAGTAAMLPQAQNYLQGQSAAVGQGMQGWRQVFDPAWANSWAASQQPNQYMLHTAYMQQQQQHQQHEQQRQQQAMMAVATGMAGQVQPGQALGLPGSGLSVVSSTTTTTMHVVESGASTSGRSQPPTGPGLLPSRALVATGGPQLALPPSTQPQLPQPLPQPQAQQPPQQSQRVADMAHEMTVPSARRLERLQVPSATNPDAGGGAGLASPLGLQSSLSVSMPLPPSYRDLEINPDELTFKNRIGIGSYGEVYRGTWRGTEVAIKRFLEQNLSQVTIKEFRDEVMIMSKLRHPNIVLFMGAVTQSNQLAIVTQFMPRGSLFRMLHRTREVLDPRRRLNMALDIAKGMEYLHNCKPVLVHRDLKSPNLLVDKDYTIKVCDFGLSQVKMNTFLTAKSQGGSPAWMAPEILRGEPCDEKSDVFSFGVILYELVTGKEPWDELNPMQVVGAVGFSGRRMSLPTDLAPAVTELIISCWATSPKERPSFTQILATMTAWSELRPTEAVIQLQQEAARARQQRAAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SII3</accession>
    <name>A0ABQ5SII3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BFN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013560</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Bifunctional nuclease with both RNase and DNase activities. Involved in basal defense response. Participates in abscisic acid-derived callose deposition following infection by a necrotrophic pathogen.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bifunctional nuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.690.10">
        <property type="entry name" value="Bifunctional nuclease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036104">
        <property type="entry name" value="BFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003729">
        <property type="entry name" value="Bi_nuclease_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15160:SF1">
        <property type="entry name" value="VON HIPPEL-LINDAU DISEASE TUMOR SUPPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15160">
        <property type="entry name" value="VON HIPPEL-LINDAU PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02577">
        <property type="entry name" value="BFN_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103256">
        <property type="entry name" value="Hypothetical protein TM0160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51658">
        <property type="entry name" value="BFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BFN" evidence="4">
        <location>
            <begin position="88"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="254"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="315" mass="35067" checksum="15668C3F2E188F8E" modified="2025-10-08" version="1" fragment="single">MAVAVFSTDQPRIFLSHAHATPKISFFRSNSIPINLGITVVSRTVHHLQGARPNAPSRASRQIVQAQCRYTGYPFKENDYLRVELLERPPYGPMKVLPATDDNAGYSGLLVFQREHEEALTVDTPIMEVYVAGDSACNIYAQLHGQKAHRPVVHDLMYNLLARAAEVHRGQWQLLRVAIVALDGDIFVGRLFFGDAATGTVHWDCDCRPSDGVYLSLRTQCPFYVARTVWEQAASPLRLSKVHMIAVHEDTMTKQQEQLQASQHHTQEHQNGASDSGTIASNGIYEHRRGGGGGTISPTAYEYMAIKPDDMDDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIJ4</accession>
    <name>A0ABQ5SIJ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cleavage/polyadenylation specificity factor A subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="606"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="714"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1180"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1247"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1316"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1575"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1644"/>
            <end position="1663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2120"/>
            <end position="2141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2248"/>
            <end position="2280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2895"/>
            <end position="2926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3059"/>
            <end position="3120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3324"/>
            <end position="3395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1189"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1247"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1599"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2122"/>
            <end position="2131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2912"/>
            <end position="2926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3059"/>
            <end position="3073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="3324"/>
            <end position="3339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="3354"/>
            <end position="3368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3378"/>
            <end position="3390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3409" mass="345335" checksum="A037B274CA6FFEBF" modified="2025-10-08" version="1">MAGTWLSKTLVPSGVVDHVVCGSFVESGHPGQAQVILLHAGSSLEMLAIGASGAASSICSQPLQAGEAVLDLRVLRGGPTNGDGHPDSDSLILLVDGGRVTVLRFSESCNRFILVQEVQLVGPGTQPRFMPRLLAPSPSGQHGVAMAAFQNRILYLPPASIAACTPAPTVTVAAGESATSVPSEDACTVPRAQVQASISGAGTGDVAATAVPTARYRAVLANTPVPYIHRLPLGDAVPPPVLTAGPIVGAAPRDLRRATADALMASGSRLAASGGYLPLQLEPGCGAIWDLAIIESPCLSTDRDGAVAADGGLEGRGAIKSGGKGFDGGWVGSAGPGHRQERQIAIGSGSGSAGECGFRVVALVHRSSTDAGEVLLLRWIPGAPDLLLCDLVVRLGAGGPSAGLSSTGAIGSGQGQREGPCAGLESKVSVLGAHDPTLNSDLEAGAAAATAGTSEKIAEQVQRRPLLGMAHVLRATPPEHEEAGLMVLGTHGAVFLLAALLVRHALPTNRRSDLRTVGSASGSAPALEAASPLSPISAAAATAAAAGAASAGGNSSLSGMAAAHPSADGRAGADGASAAASGAAAQPPVLSLEGLMAAAFASSTTYREAGTREGPRHSDGFNSSSGNGEGEDDDDNNDIGGPSTVAGKCRRLEGLAACLKFHVGRSQPPPAHHLQRAPEAASGRGWAYLRFAETQFAVATGTVAVTKNHVIEGDGDDGMEVSDGTGSGGSPAVRQAGSAAAVTHSGGRRSGASAMSGGGGDSSWRSGLCRYVGEEDDLGPGSRATWVFLRRCGLQRATGGPVRHRLLEQLDRMASMIKLYRSVAEHAVFDAATTGSGGGTAVGTASRAEWAAQEEHLQRFSAQPASQPNMLSLSQLQNQAQWMLRHLRREELAAQLRGVGDSTAMKDLRRAAGAAAKAAAAVAAAAAAIEDVNLEECQGNAARPLPPLKQAPPVIGVERLPLVEPHDDGHILSAAEWLPPMLSPATCPFGCNSIAGAPPKGPRLLVCAAAGPALCITLAAAGAPPGASRATAAAAAAADVIETTAGAQAAVSDRSFHDNEGTGGEVALGLKSVAAAQEGTEGNLPVVYLKAIRCLMPSGVSAGVSIATASTASPAASGRCATVRPARALAALPSWSGGILVWSEEGGDVLILRHRYKPLPQLPQQHRRRQLQRLGLQPRPVALPTQPSQAPPSPTPPAISPLLTSAFAADAAAGFQMDVGTCAAMTATTAPWSFACSSGSSLPGDGAIAGPSAAGGSQPPPRRRQGAGILGLTPGAGAWCQDAVASASGSIRGSGAGGPACRTGISAELRCDGPRRRVQTAPGSGGTILGQPRTPKATVRKAPLALSLRLLGRAPQPRPMVDFVVTETAGISGHGRVLQVTGAVLGLQPPEGAAPGPRGPKLQVLRGCLTAQVVAHMPSQGATPTGLWSIPLRTPIAPAGVAPGMPSHAGGVVAPVPNPQPGAPPAGGSLIVLSFVGGSRALAPVLDTDFAAGSGSGGSGFGPGTAPSPLEVGVMEPSLRDVTDALQLRHWEPTVAAGLVAEGVFVQVTPSGILMASLDLVSYPASGAAATVAVTPSGGSEPLDDGDGPGVGVGSWDAPLPRSSWPSPKSSRYEDRIPLEQMPQLSQPLEAMHAASTRLQRQQRQHHREQQLWHGDGSLGPPYTHPTATGSMLISMGMLEGKQMPGAAAAAADRLPPGMAATSGGGGGESRGPSINGYSSMAEGMGVMALASDMQADDNAQGAARFAEALSSRQDGIPGAMSNLAHTQDGYAAPVFYGPFTSQCLQSMPPPPPPPALGPHALDACVGGNFQGRNDAAVDAHDGDGTSTSAVEAEVSHGGVAWWNATALDVTTGAVAPGCVVLVRRLDRRLTVLGVVQQTTSGGGDGSGTSRLSRKRPRSTLVLRELGFSLPLDCEPSCCALRHVLPSRSPSPQPRPHSTFRRSPSSSSIAAAIASAAATVPAAAITDAVDSGAPGISVGAGTATALSAGLPPLPPRPGLANQGGSPVLAVAAGGQWSGPASVSVGGASGGAPAAAAAAGTVHASSWRYQLLVGDHAPSIQLLLLTYGSSGLCCTRLARVSTAVPPGLGPCGGLTGPQSASVPESVLFIAPAPVPTAAAAVLGPGGSSGGGRSSSSISTDRRELRTVMPSGLPGTHGSESAAMAPAAYSVSPPDGSSSLLCLVGLRSGVVTVFSYGVAGFSAPAAAHQVPSDDMEDLGFPVALSGATGANSVGGNGDGTEAMGSYTLEASKGPPAPEAATAAAEYARRSRRNGGDGREGGGGAELPGVLRLVWRCSLEQVPVRLASLAPCRPWQVLAVGSAVHLLELTAPSGRLLCSPLLPSTGNWIGTGSAATPHQPLPPPSKGLEVLLAAPLYLPAAGIRDVSGAGVTAAIAGESRKQRPVAPSPAGSLPLELALVVLNDGSWRLLSLQRLSRLGRQPPSAMPLGPDSGGTMSGMAGACPATLPIPGTVPIGRPRLLIHVPQLFPHRPSPGHAYRPSIQATAPLAPCLDGSTGAFGSEAGAVKGGNRATASHVTSPGGEGYLVLLADDAQGSRVQQGGSRYVHVLEAASGRQVTSFDVDAAASGFLAACACMWQVRREADDGGGEAVLLGCRAVLPPGPRWQQPWWGQAGIREDQSVLDVAEVAIAAPPPAAAGVELHNGHVGPGAAAAAPMDEDLERGLEMVREMVGEVDAVVDQERQRAPPRWWWQHGLGDGAEAAEQNDARGPRAIDDQLEAGAEGDGDAEGPMDGDVAVVARRLWHLVEAEMIGQALPNTRMGSSCGAAATGRRCRPPTGVPHMLLVIGCTGQGGGEVMVLHMVPALLQLVAQQHGSGAGDGVSSTAAAVVRSPNANTPMEDWFPWPLARSRTALQRWLGWSGWAYPGGEVTTVAVEPASLPPAADNGDGPDALMGSPTTPSAPTTAAESRLPTTRVVHLQVVHRLLLANPVTAICPYNEETLVVAAGRRMLIYTMRDGRLHRTGWVATRNPITVMSACSARSLLACTDGVTGVMLYTAVYLREDSVSGALAGPPVGLRLIAADAIYRPITSLLLMPSQSLPSRQLPQSLPLDQSGPSAIRTGGGAGHASPPGEAMDAGPAVNERNGAREASGQAIPAEEATGMNGGESSAIIAATDSLGRLLLLTPGPATLLSCRNLITLAAVPCPDAGLRLRAHLPNPVTRYNMARPLQPKVPGLLLAGLSGAVHQVRPCPDAAVPLLSLLERALASHWAARLPTGGRHRSAQEAEVDALDEAGRWWWWCHQGHPGVLDGDLLEQLLDLPRPAKLQVLRRVPAEALRAALAAASSGRDTEYYEAVAAADTKLQALAQTQEQGQGQEPSNDTVMQVDGCAAGPPQAVAHEEEAFGDRPREAFSRNQPQQLHGGTSADAGSSTGAYDDDDLDSVLQVLQDVLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIL9</accession>
    <name>A0ABQ5SIL9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013850</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sel-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF147">
        <property type="entry name" value="SEL1L ADAPTOR SUBUNIT OF ERAD E3 UBIQUITIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046497248" evidence="4">
        <location>
            <begin position="29"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="768"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="799"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="803"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="828"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="922" mass="99806" checksum="ACB2A0CD2218CA29" modified="2025-10-08" version="1">MQQAMLQRCVYLVLALFWLDFCPTFFLAAEKPSPSLSPRTLAESFYKHAVKIRSSSSPSLQEQQKAVELLVAASGFRSVRHGKAFPFNGTLPCQDGIECDKQPKQQQEGEDGTEDMVLEGLKPLVSLSTGGHSGAVRELAKAFQDGWGVSQDMRLALQLFRAASNQGDPEAQGYMGMFYSMGLDQLDSWDAAGIVAFGQPKRPEALLHYYFGALGGDMSSRIALGYRHLMGLGVPQSCWTAASYYQSAAEQVSILAAGLDLSAPLTEGIKATTIKNDAVKTAKDGPAFSAMDDTAIGTEASPGIATRRQFASGLLPQVDRIRLYHQATHDVRNERQREMVQYYQFSADRGNIEAQTAVAQVLNYGTLGMDRDHEGALLYYKRAAEANDVTAMAHLGDMYANGHGTERSFEKAREWWELAARRHSGVALFGLGYLHLTGRGGVTQDFDKAFVYFTKSADQIHSEARPEAWFYLGVMHMKGYGVRRKSVQRALTYFLLASQTGHLLAQYNVAMLHLAGRGTARNCKAAMQLLKTISEKGPAAASVQLGHEHFFRGQYSLALLAYLRAADLGLELAQSNAAWILDRGLVSGINSSELAVALYQQSAAQSNVHSLLCLGDAYFYGKGVKQDWVRSAAIYYEAYQERSPEAMFNLGFMHEFGAGVPKDLKLAKKFYDMAVYTQPSASLPVACATTWLYIHRWWDSMRPYMPSWLWSSVFALQPPHTNVMGSLLARLEGALPNAALDVGSVLWRWVVDLMGWGPLGDMVRQGDWYEGMFLLALLVLLVLVLRVRRDRAAARQRRLEAGQQLPQQPQQQLADMARRGAAAMAPEGQGGGIWGAATGSGVGSGRPGLQGASPTDRKVTATSGGLGTGGLDAAAREAVDGASGSAVDGKRPTASSSEPVAGAAAFLGSGGSASAGSAEESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIT0</accession>
    <name>A0ABQ5SIT0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021861">
        <property type="entry name" value="THO_THOC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265:SF0">
        <property type="entry name" value="HPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265">
        <property type="entry name" value="THO COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11957">
        <property type="entry name" value="efThoc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="706"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="497"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="737" mass="79382" checksum="B774629F1AA2AD64" modified="2025-10-08" version="1">MTILTEYEIEKSRIEDTILSALNASLLPAVAARKALDGAFPSAIFEHKDDGTLFVQGLDAAQRQNLEDALFELNLSLANQAVARGLPLMQYLHPDATHDGITTLPRFLQICLEFAQPLLTEPIKDTEKAGGRRRLLDYEGWARLFEGLMEVGTIADCSDLFRLMEGGSDVLATASNSTRAATHGGDGSLGRTGRDPNRDLGWAAVFCSDDFRREQGAKLALLRMSNMLFRRLSKAYDVRLCGRLLIFISRCYELNDKSGLNVVGALNGGLTLPVDEVEPGAVDSIGEQIDRRLYTCFWGLQAIFQNPTQVLQPAVYGKVMADMSLVLDEFAAIKNPVAATGPWVAARHTGNQNRPHLRGGGDMADGMEVDRSGEGCREEAACSRSQVSESGSGLGTRYLTSSKLFGLQVRDCTFRRHFLLQCLALLQYIEQPNKSDKSAIGQRPEGLEAMRAKLFSALSDSKEYGPDFAAAVRGVLEHEAHWVAWKHGTHPPPPGSAGPATTSPPASAAAGSAAGSGAASAAAGELCRDFTRPPAAPTREITSTSHGDTGGAGPPRRKRGAPSGRAGSGAASGGGRFAVSRGVLGLPDPLAGLKVSERRTRARNLMGEEEIVPMLPTPAELLVPILEDLDPDNDVEPQYRRVREAVFAWKRMRTLARNYLEFYKKGDDLEESVLEMHPELKTKIATRKAEREAELAAAAMAEAAEAGEVLPDAMTDGAANDPNKPEEGEAVASPRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIU2</accession>
    <name>A0ABQ5SIU2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014105</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19906">
        <property type="entry name" value="DSRM_ADAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044454">
        <property type="entry name" value="ADAD2_DSRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="207"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="5">
        <location>
            <begin position="568"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="502"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="640"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="787"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1067"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="481"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="659"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="680"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="802"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="848"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="870"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1071"/>
            <end position="1080"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1217" mass="119709" checksum="D24E19CB06BD3226" modified="2025-10-08" version="1">MSAGSPSLTGLSDGPNKKSRLLELIQLPESLDDAMLQRNAATPIQQCLPADEAQHQLGTLQLGSGDELASPVSTAAAPFVRADTAVLVQAPLATDTPMNAEDQTPDAVEVAATMALVSAAASAPTAGHVHAASAARGEKPLIPPAAQREGSLDPAPSDPAVCISYDCVADSSGLEGLGGDEGEGEVASRRTALAGSSGAGEGRAGGFEVFIGGLAPDARDEDVRAALGELAQGEVLSVRLQLTRGMSICKGYGFATFADEAAAQRAVSAAADGVQLCGRRVGLHASRRPFRAVLAAAQAAAAQLPLHLSPEAGASINGLLEILREHPDRRAAVAAALRVMQRGAKRTYPSSAALPLPCANVDNGGGSAAAAAAAAAATTAGSSQRAPAEAKRHATAATVAAASAAVPIKETVPGTPCPPSGSGGDGGQTTAAAGNTTAVAPAAASVPTSVAAPAAAAVEAPAVAGEDAKGTLVVKKETPVEASSAGRGSEAAGGSGAAKAAATAKKASGEKARGGGGAGPPGSGAAAPSTSGGAIGGMSQDLLAALTGYPKILNLLETLKDNARTAKTPVMILHEYAAKMTYEVTYHETSDGPAGPYTVESRLMSGGQKRTTVATASAKARTKKDAKQVSAAALVEKLLDGPRGLSPNDLLPVPKPLGAGAGSTRGGGGKAGSEKNRGAQGNGGGNGRKGPGGRSSGTQAGRFAPLPLPQQQQQQHNAPFYGRDGLNKVGASAPSLKMSPPPPPPSFHGPLMSSRGGGMLYSGGACNLGPIGRGPFSSSGGGGGYSSVQLGLTSGNPSLQQPGGGLGHHGTGVGGGSGASGPLGGLSEGRVGASREHRLGGPSYGDSRNGGGSRGSGGGGGSMVQVGLSSSGGAGGGGGTTTRYGGSYGREDAQGANIRMGLDLNDGSSSYDMAAGHKRNYSTAMQATYGAPAASQGLLPVQPQPAAGAVGSMLGNGVRSQDPFAVQVGGGSRGGPGGGSILGAGIGGSQVVGLQQHQQSVVLGQMQVQPGAFLQQQQQQQQQLAGQPAFGSSQQAQPLFAPTQQQQQLGIVTAAAAAGPPVQQAFSGYRQDQGPPQQQQHLSYGQQPPGGVALASAAAGGGGFGSGSYSGNQIAQLQPVPASQPLFGQQPAQQAMYTPNTYGMGSYTHSQAQQPQQQYASSGMYGNVSQATQGSGYAGGAILQQQQAQGASQSYGMSSTAGLQSYSGVRTGGGLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIV3</accession>
    <name>A0ABQ5SIV3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Fatty acid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_013956</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The histidine box domains are involved in binding the catalytic metal ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF31">
        <property type="entry name" value="DESATURASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="15">
        <location>
            <begin position="166"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI69364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="419" mass="47883" checksum="5128881957CE2B0A" modified="2025-10-08" version="1">MHTCSKLQARDNLRCTVRHSGHLMLLEKNLKCFGPGSCRLRRRASLCAKLQQSEAATENSHKELVVERVSNTGGSRKAFAKAFTSLLGLKLSKPEDLDPETLRDPPFAQYQVPGSDLVVQKLRPYFFGRTYTDTDVGYLAFFASMHLVALIGGPLTFSWDALEVCLAGYVLTGMLGISVSYHRQLSHKSFRCVKPLEYFLAYCGALAFEGDPIEWSKMHRWHHMHSDTPADRHSPRDGVWHSHMGWLFDESLNSTRRDANGNMKDSLSAPWFLKESPEFYSWLRATYMYHQIGQATFFYMLGGVPYLVWGFVIRVLLTMHMTWLVNSAVHIWGEQRFDTGGDNSRNNWLVGLLVFGDGHHNNHHAFEYSAAHGLEWWEIDMSYYFIRALELLGLAWDVRRPSPAQLVAKRIPNHNLPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIW0</accession>
    <name>A0ABQ5SIW0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_014609</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF379">
        <property type="entry name" value="CALPAIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Calpain catalytic" evidence="7">
        <location>
            <begin position="70"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="456" mass="51410" checksum="BB1AC14A398E41FA" modified="2025-10-08" version="1">MKPSKATLASAERGPGCTPSRWRCLGKAIFWPFILLLQALRIYLLGCILVYISRLGRGILCGMCVCCHCTFKDKSFPHSHISIGEWKGKTPAQIDSEVQWRRIADIVAASSRRGAKLFAGRIEPADICQGQLGDCWLMSALACLANQDGAVQQIFVTKEYNAYGKYKVRLYDAPKEAWVTVAVDDWIPCGDNGLPVFAKPNGDEAWVLLLEKAMAKFKGSYARLDGGSTMWALECLTGDFVFKFNMDSKTGKWKRFDIVHQAKPEGGGYNVMLRPSDDELDSDDMFNSMLFYNRKRSFIAASSGSGTDTQDINGIVQGHAYAICNVKRVDRFRLMQLRNPWGTFEWKGSWSDASSLWEENPKVKRALDFKPADDGTFWMEWKDFCEYYKCLDFCASTTGFEDISLDIHEERPYCGPVLGCLEGCFRYWVCCLGVRALFCSRKSRHFEQPTTGCCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ15</accession>
    <name>A0ABQ5SJ15_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014293</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02958">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049483">
        <property type="entry name" value="FAF1_2-like_UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF1">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21021">
        <property type="entry name" value="FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="3">
        <location>
            <begin position="444"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="379"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="527" mass="56715" checksum="73AA7E0E4DFEE95E" modified="2025-10-08" version="1">MADGGYVPDDLAVNRLQMVAHCDEAQARFLLEAARGNMDLALQMAIENSDAFGEHGGQAERVIPQHAGPQLYPQIPGGQPGVGGGTAFDGDLLPPRPRAPMISRGIAGSGGGAAGGAAATAGGAVAGTTLAAPLSLLTFILRVPLLALGFGFRMMRFTVINGVHAAAIVGDRVLPARVMRAARTVAATVAAGTGVGREELDPTGQAANFLAAFKAAYGDRHPRWQESGWRVACQQARREFKFLFVYLHSPEHEDTDLFCRTVLTCPDVVDYINANFVSWGGDVTTSDAFVLSQQTQMAVTRFPYVALLSMAPADNRVQLVATASGPAIEDPQSLLTLLRGAVANFGALLAAQRAEIEEREHARRLVEEQDAEYEASLAADRRREAERAEERRRQEEEDQRRAEEERQAREAADAEAARLADAAASVERRRQAARAALLPEPPAGSEGSAAIRLRLPDGTNVARCFPKRATLQAVFDFVDSLDATSYYRYHLAASYPRRVFLRAAHGPASLQELQLVPQAALFVQPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJI9</accession>
    <name>A0ABQ5SJI9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014969</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="132" mass="15659" checksum="5861BC2A13B2339F" modified="2025-10-08" version="1">MCMCAGSSKKYLPKEEVVEVPDFRYIPEPQLSELVAYMRQRREHLVYHNTTADVRAEIIRNVTEHTQPVDQYPRGRQFLNDEEYMSILRDLLNLSNILDDMEQTGIVSYTNRTQYYALYSKLYRMLIAKAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJK3</accession>
    <name>A0ABQ5SJK3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_014848</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF375">
        <property type="entry name" value="PEPTIDASE A1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045748563" description="Peptidase A1 domain-containing protein" evidence="12">
        <location>
            <begin position="26"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="13">
        <location>
            <begin position="72"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="434"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="439"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI70075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="514" mass="56385" checksum="EEBFA9049C7B1C2F" modified="2025-10-08" version="1">MLVVRFLGLTFALLFAVNRVSEANADDPAARQTVRQGYMVILRHRIQPGWRRQLLRNGPLPVFGSTRELGYFYSVLDLGTPTRSYSVIIDTGSTITYIPCKGCENCGKHQSERFDPDTSVTARRLLCGDPLCNCGSPSCMCVSNNQCYYKRAYAEGSVSEGYMLTDVLNFPDGQSPVNVVFGCVNGERGELYRQMADGLMGMGNNNNAFQSQLVANGVIDDVFSLCFGFPRNGVLLLGDVPLPEGTPPTVYTPLLHNTHLHFYNVRMEGIEVLGERLPLDAVIFERGYGTVLDSGTTFTYLPSVAFEAMSRAVGQFAEAKGLQRTSGADPQYNDICWSGAPDNFDDLDKVFPYAEFVFGGNVRLRLRPSRFLFLSRPAEYCLSIFDNGGSGTLIGTGTVQDILVTYDRRNNRVGFTDTSCVGLAEALAARANTTVPITPSSPPAFPPPPSPSQFPSPQPPVPHAKPPMMRKAPSPSSSSGHPHPHTHHKVHHHPSGAPLHKAHVLQRSHWLRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK09</accession>
    <name>A0ABQ5SK09_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cleavage/polyadenylation specificity factor A subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014434</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="59372" checksum="6C70736A138676F9" modified="2025-10-08" version="1" fragment="single">DQGLLAYQVAELGPDAEEKSVGGTIEAVVDCSLAVVVGAQGTDAGTDHLSHGGAPAGLLPPHQQLLLSRGQGDIILMRRRGDHVSYSNVVHPLRPRLPHTGVRPLILEAAFAEENGYITAVVWAMRSKAPHQAACCEVFVVVLGLRVEPEGQMGPGGEAASGDAGPMSLSLTTLDVRLLLRTKLPPYVTRVLPPEPQLHDSRSPPHSQPAAGDRSGGGQDRVLLVGFDPNTAGLEDEHEEGGAEATVGDAVNVAGGRGDDGIDDDDDMDPRTLEQAAARLAHITSAQQEGPQARAQWADIYKETGPDGVGAMGDEPAVDLAAWRLSNNSGGSGGVGVTGQADKPLTPFWRLCCGAHRLLTPEGPVQMFYHHQPQPQPLAKPLLLGVTDDVDCAVLQVDCWRRGEAKQPESGPAAGEEKEAEAGQLAAVQVRHLVSIPAIAYVAAGKTYKRHLLLSSSGTTTAAGKLGNSKTSLAAVLVESHRFMYLYGVTTTQQEYGMQQVVELGLEEGETVLGARFVEIEAMPAAEALSGISGVRCGPTAGAAAAAGGDSMVVVATQRRLLSYRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK80</accession>
    <name>A0ABQ5SK80_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014768</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF408">
        <property type="entry name" value="OS09G0560450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="29"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="31"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="82"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="133"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="189"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="249"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="300"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="354"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="440" mass="46297" checksum="954B2F74FA785794" modified="2025-10-08" version="1">MATDEHASEYTRRLTADRQRRDMGKALQRVLATFGTAADGRLGLGFPLQSQFYPRIVASLAGYAIKQVACGGAHTAVVTDDGALLTFGLNTWGQLGHSKADKFVAAPIEVGLPDPVSAVAAGGHHTLALTTAGEVWAWGMAGEGQLGSGDSSSGEGRQIEPRLVRALSGAGVVALAAGDHHSMALTSAGELFTWGRGDQGALGHGTVHGYAGVELTPRQVRSLSPSPGSPRMTSICAGPFSSGAIDSRGNAYVWGYGNSYQLGTGRAHNEMQPVKLDALPGLVRHLSLGQLHSLAVLGDGRVMAMGMDEHGSLGGGAEALQRRVVQRPLAVPGLPPAWLAVAGWKHSAAVGHDGRLFAWGWSGAAGAGVFQDYGGGQLGLGDDEDRWHPEQVLRLHTNRHRYYDLRASYIRPWRVLGLAAGRNHTAAIIETELDVRDLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLP1</accession>
    <name>A0ABQ5SLP1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histone deacetylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015928</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13035" checksum="5D6B4B2ED5D9D2F8" modified="2025-10-08" version="1" fragment="single">ILDRIQSHTWPLPLRIVTETGAEPPSTSSSTSPYLSSFGSDCGLQLLESHQITTTEELSLVHPASYIAAVRATCQKLREPTLVDDSTYLVPESYDDCCRAVHAARLRNPARFSPAPIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMF6</accession>
    <name>A0ABQ5SMF6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ANK_REP_REGION domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_016263</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="555"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="941"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="83"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="443"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="740"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="979"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="753"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="979"/>
            <end position="988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI71175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1109" mass="115777" checksum="D13314F84DD4D429" modified="2025-10-08" version="1">MGNEVSISQTDIQGSLVDLQARIRAGASVNKRMLFPNDTGPLPKGCKCTPLGYSILSGNLELLDTLIYAGVDVNKPVGLRDRFHFTPLQLAVAVGSGEAVRRLLERGADPNAALQSKRGTITLLQSSQHRHRPATAAIGSRPIGANTSSISPSASPLSLLVPEVLSSMRSGDTALHIAIDCAVEEHHHGVTSGSASGCRTKIVEALIAHPSTDLNRPNGRRRTPLYKACKRQLELVVTLLATHPRIDVNAGWPLFAAIKVQNLQLVRVLLQAGAGPTCGAYKIEGHTPLSYALRGRPSDSFYDRAGMVEMLVQAGSHVEPEMLSYAEERNWHAVLAALRFGAALGLSAEGPASGSGGRSGDGVVQPGPAMAEGHGTGALQLRASGTSRIQLSFLARFRHRLMRAHESYVSHKRSSHSRSWDDGVSGSIGGGAPAAAASTLATAARSGGGSGRLGNSVSAGGRGGGSSSRQRVSSTARIGTSVTPDGASARHRFGARSWTWRIRFPFPRAPSLQPQSQRLSEVVLKNPIVILFAPLLIPILIFEPIFLAFGEIFKVLAIVLVFLAVVVVPALLYGVYRLSRGLVRLLYNNRRLQNSSLVPFEPSSETSDAPAQSLPSFHQTTASISQLLVPQDSLSGAATAAAAGVDAVDRGSSGRQTVIENVNAVIVGLSRESNNRTGAAADPITNGSELLQPAKLKDSYFNLPASSSLPSSVPPPPPEALDGLAESTFRLRPQHLAQQLPGAGLGASPSGVSSSDTAAPPAAASTSAVGGVNFCSELTSSSSTGGSTVGAGGAAASGVTFSGVTSGSGSSAGGYTAVCSNHSSDSFVRDNAFSTVSSFLSGDIELAELLWRLGLEICVVLSVVTLTVIAVTYLDAFLEFHLARFLPTDSMLQICMRGRGTDRFRYPQGSLATCFSRKERTFLARLFFGTLVVRWISGAPAWVLTGLVAVVWLGCLLKALCDDYRLMCGLRVLEMTHASQGGSDSRSLGSEVAVGNGAQPESQPTAGPRNGGAPLSGVAGTHTDVVTGAEAAATAAPEGCTSTRNVTDGLDGLCCVCMSNRAVMGFVHRSVVHCCLCEECEGVLRGRHAIANCLICKHPASVVAKVIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMS5</accession>
    <name>A0ABQ5SMS5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="273" mass="27188" checksum="742F3F19D0A3C11D" modified="2025-10-08" version="1">MSYARFDGLETLDSRSRRTSYYDSARLWVRKPKNRNVLIGVAIASCLIVVIITASVCGSGKCGSGNSSPAPAQATDRSSSNAIALPPSPPPPSPPSPPSPPSPTPSPTPSPPSPPTPSPTPSPTPSPTSPSPTPPSPPSPPSPTPSPPSPPSPPSPTPTPPSPTPPTPTPPPTTTPPTTTPTTPSPPTPTPSPTTTPSPTTPSPTTPSPPSPPSPTPSPPSPPTPSPPSPPSPPSPPSPPTPSPPSPSPTPPSPPSPPTPTSPSPKGRHRRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMX7</accession>
    <name>A0ABQ5SMX7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase family A palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016402</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="DNA POLYMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133:SF27">
        <property type="entry name" value="DNA POLYMERASE NU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="3">
        <location>
            <begin position="1442"/>
            <end position="1671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="477"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1104"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1285"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="518"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1106"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1296"/>
            <end position="1311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1767" mass="190240" checksum="7F4880094F10B6F0" modified="2025-10-08" version="1">MSIAGATHQPSCSFRSVPLFCTPPVLVDSSYRPMRRGLPLLQNLAVPCQACLLGSTLGSPIRSRLGSARMLPRTYGGTYGSRSAPEGPTISAPRGVPYEQIDRAFVNARRNAEEAKRLSKNYGSISAAQLRRAQQAVLGPATATAAGVEPINTTTLKMLPSGDGDPGGGRLGGRSMQPSPPPPHPLPVQQPLHYQQLQHQLQQLQPPAQSQALATVAMAPEEQQHQQRYLLQPPALSRYVALQPPPPQALWQPPSPPPPQRVVQQPLDGRQLFSTLAPEPDGAPHDAYVATANLHDHIDCDDVRLAKSHQQQQQQLVQRQDQARGDELSEVLLSVLWDGSVRSPEQTVWAEASTSGGATATAVRGLLGGRRHKASARADAVALARRTYQPPQRDAALPYTSSRVTASSHVHANGNGTVYTVAEAVEVVEGFGSQDGVVRVTMESGDSGGLEGSMSYTAAAAAPQRSMPIMVHDPQPFKGSPEVGQEVQVQDHDAMAPPKAKKTRTRKRPTDIQLTTDETGKDVKTQAAVRDDGVNPTDAVGVPTTKRTRRKKPLATATGNGTASDSGDGGASRVPDDADAVPRAPPPSEEVKPYLQRGLRPLWPPVPVPGFDGYFEACEGYCTKQPLMYEEPQDVLIVNTPEVAKAVVDKLMTFASIPYPNNRINPTVPCRYFGCDTEVAFIDVTSETPIGHGAVLCLSIYGGDDLDFTDCECTAAASRAASYGSAAIVTADTTGGPPPPPPPPSVRKDRIWVDVWGEVELDESGRPIMDGRYVRLKPQHPILDEFKRFFEDDSVKKVWHNYGFDRHVLENMGICCQGFGGDTLHMARLADASRSGKRTYGLDNLTADKDIMRHFVPSKAEKAPRELTGSARRAARYGGPAATTALTLPPPSAPSPGDKRAAEVAKLVRAKVSMKDRFGLIKIRNGVPGKVPELPAMQLLHTLEHFRPSWIDYSALDAKATWQLREALEGLLRLERWELEPSLAQRLGYDTAADRGPFLHLWHFYCSYWLDFGELLTEMERRGMMVNRRHLAEAQRRAEADKEAAMKEFLEWADSKVPGARYMNAHSGLQIRMLLFPDFPGYVSKAAAAAAGKVEAAARRATRKAAGGGGGGGGGGGGTPLTPPVMAATTEAGGGMDAAASGEDAASASAPAKAAVVEKSDMLPGGCRIIKVPNPLYEEQLAQGKRVSRFMNLELYGIWGRGVPGRLQPEILTATNAAAVSIKVLRGLAGKPGAARKELAKMLEARAAAAADSRAAEEAARVAAAAARVTIVEDVLDEALQELTATEEKGTSAGAEGEEEAVEAGGDDGEEVAADSACLGPVPVVDDPMLMAALEKEAKDKGLGRLYAASGGGEEGLRACIAVEALCEISAIDKLLSAFIIPLQSDDTSTTQLLPASPGTDSSTRAVHRVHCSLNINTETGRLSARRPNLQNQPALEKDRYKVRRAFTADTESGNTLVVADYGQLELRILAHMTDCKSMKEAFELGGDFHSRTAYGMYDYIQDAVRNGECYLEWEGQGGPDKPLVKDKYAAERRKAKVLNFSIAYGKTAHGLAADFKTTTEEAQETVNKWYSDRWEVRKWQEDTRKGAREEGKVRTLLGRTRPLPNINSPDFKLSGHSNRAAINTPIQGSAADVAAAAMVAIHKSEALRATGFKLLMQIHDEVILEGPRDRSEDAKRIVMALMANPWAELSRHWNAPGAVGLMQGLDWHRPLPLVPGGHLAGVWRHGGEVVAGEGRKTGDCKGGDIMPLLVELATDCNVADTWYEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN45</accession>
    <name>A0ABQ5SN45_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016525</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="49"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="377"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="380"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="404"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="421"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="443"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="508" mass="53742" checksum="D54D095446DA648B" modified="2025-10-08" version="1">MPPNPVSLQGMVLHRRTASTRFAPSSVKQPHIGPSGSRTIVRVHASLLNPHSILGVSPTADVKEVKRAYRKLALQFHPDVCKSEDGHERFLALTQAYEMLLGRAEGKTDPNHASSSGWDFHDWYWNFRMQRSWEKQQRQAKAAAAAAAGADMSDGRPGDTEPGCGRRAPPHGHAGYKQPEARANLRSQLAGLRHRAAVRANRPSASAASSTVSSPSPASSSASPPASCSAASGFSMDAEDAGPWTAVSCAQTRNHASRMEPISGAGAGKGEAAVTFSEEATTSESKWSWSWSWDEILKSEDEQLNVAGVSAARADASSSSNEGQSAQSFPSSCGDPATELHHQYTHVYKAAAAEGMVVHDGHLGTIVGAANHAVPFPSGASVSSTASKQQYQRSQPHQTHDFRSSSQQDQSLLSGLNSAEPEPEPETETEPEPFSNPFDKIHFQQQHSTRRSFSADNASRESVSHQLAGLRRKAAMKATHYSTGTGTAAGAGVGVGAGAGGAGDVDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNQ5</accession>
    <name>A0ABQ5SNQ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dynein regulatory complex protein 1/2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_016374</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DRC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039505">
        <property type="entry name" value="DRC1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039750">
        <property type="entry name" value="DRC1/DRC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029440">
        <property type="entry name" value="DRC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625:SF1">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625">
        <property type="entry name" value="NYD-SP28 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14772">
        <property type="entry name" value="NYD-SP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14775">
        <property type="entry name" value="NYD-SP28_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein regulatory complex protein 1/2 N-terminal" evidence="5">
        <location>
            <begin position="75"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein regulatory complex protein 1 C-terminal" evidence="6">
        <location>
            <begin position="600"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="413"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="203"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="423"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="444"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="675" mass="78004" checksum="9FAEEEDB6E8316DA" modified="2025-10-08" version="1">MDDIQFQSEREARILARRKRIQERLAALREGDHGGGKEGEKKEEIGKGKQQIIESKRRLMRVKFRTDQDVTSVRVAGDDRENQHRIQEEQTRQDLRAKLLAEAEQSARQNAAVAMRWADLFSIEVPQDLYTEIEAQRQACERIISSKDGLIGEVRAELKKKDDEFVKTLKRQAEDIDTLLQYMSRQFVEMQNAFKEELDEIENAFLQERSDLLESNRREMQELFDRRSRLEQDFMDRYLAAVENYQQQLEGHRQMDAEEYHILKIKLETDIQNLEQHLEAMRATYQLNTEKLEYNYRVLKEREKENSTTIESQKKKLSRQRDILSSLKQRYAETDRRYRDENMRLTDEYKRITEQFKDLQSKFRHFELVDTKKYKEVWGMKESDVAALVRQLLQADKVLHEQQLGWDWRPPDDAVFTPVHTSGPGGASGAAGGEGGEDEQDEDAAARAREQEVSERLRDQRNWGALGLLCDEAGFLLDSKAKHMIDRLPKDEQGLVKAEAILRALGIADGASFDQLIDALSADSTVEMRAKGMGVPAARIVDEKGERGPAVLVHPDDAVRRLKAFMEVYGTGPSRAGGGAISGPLRVQGLLRRAAEREQEFWARMTHVISDKGVRVWKALEKQLERYLVLLQERSGSLRDVESLQHQNNELRALLNQYLSSRINEELQIPPTQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPB6</accession>
    <name>A0ABQ5SPB6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016715</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="655" mass="70117" checksum="AD4F811BB5807719" modified="2025-10-08" version="1">MELSGHWARCLCLRHGGRALRLACAAQRPLHSTPEPAPIASHKEQLQRSLNLGPAVKQAPDFEYVAVAFRSQPLSKHKKGPQRAGGSTVKHKGGFSKIFGETDGGHGYRTLEVVSGDRRLLFEAAGCVSDANPASPEVVPDSSVVLNNAGKRHAIIAAGSERANLPIPHKRRQVKLKSQQRQSHMKQAGAQVCDCPNVAILTVPGFATWLGASGSSDSTMCESLPPLQDTSALTAHSEAARDAPGGPCQNTSPGAPMSMCAHGHQIGTTATPRGREEPLQVWSDPLKPEQEPLLQPHRRLHIQPSRQLHTANRRVRHISVVPGASLAAAERLAAAAELLKLPPTELRRYRGFCSELRALESPPSALTALCGVLVQVLGLSTQQLCAVLLRQPHVLRVPAGVLARRGAALALELQLPPPPGPGGGGPLAAIVVAAPDVLLRRGDFAGTAAALSAALALPPTEALELLRREPQLLRHPPGFYALGMAQLREQLGLQTPAAAALAVAEPKLLAVSPSVLRANSAMLRSRLQLHPEQLAEVAARAPELLVRSPGALRAVVQRLTAVLSRSAAWREALLRLLGSPRNLAVALSFGSDRYDRLEYLSATGRDCIMSFKEGLSLGDDEFKEIFPEYVYNAWRQAVPHKHAQLGRQMRSHMGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPD7</accession>
    <name>A0ABQ5SPD7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="136" mass="14410" checksum="76FB80DD38CA2C18" modified="2025-10-08" version="1">MEASDGEPPAKRVRAHAPQAAAAAVGTGPSSASRPRQCDKRPSSGGRGNARGGRRGHQPGRGGGIKPQSLTVDQERELTRFYNCYGRQLTRGDLRDLRQEAGGNVCFCCRAAGHSYDECTLRPPEARAAVAQGNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ14</accession>
    <name>A0ABQ5SQ14_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ran guanine nucleotide release factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016694</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MOG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007681">
        <property type="entry name" value="Mog1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15837">
        <property type="entry name" value="RAN GUANINE NUCLEOTIDE RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15837:SF0">
        <property type="entry name" value="RAN GUANINE NUCLEOTIDE RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04603">
        <property type="entry name" value="Mog1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20141" checksum="A3A6063B8B38E582" modified="2025-10-08" version="1">MMTVPNPGDATQRGLWGGAVTCVVPTRMVDVSDIRQVPDNQEVFMDAEANQTIIFEIVEHDETVPDEECGRYVFEDLASENEAESCGLESLTTLGPANAPYVPVAAYMCLVRGRQLIRQGHEFKDTDLQLAVLRFPWVQSDLIISVCTPVAAPELCVQASTIMEEVLSSLRILLWELFGGGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ40</accession>
    <name>A0ABQ5SQ40_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016709</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="780"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="963"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1019"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1222"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="589"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="789"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="813"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="835"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="913"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="981"/>
            <end position="996"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1303" mass="136108" checksum="682DC21A49965667" modified="2025-10-08" version="1">MDFAENWCSLLELFVSNAGLKLRMFMVDTVLIKSGRVLGHFYTSKDGYICRFSAHEITRQAIYQRLASLHCLDPQLNPFGYIGVAHYATGVSRLLKRAELQDLMTTICGPYEFSDSSPIATQHEQLFCVQAYIVPRHDMRYIASYDVQEGPLCTVLARRFSSRYTNAMDDPASVLVETASSDPDEPARTSSILAGTTSINRPLSSVMALSMSMSLDDPLTMPSSLTADELLVESQLVAMGLLEPRRQKEREPAAAGAEATGGGAEEAGVGSGVGGGDDAGATSPSGSPPDGITSSLPAPRRVKDDVARLLQAASSFVERAHGQRIVGLVAEFITAGAPTPGLPSGGGLVLMAVHAVQLDPRASRGRLGTFTERWTDYLEGLAPPPAPQAPGRRNQSPSILRAGDSSLVMTERSSGFLVRTSPGSSMGSMGGAGGSVLPVYGGAFNPQRQVLGVAPTGVSGGAGSGNVISPRVHGGAGSGASSPAAGSLTRIYSASPSAAHHRSMTALSDVGRPGSAPAAVVKVGRHLPNAAHSSALNGHYTLRNAMSAARPGGGGNPTANPGAPDSYSLWLSREGLPTDSMSAKLALEVESLRERLQRQTDIAVRAEAALQQLAVSSRKETMELRQQIEELRIEVGRLGEAKRNLATEYDRLRSERLGLQAGRSELSGEVERLRTELAGERETLAKAVRDATAREEQLEGQLRAAREEREEALKQLGGLRRRVEEESEVVEALRGQLYDYKQLVAQLQAQVRRGRNNKQLLQSLQGLPGAIASAANLNAAGGSGGAAAGDGTAAARGGRNTKDGEESDADTDAGGGASGGMDGTGSQQGGQKPLPGGGGGATGRQGVVGAGYGGRRPSNTINSAFHHGGSSPPHHRQTFTSSSPHGPDHGASSPRRGASPFRGSVGSGPANRSVTVVPPTSNPTPAAATTAASSPLMDVSGAAGPTNLLSEIRRKRVAAGGGPPLLALERADSSREGPSVPAANSTGGASAGGSATAAAPNGNGGAFGAAVGPAAAAAAGATSGGGTEGGTNAARPIAPPSPTGSISGTTDGPVLVSSASLNGKAMVVGLGARKPNYDMVQVYDLFLMSWIRESLTPRMLECHGIALQEVAAEETALAEIFNFYGQLGQVVWLDSRLTMNEKQFVKLALETGVPESSTNDMREVFQKLSDKTEFASEAASNHPHPYINFEQFPEAVLRLAALRYEWRPPENLEDAEEELYFASPPFMPPNPRESQLGDGDEEPPPRPPETVFVQIVKAYLKDDLLIRARRFKTAGSTQAKFRSQLRTAPQGLWLSSKSLTAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ97</accession>
    <name>A0ABQ5SQ97_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_017152</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M43B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000123">
        <property type="protein sequence ID" value="GLI71801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47466">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47466:SF1">
        <property type="entry name" value="METALLOPROTEASE MEP1 (AFU_ORTHOLOGUE AFUA_1G07730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14295">
        <property type="entry name" value="PAN_4"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046378408" description="Apple domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="4">
        <location>
            <begin position="137"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="4">
        <location>
            <begin position="383"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="216"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="464"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="622" mass="67465" checksum="AA6D302C65C0288F" modified="2025-10-08" version="1">MLMSFKMLAIFVFVLPFAAGVSGTARRDLSSSTGYTTAVQQAVTIPPVAPALPWGCYAKIDFLGKDIRIINQTVDYLACRQECYNEPMCTFSVRTLDRKCYLKYSFLNGTAGSNNYSPNYVDVLCFVRPNYGGQYQCAADYDVKGDHVGDIAKACPSFATNISLQSCSNACSQDSRCEFFVHTDGNYCYLKLNPLTGVCGPTNTSTYVDKVCFRASGPPSPPPTSPPPPSPPPPSPKPPSPPPPSPPPPSPKPPSPPPPSPKPPSPPPPQLPLPRPQPPPKPLLASPPPPSPPVAPALPWGCYAKIDFLGKDIRIINQTVDYLACRQECYNEPMCTFSVRTLDRKCYLKYSFLNGTAGSNNYSPNYVDVLCFVRPNYGGQYQCAADYDVKGDHVGDIAKACPSFATNISLQSCSNACSQDSRCEFFVHTDGNYCYLKLNPLTGVCGPTNTSTYVDKVCFRASGPPSPPPPSPPPPSPSLPSPDAGSFCLRKAYNRKEGYYISPPCKSGYDEVYGMCIPKCADGYSLSDLFFCFGEVPSSYPVMCNSAVWATSQAGCDKINKVISELRKKYGYSSDECAASIYGNCNSWSTDQIIAFCGHYMLGFYEVYICDSVPLPAISVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQD1</accession>
    <name>A0ABQ5SQD1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KIF-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_016475</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KIF-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022083">
        <property type="entry name" value="KBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46321:SF1">
        <property type="entry name" value="KIF-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46321">
        <property type="entry name" value="KIF1-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12309">
        <property type="entry name" value="KBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI71316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="451" mass="48919" checksum="FDAB25E5F8189434" modified="2025-10-08" version="1" fragment="single">AVAAAAASVADASEPHTAARDSLAAAVEAEKGLPLAEEAGAASSPPLAEQLPYNDTEAVQSQYTKTLYYLAQVYGHMKSSDRSALYCAATLYRQLASGKYDLDDWVNNCLQLGGYYAKVHAYGLAMYCHAAAQAVVDRDTAAGAVLGDDISANLQLATARVYNMRLAASHSKYVEGRELEMHYPDVSYFPDYLRFSGLSIPSPDFFPWGLEALAADFAAARELLNTGMPYFKAALSYYQLDGWVTDHVGIVMDMSNMYRCLAGFEADPARAAAMHRCRAQLLEPLSGALNPDHYPGLSRSIDLELAHVYREISDVRELQGGAAAKVAAAAAASAKYYQRFLRSFASSETGAMPDRVDQDNEPYFLMASFGLGRMLHRMRGGAGQGGGQANAEGSYESLVIAAHRHLQWTSEYVKRHGLEGWTQEAQAAEELSELLVEQMRLRAISAASRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMH7</accession>
    <name>A0ABQ5RMH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000344</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045551306" description="Protein kinase domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="919"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="744"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="837"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1037"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="745"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="785"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="841"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1061"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1085"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1128"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="946"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1337" mass="140652" checksum="D7D9C6BAA6D14B20" modified="2025-10-08" version="1">MSWYCLICPSTQLLIYACLISFVCSSHQEQQPSPANGYCSVGPPGSIESLSSLSPQQLAEGTLASSILSSWDFGVPLGSIKAASGVNVTLSCIVALNVSVSRPTGSNVFLQPMALDFSNATGLKMEQVLLRTNCSSVLEYQTYFCSSYSVAGSITLLQGTVHFTAWRDGFTTLRNVTLTCDNSEAAPQPPCWLTAVQDEGQLLDAFVSYSDIVSANVTVVIAANISISTSFWPKVPVRVSNNVQLVSSSRLYRPVLDFRLLVSLWEVLPTITVSLINITCVNLAPTYISPCFPASLFALMAHRLWAFRRTDRQLRIIDSTLVVSPEELQYMKYWLKFLISPVPEDKDKVSWMNLTQLTVNTVNDSGVYYDSAVTYTAIYKNVLLTDSAGGTYSLLPFDTGLQQILAEDVPLSAIRQVVNVSDMLIALSPNITQPDNEGRRWLLLVANFSLVDGNRSATDAGATVPIQLPGRMVVGVGGGLRAVRLDFGRQQNVLGVAAGSTLLLQSLILGGMPSRSADYDPQAPLEVLVSPLWGLSLVDGASGVTLENCTVLVSAVELQVLQASLLPPGMKVQAQAFDESLTAAVRAFFTASTVASYHSSYLRLASGATQRYSFTGITFRTPVVDDGEGGIPNIVAPSPGDEGSVLERWKVGVIVGCVVGGAVALAAAAGLLLIVRRRRAARSQGAYEKYLRIGGVGLCHGAPGNVGGGGGSLDLVHSSHELTNSSQVLQPDARRSDLEVARLKATGSGTTSASASGGIGATGRSASITGVTSQDASSGAVLPSGVGGSSASGAGQGGSGCIPGTAAGSGASGGLQLPVVPSLTVGNGVLVAGARAAEGDGSNQTTDSFERPPSNTVLGQHGPACRHVQRVMSIAESSSVPSSITNQSKSVLATGGASALEQMHLTIQTLGRDLNDRQLEVHGLLGRGAHGTVYRGTWRGLPVAVKSMVFSNDSSARQQSRVLMEAAISANLAHTNIVTTYSYELREVEHELASISQELARQGGGWRLLIIQEYCDAGPLRRLIDCGFFLTPPKPSSVRTKIKLPNPVGIMRKDSPSGEGSTSSATRVDATTAAATASCSRGSSGSSGGPNGNGGSRSSTGKEAEMAPAAAVMSRSGPGVGTLGQTGPSTSPQPVVQKQQSQQQRPSLDVIPSDVPGRPTTSLQAARRYVEAALMIARGLQHIHDKNIVHGDLNPNNVLLMHAPGTPLEFCLKVADFGLSVRMSEGASHMSNLFQGSPYYCAPEVVLSGKVGKSADIYSLGIMLWELQNGTRPLWRSGVRLRSYPSLNTAELDFGSEIPPRFVRLARNCFHGSKDARPSISAVVETLVGIKAELDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN32</accession>
    <name>A0ABQ5RN32_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="98"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="371" mass="42364" checksum="B2C6859261F29C28" modified="2025-10-08" version="1" fragment="single">MQKTDSRAPGSYVATAGTIDRDDAPPGYPESGMSPPGPMSASLQQQWTSWSAPGAAAGAPGGLMHQAAIPEHRVRKFHRLLQEPVVDLDALRELSWNGVPLALRPQVWRLLCGYLPPARSRQAQTLERRRREYADMVPEYYDIPHEERSEDEIAAYRQVLVDVPRTAPSVPFFHEPIIQESLQRLLYIWGIRHPASGYVQGMNDLVTPFLAVFLSEHMQGPMESWSADTLNQTIMLAVEADCYWCLCKLVEGIQDHYTYAQPGIQRAVFRIKELVRRCESDVSDHLEAEAVDFIQFALRWVNCLLVRELPFCLAIRLWDTYLAEGTGFSEFLVYLSAAFLISWKERLRLLEFQDLILFLQRLPTSEWTEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN89</accession>
    <name>A0ABQ5RN89_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cleavage and polyadenylation specificity factor subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cleavage and polyadenylation specificity factor 100 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000655</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16293">
        <property type="entry name" value="CPSF2-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027075">
        <property type="entry name" value="CPSF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035639">
        <property type="entry name" value="CPSF2_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922:SF1">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Beta-Casp" evidence="3">
        <location>
            <begin position="276"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="623"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="641"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="650" mass="67954" checksum="1D9C62F89A18D6F7" modified="2025-10-08" version="1" fragment="single">METSVRFTPLSGVDAESPLCYLLEIDSFTILLDCGWDENFDIAALEPIKRVIPRVNAVLISHPDLAHLGALPYLVGKCGLRAPIYSTKPVRRMGEMFMFESYLAKQAISDFETFDLDDVDAAFRLNVRWRELQYSQRHQLVPPPPPTPPDGDNDDDAAGSSGSAVAAVAAGGGIAIVAHPAGRYPGGSVWRISLGCGEEVVYAVDYNHRKERLLNRTHFDELLAAVQPALLVTDAFNALTENTDRHRRDEEFLGAITATVEAEGSVLIPTDAAGRVLELALLLDEHFSKARIAAAPVLLSATIKTVLEFARTQLEYLGNELVQAFSLKRAVPFSFRKLSVITRLEELGAFPGPKVVLATMPSLEGGPARELLVQWAALPRNTILFTERAPAGTLAAALQTHDPSAATGPLRLQLRMGRRVPLEGEELADWQAAKVAAAQELAEHGGSVKDSLPLLSPSSHLTPASRLAAAVRLANAGSAAAAAAAAANGGNGGGSAAVLSLLTSPSVGRGGGGGPTDQPLRASTGSISRLVRDSTTGAIVQTLTASTPGGPGAGGGVRKHHGGGGGWDAGLLMDGFEPPADAAFPMFPDEDEPLYCEWDEYGAVLGKDEFRVPTLTDGTGAAAGRFGAGGGDGMDVDVGDSTGGGGRGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNJ7</accession>
    <name>A0ABQ5RNJ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_000325</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NIPA (TC 2.A.7) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF9">
        <property type="entry name" value="MAGNESIUM TRANSPORTER NIPA8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="527"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="575"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="627"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI58597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="636" mass="68486" checksum="DD39BBFEBF09296B" modified="2025-10-08" version="1">MSGSSFPPPPLGLEEKPRDLWYVGAIINVVGSIAINLGTNLMKLGHNKRAKVDCAEAQKPPVHKFKEWCIGMSFFSVGNILNFVSFGFAAQSLLAALGSVQFVSNVIFAYFVLHEPINRMILIATACIVGGCILLVVFGNQTSATYTVKQLTELYTKPAYVVYLCLMGVGVVGGYILYLHGQKVVSKTGPRGIWYALLPVAYSVFSALIGTQSVLFSKSMSVILRLTFTGDNQLGNWYTWLALPLFLMTAVFWITRLNKGLRMFSAMVIVPVMQISWTLFSIVSGMLYFQEYKGFTPLKSVMFPIGVLVVFVGVFLLTQSASNKPASYQKMEEEAVAKAAKEDSAKKAFGSHDTTDFHLPVRAASIDSTRSESTVAVVDQGDAARLRGDSLDDGQHQLSRGPSGLVATGGDAPQVLQHAGPDALEDAALKAPDSQSMMGKVKGHLRTVQDRLNKDLLGVEPRAGLRLAMGLGDSGMPAISLFAMPTILNYTIADNESGRYSEVVITSPKEGEFATTYLSQTVEVMGKPLNSSTQPGDGKSAPEDAHQPAEGGSPVAARPPTAPSSPHLGQSGRRSLIDRAERAAKTGIEAIKRAPSNLQRAVREKQGYGSLNDDEETGLLEMTEPSNGPPAPTKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP47</accession>
    <name>A0ABQ5RP47_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="224" mass="24166" checksum="1621E17315D89122" modified="2025-10-08" version="1" fragment="single">PPPPPKPSPPSQPPPMAVCKTDDTQCRQCDTSAMFTSRDTCKYCYTQSVSKGQNYWYCFNCAERNSNATMQALCQIECVLSTAAGANVNACGSCSDASIVGSKADMARACYNCSRLVSNAWNCYHCLDLTKSLGSSADAASRACMTCVTKADDVWGCHNCIDVTKGLRDASDARDACTQCVMSGKLTSWECGTCAAKANPVDRANCFKSREKGMRRRLTENGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPN2</accession>
    <name>A0ABQ5RPN2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001329</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="4"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="283"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="607"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="889"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="1225"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="228"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="509"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="852"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1131"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1498"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2056"/>
            <end position="2087"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2162"/>
            <end position="2235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="523"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="550"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="570"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="855"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1131"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1152"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1502"/>
            <end position="1511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1533"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2056"/>
            <end position="2068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="2078"/>
            <end position="2087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="2164"/>
            <end position="2173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2189"/>
            <end position="2200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2291" mass="242612" checksum="EF0DEB60580AF25B" modified="2025-10-08" version="1">MSDDKDVRELAEAKKLPLPERIAHSNWKARVAAYEDISASCREIYDDADPRLSEFAPLFPKAVTESNAAAIDKALDALNAFLAKATEQHASRICEKTCGNIVAKCLSARTSTLTRVLDTTLLFIELEQADKVMEALVSGLSNKNPKVVVAALELMYHGLNLFGHRVVPPQPIMKALPSLFDSKDGKAREKVKDIVVELARWLGPDAVRAMLFDKMRDVMRDDINRHMEGLELSRKPPRLTRKQQAKAADPREQAALGNGATSNGGAAAAAAEDEPPPEADPYDFVDPKKILPELKSDNFWDKLEEKKWTERRDAVLLVKGLADTPRIASGDYGDLMRELRKLISKDSNVVVVAESINCCGLLAKGLRKEYAPWARNLAAPLLEKFKEKNSNVGNAVTGALTFMHRYCWSLTEVIEDFTEAMGHANPKVKEDTYKWLAGAIDQEPKANLKQLVAPLMGAAAKGAEEALPALREAAMGFMAAFTVRFGNTAILEKFGAKLDDARKKKLQDMVAEAMGSSSTTSKTAHPAATTQTPTPAAVPAAKTPGPALNLKSSRLGSSARPSGAVKPAAGGSGSGTGGSSAPAGAGGGRKIAGGGDGDDDSSLAAGTLSRDQAVAALTDLLGEATVKELQDDQWKVRLDAMERLVAFVGESGVAASNGSTLIQAVAHVPGWGEKNFQVMAKVLEALRVSAAGCPTFSKRDAFVCIGGIIDKVADLKLKQPSFDTLTSFAEVLGPQYVVTQLHKKSASHKNPKVQSESINWIARAILEFGLAGMDVRALLDWAKEDLGSANAGVRNSAIQLLGVMYRFLGPALGDMIRADVKPALMTAIDGEFAKNSDLPKPEPSRVSRVAVSARGGAGGAKGGTKGGTGGVAGAASSGSAAGGGFDPDDLLPRTDISGQITLELITQLGSSNWKERKAGLDAIEMILTQAGNRIQPQTGDLLPELKKRMADSNKNLTTQALTVLGRIAKAMGRAIDRQGRPLLASAIKNITDQKQTVRSAVTEMLDAWVGVTSASCVMPDVMDFYISSKITADGKAETLKWMTSLVSGGKVSDCVSDTLRAAAMGSADKAAEVRDAASKLMTILVETTGAAELGALVQSLDAASRKPAIEAISKVTGGPVPLAAATFSAVPTMSSAKPSATSATRSSKDLLRSSTSSISRPGTASSTGSRPGTAKASGVGKSTLGSSIGALAAADAGPLLMPDNRKEDRAKKGRFRPAKLQIMPDEAQTLEAEFSPMMNPALRAAAFNKDFKKHCEAADMVTKALPAVYDEVIAVVDLLFRWSTLRILESNTASLVKVLEMLKLLLDLMVERGYRLSEYEAKLILPALVEKSGHNQDKLKAEHRELLKRFAQVHPPIKVVAYVKDGLESKNSKTRVVCLDEIAAMVERTGPVVYRNSSGASTRPASGSAPNDSLMAAVARLVAERDTAVRAACLGVMEVLYCIEGPAVWDYVGRLTDQQKSLIEERIKAVANRLARAGTQPGFKAAEYGLQVQQQDDPMVVSPPATNNNTPRRPPSPMRRSGIGLGVAPSNNGGVGSPVGRGGLMDNLPARTAAASTAVPVAAAPARSESPPPAPKVPDAVSYDVRRMNSPLMGRFGSFNLTSAPMNGGDSTVLTSLEGPDEEGTLRNWVIMHDRLQGEDWEGATQAMKLLCYSYMELDKHNPHVVALLMDPRNADELVHLLSQRIEQSLSDAAASTMCLPGGPTYNARACKYSVNSLMNLCNVTSLTASLTDSALRRLCSVLIACLIDGRLRQVPDGDGLLKAVNMLIMKLLEHANRAGLFGGFIHCLRAPNPRIYEMVHPATAGPDGSAAEMLLRWNDMVVKCLIKMTKQLGNLMPNLNVGDVLVHIHRYMQDLGTEEVRRRSSIEDKPLRMVKTMLHELCKHRGYEIYKDVEAMPDVTESMEEMVMLPYIRLNLETLQRAGANLPGAAAAASLMAAAAAPRPAVAPNADVASTSASLSATVSRTRSIPPAIPAATAGAPGVISKPVGAPATVEPAPKQPQQPQVLAPLSTNAMPPLNASAVAQKLSSSASVPVAPPVAAPVPGLPAVAPIAAPAPSSSAATAPGSHVSPLSLEPSSREFKRPAMDEGEARGILATIFKRIGDKAQSQQALVDLHHFIEANPSNDVVNQMLNQVSPYFRQFLQRGLNKVRLQLQHAAAAAATTQEQPSGSPGNAAGKISDGGGGTSGSSWGTPDGDSSASLPSRMTIAEKLAASEARPHSPASPGRSRGVGNLDELRNRMNSLSTNLHDLPVRNSASISVPGGVAQDLLDLQERMKKIQSISAAAGPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ99</accession>
    <name>A0ABQ5RQ99_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001677</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF19">
        <property type="entry name" value="KATANIN P60 ATPASE-CONTAINING SUBUNIT A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="385"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="256"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="579"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="269"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="683" mass="69774" checksum="51C6AB0508F67813" modified="2025-10-08" version="1">MEDQHLQRAIYASIQTARSQGYNLGSGRGTVQTIRPSFQPSRGGSGGGGGHLGSHGTSTHRSPAAGATHGGNSHRNQAHRPGPSPQQPRQPHPRYAGNSLTGVGASASVAASSANPLRTSFGRIDALRRDGIVPASASPIFPQSNSHAQSHSRNHVHEADSGIQSPGSAPPRALPTATPPRAATTPTRSRNQAPKQPPSSQRHGFQSTLIGPHRSANHVLHAIAAVTAEAHAALDAAAAVGASPITVLPLSPSRATAAQVAQPPPLSPTGGGGGATTASTRGGGGGGGGGGGGGGGGVTGLGPVGGGGPPPRALISVAAPGGAVMMLPEGVDPVLAQAVLRDAVTWDTGVTFDDIAGCDRAKQLLHEAVALPLIIPEFFTGIREPWRGVLLHGPPGNGKTMLAKALAGMVGGAFFAVSPASLTSKWRGESEKLLAALFAVAQAHAPAIIFIDEVDALCGARGVEGEHEASRRFKAELLQQMDGLASGRGVMVLAGTNCPWDLDPALRRRLEKRIHIGLPDAAQRLALLQLHLRGVALAPDADLPALAASCEGFSGADIRLLCRDAAMAPLRRQIASLAVGNSGPTLPPPQHHHQRQTQEQQSPPAGHPQLSSPSPEALQVEVLQPLVLCSAADIRRLADSGKLARGAVVSAADLEAARGSVRPSVTADQAARYVQWDLEFAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQN2</accession>
    <name>A0ABQ5RQN2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001803</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323:SF1">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="711"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="746"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="791"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="832"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="540"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="619"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="943"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="345"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="540"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="567"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="950"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1098"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1098" mass="113845" checksum="347F5482723D0701" modified="2025-10-08" version="1" fragment="single">MADGEGNKPALSLLEEQEQHKGPSDAPEQTPAPYVQPYRPSPAIPPVAQQSNLSATGGAEGSIRSRLSNASYSYARGPGRLNSIISPQRSGSAGGADTSVNILAGAVGDVLPSGTLNNTHLAHLLAYTQQQLIQLQQIQTLLMQHLQSQGPSAGAVSPAASGAAHKALPSAADTAIAAGTLGTTNQAIYAPTAGPLTQIPPLKKPFSTAAGPTPAGAALADPALRPAVFNTMGPLAPESYPIPGSGAAAEQLSLVPSQSSSSLATTWPPPQPPSHVWTPLKEQSSVGSIARREGPLPTYPGGAAATAGSWLPVHHQESQVRQMHGQGTLLRVAVAQSMDPATAEQQQQQMQQVRLSQQAQMPPPQPPSSQQQQQQTPQQHVPTAHSAQLAVAPEPDGSMGGAPPTPSLFSAGLAYVGSDAPNAPSALAAAGGTADTFTPSSSILAHTIATSIHYVAGGSRGGDSYVTTAATATAPPQTNPPTGLQGGTRESQQSIALASPRLAQREGAQPSADGLSGGIPLAGEEMQPLAVMSLSVGLLPPSPPSPEPPSLVQRPALTPAAVPPPALSAMPSSEAPLVHPGSGASMGVQLPLPLPAAGGPLFPILPEGKFFTAGKTEEPMARAEDAAARGEPWASTGEAAAAAAAEQPRGKGVLDRNRDESVEFEGPELDAQLDANRKDLLVFPEEDEDGEDSEGVEIKKTPWYKRTDLRWFAGSAFAATVLAVTGICMQLGKFNQAALKAEIFRWLYLVAGYVPIYWGIYFLISRLFMLIEWVYFRENLTYLKNIKETSASLISMLLELLWFMACFVWLWCEKDRCSSSLYHDARDATWRVVLCIMLFTLANLIKVVTAKIFSMHFYRTAHFKKLKDSLEKEYYLQLLSVPRSRILAQITETQAEAEAAREGGARYQIKTVMERRSLFHTVIPLQRLGRITALVHAISGGGVNASESSKGGGAGDRAGGGASSSIHGNGGVGGGGGVVEGPASAGHGLARSSKAPVQRGTWAGFWQCGARGGTGGAESEGELMHQRARWLGRSQLSAVGGGDGGGIPSNRSVNELTEASTVSAAQGRGGGGAGGGGCGVLGGEVGGSGNTTMDVNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQX6</accession>
    <name>A0ABQ5RQX6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001783</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11189" checksum="7F40B2259BADE0BE" modified="2025-10-08" version="1">MAFPCNQFGGQEPGTNAEIKKFATDRGFSGSGALLMDKVDVNGPGTAAVYQYLKVASGDTSDIGWNFGKFLVRPDGTVFGRYSPRTSPLELEQYVVEMLNNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRR5</accession>
    <name>A0ABQ5RRR5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="128" mass="13591" checksum="1C6E3BB854D6EE36" modified="2025-10-08" version="1" fragment="single">ANYTGFSDLRLNNNLLAGTLEPAWAPLIWSSEAVRMGVQASPNGGWVGSFPSTWYSYTLNGSYVGSRLKELYISTATCTCGGPPPFPRGSWMYNKQAAKLLTVGTSGSFNDTSCSSSVCVLPPSPLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRV0</accession>
    <name>A0ABQ5RRV0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar FliJ protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029375">
        <property type="entry name" value="CFAP141"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15104">
        <property type="entry name" value="CFAP141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12659" checksum="7CF250AC772F7CAF" modified="2025-10-08" version="1">MSYVPKNVRDTARKNDLYAKLEREQAQETHHSVVAHWAERDRRREPVNTLRGATMTLQATAKEREAGIKAGLATVKAARQARLKELYEREALMYEEELNARGLSLVKPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS83</accession>
    <name>A0ABQ5RS83_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36768">
        <property type="entry name" value="ATP-DEPENDENT HELICASE/DEOXYRIBONUCLEASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36768:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE_DEOXYRIBONUCLEASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="248" mass="27789" checksum="8F966B8DD4238F37" modified="2025-10-08" version="1">MDPTRECSCSDRSSGPGYIREMSSARRVVSIILLCTSFFLHVNATTFRDGDYIHTSRRSQFHQARTNWQDLLGQHCPRFGIDRLVAVPIPKPQLSFGEKDTYKLQFSFDGDRHLTPWVPLLGQGAPTLPLVQVTLRRSGEEVLGASAEVMEAPDEYKQHHPILVEELRNVSHWPKHVLVHYRFDTRNDVDLDRGLYLLFPAALLAVLVLCLSALRGVQPKLAQFLADVTAEGTTAAAAMWKGPQGKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSM5</accession>
    <name>A0ABQ5RSM5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002424</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="5">
        <location>
            <begin position="7"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="171"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="369" mass="37482" checksum="E179BBCEF0059473" modified="2025-10-08" version="1">MDGLPRRVSCEEDVCWVCLEGSSDLSGAMEHPCACPRGVHAKCLARWQLQSAGREEERYCRFCKLELPDWRDILTPKVPAAPPVMAIVYDGKVVRLQVKPGREGQLEFQRHVRRAFNLGDDVELDCVFDCRAPGTGEKIKLRGLESYSAAMHCAAVAAGERIAKQMAGLGTGRSGLASGSSSSSSGGSGSSGSRVAAGGGPSSLSSGGGSSSSGGSGGSGGSGAAAPAASSSRHLEQQPSHSATATPRVFTPAAVPLVTAAPTCAPGTPRTARTPSSASAALAVPAIDTSPATPGTLSSPLVVESSDAAAACHVPGGVLTPRYEGGPGEFCGGDNPSPRARAHETSGAGKRRNLLLGLVRGIGAMRRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUM2</accession>
    <name>A0ABQ5RUM2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_003671</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF8">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="5">
        <location>
            <begin position="65"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="5">
        <location>
            <begin position="410"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="264"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="671" mass="74109" checksum="38C6198218AC2A0A" modified="2025-10-08" version="1">MKLDDFQRYLAELRTLPSEVPLEPELKAQRVKLDKRIREEQALAAGAVLTAVLDNGDSEASPREGDMVYIHYSIRNLEDELLYSTRSDEGGSGQAFAFLMEKGVRVPRGWELAVRDMTRGQRTLLQVKPEFGFRHPDCRMTPPVRDLPQDQPLRYDITLLHWYPAASVHVYGAGEALYKRCIREAAAWESPRPPFEVTLHLSVRCPAYDGMHLTGQCLFTTAGRQPLTLQLGRGLLPPAVEEALSYMSKGEVAAYVVPARRMLPGEGGDGTQGGASSSSDDEDDDVLGVASFSATSKSRGGSRGRGGGQESVGQPGVTTASPCLVPPPPARCTQVELEIELLSMVQVRDLTGTGEVTKKRLQEGQGEFPIDCPLHDTAVRLHFKARPLRPLQPPQGLADELPAVPVGASGAGADAMAESPIADGGEEAWTYDSRDAHGEPLSADTGCGELPEGLEMALKLMVPGEVSRVVAAPRFAYLGREDRPPGIPGDATVEFEVELLDFEREGHWQNLSFEERYVLAERLKSKGNELFRKGQHKYARARYERLLRLLESTRDFETQEEVDRIDGYKVAVLGNLALVCTQLEEYMAAVAACDKGLQYEQENAKLHFRKGKAQSLFGDYEYAAETLKLALEYDSSIEKEINAELAANTERQKAALRKQKRDMGNFFKTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVF6</accession>
    <name>A0ABQ5RVF6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">leucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_003274</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="11">
        <location>
            <begin position="73"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="11">
        <location>
            <begin position="251"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="12">
        <location>
            <begin position="855"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="175"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI61012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1149" mass="127086" checksum="CCC8A809F8BC8655" modified="2025-10-08" version="1">MRSCRGSGRILGWLNLLAEQRSLDTSTFRNHYCYKIRSGICSAHMTSTSAAAPEGAKENRARRDLLLSIQGQVQKLWEEQKIFQADAPLDGDAELHPKFFGNFPYPYMNGLLHLGHAFSLSKLEFKAAYERLSGKNVLFPQAFHCTGMPIKACADKLDRELRTYGCPPVFPQEEAANEDTDQAAATPAAPAGGEVKADPTKFSGKKSKAAAKKGTGAYQYQILRQSGIPDDQIPEFRDSGHWLNFFPPLAQRDITAMGCGVDWRRAFITTDVNPYYDSFVAWQFWTLYRAGKVIKDKRYAVYSPLDGQPCADHDRASGEGAGPQEYTLIKMEALELNGKLAELAGKGRVFLLAATLRPETMYGQTNCWVLPEGNYGAFRGLNDEIWICTQRAALNLSYQERTPVRGQPELLLELTGQDLIGTPVGSPNCPHPHVYVLPLLTILTNKGTGVVTSVPSDSPDDYTALMDLKKKPKLREKYGVKDEWVLPFEVIPIIEIPGFGITAAVKVCEDLKITSQNDTVKLAEAKQQVYLKGFTDGVMIVGPYSGRKVSEVKPVIREEMVADSRAMIYSEPERQVISRSGDECVVALTDQWYMTYGEEEWANATREALARIETYSDDTRNQFQHCLGWLQQWACSRSFGLGTRLPWDPQYLIESLSDSTIYMAYYTVAHILQKGDMYGTDHSGIAPEQLTPEVWDYIFLGKDAPKDSTISPDVLDTMRREFEYWYPFDLRVSGKDLIQNHLTFALYNHTAVWASDQSKWPRAIRCNGHLLLNSEKMSKSTGNFKTLQEAIQEFSADAMRWALADAGDGLDDANFETTTANAAILRITRELTWIEECLSPASGLREGPSDVLLADRVFSNAINAAIAATRDAYERMAFREALKAAAYDLGNARDIYRLACGPDGMHRGLVMRFIEVSTLLLLPFTPHTAEHIWRNLLKREGAAVTAGFPVGAPPDTILQRAAAYIEDLIPSLRKAIAKAEAPPKKKGPATAPPPRVVAVHVFVSERFIGWQEQVLGALAPRFNSKTRTFAEDTNAAVLEAAKQDPAVSSLGEKQLKQAVMPFAKYKMEEAAAAGPQVLDVKLPFSEVAIINDSMAYLLRSLKLDALHVHLVTNPGVSEVAAGVKADTTGAYPASPVFGFVTAAADGTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVG9</accession>
    <name>A0ABQ5RVG9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="251" mass="26460" checksum="A81F6697334AAC9E" modified="2025-10-08" version="1" fragment="single">NLANAVSALAANTFQFVGRIAANASQANRWEWLSRLTQPPNAAVAGGIDLPNQPSPIPRQGGTASPEAPTSPIYAPSPFYVHGPAVVSNHLTPGAVDIPGMAPATAATTCAAVAGTSSRPPGPPHTGHEDKDMACDDPDEETPAPPTKKPRAASVRPSITGHEDQPRQVKGAGKSKRGGGQQGRHLASKGGHVQKKGPKQNLAEYLTTFKCTRREFHFRKQHGMCLRCGSDQHRVDSCPMLGAEKNKGKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVZ8</accession>
    <name>A0ABQ5RVZ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003536</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045600013" description="Peptidase M11 gametolysin domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="3">
        <location>
            <begin position="140"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="549" mass="59522" checksum="87E2BDE6C135E777" modified="2025-10-08" version="1" fragment="single">MKTSLAPAALVSTFFAIFLFDLEACAGRQLITGSGEQIANGTLLSVTTSSLPEGRDSDEFFLRQNDGQMYRLSFCSGVVSAADLAPNVRVAVTYSSIQDGVMHSCQKPRQLDREPPAAAGAGRRYLFGDEITTPIRPTFLIYIVNMCGYGKPATVTPAMLKEVFFGGGQNPSLSEYYNTCSYGQVSLEPSRVVIIANLTIPCNGNLKGLNFTFPSGNRFDTKSCGSENPVKWQYYLDSVALKKRTAIPTNFNHKVILLPAGFTATKPGCFAGAASIGPWFPSSSNANSYGTGLIWWSTELVGDLNAMFHEVGHTLGMAHAAAVANCSDDTGQCDHTCPMGAITSRQGIRCLNAPHLWQLGWGQPFRQLTDTDLPIGTKQVLTIPPQLTTSKSFVMVDLNTMQGNMKLYVAVRINTQPYDLPYARSQNGQPFVVVHSYNGTAKVSYLRTILLADVPLGEGFIHSSSGLVINFVDWDSRKGASATFCQRAAAMEQKCGDGIDDDCDYLPDEQDPDCVGKMTFDTAPLPPHPWPPSLPPPRRPTPRSPPLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW32</accession>
    <name>A0ABQ5RW32_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein O-GlcNAc transferase</fullName>
            <ecNumber evidence="3">2.4.1.255</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_003789</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.255" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051939">
        <property type="entry name" value="Glycosyltr_41/O-GlcNAc_trsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029489">
        <property type="entry name" value="OGT/SEC/SPY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44835:SF1">
        <property type="entry name" value="PROTEIN O-GLCNAC TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44835">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13844">
        <property type="entry name" value="Glyco_transf_41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="586"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="662"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="696"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="737"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="771"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="805"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="O-GlcNAc transferase C-terminal" evidence="10">
        <location>
            <begin position="949"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="domain" description="O-GlcNAc transferase C-terminal" evidence="10">
        <location>
            <begin position="1130"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="214"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="302"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1266"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1273"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1292"/>
            <end position="1301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI61429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1436" mass="150598" checksum="04D048EC5BF6FB8B" modified="2025-10-08" version="1">MPSSMADCTCEGCEGNQRPHRQQSPSSSLPVAAGSPPRRLSSNGAAAAEALASADQMPSGNVDGYIAGRATPVEPAAKPPCLPLGNGDNNNSCNNCIEVTQFPDTIKLSNCDRSGQCRPTELCCRSVALSCGASAAAVERAALLPQSQPLCVGGSELFEQRALPLVAAIEAAATTADAASPAQAPHAGGCSAGTLAGMVVAEALQTYPANFFVSSGRADGPPPPQRPDTPSSYETHSGATAAAAGVHGHGHSHAKESPSSVSRADRRSLSYSAQQQLYLDPQSPTLLHQQQQQIRQIFRSQNQQQLVTRNSGGSGGSNISPTEASGWPSPVAAKAAGPAASHAQSWGMQAQLQQQQLQQQQQQQHQQQQQQQQAAAMMAVTGLPLLALPTPTLPYTPWGPVLPVVHVQPAAAAPLQAPTTAGMAMPPFSPLSMVAAAPSPQAPAPAGPMPPIAATNIGLPTGPGAHHGAVLQLAVNAAAAVSVAAAVPARPASAPSLSAAAPGLSAALALGSVDSETLAGQCQLAGVLRQSGKPAEALALLDAVLLRQPSNVDSLHQRAQCQQALGQVQEAISTHLRALALVPDHTASLTALGALYQSQGVLGDAVAAYRRAHELRPDDAAIREGLAVVLTDQGTKLKNAGAPLSEAVSRYQAAVALCPNYAPALYNLGVVAGELRQGDAALEYYRAAIAAEPRYAQAHCNLGVLLRERGRLAEAVAAYQAALAAAPNFTIVRNNLAIALTDLGTHVKNEGRLDEGITLYERALSYAPRHADALYNLGVAYGEKGDLQRAAFMYEMALAFNPGCAEAHNNLGVLWKEQDNIERAVECYSAALAIRPTFPQSLNNLGVVMTAQGRAADALTLLTAAVNGSPSYTEAHNNLGVLQRDVGCIPEALASYARCLELDVHCRNAGQNRLLALNYIYPGEDPLVSSAHREWGELFQTTVQPLPPLGWESRSWDPDRPLRIGYISPDLFTHSVSYFAEAPLTHHSPARGYQHYVYSCVPKPDLKTVRLRAATEAAGGVWREVARLNEAELAELVRRDEIDLLVELTGHTANNRLGVMARRPAPVQLTWIGYPNSTGLKAVDYRLTDDVCDPWDTKQTFVEELVRLPGCFLCYSPAVDAPPVAPAPALANGYITFGSFNNLAKITPQVLRLWGAVLAAVPRSRLVLKNKPFACEAARGHVLQQLAAVGVESWRVDLLPLAPGNAEHLATYALMDISLDPFPYAGTTTTTESLFMGVPCLTMAGHCHAHNVGVSLLTALGLHRPTKHQNQLPQQQAAAPASPSSAAPSATSDRHQQRAGEAEDSSMSSSPLSPGKCAVVGAVCASGGIAAAGGLISRSGWVAHSEAEYVALAVAHASDVKTLAELRYSLRERMLASPLCNGPAFLRQLEGVYRALWRRHCFTLSGDSAAADAAAEAAGLRQPLLAAPAIAMPAKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWA2</accession>
    <name>A0ABQ5RWA2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003833</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM98 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018797">
        <property type="entry name" value="FAM98"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31353">
        <property type="entry name" value="FAM98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31353:SF1">
        <property type="entry name" value="PROTEIN FAM98B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10239">
        <property type="entry name" value="DUF2465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="460" mass="47615" checksum="DFB3D893237318C3" modified="2025-10-08" version="1" fragment="single">MDREGRAADLLTRIELCGLQTGVNSFDLYSKDVREVFRKLCAALADAVQLTVTPLALCDSSQLSAAAQAVRAATASQRDAQMNRLDIALLALLKTIDATTQAKERVEVLEQLMSCLQAARLVAVSRDGGKPTFGTDRQEEPQQQETTKVSQAAATHIDGNSGGVAAAVVGKELQARTVDVSRALRLAAGALQVPLAADPGSGSRRSLPLLERLVPQVQAALDQLGPSFLAPICQRKDLTTEQLAVLADINTAFQEEYELRRAVLVERAKVTLQAFEWSGRLAERGTAEEAGGVAAAARRAMAVQPQVCLDDVWSLRPADAVALTSVPTSTPIATLDAAATVTGYSPSTDLCGAASLSGRKRAAPLAASIKSVIIGKVPDRGGRPEGRSREAYMPDFRPRSVGGGGGGGNYGSGGGGGRGGGAYRPQSALGGPSSGGGRDRGAERGGGGVRGGPQHTGSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWT8</accession>
    <name>A0ABQ5RWT8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004159</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF169">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="SBP-type" evidence="7">
        <location>
            <begin position="1017"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="378"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="494"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="690"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="947"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1086"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1264"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1360"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="972"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1086"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1097"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1363"/>
            <end position="1378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1397" mass="141610" checksum="EA9981A69E5470A1" modified="2025-10-08" version="1">MDAENVVAAAHVYRDASRGIYSGLQAFLNDPRLSDIILVRPDGLQIHVHQVILAASSRRFAVLLANGDATGKELPIQGVDSVALESVVSFFYSGECLLTPATVIPIYDVCLKLGVPGLAAGCEQYLRQNLTPQTCSTFLEASVQLLLEQTIKQCLDYAKSRFNDVIATSTFQSLSSEAVRLLLTFCRASAGEIGLARALARWAVRRPEHLAECEAMFKELNITTTVLLQLLQSPDFVAGLQAANPAAEAGTGTRRAVAGTAGAAAAGTTPVEAGASYSGPAAARVSGGVARLEAGLGNLEASAAGLPDREATQKSSRFVATDGGATQHVVNQQVPGGTRGRQLQHIIQTGLGSAVSAAGPLSPGNSSAGGKAEVLGYGGGGLGSGRDRASSGGRPQPLSFSQGERCVPGPQEVANAKRSLAAVDSAAGAAGGAGGGSRVALLQQQVFHGRLGRQRVMEEAPPAGDGGASSNAHVDFEAAYLPQPLQEMGGSMASTAKGGGGVCGTSTREEAAEPPRMRKLEAAAAVATVATIREAVRNAAIDVVVENPQSQLQPPFLGLGLDVAAALADDGDAASPRTADSGGNAGGEALFGVRLPALEQAGLTLHLLPSGAVAASGGVLGTAQLPLQVVPAGAGPHPTLLALLPRTVMVQLQQPPGRISEETCREAGTEQARQQSELSRVTALPPLVALPPLLPKQQPSMGSLLEPRGGDRGCLGPGLGPSGGARLPLLLNAVADEYRANGGGSADSVLEGELGTDILDLRVLGVAGGGSGTGHSGRLLLPSRLVPSGKEGAGAAALSTGTYASSASEGLRTTTAAAAQQQPDSDAVGGLEILSAAAAAGGALTPMAPLARGSRTGASSLHSGSGGVLAAGDVGRGLPALAPQYEPSELSGLVQQYRSSHAVTDSGGTAAAVAVAASVAAQLVAGRVAGGRPASMSLAWDEDPAVAAAGTGGGKKRRPSPAEGDLDHIEDGAGGCSGSRAGGGGGGGRGGGRHFHGGRAWHDGGQGGGGAAGNKAPLLCHVPSCNVDLSSLKEYHQRFRICDLHLKAEHVVRDGVLQRFCQQCGRFHTLSEFDGTKRSCRARLLRHNARRRKRPEHRGSGGERCGNDEPRKAQRSPSYAAAASGHTREAAAPSLLNTPTQLAAFRDVAFAAGLAAAAHGTSFLLSDPDCLGLQQWPYELAGLPRDAAAIVELGASERAPNQDRAGQPSKIFSEEVLVPGTAAAAPTKAAAASGASLMQPMLGGGMEPLQLCAAGTLDCRMKEPEMHSGQDPLRGARQSARAAEGVHPSPGDGTGRDQPMTGSALHSGAALTCPDAVPAADNAERRKNMLNGLDGASSLDEHGCPADGCQAAGRLPPAGAARSTSNGCCFNGNDSQIRTRSRAKNDDEHQRNRQAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWZ9</accession>
    <name>A0ABQ5RWZ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">YEATS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004177</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16910">
        <property type="entry name" value="YEATS_TFIID14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573:SF1">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="YEATS" evidence="5">
        <location>
            <begin position="14"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="205" mass="23926" checksum="BFDD1CEFA25E5E45" modified="2025-10-08" version="1">MDDGTFIDEHGDRRLRDTEMVLPVVVGTCAWWLGKKASDTVTHRWTVYLKAANNEDISHIVQKVTFELHHTFNNPHRVVLQPPYEVTEQGWGEFDINITLSFTPDSREKDVSILHRLKLYENESTPNTTKKPVMSEVYEELVFIEPVEAFYRRMTSTIPRPAPELSCQPYTSQYDEREELDKLKDCRTRVSAMMSDVRKEFEGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX40</accession>
    <name>A0ABQ5RX40_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004688</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058535">
        <property type="entry name" value="MafB19-deam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF179">
        <property type="entry name" value="TRNA(ADENINE(34)) DEAMINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14437">
        <property type="entry name" value="MafB19-deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="409" mass="43835" checksum="EF34FA14DCCFEC81" modified="2025-10-08" version="1" fragment="single">WRLLGATMYVTLEPCPMCAGAILQARLARVVYGARQPRLGADGSWLQLFPRTSGSSADLQPCGAFDGARKRDDCECDGLGYSSDWESDAQCERTWSSRGGGDHIDSDIGGKGCNGRLGGNVDPDQCQDGETCAASSLDRDAEGPDQKLRTCSTGIGDGRYWAVELAARNCAWDQDSHEGAVNGSGRFHQSDTGCSQAGDDNFYDSVSLSGSIDRTLHSNGRHSVSSCGNGGAWYDAPDGGDGGVADGNYCSISDWGGSGHTGNASGVGDGRVGWSKECDENRLDLECEIHGNGCGKHRMRGAIGDGRGWVNPAVLHPPWHESVVQADSTARKGRRGKDKASHGRRPRLRGGRAAEELLPLQPHPFHTNFEVQGGCLADESAELMRSFFRRRRRECRRDRSDVGLEVAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXD1</accession>
    <name>A0ABQ5RXD1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004357</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF56">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="117"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="423" mass="46784" checksum="870B0C7C8EDB4EFF" modified="2025-10-08" version="1">MEPAARHKRIWLELISQAVAIGAMLWGVKVCLHYIDPYREQREQARKRAAFLKQHLGRSLELNEFEQLLAAQVINPDHIEVEMQDVSGLESIVSDLETKLLYPLMRPELYRTTLWKQTKGVLLYGPPGTGKTMLAKALAKQSKCFFLNITVSSILSKWLGDANRLVRAVFSLAAKLEPCIIFIDEVDAMLGKRGSINEHEATLQVKTEFMQLWDGMESSRGQRVVVMGATNRPWMVDEAVLRRFTLMYEVGLPGQCQRKAILLGYLRKHNLEVPGSVSEELLFAGQAYPATTSSSSARSGQAEPDCTSGNSGPTALDRIAEATEGFSGSDLLELCSQAAQRVLAEHLQDLERGKESEAAGKQALRMRSLRLADLEDAMQHVRPSLQRADEYSRGMASAAGSGGAPVSPNMASLLALMMQHMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXR8</accession>
    <name>A0ABQ5RXR8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase beta-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="17309" checksum="FC80F38A3E9F2DB2" modified="2025-10-08" version="1" fragment="single">MLTTTATVKTTVAGGGSKRFQKFIPCAVEAHRRAEDEAGRREPGIRNPGNLELVYFLRAVKQLEENNERGRSGWAIGLHKAAQRIAQHPTRIRNPQQLKAVHGVGDFTSKLVADVLWREYPPAEPDSEEEKLEEELRQATMTAGPAGGGGGGGDGGRGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY98</accession>
    <name>A0ABQ5RY98_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="677"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1053"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="678"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1055"/>
            <end position="1071"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1243" mass="128173" checksum="0E2B2FC7465590AB" modified="2025-10-08" version="1">MAQQSQPVVPFNALKPATWGSLKLLPLALRSKLTGTKVSDDAIRVGIGKENAVGQSVILQAPPKLGDLSHLHAIGPRRSVNQRYVLQPNLSVTRPMLAAHAPGLSSLLLPGMAPIVTQHSKQQHQQQEQATARQQTPMRQRSSTPSSPSLRGRQPQANPNFAPPASNISTRLPTPVRINASPTPSPTNVAPMLPKPPSPSRSLSQVGAPLANPFACASACSSPSASNTISNTFMLPNPPSILTNIPPPRVTPVQPQQAAPRSILSPVPWASGPAQSNVTPTSVRPSPSVPERSILASVQLINQQPQRSADADASAMYTSASTAALPGHSEWLPPVAAPAPSIASPVLSAPGIQAALQQALQGAVERKPEAFRVDNFESFFDSMMHPGDTGKLDGPLPHELTDDIRYSDAWYKHCREWNMRNKPITPYSAPVSAVCQTTSSCQFASTPFVVEKLVVAQQPKQQQESDGHGLQQQQQWSVEDAPMSDACCKALDIAHHGLSKVLTPTSRSRSASDMTGVSEGGGTMHQQPFTQCTIAVAEGDCKAPSGAQKVVCTAAALTSALETLARDQNPVATTAIDGAPRGDDHVGLQRRLAPALNLVASGTAAPLSAAAVHEANNSGLDNSAPADTDAAAMITGAAERCAVQVLNTALPLVRRKFAVPVLQLSILDAPMLPRASGAALSPAPDSARPPSTAATTPGPRTPTGGAAESAATTAATTPVGRDAAGAPTTPTTATARSNAAVSPFFESTNQPVLINQEPHAAAAAVAVLAAVPLRRLSHELAAAESVADRPSQAVQPGVPLKQSSSLAAQHVAPLTQTREPATDLTGAYTGTWSPGFPPPALAPPAAVPSTSTAVITVAQQRPPSPVTLLQNAPRDMPTPNPAFFNSSTAAPGAGASSLAAAVTAVAELAQSRGPLEQSSAGPSMSAPSAPADPLKEIVVGIEKLSTLKQNILAEDKEVDVTSITQMIQLKLAVEQLLARTSSAVQDATSSQAVPSTVEATQAGPAAATLQSAAAAQAEQHGHVATPKKHSRTATFGAFLKSVFSPRSGSASKAQATAATTAPAATPAPVVPSTLGTNTSSLAAAVPASPSTTAVGIRAPRPMSASALPGNVPSPSLADKSRYAALHQRAAARLAEAAAEPAGTRPAQAAAAGAAGQGQRGGVWQRYEEKIALHRHQLDTTKAQLDEAVRKLNDMQAEFDELLLCLGMESAKNKALCDAMRAAGLDPEPILAAIEEQWMSGVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYA6</accession>
    <name>A0ABQ5RYA6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_005135</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47995">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47995:SF18">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="123"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="125"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="169"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="169"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="47"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="244"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="549"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="774"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1185"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="247"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="318"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="555"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="805"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="835"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1185"/>
            <end position="1194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1226" mass="127446" checksum="CD8DF8C9CD241CF8" modified="2025-10-08" version="1">MYKETIVMNEFNLIVSACCPTLHLCPFYNGHTLFLHFSITKRRLNKNSEPRAEMEKWRQGAEECESPSMSTTATDESSGCEDVLEHEATSSRRGPSGARATSGSHGTSGSASLGDPLARTPYRAWTKEEDELLRTLVDAHGANKWTEIAGGLPGRTGKSCRLRWVNQLRGDLLTGAFTPAEDQKIVEMQATYGNKWSAIARELPGRTDNMVKNRWNSYLKRRVEMGRAIALSLGLGLTVAPSPPVESANTDTIFQQQMLVTPSPRRRRSGDGWATATSEGRERQRRSLEQNSGAATSSIGGRSGGGGRRSNSRGRGAGSRSGTSSLSGCGQQLQQQQQPAASSAVQASSGRAPTSLLSSQVASSLSALQSAASAPGSTILSQSPPFRFAAPVLTGNPAPQPLPVWHPGIPGPHVSNHPGAVPVFRGQCPHLQSVGGPSLAGITEAVGATIPTVGSHVLPMPPAGLIPNPMLSAGPGASVSASHRSSPSVIQQAGLGAFGGLPAPGLVQAAPNAPDVGIGNGASIMSTPRVLQLAHGGCANLGTVVPHTGLLGEHPLQPQAQPTAQQQPLEGLPRQPQQQLQQPQALANAASGFTLLQGVPQQPTPTPAPFDRESDSANAQRLALLQQYFMRHLPAEQAAIAAAAALNLFQQTAAVEAQRAAVVTNQHHPQQPPAIPFAMQGSGGLPTVPEVQVAPQASQTTPWMASAPGTRQSAGASAAAFPNLPLMASACTFSLSGSAYGAHPTAATAAVPMPIPGAAAPPVFVAAAASTGLSGISQGGSPPKRRRDGRAQPAVSFADLRLTRAFSTERPQQRHLQPSGRSLAVQEHQPQRSNEPVHDASSRRCGRNLDKYPAPQSCPEPSSRMPRSDGQMARRQEVAAPRHALETAAATAARSMSATSSAPADVGNELMTDSAGLQLTELLMASPIREQQQCAAHEMFILSGSSPEGSPPKGCAGVKPTERAGTARVATASGATCLVPTQGASVTAAGGDAGNPEQQSLESHPTAIAEAMLWEAESGTCPRDNAATTAIAGGSEEGKPVASVAATFPAAHDIQCPTSFIPFEGPRRGSHSGHYHHHHHYHPHHHRAIGQSYGKYDGLTPHGSHDVQYAGQLEFLGHELCGFLADNPMGIPTPVDLSPMTGSMSDLAAAAGDDDDAHGAVDSLPTDWHLDSLLDKQHLPWSTAELATNGSSPRDQPVEGNAVGDGLCKSNGGSGAGLGQQALTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S009</accession>
    <name>A0ABQ5S009_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphofructokinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_005961</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050929">
        <property type="entry name" value="PFKA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005301">
        <property type="entry name" value="PRK06830.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45770">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="6">
        <location>
            <begin position="171"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="523" mass="56408" checksum="1BC1C801F1EC1D92" modified="2025-10-08" version="1">MRIQGPVHSNVSVIRQRIVRVPPALCRSARGRTCMQVSCVAETSEAAKQVPKPTAGLSTGAYVTNSFGAGRVSRDSVDEEDVLELKNLRNYLVPRDSPFIVDNNHGGGFVGDRDRIRLHTVEFESADSAGSFCADGMMTQGDEDSCILLPEWAIRCGPRKTIYFDPKQVSAAVVTCGGLCPGLNDVVQNIVYTLTDYGVPEDNILGIRYGLRGFYERDAKPITLTRKYVDGIHLKGGTMLGTSRGGANVKEIVRRIDLWGLNMVFVVGGNGGNAAANAISDECEAQGVVCTVVGVPKSIDNDILLIDKCFGFETAVEEAQRALLAAKVEASSARNGLGLVKLMGRQSGFIAMQASMASGVADVCLIPEIPFRIDKLINHIAAVFEKQGHCVVCIAEGAGQDILLNGAGGTDASGNPILADIGIFLRNEFKKHFKGDADIKYIDPSYMIRSVPTTSNDRIYCKVLGQGAVHGAFAGYTDITVGLVNTHYVYLPIPMIIQAPRKVNPKGRRWNRLIAAIRQPDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1Q6</accession>
    <name>A0ABQ5S1Q6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="159" mass="17925" checksum="9417128A1F0662BD" modified="2025-10-08" version="1">MMVLATNAARIHQKEEIQAARRRQAKSPQPSKARNSSRNATNSSKAAAKKVTNDGNVRASMKVRRTSTGIKRKVPVEDKSTDGDDNNDSNDHRRKKVKSASEDVRCTRARRQRGSSGSKKKQELFEEELSKDERSEDEQDDATSAREDDDNDDSEIEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S297</accession>
    <name>A0ABQ5S297_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1240"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1709"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="661"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1899" mass="198332" checksum="5E0DFFD493342528" modified="2025-10-08" version="1" fragment="single">MEIWSNCRSVIGRTIINKAPSRRITWVHNLKLRVGSIANGNGDLWPAVEYGSAKGFGSSKRGRTQRQQLEDAQPSTTVTRDRSPVARPPHQPPKLESSGRLRKNPGRGRQADRFQRTSPSSSRGTSSSSNKGTDVPDVPDGAGRMSELHAHILSTTRWRSLTDLLIRSQSSQTPSAAVTSNDGVGPSTAANDTSPGARSTLIPEPLATAPTAAAVAAEGQQLTESALLLILWRIVCTQLLTFSQRRQQSLRPGLQSLPRPLPLPLPVTSPLGEDLWQQQLWSGTSTGAADPWDLQSKSSFMALAGVGGSLSGMPRLTKRSLASPLSSETAGRPGGRVGGFRNVVGPATPPAPLVGSSVARHRCLEAAEAAARRLAPGMSRGGLVVALQLLGYWAAAMAEGADTRGLGGGEVWRLAEPFLVAAATAASTSQWVPPDLVALVAALPPLHRFCPEGVSINRGRDMGKKRTKARGQTENVHGGRSSSSSPEPLTHAVLAAALSALARRADMVVPSPSELTVCVAAAAACGYVPQPSWLALLRSELRNQLFLLTAPQGAVILDSLERLPPYQPPRCQEEGQKRAVEGSVEEAMLPESAAVELNEEQGEVAMLWAEVQGKAAALDLGSAVEALLELSQYVEVGLVLPTPAASRALLGSQHPQLAAQPPKPPKPPPPQQEQEQEQLIATVGVGVGHGVASSTACRRPSPVQQLTTVLLGQLAPQLAECSGEQLTAVLVAAARLYEYEPDSGNYAVGVTAPGQAVAGPANVSRLDSQQQQLVSSHLILEPVARSLLRQVRSSSLSLPQLGACVAALVTLLTSAPTTTSPTAAGRRQPTATPAPAPAAAAALAALLSEVPEEVLRCASTTTPGRSEVGSGRRSRAAATLGALGPDGLAWLLPLETSLVAMEEHLTPRLPLLRQVLLQKAQQQQKEHLEDVAREATETTSWGVQEWRLATGARGLAARVMSAVMLSLKQLSMAQLLELAAAAQHPQTSGLFSADAQRDVLLAILNSPLSSSTAAAAAAAAAAAAAAASASIPRRSSTSPSPSPTVAELAAVVPGIVRSLPPEALVTVLCAASRRCTACADGRDAEGQPRLRQEQAGSLLRQQKHRQRHPPPDPIAVAVLSPALAQLLDLAQPESQPGFMDVPSRPATAALSADQWCDALAAVAQAAHALVPRGPGTASDTLRGESVDMDSPNAIRPATLLQLLRALQLVWPKMRPSQAVAAYTHLADLHETVQLLYPSSAATRRSGDGGGSGGAAATAGNPPTSQADDDNSGGADSAVATATPSADDWYVVQRAVAAAAAGLTARELAAMVTAAARLATVAPLCDEWMEALTQVLLSPPPVPAPLAGGLAALDAVSALDLLRGCVELKAVAVEQDSVMLPVLPLLEHLGTALLQQPTAASAAIPAVPSEALAPSSGASTPPPPEPAVSCSAICRLLPRLSLLATDPPLAAIIGTSALPLLAYLQHQGFVGARLGDFLAALTGTSRLLLRPEPAWAAALCRATTPVLLNPATALPDVATLAHAMAQAWLLPTPEWHSTLLAATAERMARGGGGATTTRKDQALEQLLWAVGMWSQLHAAGVGPAGGAGDGKGLQVPRSWLDQVAGASVPLLAGAQPQVLASMPYVFNALGHDPGPDFIAALLQRCTASLPAFTGPQLSRLAQGLAGLGYQPEDSWVTALQLVAGSSLHDCDAHELAELLEGVLLLEERRDVASSGETEDAEKAGDGDELQGQASGRSNDLDLDTDLDSDSDLETDLDSRLDGLNPDLDWDLLLDPDLDLPSMELFLMAFWDASAALLSADAVLLSEAVARQLRRQDLQMVERLKAEFRERTTHPETGPFWRYWTEQVPFLDPSLAAPDDIVQGELAELSTPLFDGAATDAGSLQRLHRFLAWTVLRRGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2G1</accession>
    <name>A0ABQ5S2G1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006706</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04309">
        <property type="entry name" value="HAD_PSP_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.210">
        <property type="entry name" value="Phosphoserine phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="270" mass="29356" checksum="B2697BB231B7C747" modified="2025-10-08" version="1">MHAKLLARQPLQAAVAVHRCAARHARAVMTLVRSTTQPTSCSTVLKPEVSHILRTAEAICFDVDSTFCEDESIDELAAYLGVGEQVAALTAKAMGGTVEFKDALRTRLGVMQPTRAALDAFLHDHPHRVTRGIPELVALLRSRGQEVFLVSGGFRQIIHPLAEALGIPLSHVFANSILFDAEGKYAGFDESEFTCRSGGKPAAIRHIKEKYGYDSVVMVGDGATDMEARLEGAASLFVGYGGVVVRKNIAEKADWYIMDIQQLIDALQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2L7</accession>
    <name>A0ABQ5S2L7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">VTC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006791</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="VTC" evidence="6">
        <location>
            <begin position="2"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="236" mass="26619" checksum="FB6442A73A9A7F91" modified="2025-10-08" version="1" fragment="single">RNQEPHLRTEYRRTAFQLSSTNAVRISLDTQLAMTRELGAPRAPGGWCRDPSITVRHEDVVRFPYAVLEIKLQDEDSCPAWVLALRQSGLLVEAPKFSKFLHGMALLYPQRLRNTPHWFLPELPPTLPPHRNHHNHHNHRNHQQNQNQNQPDRLGITPEVTGTGVGAVHSSRTRSGSMGWIHSTLWLRGRPRSRSATGVRAVRGGWGGPMVPAMSPATLQELSYTGDKYAHVCAHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2U6</accession>
    <name>A0ABQ5S2U6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF140">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="27630" checksum="15C536AD2B08C87F" modified="2025-10-08" version="1" fragment="single">MADPELTNWCKQNGTIFNGILAAFVSEGWRGIIATKDLQPGEVVLRVPERLLLTTRSAARDPQLVAALQRHRVGQNGGDGKVLSPHQVLGCHLLLEVSRGPDSFWWPYLKQLPRSYTSLANFVDDVVGELQLQHARDVATAAIDRAREEWREVLPLLQDLGLPKRFTAFRGWLWATSTLQSRTMYLPWCPAGALTPYGDLHNYQPPPAPYTPQLRTPATSIAASAATSPCCIASVTTPPATVGIPAENESSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S342</accession>
    <name>A0ABQ5S342_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytosolic carboxypeptidase-like protein 5</fullName>
            <ecNumber evidence="17">3.4.17.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">ATP/GTP-binding protein-like 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Protein deglutamylase CCP5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">VaNZ11_006745</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] + H2O = C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:63792"/>
            <dbReference type="Rhea" id="RHEA-COMP:16435"/>
            <dbReference type="Rhea" id="RHEA-COMP:16436"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:149555"/>
            <dbReference type="ChEBI" id="CHEBI:149556"/>
            <dbReference type="EC" id="3.4.17.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:63793"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + H2O = C-terminal L-alpha-aminoacyl-[tubulin] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:63796"/>
            <dbReference type="Rhea" id="RHEA-COMP:16436"/>
            <dbReference type="Rhea" id="RHEA-COMP:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90782"/>
            <dbReference type="ChEBI" id="CHEBI:149556"/>
            <dbReference type="EC" id="3.4.17.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:63797"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>gamma-L-glutamyl-L-glutamyl-[protein] + H2O = L-glutamyl-[protein] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:60152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15517"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:143622"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:60153"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 21">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.24" evidence="17"/>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06236">
        <property type="entry name" value="M14_AGBL5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034286">
        <property type="entry name" value="M14_AGBL5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040626">
        <property type="entry name" value="Pepdidase_M14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF12">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18027">
        <property type="entry name" value="Pepdidase_M14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="23">
        <location>
            <begin position="255"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="639"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="802"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="973"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="828"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="973"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="990"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="21">
        <location>
            <position position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00026108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="GLI63696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="999" mass="106560" checksum="36FDE5E42EDD8449" modified="2025-10-08" version="1">MAAVIGPRRAQSNQLDNKTHSSDRPHPEDAAVLLPTGLPSPVDYKSDAVWRVGKYVFRANFDSANLESVEQGFAANEFLLQTRRDCGGTENEKSTRTWFYFSILGHNTGDVVILTILNLNKQSRLYSQDYRPVFWCASQPEWQPLKLRVTYRREGDDFQLRFCHRFESEEETLFAFAIPFSYRQTQEMLSHLDEQLPFQQHGVISCGTHAHEGVMESSSASLDAGAHSSASPLQPLLVTSSRVATTADRQPAGCQAQPLPQCVADACNNSGCGGGTSPCSRIYYRRQLLKRSLEGRRVELITITDCYGATGEAESPLSGVFEHDPGPPSATFREKTVFFVSSRVHPGETPATHIFNGMLAFLLRRSDPRAAALRRRFVFKLVPIVNPDGVAVGNYRTDTLGQNLNRFYTGVPDESTQPSVYAIKTLLMHYAQQGILEYYIDLHAHANKKGVFIYGNTLGDCDAHMQSLLYAKLVALNNPAFDFVGCNYTEKNMVRADKDGASKEGAGRVALYRETGLTHLYTIEANYNMARTLNVVAPASGDHGGRASPPCNKRFPPKFTVGTFHGVGRAILIAALDLEGANPWSRLPHSEHRSLDGVRSWILTVLRATPETRAAHLPAMSDVAVEKLRGMIVSGTLRVSPAFGSNGRSRPRPSALPSGESAAANTIAGGAGCSPSSFAFGRSVGANTRSRVTHTPLPARRSNASLPTIAVHGPNVATTSPISTCSISSGRGAIAGSHGRTSNDGLARSTSLPVKSTTSLVLASSPLQSVGSASGIRDAVADLGADESEDADVPITPAAAITGDAGCAITEGPSDSDGDDEHDDRTRTASPASSSSATGGPSRLRAVESPLRSPTTSFKAAAVYCGRVTEFKFPLNDRMDSSLASGVLADVPTHISAGILGHVNAAARHRPPSAGISGRLSSSGAAFTNNVPASAPVTLLAANGAVSPRHISVPQSTINGAAILTTPDQLVPASSAKNTTKSKSTLGFVRSSSANRRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3G0</accession>
    <name>A0ABQ5S3G0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein ROOT HAIR DEFECTIVE 3 homolog</fullName>
            <ecNumber evidence="8">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein SEY1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_007189</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Probable GTP-binding protein that may be involved in cell development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="733"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="738"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="762"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="11">
        <location>
            <begin position="34"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="622"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="817"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="622"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="840"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="44"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI64028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="850" mass="93824" checksum="1622444A80E4684F" modified="2025-10-08" version="1">MVMAVIVQVINGDGQFEEKDVQQFVEANDLASCKTNYQVIAIMGPQSSGKSTLLNYVFGTSFMMMDAMAGRGQTTKGIWMSKSPKVTETTVLVMDLEGSDGRERGEDDTNFERQSALFALSVADVLLVNIWCHDIGREHGSGKPLLKTIFQVNLKLFAPEPDRKRPVLLFVIRDKTKTPLPKLVEVLEADLDRMWDSIAKPQKYEGSKLKDFFEVQYAALSHFEERYEDFQADAVHLRRRFSPDNEETLIRGDEKLPGDAFGLSIRKIWDVIRAQKDLNLPAHKVMVANIRCQEILEDQLRSFTEDQAWKTLQEASSSGIVEGFGKRAHDLMQSCVLGYEAEARYFDAHVRLAKLEELQQRLLAVLQPVYHSQVTAQQAAILGAFSKELKLAAMESGGLHGSFVAAAAACRMEAMTAFDKAFSKHLRIEGTPWDVAAESMSLAEILDSRISEVRSREVLLATERAEQQLASLVSGPVIGLLDACLPGMWPRLYAVCSEAAAAADKDLSRALKGYGLSKKEEEGLSERLRHRATANMQSHVREAALTRLSRMKDRFADVFSLDDKKAPRMWGPSDNIPTIAQKARRVAASVLSQLAVIRPPGEEGNEQGDAIDAAVMQLAQGNSASTSNSNDTALLTRQSSAASSSSVTCPTLPASRASSALDVLSAATWPGADASRVLLQPHDVRTTWREFMSYSNVVVQQALSAQQANRLANNRLPPLWALGAMLVLGWNEFMAVLFNPVYLVVAALVVLFLRSLYLELDVEREMAAGPLPGVLRLSNKFVPACHSVLRKSQDAVLSMFGSASELKQSRLASYAVASDQQGQERRRRPSPSSPSVVEFTELRRESKKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3M8</accession>
    <name>A0ABQ5S3M8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="126" mass="14257" checksum="143817215E5826AF" modified="2025-10-08" version="1">MSSFAHRSLTSSNFCQQARWAGMPGPGSRTIHRNCRSYAGYKARIASLDEQETREYKIRKLAEILGTNVEQVSLLVKLRPGLLVVPNGDMSYYEQRVSMLAFKHGLSPRQVSAMILENPQLLFQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3P6</accession>
    <name>A0ABQ5S3P6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007818</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="4">
        <location>
            <begin position="123"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="353"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="377"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="399"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="429"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="982" mass="107218" checksum="A17F58CF7EC918A1" modified="2025-10-08" version="1" fragment="single">SVCFEDGQVTMRKGGEKILTGQRLGDLYALSCFSIPRSASRLRYTQPFGGVAHGKSASESMDLWHRRYGHLGLGNLERLVGEGMVSGVRFAKEQVKGAGDAGVCEPCVMSRQRRGPFPSTGHKVDQPLALIHLDVCGPMPERTPTGSLYLTVLLDDYTGFSAVAFTPTKEAVKDKVMTMVNQLETYSGFRVKEVRSDQGGEFINSPLQEFFRTKGIRHGPTVGYAPEQNGAAERLNRTLLEKMRALLAESQLPQCMWAEAVSTANYLRNISPVSGRPCTPYEAFSGEHPDVSHLRVFGCTAYVHIPKEKRNKLDPVSRKGVLVGYGDAGQYRILFSDNVVSVHSDVTFDERNVGEKAQQASRAKKSVRWRNPVDSTPDSDSDSDDDDDNDEWEPAPHTQGGGSSTGAGGSGDNSGGPTGGGDASGAGPSGSSSGAGPSVGAAGASNNFESRYPVRDRKRPSEWWRSEDPRSVAGRVHVAAATKVSEPNIYAEAVSGAQASEWKRAMDDEINSQLANGTWTLETPPPGTKLIPCRWVYKVKHNPDGSVERFKARLVAKGFVQREGVDYGELFAPTSRSSSFRVLLAVAAARKMPIHQLDVTTAFLNGELKEDLWMEQPPGYESETPGLACHLHKSIYGLKQAPRCWYDKLCEKFAEIGLFPSKSDPAMFLKVDERGVVVALVHVDDTCVASDDLELVRKVKEAIGQCFKIRDLGEARVFLGMDIRREKDGDIVLSQSRYIEQILERHNMLDAKDRATPLAMGTRAAPVTDDEGKLLEDPTPYRALVGELNYLAVSTRPDLAYALSVLSRSMAKPTRAAMSAAKGVLRYLAGTREMGLRFPGGQGLQLDGYSDSDWAGDTVTRRSTTGYVFRVNGTAISWSSQLQRTVAASSVEAEYQALSSAVREALWLSKLSDDLKIGTSPSIIKVDSQGAMNISNNPMTSQRSKHIDVMHHLVRERVASGEVELRYCPTEKMVADVMTKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S474</accession>
    <name>A0ABQ5S474_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="103" mass="11458" checksum="DE166EF5516C5EB4" modified="2025-10-08" version="1">MDMDVSLNFEAIKDAPVEWTLQHERSLRLRRQAVPAVATQTPSRRTRNGQPILPIAEQSSSTTHSAAELPRDVDGDDAMEASDGEPPAKRVRVLTHRRLPLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4A3</accession>
    <name>A0ABQ5S4A3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="23248" checksum="C2C89C62B28E1D15" modified="2025-10-08" version="1" fragment="single">MVAKCRAVRALLPSVLNQVEQVIGRRFSFDARTVRAGFDGFCNLFEPQGRPLSSANLGGHTILFTPSSLCELQKQLALFRACKRQYPDTSACILLPEYVSRRNPSFLSGMQRVLRFNPGTALFHSLDMAGEVVQLPGSPYSVDVWFAAPKSVQEGLPETEPCISVLCLPKAAALIGAGVPLITVSTAGRTFTALADSGASDDFISASVVTQLGLKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S572</accession>
    <name>A0ABQ5S572_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="160" mass="16159" checksum="0CC34E5425C1DBF0" modified="2025-10-08" version="1">MLSTVILRIPAARQFSGQGPSRCCAAVVAPRPHRIAARYTSMQGRLGEEGEEETSLRASINKLTSEKQYAGTFRVGGSGSGASESSSANAPVNEEKEPGHHPSPTSAPESGTEGRTPGSGAGTESARTAGGGSSRYNPATPGGGDSEDVSSAPESGTVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5L3</accession>
    <name>A0ABQ5S5L3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-mannosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008654</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050887">
        <property type="entry name" value="Beta-mannosidase_GH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43730">
        <property type="entry name" value="BETA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43730:SF1">
        <property type="entry name" value="BETA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="775"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="873"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="783"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="804"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="819"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="966" mass="102555" checksum="EE6C7B5D1AD418D0" modified="2025-10-08" version="1">MTGAVRQLPLRSPQRPVPGHPTSRREFMFACNPYPNTLEFGEEVRQEAAEQVRRLAAHPSVAIWGGNNEIEASFDWFTETRANRQLYATDFVAQFVDLIRGVLQQLDPGRPYVDSSPSSGLYSRTPYIKRWGDPQDQAAGDVHYYNYEADCLDPGIYPSARFVSEYGYQGFASYDVIRRYGMASDLTPLSEFMRHRQRHPKGESELIASLGSHFRQPTGGWGSSPGPGGRGDGGSGSNGDGGCTSFPCPTSTSAAASGKQQLEQPALNDLHLYQSWIYQLQTQQALCYSTALGRWRSLRSDPYVMTMGVLYWQLNDIWPGYSWSSLDYGGGWKPLHSFVRRLFAPVTVVASYYKKRSNNVTVYVVNDGLQEASVQLTATVVSLAPKAAAPTPRPSANISSNASVDVSANAGSETKYGAAPRGGGDGRASTGDVVRAVQYAVLNLTAPPQRSISVLEAPAAKILAALPGCSETTCVLLVRSRILAIGGGGNDASASSTRNTVLWPWGRGDFERDAATLVQMAHWKERNVAAAAAAAADVGTAEKNSVDSDGTATTVAASGFNSSDNTAAAAAASPNDGWDWSSPETLVMFVPPKDLRLSPAEIEVYDIQMRTLPPYRYRDRSAGGDDAITTNGSVVNQSGNRTADTAISDFANGTAALPGAAAATAAGHSGGIGGGRVRYEWCFRLRALRAPALLVALESSLKGHFNENFVLLVLDLVDVERDAVSAPPLVPPTLQTTLQPLPQSLPQSVSQSASPLAQVLSNTTDQIQIQIQQNTSQEHFRVSQRQNTASQPLRNASRLPLPGGNTSQQSQRRATIATGGSGSGSGSGSGSGSGSIGSRAGNRVNIATVDLQRLAAAANKAMEAATTAGLAVTISPSTDSGDGGAGGSDSNSDCLEKGGKTAAAVSAPSSQLVYDNEVARTEVHPRVCFYTDEEVEGGGEEGFRATLSIWDLYSMQAFSYDNATQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6N9</accession>
    <name>A0ABQ5S6N9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024095">
        <property type="entry name" value="Vesicle_P115"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013:SF0">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="431"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="601"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="782"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1308"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1429"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1553"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1769"/>
            <end position="1798"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1401"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1518"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1629"/>
            <end position="1663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="190"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="403"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="608"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="660"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1324"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1350"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1442"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1452"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1471"/>
            <end position="1482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1798" mass="187450" checksum="C85120B5FCCAA5D0" modified="2025-10-08" version="1">MSGDGNLPRVSENGPATQKMAPQDAESSSIGGQQDPSADSLSVHPQQQQEQQQLPGEQQQPAEDRKVESSEDPKQHQQPTEQQLLSKVPEEVGTPRPTGGAEILQASLSRSSAKRKVPGQLTESSRRMKVPAAAVSSEPQQLQTQTSSPPTTVQQPLQQLSATPPLQLEQPFIKQEGPSSPSFQQRYDISSSSPPKSPSSTRRATPLPTSSSPGPSPPRLSSTAPLTRSSPPPPAGSPSFSSPRPKSAQQPAEAFTQSPSPRSSSPLPSSPQINKTPPPHRPSLETTRSPAPSVRQTEPQPVDSQPQAMLPAAPSSGSRAPGSRELEPHLRQHLEQHQHQHQQIEVDISETDDLLDPDEPESDWGPMEKAADDKEDDGYVSTTGVALERPAGPSPHQLGAGGSSSSSKITAVQSEAPAVVALSSLALRHRQIPRPPPSSYPPPPQHQRRVQPQPQPQPQQPQQHAAREAPSRLPEVQQQLAIGAGPGPVLAVDKAELMRQLYARYATAPGQPRIVTVSDFAQPRMSGSQLQRLVTALGLMQPSGPLTQETVGSIYARCKPPGTRKLNFLNFLRVLSKLHQESGGADVWGAASQLAVRINRKHQKNNKQQAHQPQQLQQQQQPQQPQQPHQLQQRRVLNPPSLSSQLEPPAGPSRAANIAFGGGSGGGSGIAGGPQNAFGEDASHLSDSEVASAPLLLPRLPAVAPNHHSNAGGGSGGGAPITAPQVIRKYSEPMLAPVAGGRGIHAVQMTSFFPTTVGSRMRLPNEPGVGAVAVPLTREAFAASPTGRPSPQPPPLRGIDDHVTSEDLPVAGRHPGALQRSLTGIAALPQGYALLATAPPLAAPPAALSAPTASASVGSVPTTSAAMVAAAETVQTLLQRVICLEEQSQEHKTRHAELRLEMLQKLAEVAATSTLAAAAAPPQLPQSAPAAQQTPDAVDAAHVAVTAAGTAHRAEVGAAAAARVANSAAATAAAAGREVEHMEDRISACEAMIRQQLEGMREQRDAVESFRDQMTGMEASITSIVMAAVSAHMEQKLQQQLEKELEKRLQERLKEVLKVTAAHEARLQQLQRAQDQAAAATSPSAAVAAVTAAATTAAVAATEQRLAGLESTAAASAVTAATAATVQRLGEIEATVTTAATAAATAAMEQRLAGIEATVTTAAAAAAVAAMEQRMTVAGTTVADAQQAANTAAEATTAAASRIDTAVAELTARLNATESQILATLRHRILSLPGAAATGRISPDVTAMAAATSAANGVLYEDREKDLTQRPSEGVITVADANTTGCEPASRKLADHLGIRSLKHIVIPAPPPPVGSSAVTDGVGNNGGDEGGGDGGSAGDGEGDAAAADQSNTSNNSSVSLREAERHALKIRVAACERMAAADALTRSMHHTVLDNHYHLLDEHTSELRRLDLELAQLQEQVHELLLQQKQRQPVTAPPAPEDGTAAAAASGDGGGGGDSGGSQPPPQPPSSSEWSRWRSQSHMQSELQWQAQLMSQLHTLQSQVDSLHGLHQSSQSLGELSKHLAALSEMYRVLEEQLEDLTSKVGWMQAKALSSTSPPRPRPASPTIESQPPIAATTAAGAPATATAVAAEGTAVGENIAGTVATAAAVAAAAELTAASELRLRKDLDSVREGLTAHTQNLEQLRSRLDAMERTHEAMATAAASGVQSLVSEQSYVTKAELEDLQRFTQSALEELSSSLRRLDGNSSDLTVDMQHAAKDVGRLLVQVRQLQQHYEDLAESIGMARYSGAGGSRAVTRTNTALVGKAATAAPAATTPPNGDETAAAPAAAAQQPGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6P9</accession>
    <name>A0ABQ5S6P9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyltransferase 61 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049625">
        <property type="entry name" value="Glyco_transf_61_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04577">
        <property type="entry name" value="Glyco_transf_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 61 catalytic" evidence="2">
        <location>
            <begin position="302"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="605" mass="68761" checksum="4AF2B1DE531A80DC" modified="2025-10-08" version="1">MSSRISRIRIEFVWIFVLLLCAVLLGFASVRRYRHAAILMLHLDQVDQKTSNLMFNTDHCLHSTDHSAHSPNSQMEDLASRSRTQFAAFLGLGNSSGPIIEMESEKWKPLILRHCSEDLDMPPYYAPCLTKVIPNLEYGEELVYPDFEAALPGFFNLKDAQIWLRTNYGPDTQGQTSSNKRVIVDERQGRLVFTGVRGQNLAFRNVRYSDSSLSHSWVPDSSHCLGHMTSTVMFHLHQQHLIGADRASRKQPTRLGSEEEEAQEVKEGEKEQWAGEGVLAAGDRLEVVEEAAVYLTPDNERYQHWLDHTSKPLMQSAHLISGRTRILPGSILESKQPIVRTMWRWLPHINQSQLSGANNDLHVRRLFMSCRVPYVHPYLFFRLQEMVLGAEAPRVPLSQRKVVIWYSRSGQDTSRQNHGRAILNEAEVLAAVRQLLQDRGLGERLEIHPPAQAVTDQLEYVKYLNRHVAALMGPHGGGLCNFKWLALHTLVLELMPRSWISTPLYEDAIGHGLNYWVDLLESQNSRHDMVANVSNIVAMLKAELGREPVRGPIIRYRYDWPAALDDSLSRDKSRSTYFVSMKGKCCNNAPGDRGAEGLHVLQENP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S759</accession>
    <name>A0ABQ5S759_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="122" mass="13819" checksum="422C61742EEA76D9" modified="2025-10-08" version="1">MSLEKPDNVVKEIENYLAQQRIDKVVKEMVVSVLHSKPESVEQHMLKHLLTKTKEAEGEVAASSTDDGVHVAFTVKEDARQQYLVSKGVHVLFAELLQRVRSSPRMWQPLSPPMCHSSKRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S802</accession>
    <name>A0ABQ5S802_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009230</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.30">
        <property type="entry name" value="Exocyst complex subunit Sec15 C-terminal domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007225">
        <property type="entry name" value="EXOC6/Sec15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046361">
        <property type="entry name" value="EXOC6/Sec15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042045">
        <property type="entry name" value="EXOC6/Sec15_C_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048359">
        <property type="entry name" value="EXOC6_Sec15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702">
        <property type="entry name" value="SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20651">
        <property type="entry name" value="EXOC6_Sec15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04091">
        <property type="entry name" value="Sec15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC6/Sec15 N-terminal" evidence="7">
        <location>
            <begin position="129"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex subunit EXOC6/Sec15 C-terminal" evidence="6">
        <location>
            <begin position="892"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="402"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1106" mass="115645" checksum="8D9C9FA95447A910" modified="2025-10-08" version="1">MKQSGGGGAITGKPQQQQQLQQQHQTQQTLSQPARSEEASIRELVESCLVNEDFTPVLRLVFSSSSAATFPNSPSRSTPSESAGTSTATKTPPPPPSPNKSSQSSRSPPRVPGATLDGRPALEVLQSVLRSIANEQDAIVHDVCSSHSMEIATCVAELDRMVASAEELRKAVSAGGEELQRTGLALASTARELHGLLSAQRHLAAAVGGLRVLRRLLSRCGQVGALVAQRRLFQALQLMEQVRTQDLAQALEELSNTPQAAAFMSLGPDDTAAAATGAGGSSSETTIGGINATTGKGGAGSAAARHLSVASRLRNFLEALVSDLTAAIEYYALAEFNNWLVGVRAESRQVGLRAIRKAAIERQMDEDMASERRTVAAALISLRDPAAVAAKEVTGALFRRELSKEGAPPPPPAPAPQSAAAAAAGLGGGGGGNSTGTRPGKSGASRSGQVQASSPELPASVPGLDLTTLCPGQPVQAIQTLLSRHAAAASVPLAPTCSLTDYTTALLQGVNMQGLYRCVHIHRCTGRMAVLRRYYLDQRRLQITTDLAPPGKFLESYQSYLAQVTGFFVVEDAVRTAAPDLLSSDSVALLWDAAAGSLRGVLGRALEEALGGYAPASIMLLLKDFMLLVCSALGARGFVTAPITELLTGSRLRYHELLTASVALRVGRALAADPLAEGLQVVSEREEAELSGRLGLPAALESDGTTPALPFRVPYTASLPKILQAVRGFVTDSLAYLQGLLSPWELLPAVLQARDRLLAKTVVDALADHTARLLAADAAAVASIAATSLTHGSVGDPLAPRRGPSLHIKEVMRMITNAWSLASAMQALDEWTLLQVRPLGAMAAAAAAAAAGGGSSPSPTGTALPAGGGGGGGGGGFIGLGSDGSTGAVLAALRSVQDGGERGLMRLLASRVEVLVAGARGLNWLPQTPLPPTPSQYSEELVALLKDTFDAAASILPRSSFLFLSRAVVRAIGNAFMALLKEGVKAYNMFAVERLAADLVPLDRFITGCPVPHLTQEFAEPRQLCALLLSPRVEEVLIPEVRRQRYPSLDLGSLVVILERYREQARVGKEKTGHVSKRTLEAVCKQLRGQMVAGTAVSGLRVLEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8G2</accession>
    <name>A0ABQ5S8G2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_009574</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC3/CDC27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="628"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="696"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="730"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="764"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="158"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="335"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="459"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="359"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI65921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="924" mass="98495" checksum="321DC31F0F6BC4AA" modified="2025-10-08" version="1">MAAEQALTASVQESLALFQYENAKFLGERLVALHNSEQNQQLLATCYMHCNQSYRAYHLLKGHNSQHSRYLLAICCLTMSRYPEAKDALLKMGEAEIPYGYAGLYLLGKVCRLTNCMSEAKDYYFRALRLNPLLWSAFEELCAMGGDEEAAEICSYAQQPTPAAADEPSPSTSSQISSALAQHTPQQNYHNHGRHGHGSASGAGKDHGRSSGQLHQPSGPAGPSVGHFRLGGHAGGAATVATGGIGMGCFGGAPYSGAHGLATFGIGVGLGGGGLFHTPLPGSGQGGLFGSPLFGSSGSMFATDLGQIGMVQHQGDVDGIAGPHTGTHMFRFPGMAGDMYSTPSPMGMGPQPPAPPQKQPQQSQGHWEASGAGPSGAGGYHGGSRVGASGGSYQRMGRMMDEGKIRKVPNNKLLSSDPRAVRRSQRLAAQNNMTAGAQVPASHAGGALGGGAAAVALQHQQHQHAYPSSYGGVNATTSAQGPAKSGQASYIEGDSQNMPDPRDPPPLPVQQQLHPDAKLGGPGAAVVAATPQQLLAAFNGQQGTRQGPGGADSLIPSHHYPEQQQQLLFQSRAAAAAAMAAAAMVESRTALLQLLAPLMESVRHLAAYRCPEALDALQRLPKAQARTAWVSCAMGRAYFESMNYSKAAQHFETARQLDRTRVEGMEIYSTVLWHTKREYELSHLAQECVATDRLAPQTWCVLGNLFSSQKEHEAAIEFFLRAVQVDPTFTYAYTLAGHEYFANEDYDKAAACYRSALKLDPRHYKAMYGLGQIAYRQEKYAEALQNFRLAASINKRSSVLQCYVGMSAAKLGQSALALEKLKEAIDLDDANPLARFERASVLASLERVQEALDELAALQQMAPGEASVAFQMGKLYKRLNNRTAAQQSFELALSYSGCSSSDAATIKAAIERLALSEDEDEEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8J7</accession>
    <name>A0ABQ5S8J7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="985"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="985"/>
            <end position="1004"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1462" mass="154563" checksum="1F58E7B9EE96A1AE" modified="2025-10-08" version="1">MVVVCEICGFDGASEWFLQCKNHERCHQGRHLYCIDGLRVRRRPASLGAFGVLECSWCLQTAESTSQSAKRNAALALDKSAVTRTEGQRKLKEGNAATAREAKPHADRPAANKVSIAEGAGPDKVPAGKVPFVGKPTAGKAASTDRCGKALDGGAAGGKEEVHGDKKRSAAGISSQRAVAGGSGVPSATATAATGTASLPESMAGAQCGRYTSKAPGAIPGRASGMQHQSRVKPATVVAGGAATMLVADELDGFYDRTLLVKQNKVAGPSARSQLAGAGQQAVIAGDSGTSKPGSVLPHGRPISTKQNVGGGLPVGAPPAGSAGAKTGGAGRTGQTGGGGAEARGASLAGAGTVFGSHAYGVSSLAARNRPTVVAAPTGRVSFHYQLALPTVSSVQSSSLSQTKLDNRDSLARGSRLGSLPLHNSSTQMVVTSRDRRVRLWTFDMVLEGCIPRTEMDPKRLRMSIESISPHESMVRLSKLLQAKTPPEGLRCSVMRIEEVPRAISEAHSFYLLYNPCETCVVMYPTRNIGNTSKFFKESLITMLLPPEVGNEQGAGERAQLEEPGKILAVDLSFLTADDLAKDPVLERLLHSELDTKGDSKTKYQLYFMPETVPVDGVTPENGVMPCLLSLTGSRKHVHLMVPPYNRRIPTEEESILINPTRVRRLKKVTFELRDNHKHETGRLLKETIQEGKALKKVLLEQGESTAADPMECDIDGSAYASEPGSMGDGPVALDPNVETLHNAVLMASSSQPAGSPVVVMHSAEKKGLLQDTSGVSDPGTQQALGIDVVLTTPSQPEQLVGDAEVTIGVTDGAHSLIEGHATVDSGIEVLCQDAKLMAPLQPAGSLSMCWSASFEGSHSAKDGSLASDKNNQQELVRSATRVAAARAVVETGMKNNYGEAEMSSEMAANNKTTVQFVCSIDAIGRVAEQQACTSALGDEALDMLAQYQQALATHAPLSDDDTSNSGGGAASVADVLFISGSNVTSDAKNNPSGSRAPATQTSGPLPMAASICTVEGTWEPLNGMASNGEAVDAARSTGIATDCQLSDWRGLANCRNPPPGLHCQMVLPIPNMPVSVEVPSQEHQWQVGKKRPRSPLHSVARQAGLNLSSCKEAAQWSWGRVSAPVLQAFLMESGCMDLACREQLLPDLIPQLQALLSKQLHGCQIRLVALAGEASMLENGLDRAAKVLGFQWSSWCKAVDCEKLSGIQVVLLSEAAVSNVPNLTGLVLKSIAVHPIALYRAEPFVKGLMLLAEETKYIHKDGRLAMALTLEDVLSRASLQGAAMVDACRPLYPTRVVVVMDAEGFMRSPVKQLVRLAGCLLAGSRTWGMPWQLQMRASHVSKIMADGSPEHWAITTWALRPDALTGGELKQQQPMLQELDLPEESATVTHEIVLMRDAAHLMYTRRPGRLVVAAYAAEDLAVKLHSGKGRRCKDILAGTVSEVVSFLDLLLSGAKITGQMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8W8</accession>
    <name>A0ABQ5S8W8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_010169</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="24314" checksum="92DD12E9985F9793" modified="2025-10-08" version="1">MADWGMRPQDTAYGQSAGVEWFSSGGAGSYGSYGAYSVPVSGYGSTSGNSYEDEPPLLEELGIDIGAIFLKTRAVLFWRFNSRYLDDLDMGGSLIFVFVLAGLQLLMGKLHFGVTLGWSVVFSILLWFMLNQLVGSAGAEAKNLDPYSCCCVVGYSMLPLVVYSTMAILIPRGPLSLAAAALCTLWAALTASRIFARRCPALGDLKGIIMYPCVLAYASFALLIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S927</accession>
    <name>A0ABQ5S927_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="330" mass="34305" checksum="5E43A7C47D12D3F2" modified="2025-10-08" version="1">MDAGTPPLPVTPAYFASCCISGGAIAPAPIANADLAQAVETDASISDASGCFPSSSFITSFVGNTPFPTVAPKSGQPVGGHVKQKYRLVSPLFWLIPPVAAFSSEGGLLCTDSVHALALARAAAGRRRMMAALEEARRRFSHLSRSKLPNSWPSTAPSISTAAQELQFQREGSSREGATGSRCLDEGSEMQRPMVAPPARLLPNLPLAPRDVGILSVGYAASIAVVPCEASKSVSKVPETQQGRAGEYGRGNQEGQYEMGGRDGGDSDGRQCANAATVGSAHMVESLVGGGVGDVPLSVWAVWMGWSLCEVVASSPSASSQLQTGCKWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S934</accession>
    <name>A0ABQ5S934_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009846</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="331" mass="35677" checksum="88490383C929B4D5" modified="2025-10-08" version="1">MSRLNERPWKVAFVTGCDSAEGIGQAVCRELVTRGVLVYATGLSLEAMALLRDSAPKQIRTMVMDVTRENQVQEVIDKVVAEAGGIDILINNAGIGALAPTVEADLSIARRVHEVNFWGTWAVCKAAGRHMAARRRGVIANIGSMASRGHTPFTTAYNTSKAAVESLTHSLRLELMPYGIAVIYVAPTWCRTAIAANSTMPSDAEWRAGLYGAIQEHVLRETKAINAPDSWPPSRIAARIVDAVFHNRPPVEVYGGGQYLALGFLAAWAPRWLRDWVIWHKSGLGHLARLVSTARNVRATAMALLPPDDDTCTIAAPADAAGTAAAATENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S950</accession>
    <name>A0ABQ5S950_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_010255</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0229">DNA integration</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI66447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="184" mass="20686" checksum="DA7FCB34E92C851C" modified="2025-10-08" version="1" fragment="single">YMTVLMDDCTTFSAVAFTNTKDAVKDKLVTMINQLENMCGHKTWEMRSDRGGEFVNSQLQAFFASRGIRHGPTVGYTPEQNGAAERLNRTLLEKMRALLLESKLPQKMWAEAAATANYLRNISPADGISRTPYELFTGKVPEVDHLRVFGCTAYVHVPKAKRNKLDPVSRRGHARTIQTLGRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA03</accession>
    <name>A0ABQ5SA03_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutaminyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_009725</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00807">
        <property type="entry name" value="GlnRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1290">
        <property type="entry name" value="Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007638">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007639">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042558">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042559">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF4">
        <property type="entry name" value="GLUTAMINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04558">
        <property type="entry name" value="tRNA_synt_1c_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04557">
        <property type="entry name" value="tRNA_synt_1c_R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="15">
        <location>
            <begin position="11"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="14">
        <location>
            <begin position="171"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="12">
        <location>
            <begin position="277"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="13">
        <location>
            <begin position="586"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="16">
        <location>
            <begin position="699"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="185"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI66021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="802" mass="89984" checksum="45E13480A9A23976" modified="2025-10-08" version="1">MATKEEIEAACISLFMGIGLEETVAKNATRNAKFSKTLEEVIHEAGAASGCPKAKGNLLYTVASKFPANALCHRPMLLGYIMCEHMKSNAQLDGAFEYLRKVGTTEVDKKSLEEASGVGVVVTPEQIKTAVAEEVEANKDKLLAERYRVNLNILLGGVTRKLKWADGAAVRSALEAAVEALLGPKTEADLAPPEPKKKSKAAAPPAAVKEEGAAESAQAAAEGPAAPAQPDDPYSFLPKPDVNNGVHTTVNFSDGRIMRIANTPEALAAHLARMGGKVVTRFPPEPNGYLHIGHAKAMFVDFGMARQYDGVCYLRYDDTNPEAEKLEYIQHIEEIVSWMGWEPWKITYSSDYFQQLYELAVQLIKSGHAYVCHQTKEEIEASREKREPSPWRDRPIEENLRLFEDMRRGLVDEGKATLRMKMDHKNENFNMFDLIAYRIKFVPHPHAGDKWCIYPSYDYTHCLVDSLEDITHSLCTLEFETRRASYYWLLEVLDLYKPVVWEYSRLNITNNVMSKRKLNKLVIGGYVNGWDDPRLLTLAGLRRRGVPSTAINAFCRDIGITRNDNIIHMHRLEHFIRAELDATSLRALAVLRPLKVVITNMPEDHFEEVEAKTFPGRDNSESYKVPLTRVVYIETTDFRLKDEKDYYGLAPGKSAMLRYAYPITCTSYTQDPATGEVTELAVTYDAEHYTAGRKPPKGVLNWVSQPAPGQEPPRFEARLYEALFKSEDPGSMENWLEDLNPDSLVKLSGCVMTPQLANAKAGDRFQFERLGYFAVDPESTPGALVFNRTVTLKESKPVSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA72</accession>
    <name>A0ABQ5SA72_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleoprotein TPR/MLP1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_010114</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057974">
        <property type="entry name" value="NUA/TPR/MLP1-2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012929">
        <property type="entry name" value="Nucleoprot-TPR/MLP1-2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898:SF2">
        <property type="entry name" value="NUCLEOPROTEIN TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898">
        <property type="entry name" value="NUCLEOPROTEIN TPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25785">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07926">
        <property type="entry name" value="TPR_MLP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUA/TPR/MLP1-2-like" evidence="7">
        <location>
            <begin position="480"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoprotein TPR/MLP1-2" evidence="6">
        <location>
            <begin position="1071"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="467"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="655"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1582"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1845"/>
            <end position="1868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1901"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1935"/>
            <end position="2110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2124"/>
            <end position="2345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2368"/>
            <end position="2426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="214"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="697"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="778"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1128"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1225"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="486"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="680"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1586"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1610"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1636"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1950"/>
            <end position="1969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1988"/>
            <end position="1999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="2002"/>
            <end position="2014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2043"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="2077"/>
            <end position="2086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2124"/>
            <end position="2141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="2193"/>
            <end position="2225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="2233"/>
            <end position="2244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="2270"/>
            <end position="2280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2401"/>
            <end position="2417"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="2426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI66334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2426" mass="255592" checksum="5490B7B081EBF078" modified="2025-10-08" version="1" fragment="single">MVDSERRVADLEQALQAANAQMEANDMSYSLALKHYQQNEEHWSIEKRQLQEKIDKLGVDLEQRSQVQEELARVAHELRGKQRALDELQGALSVVTEQRSNTTKTLDSLRTVLVAKDEEVAVLTKQKADLIDLVHQLQTSLNSNDNTRQLALLQTQYDQDRRFWESKLKLLEDDLNAKNNLLLDDRRRASGEQLALRQKVAELEYSNQVSDNARKQLEAQLRELDGFLRDARKQAHDLEQEKIDVEEHWRRQATEYQHLTMQHQAARMAAEEEASALRAQLGDLQGQLQVLQAISDEAANKVDALQSAYGSASQRHKAQIQHLNAIIAAAMSSGDVTVLTNALPPGSPEAAAAARGAVTAGRSGAAIDPVTPGVQLVANTAGTTPATTLVAAGETVLQGKTVMEVWAMYQEMQDAWHQERAEARKMRSFMETIAADLEAKMAFSARQQEEADKVREALDQSMATVGTLEESNRRLQDSLSTAQGSLERKESEKRSQDQTIRDLSQQVKVLMEEVQRLSGKPIARSSSGSMGSAGGLTVGNRVTADQLISATDVINHRLVTFRTLDELVEQNRRLLTMVRKLGAENERSREEVARDVAHQFAAREQEHKSTIAELQQLTAELTGATSVSTKRISELTARTVELEAALVAAKAAARGASTIGDGGGGTAPVSPGARSPPPGDAAGPADDGAAAPDTADLARANEDLSQMRQEMRSLVDTMQTQVNELREKLAAAASESARYAGRAEDWRQRCTTLESLRESELAMRNALAAEKAQLSALLATAQHKADMAQQSVEELQAQIRTRTEHQVVLEARMSAQTTALEDARSALTMAETERNRALADAKVAEQLRANSEATARMQKDDLQRQVASLQSDMARLKEDHERQQTIFHHSVEAATAQLREVRDEAGSMRRARDDAERSLAEARDQVVDLRGRIAELQAQVLDLTSQLAAARGGAAASADLVEGQKLRDVEAQLREAQADLASAREQLEARNSALETLRGAMAALQGTYDALRESHEAAQTAAKQQAEEAEAARVQAQAALAAAEAAIAESRAALAALQAQHEALQASSAAEAARLSSEVERLGRELADRVSQRTRDDEDIARLQAAVREYRSRYEEAVGSHARDVSALQTAETRLEEAQASVNRLQNELTAALSGVDERLATADREKEALKAELVAAEARLADRITANEQLLTSVRGLLAGTHHELVGMSAEQYKSLMDYLGRDKALAAAQLELLASERNKLREERDNSVRQAAVLRAQMASLQDSLTTSRADLQARINLLEAEDHTRRLAQGQLRGERDTARAESERLRTLLAGAEQRAGAAEASLRESAANVAGLQAQLEAHKRGEALWREKYDALMTRYGKVTLEEHNTVKAALATVRAELASASEQVKGLQTSGATLQQQLDELRTSKDATVADLEKSRKEFADLRVRHNMLVRKHRELTAANEAMGKELAAAREQLAAANDKTAALEAQVAKAKEDTAASVAEATGKIKASNKQAIERIRTLTAEKAAQEGELQTLRTSLESLNKTLEEERIRRASDGRAAAMRVLDLERQLKTAREELASSQGKVRELTERIERARARRVGAPTGAAGPGPSSVPTIVAPGLSVGATQPTVAAPAGPPPSAQLPAQLPAQSPPTVPPSEPFASAASLAAAAAAARGARRTPIQFPTPATVQQSTVQRLITVSDQVGSGAAAVPVAATGSVAAAQQSPPQLSTVVSTTGAAAQSIDAAPVTTVEQQFGLSVNDASGPRATGASTLAGPDTLAPAAGGGGVGGNDVCMAEASQHPDVVLEPVQPLAMRPVPLPSPQQGVLVGTVTAASLEDTIATGSQTADLGEVPADMAVNPHMDTQPPTHAGATGTDAEMGDAGAEPLEVGVVATNPASSADPVTHAAAAPTPILEEPVSQPATAAETSAAAEERIPDGANVVAVQEQQDGGETAGDGQGNTEEGTEEGSEEGEDAGAEEIVVEGEGPADGAMEQGEIGDEAGAAACGSTGAGQDAPEEGELHEEQDLGAEGVAGDDREEQVATNTATVEVGVDTLPAEAQNAEEAPPGTGQHEQEDDGEGGGDVISGDGAEVEAEEEDGGGTPVPPSEPVGGEGGTPGPSPVALAAVDAAMAGINAAAATRDAAAAAQRTATLAPEDVAAAEGSAAPMEEQAGLAAAGRGEEDGGMADQPTALPVGGDIDAHQGGEDGDGDSEDDGDGEGEGEGEGEEGDADAEDYEDGSSGSSGMDDDDDDDDEDPDLKALRLKAIAAATTNTRKRQMTPMLEDHCDGEEAVRMNQAAAPGDGGGVGDGPPAPKRQRSASLTSLAVLSKVPASETEVDGQPEGKADGGAEAEESDLELDDLVPGVVADLDSRDAAAAVNATTAAEGGTPSQGACAPATSPGPPNEAAEVTDAQRSLPRSPSPPQQGSKRLQLEEEGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAE7</accession>
    <name>A0ABQ5SAE7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF4476 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_010475</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000037">
        <property type="protein sequence ID" value="GLI66639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028011">
        <property type="entry name" value="DUF4476"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14771">
        <property type="entry name" value="DUF4476"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DUF4476" evidence="6">
        <location>
            <begin position="1497"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="26"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="181"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="235"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="503"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="594"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="907"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1419"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1765"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="524"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="604"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="661"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="923"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1423"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1765"/>
            <end position="1775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1799"/>
            <end position="1816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1855"/>
            <end position="1865"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2102" mass="216638" checksum="9A6E86E16CE4266B" modified="2025-10-08" version="1">MQLHTTTGVTAAGFLAPGISATAVSGHADSTGQLPPMGTRRNSLGALGAVPTTNGGGSGSGAPVVAATASTGTAQPNSSLGRMIGPVAMTGPATPRIQTGAVGPGPRSPAISSPGGGAGAAARGTNVSILSAGVGGGGFIAGGGTGGRPSSRTPAKDAAAVPRKVAAVDNIAEALGRFSQESMELAPKPPPRPPSSTSSDDGDLGPISSDDEATQEELDVLEKLYLVMPNLESPGKGAGGRTASPGHGILSQPPSRVSGSFTNTVGGSGSRPASPLQHITASFGAGGAPPGHVPPLAPAAPGTATAAAAALPAAGPAPGRSISPSPMPSSSVTAVAANAGSPTPLDAAVVAAKRKALARKGAAAPQQPTFLPSKAVLLFHQRALGITVPLANSTNGPGAAAGSGGGAVIGNGIVSGIGGGIGGYNGPAVAVATTGVSGPYGSLSQPPDAPVTNTYVNRDADYLMMRQMVEAALAAGQSRPATAGPAIAAASAAAAAAAAGRPLLPATPPARRGATGRGVSSAGNRKKSSKKSAKKIKLPPAPPTLVPRPAVPIYLQHLVRPGSASVGALMGNHQPAGIDAAIINAVLTGQAVDPVQGLSPPPGTVNTGNSTAGSPTRALSPVRPLSPVRLPQPSQATRSTGAKPGSESASRPASPVRPGRGPRAASGDDSAGGSAPGSNGGVTAANSGRSDSNSKPRSRNPSPPNRTNGGGGAALQPTRPLSSSNSPGFARHTSMHGSSGSRPYTAPGNGNWAANRLERLRGAAATKAAATASMSPLRASLAAPISGLKARRQAKPLTEVEFGVFNRTMDAALMPLTRAAAKLGTYGESLPPPNIYATLSTFSGGNKSPAAATAAAATSTPLPAVSGSASVHPSLGANADADGSRAPSTSRSSAMVAAAALASTSQAASGAGTLSPTPALPASDGSGPVSSVGTGPEEPARRKLKRRNLGGWDWPPGQGMSPAFDVLEETRHAMAAVLAGAEVDENGRTGPCDRVGEVGRLMRRFQKQETRTSFNLSHAVNGVDQIVALVDVLGHPDLAWVSELLLAGNALHDEAFTPLGARLASAECGALRRLDLGYNNLTAASVELLLPLMKDEVAAMSKAQRLRRGLDAGPLHGVLHRLILDGNPIGDAGAELLCDAASSDYTYLAELRLSRCGLGERGAAAAGRLLENSNAIKILDLSWNTLGKRGGQSIGEGLRNANSIQQLYVAWTGITDIGASHIAKALKGNTTLQVLDMSGDSVSGDTSLVLLDSLAENGALTQLVLRDNPVGVVAARKLLKALHQGVIESVDLLGCSFGGMGGSTVVWDPHDPDGVYDLDLETPAHYQVAVELVGLAAHMGLRSWRAVTLNGKPFRLNNTNQMQLPVRGRLHVEFVSCTPLVRDEGPLTDTEVRSMWFTIADPEGVRTPQGPMTLISPQEEEAAAQAGSSPGSPSRRGGKGGSAAAALPRKSPRGGPGSVSIKSLAAVAAAGRGGAGRGGVPGTTEISSATELKGATDEWKLQLLEALVTDVYLTCAQLKYILRCFDGRDMRTEAVVQAFGVLVDTENFWAVLNELEPSSREQVETRLGKVRMFFPENPTGRYTLMLSQLPHQCVAKQLLQQYIQQYDNKLTHHPNRVCFTACNMDGVPTDVSDPHKLYLPQEGILELCFVDLRLPPPGTRSLSRAQFAILISHLLNQEVLDPVGLTRIIDHPALPSAVGALRRWESWTRKHPVSASVPYMWKAAHEIRRRDDPETAAAMEAAAVAAALEAAQTVRRDTVGVAVAGGAAPSAGSPAQGEGTEPPATPSSGTRGTPRLPRESSSYAPPQTPNSTTAPDTPTRRFANGVRDGVLGPMDSPIQAGGDPPLSQRSQQGVRPSGRPRSGTSGPPPMYVRDMAHYVEAIRILSVRHYLTCWQLMQLLQLLPPSATDERVETVTAFWARVTDRDGHWVDVMRTLTNEQQVRAGQRLGYMNVYSETRPAMHYRLRMYKEDEHELAWQLYNKSLDSPLPCWKNLTIDDQPKRVNQGVNMWTVLRGNAADGTTPQTTLEFDFVWPDEDAQRSLAVQQIQRAWRLFKNPRSGGAAVVPAARTGAVQQPRFVAFNKKKKTTATSKMNDPAALKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAK6</accession>
    <name>A0ABQ5SAK6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000067">
        <property type="protein sequence ID" value="GLI66962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="134" mass="14506" checksum="EEE4EC34A14425BB" modified="2025-10-08" version="1" fragment="single">RCIWGNLQCVLATDAGALEQSAMCAVGKNTRRCIWGNLQCVLATDAGALEQSAMCAVGKNTRRCIWGNLQCVLATDAARGPRHILPCFPRAVEGGVRVLAHISRALVLELVLVLDAALRRGWQWMATTTRMATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAT5</accession>
    <name>A0ABQ5SAT5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011033</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000067">
        <property type="protein sequence ID" value="GLI66961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="392" mass="43608" checksum="697C56FBB2750379" modified="2025-10-08" version="1" fragment="single">MVARAGSQHTAVEITRLVKLVEYLRQHDAQDSNQTSHCQRIQEWLHNIARQLKKSYPRAPPPAANPISGSAGMEESVVQHNSLPDKSEVLAFVHGLQQSASDALKHDLSSAKCVRLEAAIAIMHALVAVLVSGLYLPPLRMSALSTLMVPGSTSCCNKDCRQRGCRGNNLTQVVRRPKVQHPAMEDDPSSSGPSCSTGPPNNVAGVACMQLELNHHKTARFTRNREPLTVILPPDLSKLIQTYVRHARPVLMQRAPVDMTSSQQLLIDKNGQPFNNGFAMEWRDIEERYGAPWEHIPPQKLRHIHSTTAYTSLVQEVARQLPDLQPHAQVMGNSLRVWDSHYIQRPGSIIAQAGVDNLNKWRKAQLGLNNQVLRKKMVERMLDVEAKGPANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAX0</accession>
    <name>A0ABQ5SAX0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Activating molecule in BECN1-regulated autophagy protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052596">
        <property type="entry name" value="AMBRA1_autophagy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22874">
        <property type="entry name" value="ACTIVATING MOLECULE IN BECN1-REGULATED AUTOPHAGY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22874:SF1">
        <property type="entry name" value="ACTIVATING MOLECULE IN BECN1-REGULATED AUTOPHAGY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="91"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="368"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="709"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="771"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="887"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="955"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1432"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="304"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="368"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="733"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="791"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="813"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="842"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1020"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1075"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1439"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1452"/>
            <end position="1466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1473" mass="152427" checksum="38030AC4FDF801BB" modified="2025-10-08" version="1">MKNIASLLLCRELNQSPSGRFNRGFEQSAVSRWAEEDFLQHSSSEYVEFRPPPRSSIAAAFSLDGRYLASTHGDHTVKVIDVRTGRLIHSLAGHRRTPWVVRFSPVDPDVMASGSLDSTAAIWQVSTGQMLNKYDFGKPIASLAFDPSGTMLVVSAGHKVFSWPFTRLAPPVKILKTRRSLRALHFHPTAPHILLTAEVTERRAGTTADGVGTAAAMAAATPPPQQAHGNNRNLNRFQQQQQRQQQAPVHSQLAWIHQAGGVGAGEGVDGGGDLEHVAPDDPDPQEAVDDLAVFIAAHNSMTPSQLQQQRQQSQQDVAQDQQAAAVGPRASAARNAHHQGAAPWPTGLLRNLLMGVTGIFTGSLGGGGAAGAGGGSAAGRGGTVNQQAADGARGVRGTGSSPTHGGHAAVAAEGGGPYVVTGAAFAGGGGAPSVNPGDGGMAWGNAHPGHSAPPGNAVATAIAPPVGGRLNVLAPAHIIRHHNMGGGIRHAIPLVAPPATAPLQSRALRGGTGRRLRDPHVAAYDLMWGWDHTQRMSESEIVRLRELALRAAPDLPSAANHDTPCIVTVRLWDFRDPTRELRKELLVLPRVVLCSEMGIHFTTCGRYMVCCVVRERPSEACLNWQAVQAAGAAQGVAATRTHAHPRVGSGAVDEEGQGGNAYCGCAGNLGEADCGAAGAGVGDMDDVEATHVAVGVAVGPTVTVAPAAGSPGSCRSVEMTDAEARPRGPPLHDGGGGGEQTVGWLGGDGGGETQEYDDEYLYDTATQAFGQEPRVNNGSGAAESAGWRGPHSGGSDGGNAGAGSPAISALSLQQRYQQQGQQQRFQEVHVRQQQHRHNGQPHTEDVEEARTEVSRPGAAAWEMEYDSATVAAYRKMASAASAISGVARGCGRSGGGAEEADGEGGEVRPHSTIRPSISLTHLGGAGLGSHLHHQQHPLHLVQSVNAVANKLSAHDPQISRGGAGSAAAPGAMGGDLLGGDAVQNRSRIPHETQRPEQQQPIRNTQRRHHQHQHQYDQQHRQQPIRWRQQQQHHHHQQQQQQPAHIPGRGVDRQPWPEGPRGHGGLSRTGWPPAGPATAAANAAPHGGGDSDAGPVHEGDAATASVGLPPAVQETPSTFASIKAHLALQRSRDGAATAGGMYGRGVSRDAGREGRTVAGPEAQHVAATTAPAAGVPTATTAATFGVPAAAAVGAGATQRNDAPTVAPGIAPVAGGPTATAAAVAAAQPSGGFRPCYELCIFFTGTSPIPPAIVAPADPAIATATVTTTTAAAAAAPSASSSVAFPMAPGLAGTGAIPLAAGGGSGAVAPSTTSVIGPHFGALLHARPVLAAHCMTSVQLSPAGDHVLVAYGRRHISLCSLVACMGQLAVVHSVVEIYSVPDLVLRRVLLSADDEVNVAVFNAFPAEGMAYGTKEGRLRLVRYDRRPPVRSAIPFLPTPPHLEDETWEAEHPEFQQQDSGKSMSVSDGEQQRPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBX6</accession>
    <name>A0ABQ5SBX6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000068">
        <property type="protein sequence ID" value="GLI66968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="365" mass="38947" checksum="0285A7ED97E23E38" modified="2025-10-08" version="1" fragment="single">NIYVQDLGAMSLVLARNEADASGRNYLIIKDVANQLRNLGAPITDRLLNSWVDARKSLKRIIVKDPILLGRLKDLQVVGRRASAIKLASCAAICRAIVNVAILQPLCLAIEELKKAHPPLHATCAQVTPVQLKRAEVYEARLPAAKRLCTISSAAAEGARDEAEAEKDNQPVPNKTSQQEDHCYSQNPITKITNSSREHGTCDEPADAPNNIPIIKKAQQVIATDEQRQQQQQQPPTAAAAFAPSAANAPAAATPADAAPSISTPHQEPPVCKLAASDSICTKPVSPTPIPRTFPAAPAGHRFSRRPLISRCIPPVMSTKTFHQQAGPIALAGGPKTSPPPPRLLPPPPAAAASRYYPPLTLLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCQ6</accession>
    <name>A0ABQ5SCQ6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011603</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02980">
        <property type="entry name" value="TRX_Fd_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47682">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47682:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="107"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="231" mass="25373" checksum="66024B138B3E8DD0" modified="2025-10-08" version="1">MVLRYAVSRRRVGLSVQCQAKQYELRICVNRTCKRQGSEQLLKFAVDLGLPSLTVAPIGCLGNCGNGPNMVVLPDEQLLHHVATPNDLAQVLRAFCRTSIDDTILQATQLRLAGNAHAAQQDFRQAINCYSQALQLGPSVGLHMLYSNRSAAYLQAGDKDAALADAQRAVEYAPPGFHNAAIRLIDTLFALGRFDEAAELCKRTADQDSSFKFREEYTVIKKALQSVGHKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCT5</accession>
    <name>A0ABQ5SCT5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011473</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="185" mass="19301" checksum="CB82EB7BEB5F142B" modified="2025-10-08" version="1" fragment="single">MLSEQTVAVRDCGFHGASSQGSGPQTLHALKATSSTARSCESGATALRSPYPHLQPIQQHSCVILYNPFLQVANPAFHYQDAATSMDSSALGFASTPDLPIFSSATSSPVQVISPCSPSPSTSLTQSLKEAPIVLMPRTAGKLGLPKLLPLTDAPEGSILSPQWIPAAVTRQGTLRAAVRGLLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCU4</accession>
    <name>A0ABQ5SCU4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">snRNA transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_011995</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNAPC3/SRD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022042">
        <property type="entry name" value="snRNA-activating_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13421">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13421:SF16">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12251">
        <property type="entry name" value="SNAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="149"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="252"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="389"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="637"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="742"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="397"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="759"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1103" mass="115079" checksum="246AB550394586FC" modified="2025-10-08" version="1">MATGRGSSTARAAPTPEVQNNAPRRPSLALQPFPWGGLQILPVAPTATELVAVVPPPLPGLHRTAPFRVSEYDERCRIAVMALEAALGPGELADAEVASPWATGGLDLDLDEDLRTVLDLSDLAVPLPPSISRACGNSIGISPGCETERVPPNLRPSGSPCGRIAAGTRTEGTATAAEPDHPAEEMRTPPVLPPPQSAGEAVPAASTDKLEEKSGGFKQESSSGGSPTVSNVMAAAVAGSCGEHQGARRVAGASCQNGAGPQRQDVLGRGRVPEAGPGCEGGLSHQQGGAVAHGPYASPSEISNLKATGPAQLPAHGHRRPPVEHETQGPQKLFQQPLENHQPMAQDDGERPAKRRRAAPAWLRKDYQLSPLQLSPQEVAVAAIAAGAGEGKTGDQQPRHEPRTQRAERGTGKCVKAKGHPQQPLGNGTAAVKDDVQNPGPPSCPSGRVGGVGGNGKRASEVGAGGPQQQLQDEEEEKEKQALAWWDSLLAAAPQLESLKRALQAVLLQPPPRGGANGKRQGEDQMQNAGESSKDGIVDAEERELQEEVERGERARQRWLAGEYVDLHVDEGPTGARQRQHATVAGTDHPVTLTCGAYGPAIEAAGSSGWQRCLPAAAAAALERIQLAVNGPPQLPPLTVGLRGGRPGSEDPAHDQQLQGGAEPVDPSLKDEVVLWVVVCGPGQPHVLHEEYLVLGSQCLSDLRDVLGCATEDAMRQAAQVAQVPMPGTAAACDEDGGCGAAGKGRARNRGGSNGGSGRPLEAAAAGEAATSPSGGPGGPTSRRRGRGRGRAEARLPPLNSAVRAGHSDDGAVTAAAAAAVAGGDENRNAGNPAALAGSPPLPLGSSVQLLPLSYGSYLFLEGHFYTDVRHPDAEDYTAPIRELCSAHGVRPTYLRPERSWRPALVRHDGGTLPPPKPMHTTRFSELTLRQANHPTGIFCHRACCEHLMFMRDVRRWHPGDPADRSAFPVRLRTRDRAGAPVTRRRCSVCEARYAELVTHDDPLAPSNPAVMCRHCFDLLHFGSNGERLAPNVIAQPYNPALDIATQGIWAPSAGAWTALNAPGDTSGGGGGCHNDNSARIWAGGRVADGAGRSGAVRKGPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCU6</accession>
    <name>A0ABQ5SCU6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011652</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="359"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="563"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="625"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="824"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1003"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1083"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="718"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="80"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="287"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="376"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="484"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="509"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="575"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="843"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1003"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1029"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1171"/>
            <end position="1184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1246" mass="126448" checksum="11CD6C02BBFC8C60" modified="2025-10-08" version="1">MEYAEYDTEVRFAFRSYRSSYDEPDSPPRSRTDPDELRDGDVDSEPGILGTLTSLLVGGVTELLTDFSPHVTNPPVERYASSTSSTSNATNTNTTVTSTSSPISASSSSRVANAHRRGSRPRSPKPSHGPRGDAGWNSLVSPGSGSRQRASPTASAAPLSSSIASGIGGKAASNRNTSSRDGGDEGEDSGWMIPPQRRSQPMATPAAVTRSRESDNGDMAPANSLMGSPTAGGSSGGLLGAMFSGISSLGQRATDILTQLDHDFETIFNGALEEEQENTFIGADSDNDPRVRPVDQSHRRAQSNSGSATGTGIGTSHQDDGRSGVISALGKDSSWSGTLQAASGGAGISAAQKGPVGGWTVGAEGASTAGKPPLVGRQSTARSAGGSAAASGGRGGSAAAGRSRQQGTGRFAGGSGGDFWNKQSDDDDNWGWGSSESETGPIAGSSEVAVVVARQSPATTRTQQEPVAANTASMSPPAGLQSLVVPGTSAVSSAATPSSSLPRNGRNVGGAASVSTSAASLTSPSSVRSSRSSGGKLFIKKTCATAASANAAADRASTAASLGNSATSMRPMDASSSTVTHGSHSVSVTTSPKKAAAKGAEPAPAAVGTAVGSVKSAVTLAAASAKTESHTTTSGSQVPPPGPKQVLAGSPKPRLQVRQTQPTLEHPGASMAAAAAAAETKAVAAAAAATSAAADGAAFLAEAKAAAAAMAASAASTQRDLRQQLDEARRCADAAEARCTELQQQLEAAEAETARLKSLTEVMQTQLEASQLRFEALIAEHTHLRATNTAAAVAATHPHAHHNPHTHHRHHPVYLADAACSPTRTGSPAAALSPPPGWAPGQGPAGRQGSGGNSRRPSISGGGSDELVEATLVAQLTAALADKARLWRENDILTRQLEGLQELLTYTAQQASYAHDGDGCSADDDDHYIMDAAAHDTYDTPYYTSHSGATMLSTGSPLPSVVLETAAEPTLAAGAGLSLKSPISYESAGAFIDLLYDMGDGGRKSGGDKGGPSERGAEERQELPYDGVGARGDGDNAGGGSTAGDLVHTGDPAARGRLSVACDASTLLAGVCSAAGVPDAVPDADADADASGATQMSSESPGCGSQRPERSFSAAIPHSQEAQPRRPAWAPNHQNQQLQKGDMQEARLGQMEMDQQRAGQQQLPPLPLPLEAPLLLPPLQQQDEQQNELTSPVAKPPVRTGSYPDGWEVLDEQGEVVGPQQQGGGGSEGSGDAVRIATVRSGGVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD23</accession>
    <name>A0ABQ5SD23_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipase A2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012115</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047479797" description="Phospholipase A2" evidence="3">
        <location>
            <begin position="21"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15652" checksum="5A2E10748720D61B" modified="2025-10-08" version="1">MLISLLVTFVLLTFGTSTRAVDAPGAEETPCARRCHTINCDNMNIRYGRFCGVGHGGCPGVKPCDPVDRCCQKHDACVAKHSVFKAECHTSFLKCLDRHVGKDHEGFAPDKCPYSIVIPTMKTGIEMAMMFTSNFQGEASSEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD88</accession>
    <name>A0ABQ5SD88_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lon N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46732">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE LA (LON) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46732:SF5">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE LA (LON) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="41380" checksum="AB077A98757E246C" modified="2025-10-08" version="1" fragment="single">RQRVVVYQAVRFPAALPLLPFTPKEVLAPGSCKVLRLYDSSSYMLLLEELLSSPHQMLIHSVVEPLESNTGTLSAANRLGAYEAGGFVFCLSTIVKVIDVKQTESGVLLRVQAEGRVAVKSLAQTRPYFRAVVVPVTDSVDLDRLQTPLWRDICASVERLRGLMKDVQNLAAKFKSPETANLQRAMYWVDNPHPLVFSSSSMLLNSRTADGGRNTASVGPLASYDDFQPSKILANQVLPDTEPMYTDSENISSGSDSEVDAQYAAAAAAGAEPPPPPFAQLPGAAAGGLAAAAVLGGGAGSILGSGAIRTRPSLLDLERACRLSLAAIQMLPRATDEEREKIRRHQVDALETQDVLERLQLAQRVMGEARALLSAKCAIMALSTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDK5</accession>
    <name>A0ABQ5SDK5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BACK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BACK" evidence="2">
        <location>
            <begin position="313"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="647" mass="69891" checksum="A3BB8889F78313E9" modified="2025-10-08" version="1">MTGSVNKHVARAISSLFASGRDADMLIIFCQETGLNERAGKDDSRRLVLSDPLPAHQLVLRYASERFAVEFERWGKQRPDSLGTQVVPGDVSSIDYDWLTSPRSDALPADDTLASPDSTHVPQRQSLQPAQITAADPGSESPAGPDLLQSPATAHKGLPISFVPLGSADEVPSARAAIHFAYTGRITAGSIREVLELRRQGAYLQITGCTAACDEAIISMLNSGCTPRNNNGCDGGNGSYSSSLSNQGCNGQQSATSDDTGGCGLPAGVDLYINYSLWPDLDADLGFSSVLSAAKPQLVAHFGDALKALNTSLLREQLLVLPAVALEALLESDEFGTDTESTVLLLLAVWMEANFILTNSDMRQRLCRTIRLAHLSRPYLSLVLPALAADHEKDPGSLPGWFPFDVMQAAFVANLAGLPESERMELLFLSSLPAYVFEALSLPRRRLCSTASGLTFSWHAAEQELLQKNAHAAQPGCISSVPCIFSEDITRCAAHGFEWRLCVPQVAEDMNTAISLFCELPAALAFPGSRLSKPCGLWTHVPVVATIKVQAGTGQGGVQIKKGGRSDMLWVGMRWDVVLPSLAQRQTQHQQQLRQRPQLRGLLRHRHRQQQQAVDQSVAEDRHLLAAWTGYMLSGKVSGTLTLQGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDU6</accession>
    <name>A0ABQ5SDU6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15852">
        <property type="entry name" value="PLASTID TRANSCRIPTIONALLY ACTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15852:SF54">
        <property type="entry name" value="PROTEIN SSUH2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="170" mass="18522" checksum="1F5A680CDBEDBCB3" modified="2025-10-08" version="1">MMLQAQKPLWCSHERPRRSMLASKKRHIVIVNFKPSSPTSRSPSSVGNNANYVEGVFQKSIDVMLNRAASSLKRGTCTCMSCRGSGTVECAHCKGSGRLSTQAAERAGLLRGALLRLRTAMGLPPHEVPYASSGWMRTNRCPRCHGVGRLACEPCTGSGQRFPHQPGRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDY2</accession>
    <name>A0ABQ5SDY2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Guanine nucleotide-binding protein subunit beta-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011909</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="610"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="638"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="114"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="368"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="760" mass="76986" checksum="0A5A908DF93E9388" modified="2025-10-08" version="1">MGVFSIPPIPVGPGSCRLFLELDKHEARATIDRLKLMFERLVTGKPAVQQIVTAPLHTHAVPENLPPTSAVEPPPAPRPSLTSAQHMLPLSQQQVRLVPVAAPTAPVVLAPAVSPAPAPAPAAAPTAVTAPAPAPAPLPPAPQQPMQAVQRPMSTTPFPYSPAVHQPQAVPSQAPMVASQPAPPAMLRPQAAPFMPSGSATAGAAAPVGRTPSSSAGMLQMPARPIIGHSQQQQQQPLVARVAPLPLGGAQAELPAVHTAASQHTAAPAVQPAVVPTALPQPAALAVPGVALGSAVPAAGGLRSAPVPSMYQPSTLSATTSGAAGAASRPAAPSPAVLRPAAPAARLAAAAAAAAAAAVKPAAAALAPVAPTSRPTPAAVTASSGPRSAPRASAVASGPVWGSCLVTDAVDHNRDPVPALQYCPVLEGLPGGDGHGGWLISSSRRMLNLWECSAGGGRGTPSLMLLHSTETDFVATHLAAESSSRVLFASSTDHRTGAECVAVHSLDPEAGMLSLRYKLAVTNPNTQRPTPPPGALPPPRMLSKVASLNAFGGGLRGTCAAAYQGTVMVFDAPGIFGPPGGSPSGLPPKGSWKAHENTAITALGTSLQGGARLITGSRDGAVVIWDMRVRPPGNSWRCRSHQQSVTGLQLPNETTLITCSLDGKVNIWDLRSTNGPRNTATPDGSAVLGVKTSPAGDCVAIHTQRGLLTVDLLDSSASVVPVTTAPLTRPYLDIVWNMSTAEIYAATATGSVTVYRQSYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE30</accession>
    <name>A0ABQ5SE30_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="501" mass="51419" checksum="B61CF43BA7CB94A7" modified="2025-10-08" version="1">MGQCLGKSQAQSSVKDPFPDTCTESQICNYFVDRLPARVLSDGIHLLRGGPGPIMQGWTHELLNDAGVHSYQDRELLLKTRDEIVDAVEAHRQRLRQLHPSRSASCADCQSRTASLSPEADILCNGTTPGSASRAPDSPKTLKSSGVSGSTSVWESSAAESGYSLDKATARKQEEEAAATVAAEATAKATAFTSDGDGKSFRLTTDWLPAGLRLYRISEEIRHMASSGTLDVSVHSKAAGVLDGPAAAKVVAAAAAAGGGAALLQTRSKSRFYLSPGGRPGGGGPGGDGIQSETDGERNDGGCLQDRDGDVEAVTLTAVVAAAAAGAGSDAKVGTGATSTPDSSPSGASLLATGPSLGTAIQLSPRKHYFEKGLTGTAAAAVETGGGGRCESISRSREDSGSGEGSGSPGSTLGAESLTALTILEAPPPPSAPPQSQLVDSYPSDAECAVAGHDTRPLSPCVMEGDKSVNSTALDSEKRRQPDQQQQQQHKQQQRLLISVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF18</accession>
    <name>A0ABQ5SF18_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012598</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF11">
        <property type="entry name" value="METHYLTRANSFERASE TARBP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="4">
        <location>
            <begin position="426"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="313"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="578" mass="61643" checksum="72B9AECE463C3A18" modified="2025-10-08" version="1" fragment="single">GSAGGAARAVWQCMEQPHHHSNLRPLMQQALLALLDRWPEGVQSLLLPALRTYHTASYQVCVSVVIIGSLLLQRQMASQPAGPLAQQLLEALFPWALHHRHAVRVPVQTVLYLVLRKQARGVTEQTTPLTRPPLGTEPLAVNSSSQMPYGTANTAYKELPRQPLNLPAQLSLGLLDGVRVFLGNNPEQVALRTRQALVDFERFNLEFECSLEGLFGIREEVLMAAAAAGEALEVDPGYPSIWALHTRDRLPIDVLRALPAVIRSLGASQKKAPAAGGFRDTATAAAGPTDATAAATPAAVPGLTAGLRRNTGLNALEGETEEEEGSQEEEEEEEEEGSGVGEQGDVDEEMFGGEVAEDVRRLSTAAAAVGTLSYQLRPQLLGAGAASCTGRGDAGRFMSAVTAAAAERLDALAAAAVPQCSRPTGLVVVASLLENVPNMAGLCRTCESLGCEALVLPSRAVVSSEAFKRQAVTSERWLPLLEVGPRDLPAYLAEMRGRGYWLVGVEQAAGSVPLQAFNFPTRCVLLLGNEQSGVPQHLISGLDACVEIPMLGITRSLNAHVSGALAVWQYVQQQQRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFT0</accession>
    <name>A0ABQ5SFT0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_012541</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004696">
        <property type="entry name" value="Tpt_PEP_transl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00817">
        <property type="entry name" value="tpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="10">
        <location>
            <begin position="87"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI68197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="390" mass="41427" checksum="6F3800D402AFAB6E" modified="2025-10-08" version="1">MSASALSSPTVNASLSCRNPIPVCPRLQNCRIGGVLISRTGARMPSTTYPGAASICTRESRTLKVAAAAASESLPEGGQSEFVSTLVLGSMFAGWYAANIAFNLYNKQVLKLFAYPVTVTEVQFLVGSVITLASWATGVLKAPKITAATIRNVLPLAVVHTLGNLLTNMSLGAVAVSFTHTIKAMEPFFSVILSAIFLGDHPSPAVLMTLLPIVGGVAIASMTESSFNWFGFLSAMGSNLTFQSRNVLSKKLMLKKGDGSLDNISLFCCITLASAVLLLPFSLLLEGWKLTPSSLAAMGVKDPNQLLKWAVMSGLCFHAYQQVSYMILQRVSPVTHSIGNCVKRVVVIATSVLFFRNPVSLQNALGTAVALAGVFAYGQVKRQANKKKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFY5</accession>
    <name>A0ABQ5SFY5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="117" mass="12510" checksum="22633FF705BF0C0C" modified="2025-10-08" version="1" fragment="single">MSHKLSQQNFNTQLKALLSCFGKLPTENNLPGTLSTSEGNGPSICTSAYNSTAAFAAEAKKAPSDIEQPNPPANDVHCSHSPLESRFEASSYPAIISKPRGAHEGYLAGVSEQSYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG47</accession>
    <name>A0ABQ5SG47_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="646"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="660"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="758" mass="77537" checksum="CF8D3BC4B8E45422" modified="2025-10-08" version="1">MDLGFAYKIMRARAPVGYPPAMTTSPGEPAAPAPTTSIPGAAVEQSSEPQTPTSRPRRSLSAVALTWAPVLVEERSLEVLRKPSAVDSSGAPAASGSTAAAAEAPSPAPTSSPPAILGPLHLVPRPLVPSARGVTIGPFAEALAAVAARPPRRRPGADASALRLLVGGGSSSSPASPAVSPQPPLSLSPASPPISPPLRNRAFINPNYQHQQHHNHQQQQQQYQQQVQGAVQGASAQSNTTAAAPAPGGSKRARRGVVQQQQHQQQQLQHHQQQQLLLLPRVASQKAVHLRQQQQGSHSPTAAAAVVTGAAALSITSLMPSPSAPALSLAMAPALEVEASGQGVHPSTSPVSKESSTAWGPRCARNTTTGALQGLVAAGPITTIGHAVSTRPAAAGDAVEAAVTLPPTAGAQGSEEWECRLGVPSAESPGLISGEADSGGGGAAAVAVAREVEAGRHGEPSHGATDDGTGGHSHHDGVTASGGGAGGNGGVNGCTREAATVQLPTLPMLAMGQSYMGYQGSGSAVSRGRTPRQISSVSATSASQGGQTHGAHRQQAALQGSGSWRQMMAAEDAAGMLWVAAGAGGGAGGGHLVIDPEAEADAAELDRLEQWVAASQRNPAMVEVAIEQRRALKRLEQLRSRLLRGRGASSTDGICESDGPEPGSGTTHSGSGSSMIPSWVADAALTGRIGSDSLVALASQVSRLPHIDYDQMRDPQYRMKLKARSKQGRLAVLNPVINDELEDMPIRGRRRPWKPLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGS3</accession>
    <name>A0ABQ5SGS3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="110" mass="11405" checksum="DC460E04B0415410" modified="2025-10-08" version="1" fragment="single">GSRAIRLVGVIDKPRRLSEMLISHARATHGSPRLTAAWRSLADVHRFLCHSEPKQALEGPYRLRCPDTSPLVLQSAGTRRGSVRQGPDVGGSLRLGGGGGGGGADGGADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGX7</accession>
    <name>A0ABQ5SGX7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="756"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="881"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="710"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="723"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="771"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="950" mass="100787" checksum="7FCC2878EE905302" modified="2025-10-08" version="1">MNWGLGGQYTARVGIKQALSHVYQVWCPHVAQGPRLGAKQAQATSLAAPSYAPGGGLPIGHALLPDDSDCDSDCSPCAPHVQPSSTSSENPVYVAEQCDATSDDPGVATRPAVTWLLDHGDSGTLAKCASEASSQPSSSGAWRSRQHAAPAHVPISSPKSAFVYRRPPADWTAEASSSSSDGGSIRASASRSCSSSIGRGSVACKDAKSNRHSRTQAYDGLRRSRTRSQSTTIDGNVGLRCTSDMDGAATCTTGGQESAGLTAVIGADPPTISEITRISARGPRLAAKPAEGNAAAPNSTADAATCQPVTLERLRERTPELAVLELSTLQQRLEALGNLLQLDDTSSLMLCRVVPGLLLGEPGTLRRAWGRLCVALGGEEPTRGACVAAPLLLVLPPDRVPSCIASLAAASAMPRTQAASLVAEWPQLATHAPASLAAKLEGLQKLLGLGAADAGPHEGGVRARTVIRAALRDAPRLLTFSTSALVRHHAELSALLGPDRLAAALRREPGLLLLRPATVATKLALLQQLMDCAEIPGAVAALVVRQPGVLRRSLAALSRSCRALSIWKLRRRDKLRMCLSRPGLLTLPPTEVHGRCRWLRRLMLSNAYYHAALRRLPPSLVAVVVATLPQAWTRLQYLAESSQECRMHVLDAVECRQEDFAVRFPEFTRWLQFKIKLMGAENPWRGTLRIRGFNTARDKPAYGSANQLNLRRRQQQRQRSSRRSQQCQLQQQQHSATEPFESVVLAEACEVRSGGSTVARPRRVLRQPMDAVPVPFESSSESSSEASSSSEAPSVGAPSSALGQDGKGLPNASPALVILPQIGVEVGAVPLSSAAQRRRLSGTTSDTAAAAVGTAGLAVSAEAAGAATVELNRYRRLCRPSLGAGSGQPPARNGVTTAWPGPRGGKSDPVGCSRSRERRGGLKRRGASTFRVVQVFSGRNRYEYQWPPDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH06</accession>
    <name>A0ABQ5SH06_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="214" mass="23090" checksum="A4B467DC21909343" modified="2025-10-08" version="1" fragment="single">KTYNKIPKTRIKYNLSIVYTRELFNSVANVSKSRCILLIISQMASIKNKVHIIVGAVLALIGWLVALGGIAKINRDCNDLYETVVKWCSDALSYQWWGIWFAFFLAVVILVLAILGKAQAWGSTLQALCAACLSVTMMDANIMYDYSTRGSTIDAVNAALAGFIILSIALMFLIITLGMGAAGVNVSVSVTPAPGRLETVSTQPKQGDTAAFAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHB4</accession>
    <name>A0ABQ5SHB4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-L-isoaspartate(D-aspartate) O-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.77</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013189</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="287" mass="29895" checksum="430C1CAB9F83F436" modified="2025-10-08" version="1">MLVAGIRIFSGRPLLARQIVTSGGLCNAAATVNFENDTQDTLVDSLVKQGLVQSPRVEAVLRALDRRDFTFTHMGIPSHVSYRDSQVPIGQGQTISAPSLHAICLELLATHVAPGAVVLDVGAGSGYLTGCLSLLAGLEGRVVALERHERLVEQAWRGLIRGLVPILGDVGRSQGGGTGAAALGLSPLRVHNIELFHGNALEERALAAVGAGGPFDAIHVGATAREVPASLTELLRPMGRLVLPVGPPVGMQSLTVVDKAADGSLSSRQVRDVKLAPLAPPNAEKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHL9</accession>
    <name>A0ABQ5SHL9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U3 small nucleolar RNA-associated protein 15 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013832</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018983">
        <property type="entry name" value="U3_snoRNA-assocProt_15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924:SF26">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924">
        <property type="entry name" value="UTP15 U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 15 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09384">
        <property type="entry name" value="UTP15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="123"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="165"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 15 C-terminal" evidence="7">
        <location>
            <begin position="371"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="521" mass="56972" checksum="8201E5E70B3D700E" modified="2025-10-08" version="1">MDHELADYQKLQIRQYAPRSIRETAEGKYWRHFKAPIVSKQFGAVSHIDFCQQYPFDLAVTASTRVILYSGRAPNVVSRTITRFKDVAYSGCFRLDGRLVVAGGQDGIVQVFDANSRSVLRQFKAHKRSTRVARFGADRLHVLSGSDDVTARWWDVSSGTQVLRLDGHRDYVRAAAGSPNSPDVWATGSYDHCVKLWDLRAGNSAILRLDHGAPVEDVAFFPTGALAVTAGGNYLCVWDVLGGCRLLKRLDNFQKTVTCIRLSQLAGPESAAAPRMLAGSLDGHVKIFELDSFRVTHASKYPAPVLSLGLSPDCRLLAVGLADGTLSVRKHARPKVVPGAPGLPQPKPKYQRQLTAGTYRYFIRGQSSRAAATDFRVFKQRQARLQPYDKLLRKFRYGAALDASLSTRQPEVVASLLEELAARGGLAAALAGRDAEGLRPLLRHLVKYIGEPRYTKLLSNVGHRLLDTYAEVVGASPAVDQLWRALHDRVAEELGVMGQLAQLAGALEPLLAASLAGGPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHS4</accession>
    <name>A0ABQ5SHS4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP adenylyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009163">
        <property type="entry name" value="Ap4A_phos1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043171">
        <property type="entry name" value="Ap4A_phos1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045759">
        <property type="entry name" value="Ap4A_phos1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019200">
        <property type="entry name" value="ATP_adenylylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38420">
        <property type="entry name" value="AP-4-A PHOSPHORYLASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38420:SF1">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_5G14690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19327">
        <property type="entry name" value="Ap4A_phos_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09830">
        <property type="entry name" value="ATP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ap4A phosphorylase 1/2 N-terminal" evidence="2">
        <location>
            <begin position="129"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="ATP adenylyltransferase C-terminal" evidence="1">
        <location>
            <begin position="328"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="47836" checksum="A52435F3319232D8" modified="2025-10-08" version="1" fragment="single">MIPARALRCQRTYNLQIKAGSTSTWVTTTQERPIEQIGACRSCSNLTGASTSSSSRTSKGCGYSSVLLRGGAGGSSVHRYCQAGGVVRPSIITMASSAAYADVAGVGATSVEATATATAATATATTAAAAPSLWDDIVSVYNKAQVVGACSKTDTQVEVLRDPSLGIDFVLRLATALKAKPQGPPASQPAPGPTAASSVSLWRNPFLPPEQELFVRELDAEHSLVLNKFNVVQHHVLVISREFRSQAEPLYGGDLAATLEVVRAMPSGGVAFYNCGPNSGRSQPHKHLQVVPLPFADGQLPLAPVHHLVEEAAAAAAAVPLQPLHLRQLPYRCYVALLPPKSTTPEQLESAFSELLQLCYPGYVFQPSPLLSKGPPRDAATAAAVVAGSVSYNVLLTRSWMMLVPRSAERCGPLALNSLAFAGTMLVRSDDELDYVRQTGPVEILRRIGEPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIK5</accession>
    <name>A0ABQ5SIK5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">microtubule-severing ATPase</fullName>
            <ecNumber evidence="7">5.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_014084</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.</text>
            <dbReference type="EC" id="5.6.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF86">
        <property type="entry name" value="SPASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="412"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="248"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="653" mass="69286" checksum="E1F5C212FAD1A8FA" modified="2025-10-08" version="1">MPSKLSYDIRKNLIYHIEVWHTLLALPIGIASPNDAIDDPPVLASSNLIYTLTHLQLQIFSLAAAVVLSVVLAFGAAKLRARARYALRRITGSTPGRQTWGISILLLDGPSQVYHNLAVRVLRCGLRWPLHHMFESLASIFGGTGRSSKDPSKQQEALLTKNRQKLQEYHKLTQEAIDRARVADKQGRHDVAVRLYGTALEAALEGLNLQVAPGNGLGPKADSVATWRNDLQDWACQVEVRLQAIKSGNAASPSAPPVRATQSSSVTKHPQTAVAVSPKGPMRSLSGGTAAAAAAAAAKRPVSTGRTRPAAAALAGSSSKSIALHFPIRSSAPAGAAQRGGGGGGGGGGAAAGGRDDGLAKYKELVMGEILDRSPSVKWDDIAGLVTAKAALTEAVILPALRPDLFQGLRAPVRGILLYGPPGNGKTMLAKALAAQSQATFFNISASSLTSKWVGDGEKLVRALFELAAERQPSIIFMDELDSLLSARGRSGEGDAARRLLTEFLVQFDGVGGAGRERVVVVGATNRPQELDDAVRRRLTKRIYIPLPDTEGRTAVLTHLLKGQKLRLNDKEVMALVQATRGYSASDLAALCKEAAMAPLRELGPERLASVPASALRPISKSDFEAALRVVRPSVDAASLQAYEDFTKTYGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ33</accession>
    <name>A0ABQ5SJ33_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial</fullName>
            <ecNumber evidence="11">1.14.15.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-methoxy-6-polyprenolphenol 4-hydroxylase</fullName>
            <ecNumber evidence="11">1.14.15.46</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">COQ6</name>
        <name evidence="14" type="ORF">VaNZ11_014617</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">FAD-dependent monooxygenase required for two non-consecutive steps during ubiquinone biosynthesis. Required for the C5-ring hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 4-hydroxy-3-(all-trans-polyprenyl)benzoic acid to 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid. Also acts downstream of coq4, for the C1-hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 2-methoxy-6-(all-trans-polyprenyl)phenol to 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol. The electrons required for the hydroxylation reaction are funneled indirectly to coq6 from NADPH via a ferredoxin/ferredoxin reductase system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)phenol + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81183"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="EC" id="1.14.15.46"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81195"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="1.14.15.45"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a multi-subunit COQ enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.45" evidence="11"/>
    <dbReference type="EC" id="1.14.15.46" evidence="11"/>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03193">
        <property type="entry name" value="COQ6_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018168">
        <property type="entry name" value="Ubi_Hdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051205">
        <property type="entry name" value="UbiH/COQ6_monooxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000689">
        <property type="entry name" value="UbQ_mOase_COQ6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01304">
        <property type="entry name" value="UBIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="FAD-binding" evidence="13">
        <location>
            <begin position="411"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI69910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="537" mass="56354" checksum="8021AB08E1DE9853" modified="2025-10-08" version="1">MRRLSPGIFWSRSKSNEASRALVRCNVPISTTNTNTNTNTNTNTNTNTNTNTNTTTHGVHLTEDIYDAVIVGAGMVGAAVAALLGTNRLTSQLRVAVVDVKPQPTRFRPSAVPDLRVSTVTPGSIELLRRAGAWDDIRPPLAASFANMQVWDSSSRGYIRWDARDTGAEQMGIVVENTLLQAALLAAAERNGRNTELIWPAEIRSLRLPYDGPAAGPSDSTGIDSDGGGEGGGFKPPHGLAELVLGNGRVLATRLVVAADGAASRVRQMAGLRTWGWGYGQRGLVATVSTAEPSETAWQRFLPTGPLALLPVRDGYSNVVWSTTPQTATAMEAMSSAAFAAAVNKALRDTSIPNTPGTLTSLLGPMAAFLERLGPFAEFGRNSGSGSGSGSNGFQDPPMVLGWAGGPPRSFPLQMKQAGRYILPRLALVGDAAHAVHPLAGQGVNLGFGDAGVLVEALAGAVETGADPGDARILQEDYEAPRRRAVLTMVAALDGIKRVFEVQDPGFATLRGVGLSLVNATGPVRNGLMQYAMLGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ50</accession>
    <name>A0ABQ5SJ50_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_014221</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1020.10">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035898">
        <property type="entry name" value="TAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000197">
        <property type="entry name" value="Znf_TAZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808">
        <property type="entry name" value="CBP/P300-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808:SF1">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02135">
        <property type="entry name" value="zf-TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00551">
        <property type="entry name" value="ZnF_TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57933">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50134">
        <property type="entry name" value="ZF_TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TAZ-type" evidence="13">
        <location>
            <begin position="17"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="137"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="246"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="326"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="546"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="620"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="246"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="568"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI69714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="655" mass="70198" checksum="98666594D70B6509" modified="2025-10-08" version="1">MVPRAPVGHMTPLNGDDPAFLETCHRWLLFHYHCKGCKYNENSCQYGKVCSYGKSTFTHVRGCHRGECSFPRCALIKSILRHHADCQDDDCRMCSPVCKYQARSKSAQAHSQAQTMAEISRAIPKGFCVEGSEQLPPLPAQLPSPQQGLRKRDSMNAQPEVQQQGCSSKAVQQQKQQNAGMLFEARHREHKRQFSSSLSQTSWSMDAAGTALTNSSAASSSGCTLTHAPSESLDAIAELQALQLEKPAQQQQRQPEPPVGQEQQPQQHQEQQQQSTADSSQQVPVKGQLPAGQQQLASPPVPPTGSRACCSPLGCISVPSSGGVMDHPAPHQAVGSWPVPHVAGGMPQQLRQSSPPQPPPPPSHHDQQSSSTYGSNALAMLMHPSLLEQQPMSFMPVLQPHNSHPQAILSQHQQLQHITALQAHEQRQAPLSFVREPALGAAMQCATSAGAFQVGLTNPTAHHHHHHHHLHHQPQVFGVVTTAVAAGAAAPQASGAAGAHIFPTHMPLNTWPFMSLPYNTFAQTLQQQPSQLGNMASGMSYTPVHHDPYGSPGNASSVLLPSAYPPYQHSQMPPQPLQQPLQSPQVMTAGSGAHVQGSSSGVFMGSISPGPLAQTSLGCGPEAMDSDGVAPGMPASWGPFRHPGLPLHGAHSRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ64</accession>
    <name>A0ABQ5SJ64_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plastid lipid-associated protein/fibrillin conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014087</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039633">
        <property type="entry name" value="PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006843">
        <property type="entry name" value="PAP/fibrillin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31906">
        <property type="entry name" value="PLASTID-LIPID-ASSOCIATED PROTEIN 4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04755">
        <property type="entry name" value="PAP_fibrillin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0934">Plastid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastid lipid-associated protein/fibrillin conserved" evidence="4">
        <location>
            <begin position="81"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Plastid lipid-associated protein/fibrillin conserved" evidence="4">
        <location>
            <begin position="289"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="333" mass="35742" checksum="835F0040BBD9F8C0" modified="2025-10-08" version="1">MQTSCIVLLRPGLFIDLRGTRWILPRRSPHPSRASGESRSGDTESIPTASPRLPLPPSFQEPRSQQQKSLRPDDNDARAQVKVRLRQLISKVNAAALPGPADLASLGQAVQELIRLNPTPCSATSDLINGRWVLLYTASIATLRQAAATEASASNPRQGVAGNSSNNRSNSFGGSDGYSRALEAALSPLQLANEVAYRFFYTYVPVLAGAAVGSRGGSASGPVKPRGNFQVFNTQQGTVENQARFEIGGRLCCINVNGAATVVEGPPGQPRQRLRATFTSFVVLLDGQRRLSLPLSFLNPQVGYVDTPYLDEEIRISIGDKGSIFIAARENSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJD5</accession>
    <name>A0ABQ5SJD5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045318107" evidence="1">
        <location>
            <begin position="27"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="147" mass="15921" checksum="8367004D548C9FF4" modified="2025-10-08" version="1" fragment="single">MAKLLNLLTVTMWPVVVLTFLCVADAHKGASFGAQAHQQNVGVSGFRLSRLLNIWRPAMLKATSRDLPAVQDNLNLVSTLEAVFELPLAQQPSRCETGLPSGCVVELPLSVPKSTDYIGSWKSAASSGRFVGYRTVPAVRIICSFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJJ2</accession>
    <name>A0ABQ5SJJ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014949</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF289">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE VIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="716"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="22"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="369"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="437"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="520"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="826"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="85"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="520"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="749"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1188" mass="123660" checksum="07677C7BC69598A1" modified="2025-10-08" version="1">MLNVPGISHNLDVRVPAAASISASDHSATIAPRAMAGPGPPSASRPQKPPGWGEEGGPPASGPPTRTRSVRSPPLPSRLPDGTSQYTQQQQEPQHQLAFGQQNGGQQQLPNSSFGGSNGQQSHVHFGHHRARSANRQQQQEQHPQVLSQIPAKSTSFLGRVSEGGNRSESCYETGAAAACDAAAADCDNNVHTWRDDPSLAQCDSVSCSPLPRFGGHPLFPKAVPVPAAVLSIAAVAAASAAVAGPAGLGLEPTGGSPPLQGTTLTVDYRSEAGFPSGGKVRNNSQVLLPPLLMRAPGQEGGERQTIFFGPGALPGLLVGSSAHETGDGLDGMRTGNTRALAGPMFLHVAPSIRSRQQVSGMVPSVVPCRSNDGDESASLWDAPSSPSSPAPDIDSAGNRLRCPMPSSAALLMSYRTYGMGGCTPVAVDTNPATAAVSGGTGEGAPAASAAVHGNATAQQQQQQEDCVPVPSNVSLAQLQPFHTAAQVPLELETATGRLEADAAPRASGGLIVVGTSGGGGSSCGGGGGGGTRLNDAGGEGDTQMATFAGGWLSSADLGGTSRRYCRGVSMHDNKAAGAGGCFMGSKERGGSSCGQGPAAAQGGSGGGADSGGSAYMSAGTSIAAIAAAYGSVPAPPPLKAALVRPLSANDVCGKGGDGGGGTVTSTVAYGDPEAEMPLARWQRAARAAGLTASIARSFRTLRNQHDENLVREDQLEVVGEIGAGSFARVSKCLYKPPDGGKPFLVAVKRIKTEMLSSTRDSEDLHLFIQEAQLLRKLNHPHIVKYIGLGVVLEEAPTRGGGGGGGGGEGDKLLGAEQLLALSRRSWGAPSARQQRNSLGAASRKLSKRESAKRLAAGGGVFGGDGRSVDKSLYGSDDTGPHPLLPAPVLRGVFIVEEFLGGGTLKGAVYQQMLRPQKQLYSNATALRWMAQVASALSYMHTSRPKVLHRDIKLDNVLLHRSKGKDSSDMSYEGSIAKLGDLGLAALLAHHRRASGIGASDLEMEECPPGRAANGRRLLTQSSCAVRQRVTAATMAPPHLRHAKDATQLSKMLESHKRERLGTYMYMAPEVYQRQKYDEKCDCFSFAMLLYEVFHRYITACSLEHAAQMEKYARQVSLGFRPAIHEDLPAPLRRVIKQCWAQEPEQRPDMAAVARELWTMEERGVLAALDLKQQQASDLLGDCCCVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJL6</accession>
    <name>A0ABQ5SJL6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014704</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042238">
        <property type="entry name" value="Rad28/ERCC8/Ckn1/ATCSA-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202:SF1">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="71"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="158"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="262"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="350"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="216"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="397" mass="43488" checksum="479F0EEB2ADC1F3D" modified="2025-10-08" version="1" fragment="single">MAQLRSNDFRRFICTSAGGVTCLDLDKTEDRFLLSGAADTTIALFDTHTGSEKAETLIKPLFSYRGRNTNEQGHKFMISSMAWYPVDTGLFVTGSYDCQVKVWDTNEVEVVSTFQLPMRVTGVAMSLVATSHTLIASSCEDTNVRLCDLTSGAITHVFSGHRIAVWCLAWSTHSEYEVISGDGGGQVRLWDIRRAGCRAVFDQHCTQWLPQARQGEAAQQNVSPSKRPRHASSTVADSMNTSSSAPALLRLESSSRMKETAVRAHNGAVTCVLPCPDAASLITAGTDGRMRLWDAEYRYNKLIGYEGTYNRALRGRQLAVTDDGRIVFYPSGTSVNVYEVETGHMLASVQGGHTESINCCAYNPSLHELYTGANDCKVCAWSICGDPDSNEDEDSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKG0</accession>
    <name>A0ABQ5SKG0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EIPR1-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_015369</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat EIPR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059104">
        <property type="entry name" value="Beta-prop_EIPR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040323">
        <property type="entry name" value="EIPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14205:SF15">
        <property type="entry name" value="EARP AND GARP COMPLEX-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14205">
        <property type="entry name" value="WD-REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23609">
        <property type="entry name" value="Beta-prop_EIPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EIPR1-like beta-propeller" evidence="5">
        <location>
            <begin position="6"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="204"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="395" mass="42308" checksum="0B76E28B71A11AF6" modified="2025-10-08" version="1">MGEPTTHYSLKYQARVLVAVPSDTITSKWLVGTTALREENEVRMLQFDADSEQLVCKLVYSHPAEVWDIAPCPTRDDVFVSVWAKGGNSSATLWKSVGDGGQASAQMQLEQQVELAGQSRAMRCALWSAQQTDTIVTLEESTLKKWSIAEAGAEATSSCPAGEMVQLWSGALHPRNPSLLCTAGSNDVQTWDLRNLGRPMGEIKMAHKMPVRSISFAPHNDTRVLTAGDDCKLRFWDLRNPGQALLELGGHRHWVWRALYNPVHDSLVASCSSDCCVNLYYTPNLATAPVTASATEVAGAPAAGGGTPAGPPATTPAATALGGTVVGAAGAAAVHKVPGRSGDLDGKVVSWDEHEDSVYGMGWSAAEPWLLASLSFDGRVTVNRVPKNIKYKILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKI2</accession>
    <name>A0ABQ5SKI2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1047"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1077"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1161"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1222"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1264" mass="130360" checksum="3DAE834F11056ED3" modified="2025-10-08" version="1">MPYPPANAEVTLEIRTTTVRHGRKLLRYSASSPLDTIYIKIPNTAPSSLHKPILEKLCNTVDLGHQRIVVGGGVRPGCALLALDLLRGSQELQDAFGELERRLRSEPELVVTEAARGACAISGEAVSSGSGGGGGGGRNRPSSFLEALNALTSAEGGAANEGIDPGTQCTAVLSSGLSRCLESMGVELLPGMLLQVQHLGAVVEAKFCGQEDGWAFRLLQPAAQALSIGTATSTAGASVPAGVSLFVNQQVFDAPTSAKEAVKVFVRIRGVMDLGADVGYGALGGSGSSSSGRYDFTVRGLGRHFPVLGVSVPTTPALDLTYTMGSTGEMVTSVDANNSCGSRGRGGPALVAAVASAVMAVAAADGAGEAAGHGDGGDADAAPAVVASVTVVNSQSNSASACSVSRHQHTPHRVPFSPAPSGGPGSDSGSDDSDSPPPVHWSQELPPPPPVQVPPPVHWSAADTVADAPAAVAAAVFPMADAAVCYYELTVAGLGAGHSGVGSAYVSAASGPLLLQLELWQDNCRLVASKPLLVLPPSLSGITSELQRLVQLQQGLQVEVDEADAKPAAATKAIGTVASAGDAGGSSADQLVTDMGMWLEFVHDELLVAQMEGVEVVGSTVAAVTEPQPPDGAAGSGGDAADQRYRRSPLLHPESSADAGSNVILPGSLDLRYEVLEQRRRSPVFSAAMLRTGLNLLEAMALHGSGTLVEHLLADFRSLGYGAAEVWAAAAAASSADGGGDDTGLSLLHLAAISGDVMTLRAVAELAVEAAAISANDGNGDSSPWLLRDGDGRTPLHLLAAAAGCGGAVTTAVAALEWALGSGSHVVRSAWLDSRDSYGFTPAQVLTAAAAEAAKIYRAADVVDHYPLGEASSPWQELNQARLPKQMASAPTSSDLTMAGAVGLIPPFAGAAGEVLMGIKNSVNQSAISRMEDSSRGPVAAADTKPRGALCEETVCKSVAAMARENVQEDVEKETKALSGPATAAASEAWNYGHQIWAEAVALLWASLRGFDACGGTKYSLHGGQMECSMVEVDEVQYQAYVSRRTLLLAQGWVIVHLLLLASAVRRLLVDGRPHELAAPLLYSSGHMMMLVVLRAVPGFRLYLPYRGFWWVLTAVLRSTSKVVQLLGWIPLPDATLKYVKGGATLVSDCVLPALFEPCPLPWAALILVCDFFVTAAVYLRIGYAAGLGHALLKAALKCSVSFSVRLAMDLMHRSAFLSGTPGQLGGRKSATGKGKRPQALPQTTTGSLVPPAAWHIRCKSKPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKK2</accession>
    <name>A0ABQ5SKK2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="139" mass="15232" checksum="769E5D08CF364F01" modified="2025-10-08" version="1" fragment="single">SPPPPSPPPPSPPPPEPPPPSPSPPPFLPLPPPRQPLRRPPHAPPPPDMPLADPPYTASSPPEVEFLPSPLLNRPPSPKPPRQARSPRPPPPDYEPPPPYTLLPPTDYFWTSPPSEARRLLAESEQLEVPESQERDNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL19</accession>
    <name>A0ABQ5SL19_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4263 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="37920" checksum="2727BA89ECD4B3C2" modified="2025-10-08" version="1">MEFARRHKAKGDIYNLTPEDLLREYESYAADGYAVLWDLSDHNITPRVLANLFTKIAESRVELKATMAIDLDANRLESAKPEDLNVVLQVLAKKPYLRVRFGFEVFVPYLKQAMEEANMLDVFGEQVVVNSPWTDQSLRAIASEQRRANNLMEKRLEKQEAKKDNPEQLKQMQGYRDTDDRLIEEGVARAVAELLEDGQVIFGTRYLYQSAVGDLDGLVVGRHDGRDVVVLVEAKHNMDTQARKAKSELFASVQTWEWLKGLSAEERESDETVLADYQALHLAEYSERAVVLAFGGSKFSESVVKKQFNLQMPWIYVVANSVGKFKAQTPPQVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL27</accession>
    <name>A0ABQ5SL27_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="86"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="575" mass="62544" checksum="28346DD337116ED1" modified="2025-10-08" version="1">MFELKNIFSCLSGTSKRHERSSMPSSEPTYQLCRSSRFGAETNDAVNKPSQIASQASSSESVGGPGNSQTWTLHRLLKQPPVQCHLKLVSTMEELLSGISLEQNPIICGHDSIVWQGVWHGAACNVKFLRVSSLTPELLAGIDLLITQSHPFILQYYLARAVHVPDGSRWRVVDGPALRPNSDEVNADDRSIGIESILGSTFFGANADQYSSFYQPETVEAVLEILQPKPEDFIIALVNEYCILGNVQRAISRRYFEHSVQFGAHAALRTAREIALGLMHLHLRGAAHGNLRPSQILLVESHADHRGFIAKIADAGLGSRHSLCQTPRATAHTAASGDEAASGSLQLNRPLSYYKAPELLQAGYGVRGEGVLPLTIAELQAADVYSFGCILYEILNGVPISHGRLSRDQKLPMATGKALKETLRTVDFPRLDVESCAWASGCDPQLQLLCCCCLSTHPQHRPGLGAVLAELNAIDVKMRTAARAARSVSKGCCGGADEDPQRQLSFSSLHVHGAPASVSLAPVSSLHRNSSNSDSCNFNREPIASQSRPKTELVTDWGEQRHLDPEQPLRPLCGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLD9</accession>
    <name>A0ABQ5SLD9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015822</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CFAP144 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029214">
        <property type="entry name" value="CFAP144"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33865">
        <property type="entry name" value="PROTEIN FAM183B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33865:SF3">
        <property type="entry name" value="PROTEIN FAM183B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14886">
        <property type="entry name" value="FAM183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16146" checksum="E632E24CA66817EE" modified="2025-10-08" version="1">MTMVPGKMDAVSVNRVWEEHVKKENRALQLNDQFAIPNPRKMDILPEKPNRTVPTPNPDEATVAASTATLHSLAAAKDVDKVPVDRFALPITGNMDYGFFHRVKPGEPNGMFNHKHKPCELTDYAQEYIKSFNGVGPYTTKLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLF7</accession>
    <name>A0ABQ5SLF7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pseudouridine synthase RsuA/RluA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015834</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF52">
        <property type="entry name" value="PSEUDOURIDINE SYNTHASE RSUA_RLUA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="2">
        <location>
            <begin position="227"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="420"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="498" mass="54847" checksum="012ED4A057197D70" modified="2025-10-08" version="1" fragment="single">QTSLPLLHSPDESGLMVLGRDDGTQVSSPSSRKDPSLLPFPRPPLPPSLSRAKRYLFQLSTLESALRIPPLDHQLGNHVVHLLVQRNGAASEVLSELLEVPQELMVRLIWFGAVYYCPVPPLPRSATPTGSRDPQDAAVIPRCQSASTAGGDVHRDGQLVELQAIRQEALAKWGRHSRHQTPRRLDSDQALSRGGYLRVHMHPKRFPVAHALSHEQWSRRLLAVRPDHVVVNKPPGLQVPPTVDNVQESLLACVEKALSLKPGSLHPAHRLDAGTEGVVVLARDAAFASYFRGLMANKFQHAVRKQYKCLVSQAPPLGPLVHWILEDQRRQGEMAHSVVVPEGTAGAARCELWVEQVEQTELNAEAVDLWGAKEACEVTITLITGRTHQVRVQMAAIGLPLLGDRLYTALASKCQHQRWGQSEGGVEQRQQQEQGWGEDEKQQQGAPGRVQDWCLPALMQPLAPIALQAHSLTVLEDGKMGPGPMSFAAGNPWWRTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLJ3</accession>
    <name>A0ABQ5SLJ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hydroxylamine reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_015455</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00069">
        <property type="entry name" value="Hydroxylam_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010048">
        <property type="entry name" value="Hydroxylam_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016100">
        <property type="entry name" value="Prismane_a-bundle"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01703">
        <property type="entry name" value="hybrid_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003658">
        <property type="entry name" value="PRK05290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="HYDROXYLAMINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109:SF0">
        <property type="entry name" value="HYDROXYLAMINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="Prismane protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="654" mass="70775" checksum="4842E5A48A6CC4B9" modified="2025-10-08" version="1">MLSRCLSSVGSRLGGQMSRAKLCLFRVGAAVHPLGRSTQKIFAYLPPAAEGQSCQQVRDMASIKARMEAANQALKDDKMMCYQCEQTKGGVGCTEIGVCGKTPEVAALQDLLIYTVKGLGSLAHHARTLAGLEDQEVNTFINGAIFSTLTNVNFADDRFVEFVNDSRRLHKQLAAKLTSAGIYLPASSTTPQPWFGSMPHPMAWNTQSMAPRGVADMIEVGHQTGITVRQEALGETLAGLHELLMYGLKGLAAYAHHAEALGYKDPAVYADVQESLAFLCSPEAADVGAVLDHCFKAGATNFRVMEMLSNAHTSTFGHPVPTPVTLNPVPGKAILVTGHDMHDLHMLLEQTAGKGINVYTHGEMLPAHGYPGLKKYPHLVGHFGGAWYRQKIDFACFPGAIAVTTNCVLDPLQAYKDNIFTINETGLSGVPHIRADGSGHKDFSAIIKRALELPGFTAEDVANASKKKDVMVGFGHNAVLSVAPQVVEAIQTGRLEHIFLVGGCDGSEPQRKYYSKLYQYMPSNTMVLTLGCGKFRIFDQDFGMLPGTNIPRLLDMGQCNDAYSALVVATELSKVFKTDVNSLPLSLDLSWFEQKAVAVLLTLLHLGVRNIRLGPRLPAFLTPEAVSVLVDKFNLIPANVADPAADMKMMMQCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLS4</accession>
    <name>A0ABQ5SLS4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Leucine carboxyl methyltransferase 1 homolog</fullName>
            <ecNumber evidence="6">2.1.1.233</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_016052</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in brassinosteroid (BR) signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[phosphatase 2A protein]-C-terminal L-leucine + S-adenosyl-L-methionine = [phosphatase 2A protein]-C-terminal L-leucine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:48544"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="2.1.1.233"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.233" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016651">
        <property type="entry name" value="LCMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600:SF21">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016305">
        <property type="entry name" value="LCM_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="392" mass="42651" checksum="06F4163C66B7C1FA" modified="2025-10-08" version="1">MLRSLEGSDASVQGTNDDAQISKMSCAKTGYFKDEFIQYFVRRASRRSPLINRGYYSRHAALRQLLERFLGAAAAAGEQPQVLVLGAGFDTTWFQLCRDKGRAPYRCLEVDFREVTRRKAAIIAKESALKSLLVQSPSLAAEAADVDYAPEAPESTCSVGPSADSSDSNSALTITAGRASGAATAVATPTAAVESSAGMDVAAAANDVAVDVTGGTVCSPYYCLQAVDLRNLDALELAVQEAGWDVRLPTYILSECVLVYMEPSDSAAVVRWTAQTFPNAVMAIYEQIRPDDAFGRQMVSNLEMRGCPLKGLPATPTLTAHCARLTDGGWNWAEARDMDHLYRTFLNAKDKASVERLELFDELEEWHMIQEHYCIALGRNDAAGWLCKVGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM29</accession>
    <name>A0ABQ5SM29_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRO1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015454</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BROX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038898">
        <property type="entry name" value="BROX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23032">
        <property type="entry name" value="BRO1 DOMAIN-CONTAINING PROTEIN BROX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23032:SF13">
        <property type="entry name" value="BRO1 DOMAIN-CONTAINING PROTEIN BROX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="2">
        <location>
            <begin position="77"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="466" mass="48445" checksum="7531DDE23E29D27F" modified="2025-10-08" version="1">MSTMHRNCSLTLNWYEPLRLLRTKPYDYSLLSKQGLGPLSTVTLACPNFLNELTFRRGAIGMWLQSLNNGCSPDGAPAPDVALEGLSSLAARLEGLFRAIAAAAGASGTDSTVKPAAAAAGCITATAALTMGFMHSEWNSALSRKPQRYLRLDCLPQELAMVYSAYGAALRHSALQHYSQGLLLGAVDTAGGAVSSGGDESGGGDQQSAAAAAGLQAALTRAVAGLRRAAGVYGYLADEVLPALREVMAAAAAAAASRDWPLELLPAAALCMKHLSLAEAQALVAAMAELKGMSPGTQRALHAGCVLLFRDAAVAAQELAAAAPLGLPLSDRLLVRFLPAATALHAARSQLCFAREQQAVMEMGQAEAACEEGLRLLVSAAGCLDKRLDPALWLELIQAEQATCSALHSAVHRDRLAITFQPLPKQPPDALANPAIRVVPDVFQPEVVFPPAPPASAGNKGGCAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNY5</accession>
    <name>A0ABQ5SNY5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000105">
        <property type="protein sequence ID" value="GLI71336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="179" mass="18519" checksum="DAF8B5EE3E62E460" modified="2025-10-08" version="1">MTYAMLTGLPQKYEVIVSILTTQSGELTLSSATAQLLAFEARVGSMHEASTSSSAFVSAGRGSSATGVRPDVEEATSAADHGSHCSAGTVESPGIWRGGALPPGWLRGLAHGARRLEDPVMGQAAWSAQEWAAMERPRAGPAWGATAEVMAGTTMWTVPATGAMLARGTLPLWHLGLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP64</accession>
    <name>A0ABQ5SP64_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_017186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000125">
        <property type="protein sequence ID" value="GLI71805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="166" mass="18255" checksum="5431CD87CDC61B7A" modified="2025-10-08" version="1">MLIHAESRTRRPWRRPRLCSPPCHVKSEMPCHATALAVEEVECGTCNAALDSDQGGLEASTVTVCAQLGEAAEPKAAFPATMCEQQDETGILAAPLAQQQAEMEVSPVTLDSQPVEMEALVTYLSPQDEAEAWEAWAAAEYARMQEELEHYDSPNKPIKMLKEVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP86</accession>
    <name>A0ABQ5SP86_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092:SF2">
        <property type="entry name" value="HERCYNYLCYSTEINE SULFOXIDE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092">
        <property type="entry name" value="L-CYSTEINE DESULFHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="61584" checksum="D81B5DD92952F98C" modified="2025-10-08" version="1">MAPPSSTPVVTRTGYQNFGSFFTENVEDLICIPKDSYIAPEPPFTPPSLGDIATLDFGEPCRQLFNIDFENWTFINHGAFGAVCRAAHQESNQWREHCESQPLRFLDRQLFPHMVRVMREMADFIGADPRDLVFVPNATTGLNVAIQAAGLRPGDTMYMLNIGYGSVKKMAQAALAAAAGRGAGTAPAPAPAPGADITGGEAPGDINVVYGEVTFPLSSPRDIVDLVAATMPPNTRLAVFDSVTSNTALVMPVSELTRLCKSRGVQVLIDGAHALGQLHLDMRRGICGGTYHDSGSSSSNSGTKGDGHQTRPLQQVGSPATASVSSTAATVEAVNIAAATATAADVVADVVADVAADVAASAGPQGSAPGNLVSPELDCVPDYFVSNCHKWLCAPRGSAILWVAPNRQAFVRPLVVSHGSGCGFTSDFIWDGCRDYAPYLATSSALALWRLLGPPRIRAYCRGLLGEAVALLTARWGTTMLAPFLQMCGCMALVELPRAGLAETEPATSTDAKYVQDLLHHVHAVECPVKCIQGRLYVRISVHIYNILSDYERLAEAVDRIAASNSPSQAQAPDPDPAPNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPQ7</accession>
    <name>A0ABQ5SPQ7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="349" mass="37104" checksum="E551A3E3AE18351F" modified="2025-10-08" version="1">MPATVSIPVATPSINVHLTVPTMDGQLCNTTETPYNIKLAEYILRLSPSPPSPGTGPIATILIGVDYLGTLPVLDGAEDNPERIFQNLKCSPITIVGQTSEDGKCPPQRQVQLKLMPIYSPSPIRSPSDKSDTYAPRSSSCGSCISSQSSSSTGTGPVANSSNSMFEAALSSPLPLPISKISCTQEDGPNQSNRDNGGPEVEPPHIGGISESDGLRTGYHMRPLGRRPSLPLLSNIDGHLPLEYEALMEADMDDLLARDLIHWTPEQLVEDALERALKSVKTASGQVRARSKAAVVPQTPGKQPLAAARAVEAGKASGGRKPRRHKRSLMKRLVWSLFGACSGCSVNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPW6</accession>
    <name>A0ABQ5SPW6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_017229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000128">
        <property type="protein sequence ID" value="GLI71834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="26820" checksum="D8DEEBC180E54246" modified="2025-10-08" version="1" fragment="single">MQASNLANAVSALAANTFQFVGRIAANASQANRWEWLSRLTQPPNAAVAGGIDLPNQPSPIPRQGGTASPEAPTSPIYAPSPFYVHGPAVVSNHLTPGAVDIPGMAPAAAATTCAAVAGTSSRPPGPPHTGHEDEVIKIAIAVPKLNIPMDVKLSDLGRVAAPLTRAFVRNVRNYFATARGGQQDSTRRLFLATAFEGAARLWHDQWTNARDSYTSEELLEALLVRFAPQVQSRDTEARLKLNAGKYRMRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ42</accession>
    <name>A0ABQ5SQ42_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016713</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044208">
        <property type="entry name" value="FKBP19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47717">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP19, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47717:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP19, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="2">
        <location>
            <begin position="123"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="263" mass="28898" checksum="97A5A7213D155C2A" modified="2025-10-08" version="1">MMRSLHGSGRVASTTIERSIINARPPKGRVHVVKRMVKVVADQVDAGFWRAESDSNGADVERGDSRPVFGRRSAMLAISSMLNCLSTAAVLPELAHADDFVTLPSGIKVLNIREGEGAQPQPGDTVVVHWAGFTKGYQGKRIDNTSIRDEPYEFKLGGGQAIKAFELAVENMRAGGIVRVEVPGDHPELGYPLDRGERFTGELVSTDLKIYKYRYGPQPAELGGQRALDFVLDNRTLKDFNRTLLFDIKLLAVRRGTSVPVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM97</accession>
    <name>A0ABQ5RM97_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000458</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050145">
        <property type="entry name" value="Centrin_CML-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23050">
        <property type="entry name" value="CALCIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="186" mass="21375" checksum="6C2127F521A8A63E" modified="2025-10-08" version="1">MTAAVPIAKKKRLGEGSKYSRSDVLKLKDLFDSHDLDNDGRVTANELITALQGCYLRQGALSTFRSVDMNKNCVLTFNEYLRSIYPYANDREHKAMLAWVKRTPREEQPEPMFEPTPQQLREIAKMFERFDNNRDGVIEVQELVATSAALGYDESEVEELFRNTDKDMDGTISFAEFIDLMKVSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMN2</accession>
    <name>A0ABQ5RMN2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_000591</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="267"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI58827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="403" mass="45276" checksum="74068D7FA9F2875C" modified="2025-10-08" version="1" fragment="single">MVPFYGVTSWLSLMYRENSIYFDVPRDCYEAWVIYNFLSLCMAYVGGPGAVVVKSEGKLIKPSWMLMTCCWPPIKVDGFFLRKCKQGTLQFVIAKPILASMTLILFAGGMYEDGDWSWKKGFLYISIIYNTCYTIALYYLLLFYVGCEELLEPYRPLLKLILIKAVIFLTFWQSIAISMFSSKFKDPLDAAALQDWMVCMEMLMSACMMWAAFPHTEYKMGGQTTGWRLTAFLHAISLQDVYSDIMHQFNPNYKTYVLYSDGGPSENVKRKKFRAGGKKQTRKRLSTSGELVAMEEGGRSKGPRQAQRTNSSGARAPVFSAADGEGEEWNPPPKPTNKAAAFFGKPGTGKGVFGWLPAISEAERKQAVLVDSESDAEYDDDNLQETALDHEMLATSAGSDEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMN3</accession>
    <name>A0ABQ5RMN3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006734">
        <property type="entry name" value="PLATZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31065:SF1">
        <property type="entry name" value="OS09G0116050 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31065">
        <property type="entry name" value="PLATZ TRANSCRIPTION FACTOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04640">
        <property type="entry name" value="PLATZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="130" mass="14908" checksum="72A74F3B015D7B7B" modified="2025-10-08" version="1" fragment="single">LMDAAEGLFTPCPQCHELHNGSRDSHVSFLDVDDPFGRAYCHFCRPVSAGARVVQVRRNTYHDVMRIGDMSMLYDISGIQQYTINNGKVIFLRPRPQMPKQGYVPNCYYCHRSLMDIGSRFCSLECKLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMZ8</accession>
    <name>A0ABQ5RMZ8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000002">
        <property type="protein sequence ID" value="GLI58387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="234" mass="27533" checksum="3E56110CDB99E151" modified="2025-10-08" version="1">MEPFFSLDGMELQQITNVQQQQQQQDIQLKQLEQQLNERDQQLQQQVDVKLQNVAQQQDGKLLEIMRQFTRQQDEKMRQFTQQQDEKMQDFTRQQDEKMRKFMVQQDEKMQDFARQLLDEKMRQFMQQAEVIRENQVARSYNVFAIDADHTLEPLLVESVQAQHALGTAPSAVPVNFPATVDAMSKLTNHDLDQLQDFYNTKFSGKSIKTRRCRFAVYIGATGVIRQAGFYMAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNS4</accession>
    <name>A0ABQ5RNS4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000928</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 4 ARMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="652"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1084" mass="112374" checksum="B8CB78B0DCAAE9D0" modified="2025-10-08" version="1">MSAYLGISNAYFHNLQQQISDVSNRTSHLEHMMNLVMQHIQLKSDNFPEDFSSRNPLAVLPLPPDKHRPNHNHYHTAHSEAVPTRPTSTPLGAAKAAKGCFWLVGDGGIGRQDGAAVAAERPAAQPPLLPQQLQPRFNTAAADAAMAAARKAIADVASAAASETDHSGRISAPGSAPVSPSPPYGFRRLDHLGDSERSPDQGPGDASGGSATGGGGSGSGGDSGPVGSGGGGQLRSRGLTLNSGALMRSADLRPDQGARLLRQQPQQPQQQQEEGKGGIRAEAGGWQWGSPCKSVWSTGDDLSAYSAASTAMSQPTTFKLPPRRQVGTVPWVIANQILQLPEESPMGLLQDGGYDDVIMTEAVVEMGPSAAAARVEPFRNRGSGGEVNPSGQEAFPDIGSEDVLQRILDSVGFDAGPWVNILVAADAAAYDNTNRHPCGGAVVIAATDDNDKAEAAPLPLPPLPAIFARLGLRRGAQAALVMMVQCREHPAARVMVSESPDCLRAIAKMLQTGSPLEQRIAAHVLWFAGRSYVGGKALYKTCTEPPPSPQLQPVDADGDVAPSPPGLIGVLMAATASHDIHTAKAAALALHNAIVAEGGAQAVEDAGGCGVVSALLDMLASSDDIGGEAAAATLAAAAERSPFMRGEIMEGGGVEALYKLLSYGAPSTQLSAAGALEHLTMEVEGAARLAGCGGVRQVVELVRHGVGEAPVRAIGILRNLADVAECHHCMMRAGVSSLLMGTLAAPLPHQFRKGTAAASAAELGVGGSDGGWDEEEEEEVEEEEEVEVQGAYEIRVLAVSTLVNLATRGQADVRQALVAAAPAGGVGGLRSLMSVVANAPGWDAETVAWALEGVLALTADGPFRGEIVRQGAVQVLQQVLQEGSPRQQELAAQALANLTTGAGDQDKVAMLEDGAAEVLVSALRNGTPATRHAACCVVQNLARCPACRGPLLAADAAEALTATAIECDQRVAAAAVRALYNISCSIRGCKDLVTRGSVGDLLAALRRFTPDLLPLEPSPFGYGTVAGAVNCWDSRTHCGPADFRANWRVVELLVKVLKRLGLYGDEKGIVRQEVEAALANASIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP19</accession>
    <name>A0ABQ5RP19_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028131">
        <property type="entry name" value="VASH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15750:SF2">
        <property type="entry name" value="VASOHIBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15750">
        <property type="entry name" value="VASOHIBIN-1-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14822">
        <property type="entry name" value="Vasohibin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="337"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="577" mass="61214" checksum="38C24ED397952D17" modified="2025-10-08" version="1">MPLSSAALQLQRLLNSLPLPDERISEAEKLIKECSEADLAQLGLGPPPRPVVPRSLIDGGKGGGDGKPSGGPQQRLHTVQQVINSLQYNHTPGYYYNVSKSRPFSRIMDTAREALRVALPIKCLEAVFLGALMTAGWQDLDRLPLAFKSTVQGQTYRHIVLAVFHAPSRTWGALGLSRRPELMDKNLEYESLADLVSEYKSSYERWWHTLARVYVGLPLEHDTYYAGPVCWRYLTLSLTGRKSWMAHRVALDKFAGQARRLAAKFRALGGKPTSETSLGAADLNPAVSIGMVLTTTAVASSLYAGSAVGRCAKSASPARRRPTLPPPPPPPPPPPLTTTLTRRPGTPSRLQASPRPPPKTATRLPPQGSSVTVAAALTATATTTAAPTNAGAQGVSGSGKSPSGAAAIHSTAFPASVFYGTGGHTEINRLSAAANDIDSGNVAASATLTPPSFVLEVVNGDGSGADAVERIAELALDSQPSSIRARGFRTQPHRSSDPGSGAVLAVAAVAAPQERRRSGRVQARILRQQLKAEEQSGEVNAGREGSTAVLRCEEGEEEDEDEDSDSDEGDYMSDEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRC1</accession>
    <name>A0ABQ5RRC1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001579</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="52"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="956"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="932"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="932"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1099" mass="110404" checksum="80AA7032001AF6C8" modified="2025-10-08" version="1">MALRGLGRLVTYSFSGAALGCLILAAIVSAGRLDSSHASSRNLLAGTRADEFPYCRCELYSCRCSPYTTKYLGAAPVAGGGARHCFKVGYGGCPPDTPCCTSLELNVHKISFSIGGSCQKPVISQVEINNSKWLSWGTSAWPLAADAGTGYDIRIYNLRFNASTFDGTTICLKASGQCLSIQQLCDTPTGDCEFSVFDSHDTSRYCPVCPVMLLNPPPSPFPPSPPSPSPPSPSPPPSPSPPSPSPPPSPSPPSPSPPSPSPPSPPPSPSPPSPSPPPSPSPPSPSPPPSPSPPSPSPPPSPSPPAPSPPPSPSPPSPSPPPSPSPPSPSPPSPSPPSPSPPPSPSPPSPSPPSPSPPSPSPPPSPSPPSPSPPSPSPPSPSPPPSPSPPSPSPPSPSPPSPSQPPSPSPPSPSPPPSPSPPSPSPPPSPSPPSPSPPPSPSPPSPSPPSPSPPSPSPPPSPSPPSPSPPSPSPPSPSPPPSPSPPSPSPPPSPSPPSPSPPPSPSPPSPSPPPSPSPPSPSPPPSPSPPSPSPPPSPSPPAPSPPPSPSPPSPSQPPSPSPPSPSPPSPSPPSPSPPPSPSPPSPSPPSPSPPSPSPPPSPSPPSPSPPSPLPPSPSPSSPSPPSPSPPFPSPPSPSPPPSPSPPSPSPPSPSPPSPPPSPSPPSPSPPSPSPPPSPLPPSPSPPSPSPSPSPPSPSPPSPSPPSPSPPPSPLPPSPSPPPSPFPPAPSSPPSPSPPSPSPPPSPSPPSPFTPPSPSPPLPEPPELSPVCVFCASVTIEPPQTLDNTGKLYTFGNPVNCTRVFDLIVSDLTGAFSAADVSIVDPLVKASCSGAFNVSMSPPVLPRLFVCGASGIPGNLSALESAFYDLIERWVGSVVGVPMMIPEMNNAIVCPIGLSGYRFSGAVESVSKDPQCPNAFRAVDCADRYVPIPPSPSVPSAPRRPPPPTPPFLKTHDCSQAASLAPYALDKIELYNYTDNRNNRLVALCTGIEEKSCRPSSPCCTMDVAKVEFLIKTSCQGAVRRVTVGDKEVSYSRNFYPGFTSLKVVKLSTLDEPVGTRLCVYTLPGSCSTPEGLCSGDRCQASIFSSNNKCCPTFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS75</accession>
    <name>A0ABQ5RS75_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Steroid 5-alpha reductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_002590</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF11">
        <property type="entry name" value="PROTEIN C-TERMINAL S-ISOPRENYLCYSTEINE CARBOXYL O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="52"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI60437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="44666" checksum="30E49E64B01BEC55" modified="2025-10-08" version="1">MKTSYLCQGSTSAHLRVRRALAAGCLHLRHIKRGVAFTRPNISLIAKVSHGDIHHSDTKNLSRPTDPVDAPCTRKPAGQPTNPGLTQGNNFPSSSLASPPPPAIPPAPSVPTSQKALDDDHFADVWKPPPGEQPLWWVEKFEARRKAEARRKAEDPAFVTTLERVALGGPGRASTNNPALRFLKAALQVLQLVVVLALLLLLPAVLHGGGLSVAKPFTTFGLYFVFFGLGTLGRVVQYGKLAPRKQDRQISTWSSRLSFVAFVVVVPILHFVAMYRFVGLALYTSAMSYTLTLYDIIGGVGMILATILNALAARELGASYDRVTVPAALVTSGPYKYIQHPIYTSYMLLFFSYGMWLHSAPTALLMLVVCGLYYRGRTRLEARVLEGAFGGYYRDYASRTKSFIPLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS87</accession>
    <name>A0ABQ5RS87_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="776" mass="78042" checksum="E8550E2D54AE7D7B" modified="2025-10-08" version="1">MLNTRTDASMGRICGPNLAAAHPHIAATAAVADAAVKRIATLPHGPLAAPNCGAAKGCGDSTTLNTAVLAAGAAGAAAHPTASTTSSTGSNSGCESEATAVSRERWQQHVAPRGRPEAATAAAVTPPCEQEMQMLSAVPNLSAVGACDIGGGRGTAGGGKFAPGFGPPLSVNPPPAATGWVPWGCVDCSGVQNPAAAAAATAAAASSVPSSRAQSLDGTHPTSAGGAAPPGPLPQPLHHLAVPLYPVTPYRYSPYARPWGAQSFGPVAAPVPVPVPVPVHTPAAGPMPTVPSAAGGCVRRLRLGTYYHPAALPPPCFSGLRPLPRAASPAAPPVPLVQWPAPPYAGGWPNNSALAPPGFVDDYPMVTGSTHAVHNALCGPHLPPQHLPPVVPPPYIHGMYGQLSSACGGGASSTHGLSRDGCRTDLICSGRSSAGGAEIAVGGDAAAADAMAATAARCILDGAGETDAAAASRETDAAGALQALAQASPAPPGRINPWVSRLPSSGGSAAAAAAVTGELPRNMTSQHPQFNSLRTAPVAIPAPAHAPGPGPGPGPVGAQYSALDAQLWQQAVAVAYPSTAWTSCRRTQYGLTNVDVAAAAAEAAAATAAAPIGLALMRVGSLPTPPSCTYRSPYDFRYGYECSYGAMYDCGSSAPDGVIPRATPITGAGVTAAARWSRSAVRDGDGDCDGDGGVPVKNSIAAATAATNNAANHDDGAPEDVACKSVHMPHQSMPLWHSGYGEHWPWPRYPKMPYMYEHRLPYSYSQVPPLVKPWSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSF6</accession>
    <name>A0ABQ5RSF6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_002653</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000126">
        <property type="entry name" value="V8_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF465">
        <property type="entry name" value="EGG PEPTIDE SPERACT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00673">
        <property type="entry name" value="V8_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046929171" evidence="9">
        <location>
            <begin position="22"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="11">
        <location>
            <begin position="20"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="570"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="706"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="834"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="964"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="1093"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="153"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="253"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1210"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="159"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1210"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1264"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1302"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1317"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI60492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1378" mass="146586" checksum="4F9D6F3A3BF18EE4" modified="2025-10-08" version="1" fragment="single">MAMRLFNAVALTALLNGLASGELYRCGFCSGGNYDPVCTTAGITLPSVCFAKCQGLRVVRKGHCQVSDTLSRSLHLPGVTAVASLDSAVESVSNTPSEGVRSASIDLFDAEEVTTRFAGEGLAYVGHARMLDSAPTGAGSSGFVGRLSMAASGGGGSSGSSSSTINSSTSSSSSSSSSSSSSSDSTMNGASNDAMLPNTLTVRAIRVTADGAVFLEIRPLNLDLNNLPTSGESESRVGDDAEVAAAAAAVAEAATAESQPSTEVDPSEPVSRRRRAQEDMDEYLGGGAKEGDDGGAAGGSVQGRQLGRRWRRRRQLIDDRYPAEVLAWPYRTIGQLMFRTSGNAHICSGTWISRYDVLTAGHCVFNVTGGTGYRDFTFTPGKAGYIAPQGIVKFYYVTFYRAEYPRIDKDGVNYFDIALIRMTTPTLSYMGIKFSCTKVDYPKAQTCGYPYDRNGFYVQKCDDCYYTSNACNPIVQPINWCYTVGGQSGSAIYDLSDYHILGVLSGGPNSGMYFQDYTVWTPIDAIHFASLSRWLWKPDSPYNAIPSAPPMTPAVQPSYIGEACDVVGLLRLVASSTYGAGRVEICNNGVWGTVCSTAAFNNLNARVVCRNLGFKNGSWVVPSFFNPPDPRQSAWLELNCAGNETGIFDCPPLRNANWTAGPICSQHDMYDLSVICRDTASVVGSSSITDFNRTVGYYPCTEDGAVRLRDNLNSSSGRVEYCHSRRWGTVCHDGWDDLDATVVCRQLGFQYGIALKGVVANGQSPPGPALMKIWLQQVNCNGSEAELQDCPIQVPVGSTDCSHRADAGVVCSNTPFAAPGVPYYPSQCTEPGSIRLELNASRVPSAPGTIAGRLEVCYAGQWGAVCEPNFNDPDARIACRQLGYTYGRAIPVNPNPGQPIWMSEVFCESTFSYSYSFWPSRITACRFWGMEDVNCTYGMVGGIQCSNVSTFLPPNLECSTPGEVQLRNGVSNNIGRVEVCTPNRVWGTVCEDGFGMAEADVVCRQLGFTSGRVLPSGATPDGHPDSLSALAGLACSGNERTLLECPLEQATMRCASHRLDVGVICTNSVPPPVPAPVSAPPPDPNYCPVAGQARLLDGFDRVAFDAGRIELCLNGLWGTVCGDGFQSINAVVACRHLGFTTGIPVRPDDPDGFGYGNVYQPVHLGILSCMGDEDRLTSCINTRSTDCPHTKDIGVRCFWFGIPPPLLPPRSVPYPKSPPPSPPPPKRPLPSPHHPSPRPPKPPSPRPPPPSPPFIPGFVAPTPLRQPPMKPVLPPPPPPPPPPPPPPPPLLPPPTLTPPQLPLSAPFLLPTLGLPSPSKPLSPPPSMLPAAPSPLLPPLLSPLPLTPPPTSPTPPPPPFPSPPPPPSPSPPPPPSPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSU1</accession>
    <name>A0ABQ5RSU1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_002879</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06133">
        <property type="entry name" value="ERI-1_3'hExo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051274">
        <property type="entry name" value="3-5_Exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047201">
        <property type="entry name" value="ERI-1_3'hExo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044">
        <property type="entry name" value="3'-5' EXONUCLEASE ERI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044:SF61">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="9">
        <location>
            <begin position="464"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="25"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="359"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="367"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="523" mass="56984" checksum="406897A7115F6819" modified="2025-10-08" version="1">MSQAAGAPIRNAFDVLMSRRHEISTSANKRSLGKSKPSKVQASLSTASSTTNTSRSSILTKRISLIPAEEAGSANPAAVMLQPLGPLDKKRVASEPPIRVGLRTSAEANARVKNQVSTDDLVEMPGTSGRTLPDEPQPKRPRLMATLRKSTTKTLSPQVHDLFLVLDLEATCTKRRDLFPIEIIEFSALLLDAHTLATLGEFQSYVRPTEYPRLDPFCVDLTGIQQEQVDTAPLLPVVLLRLKQWLEGLGALQEGRSLLPVTWTDWDLKVCLETECEWRKLERLPFLRRWCNLKRVYTNRYRRTNSLQKCVEAVGLRWHGRAHSGLDDSRNTAALMAKMVRDGCVLQVTDAFKDTAPVPGQAPSQIPAPALFPAAPPGAEGPERGHGGGTDPCDYFPISRSPCKEASGDADVSATQRGWADVVGSSPGSGTLRQTVLTLSSGPAAAAAAEVTLYDSAMRWLGKCKCGVPAHFRTTKKPGANLGRQFYSCGRWSISDRSKQCNFFAWADQLQMASGVAGRGPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU01</accession>
    <name>A0ABQ5RU01_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12370">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1244"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1446"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="449"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="685"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="754"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="779"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1250"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1467"/>
            <end position="1482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1833" mass="191414" checksum="DA30F52944B5EA3B" modified="2025-10-08" version="1">MGAGASAQSGIFGPPDGTLDPEAEWRSENVRELDRLGGNQRPAIKRGESTKYRLEWLKEALHEQSDRLEEALAKDPLAGRHEIIRQQAAKLARNLNVNHHRFTVREWQTMKHTDLRALQAREEIERRLQERDKSGAPDQDHSPERPAWNALHWSANEFRKSAVKLQLPSPGSPTGPSATSPSGSKLLGLASAPATEPADDLGAQALEHRPSSPSFCSMLPPGVAPVPAGLLGALVAETGEEADAALRVARPLATNRTSEPPAEWRLRIIRAKRKAMMVISVVLAFRGRKYRARGLQRLDSLSYFIAQHLGPDLQRFTEEQMMYRHTRATAADLQALAEEVWRQKNYRDHPPFQEMCMGGSLLVSDVAEAGDSMLMAAHGGSARYPLGPGSPGGNGGGFGAFKPSAAPQLRLSLPAAGFSCRANSPLLRSRPTNLTPTMHHHGPAVSGNGASPSLPASSQALGAAGGIGPPRNIHPPAGSHQPPLASSNSVGSAAAAAASSTSLSGSLPVLCGHSSGRISMPSGLAAPPGLEAENSGGTAAAVSVGVVVGSGVKNARFSAPGCELLPEDDPGGVGSGDALPVAVPAPVVPPQPSPLPSPSRSTTKRWSSPRFARRSTACSGTDGGEGSARVPHGPATSSGSIGTRRVLSAVAGEDMSMVARAQSWAGPSPLQPLVTNGGGSGGSNARSTSNDNMPFSPSGMSPGAVLPAPPALPSPVGRNPRNRREPGLVLTGSASTASLPQAGPQRPPLTRSPTTTGDGCTAAAASAAVATVPPRNSRRAGSVTRSGSAASSGGGAGIRRRPARVGGSATGEPGCSPLRSVSPYAERLGGVASRRSIRCNSTIRRMVRDGSSDQLFTRYQEEAAALERPLRMMHLFANRANARWVEELADAQPAVPGYGSGIGSTNALTALNASGVGGSSSVNGNGAAAPLGPGWETPYVYDGLRFSAAVGAAQASVFRAEVATMLRAGRMAVQLLAGDEYMVLGDGQTQPLWSADPWDLIASSFPAKAAVADRVRAVHLGPRLLMALVLAVEALAGPEAVAKLVVVLHINDSQQAEVVRELRFRKYCGLRPENIILVVEQRRPGYYWKPEQTVFVAENSAPPASVGSGYAIMQLMWPEEALQIPPEEAKKTAAAEAPSWAKASGATAVAAAAAAAAAAAEPPPLVSLGTSVLDHLTRHGVEWLLTRRLRDISLYNPDLTLDSEVLSYMLYLHDHLGANMAVQVAMMDSLTAARSAQTGIVLAPPRPRRATSTGSGAASVAAAATPSRLGTSMSPRRAVTYAGGSTSSGGGLPLRAGSDVASASQPEGGAPGGGLHPHFVVDIKNSDTQTPRAIQMLTDMRSRSKCKLAVSTQRYLWRLDVLQSLVQRASIFHPCLEVADELVYLTFDMADLTAATGFGARCAAVSGGPRPVRCLNTPTDLEELLGAILAQDTHAPFRRLAANVNPAYGPTRGATTDRSPPRVNLHPAVSGAGPAPAEAPNGDCSAQPHHPASASGGGAAAGYAIGPTSDLRSLNTGQRIVMLLADNESTSMAVQLLMALVKPGRDRVILVTMVPSIIQEANGRMLLRKHEMSMNKTMVDVKTELLTKGTSGSLIEQLESFIDEIDPQLVVMGSQVLASASTLGGSPSLGQSPLSSNTSGGGASVGGVASLGTATTAYSSGSSLSNAAQGAVLGSVAVSLLRNSTRPMLIVKANAKYASVLWDKDKLRVVLEVHHSSRNLLRYVCSKMISPPRHDKVFLMRGGTKDPSQHETMTSRRLLESFSDIATQHKVGVVRRALEESFEAGAIKWADHDKAHIIAVHAAAGRGLSNTTLHILRAARSAVLVYKSNEQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUQ1</accession>
    <name>A0ABQ5RUQ1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003453</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="546"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="621"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="458"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="581"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="705" mass="71426" checksum="38A3C9F82B603132" modified="2025-10-08" version="1">MDRKPWSAAAAVFRTAQLLWLLPGGIPLLLMTALPVTSQTRLVSDAASLYAAFGDETITHIQLANSITISNNKGTEVFPILLGRNCLVDGAPGSSYPRLDFNFATNDRVQLGAGVTLTFRGLVLANVISRSQISLDFFAQSASSSSIVWESTVDRRRICQPLQDIVNAWNKAPRPAGVGGPSQPQQQEILNGSNCFGEEGCYSTPVLRTINAVVDSPAGYRLRWNDTLSVCEQFISDACIQSLGSDLCYLNSIEKLLSAGSSPDTRPSAAPAKGGLSSGAQAGIIAGCLVGVLLLLLATLLICRWRRRHPDGDRLAAAAASAGGGRAGRGSGSGDAQCTFSGILYPNSEAGLQAFGSSRSVDVAAANAVQDHQQNQAKLPGGTAAVLLRSGGGGGGGGTGEEAEPRPSEDYIPGPALESAGSPYQAGSVLSAGLRPSGSGTRDLPYSSSLNHPGLPCHSQLSHTTSQSTQQPHHVGLHPATSPGSHQTHSLGSVVLSMLRTVLHRAGSLTQSHSGNRVPNMSVSGTLGSFGGSAAAAANPGPVAPGGTGASASGGVGVPRSGGTAVSSAAALRNGPSGRSGAPGSGRGSSGGQLALASYPNRPSISEIRQELERMEDHWRREGRTGGARLQPATSASARRSPKRTRQGGSGGGSGGGGGSGGGSGGGSGGGGRQSSDPQGPSHGPVPRPGPGPGQERDGERGRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVK9</accession>
    <name>A0ABQ5RVK9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_003859</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="11">
        <location>
            <begin position="414"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="446"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="833"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="156"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="242"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="497"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="669"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1028"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1096"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="414"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="364"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="497"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="519"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="669"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="722"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1028"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI61466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1122" mass="121050" checksum="B4D0713D0B39AA36" modified="2025-10-08" version="1" fragment="single">MTGKGPLAGGGGPPARRHKHAKISEAGTRDDATDAADLPIAKAMRKTPQVPKKGRKGTLPQPHSNGEVGDRGQAAIGNKAARSQSQVEVPKAEVPAKKKRKHGLAFWDSLPGWREIEVGDEFLLGAEEGGFAGLEVLENPVVLDQYMVEDKGLNEGGEVDDVHGDYDDDGGPLPGERDVFRPVNGSDGNDKFGKGKKEKKKNVSLNKDKEVNAVASSGVRDEKETAADTAAATLAVAAAAAAAEENKRRQRERMKAKKARRKQRRMQRPGAGAGDKGPDFGTKVNDAENLRSDVTPLVAGSKVNGASEPSAKWCKKGVSAVVVSAEHQHHQKQQAEEAGDGLSDHGKEKEEEDGDGGDKLGIEDGGDLDEDGDTEDLEYFDQEQEEEENQGEEQQGEEQEEEEKQDAQRKLDLSAWKEFELHSDVMAGLAACGFSAPTPIQRECLHPAIRARADIIGAAQTGSGKTLAFGLPIMHLLASERTAAGAVALEATAEEQADDMEEGATEEGEDWFPDREGAGAHAAGAKQGKQKQPMAARNRKKQKQSWPKPQKGPLRALILTPTRELALQVCTHLQAIGRPCGVRAAAIVGGISDPKQARLLAARPAVLVATPGRLWDLMCRPPSTDRNGPLAAAHLADLSRLSFLVLDEADRMVAQGHYKELSSILERLPHPANRRENTEDAEFAEMRKAKEKERRMKEKQQQGQKSERERRGGDPAERLDAVEEEGGGGEDPEQLQEEEEGGEDETEADGKQRGKKHLMQTFVFSATLTLPLFLKSRIRRGGGGAGGSAATLESLMDRVPFRTSPQPRVVDLTPQRRLAERVTEAAVSCLEKERDEVLYYLLAAHPGRTLVFANAVSAIRRVAALLKVLGLPAIALHAQQQQRQRLKALDRFRTDSQSVLVATDVAARGLDIPGVATVIHYQLPASADTYIHRCGRTARGLDGDGIAIALVGPREAARFIGLTRVLGRDPPPSFPIDQSLMPQVRARVRLAIRLDEIERKERKLRAEDTWRRTNAKALGLDVDEDEGAGGLDIDADDDESGRRLRNGRQARPSAQAMALRQQLAALLAEPLAPKMSRRYFTGGLAAALATRVKEPAAAGGGVAAHSTAADTGKVVEGTPTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWA5</accession>
    <name>A0ABQ5RWA5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TATA element modulatory factor 1 TATA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF40">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="245"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="360"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="593"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="405"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="842" mass="89568" checksum="082FCF2E3E8F77C7" modified="2025-10-08" version="1">MSSLAPRIAELESALRIVEKERDDLAADVERLCLQSGSSMFNPNYLLTERLNCAESEARQVRQQLTAAKEDRERLLEELQLARSAKISTERCAAELSERCKSLERDVTFYKERAVKAMAERDQSIEDLRADALRTQVQFDELMGSLELAQASKKELEVQLRSLQVELDQLRQQAVREQHSANAEGEAERRRLQDTVQHLEDSTQKLKTQLMEVQAEALHTHKLLKQAHKIEDQLSGQLRELQAELAAERRQRSSGDQSPQTSPSGTRSSPSSAGGAPVAAPTGATEDRFLRTSPGSRSPVATIPLHPALRVSTSSSPMSPSSSLAVVATRDAAKRAPSPRRALSASTTVTVTVTAAEAAAPPLQTSAHPDCGEPDQETPSSSRLERRSEQRETAGAAVGVSESALESLPDRARELEEMLAQVREELSRERAVHEDEREQLQVLRTMAHDFKIKLAQATQEKVEALLKAAQLAQTVADAQQHHNHNHNQVQQLTPGAGQPGAPFVAMQRQGSLASERVSRDFTRSPGSSSNLLPATSAPSPPAAGGGQTSARSLWATWLGGSGGGWYGSSAATVTATATASTVPITTASSTAAALTPPLPSGPPGAHVTGGGGLPSVTSEEGFDIDGVGAVSGAAAAASTAAVVATAHAATVEAAEGRSRRLAAQLERVRSQLAAIDRLTGHVGRCEARLRSCAALLWSSSEGDAPRRWAALGDLTRLSEECIALRTEAGLSSAASASASGAPPPAGPHFHDLPPVLPLALAEADALRTLPHLHLPQHGHVRNHGDGAVAMVASEYDNSVVCTREAGGVQRRVAEVLLSSLEFGVRSLEQYNHQSALSSVLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWF6</accession>
    <name>A0ABQ5RWF6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004533</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044239">
        <property type="entry name" value="FKBP20-2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47414">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP20-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47414:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP20-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="2">
        <location>
            <begin position="174"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="277" mass="29587" checksum="6CA62560CA57DCD2" modified="2025-10-08" version="1" fragment="single">KMSLQSSYLSDCPLRTRCARPFASRFRPAPSFVVLARARPDTTAQASSKCADRSSELVTRRHLLATLGSAYLGLALSPPSSAVAADGVPAPIGPPEGNAILISTADAEVLTSQEKLVLNINRRIQAQNRVPGEFPGFVREGFDVKVVGDGYTVASSGLIFKDFEVGSGAQPTDGQEVVFNYTGYNESGSVIDTSFRQGRPAQTRLGVGGMIPGFEEGIKTMKPGGKRRIIVPPALGPPVGPSTFFSAKQCEVFDVELIAVRTCARRQVMMFSDLVCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWM3</accession>
    <name>A0ABQ5RWM3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004602</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14364">
        <property type="entry name" value="CUE_ASCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052586">
        <property type="entry name" value="ASCC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041800">
        <property type="entry name" value="ASCC2_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494:SF0">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 2 ASC-1 COMPLEX SUBUNIT P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="474"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="648"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="763"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="844"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="649"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="670"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="710"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="780"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="853"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="895"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="908"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="925"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="939" mass="96461" checksum="9BF982421EF3498F" modified="2025-10-08" version="1">MSASPSKQATTPIGGQYLPFLPKDNQTWQADEVIRSINDDLAHLLSLPAENFWSTVGKDESLHVCLDTYLRYKRRVYDDFREEVEGASATSQQLARRVFMVLLRMVTTSERDPGAPLREEQAQMLYNLWLLDIPKLMDVAVLYGAHNPELTRKFLSQVFDLQPRYSDDLAASAPLLVGNLAEVVGRCMTAGDRALKSGPAAAAAHVKELRDAMDYLRDAILTLTAFVACYSPAATALLQPDHGALLCTLASVHDLLLPLLARVFSQPTVQLLNQQSGKKGPAEQGPAAAGGGNSGDDTQGKLSHVAAGLRRLGYLLLLSSSCRAVPQGARRENKLVQQVSEVRGNELMSLLMAVSSAGTAGRGGSRGEDGSSAVGGFLAQINRESHLDSQIASAILEGTIALDDAQFDYLLALIGGDPRVMDRVRNQHRQVGPSSVSAPSTSGGAGAKPGPSGSAGAGPSGFGAGTSDPAAAARRTSLISQIKDLLPDFGDGFLAACLEVMDMDPEKVINALLEDKLPPHLAKMDRKLATWGGPVAQAGSGPSSSKMAAGSSDALADMSSWTSLAGLASTSIPTSAAAGPGSTAKGASSSGASFWRTAAGGGGVGDGSLHRGTLKVLDRVDKEVRSLTSRLGDELQWEYDDEYDDSFDDLAAGGADGVADAEGDDEESAMGRPIAAPRGAGAASGTSRLGPPGRSPRETSTEYNDESYRRTASSSADAGTAASGSSSAGSRGTGKPPANASVSTGAAAAAAAAAAAAGIQLGGAPRRAPTMTGPATSSAGGRGGGAGPAGGRGGRGGRSGGGERLWVLDGKIYNYAKEGAQMVNSHQEADALLDAARQAAQAIYGLGPGGNKGLLRQQKQQQAGDGGDSSEDEGQGAPVANGRAGGAHSGHRGRGGGGRGGGETGGGGDRRQHDRKEKNKAAVANHHRKDRALRKQGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX71</accession>
    <name>A0ABQ5RX71_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="111" mass="12629" checksum="FB60ADC577A9DDFD" modified="2025-10-08" version="1" fragment="single">MDPELRTTCEGVAHIRQLVAQLETEAALRRGDKLQVEMDEEKKQLSSFAAAEQQCKLQFADSTAQIDDMRKQLQRMQSEKEEQIATVRREAAASGAELEALREQLRLAQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX88</accession>
    <name>A0ABQ5RX88_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="435" mass="50251" checksum="4E7507DD83D191EC" modified="2025-10-08" version="1">MDYTILGPQRKIMAQRASHIITTNDLNRPYRDIVKHRGTWTMEDWLHWLDSYSVYILHEEDSLQSSTPLMPVVQRVVDGITKSFNIKVMWNNLRTALLHYFRYDPSDFSADANNHAAGLLKSFSIAAEHVFGIQFCTYNLHILVCRLREQENARGHVAFATEFWVERGMQMMKSIVKYRSPTVPELVLAGGYLASLWLTHLKAAHPETFRTFDEWVPGYRADNIQLNPSKRDVFLDEEDSEGSQLLGQGYIADAEQRSHILGGILRTEETEDFMARTNLLPSVLQDSDIYIFKRALKRRDEVVLSVDYARATKRQSYHFKCMYGPGGMVSESDPRVSIFIGEILFFALIRPRCDVAPVKVAVCNLHRAEVLRPKELYRVKDYSRRDATVNEVRQEILLNYFPKEVGMIDSKVIRCRVNNELSFPHICYLLSASDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ54</accession>
    <name>A0ABQ5RZ54_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">sulfate adenylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-sulfurylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Sulfate adenylate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ATS2</name>
        <name evidence="12" type="ORF">VaNZ11_005613</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43509">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43509:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="11">
        <location>
            <begin position="39"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="10">
        <location>
            <begin position="202"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI62875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="419" mass="47108" checksum="FC07CEA155E9DE88" modified="2025-10-08" version="1">MLQLCAQRFQGLLGKCAAYSHRLYSAHPAFQADGTGRILPHGGELVDLLVPGSHEKMDLVKSCKYTQECSDRNACDVELLSVGGFSPLKGFMNRDVYEQVVANMRLPANNLIFGLPVVFDTNNERIKEGDRMLLKYKGQDIGVLEVESKWVPNKARETKECYRTSSLEHPGVQMVSMERGKYYLGGKVWGLELPKREFPCATPRVVRAMLPADQDVVVFQCRNPVHRAHYELFTRALHAPNVRPGAVCLVHPTVGPTQDDDIPGIVRYHTYEVLKAELQNPAIFWAYLPYSMHMAGPREAIQHMLIRKNYGCTHFIVGRDMAGSKSCLTGEDFYGLYDAQDFANKYAAELGMQTVPSADVVYTEERGYVTADIAKKEGLHVRKLSGTKFRQMLRGGEDIPEWFAFKSVVNVLRAHTQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ61</accession>
    <name>A0ABQ5RZ61_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="276" mass="31121" checksum="4E2807E375E7A730" modified="2025-10-08" version="1">MSGILALVALTAAGAVGIRRLIQLKRRASSTTTEPDPVTPEAADRSVVIVDNGGRPFVVRLSYKRRVATVYRTRWGEAVWECSSSEVLHSFRYRRAFVGVDPRERKMDAEEKRAKKKGNAAATAAADATTIDAAAPAAPRKIWWFGGTSVLLDLGRRRYVFIGWVIYTFTAYDDIVDYVGTMGNSHVTYPFAVGKKNTYLMIEMTYIPNHILRTCRADWLEDPYYVLYESDLSPWIPGMVSKELGDKPKCPGTYLASWVKSFVLKDYTLLVDRQWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ69</accession>
    <name>A0ABQ5RZ69_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">hydroxyethylthiazole kinase</fullName>
            <ecNumber evidence="4">2.7.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">THI10</name>
        <name evidence="13" type="ORF">VaNZ11_005776</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-(2-hydroxyethyl)-4-methylthiazole + ATP = 4-methyl-5-(2-phosphooxyethyl)-thiazole + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24212"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17957"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.50" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01170">
        <property type="entry name" value="THZ_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00228">
        <property type="entry name" value="Thz_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000417">
        <property type="entry name" value="Hyethyz_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006830">
        <property type="entry name" value="PRK09355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00694">
        <property type="entry name" value="thiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02110">
        <property type="entry name" value="HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000513">
        <property type="entry name" value="Thz_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01099">
        <property type="entry name" value="HYETHTZKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI62925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="286" mass="29552" checksum="247D3B12E0B02409" modified="2025-10-08" version="1">MSSSAHSDNSLKLEATSVSCWQLWQAVRSKRPLVHCITNFVSMDFMANVLLAAGCSPAMAHSLDEVESFVAISSALLVNMGTLSPEWVASKKLAAKKAVELGKPWVLDPVGCGATPVRTTSCLELLACKPTVVRGNASEILALAGAAASVKGVDSTAISNDALEDAKHLASKHSCIVVVSGAVDYVTDGQRVVTVRNGHPLLTLITAAGCSVTALIAAFVAVAPGQALLATAHALAVFGLAAEEGLKVVPRPGPGSLRVALIDLLHMVQEDTVVEGVRVAVMSQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZK2</accession>
    <name>A0ABQ5RZK2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDEase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005885</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="4">
        <location>
            <begin position="338"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="199"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="298"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="221"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="512" mass="54390" checksum="36B3CD94779F43A7" modified="2025-10-08" version="1" fragment="single">MQRSYRLVARLVRLLVDLKGGTRVGARTAIAPTQNAAAGGAATVGRGIKGTTSQSGAIKDLTTGGRTWWSHFPKTPQLPAGAFSDAAQSCGDQLSDMGDHIEWTGTNDNEDLSYLDSDLLDLDLMAMRTDTPADVLLSILADMLDGREPDLREVALARSALLRHMDLYEPLNSGGHIRSAGLDADVADALMHQLGASTATANSLSVDSSTASSGPRGTTNPSRSPSLSPTPSSSSSSSPSLSSSSSSSCSSSSLSDAKKMNREGGDGGDDDDDFDERLAAENLKDALAVLLGLAAPSPPLQQRRSSLVEEDGATTSSSSWGSGSDSDGNLQSRGSGVVPEPLIEEVERMLALAYDSWQYDTWRLADVTQGHALSCLGFYLLHREGLISQFRIQPTKLAKLLRTLESGYPSNPYHNSTHAADVLQTLHMLVRGAGLTPHYLDPLGLMAAYFAAIIHDHGHPGLTSDFLISTSDPLAVRYNDRSPLENHHGASFFALMQQPGMNVMGQLTGQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZU4</accession>
    <name>A0ABQ5RZU4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_005786</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04673">
        <property type="entry name" value="NUDIX_ADPRase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43736">
        <property type="entry name" value="ADP-RIBOSE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43736:SF1">
        <property type="entry name" value="DIHYDRONEOPTERIN TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="72"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="286" mass="30429" checksum="3D7BC148C54CBE36" modified="2025-10-08" version="1">MWSLGSSLHLTRRVLSSCRQARRALSSLTTSQRHINRYACYLLQQPADYRTSSTTATGSDSCTGKSRAYPTEPRVGVGVVVFRTQPVQGRDSEVLLVRRAKDPDKDRWCFPGGSLELGETLAACAAREVEEETGLRLCGGVYGTAGGSGHVSVGTAAPVTAVDGAAQGMTSTSTSAAAVAWASSADSWSLTNGPIPFAAVDVISRDADGRIQFHYAVIEVAALCSGPDAVPISGDDADGVQWVPVSRLRHMPDLTVRCDEMVEEALRRFAVADSRRLGRPNSEEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZX2</accession>
    <name>A0ABQ5RZX2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="623"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1185"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1213"/>
            <end position="1228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1247" mass="132297" checksum="EA34B3D34AA4360A" modified="2025-10-08" version="1">MLGGWPYQNVNNCPAPKRTASHVNPLPVRKPSKRAKYGDGVETNKARRLARSSKAPVVRPKPRRALQYFQELIQRGEEGHRGGQQHEQQGGEEEEEEEENKQADQQAKQQQGHQGQQQQAHQTHQQQQGHLQPRKLQDGQQQQQGQLEPPGHQWNPEMSPSRDPSKSEARRESGDEKKIQIQYVYPRDEDPDLDPDPSTHPDLDLESDISAPTPTILGMVTPSPAGFHRRRKLFEDSPDGPYPRMTAKIVARLAACSHWRRPLAHRLLPKYLPLFDEHAAAYFFKHVPSLQPRPVMPPTSQAFEPSSLKITGGGGSPTARAGGALNRDGGATAVTSTASRRGSSSSSSSSDVAGFRRMVVAVCCSLLPKMPSFGPRALSCMIAGLGRMGIRHGPTLAAWEAQSASQLGLMRPDQLVASLEGLGALQRSKTISSVALGVHSAASTGGRHQRLRRRRPPPSSSSSSPLPLVVGDRWRLAALAAVHSALPHMTVAEVNRMLMALALLRLSPSMELLSEACTRVRNAVTAEAARLAAISGVGSFANANNDVHNGGGNSSDRGDGAVAAEVQQEQRLQTALLTIASALEESAAVARLATLYRHGMVGATGGAVGIVGTSASAASAASANARPCKGAGKPKGAGRSPKKQRVKAVQLPEPVRARDIAGVVKGLAALGYAPSRDWLDWLVRNVEVVLLAELKRTRQQPQQQTSPRGRSRRLVKADAAAAALSLLEGSIPAPLPVGAPGSAVATVEAMVGTPLPPYFSAADLAELMSGLADLTSVMLQPSAEMVSTSAELVGGGAAEGAPAAARPPVPPLPRPLPVPAEWLNLLLDAALEDLRRRLDPGSAVAAAAAAAAAAPTALLTQGDITKADATLVAAAFGTSNAAAVAAADAVPAASCLLEALMKLQPYYAPPRHWCMAAAALGLELVPYMQLSLLLLWLRSIAAWDVPRPLTPAWHAAAAVCSRTQLWYCTTKELLDLPGVFAAAGVRMPATWAAQYVRHLAFGLRGLRGIASSPSPDGARVKESVSPAWRRRERQLQLQNRSRPPSVRPSWRQCGDGISVGSSGDTIANIAGENADRRQQIGAEVESPEQGSMALHDVATALRWLPYIVPDLRYVAECEGDALQALARAFGLLLLQPLPLSWSSTSSPLQVSPPLSMISETTTAIPVPVGTLPAWSVSAGSATVAAPGVRSTGGRTQADHERMRELRRRPRTGVSRQRSAATTDMMSSPQAREAVRAFLGAAGISCAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZX5</accession>
    <name>A0ABQ5RZX5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005821</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47869">
        <property type="entry name" value="OS03G0410700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47869:SF2">
        <property type="entry name" value="OS03G0410700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="103"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="337" mass="34650" checksum="C3DEF8A16ED592E7" modified="2025-10-08" version="1" fragment="single">IVESLGVSLALCAMLSNFVRTIGHRPTIISKGVTVSPFVRYRNIVVCKNKIGDELLDFVTAGPKLRKWYGAGERPVDGGEEQEPDEPDTMSDVVLVTDADSPIGEQVVLQLILARQQLRLLVRDLAAAKTAYGPYATPLPTDAGTSLRALRGVKCLVLLGRPVRGLIEAAKAARVKHVVLLSSAPAAALTKSSAFSLGGFSLTGGAGSSAARELRELGDSSAEAALKASGLPYSVVQVYGLQDSGRSGSGADGVVLEQQQQVAVGKAAGSNGSGSGSAVSREAVAALLSGLVDAVPSRGRTLQAAGSRQAAALSPTSSPQRQQEALVSALQGIVEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S005</accession>
    <name>A0ABQ5S005_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribophorin II</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ribophorin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_006114</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SWP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055374">
        <property type="entry name" value="Ribophorin_II_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056790">
        <property type="entry name" value="Ribophorin_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23860">
        <property type="entry name" value="Ribophorin_II_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25147">
        <property type="entry name" value="Ribophorin_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047204857" description="Ribophorin II" evidence="13">
        <location>
            <begin position="23"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II third" evidence="14">
        <location>
            <begin position="53"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II C-terminal" evidence="15">
        <location>
            <begin position="191"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI63225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="296" mass="31176" checksum="0E8C312932FC50FA" modified="2025-10-08" version="1">MASLRLAISALVVQVAFSLALAQDAGGLVSELVLGNVKVVIKSMDGSAITTLTAAYPKPVLANPEMPAGASLDVTISITKGGEVFKPQQVMLMLKSKSLGLAGYAVGKFKSGSYALSITAAALEKQIGKLPGEYEVTLLIGDPSANGVAYVLGTVELRISAASTALEPTAVVRTAFFQPVNNLKPEIQHVFRAPEKRPPVVVSYIFAGLAFVPLVAVLLYVPTIAGVNFKGWTAAPLYSLLFHGGLGSMLLLYVVFFLRLNLAQTLPLAGIWGLFVAGSGYLLLSSLSKQRLKKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0J8</accession>
    <name>A0ABQ5S0J8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004710">
        <property type="entry name" value="Bilac:Na_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361">
        <property type="entry name" value="SODIUM-BILE ACID COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361:SF30">
        <property type="entry name" value="SODIUM_METABOLITE COTRANSPORTER BASS6, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047439741" evidence="2">
        <location>
            <begin position="18"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="380" mass="39549" checksum="C0DE4B19DAC0945E" modified="2025-10-08" version="1">MWQVLFALALTAGVAVALLAPNYMDNIKVDLTHTLGTACFFLGISSTAAKFRDLVGSPLGLGNISFSFFIGTFLWPALGLAATVLVLQAGWPFAVAVGFVLLAACPVGGPSCAVNLQTAGGSLECSLLMCLLSTPAALLTFPYIYRHFILMLLDQPLSPVESAAEARFRSQLSPDITSSALASLLHHVLFAAVLPLSAGAILVALLPPKLAASARCWGSAAAATMIVLTATVKTAEGADTVRAAGAKVAMAVVGLHTIALVLGYLIPAGVSAPDMTCRTTAYQASMRNSALGLCIAVALFGSSEHLALVVAPCVVSVFTQNLLGAWTALLGQLWAPRHPVSNSGQPVSPQAFKANSMDSILRQQTFRRLSERSSINAKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S140</accession>
    <name>A0ABQ5S140_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="662"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="690"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="558"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="994" mass="104939" checksum="FD03C5BED62E0C95" modified="2025-10-08" version="1">MAPGLDILAAGPLPQRGFGDPYWPAAIGDNVAYIQRTCVPLPYQLNSSGSIPRPVGYPGNQTSEVNLPQSACTQPAPTNNSVAASLHPMQRCWPMVGLYEDLATFAFNLDSLSRQAAAGYLVVIRHSQYFCETLMSDDCAKQYTPAGCFLRMFPRSSSNSSTGGTDEPPPISTTRPPPSIMMTSVPTIGTADSKSPRVPLGLVLGATLGGTFVVCLAAAIIAFIAVKKLRRSHYHHQLQQQQQQHGGGAPVCSWCCTLLLYHRNFCQNVGCCGGNKNFVPAGRDEADTVIDLKGRQDHHGTIITAQLSTAGKDLSSSVVPSIVSGSQQKNFGALASAASFRIESSPDPLSLVPVTSLTPLHPGINLDVKLDGGELTLSGVTLGRGAFGRVVKGTYGGVPVAVKLIDHGLGPSPRQQQQQQVKQQEQQEKQQQPQQPLLHLQPGADPKYPGYPAGPPLAATQQQHPAASPPVLQVPPPALATGPTADGVVAAAPMAEALPQTAAPLIPNVTVSLSAAGAGGEGACGSQPRVNTGGGDGDAATVVVQQQLETQGAGPRRPHGDVPRGEEAPLSSRPEVQQQEDHRPIAAACQGDEVIISHASTAAADAIIKHVMAAAAVSSTGSRDDDPRSRCNMLVPLSAHVRGGGAGGGAGVEILGTDYVTGSKTTTITAGDYTSTSSAALVDSSRDANLHGDTQMLPLSSTTSTSNPRSIRHATGEKSATKGGAVPAVVKGLPQQPSAEDVPNSLQATLKQEVEVLARCQHPNIVCLHAACLKPPWLCLVMELMDTSLDRLLYGEKRLLPLDTVLHIAIQIARGLSYLHPTIVHRDLKPGNVLISNASSSRPIVKLADFGLSRLRNSVLITKNPEVGTAPYIAPEAFESKNFTITDRVDVYALGIMMWEMLSGRRPWTGHNMVVIAIVVAMHQRRPPLGLLSDERCPPKLRSLITACWDPDPVRRPAAAEVAKRLTLVQEALNVGERLTESPPRKEHQGPLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1K2</accession>
    <name>A0ABQ5S1K2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.810">
        <property type="entry name" value="Photosystem II Pbs27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01481">
        <property type="entry name" value="PSII_Psb27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025585">
        <property type="entry name" value="PSII_Psb27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038450">
        <property type="entry name" value="PSII_Psb27_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34041">
        <property type="entry name" value="PHOTOSYSTEM II REPAIR PROTEIN PSB27-H1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34041:SF1">
        <property type="entry name" value="PHOTOSYSTEM II REPAIR PROTEIN PSB27-H1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13326">
        <property type="entry name" value="PSII_Pbs27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="178" mass="19384" checksum="15BED4DAA7631FD3" modified="2025-10-08" version="1">MASITCLPSKVVPVLRARSSRYASPRLSVVASAQREAPASRREVLGASFAAFSLLLSSSPAHAIFGFAEDDSKLFADYNSETSAILGKVKVTLALDKDDPTKEDTVKALRKDINNWVAKYRREPKVSGKPSFGNTYSALNALAGHFNSFGATAPIPKKRLERLQKELDDASTLLTRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S208</accession>
    <name>A0ABQ5S208_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006304</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823:SF9">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47823">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="348"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="400"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="428"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="546"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="862"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="59"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="132"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="649"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="734"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="152"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1015" mass="108597" checksum="1B0AFDC859E2C309" modified="2025-10-08" version="1">MNNATDLQLSSVGMADSHFSNSSNYTSSTSLKADVVHQPPDTAKAHRGCQGKLPTLLATTSQSSQTSFRRSRIIRARPLGESTELQSSDDDSEDEVKGTTCIGTRRSTCSDGVARGWSLFVDAASRFIQKTPSDLDGDLEPGTDGNNINVGSDGGGGGGGGAGGGGSSAACATSDPDRVSFTGGLRRLLLVAFWDILHPESKFRVGWDALVISCLMYVAFVTTYLLSFEFHRNERITFFVVCDLVINSVFLIDIPFNFRTALRRGDDAVRRRQVITCRRTIALNYLSGWLVVDLVAALPWTYITRSLASYMQLLKLLRLVRLLRLLQMSRSMQRNRLYMNMEHSQGAAVARVVSYVMGAVLVVHMSASAWFFVAVMQSKDLEGTWAEAAGLQDATAFERYLTSLYFVVITFTTVGYGDVYPVTTAERFMTCVGMLVGVVTLGYIISTANTIAGKADKYELARSELLDRVESFLEAQPLTTGLATRIKNYFEYVARRRFNDRDDQQLVASLPSDLRRAVLRAMHSKLVKQVPFLRAAAAHHEALVLEITAALKLEFYTKGEVVVSEGGWDSELYFVTEGTLQVRQYTADWGVGPAATAGGLRGPTSPTDTNGDAGLRLAAAAVSDAMPPPQAGIVAVRGSVAAAAATSAATTAGDGDGGSDIGDEVAPGNVTAGCSFRSRGGGGGEVEGNPRRSPTNFSMVRKWLATAVGGVTQAQPSPGDVVLKLYGENAVSAHTSNRDDGARGEQLATAAASPPPPTPPLLQVKLGAAPTDGRDQRFVRGSGSGFELGSVPPCSSGRDPGIHIGASSGVGGGGVGGVSVSGHVVGGGSFAAEELQPVMSPMQQGLWKSRSLYDSNLRYRRLKELKKGHYFGEYGCLTGCPRTATVVAVQMCEVYCLKRSDLKAAMSKWPGLEAAWKRCEEYGMRERDRLRLCLQANHTQRSLALIAVEDPYDNRPHDRATNAVVNSEKRYQDFLGHFGGAAGVIAAAPGRGTAAAESVAAVMQRDGIRSEPVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S238</accession>
    <name>A0ABQ5S238_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019002">
        <property type="entry name" value="Ribosome_biogenesis_Nop16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13243">
        <property type="entry name" value="HSPC111 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13243:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09420">
        <property type="entry name" value="Nop16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI63947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="198" mass="22479" checksum="183BB46B2302C36A" modified="2025-10-08" version="1">MGGSRRKLKRNAPKVRNGVVKRKSDKKTKIPLDIAFGNPALETRLNKKPEWSEKDTLNSNYSRNRLVSDPNVLLGRKEKKTPLVPAAARKEAGEETFSDDDEQRAALHLPRKSGKKPPARPTATQRKVVGALLEKHGEDVHAMMMDVKLNKMQHSEGELRKLIEAYRFWGPNCSAAQKHDFWTEKKPPKKLLNCRILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S294</accession>
    <name>A0ABQ5S294_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006959</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="204" mass="23566" checksum="DE7919F2F9CB702F" modified="2025-10-08" version="1">MDRMFRPFIDRFVVCYLDDILVYSKTCEEHLEHLRLVLDVMRREQLFAKCAKCFWSQPQVEYLGHIVSATGVKMDLRKVAVVRDWPAPQNLTELRKFLGLTNYFRKFIEKYSIIAAPLTNLTRKGVFVSLESWTSQCQRAFEELKRTVAEDVTLAFPDYLLPFRVEVVSDASVYGSGAVLLQEGRLIAFTSKKFSGAEIRYTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2D1</accession>
    <name>A0ABQ5S2D1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CSE family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012416">
        <property type="entry name" value="CBP60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31713">
        <property type="entry name" value="OS02G0177800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31713:SF96">
        <property type="entry name" value="OS02G0562300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="680"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1162"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1286"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="633"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="768"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="782"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="811"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="826"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="853"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="944"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1069"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1120"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1165"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1305" mass="136381" checksum="77B003382609DEE6" modified="2025-10-08" version="1" fragment="single">MYTRIRVPAELKPTPPQVLEDYVRVRITQFISHFCQRLGHYAPGLAKETLDRAAAEALAYLPPGDHGQLSLEDKQLQLVFRHDCLALGCLDPQCALCTHNPSRRCMVNFDRKYLVNDVLKARCGAGIRVELIDPLTGQPVEEKLPEITLQISVLDGNLYDNKFLDSGKQYEGGTEPAEELDACALLQNKKKSTALLVCGSGGSNDSAGYVLLTMVEGKVPLNDLHVTDSSEAILSGRKPPFRLLVRALLPQNGPRLNIRHAVSEGFVVATRRTRTAGKADIPSVDDPVSKLEHMGKETVKKLADLATAAEQAGVEIDIPENCVQKVGEFKMLALRADQDGHLRQKLQHVLKLSKEKWDEARDHAMRAVVADNRMRAWYADRRSCELGVLFTARMGNVDLDRPVGLLQTRVEAGERKTEAILVAQLNPTQREMLRHMQPQAVAAWWAPGHTGWAIFPMDSDTFLQSGTLTAAAAPPSALATAAATAAAAVANVMDAPPSPTQSAVSLNTMPMRRQTGGSGGGDTGGAGQSGDVVALATESSLMGKPMPLPSGLPLQVLHGGPGQGLNSGGSLPSAFGGSMPSAFGCGMGSGRSSRAGSMTDSPPPSMRMPTPVSMQPPQSLIHHHSLPQPPQSQPQLQPHTSLHSQQQLSQVQTLPSFQNLPSLQQHPSLQAHPSLQGHLSVQQHPSLQGNPSVQPHPTLQQHPSSQGHSLQPHASLQGHPSMQVHPSLQGHPGMQAHPSQRQGGPSGIGSGRGPSFSAMPQSPLAPTGGDGGAPGGSGASPSAPPHMMPPPPPAMSASRSMMMPPQPPAPMSFRMPPQHPQQHQQYRNQSSEQYQHGQQIQQMPQASTIPHMGTQGQHGLHGAGQQRANGSGASQMPPPPPPTHAQHNPGMQRIQGLDTQSALGRGQMPRPSHQLVEDSDLDGGPPPLPSTNMRMPVSGPSFGPQHTQQQRQHSVLSHPLGRGAEDPPEDLQSRGSANPRPHKMPSLGRSLDIHYSAIIVEPPGGMKPDYITGSGGGGSVCRGGAVNTPPHITPNEVGSMICKEEAAAGTPTGGGAASGQAPSPAQRAPSPPRGIHPGPRAPQPVPGQVPPGATLPPPGPPPNPFARVQSGKATTMATAEDTDDDDSDDPLLAPLPSLQLINSNAYHATLGGAGAATGTTLRATGHGGNGPSGGGAAGGGAGAGAGGAFAGPSANGGLNAPLPSLHMIDSQQLDMVLGEHMILETSSLNTNLSYGLFLPPAMAVGEGAGLLPGTQSKELLKPTTSGLDTMASMELALPGVRKNEPHALGQQEQKAAGSAGGAVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2G0</accession>
    <name>A0ABQ5S2G0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053209">
        <property type="entry name" value="Gramillin-biosynth_MTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47643">
        <property type="entry name" value="TPR DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G12710)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47643:SF2">
        <property type="entry name" value="TPR DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G12710)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="67"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="540" mass="58140" checksum="C58FB33A2F817F6C" modified="2025-10-08" version="1" fragment="single">VENAVVILSVDLEKAKAMRTVRTLCPTQSGRRLGAVIVRQCSATFTDAAADPVWLDNLYCKAALTPPELQRAVHVAHLPSKGRGILANTDLEPGELLMVSKPLAYVTCPMGSIPSAEDLITVMKKSNYNTEELRILNSLFSGVDDEDNKDDTCSGLNLDDFKLSGIIGCNSFGEEFTDFPSYLARSHAHANGIDGNRNVVSGGAEHDTASWSNPPSGEEQVGHLGIFPSFSMLNHSCLPNAVNFVVGGRMLVVAARSIQKGSEVLINYLGRASLRPLEERQAQLAEGYYFSCDCPRCRAELYYCCTDVAEASGTTNPFGTLAECLQDFLARVDTRNSVLDQLGRAAAASVTTNDDGGDARAILDGLLDSVRQDVRKWDDHVQRAQPPLAAVLDEGMGPEVPQSWLHASAFDLYSQHAVLAEATGRRQEALAAVERQVAICEVVAPSSDLHMYLSVKLALLAQQQEQAEGGNGGGRDKGLVAGVQGQWEAKCGLEGGSDRVAAEWRRCRAVLQRRYGKGLSDGTLSTLLQEAVYGLSQVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2H7</accession>
    <name>A0ABQ5S2H7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CTP synthase (glutamine hydrolyzing)</fullName>
            <ecNumber evidence="3">6.3.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006954</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="53"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="314" mass="35283" checksum="D87913EA6039FFA8" modified="2025-10-08" version="1">MQEQGAHQLLCAKLGLTEADKLDLTSWKVNLADRWDSLTDPVKITLIGKYTNLSDAYLSVIKSLQHACMEARVKLELEWVEAASLEPESKESDLTVYNASWRKLVEADGILVPGGFGSRGVEGKILAANYARIHKKPYLGICLGMQIAVIEFVRNVLGMTEANSTEFAPATPHPAVVFMPEISTTHEGGTMRLGARRTVLQTMSCISAKLYQQDQYIDERHRHRYEVNPDLVPKLEEAGMHFVGRDETGERMEILELVDQPTDHPYYVAAQFHPEFKSHPGKPSPLFLGFILAASKRLDRYLRSRDFPRSATQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2T2</accession>
    <name>A0ABQ5S2T2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045868804" evidence="1">
        <location>
            <begin position="20"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="145" mass="15764" checksum="D4CE0B431477E2A3" modified="2025-10-08" version="1">MKMLTLLATLLASATIVHSAEFTVKGALLADRNATADYSCAWPRGGRRCCGTLRTPGVAHGWRDDETDHCFPASASDHMVCCVDIQNVDNEFNVDDPTVARHNPLSGPIRRNSNPGSYSWCTCSATICEKQLKGRVAWIGRPGQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2V4</accession>
    <name>A0ABQ5S2V4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007046</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF194">
        <property type="entry name" value="INVERSIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="231"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="264"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="298"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="438"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="471"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="543"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="739"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="810"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="843"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="907"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1085"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1171"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="914"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="950"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="996"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1009"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1085"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1171"/>
            <end position="1191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1203" mass="122257" checksum="1A5D57FE1D81B44A" modified="2025-10-08" version="1">MPQAPYRGARLISAAQGNSRLGSSSSKSLDLTWDSVPRAVTENEVRLIGLVREYSAGHEDARVIDSIFELLYEETSPNVMDADAGRSALHWAVLHGQTQLVRTLLEAGALANTFAKDGFSPLMAAVRKGSSEVVGLLLDEGAKIDLINTTTGCGPLQLACVFANTELVQLLLDRKATPDVRWNGAKGVMTPLVAAAAKVCEPKPMAELYLQVVDKLLAAGACPDVIINQEKGETLLHSAVARNQLGLLRALLRANASPDMPTKDGVTPLMTAVRLSSAELVEALLDGGAVVEAINEYNGKTAFSVACNLGNLELVQLLLARGASVSTRWFNPNDVNAPILVVAYKCDDSIRRGFVSPSVIRSFGSLSRGHSVGSGNATAAAAAATAVAAVPGPSGGSRDCSGRLTPLTPRHAGTQIYLEIVRVLLHWGADPNTQGSKNGTTPLLIAVENNHLGLLRVLLEAGANPNLPDTNGCTPLLAACTEGHLEMARVLLEHGAKPNMQRRDGWTALMGATLNRSTAMAELLLAAGAQHSVLVPPGELLAMESTPLMAACGEGSLELVRLLLAAGADPNVANSAGQTAVWCACRGGQAEAVQELLTAGAVLRPRGQNLILQCGQLVGGRFTPLAAACSRGRGATAALLLHRGAQPDVCWTVDGRAGKVELRPLLAVVSHPWSDDPEGDRECAALVEMLLAAGARTEAAEPGARCTHNAIEVAAREGRSGALRALLGYGAQPNLVDKDGATPLILAALKGSVECVKLLLAAGANANATTQVGVSPLVAAIGAFAAGDGNLELIRALLEAGAAPNAPFFEEDTILTAAVEGGNPALVSTLLAAGADPSMPNTAGALPLQLALTKGHSELVRLLLQAGASPHLPNVDWASMRYHQKGRHEILELLAAAGIVVPPPPPLEARSRGCRSCSTSGATPTSASSSGRRLQTRPSSGEVRRRSSQGGIGSSGLLGGGGSSGHSSGFNGSVYAGGSGGNVPSGTVRRKSSQGRGSGSSGSFGGSSGPRSSVSASGSLGRPSGNGFLMLDTSASGMGLGPPTNVSGNGAVNCRRPPPLDLGRTNSCSDSDEVSVGCNTWATSAPASSSGTALSPSMCPESSSSAPPTLLRNPDGSPAFERSCLLLLSPTPTGGAAGGATVGRGTGGGGGGGESNRGGLCSTATCGALPSLNSNGSGRTDSKPCTPSPAGSGTAPVWGVLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2Y0</accession>
    <name>A0ABQ5S2Y0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006721</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12007" checksum="E4800F88205EE103" modified="2025-10-08" version="1" fragment="single">DELTATEVNGGCSIGWRNSGYNDGTTVPVGLRVMDVTTGQFNDLTFLVMVVRSNFLPVVGKITFGGMTLPQNGGSVTMYVGKQSQVCISWGAYIIPVCSFHYTAYHDEGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S339</accession>
    <name>A0ABQ5S339_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_007549</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="789"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="223"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="717"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="857"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1266"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1179"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="287"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="473"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="737"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="865"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1274"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="817"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI64322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1365" mass="145035" checksum="D936CA2BDA1F5231" modified="2025-10-08" version="1">MAVLMPECPGALLTTEGEDKPRIRVRLHFGGDFAQEGPRQWRYVGGEVYNESFPVDSKYAEVVKRLNDKFGDTVSFKYLCPGDDMDPDNLVQVQGDDDLREMCVEYQNALQKTSTPVNTVRIKVFVYRAVIFEQEVQELDEELGEDDELMSNAGVDDLLSSCNPVENGPNWSDWGDPDEQYVTHSSVRSVQFSSDAERHASIAPAAVMTLPYITSMSVNSITSAPAHSSVPPAPLTSAGGMGGAGSKVPHWLPQMKQPSSGALAHPPSQPKRLSAHGASGEENCGAAGNGSGGGSSSSGGHVGSSRPHHRGRQAVAAAAVPVADPFAFGGASGAYVPSDIDVLAELSYARGYGAEGAFMDTEQLEQSGEELDYDAIVLEDGGEEVVTAEKAGGAGGDEEQGEGVWVQPEDPYNLVCQPLSQEQQRSLRKGPPEYNWMEAQGEARDPVQGGWRHDCECGLEWSRLEQQQTQERQTQEQQERTLEQQQQMQTQTQERLNLKKDHHHRAVLLQPGLGFYSDGELEDPYFNASSADAPDLRMLCVTDAGGAQNCNSAASDVALRRLVQRAGDVAVCDEQAAGHVGPSGVREAGTWALLDLLPRHISAFGESSRDDSKSGGGPLLPSRITIFGDDGSLNGTPRSGGGNLIGMGGRHGLGELHRLALSGGRIGPPAARWPYPRANADDAVIVGPCGVDAGMLDRVAEDLMKSVTTFFYTQEMQPTSTHEPGTHGSGGEEKMGGGRGGGGKPLGMGDSLSRPNGPDIGLGGMDHISSATGHSHLLKGVVKKRKSEVSILAKIGEGAFGEVSQAQVFPYGIVAVKWLKRERFAKYSESFQREAETLAKLNHPNIIRMFGLVMDTPDSPSSGGASSGGGGVNGRGAGQAAHGGSGGGSGGNNNHGGGNSPSGAVTPTGPIGGIITEFVRNGSLGQYLRSLNGKRLSLRQRAMIALQAALGMAYLHEQAPAVVHFDLKPDNLLVDGEGDSMVIKVADFGLSKHKLSNYVSCRDLRGTLPYMAYELVSGNDNVSEKVDVYSMGVVMWEMYTGEVPFAHLTAQEILTGLLHGNLHLAIPPACEPEWRSLVETCMDPNPANRPSFQELVMQLQEILRLERQASNASSAAAAAAAAVLDSDTSVAEKLPSDNTAGWRLPALAATPVVGAAVGSGLAQVRSPRAGPCVPQPPPVQAAQALLARQQAQMQAAAAAAAAAQQQQRPLSPPLQQQRRHSPLLAPLQQHAPPLPQHRHAQPFQVQHQYGNLQFALPPQAACQNLSQVQLPQQQPGPPQPPPPQQQQIQYLPQLTPGLILQQQQQQPIPVPPAHLYQLPLRPQPLFQPPPQQHGQHLNGLYGLVGGGGVSGGGLPALVSWGDRGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3B1</accession>
    <name>A0ABQ5S3B1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="2">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_007261</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000848">
        <property type="entry name" value="PRK00074.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54810">
        <property type="entry name" value="GMP synthetase C-terminal dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6 11" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="12">
        <location>
            <begin position="209"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="237"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI64089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="536" mass="59582" checksum="C39F5E46D9B933AD" modified="2025-10-08" version="1">MTTNGTTSHHAVVLILDYGSQYTQLITRRVRELCMFSMLFPGDASLDRIKSASPHVVILSGGPNSVHVEGSPRVPEGFFEYCAENKIPVLGICYGMQMITHLLGGEVKPAVHGGEYGRMPMDIAPSSQLFDFISSTTVNVWMSHGDEAVKLPDGFSAVARSHQGSIVAIENPQRQIYGFQFHPEVMHTEHGLEMIKHYLIGIARVPADWNMGQVLGEQLQKIAQQVGPEEHAICALSGGVDSTVAATLVYKVLGDRLHCVFVDNGLLRFNEQQRVMDTFKNHLHLPVTCVDHSKVMLDRLKGLTDPEAKRKAIGAEFIEVFKNYASELEQKLGKKPKFLVQGTLYPDVIESCPPPGSGQKHSHTIKSHHNVGGLPKDLQFTLIEPFRELFKDEVRAVGRLLGVPEQFIKRHPFPGPGLAVRVLGDVTEDSKLDTLREVDEIFVNTIREYGLYDEIWQAFAVFLPVKAVGVQGDQRTHSHVVALRAVTSADGMTADWYSFTPAFLRDVSNRICNKVRSVNRVVYDITSKPPGTIEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3U6</accession>
    <name>A0ABQ5S3U6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007841</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037">
        <property type="entry name" value="FORMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037:SF24">
        <property type="entry name" value="POLYCOMB PROTEIN PCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="4">
        <location>
            <begin position="61"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="4">
        <location>
            <begin position="462"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="229"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="662" mass="70636" checksum="F10757444C390A97" modified="2025-10-08" version="1" fragment="single">IHSSVNAGMTRGSSLSPLWLGSVVFCMAILSWMPHALSQEEDGSFFDAGLVEPQAGTERKFPYCSCYRPPANPSPYSFLPTITVPAKGQYCFMLRVNTTGCNTGRCCDVGLKKIEFDVYGKCNVKSASVKATVAGRFTGTGPNIYIYKMDDGTLKYVLRLTNLNLGLWSDNTQICLNLTAGTNDAGCDTLEELCVPPTASSAPGTCLAAFFNDNESTGSCCPSTIVTPSKPPPPPSPRPPSPLPTLPETPSTPPQSPPPLHPASPPPPPPPPPPPPPSPPPPPSPSPPSPPLPPPSPSPPSAPPPSLPLPPPSIPVPFDGKCSQCIKIDLILAIPFPPFQLSNRTCRRVLGHLESNLNQIAAILNVSMTSKNFSTGAQYCTNSTIVVCGEFSNKNAVKDNEALQGSFRIVAEDVVIQLINALCPASSALTIKATFAAETTDEEICFEMPPASRDCRPLPRPFPTCKCDLNKAITPYYTLPDINTAPGRRPGSKLYCFAIDMVPEQYYNRTSGCRSVERVEKVEIYADELRRRNVTGVRIKPNGGPARWISPSWGPSGSDTFKVTPLQWDFTSAVGGSFCIELTIPLKAFCLEQSAWHSLRNQGRNTGMVATAGHQTAALTAGSRAEHCTAVVNTGSCRTGLVTGAELRAAGLRAAGDSCETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4Y0</accession>
    <name>A0ABQ5S4Y0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_007770</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="34"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="325"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="943" mass="102018" checksum="AD31CD40117CD334" modified="2025-10-08" version="1">MMAAAGWQPTQEGVLQLVNLLTLYQQPGTNQTKIFQQLEAYRAYPDFNNYLAFIFAAGEQLPVEVRQSAGLLLKNNLSKQFKDLPAGFKSYIKGALLPLLGHANRQLRHTSGTIASVIVGLGGLDEWPDLAAALPRCLQAEDPNVLDGAQDTLYKVLEDHPSQMEVVLAGSDGASASSLVVPPLLQLLRSAVDDVRCMAVACLNLMAPHMLKGLQENVEGYLHGLFALANDSSNRVRKEVVSGLVASTTTLPERLVPFMRQLVEYMLASNEHSDPAVALAAAEFWTAYLELQLDPGLLRPYLARLIPILLKNMVFDEYDDEVADAEAAEGAATQEEDRDQDVKPFMPRTREHGAPTAGADEAGNGGDGGEAVADDDEEALNAWNLRKCSAEALDMLSNNFGDDLLPVLLPIVQQRLQDSNWRSRESAILALGAVAHGCHAGLQPFLEGMVHMLLPALRDARPMVRVITCWTLGRYSHWLFQGVVDCRGGGRPLLDEVMIGILRSMGDSNKFVQQAAVSSLAVIVETAGEGHDSPDTLLEPYVKAMLEALAAALTCYTRRGIVVTFDALSCTVRVLGSRMADPTLAGIVLPPLLRKFTSAPPTDKDLLATMECLANVTPHTGRAVELYAKPMYDKATALAAVYMHTGQQQHQQAGEASALTTAIAGGVSADGGNVEYDPNYIVLALDVISGLAQALQASIESLVAASPLVQMLMLCCSDPSPDIRQSAFALIGDLASACVAHLLPVLDPLVSCALTMLELPRITDINLAAANNACWSLGEVVIKVDAERIAVHAEAIAHRTTSILSYKGPGRLPHSILENCSITLGRTAWRCAEQLAPHLGHFALQWCGQLRNIRDGTEKEHAFLGLCRLVRMNPEGALQAFPQLCSAFASWRRIGCEGLRNEMAQILQLYKANLAVSGRWEQIYADVPDSVRGKLSQMFGHIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S571</accession>
    <name>A0ABQ5S571_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046029280" evidence="2">
        <location>
            <begin position="20"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="357" mass="37322" checksum="FAD0B325E5207996" modified="2025-10-08" version="1">MWNIFFLLLCLWHARCGLSAKPAPLARPPMPSPPSVLPAPVPENTLWSTFARDCKDLNGQSKRLGTARNVIGSPGRAAMQRVARSICSDTPNLTWVSDSPRMCYLDAFFGTESLPESSGLVRVADAANIAILVLNRGTVRPIIEAVELILIPPHNKTTDDLPGVNPAPKPPITPIYPPKPIPPTSPMSPSQAVADPTTSIDTSSSQGQGPPAAVTWGMGFRIGAGTPLSSLPPSVSNALAAAGVDTSDSRTPVLMPVFRSNTATTAQLLVNCPGWNTWPMALHASAPSVPWFPEALVLGVRVTVRGAGSALQSHIIMPGLAAVGLGLKVSQLYPSPALSPPPALSPPPAKASRPTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5Y8</accession>
    <name>A0ABQ5S5Y8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008506</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF24">
        <property type="entry name" value="DEXH-BOX ATP-DEPENDENT RNA HELICASE DEXH11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="469"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="752"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="398"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="669"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1137"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="675"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1137"/>
            <end position="1154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1547" mass="162217" checksum="CC275EC5B254419B" modified="2025-10-08" version="1">MAVVTLMDCLPQMELLYGSGGDAALVPARGELLPPLVQAPPFSALGNGAFGGASASDAVLAAVWSAYLEHDTEEAMSAVMAETAHPLITLFPRARAWPEVSLPSAIVEPTPPPPGGCEQDVGWQVVEVAAAAAAPDGSGGGGGGVTAAGRIDSLALQRRLAARSGGTDSNWRGLSVSLPYMPGGGVALPGPSGPAAAEAASGLWLAQLELGLERPMGLPRVAAPGLGRGLFGGWAADSQVVSELASDARKSDAEEDWVAFTGHHRAAATPAAAAPLNAGVYPPMPLTTPAGEQIKPLAEVLQGGTASVTAVNGSGSRAIAGTFSDLWLEPELAAMAEAEGGTEETEEVSAAGAVRRRGGVLAALEDEAARGKGQAADSAEGDGKLAAVLAAATGRDGSGADVAAATRHDQYGGPGTSPSPRKLAPGATTEHPFAARNSIRNVEEEFNKLRPRMALHYPFELDTFQKEALLHLEAGRSVFVAAHTSAGKTVVAEYAFALATQHCTRAVYTSPIKTISNQKFRDFSSKFEVGLLTGDVQVRPTAPCLIMTTEILRSMLYKGADIIRDVEVVVFDEVHYVNDVDRGVVWEEVIIMLPPHITLVLLSATVPNVLDFADWVGRTKRKVIHVTGTTKRPVPLEHSLYYGGEIYSICSREVFNPDGLRRAQAAFKARNGPPERGGGGGGGGGGGGGAGGGGGFGGGGGGGRGGGRGGGGGGYRGGPPSGADAARRQIASANRSGGANSGPGGFRSERQQLLELVGVLGKKAMLPVAVFCFSKKRCDSCADALSSLDLSTGSEKAAVHAFVDRCLARLKEGDRKLPQILRLRDMMRRGIALHHAGLLPIMKEVVEMLFCQGYIKVLFCTETFAMGVNAPTRTVVFHSLRKHDGKNFRYLLPGEYTQMAGRAGRRGLDAVGHVLLVCWGDRELYGESELRTMLTGRGVKLESQFRLTYGMILNLLRVEDLKVEDMLKRSFAEFHAQRAAPAGAAELATLEAELAAAAAAPWPATALGCPREEVEEYADFCEQLEAIDAQLQDALIANKSFQQALVPGRLVLYGNPINGLTEPAVVLGDAPAPPSPPPSSPLTLNAAKPASIIVGTATTSSTGSTTVSGLSIAAPGATSAGASSDRRLYLLVLHRPGSMDEQHERQEQERRAREGPGGVGVGGMTSLHSRKQQELDDVFSGMVKLGGSKGGGGGPPRPLPVPHYGCEAGIHYCVVADWTRSILGVCRGRIRLDGADDILSCGGNWTANAVRRALAAALRGIARTVDEATGSDSGGELPLLDPRVDFKIADVASVPALQARAALQSRRAALRPHRDSGLVDAFQLVRTRRRLERRSAELRHQLSDASLQQLPEFEQRVGVLQQLGYLEEDRSVTLKGRVCCEIQSTQDELVATEAVFSGLLGALAPEEAVALLSAMVFQEKSEVEPRLPPALAAAREALVALTASTAAVQRSAGLDISPDQHCAEVLHCGLMEVVYEWARGTPFSAITELTDVMEGSIVRAMVRLDGACRELQDAARVMGNTALFQLMQAASAAIKRDVIFAASLYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S691</accession>
    <name>A0ABQ5S691_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008797</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14133">
        <property type="entry name" value="PKc_DYRK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF124">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="330"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="636" mass="70911" checksum="B2709D9BBFB72913" modified="2025-10-08" version="1">MAPLLHRQQPVSGRGTLKRTRFLVKYTHLVVGRHGLLTGSASLVPRSPILSSVRGFLPSAQSTYSGVTSSIRQASLEEDEYVGRARELQYPSEGPYVDEYDDASDMGFWKVDIEDQAAFICNELDLTDEQALYLLHALGLLSEGATLEDFRDARQQRGGGPNDMDDPALRDALEMWQDDDGDDETHARRSGGESTPGLGLAAEDYGEEAAMYHLESDQAGQYEGYRTYHDESRLGIQDYGHSAHAAEDHNDAAPIKTGSFHVSIRPNLTESKPSGLKVAPGVPAMAYDQTSFDRDFEVIQLRVIGRRASTAAAMGDEIELSPGKLIAGRYQVLEQVGSAAFSRAVQAYDTLERRLVCLKVVKNDKECMDACLEEVRLLQMINARDPHDQHHIARLYDYFYHMGHLFLVVELLGGNLFDTQCYDPSYFTAGRMQSIAKQVLQALSFLHSNHVIHADVKPENVLVKNYSKCSVKLIDLGCSIYNSETNMSHYVQSRSYRAPEVMLGLPYDGRIDIWSLGCVMAELATGSVLFPNVSEAAMMARMVGMLGQFPPYMLKQGQYAGAFFTRSGHVYERNGETGLFELLAPKRTTLQARVPAADRGMLHFIRHLLVPDPSKRPTAAQALKHPWLQYRYRSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6K4</accession>
    <name>A0ABQ5S6K4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_009114</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="60"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="133"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="150"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="574"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="747"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="780"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="588"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="780"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI65552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="867" mass="90779" checksum="C71A7F59D566DC98" modified="2025-10-08" version="1">MGAGASSQALTRYKWTQDHTLRAPWTFIFKEKVQCACEGSKWCSRENPARQKTANAIPGLHSDWVSDKVLAMARPWQSHVEQFKLLEAFQHENIGMILNLQEVGEHANCGPGNLPHCGFTYDPETFMAAGIGYYNFSWRDMGVPSLDRMMDIVQVMDFVTRVEGRKIAVHCHAGLGRTGVAIACFFVFSGLYDSPEAAIAAVRKSRLGAVQTSSQVAFVTIFAQYLQHLRCVFPVAKHSTPPRPLPHSNSAANITDADKMAKTASGGGGKGSLETKSAPPGTLLTDGLVESRETGTAVVGVYSSGLTVTVPLPSSTSAAPPGSGGGDTLTGADLGLSRHLPWNDAFQIAPEHAADFQPRPPRSYPEALARQARLLHGFERRAFLRLHKLVKEAVFALIAAAHAFQPTTRSSTRALLSLPAILEDEQAGVIQRAKSGAAAMAAALAAAGNTGGAGGAPLAPPLPPSFAHLLSYLIAHHGPAHVDSDALTPLMAHKAYAAAVGPQVESDAVLAAALHHRHELNSGRYEGLQRAPLLVVIMLLEAFFANFSAGEEPASLTAGCLSVISQSYRDLFNGQMPAPPSRPSTADGSGNANVNENGGAGNAAATCNGSAAAAGGVPIPAISLEYARQAYEILEPLETPDSELLLVFAAMLRVVARGSGPGSEDAVVEVGKWATGLLLGKLSRVPEAEEAVLSFLWYLAGRDEAYAMLVLLKRQRIAGLPRRYKPSSGAPDIAAMLQATAASLAVAASPPSQSPPPPPAVEVSTESISTATATPATLVAPMSAGGSSTTDGGSALPSPGANGTGGIAAAAAATVVTMQALQMSQLLSQDLQQLYQVSFREALGAISTVVYPPPDAMARLNVHDVWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6Q0</accession>
    <name>A0ABQ5S6Q0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009149</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045710933" description="Guanylate cyclase domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="1936"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="3">
        <location>
            <begin position="632"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1663"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1870"/>
            <end position="1893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="567"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1666"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1870"/>
            <end position="1890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1936" mass="206288" checksum="3D1E8C6F16D0E2A8" modified="2025-10-08" version="1">MIFHKITAQVFLVLLTPYLIHGVRAVEIAELEQCLETIQNNTSWDDICQSPDNFATSLEQGVRKHFSTCLNENLARTLRITVPNASLLPAIRRHALEFQAKYNVSVKLNVLEVLNIATEVRGEAQSQLQDFSDGWIVDPSVTGDLAAANALANLTSYVFADQDLDWSDVSKFFRMVSAYYNKRIIGLPLDGDLLLMYYRRDLFERYNLSVPATWEEFAELAGKMNGTDTDGDGVGDLWGACIDNTQPTCKSLWILTAILAPYVQYLGTKQGVFIDPATMKPLMNNPAMIRAVQLYRSLFASGPRPAECRAANPFFSNGSCLMTINFGDQFKVSAQVTANNSNRQFNGSMLGISRLPGSLEVLNRTTDTLVTCTHDTCPYAQLVRLANGTKAWVNFAPFSAFGGWTGLVNARATQINQLWAFKFFSYISQPNNNIGDVLDPTSGIDPYRTSQLQPSPENLARWAAKGYDINATLEYLLTTRDALDYENMVLDVRLRSMADVTRFVLATVADNTTWGKQAIDQILIWGQNLMEFELNYSKTNLRDLQIQYWQLIGYSPSDDRGPRPNQKPESNSTNSNNVSHGSGDIVEMAVLIPVGTVLMAVGACLLVWRRVSGHRSLLYGSVKAPMAGQQTTLLITDIQDSTSLWEVLPDVIMDAALKVHHTVVRQALAKHVGYESATEGDSFILAFHSAEDAVACAMRIQMDLLTAKWPPDLLRCTDCLSAAEVWVGPARGALQQHCALLDMLGGSIRCETRDRCWTFHRTMVGDFCNAVARAPWRLLDGCRGGAPMWRSSRTLLKRRATACNMQPQQQLTQQPVQSLKAPQAIAATNGVAINIKSPDVAMAAATTAMPGTATEKDDPDAAGRLKHQISEEEQRQLSAHQFWRLSTTSGMLGEDDTTVVATSYWSTLQEGMLSRGTSSQYRDEAQGASPRWLGALANIFGLIFRSRGQPMHHNQQYQYLIPPVFVSTGDKKSEQQNGKSSLNGGAPCTTTAGVLNAAATGTGTTGPGATAGPGSGRLFKEHIRSIWFVVDLGRCTFRNVDTDTIIPPSSGTGGELQSSRSSKDGTIAARANIETTLLAPTSASVPVQPTRGNIVGGADGHKNQVLKPTASSGDTQLLQHGATEVEMSEIAITGGSKNKLLLPHQEQPLQPPSQRIRISQPQQINLVDDSAVANPASRQHAVRQLLAFRGLRVRMGIHSGVPDSEHVLYNQAEARYQYSGVGMVLARAVQGAAAGGQVLLSDSTFALVRDGRRAKRLRDAVLIHAGEHVLDTKLPAAQQIYQALPRQLLCRLALQPPLKVLRTVAAGALDAPVGRVAVAFLTVVGAATLQSWNAEIMQQALGVFQAVFWTAAAANATRGAYLVEFTSEGLLLAAFASTADALATCMAVQSDLLVAEWPPELLEHDMCEEVAIATPQPGGTIVREVLFRGLRVKAGVDCGLARASLNGATGRIAYRGRVMNRAARINARGSSGQVLCSRSAWQAASEAEEVGPAARGLAALSLGHVMLKGICEPMEILEVRPLVTAAAPSPGQSTLMGRTMLASPSCGSLAPLASAASMSTVVAGASSLGPTPSVTSRMTLPVVGAPTTQHSTLPDGMVSALGRTNSIRGFVGKMSVHDDANVSIISVDVPTAVSDVSANTSAAVVPVPVAVAVPAAAVMQGVDPGSGQTSTSNPFKTRAATTPSISGGAEIPIGPYRTCPTPIGMVPMTSVSLCNLHQGESRLHQGDNGGMMATAAIATASIGTATATAGASSRNAGDLARLNSDASGAVLLQHSGSSFIGSRLPQALSMTAQLQTAQGLRAAAPESKWLLRASSRRYSGIEQPLPPSLLNAELLLPSSTGATGTLPPPPTVQVFRVDTTTGIAALELPLPSPAPPPPPGALNPPTPAPAPAIMLTHDTTTSSAAVPGCTALPAPHSMMMGATGDVPALASQMGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6U4</accession>
    <name>A0ABQ5S6U4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-ketoacyl-CoA synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012392">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013601">
        <property type="entry name" value="FAE1_typ3_polyketide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31561">
        <property type="entry name" value="3-KETOACYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08392">
        <property type="entry name" value="FAE1_CUT1_RppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036417">
        <property type="entry name" value="3-ktacl-CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAE" evidence="3">
        <location>
            <begin position="8"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="2">
        <location>
            <begin position="405"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="52519" checksum="8C12CDBCE6293F83" modified="2025-10-08" version="1" fragment="single">MLSLFPPRVYLYDLNIYKPPVSFKLDQRAVIRTWHDSQFYTPELLDFQTRVFARSGLSPTETHLPPTLNPAYVGSDVRTDLEAAAKECRMAVCGAVEGLFKKTGLDPADIDILVTTCSIFCPTPSMASMVVNAFGLRRDVQSYHLGGMGCANGVVAVNMVADLLAAHPNSNALFVTTETLAPAFYFGTERHRLVTNLLFRMGAAAMLITNKPSLVRPAGRGRRGAKYQLLRRVRVHTGQSDDAYTAIHFSPDEEGKNGIYLGKNIVKEASRALSMAMTQIAPYVLTWQQLAEAGIDALRRRRAAAADRYRNRQRVDASSVDKSGVGNGDGSSSSGGGTFGGSSRSGKACVTAAAAGHDDTAAESSADTAVTASTPADKAPVAGASRSSTTPSYKPNFQNSTVSHFLLHAGGAKVLDGLGEALQLDDTKLAPSRNVLHDYGNISSSTTWYTLACIETTQGVRRGERILQVGVGSGIKCGVNVWKALRNVHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S849</accession>
    <name>A0ABQ5S849_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Membrane bound O-acyl transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009807</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051085">
        <property type="entry name" value="MB_O-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285:SF18">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE RASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="549"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="586"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="204"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="631" mass="69216" checksum="51EEDBE3614EDC3C" modified="2025-10-08" version="1">MERRDRFLLAERLIYLALVAGLQLNVLREGYHFAKKVQSIINLPGLRDEGPYFGRNDLSDYQWRSFRDSAPLLAAAMVTFIALSQLARVFPRIRQAVMVALSMVFLCAVHGACAAYVIALTGASYLLSRRLAGRPYGLLGVWVYGCATLLLARLSEGLPFAWISPRLAPLDHHRGLLRWHIHYNLMMLRFISFASDLHAARRGKRTKRSSGDSSGPLSAAGTRAGVGAGAGSELGARCSPEEEDQRTTSGETTEERYEPHLASRGGYGAAAAGGGAAAGDRGGSSFVNDELRALTDAPLPLPYYSLLPFLEYVLYPPLYIAGPIITFNSFAAQRMLKRQSLLGPRQVLAYALRAALAWGCLEGLTHALPYNSIAKHRVLDRLAAAATAAMSRSGGSDVGRLPPWAPQPVHYAITGYWVLIFMWLKFTVIWRFFRLVSLLDGVVPPENMTRCVCNNYDIEGFWRSWHASYNRWLVRYMYVPMGGSRWRAANVWVIFTFVALWHDLEWRLLGWAWLMAAAVAPEMVAKALGRSAAVRRWYGTATFRLASSLAAAANILVLMVANLVGFVVGLDGIVPLAQQVLLKQPAFLGVVFLALFAAVQLMFWKREWEGSAAGTRARAAVGMGAGGGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8A2</accession>
    <name>A0ABQ5S8A2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="291" mass="32746" checksum="C62FDD455AE2B4C1" modified="2025-10-08" version="1" fragment="single">MAGMAQGNDRRLGFSHATPDDFRRRYDGITLQEPSNLLPCFKYKKASSVIWDLAASSFDCVASTFMFGNYERGLQIAIAPIFATVAAVLDRELKCECYLKSVKAKGAADYVFQNRIGRQKNNVINTLVIELKDDLDKKYENNRAQLLFELIAAWEWNFKKKKVNKTDAWRIPVWGILLDRYHAEVFKLEKIDQYTKVPSIWCSRRLSLFKKIENPARVVPSDDLPLVFHYILQAVNDKCVAWSEEIWQDKASEIRNDARAADGSSGTQGGSGSGPMPRGRKPTLGAAAGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8B9</accession>
    <name>A0ABQ5S8B9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="172" mass="19372" checksum="9C739C7D44E49F43" modified="2025-10-08" version="1">MDFVVVLPKTEGGYDAVFVMVECLTKMVHLAPCTSSCTAEQTARLFFDNVVRLHGVPKNVILDRGGQFTSKFWEALCKLVGMRVNLSTAYHPQTDGQTEHTNRTLGDMLRNYAGRTPLVWDTFLTAAEFTLNNAVNRSTGKIPFFLNYGFHPTLPMWRELEVDVILQGEVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8N3</accession>
    <name>A0ABQ5S8N3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000030">
        <property type="protein sequence ID" value="GLI66303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21087">
        <property type="entry name" value="SHIKIMATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21087:SF16">
        <property type="entry name" value="SHIKIMATE KINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="329" mass="35951" checksum="AF588FF13D82DA71" modified="2025-10-08" version="1">MRSMDFRPQTGCLPFKDPMLSPVTARVVTSFSCACILVRPVVRTSSAAREPHNEPAPSTSTVADVDKEVVAEKTRDGMHRFAASTRKVGATRLVVDSLKRVSLTFLGDDHTLNYAVAKVVGSRLGWFAVDVPKVICGMRKAVSLDQLTQEERAIAEVEVLRGLRNQFRIIATPLPGGAITHEAVWADLWGSVIVWIDEEDKQRPKGRTPERSLYEDRAEVVVRTKVQKGFAVKGGNSLENRAKAVAELLLPRLSDYLAEFPELADRKRQYVELGCRGDWPHLQSPSCSSVVQGLAADRKPLVGFESSGNAVADGNAMELASEGTPAICV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8U4</accession>
    <name>A0ABQ5S8U4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010112</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028:SF7">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="556"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="3">
        <location>
            <begin position="102"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="581" mass="62916" checksum="3EC765361143FB36" modified="2025-10-08" version="1">MLVQTQQRLLNCRHHRHANDRIIPGVVTNCISSLGLPSIAIGRPAAGAWCGLSIGGQFYIKLHCDPFLTRRRIITETFADPVMDAPDVEEEEGRRWDPTTLTAIVVGAGPTGALAAIYLAMRGYRVEVYDKGLRPLAGDAASIPLVLTSRGAIAFEELGLDHQRRVGPNATALRGVWDVVGHRLQPLDSTSAFRRTFVTDRSGLAGDIIRAAEDRYPDTVRFHFNTELTRAELKNRVAGVRLSAAASASATGVAVDVDEQERELSYDLLIGADGTESTVRRILKAKVKDFGVIRPVEETAAYKAVSRLPRPRAEPLPGFYTHRPQEYVYDWSTPGKPSLRLFIDADGSIGGTVTGLQRWDDAGAVATELLAAYPGCLPLEWAEAVGKQVSSPSSGEPRHAAILQCSQFYGPRTVLLGDAAHAVTSASGPVSLGQGPSAAVESVRTLALVLRGAQDDLDKVPEVYSNVRGDAVMAMQMLEFMELTRDPARMKGLKFASLWFACSAYVTQGFRWLAETVGGLLSAILPKTFLSAPQVLARLEDRRIGYSEAIKMLQGYASPVVALFLGGIFLGLFMAFNRALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8Z3</accession>
    <name>A0ABQ5S8Z3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L13a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_009707</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="22596" checksum="2DAE68E805EE8770" modified="2025-10-08" version="1" fragment="single">GSLEPSLSLPLAALVARTMPKTVVIDGRAHMLGRLASVVAKQILSGQQIVVVRAEDITVSGGLVRQKMKYDRFLRKRMNTNPTRGPFHFRAPSRIFWRTVRGMIPHKTPRGAAALERLKVFEGVPHPYDKVKRMVVPDALKVLRLQHGHRYCKLGELSAAVGWKHQAAVAELEEKRKAKAKAFYVAKKKLIALRAKAAAQVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9T4</accession>
    <name>A0ABQ5S9T4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF8">
        <property type="entry name" value="SD21168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="439" mass="48165" checksum="916D1B317631607F" modified="2025-10-08" version="1" fragment="single">PSVSELDSLGLSDSTFILFTSDNGYHEGVHRMGSGKTTAFEEDLRVPMMMAGPGLAAGSKVVTTTQHIDIAPTVLALADLALSLRQLDGVPLPLAAVSDGTLPPSYVYGRPDQGPLRNHLMAEFWGEWVEESIHSHPPYQNLTWKAVRVLRSDQEEAYKYVLHCSGEFELYDLVRDPYERINLLLPLGGASVSQSGSWWSSARSAVSGAVQASFRDKINNSSTAISEVAAAARAWARTVWDRSTTRGSASAANDHRLRNTESRQEVMAVNWRADGNYVNWLLRIRSRLDAMLAVLAVCSGRSCSHPFQVLHTDGSVWDLWDAMDEQYDDMYDAIPAFSFGRCLPYQDLENEWSSFRGAVVPDCAENGSAWSAPRRVFFADVGPHAGSREAFRHGNSDAGDVFVIVESELESAAVPLPAKYQMVTDVYGSNAARDAILLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9V4</accession>
    <name>A0ABQ5S9V4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009980</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="3">
        <location>
            <begin position="194"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="651"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="666" mass="73427" checksum="9467E9CADE02F87C" modified="2025-10-08" version="1" fragment="single">MYFRAQLKLVIGVTIWLTTVEFNCFRNWSIMLPLRRDNLPGRLGGHKVIRVWRSGGVCITAMLNYFAYFLLVILQLATLPPNTLGRRILAGGAQGVLQGTITSLELYGSRPTYYLDLYGTSGAARIFHLEFCEAVSASSILLGILVNVPYDNIENGVVHSCSVPTEPDQESQPSRPQRLLLGEAITSPLEPRILVYIASICGYNKPPAASKEEVMKLLVSGDSTNRSLVEYFRTCSYGQVSLLESNIKILTPPAEIPCTGTLPPGQPFATGPDFTTRSCNELDNLPKWHYWLDAWAATIGVDAAEYHQRILILPREFTTRIPGCNGFSGLATSGRWQYWSRTGANSWGTSLIWLTGENLGWLEVWFHEIGHTYGMAHASIPGGCGIGDQCDHTCPMGATGGQGIRCLNAPHNWQVGWGGPILQLSDANLPYGKPSAPIRIPLQTSSINSSVVVTGAGMPDNQRLFLSVRMNVYPYDLAWKNLDDNTAFVLVHSYNGTDRSFYHPSYHFGETRLGSIWRDTVTGSNLSVRFDYWNTDTGASVRFCRRTSDTELNCNDGLDEDCDFLMDSEDPDCGKSPGLGVGATVRRAPQLFLQPFISTFRQSPPPLTRSPHPSWPIPAPRKPKVPKSPPPPPPPPPSPRRSSFNPQLSSPRPPPPRPPPSIPPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAB5</accession>
    <name>A0ABQ5SAB5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006076</name>
        <name evidence="4" type="ORF">VaNZ11_010201</name>
        <name evidence="5" type="ORF">VaNZ11_010282</name>
        <name evidence="6" type="ORF">VaNZ11_010287</name>
        <name evidence="7" type="ORF">VaNZ11_010793</name>
        <name evidence="8" type="ORF">VaNZ11_012010</name>
    </gene>
    <organism evidence="4 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="4 9" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000042">
        <property type="protein sequence ID" value="GLI66838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045029980" description="Secreted protein" evidence="2">
        <location>
            <begin position="34"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13330" checksum="75934609544F3F8E" modified="2025-10-08" version="1">MCMHCNLSSLTRWHLGRLAWLFMALTTTGPAPAVCSLVSQGGSHYFGGRYGPRYDDDDDVIPRSGSDRRSGRFGALLEPLKRQGGLADPHEHFEETEGDTSNKAYRKPRIGIRPMAHDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAC6</accession>
    <name>A0ABQ5SAC6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Signal recognition particle subunit SRP68</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_010384</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SRP68 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15481">
        <property type="entry name" value="SRP68-RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.40">
        <property type="entry name" value="Signal recognition particle, SRP68 subunit, RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026258">
        <property type="entry name" value="SRP68"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034652">
        <property type="entry name" value="SRP68-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038253">
        <property type="entry name" value="SRP68_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 68 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16969">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038995">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI66515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="678" mass="73115" checksum="3FDB06F49AFE54DB" modified="2025-10-08" version="1">MAEDQPETRVVDLTGAAPDESTASSQISPLPLTLLSIIKTAQAQNGLRHGDYMRYRKHCASRLQSLYKVLRMQHGRTKYQKRKMDIQHITDARHLHVPLFSAERAWAYAMELKKEAEVQGGGGAIDRRKRHHLIGRLSKAVLWASELARFAAARSDTRGSLEAEAYCSWMSGTLLLERETDWELALAKFTRAKKLWQELAKACPDLEKQSLCLAQVEEMEPNIRYCNYRITRAGGAVPADPAELLAMAEAGGDVPGLDVLQSKLAALAQEAQMAQAASTSYVSWMGVNYPVRHERVKLALHQAVEFGTQAEQAMNTDESDALDSRLALYDKAINALAEAKSHVRNAVKGLAGPDAEVHFKELEALEAAVEGLRLQRTVERSEVHLDHTRTRFTAGLQRLAMGKKIKDKERHARPEEVVRVCDLLSRHLEDLADLGARLGGRAGEALHDAAAARAAAVRAARTYHIAHTLLAAAVAAAAGGDAKEATSGKALEAWALFNRSQERATAAAEQLREINVGDAAAGVLLGGRDAVLADLAELVKQAAAYKAVAQAELTAAELRAKEELLQSGVSGLTLEQQEQAGTTSSGATYLLDALGKWESFAGAPAGAGAGAGAGAGGGASATKAAPRLYKAPYDFAAIPVRPIMLDTASNCLSYPGLEHRLRKAEKKSVLSSLFGWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAJ0</accession>
    <name>A0ABQ5SAJ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010901</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000046">
        <property type="protein sequence ID" value="GLI66895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="18597" checksum="09A64951875133B4" modified="2025-10-08" version="1" fragment="single">VTFTNHFVTTPICCPSRVSLQRGQLSPNTNFADVLGPHVFLGGRVASKPHCDVRPIVITVSQEVLQNSVRWIWIASGSPPGCRKLGTPRYTQPPSKRVSLIYLRPDTVGGAEWSGTATTAQNDPGVSGKPAWLRARDPLTAANVTFLEEIHRVPLRCMRAVDERWSWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAK3</accession>
    <name>A0ABQ5SAK3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002136</name>
        <name evidence="3" type="ORF">VaNZ11_010939</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000049">
        <property type="protein sequence ID" value="GLI66910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="255" mass="26812" checksum="1DF820DED940BB8F" modified="2025-10-08" version="1">MQASNLANAVSALAANTFQFVGRIAANASQANRWEWLSRLTQPPNAAVAGGIDLPNQPSPIPRQGGTASPEAPTSPIYAPSPFYVHGPAVVSNHLTPGAVDIPGMAPAAAATTCAAVAGTSARPPGPPHTGHEDEDMACDDPDEETPAPPTKKPRAASVRPGITGHEDQPRQVKGAGKSKRGGGQQGRHLAPKGGHVQKKGPKQNLAEYLTTFKCTRREFHFRKQHGMCLRCGSDQHRVDSCPMLGAEKNKGKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAM7</accession>
    <name>A0ABQ5SAM7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010380</name>
        <name evidence="3" type="ORF">VaNZ11_015057</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="2 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000034">
        <property type="protein sequence ID" value="GLI66548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="16033" checksum="17B8960250E81347" modified="2025-10-08" version="1" fragment="single">QVSDLISQLEKLRSGAAMMEARKGGLSGASTDLQGSSITAKFAIIPAASCPAMGATSPPSAVEVADAGAASIFINKAAQQPQELYQPLELTQPPKPHPKTPERRLPPEARNWGLQDCRSWAGEQHWSSWAEVQAFTKTLEPTLAEAIREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB51</accession>
    <name>A0ABQ5SB51_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011072</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045042511" description="Guanylate cyclase domain-containing protein" evidence="3">
        <location>
            <begin position="34"/>
            <end position="3169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="760"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="909"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="4">
        <location>
            <begin position="811"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="4">
        <location>
            <begin position="3061"/>
            <end position="3098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="943"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1303"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1425"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1516"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1642"/>
            <end position="1663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1680"/>
            <end position="1746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1770"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1822"/>
            <end position="1856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1968"/>
            <end position="1992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2008"/>
            <end position="2060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2269"/>
            <end position="2288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2413"/>
            <end position="2551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2681"/>
            <end position="2706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2939"/>
            <end position="2967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="948"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1331"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1425"/>
            <end position="1436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1456"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1518"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1680"/>
            <end position="1705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1798"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2016"/>
            <end position="2025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2045"/>
            <end position="2056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2413"/>
            <end position="2424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2465"/>
            <end position="2477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2478"/>
            <end position="2511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2534"/>
            <end position="2545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2696"/>
            <end position="2706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3169" mass="326715" checksum="C2709E51A58F90F2" modified="2025-10-08" version="1">MLRSQLRCFPQQSAQPGLAFLLLAVIFLDPCYSAQTGSGNATCCTGLLCGLENNSSKVPEITILSGLRPNAPHLQTLAREQTHRALAAVRDPRLSRVQLGGDAGAGDVLQQEGPGISGTWYSPAGDVDLELLWDVRYLNDTSAQMLIAAAAADPVRAAGRAVTPVAAFRSPWNARLCGALEYMALPLLRAHSSGNTSWLEQLEAAAAAGEHPLSNEMLVPRPGKPGGPAAVPLLLLYRRDWWRALLAKAGGGSSDPSAEEEVAVLPPTWPLLVQLVSELLNRDLDGDGRQDHVLCVDLMPGCKGWALLSAIFASLVQTNGTEQGVWFNLTDLMPALGGPALPTALRLYGGLAASNAAPFTPGGPSGSRSSVPVAPSELLAGGGAVDAATGAPLCGAINPLFAAGRCLFTIDWAAAALRLTKEAAPGIAGLVGAALLPGSAAVAVAPTIPSPSFASTLASAPTPAPSPASSTPSSYFQKLSPAPSRVVYEGGNGITAAIQPCSATTCPYGDVVSEVASTALAAVMNKTATARAVPRYVNRAALVGDATNIWLLGLGQSVDSFIQTDDFIRLVGFQLELKYTALLHPLQGQQHSAAAATYVGESGQAIPTMRVVYPGMPVDLESSDARAVLKAVNASVTHPNAAMDILLPYSSSYRSIIDDLATAAAELLLTTSDSESKGAAAAGSAAAGNNATSALSSQLEALIATAAMRLVNVRNAFPYPVILQHLYLHSIGASSPNGPSEPPGGGGGGRGGGRIVPWRLAVAVALSIAGFLGLAVAAACLVASLRRRWRAKRRPASSCRTKPPGAGPATTLALTDVQSSTMLWEVLSAELMDACMSIHHGIMRKAIEYHSGYEVFTEGDAFAVAFHGPGDALGFALDVQAAMLAADWPPELLDHPEACEVWARRNSHLNWTASISNAAAAATAAAAGASGVGSPRRITGAGIPSVAGSGGGGGGSGSNAGGSNVGGTGGGLENSLIGLSRLTKGSKRLRALLGGGSSKGEGSSSAAGGGNAAFLTSGDSFWTPGSAAVLRLLPSSNTVAAAAAATAARQNSPQSQRPFSSFLSRLDATTMSLGLVGNPSTVTAARAAAEVEMAAALAQYGSMAQAPWNNGGPRPIMSLTKYDWFRNTPTNGSSAVDWRPGDNANNPALRMGRVFRRQSAEGIGPWDSPVIPRGLASGPVVPRAATSRPLTFANLWAAGVSAAQPGLPGQSESPTPNGHVQSFAYGTWGGSNTIGPMPPPQMGLWNAQMRVPPPVWLPPPPTILRVGGELLERSVSLSPPPHLPHPLRIPMAYLAASRSAGAGGCGAAPRNAPPMRPMHSLKPPKPQDDNGTAVATATPTPTLGTTTSSARRPGRRCPPVKCASIGPSSLIRPFSEVPSLPRTSGGGNFKESLIRTEAPSPLLPMTVELPPACATGASVEVARTAPASTSASAQRAATEDLTPNEDLSSSSPFQGAASLATTPAASSHPLQPPSPHLRPAASGQAGESSQPVAVAVATPGQAQIIRRQYSGIIMSPTALQSPQPSPLVSQLPPPASQQQAHHRRGAVRTASRLSLVTMDRNADAAAAQRGCAGRRNRLLSLFHPGQRNTVVSAAAVASQFGPVQYERSPAALTLQRQASFTAISSRLGGASSMALASGRSLSFTARQRPDPDRLWPPTGVTRGSQLHAIISGSSGRAVESSWTQAASSPQSAPVSHYYQHQHQQQGEAQRISLTKKPQETSEGEFGEASRSQERRSFQRGHSGLHQDQQLRCAYEPVLVSDCAATLARMLPQQQSQQQQPIPLRDAAENHPTPGDVSPQPSNAASTSTAVPIARRVSFKLSGLSSATSQEPPRPHLNSDPDPEPDPESDLLIPDRIPGRDWSLHPWLDLQAVRSGGDMYSTRELFGGLPRLAEGHEGDEGTASGTRTFECTVTSPASRPLASREDWHWPQVGPRTDMSTSSLPPSVPPSQPLLSAAAAVLAGLRPDAQRGLVRTSSSSSRRRSTGCSFESSGSAGDLLRALHHEAGSVKAARRRSASAAVSGPSGLPTLAPRVPHVTSTWRPDDPATATTSTSTMAMPPPPNVGELVDATTMKGLIFSAAAVPDPAAQAAVRSIQAARRSGGGGNEHDDQGRGKLIAVDVDVLLEAERVLVRTGGGGGGSSRDIKKNNIISQVRRSCSAVAAGDVAEPHDANVGYAAANGRRHGSAAAANATEHRRDLAAALDRFDDGGGGQLLEWRSVVTWEVDLEEVSEPLGFDLMATCQPALQGSGGGGGGLSQHLPVLSTGVATGREADGSQAGGGGGPASNRSAFFRSWRNMAGGGAPQQPCEFGAAAQPPARRHACNGSSAIASSVSVPAAATAIGGGISAGVVRTNSFLHAGDTIMETSSQDDLRRNSNNAGSGRLLTRLGHAVRKAVLRGTLGDRDRDRERDREFDELNLHPHPHSEGTTATTASGACPARNGRLGSSSWLRRPSRLRLSKDAGLGASAGGRAGDGSGGASFATADGAGEAGTRAGSSTGSRTSSASSGSSRSGKSDPIAGVICCREGGSAEDASGSGGAGVGGGGEPRMDDDVGKEVEEYGAEVSTQSNAPWESTATAEQLAAASNAATAAAAGLVLPHLGPSVIPSMALAVLANDFDDARQYYMRPEYMLANGGAMLRGNVGASTAVAPPPAAPTMPRTAAEDSVFDLLWRDLNAAASGPYSGGNVFSPPPPPSGERPGPSTGWPQAAAATVASALRLLFEQLTHAEAVLSYIMEGGSGVHRENGYGGSSAPELVLRGLRIRIGLHSGPREGEVELSVVDRVSVGRYRGDFLATCKEVSEAAVGGMVILSGAAFRAYHQLRTRARQQQQDALMLLHVGDHIVRSAAHADQDITRASIAMAAASELAAPARELYTAVSPGLLARLAVLPSPVRTAREVVPGCLSAPAGVVAPVFCNVVGVEALIAWEKVLMSRMLRKGSTNTGLPNHSHPHHEHVPHQPLQRQQTGEALSTEGDSFARGVVHIGLDILRKVVSEVAAMHDGYVVASSADGGHWVLVFGSAENAVLWGLGMLEAMLAAAWPEGLLDHEVTEEVWEGSTLLRRGLRLRIGIDCGAAMIRLVPRTGRLDYVGRPLNRAARIAAKAKAATVLVSDAVWVSARASLEPVVVPASLGLVQLKGVKEPLELWALKTRTAGGSGDGGKGVSSGNGKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBI7</accession>
    <name>A0ABQ5SBI7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010798</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000042">
        <property type="protein sequence ID" value="GLI66823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="303" mass="32748" checksum="B809ED2819A7412C" modified="2025-10-08" version="1" fragment="single">MAALGTGMFPNSLCMAFSDFCDWMSGLCKDCCAPTAHPVRIKVAMEQQEPAQYMGVPRDKWNPTSFQAPEVMSIRESSVSPAVFFQGTGSSSMLSNGLSSSSFPSSSSFSVGYADTCRIENPPAMEETTVVFSAMPCEKSAASATALAVEEVECGTCNAALDSDQGGLEASAVMVCAQLGEAAEPKAAFPVTMRKQQNETGILAAPLAEQQAEMEISPVTLDSQPVETEALVTYLSPQDEAEAWEAWVAAENARMQEELEHYDNPNKPIKMPKEVQERMDLVVQQLKDWIVSCGLVMRLWVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBM5</accession>
    <name>A0ABQ5SBM5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FRA10AC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019129">
        <property type="entry name" value="Folate-sensitive_fs_Fra10Ac1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF25">
        <property type="entry name" value="PROTEIN FRA10AC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09725">
        <property type="entry name" value="Fra10Ac1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="36401" checksum="319C79E8FE4B975A" modified="2025-10-08" version="1">MAQLGDLRRAAFGRVQSSQYYQSNFMGMTAVERHKKLVNDYLTYYARGSDDLFGKKESGPIRTDADALREHHRFIRSDADNDDNSWEARLAKRYYDRLFKEYAIVDLSFYKQSKLGMRWRTQKEVVSGKGQFVCGAKSCEAVDGLCSYEVNFAYQEAGERNQALVKLRVCPSCAFKLNYRKEKQLQKASDTGAARKRKREQELEEQESRDPLVQEALEYVHRYVQRGSEAGPAAVRMEGIGSVDLEQAAGDVAPLPPPAAAVAGGPANECGRGETYGSGAGAGMSTERTQPAPAVITLPADIGERESKPVQETVSAAEMDGYFEGLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBR1</accession>
    <name>A0ABQ5SBR1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI66980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="850"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="933"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="976"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1332"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1539"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="543"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="601"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="624"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="857"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="984"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1332"/>
            <end position="1342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1905" mass="203960" checksum="4E41DFEDFEE84F67" modified="2025-10-08" version="1">MEVETFSENGESAGPPSAEPPNDLGKILSYDEIPEANGESSAEKVAAAFTTPRVVDKEAKLIDVAFLIDGADRQREVLEILRHKLNIIEDRVKGKHPGVSLRVAFLGYCSYPIVADLRQIGEGIFQEAVSVVLLRDKEEREGQDVLKGLDEVSCLSWSAQSRLLVHVSDFRGIPPGGDKRNNETDGSGTSLTSRNLAALQHLKECGLKKYYLAHFKAETQHLIEELRMSGIGEDGDGDAQWLVSERLYDPEVLPYKVAELVLDLLDRPTAGSLPEEPAEPTEQVRIRFPGAAGGGGGGQAPRSPSVASNFTADLKVTTPYSEVGPGQVSHRYRASSEDGAGPGPVTINESFQPTETDESSFRELPSRLPTGAGPNSGGGAIGDGPYVISSSDAGSNPSAFKGQRSLETVTSRSSSKSASTVPRNGTDSIRPYDETSSFEHGGSLTTGNIIQHMMMGRDSLSSVIDEVRSEVPSVAGYERRLSKADQTALGSGSSGKVTPKSRASSAGGAPDTSGHHARSSEIENTVNKGTRTKASETEVIPQAAVTSGTSTSASASSSSNVIPSQGRLTTSDAGSAHKSTKSSGPASEGGSGQVTPKNRRSASPSVSGVASVPTTGAPGQIGPSSEAHSFSARSSSVKSIPTPGILSIASDNSGATLPSQGTAAPVEVGSAPSIMPLALPWSNRALDDFKSEMRKASFKLLDPADEPLSARMDAVFALPPELWPVLPPGAMYCMEASDPRVTFGYLQHRDFPETKVRVNTYFGKWGRHLVDYVVSWQPTPRAEAAWQLADGKQPPLPWEQRQVVAHTTNGEYQYANTRMRFRGPKSAKPDSEVVEYSDAHAVDLLDTKAANETAPRNSDKDREAHLIDPPRPNTWGELARRPLIGNLRSRGGGTYVHHINYDHSSLRTTCGRRLPAGYFFTLIPNAVLKPPAVPGGGGGGGDSYTRSRPSSAGRVIAAVRQSRSFSSTIAESPSLLHRIRGTASRDSASTAPNSGSGAVRRSGLVKRGGGGAAGGPPMTSSPSVLSVVGLDVRRLVPKDRWRTYDVPFNHPIHSARAQELGADMAVASGRGVRTFSDAAAVLQVSEQELRLPRAFVPGPVVVPTWDDIPRLYAEHMEAARALMPVTELDTYGSSKLHMLIYHLQCAATLEYDRLDAKLLLLPQMARLGRKSAEIDAWLQATVEHASYLYDKLQIYDTSIWTTDSKDKPPQLLRSSLAALPPPPLPAATAIAATAPGQSQGWAFPDPLKGTAHASRSAVNVSVQRRPLLRQQQVAVQIVTKAEWSAVAGVMNPARHATLYDVWVRLRPVMTGAAPPGNGASYGSSRAGSMIAASSDAGATASGEGDRLPEPQDPYVHGSVNRELLDYVPKDLEAMSSPDFTPQAPAPAKLPKQRGYRGEGPLPLELVAEMPTVHRPVRCRGADETPLPPEYQTMDLWNIRHKAEQAPDNVPHSRDKGAVGASQVAIQRLKAVREFSSTHQAAVWKSMQLAKGVRPSDQSDAPYYGSDIAEEDSVTEINSEVNGGGANMPRRRVRVVPPRQDVSINGRPGAATQTEGDLGKPTPEEVARETQCKQAELEAAETRKLLTSGKLGATREQVLENALKAAEAAIESLKPRPIGYGINIPAEPDHPRLLFAADEPRDVALAYRPRPLEHSHPEVLPNHWYKRSDSISSQTATANEKGPEPFSLEKIWRDEHGSDHGSGVDAIPLTDLPTAKGGVFGDRTVAGFSALADYEVALARRRAIAGAEVVHLRANYIPKEDVFNLLVVYCNLDRHPDQVQMLQRAIDAPVSVWAVAAAARAATATAAASGPAAAAAAAAAKHTSVKLQAESEQEAIKQRIRSIAWLLGVTWRRITNETYTSFLSRVLGFPNDAMATLHRTRAAHLIARALVRGHDMNSVTKLTTRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC22</accession>
    <name>A0ABQ5SC22_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1084"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1330"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1340"/>
            <end position="1350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1350" mass="135536" checksum="0E7F4DC4685A3A26" modified="2025-10-08" version="1">MNTQDDRGAGGKAPRAPRVTAGNIVERMTSPLQRFFVNRGKAGASPNDNSNRKGAANLKASPPPSLNKDLRPELDQQTKVQGGSNGFSFPTLGGSNESPPTNRLTRPSLVAEIVAQPSTHDGVPFGPECGAVHAVATPGQLRAEDSQAGVGCQSTAAPLSSGAAAKSTLDRLTEWWSPSKQASLQFFLGGGTYFAPVSQPELPHAIGKAVSEPSRTSEQKGVYLNLAELLKAGPGGYVRKANGASSSAGAGVAMRYNPYSRPNAPRPPRPGATGLMPSVPQPVSIVPEETVQHRSMPEHRPSLAVAAARLPPPNPRFAPPPSGMAPRAPTAPGAVRLAPGQMALPSGGTGTPLPKFGSMLTGGLQPSASFKPVAGAVGGKRQRESPMVSPFPTSAIRTLSEFKKRRQTVLEQPRSVLAGLSARTPRPCERAPGAVPPGGSSTAAGTPGPTPGEVPQTETARKMIGFLNSCERTGDVAIATTTKAGSQSATRTAPPTYSRLPVSFMPPLERLDQNHYGGTPTPGVTPGEAGFACAATPSPLSNTPDAGQTPGPRMVLEFNTPTVIPVVESSRSLDNLPEDLASRVECVTNTLPLSPIVATANRDASLGPPIPPFLAVESTVVAKAEMCGRPDALVLAPLPVAKNDGLAQEPAGIELLPKFSFRSPHGLSDHQLLRVKEICETYSAAAASSSGSENFTFEAATTGVDTTAPIEKATSSGARTGNLAADEVKRASEVPLPDDDDDDVGVDDAAAKPAPVTTAHVSSEQPHALTALSVPHCGTNRVAFADQLEMMLARKQCDADAPLKATVAAMVATPPVTPGPVFFSSHAGSTPFHPFVADSTPVIGACEKSSGAVDKDASDAGFFSPISLGTATCTSAAAVVDVAKPSDTPPLGLGSASDSFFFGAVSKQTVPEGAVPLASAQEEKVGIDKTSCNAQKDTTTSFGLDKLGFAAPEAGSLPSASPGNATFGVAVADAPKPHMFGSYGAPSAASAAAAVSTVMGSGTAPTMAMPSFSVVGQLIGSTSLSTTSAPTTTCSGPMVSLGMASARGSAHTAATGFSLGSSTDVPASSSAAMTGFVFGAAQTSAGSNPPPSSFGSPMPIGNPASGPSISTGQPLASSVGFGTGSFGASSSAAMSFSLGGSVNVGTSNVSSNNSGPQYDVVKPVSTYAFTAPTSTPTHGAVTTGPTMDTSTPQPAAGGVVHTFCASAPAPLGAAAPSFSAPTAYPNLGAPPASNNPFLGAAVPAAAAQHASTGLAFVAPAAPTAAQPSFAGFGGSNGGAPARPFCQAAPSFGMQAQPAQAPMLSVNGFGSSFVAPGGLGAQTFGDAGGGFALGHGDPKDGRKKLKARRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCE8</accession>
    <name>A0ABQ5SCE8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046497888" evidence="2">
        <location>
            <begin position="21"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="434" mass="43871" checksum="3DAC591652C67B3F" modified="2025-10-08" version="1">MAQTTTICLLLLSTLLIATGADVSTTSLKPAESPEPARGPKPGPSLLGVSTSPQSNPSPKSPKPAESPEPAKSPKPAQSPEPAKSPKPAESPEPAKSPKPAQSPEPAKSPKPAQSPEPAKSPKPAKSPEPAKSPKPAESPEPAKSPKPAKSPEPAKSPKPAQSPEPAKSPKPAQSPEPAKSPKLGRSLLGVSTSPQPSPSPKSPKPAESPEPAKSPKPAQSPEPAKSPKPAKSPEPAKGPKPAQSPVPAKSPKPAQSPEPAKSPKPAQSPEPAKSPKPAKSPEPAKSPKPAQSPEPAKSPKPAQSPEPAKSPKLGRSLLGVATSPQPSPSPKSPKPAESPEPAKSPKPAQSPEPAKSPKPAKSPEPAKSPKPAKSPEPAKSPKPAQSPEPAKSPKPAQSPEPAKSPKPAQSPEPAKSPKPAESPEPVKGPKGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDD9</accession>
    <name>A0ABQ5SDD9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011912</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032862">
        <property type="entry name" value="ALKBH6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46030">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46030:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="9">
        <location>
            <begin position="167"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="263"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="411" mass="44424" checksum="05BE8849FD0766DC" modified="2025-10-08" version="1">MHVAGRHVEIPGQSAGREPKVQAYNIIVLADTEEAVEVTQCHGLQCQVSQLSVIFSNNFSTKFAMVSGDSGLTSGSGWYRKYRVAPHLPSLFYVPDYVVPEQEAALLREVRASKSKWVQLSGRRLQNLGGVVHAKGLIPAPLPSWLEPLLTRLAGEGGEGGLYGGKPPNHVLINSYLPGEGIMPHEDGPAYHPVVAILSLGAPAVLRFRKKPRPAEAVATEGGSDSCLGGPLDEASRKAVEFKSDHLHQHLQECQQQSGVQFQPLPAPQLPRPKQLPQSQVGKEYGQTQPAVSRRCESSAAAVAKAEAGSAAWESESAGRPAEHVSGHGGGDCRYSASLLLQPRSLVVFRDEAFTECLHSIEEVSEEWLDESVANLDMYGILPSTILPRGSERISLTVRRVALVLPSILRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDZ0</accession>
    <name>A0ABQ5SDZ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011567</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051341">
        <property type="entry name" value="Zyg-11_UBL_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12904">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12904:SF23">
        <property type="entry name" value="PROTEIN ZER-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="395"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="395"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="757" mass="82126" checksum="73850C5AE62B1FB4" modified="2025-10-08" version="1">MAQVVQTPPRERSLEEVFACVLPHLPPADRAALRHVCEPLRNLANSACAKLALTSPHVFYALATSHLLKRFSSISSLKFLFNSQQEVKAATMFLLTAQSDQAAQLARLTFGENRGAAQQLHQQQVQCGGLNGGYGQPESRFQQYGYNSGARTAPQTFDFTEPFLAMACCFTRTKAVTVDKTVLLETEAAALARLPSLEELRLDTNSSAQPLRRPLLQLMLSSLTGLRVLKLSYIRDTTADMVMSLPRLTHLTKLTLQYAYWGESGAGERVMGGLAKYQKQLAELYMYECGVSDDMLRLIMGITTLRRLYIQDEDAEEERCPSNKGLARITQLRRLEQLELFGEEFQLDGELLGRLAQLPALRHLAMAGLDSLQPDGEDSPAALGQTEDLGAEVEEMELSSTMGDDEEESYESLSGTSMGGDEATSRPQLSRGNSRCGAIGDMDRIAHAAPRQGQHCTALRGNFAQATVRAAIMTEDNSNCKHQPLRRNTSAQAQRGGDTAAVPLQILCRLDTLRLYGPSSLCASPPPLALLLPQPQLRNLKLSACASHANLRALSLQAGLSKLIVSHTAVPLLYPAMQLHQQPHPGASILGFHSEVLYGLASLQVLRIKDLAGFMDNHLSDLAVALCRLPSLEELKLRALGGVSDGGVAALGAVTRLRRLKLYALGDGVTEGGVAHLASSLPFLEELKVKDCRRVGPTLRSTVVLYRTTAAAAAVTAAGGQPTQRMATLSLAHPPVAMPIVRYGAAECEGGRDPRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE31</accession>
    <name>A0ABQ5SE31_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_012531</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="99"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="101"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="36"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI68187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="187" mass="20991" checksum="7B6301D9CD7AA4CB" modified="2025-10-08" version="1" fragment="single">AVCESYQKDAAAVIRDVATLLNEFETKLSLALQRDVGPDLDEGGEPGKSNRGRRGVKRRGDAAAGSEGGGLLGNVEPIHMRTILKRKYSAEVQKLQEEFAKRKKVGKLPQTAVTILKQWWATNLAWPYPTDDIKKQLCSATGLNHTQISNWFINQRKRHWVKLFNGKQPSCREEAETILRQLNVIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEM4</accession>
    <name>A0ABQ5SEM4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cation-transporting ATPase</fullName>
            <ecNumber evidence="12">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_012078</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="12"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="462"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1212"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1250"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1296"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1326"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1372"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="45"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="14">
        <location>
            <begin position="300"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="877"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="885"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI67792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1412" mass="150723" checksum="00142C71589A99DE" modified="2025-10-08" version="1">MTKRSSLVESKTVAAGDAVVMDDVGKPFTILLSVTKADDEEIDSFEGFGSPWWKKLVYYLVGVLTAGFSFLLCKWSPRLHILLSLSPCALKDAQYVRVRLADGRVDLERVQEVAMTEPRYDSQPTLAADEESSGRGGLLNWQVHKVHRLLEYRCTRYFYMDGTLAGPAGSLSGPTFTPVPAVPKGFNEQLKAAAQTLASTGSAAIAEMEQEWDLGERQLRYGKNEMTIPVKSIPMLIFHEMWHPFYVFQYFSILIWVVGDEYYSYAVCIFVITWFSIISAAVEAHNNMKRLADIAHFECEVDVLRSGEVVCLPSSSLVPGDLVVVGPSTLPCDMVLLRGECILDENMLTGESVPVRKVPYNPVADGLSYEPEKCPGCTLFGGTVVAQARASRNQKAVAMVCRTRFYSAKGQLLRSILFPREHEESFISDSLKFICGMLVLCLSLYVWAAVVLARNGASPSRIVVRFFDMITIAVPPALPACLTIATVLSIGRLRKKGIYVTSPDRITLAGQLDVICFDKTGTLTEQGLDLQGIVPIVDGRLHGMVANMTFLPMQLVELLASCHGLARMGEALVGDPLDQKLFLATQWDLVDERPSVDAAQANRSLGRVDSTGAEVVNGGIDGVDGAEAVAAVEAAEATGTSENVHTFVRPPGAAHAFSIIKRFEFSAALQRNLVVVRAPDGSVAVFAKGSPEAIRSLVAPASVPPDFDRLLGELTREGLRVLALAAGDASRVPESQLLGWTQAETETVVGLRLVGLAVMANPLRKDTLDVIEQLQYASIRTVMVTGDHLRTAVSVAHKCAILPAQRPILLVDAADTSLLPASPSPPSAAMAAVASPACIAAEPSLSPAATPLPIRISAPFPPGGFPSAPVMIQRPGTSLHHHTAAEATGPGPAGEPTPMGDPSVQVGKSWGAAMLAAGEEHVSPGSPVTGEPPDVGNGMATVSPQQVQLEVVGRSAAQPALAQTLVGNGAATMDKAGGSRLAQPLLASNSQHAFTAGACDAVAGPVGLRLSVLDVDGNVTETASVGMNSSGAGSIGISGLMTRLMMGELECAVTGKGFNCLLASPQLKLQPAQKGVPLLLPVLQRAAVFARMSPDNKRDLMLTLGGGIDGAPEDCPHLGLRVGFCGDGANDCGALKAAHVGVSLCEAEASVAAPMTSKAQTIASMITVVAEGRCTLMATYQIFQFIIVYALVQAFETNLMYTYSLNLGDYQYLIEDLFFTTVLAALMGMTAPRSGLSRRRPLSRVMSPPLMLSTVAQCGVVVVFQLASLWMLKAQPGYIRFRGDRDLHNTVAPENTATYVVALAQYVVMAMVFNKGMPHRSPIWTNVALLTALLAQTGFVLYTLFVTDKFNLEVQDMVNKNGVVDMPFRWKLLGLMVGMGAVAISVEYACMGLIKLMDLGRGSEQSKGHQIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SER4</accession>
    <name>A0ABQ5SER4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HotDog ACOT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_012268</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF8">
        <property type="entry name" value="HOTDOG ACOT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="6">
        <location>
            <begin position="155"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="114"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="406" mass="45368" checksum="424E9326D45F8C75" modified="2025-10-08" version="1">MMILTSGAFSFRKHISRMLTTLRLEEPATLKDASLDEARWCLPFSYEVGEHRRGGRGLLIRFKPHPDDPTDLSGPGPLDWALKARYERADAGIASAASRGGMVSTNIISAPEVSLSDNSAASSTRASQPHGTRGGPGKEFSSPPERAPSDSHLELIFPFSNNSTLREQYQRFTSNQMRFGLLLEDLDTMAGDIAARHAGVSERVLLTASIDRISWLQDPNVGEVSLANNLRMAGQVVWAGRSSMEVMIELSHQAPGSRPDEWRFIGLAFFVLVARTLDRTAAAEVPALVPETQREKELFEQGKVHMQQRQQRRDSHWARRPPTSQEVALVHDLIRQQHLHKPTSHHHHHQHQEAVSMHRTVLQNCCLMHSQDRNVFDVIFGGHLLRLAYEHAFSTAALYAGGNPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEY7</accession>
    <name>A0ABQ5SEY7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012885</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF519">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="117"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="502" mass="52719" checksum="17034AA99381D12C" modified="2025-10-08" version="1" fragment="single">MSGVNYYQLLGIHATATEGEIRRAYLRLSLLYHPDKRRIPDADADERYRSIQEAYETLTCPEKRCLYDFVGSFAALADLTSSPPVAMPIHASRHRRRGSAVPAADVSMGIWCRGSSGEALRSGGTSSSLASATRMSPCHSFSDETGRLSSSLGAPTGSSSSASGCLSATCNEPGGGGSIGIDGGGSPRGLPSLELIPPTQLDPNAPPPSSPPAATGGRSRGADFGGSGVGSSCSSLTSPGTLLGKRRVPGADGTQLQDLPPPAPWLAPDSHLPPSPPPVPQSHPIFSILSQSQPQPLVPPQPQLQPLAADPWVGSDICGSPSGRRVRSADVHHRLLLTLEEMYSGCVKQLRLARKVFRPAACMKLSAAAQQLPDQEDSAVPGGQQQQQLLLPRDGDLAGSSNNSSSVSQCAEELFRVVVQPGWREGTKVTFQGKGNELPCGSRGDMILVVVQATHGRYERRGNDLHIRVVVPLVDALTGGDTSITTLDGRTLVLKRGSACLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEY9</accession>
    <name>A0ABQ5SEY9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Diacylglycerol O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_012560</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF34">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="427"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="461"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="878"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI68216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="982" mass="104592" checksum="A9B518C9A14AD77E" modified="2025-10-08" version="1">MELLVQLVHAANAASRVVASTLHLCLCLTYAAHLYRHRRDWARGPILNTLRSLFLFPLMGGHRHTIFQVFAWGLWTLSAGITLPVYLSLLAADSRSFAATGLRNALLDFLGLRKPLIPLTHLDAASGAAAVSGLWGELFMLKALLVFEGGARFGEHGRDHKRQRSWQEKDIQDMVAADESTSGKPVVHDEEHRNRKQLVEGSLASLLVFVMGLALSVTGSVLLLALEHLPDTATGVLYCCLALSCISIGASSTHGLGGYLRHCAGAASSAGAAVLRGGASAAAAAATVAAGAMHGGSSGGGGSSSSSRVQSGGSADVAGAAAAADQEDEADVAPSWRFFQPFQGGSVFVATQAIGWTLFSSTVVAVLLLAKELVAGMGYCMRCWALAAGALMMATQLVLGISVLTYQNHRQARKLLQPMLHHPGASFARAAGLVMPVVMMYLPLHVSCSCMAAVFTLLPPGQATAVVLAVLLPYFAITLRGHPAHSGCRRWEWLCRWFSREVEGSLAWWAGRLVVIRDFPGTAHQVADELEGTCDKQQHHHHHQQQQQDMGTSMNSPGQTRRYVLGFHPHGLYPTGAGFLPLMPSIQAAFPGLTLVTLTASIVHYMPGLREIAGWAGFRQVTRATFRRALRDTGAVLLCPGGQAELVYADRAFPRRGTPAAAAAAAFTATAATLKLNLGGALPLSKPADAAAPATSVAPNTVTDPEVCPAGAPVIAAVAAEVTAPVPRPTGRQLQPRHQLVIFAGHQGFVRLALEEGAWLVPVLALGETLQLRNLISAPEMQRATYKRLGFPVPFILAGRWGLTTLPLRNPLIYVTGRPLQPPAGWEVERRRQLTGLQSCAAPAGEGGDAPGESRTTGLVAAPTALAGSRQRHWRYWSPQGGANHHAGTPIGAPRTHGGGGGSSEQGMVIVPQSLVDEYHCRFYAAMAALWQRYHDQHPVLRHAELLLEWGKHGPPPDFERYRRQAEEDQAARNDAVVAAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF89</accession>
    <name>A0ABQ5SF89_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013032</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF1">
        <property type="entry name" value="PROTEIN YIPF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="96"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="233" mass="25441" checksum="228AD73C1563E213" modified="2025-10-08" version="1">MWPLRSGPPNANVQPTPPPGLFQDVPGIPSTSTTMPQSPPPTIPTSTQAQSTIPEEYTLDEPIWKTIWRDIVTIARNLRSVLIPVNWKFHGQAQALRNWDLWGPLVFMLVLAIVLSVHETNASQVFAMVFAEVALGAIILTVNVILLGGELVFFQAICLLGYCLFPLVVAAIICASVKTKWIRSLVTLGCLAWASVATIPFIGNAVPRGRKALAVYPVMLMYVSLAWLALVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFP1</accession>
    <name>A0ABQ5SFP1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chlorophyllase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017395">
        <property type="entry name" value="Chlorophyllase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428:SF14">
        <property type="entry name" value="CARBOXYLESTERASE TYPE B DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428">
        <property type="entry name" value="CHLOROPHYLLASE-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07224">
        <property type="entry name" value="Chlorophyllase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="45180" checksum="04E22A8ED419519F" modified="2025-10-08" version="1">MRQSILAPATSRSISNRSHVLSRRELLNATIIAPFSSLIPEQPAGASQEPPNPTLAPRTCRFALDFDYLTSSQNGRNKALRVQLPIEVLTSTTSERADTEAPVVIFSPGFLQPAASYGTFLATLHDAGVTVVTYDKSFETLSSVVDDVDSVVLLNRVAAEVLVRLGPLAAGPLFLAGHSRGGKLSVLAAAAASHAASTATAESPVWPPAEPAAVADVTALRTTGHTRVDPEQQHQQHQQQRSMSVSGMILLDPADGSFEPQDPNHYPSALAALQNHPSLADVPMLIIGAGRGGDCVPRAKNFRAFFKACRGQCTMVTLQDAGHLQFLDRDSGSLQQSICAAGRGLSNSRVAAVGGKLAAAFLHQVADTSPAVMPKGGQPAESNSSNNALRGGGVSCSSSTIERLYEEQLREEGLRYEVQVSGCSKGLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG64</accession>
    <name>A0ABQ5SG64_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052980">
        <property type="entry name" value="Crinkler_effector"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33129:SF1">
        <property type="entry name" value="ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33129">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="114" mass="12942" checksum="DFD8F666450DC420" modified="2025-10-08" version="1" fragment="single">PGIGKSFFAVVLMGWLVQEKGVNTILLDFQGMKYLFTSNGTDIKVEVGSEMDFFDEVNDPKTWWIVDMATAVQRDASTVLLACLDEQRYSEFLKLPGTTTLYMPIWTDDDIEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGC2</accession>
    <name>A0ABQ5SGC2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="698"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="887"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="959"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1152"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1354"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1496"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1587"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1647"/>
            <end position="1822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="607"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="620"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="962"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="977"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1365"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1507"/>
            <end position="1523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1591"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1647"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1705"/>
            <end position="1721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1765"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1781"/>
            <end position="1792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1804"/>
            <end position="1819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1985" mass="209641" checksum="D799E1578DECB1A3" modified="2025-10-08" version="1">MAARLFKVGDIVAGVPPNLSYWWPAQVADISHAPEKLHESLNSQLNECKGSVILRYLGSPTFEYAVCEEQSVEFLRDESDVGLLFIPAKGKNRQASLQAIEEIQQLLVQRRQLDHTQMHQGAHPVCLDLSEQDTWPAAFRERALLSPPRALTPDDCTRTSPSSALDNLLAAARGGGRLLLQETMWTGLVYSWSVAKGKQFLELVRAAGGGGADTLSVQPQAGPIPKSEMEAASASDTSALSPHEWAVNADERPIKRRRVGHGAAAAVSRVQPSGHLAAAARPLANGGLAGEDQPLLPQHGTAAGQQSAPRPEASEPVLTMYWKHHGHVLHASELHQGERKEPEPRPQQLEVTTDAANTDKADDSWDDDAIITRQQRKAESGIEVDDADDDVRSTFVALQSWPQRGCRGKSHAEVRDNGQVQGVGMELDELKPSAPIGANQQPKDDEDLKLLAAGGHGAEIALPLAVTSSPMQIEPAGPQVGSCTALTGAQRAPSPAPTPVAADRMAVEDMEKRKLATGWGNKTAAEADVGAVSSAKVRAAAGDAEEGTKLPEAFPLAGHENGVKRSDERRAANGGGCKGDRNVVVDAAGRALGFQSWCTKAEERSTASDTTSEAATVSTPKRSSSQTPSWRRTPSRSLRGVQLVPGAALGINKIASCLPAETSAAARVVAPYSTETNLPVLNEGRSQSVAAPMAKTAVGADGDSTPAIARPASSDLQQSKAGAEPSGASIVVSDYQKCTTAAGPIDRKDKPYAAGLGGDGAAMGKGPIHVPGSTVAESGFGLQWQPVSELPARVEGYRGLHVSADDVSAGALKLLRRLARNPVAVMSRDYTPRPELVHRATLLLCRELCFAKSHLAEEGSLGPRVKKVLRQAGEWHDAEEQQHNGNLVSAPCKDMQPASQQQQPCPRKLQRKASAAAEGDTKGIATGAPQSFRRTAEEASGRGKAKGIYTTSLVGSVQPDATESRAMRSTADVEPKASAQGCASRGRAARAPSLGPAAAGGSSAFTRAPGSLARNGQTTPMAPKVAMSAVPPHLMASGCSLRDEGEVDLASMEQPLRCIVSITARNSSSGGVRAGMPFAQQQPTTITTSAAAAAASLHRRPGANPSPATSTIGGLQLSSLVSMKSATASRMHPVGAVTAVPTARLPAAVKPVVGQASGQAPARVPRPHAPPPAPSVMKPPQCRAPVQGEASDSNHLGSGGAERLLIWFGKGVPMPTQTTVRNVAMMVDRGDPTQRPTIEIDYSSMQRCASIVFPRGSDARLLEALLRADGGNLLKIPAMQNVKMEVIHPDAPVQPPWSRHLNSPQVGGQQEPLASVAAAGGPSGSMYAGLPPATVGDRSREGITVMSAKLPARPAQPTIGTQGHHTGHRVRKKQASTSTVPGRVTPAHHKGAMPVVQRNTVRGPTAEGNMVAISVAQQQQQQQQSHTTPFFVIPESTGVGRTPGPWPMGSSHTYMPSYEVLGTKYCEEEQLAPWLAGGLYSPTREEQRCQEEQECPLLTHPPPLGMQHQQKASKPKQDMTQGDPNKKDNFATDGAAGSVITSEQISTTNININTSLPFHMSCERRAGFPEQQLGNQQGAKQTLVPSSLIPGKEHDSFARGSLHERGRTDARGSSGDSRASLEADNAAMVFHNAGELGLGCDHACNGGRDREEEQLQRNLEPPEVHEQSRDHGWERDHNHHQEQEWDQKRNREHMGRTTRSRSRSTSREESPLGRAVNHDVKSQLPPTRPLVQDLIPPGSRIEFRPPSDGDGAPDSRAPSSVSPEAADTGGAGPATTAAVLREQHSSRPDEGKSGTVAAAVAPDSNRTSASARSTPSCNGASGLGAVPSTWEVIPPPEVELRGTGMPTTSGALVRSVSMMMAGTATGGPLQPPFKEQHQQVLEGQQQRPQQLYDTCVAAVEQPQRMPVEDSMALEALEDEGQEAAPDAASPNCAPVEALARAIDTEAQSTSSLAELLSRLSPGQVQALGSVLLNRMQDLEQPTSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGP5</accession>
    <name>A0ABQ5SGP5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33344">
        <property type="entry name" value="OS02G0761600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33344:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="29708" checksum="AF37E5A5DF094877" modified="2025-10-08" version="1">MQTSQEKPQSPWKLVLIGLLALACGFLTMNFHHILRTTHIHLSLAQHHKTTAFCAFAGCKLGVDGSAIPESLPAEKSAEERLAEEKLANTTNALVTRICGSPAVDGYAHVVPKCLEDSPTAKWWAEYYASGGKQADLVVHIEKQADYDGLAVYWGINNKKASVEECAEHCKKHMPNTVDGPFKALPCNAFAFCPDDVCFEPDAHHHTKGDCWLKFTEGPASPEVNMRGVLPDSYRRRHPQAPAAVQWQSGVLLPPGVTLTNGTWGPRWSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHL8</accession>
    <name>A0ABQ5SHL8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09212">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036339">
        <property type="entry name" value="PUB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153:SF38">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153">
        <property type="entry name" value="UBX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143503">
        <property type="entry name" value="PUG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUB" evidence="2">
        <location>
            <begin position="58"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="228" mass="25203" checksum="06CD297F2AED0379" modified="2025-10-08" version="1">MTEAKAGHTAAVGPNQGSPTALAMQQINHQMVQEGRRKFSSHLEDSVLDYLHNNPINIEACLATCNKLFGNVINNPDEEKYRKVKVASNTLKNTVMVVKGGEDLLLHAGWVPRVMEMEKYWVFDAAPDSVRFGVLKEALHLTERALHTVHEKAEKKRKEHEEKLHHDSAEKERIRLAIEEDKAERRQRAEQAAAAAKAAAAAAAAAPAPTAATRSSPTRRSPAGPKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHM4</accession>
    <name>A0ABQ5SHM4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013496</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="785"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="883"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="975"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1151"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1410"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1512"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1574"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="504"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="612"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="642"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="656"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="828"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1039"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1050"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1157"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1421"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1533"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1590"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1626"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1640"/>
            <end position="1662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1712" mass="178748" checksum="C14FA2B9EDD8637B" modified="2025-10-08" version="1">MEETSRKKDRSITWQAYLPSDVKQDILEIYERLDAERKGYITQQQLHTALNEAFGKPIRTETVRTLATRMDITSRHGLKISYNDFLEYMAQKLLSAESYDDLLRSEQGPLMQEVGYKVYELLERFKRKQMLKDAMAGGDGIQRLLHFTAAPFAHRNRRKGQLGIDLMSGESGTNGSKTGGPSVHRRHGGGGGSATRAEGGMGSRTIGDANFVSRSSLVSRRSSVTGAENHSSSLVQPSAGAPTRSSNARISSRPPHSHQGRNSAPAARASSALASLLSRADGPDADQAAGISVEDRGSAQRWRKSTSHTAEGVQPTAWQGQGDPAMPLKELNPPSGAAATTSGLPGTDKDPNHDASRLLSPHTSVPNTSGGLQPGGLQTMISVTVGETNPWPQKRLVPVSAGASVPAVATMPAPVSRSSMATSAISIPGRVGSRVSSPALNPVGVNGSVPLPTSPVGSKAEGWRSAALMAQVLEIERIDVHSLVMEDLRATVGLSTSPIQPRAQLRPQQSQPLLHQLSSSHPLQHPPSTLQLQPQQSQGQPQQPLHTRSALQGRSDALPLQPPTLEDGNRTCGVLTTDFDTCERSADAPAEGVTMRPHRAESHTQAPPSSLPPTQQRQPQRQPQQPSSTSVQPPVVQPPWSPQSRPQNSNPMLLQPQAQSQMQLKNQAQNGIAERRREGEPVGAWVSVDPEGALGLLLRGSLSSGGQPRNRPRFHAVSAPEALRLDVPVPIESDPLLAKLPDWVRKGIGLPPAPVVRVMESEPGAQPIPLASAPATLTVLSIVASEPPCGSDSDARQPDASSGRRGGRAAQPEAGVGHDDSGEIPGENDTSAISAQSSNGLRPMDSSGSHRPMVSRGFSLTRQSTTRFVPFGGDTAAVVAEAAEEGESGMAADSENSLSHEPSALEELQKTSNAVECPPIPEACSPRAQISSGLQQLHSPNNHTNPSAASAALCGQVIRQPELLTPGFTSFRKSVASCPGNPDASAVGPPVGSGAGDGDWLGLEAEAEANRESLAAWEESVLLPRSRDGHDTLQVGEQVSKEGHEREGQENDDGSDNDDETMMLYERLMARSMRSCSSLRRAPLGAKEITALAAHNEHHEPQVNAHGQQGAQQDFQSQASFLQQLQLQQAGNPQMPAEVEDSIVCEQSLPDEEQNRQRQPAAQGQKEVSASGQMEYLSAALDSRSPGVQVNGLQSQPMLQGLPPRRFFSQVRPRSAALLPSAAAVVSAASAAATMRSSDDGAQASGGSFWSADYVSCSDGAVAGRAHVRLNHTAMLRLHKKYGSELIAAAGAALGLGGSSAAARAVASRELLQALTSHGSGRIVGTSSFAAKRTSAPSSSSLPSPHYITAASTCAVLTTATVSAPVPSECVAQPRRTSSSTGAGEDLLCQTSGSGIYLPHDAVGLGRQIMRQKSQRSTESGAGGSCGGGSASGPNSVPRPASAKAPRNPVYRQTNATHDSAVIAAAHTQAGRVVTSPPSVACAAATSTNSPVHLQPHTRMYPAPVPRMPVASGSGLLRPASAAPNRQAPGRSASASIPSPTITIPRLRKMDESEVSSCFTTLVVANNVMADISRPAHRSSGRPSQPPSSVPSVRSAPIPTLSAQSPSMPAKGVGLLAGDRRAAGRTGSGMGSSGGGASSGGTSVHSGSASSSAGSRGTVVTVEGSSGEERLRRQLEVMGTVALLPAHSGCEMEAPGGIAPGRRIAAVLARSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHN5</accession>
    <name>A0ABQ5SHN5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Membrane magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013576</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane magnesium transporter (TC 1.A.67) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018937">
        <property type="entry name" value="MMgT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21181">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21181:SF7">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10270">
        <property type="entry name" value="MMgT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="11798" checksum="5CAF4EDCAE494C30" modified="2025-10-08" version="1">MVRVSTAGLLTLSGLAGLVHTTFAVIQYRQLLKLSQEEFEALPATIVLELCISTIVALLGAYILAGPIKVIVMPAKGRNMDIGSLRTDLVSFNMRSRAMPLDIPPLQPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHW5</accession>
    <name>A0ABQ5SHW5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013930</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF10">
        <property type="entry name" value="HISTONE-ARGININE METHYLTRANSFERASE CARMER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="520"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="701"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="536"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="565"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="706"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="952"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="952" mass="100631" checksum="1B4F896C2FF63030" modified="2025-10-08" version="1" fragment="single">MNITNDDALDVMVSIGRKALERGKVDQALTILSGVVSKAPKHALAWFSLGRAHLAKGSRDSAAMCYHRTASLAANQRGVPHNLQALARDNMVLIGEKVLPPNWLATMQDPKIADIWGTALDGLAAAAAVATRERQQPLHAVVAGGIAPQILVLQALLTAHAAAVSATTSVVDRDAPPATVLWIQPDELSRTLAAQMAAAAGVPSTKVRVVNGWNAAADDAGDAAAAEGTAGSAAGGPPTRAFGKADVLVAAGLCTPRLALHDWVTALAEARHLLSPTALLCPRMIRLMGALVASDDLIAMNEVRLDWMAEDTGGLQYGPANELLWRPARSMQVSVLRYSLMSEPFLLLPEVHTQELLVHPEQLFIQPEAAATAASAAVVVATGFSGAGVARSGARKRHDVIAVPSSSGRVDCVVCWTEYELVPGVWLSYAPHEIQGRTDSPALSPHIWQRVHYLSARPRVEAGEPVVLQVTLAIDGGDLRVEYDEDVTAARRLNGGEVHSNCRDESDTDGEELQRLPHGEALLPSGDSSEGSRDGQDDDEDDDEDEDEDDEEEEEAGFNARDADSSSGAGDDSAGDCGTAVVSTAAAATAAAAALAGSGLLLPYHMSMLNDRHRTRCYVRGIRGAVEEARAQHPDQQLVVLDVGAGTGLLSLVAARAGADCVVGCERELALAVTANALSTANKLTDRVNIVQLHSKDLRVAPEPPSSSLLQEPLQEAPASSKAAEAPDQASERCLGGPYHLPHKARLVLHEIFGTDPLSEHILPSMAQVQGSLAAPDAVYLPSAFRIVAALAHSQLLQERMREVRPPVELPAGPAAETEAVARLRRVLPAAAAAVLQPWAPWKAEVDLLRVPDLFILSEPVEVCCVRLDTNPLPRAFRAAATALPLRRPTPVSELFRRQGPDDDDLSLPPLPPPAAVGPVTAATSIVYWFEADCSTGGWLSTQPGSSCTYYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIH2</accession>
    <name>A0ABQ5SIH2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_014389</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF3">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="136"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="56"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="258"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="428" mass="47309" checksum="01FC6303D05251C7" modified="2025-10-08" version="1">MTDANKDEARKCLAIARQCIAQGQLDRADKFARKAQKLYASVEAQSILEVIESAKAAKETSTHERPSTSYGHHNEHPTRVNGQSATGTNYASNRNAEGGPNLPPKPHKSSKPIGQVNDPGTPEQRALVAQVLKAKDFYEVLGLSRDATDDDIKKAYRKLALKLHPDKNKALHSDEAFKGVSKAFNCLSDPDKRAYYDRTGFESSAAAAAAHRGSGAGPGPGGTAYYHASGEELDPEEIFNMFFAGAFGPNTFRTQFGGAARQRRQHGHNHAGGGRAHGAGQGGAAAGQEQQQRAAMLGLLQLVPIVIFLVYTLFQSSQSPPFSLTQDSTYRIEVLTQRLSIPFYVKTVTDLEKSYPTTSDARVRLERQVEHAFYERLEARCQQERLMRHRAWTWGNREQARTMKLDACDAIEKINEKLGSMRRQQYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ67</accession>
    <name>A0ABQ5SJ67_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014233</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF289">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE VIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="16"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="512"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="714"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="718"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="752" mass="79562" checksum="E386A32470D191A7" modified="2025-10-08" version="1">MATTPLTHESLLIRLKISNKILGTGSFAAVHRGTWDGRPCAVKVLLPQHANIPRDPTRSDCPAQMLEREGNMLMRYPHRSLVTCYAVLELRPGFPGLPAGYKGSVPALILEYLQNGSLYKLLLQQQQTPWSHMYDDVTAMSWSIQIASALVHLHGLSPAVIHRDVKLENVMLNREKAQEGSASGEAPNMSSSPGPRANRPSVKLVDLGLHVAASHVADEQLFRAAMTSSFCDSPSGTAGSTQSPFVCSVNTEVANSNLQSRSPPHFGRATYSGGGGGATSAAKTLRRCGTSTAITSGGYSGGGSKTSVPPLFLALVPGSSRMLRVDPRSPGLRNRNAALILPEQLTAAEAEGDTRAPSALRDASIATSFERFNSGTQRQFSGLASSSSMCWVAAAGAPHSRSETQPTRAFSYREPVTRAASGLRRQLPPPPPSPPQVTTAASHQLALQMRKLDKRIMKLAEVPPSASAGLVDEVLGVTVNAIMAATEAAAVTKAASRMATTMLTTVMATATTDPETAPSPLDTEGYGSSSNNSYSGGAGEAAAVAEDVCEGAETAMLRSKEWASITSPSMNCATARVMRLYESSVPVESVYRLTGATGSHMTMAPEVYQGLPYNEKADVFSFGVVLYELFARSLLLVTHINTRKPGLPAVMQKPGQYAEAVSQGYRPEWTSCIPAPVWDLITECWNQDPLARPTMAEAESRLREISSQMDSAVAASGGARGGRGGGASGIRSERSPRVHLGPSECLGGCVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ79</accession>
    <name>A0ABQ5SJ79_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endoglucanase</fullName>
            <ecNumber evidence="10">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_014117</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 10">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="10"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033126">
        <property type="entry name" value="Glyco_hydro_9_Asp/Glu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018221">
        <property type="entry name" value="Glyco_hydro_9_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00592">
        <property type="entry name" value="GH9_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00698">
        <property type="entry name" value="GH9_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 10" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044986795" description="Endoglucanase" evidence="10">
        <location>
            <begin position="26"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 9" evidence="12">
        <location>
            <begin position="34"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="568"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="577"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="502"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI69484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="808" mass="86022" checksum="3B2EC80608060DA0" modified="2025-10-08" version="1">MTSFRFGLAVTFLIVCLNRNSLTNAVQLQHNFSYVLELSFRFYEAQMSGEVPSWSRASLAQGGWRNRSHTRDGFGPDGIGVSLEGGWYDAGDHLKISLPLGVTVSMLAYGALTWEAAYRKAGQWDAAVQNLDWAASYLIKCHVNASDTPSENTFVAQVGDGGTDHNLYWGRPEQQPEGGVEGSTGWRPVYLLTAEQGTGGDIVSEAVAAMVGSALLLRRPGKLYNPQKADELLRRARQLFEFAKLLPNMWSPPAGHQAMYGSNRWRDDLTWAAAWLCRAAVETGAGGGAGTTAGAAACAEAASLWADAADSDEYYRDAIAWAAVFPLASMLLRDMDAGGAAMAWRYEQHISYVLNRWISTTTQCNGTSPEFQVCYTPGGLAWYTSWGSSRCAANVAMAALMSSRSNVGSAEVSKRQQQRRCWAWRQADYLLGGNSRRQSFVVGYAPTAYHSSPTRPVHKSSSCDRDYQVSCDWYAMSAPGPNPNVLYGALVGGPDQTDGYQDSRSDYEKNEVSLDYNAGFTGAMAGLVEVESLLQAGGCNWEGYCAGTCGTPADAGVFPAPSSCPVAPGEEPSCGAPAPPSPSPPPPPPSPKSPPSPFPPARPSLPPPSPQPPPSPPSPPDGLPTDPSGAPSMCRPYDAACRACSGNFNITNATACGACVAVVRYMRRNPYLCHECAGYGVVDNDIQNLCQSECVPQSLTRGTSWACSLICSNENNVGADLALARQCVDCVRGTTDGWACGRCIEATRCLTDWREARAQCFDCVTSGAFDALGCAECAAKPTPAARTACLSCRGVTGGPTAGGFTTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJL4</accession>
    <name>A0ABQ5SJL4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014346</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38382">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38382:SF1">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="338" mass="36957" checksum="53DF8FE1933A351F" modified="2025-10-08" version="1">MQRNIKGYAYIQARRDPSPAAGVAKDTGSCLLRLLGNGQQATVSSSNSSLTPAAAAAGAVHVASSVQLSYGATLHSVSNPAGGLGVGPDRCLCPSRIHDKPQEKVGKPEQLSINKHSNGAPALSPLYLLRAPQAAQLPLAATTAQTQKQPLLRQIHRHRWNNDPQPPVDAHEQPQGCHKTLDQPLSQLIQNRVAKDLSPPLCQQQSHCHHRQLHQHLKQLDQQHWHHRQDQRPVTGMPMSRQWQAATSTLEASEANGALLVLGGSRPSHASRKRAGTWMDGERPRVVQPRREQEPERRFNYWDDGGGTWDCDTLGIEEADDGGLRWEGVGTTAVGPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKP0</accession>
    <name>A0ABQ5SKP0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015479</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047441844" description="FAS1 domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="3">
        <location>
            <begin position="25"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="3">
        <location>
            <begin position="1070"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1039"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1042"/>
            <end position="1068"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1477" mass="156543" checksum="C357663C9E3F1228" modified="2025-10-08" version="1">MGRLPAALAVAVALLALVPGGHAQFATPYAYIEAQTDLTITRSCIDNLLLKNQAPWTSQKSLDYTLFLPTDDGWKVIFDYFGLNQSPEEFCSELRRTKLNQTRNAVLKYHVLSGARFRDQLPGDCPGGCQYKTIAGGRNLVDEITVALDPTVGTYVIGGNNDDYGDYQPTGATTGPIRYNISINGRVLHVIDAALLQARYIPRSSPPPPPPVTRRPPPPVPTARPPSPVTSPPPPPPPAPPLPPVSSPPPPPASPPSPPPPAPPPPSPPPPAPPPPSPPPPSPPPPNPPPPSPPPPAPPPPSPPPPPGASPPPSPAPSPRPPSPNPPPSPYTGFFSIYAFLTQRVYDMTLLKAMVDAGDLATTFNNSNLEWTCFFPNDYATTWTNTMVWTSGSKMLQDQVAFVADCRLNSPNSTTCAPAIQQFSAYARNTLLQYCVDRTGLPLITTSNWINATYATVQTFVSANTFSGSVDYWASLSNSTVRSLLLSDNSLTRNRNNIVAKSVIQVMDSFLTGVIAAPPPPPPPSPLPPSPNPPPPAFASITAAIAGLNELAVTRQLIALLNITAQINALLDTTCYFPSNYAWALFGANANDRGAFVNVSNNATSPPPPPPAAPGGVRRRLVSIVGENTLQFRIAEGVYARAIVADRRMLIAGPNSFDPTAFYDRLAWNVTLEALNNTYNVQLLKNSIIDSCYNPASAPNSGNAGLNTVVRLQDTGSLPLETALGWGDSTGLTVFKTTFSGNCSDNSTVGPLTGVPLKTLGYCSQCRLYDPAIDLYSNCVFVVYSVLTGGQSGFEVNGTITNVAYSRGRSPFAYWVPHDIVVGSYAQPRGYIQIVDRIVQSPMLPPPPPSPLPPSPPPAPFPPPPFTGLRYFLENTNGFKACAAVLFSAFDYYSTVSAFDSSGWTLFIPSDSGCFAALSARGKTVSSIIADGTGRSVVKNMFIPNTLIPTSSLNTSVYLVTDLGTFGNNSIGTNILTFTFPNISNAVPTPIVVNQRFPGFSAQSANITGIRDVQIQRVPTALVSSGVAGLVHQIEGLLFPDITPPSPPPPPPRPPTPPPPPSPPPPPYFPTGVSSYLSSYPELSILKTLLDCTNLTSVLDATLVATPGTLFAPTNTAFTAFLAANGLSTTTLCQPDNINSTIKVLKAHIISSAYTSTDFIARTSSNSIRYPYLYAAELNNLQLIVTASASAVNVTAPMPLTTAFFVAGRFDTVLFSTSRPATPTSTGVAMAHMISNVLPVPQFSPPPPPPPPPPNVYNATTIWEAISREPLVATYAGLLQAITISPSSSSTFRNYLENPATIATILAPTVDALAIFFTRVQVDGNPLQYTDCVSGTNAKSVSICRAILSFSIFPDTQFLPSYNSSVYTIPSPIPGNDLQNFAPINSLTLAGNVTTGIAYRFNTTGSPPSVSSLGFATTTFCSGKPRATKGPVPVGIPNVITQQQSLMYLVNDVLLYSSALTSGITYTATATCTTPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKX7</accession>
    <name>A0ABQ5SKX7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014841</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase H subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.150">
        <property type="entry name" value="V-type ATPase, subunit H, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004908">
        <property type="entry name" value="ATPase_V1-cplx_hsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011987">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038497">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11698">
        <property type="entry name" value="V-ATPase_H_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03224">
        <property type="entry name" value="V-ATPase_H_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032184">
        <property type="entry name" value="ATPase_V1_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase V1 complex subunit H C-terminal" evidence="6">
        <location>
            <begin position="355"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR032184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="477" mass="54038" checksum="8A3CDCC75FF0056B" modified="2025-10-08" version="1">MAYSYLSDDSSPETSQAWAEILSRDIQWDTYATARLITDKDLQLIRRYDKRSAELKSSMLDESGPAYVEAFMTVLKNVTKPETVQYVLALLIQMLQENPSRARLFHQQSDQHLSAQPDPYTVLERLLQRQDDWWSQDKACKLLTIAIESRPRKSAGVANGASTSDGVAYAAAVDPAETHISLFLDWLVSQLSNRANLQKNPPTVINTCISCLAALLKERGIRQLFLRAGGLQALPAVVQRAKDSPTSSQLLYETCLCIWQMTYLRAAASIMSQVGLLKLLVEVCRVAQKEKVFRMALASLRNLLTYDDLCLASDMVEAGLNKVVIVRQLQSWGDDDIVEMLGFVDEKLKEGIVILSNFEKYKKEVMSGQLDWSPMHSSDLFWRENVEKFEERDFQVLRVLLKIIETNRDVKTLAVGCHDLGQFIVNHSQGRYIVNDLRGKELVMRLLSHSDAEVQKQALLCVQKLMLSKDKLDFLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKY7</accession>
    <name>A0ABQ5SKY7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial substrate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_015316</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="12"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="105"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="210"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI70454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="33271" checksum="0C9BD99100FF504F" modified="2025-10-08" version="1">MTSDRQDRHQGSKSQYVFISGAISGIVEGLSIQPLELLKTRFQINPGQPLRLLPTVREVLREGGVLQFYRGGLPEIVGLIPRATAALSTLEFSQRQLRRSSGGVLSPGGAYLSGALSGVSEGIAFAPFQVIKVRLMAKEHLGRYRNTWDCLAKVVRQEGVLALTTGLGPTLWRNCVWNSLYYGTMHQLEDPREGMGLLPAIENPVLAAARTILVGTAVGMMATCFNAPFDVVKSRFQALLPEDRVARGYRFTLPALASIASREGPAALYKGFVPKALRLGIGQTIGLMVFQRSLKAFGIDREQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLL7</accession>
    <name>A0ABQ5SLL7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="2">
        <location>
            <begin position="203"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="729"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="919"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1117"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="731"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="751"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="767"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="927"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="965"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1198" mass="123587" checksum="CF459BAD6177D9A7" modified="2025-10-08" version="1">MAFDLSKLEDWSSSATVRNPTTWVAFETTTQNQFIKAKRRKGARGRATTAEMAAMLESGGKSCRQEQILRPEQIEALRLKGEPKDLSVACLEPEDGEGNVEYKYRIKDPHPVRLQQLITQMKYRLSEGNGECFYYIGIEDNGYPKGLEPAELEASIGTLQRMAASLDAFVTLVHYLPAAWGRRAALLRVATSTAQEACHTDLRIAVAGSVDAGKSTLVAVLTHGSNGRPILDNGRGLARMNVFRHKHEMQSGRTSSICHQLLGYDRDGRVINYMTPAATALTPAEVTSSAAKVLTFLDMGGHEKYLKTALYGMTALLPDFGLLCVCAVGGLGRIAREHLAVAVALEVPVAIIITKVELVNAERFAAVLAEVRQLVALTMCHESSPGTAQHQHQPRTVLESATDERRVEDASPLVTTEAEALASARVLCQLHADTLASPTARGAVGVHMPVFAVSAVTGAGLPALHAFLNALQSPALLKAACHTPLATAGSSNGLQSSVATGASVSLGADSSCDGASSCDGTSAGGSPLPAGGSVEFDSLDTPSVASAAAAAAAAAGPQQQLLYAPAAAAVATAEAQAVHFQVDGTFEVEGVGSVVSGTLVSGMVRVDQELLLGPTAAGDFTRVVVRQLQRSHVQVKQVRAGQTCTIAISPAVVPQPVASSAAAAVATNRGAFSAGAKELPLGTSPGMGPMAAAAAAASIVADGDHRVSSSGFEDALAAAQTALELCLAGGSDEDDGGGDDDLFGFDASDGPAPPPARRAKVPPPQPSLGSAPQPTFRGSSFDDEAISMSTAMRYLHSTTSFCVGGEAGAGVAAPDATAGSAATATPLSTSEPPPRTFSLGATPSPFTGVASRSKGAVLLEPALQPRTSWTFEAVLILLGGHWPPRGLVSGRWPPAGNDEVDAAMQELELRCLLDPQTAAESSQLLQRSGSSNGPPSSGRSGSMTPASGGAADGSEMAIGEKVGSDGDGSGDGSASGSGVKRDTDLLIGALSDVGGSCGASGVAAMTTGREHEHIVPSRVSNGGNRQTAALGGDKSNCRRRRQRAGIACSYVTVVHCGSVRQPAQVLMVQELIDDGPANEPRPDGASSPYSCPDLRISASTRAAAALLHSASVAIPAGQLHPPLPAKTTDGSSNSTSMSSLQQPGAQQPVLDHDLGCLVRVVFRWQHRPEWLREGAKLLVRDRGDGRVAGAGFVRRVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLM5</accession>
    <name>A0ABQ5SLM5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_015289</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053879">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056307">
        <property type="entry name" value="Ig-CFAP74_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056310">
        <property type="entry name" value="Ig-CFAP74_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF012200">
        <property type="entry name" value="choice_anch_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22538">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22538:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22544">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24778">
        <property type="entry name" value="Ig-CFAP74_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24798">
        <property type="entry name" value="Ig-CFAP74_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24771">
        <property type="entry name" value="Ig_CFAP74_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFAP74 third Ig-like" evidence="9">
        <location>
            <begin position="925"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP74 fourth Ig-like" evidence="10">
        <location>
            <begin position="1045"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="8">
        <location>
            <begin position="1570"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="60"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="536"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1210"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1733"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1912"/>
            <end position="1965"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="267"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="555"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="575"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1279"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1304"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1733"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1916"/>
            <end position="1928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1929"/>
            <end position="1954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI70333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1965" mass="207553" checksum="64828E2FE9D9E2BC" modified="2025-10-08" version="1">MKAEELYLEELWKEDEEEEERLPPALHQDMVQHQQDNALPSYHELMGSGAMAVSLHHTKAATAAAAQSPMRPTNRRPSSSNGISGGGGGVGDESPPISPVAAWEEPPRLALEPTIGADPEQLKRRLQSVVKAVNANQERYEEVLLEANKATDLVRSMEAEISSLQREAEELSRRLPPAPERQRQVETWLSQRSAALEAQKGVQSSRQLELIAAEAALRAAERELDYLAEAKGEVEAAQASETANAEATANELRAREERAVAGYKRRQATEAARLAAAQQEAEEASRRRLEAAEEARQKVLARMASERTRLREGVAANQQRLVSEQGRRREAVLGLKSSMAAVRADVARQAELAAALQRQRREVQEKEFGALVEAGLNPYEVFRRRDQQEEAKRQHANIQSNIASRQLDLAAQLAREQEAYQRALAARQKGKMDRVEFDREMGVAARQARTDEYMRTHTLAGTTMLDPTGRLPVFPSQATVVKDARFGQGTAPPELLSKQLARLGPEVPKQLLLPAKYRNAEADAVAGLGYGRGGYGSGSDGEGMGSPPRSPHRTSAPAPALAAQQSQGVKHGLPSQHSHQQLQQQQQTAAADRYKQLATRPLTKLEQSILEAAKARHKQGVGAVQTQMGRTFGGDAFLASPTVIDFKDFDMGRTTTLSVQIINRSYKKNTFRVLGIPVEYCDVLDFSYKLPGYLAPGVSGEVAVSFTPKAAVDIDTCLQLLADTGSFEVPIRCRTKRAALSVTPAAVDFGGGVTLGDTDTRTFVLSNDGALEVEYRLDSPQLTDDERAMIKSATFTHGFVDATLAAAAAAATTATLRTGGGVGSSGTLSRARSFASLASAADGAVAPAVAPREERRLTHGGFTVFPCVGTIKGYSKVTFTVTFAPVLPAPTKLVLNFSYKAPSVKRLSLPQHEVVVTGVGRDVPIYLERPVIDFQCCLMGHAYRDVLLVRNGGKTAMKVTVVPRPELEGFFEFSPDFGFVQAGGSIPIAIRFKPTPALLTACRRHLVDPEEDQIIEVPMRLSVPDQTLPVPFSLRAQITHTDLVFEPPSLDFGDCVMAERTALRLIIRNTGRLPQTFGFVGLPSGLRVSPNDGFGHVLPGESLERIVSFQPPIAGPQSFSITSKTLAGRSFTLPCRAVGVSPRLSMSHNRISMPATAVGDTSTVSVVLINRTDAPQAYEFAVPPRSDLIFSPHVGEVPPQTRLRVQIDYCPRPPEEGEEPGAPPPPTDDDGNGPMYDGINSPGSPPRWPGLASPEAATSPPGGVSRDEGNEAAGPGAEGKARGDDSPGRSKRSSSPSAAKGKRKSNDGGKRKSSDGRKGGRASPSQSRKSGKVSEAPTDFNDLPDGADPDGAEYGGSGGAAVSGSAISTLWRRLYGAAASSWYRYREHTITCYIRPQQQQGPGGPPAVAAAAAAATTTATATGAIASSTSAVTTTQELHLQLSTCATLPDLVLYTALPYVGLHRCHCLDFGPVPVGQRVVRTLELSNLGAEVMTLTAEALDSRGVFSNVNALRPVRPGAVFRMLVSFTPHARTQYLETLVVRSARTKLRLALKGAGIAPELQLGPDGVTSNGLDLGDVLVGESGSRTLTVTNVCPFPLSFTMVVIGSGAAADPNLAMRPPFTCHPAEGTLAQGETAEVTVSFSPNSQRPYFADVLQVVVPNQQDELRVPLQGRGWQEGVFVAGTSYPEPDPDPFLLQQLAELVRVPLPPSVGGKQRGGGAVAAAAAAAVAPTAAPAGGTPTASKPNTPAGGSKAAKAAATTAASAAAAAPSEPRVLTLTFPDAVYAGETAKASFEVGSLKSTANGSAPGEVLLQELPAEAREAGWAVADPPAPSGGGAVKVPLAAGELRSITLSYTAPRQPHAGMVAAYGHAEFRTLRLQCTLRGGLVAISGGPEGRSLTIVAKCKLMPGARPGGEPLPPSVTAPPQPVGGAAPPGSAAAGVRKSVAPSAGGSPPASPPVAKRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMA8</accession>
    <name>A0ABQ5SMA8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GBF-interacting protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_015591</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009719">
        <property type="entry name" value="GIP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051833">
        <property type="entry name" value="TC-DDR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16308:SF13">
        <property type="entry name" value="PROTEIN LINGERER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16308">
        <property type="entry name" value="UBIQUITIN ASSOCIATED PROTEIN 2-LIKE/LINGERER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06972">
        <property type="entry name" value="GIP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBF-interacting protein 1 N-terminal" evidence="5">
        <location>
            <begin position="8"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="44"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="445"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="611"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="918"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1002"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="251"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="453"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="626"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="940"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="957"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1032" mass="103576" checksum="D2D28D4A91F11D85" modified="2025-10-08" version="1">MAELKPTPRKMIEAMKEVTGATEEDIKSMLQLCGGDVNMATERLLESPFQTVGKKKDRKPREEDRRNDSRPSGGRGYDRFANGGSGRGRGRGEGGRGGRGSGYATGRGSAGAPPPRGPSAGPRQGNSSHFTSDSNGAATHADSGWPAPSASDGDGNAWGAAGATPWGEEKAPETSTSNTSGFGASPAAAPSASGSAWGATPSDNISAPTQHPAPIRPGGGITMAERLKSSQQKPPAPAPVPAPAPEPPKPVALAAAPSPTPTGPAAVESETAPDVASAVEPTTSTNYAAPSVSTVASDWGANAGKDLMGMLAGASKAAAQAAPAASAQAAAPAPTASPSPAPVAVPVATPVVVAPVSTDKVVTLSAADNQVAPPSAGNQYASYVAAPVPAAPVPPSEVPKPSVPTPTAAAPPAPEVDSLQLSFGTFNIGGGLGDFGANFSASYNSQSAAEPAKPLPQPQPVPQPQPQQAPQQVVSQQPQQAQQVEQAAPVSQAATPSVALADQYKHAYAQYAMQTAADKAGVGQAPATNTAAEQPSAASSYGSFQSQAAAAFGGAFGASSFGAAGQVPEAGPRVFTQTDYAAAYNQDVLKQAAQSVYGQAPAAQAASAYNAQQQPQIAAPSPPPQQQQAAQQPQQQQQVTAPQQQPQQMQQQQMSVGQPQVGVSQQPHQQQPQQQPYATHQASQVQQQYVAQYAAAPYAQAQVGQPSGASAAAYNQAAAAKYTPTAGMYGQTDLGGVAASSSAAVASTVATAAPQVPNAQTATAAGGMPVNQYTAAPGVIPPMQFNSAGAGASHIPYTQAYPYNNPAFAAMYNNPYAAAAVYYQQMGYNQYQNPAAAAAASQPQPYLQANQHMSQRSAFPNYSTNPVVAGAGQLPGAGYNTNPGTGYSLTGYEDAGTATASAANATATAGAAAYNVPGAAGVKPADKQDPSSLGGYGMSAQQQQQLPQTAMQQQAGGQPGGQGGHMGHGGQHHGAGHYGNQAYGGYGNQGYGNQGYGNQGYGGGHYGGGGYGGQRGGYGGYNRGGPGNYNRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMC0</accession>
    <name>A0ABQ5SMC0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="590" mass="62976" checksum="80ED7BD2CED447CF" modified="2025-10-08" version="1">MRWDPSRRAGNQTASSLPLGSRRTIPAAGFTYLRPEVARSIFVDYSSENGDLEGNNNLYVHEKKTILQRAASWAPKNSRWIFLIGFILAPLFIVAVVLGAVCGTGHCGNGRNLDSAVMRSGPQAPPPPTPPTPRPAPPGTASRKAAPIQPSQPIVVPVSPSSEPQPLQELHHPSFPEGPSPVLAWVPAYSPPLVPSPAVYSPPLYADHPQPPSPYGGEPPLPTYSLLLPTYPLPPALPAPPGLPAPPALPAPPALPAPPALPAPPAFSFPAPEVSPKAPKPEYPRLDGTPPKASIPVSNTPSFDEPFAPAPPADLPPESTFTDVPDTFSQPSHPMPMEALIPSPVDSYPLQSPPAHLPHPSYPPAYPSQPVYPSQPVYPPQPAYPPQPAYPPQLAYPPQLAYPPQLAYPPPYPPLVEPPRQAYPSYPNVLPARPLPANPSPVYLPPLVDSLPVHPPPATPTYTPSFHAPPPFSGMPSQLPALTHSPPQRPLSGPSQPKTPRPLEGLPSPPARGFPNWPEWPQWPPYTPESLQPYHPASHDTPSLPPHVSHRSLFTSNQSPCVQPSSVELPNADPQNLDGAAEGVEELRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN61</accession>
    <name>A0ABQ5SN61_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015847</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734:SF3">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046652057" description="Brix domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Brix" evidence="3">
        <location>
            <begin position="180"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="402" mass="46684" checksum="3C9C4138C48A4AF0" modified="2025-10-08" version="1">MLMLLMFVVRSHIGWGLTCKVGIQAAIGSTVHRRHHMSAVFTYLFTREREWGQHSTEDCSKSFRFLVHRVAQYEDVTPCIRLTALQLTYQRSKSLGTKQAHNQTTPSALQKTKTKERQKRQKEATRAEELGLEPPPKPVQHTLENTREADDTMVDPTDEEVMADENDDEFAAHFANARPPKVLITTSYKPSKIMYTFLSEMLELLPCAEYYKRNGFPLKKIVKFAANRGYTDLMVFNEDRKQVNGLLLVHLPDGPTAHFRLSNLKLGRDIKGHGRSTSHKPELILNHFDTRLGHRLGRMLASLFPQDPQFRGRRVVTFHNQRDFIFLRHHRYIFEEKETRDQAAKDPAKAKKKAVIARLQELGPRFTLKLLSLQKGTFDSRNGEFEWLPKKENKTSRRRFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNH6</accession>
    <name>A0ABQ5SNH6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016759</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045984525" evidence="2">
        <location>
            <begin position="18"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="166" mass="18117" checksum="1DB3E19BF0E48688" modified="2025-10-08" version="1" fragment="single">NNCFMLLFLSCTKYFIATNPQKNGGNDEPLETHNGRPSKSQKQPAHGEDASMMVLAANPVGIHQKEKEVKATRRRQAKSSQPSTTRNSSRKARNSSKAAAKKVTNDDDVQPSTKVKPSTGTKRKVPLEVKSTDDDDSYERQKVKSASEGVQCTRALRQRGSSGGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP19</accession>
    <name>A0ABQ5SP19_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_016929</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="42"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI71650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="346" mass="40260" checksum="7945F0A404FD8F34" modified="2025-10-08" version="1">MPPSHEAVVHRARIYADVCTEKEPEYSDYEQLSINWGDQDHYEVVRKVGRGKYSEVFEGVNVAGEAPTRCIIKILKPVKKKKILREVKILQNLQGGSNIITLMDIVKDPHSRTPSLVFEYVENTDFKVLYPTLTDLDIRYYIYQLLKALHYCHSMGIMHRDVKPHNVMIDHSRRQLRLIDWGLAEFYFPEREFNVRVASRYFKGPELLVDYQLYDYSLDMWSLGCMLAAIIFRKEPFFFGHDNYDQLVKISKVLGTPDLHAYLEKYGLELDPQQEALLGTHSRKPWTRFINTDNQHLASPEAIDFIDKLLRYDHQDRITSKDALNHPYFDPVRGMSGGVTEAAAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPR1</accession>
    <name>A0ABQ5SPR1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="486" mass="51710" checksum="E607018D2D6BE5DC" modified="2025-10-08" version="1">MERLLRSTSLSCSNSGREPPRAHGQAQPWPPGYRPIQTYFQHLLCNGPCNHRFRRAHVPVCSQSPRQRGGDSQNEDANDPFSSSRVRITSSLVTYGLSGTLAVVGAAVAHIDLFGQIRWFSTDDLGLGLTSASFLMVLEGLLLAPPYDRLLAGSGRPGAKAGAVGLQLRTARVGELLSADSVAATLALQQACLVAAQVVRRAGLTRTSALLEGGLVLVREASKELLQRGLIFTFLAAWLADRAFEAGADDTLLLPLPAALLGHGSGSDAAAAMATATAMAGVELYVTDAARYLAGLLVTASVMPAVVAEAEAMRIGVASNLALVMVEEQQEAEIREQWRRRQKQQQQQQSVAGGGEGLGGQQQEGSTAEAYDEDLDSKETEAMLREYRLYDALGRCTATGPPRLTYGLTLLRGLLRFGAPNLVYAFTGNLAASFLSSALPALFLLGYVLRGPKVLLETSSATMPSSPEETEGQAIATRTRKDTSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SRJ1</accession>
    <name>A0ABQ5SRJ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016978</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000117">
        <property type="protein sequence ID" value="GLI71706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="18293" checksum="01939B10D21C4205" modified="2025-10-08" version="1" fragment="single">NEQITKCNSLQERFANVQKSMKLYRFQSRRLKRLLKEAHGQALDAADDYIFLNDEDDVHMAERMIRFEEPVQAESVSAALEPPAIAPAAIQPTTPTKLEERLHYSYKSRGSKSTAISAPCSWGIRDTFVEVFGSRICGQASIAAPSKSVFVKLLEAAAGQPSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RL54</accession>
    <name>A0ABQ5RL54_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000002">
        <property type="protein sequence ID" value="GLI58389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="231" mass="26753" checksum="B8FDBCBEA42861BA" modified="2025-10-08" version="1" fragment="single">EQQQSMQSSGFEIVSALMNVSRVEDLSVVTATDVVRRDQSVIALRPLHAIGNVKEDEMAFALAEAAKAELAYGGMELQQITNVQQQQQQQDIQLKQLEQQLNERDQQLQQQVDVKLQNVAQQQDGKLLEIMRQFTRQQDEKMRQFTQQQDEKMQDFTHGSRMRKCGSSWCSRTRRCKTSHGSCWMRRCGSSCSKLRLFGRTRWPGLTMSSPLMLTIPWSPCWWSRSRHSMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLH8</accession>
    <name>A0ABQ5RLH8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000219</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047046326" evidence="3">
        <location>
            <begin position="20"/>
            <end position="3658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2767"/>
            <end position="2789"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2911"/>
            <end position="2933"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2970"/>
            <end position="2994"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3015"/>
            <end position="3032"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3084"/>
            <end position="3110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3143"/>
            <end position="3160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3197"/>
            <end position="3216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3222"/>
            <end position="3244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1714"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1814"/>
            <end position="1836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1860"/>
            <end position="1881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1908"/>
            <end position="1951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2794"/>
            <end position="2901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3321"/>
            <end position="3380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3489"/>
            <end position="3575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3611"/>
            <end position="3658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="497"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="540"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1938"/>
            <end position="1949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2813"/>
            <end position="2828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2862"/>
            <end position="2878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="3499"/>
            <end position="3508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3520"/>
            <end position="3537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3658" mass="381315" checksum="223AA07C6B866851" modified="2025-10-08" version="1">MLVTEVFLVTLLSLLHVDGARERPWCTGGSALCSAVTPESTYQHTSLTAGNGLVYTLGGRKGGGQDEIFIPDQLYELEPKTMVLRSQPLSGWQDMQGRAAHSMVEWVEGSGRRHLVVFGGVAYRPSDQATAQEVLLNDVASVSLQTFAWSPHKSTGPAPPPRKFASAAVWRSDMMLVYGGQLANESWSNELWAYNLTDRRWMGQIYSVSSDSAPSARGYAGLAVYGDLLLLYGGYLTKLVSVVAPSPPSPPPGLPVGPNPTSGGSPSSSPNSNPTSGGSPSSSPNSNPTPGGSPSSSPNSNPTSGGSPSSSPNSNPTSGGSPSSSPNSNPTSGGSPSSSPNSNPTSGGSPSSSPNSNPTPGGSPSSSPNSNPTPGGSPSSSPNSNPTSGGSPSSSPNSNPTSGGSPSSSPNSNPTPGGSPSSSPNSNPTPGGSPSSSPNSNPTPGGSPSSNSTNSPPSSTNSTAGSSGVPGPLPAGNCSVSTLGSDCTANAPTMPAPQSNGSVPSNSSTQSTSTAATPPATGTPGSAISPLTPSTAGHSPPNPQPPSPWQGAPHPDPPSPSPSSPSQALAQPPSQVLPAPKLASTPMQPAYRGSRRRGLVQGSGLSPTAPTPPPPRQYPSPSTPAPTPTSPPPPVYITMKKYVVYKDLWLLDLRPLVATNNVTNTTIGSGSGSVLRTNSTSWVQTKLDTAPYQTFISMAAFDVSLRLNEEPGTLAVYASGAVKPYGYDLESKWRQWYARYTISNLNDAITQMDQGARNVTLFLRRRGLFQHPDLASTPTSSCCWVDVEDSPVDLDSGSGIVTFEAGLLSPPFVREQTLAVTADGDMIVHGGQRTADDSPIYMPTSRRLLLRWSNGGVTALSSASLKHAYFMSSSSSVIFRSMHAIQLPSSLSSSSNVTTDDSSVTSASTSTGIIFFGKRDTSVVSVPELSFAILSNTSAFAEIATCETGSGPCASLNVSGSTDSAPKRDAYASAPFLVPYSGRKYGSSGPFQVKTPLVLIYGGYATTSGAVTPASSDTSISWLAAQIKTALIACDTTNTLTTTQWGTYATCAYNKLNVTCTSGSSSSSTSSTSTGSAGSAPSTQSAPSTQSAPSTQSAPSTQSAPSAGRRRLYTRPQHQQRVGKSYMEEYEAIRQIVDIYDQGDDYDNLNAEYNSYGTRSSGRSSRRFRSRGIRGGARRLAATGEIDTTAKTYYNSALLVAWQDASWQPLTAQSMGAQRILYDVVTNLQGGTTHSPGPRVAASMSAIADQAALLFGGYTFDYTAGMDDGSVSSNEPLLANDVHYIRFYAGGDAVNSTSYGAAQCPPGASCAPASPVQWNPVQPLPQVAPLRQPKVSELPTSVNSFACAGGTSVLVEAMMSLSDTSSSTLGLTITTWGGNVLFNLDKIVEVLDGAVVGPLSVITITTIELPKGLIQVWLTSTEGRGWTQDEDYTTTRASCCTPDAVPGMPESQCCPQVRLSSVNCGKGKTYLQGFGPGEATFDVGSVNRAASRSNMTFFQYRKGHGLLVQPSSETLPTGEAASYTQFPLEQAGSYLHTFELQPQISSPRPTPRCRHASVFLNTTGVDKVYGPNGILVVYGGSSTITVSNATTRLSDLWVFFLSNNTWAQLHPIGTLPPALIDHAMVAVGTQIYLGGGEYFNTTNRAFQQDSTVYLLDLGLSTMYWRKLSGGSTLFTGGTSSTSSYYSLDYVPELNRLAFSTPKDLLFIPITSRSSVSTSNASSSGAGTSSNDTAAASSNISTDSSVISPLLSSLYDGDTLLLSDLSSITVAPGEPLSVYAAILIQGRYAPPSSGGGGGASQQRQLLMGSLGAGPEAEALLGPDAAPSDSTGSSREEDFKFSGTVYRLSLIEDNQVSLRPEYRNQDSRSHSHHSHLLQSRPLSSQYHQHSNSVLWGSAVSSRQVVEHATGFNTENGEVDSQGGDRGLAGGRRLRVTSAPPASPSPVPPFPSPATTSLEDLLSQYLSGVGLTEIDCSGADRAMTIVSGGVLIENLLFRNCRDTAVVVGSPSGSATVRFSNCVFFNNSGTLGGALRVGSGASVVIDSSLFLGNDATQGGALFVEGGGQVISVTRSIFTANGNGNRPWNRQGGAVYGDSGSCLQSFATCQFHSNGDLSTITTTTTTTTTTTTTTTTTTSNSTALGGGMLLVRPSCGCSVSDSLFTFNKAAVGGGLAVSELDSTLTFSLTATNFTENVAFSKAGALSLEYVYGSASMTGCRFESNWARYRGGAVLSSSTRVARASGCDFLTNTAALSTGGAWSAEKEGTITIEGGRVAGNSAKYGGGLSLNRDITLLASNVLFEGNTAQYAGALESLDCSLLNITTCSFTNNSAYAAGALSIFQASLGANILNCNFTANKASSNASANDACGRSGSGGGGAVCLDIAGSVRLLGGTFANNSATNGGAIWASQKCNPFTDDQCGYVLLLNTRLYENAAVGGGGGGVFVYESDDLNVSCSGGESMLLDAITFAAAAGSAANGTACNGSWYGNTALYGALIASTAMSMHVLTPLNSSMAEYRSNDPLVVTVELRDSFGQRISGGSPETTTVLNAVANSIQPLGVLTASASGGVANFTALRVRASPDKYTFNISAYGTIHDIPEAVVLVEVRRCVVGEVTNAEKDLCTPCVAGTYSYNPDNTSCDSCPDNARCSDTGTGGFVLLPEDGYWRSGPYSPQVISCPNSDACSYDGSTVTAAIPGITKARDVVLLDALMTISGGGTGIHVVMNNSQAAIGWYRALQCRKGYFGNVCGGCAEGYGQTREATCSKCPNRSLNALYYFLVSLINVLMIVITVRAQLVKGKEVRRKSQSVKDSEATATAPRLTNSTKDGTMDTVSGSRLPDAPEDGSCRGNPPEDGECGGTAKGDKAAEAAEGVSGDPKAAAAAKESPPPSGGPDSAQLTSSYEEDMRDDELVSGTPSIVLKILVSYLQVVSIFKDVALVWPSPVEWLLKLEMQVSFGITTLVSLDCSLPSTGVPKSVARVLVTMATPFIMVIMAVPVWVLLHPAHVLTRQKKGLSPLDRRRYVKVRVIMTVITIVFFTYPTVTNVLLGIFSCPLLDDLPPGGTPYGDQLKSVGRYWAKDYNLRCYHGVHRLLMLILGVPGVLLFSVGVPAFSAWFLWFNQKRLRRRNFFRAYGFLYSDYEDRCYMWESAIMLRKLVMVAVVVFLGAISVQVQLLVSLGIILVAVAAQIWYDPYRCPRMDALERISLYGTAILIYISLFFLLYLGDVMGLILTVLLLGLNGLILIWFVIVIIREIVRLIMRGLDTGEDGVVTAQDVMNVLEVMAIKRPWLAKRILKLDQLARKHPAVARFLSYAYPYGETTDPWIVAHLGLEPDFDPNPNPKPEPEPKDPGTDQTEPNDLSGKDRRQGSQDGFAEISGMAPEGARSQVQIRTVPSAQLGLEEAAAPTAAAAAAAAAAAATGAAAATGAASSGAVICPTVMASSGGNSSCFTRASYVVLVPSNAASVSRRTSTAALTSPVVTPTATRNAPMLLGAESATPEAAGGSVENASADTYTPSLPGMTQAGQMPTTPAVATPPAASVLSAPGSRPGSHTLSDGGRSTRLEGRTRSFLPPLQDQSPPAGLPPRNIATTDGSVGWQQLNPTQPPNATAERRINGTNAAMVGSRPGSATGESSGVWNGRRSLSAWEAKSPGERDWAAGREGVQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLX8</accession>
    <name>A0ABQ5RLX8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="547" mass="56076" checksum="A305200FE86DF122" modified="2025-10-08" version="1" fragment="single">PRSPASAYMYSTGAAIDKQQLSAAGSAESTHIRDPSARSASPRAPMPMRRLPRRVSLASDPYPEAARSPHNLRNYQHPQIAATDSGRLATRSPTPPPGGASGTGRQDHDRASPPTDPIESPLHAVLHSSVVAGMQRPRRARGATSRSSDGDGGSSASEPIAATQRHSFPGWDPGTAFLTRDHAVGAWAQDCAQEHLRQGPLQQQQQMDRVPTLTAPESYGRLVAAISEVSSEGRIVRGRPGAAGAGSTGSICSREEERTVVASSGQEDADVAAGGEPNQRDGDPAGFQEPSVRCAKSGGKVRAKPPASVVVPEGLVTSTLAAADEPVVEAVAVPEIWPDVDVGTEKPPLPPPPEQQQQEQEQPAAAVSLAAPGAVSSAPRRISDMHQEWTSAASGGTGAGAVHARARRGLASSRAGAAFTTKRLSLDMLPPGSTFTAAAAAAGTSAAAPAGPGTSTDINSPGPPTSVLYGSPLRHVSVSMKLATPPGAQYSDLEAALMATAHRNIQTVLSSGHSGRSGNVATGVGAVESRGHMPWGSQDNRGIAGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM10</accession>
    <name>A0ABQ5RM10_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_000374</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="9">
        <location>
            <begin position="70"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="10">
        <location>
            <begin position="223"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI58637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="496" mass="55424" checksum="44C2A2E9C282115C" modified="2025-10-08" version="1">MRSLKQILRLQQDAGALALRAFGTAAKDVVAADSNPFLRFSNPRPSPIDHTPLLSTLPETKITTLPNGLRVATESIPFAETTTLGIWINSGSRFETDANNGVAHFLEHILFKGTKKRTVKDLEVEVENMGGQLNAYTGREQTCYYAKVMAKDVGKAVDILSDILLNSNLDARAIDRERDVILREMEEVNKQSSELVFDHLHATAFQYSPLGRTILGPVENIKSITRDQLVDYMKTHYRGPRMVLAAAGAVNHDELVKLASDAFGAVPDEDPTTSVRALLAKEPYRFTGSYVHDRWPDATDCCMAVAFKGASWNDPDSIPLMVMQTILGAWDKNSTVGKHSSSMLVQAVASEGLADAFMAFNTNYHDAGLFGVYGVTDRDRCEDFAYAIMSHLTKMCFDVREADVVRAKNQLKASLMFFQDSTNHVAESIGRELLVYGRRIPKAEMFARIDAVDANTIRAVADRFIYDQDMAVASVGDVQFMPDYNWFRRRSYWLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM55</accession>
    <name>A0ABQ5RM55_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="117" mass="13024" checksum="41578D655F8B1410" modified="2025-10-08" version="1">MWGSGVGYNSCNLAFTYVRYWHVPLSYNLFHVGCRTELYLAMFYPRRRAPVCQERSVRRMGECLRTVRKECSGLDHALLSGSSGGSDSARHRGACVEVLKDEGHWSFAAGPGASKSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMC9</accession>
    <name>A0ABQ5RMC9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SF4 helicase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000492</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007694">
        <property type="entry name" value="DNA_helicase_DnaB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027032">
        <property type="entry name" value="Twinkle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12873">
        <property type="entry name" value="T7-LIKE MITOCHONDRIAL DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12873:SF0">
        <property type="entry name" value="TWINKLE MTDNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51199">
        <property type="entry name" value="SF4_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="123"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="SF4 helicase" evidence="4">
        <location>
            <begin position="1018"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="299"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="423"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="502"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1509"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1581"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="526"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="547"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="599"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="634"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="644"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1581"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1602"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1625"/>
            <end position="1635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1672" mass="177025" checksum="9FA1C46BEB457C6C" modified="2025-10-08" version="1">MRGYVGLCDVGSLHIRFCRPSPAMCGSASALAGVAVTPSGPRIVIALRSTPGTPMPVVPLPSASTALASPWSGAVPHHSRRPTASTSRTGLGVGNSSVRRSALLVVCRAVTVGDERQLSPVKYKCRYCGKAISRSTYKGHHTKTCIRRSEESCEQGRRVQESAEDLKRLRAAITKADDGIGGFASSDPDPGVWKDDPGASLRSAAQDVSVPTGRGDEDLGSSPSPSPSPGRPLGKVARVRSSPTMSPLPRAPQRNSAATLPNPPTSLPFTGSILLPGLPAAVPDFGKVPGAVPWAVAADPETKPQDMGNGGQGRQDDKSHNAGIAGEQNDWLAKASPAAAWDEAEGRGPGQPSTSSRASTNRFMVPQVEGAPAAQPPAAAKAAGLPAARLHQRLPVSNHVTSPSSTASAWPATYDPSVASLASASIRSPSSPPQPPAATTGAAGDSTRPSVRAMRGCVAVQQAVRGGPESSGMVSEQDAFSRSQDVAAAGGNGNVMMPGVFGSLPNGASQTSQGRGKRTGCGQPSIAPQQVQGTARGQMIERPRLQGAAGPAPGANLPSPQQQLHQLPTQPHASAAGPPGTWIGAASPNSAPLGPDVWGSSAPAASLGPAWEPSRVPSIPPPGRGSGISQLFPGSTSVNYSNGNGNGKSNGVGNSGEAVAVMASQMFLDDGQPATVRPRSDSVGPEVSSAAERQLAAILREGQQPAARKQFRKPKLEPLRWEGYDEEDPYFSSLLPAARDWLMGRRKIPEEVLRRNRVFCTLVPTGGGSPPRPAVAFPFFRSVNPESVAFVKYRLFDPEAGEPFARKEFRSSLHGEPIMYGTNDIVAGAYTDIIIVEGEMDKLALNAAGFWNVVSVPNGAPAATSASPSAPPEQVFVRPGERHQLRARVKQHYVFMDSFLATLPDLGRCRFTIATDNDPAGQALRRELLRRLGRERCWEVLSWDNENQLDAERGGMDSSSPGAAARSRGFRKDANDVLMLDGPSQLAVVLEGAQPAKVAGLTTFKEYEMEIAAYFQRQDPLQLGVSTGWSCLDPFYRVAPGELTIVTGVPNSGKSEWLDALMVNLAENHGWAFALCSMEKQPLPHLKALIEKRVRKPFRPVIRMTPSGPQMVEAMGLEEVVAGFNWLADHFHLIRYEEEDDTGGPTIDWVLQKARTAVLRFGIRGLLIDPYNELDSRRPRDTSETDFIREMLTKVRRFARDTECHVWFVAHPRQQKVLTGQAPGLYDISGSAHWFNKTDNGIVVHRRFEERTHPETGKRYRVALPEVDIKLQKVRNKDIGTQGEAYLLYDKATGRYEDPAMLGGATFAGGSGGVGSALQGGPGQLSGLDRVQRRNQELGYVFNRGSYSPQQQHQQPLQQPGADNIIDITLGPSPLPPVPPSQAAVIPAAASPALRPSSPSYIRPSQTEPQHQQMYQQMYQQTYAQPAGVVGPAAAPNRLNASHEPVASVTQPPVIAPPPAASPSETAVSEYSHGTAVAVEEVLLPRPGVSISESLSRVSQRGVLGALRTPATSGGAAMSSPQPTANSRAGKAKGGQAGSKEAQGRRQRAAVRPAAKETADLDGGFGDGVLAELLRNEQAAEARQVFSRDDGSAGGWQPDGSSSEDAEMDGEEEVTGGYAGGMLGARRRRGAKRSRLGAGGHVMVQRGGVCVEEEPERLGALESSLAENAGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMW3</accession>
    <name>A0ABQ5RMW3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000681</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.200">
        <property type="entry name" value="Cullin, PONY binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042460">
        <property type="entry name" value="DCN1-like_PONY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF31">
        <property type="entry name" value="DCN1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DCUN1" evidence="3">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="486" mass="52618" checksum="F04A79042FB2C36C" modified="2025-10-08" version="1">MATCDAMFLLLHEAFIAFDEVLTNEGRCWEACKAKVQQLQEQWHERGLSGMTAFNIITQYQAHIKWQDMSPDQFVKFYRFVFHACREFGKRNLQMNVAVAAWRLILPGRFRLLERWCSFAAASSVLVVTQDLWRQVLDFSRTVHEDLSNFDSAGSWAVLLDEFVEDMRTKRRRSYGGPDGGGCPERLKRRESSGTELSALFVGGAAGHCGFLTACDGDIGGVMGSSEFVGSMSPRCGSKRRGPDVDVVTEQLSAMPLGVGQQQQQQQYGTGTQILGCAIGRDPAVLLPVQACSQSRGAVQLNPTTHGYVQVASPYREAGDCAAYYRSSDADAVAKRQCVERQLQLHNPHSQQPTALPPTFSLGRLGPMGSVGEGVPLLCGGNAGTGTSGGRTASSGQSMICSTGSLDSGGSQAGSTGSATMDCWLQDHAAGAMKEHGLMQQHPHQQKQLQQSLCQTQPCQQRKAVKARRSGLSEIVKSAVSDALGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNA2</accession>
    <name>A0ABQ5RNA2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF60">
        <property type="entry name" value="SPERM-TAIL PG-RICH REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="41312" checksum="67DE9320245EA936" modified="2025-10-08" version="1">MAKEGNGQKGQLHKGPVTAPLKSVPGYKPVSSIPAKFETILYTGIREKKGFSVESRRFMEPDNDLPGPGSYNAQTAPLDRPRPESIGKKGLGPMASQSRRFSDRVFYTGPGPTDYRKPGVAIEDRSFSKAAVTSAFQEKATSSLVPLDRSSHLGPGAYEGSGVTRTGAKVDYLSKPGATSAFKNSTGHELILLGNLEAPASTSYDLGDPWAQTSSRGGGSVRGGTSSFGTGAGRGGASVGSMAATLDSLLFGAPSNAAGKPDTGPGPGSYELQDFSSIRAQLSRQANRPSPVFLTADSSPHKQQARQVLVSAGDKVAHDYLKPTHAPLHSLNPGKGISAPFRSRSAGHEEVIDQRAYSISSNAPGPAYYQPPTTIRKSFHKNAKQQYMTVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNC9</accession>
    <name>A0ABQ5RNC9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_000582</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45977:SF4">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45977">
        <property type="entry name" value="TARGET OF ERK KINASE MPK-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="314"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI58819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="363" mass="36813" checksum="BD34B56F42C160C4" modified="2025-10-08" version="1">MLRGFATARSTSMDQENGLGIAHGPNRRTSIQRRLDVGASHLSNQATAASSFGRAILFFCLALLVLGVLPAMFAFWACIIVLIFTCLRLRLQAQHVHQALELDDLGRRGASSLHLGTPGGPVLRVIPGAGLLLLHADPHAVLQRINVQLRHLDNIDEEMGVTESDLPFAHSPFSAATAGAGNFGARPPPVSDADIAALPCYTYKLPRGAAAASACGAAAVDASTTIFASPVAAAAGAAAAEGCAARSPIPQASSPASLQVASSSGAGGQPTPGLGRLSSGCAAAAAGATGGELVRARAIGGSDGGAVGPAGLTCPVCLDAVAEGSTVMTLPCLHQFHASCVTPWLRQQGIYATCPMCKTPVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNT0</accession>
    <name>A0ABQ5RNT0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="270"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="870"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1126"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1323"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1393"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1457"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1568"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1809"/>
            <end position="1894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1932"/>
            <end position="2045"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="627"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="775"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="207"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="401"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="882"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1137"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1171"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1197"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1348"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1397"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1485"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1569"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1617"/>
            <end position="1630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1816"/>
            <end position="1838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1875"/>
            <end position="1894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1980"/>
            <end position="1997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="2005"/>
            <end position="2015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2016"/>
            <end position="2025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2045" mass="209466" checksum="80D12C9891DE9290" modified="2025-10-08" version="1">MDEQEVASQSTEDASRPDSPEEQLQEPDVEPWTEGVAMDADDTLELLRDALSQGMDILESLSATERTVATAQAGLQTVSYDMHVKLGMELMALRRQEANMQLDAEQFGEQLKRRAFVRDMLKRVAMEDNWVQYPASDMERISRALDRSASKLQRADDQDGNGGKSAKGDKEDHPSYAKWLERRRKPNTAHRTAFGTTVSKKPPQKKATSGVSTVSSAAASGPRTSLSDAAGADANEVMTVTTFGLAPRLMGPVMSHRYLSLSVVKRYSTDTGNPVNPTGGSLDGGGTADAGAADSGDRNPDGDTISAAGGAPHGDAEGHHAVRFKAEDESMDGDGRQDLEDAAELLGLGGGATGGRDSRRGCRPQSRMLATSEGLGALEPTEKTGAMSDWRSVLRKKRAAAFTGKGHTTAAATDGAGGNGDATDEELAAANNPLQALRMSIKRELSLSAKLAAALLPAEEKPGTSTTAAVSESSTVASDGGTLTSILEVEAPLGVLEDAVRALESIGDEQLHAYMKAMEASSSPDGSGTVAVASVEVEAWAAGAAHRLAVAREEYSRAHTAKLETVTSISDLTQQVELLREWVASMYQKRLAAVAASLVVQRERLSAAATVSGGNRVSAVGKLLDDVDAAAEELDSMAAAVEAAASEAAAQLEERERRRAALRLIEDECVKLAEEMAAQEETDRLAQIMKAEAEAERLMAEAQNEDEARRHALVAAMLMGQEEWVLEKEARLAALRAAERDEKRLGVLRAVEEEKARAAEAAGRPYDSALLRELRELEEAARLEARMRRERLEEALAELAHFEEMLQRQHDGTVPELAVSSQEQTVVSPLPAGVTPEALVRAALEAVRECSDVAAAAAAAMEARAQGAVETGGDTAVEEAEASLAASSSAASQEVSGAEPADSREELLLHQASLAAQARLSAARSGLQAIVAAMGEARRVGSFGAALAAVPSSGEHTPDTTESAPETPTAVVASALASVSASMSSSIATTAASASPSASVSVSALATADIGQGVDAATLATPPPPPPPPPPRRAITQMASLAPRDAAALKAAQQDLEDEQARLAAILADADIKLGRRRSLLLQGLIDECSGLAVAVAAAAQAAAEAPEPSLAPSVAVSTAGEAMEAESLAEVETQTRGSPSRSAASRAAGEACGSPADAPAPAGLQGFEDASSAAAPAATEAAAAASPAAAEGALSPRPPSQSLMPPPQPKSMLQPPALSLQMASSLPPPTSPNGVLAPPMTRSRASGNGAAISTTTLTLGLSPRSDSQAPDWALRPDAQAAAATGRGGQNGGGISSVVLAGSGGMGPPPLPAVHPHVLGGAARAAGGSHMRTNTHHNPQAPHLHAHQQQQQQQQQQQQQQHHVNNQVDIAQPYSRTTPPPVQLQPQQVALPVVSPNGSQSDPNGSVSRRSLVAQSRPAAVVTPAGAPDFLPPIVSHVSNVVGAVLAAPPPTPRAITFDGTTAIGGLGNAHSPSVPLPASSRSAPQQQQQQPQQQHHQQELQPPHAHQQTLWAGAAIEPRSSRSLPASPEPSFKVVPGRVMGVTRGGGGNTAAAAAAAAATSRQAVTYNNSNNSNTNHDNSHSVSAPLMVSFPHSAGDDRRVGGMASPSSPLRGPSQHASGHTGRSSPPSVLTIGDMSYSQWVSSQQYMTARALEAAHQVDEVLARLPPELHSVLLNAAELLTLDKGGYEPVQMQPMAVGTAGGDGAGGVIFMPAHLQALPPVVSRYIFAQLQRIFQKANVGVNGGPGTVSAAGKLAAATAAAGSSASGTALGGEPSGLASTQSLAALELLNQLIPSIAQNIGEVRKGMNGMAGNHDGGSGGAGAPAVGPGGGGGGPASGPPVPSSAVSFDTSRCPIGTGAMHGSPLRSSRRTRGTPPDSGDGGSTSNSSASSSSVSTAAAAAVAGSSSPTVSGTSPPGARVRHHILSLSTQAHPPGAKAGGAGQRRSVTGFSVKRKGSTGDPRVQSSPEMERWTIRGVNVQQQSSKGAGPLQQQQHPDGPPALHGGGGVSGSRGVGTSAARGGVQQQRSAFLPPLSNKLATVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNU3</accession>
    <name>A0ABQ5RNU3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="398" mass="41795" checksum="54EBD6B578E89EFA" modified="2025-10-08" version="1" fragment="single">SNGSSTSTSNTRTSSSGCRVRSLTAAVSNPLRTSASFPIATPSLAAHDSPTMGVASTICDPSAYQSLSLLILLHECTRRAYGRPLPETDDLLSLPAQMDSLPCCVVLLVPEDTTQPLQHRAPSSPSAPASASPPNVAGHASIPTSASAAATYVAVGSSCSNNSSSHCSSSSSGTRSRAGELVRVQYLNRAATEALKVRANMTSENSAHWVEVAGLDVVAIRTMHRARGSPQRGEVRALKLTLTSSPASRQSGSHGGCGVCGSASAAAEERTIDCPEALLLPLMSPNGTCAGVAVMFEKWEVRGRTGRVELEGRPLVPEVPPDSAPGEVVVSQLQERVRAQADEVRKLKTQHGLTNKDPLVVAAVVTLQELKAELDLQERLNRAFSGSVSMLRPFQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP45</accession>
    <name>A0ABQ5RP45_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Dynein heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">VaNZ11_001037</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF1">
        <property type="entry name" value="DYNEINS HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0243">Dynein</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain linker" evidence="18">
        <location>
            <begin position="1284"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="19">
        <location>
            <begin position="1822"/>
            <end position="2157"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="23">
        <location>
            <begin position="2322"/>
            <end position="2475"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain 3 AAA+ lid" evidence="24">
        <location>
            <begin position="2694"/>
            <end position="2782"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="21">
        <location>
            <begin position="2858"/>
            <end position="3116"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="20">
        <location>
            <begin position="3130"/>
            <end position="3471"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="22">
        <location>
            <begin position="3502"/>
            <end position="3724"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="17">
        <location>
            <begin position="3970"/>
            <end position="4082"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="25">
        <location>
            <begin position="4118"/>
            <end position="4259"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="26">
        <location>
            <begin position="4294"/>
            <end position="4600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="184"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="291"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="883"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="4283"/>
            <end position="4314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3135"/>
            <end position="3207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3358"/>
            <end position="3420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3717"/>
            <end position="3744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF17857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="GLI59201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="29">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4604" mass="509011" checksum="38D2F7CEE7F50BE2" modified="2025-10-08" version="1">[encoded data, 4604 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPR1</accession>
    <name>A0ABQ5RPR1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001270</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051973">
        <property type="entry name" value="tRNA_Anticodon_Mtase-Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="278" mass="29340" checksum="0042209BDFB6F0F0" modified="2025-10-08" version="1" fragment="single">GDHEFGAQDQGSPIHLDRQHGDGRLRPSSCASSKEGAGTEGTLRRELEVSSRWLSTRVPRQGLRPHSLRTSKPKVSADRRYLALALLGVSSPPPIPQPHSPETKSEATTATALLSVASSDATTSVFTLDLSGDRAWHHVASLEGHRHPVLCMAHVPGVHSTHTSAGLAGDDCDVNASGGPGGAHGCNRRSCTVSISGSGCGMGEPRNTWCRSTPDDGYWLATGDTAGDVALWWVPRWLHASTGACGRGTDDEAPGRRVAPEPRVLRPVLSIRGVHQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ54</accession>
    <name>A0ABQ5RQ54_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="428" mass="48157" checksum="EC6200C5C8F1A333" modified="2025-10-08" version="1">MSSSDETLTKGDFLELISITLGAVFNPKLVRPTLERIMTELNIPDTGTVSETLLPSDSCDARALGGALPEQLGNGKVTTDNKEKGKRLTNYVVFVSWLSDFAAHYGRHPDIVKQKADKPDARMNQFYSAFYRTIPSAEKRALMKLLEGFRAEYSAINGADGSASILEALKKYEAAHPEAQIGKILGLEAKPEYMTDPAQMRAEAMEARRLAERKAAATEGTPMGLDVDKGDQQQEKRKAAAVSEPAGTSVQKKQKMVEAEGQQKQGQEKEKPKAEEKGEKEKAAKEKMTDKGKQKTKEEEERKDKGVKKAVEENKCSEKVAEEKKKDKAEKRKEKEAQKKAAEERRAAEEKKAAEEKKAAEERKVKDKKEKKKDEEEKVKDEEEKVKDEEEKVKDKGERKKDKEEKKRKKEAKESVAKKLKEKSEKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQD0</accession>
    <name>A0ABQ5RQD0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001682</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038760">
        <property type="entry name" value="PsbY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34790">
        <property type="entry name" value="PHOTOSYSTEM II CORE COMPLEX PROTEINS PSBY, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34790:SF1">
        <property type="entry name" value="PHOTOSYSTEM II CORE COMPLEX PROTEINS PSBY, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="450" mass="46136" checksum="E27D7D438A0F475D" modified="2025-10-08" version="1">MTECVSVCTPPSRCHLRLLDSCISRGKGTATMATLRMSVPLAVTMRANVRSAKPVLPMRKSVRALAVRAQANQEQAAAFKFGTVAAMTTVASSWMVAGHANAATELATLAASDNRLGIIATLFVPALGWVAFNILGSLQAQLDQMGAKNEPKRKRAVPAAIGLGAAASLLAAQSAEASTELATLAASDNRLGIIATLFVPALGWVAFNILGSLQAQLDQMGAKNEPKRKRAVPAAIGLGAAASLLAAQSAEASTELATLAASDNRIGIIATLFVPALGWVAFNILGSLQAQLDQMGAKNEPKRKRAVPAAIGLGAAASLLAAQSAEASTELATLAASDNRLGIIATLFVPALGWVAFNILGSLQAQLDQMGAKNEPKRKRAVPAAIGLGAAASLLAAQSAEASTELATLAASDNRLGIIATLFVPALGWVAFNILGSLQAQLDQMGAKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQN4</accession>
    <name>A0ABQ5RQN4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001851</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="122" mass="13503" checksum="04B12502D06A7E33" modified="2025-10-08" version="1" fragment="single">EFEKKPWKKLWNKESWAAWRSFKYAQPDNPDAGPSECRIVMEDLNVTDRQQELINALAQRKQIESSKTSFAVPATPVPVGAGGTAQHTPGLGALELPKGTMKSGRNDEPPGTQDGRTSKRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQU9</accession>
    <name>A0ABQ5RQU9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001522</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Tdpoz family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14736">
        <property type="entry name" value="BACK_AtBPM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18280">
        <property type="entry name" value="BTB_POZ_BPM_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034090">
        <property type="entry name" value="BPM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF187">
        <property type="entry name" value="OS07G0655300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="339"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="530"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="539"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="559" mass="58574" checksum="1B1A6FA76945E61F" modified="2025-10-08" version="1">MATANLVQRTASKTVTDTIVGEHTHTIVGYSLIKGIGDGEPIASERFVVGGHEWVLLFYPDGKRSSSEGPMGNGPAPDAGPHAPAVALPVAAAVAALPAGRDEALDAVGMPPPPGAAGPGPLGGPDARGGGGGGGPGAVADGVAVGVGVGHHAAALLAAQHHHPHHHHHPGHPAIIAQLGPMHRQQRRDTTNEYAALFVALIGEGPNPQGIVSTTEGKVVRAFHRFTLVDQTGQGRDLTKGRTRDAGAVKISCARQDPNARNCHGYRKFVKRSLLEDPTKGYLVNDTVVIRYTIELVVSSGGALMRGGPGGRGAAELVRVPPPCLGPDLAGLLQQGEGTDVTLVADGEEFKAHKFLLQARSKFFHSLLNNSMREGREGRAVVQDIRAPVFRALLHYVYTDMLPEGMEDGNLETEMAQHLLAAADQMQLERLRRICERRLCETVEVDTVAFTLALADRNHSEELKKVCLDFVGRNLAAVMKTEGYSHMVTACPGLQAEILSTVAALGGAADGSRSNGHGRGGGTVLAAVAVPGSGGGGGRGRELALDAEDVRRVRPRREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR66</accession>
    <name>A0ABQ5RR66_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001948</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF135">
        <property type="entry name" value="CSC1_OSCA1-LIKE 7TM REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="507" mass="57746" checksum="34F7E5C81441E235" modified="2025-10-08" version="1" fragment="single">QASYDAFCASSMASCIAHVGEVFPDAAINMTYGAEWSFPSATVRLLNERPIRQDLTKCAEGVGCSASYCMPCYCLGLSAVPTEEPTPEFLAHTEFACSRYWNVLDPRRYGILVAISLVIRVINSLLAYFLRLFKFHVERHWTITDTELSYAIWSYLVQLVNTVVVLLVVNCSSLRNLQKRALEQEPPWLQKLVLDGLFNDFTPEWYASIGVSIIILMMVNVAGPILRSLENWISKLIWQIWLLYGHHCRSSTKPPTMEVYQEAFGKSQFTLEERISDVLFNLTLALLFGSGMPLCYLIAAVYVLVAIWWDRLNLLAWRQPAQRYHPNLPLAIMYLLPVLLMGHCAFGLWMHTYFKAELSSTDIMAAARQGISSLSHSSLSRRITQPNGLPLLIWFLVLGAWLIFGRWIALGVYRVVWRLGGSCTCRLMEETLRPAERQDVSYEMALRTNSSADQLRGPRTYCISHLTNYKAFLSTGVAARLWSLVQGDRRFTRLYKFEARADARNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRR0</accession>
    <name>A0ABQ5RRR0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002363</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF12">
        <property type="entry name" value="AT HOOK TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046141871" description="WSC domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="3">
        <location>
            <begin position="556"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="891" mass="94572" checksum="1AD6B1D41C98A56D" modified="2025-10-08" version="1">MMKRLLLLIAILITYVAEVRCQQQSTRRVSGQLLYLTVSESAGQWLIVNNDSGLTPLRSGYTPPTIDVNGTRVFPGCAISLECFIDEATGVCNPNGLAPIFVTSRPPIAAVAPDTIISQNLLVIILDYSACGYPASLGEADVRSIYLGPNQDGKGGVAQKYAQCSYGKFTINVATFQAMLLQPRCTTPVTASCAPWAMAEIADAAAKAKIGIASFASFSHVTYVLPPRFTGHSGCPWAGLATVPGTQSWMQTNSYGVNRWATVMQEALHNYGLWHSWRNGVEYGDESSAMGRGMACPNAVEISRMGWAKPIIVNTAAELDGSMMDSGVLLSFDVPATYLKIAGSRNYVKVLPNWLIGFYDNPAMAKNLYISVRVAKNGDVDLGPEFASKVNVHEVNATVDNGYPHYLPNTDCKIQFLGSIGINSRLDLNSYKLVIFSGNWVTTDIMRVHICRYQTLASECRQPTPSSNPSLPSPPTLRHPSPTPPSLSPSPKSPPFPRPSSPPPRPPPPSSPTPPSPPNSPPPLKRSPPRPPPLRRPPPPSPPPPSSLPPPSTNAAGYPLGCFADDYTRKIPNSLESDLPMTPAICASLAKQAGYVVYGVQYGGQCFGGMDISKATSLGPSGNCNMDCTGDSSQKCGGAWANNVYTLTCPLVPGYAAVADMNHLGDDIKCDDGARMSAETAAIRCSSDTNCQAFNIYAAGVSSSSIGGSGCTKRASTPLKSSLGVCLYTKILTSCSLITGYTLTPGADHPGGDNICLTASSVTNAAKFCSSSSICKGFNLFNLSSKSLTFCSKNVAGPLSNAAGVCYYTKINTSCPQVPGYNLAADQEHIGDSLYCDSLSLTDAISRCTHDDECLGISAWWNVTNGALQACIKSATYPTTAKPGTCFYSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRZ9</accession>
    <name>A0ABQ5RRZ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bystin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bystin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007955">
        <property type="entry name" value="Bystin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821:SF0">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05291">
        <property type="entry name" value="Bystin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="56821" checksum="AC6F93DF0D207B8D" modified="2025-10-08" version="1">MPKKARIRKPKHKQSLQDEIEDPDANGVRTKPRAEKKRRRPDEEEEELLDAKTSGRILKAAREQQQELDAEELFDLDHDDDDEDGDGKKRVVDPQAALRAALQRLGTGGQSGKGAREDDDSDDDSDGRGAPFLNEEEQYEEEEVEVDPEDQRALEAFFAPGADSYRQTSLADLILSKLRERQAEQGLPQVPQEGEDDPGSGAGPVPEGLEPRVVEVYRGVGKLMSRYTTGKIPKAFKIIPSLRNWEDVLYMTDPDSWSVHAMYQATRLFVSNLNARLAQRFLALVLLPRVRAEIRQHKRLHFTLFQALRKATYKPGAFYKGILLPLCQSRTCTLREAVILTSVLKRASLPVLHSSAALLRLAQLEYCGTTSFFMRVLVDKKYALPYRVIDALVDHFVRFADDERQMPVVWHQTLLCFVQRYKHEIRAADLQALRRLCNKQHHYKVSPEVIRELDHAMGRGATAAAGTQGGGVAPMAVQGPASGAAKVGANVVENVRDMPPVLMMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS34</accession>
    <name>A0ABQ5RS34_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053234">
        <property type="entry name" value="RPM1_Interactor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33443:SF30">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-2C PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33443">
        <property type="entry name" value="ZGC:112980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="646"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="783" mass="85154" checksum="A40FB187C0EFAB75" modified="2025-10-08" version="1">MDAVAYRTRARCKETLPEPARRRYCSKPHQGKPSEIQGRRRLRKAVASDSQHEELNNSLLGLEGCNDQDDSVASNEARAPDMDSQKEAGSARKRKRCVLSSPTTSSADSKARPSFDDSDVIIVGEYQSNDRDVVVSEPTGGAGLARASCSVNPKPVTAAAATQVSGDDLGEDEELRIVGMVGQVVTRDLPHPRPDCAEHRFVQSSSVAESNSRCCSKCYCYVCDVKASECQFWGSGRRERDHANAWAHPAWNALRQAMRNGDKARVQQEFAEDGPLRTPAMSPKASILQQLMVRQMAEFRRHTQSWHERMNHQRQQQNERPMDAPAPHAASASCDSGEDVLDLDLSEVDTFLAALSQIGFSGANGEYTSSRPAGEAIARGPPAGVPETGRRQRQRQPAATMMAAAWAGAEAAGQQPAFGGMTARGASTMAIASAPTPATAATAASGVPPRSGLQPQLQLNELGPNGSVTWNQTAIPASNLPGSGQTTVFQAPNTRGAGAGGEGGAQPTAPCQSQDGRVQQASPHLHGPLQPQQQLLQQQIQQQQQIQQQQQIQQQQQIQQQQQIQQQQQIQQQQQIQQQQQIQQQQQIQQQQQIQQQQQIQQQQQIQQQQQIQQQQQIQQQQSTGMAPQPIGVSSHTHISVLPPQHQNAQQQLPSQQQLQPMNPQQPQQRPYHPCQPYHPYQAWVQPSPVVGYGGGCGQQPSSAQRQAPMSWAFPAAAVGPQAVAVPPPPLPLAPMVAVGAPQPNWSPISGSFIHTSHGPMVFYPFPPPPAYTHTSAGVQKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS90</accession>
    <name>A0ABQ5RS90_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Uridine 5'-monophosphate synthase</fullName>
            <ecNumber evidence="5">2.4.2.10</ecNumber>
            <ecNumber evidence="6">4.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_002600</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the OMP decarboxylase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="5"/>
    <dbReference type="EC" id="4.1.1.23" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010382">
        <property type="entry name" value="PRK13809.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF9">
        <property type="entry name" value="URIDINE 5'-MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="14">
        <location>
            <begin position="322"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI60445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="552" mass="59382" checksum="5197988C774810F1" modified="2025-10-08" version="1" fragment="single">SEPPTVPWSRKFRHRNIMVLGLNTIAEFVSKTFRRGTATKLQASSREPLTQDEIEDLVLKLNKIEAVKFGEFKLKSGLMSPVYVDLRVIVSYPDVLEQVSRMMWNAVRGCQYDIICGVPYTALPIATCISLGFGEAMVMRRKEVKDYGTKKAIEGAYKAGQRCLIVEDLVTSGASVLETLEPLKAEGLLVSDVVVLIDREQGGEAHLAQHGLKLHAAFKLSAMLEVLTKHDLVSGEVANKVRTFIAENQTSLPSAPPATATATATATTPGDVLPVAEATGPVPVSGKVPKTMERLPYETRIPLAKNAMAKRCLEVMVRKQTNLCVAADVDTVEEMLELADKVGPYICVFKTHVDIFDKWDNNIAATLTELANKHDFLIFEDRKFADIGNTVVSQYGGGIYKIADWSHITNAHLLPGPGIIDGLKAVGLPKDRGLLLLAEMSSKGTLAAGAYTEAVAEAAEQHSDFVMGFISINPAAWNGGPGSPGLLHMTPGVQLAAGGDALGQQYNTPMNAVAVRGSDVIIVGRGVIMAADPAAAAKQYRDAGWAAYKTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS93</accession>
    <name>A0ABQ5RS93_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_002255</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="8">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Plastid</location>
            <location evidence="8">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the 2Fe2S plant-type ferredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010241">
        <property type="entry name" value="Fd_pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02008">
        <property type="entry name" value="fdx_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43112">
        <property type="entry name" value="FERREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43112:SF9">
        <property type="entry name" value="FERREDOXIN C 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="8" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="9">
        <location>
            <begin position="101"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="201" mass="21838" checksum="35E749396626267F" modified="2025-10-08" version="1">MPPSAAVRMQDGVQNAFLAYRSFLFCVRVSSPSQNKPSLCWLSQIFLVVTTFLSFFTTGKKMCSRAITSSCNIRMSRSAAFGSQHPLRSTRILPRAARTTYKIEIEHDGKKIELAVPEGESILSVALDKGIDLPHDCKLGVCMTCPAKLVSGKVDQSGSMLSDDVAEKGYTLLCVATPKSDCKILTISEDELLDLQLVAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS99</accession>
    <name>A0ABQ5RS99_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002602</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050680">
        <property type="entry name" value="YpeA/RimI_acetyltransf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43420">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="65"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="245" mass="26660" checksum="0CD0787A617BD1FA" modified="2025-10-08" version="1">MLPSYVGTARPHNPLYFCTARGYLRQLTRVYNREADTRPSDRVHHIAVLESIGDFSWNNVKQVSALLSAAFYEDEDAWNRGSANTREPPLAETLFGWASLFAVLTQHLLCGNKYLTFLASENGKAIGCCGLSFDPAPEDVIAATGVAAGSQYGLLTGLAVAHAHRRRGVASALLEAAAAAAVARLPPPAMLALLVARTNTSALRLYTRQGFEEQPDWVDARWQAEAESGKVGKPRRLLLVRRLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSE9</accession>
    <name>A0ABQ5RSE9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_002675</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04337">
        <property type="entry name" value="Rieske_RO_Alpha_Cao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.380.10">
        <property type="entry name" value="Naphthalene 1,2-dioxygenase Alpha Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015881">
        <property type="entry name" value="ARHD_Rieske_2Fe_2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050584">
        <property type="entry name" value="Cholesterol_7-desaturase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013626">
        <property type="entry name" value="PaO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266:SF19">
        <property type="entry name" value="CHLOROPHYLLIDE A OXYGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266">
        <property type="entry name" value="IRON-SULFUR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08417">
        <property type="entry name" value="PaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00570">
        <property type="entry name" value="RING_HYDROXYL_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Rieske" evidence="12">
        <location>
            <begin position="312"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="139"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI60511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="654" mass="73115" checksum="EA1AEFB0712EAB82" modified="2025-10-08" version="1">MFPTRHQSGVLCKHGSGVIGMKVFRAAIKAHASAPQQPPADSIKQQATPSIAYEDPEAKFRRYGKNFGGIHKLNLDWLEAVPRVRIRTKDSRQLDELLELAVLNERLAGRLEPWQARQKLEYLRKRRKNWERIFEYVTKQDAAATLAMIEEANRKVEEALTEETRERTAVGELREQLQNLQLQVQEAQERLKLTQARVEQNLNRVNELKAEAVTLERFRSSLSGANNATAQQEKIQAAASRGVAAAAPTQAVVVREADAGKTVHAMLTETTPTNSSAAQAVGAPGRRSKAAMRRGRGLESSLEFEAGLRNFWYPAEFSAKLGKDTLVPFELFGEPWVMFRDEKGQPACIKDECAHRACPLSLGKVVEGQVVCAYHGWEFNGDGHCTKMPSTPHCRNVGVSALPCAEKDGFIWVWPGDGLPAQTLPDFARPPEGFQVHAEIMVDVPVEHGLLMENLLDLAHAPFTHTTTFARGWPVPDFVKFHTNKMLSGYWDPYPIDMAFQPPCMVLSMIGLAQPGKIMRGVTASQCKNHLHQLHVCMPSKKGHTRLLYRMSLDFMPWVRYVPFIDRVWKQVAGQVLGEDLVLVLGQQDRLLRGGNTWSNPAPYDKLAVRYRRWRNSVSPNVAGPDGPAPTNPVAMSAGQMFSIDEDVNEPLMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTD0</accession>
    <name>A0ABQ5RTD0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002655</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="82"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="216"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="337" mass="35708" checksum="DF1CD58D9B8E8D59" modified="2025-10-08" version="1" fragment="single">FPPPRPPPSPPSLPPPPRLPPSPRAPTPSPPPPRPSRTVLPFASSRPSLSPSPFLPPPTPTPPLPPQPPPVDDDRCEYIGDVRLVGGQSRSSGMLQYCGDDGSGIPKWGSVCGGGFKVSEARAVCRQLGYKDGFPILTSYKPTYGANGQYYTFLPNSPLMPIMLNGLQCPTDAPDLSFCNAKTTPPCTHDDDVAVVCLNYLRPSARRYICNNLGGVRLVAGRTPAEGTLQICRAGVWGTVCSDGWDDADATVVCRQLGYVQGWAVGPTGRTLMDATIGPFPQGPPDMRVWLSQVDCRGTDTNLDSCPNGGHVAVKLSCLDHSRDAGAFCSNVTSSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTG8</accession>
    <name>A0ABQ5RTG8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002685</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="28725" checksum="CAD7730636342B00" modified="2025-10-08" version="1">MNMQPSFTPEEQELIDRLREKYGGLSNGQPNPTDKVDASCVNCSAGAQSARQAEPSSRSSRATSNVEASICSHCHGTGTLVEIYNHRRLENFCKECDGRGVRVFKNGVEVQPGAATVGPLTSSGAAAPAGVVALHRRSKEASGQAANSLDRPSSDDEESVAEKAAALQKDLQRITVKVASHTKERLDTLAILQQPHSSWIADPEGREQAARDLVAQLNLQLERLELMRRKKQLALDRLTAAAASQASEAVNINLRDIISNEGVRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTM9</accession>
    <name>A0ABQ5RTM9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Ubiquitin-fold modifier-conjugating enzyme 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003177</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ubiquitin-conjugating enzyme family. UFC1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11686">
        <property type="entry name" value="UBCc_UFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014806">
        <property type="entry name" value="Ufc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12921">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER-CONJUGATING ENZYME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12921:SF0">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER-CONJUGATING ENZYME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08694">
        <property type="entry name" value="UFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008716">
        <property type="entry name" value="DUF1782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR008716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="166" mass="19170" checksum="0EDE1B692E21EEE2" modified="2025-10-08" version="1">MAESWDPKTRDTVKKIPFLTETAGPRDKQKWQDRLKQELHALITYIKMNKESDMDWFTIQPNSDGTNWKGKCWYVHELIKYEFDFQFDIPATYPDTAPEIEIPELDGKTAKMYRGGKICLTIHFKPLWSKNSPHFGVAHALCLGLAPWLAAEVPFMVESGVIKPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUG0</accession>
    <name>A0ABQ5RUG0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA mismatch repair protein S5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002852</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032189">
        <property type="entry name" value="Mlh1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF12">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16413">
        <property type="entry name" value="Mlh1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="7">
        <location>
            <begin position="215"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="398"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="566"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="836"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="401"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="567"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="953" mass="102472" checksum="05E9BCE010B6E163" modified="2025-10-08" version="1">MIPPVIRKLDENVVNKIAAGEVIQRPASALKEMLENSLDAGSTQISVLVKEGGNKLLQIVDNGSGIRKEDLAILCHRHTTSKLREYADLETISTLGFRGEALCSISFVSHMTVTTMARGAQYGYRVTYKDSEMEAPGPRPVAAVPGTTITVEDLFYNVPTRRKALKSANEEYGLILDVVGRYAVYSTGVAFSCRRQGDVRPDISTTAAGSRVDAVRSVYGADVARELIPLKVAVGGGTGPEVPVDGPMGLRIEGLISGANYSTGKKTVLVLFINGRCVECSPLRRALEGLYGALLPKASRPWIFLELRLPPRQVEVNMHPTKREVGFMHQAEVIEEIRQAVEAKLLASNESRTFAASATLRTQLAFASLPLAQRQSMPEAGRSNVADDDGIAVVAGNGREAEDDGDQDGDEEGEEEREDKFSRRKEQWKSTTAAAPIRAPGSSEMERKRQASGAVPLPYRPEKLVRVDYRAGTLDAFVIPKDAKDVRSTSVENAARAAATAGNVARKRSTAVRLAGVGPLDGVVEEGSSEQRQSRAAHTLPPGLITAEVLGVRPYAGVFDNLQAQGEARDKGRSGDADADTRVPQRRGPPPLVTADPAVLELWAEAEGGAHAGLGELLREHTFVGVADGSLVLLQHSTRLYLMDVGTLSRDMFYQLALRRWEQPLSLSLEPPPLVSELVALGLQLLKVKGEWQPEDGSPEELGELVTELLQQNGPALEKQLGLVVDLQVALAKDVDWDHPRERVRSIATALGDLYAARPPLLPDCEPKRAIDNIGHTELAGNRSASTVGDCLEAAGDDRRYPAGSNRNGRLELGAVGQASKDGLQGGVDCKSDVKEKAVYSPSGPSGSGGVGTHEGTDLLKISERDSGRQPTGNRVDAMVEADTSAAALEPSANANELKDERGRALAAYEYKLKQVILPAMRGCLKPPCARATDGSCVQVAALERLYRIFERC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUS6</accession>
    <name>A0ABQ5RUS6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045005">
        <property type="entry name" value="BPM1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379">
        <property type="entry name" value="BTB/POZ AND MATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26379:SF187">
        <property type="entry name" value="OS07G0655300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="2">
        <location>
            <begin position="70"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="350" mass="37466" checksum="1A3E90EF9A720056" modified="2025-10-08" version="1">MPGDSAHSSDGYHLTNRKQRRSRYHASNHWGPVECSTSDSEVEAPPHVHSYTGLAGCLETGSVRHVDTLCGTHTYAVVGYSLARAMGPGTRLCSDVFEVGGQLFRLEVYPAGLNTDTNKYVSLFLTTPGTTGLRYLLYELSILDKGPSQPQHITESCSAHTPSQTGRAAIVAPQIGVVAGFPKFIKANSLHRRARRFLPSDTLTIRATVKVLTGRSSFPVAIPGQTQLFSLQPNLGWGMPSVLAQQRSPFCVPSSLYSPAAAGAPAAPIAVGTQPLQPYTERIACRCGSQTQQPQAQVLYTPHLGGRQTEHLSLVPFPAATPPLAMALPQQAQFAAIPLSATFGHPGMMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV00</accession>
    <name>A0ABQ5RV00_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pyruvate, phosphate dikinase</fullName>
            <ecNumber evidence="3">2.7.9.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_003409</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>pyruvate + phosphate + ATP = phosphoenolpyruvate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10756"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.1" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.10">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.189.10">
        <property type="entry name" value="Pyruvate Phosphate Dikinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010121">
        <property type="entry name" value="Pyruvate_phosphate_dikinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004531">
        <property type="entry name" value="PRK05878.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01828">
        <property type="entry name" value="pyru_phos_dikin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE DIKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931:SF9">
        <property type="entry name" value="PYRUVATE, PHOSPHATE DIKINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000853">
        <property type="entry name" value="PPDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="13">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="13">
        <location>
            <begin position="148"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="13">
        <location>
            <begin position="386"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="12">
        <location>
            <begin position="507"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme C-terminal" evidence="14">
        <location>
            <begin position="601"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI61134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="966" mass="104859" checksum="81CB8DA421FC72F2" modified="2025-10-08" version="1">MQKHVLRSLPGSGSIPTGAGKVVAPPLRAQGRTCRHTAAARIADQVQGALPVGPLAKHRHLKHNASRIVPSAATVDSSTVVKTKRIYTFGKSLSQGDASMKQLLGGKGANLAEMSRIGLSVPPGLTITTDTCADFHINGRKLPDGCWEEVLQGLRTVESEMGCNLGDAKAPLLLSVRSGAAVSMPGMMDTVLNLGLNDACAAGLAARAGDERFAYDAYRRFLDMYGDVVMGIPHHLFEDQLERLKEERRVTEDTALTAADLKVLVGRYKEVYKQLGKVFPEDPYEQLRLAIYAVFDSWQSERANVYRSVNGITGLTGTAVNVQAMAFGNMGLTSGTGVCFTRNPSTGERKLYGEYLINAQGEDVVAGIRTPEPIETLARTLPGAYQQLLDNCALLEKHYKDMQDIEFTVQEGKLFMLQCRSGKRTGTAAVRIAVELVDEGLVDPDTAIMMVEPGHLDQLLHPQFADEDAYKKDVVGKGLPASPGAAVGQAVFTAEDAEAWRSSGLPVVLVRRETSPEDVKGMYSAEGVLCQLGGMTSHAAVVARGWGKPCITGCPDLQVDEHNKLARINGAEIRQGDFISLNGSTGEIIRGKQPLAPPELAGDLNRFMKWVDERRRLGVLTNADTPEDAEEARKFGAEGIGLCRTEHMFFASEERIATVRRMIVAQTQEARLKALSDLLPFQREDFGGILRAMSGFPVTIRLLDPPLHEFLPDGEMTEVCEALARDAGITIDEVMGTIEKLQEVNPMLGFRGCRLGITYPEITEMQVRAMFEAAVGVLRDGGSVQLDIMVPLVGTAAELKNQEQLIRRIGEEVVKQSGVRVPYRVGTMIEVPRAALQAGAIAKTAEFFSFGTNDLTQMTFGYSRDDIGKFLQNYLDKGILQYDPFQVLDQDGVGELIKFAVERGRASRPNLKIGICGEQGGEPKTVAFLHEVGLDYVSCSPFRVPIARLAAAQAAVRSAKAAAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVB3</accession>
    <name>A0ABQ5RVB3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003445</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045120169" description="Protein kinase domain-containing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1577"/>
            <end position="1848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="987"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1156"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1240"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1489"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="993"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1240"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1259"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="1609"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1869" mass="193084" checksum="2499BE5492230406" modified="2025-10-08" version="1" fragment="single">MSRVVTLLLLALLLYHCYGQTSSLLLQSQLFSLSLSLQQESAEDAAKRCVNSTELNGPGVLAPATLLLYVLQDIISKNTPQLQAAVKQDPVQYNTGGSSLWVMDPWGSCRAIILPARLSSYTGAVELSLVPCSWTLQFMCMQYSVAQALSAGLTLPHNLYRTPTPPPLPVAVPSYGGNAFDVVPAGYRLVRSGVTLGYSSLPAGVVFFSGRQLYQQLGPVVNIVGRWRATGLDALRVVNANDATNVVEQAMANGTVADYFGGTMLATPLTTAWAATPLPRENADTGWLRTSYITRLEGCSSSTAVQRLVLVNAGGAAAAQGVTRCISNFAEEVAPGGFLAFLEGYFVDYTNATLEFASAPSIVQLWFVWAVPEKYADEDDGGVASYPIAPPQETLATALMSSSPPQYGKGGVRAIAPRLTPPEPTAQCTQGRRMIPPYSPIRNRAECGQSSLEQFACPSYMCCGTTRGRVGMCGMVASACLLSACDPRYGLCGVIPNEALEVTPHEGLMGVAPAFSPLSSAPPYTTDFGGDGSSMATATVRFGTSVYVLDRSASVTYNAALEACRSQALYGLSWRLIMLPDAFSYEVTLLGRSTFLSLLDNRVAWVQPRPDNSTLGVAGCLTVTWDVDMSSVQLVAVQATECSSIASVICKATWPAASMSKHGTVRTQQAPVTVVRWTPGVHSLSTSLVGVGQPNSGPMCAFESFGGWEHSQVVQAMAQLNALQLNSSSGAAPLMPLLVNVTLDSPLAGIAMRPLVTWSSIASESPAAYEAVGSIAVQYLTGSGSDAALAPPIILGSLDGTYLSDNASYGAFAPAAPSGVASYLSSSTGWESLQLNMSDGEVVVEVLGCRGALLEHMLLRTSTGRLWTAPMGAALTCSVPFSRRPPPGGYLVALQVFGGSFVEEVWLIWGTPSNPPAPLSPAAIGPATGAVTATPQVKASSPAKSQRTVVFGIGVSFAIVLPVLAGAVLAWWVCRIRYRQQHRAPVAAASLPQRRQSNSGVSFPSGQGGSISSERDGRDSAGPNCRGLLGILTKGARQGRLLWLQVCKGNLGADGANAFARLGQQAEVPLSSSAGGEVGVSPWSNATILSSTCKAHVALPRKSGGMPPLYAVADMTLTRAEAMAATATAAAEMAAMATPAAAPGAAAVADGYGNAEGTRRKGSELSTGAVGPDGEVQIGFRPPSGSVATAPASSSDAQHSLLCGTTIGTGTVRDSGASGNIDSCSQRACAADIIAGPGPGSSSASFSVASQQIGGRGGTEAAAGATWASPLQQPPPPPQQQQQSRTLTTLASPGQPVPPPGSEAWAQSLHAALFYKVLYHWKPPSLATPLVVSGAGPGTGVVALPTSPPWPPPAAVVPVAAAPAAGDSYWVGTAVTAADTAPPLSTISIVPSSSIADPLTSSSGGGGGSGNSGVTHRSTEQLAAAAAATQAPLAQLLAAAAASYIQAAWGAANGVMSPAMLPQPLGGTATGSTNVSSAHDGDMLAADDAATTAAPPPSGSGVGPYSRSQQSEASAAAAATIASVPVVAAHAGAGAVAAGTIGGCGGSGIATPSQAAPPGEYDGTELLLGRDVRVDVDDPSSYLGHGTSGVVRRGLLLQPDGSWRPVAVKLLNQPNDQAVDAYRRHQKTLLQEITILGSLRHPNIVQLLGGSVSLQPGASFLVEELCGRTLSHAIYDASTPYSLRQVLLWSCDIARGLAYLHPNIMHRDLKPSNVLLDQYGTAKISDFGLARFKIHTTLVTRDAEVGTTCYMSPECFVSNDFKVTAACDVYSLGVMMNEIVTRQPPWSGVRTAVVGFKVAVVGDRPRLPAEDCPLCPMGLRDLIRACWAQHPEERPSSAEVLRRLQALLAEVEAAEEAGLEEALKGKEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVQ2</accession>
    <name>A0ABQ5RVQ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF31">
        <property type="entry name" value="OS01G0873500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="16980" checksum="FF141A23D128C330" modified="2025-10-08" version="1" fragment="single">MGKDKLAEPLDLNNYSTWAYRFENLCAEHNLNRALLTEPVTDEEVAASERVKTLLVKNVKDCHLPVIKASPNAKMAWDGLAAIFAANNHARRSQLCQEYTKLAMKKGEALSVYLARVRELHTDLLKVGQATSEQDMTYAMLTGLPQKYEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVY3</accession>
    <name>A0ABQ5RVY3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_003507</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="7">
        <location>
            <begin position="399"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="125"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="645"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="647"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="675"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="882" mass="98189" checksum="F151F0ABA65CFD0C" modified="2025-10-08" version="1">MKIEPEDALLHLIRGLSSDYDGVKSVLRHMESIESVDQLQDMLKTEEGELRLQRNKAREVSLMSLSRTHDGARVGPSQGAGAGNADGGRSRKACLYCNKEGHFIAECRKKANDEQRGIWRPALKGLRQKPRNPPALAAPPPAPPPVAPVAQPPAAAQAAAPPRPAQGQRRQLIARVYSSQQDDWERELWLFDTGAEVHVTGDKDKLRNYQHCGSDCKVWLKTANGSVVRPEGHGRVMFRSSCFETDVEVDVYHAPGIMGHILSLKALEGSFGPNVVPQFSGVLPHVEIGGAPAIELEKIDGLFYAEDSSYDSWMGRYGSDRCLSGKKVREELRKVKLAITKQDSNLGVLWHKRYGHLGFDALAKVASSDMVTGFGASVDDVVAAKGQFCKPCAEGKQTRQPHPSTGNVTSKRLDLVHMDLCGPLPVKSWNNAKYWVTFYDDCTKFSEVRCITDKTVVPETVKEVIKLWERQTGDKVRRIHTDRGLEYVNEELASTLRDMGIFHEKTAPYTPQQNGAAERLNRTLEECVRTMLADSGLPQEMWSEAVITANTLRNVSPTKGSNLTPWERFIGEKPDVSKLRVFGCPAYVMIPKQQRHKLDPVSVPGQFVGYAPGMSAWRVWIPKDRRVRISCDVKFDEWGVRGSMEFDASESEEEDVSGSDPEADEGNEIQEESPPEQPPEPAPQPEPELDVPIALRKSQRQNTKYAKSILGVNVDIPATYEEAIASPLVDFWIEAMEEEFASLQEKGTWSLVEKPADKQALGVKWVYSVKLDKHGVVERFKARLVVKGFMQREGVDFEEVFAPVSKHSTLRVFLAMVAADDMELHQLDVKTAFLNGDLEEEIYTVQPQALRVVVQASSVVCTKRCTACARPRAPGTRPCMRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW16</accession>
    <name>A0ABQ5RW16_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003508</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="4">
        <location>
            <begin position="2"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="4">
        <location>
            <begin position="107"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="226" mass="23945" checksum="899E56A74309A5C7" modified="2025-10-08" version="1" fragment="single">GFAAAMDALGITNDTTVVLYDHLGIFSAPRAWWTFKVFGHDKVAVLQGGLPAWRAKGLPVETDNSPADEVMFAGSRACASPPAETQYRAVLDRSKVRTLEDMVANIKTRAEQVMDARSGGRFVGTEPEPRPGLRGGHIPGARSLPFPTLLESGAYKPPGQLEAAFRSAGLDPEGLIVGSCGSGLTACILALGLYQVNGKLAAVYDGSWMEYGARDDVPVSTLPEDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW58</accession>
    <name>A0ABQ5RW58_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="41625" checksum="AD4024F666BAFDC6" modified="2025-10-08" version="1" fragment="single">METHSPVVCKLLVCCPNEEKRRIDTEINLSVVDFEQLLTDAAGAKSISEVSVQVLDSEDEDGKLVAQQVAPVQLLSRCTRWLKKEISAPFKGKAWNQAQKKLTIHVSLKQPSVQLVSPEAAINKAVRDNVPYNLDSSLKNPRVLIAKDIQREPHLQPVWDKCFAAGFAQVADLQPRVTVDCFKKKWQKYATDYNAQRAREEKVKATHKEELACKPEDLEKKPWKKLWNKESRAAWRSFKYAQPDNPDAGPSERRIAMEDLNVIDRQQELINALVQRKQIESSKTSFTVPATPVPVGAGGTAQHTPGLGALELPKGTTKSGQNDEPPGTQDGRTSQRQKQPAHGEDALMMVLAANAARIHQKEKEIKAARRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWB1</accession>
    <name>A0ABQ5RWB1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-deoxy-D-manno-octulosonic-acid transferase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004329</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11720">
        <property type="entry name" value="3-Deoxy-D-manno-octulosonic-acid transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007507">
        <property type="entry name" value="Glycos_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038107">
        <property type="entry name" value="Glycos_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039901">
        <property type="entry name" value="Kdotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42755:SF1">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC ACID TRANSFERASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42755">
        <property type="entry name" value="3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04413">
        <property type="entry name" value="Glycos_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="3-deoxy-D-manno-octulosonic-acid transferase N-terminal" evidence="3">
        <location>
            <begin position="39"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="730"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1161"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1288"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1748"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1803"/>
            <end position="1847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2176"/>
            <end position="2211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2275"/>
            <end position="2333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2476"/>
            <end position="2571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2661"/>
            <end position="2693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2745"/>
            <end position="2822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3147"/>
            <end position="3184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3196"/>
            <end position="3224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="731"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="748"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1165"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1308"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1815"/>
            <end position="1837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2530"/>
            <end position="2553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2682"/>
            <end position="2693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2752"/>
            <end position="2761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2796"/>
            <end position="2805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2811"/>
            <end position="2820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3171"/>
            <end position="3181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="3197"/>
            <end position="3208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3329" mass="346201" checksum="69998E38913B847D" modified="2025-10-08" version="1">MKRNNFLAYIRGVAKLPGSLAYLALRLLHNQETIGTALQRLGWSSIERRNGPVLWFNVNHLGETSTFLPVVFRCLQEYNEKVTILVTTPCLDVYKLLGGSLPHRVLVQLVPLENPITIAAFLRKWQPQVGVFLETPFHREFAFLARDSGVRLALLNAHMPGEDMLEWHARHTSREVLKQTVSLYSLIVPRTYIDVGRFRMFGATLGQMPGWSTDLQQASELGACAARLLTQRPRIHRPLLKRLAGRQLWIATNTLPGEDVVVARIHAALRSRHTGLLTIVSPSMPKTSNARVLAESSCSSYAAAVRRLSLERRLQVELLSTAIDDPKRLSADVDMLVLDDPGTLAAFYSLSEIVLVGGSLLPSLDGASSPAAAAVAGCALLMGPHGGEYLGLALDLNHAAIVASEEVAAAVAQTGVTTGRIHQGDFNTPREAEGRVSCTPGTPVISPWLSGPGVGPPSGIACAMGSVDAPGAYGRNRPWLTHGATSTIRPMSAPSGPEHFDLRSYNMPYAFLPIGGASDVSSLQASSNGQRNIFIAPFSTPGVSYSWGGTGSPAVTDVSGRPICSDDVAPPSARLAGFRGGITKRRRGALDRFRWDFDRDIAGFGYIPEKDYAVSDSASSTCGSEEDHLGRGVGESALLVESVAPTVSDIPNAEGDAGGTMLAANQQATAATHNVCSIAPSSLGQVVFLGEQTAQKAPGSPRPAQDILSVEPVTVVLPTHTMGLVVDSTGSFNSSADSLSSPSRASLTDRSNPAARSTTACDEAVASDRDDGDFHDAIDVSDVDVDPGLLVSSLSHSGVIHSNANTPRSDPGDKLPATSIVTPLLATPEHSNNAWDEDVAVDVANALNIGAEVVWTTEAMEVVPGEAAVEDRPLLQTVLRSWKQLVATTGCGVPGAKGITINIPAGNGSSEESILPSSTELPVLRGTHLGQSILCREPDISDDVEVHWPSRYAVCEARHLASDPGLPSIPVPGLGDEGGDFFQEWPAPSVKSVIDPAQLSLAQAVHANNTLVPQQLSHGDCGPGQDCAHPDSGCVKSLPYRMLGGLDDITETWASDVSMAAMVESNNYGSSDELAPLLAPSPPISATGTLEASGVLLDSPQASFRGSLDHYLQGLNTSSSSPSTVRGRIGRGAMTRMSVRAPAERSAGEICNNLEGMVDTFATEDGGDESGEDQEGLKASGGDDDLPPGCIIVEDATLDNSNLLPWPPRLAVPYGDGGAAGVIEPADEGGSNELSPTVVSAAVASPISLPDDGFKGSSSTTIVPGSGNDLEASTSYALSGRMRCTADPQALSAAESPRSDFEFGGSVPGNTGSGSPTLLATSSVHNGSGVNLQTFAATDSIAEIGFQHSDAVHASATATPGIARLSNRTSSPSGNNSRSDLSIVVSPPRVAVTVAATRFQSPQHSLSQSVSNKPADNNPYNDGSCFASQMCFRQLSNGSSVSSTTSVASVSSRSSGDLPSFHQVASIGSWLDHFGSMLPQPSIGLQRSQVQHDPVVWFTQQMAQHDGNRGMAATAVALEGVGPGLRAPMNSPLNSFDDNRHMGINLKGLASVTTTLFPAESVAPSCTAHLPSASPTAPSCVAEGGTIAASSSALRSPRSKSSEQVTSEFSIRRAPAAGGALSSLELSHCSVEAPIAGAVAIDSVGPGQCTKRDELSVLLNSPSSPVSPVRVTKFASPGVDRRIIHPSSLASPEGVPGFVQSKPTIRFNNNLLRLPQQRRVEGNNRCITATERAEVEALPLRVALPSSNTAQHELQGQSSLPPQRMFSSPSILRNVSSFAPGAVPDVAPGIAPDGQLDMSAIGATASGKSRGPDSPWTSESSSCPNTPTSRAADNASSPFRGPRDTSHSCVANGCVQQLRSLPLPGSLIASASTDNRSFEESFEVAVRATPKRSQVQSAIGRGSVHVSPYSSPYKSALAATMAPCSNPSPGTPFRSLFLEEEEELLGAQLCEDPMPLSPRANSLNVLFSMQTMNDKAASPGSVQVTSCDSDFRDLASNLVFAGTAAPLWPLKPALPSAANTTTCQRPSNSYGANTAMMVPVPGSSFADSGLEAVDYDQQTAKIQGMAAVDEPTQKSGSNMASLIPDSPHCMLPASTPESAATPDGQRITGLLGAKPELLSMLSCISSLADRADDSDGSASALPARAVTHLSEPNRQPRITLSGGLLDVPVVGASCGRMPKSNLSQISTPRQEDELEGLAGGPSMGPPSISDTMAGALHETQLQTPQPGSGSVIFSSMTTICPASKDDSIESSLGTFVAGCMAGGHGSAGSLASSVAVASRQESPPRSPAERTACPRCSCPSASPVKPPMQSVQSPLIPRSLATDGPQNQSLLHMPAPLSNPSAYPSPGASEPQVTPMKIVTSVKRTPTGAILGAADDLLQMPKLRSPSGRAGAVATSAFFGLSSSCSTAGLAESTFPRDRITSPPSQLSPAAVPSFGGMAPVRTQPLIVVPVGPTGPSASASAGQVPDPLPRHFGPCQAARHLWPPASSGGGGHDDEPLLATHASCPAGFVALSNRGLPPTPRSGRRDMTFSPSDGASAQSHDLYTQRSQQQQMWDRARAESRNASAAGTPRSARALFGTSQLPVSFSSTNCGYMEMCVSPVNAPRSPRVAASPAQPPIFAQSAVTSGTSGSSFVHDNYEGQSWRLQQARTMYQAVRGRDTNQLGKALQPPASPRASTSIANQSSFSVEPSSPSWSQVSRRLFPDVNSLGGAAATGSTVGIATLHRRSVDQLDEVLAHMKVQVASPVSPQPRPLSLSPRLLSGGNGLTETMASMGVATASTSSTGYRRPSMRAPSSKAVSSEQDATGVSPRSGMTSQTSVLDSGPGAFSSSIAATHLHCSQYSRDLVSDSGSHLPVAPMLPYLPPLPPLPRLRNLPRLPMVGPGVSSVDAMPAITTWPCLPLPLSNGGTSESGRDVVDMGRHRPYRQSAPELSQIFGPLLNGNPTRSSFIRDMSAAAEQDALMALESQFKAAEREAADLPIETRVATPTTLPQAVANSYALAKGDVAPAGLPVGGFGPAVVDAPLAGSLSDSYTVSQVAPPSPCAITASAAPPSIAVMRGLGINTTSGACGNLGGDGASRTTDRRVEVKPQAHASELFAETSVGNGVAQHQGQVDGGDRSMTNADLRPLEHGATVSDTKERCPSTLCTVHDSLAPHPNGKASGRSGSQCQQERSDHPQRGAPRDVAAPWGSLRWTAHAQGSSTTVTTPAETRGDPTGFTRSPAGSISGDLHIAPCARYMGQYLVPAAQMTADPRMGPAVWIVSNEDELVGALSKLLHDTVERQSRGRAAAQGAAKLASSLVTTVWNVLEDMVIAPALQDYIASRGARVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWI2</accession>
    <name>A0ABQ5RWI2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004546</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022105">
        <property type="entry name" value="DUF3645"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051346">
        <property type="entry name" value="OTU_Deubiquitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367:SF33">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367">
        <property type="entry name" value="UBIQUITIN THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12359">
        <property type="entry name" value="DUF3645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUF3645" evidence="7">
        <location>
            <begin position="174"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="557" mass="61964" checksum="3046AE9947405575" modified="2025-10-08" version="1" fragment="single">LIYACGAYENLPNLQARTAVMQALLHTASRLATVRYRSLPLLPDGAAVLEPPTKPSPGCFCGLRLLPGEVLEAHIHSLHERLAQDLMDEPPYELRWLKDHDMRDRIRRCVTDSTASADDILGPDARGPEQHQLADDQWAVVLALRGMLASNLLQHCLRKRHRVDFGINRTVSARKRIAVPYRAAHTPSERSEFAQPDVALLLTNISYYYDGLSLDEFQAALSTLLGMGLSAQRDIYGAWLRLAGNGIPPTDLGKFDEVDKVDTSNRQQMELMYRYLSHNMAVVDFWLNYRVYPTEMRQYTQRLASSAWNLADNGRAQVVGFSGTNDNHRLLPLQVRQADIPDSALRATNGKMLSVILKHTLGFNTLLPEDCSRGGRPLWQVLLDTALKERMHALLDCGALLAGASNRQASEYLCQRLAEQGAAAPFQGVTYFDEDKRNWTVSDLCGRRLPRHLSPIAERDTFVIYDDAHCRGADLQLQLSAVGMLTLGPGSCKDKVMQAAGRLRQLGRGQKLRFAAAADVAAKIVQLQANNGVAPCSHNRATEPAAVDVLRWVMRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWR3</accession>
    <name>A0ABQ5RWR3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="217" mass="24089" checksum="AFE9C40C2FCBBD98" modified="2025-10-08" version="1" fragment="single">GFDATAASKAAHGFEQSWLLLLLPARSMHPVNAAAANDVDAVTVMAVPPLPALCFRFNSPGEGRAFQSLMAAANRVKEAVVRRAEERQREMEQRQQRRAEGADRRQQRRKMRAQSRNQLQMVTAAAGNGALPSQPRAAEELQIADLESLLPALAPRPRAGGNRWSDDDIRVAIERYLLDPDFLALVERVEAIWDEMEEQAERQLQQPRGEEAAGCRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWY1</accession>
    <name>A0ABQ5RWY1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004694</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="670" mass="71326" checksum="CBBC165CF530FD4B" modified="2025-10-08" version="1">MPAHPVAPAIALTMFSQRRCCLLHCNSRGSSVILCLAGAQSSKAKDAKRSISRTERTSRPGSLPVFEKVTSLSVPSSSSTSPTVISPAFPTVPLELELQGLSPLDAQKRLTERVKAARTLQDFAAIIAAGGRLIDSICLVAILSILPRAVQRSAVPVVTMAAAHPVGQAGLPPPAGNAVGAAAAAAPVTTPAGGAGTQRSTLQLSDQETRELAGLVSGVVALVRVRLREFDSNGLVTVTVGLAKLSSYVAVEPAVFRTILTHLEPRLPGLAPKALANLMWALATAGVQPCGSWLSGFYNSLERQLESFSGSDLANLMWGLAKLDLMPEVWLMDQLLASCLAQLRRSHSVADGATLLTCLARYYCAYNFRVGEEALAAFLGALQPGLRAAAPGDLVAVVHSVVCMSHMPGRAFMLDLYAVLKDRLTCEPTLSYADFSRLLWSLSRVDYNPPRSWLREFIEVSAPKLPHLRPRALSELVWALACWDAQPSARFLDEYFRASRRRLSDYPPAQIVDTLSALAKLGCRAPAPWLGEMLTSFCASLPEARAHELVSLLESVVAVTDDRQWLAAPPQRAALQLLADTAASRFDAFDACSHARLVLALARANCCPGAAWLSEQQASLASAWSAEGGQSGGGMPAGIDTATAAGLREAYSQWEVELEPVLAAELEGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXF1</accession>
    <name>A0ABQ5RXF1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidoglycan binding-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004919</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="entry name" value="PGBD-like superfamily/PGBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidoglycan binding-like" evidence="2">
        <location>
            <begin position="339"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan binding-like" evidence="2">
        <location>
            <begin position="471"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="424"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="580" mass="60201" checksum="88EB800D86EE91ED" modified="2025-10-08" version="1">MLVRRPPQAGATNRFEAPVDRKAFGRHVVIAQRRSISHTPTGTCGRVLTVAVFAKKDYKDQKHIDEVAPFFSGTAELQAYIDRERFLLSELAATRGQVDKLLEQQRVLLDMVARLSGLSAPSGHQPLAISGGDSTSSSSSPAAATSPPSTIPSPPQLIQPPPSASPASSLSLKSPSSSSRITASSGASSTSTNVPALNSLADLFKSAAATAAHPASAAAAASIAAAIAAVDAGDIMSIAKFDTQPAEAAPPPATQAAPVPLPQPPVLFDTMGGFTAVAASASASATSVSYGTATGGEDSKSAMEAAEVPVEVAKAAPTDPPPDLVSGDDDIFWLSRLHSALEERGFFPGDDDMENWFFGEGTLAAVLAFQASERIPEIGVVDRTTWTALLGAEVAEELYSRTPTTTTTTTTPAVVTATATATATATASPGAATVTSSSGRLPAASGPAAATASGPPGDLNKWPVLMEGDGGREVHALQVALSNNGFHCGDDDMRWWQFGDSTLTALKYFQSCNSLPESGVCDERSWRGLLGAEAAPKDLYSVRMAVLNSDDEDGGYEDDMDGVSQGRVWLLGEQRWERRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXW4</accession>
    <name>A0ABQ5RXW4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Biogenesis of lysosome-related organelles complex 1 subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="21990" checksum="6436CFF6AEAED3D0" modified="2025-10-08" version="1">MLLPGGAVDSQVASSVIAGECRAIVARQRGVAEDIYDKLAASQAQLSHELNRLRNALEALHLPEPLALPADYVNALSRIQRKVSELSQRLSVVEARMSRIKARPSLHHKDGGAPEQRSSQAPMSFLALALKLQVVSVTHFPVPAGQKIGFIDSATHLISRNIVRKVGFWMLAFCILDSAGFQAAPTGRILDSVLWIFFANTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYL8</accession>
    <name>A0ABQ5RYL8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005442</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="172" mass="18072" checksum="A2170B58995CA4FF" modified="2025-10-08" version="1">MKTPAQNAWTTGLCDCTEEPSGCGFCLYACCCTPCAYGSNVALMEPIVCCGGDCFGACATYFGIQYCTGMACILHLMARGYLRNKYGIPGTGCSDFMITWCCTPCALCQEQRELVIRGHSKGGVVKGAQQAQLPPTQQQMMFPAGPASAPSSVVAPGYPAAPVSSTTYPAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYU4</accession>
    <name>A0ABQ5RYU4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="608"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="803"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="946"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="806"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1018" mass="109501" checksum="2FC9195137B56B5C" modified="2025-10-08" version="1" fragment="single">MPRGRPSRLQCKNVQSMPGPHLCAAFSRRYDRTQNPAPLYITLAGTLPEVFRYKIHAFADCRRRRWHQVPAPVSPSALPYNVPLTIPEPIPEASALPEEPPLRSEQIDLTLSIKRCRTWQDLRRLYWTNRTRHGVVNVVSYFTRLAALLPDLPELLGEEMYDTAATGGTLNPSYDDDDELDAGGDAAAGAVGFRAKGGGGGGGGGGSRGRPAAVAVGANAAARAGAGNAAESRHRQQQQRQRQRQCSLAERTALRQLVVRLVYDACIFTEDHVAAGRLEEALRNPSDPWDTEAYALYLTECDNTKLRCTGAGERAGVVNQLQDMAPPNRDRHNQQLPPQGQQQTQQRPGAPALPSPLTFWDWVRDRPELWSDPIEADVLRGLRHWDAPGREPSITPATSGGGKTWASPAAATPAAAVAESAQRKRVPLCYGPLEITTLAWAAARLQIHRAVPQHAAWLIYDIVHGSYRQMPELDGRQLAQLAYSLAQLGPCVPPGRWRRRFLAATRQRMGQMDPQSLANLAHSLEPLQLVPDPAWLQRFLAVSGPLLEADRLSGAELTQLAWAVFSLRKRLCRVRCWANELLQVSRTVVEYGSGLAVHPASSCTLSPSSSLWAPSRSPSPNGANAASASGAVTAPLLEFLPSLLAEVGAPLPLARMRELQQLLLPVNVPTPSGAAATTAETHGPAAVMTSHPTNVGNIDNSSYVREAAGRVPGSGTELDAVEVGNGAERWVSDRANPWVTDHATALLLGAVAGDQGDGGGGGAPHCRLAIWLLRQPPIVFLLMRLWVDSTRAVKALDSEVGRKTEVGSSWMKQQQLDANGEEEEEEEDVDAQLKELEAGLMQAQSIEAAFDFTTYVSRYLRAVGTPSKAAVPDAGGMAAPATIAMAPPGAAGKEPEPPATSLAAAAVASTSAFTATAAMQWQPPPLLQRQPHRAAFLHPPPSFSSFSSPLMTPPPSSMLSVDSPGEPPISGMAEVSDDVPTESTPMLLMLHTRCWPSTHWLEALAAAATAQLYTPGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZX1</accession>
    <name>A0ABQ5RZX1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_005953</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. Plant myosin class XI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01384">
        <property type="entry name" value="MYSc_Myo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036018">
        <property type="entry name" value="MYSc_Myo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF781">
        <property type="entry name" value="MYOSIN-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="14">
        <location>
            <begin position="8"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="13">
        <location>
            <begin position="62"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="12">
        <location>
            <begin position="1272"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="632"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1413"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="886"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1045"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="156"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI63183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1667" mass="184724" checksum="7DC372980C5BE778" modified="2025-10-08" version="1">MDATIIHTVGTLVWIKDEEEAWIKGEVIKVENGFVVVRTDATGAEVKCSSEDAPLQNRDNNRGVDDMTRLSYLHEPAVLWNLNTRYAYDYIYTYTGTILIAINPFTSLPHLYGEHMMNEYRGVNVGDIAPHVYAIADAAYRQMRKELKGQSILVSGESGAGKTETSKLIMKYLAYMGGYSDSGERSGSSRSVEEQVLESNPLLEAFGNAKTTRNDNSSRFGKYVEINFNKKGVISGAAIRTYLLERSRVVAVNHPERNYHIFYQLCDGASPELRKQLRLKSAQDYRYLNQSKCFVLPGSNNADDFKRTVCAMERVGIPPVDREAIFRTVAAILHLGNISFNPGQEDSALVTAATEDALDATAILLGVDKEGLRRALTTRVRQTPEGPIISPLDSRAAAETRDSLAKIVYAKMFDWLVHMINAAIGEDKNCAASVGVLDIYGFEQFKYNDFEQFCINLANEKLQQHFNQHVFKMEQAEYERERIDWSYIQFVDNQDVLDLIEGRIGILDLLDEVCRFVDAKGKDFAEKLYSAPSCKDSQRFIKPKTSNTEFIINHYAGPVRYDTANFIEKNKDFVVPEHQALLSGSKHPFISALFADTDAAVDGAAATGPPGRRGGAKGVKFNSVGSQFKKQLAELMVQLHAMEPHYIRCIKPNESAKAGVFENRNVLHQLKCGGVMEAVRISCAGFPSKRPYDEFVDHFWPLAPDLLKTDVDDKEVTKAILAKAGVTGYQLGLTKVFMRPGQMAQLDKIRTDTLNGAAVTIQRFVRGTLARWRFAAKRAAILKIQCAVRAWAARKLTAQLRLEKAALMVQCAWRRCKARSAYLEQVRLVMAVQSMFRGRNARQCLEQLRRMQAAVALQSIWRSTMARRSFLATREAAIVVQNGILIKHARLKLRKLRQEARENTKLLEDKKALEHMVHELQAKLETVQGDRNDWRQQAKEAQAAFAESERRAEEMRAQLEVVSLSEVEEKKQMLAEATQHIQNLQEDLAAQKEKLAASEEESARKGKEMQAALKKSEEYINQLMIERSQIDRKFHDMKSDLVTRLTNACAQRDEARARVLELENEVAKLLETIQVKDKELAAASAAAVAVQTVQTAAGPVGGMGSPGPVGTPIAPAASAVQNMFQKLQATAPGYARNVADNITGLFGKDSTPLRTPQRPGSMLAEEDIRSPIVGSTPGSVIGAGMESEQDRRMREAQVKQLAVLAEKRKAEEDRLLSALTLSPPNTRNGQPPEGAAPVAMGFYKGRPVAAIVIFRYCIHARAFQADRTAVFDRLAQTIGQQVEISQDDNNCLSYWLTNTVTLLHMLSRNIKPASGSKQSRGGVTAAGVGAAARSVLGGMFGSRTSASPGSVSPMDVASLHIGGVGGYKQVEAKYPALLFKQQLDAFVQKIFPVIRDNVRKEISPMLSNCIHTPKASSRPTARHGAAAHGGGDKAAGAGSGLQTAAHKSWTDILQVLDTLVAVIKVNYVPKVLVQALFKQIFRFINVQLFNQLLLRRECCSFSNGEYVKTGLEQVALWINGAGLDCIADSWDELKFLRQAVTFLVIGNKAKKSLEEITSDLCPTLSIQQLYRISTMYWDDKYNTETVSPEVLSQMKQAMVDSNSATSHSFLLDDDSSLPFQSAEVLASMDDKDLYTGIPVPEVLQDSEGAGLFTFLEKEMRFAAAVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZY5</accession>
    <name>A0ABQ5RZY5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_005938</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12236">
        <property type="entry name" value="RRM_snRNP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034143">
        <property type="entry name" value="snRNP70_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022023">
        <property type="entry name" value="U1snRNP70_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF5">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12220">
        <property type="entry name" value="U1snRNP70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="151"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="231"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="273"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="324"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="403" mass="46826" checksum="C9DDC8D5A7547C0C" modified="2025-10-08" version="1">MDRFDRFKKGGFNRQAAVKALKESVQTGHKTGLPPQLLALFNPRAQLPAVTDIKKPKIKLPYTGLGAYVKEFAGEGDVDYQPPRNEDRPPSPRLFRNRELAAQARVDTETKIEKKIRLTKWKIEEAEREAREKAKTFDPNKDPKIEGDPYKTIIVARLSYDVTDRKLRREFEEYGPIKRVRIVTDKQGKPRGYAFIEFEHKADMKEAYKAADGKKIEGRRILVDVERGRTVENWKPRRLGGGLGAPGREPKPPKKQRPGALPVASPVVGAPQVDDRSRGREYEMRDAPRRPEPRREFDRDRDVRSGYSGGGGSAPTPPAVGFREPPRDIRERSDPRESRDRERKREREPSPRRDDRAEHHRSGGEPREDKRSRRDLEDGELRDDRERRERERERDRRRERDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S032</accession>
    <name>A0ABQ5S032_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="906"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="713"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="757"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="884"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1144"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="572"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="635"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="654"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="714"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="794"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="913"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="934"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1163"/>
            <end position="1192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1360" mass="139755" checksum="0CECFE27EFADE35C" modified="2025-10-08" version="1">MSCFTACFGFPKRSPVIDASVVNPNPLECQAVPSRGMNCAAAISMSRCEPRASVASAWVATSPTYQRPSLKAQEDAVDAFDAVMSACVQLRSGSFGARSAAAADTEPSAGAAQPSASGSASANYLHPSAGRHARRSAQGQPGLPRPYASASAPIFKIEVPPDSDAWVPAPKQQQAGIAPLLLVDNDGPNSNMELGEFYESMLLSMQIRLKVDAELSSFRHLPSASSNRFIARLSMGQHAGLISAAASATPSGAWQGSGGATLPTNNSNNASRTVRRFDGSLDFPSPASRFPTSSISPSQAAASVMSPAVIDTASGVGHDGGSAAGATVSPQLPLTADCPSMTAATSAMVRALDHIAPSVPSQIPHLHPYVPPYLLQMFPSVGGGGGGGGSQQGSGRHAGMHRSNSIGRSNGTTHIAKARGAGGLRPLQSAELTHGSASPTSRTGTLPSSAEGVQPLSTAAAGGFASGPSEQRTITAQLVSKEPSEGPGGEGLWQEPNLEPAVDISSGATQLPTTPPPLLQAWGLGALLEATKSCGGGGGAGASVGAPGCTGAVSGGANCWEAWGDVSLTTQRGNNSTSATSLLPPNWQGQPSPPWALPPLSPYAVAAAVTAASGAGDSSCGEGMRVPPLSDAGEQQQLQQQPSLPASNQPQLPLHHHQHQHHHHGRQSRVLYNGVVGSSAAARMLRPIPESDSVSASRLHQMRESILLGEEEVAMQASESASSAPSPRAYQDSVKAATTPAVSPFSVVPSGRLMEQSPPFLLDGSPTRAQGGLSHPCQWSSAMQHHSPPPTPPQQQQQQQQQQQPPSGGPPRCFEQRRRQRPSAAKLPMTPSPRSASWYGGAPAVHPDPTRVAPEVPVEMRLCRAAQELLEGLSDVRPLGAEAAADAEDHKYRDAVLPPAGPRPHEQRRPDLGAGARQGAAAAAGGWVHEPLRGPGGGTPGGGGAECRSVVLQGRWQGHPVAVKLMIGAPLDQKNSALVSALAAAAVVHSGLVRVYGVRLLPSGAVGAPECRGLAKAAIAAGEAMAAAVAAASLKPGEGVLAIIMELCAVGSLERLTRAVYSPFRPNRSWPAYMARRALLRTALEVAGALAHLHRYGLAHGGLRPANVLLVPALQDRRKFCAKVADVSLSCNVMSHGPPGNPLLFFPSNSTGGRGGGNKHSESAASAALSLVPSPSSSAAQLPKQAQTTPSPLQGPPLPLPLALRPDELLFVAPEALRDPGALLASPPADVFAFGMLLWALAAAQTSWDSELMLLEGGVAATAVEVAAFGSTPGSYRPRPIPAWPSGAHPQLQNLYLACVSAVPEQRPSMDQVVSELEQLDEGLRNDRPKTRAAAAASYAALAAAGLPRGESGFIPGFML</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0G1</accession>
    <name>A0ABQ5S0G1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_005808</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0229">DNA integration</keyword>
    <keyword evidence="10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="13">
        <location>
            <begin position="159"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="12">
        <location>
            <begin position="468"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="508"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI62953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="532" mass="59928" checksum="7B844DDACFD897A6" modified="2025-10-08" version="1" fragment="single">WEYISGKLNIADALSRHPSLHAAILAAPVVRPRSEEVPIATDLAERLKVAYNADAWFADPANVENLHKQNGLWLRTEGAATQIVVPNDDALRYDILARFHEDPLAGHPGSKRLIDLVQRSFWWPRLAKDAANFVQTCSLCQRNKALSGKGHGLLQPLPVPDAPWESVSMDFVVALPKTEGGYDAVFVMVDRLTKMVHLAPCTSSCTAEQTARLFFVNVVRLHGIPKNVISDRGGQFTSKFWEALCKLVGMGVNLSTAYHPQTDGQTERTNRTLGDMLRNYAGRTPLVWDTYLTAAEFALNNAVNRFTGKSPFFLNYGFHPALPVWRELEVNVPAAKLFAKSFMSRITDAKSCLDAAQQRTTEYYNKSKQDVTFSEGQLMLLSTKNLRSLADGSRKLLPRWIGPYPVVRMVGNVAVELALPTDMTIHPTFHVSLVRPYRSTEPVPEPANAPAAIEPVPETWIAGKQKFYDVERVLDYRARRVGRHRRKRTVHEYLVKWTGYTDEHNSWEPARNFSQDMKPLLEEARKRATQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0W0</accession>
    <name>A0ABQ5S0W0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">U3 small nucleolar RNA-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_006146</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005554">
        <property type="entry name" value="NOL6/Upt22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035082">
        <property type="entry name" value="Nrap_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035367">
        <property type="entry name" value="Nrap_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035368">
        <property type="entry name" value="Nrap_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035369">
        <property type="entry name" value="Nrap_D4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035370">
        <property type="entry name" value="Nrap_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035371">
        <property type="entry name" value="Nrap_D6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972">
        <property type="entry name" value="NUCLEOLAR RNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03813">
        <property type="entry name" value="Nrap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17403">
        <property type="entry name" value="Nrap_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17404">
        <property type="entry name" value="Nrap_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17405">
        <property type="entry name" value="Nrap_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17406">
        <property type="entry name" value="Nrap_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17407">
        <property type="entry name" value="Nrap_D6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nrap protein" evidence="6">
        <location>
            <begin position="201"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="7">
        <location>
            <begin position="362"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="8">
        <location>
            <begin position="612"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="9">
        <location>
            <begin position="799"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="10">
        <location>
            <begin position="997"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="11">
        <location>
            <begin position="1323"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1206"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1218"/>
            <end position="1234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI63249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1442" mass="153684" checksum="514EDFE5A402916B" modified="2025-10-08" version="1">MAPKRARAASTPVELSDEDPMPSDDELQSQEVEDDTEDDTLGDVEHGEHFYHGSKDGDGDDPSFLLVKEAARRQQPAGPGPMSAAAAVAAPGKHGRVVANTALMLQTETSDAAILQIEANELLSETRVSYSEESGVQAVVGRMKDALLSMPVHQVSMSIAKGFVEELGGSAQDSLTIHPPAHVTVVGSYAMHAVARPDATVDIAIQLPNGLLVPKAHLNHRYHVIRAVYLVAVAQYLREVSYSLFGEQWIEAHHGDPTRPVLVLQPPKAEGYRLRLLPSIALSTFALPRLAPDRNGVRAHCKTPAGKAAAVDGSGFQEVPELNQQQQVLEPTPHYNAGILEEMLLPVHAARLQSVLSTSPRLADGLLLIKVWARQHGLLTTRPRAEATAATPSGSGNNPSTSASMGFGAAVGGCLDGCILAMLVMLLVEKNKMASSMTALQVFRAALQLLADGPSWSKGLSLGRQVDGLEGKVQHIAPPAMQVFKANFQVVFLDFTGWLNVAAYMTRGSLAHAQLVARRTLDLLTAPADPDEAFAAVFLTTANPAAAFDYHWRISLPAGALWPVVKAGDGAKGLQQSEEKHSGLIAPAAQIATVQNLAEGSDRGLLLRDRMMWRQQEAEIEKIVRQALSDRAKVVRVLQRPLPPMLPPEALSGGLLSWMGMSPSRAALNVADEDADDAKDAVATPRTSLVSSSVWVGAVLDPLLACRLVDIGPSADDGPAAAQFRQFWGRRAELRRWQDGMITETAVWEVAPHLRHIIPDIIVRHVVERHLPAESDVAGAAGCLDWALCSRALPPGADLSAMRAAEAAVDKLTKQLRALDQLALKVVGVQPLSAVARGTAPLPPPPHPLAGGSMAAVVGYSGGGSGVSGAIPRCLDPMEVLVTLENSGRWPDDPAAFRKMKAALGIQLSNALSLSYGHHVVTSEEAVDVLMDGFAFRLVLYSGRDEAMLARASAGAAVPVVASSESSLGSGALSPEESPLLLSWHHGLVSLVAGSNSAFAPAARLAARWVAAHMMSNHLNPQTIELLVAAAFSGAGSSVAPPPGSRITGFLRFLQLLSRWPWSLKPLVVDPSGELKEADRRLICARFEAQKSTGEAPAMYVCTPRDRESRHWTRKGPGDSALQRLTVLAARSATLLQLLLQPPANRTCGISSSTWCNTSASHNLDPSTPSGLAPEWRGLFATPAVDFDAFLLLRKEALPHADMAILPNPHNSQSSRPTHGKRTHRRYRPRRGKGRHVEERAVALAALLLPPGAAVQDLLYGGGACGSGSVVQQLDAPPMGPDALSDALEDVGPMQPPPKRARAFLRCFPDKVVVAKGIAKLQPELLIGFDPVGRLISELSIHFGHLAMFCVDGLGGRLVGVRWRPDAFLPQPLRLSSAHTALPLLLTDAAVTGDGADSAKKRGMGLCVPNVFAVLQESKEMGLGLVEDWLIPPSGVRARAWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1N3</accession>
    <name>A0ABQ5S1N3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA damage-inducible protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006359</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05479">
        <property type="entry name" value="RP_DDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14309">
        <property type="entry name" value="UBA_scDdi1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01796">
        <property type="entry name" value="Ubl_Ddi1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033882">
        <property type="entry name" value="DDI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019103">
        <property type="entry name" value="Peptidase_aspartic_DDI1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12917">
        <property type="entry name" value="ASPARTYL PROTEASE DDI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12917:SF1">
        <property type="entry name" value="AT13091P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09668">
        <property type="entry name" value="Asp_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A2" evidence="10">
        <location>
            <begin position="241"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="8">
        <location>
            <begin position="445"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="363"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="363"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="401"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="52680" checksum="C1D55F9D97DFD8E2" modified="2025-10-08" version="1">MQLTITTPDGEHVFPIEVDGSTSFADLKAICEVETGLPSASFVLVHNSRPISDSQTPASVGVQPGDMLILVQPAQQQQQQQQQQQQLQQQQQQQQQLQQRGTAAASSGGGTGTLMQRNTDGSLVNPAAAIQAFKADRSMMDTFRAQAPRLYDAIMGDDVAALQEELRRAHRSQHDANEELERLYRMQEEDPFNPELQAKIEEAIRRKNIDENYEAAMEHNPENFISVHMLYVDMEVNGVHVKAFIDSGAQMTIMTAPFAEKCHLTRLLDDRFRGMAVGVGSSKILGKIHQAKMKVAGQVVTTSITVLEQKTGPQFIFGLDMLRRHQCCIDLSKGVLRIGSCGVELPFLSETQIPKDFNAHVEDVSEDEANRRMQSDEAAATPPARADKMETNEGGAISGRPSPAAATQQQLQPPQSQPPQPQPQPQPTSAMPHRGVSGGDGGGGAVETKISQLMALGADRNTAIQALRAANGNVDMAASLLFEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3D5</accession>
    <name>A0ABQ5S3D5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GRIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF31">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN CEP250 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="576"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="634"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="669"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1183"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1530"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1773"/>
            <end position="1794"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="335"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="586"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="606"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="636"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1199"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1774"/>
            <end position="1783"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1838" mass="189925" checksum="1891FB3828449D25" modified="2025-10-08" version="1" fragment="single">MAPKPKVADLEAKLKAKEADLEAAQSALVKAEASRAKLKQYLEAFREQAISTTEIINRFKNLQQDYDSLQEKYQQLQQERKHPPTAATETGALPHDEGAGASGPDLAVASLEERLREEQQRGQQLQERCARLQEEQQAAVREAEQLRREHQQQLQSQGVSGSAAGSKKQQDIQQSVQKLLQQLQQEQRLNSELRNQLESALQQQQLLQLQAGESPVGGSAAAPRVGVAASSLPLGEAGKAPATTGVAAAVAHTQAIARKTAGGTSATTASRRRRVGGDDTSAVGSAAGSAAITSGSDSDSGGDGTRRCDGGGNLGRASRGQHRREWEIAAAQSRWKAAEAALEVTRREVEELRRREAAQKIEMDQLQAQLQALQALVSEHAAALKRQSVHLQQHDANLQRHELQLERHGSELQRHDSHMRECNNHLQRHGLQLEQHRDELQHQRHELVNVREGHSAETAAVWGQLEGVVAQLRGIHRTHSILRLAFGSSDAGCSAGDGSVDDHGCPGTGALNDASGQPNAGRLLPAPRAACRDGFGAPQAVRGETGAAAAVCAGPFSKKELLPQTQRVIQVAIDGGVAAPPGAPSEDDKAGGEQEQAEHHRCKRRRLEESDVATKGHADAVGMQPGRVVAKEYLPQQQQQQGQQQQHQGPTVHAHAVTVALRSDVVDASAEGKDLKRPAAAAPAGDACGETPTHRQGRGRPRKERTPSELLSTLLDGKATGAGNLTKVAAATARRAAAAIQANTLQPMPLASAICSAILRCSRDLVGVTAAANADSGTVLPPPLATAVDGLTTNTARSERGSGSRARGAVAAAAALGGSGGFLSLLLPSGRSRGHGGPGAATATAAGFGAVTLSDALTCLWCEPVEQQRQLVKWLLQVVGDTDRILGDEMTVGRGNGDSAAAVRAAVETQGGDGSDGVEALRKLPSSHASLRAAADASPLATVGSTGGTEIGAAAAVVEAEAREQPRSLQVSETSPSKEPMALLGSLVAQQLNTVACYPLEDGSPSAEAGEAADGDGLQGTVFVHGGGNGGAGEMPQGGGKGTEAVMGGTGAGGATRAPSLAQRCAAAYALGQLFRMYRDREGYQSTALELLHVTANACISNAPFNSGTTTAIAANRGCGQQPAIPASCPSEAVTTSRPTAHQTTGACADCSLVPLCCLLVGWPQALSSPLLSTSALPAQVQADSQISPARAETAAPPDHADVSERRPPLPISTDLGAAQGPRVGAPHAHVATGGRKAGGGLYEGPDDDRDILEDLFGDVESEAPHLPPRMGSDLQEMADASVWADPAWEAQLVLEPLGMAIATCGQLLALERLVECCIGGSTSMCSSIEGAAGCTDANSNGLQPTVTTSTVPHQSSSGSVLNGRLGSPVALDGHAEAVAAAVLLRALWEQHQQPPKGPGDQKPVGLLLPGVEEVLIAVGAIQTAMSSAAAVTAIEATREKGCRGSTDATVGLQSLANTRGFSKCRGGVGVGVEVGGKGRGSVGVDEDPSAVLAAALARAHFAISELASRLMGLLLTVMMERQQLRSRRHAHIEMQRQQEQQRYPPVDRAPGEEPSAAAAHCPSGSPEGPSHSRVLSTLMPPSGGAEAAYVANLRQALLLTCHLLPHEQVYDRVLRQLALWLYPGGPVAISSAVGSQQRKRSHEAVLLATATATATAMAGGLLADGNDGPAAAADTAASAAGSPLMVLGMEAADVPVWTARPRSGLPLPTADGTVATVDAASAARVAVGRALPCPEACSLDLETVRLVLEVIKDIACQVLGCSANYHGREQPFVPASPSASPSPSAPSPVPTTGSGTNAALQALLVSLACSSLCESLAGLLPALLQREMWGEDLVAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3L1</accession>
    <name>A0ABQ5S3L1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_007385</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024789">
        <property type="entry name" value="APC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024790">
        <property type="entry name" value="APC4_long_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX SUBUNIT 4 APC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12896">
        <property type="entry name" value="ANAPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="7">
        <location>
            <begin position="52"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4 long" evidence="8">
        <location>
            <begin position="347"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="620"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="628"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="652"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="916" mass="93849" checksum="CF6DFF5ECEE27B4E" modified="2025-10-08" version="1" fragment="single">MNTCEIKASETMHAAMSSGSASDDSDVGAHSNPNAFTVLYENALSQQVLLGCWCPTMDLLALVSDDGQLGVHRLEWQKLWVACPDTPITALCWRPDGKVLATGHHHGAVSLYNVEACELFRTLRAPLATPICHLSWVQGLAPNPATATPAIHLAYRYRHTRFFPAPPPGREQGVGSGTAAAAAGWRCGGAAGGAAGAAGGGAGGGVGAAALLEWPPPPDSLDVLVTSDTGGGVSIFACGEVMVASLPPRANDVRDVHHREGARELRSATAAAASGHLVARGELPGTLQALLSRDLNFLLVVSEPAAAAEATESGRGIRSSSSGGGGGGAGIAAAVLSVYGCGKLGSCQEEILELSLLGGLVAGRLEAAAAALVAAARAWKGAAAELGKRMDEQLREALASHGHDPDDALGELTCMLASGHVSAPLHSFLTGSLTEAGLRRLAKTVDLAVREVSSLLLDGVLPALQLVVFVMGELRGRARTASIIQTLGLQERAVAVAEVEAVKALIQVESLRQLVTRVGCQYRVFFTWLLKTLHSLEETRPPSAEGPLPGGPLPGAGSLADAPICLPTLLAFLEEGGQLSADAVARDLDASQSTLGLPVGSLTFPPDLLPHLRALQEAAFPMPPPEEPGAVEAGDLSPSDGGSAAAGPSRGTSGGSSAGVGEGGSGGSSWPDKPLQRQLGWLVELCRRAFGGLQKDVSPSVQLLTRIPLLPAPAPQRWLPYGGPSCGDRDGECGRRCSLQLPQIRTEPPVGKPALLACVLPAAALPIPLGNKDRDMLLLMQVALRPLQSRPQARACLLELPRGLRVLGMSYYRSAQLALLLGPTANPQVADGSGGSTAADGHGPGADGGALLALVPTESLRMSDVSDIGGGGNDAEPVTTVSCVAPLPPPSSSSLTHQSCVAQICAARGAVVQWPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3V7</accession>
    <name>A0ABQ5S3V7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="167" mass="17892" checksum="3C6721008CE8AD31" modified="2025-10-08" version="1">MAISSVSYLPGRQIATSMAQAVGAFSGRCFASAANQAPPPKRPSSIVEPTYTYMPPGSSMREPLYFSMSDPVYFVDPKPDSQAAAVAQTAGNAKAATPQDPAAPTSLRKFDFSTISGKHGDDGRPVSYRMADDFAQQGYVVNPNTHSMREPKYVSLSEPKYTGADKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S459</accession>
    <name>A0ABQ5S459_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="22574" checksum="043FF4F8E3111B96" modified="2025-10-08" version="1">MPKMVPRRRLYFPFHRWTINTSVVALLLTLLTLSCCEALRFGLDVLKHHTARQMLETRPMAGSKHLYCKLSGGCSQCQEKEEIERYCVDTGFKELLQCTTETDQEGSFPLVLTYPYPPDVHIDNDRDDVADGIRVVIRSCTEKTVEAHQEALQAEVAAAVAATGGSLAHLPLFKFELAMCGLLGVSLPVVYWRKIRIRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4D9</accession>
    <name>A0ABQ5S4D9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cysteine dioxygenase</fullName>
            <ecNumber evidence="2">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007291</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:20442"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20289">
        <property type="entry name" value="cupin_ADO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012864">
        <property type="entry name" value="PCO/ADO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22966">
        <property type="entry name" value="2-AMINOETHANETHIOL DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22966:SF61">
        <property type="entry name" value="2-AMINOETHANETHIOL DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07847">
        <property type="entry name" value="PCO_ADO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="300" mass="32652" checksum="8D461845021DB174" modified="2025-10-08" version="1">MLPFPQPLPLRLTNSVLRLRGCTGVTNHACTAAGFLRQHVSMHSQGAVAGSQSTSQQTSGSRLQDFFAAARRAVLRGTVNESNPETVTQLAKMMGAISLEELGLEEDMVSDPFLGALKLHLRDSRIKYMRIFEDHNLTFGLFCFPAGAAIPLHNHPGMTVFSRLLFGRLRVSAYDWVVQPAAPITSGSARARLVCDAVIEPANPPLVLFPASGGNLHEFTAETPCAVLDLLTPPYVPPERNCTYYRVQRQAMGWPVSLPGARRPVASQKMHVGDEVELEVFVPPDSFEVVSGCYPGDPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4X0</accession>
    <name>A0ABQ5S4X0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007618</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037">
        <property type="entry name" value="FORMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037:SF24">
        <property type="entry name" value="POLYCOMB PROTEIN PCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="95"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="380"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1152"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1357"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1683"/>
            <end position="1727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1835"/>
            <end position="1881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1925"/>
            <end position="1955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1995"/>
            <end position="2049"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="489"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1359"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1689"/>
            <end position="1710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1717"/>
            <end position="1727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1840"/>
            <end position="1858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1869"/>
            <end position="1878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1927"/>
            <end position="1949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2025"/>
            <end position="2049"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="2267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2267" mass="235757" checksum="453BA4AEB5A5C0C9" modified="2025-10-08" version="1" fragment="single">MAQPVQWTWPLLPWPASAKLADTLPVTEEERPPPPALPEGLAPLVAGLERVISDVEEGLRYGGGGGGSRWKVRSSYTPGVGTEAAEEGLCKEMDWGGEAPLSASSPSSSIQSRPGDPDPWEGTAAMPEVSLVCDTELNGHSFRVRTEDAVSGTEGDSTTGPDGRLPLADDGDAHLPIPARVVYGIMHLAAELRWSSAPPAGVVPPPPMAAAAATVPSLTEVTDSASALLDSSSRVLALLLPSNMSYAYAMARLYGRLGQVPAHPDVLAAMARGAELAAEDTLAVLGSLEQWQPPLPASATTVSVGSWPLLTEPCEATQLQGPLSSPSDAAAEEHKASGRGLGSEVVASTASAAEATAVDAAAGSAAADNIPIEKLIPGVMSSNEASGDRPLIGTGEEAALTAPPPPPDRPPVLPPSAAPLLSGLPLPLSVPAAVSVAAVARPSPSLPPSFSEQHHRMVEQGWQLAEGVRQLELKYSTTVAELSAARAQQVRALAEHDQLLRQLAEAQQRRAGLEEQERWASRQLAEAAPPQPYDLRDAVASALHAEDALGQQIYEHSVRMAEARDTLSRLRRTAGQTHRQLVSYSNRLLRAATSRLDHGGIPELRLQDRQQQLAAVAEVLAEHWYAPSDCCMLVPRLQQLVRHPMLTATAAEAGDLENGGMPRGLSGDLLDLRLACATAWLSSIPLATKRMSDGGGAAAAAAAAISTEVKPSVDCSISGGNNSDDALQEATALAEASSANDLRRQVLELVELTEAAVQRRLAAGKQAQVAAEETAENAAVAREPARVEFEGGADGLKVGPQVVRDMMGGGRKHGVDSGCRSSSGGGGGVLSLRSAYALTAACWNMGVMPPVLRNAVASDIRADVAAASPRSCDDGGRVGGGGGSRSNIPYGTRVSHNGALDAVVQKLFLSRVLGATAAPSRTAASTDGGNGDGVAALAQVPYEGRMLMAMQLLDMDVISPLDAVEMIRDTLAEVLPRLRDPSRLTQLVKLMVGPNTAATAASSSTAPLAELAHGPASASAIPPPPFPLPPLPPPPPCEGPGAMKISPSSGTDGARVAVGLAGRRSETAIPAKVAPSVMVMAAPAASAVDAVDSAAARARCRIAVLVSALPALASNPGLPLEVLRVICAGVYATQTALTEPALAALTSRLRSELPEGDREGETEEPDTTGAAPGAPNDPRVAATTATLADLAAASHQTEQDIRLDQVALVWLDQARQGQLLLQARLREQLLASWAGDATTALRNHVSAAAADRGGGGPDDGRRATEVILPDGAAEAGSGLLRWTRDEVDRSSMGWASGVTDAAQAAEVLVRLLARVTKRAPEPLSGHRLDLLQKAVEVALGAGLTRSDHERVVRQLLRLVPPPPPPSPSLPHAPSPSPTSPPVQPPMTMMRTSTSAVDVTSPPSPQLQMPSTAASLSMLPPLAAGRLLATVLSMPAAPAVQSLWAPVVEALLSSLDSNARCAARDLVSEHLQARRTNEPKAPYDNHAYVAPWSVGPMGHGTAATHGHVPGSSNPMSPLSPPSVLASLLRAPFVQAIGTSVQLLGSGSEAGHIGASIDGEDGLTQMASPFGLCLLRNSHGAIQRVLGAVMCEAATTGVTEFDILEPPELSKAGTAATEGMAAEEAMDTTCMTAGVQPQLLLLLDSVRAAAKLKRAAADVTAATSAAASATATGADVTEIDLHPQLRSQPNQTPPTPEIPPPPSHPQIPPQGLPAPKIEQQQQQRQYQQQQHVQTQDLVSGLDLGLAAVNRFISRHWDQAPLERLLQYGRDVRVLNSDVPRVVSLLQCAALLPVLREAQEGMEHLHACLQRLGDLAAAAKERQVLARQQLQALKIPRHQGHMQPHQQGAKAMQQQQQGQEQVPSSHHHQHHQQQQQQQHVVQLEEELASAEAAVTAVHERERTLRGSWRLQWRRLLGAMRQAVAQLEALPPLPPPPPPPPAATSPPPPPPPAAATGALCTPHVDLGRLRLLQAPAEVLRLAAAAMTSYEATRLAIHRRAVRKQPQRPVERPQPPSSSSSSSLALQILSPMPSSSSACSSSSPSSPSSSAASLATAPWVGRPMGRSSLIGSPGTMAATAPTAAASATAAATAPATRTATMDVVDAMRVLEEAEPQWRQLGGRARMDFMSSVGDLEGAAASEDLVLSAELLGKERDALWACQRRLLPYLADEISGELQAVALTAGGAISDGGGSDDGIAAACTALARLLSLPAAPPATLQLGHAAASRRGGDSAAAEVPFSALTRFRLALQGCKLAASRGNMHDGMAEITMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S517</accession>
    <name>A0ABQ5S517_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008383</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="121"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="316"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="398"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="790"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="842"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1065"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1503"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1664"/>
            <end position="1701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1750"/>
            <end position="1952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2018"/>
            <end position="2066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2162"/>
            <end position="2340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2427"/>
            <end position="2481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2557"/>
            <end position="2614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2641"/>
            <end position="2675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2107"/>
            <end position="2152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="908"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="933"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="948"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1065"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1111"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1686"/>
            <end position="1701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1754"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1764"/>
            <end position="1777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1867"/>
            <end position="1880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1886"/>
            <end position="1903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1913"/>
            <end position="1922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2054"/>
            <end position="2066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2212"/>
            <end position="2234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2294"/>
            <end position="2309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2456"/>
            <end position="2478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2596"/>
            <end position="2612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2641"/>
            <end position="2654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2675" mass="277757" checksum="B3BF32B7D2AD2B85" modified="2025-10-08" version="1">MKSGIEQLREEKYRFTIETQGLIYTSQNCCSATQDDGKAWSEDDSDRDVVPRKKSPPLAQRMARELTRVLREGRPGGFTDEALERLLAGSLQSGSDLAGYDVAKDYIKQVLGQLYDEDNATRSKPAETGAGSKHCAVEPPANASTAPTRFSSLESSSGSSLAASGPGPSLSNPMQTELSTADGGAMAPPWTTAIGRGAAEVGPGVTSAPAARGANADEMERPRPRPHHGRIKEQLDLLQNADQLAAIISVATTAATTAAAAAVKQTMQTAPPLPPGAYARMHATGRPMHNLPIVSAVMGRAAQGTLRRQLGAAADALGDESPDPQPQQRHGALVSPHQRSTPMVMTRRQEGGTSASGVAQGVGISRGEHQELGEGGSVMGPSSGEVRLKRQRLRWSQRRGLPLGPLPAGAEDGVRGAASPGEFHSVDTAGTGSSNLRVVRLGTPRVTARTPTPVRLGVPNELRSGFQPKMEADLAPMQGFEVHHEGTMLSARQDGTPTGDRIRIQRPDRPALIEVELGSGTQPIASTDTSTVAVAPRLVTAAHSPPPVRANLRPAPLDNEMTTIQQQQPAQDPFAVGYVSPGSLFVTGGESVFTRSQIVPGREVDANRVVAKGSGPAPPRPRRAALPEDMQDAFRSTLAFISDPGTARRQQRPAPAWAALEDVEERKQAQREAEELLGGAFDYDAVKRELEQAKRTSRLLQQAAGPLTPPASMGQVLNGGPELYAAGFRPRPTRGTRTAIGTAANVALQPRGDFEGLLPPPHSVWDVDEAWVDLDQFTKAVEAEVAYRAAAGNPSRRGSPSRAQAGPRTTAIAATTVSGKTIEAAKINDRLPAGRAISTAAVAAPASGGGKEQSDATVHTGGRKRTASPGPDTTAQRLMTGAVERSPSPPRIARKTQASGPFRIAFGRRVDEPPKAAEGRTPRVRSGSPPKARASHGRTRSRSTRQHASTPVAVAASPSPLSAGDAVCQVLSALASVLLSDGDEAEDESKIHPGDDMVVAAAGDLPGLMSSLTDAVPHRDTREAETEDGPTAVLAGEATRAEGSERTASSPSQTVVQGDLVATPLPSQHAHQQQPVVQGSDPLGYPAEVGITRDNSQEDSRNPVRPIQAPSGTLGTSDSSAATATTLVRQLSAQPWPNTTAASRPRFQYVIDTAHPPITHLPTWAASASAVEGAPAAYAAALGTAPTGLGFSAHARRQQQPPIATPMPFPTTVRQPANMQVVQPSNPSEINSALPQAGGDGNIIAATKVPYAQSWQHQEPQQQPQLAAQQLVFPAPGDLPSSTHILPQQAVVAVLAAADASVAGRRAPDPNQQWEAAVLRALEDEVVGRLAVLQLAAQGEGPMVSPQDVAGMLPPLLDMKLSRHLVCDVLRDSLRSLMVGQQQKGPPVGVEWQGPTLDEGSVGPPTFEELEGQTPSSLTQTTAAAHPVAEIIPRDDPLPTTVSPGRPMGFAMGAMSSVPTPTETNAAGAAASVVQHALSEPHPVRAWQEQGIAVLPGFAVASAGPAEERSPTKSQGTQAHGVQGRGVQTHGAEARGVQAYGVVVARAVQAHGIEARGIQAAGVQERGTQAAGIMDSSSQADPPVPRYLLQAALAAGLSYGAGPPLPTTIQTTGLGAGQPVVAQDGTALSMPRPFYLVPADGLQLPPTVSLYGPASGLAFPPYARGESSRPPLNIPGLSKLRDSSDVDSSTDSMGRHLQAPGTRARAAALEALRFASRCSLDSDDGLLQRSYAQLELASLTARPVRLDHHRTSLEDSQLSQQSETGLEQPTSRTTQHQSTDRGDIDAMGADASGTGGSGARVQGPIGLANGMDDGNPVVGDGWPGSQACRSERGPASHAIGSHAELSGTVPPKFLSRRSSLTSYGSADEPRPRSAGAERESLPGCSDPSNGTPIAQSTGHSSSALVDAVEHALSPSQATTSRAMNLDGRGQPADGVGPRGSGQGSALPSGELSTLSVEQGSVAIVCTPPAYHEGASGLQPAFTVAEPALTPAVGDDELVREGDFADDDPDGVHSVHFESSLDQVVSTPEEDKTTGATGVDAADDTSEAPAGSGAASRTTSFGAGTVQKQPGAMVGWTHQSDVDKPPGGQCSAGAGEAVDFALSDDELMDEAEVTRVLAEEDASEAAEDELEDADEQDEELRLLESQIVVQQESSLEVGFGLSATNEPVASGPQEHDSVAVQSSKEGPTQDAEVRFRGKGSGHGSERGGDSAAGSRQTSAMQLSEQSRIVSLESSTGSGGPDTVGVASRTDSSAAERPRPGSVADVGNTTAVSRQAGDKASDRSTFSDSCGNKRMSTGSAHGASSPGSSDAEGASELPKSAQDPDHLDVENQPYQSAASDADASEIALRTSLERLAFAGLAPMGRLQAGAGVGNSVLMRMTAAPVPLTSQQQQISGRDTMVAMSQRRSALAPLQAASVQLATSQADNGVALQRHDSPSKPPSLANYQGPTRQLWRPPSAQQTQDPLWTSSETVMASSEASGPTLDPLSRTFLDAGRRIPTLVTDDALAAALARRIGDSIESSVDLMTSIDSVPAIAARPLHTIAGATLPHLRGRIEQAAGSQSTRGIDFSHSAGSDVESEEEGQVADGVQQGSMGINKSEETGTGRRSSRREGAVFRLRQYGARRRKLMQQIGVNDPLLLSSASSLSTDSSTISGSNGDEVPMLLRGGLHGSTRRMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5G6</accession>
    <name>A0ABQ5S5G6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peroxisomal biogenesis factor 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_008031</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxin-11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF50">
        <property type="entry name" value="PEROXIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="26368" checksum="9A18A593EDB6390D" modified="2025-10-08" version="1">MASQEDVFDKISRFLAKREGIDKTLKVLRYSARLAVALGPKDQDLTKRLSSFEKSVGVSRKAVRFGKFLQDVNSLRHSKTKDATVILELLSYGGEGVYYFIEQFTWLVKTGALNKDLEGRLALASAVAELVGYVGSIWLKFHKLQKLINQEKDMVDQLQKLRKDEIVVDVKLESALASLRFQRNLCCALILQDIADSVMALNDVTNGRVNGLNHPAVLALAGLTSGCISFYKNWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5R5</accession>
    <name>A0ABQ5S5R5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_008737</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="96"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="399"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI65250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="443" mass="46829" checksum="C6E173FCB32A9831" modified="2025-10-08" version="1" fragment="single">DQGADATAELRALGANDPQKQLQAGAPVAAGGTPGPAAPAGGIGGARRPSGAHGGLYGPPLFRHDSYPPQAEPCRADVSTRTVSSRGPPIARFEDLRFVQQIGEGGFGRVYYGYWQGHRVAIKLAHPNAAVGADMEHLVREFGREVAAMSALPPHKNVLQLIAACTEPPHLALVTDYCAAGSLYQLLHSTRPGGGPPPWTQLLAICLGVAQGMAWLHRHSILHRDLKSANILLDNSGNAKIADFGLAKIYNGNRQALTGGLGTYQWAAPEVLAHQRYSEKADVYSFGIVLWECMARRLPYEGMTAVQAAVGVVNHGLRPDMPRDTPPYMTELVRACWAAVPEQRPSFTQIEVQLGVMLQQAQAAAAAGATGAGSEIASANSTPRRGPVFGGVHMAVMPLPPPLPSPPQQTLDPSVPPLQPQPQPLPMQHLRPVAVPMPAGAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5T4</accession>
    <name>A0ABQ5S5T4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Intraflagellar transport protein 140</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_008389</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI65019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056154">
        <property type="entry name" value="Beta-prop_IFT140_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056155">
        <property type="entry name" value="Beta-prop_IFT140_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056168">
        <property type="entry name" value="TPR_IF140/IFT172/WDR19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056156">
        <property type="entry name" value="TPR_IF140_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722">
        <property type="entry name" value="IFT140/172-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722:SF7">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 140 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23383">
        <property type="entry name" value="Beta-prop_IFT140_1st"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23385">
        <property type="entry name" value="Beta-prop_IFT140_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24762">
        <property type="entry name" value="TPR_IF140-IFT172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24760">
        <property type="entry name" value="TPR_IF140_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="IFT140 first beta-propeller" evidence="9">
        <location>
            <begin position="36"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="99"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="IFT140 first beta-propeller" evidence="9">
        <location>
            <begin position="166"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="IFT140 second beta-propeller" evidence="10">
        <location>
            <begin position="392"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="IF140/IFT172/WDR19 TPR" evidence="12">
        <location>
            <begin position="734"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="domain" description="IF140 C-terminal TPR" evidence="11">
        <location>
            <begin position="1228"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1384"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1384"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1405"/>
            <end position="1414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI65019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1414" mass="158007" checksum="865E45F44B7DB971" modified="2025-10-08" version="1">MEIYYTTKIAGRAGVIAGWSKAPGYPSYLAVALSPNAVGIYNEEGRPLDPNNEANIKNVRGTECAKLAWHPLLPLLAIGWKDGAISFWNAEERKLEEDSKIHRNTISSMTWTSSGDRLITGDENGRISMWKTDRLLRPIHVVAYDEPGAVIRHIVVGLPEEVPETNSQVLVAFYVADGTERATVKWCNDQGYSGVVQEMGESIHSLVHYPERDQLLVIGASCTLNVLARDEQLGAWATTSKMKFATGTGEAASGTGLQVAWAGNHTLASASEKDNVVRMYNFDTEDNYVLNLEQDSGLVSRVVCLAFDQRYHLLAVGTTDGRVMMYKYNEPSERSEPVLELAKCWEMQPAFFVGNRALSMEWGPFPRLMVVACSDAINVCRKTMLSYKYRDGVAIMQVAVDRVVLENMELEPQRPPGRLQLQDMQLLGLDLSKGLLLVWDGERAEVYKVTEANDIMAASQFETSSRCMAINNDSIYRAAENKLEVVNMAGTVKQTIMFDDNHGSPAFMDVARDFLVTITNLNFVRIFKVAGREAKPHAGPAPLCPAELKGWKVISAKVNGTGSMVAALLVGPEPARAVRLAVWCGESNTHHLHDFTPDSRVPVGIMWDTVEPKLLVVQTAPTFNESNLDEKPAGGDGSQGPDVAIIFVDIDKGSLLQEYQSIHQGGATACIGSAAPFLLTNRKCMVQPVPGSGGYQPFTSNVSRVLMTSFVGMQDSDEKTRRALLDFSFSLATGNMDEAFRSVKAIKNPAVWENMAHMCIRNKRLDVAEHCLSNMEHARGARALREAKTIEEADARVATVAVHLGMIEDAKKLYIACERYDLLNQLYRACGQWDKALEVAEKNDRIHLKSTHYAYAQYLERAGDLTNAQKHYESSGCGLVEVPRMLFEAEKFNELQAYIQANDSRELILWWGKYLESLGEYGKALDCYRKAGDSLSMVRIYCFQKEWKQAEEEVANSNDQAGAFHLARQYEAVGRIPEAVRYYTLAKRYSHGVRLAKVNELDSDLMNLALKSTPAVMIDTADYLFQKGQHEKAATLYMKGGKLSKAVEMCFQAQLFDVLQHIADDMSPDKSDPTLYNRCAEFFMQFGHNDKAVKMLIAAQQYSRALELCVEHDVPISEEMADSMTPDKSIMKPDERNGVICRIAKVAKRQGNYQLAAKKYTQAGDKVKAMKALLRGGDAEKIIFFAGVSRQKDLYLMAANYLQTLNWHSDPELMKHIISFYTKATAWDSLANFYEACAQIEVDEYRDYEKALQAMREAAKYIAKSKSDDRDQRLAAISDRITVSERFVEARQLIATNPTEALRVCEELLQTIPQDSQDLEAGIRIGDVYALMVEYWYEARQPMEAYKAIEAMRRRGIILSPYLDTRMVEDIYKALGVAPDMAEDRRGPADLGLRGERDTDNGGFIEEEVADDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6T7</accession>
    <name>A0ABQ5S6T7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">adenylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009113</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="543"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="550"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="565" mass="63673" checksum="B35F20B4AF3A12A8" modified="2025-10-08" version="1">MLLSCGPLLNMAAPVTQLVRLAAQPLWSNFTAFERPMEPVTSVQPAAGPGPSSQRLGGLALWNIASNCEQRRHCRWLGETRCQTVTAVDLGTPQANTAVALMESKVHSDADVRMDAQVIFEACWRRFEEKFRLKSVNVPREVVWLNGAPGAGKGANTQHILKTRGLDHSVNLSSLLQTNESTRALMERGELIPDSMVGDVVLEALLVQSCQIPECGVLVDGFPRTAMQVDFLKLLYDKLDELHGIFANTTHRSQFPRPLFKVVMLYVDEDTSIMRQLERAKLASVHNKRVLDAGAGQLWEQRSTDLSIEKAKKRYAIFRQHHSAVLRLKQFFPFHLIDAMGTLAETQEAITTELRYQSSLDLSYETYASIRHLPLARDVSQHARQQLVMRLDNHSERYPDLFQKVIDLITSEIMPLIHECGLAGRAQYATEHRMFLEHPRAAQMLIDVLTDRGYNVSHSTEVTHVPMQIDLQTGLIKNRKEYKHRFHVNWDTKGVREMARAMELAARISEVGGGGNGSARISQTYIPQQLNREMRSSVLRQQLAEGNGKPLEEAEREAEHVIHGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6X1</accession>
    <name>A0ABQ5S6X1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Erythromycin biosynthesis protein CIII-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010610">
        <property type="entry name" value="EryCIII-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050426">
        <property type="entry name" value="Glycosyltransferase_28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050:SF11">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06722">
        <property type="entry name" value="EryCIII-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Erythromycin biosynthesis protein CIII-like C-terminal" evidence="2">
        <location>
            <begin position="711"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="593"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="855" mass="93439" checksum="FF1D8A65C7D4C29E" modified="2025-10-08" version="1">MTASSTISPLHICCIALGTRGDVQPVACIASHLQRTHDHVSVSFITHTAHSIWLESGVYSNLRDRVTYIASKPAGGCWTPQAPPTTPGLRSYGDPSAASKFPYVTGQEQEQGQPQGQTSVAQPDPWVIDAICNCVSAFADDMPGTPVPFPTSSSSQSPPPLRLPVPTLRTSPQSLCTMLRQRVRKLRQRLFSQSAHAQEGARRPFEGPRKQKFRGLQGPKPLYQETEEEEEEEEEEGRKPRPKQWLLEHAPSGARLQQQQRHVIVFNLFALEAYHAAEALGLPCAVAAPYMIPYRCPAAFVRMFRQELPDLYDALSWSEKLLLQQQQGLKVDEGRCYGTVAVIAAADTELAPKPIEASGRARHSRITFSEVTHWMWPLFTERWGEWRRSVLGLPALPLHRYHVETHGLESECPGGAGDNRAAHYGDGEPGDLDLCGSEEALPLDCLPPAPPLLYGFSELVVPRPPYWPDSVTACGFWQPPLEWFAGQQLPTELLEVLRAAPPPPPATAGTTAPAVPSTLGAPPLSTTEAGWCLIDFGSCGRMGFIPAPLDTLTLLDTVLHQLDMRAILLTGGWQPFHLAVAELHKTSSRRGRQLQSQQLREQELPQQQPGPPRQREQPHSVGRGKRRLEVEVELELDAGVEGPAEPLESESTVAASHLVRNTAIDDIEAAVEDPTGNGDLKPAQLPMQPQPQPQPQLPRIHLYGGDVAHQLVLPYCRLVLHHGGSGTTAAALLCGIPQVTCPFHFDQHFWAERTAHLGVASAPLHRRLLFESISTGPNLRHTSRGACAEPYASHNEVLMSNDPRVISLTAAFRDALEPSKLKVARTMAKDLAAEAGGLQTAAQVLLALAAGRQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7C4</accession>
    <name>A0ABQ5S7C4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009421</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1057"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="22"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="348"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="653"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="790"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="897"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="995"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1392"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="354"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="802"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="997"/>
            <end position="1022"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1438" mass="147997" checksum="C935C9C7DEE7026F" modified="2025-10-08" version="1">MPSHDKNADAGAVRSLLNLFRLGKKKKEKGKKDAMKTGLGEPPAGAGPSSDLPPAPEVFVNTQAGTAPEVTEVASSAHRSDERVEIEPGDAPPVPVQHEKLLAALESNQNTADPLQNSARMSFNVADLSGILFAQRTNTQSGTGMVLPARMGEGAASGLQLASGVNSSEAPLQRILEDGTPGSYAPYATTTVAPIHSTGNNMIQPDGSNTLYSVANNLQEMLYADYDTLERIQTRQAGRDSGSGAIAVGSSDLRREVMKSARLPVGLGDLQVLTTNQASDEAARPAIAKASEAGMGQGSGLEPALVIVAAAAAHTSRSAPLVHLQLQQQQQQHAAGRLVSGMLMGAERSPSRLRGNQGSPVVGQAQGTGSTVRLQTDGTAQAVPAAQTTSSPLKSSAPPLLQQQQQQPGEPQHLHAKAVAADAQLDPAQDGQREAIFCVLAGAEASVGLTAAETAEKPAAIPPQGSALEASALEGTSAAVSAAAVLPPAAVVATAKHSGGAAEPPRIIHPEVNLRRVPAPKQTSVAAFASGGGGISSRAGAFGSGGNPSVTHAQDFASVVAGPALPSYSQRPAPLAELAAPTSRPMKLTTACLSAYTTLYDNGADQSFQRALHRKMQLVYKWMQASDLTAPPDPRLEVPLDLAAAASEGAGHLLSGNADGGTSAADEGLKGVQGKDASHAPSEAALAVVMGKGHCMELTGIAAAAPAGVTIPLQEKLAALGLSKPAARSGLLTGDDGGLAAAVSLPAAGPATAQEANTNGAALNPHLLKQPSPADLDSTFFHHHHLPQATQHYHQEYHQKQHQQNEQQTEELGCTELPSHAAAGEARADGAAIAAAVLAQPVSCGEYVATTIEVQSYSQPSPALKVVQGVVPTLTAATAESVGQQLQLPLQHSLSDLYGNPQPAHGSKLPPRMLAGSEADPASATVTVADPAAAAVTPVTGAGAAAVLPAVSRIPGRSTAVTTTTTTTRPVVPVEGPGLLLPATGDVVVPATAPAMQQQQQLEQQQQGAASTSAQSSNSGAALPSGPGRTKTIAVASHCPPELQPQSPSASWTIDDFSLLKKLYEGSLSVVCQAQHKRSGRHVALKIYKRSRLHEMERFQLAREICLHIRIVHPNVVALYAAWKDAKYVYLALDWAPLGNMFDFLVSRGGRLSEEEAARAVMRPLMSALAFLDSQHFIHRDVKLENLLLDASNCLKLADFGLAIDQKFEQANTRLGTFGYFAPEVLDCPLKKGPFDLKDASAPGYDSRVDVWSAGVVGYEVLTGRAPFSASSPAKIIQAIRTRVLEFPAISEEGKDFLRSALTRDPATRPTAKQLLSHPWIVRYCGNNTAVPTLPPPSALAQQPLLVSKSASAGASAAAADALAAAVGLPLTVDGVAIGSPALGGKPLAGPTTGPVAAVLSSGGTEAGVPPSKAPGGASLFPSQGKAEVDRPPLASQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7V2</accession>
    <name>A0ABQ5S7V2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045108">
        <property type="entry name" value="TXNDC17-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010357">
        <property type="entry name" value="TXNDC17_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12452">
        <property type="entry name" value="42-9-9 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12452:SF0">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06110">
        <property type="entry name" value="TXD17-like_Trx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="50"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="175" mass="19083" checksum="41BF740D7FA4BD5D" modified="2025-10-08" version="1">MLLVQLQRPFPQRCSQCQAADTRFHPLLGSRRYHAPNVRTMAGKVLTANIDTFATAVSELVKAPAPHYIVFTTDNDPSTGKPWCPDCVRAVPPIQAVAARSPGTLLEVTVGPRSAWKGNPSHPFRVDPQLKLTGVPTLLAWGPSGAIRRLGPELETCKSSEEVDKLLSSTRFFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8Q0</accession>
    <name>A0ABQ5S8Q0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35169">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35169:SF1">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT FE(2+) 2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="668" mass="70031" checksum="AF127C5B430A9381" modified="2025-10-08" version="1">MESFTHAVETPRPPQEQAGIPSSADVAPTITASVPSASPNPLNLRPIATHATSWRPTDSRVTLNVTFDACIVPDATGAARVDKAHLVVLDNFIGEAERTGLLDFITHPGWNMELPPPSDKWDRGTRDSATACPTWGLRDEWLRNLATNPLPARLEVQSRLQRLYPDYVIAHMPSDKIQIQTRIQDTAARQDLARNQIPADDANEEAAPPGSIVDCNQFVANAATFGDTYSWHVDADPSTLPYPSPWTLAYGQYVNREPGRPLFVSLLLYLNHSWERDWQAETLFLDTPSDCGVVVRPKTYRAVLLDQDILHRLIPPSRAAMGRPRYSLVWKLVMLPRQPGHQPTLARRAWGRPTPFGSAAALERVVSGLSAAKAAEADLAMKAAAAVGATAAEAERGAAAEAGRGAAAEAGGPVAGVGAAAAAAAPSRRKRRKKQKSRDMVTEGQPHAATAATAGGGGGGGNGGLLAAVSPVPKPTVAKKRRRQGKAAADRAPTANVTDLPGQHGAGDTIPRSRQEKVEGGSMYDVTHGVPLKLDRGIVASEGDVGPGFGAGKMAPDRSADGVTEPTGTETPDGAESDFRQVAAAAAEVPAAGTKKQRKLQRLRAAHRLKKELKKASAAATAATAAEAVTSAGRQAAAEVAGGAAADVVVMHDVAADAPGAGGVLVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9A3</accession>
    <name>A0ABQ5S9A3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009358</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="140" mass="15570" checksum="07CE82A25578CF68" modified="2025-10-08" version="1" fragment="single">VCRHLARRSLLSQFSWQHIANTVWALATLQHHDAEAMEVVEQEVVARLGEANTTEAVGGTDGAVNHGRELPSTRGLRSQHIANLLWGYGTLEHPAPGLFFAVRPTMERRVAEFSEQELSNSVWAAARLALYDRQLMDVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAM8</accession>
    <name>A0ABQ5SAM8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="574" mass="60151" checksum="5C78C752710E92DA" modified="2025-10-08" version="1" fragment="single">MCGYVLKAATSRHRQAKEFVEGSLHAQVLRSRIHITAAARGQRQQALCMGRLNLPSSEHRKQHSLRQQQSIYDRPKRGVNTLRTARDPRVLAKASYVNNPNDSRNGTTREHCGNMGIPPPPPLPPPYRHGILRRAPPALIMPLIIAVVTVSSGRLALLPPAAVAWLLCTLLSVGHTPPPAWIAEAYDILTNGVDELQPGQAATAFLAIARLAVSSSRTDDLVTTATGNTAQTPPLPPPLPPPSLLPGLYVAMARPSRTGSLGPRVALACLEEAAAAGGVDRGVHLPYESCGTAAVRQDFSQPQYCSNASADTSPRMPGIESKDQRVRSATNSVGVFGSDGNSRGAAGPLEALVAAEAEQAVLTGGGVEHPTSTDLWVVDILSQEQWRCSWNLSPAMPRPLVELLVRRAVRLPSFEGGESVGAAAAAAAAAEPKVCGPVAAVAAARAVIAAAHLTHPPRPVVLSDIATLIVENCGSGGPFVTTAGGAGGSPRMLVHALSALCTLSTEPLPPPTVETLVRGLDDQLTELDAVQILHCISAVQRLAAEAVPTAAERFARGPAASELTRQLPELSATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBB1</accession>
    <name>A0ABQ5SBB1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006917</name>
        <name evidence="3" type="ORF">VaNZ11_008083</name>
        <name evidence="4" type="ORF">VaNZ11_008525</name>
        <name evidence="5" type="ORF">VaNZ11_010942</name>
        <name evidence="6" type="ORF">VaNZ11_016586</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="5 7" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000049">
        <property type="protein sequence ID" value="GLI66911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI71391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13223" checksum="520D8264A251CA19" modified="2025-10-08" version="1" fragment="single">DMACDDPDEETPAPPTKKPRAASVRPGITGHEDQPRQVKGAGKSKRGGGQQGRHLAPKGGHVQKKGPKQNLAEYLTTFKCTRREFHFRKQHGMCLRCGSDQHRVDSCPMLGAEKNKGKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBD0</accession>
    <name>A0ABQ5SBD0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PSP proline-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_011384</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052115">
        <property type="entry name" value="NEXT_complex_subunit_ZCCHC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006568">
        <property type="entry name" value="PSP_pro-rich"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13316:SF0">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13316">
        <property type="entry name" value="ZINC FINGER, CCHC DOMAIN CONTAINING 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04046">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PSP proline-rich" evidence="7">
        <location>
            <begin position="495"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="161"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="164"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="704" mass="74390" checksum="248CE424003874B2" modified="2025-10-08" version="1" fragment="single">MSTFRAPWQECNMPRPVPFPAAHRPSAGPIIPAPSMAKQELEAFLREWCRKHSIPGQIWDPNQQQGQQQQQEMQQVYQGLQHEEQRGDAAADPHNAGLTRERCNPANPGPLWSGSLNISWSLYPQIPSVGQVRCSEQHQWSSHEAAASWRWRDRLWAHDVPVSRSGQGRPTTGSDDSGPVPGGVDPMWQSGAGAETCQGGGGVDTVEKGASSGESGGSARKEERERTPGPGCGKISGVNNGDDNGGGRGHISENGGAASIGCAQREGDRACGGNVGHSVTLDSDTTMRQHSLFNLKDAIAFNLAYQKENNTPPYNRLPVLPLRLGLGLAASALSWPPLTHSSAGWQLLGATSNAVVDHGWRLGPGDGKVHLRPLKRQRMDNNGGNTLGQNFAFVLDRCWNCGSYGHDLQGCIRPRNHAVVDACRRESSWLVRRTARQAVAAQQRGPRKASYRRYFSMATAEEEQEATAAACAEIEAIPACCGNCGDLKRAPLGIRPGGLSTALALALNLSCPSDPPPWLIGMAVHGIPPAYCRGPYIPDATERLAVAMDPPSPEPCDTAAWEEPFSEALCGAPVTAATMAAAESCGQDGNDGCDAVVVQAIELLDGNPGRAAGDGAEVLPPKAMDLGCPKKSTFTDMGTISALEKWVVKQADITAASKGSSGGHMGCCKGDDANGPLVSGLIAALPGLGPDSEGSEGTRSSRAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD42</accession>
    <name>A0ABQ5SD42_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012137</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054835">
        <property type="entry name" value="G3PDH_Arsen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052978">
        <property type="entry name" value="GAP_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033735">
        <property type="entry name" value="G3PDH_Arsen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42955">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42955:SF1">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="4">
        <location>
            <begin position="4"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="348" mass="37648" checksum="F5467BC6E9661BA9" modified="2025-10-08" version="1">MAPVKVLLNGCGRIGRLAFRVAWAQPDVFQFVHLNDITAIESVAYLLKYDSVHGTWAPDVSVEGNNTIVITDGDRVDRIPYTSSKTIDGIAIAEGTTVEMVYECTGVFLTRAKIAPYFDHVGAKKVVVSAPVKDTPPVLNVVVGCNEEKYDPATDHIVTAASCTTNCLAPVVKVIHEKLGIERGCITTIHNLTNTQTIVDAPNSKKADLRRARSGLVNLAPTSTGSATAIALIFPELKGKLNGLAVRVPLTNASITDCVFDVKRATSEGEVNQVLKEASETYLKGILGFDEVPKVSTDYVNDPRSSIVDAACTQVIDGTMVKIYAWYDNEYGYSCRMVDLAKYVAGRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDC3</accession>
    <name>A0ABQ5SDC3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="167" mass="18076" checksum="0A45CD62E2A00ACF" modified="2025-10-08" version="1">MADTSDKLPFMQQVWLNRAATIMPWKEATSLIAKGADEKHNLVLALAGLTDGQRAAMVFSKWFYGGQSGPRKTSWFPPRPARLWGPPGHGVQASSVWAAATVGRPPFGANQSPRVFVELTPAVAIFQAGCDGPSLQVKIFVYVVCDFMALGLTHAEVQGAFDVMDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDE3</accession>
    <name>A0ABQ5SDE3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="352" mass="38241" checksum="613CED31AE130835" modified="2025-10-08" version="1">MSLEGKVALVTGATSGIGLETASALAGLGAKVILGVRNTKIAVDVVKEIQEKHPGAKVVIGPSLDLISQESVREFAEHINKEFPQLHILVNNAGTCFMAKHFTPDGVGGIAQTNHLGPYTLTRLLEKKLVDSKARVVTVASVTHRTVVLKNALSFLTDWRSGYYQHTKLANVLFAYELQRRLGNHGVTSCAADPGGVRSNIWDKSPRFKQSIERAFINACYSPPVDGAKCVIHAATVPWEKDRKVVNGNAVMPTYDLRYYSRGLFCSPLLCKLDGYRGKALGTAQNIKGTIWGISTVVISLLDWPLRKISGGFLASTCKPVRSATQTYDTKLASQLWDASADLAKLPKEPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDK6</accession>
    <name>A0ABQ5SDK6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_011613</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044235">
        <property type="entry name" value="RNFT1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15860:SF0">
        <property type="entry name" value="LP20373P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15860">
        <property type="entry name" value="UNCHARACTERIZED RING FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="34"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="299" mass="32501" checksum="B110387F07389730" modified="2025-10-08" version="1">MDSDPSQERPGINASDVTWERLLHTLHGSSRAAQLSFLGNPSPGAQEEANPGYRVTPSLTDSSPSRSQSPEGRVDHEMHSSTSTPSSSTPQQQQIHNHGIYRLYAHQQQRQSQQNSDVATPSISQSRNLLLLGQLSRRAAAEAGASLVNIVDGLDSDAASVSASAARHRLISGTTGISAQMFVDWVADLTPFLLLLAWVFIFEHAGALLLNMILLTSTVNVNRDIRRIIAMRRDARVSVCITQALTVAVFTSAAVLSTLPDTRLLRVLTLRATEAASDRAPSPRWLVRRGYFGCRTVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE58</accession>
    <name>A0ABQ5SE58_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="354" mass="40781" checksum="23207DCB51816AEB" modified="2025-10-08" version="1">MWNNLRTALLHYFRYDPSDFSADANNHAARLLKSFSIAAEHVFGIQFCTYNLHILVCRLREQENARGHVAFATEFWVERGMQMMKSIVKYRSPTVPELVLAGGYLASLWLTHLKAAHPETFRTFDEWVPGYRADNIQLNPSKRDVFLDEEDSEGSQLLGQGYIADAEQRSHILGGILHTEETEDFMARTNLLPSVLQDSDIYIFKRALKRRDEVVLSVDYARATKRQSYHFKCKYGPGGMVSESDPRVSIFIGEILFFALIRPRCDVAPVKVAVCNLHRAEVLRPKELYRVKDYSRRDATVNEVRQEILLNYFPKEVGMIDSKVIRCRVNNELSYFLTYVTFSQLPTNDGGEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEI1</accession>
    <name>A0ABQ5SEI1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012723</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010297">
        <property type="entry name" value="DUF900_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36513">
        <property type="entry name" value="ABC TRANSMEMBRANE TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36513:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05990">
        <property type="entry name" value="DUF900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="767"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="965"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1172"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1294"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1329"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1456"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="561"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="788"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="862"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="890"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="994"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1300"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1340"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1350"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1400"/>
            <end position="1414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1632" mass="170351" checksum="59D5B34A6A51A1E5" modified="2025-10-08" version="1">MEEVSRAPAGQAHHAVPDSPVVLNLNIVLPYMQYRKKLRFFNGMYTPSNVSRVSFMLLVIFVLWTFQSGFIERTTKSYDFGVVLSLSYSFPNGLTPGSTNAIILNIITYMLMAFMLIMVAVHVNRYHPFMQALGVACVVLFGVVLSRRSHMPADVAQDGKTLSNGFFLTQDLVMQIFVWVGLAYMVALFAVRFATPMAVRRNWLLFRCGITQRHDLTDPAAVASLIDFPPGYSLTTERRLVACVIRYISPWYAPMMGHHESVTYVGEVDAAGNPTGYGEWRDTHWHGEHLTGFWHRGIPTGPFRSRETGSASGFVNLRIAYFRNRGEALRKAACLPQLKPLQFGIVACEVSTSGAWFKSLPRLRELLPPVDIAEGLIAEEEAAIVEGRIAPAASHRAGLTPRTKSAQTCAPQPLPRLCTPRRSTYSGPDASSTGVSALFSRRLPVHIAMRGPDAGQQSRLVAASYPDAETPLELQQPGVGDGAVKGTPVEAAAAAPTSTSDNVGAVSGGNVPKDTSQGASGVPVAAATAACPEPTLKLALEQGNSPVSSAKLTKPGRSLDSATRSSATTTTSTATTTATPTTATIAVTTSIATTAIVATAATAATAITDITVTSAPTSTGPGRSHSAVLVYPVSGSAPSQVQRGPPPTRIPTLARLKSALSGRAHDGVGLEAGNAPPASSCERGAPLRNLSGFLQAEMALQAPSLRHPGGAAGITLQPSITTLQAAAGERIAAPSAPADAAPTASVFFNGMGHVPRLVSMLMPMQPAQTTTVHRRNPLRQSPPLVVGQGAGPDRGSTGGSGRAAGADTERKNSRWGPGWGSETDVGLRHRPAGAAGAAGLPPSITKGSSQRLRWRLRSGFRAGASPKYGSSSSASERTHYGRRSASPQHPGSMAPGASVASPSGPAEAAAAAAAEDGDGPVYHRALISFIRSSQPFAVVGGHGSAAAVGTHGSSWPEGAACSGGVARSGHGSLQPIDESRTSTCGSGPELRTPTHGSPSGSMHMNAPSERSSNCQRDESLQQGTTAAGKATPATFGDMPEMKRREARPPPLNFTFDRPPEAVLFVHGLGVDLHKALRHYAQMMALIGFPPYITPVLFSWPSSVPVAYFWARYRGAEPPATGLSLAAAVRALAADGFAGLHIMVHSMGTRVLANALPHLEAILGEDGAATTTTTAAGNGGNTGSEGYSCGPDASGNGSGSGSGDTSTRMKLRTVTICNPDFGLKRFVEDMGPRLRALCPHVTLYGDTADGALGLSEVVNALARPFVNAGAGIADAGNTVPALVSEGGVSAVHRDLGEQDKQPVQQRASNASEMLRDTSAAVVRAAAAAVKKTQSASPDEPLAAAESVSELPAPPPPPPPPPAPAPAPPPPPPKSAQVPSPPQPGRRTNLPPPLLLEMQAPQPQPQPQPQPQPQPQPAQLEILEGMGQGAGDTLGAAKVAAVLRSNVAALTAGEAGEATGLEQPNGGGSSAATAVCPPAGRQVRDPMDHGAASESGHGSGHGGVAASAITGAVNRSVADLSWWSEQVVNYRRPLHRPPLYGWLEHSLGRRIYQIQDPATELPLDIDVIDLSYISSNVNSTRHTHFALNREILDDLWEVIVLDRRASQRKSRLEHVTGNVYSIAVAPSFLSTQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEN5</accession>
    <name>A0ABQ5SEN5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012212</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Interacts with F-actin.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21220">
        <property type="entry name" value="CH_PLS_FIM_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF18">
        <property type="entry name" value="FI19014P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="756"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="983"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="336"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="594"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="658"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="704"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="337"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="600"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1127" mass="118110" checksum="B7D050089AE82EBA" modified="2025-10-08" version="1">MSILLALLRSPRISGPSRNPRAKTTQEFYTEMGVFNRCTTEGTELNELPAVAFPAPLASKDSAPTASNSLANELAGDESPLSLDFSYRASAAAEITFTYGCLNPGGCFVTDECVQEVFERLCWSGSILCNLLCSALPGSLDERAINTTDRGFGNLTPDEVLENFQLCYNCAHAQGCDVQELTVGRLCACEVSCLADFVLEVLRLSVVQVLPAADRQVILRPQFQAVGPATAGGAASQHKGRLAVTSLLPVGTHRQLPCGDCAPDLPIGDISSRSNSSSSGCADVSGNCDHRAGTDVLERCGLNSVTASPIGTAAARRAMAGLEYWALELVHTQPGGTALASPSLAGTSSPATPPPAPCGLGTSPAAHGPPLQLSPAERVTNPAAFSTIKVVSSHNDKWGTEVGWGPSSSGQSIEDGDRTYGAQVGAGTDSTIAATASNPVATDPAVTNEPDPLGLEIWEHLAALLSRPLACSAVPRDRTTFEHAQEMLKQGSPYSATSGDFSEGTPQPTIPTGLQVTVPRDATDDEEARWAAAAELLSKALGAAPNYGPPLPTLRVQHLQRRHPGLRALILARALLVHCRQRDRLLVELTPSRTNETAHGSNISNNSSSGSIYPTSTADVTPGSAAGKGAEQGSAAAVAAASVIAAAAAAAPKMLPASPAPPLPPRRESALPVSSKAVSRRQHSTGGHAPMDLLSEHNLLVQREHHQRELRRLQKQQQLSTSGGGNAGGDRPHATATETVKGVLWSGDGDDEEGTDQEERVLRLWLNSLDQSIHVTSLFEQEITTGWPLLIALEAIQPGCVPWSDAFRPPFKEKLRKILSVQNCNLVIDVCTRQLAMPPLVNIGGLDLALGQRRATLSLVFQMMRHHMGMLLGLAAPHPIPSPRCTSATAVATTAQQQPGCGLAHNYNLGYGFSRSYSLSCGFSHDTAPQLAQHVPYAPSSQRGSRAHVEVEKAVLAWANAKLQEAALTAGAAAAAAASNDAAEVTSAAVAWPGGVAAPQLPFTPLASFSDKRLAAGRLLLQLLAAICPRSVNPKYVLPGRDDEERGSNARYLLSCARKLGCVIFLGWEDVVAARPNLLLLLLASFMALDRKRASNAADAALATTTAIAVTGGTVVGLPTSLAPVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF35</accession>
    <name>A0ABQ5SF35_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012976</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13077" checksum="00A139A4D91E67FC" modified="2025-10-08" version="1" fragment="single">SLSAVCWEVVPLAKSLKFPTLERNASPLLHLVRYTPGMWRGRRVAVKVVTTSSAEEYDKVQHEVEVTSQLAQHPNIVTTFTSARVGPASRTASLVPERPDLHHKHGAAATISATAAAAATVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG91</accession>
    <name>A0ABQ5SG91_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Asparagine synthetase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013096</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF46">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparagine synthetase" evidence="3">
        <location>
            <begin position="53"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="252" mass="26688" checksum="5BB2B6C6768CDC1F" modified="2025-10-08" version="1">MLKAAITTASALGLYFLLWSRSSQRRRYLEVRKALGCALADISQDRRWDVNSACILLSGGLDSAIVAEVGGKQLGLMTAFTVTCSDDATDLPYAAASAAAAGLEHIVVRISLDELLHRYLPSLVRTIQSFDPMSLRNDLAISCVLHEAASRGFHCAATGDGADELLGGYGFTHGLEPAAWAAHRAHMARVMEFGSVRLGNHLGIFVASPFTHPAVKETALRLCKDDCVQVDGFLGADGGSGGAAAAGESWRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGH5</accession>
    <name>A0ABQ5SGH5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="131" mass="14145" checksum="FA318C99FFC71BE1" modified="2025-10-08" version="1">MGRTKRKGKSKKSEFDSFFDKKGGSDEGWASGISAMALDGDNTAIEPTLPAAGGSTGMEIGDAPRCQGPKGTALRVQKVSMKTVRGQRTKAQKLRKSKKIEKATARADKEVVRMGSRTTHKQTKNSLKAIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGI4</accession>
    <name>A0ABQ5SGI4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047285545" evidence="2">
        <location>
            <begin position="23"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="183" mass="19754" checksum="027D3AE117AFF633" modified="2025-10-08" version="1">MASTNLCSRMLLLSRLASHSQTCCSTLGVLTAPAARVANQMRDQATSIKAGGDAAKQKKKTGPREGPVEGNPRLQALVELLSSPTRQQIPLTKKEQDALAAHQQQVAAEASAWSYGMSVKFYLQQSALKALPPRLRELAMQTDHSPFPMNRTCLFDSPPESYRDPVPETNPVAKAGKGRAARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH24</accession>
    <name>A0ABQ5SH24_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="160" mass="17216" checksum="13BFE79236E4F382" modified="2025-10-08" version="1">MALNIHHRASTRCNGKVATLRPTRTVVTRVVPPRQEPQLGIANAMISTAAAAMLLMVPPAFSSESVSAPVAPAPTESAPTAPTNTERSSSVSSISFARVNISVTEKLRQRDENMAWKCKGVNMYDCDGELAAAAEKRFEEMANVFRGDSKQIGGYITRGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHA5</accession>
    <name>A0ABQ5SHA5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-exporting diphosphatase</fullName>
            <ecNumber evidence="2">7.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_013879</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.3.1" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01129">
        <property type="entry name" value="PPase_energized_pump"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001960">
        <property type="entry name" value="PRK00733.3-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01104">
        <property type="entry name" value="V_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001265">
        <property type="entry name" value="H+-PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="532"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="562"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI69301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="764" mass="79957" checksum="63D691E156F968D5" modified="2025-10-08" version="1">MAMPDSVVASLIPAAAIIGILFAIVLWKRVSAIQLTGGRLISSQNGREYLLEEQQGGGEEEVVAKAADIQKSISEGASSFLATEYYYLGIFMVIMAVVISSLLSIVTPEGDRTRKDEIRNGIFSTVAFLLGGATSILSGYLGMQIATYANARTAVEARKGIAPAFMCAFRSGAVMGFLLSGFGLLNLFLAITIFAKFYGDDWRGLFEAITGYGLGGSSIALFGRVGGGIYTKAADVGADLVGKVEKDIPEDDPRNPAVIADNVGDNVGDIAGMGADLFGSFAESTCAALVISAVSSLGESHDYAGMMFPLLVSATGIIVCLFTTFIATDLYPAKVISEIEHTLKMQLIISTLLMTPVALGVAVWALPSTFEMSVPSSSPDKAFDTKTVKNWYMFVCISAGLWGGLLVGLQTEYFTSNRYKPVQDVADACRTGPATDIIFGLALGYKSCIIPTIVIGVAIYVGNTLAGMYGIACCALGMLSTLATGLAIDAYGPISDNAGGIAEMAGMGEDIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAYVTRARITMIHSSILDPRVFAGLLLGAMLPYWFSAMTMKSVGKAALAMVHEVRRQFNTIAGLMEGTTRPDYKKCVAISTQAAISEMIAPGALVMLTPVIVGALFGTQCLAGVLAGSLVSGVQLAVSMSNTGGAWDNAKKYIEAGATEHARDLGGKGSDCHKAAVIGDTVGDPLKDTSGPSLNILIKLMAVESLVFAPFFYNCAHGQGLIFDFFGWTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHM8</accession>
    <name>A0ABQ5SHM8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="16100" checksum="969197A8B0764115" modified="2025-10-08" version="1">MDNTAGPRVLPLPQARGHDDIPEDPLGEAVALDQDFGATRPTPTAATRRSNRQHQARGGASSGVRGSGGAGPSSDPAKNRKRGGAPSTAAAQRSDPNAKRTRTDDTYMPKWNCNRSEFERRKLANVCLRCAESHSTKDCPLMPPFVPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI34</accession>
    <name>A0ABQ5SI34_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013683</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044644">
        <property type="entry name" value="DinF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42893">
        <property type="entry name" value="PROTEIN DETOXIFICATION 44, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42893:SF9">
        <property type="entry name" value="PROTEIN DETOXIFICATION 46, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="585"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="586"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="613"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="665" mass="69796" checksum="61434F5F8DC76531" modified="2025-10-08" version="1">MSAIGLYLPQSTSNGRFIFYISEYAPGLLRQELICGRGRTRPCDGRGHGQNRQKPTYRVCHSLRCRHIATASARPSIAIATDSEPAPDAPDVLRYQWDMLRRMISFLGPAILIPMGEPLMSLVDTVCIGQFAGTSQLAALGPANLVFSFCQYFLQSLQVATLSLLAGFMRDGRLRRGEELLSTAVAMAAVLGLVIACILESFPAAIITATGLRDPALLPLSTEYVRVRGLAQPAVLVTMVAQSGLLAQQDSLTPALIVAVAVALSLLGSVVFVAGLGWGLVGAAMTTVVCQYAGAVALLYGLSVRGKLRLHLALPRRDVLWQLLRTMGPLSITYICKNVSYLFIQTTAASLETTKLAAHQALFAVWNLLAWTVTPFEQAALTYLPGARGWRKAAGISLLVGLGAAGGVVCGAMLAALACAAPAVLTRDAAVWPHLQSVAGLASASMLALGADVVASGVNISMGDARYVAQSYLITLAALAAFMSASRTLGWELRGVWCGVVVFFGVRALQSTGRVLWQHLRPRAVERDDRRLQHLGRGDAEGSANSVSSCRISLASSDMDTAAAVPFVSVQTMESTEEAQFLACEDAQKPDRRDVLTSTPTEGAQGGLLDQRGTGPDAEGGAAAAPVGGERGNGVEVSNGAAELARGPVVEDAHGSGVSSGVAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI55</accession>
    <name>A0ABQ5SI55_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045357577" evidence="1">
        <location>
            <begin position="27"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="115" mass="12396" checksum="FB95672DEB5C92CF" modified="2025-10-08" version="1" fragment="single">MAKLLNLLTVTMWPVVVLTFLCVADAHKGASFGAQAHQQNVGVSGFRLSRLLNIWRPAMLKATSRDLPAVQDNLNLVSTLEAVFELPLAQQPSRCETGLPSGCVVELPLSVPKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIQ4</accession>
    <name>A0ABQ5SIQ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014560</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="HIGH-AFFINITY NITRATE TRANSPORTER 2.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="187"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="56200" checksum="19733FFB77FF4570" modified="2025-10-08" version="1" fragment="single">EEVQLQHNRTGSASLRGWSLLGHSDNCPATSQDLKSFASQSLTFSSGPQRVLRKEAATTAAAATATATASSASASAMEELNGVGAPGGTGSRNRGGSGGGGGESAGFRKAFLSPLPCSATQPIAEAYERNSAVHDPAALEMTVAPAAEDAVARGAHANRHLRHFAVVILASNRQYRDSAMIAASGAAATPTLPLRSPPSPSSLSMPTSSPSNRQAQSPSSVQNVASASASVSAVSMPERHPLAPSPLPLPPPAVHFIRTYDLARERTDEVTTYVEWHRSRMQQHSALGTSWLDAVHLFGIALRDYRCLLLSLNYGYNYGAQMALYNLLTLYLFQRYGMSFIAAGALAAMPGLLNAVMQIYGSCLSGLVGRSFGMRGRLWLLWASQSAGGLCCLLLGRCRGGSSGLTATALLLLLFALSTQIAAGATYAIMPYVSYRAFASVMGITSSGGQLGGVLLMLFFFVSRHMSYEQGLFWMGVTILVVSSTLLLLRFPTWGGMLTGAETPYGGFQKEDYYLSESTPAAQDSGPRGEGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJE5</accession>
    <name>A0ABQ5SJE5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014874</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="163" mass="18469" checksum="A45F3E3CBBC2474C" modified="2025-10-08" version="1" fragment="single">IHVLFSRELVAVKVQSPGLKQLFDTDLNNLCILAEQLALEVTEEVKATVLHPLDRTRRREFIHRFFVRLGRSASGTQHMEGGRRCSTQQTATTSTIVGSWRGLCSGRCAEWGCEGALSPRVPKPFVATPFVTPLLFSLFFTAPCYLRGFIHVIKYPYRTHIHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJF1</accession>
    <name>A0ABQ5SJF1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TRAF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014275</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="6">
        <location>
            <begin position="163"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="4">
        <location>
            <begin position="163"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="242"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="541"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="325"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="555"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="846" mass="88168" checksum="6C74E38DED7CECFF" modified="2025-10-08" version="1">MSEQDITTAIKKVQDELMCPICLDMTQLPVNFTCFRGCGGHFSNSSTCLRSTLCMHCANEALQLGLAPVARFRGTGGFAHCLICRDTRVDARMLTPDNAYAINHNVLGIMDALGMGAMCRQCDKDMGSQAALYQHYLRRCPDAVVKCRFRGCSHWHIRRGAGAHEDTCPVGRSQCGQCGTWIDRSELLRHMVTTCRLRVVNCVLCKHSALLPDMLEHLRLHQRQLQGRARARAVAGKAAAASGDAVVAESPASAGSEPTQAADMVGPKPLEPDRGGATTSGIASAPSASTPILAPAAMAAPQQAGQSRTFGAAAAEHQPEPERPLQPQSSSLLQQPQQSAWAPRGPLAQQELSYLSRQAAPQLLEILRRQQHLLQRLQTQRQTLTSGLVVQQQEQEQQQQQEQAQRQPGVSPWQADRTNQPDTLAAAADIGQVTQAASGGDGCGGANLAVTPVVPTPPLCPEVAGVATVRPGAAAATAPDAVVVERVEGSGADIIAPQMMIVDPLLAAPEQHGLRYDGADALATVTATNRASRGISSFAIATEGDGTPVGDDIARSTGGSLDSGDGSSQRSLDMAAMQQDRASQPATDRNPWLGIYSTSSGGADSSSSNLFADTALHRTPSWRTGLGGPAALISRRGGVGPRYLSDVFRSGNDGGGDQDEYAFASELSSEFVSAAAADEAVRMVRTAVAIVRASAAASERRSQRQQLALASSAASGSGGAANSGGGLGVQASAGGGGGTMAAAWWGVACPGAFGRSHAAAAAALAEMEQADRSTSSSWTPAVAAIEISPQDYPSAAAGPLQKKPLANGIRRSFSDTELVFDVLEELVRASTSSGEHRAVRISASGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJZ8</accession>
    <name>A0ABQ5SJZ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015072</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044">
        <property type="entry name" value="GLUCOMANNAN 4-BETA-MANNOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044:SF80">
        <property type="entry name" value="XYLOGLUCAN GLYCOSYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="7">
        <location>
            <begin position="2"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="174" mass="20148" checksum="055DAED88BB5A53E" modified="2025-10-08" version="1" fragment="single">DAGGWQSDTVVEDMDLSLRCYLKGWNAIYLPHVDNPNELPCTLSSYKTQQFRWLSGPMQILTKSFSNIMRARDISIGRRLNAFWFFMRYILFAAITVGVLAVPPVALWVEDWDWSWPQIYFIVSINFALAVYLYVTPFSIAYLFFSVAIGYFKLWAMISGLLGLEKSKTWKVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLF8</accession>
    <name>A0ABQ5SLF8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UPF3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RENT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12455">
        <property type="entry name" value="RRM_like_Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039722">
        <property type="entry name" value="Upf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112:SF0">
        <property type="entry name" value="FI21285P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112">
        <property type="entry name" value="UPF3 REGULATOR OF NONSENSE TRANSCRIPTS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF3" evidence="6">
        <location>
            <begin position="7"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="238"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="305"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="403"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="407"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="424"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="494"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="558"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="598"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="617" mass="62772" checksum="CF13F0E48CE53EB8" modified="2025-10-08" version="1">MITARPKTKVVIRNLPPGMTEETFMTVLDNLTGGHYTWLSYFPGKVRLKRVVFSRAYINFSTSEDVFDFKQRFDGHVFIGQKGHQYRCSVEYAPLQKVPVLEEKPHPLEGTIEKDAEYLAFWEAYESGQAFPSTAAPSTTAAEMPSAAASSNADGGEGKVVMSRLMEFLVRKYTEGGNRLGGMRAASKAKQSKQDLIQQVQVSAQEARGTSGGKAAKVAAAAAAAAATAAITAAATADTTVAAKGSRRERDRSAKGAPAEVVNPKQAERQSAASRGATGKGARSSTVEASLEAAAPAITLLTRATASKGQCKEPAPEKSSGRSGGGGRSDAQYSQQTGSRVSGTEVETGAALAQEGGKARNGVAGRGTAAQQGAARGAVAHEGTPLVVRPVSTSTPRLLLIKGGDARQTQEVSSSGQPGPAQSTTAAPVTPVATAKKSVQEQQGARQQPQQQQQQSQARGSKQPSTSTQQRQEDTNRAARGAAAPVATQHVKPSKAQAPASAAANRSSSKASTASSTAAIATAAGSKGTAEAPAVTTAIVSTGSVPEPTDAPAAEDGLAATASAAATAALTAATARPSGKKVRAGFQMYVPGSLRGRAPGGTTGTGGGPEGGSSATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLJ2</accession>
    <name>A0ABQ5SLJ2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35748">
        <property type="entry name" value="OS05G0358400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35748:SF1">
        <property type="entry name" value="OS05G0358400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="443" mass="48495" checksum="583266EAA2C08A9E" modified="2025-10-08" version="1">MSISLKSNPYGLFNSRSLSAICLHLCKKGQGYSFLKHLRLKYSSAASVSAAGRTRAMSRQVVNTSAGPQQDLVYDDELDDQFTGLVGDDGLMTVAGFGSLLSGNIAYLATLTVRKQIEMLASKSQACNVEPWTKHQRLGQGETCGSGHKNPLVTLYLRAHVQGWRRVFAHTADIFFARGIARPETGEISSLSLERVPPTLAEDEEDEDESSTSSAPVREAATEAVAVTTGEQPLYGSSQLLLSADDIDANAGAAPTRSPSWSLDRITPPPPPPPPPPPPVAAAEADDSRHQRRRRRCSVVVSLFEVPYNPANVAAFIAREHEFRFVAVEPYDLNGVPIGRKAVVCARNTDAAYRAARCPPDEWARRWAVHRISSVWRDDVLPCRVYLRHCVLAARSLGPEAEDSFLNDTYLADRSTTIGEYLLLHPDIMDEQPPLALAERYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM77</accession>
    <name>A0ABQ5SM77_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015761</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="170" mass="17706" checksum="2C55034B51B34E78" modified="2025-10-08" version="1" fragment="single">YCFTLLTTLCNHACCNVDLKKIEFNVYESCLVSGASVSATINGLPTSVLPSYDRPQYGPPGSAILRVTQLGLNTSHNLDQICITLKPNRAGLGCTTLEQMCVPPAGMGPGSCLAAMFDYSLNCCPTVPIGYMQPPPPSPSPPPPSPPPPPPPSPPLPPPPSPPPPPPSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM97</accession>
    <name>A0ABQ5SM97_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Elongation factor 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Eukaryotic elongation factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">VaNZ11_016208</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000102">
        <property type="protein sequence ID" value="GLI71115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18626">
        <property type="entry name" value="CD_eEF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015688">
        <property type="entry name" value="eEF3_ABC2_chromodomain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047036">
        <property type="entry name" value="EF3_4HB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF5">
        <property type="entry name" value="ELONGATION FACTOR 3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <feature type="repeat" description="HEAT" evidence="15">
        <location>
            <begin position="103"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="15">
        <location>
            <begin position="183"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="429"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="688"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="17">
        <location>
            <begin position="819"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="783"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1018"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1019"/>
            <end position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GLI71115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1053" mass="115131" checksum="A6C710D819755353" modified="2025-10-08" version="1">MSADADVNLVIRIEEVSLKVAELPESKASAEIIAKIASGDAAAASDLATHLKAAGAKGFGATQALKAAIEDKANANARVVGLAAYQAICENIGSAAEPFLAPLLPAVLEQCGDKKPEVKAAAEAAAKALINIINPHAVYTVLGYLFECMDSKKHPATKEAALNLVKDLVSMHPKQITRALPDIVPAVSGCMNDSKQSVKDVASECMKAACTLVGNRDIEAMVPLIIRSINSPQEVQDTVHKLAATTFVQQVEAPALALMVPLLLRGLRERVTAIKRKAALIADNMSKLVDNPAEAMVFLPRLMPEIEKVAAEAADPELRKVATAAQKTLQRIEQEGKEKLSKTLDKSAALKSLHDLLAASPDGKKGLVPEAYPVLDYAAALCANLTNNKNFEVEEWRDKVLGTYLGVFVSPSTLAPIAKTLCDKCFAEVQVKSTEYFDDEEGDELCNCEFSLAYGAKILLNNAALRLKRGRRYGLCGPNGVGKSTLMRAIANGQVDGFPPKDVLRTVYVEHDIDGSLSDLSCVEFVFADADLQAAVATTKDQVTSMLSSVGFDEAMLAKAVGSLSGGWKMKLALARCMLMKPDILLLDEPTNHLDVRNVAWLENYLVNLRDVSAIIVSHDSGFLDHVCTHIIDYNNRKLRVYKGNLSEFVKAKPEAKAYYELTASTLTFKLPEPGYLEGVKTKDKAILKADKVAYKYPGTDRLIFENATAYCTLSSRVACLGPNGAGKSTLIKVLTGEVEPTAGTVWKHPNLRIAYVAQHAFHHLEKHLDKTPNEYIQWRFAPGEDREAQEKETRLTDEEQKKLQETKVQIGDDPKVKRSVEKLLARRKLKKSYEYEVQWANTPADQTSWLPRDTLVELGYEKLVNEIDIKEAAAAGLFTRPLTTASIQKHLDALGLESEFALHSQIRGLSGGQKVKVVLAAATWQNPHLIVLDEPTNYLDRDSLGALAGAIKEFGGGIVIISHHNEFVSALCNERWIVGNGKMVREGENLIAFKEKVEIKVQDEVIDAFGNTIKVKGPKKEKMSNKEKKALEKLRKARRERGEVVTDDEDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN30</accession>
    <name>A0ABQ5SN30_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_016036</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1057"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="378"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="702"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="825"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="882"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1108"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1351"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1493"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="703"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="882"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="921"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1124"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1148"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1358"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1493"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1516"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="1084"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1527" mass="156555" checksum="7A3E0B789F206E26" modified="2025-10-08" version="1">MPLNCFACFGGSPKPADVPFVSPIEEKGVEHVNFEAQVRDDTFLQDFQASLSGAPTDSAEARISQVAALVLRHLHAKVVRLYGWSTDDVDEGTAGHLVLLAVDWKRFTGTGATAAAAGAKNGPSPPALQELPDIPGQRIAVPIKPPIICSISPGTIQAPLLQQAVHNKSPAVFTTPPRTAPTPSTMPPDCASDHAALGASSFAALPLTSGDRLLGILWIGAASGVKSEPAAPHEGLPPLPLLRQVAGSVSMCLNGGVDPEYLAWLTGSLRRLAAAATLQAFIGELCDATAQHVRRRFLVDAVAHAAVVPEPRSTVGFMLDHRPQPGGPGGVARWAAASRQASVTFPSAAGAVANGVGPGGSGAPRGALVSGQSIRRSDSFTSQLGGVRPQPARTSSHVPMRPYASTSKRSLMEQAAARSAAAAAAAATASAASAASASANANANANAISSAASMGNAEAVRAIPVTGGGGRITPGGNQASDAAVAAAVVAAAGAVPSLRAKGFQLSHTLLQRMVAAAEGRGQDGVYVGLVVPDCGRHVQDVHQPSRDVCMLMAGGVRGADMTADASDSNMFASERSTGRIAMQSLVLVGVDVGHVGRQQQQLAESSASGVAAATAASGGAAGGGGGGGGGGGASATPGAVLALYLAFPTRLPPLLVASAHTSCRQLLGLMLARMVRVKIATELDVEFQTLCAGVPGSYAVVPSTPPAPPPGSRPDPHGAGGATPGSVAGAVGAGGANGHHAGAAGVSNFAAAAASPLDASGLRSVALEQSIGSIARASSCKLVPLNGMPGLTGGVPGPGMSLGASAQLLAAFTDAGCRPVSMQGSVGRVDAPPRPGLQAPEASGRVFPQTHDGSARTHGGSAMQPPGNVISLCQDRLFERTAEEEEAAAEEEDASPTHEEADVMLSSDPRVDDADEDANEPNDPNNNNNNSAAARRTTTTRCTTASSATAAVTGAAARRTGSTTAYNSSAAVREEEDDDIFPSDLPDYPAIAPRVPPTPAAVLTVSEADGTSSMRQHMGLLVESLMSTLRSTAQIDDHFDSAVVSARRSMDLELEDLRLSGVLGDGGSGVVLCGMLGTVPVAVKIIQMPEVDQLLLASTVKVLGQEGSKPAAQQHYSNHVSVKGGSPSSPRHGGGDAAQKGQQAATPHGGGGGGKGGDGGGGGAAPAGRASLSSSKLKQARRDMLRNAMELAVMRSISHVNITTVYAVYDNVILERVKRDGGSTAYALRRQCPEDMQLDKTGSKPVFVALCMELCDSGSLASKLEERSFPRVYIREEEGRTKGARVLDMVGIYLTVLEVACALRYLHARRLLHRDIKSANILLRSSPTDPRGWTCKLADFGSALVLDQFQPPEDMPGGSPGEEGGMGLLEGGGGGGTAGRWYAIQEQSWGTITHMSPESMDNNSRVDASSDIFALGIVMWEIASGRGYRPYKELAPEQIGAAVRRGLRPTFTSEVPLPYRTLAQQCWATEPQKRPSAVQVVAQIKAQLSQLQSEVRFQQQQQHSNNNPTPTLPRGGNPPVPNPKHEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNF3</accession>
    <name>A0ABQ5SNF3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="32368" checksum="F4EA7479C9BA7314" modified="2025-10-08" version="1">MLYLTSFSHALDLHVWVLDYSATAALLARFTLGDQSWIAAVPRVLAFDTYRMQLNETVDAYQSRFEALIAPVVDLTERDYIYWFQRGISDTLARESTADLMSAGVQSYVNWVQIDRNAETRILTKQEPSRFTTPVSATPMQNVVCETEPMYGTTSVPLSPATMDPNGTCHANNAPSLETQGSAHKDHEITLARRKREEEPRSTVGVCGTDPEVKRQQVQDKWLTKWECMLAELTWRQQARVCLRCASGHPTRICPLLPPLILKQEATGDTQPAASCEAARMLSQVQEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP45</accession>
    <name>A0ABQ5SP45_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016364</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058917">
        <property type="entry name" value="RESC6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26188">
        <property type="entry name" value="RESC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAP" evidence="2">
        <location>
            <begin position="1227"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="701"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="950"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="320"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="955"/>
            <end position="976"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1313" mass="140958" checksum="8C3732C324B5EF3D" modified="2025-10-08" version="1">MLRTGYLSLSFSIAERRHVCHESSSSSSSRVTEAAAPQSRRHAPTLPGPQSSKASHLISVDLPKTGSGSGDNISSEHGREIRSFANGRRSNSRTRSSKRGQTSGADGSGNVVEDRVACASGGTNCGPTSGDVASSSSSSSSCNSLEGDRRNTDHDGGRHRASRAFGRGRGKRDIIGAYRPRSAAPIADSMALARQINRAIVEAGSSRNCQDILQIVDCQGGNFSAVNVATALHRLGSCGLQPGSWEAKRVLTDPRFSRLMETLAEQHLTSLSGPELANCLWALAKLGYNHPQRGAGAGSGSLSSSVSGSGSRHDGGRVMESSGVTGAGSSASGGERRRKGSRGVSNSSSGGSGIHLLQGLSVRMEVVLGQLSSQAVAKTLWALGRLGHHPGEHLLRGLTNRLLKVLADASSQDVSNSLLGLAKLKWSPGRAVLDQVAHGSLPRIIAESNAQELANTLWSMAHLNYSNNQLQAAIFHQALERLATFNTQNISNLVWAAATLGLGPDLGPNPSGDDIGGSSGRDGGGRGGIIFKQQKQGLIPEVLLQKCCDRAAMILLEGFAPQELSNLLWATAKMGYRHQALLTAAADQAARQLAAGAGMGAACPSGQQHQHQHVQPICWPSQAVANTTWAYATLGAQPGEKYLLQVCRQLSSPQLLPGYKWQEMANTVWALATLQYRDCTAALAAVEREVLARLGGGPATDLANVDHNNSTGGQHQQHNRPTQQRHNNSIRHSRRLLESQPELRPQHVSNLLWSYATLEHPAPDLFTTFLPLLERRLPLFSEQEISNSVWATARLQVYDEKIMDAVADHICSHRLPYLKPQEVANIAWAYGGLRHEHKALFDGLVECMVDFIRMPAAAAAPVADSTVAAAAASYTALPAAETDFEIDTDVDTEIDTRHTHTNITRTAVKIQELSSICGAVASFGWYYPVLLVATKQWLMRAAGTFPRSIHRQRQQELQQQEQQGDQSGSSSCCGTGSPSTGITIFHHQRQEGATAGAVVALEAAPPGGAGQLAEAAAGQGREIGSAQQQLLTSTDECYSRPYRQQQPHQPHQQLVKARDVCNLTWALSLVGGCSPGMWAQLMELMGRVVEERGGSGFHELLDEELSQMYQAYLYVQLDYPTAALAPGPPGLLSEGLQAWRWQSLQGPLVSSFQMEVSRALNRLGLSHTVELLTNDGKFSIDLAVEVEVDVDMEVLDLLIRNAAGVASQARGSSFSHITSANRRLVRVAVEADGPWHFTANTRQPLSPTLYRRRCLEDRGWVVVSVPYWRWNQCRTSTSDGATSSGPADQEEALLLQLMREEGLGEVIEAVAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPF1</accession>
    <name>A0ABQ5SPF1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_016742</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008854">
        <property type="entry name" value="TPMT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32183">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32183:SF11">
        <property type="entry name" value="THIOL METHYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05724">
        <property type="entry name" value="TPMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51585">
        <property type="entry name" value="SAM_MT_TPMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI71516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="29881" checksum="4DFBE3B91365DD31" modified="2025-10-08" version="1">MLLARRIVFATLRPNRSQSLVIDVQHKLLTPRMTTVVDSVSSPPSGGASKAPEPPEPEVYSDRWEMIWGAGLARGERWDAAAASPAVVDLLRSGKIDVKGARVLVPGCGRGYDLPVFVSSGAREVVGLELAPTAVGEARAYLQEMNAAEGAPPGDMRVEEGDFFLWTDPRGGDGSWDVGFDYTFGCAMHPSARQQWGHHWGRHVRSGGVLVTLVFPVNSEADPNVGPPFPVSPELYSEILVPQGFEVTYNEPVPRELSHPSRAGREVMMVWRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPK0</accession>
    <name>A0ABQ5SPK0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_016666</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF169">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SBP-type" evidence="5">
        <location>
            <begin position="49"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="224"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="237"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI71450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="388" mass="41774" checksum="A0D9618FD3D10986" modified="2025-10-08" version="1" fragment="single">MTEPGTTPPELSIVGNQAGISRRRGRSNRRRDAADAQSARNRHARCANLRTCKVDGCTRVMESEKAYYRRYSVCEQHLKSSGLFVDGKVQRFCQQCAKFQPVGDFDGKKKSCRIQLAKHSARRRDKLRSERLARRQREREALLAAVEGVGTNVDGGNTANSNGCGAANVEMCREIITAVVSDVGVVRAPPGGADAIEISGHPAHAPTTTPLRLRQPPIATARRCSAIQELSSSCSSTEEDEEMDMDISDDDDEDFDDQDEEEEEMEKERTAVRHVASQAGKSTALRRRRPSSGEEREGSHGKTSRTDVAEECDGRGGSSKKSGGSDTLECLPGVTSALLMHNKGYGGVGAGAMSLEAVQILNPLTSSPAASSGGGTFNRSPLPAVPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPS3</accession>
    <name>A0ABQ5SPS3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH:ubiquinone oxidoreductase intermediate-associated protein 30 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013857">
        <property type="entry name" value="NADH-UbQ_OxRdtase-assoc_prot30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020">
        <property type="entry name" value="FLAVIN REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020:SF50">
        <property type="entry name" value="UPF0659 PROTEIN YMR090W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08547">
        <property type="entry name" value="CIA30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="114"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30" evidence="1">
        <location>
            <begin position="251"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="435"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="65514" checksum="14F477FD9B5020FC" modified="2025-10-08" version="1">MFSHGSTSGLASAARPRPCPSRVASWRAHRGRAIACADPSNGGSGRQPWDIFRFLKTVAYFNELPTLDKVLGNFLKQLTPQSQGDSMVSVDLPQAVLSASAAVVPLDGIIMVTGATGGVGRRVVARLLAAGKHVRALVRDLDKAKTMLSGLPVAAGGKLELAVADVVQRQTLLPEMFEGIRAVVSCAAVKVSPKEGDTADRAKYYQGIKFYDPEVVGDTAEAVEYNGMVNVLDAASDSLGIAGGVKLLHQSGQSAAEWGPLDDVVMGGVSSSGLELVAGRGEDGWAAWVFSGTVSTDNFGGFASVRTRNLDPPLDLSPYEGIALRLFGDGQRYKFIIRPDANWDGIAYCCSFDTQPGVWQTIRIPFVDFFPVFRAKRVAAGSGAQPLDPSAISSIQIMLSKFEYDGQLNPNFRRGPFNLPISHISTYLPADLPPRFVHVSSAGVTRPNRPGINVDQEPPAVKLNDALGGILTWKLAGEDALRASGVPYAIVRPTALTEEPGGMPIELDQGDTIKGKISREDVADLCVALLSCPTVADITFEVSSRVPFSQAWALPDPARPPPPRDWAAALSAAKIRHGITGRTVAGVYTGRQPEAEALQAAGIIASARSTKPGPSPTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQU3</accession>
    <name>A0ABQ5SQU3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone chaperone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_017085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000120">
        <property type="protein sequence ID" value="GLI71764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14037" checksum="40F21CB474235D83" modified="2025-10-08" version="1">MTRNSSRKARNSSKAAAKKVTNDDDVRPSTKVRRPSTGTKRKVPLEDKSMDDDEIYERQKVKSVSEGVRCTRALRQRGSSGSKQKQELLEEEVSEDEKIEDEQDDATSAPEEDENRESEIEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMB0</accession>
    <name>A0ABQ5RMB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_000455</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex with RFC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
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        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 5"/>
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    <sequence length="380" mass="42451" checksum="DE4ADCCE445B16A7" modified="2025-10-08" version="1">MEIDRQDVTAMTKLDEALTAPSSRLRTDGTVDGRDAPWVEKYRPKSLDEVAAHKQIIDTIKRLTSENRLPHLLLYGPPGTGKTSTILAVARQIYGNSLANMTLELNASDERGINVVRQEIQDFASTRTIFSNKFKLIILDECDAMTNDAQFALRRVIEKYTRNARFCLICNYVSKVIPALQSRCTKFRFAPLDPQFVRDRLQYVADTERVKLGRGGLDAVVELGNGDMRRSLNILQSCHMAFEMVDEQAVYMCTGNPMPSDISQVLTWLLNDAVADAFEIIDVSVQRLLTRPQSSASSSLCITEVTKLQVGKGVALVDVVRELHPWIMKTNMPVHAKIALVDRLADVEHRLAFSTSEKLQLGSLIAAFVKAREIIAKAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMD1</accession>
    <name>A0ABQ5RMD1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000497</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="DUF629"/>
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        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
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        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DUF629"/>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="545"/>
            <end position="566"/>
        </location>
    </feature>
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    <evidence type="ECO:0000256" key="1">
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        </source>
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        </source>
    </evidence>
    <sequence length="3391" mass="353853" checksum="3255465EFD526345" modified="2025-10-08" version="1">MSTGQGGGHLDQSFFRIATECAGVLKDFCRGQRAKAKKALQRLAQEHPNSFLPHRYLARLLYHESARVEADQQVSSGTLPSHRRLALLHSALLEAKQASELAPGSLSSAALRATLLVNLLVEESALKGGLAIPQEQPPHSDERVASDACSELTGPPAGHGLGDVAWFQLQPEARCEAIKREFREAIAHCVRTLECNAPLLVEPVISISDAHARTCDPCCLRVQDSIVEWVKQDNWQRIVAEKRSVLACMHQVLESCHTLLDSTHIPVDGIVRLLQHILRPHQQDLQLWASQLLLSKGSVDDLQHKFETQAAVTELLRMLRPGRPNEELAGSIGTPQPGLLQPPQGKAQLSPAVVQMYAPANAAQQQPQQQQQQQQQQLSASNNAGQAAAAPPDAIMGGRAVAGPASSQPLPPPPQQELAACLSAEQEWDDVRDGGGVPSAGAGTTGAGGGAGIRRVGGKGKNKVKGNDAARGARKERSRYERYMDVETFWRTTPPEQRRELLKVPMAALLKGVRREHGNDAVDELIEGLVLLREQGNRAACYWLCPVCEQKFHSSRDFLGHVEMVHEGLAVQDNKYVCCYKCQQDVVGMYYTSTRTPGYNLCFRCYSADAASVSTPEAFEKVFMRHSAGRPWSSHSDFMSTRGSFSSLSERSHRHHHQQQQQQALGTQKLHQPGGSTGVGGAGHVHSAACRHHPHCPHHSHGQSHGQQQYGLESSSTQPPLKLGEASDEWLAPFPAASLSAPLMVPDPRAGLQGTSGAATLPPPHAPGMRPHGKRSSGGSEVSSDSILGQSLHSRSLSEPANHGLQAGDQGQGAGGSGITVIDDGTDGGGTGSSTGTWRSWWPTRLMQGWAGKPQQQQQQLSGQQQQGPGQQQQQQLQTAGSGLSATSRENSCQGAAQPVFDHGGLPDAPRLGAKVRSSPNGAGDSDSAEDEDGAKPLLQAAGGTPARVGYVPGAAELTGIDGFGRMNGMVTELLTRLREIYLTDRDLGDTTLGYITQFVYRKLGVGAAADDLHLASCPPGPRTVLLEFLAQPSVVFQRPQALAVVLSLLPLVDLQIVAAYAVRQHENAVSGMEAAARDIVAGSDGDADGRPEEEEDDCDGAGAGGGGSGSEQEYQLGLAARREAARVAAAETAVASQLAAPIPRARRRLRYTSRRVPAASSAASQASTGIQSSSVSTQLTAPSSQPQHHFPSTRPTTPPLPPHAGGGFHIGGSHALTLRQVDDDVSAEEVSEEEMESTVDGVEYALATDADASPLRCLSTQHAVAANTMPRSQQHQLEQLRHAGGAGMHLMALPGTEVANDILGEDDRSVEGTLDEEGDEEVSEDIVGEADEELSEEDEEDGVSLVPDEEEEEDGPCVSLFNVAGDVELGFDRLITQMWGGGAENDEAASAVLDQQAAAANSVPASCAGNNNYARSRSQQQGNRGGRNHNQGRQQSPAARGLGMGNNAATVATGCTLTGVLGATGAVGLPDDLVLAEELVGPREDEPYLLVADWWLHHLRLDGGGPVPDELRVLRWVYGNVINVQAEEFCARQRELRAGRDRDASILDLYDEVAAVWRSLQAVTDKRSRLEALRRAARGHFAVVRRLEEAGGHASLEQATEFLDAVLSGYCQGQQGVVATAASSTSAGAGTGDGAPVGPPEQPGTPTTSEATVAPADAPATGSSAPADLATASAAAGGGNPTVATGAGGASSGPSGPLLYLCERAVEVVRRHPALAQEPSQRYAAALLERELSVLALVEVMVAAEAEDAARERGVAEESLKRHRAEHREAEAEYQRVQAEGPASHRKKDLLDKATKEAEHREQLRELAARLASLSDNIAADEANKTRYAEKQEEAERELAYVRDAIRQTSTRRASLLDVCATLEVPFAPPPQHQQRANLPQQRLNRAGGGLGAVQADHVSVDGSVATPSPSRAEGDVCSAALALGPPSGGGLGSAAADMEWRATRLECLMYRVLWVSEAVKMFQPQYNPRPTTHHYNLFIRATHWAKQLSEEYEESIRAYCCELERLRARLRDVAAVDLGYEIGAAALEVIRRRIEAAARAAREAASLTLLKELEEEEERRKAALTAAKDAKDSGGGAGGGSSKKAGANKKAAKASQDSRSRAQKEKERELARQAEEEARRRAEEEDKERVAAARRAREAALEAELERRRRELEELEAQREAEAIRAAQEASLREQQLREQCERDRREREQREKEQRGTEQREKAIATAGLQQTQAQVQQAQLQQHVAANAAASATTLRSGIAQTVSGMAVSGARKPGGAHLQQMVQPHQQMRRHATSSEHSPEKSSPPPPPPPPPPPQHQHQPKRNAPNGTRARVATTALPDRLLGGTPESLNELRVPVGMQLANGFQPGASSRGGLPTKAGAAANPASPPQSGTNGATNMGLTGGKARQLATAHVSPPASSSSSPAAPDSAAAAAVAAATAGIVNKATQIAVTTPGGARSSPAKQCPGGVPSTPPAGGNIARRHQQQHGQSSTQAMNPAGGPNGATLTMPLAAASSGPAWSTAAQATTHTVRVPLQLSHPQQPVPQTPAPQLDAQPGQASGLPGMIQGPLSPGSEFPPLSAAVAVATAGHRQAPAIAPAQPVAQPPQQTAPAPPPQQLKSFAASAPMQAHQLQAQVQAQTHAHSHVKMQAPNQAQAHAQGFAEAQARLMAFAQGPGAAPAAAMGVAQAPLQLHGELAMLHAEHLHVHYCQQQQLHQNMEHHYRQQLRPQQQSPSPSPQQQSQHQLLHQQHHHHQQSASAQQQQQTLPQQQAQSAPTPLQVLQSSPCRPPVDGVTVPDFNPFAENPVFKALAMDAKAPQPASPTSAAPLAAAATPGPTPQVQQQQAPAQAPQPLPPAQTHADALLAIQASQPGSTGPEADTHIQSIMSILDQDGQAPPVGSGQNLCVSQHGLGPGAKASPTGTSASTQSGATSQTPTASSSPQRVLMPAAPPPGPVTGLYGNPYGISSSGVGPPLLPPAVDGQPGAATGIGPMAVMSSSAAAVRMQTTVQPNGASRGLGGVGANGIAPAAMGTQADALATSIAGAQPGVLAGLPASAAFGSAIWRAGGEQLGPGSPLPVSMLGLGLGLSGIGSGPNTWSNMGSSNPPAGGGAANPRASAGAMTSPRQASKAGTVEWSASSVGVGPAAVSAVVAGAGRDGGLTLPDSVDTLVASLPTTLLPASLDAEPAPSPIPPPGVTAAAAASGLAPTAAPFYPASLRSKVGAVGGVGVGANSGIIQAPQQQLVGCAGGPGGIAMGPTLPGDSSAWMNGLVPIPGKQGAAAAAATVAAANGLVVQGQHVTQQHLSGPGGMAALPGVLGPGGMRYMGLYNGDGVNWSHGGIMPAAGGMVSAPGIPAAVRATSSTWAPFVPPAGGMAPSQQQFVQQAAQQQAQQQPQQMAGWPRGQRWRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMR8</accession>
    <name>A0ABQ5RMR8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="71"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="320" mass="35207" checksum="CF473CD082DC3DEC" modified="2025-10-08" version="1" fragment="single">LCAQSAIEYLGPHGSKRVYANKLGIDICQYFWPAAPSTTPKGILVLAHGHGGYLQFDCLRSQGPGKPCVYEGSFVQQLNVAGYAVCGNDDRGAGRSGGLRGYCDSFNDYVSDLLDVARCCTSLGISSFEEGLPKFVCGLSKGGCVVLTAALEALDLFAGVICLAPMVSLEKVAKRGLNPYLRPLGKLLSRLAPQWPLFSTNRNTMYPDLQHAYDVDENCYHGNTRVRNAQEYLEATERLAANQAKLRLPLLFFHAKGDTMTDPEGTLRLYEAAESSDKTFISPPNMWHVLLKEPGHELVKKQILEWLDQRTVPRATSDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNI2</accession>
    <name>A0ABQ5RNI2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-haem dioxygenase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000949</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48420">
        <property type="entry name" value="NON-HAEM DIOXYGENASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48420:SF1">
        <property type="entry name" value="NON-HAEM DIOXYGENASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="54980" checksum="3EB7F0FC74FACEF4" modified="2025-10-08" version="1">MIAIHAAYSPGILFGRRASLHQVPGLTARRIIVMRKASTEDKASGGHSEATVKAGKSVVPAASQLVCIEFKDLVAQKDLTYELEMALGPNGLGAIVIKGVPSYSKLRGALLPLAERIASLPPEVQEKYVDADSSYSFGWSHGKESLANGVLDMFKGSFYANPLVDDQLQPGNGDWLQPWAFPIPADEANPTDNQLPSGTAMPATPYDDTGMPASSTASNCGGYLGIRSDLEVSELRRMYPEYFHKNLWPREEAPELEIAFKDLGRLICAVGCLLAEHCDRYVSLKLGTSPGLVSGVLRRSLNPKARLLHYFAPSETPLAGCEANQQQQQCHPMQQQQQKASGGAAGISSVTGDKEDEQQAWCGLHTDHGSLTGLTAAMYLDGQGREVPSPDPEAGLYIRDRSGVFVRAGIPPDCIAFQVGEALQVHSGGLLMATPHYVRAPRSQLAQGISRNTFAVFMQPDVLERMDCPPGADPEHVAVGQWKPGHTFGEFAAATFQQYYPKQQQQQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNT9</accession>
    <name>A0ABQ5RNT9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033362">
        <property type="entry name" value="SSNA1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28661:SF1">
        <property type="entry name" value="MICROTUBULE NUCLEATION FACTOR SSNA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28661">
        <property type="entry name" value="SJOEGREN SYNDROME NUCLEAR AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="130" mass="15031" checksum="6353B4623E3B4E64" modified="2025-10-08" version="1">MCLPYFKLANCFCWRLRTQMSAQGTALQNHNNELVKCIEDLREKREEIIKQLREDDAEKAKITQDLQILTKRLAQVNDSIARKTETKNEYDKVISETEAAYLKILESSQTLLTVLKREAVNIAKKKQASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNV9</accession>
    <name>A0ABQ5RNV9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052050">
        <property type="entry name" value="SecEffector_AnkRepeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46586:SF3">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15529" checksum="67EF937DD0385234" modified="2025-10-08" version="1" fragment="single">QEVIHSMRCAAIIGHLPVLELFFARGAPINLEVLQEALSGGQLRTAQWIWERLAVDPEHLTPYLFTAGAASGDLEVLQWMYDRGFQWDADTFVMAAEAGSEEQLEWLAARGCPMGVDGQAYALAAGRGDLPILRCLRRLGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP83</accession>
    <name>A0ABQ5RP83_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Chorismate mutase</fullName>
            <ecNumber evidence="4 9">5.4.99.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_001238</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.5" evidence="4 9"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.590.10">
        <property type="entry name" value="Chorismate mutase, AroQ class superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008238">
        <property type="entry name" value="Chorismate_mutase_AroQ_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037039">
        <property type="entry name" value="CM_AroQ_sf_eucaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01802">
        <property type="entry name" value="CM_pl-yst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145:SF12">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017318">
        <property type="entry name" value="Chor_mut_AroQ_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51169">
        <property type="entry name" value="CHORISMATE_MUT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="10">
        <location>
            <begin position="179"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI59373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="330" mass="36450" checksum="3FE181164F756C2A" modified="2025-10-08" version="1">MMQTSQRNLWARAPNGHRNARRCCSTTKKAITIVSAAANARDMSSALSLANIRSSLIRQEDTIIFNFIERAQFSRNLPVYTPDAIPVPGFDRCGRRYSLLEYVLRETEQLHGSVRRYTSPDEHAFFPDDLPPLVLPPITYPSVLHNCAQSININERILRIYMNDILPGLTAPGDDFNYGSSATLDVTCLQALSKRIHYGKFVAEAKFLAKPEEYSALIRSRDAAGIMELLTDREVERRVVERVRRKAATFGQDITENGAVSVGTGANGGTPEGGPRLKVSPDLVARVYEDIVMPLTKDVEVMYLLSRLDGVPTTTVAVEASQPAAAAPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPA2</accession>
    <name>A0ABQ5RPA2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Clathrin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_001245</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasmic vesicle membrane</location>
            <topology evidence="2 7">Peripheral membrane protein</topology>
            <orientation evidence="2 7">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <location evidence="7">Coated pit</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="7">Cytoplasmic face of coated pits and vesicles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the clathrin light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000996">
        <property type="entry name" value="Clathrin_L-chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639:SF7">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01086">
        <property type="entry name" value="Clathrin_lg_ch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="6 7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="225" mass="24423" checksum="6940F3BDB1A07DDA" modified="2025-10-08" version="1">MEFEDSQFQTAPLGGGSGSFAAASVPYEAASAAKVLDDFYGGWAPAVPTGTAAAVSLVDFATAAPVDAVPAIPIDQDALENATRVLDADEDPFFQHYSPPVPAAPPPAPEVVDPRVEWRKQNNEILKKKDVEEEAARQKLKETAAQYLSKYYEVRTTTLTQRKTNNRKSEAHQKEVEVPVSGGPWEKINALLNLNSGNHQKDISRYKALLITCKAKNVAIHTGHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPI4</accession>
    <name>A0ABQ5RPI4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Biotin-protein ligase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000841</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03144">
        <property type="entry name" value="GATase1_ScBLP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019197">
        <property type="entry name" value="Biotin-prot_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09825">
        <property type="entry name" value="BPL_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="447"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin-protein ligase N-terminal" evidence="3">
        <location>
            <begin position="64"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin-protein ligase N-terminal" evidence="3">
        <location>
            <begin position="372"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="479" mass="50068" checksum="9EE0031AD92D5259" modified="2025-10-08" version="1">MALPLTPSDRRLTYTLKHPQNWRIKDCIAFLRPCPRPQRKRAAPMPGKAMPVPHVKAHDPAPLEVLVYCGEGAGYQSARNTLRALQSCLAPGVEARLLGTNELLEGSWRGRCLLFVMPGGADLPYCKHLNGHGNRLLRGYVAGGGSYLGICAGAYYACRRVAFEPGGPLEVIGDRELGFFPGTARGAAYPGFDYSSERGACAAPLRFRPLPPARQSASTLAGGSSGVSSGGSGDESYSKAGGIKGGDDVIMTADVAPPRLSPYHLVLQAGDGAGSTDGEYDRWVYCRDYSNGGPVFVLDAQPAPATKVVLSPPPSEQQQQLEGRVFEASSAPTTAASCGGAAAAASSRAGNGGGGDTEDWPPAPSWNIPSASAERVEALAIYPELGNALAAVRCRVGGGVAVLCGTHPEMPHEALSISVQLYDGQQAQHVSALGAELMKWEMQRRQLWAALLAACCVVMAAPKGMVVVGMAKEARGAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPL6</accession>
    <name>A0ABQ5RPL6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BAT2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000808</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="50"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="505"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="749"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="964"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1023"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1214"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="800"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="912"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="176"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="221"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="344"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="479"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="515"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="549"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="559"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="605"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="619"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="752"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="966"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1023"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1052"/>
            <end position="1063"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1335" mass="141788" checksum="08359774610299FB" modified="2025-10-08" version="1">MSDQSSNGDALSKAMRRYDREELFKIGQACQGKPLPEGVSLADFSLIDPATVEPERTFERRSTGDDGPSFKPAWAKGGRGGEGGLVRPSYPVPIRSKLVDMPAQGDEDGTSRPVAERNGTLTSRSSWREDRMVNYVDRDRDRETRAPRDGWEVARSPNGPPGLDRGVPPNQARSRWGPDRDKEGWRLASFKEDRWRGGDDSADRRGHGNDRETSGYGPRDAPGRDDRERWGRRPGGTDDGVHEDSSRWDDRRRRMNDLPPPPRGHYGDRDRDRDHRNNVDYHRTGGNGGDTYHGNSHEPEWMAEEGQGGDTRRGGGSGGGSRDERPPVTSRQPTGMATRLTAADVERERQAFRAMATRDKEPQKPKHGLFDVEEIDLDELRDEDDEDEDDKPPSKQSQSLAQQQRTEQQQLSTSAPRTAMGVEELERRMAAGTSKPVAPPPGFGPPPAPAPPPGFGAPAATAHAAAAQATPSAQAPSGPLGGGTGATGGNAAGKALLGMLKKGNNVDALVTPQQPNQPPLQPQPQPMPPQVMSNIPAPPVPALSALLPPGLPRPPPAAPTASASVGPPQQSGQQQGQQVEPSQQQAQQRQQPSRPLTLPLPTPSAPQQQGAAPLQQQPVTGSTTLQDNSAPEQPGGISGPFPSELSAQATAANATPPKPIPSAAAGTGGAVGVAVGTPKLTTVLPPMPSVPLVSSIPEGVRNAFADAKSETGLGGGAPAYSSSFGSIWGTPQFFPQHLNQKIPAAVPAIADNSGSSNATTAPATTTSSGAQHIAVSSMNPAMPLPIRPAVSALSQQLAPAQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQLQQQQQQQQLQQQQQQQQLQQQQQQQQQQLQVLLGIQTQAQQQQHAQASAGLGLLQKPGLNQHLQQQQQQQQQHHLQQQQLQQLQQQLQQQQLQGGINVRNLPENHPLHAFLARAAATNNQQQVPHHTQAQQQQQVMHGQPMVQSHPQQQQYEPLPGGGMPTLKGWLGPSVSALVQEQQAPLAQQATPPLQRQQQQQQQLGSGGAATGAAAQQLQQQPGGPPYGQRMPFGQPAVPPAPQNAMQQLQQLQQQLQLGAAQGLAAGTPKAQAQQGQANHGQGQGAFMAHLQQILLAAKQQQQQQQQQQQQQQQTAAMPVPGVQNLYQQIMAGSAGTGAAGVGAGAAGGNIATLLAMQQAIEAGAGAIRAPMNQQLPPHLQPPAAGTYAGPHAAHQPWPPSMALNPALAAAAAAAAARPAMGNVGGGGAAGGTGPSSLARFLNAAALQAAVGGGAGGPRMPLGTTPPGPGLQGDHLERLLQMQAVARQQQQGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPT7</accession>
    <name>A0ABQ5RPT7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF40">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="548"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="923"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="111"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="199"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="274"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="418"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="571"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="620"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="958"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="971"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="971" mass="102114" checksum="658191DA4F2AC17D" modified="2025-10-08" version="1" fragment="single">MFLNANAPIPFSGNLGSSAYPYVYYGDQTITSQRSGGTVASEAHGGRGVSGAQNAAPLQSIPVTPWIHSTPTTNAPARPGQPAAPGHTLISGVSDIAASNPQMEGLQLQKIQDLERDLQLASVQCEQYQQEGRLALAQVESLTTELAAESQRSEELRRQLLAFAAAHQALQAEHQRLLVQHTAIVREHEHMTSAQAASLRELEARHADVSRLQRLLEETQAAVAAVNAEAALAVGPQQGHPGGASGHRASIPPARRVSGVHLSVAEEERWALRLHKAERALEEERRVSEDLRQQLKMAEMESRAMQKKVTQLEGGLESPCGSAMPVANSDPVPKSPRVLSPLSLAAQPLGPTFSGGLIGPGSGPMHALAGILPLPAGAALGRAASAGSGVGHAHAGSAKHGTLRSAFASGARGAVSIGGAGGSSSGSGSSGATPQSHPHEEQVARLKQEVDGLRSDLTRLAATEEMHRVTARAAEDRARAAELVSLDVRRQCDQAARTIGRLIDENSELVTKVNAQANALADCKNTAASNEAMLRDLQMRLAEAQAELETAQKAQGQKQEQEQLSSDPSQHQHQQQQQQVKEQVQGHSLSNSKGPADVSCGARGNAVPAHGSVPSPQPHPLKETRRPDHGDNALLGPGREVLLLRGALTEADWKLVMLAEEVRRLVEEVSLSTTACAALQLKRQCENPPLGEALLQNHQAATGELMPKATVDSSAAAATSAAASGEPQVLPLDLPCILARLEGLAGRRLGPGETAAYILELAGHMEVLEQVLQAQKRRIAALRRDEAETGAELGLGFGGDANAGAGGGLGPGAAVHGDGRTTWAVQAMPLQNSQQHQQQAHVQADPGQVGSMQAVEERQSQVQTQEEHVRESSGTIQQDLAPLLPPQPSAVGPPAVHHIVSPFNSLAHCQESHIHPLSPLETAVQQSHNGLQQEEEQQLGQGNSGGMAADGLQPADGAKLDFRGAHRRDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPW3</accession>
    <name>A0ABQ5RPW3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001374</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF1">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="215" mass="23243" checksum="DE4ADB77DCE438AE" modified="2025-10-08" version="1">MTRLHGPPGSFAAGSLLFAVEIPGLDDGQFDLSGTASASRHRGARMKAGRPDAADIEELAQTWQASAASVRLLNDVRLLKRQGRASDASVLAQRLRDLGYDASRVAQNASNATPSSLRLAHDFVIVKGCGGCATALVVEPNFREHFCIGSVYATERYRQLLAQIPEELVAPYSKIQEMVKLICAEMKFSFEATGNSLPPWRSTASVLSRWAAARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPW5</accession>
    <name>A0ABQ5RPW5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="738"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="934"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1018"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1078"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1111"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="571"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="747"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="812"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="829"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="855"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="875"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="940"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1018"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1138" mass="117300" checksum="66DECE5FDB6C014D" modified="2025-10-08" version="1" fragment="single">MIRNNCYVAIILISIYLLAHLPTLALALSQLRTRRDAGVGGNDLQPKLGVWGRRLGRHDLSGSSIIYKVSNARRVAIANMAKVPVRMSATVTPGPTQATRDSQIQVVRDSLRNATFAKSVLVSVAAKLVRARVTLERGAFIRDCSDSSLEDYKARLGAAVGLRAADVQALCVKLQDVSSAQGPTPPVCTSATTLLVVDTTLRLQPAAKEYEVQAALAALPGGNAKLLGLCVPSQSYMPHASILLNAVLIMPHPMAIYADIAATTVATPSSPPAVSHGKGATAIRLDNTDSKMDTAEVVRWALAENLRQAIATAFGIPLEQVQLPASNLIIGELSAQPPLPSQSTARVLTAASSAPGTATASPSLSFRDGDMMHQQLARTQPWVMPVVVSASASAAVVLSMMLVSCLWARLRRGSDQKQRAAYSSRGAQSVIFHSLDLTTEAEPVASPELPSGSVAPITKELRKYVALTPPEASVPAEAPTTASCSTATAAVAATAVAVSDGTDDKAPIEKVPCQERVSCSAMTQPDRSEGGQIGKGGGGGGAGPVGPDDELSRGESLSEQKSDLIVAAMNSPRAAEAASSSSPPSSPPTSSTASVAGGSVLAARRPLQPQQWLPMPLPMCRTGPSGRVPQQQHQQQQALLKWAALRRLIEEKRAEAEASLASPSAPAAAAAAEASGEALPLPPVAVGAWTTSAPAIGSDRLKSIELPYVDDGVTAVPSGMTLGEGGIGQCRLSADAAGTSGTTNSGAGSGSGAGTGGSTGLLARVFSRKSRPSGSPALGGTEGRGHRVRQTFTGAIDYANTRAGREGDIRLSRSSSSAVSAGSVDAATKRRRRRQRRRPSREAVVAFDVDDVERGSGGTVVNGSSGGNINGRDDTDGDDDDTDGSYDGNALIHGSSSRDGRPSAGSFLAAAAWDVTPLSARCLQPGLSVLQDANGGDVMSSERWRPREGKRFSDVGSGPWRPSSGGDATRNTITGGVGGARIRVGSTAATLLPQVSMQKRNTGQGFCLASGQVEVVPQSSPQPQLLNPNPMPSPSSLLPSSSNQSSPHRQPLLSLNGSEQMPSVPVIPSELLLVPAASSSAQTRTHGSPSQSHNLSTLGLIGEDADVLIEEASRFRGNKPTPISSPRGGERGGGIDGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQR8</accession>
    <name>A0ABQ5RQR8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_001927</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051258">
        <property type="entry name" value="Diverse_Substrate_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920:SF23">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920">
        <property type="entry name" value="OS03G0707200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="572"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="602"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="644"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="678"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="701"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="227"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="365"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="747" mass="75086" checksum="BACCF194DC4D5B4D" modified="2025-10-08" version="1">MPSNRTSFRDETDYVHFSPGATGLHHLHSHRPSAPPDRSSSGALALDLPPFRGGVPRGSKTDNAARSQVYGILLLLVVAALLGSYSPVLKFLFSQPSPPSAALMTAAQALLAAAFLFIGALGGTTSSSSFSSFSSFSSFSSFSSSSAAAAAGKRRGKPPGAAAASVHPPTVGRAKPAPYLGHVLRVNVALGLNWAVCQLHSLMRLGEAAWRGVAAVRILGWLEGEGRRGTRREGQHALKHGLGPLWPRLGRPRSGGGGGRGAAETPPERDERKPHQQLAVEEGEHAGSHGTEIHATSAAASAAVPESGRDGVPAVVSGSSDDNSSSLNTAPDEGLLAARRRGSDGAAGGVSPTLSGVSAAAVTSLVPSESVGGPSSGSKPDDRGFRSANPLATPWASLAAVGLELGTYSFCAAVLGAWGVQRISATKVAFLSQASSLITPLLVALSGQRVARVVWGACGLGVLGAALVALDGPTSRAAAVAVAVAAANSGAVAAGGEGSEAAGVAALGGLTLVTADGGSGSGSGRAYTGGSRAMRLESNGVDGGVAVEARLPPVSGWSRSSLPPALSAESMGASYVIASCLFYALGTVRLGVHSGRFAPLDLAAASALVYAVLAMIWLLLEVLGTPNGASSDYAVAMLMLRDNVTLALLLWAGFGPGALSSYLQVLGQRIVPPAQAQMLYSSAPFWSALIAQILLRGRDGAMGPLAWLGGAVMLSASLVASLLEAVQSRKSSPLAGVGRAGPGSSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR49</accession>
    <name>A0ABQ5RR49_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000143</name>
        <name evidence="3" type="ORF">VaNZ11_001889</name>
        <name evidence="4" type="ORF">VaNZ11_003490</name>
        <name evidence="5" type="ORF">VaNZ11_003526</name>
        <name evidence="6" type="ORF">VaNZ11_008084</name>
        <name evidence="7" type="ORF">VaNZ11_013947</name>
        <name evidence="8" type="ORF">VaNZ11_015633</name>
        <name evidence="9" type="ORF">VaNZ11_016958</name>
        <name evidence="10" type="ORF">VaNZ11_017112</name>
    </gene>
    <organism evidence="3 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="3 11" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000120">
        <property type="protein sequence ID" value="GLI71774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI71676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="119" mass="13108" checksum="F2498264A215CA2B" modified="2025-10-08" version="1">MACDDPDEETPAPPTKKPRAASVRPGITGHEDQPRQVKGAGKSKRGGGQQGRHLAPKGGHVQKKGPKQNLAEYLTTFKCTRREFHFRKQHGMCLRCGSDQHRVDSCPMLGAEKNKGKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRF4</accession>
    <name>A0ABQ5RRF4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Biogenesis of lysosome-related organelles complex 1 subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="25419" checksum="AE48F7111A22BD93" modified="2025-10-08" version="1" fragment="single">IDEQNRTATPPFHSSTELRFGSSLVLGRSGTSTTANSTISSPTSVGTNFQQSSSGHGSSGGNSSSSCMRSSGGADDPQLASQVALRRRAQQQAETRQVGSISFDSAVTTMANVPSSTAAVVVELQDLLAAVQEQADKAWKYAALIEAATEVSLMRDAQLEAQAVWKSQVGAGLSGLMADQQQARNIVLNRSTLLAASLDKQLTSLGSQQTAFDEAAAQLSELITFTEAQAEHTAYDTMIIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRW8</accession>
    <name>A0ABQ5RRW8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002048</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the RNase T2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01061">
        <property type="entry name" value="RNase_T2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033697">
        <property type="entry name" value="Ribonuclease_T2_eukaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018188">
        <property type="entry name" value="RNase_T2_His_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF22">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00530">
        <property type="entry name" value="RNASE_T2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045676489" evidence="4">
        <location>
            <begin position="20"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="213" mass="24401" checksum="B8694FA7C50E3331" modified="2025-10-08" version="1" fragment="single">MDSCLRALALASLLCIANGARLLSSNSVFGYKSADGVSLRDEARGFDNFMFVRQWPGSFCSKHACPLISNRGFHFTVHGLWPNYKDGTWPQFCDKDYAFDEDQISDLLNELRSEWPTVYDSYEEFWDHEWSKHGTCALDVFPTEHSYFENILELHRLYDLAAVLGRAGIVPSTETVYRTKDVANAIYNAYGVRPLVHCYEGELSEIWLCLDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTH3</accession>
    <name>A0ABQ5RTH3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="204" mass="24487" checksum="45541493E2CAF56F" modified="2025-10-08" version="1" fragment="single">FYNPLSEPSVVRGVEVLLFSAIIAAFTTAVLSVFEAVVYRLIIMWLMSKHRHIAAKLGQRVKGFRHVYQVFGPIFLFRWLKHCSASEWLDWHRMCTALLDYVHPTSEVSYLSLKKTGRFWRYLLSNFPEMIDFLVSVQHEEREDFNRFIESMYEYFFDTREQHKHQVYYYVNNKFKAAFAQWLSSCEDYDRIFCQQVMKTALEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTY9</accession>
    <name>A0ABQ5RTY9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002648</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021138">
        <property type="entry name" value="Ribosomal_eL20_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10052">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002190">
        <property type="entry name" value="Ribosomal_L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="4">
        <location>
            <begin position="18"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="190" mass="22347" checksum="8AD2FD97ED1610E8" modified="2025-10-08" version="1" fragment="single">GAKLEPARMVMQGNFKFHQYQVVGRHLPTAADANPTVYRMKVWAEDDVKAKSKFWYFLRKLRRVKKANGQIISVNEIFERKPTLAKNYGVWVRYQSRTGVHNMYKEYRDVTLNGAVAQMYQEMASRHRVRPASIQIIKTANVEYHSCKRANTKQFHNEKIQFPITHKLSRPSKPEFRTLFKANRPNVAMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUS2</accession>
    <name>A0ABQ5RUS2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003739</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="EMP70"/>
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        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 1"/>
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        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
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    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044962051" description="Transmembrane 9 superfamily member" evidence="9">
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            <begin position="19"/>
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            <begin position="241"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="406"/>
            <end position="428"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="466"/>
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            <begin position="566"/>
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    <sequence length="605" mass="70132" checksum="97FF5589230E8912" modified="2025-10-08" version="1">MNQLLALVILALLQFGAAVRIPSATEVVVGRQLLSEKDHKYKVDDPVPLWASKVGPFTNPSETYEYYSLPYCQPREGVKHKLLGMGEVVDANRMASTPYQLQFRKNRQRELVCESLLDAEHLAKFRKAVKEDWYFQMYYDDLPVWGFIGRMEKLFKPGGVTEYKYFLFTHIDFDIKYHDDRVIEISVSTDPQEAVNISENVSGTVKARFTYSVKWTPTATKFEQRLQRYERFPLNPVHLEIHWFSIINSCVTVLLLTGFLATILMRVLKADFIKYNKDDPALDEEESGWKYIHGDVFRFPPQKNLFCAFVGTGTQLFYLALFIFVLALVGVFYPYNRGALYTALIVLYALTACIAGFIASSYYKQMEGELWVRNILLTCFVFCGPFLIMFAFLNTVAIFYRSTAALPFGTIVIMCLIWSLVTIPLTVFGGIAGKNNRAEFFAPCRTNKYPREIPQLPWYRTTVPQMIMAGFLPFSAIYVELYYIFASVWGHKVYIIWSILAIVYVILIIVTAFITIALTYFQLAVEDHQWWWRSFLCGGSTGIFVYCYCFYYYYARSDMSGFMQTSFFFGYMLMVCYGFFLMLGTVGWRASLMFIRHIYRAIKCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVU8</accession>
    <name>A0ABQ5RVU8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_003950</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UPL family. TOM1/PTR1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
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        <property type="protein sequence ID" value="GLI61556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14327">
        <property type="entry name" value="UBA_atUPL1_2_like"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
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    </dbReference>
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        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3_Ub_ligase_DUF908"/>
    </dbReference>
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        <property type="entry name" value="E3_Ub_ligase_DUF913"/>
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        <property type="entry name" value="E3_ubiq-protein_ligase"/>
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        <property type="entry name" value="HECT_dom"/>
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        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
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    <keyword evidence="4" id="KW-0808">Transferase</keyword>
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            <position position="5162"/>
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            <dbReference type="ARBA" id="ARBA00004906"/>
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            <dbReference type="ARBA" id="ARBA00012485"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="5195" mass="531722" checksum="4B689E7231B92F99" modified="2025-10-08" version="1">[encoded data, 5195 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVZ7</accession>
    <name>A0ABQ5RVZ7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="217" mass="23588" checksum="F7B2F596ABB8BC13" modified="2025-10-08" version="1">MRQEDTNAEQGPRCKSQLHSSEWWLAVPGPNQLPTSQPPVVFACSYGRTTPSWWVPHCAASPAPPARTALAQGPPESSRWGCDEAALRMLLQARVKMRTGLPLWGQLQRWLGGGEAVSDAQEDQWVMHSPAAHHQAVAASLPQQLVGPIHGHDVSVADLGGPWYVQLLHRSSNQVPVCVTRVSLSPSAGVKRHKGCTRFLRRHAASICGEQGVGRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW96</accession>
    <name>A0ABQ5RW96_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004403</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAP" evidence="2">
        <location>
            <begin position="1330"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="871"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="879"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="889"/>
            <end position="899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1445" mass="152237" checksum="C06B189CE41070E0" modified="2025-10-08" version="1">MTTCWLNMGRQGCLHVPIGGCSASCTTLQLPPRPCRKGRICLRYVSSQDVHASRMQFGDMAAGGEALDSGLRGGDGPRLPPRNDGKCYAAMKKGRISERPVLRTASALATQAPMYQASFPSATSADDVIPDRPSIQVEEKLRVARARGTVVTCDAAGEETTRQGRDRRRQKRRSRVLLSNTGATVGTPGGHPPDVSQHHPRPHRIGLTTQPARLLPAETPDGTTSGSSSWQERHVVGRSQLTTGAPGGVATAVGSAGDPCLENAAVAAEVGTMAVGGRRESGSGAGTDRCNEEQLPLPSVSVLPPQPAALMATSQGTATTVDCGGPCNGRDIRRGQSRAPVRRLLRHRGADGGPMRTPSQSSLMHPYLLTGILRTASSVPKLQKFIARYGSQFNNFHVCCCLNRLAKAPDARLPAQRETVQRLLDELGDMLVPLLPDCDARELCNVLWVWAKLGHTPKLEIWKAMRIALFGTNPAEEVEVVAPPQFALSSVANFTGAAVAPASALLSPSLSSSVPVGLEANKSLPASVVQTEERNSTRVWRWVQVADRPGPEIARAASGPEGGSAGVTALNSSVLATDCNSLTSRTPLAVNESSQQPFLPPGDVWPARTGSFGAASASASTALTDEGTAASGTAETAVGGKLEQAAPQGLANAAWALARLEFNDPWPWRRLAECSLSKLPAFIPYDISNLSYAFVLARQHRSHPVHEQLMCALAAAAESRLTEFCPQDISNMLWAYARAGMAQPALFAAAAPIARLMAADFGQAGLVQVLWAYAAMRVYDAPLLGALAHKMLPQLHFHDVNSRVMMCWALARFVAAVSGRSRAAGATAAAGCISLFSAARVRAGFVDPLVRLHLTGGQAAVAAATTAAVTAQGGDSDSSGGGSWGLGGDTSPSRWSASSVSPATLARAYVEDLTIRGGGGRGDSGTSHNSAASTTATTLPVDWAVDLLEQEDGSGYGGVGCDSFADVGSRLPHAAAAAAAASLAQSQVEAYAAASAAAQAVLSEMPPQLLPALCESLRPQIGTLDTGSLAVFMWALATLGHVDTDLFTTAADVLAARLHSGEVLTQYLTHTAWAFTVAGVYNSELYDALAAAAARLCDAGPSAATSLALSNVLWALGNAGHYDAELYGNIGRAVTAVAAASTAAAVAATPPGTDVRDSVNRLALVMLACAYAGHVDPELYNMVSEVLRRAGARALGDNSLANICWALAVVDHADLELLSDLFEDAARRDTAALGVRGCTQLFHCALWLQDLVPGGSQVVALLPRDVREVGRANFMELAKDPYVSDFQAYVFNALDSMRLSPSLETKTSDQLFSLDVAVSLGGDTGEAVRVAVEANGPQHYLRTHPTALEGSTLLRSRLLRARGWAPLHVHWREWQNLGGDVLRQRRYLRAKLRELMYQEQQLQQQALRRHGERLEEQQQAAGAGPRLELMLLDSTDCADVGEAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWA4</accession>
    <name>A0ABQ5RWA4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sucrose phosphatase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006380">
        <property type="entry name" value="SPP-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051518">
        <property type="entry name" value="Sucrose_Phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46521">
        <property type="entry name" value="SUCROSE-PHOSPHATASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46521:SF4">
        <property type="entry name" value="SUCROSE-PHOSPHATASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05116">
        <property type="entry name" value="S6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01141">
        <property type="entry name" value="C2.B.1:_Sucrose_Phosphatase_Li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="3">
        <location>
            <begin position="91"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="3">
        <location>
            <begin position="183"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose phosphatase-like" evidence="2">
        <location>
            <begin position="368"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="642" mass="71319" checksum="EDC76DE516210866" modified="2025-10-08" version="1">MNTALGKGSSQRVYRHQEKGFARLSARRCSLLPIVPIRYRPPSRCGATSPVSVTLQDTIRQFQSVPGLSAPGNVTKLTDDGLFQGQVVWSKFTWPAVLGGEEVYIWGSFNEWTKGVRLHKAGRDRDAVLILPLQPGTYEFKFVVDKVWTPAPHEPTVTNAEGHLNNYRVIAPTATFSIKAPRAENVFVIGNWDNWQYSLVLKKDPASGMFLAKAHLPPGQYAYVFAVDGNVTSDPSQPVYPNEEYGEVHRTWSFEPDLFRIFYCTGWDEAVLQYRRVVRGQPVTKEWQRLSMVNAPSRGNSLGVWKMATIVPTDPSESLEFTLNNGASGDKLKDDRPGSAPLYVCPFPGSFKLAKGMLRPFPRGAESRIMLVSDIDGTMIGDMSSPDAFTSSHRFAEYWENSASLAGSLLVYNTGRSLGQFVDLMKKCDGKLAIPDVVVTAVGTKVWHLDETCGRCAASGLKWIEDTNWTLSLDAGWNLDAVRRLARQLMAHYNDESTLCILDDGSEHRHRIALTADVKVLEYVMARLMEGFQREKLEVRIITSGNGSHRYIDCVPVAAGKEKALQYVRQQFGVPEHLCVAAGDSGNDILMLEGDHPAIVVGNAQPELLQWLVRQEQSGKVIYADACYADGILEGLARHSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWC5</accession>
    <name>A0ABQ5RWC5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004341</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050154">
        <property type="entry name" value="UbiB_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566">
        <property type="entry name" value="CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566:SF53">
        <property type="entry name" value="PROTEIN ACTIVITY OF BC1 COMPLEX KINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="206"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="552"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="662" mass="74000" checksum="582A719B23C87DFE" modified="2025-10-08" version="1">MKLYQRQAGFKMKLDAGQPRIVVRGLSRGCDRRLRCHAAVTTISPARAASTLTPSRLRPLGRVDDSPLDIDRDTCNIISKYTPEYVKNRVLRGPGDAVMAMLRIGEITARVGWFVGMLYADRLLGHEDTPERVKLRAAELREMLTALGPSFIKAGQVLANRPDIVREDYMNELCVLQDDVPPFPDSVAFSIMEEQLGRRLEDVFSSISEKPVAAASLGQVYKAVLRDTGEEVAIKVQRPGVEPTILRDLYIFRLVGGLFNTLSRRRLGCDARLIVDEFGEKLLEELDYTQEARNIQEFERNFRSDPTVKIPWVRPDLCGPKMLVMEWIDGIRCTAPDAIRSSGVRVDAFIKCGVVSGLRQLLEFGLFHGDPHPGNIFCLRDGRIAYVDFGNVAELSARNKAVLIDAVVHAVNEDYSAMAEDFIKLGFLAPGTDIKPIVPALEKIWADSKGQSLADFNFRTVTSKFNELVYQYPIRIPERYSLVIRSLLTQEGICMTLKPEFHFLEVAYPYVARRLLTDEDPALRERLFQVLFQDGKFQWKRLENLLQLAREGSGSSSSSVGSPGNSMDGSGSSSSSSSSGGLDLSDTAKDALRVLLLDEKLRTQMMLALTEDNKLHIAEVLNLLRLVQNDINPQKLVTEVVRDAPSIGRQLLLSWADRVLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWH3</accession>
    <name>A0ABQ5RWH3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="287" mass="32224" checksum="72A36B4C057A71EF" modified="2025-10-08" version="1" fragment="single">FTGGGQAGVMEGLQLINGRRYKFVLGSGGRLVISEVDPEHNVELELLDAETDGQCGQWSAELPPRLRRMHSHWLCRDRRVVVLRPISFLHHDIHFIVQCTAIQSTDPRRALGDATDASWGPHKYDCRRVPHYLQSRHWTELLQPHVLPQLPDRLVRLSGSAVLDILLAKFEDVRFIHVFASNADPGVLRLELPRFRLEFEMRSNGDVRSRDYRGYRLHHRQLLVSELSSGAVSSGAVSSGAVSSGAVHYTLPEFRQYLVLERIPGTGAVQGNRHEDVLVLIPAGTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWT5</accession>
    <name>A0ABQ5RWT5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="20227" checksum="97E7361929A98CE2" modified="2025-10-08" version="1">MTLVFVGRRLPILRYLSALAAYRSDFVSSIHSSCHSLNSKIGSSESTSDVSAKQSLVKTSSHTPASTEAATTSTPTPTPTSAGTHSPSTSQPTTVDDDEEWTEVVHSSGQLYYWNQRTGETTELGEPKPKSDRSSGSGGASKGGEQAHAGGAGRGADTHVDPRAEAHLEDRTGTYASIGVIVGAFLGWVSQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX68</accession>
    <name>A0ABQ5RX68_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004580</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF25">
        <property type="entry name" value="CYTOCHROME B5 HEME-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="5">
        <location>
            <begin position="116"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="212" mass="22963" checksum="5AA4F102F8B06688" modified="2025-10-08" version="1">MSACPFASINAAKDDVSPGSTGSSTLRHSSSDLQVQAARYELDIMTFTRTISTPDLHPGASVLTSTSSPAPNLSTATAVRQASALNVPRSYTSSVAPLSSAIMGQRLTRRQLRKLGKSWGLSEVRQHARKDDAWIVVDGKVYDITEHLLNHPGWEDSSAISTVLSILAHAGTDCSQEFREIHRPYPVAWRQLQAYYIGDLAPGEDIPSSKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXA8</accession>
    <name>A0ABQ5RXA8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF4470 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004810</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027974">
        <property type="entry name" value="DUF4470"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14737">
        <property type="entry name" value="DUF4470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Right handed beta helix" evidence="4">
        <location>
            <begin position="272"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4470" evidence="5">
        <location>
            <begin position="689"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="559"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1011"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1541" mass="160901" checksum="454FB155F294A1E6" modified="2025-10-08" version="1">MSQQASMSSARERGNQLYVAGNYAAAAAAYSEGLKNERGKTDERAALLCNRSACYLELRDLGPAEADCRACLQLQPCNVKAHYRLARALPQDHPDAVSAIASAIAFCDPSSRSDAMLQCYTSIRNAAVAAAAEAAAPTAINCISASGSGDDSALQPPLLLQLPPDPVCIADASSWTDVKAALRHGASFIVLRPGTYTTATVAPAAMPGVAAYTLLAVGTVVLKAAPGGSHAFWVDSDQSGILSFLMGPPPVVTLAGVKLVGSGSAAAACVSGDGTRLNLLACHVEDYREAGLLVAGQCTAQLARCTFTRTAVQAIEVREGGKLVADRVTIISCGQGVSVYGGARSVVLRDCTILNSKKENVMVSGMYENAATEAQGEDIPHRGADTIGRTVSLAAMEWGRQHGTLLDVMLLGCTIGRAGCFGVSADRGANVVARGCDLEFNDPVAFLIKGGTDVSILACRIVYSGSRSDCTWGGAKHGSGGRSVGRGGRGGRCHGGTSNALWRQSGIHVGVNYGGNVAVIGNAFCGPEELAVVDELKNLKADCHVDLVAQMRSRQMWSKPISEDRNSYHDKKMRPARCSGGGGRGGLTEEVLPSLESLAQRLPAWAARGFDGGGTGGLRSRKVAGAAPPPPALLQRTCYTFPQAGWAPTSHQYYVMGNTFGYDVTGGAADLSSYGTSTAAAAATAVTAATPIRILLAACGDFRNLLATTYAARGSRGGGAAARAADAAAADADAAASTSNSAASRFRRLEFTMNDGNLAMLARNVVMLHMASELNAPASAVLAVWACHGLTAPDAELLSRSIRALATDPWPSWLTASTRLDPTPAAPEGKVAEAVEAEALLRDAFASWASCTLKLSDLLNMRQQLLGGRRGAAGEIIRLDAVKLSMGALKAQVGEVAAEALRQEVQEYIYTGSLRPDHSGGSTAATKPVPEPLVAANPTLLLAPQLQYAVYFSSSIFRALPLGAERGTATARLTAALRPQLDAVARGLHDGDISVQLVPGDILAAMLAEAPAPSPAQQPKPASDIADNQEAAEVAAPASAAASPPLYYDFIDCSNVADYVSLQGLLQSAAPLLAHDAHVRLRAESLVLYSKHVQKDPALTVAGFVSAQIRPSLPAFQELLGLQLMEAETEVWPARGVRTVWAIQTDQRSSVTANDFDFSSNVNASFADGRAQGMSADCGRDTRAVRKSWLTAPRLVLELLPACKALLFPEGLGSAGTAPTAAPLALVHLLKLALTPPAVEPLVRALVRCDSTGAAALFKWELTLHAQLQAADDSGWLPQVRRLSYAARPGWNLVGCHHQPLLLAFSRVPLPPVSPLTVGGQGGSSSTCRVGFKGPVKQLMAAFAWDEDAATAYLMLPESLLKQCATWFVTLCIVRAEADCCSSGGGGGMYISPVGVSTRLASLRSELAEPVRWRSLGQPDRFDILGMGLIDRIGHDVPDAPKAPQSWNSCVSESDGTAIVDVLAPGPLPEAAEATVTASLEDSKFVVQVKPKQGGSGNITPDEFVVQLPAAGRLSYGKPSDIWLSRKLGLISARVDLITIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYJ0</accession>
    <name>A0ABQ5RYJ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004689</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06987">
        <property type="entry name" value="cupin_MAE_RS03005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052538">
        <property type="entry name" value="Flavonoid_dioxygenase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43346">
        <property type="entry name" value="LIGAND BINDING DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G14370)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43346:SF1">
        <property type="entry name" value="QUERCETIN 2,3-DIOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-1" evidence="2">
        <location>
            <begin position="406"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="534" mass="57966" checksum="DDA9FC19C26A2DBB" modified="2025-10-08" version="1">MIISTALSMNQTPISNSTRTAYRQRNRVHSSKPWQRFRCATNASLTINESKQPFGGAIVTKLAGSAKILKGEHHIIPLLGPGKGTVHSELPFGASLECVKQASLKGQHTPLCRREERSGTGTDDAVASSRIIDVLYVLAGTGTVETADGAVRELHAGDSLVAWHTTTELRPISGAAQPPAVLKFHIPASALKLPPGATAAPSPSHVDMQILVNNCLGDYDGELSQQEVANILSRVQQRAPKAISQLHNKQPKSSPQTAEMGTASSIGRDEFYNHLTPRHHQQAQATALKPFGVFRNFLPVVARHLGRMVHHVAAPAAGAFLMLRFAQAQHRTAARAPEASAVLMGPADAVAEPTEASSRRPQPQLQQQQQQGCGVVFTPPQRTNSSLQSPTGCVLLQRALEEFKAFRFPRQTNKLAFVFDPAELGLSLSFGVEVFEPGHRTPLHIHRAAHEVFFVLAGEGIAFCNGQRFPVRPGDCVVFPPRAVHGIDNISSQSKLYCLQLMTPNEAFVEHVKSGEEMGRLDDEDLCYLTSRHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ00</accession>
    <name>A0ABQ5RZ00_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="653"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="788" mass="81459" checksum="44A0A2DBD813B5BE" modified="2025-10-08" version="1">MELLNLSSVARKKSIRSMGGAGPIEHNLSNTTVEAHLSNSQTPRPTNGHADFTAPATPTSITVDSPSAALTATAGPFLSSSSSPGASTATCPSVTGQTGRCTLDSYAPSDTLTTTCSRGTSDDGFGEGIKVATFEHDSDVFSASNGEPRGIHAHSRLGRDAYGYYGAEDALARALHLISEFGLSDDGLHHPKVLDAQAALTEAMPFVWGLRKEVDGLRAKLLDYSKLMADRDKALARLRVDVADLEESRSLYKAHVAHHLSTSATTTIPTAITTTTTITTTSVNPENPYNASANASMPVPSTVPTSNNSLPIIAYVNSDATSAAFTDTNIITSFSPKEVLSLLPVIEESISMPSSMGPADQTQLMKKSDSPLSSLQEHQAKCISADEPAILKDNGQPLPDDPSQLFSETRSNHRMAKRFLDQLQLNPSVALAPGRGVQPPPLPEASFSMKTSAALRAGAAPAATENVNSGAGAELKDPNTAVTTVGDDGEAPAASAETSAVTAVVGESPFPLAGASAKSFGLKVIPSGRDGLPNNISSGSCNSLSSPGHRTSAPRSSPLAVASFGQLPILGSPNDEEVRDDEDTFVHWAAPSLVWEGDGQHAAPVTLMAAASHGLKKLWSVVVLRGDESGGGRSYNADGGESTPLLFEKNEATNASGHTTPAATARPPLSSTTTMPSDGTPPTATVTPSQRAVGHHSYVWRRSVRNGNVAAGSIITGGDGIDDSVTGSNSARSSMGIRISPSSGVMATATNNATAAVPRTCCTAASGGKSSQWIITAAEMDVMTVMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ52</accession>
    <name>A0ABQ5RZ52_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sphingomyelin synthase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MTM1058</name>
        <name evidence="12" type="ORF">VaNZ11_005716</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingomyelin synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045221">
        <property type="entry name" value="Sphingomyelin_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290:SF62">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL:CERAMIDE INOSITOLPHOSPHOTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290">
        <property type="entry name" value="SPHINGOMYELIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingomyelin synthase-like" evidence="11">
        <location>
            <begin position="206"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI62896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="458" mass="50894" checksum="713187A969C1F8C2" modified="2025-10-08" version="1">MEDKASTTPRPDGALEEGKQQGSPRGGLGQAQQGCLLFSRLFLEIWRRVRLEVVVEWPLLKQRWKVLLFGVVMQYVHGIFTQLAHRMHQPKEQPLHDVGFDLTPELGPEKHWVSEAIFGLSFATFVLWTFSPFVSQRKRFYTVVMWTRLLMVLVVCQALRIVTFSVTQLPGPSFHCRANEPTARRPWPGHWTGHFVVDVGRQVSKSCGDLIFSSHTTFMLTGILAYNEYGSVLVLKAVSWLLGVVLSVLIVASRKHYTVDVVIAWYTVPLVFYTMYRRWTTRRPMSDFIGGPVLSTFDDVDGAELELEEVFVDRGTCGGGAFFGCIDGGTAAISKLAQRPNILKISVHAPVGVSNHHVLIGSGGVYGSFLGNEVESVASSKMCDVQGRSGAQSNSGTPAGVMRGMAHPAVESNDAWVLQQQPRQQQAGQQLEDGLQERERRTLGVGQHILSSWQMPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S052</accession>
    <name>A0ABQ5S052_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fluoride ion transporter CrcB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_006125</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Fluoride channel required for the rapid expulsion of cytoplasmic fluoride.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:76160"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="FluC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259:SF1">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="369"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="397"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI63235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="52585" checksum="96BB8834086B9900" modified="2025-10-08" version="1" fragment="single">ICSDCYVHLLRVIETRMSRASILWRIHLHLAAFSQLGVLTRIVIGRLFSGSCSGNGRWTWAPCVTGSGLMVTGDTLFHDFPANVLGSFIIGLLSTGSNLAAIYPCQCTTNNVSGLQLPSLPRNSKLQSDGELHIGLRTGYCGSLTTFSSWFMQVVILMVGRSMPPGTVRGTHWVQGMWAIYLGMSGPLFALILGQHAALAAAAFWVAAEQTSCRWQRPMWATDVPAFKPIGTLTMQSTKAKPVLEAAEVAEVAADPAPEAAGAFGSVAVSTGGNPPPPALVLEVADGKLLPANAEFAPTAATAGVGLPANTLGAAASDSASPAPIRRLTRPAKLLVDVAAGSSFLTLTGVSLAYAYLDRSTTPKNHRRYWWFSILLGPAGCFLRWHLSHLNGWRDGWLQYGTFAANMLGCVLDYVCEALMFRLAGSLTNTQQVTLQGLMVGTAGCLTTVSTYVFEIQKLALASPILAYKYFLASTVVPVALGILVYGIPAWMGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0S8</accession>
    <name>A0ABQ5S0S8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005958</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926:SF5">
        <property type="entry name" value="F-BOX AND LEUCINE-RICH REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="448"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="481"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="747" mass="79665" checksum="D7A9912E8E25F603" modified="2025-10-08" version="1">MSSEEERGLALRNNWMDALSGASSEDWSALPPELLEMIGSNLTEGAHMQNAAMVCKSWNASIATGLTSMEVDMHSDPGMWRSKIVKLQSLIRHLRCVVIHVNAAITDDMLFQNIALVQQLKGVSELKLHLREGCDLPPAAAAAIGAMPHLTSLSVIGGNLKPDAAASLLSSVAASSACDVVGGLGLRSLTLLPAVPYGLGDAEMAVLSACSSLEVLEFRAYRLTSAGLATLSLGLPRLTRLAITSLDSDSGYELACLERLAQLAELDLALDNAPIGAASRRALCAVKPGRSLSLSFRWTDKAQGLLTEYGNRLANLTHLDVGACRVGSESLFEAVAQLTGLRELRMAVYSNADHCIPCHLSRLSALAALQRFQLEKRRAYFPEARRFGESSIAVPVTAEGLRSLAGRWSRLRSLRLALSRKDYSPEALSVLSNFTGLRDLGILVERYPGSSSTSSSSSSGPQQQQQEGVQEGSGGSFTHPAHASIASSSSGSSCNVGLGRLHRRTVELPPVLDLAWLPPGLQSLELNNLLLDLDEPAASLTVAAPSLQPLPLSHLTNLDIEACVVRQLLLPQVARRCAASLRSLRLAGVVGLCDAVLSECLTALPMLRSLTVSAPGNRAVSQASLAALSASHTSREVVKGIPVTDDDGSGSTTAAAAATPWRRFPQQHHQRPLLPLLRHLTWESEDLTARGPDLSTLAPLTALRHLYLSCTDKTAERALGSLRVLQEALPYCHVDMVWTTARPGDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S163</accession>
    <name>A0ABQ5S163_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005924</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="786" mass="82269" checksum="0626EE30EC084FFA" modified="2025-10-08" version="1">MPRASSLMPHASCLMPHASCLMPHASCLVPRASCLVPRASCLMPHASCLMPLSSCLVPPASCLVPHASCLMPHASCLVPHASCLMPRSSCLAPRASCLVPHASFLLPRASCLVPRASCLVPHASCLVSRASCHMPHASCLMPRASYIVPRASCLMPRASCLVPGASCLVPRASCLVPHASCLVPRASCLVPHASCLVPRASCLMPLAWCLMPRASCLVPRASCLVPRASCLVPRASCLMPHASCLVPRASCLMPLASCLMPHASCLMPHASCLMPRASCLVPPASRLVPRASCLMPRSSCLVPRASCLVPPASCLMPRASCLVRRATCLMPHASCLVPHTSCHMPHASCLVPQASCLMPRASCLMPHASCLMPHASCLVPRASCLVPRASCLMPHASCLMPRASCLFPPASCLLPRASCLMPHASCLMPHASCLMPHASCLMPRASCLVPPASRLVPRASCLMPRASCLVPRASCLVPPASCLMPRASCLVRRATCLMPHASCLVPHTSCLVPRASCLVPHAWCLVPHASCLVPRASCLMPRASCLVPHASCLMPRASCLVPRASCLMPLASCLVPRASCLLPGASCLVPRASCLVPHASCLVPHASCLVPRASCLMPRASCLVPHASCLMPRASCLVPHASCLMPRASCLVPHASFLLPRASCLVPRASCLVPPASRLVPHASFLLPRASCLVPRASCLVPPASCLVPRASCVVPHASCLVPRASCLVRRATCLIPHASCLVPRASCLMPHASCLMPHASCLVPRASCLVPRASCLVSSCLVPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1A4</accession>
    <name>A0ABQ5S1A4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006699</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF261">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="3">
        <location>
            <begin position="88"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="3">
        <location>
            <begin position="415"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="746" mass="76283" checksum="4484B05F2C9435E7" modified="2025-10-08" version="1">MRSAIPHHWRITRLQPCATTAAATTNGTDVKRLGATLVAAGNCADGLAAAAESGDAAFSSSTVFAGLSRGSAVRSSALQRRRHDAVHYAVVGAGLAGVATAWQLMRRCPRDRPVVVDLYDAAGIAAGGSGAAAGLLHPYNPRGKLLWRGEEAMAAAMELVDAAEAAMVAAAPQPGAFGPPTLAGPDIASHQPSQLLTLPSPPTAFTNAASSLGSETASYLQELAGLTQSNAAVDQRQLKRFVWTSGLVRPAANAKQAADFAKAVTVAAVVASGDAAGESGGSGSGNAAPAKLRAISTDELQALVPGIVVRVSGKGRVREVVGGAGGGDGFITREEGGEKGRSRSTSTAVGAAVAAGADGGNLGTSSNSSSSNSSSSNSSSGNVRGNNRRSRRAAKAAAVAAAVATHGADDAVVVNVAGLLIPQGMVLDVGTYLRALWRACQVEAEARGDGSLARLRYERVESLERLRWWTGTSEQSEEEERTATGTAGEEVCCVGQYDAVVVAAGAAAATIKELKKAQLPLQLCQGLTLVMQPPLPQPTPSPRGPGPAQKPDKPSLPGQPPAPGPSQSPQLPDFGYPADSPSLLGQPYMAAQGPMQLVVGATKSYGWTPEEALAACGRSEYADKAVGEVSNQSSIDAEKDAAVKLLRVAASDVWAPLVSWRIATIREGVRALPPRTSHGSLPLLGRLVAGRPWWLVAGLGSRGLVYHGWLAQLAVDAVLCDSDEVIPKELTAWRGVAAAAAVFEAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1V3</accession>
    <name>A0ABQ5S1V3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Retroviral polymerase SH3-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006773</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0229">DNA integration</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Retroviral polymerase SH3-like" evidence="10">
        <location>
            <begin position="32"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="105" mass="11744" checksum="A45F1F1C8032091F" modified="2025-10-08" version="1" fragment="single">HNRSPVAGKPKTPYELLTGVVPDVSLLRVFGCVAYAHVPKGKRDKLDSRAQKGTFLGYEANSKAYRILLGDGRIQITRDVVFLEDQFAFSNHVRPELEVFSIRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1Z5</accession>
    <name>A0ABQ5S1Z5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="19225" checksum="4FAC9A29F9EEB4D3" modified="2025-10-08" version="1">MGSACSTDKSTETMSPHATRSKSTLSKSASVGSRRTREELASQFVAEFPADDDPDDEPSYPEGSREATLLKVFNLLDSANCGYILVPVWKDYVLQAGLTMEALAKELAVIVADQKTPGDPRVTGRQFVTAMIHMVAGYDDHAFGQFVAQTLVTYRAKGASRYLTQQQVAPEGQAASQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2A2</accession>
    <name>A0ABQ5S2A2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rubisco LSMT substrate-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007094</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF140">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rubisco LSMT substrate-binding" evidence="5">
        <location>
            <begin position="204"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="284"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI63985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="449" mass="48600" checksum="E5E6076E44DC076E" modified="2025-10-08" version="1" fragment="single">RNGTGADGQRDNAEASGSESAAGGNSPVGFRIPARRLTGSCPAPRQNCCAEGGGKDGALVRQHGCGGRDQVGGRWRARDDEGVGGLDPPSYPVSPDRGVNTDQETRGGEDEAEEAPSIAGDGMWDEAAQQYCIVARRWYRRGEQVMLCYGRYTNLELLEHYGFVLQYNPHDTALLDPALLPVPSAARTSAGSPCLTPADCFLHANGQPSWQLLHFLRYCAASPAERRSAGHRMAAGAAVSQQGDRRALQWLHVACSRQLDALPTTLSDDLQLRRQLQFQQDVQNQQQEQEQQEEQQQELQRCHRPGQQQQLSQATSREQGPQHQGQIPELQDSPQSHRPTGRTIRVSEEAAAAAATGPASKQVAPAAPAPLVTRVALSDGLTCTGLALQWRIQQKEILLRALRCAEGVLGLEGVSGAARASGRGAVVGKDLSSLMQRQRQRRPDDVPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2T3</accession>
    <name>A0ABQ5S2T3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007341</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22652">
        <property type="entry name" value="NUCLEOPORIN NUP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22652:SF0">
        <property type="entry name" value="NUCLEOPORIN NUP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="204"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="368" mass="39177" checksum="91D060D6E05AF886" modified="2025-10-08" version="1" fragment="single">MAAPGGVQKLEMNIDGHGERIRWLPAISPGPADLDGRVTHHALMAWGGQNSKEDKFLVITDLEVVSRGHTVLRVDEEMVKAFPHEGKMTDLHICEQDGGLVNVLFGTTEGTIHLLRLLLPRTPGALPKDVRVLKPPGLGQLPELDAGASEDLAVWLRPHRGAVAAVDMQQDTKKILTAGSDGSLFVLEMEKVAAAVAASANDATAMDADGDDDGTAMDTMDTGEQRGGRPNTASGSGAVPPELRPYKAGTGCVGYTCARWVDRNRFVTSQSLGGLEMWDVRCPPEPVMRSKEWGAVGLGSAERGSHRAICSLQVHFARPDVCASGGSAGSVALWDLRAASKPMSLTQPDIAAGPVWEVHFDTRESLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2V7</accession>
    <name>A0ABQ5S2V7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05467">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="1">
        <location>
            <begin position="65"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="397" mass="41326" checksum="147BF1888E6EBF6B" modified="2025-10-08" version="1">MRAKLVCPAGEGMIILSRARVHQELCLPYRRGGHCAVQRGFRAAANLTLWTSARRSLPLYSSVGEASQNLTTPTVKIKIPYRVNYGEVVRVVGSGALLGEWSADQALQLTWTEGDVWTAEVPIRAGHYEFKCIVYNPANKSVSRWEDGGNRVLDITEEPGKWDVSCQWGVTKSMVQAFTPAPTLSPSPSASELESEPAVALESESLDAAAVAEEELTPTLALSEATADIAKADTWVSLSGGAPVVPAPEAHSDSSVQIGEDAAADMPSTSSSTALLLLATVAAAGAVVAVSFLAPGGLDKTVLGERAGEAVSSTLQQSTEAGAQVRAQVQSQLAALAGHASAVQGHMVNVQGSMTDGLASLLVVHNSVLYVISQATTAAADTVANTAELGARVVRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3I1</accession>
    <name>A0ABQ5S3I1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF15">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="216"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="383"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="804"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="955"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1008"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="294"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="862"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="957"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1028"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1064"/>
            <end position="1086"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1246" mass="130474" checksum="6357EC0904008A05" modified="2025-10-08" version="1">MPPVLDDGLPPHLHRTVQATLNGKRDVFKPPPRKQAPSLSIPLTPADLDLESGPLPASGASSLYGEGSRSGPLGDASSAWNSLTHKAASVRGGRAPSLEQLVDSPPSGPNILSAARLVGSAWVRQQEREWYRGQLAKRGERVNAEAERLFLTTIGPLGMSMNIWRKKEHQVEGVALRNVMREEALELHRLRLEEWNPRLTSQSATAAVQAAVATTRENSTASSAATSTAAAASVHGDPEDTRSSTAPGGRPAGRRRCMSALPHRYMETDMDRTRIRKALDKLAAKHAAPPTSGTETTAPAPDTAASPGQGMPRGRRAGPEQVTGGSGGEGGVGGNDTAAPTSPLQLLRSLRARREARWLSETPAMAARVSAFMEVESPASPRVAAGATAGHRASAAPRVPLRAAGPPRTPHEGAATLTSLTLRPASGHGTGAGMGTGTGTGTGSGAPGDGGVSFSRAASLSQLQGSSPLGSPQRQQQTLRQQEELELAQLPPDKATLAVKLVDLYEDSCRKYRLHAEPSMCSALRRSCAAVPGEPRSHSLELVALTLTNRESAAALAEVLTHCTHVTAVTLDRCAVGVEAMGVILPALWVLPLKRLTTISMEFPPPTWPLLQRALGRPTVWGAEATWWLSLRHLALTGNGLADQGLCALVEVLTVMPHLQSLALRQCGISDLSAPRLERLLVDVSSLRELDLGYNNLGSKALGALARGLPKAKELRRLSLSWNSIGNGVAQLCWTVMRLGPRHCRLEELDLAGTDLVAEDLFALAAILRTWRKTSVRQINLSHNNLVGVAGQCLLRHLNHLMPAPSQQQQQQQDPNSNNPATSAECAGDASGPVVDTPSPTGPAVASPTSGASFRSALEAAGGPGLGGNSGGGSKPLVPILKVPGGGPPAGARPDSPVKRLTINPTPQAAAPPPSTAAAPTQPEDPLSTRPSSTSLFSNNVEAAVAAAPAAFRSAGARGGGARGGGSGGGATADQEPMMVITTGCIVQPCLAPGAKLLVHPDGRPLAPTYSDDYEDGGIPARTYGGLTDRDRERLQEREREREQERERARERERERERRLGAGSTSRISSPSQTAGSHPSSDTSGTPAAAAIAAAATGVRGSVSGRLSPGPRTAKSQSQAASGPGGGEVRGAAAEVVVEPSGPHALHHALFDLSLDLPDREVAALLVEHEVEARRHGYLSAFKGVKWRGEVCKRDGVDLSPFDPVFLGWTHSLPQEGRLELLVAALPAQLCAWLANPRLHCNAEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3Y2</accession>
    <name>A0ABQ5S3Y2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042856">
        <property type="entry name" value="RSP14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15599:SF1">
        <property type="entry name" value="RADIAL SPOKE HEAD 14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15599">
        <property type="entry name" value="RTDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="474"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="521" mass="54681" checksum="90A4D2743111F0A8" modified="2025-10-08" version="1">MSKAPPKGKGNKKDRGPLLAAHAEALRTGLGTAPDGQVVKGKLTAALNIWSMVLGGEDEREEVFKAGLCPLLHVLLERGDVEERHAAMGCLSVLAQSEKHIAEVVTNRVISTAAALLMYDHARGKLPAVQLLRLCAARQEFAGKVADVALPALVEVLKSDGLPGWGQMRQAQTHAAHTLKLLLENFEGELPDIRQARRAVVLQLGAVAPLADMVASAPRLPPPSLMPAPPPVMTKKKGKESRKSSNKREAPLGVRDAAATVASACLRYLSLVPEFVDEFMRTGTLPSIIRGLQTCGEEQAAFLTGILWETTAESNVAAAAVAAGGVVALLHIISRNLAGCKIGRPKKSSAVKAGDKKDGKGSSRTSSPIKDGKDAKGGKGGKGKGGAGSSAEVVNFPDAAVCNATGALQHLTFLDDAKHQVALHGGVPILNAALKVSNPQTYENASGALWNVGLDVRNSLMLQAAGAPTFLAHPVPNPWLTRGDEMPDSSDSDGEDGPSVPERSSPERSDAVRDRVFLTQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4E1</accession>
    <name>A0ABQ5S4E1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34491">
        <property type="entry name" value="A-TYPE INCLUSION PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34491:SF74">
        <property type="entry name" value="DUF4456 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="710"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="834"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1089"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1316"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1553"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1624"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1940"/>
            <end position="2044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2216"/>
            <end position="2265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2323"/>
            <end position="2457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2469"/>
            <end position="2718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2754"/>
            <end position="2798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="845"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1117"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1129"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1144"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1573"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1941"/>
            <end position="1985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1986"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2019"/>
            <end position="2029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2234"/>
            <end position="2245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2378"/>
            <end position="2389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2482"/>
            <end position="2492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2645"/>
            <end position="2655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2798" mass="288668" checksum="5674AF35CCAC92B3" modified="2025-10-08" version="1">MADQTFQPPVAYDADTIPPQGNMEHHIVFLLDVDALAGFIKNCGSVSLADDYSGQMERNHVSNGREGDGKILPDRLAEALTRLSQVYTQLHGLPTAVSYHMISTKSKFTQEKVEMLKAKIANNPALAEVGAGMQNAGGAGGSSKGPETRTPFNVHAEMYALLKAIGEPLPEGSAYGPSSSSWLENIGHHVQQTVSRFVVAMNAAAAHRCAKVKAATFVLVTPAPLQGRPVRPLDPLGPELLAALRQVNARFMWLDPAGGADVTHKAQMEMAVAAAATSVGEAQPIAVTESDLAEVQLALALSSHWRLEGAHADCMISQPGLILPLLPVAWLPVADVFRSKAVLETIVRRIVAQGPPDPFGPLRAGFNLVAFPGYVTQFTSQKWIYQVWSETTTLTTTSYLRGIIANRYLKLPPLRVRAEPHKAAAAAKAAPAVAIKGAWQKRGSGQNPHARPPMAPPAAVPGAGLRPGASGALPGRPGPLTTSAQVNISSGANGRQTLKVPGVGLKPPGSTPVGRMPGGGGGGGGVTAGPGGVSRPQKGQTKVIKLPARPAVPDSATNGTAASNYTLGRAAGKRIAQALEQGALTGTGPASLVGKPGLDSQGGGAAAAGPGAAGTVAGGGGGSRWVWPPLSAAELAAQAARDGGMVAVDDQLDVGELLRLEEEQMHEFISGELKAAIRLSEKHLRRARQQQQQQQQHGQVAEGQGCVTGTVQTKRDDGAHPQQHQGTAWQTEAGAGASGRSDADGRRAKRQRVAPVESLQPALCDARSRLRSKLLVAPTKLSRREERLPGWQPPLELILERARERTRTVVDSLLRASERAVSVAMRGVRKQATERRARLRGGNGAAEAGEQCGEVDPDVSPGVKMSGGGGSREGDRRGTGGRHGGAAQVAADAEGDLDQDGVYAAAIDQVFVLLQEAWAGDMMGVLRERGAVRGRPRLQLCALLYIAGRFIHAVDMLVYRRRSPRALVDLLREQLDWAVPLLCHVDGEPTMATFVSERLKPAWANTWAATLLDLLAVSYAGEDREGNNEMEFVARWDSDEDWEAGMEDAGGVEAATDALEELQRIAAAAAIAQQQGSQLDISELRASGRATSVSNGKQGTGRMASASGGGGRVIRPASGSARLGVGSSAAGGGTGPAAGGGGSNTAAAAGHAGKTSGSQPQVMEVDSLRGVVVAGSQRPMAGSRMAKHVARLDPVRPAHMMPAIIARPIIQVAPDPPPERLAALREEHRRKQRCGGAAANLADLFDKEAAAPSPAAAAIAALPAGGKKDRKGAAAAVPPALPFSMHASPDRRAGRAAAAPILGGDGTANRVVGSPGGAAACSESSTEVGPTPMDDGGGRHRSRLPGLDGGAAGGDVGGRATFGLARGLFTDMESGEAPTVKFNPGGEGVFSQVATFQIVSKPREAAQQQQKDVRREVTAVLNSNLAPPPAAAAAGVAQAPHPAAAAPAAMFTLGAAEEVPAIGIECMINERASCPAYGSIPDGSDPPRLDVAADGADGAGRGVLCPPGGEADPLSELNCDGTVASGDAVYDTDGGELACDDEERECVPSGCRLQGESHSADSLPCRRQHGDGSDSDSDGDGDDDGKTSLGGSKRAPREPVPDACSPCGFSAAVARRAAAAAATSRLAGGNSRIPVRVPGPGLVPPRSRKALPAAAAAAATTAAAAAGAAPKRNSFRPAVAKPPLPRLPEADKAQRAVPLAACRNAPSLQSPPALQQTIADGGGSLSLLFPRQAAEERPVIDSDHAQQLQPQQAEVLADQRRLEDGQGPAAEDTMYIDRAAPTSVLLSPLPQQAPRAADQVGTLAADATATAAAGAIVTLLAHDSPVKAGSQDVDVTTEICSSTGKLMKPIEASLVCGLPPEVADDLERADAEQAGTPFAKGKVLGRTPRGLTPYPGKTGAKILLSLMVTPAAAHDEAAAAWRQQDHDAGSHDLQPAQTLLQPQQEQQQQEQQQQEQQQQEQRQQEQQQHELQQQEQQQQEQQQQEQQHEPKHQHQQHQEVSELTFPVDLAPGIGLGSRARNRQAGRRRAQRLEEPPGQLWAPAATEHPGLSETEVAQHPDLAAAAAPVALVQGVGGEQHHGGPTTAVTDEAAEVKRTEGCGDADANANACDDSAALAPADVAGDRVSGPIGQAPGVLWSPSQPPCAKAEGGGASAPAVEDAPSILTAAAVSQEAEALGNPTGPSDAIMDDAALAELLAGGTMYDIVKLRGRQSQIGTARTIGDKPTPPPSQTGSKGGAPSVGGRCGSVPASATKSGVSRAIGMNDRDNKVLKQQMASASGGTSIRAGRSQVSATAVAAVMPIATTPMRATTPIGARATFTVAKMSQGRQTRGRPAATAGTPRRLRIDASVSRLPPVSHQKCQPQHRLPSAQEQRRKRRADEGAAEHDGAETSVDMGIDTGLAPNSTAVMASATADDADEHPPGDSQQPLSKRRRRADVQPPHGPGLPELGDETDGTCTVVEQIAEGRVAAAGPLVAVEGTDGEMRREEEKAEGGAMADEVMDGADLQQEAEADVQHTDEASAFVASQPASADGGVGEEAVPNEEVEQLPGSPLAHSPGQTHLSLDNRLAQQHQGQQQPLTTTIDKKDNDAGGDVGCGSLDTGDGDLQAEGKAEGAGGNATRACGGREGGRTNTKSRRRQPHEDTAAAVAVETKTTCSGVVAAEEHADDPDEEQQPVAAMEGGDDDPPSSQTAQLRRSGRLRQGKSTEAALGATTRSYGRPVAQCVGGASLAAGAPVSSVSTRGAARRLALSATAEVAGDTTPCTKRPAQRAVATRRNAEEDGDVGPPGGSRPAKRMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4I0</accession>
    <name>A0ABQ5S4I0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="298" mass="31465" checksum="D411AAC66A1CC922" modified="2025-10-08" version="1" fragment="single">MVFKCILRSRGASPSLEEVSRLVPSALALRGFRADATDPEIRAEDGNDDVPCSTSMLTDTHKACAGRSRHHASGSVSSETQQAAGQRGPVRAVQARKGERRKVLPVLAGDKFTSNVATLIVRWPHSAKPPPRLPSSWQEVCDSDRFRQLFPLRPSPLTEGAVQGLRALAAAGGYSSNAGEIGGGLPAAGDVIPQQYDAHQAALLELVDRLQAVQEHLEDSMSNEGDGTIVALTFAPDEADDGGRDIDSEEEGELSGSSCHHYRPSGREGSLYIDGVGDTAGSRSSGSRKGSGAANIRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5X1</accession>
    <name>A0ABQ5S5X1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008767</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="944"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1230"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1443"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1485"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="1523"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="684"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="841"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1142"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1304"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="686"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="697"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="851"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1142"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1311"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1778" mass="183314" checksum="58AB22BE6A8F5ED8" modified="2025-10-08" version="1" fragment="single">LSASGKSGSNSGEGGEAGGSGNFALQAIAGMAAAEGTGSGGGGGGNNFADGGDGGGGGGHDPVAAAFEALLLRPLEQLWKLYEDAVQAAATGRRTSEGSGGRDVGDGDGDGVESPKVKVMVNTAAAVGAAAATAAAAPQLVILLDGVDEGDSGAVGGAHENAMLLLLQEHFPRLPPFVRLIVTAQPLPQIRALLSRKFDPWTITPDDIRPPPSACAAALAARLARRPRLVAALHQGGPDAAASVASASPAARTPGEYDICGDGGSRLRAVAERLWRASRGWMLFLRLAEDILDATATAAATAAAAMPRKPVRAPHHGSQGQSTADELQQSNILGALRAALDELEVAAAAAAAAPLYSLYVKTVVAPEVVAAALGCGADGSSGADGLPEESLWGPTAGQLTFLLETLGAAREPLYLAQLQDLGLPAISSRDGAAAAAASSASLGLGCLITVRGYKLHGVHRSLFEWLSTGGGDEIKRLLRQGPAGAADAAAGCDSSNSFGLRRGHAALAAMTLRDVAAARSSQEAAASGRGGARGPRGPDLYSLQHAVAHVLETAACMTSEGDEEGALHLLRTLFLDFYYWRCVYQEGSAASVALAALLRHEAIFARAKPPMMSATSQLEAARGSVSGRFYKDGGVTAAALAADTARWLQSYHGTLLRFPEAILQSALALPASSPIRVAALAALAPPRTPPSPPPSSRPTASSSAPGFGAEPGASQSTSPAGAGPASSVSPSPVAAVPAATLAQTVAVPAVVAAQLDGTAWPKPYYLSNPPPDWGACSLTMESRGVTRGLAWVPEPGLIIAACDDRKVHLLSPLTGGQVGLMEGHTDRVYCVALRPEIKPELPARSSPNNYGAPEPRQAATAAAAAAEGGAEPAVARTALVEAVEVVVASGSLDGRVALWDGSTRMQLQLLEPGGGEKIPSDGAEEGSPAVTSFGFSVVEDRPAGPSAKGSVDCLVFSPDGVWLAAGTSDGSITLWRMLDGHDALHVDGFTVGASSGRGGDSGKAAPSTTVTAVPVRAVKAWISPQAHKAAVAGLTFMDLSQLRGDGPGSRLRACEGTGAGDLPAQVLVSCSEDGCIHIWDPRVADSAGTAWAVSDPLRTFRADSEDQSQYEETGLQPQQPIMASADPWFICDFLPTPVDGSSIGSSRSCSGGDNDLRTASTSGPADTPRQLLAGVAEDVDVQQSRASYRSPEHYCLAAGPGLPAGCLLVGSGSGPVAVWDVTLGVLQERLRGHVDAVHCLALQDGGPNFASGSGDCTIRLWRHLPTADPRSWRAALQAALQQAIEDAAAAAATAAEELRRVGVEAGPEPPNKQEHEQEAQDDPKPPVPRRCRRPCQWQCVALLRGHTRLVTSLTFLTTAVAQRNNVRPRPGSFDLKYAGVRTGSGSGSRSGYGDIVPSSASRLASASVDFSVRIWQIDPTSAPGSRGDCDGSGRPVGHEGFSVNTVAVSPNGRLVATGADDFQVCIWDSVSGAHRRTLRCFYGGVRAVAFSRDSQRMATAAEDGDILIFDITNGDEFSNIGWCRGHTNAATGVAFSPDGFNVASSSEDGTARIWAIRGFQTALLEGHPSGATAIAWAEEGSLVATGAAQGDPGVQLWDARMGTRLNTYRYHMADVVLLAFGSCMEALPPPCAHSPSSSSSASDQAQQKLTESRRHVLLMSVDVRGLVCVWDVWGGGLLHCINHVQGADLTGSKLLWLQARAIHYADMQELLQCSPAGQRDEADKGGDVRQQRQQRVTTMLAALYPSGEAVEVMAAGKGLSCVAVVTDKQKLYIFRPMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6C6</accession>
    <name>A0ABQ5S6C6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycoside hydrolase family 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_008810</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF36">
        <property type="entry name" value="LP05116P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI65460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23748" checksum="B916CE9202265B47" modified="2025-10-08" version="1" fragment="single">MSSPRPPSGHDEDHKDSYPDVPLENDMPMTGHASTVRRLDLPAERRFLKGAAISVWQNAPDEASQWTAFARRPHSLLERLRGKNKQLAIDTSPDFWNRYYDDITCARQLGSNSLRLSLEWSRVMPNGPGLVDENAVQRYRDILDRCVDAGLEPMVTLHHFVHPQWFEALGAFEREENIQYFVDWAVMAFRLFRDHGLSLIATFNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6K3</accession>
    <name>A0ABQ5S6K3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clu domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="769"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="781"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1305" mass="141110" checksum="6653BB86C7F2C510" modified="2025-10-08" version="1" fragment="single">MSQQGQGQPSGRYSSISEGRSGGGGGGLGSGPRYKYKYKAEEVREAYLSDKESGLASTLRAALQSLFSEPRLPDNPYYYLASTLAAHVDQSQVWTTPAVDLLAAYDVEGGLEPLYGDLQLCKIPNFNHCFGLPHVLRVASREGASFLHEVLVNSLPECVFRPCHVTSSVVDGAEYTVQTLCSVQGPCALWNPQLLEFPEIEIRCDVVVRGAKLDEALRLFVDLVVADVRELNQIPVYFVDGVTIQDGPAAVPDPEKEPFRLRMSVEAVLDAGGADFEKTATRLVLGGKVLTVHAVLLVEGPWDPSDLEAHKLRYEMSKKTYVLHYHSDLEARSTLTQPMTHFGCDPYDALYGGYFLDVSSARQYAILYLGTDREPYHRDNLTKQMDFLRASIDVDLQHGDSLTAWHHLLVLVLLHDPTTVKVGPLVPESFPSLETLALRAPKVLGTEVGLLLSTLPNLLRINNSAASVLEGIVREVEALAIAAARLPAKLAAVKVAAEAVGPAPTPRTGDSRRTVRTPDVSRAMSVWLAAFGLEGRLLSQLLGLKDRILAATGSGTGLFAGISLMVQRLVSELPPEVPALAPAAAAALAAAQSRATLLRHLLHAAALTVAHGFVTRCGDVKWALYFLRIPVANSTDLAALQTVAAAGGGGGGLQEGGAAPPPATWVLDVGMVRGTQVRSGALAERVSTEGVMLQYAVDTKMDELLAKIWLVMTEPPLPVNPYPRLLQLLRTQATKLELWNEEPEEALASYLVNELTLLRPPAFIYAATSPPAAPRRRRRSGNGGGGGQQLSRQRGGGATAGGGANVHDSDGDEGDQLVGYGCYAAVTCVDVQALNALRTSLGVVLSDRHWQNGAYQGEVVPAIVGDVAISNALRLDHDGLGAHGGETGGVSSLQRVGGGSAFGAGGSGGVAVRRLSAPFEHPRLYGLEAEEHHLVGGPRRREAAQAFARDVVSDLLHLHVNSAHIVFSVGVVGGSYFDATELWATSTNPSSQPPTTNYHNSNNNMMEQQGAAVIGHVSYIGLERLQEELVDAALRVPEGRLLASVALYVAPARENTYVALTRHLLFQYRKVSPPLIPNVSVTSAMPQPRRPTTAEQQAELWPSAVYQRVFMSEFNAFKWYSWNSERGDPAQPYHYRAMVAAVQESAPKWEASGRVLQLQRALLQAAIALQDHSQLYELSRVFYSPAQELKCLQEVHQALLDLLSPRHEALRAKLDNERVRRLLMTYILRVRSALTSPKYNYFSGLAAAVPTVLAELQEEVLSTFTLTSRLEDLMQQLAVWTHSCEISVAAAVHAGVEDLDPLLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6L7</accession>
    <name>A0ABQ5S6L7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009037</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026171">
        <property type="entry name" value="FANCI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029310">
        <property type="entry name" value="FANCI_HD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029308">
        <property type="entry name" value="FANCI_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21818">
        <property type="entry name" value="BC025462 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21818:SF0">
        <property type="entry name" value="FANCONI ANEMIA GROUP I PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14679">
        <property type="entry name" value="FANCI_HD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14675">
        <property type="entry name" value="FANCI_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FANCI solenoid 1" evidence="2">
        <location>
            <begin position="67"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI helical" evidence="3">
        <location>
            <begin position="275"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="431"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="485" mass="51996" checksum="62ACE07525606A90" modified="2025-10-08" version="1" fragment="single">ASSEVAQHIRASESPQENVGVLERCFQALRKGTLSSPDEADGGEEGLQRRLQRATVALFKAGVSVISSEVDEAQLAAYPSILLDNLDLLNFQQLAALLSDICRGVESGSPAIPRLLQLLPKAATRLLEAGSPEDEAEEESATESDAAARHVSTALHRLTHAEWVSDCGNYAVQIMEALRDVPMTPQQLHSVVAKALSVCSCCTDLAALPSLLRQLMRMAGLGQRALVLRGVLSLFDRLEAQHRGPDGQNPAKVLQNVEGTALVIIADAFKYGSALAEELLKSVRCSAVAPSPFCLLLLFTLSRQHKLESQVVTLLRALVMAAFEWDATRARSAWLSSLPQLPRPTGPQLRAALLRAVRCSGVGGEVVVPGILALGTVLMTSYPAAEALALVQRNDAVLAEAEAEAAAAAEARRAQKRQQQQVPPLPHHHQQQQQQRQPRVPGARDMPYEPPHPGAPGGSAALQSALLGIELLHAAFEAHREARPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6N3</accession>
    <name>A0ABQ5S6N3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008707</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="1">
        <location>
            <begin position="158"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="325" mass="34788" checksum="DF43680B565CF5A0" modified="2025-10-08" version="1" fragment="single">PPPPPPPSPPPPPSPTIFFTNCEPCISVVLQPPANDVRPYRFDAATCATIQQNIADAMNAALNAANITVYSPYAPNTTLCSSLEIQVCGRFNGTYSGLIEQNITNTIESLLLTFMAMASGGEICRPGLEGYNVTIHTFDEACLNLTNSRSCKVPPPPFPNCTCNTTQGVLPFTVSPTYYPQNTTKFPNSITEYCFTINSLPASEIVPSTCYKGNAVLNKIEWYANDTLRSLVKGFTVIPAGGQTSRVVASSWGATGFNTLKVSLNWTPAQANGGVVCVALQKPYTMNQLCLGGQCYASIFNRDIESNAGAGYCCPLFPTAPLPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S766</accession>
    <name>A0ABQ5S766_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009375</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051709">
        <property type="entry name" value="Ub-ligase/GTPase-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622:SF60">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="84"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="138"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="191"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="245"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="316"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="4">
        <location>
            <begin position="368"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="6">
        <location>
            <begin position="928"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="513"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="549"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="1230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI65760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1262" mass="131507" checksum="C05BF6C974B9CCBF" modified="2025-10-08" version="1">MATGDSSPRVPPATPSLGQLVRSGVRLMVWGRSDMGQLGLQAFGRSEFLGGSIWTPTQLETLEDKDVVGLAGGAFHTAFVTADGELYTSGCNDNGQLGRRTPGGSSSARPTHVTALESYNVAAAGCGATHTMVLTEQGGVLGWGGNEFGQTGCGSEGPDQHHPRPIKGLGDQRVVRLAVGANHALMLSGSGGVFAVGQGSFGALGQGEGLLENRSTPVPVLPLMPLGVVQVAAGENHSAALTADGRLYTWGRGKYGQLGHGDWGNRPRPVQVASLRGIRTKQVSCGADHTLVLAEAEAETEGKGGTGGADSSACRSLVYSFGRGTWGATGLGSTDNTCRPSRVQGLESQDVVQVSAGSRHSLALSASHQVFAFGNNDFGQLGLDPGLMSNGRGLPDTFHSSGGGGEGLLLVSPRRLTSLPALPVLAVLALGDASMVLLQEARPGENMVAGPHPARRAGRHLMPYDLPDLRHLAAAAATASEDGRMVREVVAAVEDVMSSPGYVLALFAEGKSSSSASGSGSGSASQTGTTAKAGRASLHQSLGVAVTQEATASRSSQEAAAAAGDSDKAGAEGGRGSEGSVPFVPVGMDVSAIQACFAALLRLYNREVVAALGNSLMKLLDAIFLLSSTGPVPGSRPASGGIPVGLGGIAGGSGGSGARSVLGTTVATPAAGASLRSFTGAAAAGVGVGAGSGDSVAGARLSLLRVLFVVFQCPLLSGSGMGLGLSSSLASRLISVALRLPGDALQLLCTWLVSLPLELLGGRVVRPLQQYLSKLAEGGMVGPDGRAEVLAAARVMAGLHAANEKAGGKLPFSEFNNQQLSTHVNLRSEYLLWLEVAGGRSTPPSGSAAAAISQPLMSLCQTPFLLTPEAKSRILQGEAHIQKEAEMNASAREALLQGFHPIYALFLDISVRRASILADTAQQLASRPPHHLKRPLRVSFVSQGVAEEGVDQGGVSREFFQLLVAEIFQPQFGMFTYSEESRTFWFNTSACLDDGAAGEFRMVGLVLGLAIYNGVILDVHFPKAVYKKLLGEPVGLPEMEEAFPTLGRSLRAVLAMDPDQVEDALCRNFEVQYDYFGEQRTVPLIPGGTSIPVTGANRAQYVQRLVEWTLNESVETQFRAFAYGFHQVCGGPALSLFRHEELELLICGLPHLDFGALEANARYEGGYHRQHPTIVMLWQLINEMDLDQKRRFLSFTTGCDRAPVAGLGALVLVIQRAGPDTDRLPTAHTCFNALVLPEYSSRAKMKAKLLTAIENAQGFGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7Q0</accession>
    <name>A0ABQ5S7Q0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009595</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF150">
        <property type="entry name" value="MYB-LIKE DNA-BINDING DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="110"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="110"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="660"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="894"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="987"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="513"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="626"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1008"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1056" mass="110541" checksum="E5D22122694C7A4C" modified="2025-10-08" version="1">MMDDDNNSDSANIPNAGDSPGAGRSFRRERGLSRALSVLRASTKLHQQKRGSSNHMRIMEDEVGLEAEEGSTSIEESSRRRNGSGIGSLPRNLNRQRAQSVVAPVEGQQRRNAPRGDWSPAEEAVLARKHMELGNKWTTISHFLPARSDNDVKNIWHSTLRSKNSERRSFLRTYARAVRDCANDAEARKQAYEMAQRVCGPPQPIEQALAAVQQQYQQQLQMQATQQEDLLGDGDSPEFQPDQDRDVREQEAEPPSCPAGGGTRLAPDCTQLIAAFQPGSQDPLMDPLRSGTCSRTNSAQDSQQLSGVKGAYARGLVCSGAPEITAGRPSTSPLDVLDAAASGSCLPYSAHGQARGSLTLNRGSSHSLGLSSGHVLVGDEPPLHQQKHQQTQLHHQHHQQQQQQLFHTMDLGSRTGAEVVDSSCLARLSALHLADQLLHRQQMRQQQQQQQQQQVNVLPQPTMIALTNSSPVGPLRGSALQHQQQVSWLGQSGFDTLGPAGPVNRSSATGLVQQQQQQQQQQQVSQRGGSQVPEPSSSSAVMGGGTSLGALDLEDMLNLGLDLTAEDLAMLSQGGGQPHRLTNSGAPDLTPALGGISSSHPAEQLRAVQQPNGPPGHLTGSDRQAQRQQQQMQQGQQQYLQQGDAFDSLLAELEQRGRLQVQPQPPLAGMYSLQLPDPHQQQDDVLGLGPLASPYGYPFNRCNPGSLDLQLLGGVPRGGLVAPHNTCVELQSLASCSSTVEMLTSGQQAFVDSSRTPLCHQPSVVARRGSALGMFGNAPGNDSFSNASSGLMSPLVVGSGHTMMNGVHTRHGGCGAQYPLQSPAGINGGGWPPSACAGSTGIPGGPAAGWAPGVLRSSALTSGGAVRTLSSPVAFSGENAAGVIGSGDASAPGLLGSAGTAPQPGGGGGSNGSGPLARGSGLVANMLSLTVPAHVDVVTMGLDSGDLLDVMDCEPEPMPETTSGGGGVVSEEVMEMTLRPSARAALLQRPTPTSTPPPQPTLTPQMAGGRGMLQAGGGSGSSTGGAAGAQPCWSASKVSPTPLSIRSGRLGPLLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S855</accession>
    <name>A0ABQ5S855_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="516" mass="55673" checksum="B5122DA8A70A6AE0" modified="2025-10-08" version="1">MGQFYSREFDGDPYIDLMRSLPERELVWWAQKVIWLAEGFTFVDHFARTYPRLFQHKCSRCNGAGVLTCPGCAGTKVALGITSSGLDPAVSAGGQNGLPNRLQVFAALPAAAGASAGAGGTANAAGDATSGRVGECHLCGDACSWDAEDEWMDRWREWESRLAYYDKATAPLMDEWYEDIINAGNLEEDTPPVEDEPPGPEVTGRWADDDRLIAKDKKRFPALVKRFGHPYDADATLGYQIVDPTASLGENIWNMAQVYNSTPPELNPARTAHFARQGGGGGGGARGEGSGTASGSGGGPNPAAATQAALDAFDQEIIQEAAIMQNLEAAVQDLPKPHRFVATAGTIPCTECGGMAWQYSLMPNTARLFGIERPLWTDRLARLEKYWTPKMVADPSRTGQVLPYGEHGLRSLLAAAEAGEEGDPVDAVVGRAPESTARYRRDLELLVAAPELRDARIRVPAGLNNLGMYLEENGEGGTRDLAALASPLQLPETGGSWRQKQQQQKREQSELVPSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8E9</accession>
    <name>A0ABQ5S8E9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="543" mass="54252" checksum="1FF81D95622A6A01" modified="2025-10-08" version="1">MPSPAADILDVDTEPATSVEKGRRPSFKLPAPLNTSDDILDRNVWSPSRRNSSIVANSELRSSNSPRTTQQMLLSQGSGGSGLGSPKPVTPTKFEDPLLSMPSFRRSGNSPTLEPTGSGSTGGNGPIMSPAALSPSFRALSSNSFSARGSHIDFRRSVPGGSNVTSPGEAAMIGRPSSARQPSLTRGTSDFGNHLDLLGYASSVAGSESGGPGLDDDEAIDTESDTAAAGHVTGLSPRSTTVATGVGIGIGGGGSAFTPIRHHSAFAAHSLGASSRHSYTSSAARLQARPSEDDEFMVRLQQNRTSWRERPRAGSYGPVGLSFGGSLAPCEGLQNQTHAALQQLRGEFKSLSVRSMRSSAAASVTAAASAAALPLGAAAAAIGGGSGGGGHPAVAGVGSGSVGSSAGGSSGGGGDFGAFGRGGLSGGASGGGLGPPIGVYGRHSEGHAQQLSPSRLSVSYRHYYNSGDEGGAGGSGGGCPTKPELFPFYQISPPSSDLEAQAALEATGATGAAVQSEHTRSRGCARDGDGGQVETKCSGTGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8H4</accession>
    <name>A0ABQ5S8H4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cilia- and flagella-associated protein 70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_009044</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052628">
        <property type="entry name" value="CFAP70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44314">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44314:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="585"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="674"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI65496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1042" mass="111800" checksum="5EF80AEFB332A7FA" modified="2025-10-08" version="1">MAPKHKHEPPPPPPEDVNPFGIFVTITIPDATVVLDLPADAPQDPASHRKTHITLNLPGCHTPWASPPTAPSEGNVYHYTVVKHFRRTGGQDGLSQLMNGTVAVTINDSVTNAVLASATIDQMWGFAIGQNTWSADGQDLTPLEEVPAGVPKALSARLSFMSIELHERQLPPGADAAALAAAPPPESELPPLLPYSFVTPEFAETGNMVELVVTDISPVPSQLQTANDLAGGKVTVAAAVKLPGSGPTVVVHLPLAGGQAVVPALRREQMTAEACTELQYHLEDGQPLLVEVARYVAVEHFSDPAWEAYHAMAPATAAFRSLLAAGATSNAAEALPLTMFTTADGAKSYIPPYTPPPGKSKPIEEPPPPGTCFWEKIGARLSISFRLARPIIPPWQPPPPPPRSLLEMIPPRDLTPKPPPATAADDFKAKVRKVARALAEEYKSLLPPPGSLDGSGEGGGAEARHKALVFELNRSGKYAQMRDSLKASVVALVREKYRKSGSMSPNEMALLYNDLYGTLLAAMHSALNDMIDTAAARPREPLPEPVPDKQRLGQLLELAAQAEALGDVSRAEALHQRRLLARTNAQVWYEYGTYCLRRGSRGRAEQCFREALSLEPTHRPSLLALLGCSVAEGRATDPAYLEAAEAAAHRLMEVSGQPTLEQWAIMALVYKAYGDARRNELNSCEQEMSRLEQEATAAAAAAGTLGVNTPRQLDPVTGEEVPVLGEMQARAFITLATSLLKTISLPSEALLALELAAGLRHWPSASVESHREAKLISALAETALGQGAQAAEALLAPGGPMIQLMRAGGATAWRSKLLTAKLYRAQGAIEEAIRFYQEYLDEARQQGLLAQVELQVWLELAGCYMARGQQRYAADVYLLGAAAAPRCAVLWRGAGECFALEGDLPSADMCLTEANVLDPEDPRAWGWLALVALKEGRQEDAEKALSFGLRCGLDDVGVLSKIADEYKATGQLRDQQRVLQELANKLQPQSFPLRAALARCLAAQHMGPEAAREVAAARELAVSEEEMAEVAEIEEALRGMAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8H7</accession>
    <name>A0ABQ5S8H7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009202</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF18">
        <property type="entry name" value="HAPPYHOUR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="25"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI65632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="356" mass="39358" checksum="E4291E75F663090D" modified="2025-10-08" version="1" fragment="single">MNSPNAAMARRHKDEMESEAASNRFQLQNVLGKGAYGTVYSALDALTGDIVAIKVIPVTEEDREEFKQIQREITFLAECNHPNVVRYLGSYRQPNELWIVMEYCGGGSINDLLSATGEALTEDLIAYICGEALKGLSYLHGLGKVHRDIKCGNILLTTGGDVKIADFGVSAQLTATMSKRNTFIGTPHWMAPEVIQESRYDGKVDVWALGISAIEMAELRPPRWNVHPLRVIFMISRDPPPRLSHPEQWSPVFQDFVSQALLKNHKQRPSARYLLQHRFTMTQRPGVAGALLPLVQRSMRYLQEAAVAAQKRQAAAAADTVSLSGLATGRFTWRHSTVREEAPPDAAPEPDAAALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9A0</accession>
    <name>A0ABQ5S9A0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipoxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_009787</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01756">
        <property type="entry name" value="PLAT_repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052970">
        <property type="entry name" value="Inner_ear_hair_cell_LOXHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901:SF3">
        <property type="entry name" value="LIPOXYGENASE HOMOLOGY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901">
        <property type="entry name" value="PROTEIN CBG12474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="264"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="475"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="604"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="728"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="876"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="1002"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="1128"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="1250"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="1370"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="1497"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="1624"/>
            <end position="1740"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="1749"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="1872"/>
            <end position="1987"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="1998"/>
            <end position="2114"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="2126"/>
            <end position="2242"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="2269"/>
            <end position="2383"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="2385"/>
            <end position="2519"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="2582"/>
            <end position="2698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="848"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2238"/>
            <end position="2258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2714" mass="298934" checksum="568F2C743E0C1E49" modified="2025-10-08" version="1">MDFDDVDFDNRQPLGLGLGAEALEERAAVPLAPSQQPSLDGQQALVDPPSRVASTGSIGIHDGALHADPFESARLLPVAEPSGLLRQPSVIARSTLEPAEFGLLVDAAQVGSSPSRPTSASGRPPLPPGKPSPLGMQAYPSRSPSLRRQSSETGNRKMGRGELTFQMSPGQDNTRSYGEQAGTPKLSRRTAEETAKITGSPVPPGVTFSPDSTAFRRVTIREERHGPPVRVLATGDVYVQDTPMDGPLLSKDEEDNMAKSFRSGIVHAGYLHKLVGKTPLDVHWKKYWFYVSEDRLYYTGNEKNSTRVRYICLDRVPVRPLPHSRTPRKGWVGTVPDIGIALVDPVNLHQKPGFPTPIKSPRGNLALDKSSVIGLVSGSHTYFLAASSHEEAMIWLRVLRETWYHCFKHTLRASNLRSQQGLHLTSKVMAENIALRESVRELAAKAQGFDSEYWRQWLEEKARNRFLEQLNADMAIYELEVKTGKMKGASTDARVYMEMYAPHEEGVSTGEVRLLDKDTHTKPFGRGNLDLFLVPCRNVGLPNRIKIWHDNTGRRPDWFLEYIRIRKKGAINWTVFPCQRWFSTHLDDCRISRILFAGHATPYIQYKVVTYTSDIRGAGTDANVHFVMHGTLGDGARHILSSGTDDFERGMVNEFTIDDEDLGDLLEITIGHDNTGHGPSWHLDHVEVTNLKTNVTYLFPCRMWFDTRVGDGALERTLQAADSLMRMAGYVFYVYTSDIRGAGTDADVFIVLHGEHGDTPATVLPSQLEHFERGQTDTFRLELPYVGKLRALTIGHDNKGESPDWHLLMVEVAEEEEAGEDEDGRQGGRIRLGPIIRFVCNKWIGLGHPDPDSGSPEGVLQRTLRPGGEDPRLEMTDYRMEFHTGNVRGAGTDATVSFVLTGERGDSGPVKIDAPMEAFERGSIDAFTFRMRRLGRLRRLVLSHDNTGKNPAWFLLKVVVTSSHPEEPETTVFICNRWLQDASRAVHPSVELLPGGELPPVMHYKVAVTTSDIKGAGTESKVYLELIGAAGPLGPIHLDNPAAFERGATDTFILDGPDLGELLCLRVTCDGSGMRPLWHLDHIDVWRSPQMIEPELAVYFPCRAWFDKESGFVKELYPGRRTDDVVKIPYTIKVYTGDLKNAGTDANISINLQGERGESGYLPLVANYDTFERAQVDSFDLMLPDVGRPLFLLVKSDGSSRKPTWYMDFAELTSENIPLTYFIAGMWLGPETGLEIRIPAGLRDPRAGRVEYLVQVYTSDVKNAGTDAGVWIELIGEQLTSGRQALIDTSKDTFERGQVDDFKVISQTLGPLRSIRVWHDGKGKPWHLDMIVVTAPTGEKYYFQFANWLGPSNPMAEILAGLKDPRADVKSYKVVVRTSDLPGAGTDANVYCELRGTRGNSTRHPLNNRAINCFERGQADVFEVKAEDLGGLREMLIGHDNTGIGPGWHLEQVEVTDPATNQTWFFDCNQWLDTKEGDGRTERLLPASLQNPRNNRVTYKLRVKTSDRPGAGTDANVYIDLRGDLGSTGKTFLKNRNLNNFERNHVDDFQVTARNLGPLREMLVGHDDTGLGPSWHLEYIEAFDSATGVTWYFDCNAWLSSTEEDCRLERILPASLENPASKRTNYRVLVVTSDRAGAGTDANVFIDIFGLNGTNTGRVLLNKKGNNFERAQSDTFTIAGRDVGPMKKIRIGHDNSGIGAAWHLNRVEVTNLKTGEHRIFPANCWFSKKHDDFQIERDLYPGDVPDLNVDYVITVVTSDLPGAGTDANVYVQMYGTEGEAGPLRLDNPKNNFEAGDTDTFNIRAPDCGDLQKIRLYHDNKGLGAAWHCEIVIITNKARKRTWFFYCGQWLDATTGLERILTASDTDPRASLVKYTVTTHTSDIKGAGTDANVCMEMYGTKGRSGVRELTGKGNLFERGKSDTFVYKMPDLGDLTELEIWHDGTGFGAGWHLDYVEVHSSATGKVYYFPCGRWLDSKEDDGAIRRRLQVSYKDPRSFKARYRVSVTTSNIRGAGTDANVFIQLFGEEGETGRIKLDNPGKNDFERGNTDVFLFEDKNVGNLRKIRIGHDGAGLGAGWHLQRVVVENLTTGQVVVFDVNKWFDRSEDDGAIERDLYPTQGIAPGDNAVNWRLVVVTANVSGAGTDADVFVTLHGDKANFGPYTLPAPKEAFETGSTDTFSLTTPELGVIQALTIGHNNKGFGAAWCLSRVELENMNTGERYIFNFNNAWLDTKNGTSRTMAPSSYDKNMGGSSGRDTKDVPTMAKAGEGKANYRLELATSLGPTGAMMDGEVMVDIIGSDDHTGRQLLEMPAEGFQPARVEEFGLAGLPMVGSMRQLELVHAGSRPWEVRYVKVHNETTGEKAVFVPDGPLYPGVPSLLTLWTPLPCDYRVEVQTGDTFGAGTNARISINIFGEMGSTGRVELKYDGTEPGGVGVGGACGDVCATKPFRRSALDVFTLRGLQDTGDIRQVEIGHDDTGAGSAWFLSWVRVTNLTTGASAYFLCDSWLARNKGDGFTRRLLTAMSSVDPTQPNAGTLADGTATAHVTVSPREGPPLGLMPLHDPLDPAAAAGLARKLGGGGVGGSGYKITLHTSNTCMAGTGAGVFFELIGDYGSSGTVMVQATPQQFSRGASDSFIYPRLPFLGELLQLRVGTTGAGCFATWHLRQAEVVHLATGARYVFNCHNWIDKKVNWQRVLVATAAPPVAVTVGTSIGGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9F4</accession>
    <name>A0ABQ5S9F4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_010037</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000030">
        <property type="protein sequence ID" value="GLI66279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052654">
        <property type="entry name" value="CS_Sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723:SF0">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046379248" description="Sulfotransferase domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="145"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="209"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="331"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="397"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="802"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="574"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="578"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="600"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="982" mass="105401" checksum="9145148BC91C044D" modified="2025-10-08" version="1">MALPKHHNLRIGINLLINFICIICAGAHPSDFSARIVDTASLEHFQTLSDSITSQSHDLDSSMHGDGSNMATRELLSSVSGRTGRSSGAQLTRSSPGGTLFSWGLKEWRLGRTTGDASVPEPALVNLTVISIAASRHSAIATVDGVVWTMGHNDSRGGGGHGSPPLDASGQLGRGGTREPGPVEGPLRGKFVVQVVTGRYHTMAVTADGELYSWGLNDWGQLGRQAVGATSETDPSPCTAGASCRSGIPGLVSLPGAVRVVGAAAGRYVSMAVDESGRLYTWGHDGCSNGGKVPKRSQAYMPRLVQGELAGKRVVAFDAGYVFWLAATAEGEVYTCNSQDDGYAGTLSNRRPHNNAGELGREGDPLLPGRVTGALAGKKVEAVAAGREHAVVSTSTGEVYTWGSRELLMGRDGSSKEPGQAMATMAGDNIRFVSAGEYHSGAASEKALYGWGSNGYFCTGVGRGNPVQRLKAGGERGDVFQPALSQGAIARGGWQVQAMVAGFQHGLAIAANKGGSGSGSAREHEGSLFERGAARGLGSTTNNAHGHIVAANVGGVGSSTGSSLSELLPVDAEAVASGDGGGGGSDGGGGGDGGRGGVGDSDASTASAAGSASTAANLPRFSDTSAPDYHTRYLAPQLHPIELNASEHWRRHVRSPRYELIQAQSPDIWKLLPPAFDLRFKNPCWTQEGRLRCIPFFHILGVSKCGTTDLYHRLSKHPLFFKSRNKGPHWWDECIHPPKGPCTAPPNGDFDGYVDLFANAAESIRGNPQGITGEASSNTFTSAAGVYLRGPSMDRNTTVTMPELMREASPFLRNIIIFRNPVDRYFSAFYYYRWWTPEESPPGPEEFHETAQKDIATWRTCVDQRGLKYCVRHYHPQQLVKGMYSEFITDWLAHWPRDQLLFLRNEDYSAKTREHIEAVFKFLGVRQPSEAEWMKVVGMETRNRNSNKYTAMLPETRAMLEGFYQPFNERLAKILGDDRWLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9V6</accession>
    <name>A0ABQ5S9V6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_009823</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF9">
        <property type="entry name" value="OS06G0107000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="5">
        <location>
            <begin position="54"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="441"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="495"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="580"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="810"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="444"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="495"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="588"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="621"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="649"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="661"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="685"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="705"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="768"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="840"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1004" mass="100845" checksum="637512D54F8EFFC8" modified="2025-10-08" version="1">MSIPVHIIPKPTRERKAEHDRAVLAKQLLLARYLDPWGPPDIVWCHSHVLVRGYKVILNVYDLLPPRLNGCLSILRLGGAFHSGVQVGVTEYAFGGHPNDDLGITALKKPLFMLREEAEAAVQHGTDEDGSAAANLDWMPTLRSRTVVGWWMGPLTRLEGLLRELRFDESWIGSEYRLLSRNCNHFARAMCTALLRNQLFKPAPGCSHPEGMVPRSITRLPALVLALGYCAPCFVRQLNAPMPLAPHSLNIFHGHCRKARLEAPRSRLLADDRARCKGDTSGTFMVSARASASATAGATGVRPSSKTSGLSIDGSDTAAAATATARPTATATAGGGGYTINWPGTSPLPPANAAAVAAAAAAAAEGRLMRPRLEGRYQSLRVETAPRPSMELPSRGAFRRCSDDGGGGGGGGVDTLGHASTGCSTPDMAAIAAAAAAAGGGAGTSVTASNSQLNGNGRQCPHNEQLPSSRATSATAACSSSSGAAATTAAAATLLPQPTSAGPPTTSTSASTSATPTRCTSPGASVTGLASAHATVFSVSLVPPVVVMEATGSGSPPAASSAAAAPVAPVATAAAAATTATTNSSATQPRSSGGSKFLNNHSRSGRRIFPDPRVASPGPDGHPDGTHDHINAFSGDGGAPHLQTPESSMLRDHARSDLDQASVTAASAASSHDGDGGVVVQRLHTTGSAGAAAATASPRHPMLPRPTSSRSQQPMQSPRSSRNGLPPASTRLEGSPSKLATTVAPLPPPPAQLALQHPSPMASPFLRSGGATADGAGSGASATATNSQSPSEVPRPWSVLSGGFVDLLFNPSAAEPPPGVPNQVGIQGRGTSPGVAHPGASGGGGSGSGGGSRRHASFTGDGGAIFSSTANAAVGGLETVRSGDLTDDAQQNPHRAAGRARASTGMLHVGVGVEAAGSSATAGGVLVTSVAGTPSLSAASTSQRRPAVATASTVSRPAARVGVMPHGGPATAFVSTVPSTASAAVAAVADGAATPTALASRRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA06</accession>
    <name>A0ABQ5SA06_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_010187</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF63">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI66395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14375" checksum="09903B8E455564EA" modified="2025-10-08" version="1" fragment="single">RHQIMLRDRKGFVRVAVEEGVDGGIVPVYHFGNSRVLDFGPQALSSLSRSMRAALGFLHGTWYLPLPRPRPIFMVVGQPIPVPRVPWDDPKYEEVINEVHKRVCDALQKLYDNHKTEYGWADRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA14</accession>
    <name>A0ABQ5SA14_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MHD2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010102</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057984">
        <property type="entry name" value="PATROL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008528">
        <property type="entry name" value="unc-13_homologue"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31280">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31280:SF2">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25761">
        <property type="entry name" value="TPR_PATROL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD1" evidence="2">
        <location>
            <begin position="749"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="3">
        <location>
            <begin position="1071"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="989"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1235" mass="131040" checksum="B0B5F7618F1BF90F" modified="2025-10-08" version="1">MNRDVWSERRELLEYVLKEHARRGGRVVAAPQTSLYEPRVLDQLNITALVKQLQHGGILELARIMLPASPLSANGPGPGDLVPVNSRGPSGSVAGSSENPFGQTPAALSASASNGSLGGGGGENPFVQNPTGSRHAPHASGENPFSVTGGGGSGVSGGGVGGVGTSGSGGANPFHKPPARPSNGTISASDSAIFGKKKGSLNGGGAGGAAAGGASEPPVTALSTTTKLSYSTTASSSPQRNAAGLIDGSSRRRADVDGAAVGPRGEAADGAGWGPQQPPRLDLPQLETGQTDDQLRNLAYLLFAAVAGGTGNLQDNALLPLMRQQLLIDEAHAVDVSRVLKHLQPGGEPHLIDQPHPVFSGGDPGGAGGWRPSQRASLELLLRLISVVRPSDFEAVNPTKAFQGFLRWKDVTVAVLERQVATAVEAGWTGDNAQLKKMLARMHGAARRADVRGEADYDDDEYGEATRVFADVAGQLLAGCNTVLKFPWAVRVRLCEILVTALFDTLEAGIYIDEASLIVQFLDCVFFPALGLSPSASQAVNAWVHFSMYVGTGCKEQRLVKHLKQQIAKLATAAAEAPLRGSDTFGLALDGGPPPAEELLRDSALASQVANHIVEWVYGRLCSYHVAFPRGENLAALLDVFVFACKSRGDGPQRLAELLVEAVCKSATSSFQQQMRARMEPGAPNEARLLEMASIVHDIHDADANTFCPALAPHMPAALAVAAVQMHILYGQYLAPWLSIPKTISPAVLDVFRTANVLERRLVGPITAAGGGGVATSGLPPPAVADLLSSFRRWDLSSPLKAALLQWVEVQVSNMTTWTARALQTEKWKPMGSGPDAAHTSSAIEVSRMTMEALDALYGMDVPMPSEVPQALLEGIDAVMRKYVTHVNERLGALQRLIPPVPQPVRYKKDIAVKNEQAEREAGRNSVKGGKGKVTFLASVPTVESSPDFTNVSASLTAETLTAAACSLHFLYGRAETLVQVARERQLQSAAAHSLPPPQPASTAGSAKCSPAKSEGSSSIGEAPEPLLLARTALMTGMQYACRFLATRVVFWDQRTPWLELLYRHHVTLPASRIEPVLDGLHKVLAATCPGLPDAVRTAFAKHLMVASVQAAERILLDGGPCRWFMPADVQTIDQDMHKLRALFYAAGDGLDRELIDSELERVQRLLPLMRVEVGPLMDLLKTARTHGTAQLMAPSGGPGHVYDESTILRVLVHRPERNGSKMLKSSYKLPKKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA42</accession>
    <name>A0ABQ5SA42_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="125" mass="12906" checksum="08A93F288A25881D" modified="2025-10-08" version="1" fragment="single">PKKRMFGTGALEDLLPAIDEVDVHFDHELLDFMDLDEQQEQGPDATAVAAATAAAGGNSKGGGRSEGASGGVSRKSSHQAGSSARGGSGSEKEAADATPFFCLKETNGLATQVLPPQLFMSRNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA52</accession>
    <name>A0ABQ5SA52_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Smr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052772">
        <property type="entry name" value="Endo/PolyKinase_Domain-Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535:SF1">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="1">
        <location>
            <begin position="40"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="134" mass="15138" checksum="452AD5A882718A80" modified="2025-10-08" version="1" fragment="single">LKGLSKLISLLRSTMQNENRRASESIFFEMNSTLLQVWTTDLHGQHVREITYHLTKTLMDLYHGGVRHLKWTIITGKGIHSGPEGPKLPAALAEWLGEKDLTANTVEQQDGQVQVVLSAETFARLDVANWYLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAZ7</accession>
    <name>A0ABQ5SAZ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011320</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF35">
        <property type="entry name" value="PROTEIN O-GLUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047401117" description="Glycosyl transferase CAP10 domain-containing protein" evidence="3">
        <location>
            <begin position="33"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="4">
        <location>
            <begin position="307"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="432" mass="49075" checksum="334F5BAEE8BCA6BB" modified="2025-10-08" version="1">MSNTDHYTKMELLAFKWLIVLKLLFTILKVCAEQEEGSSAPDAEVQHLTDKPGWDRAWTHAALEPLYNSSLYPDVDVFHARVRFPSALGIANQLRKYHLNSTRVKRGTAVVVVRAGKVYGITFEDFSPAFRIRLDSVVSELQRHQDAGYIKLEDTVFIYNARDVPVCTLGYCLAPLFSHIKDIRNGRPYSDDMLVPLMSYPFEELVEYPQDKKIRQGVMASYMDEAVGSENTCRSLVHRFARQDTAGEYIKVLDSERLMETDKDRNGLKAAHVSGIVAPPPPPDELVRYSLVMSCDYQTANPGLASLLHTNSLVLKGRSAWHEHYYRALENGVHFVEFEPGFAEEEVKDALKPASQEKVRAMVEAAQRFAYKYLSQRSRALFFEKAISEYNKLFGPGYMKATVADLPGDRNIQMRDILALNMYPSSWRQGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBR0</accession>
    <name>A0ABQ5SBR0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polyprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011291</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="314"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="633"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="641"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="677"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1238" mass="134466" checksum="1102F0EA6AA430CD" modified="2025-10-08" version="1" fragment="single">RSSAGAGMSTSESSGGSRIAKQRSGVKCTWCGRPGHVEEKCWKKRDGVAKPVETERTDVVALTLVSRTCEFIPHVTDQTKKVIAEFESGVKCIRYLPWRMEKVAALSAAVKKEISVPDPWWLDSGSKYHITGNRLVLHNFRPLPSGLKINIEVGNGCVLPAEGYGDVVPTRAPGVTIEHVFYVPGFKHNLVSVSSLTDKGFNVLFNQSEARITRGNISIVAARCITGLYKFMSSASQVAADFASLAKFPDAPTFLASEAKSASLWHRRLGHLGLTNMKRLVQEGMVKGLEVTDKELREFEGRRCEPCILGKHARDPFPASEGKTQKALQLLHLDVCGPLPLSSCGYKYFVTMLDDYTGASLVQPIKEKSDVANFMERAITMLQVQSNGTLQCIRTDGGGEFVNRQVQSYCASNGVRHQLSAPYTPQQNGKAERLNRTLLDKVRAMLAESAVPKEYWHEALMVANFVRNRSPVAGKPKTPYELLTGVVPDVSLLRVFGCVAYAHVPKGKCDKLDSRAQKGTFLGYEANSKAYRILLGDGRIQITRDVVFLEDQFAFSNHVRPELEVFSIRDGPSVPVAGAAAGNAGQAGGAGVVDAGVPAATHAAGAPAGNAGVAGVGATGGAPVIPAAGAPAGDAGVAGMDDGEVPPLMEDDDEGEEVDYGMSETDVGGDYGDDAGGDVASSSDSETGQGSSGHTGESGNLDPTPPRPIRQRKPPGWQKDYAMLASELEMKEPKSLEEAQSRVDWPQWKAAMDEEMASLQENRTWELEEIPMGARKIGLKWVFKLKRDADGNVERYKARLVAKGFTQKEGVDFGEVFAPVGKYASFRALLAVAADRDLELHQLDIKTAFLHGELKEEVYTEQPPGYNLGGPNVVCKLRRALYGLRQAPRAWYIRLRKELESMGFKPSLADPGLFVKIENGEPVYVLVYVDDLLLACKSMAVIGQVKAQLLRAFAMRDLGETRFYLGFEIERDRTARTISVTQRRYISNILEKFGMKGANSRSTPMDANLRLTAEGDPLDTSVHPYSEVVGSLLYLAVCSRPDISFAVGSLARFMAKPTINHWNAAKGVLRYLKGTQALGIKFGGGAHVQGYCDSDLAGAEGRRSTTGFVYLLNGGAIVWSSKLQPTVATSTAEAEYMATAASIKDALWLRHLLHDLGVPVGVVPMKCDSTACIAMLESPMSTARTKHLDICHHFARGRIERGEIRMVHCSTAEQLADMFTKSLPKGKFKFCVAGMGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC04</accession>
    <name>A0ABQ5SC04_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="179" mass="20051" checksum="A86DB722EB78A225" modified="2025-10-08" version="1">MMVLAANAARIHQKEKENKAARRRQAKSSQPSKARNSSRNATNSSKAAAKGYSPFILPSRFSNDDNVRPSTKVRRTSMGIKRKVPVEDKSTDGDDNNDSYDHQGKKVKSASEDVRCTRARRQRGSSGSKKKQELLEEEVSKNEKSEDEQDDATSAREDDDNDDSEIEDEHLSCLFLSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC64</accession>
    <name>A0ABQ5SC64_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="190" mass="20661" checksum="2C868B5C659AEBE9" modified="2025-10-08" version="1">MDGAKYLTSCLSKQLKDIWGLSQSQLELCQGPCLGTVPRRLFVTCRFGATKMAFGTGGKPGAPHASFGCIPLESVIERVCIVRTPRPIVGKRGASDMKLVDPKDDDKTSQCTLNKLVGPPPEELLPEVPDEVAEAVREEGMGMQLRPCQVSVRLGAAMAAMEVKAWGWEPLRLSKAPQTQIRVIPSNKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC66</accession>
    <name>A0ABQ5SC66_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011253</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="8">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="383"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="866"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1183"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1310"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="599"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="812"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="871"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1339"/>
            <end position="1348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1348" mass="133844" checksum="2A539CE1DD322C99" modified="2025-10-08" version="1">MTAEGHTARGQQGGNQSQPAPFLRKTYDLVDDHTTNHIISWGPQGKSFVVWRPSEFAANLLPQYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEHFQQHNKELLLTIHRRKPASSNVAIATGGSSASAAPGAVVGSSSGSQAAAQLLSGAQPTALQPHPFQISASNQQLPDISATVHATVPPVAAPSAPVPIPVSNTLHSHGSPGLPAAAAGAATAAFSLSPPFPLDLGIAATLPLFTSPSPSGSAAAATVATGAGNGAFGSAPAGLQTLTLGSSVPPLVSPSPGSAFSRPVPTGQAAVARGAIKTEGSQSVGASLLGPLLPAAAPSSCGNNSCDGPAISRLSLLVDEYLKPGQQMLAAITQQTSLPNQAALGMILNDGAAAAADGGGSDSGGAGQVNGLGNIAGGEVPTPPPPPQQQQQHCSYSHSSTTAPSACSAEQSDPMVADSGDGAGGDCSGDRAATAAAVAPEGQVASGGVSYNGNKRSRCEMEPSAPDAASGVAAVVAVLLKGAPNLNPPQPQPQPQPQRQFAAQAMFETCSAGGGALGSGGGGGSSNTCLTGYDAAGNPLPAGITRPLPQHLGGTTAALLSEMAGQLGRLTALAREVEGLKEENTALKRTMEALTHAYGSVRLHGAPAGGDGAAAGGLASGPGGLAVDDATAGEAGGLVRYMYDNGMVDGGAASGASDGAAGGSSGSGPMHLEGTSVAVANAAVAPPPPPPSPVGGPMAGLMPLTQAVGVQQKRLWDQKLRHDDLEKQLQAQREASAMQAAKTAALEAALVANTAKVVHDAAVLGRVLDERVTAQSEVVRDRLEAQERANKVAQDVNQRLEARVAELESLLKALGQDACTLTDVNAGIGRVSLGAASGQKVPPLPQPPPPRPTHSLPLNNYNQKQNHINHLNCPPPPAWTDAAALDARALSYHGRDAQQQLQTTEPHGIGGAAPPPMEGHDDAVASAAAVVVAPPPPQPPQRLTLALSPEAELYAKSIEDRLWGTVERIVSSASNPADGARLVLQVAKARHDAYAATAATAAAMALSSSSGGAVATAAGTGISSGGGGGGAVAAAAAAAAASDANGHTIGGSGASDSNTPGASGCNAGDGGAPDGAIAGDSGGGGSIRVAAVTAATVSGSPLVLCGRAAPAGHDVCIADIEMFRTGQRAIDLGGGSSGGGGTNTGRYSGGAVAFSERHSRRRETAAAAAATVGEGANSREGSGSCPPPPLPIPLPPTMTTAVGLGGGGVDGSSDYISRSFRLLAAKPCFDNLAAVNQHQHLGCSQLKFHPHAASASVSAAAAGTTIVPGTALDTAVAAANTPVEAGDGEGHSTTPNDEKEKGQRNQQDQENVDDMDLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCR7</accession>
    <name>A0ABQ5SCR7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011601</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="2">
        <location>
            <begin position="101"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="314" mass="35178" checksum="0B0E5F4A57F3CC79" modified="2025-10-08" version="1" fragment="single">MQAAAQEQSEEPGEESQDGDEMQVDVGDQNVGAAGGGDESPPGRRYPVRQNRGQLPARYIAQAAVLRNIIVPESYEEAMTSKEAVHWQQAMDEEIVSLTANDTWDLVKPPLGLNVIPTKWVYAVKRNADGEIERYKARFVAKGFRQKEGVDFDEVFAPTCNQVTVRSFLAMATEKGYDIQHLDVRTAFLNGELEEEVYCDQAPGYEAGGAGMKCKLKRALYGLRQASRAWHKKLHGTLEQLGFQVSMSDPALFVSYGQSYTIWLLVHVDDLLIMGSNKVEVQTMKEKLMREYDMRDLGEVSLFLGMEVERNKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCS4</accession>
    <name>A0ABQ5SCS4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011456</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF80">
        <property type="entry name" value="GRIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="438"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="515"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="560"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="688"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="793"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="998"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="299"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="516"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="577"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="688"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="715"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="793"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="998"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1028"/>
            <end position="1037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1054" mass="112087" checksum="08B8D61442A0B66B" modified="2025-10-08" version="1">MAESHSAAREAVQRVSLQKDKDFLELELQEKERKVDLLSKSNEHLVAQVQQLKAMIKDAHVRYEGDLQRTVASLQDQYNSALELVQLGAGLATTSQPKAEPLAPSQQPSAVSFGGTSNASSTGCCTGVHRPSCCRATQQPLQQQQQKHRPACKFPHAAGCRCYPRATPPVWSPKQMQPNRNSADALDSRYSALGLNMDDLAPAATYGSTVGLATEPLAVLRDVLNLDGIHDREREQQVMRDRLPSAEGSSVAAGVTGPLLAQAQFESRLRNAYRSMLLQLQHRYQLERERQEDAHKLAMHEQEARHEAELREMAAIQDAELGKAARQMERQTAACREEMQRLEAELVAAKSAVDTNRSQAAAARATAEALQAQLQLQRTTHEAELQHVRQGSMPLGEHDEIVRKLKTEWEQALQQRESDYNALLSELAGLRRQLSSVAETTGSSGNSCGHGPSIVSPAAGTFDGQPQPSAAASAAAASREPQTAASVLGRDATGRRVALEDHDPHQLTEDLRQTRETQQLHQEQQQHLNRHQWKQHRPEGAVSGVPDVLLVTGNGLRVRLNPGSVHGEEYSSSRPTSPAGSAGSAELPPSPSASSSRLSALVDAVVQGSSAARGGRDMQPLLLATTAVKADDIDADLDVCAAGGGGISSGGEDLRSFGGTFQGHVWRQQQEQQQQQQQQQQQQQQQQQQQQQEQQQRPQQGYDRGGSAGGSSKAGDGGCDRGSGGGSGGGSGSTSSPDHHASEVTLGRWQPEAPPAGPSADAARWAAAASHQCLRQGDSLDVAAVAVTGAAARRASKPSPRAAAASSRREAHATVVVTRRRSPSPSPTSSPRWCSGRPSGSTWGKSGNPAAAVVLPRCIRCGLNDVTSPGCCRFHPALLGQPGGLRFTPEWMTCQAAGHTAHAPGCFVRSEHFYEPLGLQPQPPLAGQPAVAQYQFAEPKPTVARKLQPRDNPAWDKVVAAGREDDDGVIRGVPALRRDRVSTGGGNDGVAAAATQLAGGHKAAAAAAATDQSAPRRLSMARSPVGGAARGASGGGSDSPSPKPRTRLPSPMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD46</accession>
    <name>A0ABQ5SD46_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012089</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03467">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496:SF23">
        <property type="entry name" value="3-PHENYLPROPIONATE_CINNAMIC ACID DIOXYGENASE FERREDOXIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496">
        <property type="entry name" value="FERREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="30"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI67801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="149" mass="15787" checksum="6D3FCE110C847E69" modified="2025-10-08" version="1">MSNALCTRSSGVARSVVSRRAIRVQAAAWTKVGSIAGLKSNGGKQVVEVGGQKVLLVELDSTVRAVSNKCSHLGLPLVGKTAMFQADIKDKCVVCPAHGTYFDLETGAVKGEWCPKLPNLPLVGKGPAQKPLPTFQVRVSNADEIEVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD52</accession>
    <name>A0ABQ5SD52_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="203" mass="20938" checksum="003D2B5C0A734249" modified="2025-10-08" version="1" fragment="single">DDWSDPYELSDRASALEHSLRRRSICSAQFSLDADESHSSCTATSSHVKLGSALRALHVSVSSEHTVEGPLGAPRACAGGVSRRLEQLPWGADFLPPYMSAFGETSDEVSRVCGLPPVPSLITLSGDNESFISTAPGTAGNPLVLGDSFSPARAPAMGVGLVAGGPGADATHIGAVAQCSPLRGVVKVRKTEVSNEEKIGEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDR7</accession>
    <name>A0ABQ5SDR7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF35">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="356" mass="37553" checksum="F4A598E8BC884FAE" modified="2025-10-08" version="1">MAPISPEDAAFGAMLGAFVGDATGAVLEFNEKHICPETVEWALTMPGGGVWGVGPGQITDDSELAVALAAGLSQHDPSAGFPAEEVARKYGAWLESHPFDIGNTCRTAFSAVSHHTDAAGPLAQVMRSQAQFSRDSKSNGSLMRITPLAVWGCRLSDKLLAEAAMEDAKLSHPNVVTLHANAIYCIAIKHLITRPADADAAIQAACAWAQDHACDEVRGWLKEALGDGPGPAANRMIGFVRYGFFYAFRHLKLRTPYQQAIREVLLLKGDTDTNAAIVGGVLGALHGASGIPSHMAQAVLGRLGQLDAKGPKRPEWLQPGRLPEIFAELYGRATGMQVDVRALPGLPVAAAPGAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE35</accession>
    <name>A0ABQ5SE35_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011839</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43948">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43948:SF10">
        <property type="entry name" value="MRJ, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="50"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="260" mass="28943" checksum="C6CCBA4503D6635D" modified="2025-10-08" version="1">MSKASFERLAAQLRRSEDDVSVKLNVKDMEDSKPNLTAAQRILKAWETKDYFSLLGLPEPGADELGRPVWSCTEVEVSKAYRKLSIVVHPDKNPGDEESRRAFEVLNQAHRMLKDPTSREDVLRGAAEKARKRREQQEAAVDLNTRMQLNAAKNERARELRKAEGQGLQAHVLEQMRKRQEEAKRRAEAAAKAAQARSLREAGSAYDENAVNPSHANPRGAGQGPGQQAGKSRPTPESDSEEEAGAAAARNKRRKKPSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEP7</accession>
    <name>A0ABQ5SEP7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011906</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="306" mass="32104" checksum="8EFA65F53C3445AD" modified="2025-10-08" version="1">MMPVAEALGTLSPGRRALMALGAQLGMRIGLLVTPFAEMGNLRDITSDPQRSPFRVRPGWGPVQACKAFLATACEVAEALGCLHSAGLVHRALKPSNVLLFAAADDSRGFEARVTDVGAASRAAAAGWAQWDPSAMATDPWVAAFLAPETVYRPWASVAAQMFPASRSSDVFAMGALMLTMLIGHPPSHSGSGHMATSPPAPTVAGDGDVAFQVSECACAAGPGGGDDISCSDGRRGGEGSKAPPHLQLHGSLQLLISRCMATEPTSRTSIDQVVSELRRLQQEQEAEAAERGKMEEDREACRQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SES8</accession>
    <name>A0ABQ5SES8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="208" mass="21394" checksum="DF39AF5C33124873" modified="2025-10-08" version="1" fragment="single">YIAAASPGAAVTAQTTTTSGASLQSIEALGAPAVARPTVIRAAGAAGGGYEDIASASWMRPAAQLPICPAWRPVGASQPHRWVVTRYPQWMGLATVEAVKAVSRVGSHNTPWILTPQATQPGMAPPQTQISFRMWIAQAPTSSPPASRAICGVGISGGVPAVVKFTANVSSNDNVGGSNKKFRWPIILSSDDRAAGMYGRGEGVIFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEZ4</accession>
    <name>A0ABQ5SEZ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-related protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10221">
        <property type="entry name" value="ASKHA_NBD_Arp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="49775" checksum="1A67163F3859FE27" modified="2025-10-08" version="1">MQPIWNINRPAVVMDCGTGFSKIGFAGNAEPSFVIPTALGPGATSTTAGAAATAGFGSRPLLGDLDFTIGDEALAAAQLAAASAGSGAVGAPGAGGGGAAAGALTFPVRQGVVMDWDAMERFWQKCLFHYLRVDPEEHSVVVTEPPLNPPESREAMAEIMFESFNVAGLYVGMQAVLALYAGWATADRADKEAAKAGAGSTSAAGSNGGGSSAAAVTGHMSTATAHLTGTVVDVGEGVAHIIPVVDGFVLEGAIKSLPLAGRAVTGVVQQMLRERGEPVPPEISMEVCRRIKEGHCYVAPDMLREFGRYDRDPAKYHRALKLPNPRTGADFRVDLGYERFLAPEVFFSPGICSSAPHGTPSLPRAVDDVIQACPIDTRRALYGNVVLSGGSTMFKNFGRRLAADLTAATSRRLQPGATAIDITVRSHAMQRFAVWFGGSLVACDPGFRAVCHTREQYHEYGPSICRSNYVFQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFB0</accession>
    <name>A0ABQ5SFB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012881</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="1773"/>
            <end position="2115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="81"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="672"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1059"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1178"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1371"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1441"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1585"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1853"/>
            <end position="1877"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="389"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="2101"/>
            <end position="2128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="681"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1063"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1186"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1206"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1223"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1234"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1255"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1452"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1476"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1523"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1585"/>
            <end position="1594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1853"/>
            <end position="1871"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="2130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2130" mass="218513" checksum="76D67CA6B7F95DEE" modified="2025-10-08" version="1" fragment="single">MMAARRESKGRRQKARQQARFFKEIDTTLEALGIGTAPDPGVSAAGGSLSGLPPSPVSSTLSLTLALSSDAQLAALATPTALSSRAGTPPPGPGAGGSVTAASAAAGGPGDFNRRVTSDLSLRSLSDLNSGCSTPERLPSTAGMASVGLTPPPLGSWVTCTRTTHHDGSRSSFLGITTENTTAAGVASPMRPPPLPPRGPQVSAGAVPAAPAAAAAAGGGTHPLTMAFPGQPHPAPLDRRRTVPQVAIAAATQSTAVPTAQSHSHRRSVSYEGETAAAAVAATTEGGNREAAAAAPAGAVPYHPTPVRGGWAAVVMSLPPAAVPSTATAAAALGGVGGCAAGAVITGSTATEGLPPPTSATSRSYGALTDRDAFRRQLGSVLVGHGCSVEELQSQVEEVEERAESLEEEIGALADAAARMEAQLHTLLDLQDAHLQLLSPGLHFPTRGDLADPFSWHELSEQIAALDEMYRKVKEVLRRAKPLSRSHLAEDPDLLPRLHALALRLLGGLAEAAEALGRAACWGVRGLGLGVCGGAGRQAGEDEVRLGGKTVRRLLDAVDHISKKISDDLGGKTNVEAREMLKKCQEISLELARAGIQAAEVAAPGLLAGGSLGRHSLAHEKSNQAKRLIALWDEAARQTDRIAKRYNRQRLSLYGQLCDAYEKAGLQALDMTRGSVQGPPGAEDGNTADDATTTEGATSGDEGGDRLDLASSFESDVRGDAGGGGSGVGNGGTAVAWGGGAAGAWGGGGPGLGRQCRGASAAEVEAHAWMPSLFQVLLSRASEAHRNLRNELASVDGVANSLGGGASDEAAAAGLSAAASAGALDPHNSGSGSWATAGMSYELSDELMDELWGLLDEMQQTFAELCLANLRRTVWLCAGGSGPLPLWALESVRDSAEAALRFIHTHEHSSMALGDMLAVETELGDETQTWLHCTWNDYTTLTRRVVDGLLSVRRQINVRRTVTSGMGPEPANRGAMFIGAARGNNAVASGYGRGQPVLTAAAALAMPPGRQQPAGQAGRGQGVGFWARGGAPGAPVLIGAEAQSGRQQAPPSLPLPYMNTPSQQQQVGQQQQQLPSTVAGSDPWVRGTTTAAAVTGESGVSKPPAVPAAAAIHGSGVGGSSAWKDALLAQHAAAAAASAADGLVRTGRASIDAAGPTRSAQPAVAVATATATATAAAAADMHGRLHTSTSSSRLSATPGPNSGTQRRVAAAAAARSAAAASRAGGGRHGGGGRGRGRLNGRGRSRQREPDDADEPMPPPPKLPPPPPPLYDTQGELPPGLDPWPRLRPHLATDAGSACGAPDDVSDVSSTTTAAAVAATSATLPLAISVPLSSRPTSPLLATWASPPSPSQLQMAGGLSWASVARGSGGGVTPLTAAPGQRPSTAGSTGGGGGTVAIHTTPLKSPVEIGAAAQQASAVAAVEAQATAAAATTGFISSPFKALAQQMQSRSPSQPPRLSSPPSPEFRPGGVPGGSGARAAAGAATDPNGGTGPRGRTPSAGGSNLPHGSGELYHLLRNQQHPSPTTPTAPLLPPHSPVQPGRHSVSVSVNVDVGSAQESGPCSFQFATGAAGPAASGRCGTAAAATSTGNSGCSSPVDRGGGGGGHRTPRLGSPHGSGSVTLAATAAANYHGGANGTGPQINGHTANGVAHHYNQHCHHNGTAFPPPYQPQQAASPAITIETSSTAATTAPHVLHNTPSLSTALSLGHDGQGLARYGSYSLQGFDSFGGRGGGGAVQEESSLLADPEVAELFRSIARDPFCGIRWQDLSHGLRRGLSDIVGSGSIGQVFAGMYLHGEVAIKVIDINRETKYDLRALRAFKHEVDLNKRLNQHPNIVRFIGVCAEYIQHAARQSGLLGDGPDPGDSSGAASGPTGHRLNPVSGPPMLAIVMEYCPMGTLYNMIGAAREAQKARQAGLTRGVGPRWGRGTDFLQHWTRRLEVLKGAAAGLEYMHRCDVIHHDFTSYNLLLEEKNGRWNTKVCDFNLSRVIARGRPGAALAVPNSGGAWSPRWQSPEYLAGHDYSCPTDVFSFAVVIWEVVTLQRPWDAELQDARCESMAMIRMYAEGKRLEFPKNVQPDLPVLQQIKELCERCWLEEPQERPSMRQVADELMRLHKVVQEYERRIVEETRAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFM6</accession>
    <name>A0ABQ5SFM6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="3">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_012878</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF13">
        <property type="entry name" value="NADH DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="12">
        <location>
            <begin position="55"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="13">
        <location>
            <begin position="487"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="346"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="357"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI68456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="556" mass="59826" checksum="54E6D54FDC3C8B13" modified="2025-10-08" version="1">MKALLASLPAITRQHRQFLPTIAASIRIVQREYASIPKAAPAAEDQLPLRTGRPRLVILGTGWAAARLAHDIDPKLYDITVVSPRNHMVFTPLLASTTVGTLEPRSVAVHMNDIQPALSSPSNSLFIAEAQAVDLASRTVTCRSADGVSFSVSYDKLAICTGSQGSTFGIPGVLEHAHFLRDVKQAESIRQRLIENLALAGIPGRALPDWQRLLHVVIVGGGPTGVEVAGELTDFISNELRALYPERARGMRVTLVEARELLGSFDASLREYAARKLIRRGVVLKKGVVHEVTERDVVLKDGTVLPYGLCIWSTGVGPTPFTLSLPFAKTAVGRIAVDKFMRVLASPSHHQRRDQEQLLQPPKPQQQKLSASGATANATSAVSAPSSSTGASPSPDSPAPAPALASMPGMLADESDTPSTSRLEPVPHVYALGDCCANPDNPLPALAQVAEQQGRYLARILNAAAKGPVYGETTVVQQLAPEFRYRHLGSMATVGGHSAVLELGDAQRKQLSLAGFLSWIAWRSAYLTRLGSIQKRLAVAFDWTVTMLFGRDLSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFN7</accession>
    <name>A0ABQ5SFN7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">U2A'/phosphoprotein 32 family A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_012322</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003603">
        <property type="entry name" value="U2A'_phosphoprotein32A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00446">
        <property type="entry name" value="LRRcap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="U2A'/phosphoprotein 32 family A C-terminal" evidence="10">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="276"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI68001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="316" mass="34126" checksum="1ECFE2FA38A8DC3E" modified="2025-10-08" version="1">MEARPAAGVGPRLPGRITAELILRSPQYMSCIKQYEIDLRGNKIAAIENLGATQNQFDSIDLSDNAIVRLDGFPKLLRLKQLLMNNNRVARIARGLEEFLPNLDTLILTNNRINNLQDIDPLATLPKLEHLSLHGNPVMTKPNYRLYVISKLPRLKVLDFKKVKQKERDSAKAIFACAEAQLTHASKTFEPEEDLAAVQQQAGIAGPRENGDQQMQLDGEGQGEGEAAAARATAAPSQEQLVAIKAAIANAQTLEEIHRLEEALKTGLLPSEIRVEGRDGGKGTAAAVEPAGESATGGEVEANGNGGSEQDVMDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG49</accession>
    <name>A0ABQ5SG49_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="136" mass="13821" checksum="107ECD88995ED166" modified="2025-10-08" version="1">MQSFLWGPAGPSPVTAFSATTVRQRAPRFAPAPTSSGSDPNAKPAFSRNLPPSASPPTFSYISTWVRSSPNVPAATAPSPRFRSRTAPTTASRLPPLPFAAPPKLIAFSLGPPASASPNSIAAAADAAAPMSTPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG95</accession>
    <name>A0ABQ5SG95_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_012894</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050974">
        <property type="entry name" value="Plant_ZF_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12506">
        <property type="entry name" value="PROTEIN PHOSPHATASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12506:SF79">
        <property type="entry name" value="ZINC FINGER C-X8-C-X5-C-X3-H TYPE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="173"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="178"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="621" mass="62042" checksum="A84D9E1CEB3A54D3" modified="2025-10-08" version="1" fragment="single">LPPPPSDAALRRAVEALFLVPDGGRGGGGEYAAAAKRTAALSLVKALHTQQGGFLPLSRLTDIVESSMTGAPDLEQLLSSEPELFEIQEAWYGGPRSVRLRASAVMARAAALGNAAGAPTATTAANGSSHSHAPPGASSEPLPRRPNALNCPYYMRHGTCGFGATCKYNHPPLTPRQPAESSPSPSTAPVVSTATAAAPGAGPQRAPAVNSFRQQQQPPQPPPPPQPPSNSHNSVSVAVQNSSTASTVTPGEGSAAGSGGGGISVAAAGSRLSPTTLEVKVVTVACTPAHVDMLQHCFAAPQVAVACKIRHAAYTHLGSAAGVEVGQVCLVQLYAPAVMAAAGNTGGGAGATHGSGLDASGVSASIPGGPAAGGGGGNGAAGSTLRWPATVYLVDTAAGEDKTGMLLPSLSPLLESDVPKVVYCGSEVVPYLPRISPLHDISVLHAVLVRAPHAVLGPAAGAPEAAAAMVPPLATLLQQHRVMTTVGAAAAAVAPAHSPLWAVRPLADELRQQAVLEVVHLASLWTSLMTRLAALPQQLSQAAADRIYVEHSERSAAVASVSAAAAAAAAAGPPLMHSAARAPPMAVPAGVAVLVPAVPVAAAGPGAPQQRAVMSMHRVGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGD7</accession>
    <name>A0ABQ5SGD7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="410"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="612" mass="63340" checksum="21B6F87D17670306" modified="2025-10-08" version="1" fragment="single">FYASVGGTGGLVNRVCKVMPLGARESEALGCDTLNFAPLWMASPAMNLFGGFQDHLGSQNDDLLGQTPEGQQALIFAGADFALHGLSQEHKELLRLCSQHVEQEREAQVRIQAFEAAAASSTNGTPSHSGLLGQSQSLSPPPLTSQQAPSSQSSNFTHSSVSVPHTPLKPFDSSLAGLGSSALSARTISTGSLSLAGAVMSASAVDASTSSALHNNSPMTSRPMLSPSDHMLMVPSASILSVHAPSPGQSTPGQVPPPPPPSTPNSMLGAGNSGPTGAPGASGSGGAASGAGQGHLTMAFAQALAQAAAERGVDPATLVQLLQSPKLVDVLLSKAGDLQLQQQQQHQLQSRMSAPVPGEWAPHHSVPASLAGPAGLQHFRPPPPPPPPRPSQANAVAAAAAANATYGVSASSGGCGTPSSGGASAASSGGGGNSSGSSLENVPSMQQVLSSHIVQEFVSARGVLVQLMMSAYAFLNSDSMREFYEKLMPVSALYDEMHELPVVQKRNRCTEMSFLRVQLMQNPSFMQILQAVCALRHHLHSLSRKVLLMNQGPDGIPIDLLLGSMSTELFHLMKGVLPQEVCDFFGNCLPHPRLAVLVFAEFTSVDPMPTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH38</accession>
    <name>A0ABQ5SH38_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pseudouridine synthase RsuA/RluA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013259</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF87">
        <property type="entry name" value="RNA PSEUDOURIDYLATE SYNTHASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047208775" description="Pseudouridine synthase RsuA/RluA-like domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="4">
        <location>
            <begin position="337"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="4">
        <location>
            <begin position="479"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="207"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="448"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="469"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="651" mass="70011" checksum="E252DAA6726EC373" modified="2025-10-08" version="1">MRWWAPQLWLQIFVASRGTPSCSSSGWLGTLPSHPDNWRALSGITKESRPRRSMYAKQAESGKAGGQQTTFGAAATPAAHYQEKALRAVTLLERFLDDFETAPPVSFIRSVGVPTAMSWMRRRGPDVPHAVLYRLFRQRQIRLFDGEKVRRVSASRPLENGDLLLFPNWLVSSKQLRKQQQQQLVRKWDPAARATAAVIAAEAVRRSDPYDGSGDDAGGPAGDDAVAAGGGGSGGGGGGGSGGGDGTQVYERDSVRQLSHAAAGPGPGSGRDVAAEKGEATGWTAAAAGTARPVTEFTAQAALPPQLRNLHGQPQQQGLVLTPERVRRWVLAVHPGLLLLNKPAGVRVHGRAAGDETNRSWGSRGRSAGGGMRPPVPLTLDDVMHHGLRYGEADEPRLVHRLDQAASGVMVVARNADAATWLCAAFRDKADQALAAGRKGEEDIEVRAEEGDGNDAGLGGGLQGGARTSQREAHRRTEERPSIHRTYWAFLAGELQPRQSGRISLPVVVDGVACPAVTAYRVKATGCGVSWVELTPETGRRHQLRVHCARKLGAPIIGDERYGYRGLPPRLMLRDRLPPEWWALLGDDPRVVRRSHEAAGEVDPLPPPVLLHSRELVIKRPGKAAVAAVAPLPRYIRQLIEAAGWPMPSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH87</accession>
    <name>A0ABQ5SH87_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_013895</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF699">
        <property type="entry name" value="INTEGRIN-LINKED KINASE-ASSOCIATED SERINE_THREONINE PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="11">
        <location>
            <begin position="78"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="368" mass="39050" checksum="92856BB6E095DF79" modified="2025-10-08" version="1">MEGEILEDAIAKDQSEQGTLPAGHRKRGPEDESVEAARDAKEPRLQEPAVTSGGELPSSAGQPNASPNAPTRTGTFLTAAYSEDKGCRNTMEDVCVLSLDAQPDGQSETCRLAYFGIFDGHGGVSCASFAAQHLHARVMESGLIQKGLLAATDRKPDAKACKTAIVEGYKKTDEALLKECAAKNWQDGACAVTVWVLQELVLVANVGDAKCVLGRLPDKPTYGAVDSKPKAIVLTKEHTALLERQRIEKAGGTVVNGRLAGRIQIARSFGDAAFKKLGCSNTPDVTAFPLTARDAFLLLGCDGFWGVFGAQEAVDTAHSLLAEGLGDKAITNRLLNVAVREKRCKDNCSVMIVRFGNSPGAAVQPDTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHC5</accession>
    <name>A0ABQ5SHC5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Clp1 P-loop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013403</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Clp1 family. NOL9/GRC3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF3">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Clp1 P-loop" evidence="7">
        <location>
            <begin position="202"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="122"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="392"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="641" mass="66967" checksum="55E4A874BC995A60" modified="2025-10-08" version="1">MGHEKKRRKSGGPSPAQPLKERRIDGEYPSWKDYSGYTVLQLLESQSVLFVGEAILQVLHGAVRVAGKLVTVESPPLVLSTGGRFSLAIKIVGEAYVTQVPFAASQEVVEVSLQSRPPLPLPVRSVGTAADRQGPRTGQGDQEDAAAAVPSTAPSPCTFDLYHPTDQRAPPSMLVSKEWAQAIEDVCSAACLGVPVVVAVLGAKGMGKSSLARLVANRLLDECPTVAFLDTDVGQPEFTPPGLLSLHLMQPGSPVVGPPHAHSRQPWASRFVGDVSSEHDPQLYLIAVQALYASYWNWAQGLVAGGAAWPPLVVNTHGWVKGLGFDLLTQLLRVTAPTHIIQIRGGVEKKNLPHGAFWCDPQNARAAAAAPLMIITLEALVSPGPQTPSGRGPKGSGSGGAGSSSAVTLRSLKAVESRALAWHAWAKRVVGAEPAWGSYESEDFWHNAGELAALAPWCISLDDVHVQLLGGSLAPQQIGRLLNGAVVGLLATSPPPRQASRTRQPAAAAAGGAASTSMAWWDLDAATASAAAAAYPRGRISYIGASSTPPPLLPCVGLGIVRAVDMASRVAYVLTDADADVLECVGVFVVGRLELPGSLVVGGGVAWPYQQLFGLVAEATGAKSGKARKNLSRLSLVELAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK54</accession>
    <name>A0ABQ5SK54_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="882"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1140"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1046"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1151"/>
            <end position="1161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1193" mass="125188" checksum="71F012F3F5AC181F" modified="2025-10-08" version="1">MEPPSVDPSALTFTEKKTLFSSISSADHATPPNSRPAHNTSLSLSSFRHQLEHQYPKYLTAPAANKVHLAPAKLFGEATAPGSRAAAFHRLDRQQSHKLILEVATQTESFSPAATVAAQHSNPPDSPDAVALLWLPRTSVQAPAEGNSDQANPVVMKDAVILSGDGTSGRDQSLGSRTPPGLIADLLSQFVADCKEKGVCIDPRVSEALRAAQLFGKDLVTLAAAHSAKSGDIDIDNAATQQKLMTPPKRALSVEQSSCLRSSQDASGAARCTSPSLPYREARIGTTSATSSGVDGGGGVDVAAVTLVRAANHYQVDSFESSRMGSGVTALQEQVRQLRLQLQLSALSRYDANRTLGSALRRSLTQVQEVIASAATATAAPMATSESGTRSASTAPAAAAATVAAAPSDVGLAQQLWMEQLRRTELSGMCSRLQRQCQGLLSYISLLSGNGCVSMMGDVATQPTQPQQQQPERALQCTERVRMRLVLLPARGAWTAWAAGRGGKERGLDRLEGQVDCEESDNDEPDAENVATCELPVRVKVQLVVRRTRALVGLAPWSASEHPSLCRRAAAGLALSPCHILKPPNPQATQHPRNESVCAGVGAGAGAGAGAAATRVIVGMVKSQERISPEGMVVSAADAGPSSGLRNRLTQHHVPGWSGGQHRGHEAQADSGLSAAVRQLVAAVEEQTVTASAAVAAAGSSSSRLAVSPVSQSILDGLRGVLLQLRCNGSEGDAGPCPSAEGGVCAAQPTSPHRNSHAELQLPQLVRQEVLPPGDAAVGPGAALSKRRMTTSAAALAAAAAAAASPPRRTEAAEVVSGDSLREDGVSSVWAWRTKSAPASGFTFADTGGDQIPYDNDSDFYKIASEARLPLEDSHVCGGGGGGGGVNRRMHERVTTPPCSPRTSHCTPFASMDEDDLRAEGSGLGLCSAATATATGGDGSGSGSGCFFDAEYGSRGDWYGDEEFYLDLEDVDELAVAAAVNGPTALLSAVRVLREQLLQAHLEIVKLSTQLLAVSHQREGLRAQASQRDAALQLVAINEGDALLEAERLRAQLASERAARARLSAQYEDLRAMLSLGIHDGAFGAHPQHPCDYHHHSHNHSHHSGSLQRLSHMHLYGHNYNQYHQGCDLQQLQQLLLQHQEHQNQRSTLPPRHRSAPRHRPTGAFSARAACDNGAVVGSQAGPALASVLLSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKM3</accession>
    <name>A0ABQ5SKM3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014798</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="79"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1035" mass="117453" checksum="C75C7DCED0116C4D" modified="2025-10-08" version="1">MLMAQLLLGFFCGITFGIAVASYVALYAPVLVDVYVTEYLVWILMGSSVFVLITVLGVTIGRYCSTVSFTQSNHDERALQLFVVLPGSRTLTFSGSTDSTVGQLMDFVSSNSLFTFQPGSVWRLTSSRGDVLSDRDKHLGLYGLHNQYHVQVLGRLLGGSGRLPNKRPASSKRDNISEARITVGQFGYAWLKEKMAGRVRDVVADRQLRFLRDICLPPGNHVPPSLYVLRKMLGIPDPRDFEQHICLSEKCRFPPLPTKDWMDHRDDTCNCPEKHKRFKMVTTSAGAHPLPNKRYWDFGVENIIQAMFSDPLFRQLRGTGRDNEPDDFYGSQMAKDIDAKTNGQLMQHANSAYDLGFDFAQVYQFKTHSIGLLLMRCTDLPVAHRSKRRFTYILTVIPGPEEPSSMDVYVSRTLDAFKEYGPYSESSLMVMGNDRQLFEHKIFLSAIFADTPANRKLSLWNSHTSATGCGHCLLKGVSGEGGGMYFPGYAVDADVNCIFDGVIPDMENAPLSARCGDPRIMVSHEMHLKRAALAMKDPQWTGIVGCKGLSPIIRELDYCNYNDVFVVPVAHAALFGVIKRFWNTILGKLGRHVMDYTILGPQRKIMAQRASHIITTNDLNRPYRDIVKHRGTWTMEDWLHWLDSYSVYILHEEDSLQSSTPLMPVVQRVVDGITKSFNIKVMWNNLRTALLHYFRYDPSDFSADANNHAARLLKSFSIAAEHVFGIQFCTYNLHILVCRLREQENARGHVAFATEFWVERGMQMMKSIVKYRSPTVPELVLAGGYLASLWLTHLKAAHPETFRTFDEWVPGYRADNVQLNPSKRDVFLDEEDSEGSQLLGQGYIADAEQRSHILGGILRTEETEDFMARTNLLPSVLQDSDIYIFKRALKRRDEVVLSVDYARATKRQSYHFKCKYGPGGMVSESDPRVSIFIGEILFFALIRPRCDVAPVKVAVCNLHRAEVLRPKELYRVKDYSRRDATVNEVRQEILLNYFPKEVGMIDSKVIRCRVNNELSYFLTYVTFSQLPTNDGGEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL69</accession>
    <name>A0ABQ5SL69_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Plastid/chloroplast ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00402">
        <property type="entry name" value="Ribosomal_bL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_bL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018257">
        <property type="entry name" value="Ribosomal_bL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="Ribosomal_bL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01024">
        <property type="entry name" value="rplS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680:SF9">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00061">
        <property type="entry name" value="RIBOSOMALL19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01015">
        <property type="entry name" value="RIBOSOMAL_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="16740" checksum="5FFBC43CC6A54379" modified="2025-10-08" version="1">MLASRPVVRPFTAGRRSVAVHAKMSLHTLTQELSRSQLKKNLPSVTVGDSVKIGLAVQEGSGKTRTQTLDGIIIAEHGSDSSKTLTFRRVFQGVGVELVMPVHSPAVQKIEFVRRGRVRRAKLFYLRERIGKSAKLKEVVGVRSEDVAAPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM43</accession>
    <name>A0ABQ5SM43_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015759</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052145">
        <property type="entry name" value="Mediator/Homeobox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330">
        <property type="entry name" value="HOMEOBOX PROTEIN BARH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330:SF19">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="1066"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="876"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="990"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1293"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="565"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="881"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="900"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="995"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1298"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1327"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1347" mass="141560" checksum="F5E283E7D700E244" modified="2025-10-08" version="1" fragment="single">MGPRRGSGGEDGGRSEGLAGAVMEVASPAAAIPGSAIRLNSKTSQRGERRSDAVDNNRMCADQVLRSRAAGRDYSSGLQTDGRRGDGADDGSIREEESSEGNPNPRDQQRSGIPSGVNDLLRADILPPLASFVLSAGASGLSGMQAIRRAFPAESASVMDSTVPRTVTLYCQLGDKQELLEPHRLELRLDLGSSSLRLGPAGGVMRALGLRGADKLRVSIVRSGRAVVAAAEPTAGGWGWTVELGTRDLQLAAIPIPLVVAEALVGERTAWQATQVFPVLLDPRAEKPPGSGPRNGTFRGMAGNADRKPEWRVVGLADWLHQRGAAPGDLLQVFALRMPVEVVVALEWAPCGGLDGNKGQETPQHQQKYWHLRTTDASGGLITIVYFRWRRADDVQEGREECNQLSAGWDDLPASASPAAAVPGPPPGRSSLAAVEAVAPHAPHRGEGPAEVSREPSSRSHLTSITDNDRDGVMTEAMESGGPDVAEAVVRPPGTEASRGISRISKNPSTAMLVEAAAGRQQQLLLQEAEETEEEPLQRRGQAGEQKPSQHGLRRGSKPKPWPPGARQGQAQPQQLEAQQQAPSQPQSWQLHMWPPQRQQQQQGCTLPLQAGAVAAREVTPVMGIPGMPFGAISSLALPLQLPLAMMALMQAAQQRQLQSHVATAAAAAAGAAAAGAPSAAQTAGGALVRGTPCESRSGGGSQADLRVQGIAVMPVTVALGTIAAAVANGLALAPAARVAGANAGGGTAAQAQTRGVHHGFAVVGNGPQVPPSRGASQLPQQEVLLMGKGPQSPMDMLPGSVSTGAATSAAGFPSGSCPGGLEAVMRMAASEHNPGAAGQTATTSGVVKPQVGMAEPGYSNGFTMLPLLTDAVKNDTRPHQQQQQQQQQQHRQQQQQQQEQKEHVDERADEHGLAPHLPSPSALRYLHGCVPTKLGLPPLQPGEVRLYGVTFSACLNVGQLLAKWQAAQQHTELSGWRQITIDLPHDGANAGASDGHGGGDRGGMSAGPSGHVLPQPQPQPQPLWVAAFRGPGALLPRPVCFDLTRKLGLTTNCMTPLPEGPVEPQGLEVRRLPERPTEAGLFASRSFPRNCVICVMGCYVKAQETGLDPFVEKGYEALEGPVKQQLQLRMAAGCGRVSEAMVWRFLAMSFRMAFPGHEGGRAGCPGLPALELQMLSYGGLAACVNDPWVRGPDATQPHTRKEANCVVVPVMVRGVPLPVLVALHDIKPGEELLREYGNEWWRRQSECLGTLEDHGASPGAVLHDPNIIDALPMGDSAAGADRALQRRVDATAAATTSGLSPPSLPSGCAQAGADGQNRDPNSGVDCGRDRQQREADDRGEEKQEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM50</accession>
    <name>A0ABQ5SM50_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tubulin--tyrosine ligase-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015525</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamyl-[protein] + L-glutamate + ATP = gamma-L-glutamyl-L-glutamyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60144"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143622"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:60145"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF145">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="143"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00041448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="349" mass="38150" checksum="6FD928186A741A51" modified="2025-10-08" version="1" fragment="single">VGANGGGTSAGMWILKTGQDAGKGLSLLPADQALQYAREEQQKQQQVGGGDGGRRFQLQVAQLYIADPFLILNRKFHLRLWVLVTSDCPLRAYLHRRGLVLFSVEPYDKDAAAASATSTALTAITKAPSLPRGRVAIDKSLTHPDAAASPPSHDQTAHVTPATATVSATATAPAPAAPYQPPASHITNYARNNNTMVWGMSRMAEHLGPERFRSVWAALRAACARALAAASPSLAEAHAWLRPAIKEYGFQVLGVDFLLDERLRPWLLEFNSSPSIMVQHEDAETRKLVYEQKYGMLRDTWEIVRARVYPRGPGKWEGGSGPRGFRGRSTAQWEAAHAGEYEPLMPYMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM72</accession>
    <name>A0ABQ5SM72_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_015509</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF15">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="69"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="380"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="607" mass="67530" checksum="8330B9CDD2B065DC" modified="2025-10-08" version="1">MPSAATKKALADKKKAAGGKMSKSASTASMPTAASSASLASQGAAAADEVYSTRTVTGVLVSRPTARDIKIDGFSMGLNGCELIQDCSIELTIGRRYGLIGQNGSGKSNFLKCIARREVPIPDHLDIYHLDQEAEPSDRTALQAVIDHIQAELTRLHALEEEIMTTSGPEDERLEAVYDRIDELDPATFESRAAELLHGLGFSPAFQQRKTKDLSGGWRMRVALARALFAAPTLLLLDEPTNHLDLEACVWLEEYLKNYNKCLVVISHSQDFLNGVCTHIIWLTQQKLTYYTGNYDTFVKTVAENEVVQMKKYQKEQEDIKHIKEFIASCGTYANLVKQAKSKQKILDKMEEAGLTKPVQREKTFQFSFPECAKLPPPVLPFINVSFAYSGKPEEYLYKDLEFGIDCDSRVALVGPNGAGKSTLLKLMTGDLTPSKGTVTRHPHLSIGRYHQHSVDQLDADKTVLEFFKSTYPNGPTFKREEDEWRAYLGRFGVSGRLQTTKIGMLSEGQKSRLVFAMMCMKNNNLLLLDEPTNHLDIEAIDSLADAIKRYDGGMVLVSHDFRLIDQVAKEIWVCEKKTVTVWKGDIRDYKALLAKKMGLSGVPGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMC8</accession>
    <name>A0ABQ5SMC8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046263035" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="32"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="27353" checksum="B95C544C57DDA5B0" modified="2025-10-08" version="1">MSDSCSLRITFLALLVCSCAVLLLAADPALAELPLQTTKSPPPALLIKLPPYIKDLPLSRLPPPPPTPRPSARPPPPPRPFPRLPPSPRPRPPTRPSPPPPLPPPPAPRCSFCVNISIVSWPSFMQNTDCTYLVSTLTNGITNDLFPSSLNSNFGKFTSTCSAQAQQASVCTSVSTSALGLVSQSAVKSTVASWLQAVQMNFMKYCVSMTTSSQITSANGCFSSDEVFDMLICDRFPTPRYKLRGTSAPPEIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMI0</accession>
    <name>A0ABQ5SMI0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016185</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000102">
        <property type="protein sequence ID" value="GLI71125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13778">
        <property type="entry name" value="Aar2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13777">
        <property type="entry name" value="Aar2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.550">
        <property type="entry name" value="Aar2, C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007946">
        <property type="entry name" value="AAR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033648">
        <property type="entry name" value="AAR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038514">
        <property type="entry name" value="AAR2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033647">
        <property type="entry name" value="Aar2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038516">
        <property type="entry name" value="AAR2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689">
        <property type="entry name" value="A1 CISTRON SPLICING FACTOR AAR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689:SF4">
        <property type="entry name" value="PROTEIN AAR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05282">
        <property type="entry name" value="AAR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20981">
        <property type="entry name" value="AAR2_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAR2 N-terminal" evidence="4">
        <location>
            <begin position="23"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="AAR2 C-terminal" evidence="3">
        <location>
            <begin position="305"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="537" mass="57290" checksum="CF285C759FAEA872" modified="2025-10-08" version="1">MAGNTPYSVHLAADVAKQLVDHGGVLLVLGLPEGAYFGIDHMAFRCGPRFRGIKMIPPGPHFACYSVPGAEHRGGSMGPVAGFFFTVTTGSSLSAGVAGGLNSTDNVFVRRYDAGEELLVEPPEDEAHRYGLGVRRYEFDWGLAPYNLNAWQQWRNLTSCLDARVIDVIQPVGANICTAAEVVLDDQGLEPGGRGEGVSSRSRTEAEVRLQQQLREGRDIPRATATQIRTEASEAGAAKAGRLVEPRSSTAVSGDLGEDMAVDTTTGGAEARGGPEERRPSQRAGDDGAAGGSHRLSLGHRFCFYSEIPRLVKEAGLNPMQLTALNMDRSRQLEALAAARWGGDVLAVVGEMQFAFIAFVFGQSLQGFDQWRALVTLLLNCEHAALGVHAATFATFLAALRAQLSIALSDAAPLAAPRRSSNCGAQGDDAVASGFATQGASLVEEVLLGGSGRDCFLRHHLAAFIEVIREAGPGVVDPRIQHEMTQLRAVLDRALGWQFDGIQVRGPRMWKLTSVMMNARAKPPGYCFPSPISTLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN66</accession>
    <name>A0ABQ5SN66_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016572</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045395258" evidence="2">
        <location>
            <begin position="26"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="197" mass="21593" checksum="F0A4251C8851E8A7" modified="2025-10-08" version="1" fragment="single">WKKRLGKGLLLHCCYLAADLGSSLPQTGKTPGRSLLASSVPGRPVPRGGASSHYSDVIRHYCTKPGHFIDCLKRKADRQVNGGTGGGSGGGRHSYSYAPHHMTFAIKDNGSHLLYWHLDSESTWHVTYNESKLVDVQPLSPDKQLEIVCVGSQILIPMAVGNLSMHSNIIKNLQLSIYVYVAREPVVKIISIYQLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNA6</accession>
    <name>A0ABQ5SNA6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Pyruvate phosphate dikinase AMP/ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_016678</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056301">
        <property type="entry name" value="GWD-like_N_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054481">
        <property type="entry name" value="GWD1_pHisD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46999">
        <property type="entry name" value="ALPHA-GLUCAN WATER DIKINASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46999:SF2">
        <property type="entry name" value="CARBOHYDRATE-BINDING MODULE FAMILY 45 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22973">
        <property type="entry name" value="GWD1_pHisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23166">
        <property type="entry name" value="Ig_N_CWD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-glucan water dikinase-like N-terminal Ig-like" evidence="14">
        <location>
            <begin position="141"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-glucan water dikinase phosphohistidine-like" evidence="13">
        <location>
            <begin position="677"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="12">
        <location>
            <begin position="982"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI71457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1162" mass="124420" checksum="BC83672F9D403CB4" modified="2025-10-08" version="1" fragment="single">MSQLMIVNSSRVGFLVSARKQQPPWRTSGTWPPQATGRYWSSIPGQSHTILVSGLNCNGGGGSAPLGGISGPMQRLSSLAALQAVEAPLGAAARRGAEVGGGRTSGGRRPEGVKAKRNAKGPSVLSEIRFDDVGLHGRAEQGEDGFKLTLQLDTPRGLVLHWALDDWTPPPESTWPAGTVRVDDKACQTSFPADGRLEIKFSSDDCPSRVVFVLKQTEGGEVWFNNNGGNYAVQLRAPPLQSFVQKVLTSEGASSHWSLSQRLMLAAEVLEAADSAGPEGMALIFTWLRLSSTRVLDWYRNANFQPKDIASIQKHTGERIAEKARSSAHPLNRLLARGALAGLPRGGGNGDDIRMGILHIMRGNGIREGHRPGHDEPFLEQWHQKLHTNTSPDDVTICEAYLAFLHSANHDDYWRVLWDNGRLTREKLESWTKPIKAWPQHLPHLIPAFQGYLWTLKTTHAGADLDTAMEMAKGFMDDDLRWVLYDILAHRNDWWVPGKIVEARQRLAYYWKAPGSSRDLLLLDIALENWFRTSLERTDFDRLGRDDLIELVGLVLRSAMITYDDEDLNQCAALWERLRSHGSGSPPLPGERWAGQWSLAALAAAERLELSVAAHMDKLYGMVQPHAEAFGRACRLDQAHVANFGEEVVRGQPLFLASLLLQKLEPQLRAAAGGAPWQIVSQSASVSGGAAGQVVAVAALSEVQGHSYNRPTVLLAGALTGVEDIPPGVVAVLTRSTTDVLSHLAIRARSQRVLLATCFDDDAWKVWQDLAAAGAASAAVVDPAVGAVMPAEAPAAVAAGGDAGAAPLTGPRVTLQSPERTSSWAIAESSFRPGLVGAKALNLATLRQRLAAAGAGDTVGVPASVALPYGTFERVLAEEARNADAAAEVDRLVTAAAAAAAAGSFPREELDQLRKVVSEDLVAPPDLVRQLAAVAAQEGLISSADSWLTYADADADFNANAVGSSSASSSYDSDADGDDGDARGWAAVWSAVCAVWASKWTDRAWLSRRALGIAEGDLFMSVLVQQAVPFQYSFVLHTSNPVTHTAGELLGEVVVGMGETLVGNYPGRALGFTAPPSTNGDGAGNVDMSPKLISLPSKRTALHAPLAGASQTPLLIARSDANAEDLEQYAAAGLYDSVTLQPLLARPPDYSTEPLVDNPDFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPN0</accession>
    <name>A0ABQ5SPN0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable ubiquitin carboxyl-terminal hydrolase MINDY-4</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Probable deubiquitinating enzyme MINDY-4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_017059</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Probable hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MINDY deubiquitinase family. FAM188 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025257">
        <property type="entry name" value="MINDY-3/4_CD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059022">
        <property type="entry name" value="MINDY4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039785">
        <property type="entry name" value="MINY3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12473">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12473:SF8">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26038">
        <property type="entry name" value="Dimer_MINDY4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13898">
        <property type="entry name" value="MINDY-3_4_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01174">
        <property type="entry name" value="DUF4205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Deubiquitinating enzyme MINDY-3/4 conserved" evidence="12">
        <location>
            <begin position="565"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="190"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="435"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="444"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="458"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI71744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="904" mass="94797" checksum="7065C29E8E0C1D68" modified="2025-10-08" version="1">MSYQSSDIVEALVREYLVRAGCTSALEGFNREKPKHANSITKREVLRKSLGLDKASAYYKKQNPTADGLPSTLEVWVIHQMAKLGATSDAVGSALLPAPRPAPGLVRPIPRAATEPDEVSVPMAAPGLPRRPSAGSGIAPAANLSSAAPATASQGAVPAWRRRGLDALRKGSMRSRSKKVLLTPKLAAVAVPGVPHGPSGNPLAPLTPNRSRRSLSRAPSVMPRLAPIPQIGAKAAAPKLSHQSAALMHLPPLRGAVKHRPSSRANALSHVEVQGQPHTHAISGTAGTAESCFHGHKSAGSSMNDNDSPHKAHTVFTSNSCAPALSTITTFTPSTTTISTAAAAATVSTPTSSTSTTTATESDLRMARWTATASSSHEGPPARPVPLRQGFGFGDAVPPRQAAPTTARRISSTPSVENDSDGEAEVIHHGMARASAGGMVRASPQGSMQHSASASNVSLRASAPGLGLAAGSAPQVRSGSGATLAASGALAPPRAHVANRPDGARLGMGVSRHRGEMVMEDVGDINFDDESDGGAAAGVATMRISGPATRKPGTAISPDVLRQVKQLLWGAQGQPPPSWKQGFFFNRHPGLQFGLLQKQGGPCGVLAAVQALVLAALHSPATGFNTTPRGPEQQSALASAITESLWQARMGPTAALVLPEGEAGGAAGRLGYEQLCRAVAQHTATSKEALLDLVRSSLSVLMSEDGWGVVLLVMSLVLSRGVDNVRADMDEPSNSLMGMHGYCTQELVNMIILGVANSNVFDGNKHLDGSTMLKGISRRCRVGLLTLFEWYKYVEVGASLKNPTLPVWVICSESHFTVLFAQDSRALQNQLPFDLYYYDELANQESIIRLSVTKDPRGGWTARVGSSFNDRGKCEGQNIPPLECVIETRWPGVKVNWNGHEAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPU0</accession>
    <name>A0ABQ5SPU0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_016235</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF289">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE VIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="27"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="298"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="590"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="725"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="793"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1128"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="598"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="679"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="793"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="844"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1154"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI71136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1200" mass="125067" checksum="C1D3C0F753EDF8C9" modified="2025-10-08" version="1">MGVFDVKLESPIEDLLVCSDADVGELLELDEVVGKGGFATVWRAAFNGQLCACKVLQPRGGTLPPSPIVRLFIRECMSLMLIEHSAIIRCAGLIRMRMVDFPELGLTYDTYGMLLEFCEGGTLKDLLLAQLVYKQDQYALREALSWLAQVASALQQFHNLEMPIIHRDIKPENILLQVGTPSALPGFWKPSQQQQQAGMPPMRLGCGAGGRSFRPHQNLDAKLADLGLGVRVCEKRSVLIRRRAEPFDDSSATAVADASAGAGDDVALQVMISGVASAAAAAAGCDNVQERVRTPFMGFTTTPVKPPKRLGSNQRKPSNRHSNGAGVDSAGAAAAAAGGVGGSPSGVGGGCAEAPGSPPRADTLHRAILLSNVRARLSGTRESAAAWGASGEQQVLVAAAAAAAGGGGGCSLAGQHHLGEARLSPAAVAGAPVRSSGGGSASAGTMEAGGVGGVVGGGNAPAATAAAAASGYDVDIIGGTRSSADGPSPFRDYLQRQPSKQVPYCPPVLTNSGRTPAGGSAPADAEVLASRADGGGVGSSPFTLSFSQSLGHTMGQGHLEHVLSPISERSISADDTMNLSSDCRPCSETLKGSGVLPDSNTQTSSGTHALQLQTCSSRPPPSLGRREQTQMLQTIQEPSDETGPLLSSVSSAPATAGAHHHRLQLRSDQQSPKPPPRSPTLNRQQLPAEQSSPSRGSRLATAAAASVRQGLRRAAVAAAAVGGVLSPRPSIAGNVPQVDMQDGDAAGPRLDAGAGSGGGGDQDSESPRPAAKFIPTPLPNTDLAAHLESRNLHQLQQPQQQSNQQPDQQLPNQTPHHQPQPQHHQQRHGPAAAHSAADPIIPPTHTVTTTTSSTMNPNNALPGGAASSPTPPLMVATAGYGSRGSRVNTGISVTAAVTAATAASGMPGVMQLPRGETMTASATASATVTGTVTVTLSRGPSKDRVGLMSLSSLVRPTLRELRHSESLTRAMRRVGSLLRSLKLPSLPKFRRNSQFQWVYGLTGQAGSCMYMAPEVFRGEPYNEKVDVHSFGVMMYELLARDLLLVRYIGGTRAGAELGVRDPHQYAKKVCEGFRPPRHPRIPEAQWQLIQACWHPDPLVRPDMAEVAATLEAVLANLDSQQAALHTKELPSADTAQTAHKQKQQTPRGRRSSDGGDFPPSSPLTQQKQKKKTQEEGRVPGGSDAGAYRSTADPTCHCIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ57</accession>
    <name>A0ABQ5SQ57_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_016782</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF38">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLASE 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="5">
        <location>
            <begin position="2533"/>
            <end position="2781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="74"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="412"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="463"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="611"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="678"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="715"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="839"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="926"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1003"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1076"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1227"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1326"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1424"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1545"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1637"/>
            <end position="1656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1748"/>
            <end position="1817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1831"/>
            <end position="1851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2019"/>
            <end position="2052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2411"/>
            <end position="2458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2600"/>
            <end position="2651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2884"/>
            <end position="2916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="715"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="762"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="929"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1091"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1238"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1339"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1424"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1560"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1589"/>
            <end position="1604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1769"/>
            <end position="1779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1808"/>
            <end position="1817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2024"/>
            <end position="2050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="2607"/>
            <end position="2637"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="2916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI71548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2916" mass="306970" checksum="97FC97AA58B39A1A" modified="2025-10-08" version="1" fragment="single">MSKAISYAAQYTKDGHRANQERQEFVPCDEIRYGDRRHARPSYGELQTAFMSPQQHRSSGPDFQAQRKHPQLDPLFPRRAHHSDDGHSHYQGGPFGHPGWRQHSRKWPDHLDQVTQSYRSQEASAAAVAAAARDDYTEFEQRGCFTEGALAASAGQGLWQSQAPDLGKRPPVQLTRADTGHQPQSAEGFIYRMQRGPPAARDHWTASPAASTTQRWSRGNVLGPMEDAGKPVGECRAPAAPQQRSMSWSGTHVRVEAGAWRRSMPNPALPFDPSSMRPHQVTEPTEPGMRYMPAVFSPAGGERPVLLSPTRHPQSLLLPPYQRWPQLQAEAYPACSREAPLGAAEESAIAISTTTVAQFGEEMGSRTHQNRIDVKRMFGRGRQGGKATAYRGEPAAPCEGSLYDRLQNAPLTHDACGSGRPDGSGTAVRSNATDRGPNPNPLHPESSCQPMFLHIAAETVPGPQSTAAGLGSSSAVQNPNLTARGNLEGAPPPVSSAEVKAALTKLPGIAVVAVTLEPGPNLDGLIGDVGLNRYDMTVDEAVGVRPAEALADAEIGTGCADAVRETSGPGLGDAQPLAATIASAGIGAHLECCAAMDSAVCVSAEASMSMRRRDSSGGSGGGASGASHSLLRPEPGSQDPSSGRSRRLNRSSRSPSFGQHVKRSFPKTVFVAEGEDSLWAGGDGQQVDHEGRPEGQPTVPHEREEVGAAMTLQPCSPSEAATSPEQHHRNGLTPSVPVVSQPSLLPPPPPSPRQEQQQEQQQQEEGEEGEEEEGATVLGLGRAACGTEGLSAATFTLSPSGRLVAGKTVTAADSQDHHHHPHFLQGDAWTQGVLRTITPPMSARRTPDTGRGQMCRRAADDDTAASGAAPHRRRMDAAVAPAAAEEAEGREGVWRLSKFRQAKETPAHIVATGRAWVAVNEPVGGAAGTEEEEEEEGLDDAHSPFQVSDRMRKQRCCEGPLADALFERNGGDGRRVSERSENLEVEEAAAIASMPASAIAAAVAPAAEAESDEGHADEDNPLDGPALLAEKQEAMTAAASTLRAARSSALGKDAAPAGHAAPCALLQLLLPPPGSPSAIAGPLPHAASSPGDEAQVGDAAGDGAAGPPAPAAAFARVVGAVDVGSPPLAPTVSRESCSSDVHGLVNYTADIRSHRNERSPLAANMGQMRPRGQQPGACGGVRQALGGSESALEGLSGGCQLGCDASTAKYVELTSRQLILLPHGEVPRARQQQQNPDPDLDDQDQEEQQQQVAAVGPPLGVLEGATCLLGSAGAVAVMLELAKVFSGGPELGSKDVAAADHSPPRAASRTSLNSIDAVAAAAAAAAGTVGERSPPGPDPRYDAAEGDGGDRDPDLMQVGSLGKERRGCLAAAAATTTEAPGTCSLPATAPVVPASLRGPCLRPLPGSCETSHLDLYQRCLMQQQQQQQQQQQQPSTSLPLPLSFPPQPTPDSTRSLLELIRLQTEALLHEGDLNDPSLITAAYSAFYMLNLPLAAMANQRRQHAAGIAGMAGTSAAAATGAGALAGAGTTTAATAAATANAAPAILGTSRRAQPETDGGDGIRHNKDREAGEAGPSKLRKQSRGRQGASKHNGTRPSVGLSQQPAPMHTSGPRAAPIASQPRPSAGLKLAARALPPVPAAQPSQEVQTAPLRQRGVRRTQVAVPPLAPGNGVNQAAAATAVAAPPAVQQRLKRARRPSGAHAGQGLVAAAEAAVGSRAVPARARSARVAAARASLKIITVADAHVRSMDSDNDEMRSSGGSGSSSSGADDADHDVDAADDSGPADALEAPSKRPRRSTGGRVGGGGKITAAGAAEGRVRRVSAAGTNINTAAAAAKPQQKLPPPPNPEPVRSEELLLSPQQRRERVVLPSGSSYPPFKGGKLYHLNGDEFLGEPLHVGKRLPRGLSLYTAGALAVAQRQPQRQGLLSASQRCHHCGGHCLSADCEWCGRRTVCERCHRAHFLSFAYMELLHKCPRCRGCCNCRQCHIRNRQLVPRMSVTWETRQDMARHALKFITGTGNGAAGGSSSATATTATGDAGSNHSSSSSSSSSGGPLGCYLASERVERLAEGFCRLDEQRQRQQQQQRRPLAAEDDENVEYVNLMNQRTLCDLCATSLPAMHRACASCRKEYCLSCCRELRQHCHQQQPKPPRGASTSRGPAGGWACTPLHCPVPGCGQPTRLRTYLPGPMHQVLKKLDQLAAEDWGDTADAGGPPLSPSFWDPALVVVSEAALRPEQFGSEAAAKRAAAAAQAVMEQVRKCMARRDTPHGLAAAGAEVDQGEKGEEMEEESDPVVAAGVPRYREGQELAPGHVVAWGRYVLPQEDVRLASVRRDDGPNKTPRHIFTPSSADMRPSSPRFVSYALLFQSRWRLHEPVVVRACAGSQPQIWTPEHFSRAVREGFAAAVLDGAGARSRGPVLGKGGGKGPKRGRGQSSAAIGGGEDGGNIAHSKSRSGSGSGRSYPLNVIDCADKFRIVEYMEEAQFFELYDMPYDKTKQPQMLKLKDYPPAANFHTVLPRHFKDFVESLPLPFMTRPDEAPLNLATWMAPGAVPTDLGPKAYIAFGTPEECDELLERDSVTKLHIDMADAVNCLNHIRRRKQEVQAAHGPAAGGGGGGGGGGGAGGGGTGSPGSSAGGGGRDLSPTESPYVSPEARCGNELPVLPKYGGAGAVWDIWAPGHDTEELRRYLTAHKSEFVHCGEVITQVEDPVFDQTFFLNQRHRDQLWQQHQVASWHFEQYEHEAVFIPAGCPHQVRNLTSCIKTAVDFVSPEAVGESLAIAARLRLMPRDPFADDYHPANDGHCDRLQGLLIMMSAAVENYRILHPGSYRALPPQPHNIFTVATDNGDSNGNCNGHGVPPLENQAPLAPLPAGVEVAAATLTGLGARAGAGAHVKKRSRNEAGLQSQAVEEPTAAVRKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ65</accession>
    <name>A0ABQ5SQ65_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Starch synthase, chloroplastic/amyloplastic</fullName>
            <ecNumber evidence="6">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_016750</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 6">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03791">
        <property type="entry name" value="GT5_Glycogen_synthase_DULL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02095">
        <property type="entry name" value="glgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825">
        <property type="entry name" value="GRANULE-BOUND STARCH SYNTHASE 1, CHLOROPLASTIC/AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825:SF2">
        <property type="entry name" value="STARCH SYNTHASE 2, CHLOROPLASTIC_AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Starch synthase catalytic" evidence="8">
        <location>
            <begin position="83"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Starch synthase catalytic" evidence="8">
        <location>
            <begin position="236"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="7">
        <location>
            <begin position="417"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI71524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="611" mass="67571" checksum="81563C8B651FAB52" modified="2025-10-08" version="1">MMLRGRTINATNQRFRNAPATVIKHSQAKLKGRPLVRASVAAAPPVITDVPGIETTHDENVHGTSGAVHVETPGLKRATKLRNIIFVTSEVAPWSKTGGLADVLGSLPFALAERGHRVMVVAPKYAPYKGIEDTGVQVTLMGHAEVTYFHQQIRGVDFVFVDHPSYPRPGGLYADAHGVYGDNQFRFTLLSLAALEAPFFLPLPLPPQQLDPPKPAAQQSNGTHSEQDMVEDVPCSKYGQDVVFVANDWHASLVPVYLAAKYRPHGVYRNARCILAIHNLRHQGVFSPSSFATLDLPPQWYGCLEWQYPPHQRQGSWAEEGRSVNHLKAGLITADRIVTVSAGYAEEIQTYLGGWGMEGIIASRSPVLNGVVNGIDTDEWNPATDPHLPAKYDTTNFVEGKSKCKMELQRELGLPVNEHIPLIAFIGRLDPQKGADILLEAAPSLLRHNNVQLVCLGSGNKDLEDGLRWLEGSFRDRARGWVGFNVPLSHRLTAAADILLMPSRFEPCGLNQLYAMRYGTVPVAHKTGGLRDTVIDFNPWNQSGTGWTYTSCDAQGLLHAIGLSLLTYHNHRDDFRKLQLRGMNREASWDQAAQEYEQIISWSVIDAPYCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLU2</accession>
    <name>A0ABQ5RLU2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_000205</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="1762"/>
            <end position="1904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="295"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="351"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="578"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="663"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="845"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1276"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1421"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="588"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="671"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="865"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1285"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1297"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1431"/>
            <end position="1441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI58578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1965" mass="200684" checksum="B0E4EFE5F773C661" modified="2025-10-08" version="1">MGLGGLFGCLCPSIRARARTRERLLNTGETHSDARSAGASLERVNIDFFIRQPKQADSELLAYLVLARDDADATACGAQGTAASEVFSVAFASGSLLRHLGCSSRQDYLARLGAWLVADGELADQFAKLVRCASSLTNGIVVYPLEDGIQLQDGVYNLTLTVKSCLIVDVEGVLSSDGAGQGRPALAVSHKLSRLDDLSAMRQQEMALTTIHAIVTAMLMDGRVVYQNPRSQQYMGNLVNSFTPAPGTSLSALSVCVHPLKRLFGFDPEALQAFEMAMATETEFRRIIKVPKRIKQPQHGTAGPGQVSLGRAASRGRACAQRERPGGSRLVHQASGVDSLVPGPGARVALGLGTRGKSQDTTGTNGPVSALRSARSGLVSREGSVTRISPGSMRIGFGGSSRRGGMTMGGVPSTIASRRGSVLTAHAGAGLMAGGTTSVTPGAAATAAAASGGARRSVRRCTSVMNLGHRAGVANDSGSSRPSSTVTSRTPSFLALTGAGLGGQRQDLRMLKPPSVSPIGPAAAAVVQPVCLNGLKDGQSPPESMATAAVAARSGGAVTWTAGLPLLEAEAGAAGSMSAVARGGPSPERTTGPSSTDVLLDLPTGIMLSGPHGHEGPDSDSQSESESGKSSAGCSITPGTLFSRTRRLLAAASAEPVYISGKGGAEADGTIASTGTSPQFSPRSHSHMRLVHQEQPTGTAAEVAGIDGSASLTNGVTGSAIHQCTRSLTQLSIDGDPSSPRQLLRSARSFAHGNSPGRLSMQPNPNSATDLCKSVDSGASLGLGLGLGLGGGNEESVTERASFVSSRSSRLGRHGSFIMLGGGGSSIGPGALAASASTAFRRSASADPAGLGASGCSSQKGTGTGMLNSSSGNSSFTASPRTSARVRALHHLRSVDHPHVKLRTRSETHSQEPASPPRTASTMVNLVEVTPTFMDANGQEVEAAHHPDSAARQYCSVSSIGTRAHVGSTPALRVPGGLRGCGSGAGREVPTTGSPPALGAAGPLLACVTGMVGSSSSGNPPGFRNSCNLLSSIQEEAIEQRGSKEDEAGAVELGTGITRMTLAPDATWSAGSGVRHRTGSSGNAGGMLEYAELEVSVEQQAEAVSGFNLHSFVLTHLNSSGGGVERPASSHNVPLPSLNLGIASGTIEPEDFSYTSVGGAGSYTSGGSNGFSNSVFRGFGAALADSHQLSASLLAQRLQTPRQFQAAAGGGVLASTSAATAASGGANPLPSAPTVAMTGPSAASSMHDHRITAASAAEGSGVENALVVGAVPAVGGGVTQDGRSRPSQKYQQQALAPDPDVSPVSALPSVPASSAAGRTEMGLSNDGPTGRSLHVSLALPSVASGGGNPAAMSVTSHKPFGSQSSSPLAAAVVGSSGAPDTTGTATSQLAKLLSLPRSRITSAAQLGGGGVDAALRETGIDGNANGRQGHVHGGGGDGGGVFLSAGASRDAGSERHGTVVSDFRLSSEAFEDVSTLGPGRGTTFSTLDDVEQHMLSPSLSPSGQRTSFPLQRDDGAVAATSGTASGIPDHIPLHRTWTSGDKLPPVAAGGGTTTTTAVYSSFVMERTMPEVAVSGLSHAVTCPAPSCATAADSRGRSGGCSIGVPTVTGTASGMMTLTSTGMAQRVPQLRRRSTKDRLMSLLMSLSRDESDDEEPGDEHEWHDVYACVRQEPATGRAVAVITQTEVTEQVEVQRKLEALLEHEHKVLESIFPRHVIEHMTLQAKHRGGAGGGGMGAVSVGSSWANFRGSAMEALATSHSCVTILFCDIIGFTEMCKEVSAMVVMRFLNTLYMKFDELIDIYGVYKVETIGDCYMVAGGLVRTDVEGNKSVIGDGSEDALHAVRVMSFAKAIMREAAEVVLPTGGEPVQLRVGLHSGPVMSGIVGDKMPRFCLFGDTVNTASRMESTCPPGAIHVSTDTRALLQNEAWVATGGVQVKGKGLLETYVWAGHAAEEDEDAERRLKVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLU3</accession>
    <name>A0ABQ5RLU3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012416">
        <property type="entry name" value="CBP60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31713">
        <property type="entry name" value="OS02G0177800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31713:SF96">
        <property type="entry name" value="OS02G0562300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="966" mass="105005" checksum="82455C1E17243F80" modified="2025-10-08" version="1">MQPVPLRDELRTVSRAVLEEYVGARLKQFSKVFASALLPGVTEHAYEFAYSRTLQQLGSSAQETQLTDSYVLRFTHRCRFLGCMRKECPLCKNNPNKKCREDDNFDECYADGQVLKSKCEADVYLELVNLRTGAPEALPGVDIAVSVVDGESYNENASGNYSHLKELLKNDDDSILLGAYQAGVKAESDGRVYLRMNEGQVKLPDLCVTDKNDTFHLNNETFGTFRLLARAVRREGFGVLTPVENIRPAVSGRFIVKTQRALNDYRKSEYPHFKDELTKLKFIGSITAQRLREIHQHLDCPYSSIETVEQLKQLMLYADQNRQVENKMLELLNMKGKHKHKWDYLREVLAERVVYDDMLQRLWYADDTMTQGVIFACKQGQVNMDRPIGLVQRAPNGRLVVSSNPTGPDAEALKQWRSLAENAWHTPGHRGWMMMQEQLEMANASAGGTSILIGAGPSMAMTPTNSISMADGIDTQMLGRRGSVGMTPMIHPDSARSRRSSAVSYHPGPSSPERTGQAMFQQTSSGYADSGMQQQQVQAVGPLQSQQQAPFQFTGAQSLAALNMYSAGLSASELVPDVYRTASAPNMATSSPMFSHNSADSPGGGGSCLMGTVASAPSGAMAALGGPPELTLPPPPARSNRYRRLSADAGASVLKFAMLQAAGVQQPPVELPSDLTGKRSRNSAPGHQMLEELHLQQQQQQQQMGMNADVQITSNNRHALYHFESRGRHTPRSRLSQEMSDTVAAAALSGVLDASDIEGYLAQNFSFVTSGGEAIFSGSGAAAMTLGGADQRGMSGLPPGNGGGVSLGTMSPTGISAPLLQMSMQQSEQLTGQMQHTLILNDPSSRMHMAGSNNSTLLGLETQQAVHHHSHNHQPQQQHSGYGMGLGMGGLGGGMGGSGAPAGSRTLQHMDSGNMLLNQANRELKRCESDSFKRFLAVGLPFSPMETTPEDLFEGLHRDAGQIGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLZ0</accession>
    <name>A0ABQ5RLZ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000356</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="30"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="505"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="750"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="654"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="750"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI58621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="903" mass="96291" checksum="E223E7CED729D29D" modified="2025-10-08" version="1">MPGEIARIVPSQEPGVYYVGRDTWQVGARYELRKVLGTGSFSAVCLAYDNLDDVKVALKRVGDVFASPDNAKRVLREVCIMRRLEHPNIIRLMDVFLKPSPTGKYVLRGGRLVPTSYDCYMAMEYCDQGDLFNMRGQLPEHEVRSLMWQLLGALRYLHGLNVWHRDIKTGNLLLTLQEGRRILKVADFGSARSAKEVNDPGKFVQADSGNGRPELPLAGGDGMDLEEDSNGARRYTALAQASHCSGEGNMGNGAAVERCGSTIHRKSRPSGTCGGSVTSGLVAVPAPDGGYQPPLTSLVCTPCYRAPEVVMSRGGYSSAIDMWSAGCVFGELLQRAAYVGKASTPHLQVAPVFAITGKPLTPHSGQRYGSAEPGGSTSASAELSALFAVIGTPAWACIEAVPSDAWRRYLFHIPGRTSLAGRSLLDLLTDVPLELFGPKAPTLHRRFSQAGEVGVDLLARLLQFDPGRRASAEEAMAHEYFAIMHVEAQMAATVLASQVTGYRRVGSRQYHKSGPPWEGDEETRPLVGSLTASGPGLSSLPVWPPARGPSSGPGSGSGDPGPSAVMEVDMQGGEGGSKAGAAAQRGSVPRTGSLITQPPSGGARGCISLLTKRKQLENSSEEELAERRLTQPPGRDQQQAPRQSEGCPGERRGQQQAAASHGVSQHQSDDRRKLSFWQIDEPALALAALEAELVSLVPPDMPGNSLDAALLTDRARAATVSQTAEALALVEECKERLREMLERECAEHTARATVRRGAEQEMREQRSSDGESAGPGSSTGGCGSLRGNSESLLVGSEVQILGGAAGAAALHGHLRPDPGQQLDPSIIGYERIPYHADAAAARLEPEKHLWAGRHGEWTQSSLAAQRREAASGTWGVTLMPPGLDAGTPAGKAYIDVVRSQQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMC3</accession>
    <name>A0ABQ5RMC3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PSP proline-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000484</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007180">
        <property type="entry name" value="DUF382"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006568">
        <property type="entry name" value="PSP_pro-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052584">
        <property type="entry name" value="U2_snRNP_Complex_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785">
        <property type="entry name" value="SPLICING FACTOR 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785:SF6">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04037">
        <property type="entry name" value="DUF382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04046">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00581">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP proline-rich" evidence="2">
        <location>
            <begin position="345"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="615"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="646" mass="70702" checksum="92E144F5C6B69388" modified="2025-10-08" version="1">MAQEETVHSGTNGGPQTESIANGAKKSKTEKEKERRKKKKLNKQLRRQHGEQIKGAVGGTVATSEDGDDEDIVIEYVSAPLDLEALRIDGGSASTGEADGEEDPDRLGLGLGFGGLGFTRPAEPKPQATDPYEDFKKVFERFASAEEVTGTAEDKDGDEDDRRGKDDKNATTSDQEEDKDSEELEGEGIEGKLSKKKLKLLNRLKVAELKAVCARPEVVEVWDVTAPDPPLLVFLKAYRNTVPVPRHWSQKRKYLQGKRGLEKPPFKLPDFIEATGIAEMRSAYQAKEDAKKMKQKQRERMAPKMGRMDIDYQVLHDAFFKHQKPPPLTGVGELYYEGKEFEARITHCRPGDLSETLQMALGMTEKSPPPWLINMQRYGPPPSYPNLKIPGLNAPIPPGCSFGYHPGGWGKPPVDQEGNPLYGDVFGEHGDGDDSDEEVDKETLWGIMDEVEESSEEEEEEQEEEGGQALDEDAISEGIASGLASGIVSGLASGIASSLPSGIETPDTINLRKDAEMPRQLYTVLEQKQVAVGTTGIMGTDHVYVIPGAGAADKQQPGAGKKKPDFLKHIGADVEVTISPEELEGLDEAQVKALYDAKLAELRAANRREDFSDMVAAKAAQQKRKQQAAQDSKTAKKAKGGDTFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN21</accession>
    <name>A0ABQ5RN21_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_000747</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="356"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI58962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="767" mass="85578" checksum="ED1B960BC326751C" modified="2025-10-08" version="1">MSEQLAVYRRCRRQYPGTAACVLLPAFCRRKAEASLRGMRRVHRFEPGTVLFNGVDREGTLAPWPGTSYSVDVWYEAPDEGAVGYGPALSALTLSAGQSLIDNRVLVVQIGLNGRTLKARVDTGASDDFISVAEVNALGLTPESSDCPQVTLADGGKHPIVGRITLSLSIGPLRVVTRPYVLQGLAETALYIMGSSTLRQYCVCIDMETATLHMCKGTLSIKVPFLSFEHVTPKEGEAEAVPMVNFAVTTMAARIEPELVGRKEAVRLLWEGAHGLLLRPRVGCCATTTQISVDPEVEALLSQYEDVFKDIPGLPPMRSVDHTIPLMPGAQPTARPMYRLSPLELDEVKRQVTDLLSKGMIRPSTPPYSAPILFVGKKDGTLRMCIDYRGLNASTVKNRYPLPRVDDLLDKLKGLAYFSSIDLQQGYNQIHIAASDIPKTAFRTPFGHFEYTVLSFGLTNAPATFQAVMDRMFRPFIDRFVVCYLDDILVYSKTREEHLEHLRLMLDVMRREQLFAKRAKCFWAQSQVEYLGHIVSATGVKMDPRKVAVVHDWPAPQNLTELRKFLGLTNYFRKFIEKYSIIAAPLTNLTRKGVFVPLESWTSQCQRAFEELKGTVAEDVTLAFPDYSLPFRVEVVSDASVFGSGAVLLQEGRPIAFTSKKFSGAEIRYTTGEQELLAVLHALKEWCCYLEGRPFTLKTDHKPLTFLQNVPTLNRRQARWMEYLARFDCTWEYISGKLNIADALSRHPSLHAAILAAPVVLSFLPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNE4</accession>
    <name>A0ABQ5RNE4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000877</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF30">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="37893" checksum="658A843B9F0BA8AB" modified="2025-10-08" version="1">MSLPVQIDWATQLQPHFQAPISSIKSRTIGSLWAGYGSVSSLEVHLKGQSSPVAMVVKDVRPPRGSGVSHERKVASYRVEEHFYKHIAPDLISATDLGLPRPLAIESSPAGFQFVLTDLCKDYPHPARGSLDGQQVRTVLSWLATYHAYFWERPTPSGLQQQGSYWYLDTRRGEFELMDRSWADLKAVAERVDACLKSPGESEFMTCIHGDVKDENILFSADGTRCALYDFQYTGRSYGVRDVVYLFASSVDSSDLDDAEDEYLSYYHSELLSRLALKGEAGIEAGMRYRPDVMRRHVDLALIDYVRFMAGWGIWGSGSDWAVRRAKALLPIVRQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNL6</accession>
    <name>A0ABQ5RNL6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046614068" description="WW domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="128" mass="15240" checksum="2A3DD853F60F05AC" modified="2025-10-08" version="1">MTCRLLWVLWFVLAVAFRLLASEVTAQESAKTVQESTTYYYYNEVTGAVQFEDPGSSPHEDESGKRYWLSPSGERLTEEPNRFQWTWFESWSTEQSRQYFFNQATKESRWDRPPDLAWLRLPIPQEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ97</accession>
    <name>A0ABQ5RQ97_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Obg-like ATPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001650</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="entry name" value="TGS-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF7">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="25"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="309"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="403" mass="44894" checksum="B4AF80D150FBA713" modified="2025-10-08" version="1">MPPKSDAKASEPKGAARFGRVRNSLKMGIVGLPNVGKSSLFNLLTEQNIAAENYPFCTIDPNESRCAVPDERYDWLCNLWKPPSMYPAYLQVTDIAGLVRGAAEGAGLGNAFLSHISAVDGIYHVVRAFDSDEVVHVDDSVDPIRDLDTIQMELCKKDLEFVSKAEEKEIRDVKKSQGMKISLAFTTTFEKVKEMLKENKPVRCGDFNSKEVDLIREKLDNLITTKPVVYLVNLSAADFVRKKNKWLAKIHAWIQEHGGGVLIPFSVEWESKLWATRDDPDGRKAFLDSSGGAVSALPKMVVQGFKELNLIYFFTAGETEVRCWTIQRGTLAPQAAGVIHSDLERGFIKAEVVSYDDFRALATSKSMMEVKAAGKYRQEGKSYVVEDGDIIYFQFNVTSSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQE1</accession>
    <name>A0ABQ5RQE1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">30S ribosomal protein 3, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001751</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chloroplast-specific ribosomal protein cS23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038447">
        <property type="entry name" value="PSRP-3/Ycf65_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006924">
        <property type="entry name" value="Ribosomal_cS23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35108">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35108:SF1">
        <property type="entry name" value="OS04G0461100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04839">
        <property type="entry name" value="PSRP-3_Ycf65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="337" mass="37913" checksum="24896AF562A4E687" modified="2025-10-08" version="1">MASALLASRQVPSCLSSQRRSARSSAVRSRPAPFTPARRVFARAQEAASEPTVTDEELAQLVEEEEDEDEVLDDLDEVYSAAAADEEEGEDDFELDEEDPDLLAGLEAAALLEDDIDLETAAAVGAELDTDEEDKELAAAALEEVLAEKLSGNVMISEAELAEITAGVPAEALEEADLEDFVPAMNSDRYEERPLSADDKEAIAPYKLTKGELRNLLPQDWDQINVDFFSNKKDENIPLPEFRLNVLWTEKNLAVAIDQVYSRGQVSPLTEYYFWPRQDAWEELRMNLEMRPWISERDRIILLNRLTQLINFWQDEEVKHTVEEARAEFPDCAFAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQI7</accession>
    <name>A0ABQ5RQI7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046968695" evidence="1">
        <location>
            <begin position="29"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="329" mass="36469" checksum="445BD695E34F3933" modified="2025-10-08" version="1">MSQTMGLLGTSSLTIFLVILSYSTGIRGAPTIKVVGDRFYHPKSPEDYLQTYVILPKDHTSITPPIAVGVEATHGFVDKASQLPLKSMIIPIGPSKFFNLTSIDVPTTFIPAVGSNPFTFVYWSYHLFGHIPVGIYDKAHFDFHFMLPTADYWWNQQWTPNSHGPCLGANNETFFKTFKPIPDKCWPGGASSKIEQIGDFVWGMGSHLFDYGAPEFNPNQYFNFTHIYGQYDGAIQYFEAMVTQESMQRPRGYRECRPFAKPEAMAVSRDIPGMLCLERKTKVFAVEYQTFSKVPGDCGALTAKNAMGGYPASAAPVPPSCSVPPPLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQW7</accession>
    <name>A0ABQ5RQW7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021838">
        <property type="entry name" value="DUF3431"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11913">
        <property type="entry name" value="DUF3431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045395242" evidence="1">
        <location>
            <begin position="38"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="568" mass="61831" checksum="DBC661D6AB2283FF" modified="2025-10-08" version="1">MVIKHGHQVGGRGRASPKLVIVLLLLLRSQSLQPTLAKQIRKRLDPSYRPRPIWGCQNGLPAGAFHIVIATLLPEPMALVPWIWNLGLSGAEIWIYHRMDLNDPGLTAAAATFASFEPYPCDSRVRLQQLLPNKGREAAVYLSHIVRLYDNLPKCLVLVHDHGPASRHSLCGPFFRRVRGYYAGIREELLLRQQQQHNGGGAGTAAATVNTSALILTTGAGSDGGGRALQSSRAFLRARNGWGSGGGGGGSVTSGGGIASPVRQVLMDFAKQVVSLSSGCQENWLKGCCAQLVCAEEASDDIWMGESGETVSERQLLTTESTVASANSRVLLADAADGSRSSKRTYQGRCPFKTSRCLANTSAVRIPLRPGVQRWLYLHGSGGLYDTRYENLVVLHDMAPEAAAAGAMGSTNVGFGPLSLVRYAGPDASETLRMQTSSSRSLDYPEGAATRTAQETFAQLGRILTDHDFPRRKANGRFKSCCASLMLRPEHIQYWPKTLYEQMLSYTLDEQNSYHAALAISHHGWALWGIGGVRPEDLLRYFEVDLLQLNIRGCPGWRVVMEEVPEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRA1</accession>
    <name>A0ABQ5RRA1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001831</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052993">
        <property type="entry name" value="CFA-57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="377"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="48792" checksum="38ADBDDEC6B4B9C7" modified="2025-10-08" version="1" fragment="single">MAERKVVHMQFQGDNVDGKPEDRAMPPVLVPIMAVGMTSVPGGVCLLGKDTAVYPVGRTLAMYCPSQPSTVRISEPLRAVRGITALTLAPNKKFLAAAETMADGYLPQITLFDATTLERSLTLSGTLSGGTFTFLAFSADSSLLLATGCEEATSSAPPTSSFHLWDWANSTTPRCSGTMDRGEIAIRAALDPRYEANVLVMCTKSLGLFRQSPNGFKRHPVEGLEMARDATLVDLATLSDGRFLVATSSRQVLVVDRTHAHIAFYTDRPVTCMVGLPDGAVAIGTERGYLQLYRRSANSETALYSIYSESRVPETVLSVSLDSQIRAMAVGPGGETLLCATAGGGPTYLFNLAYARTLALSSDPQNDLWEVLLPGCHEQGIISASLAYKRDFLATASKDLTVRVWQTSPLRLVLTHMCHHSPLALSIDPYGREMVVAYVDGVRCYSVVEGLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSL5</accession>
    <name>A0ABQ5RSL5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002287</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0157">Chromophore</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1610"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="626"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1321"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1380"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1501"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1327"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1404"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1514"/>
            <end position="1528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1749" mass="192750" checksum="1AC1810AFEEC1945" modified="2025-10-08" version="1">MMSTRDGDANSQQSGNSGTSGLSETSAQRSDSKFIKRRAPGLHARNEEEADVLEQKRNFQDGVFACMFTLVRQSAMSHWKYAFLKIVLEFLMNFVVAFNPSFKMWHIDRNNPVWQVIRWTVWRSPIMRIYGYKTYIVIMYVMAAAVLLSVVGLAWLTLAMRRQEQSRLLRTASLGLHVVYDIFFVMCYVSFFDYFTFSADCDFLGTKEHMYFEGVECLKMPHLLHLLVALSVGLMFLCVTALVVVASCDLDPVSRGVLASPAAYVRLKILMAKAVYIIFANCLDSNFKVQTIGMVVSVALIVFWNLKSMPFYLNFTNIVWTGLWGGVMLPCIILTILAFGKDKSDTFRHTMTQNVLYGMVPIIWATMVLTGVYVWWAMRPARKFRNLELGVKLSKVYKFQSVYQVERLARVMRKFNADGLVAEEAAALGEVIIKAGLQAFPNAPHLLILYANFVLEVHKDGPAARTQLQLVGKQMPNVVERYQVFCTNEASKRLKGSQEEGMDLQAYIEFKRNIRAVLRVHKEALLQQSELWRLCMRSTLKVAQLDAAMEALDLASARADRVYRRVLERYPTNGKLLRCYGKFLEDVLNDQIAAARAYAGANRVGSSNALLSLDFGGALHNGVSNRKPDFLTSMSMEDDAIMVIDAEGTIMMVSQAVQKAFGYAKSDLEGANVSVLMPQPFSQRHTGYIQRYIDTGEAHVLDTVREVVALHKERYVFPISLCVTKLSGIGTDSIFLGVIRPLPTNLQTVRAWITPNGVLLCADQQFSSAIGMPEEDVVGHLLGSLVTDANAVVKLLESCQAATVVELESGNITTELTFHHCFAEPVPFRVDVCMAGSDAQRILVLNCRRTDGMDGSMLAVDTHMRIKFASCEVSLLLGYSMRKLASMRLDQLLPPPYNALHGKWLRDPPHTSRPGSCRAGSVVHLVSEAGVQVPVRLDMHNLEIEASGSSGTIPLYIVKVKKVTPEEVLREKRLVLTADFTGKILTVSPVDSAVFEFRPLSLLGTNLAESIDIFADWRQRSGETQMQMILLALLGKEQEMPGTSWRVRVHEPQPSVGSEAVAAREIHRISRPACLQVELEEEESSGEMDAETGVRIQVTLWRRDLLGGTLELDEELIIRRASPLTGLITGFPTTAMLRKPLRSFLNIPHMSWEKLVVASSHGHHKRSALKATNDRGSISPVMAFIGPHPDSGTMRLMVQGVETLTPGGRPKITLTVHPDTTFVGAHANIMRVLRLEDSISGVVSIANCSVHGDDDAISHVGRLVRANSAAPSALRSRSFSALTAPQPRLGPSPSGLMLVGSGDVRRAELTCKAGALRAEAVGKDCSSEGDAEGDDFNGAVGDDDDESSDALMAEATSVHRQATSKSEFVEQWVRSITHQASLHQMEHQEQERRSRKQRRSTKNATAVSASTAAAAPGGPLLREESSLLTLPLASSAAADAAIEPWLRTISRLATDTRLKSVAEVGTCGGTEGNVAGAQQAGCSPNVVQERQRRAASRLLSFKGAGPSEPGGGESPRKPPDVVSHRGAETTEICTDGADEAAKSDKGSTLDGSSVDDGSQAASAISSATDASSVSEVVIDARRAKLLKALNKLLMGPVLMKHMDRLRLRSYYLMTLMFLTHLIAYVIITSLIKKQYANVYSVHRQALAMDRSQLVVVRTVLGTYCERANVTEKVAGCIYTLNTSVSRLTKNLLQMEEYHQGVYLGFDTNKIAEPAQNVYAVWTTPTMDYQGRPVNANRSRYRVSSDLWAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSN4</accession>
    <name>A0ABQ5RSN4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002157</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="170"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="490" mass="52510" checksum="58BF5E674247C14C" modified="2025-10-08" version="1">MSGMASFVGGVCQHGAAYSSRSSTSLPCPMEDEYCLSSSTDAIECSEDMSDYAEESSDDASDELYGRQRAGCAHPYESTNRDMDVHLGALIRADLGSQQEIYPCDKHVFYRAIYAASQSNSCAWPCVAVSGASQITDTRRKQAHCSPTLAERVYSGWPESSLADRRLLVAWMSELARAAQLQRETLFLATSLLDRYMAANVASTTEPPERLLQLLAMACVSIAMKFEEVVPLHGADLVGLAVDDVTGAPLYQMSDLGHMEWRLLWMVNWRARAPTSLSFLQYFLYCCGSCTGASGAVSPSDTLDWASLYPADGVGGAAIAFAAADVVSAPKGSLRRKKGGAPAAASPGAAANGLPDSLETWAHRILDVALLHHCDLVHCQSTVAMASLVLAERTAAAAAVATEVVVRRPLRPPPLVPYLIRAGAVPVVSPYGPCSSAVQTGTGLSLDELAPNLEPCLDLLERAWMAEQQQQQQWGLLQPRVVLLQPPPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSW8</accession>
    <name>A0ABQ5RSW8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_002895</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="591"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1083"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1083"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1094"/>
            <end position="1103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI60691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1103" mass="122561" checksum="1219E529CAD5C038" modified="2025-10-08" version="1">MNMGHLDGASSRALSRCPLRSIQKLSQSVAAHQSGVTWPPMTRRSAWPRISSNTNVGRSRWGTRFAAAAATSSAPAESSSPTPSSSRGSQPNTQQQLPPQSFRLDEDAALLSNFHRIDASARNDPSAPGSIFSERFITDPRELHLVGAGLPIIDGPEWPDQYWRALQAMSAGRPVELPRPHPKFTQQDLGRIAASEAGPQLDDLVLEHVERLTDQPLDLQLLAAQQRAALQQQRLEAGQLLGRLQGPEDVDPEVVAAVRDATPDGVAHAFRYIPRSVLGDYRTEVEPDWRSMPQMSVVQLYNGLRQRNWTSSHYRPDAEPWRVQFFTCDYLQDGVTGWTGWRAVVTSKGGVRQWVDMPEEGELVQLEEQAPPADLMDLGYRHVFAQLYQAFEARYPPDLVAAIYSSGGRVGRELAASAAAAGGLPHETPALRHLDVSYHSTGNPVQDRLTAWLFRPSLDGLGRAIQWGLDHSGLHELRQLRDRLLPGWKPPTLRLNRSNNNLGVVYFAVCLTLGIVIPALRRSRILDIRTLEEDPGAAMEFARSKSEARKEGLTGVEFADVAGLGPILSEVVEVVEFLRDPKTFSKLGARPPKGILLEGDPGTGKTLLAKALAGEAMVPFYQMSGTEFTEGIVGLGAARVRDLFKRARATAPCVIFVDEIDALGLRRAENDSAKTNEEREQTLNQLLTEMDGFTPDTGVVFLGATNRADLLDPALMRPGRFDRKIRMPKPDTEGRYEILKLHLRNKQLAPDVDLLQLARDLPGLVGADLANIVNEAALAAVRDKGRTVITAKDMYAGVDRFTQGEVRPALPTTHRLPILCFAAKEIGIALVASVLRERHGRVEAVERVSIQPKGRAYSRTMFQRGTDEEYHVMTRGRMMDRIRVSLAGGYAVRLALGEETNFTAADIKRAYRMAKKFVFYYGFSDLGITTWANQPYSSDFLVGQQRARKVVSTDAMDGFADWPTISEDFRFDAPSPSDVTWHRYTDEVRRVIKSCSEDVLAILEENKEAMWAGIKALADRKELLGPELRDIFDQHSPRKLTEEQQPRLDMTIYTDGDNSKWPYGIEWLNDAYPVPYWVRKADEEERQRAEGAREPAAAGTGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTI0</accession>
    <name>A0ABQ5RTI0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="10744" checksum="EB53E7AC1DC4E9E2" modified="2025-10-08" version="1" fragment="single">YSVVVTNTGPNHVRMEVTVQAPQGAVALGGRPALWISSTEVLPYNANKLPLFYFYFPHPAGESRQPPLPDVLSGGDAENNAGSDNEGSGGRPMWFDAFPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTP4</accession>
    <name>A0ABQ5RTP4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002761</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="114" mass="12429" checksum="993CFCFDFE6DD6ED" modified="2025-10-08" version="1">MVRQYSSDLAGSQHILGPGYSKQFHSPALPAVGNNRAQELAEGDRRISIRSVALAEGRTTSSKRYGKGYATCCGGLRLIVAQQQQNVLAQQEWPGLGWHGRALGLPWSLRVKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU74</accession>
    <name>A0ABQ5RU74_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002851</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat cdt2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051865">
        <property type="entry name" value="WD-repeat_CDT2_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22852:SF0">
        <property type="entry name" value="DENTICLELESS PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22852">
        <property type="entry name" value="LETHAL 2 DENTICLELESS PROTEIN RETINOIC ACID-REGULATED NUCLEAR MATRIX-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="115"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="631" mass="66741" checksum="73C8D8B42142D394" modified="2025-10-08" version="1" fragment="single">MPPLGQSLAKGLLNRESGRSNSLGYGRGVNQQKQQAELLSKLEFEAKSTLIPLPPATVTSGLPLVAKFNRNPSFNNCQLLAVGGEEGVVTITSTESVSTLEKGALDGSWRPRAHWSCHRNSIFDLAWAKSDSVMYTASGHQHVGVWDTDTATALAVAEGHEGSVKCISPLHLCEDVFVTGARDGRIMLYDARYPGFSSQSVGRRVHAGTDLDNAPVLSPIWVQQNAHTPYTASRGRFSTKVAKPVAVTVTSVVFLPYSYLLASAGANDTVIKLWDLRMHGLPMCDLELPKSNALVGAGRLVGSKDSSTGGGASGIPHEVAEFTCPARTSAVGVTHLTVSETGDRLLVSCLDSQHHLFALSQLLSGPLATWCAPPPAVKSSFYMRSAFSPCGDYILSGSRDCGAHIWMAANPQRPPITLPHEQEVTGVAWCRGEWNKLATCSDSGEVRVWTLNRLPARETWPVEAVTSAMASHVAELDYSGYGNAEIEQSQKTQTAAAAGLSFPLSRPEDADCARRGTTKPIIKRTVQLRLNLQPVPATQLARTTCAAVAEPANTNAPEAGSVQPEHIVPLLTVIEEPAAAKHAALAHIAALTKDVVLLAPGGGTPCLESKLAPACATAAPGSAPSSPIPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUF0</accession>
    <name>A0ABQ5RUF0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="160" mass="16309" checksum="DE3B959E1D5FCC83" modified="2025-10-08" version="1" fragment="single">MLTIVGLHARCAAWEFAALRAATTSSIGASGSTGKPVRLIDGVLDRIVDRLTRGEFVPGQDPMASAAGSAGSAGEAGGGERPVPRAAEAGVGGSGFVMYDYEAVPRSIDIDAFRRALTSVLTEHMGAAGGGAVMAHDGGEITRRLMVASPSERQAIAEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUL8</accession>
    <name>A0ABQ5RUL8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chromatin assembly factor 1 subunit A dimerization domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_003659</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022043">
        <property type="entry name" value="CAF1A_DD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15272:SF0">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15272">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT A CAF-1 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12253">
        <property type="entry name" value="CAF1A_dimeriz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromatin assembly factor 1 subunit A dimerization" evidence="6">
        <location>
            <begin position="878"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="164"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="547"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="610"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="691"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="730"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="891"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="918"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1515"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1719"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1837"/>
            <end position="1861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="192"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="617"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="697"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="730"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="919"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="967"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1548"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1748"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1844"/>
            <end position="1856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2227" mass="229489" checksum="832C8BF15B10C42F" modified="2025-10-08" version="1">MAGIMKFFKPTEGVGRKADQGSVGTAAREVFSPVCNTKGVQRAAIRPALDNDSDVDIVDGEASMGLKRKRKVSGHTTSGQHAPQIHCAGAVETSQTCQSSGLSGHHNDFFAAKPGVAVVADVQMAGLDQGLKASGPAAGTKALQQAMAAATTVAVPEFSPDLAARQPTAKRQRVPEVAPETSSADNNPLPMQQQEQQPHQQQTPTDDQGAKLYHWESQAAAPSDVMAHDNLASAPQKVAALQLQDAVRMLASGSSPQAASTVQLPLVEVLHEQMPAQLVHEQQRPLQQHHEHQDDTANAFQQEAQQSVADTSAVAMDADASASAAGGGGAVAAADTGGCVGAAVGAVCGGDGGVTGGAAAKSCGAGSSTLPSHLDLAVILESLRHELAGLEAALEVEMHLPESDSGLSAAVVASEGGRLKSMPTLAQLCVWLEGRTAPLSGLVAYLEPLLPVGADLAALRTSLVDLAQRKCYSAKEAPLEGAAASEDATPGRLWVWEVREPRKHLQGDSRDASTRKLAEAMRKRRKELRERLVAVVAAIEALSSHQALTQPPPLTTPGGPGPAVKGPSAAARRGSESRAAKAYNRLSRLADLEAVEAVYRSTTEALATAGEAAAASARKERARKEMEREKAREEKARQREEEAKAKEAKRAHDDDAKRRAREAAVANKAGYHSNKELEKSRNIMQSFFKRPLQSKPTGGGPTGQSGGDAAAAAESDGLAVATDAAGVSTGAATVSGTAPPTSTSSHLPLQDQAAPSGGGRSCSQPAGATPARERAATRMPFDFSKLARRVPDKTVVEALDDDATRLPLPAEVLAGDLRTNHARWRQQRAARKRMIGVPPTWARRPSAPTDLRTLAAQYYGDVAALGLAPEGLRTWRRKLIAFPRKETVRPPYYGSFSRTSTSVTGRRPFGRDSALDYEVASDEEWEEEPEGESLSDSGDEADSQAGGAAGEGGDDEDDGFIVGDDHLSDDEGAQLSGDPDDPMPVDGAAVKGAATRSAGVVTAARFMDPRLQQLDAAIDRVRTNNRPILLVRPMPAAAAVAPPSGPDVGTDAAPPLRLHCRLRAEPRTGLNTGLDPGLLGALRPVVHHRVGGDASTGLPVVALAPPLELDVYSAYAVAAAGGMAHSLAGAEDALHASTTSGGCEGLHPSAAQVGLLAANGLQAAAPGSARGLGSGARARSSNPCLAPATLSALAEFMSRCGDQKADRIVEGFLATLPPGVKAPAKTKLREAVKRLGAWSASSKRWLLYGGSVAAAVEHALAVGSVPAGAAAVAGPGLGSTAVQPCTPLATATAVTTGAAVAAAAAAAALAAGRGFSADVATTLTPTHGSSFLPGHYSLLAALVPGTIPGPESAPLLHKHQQPNLHGLPPGGLWGPPWPFPGDPGMPALRKIDGGGEAMDCDEPRPPPQQQQMLLLQQHQRLQDHFTQAPEVVVHVLSRPNLPGGGTAAAAFPTGLPASVGNNILSNTIADAPAAIVGTSAGVPLAEAPRVTLAAVGAGDAVVASDGLAVLAELPGGPSEERQHQPLFEPCGPQGSAGTQAVAAAHGQESGGDGSGGEGAYTGRCVTAAAETETEACRVLLMEVEVEARAPEEALQTDAGQHCHTLLRPMPPRPPPLSELALLGSGHPFWSVISSWVMGGGGPRGRSHRHRPSWADVSSALTVFEAGQLEPRLQAVPLDLVRVLVAVCQHSMASHLRSGAFKALGNLAAALAAAARAGGSAGMAATSGGPPGRGRGIGDISETPEAPAGSYSCGGSSSSGPEASSMVGPVRPTGQPCRCSPQPVTLQALWSLPGLLEVLRRGCCDSDPDSETDKAARSAARVLAALVGVGITVPSASGREAQEPEQAAGWQQQLHQQGEGGGGAEQLEVWAVRQRLHALAPEMAASAAAFRLRVVNNGDLHAALRNTVAHGPGKSPSSARNVFHAHMLLALQRLLRDPAAAAQLLKPHVRLAKEAAAAAKGRVPPPPVSPDLESKWAAKLMQNLVTGGRGMVHPGCRKGMVQCAAALMDLPALTVVIPATSWRSNKAQVDEGGGNIATAAARDPRGGNPDGKVGLGDGGSATLRGWSSLLSGNDPRTHLPTAVLTLLVSELRQVSEAVAAKSCADVGKGQDEGSKLVPERQIWRMGTGPLNVNANDSGFGVGAPREDFPHLDKCTRGRVVSAMQLAVRALRHLGRPSSRMQLRQEDTKAAVADVLMALQEAGEVLGAHYDPQLQELYGRVGSLAEDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV39</accession>
    <name>A0ABQ5RV39_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lysosomal Pro-X carboxypeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_003469</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.980">
        <property type="entry name" value="Serine carboxypeptidase S28, SKS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042269">
        <property type="entry name" value="Ser_carbopepase_S28_SKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF38">
        <property type="entry name" value="LYSOSOMAL PRO-X CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045951462" description="Lysosomal Pro-X carboxypeptidase" evidence="7">
        <location>
            <begin position="33"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="569"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="65369" checksum="9F95FF6635621E92" modified="2025-10-08" version="1">MAPASSREAPSSSHWLTTVCAIAILHLSLCLGKTSNTNSKLLRFNGKPTTLRNDDVWSIGCLQESWYSQRLDHFHRQRGGDDPTFPQRYFICDKFWSQNPSGSRGPVFFYAGNEADVTLYVNATGLIWEHAEEFGALVLFVEHRYYGKSQPFGPDSWSSDPSYLSVEQAMADYATLLWHVLPQMGAEDSPIIAFGGSYGGMLAAWLRLKYPHLIAGAVAASAPVGAFPGVPGWEPSRFWQVVTYDATASAGAAAECSSNVRAAFRHVLTLGRTVEGRKALGRLLRLCEAVQDEAAVLDVAYWLQGAFDAFAMGNYPYPSSYISGDPEYPLPAWPMRAACNHLASNRLEPSDLVEALRDAGGVLYNVTGQVQCYNVQATGPAAGSIGKGVSEPCDPSSQGSRCKVPVNSHHVGLWAAGHGLPGPEWGPWDYQWCTELMGQELPYYPTNGVSDMFWDQGAFDLNAINAHCQDAWGVTPWADWSALTYGGLDYQYASNIVFSNGLYDPWSVYGVLHNVSNSVVAIIIPEGAHHLDLMYANPADPPSVRAARQVEMRHVRRWIDQFYEMRQKRPNSTVPGQGRDTSAGADASMAESATASVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV47</accession>
    <name>A0ABQ5RV47_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="190" mass="20261" checksum="FCB60C05EE596DA2" modified="2025-10-08" version="1" fragment="single">MAAVDADVTSPEILRLREEYKALASQLPAALHCGNPERLINYIGCIFNESDAAVAPGISKAGSGTMLNNTHDTPAAPDGSKTDSDACPSKPDSPTKRLTLDQVSKGLELIEQLDHEVQISKDTVTTASSELGWLGVVTSALARQRDSVALTRCPECRRIILACRMPFHRPHCVPAPVPSNISKPPSGAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVI9</accession>
    <name>A0ABQ5RVI9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Timeless N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004068</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF4">
        <property type="entry name" value="PROTEIN TIMELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Timeless N-terminal" evidence="5">
        <location>
            <begin position="87"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="290"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="547"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="718"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="924"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1006"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1220"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1376"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1569"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="352"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="547"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="751"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="788"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="924"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1006"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1175"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1602" mass="173077" checksum="07B6A54F4F6FCD84" modified="2025-10-08" version="1" fragment="single">MAYGLNPAGFARELRESAPGVNDRDDAADVVMIDLDEDSEEDVEDDDGGNLRAAEPQRDPVDYELLISVAHGLGRWMQDSQTGQKIYVKDQDCLGCLKDLQRFLRRDDPDIRPVFKRLGEWRIAAHDVVPLLVTYPHDKEITLQAVKVLTYLTLPVDPSTAQPKVQEGYVRDVKEAMLQQDALVAVLGLVAEPLAAHPRMSLEQQGAVQLVMVLLRNLLAVPDEQPSPTLAAGSSRTRLQASLLSLLFSESIMELLLVVAQHGNQGPLRKEMPTVLEILCDTYRDITPQQLQAAKPPPPPRPQKRRQQQPPPPQPSVPATAAATAPGGGGMGRTPPAAAVTRPSPAAFHPTGASGRGAAAAGRPAGPGGGAAIGLPPPRALDSAVAAQLRRQQLANAAKLRNQPGAAAVLSIRPGSSAGRFIIAHADDPGQRRTFLARPTEGPTAAATVKQHIVEAPPKLDGPRAQGSSSAASCAPQGDAALVWKLRCFADDLLESGYNQVMEVIRREVSAGVGVGRLERPDLLRFMRLAAFFTQYCRLQQEARAAAQTQNPTAAPAAAQPSAAAEPAPAAGGNPPPSPSPSSPFSGISGTMGWDTFHLVCKLWVEQADMSNKLKDWELQAVSMRLLCEMLAVLVVAHRHGTREDRQAADRLQRRLLHDDMQESGLLPVLSRLITSFVPSRQSRSYACDLIMSIHYVLRMYDRLTSLEPGGFMVRAKKRSGGFRKQQQQQQQQHAEEELEETGKGGKGGGDDEGEKEDEEGGVSQEEKSAGGKPGRRSGGSGKAPIDELAEGSDMEDDEDGDDDRRGRGVVERPLDLASKVRGKLAQPAVIYFTVQLLACYKTLPAEVPRAIASLLYRIVAKEHLDLEPLLYQISVLRVFYVLLSDPELRTPAKLPAYREVLLLATRVVRNLFRKLAPERYHREAEGKYTGKEEEGGAEGGPGGGTAEQETKRRQKRRQELVEEIHDKAPRMLLVDMLFWKDHTAAAAINQDYWLWMALEDREREEAEAHASRGPDGGREAIGDGSRRRHGRRDAWGDDKEGDEDVGAGGRNSRSPGADSPVEGRKGGEEEPCTAGRLRRPGGLWNGEAHGDDNGDLADADDNQGRGGPERAGLLQPMSAMRRRTTLGLGEEEDDEHEGRAAGRGGPPQLGPRSQKKARGTVFLSDEEDGAARGVAGGAKGSTGGATADAAGGGAARKALTLQEREEMLRKRFMKTVRVGNGRNKNSGRMGGDDGAEGGGGGGRGVRLSSAQEALLLQVFERHNCSRHNADLILQIMKRGGATGGVDGEPAAGDAANAAGVEMSQGQLMRQLKKMELRFKQLTTNQMARLTALHNRYHEEPDCLLMIAAQLPGGWTSKDVKRLLVKHGFTKVCRRRRHGHGSDSGSSSGSESEDDLDAAALDEEELAELWEEHGGQPNAAEKIAAALVTPAPANVVAAKLRQMGLLKRGADKEAKGKKRGGGREGGGLVDVVALRRLHEEHKNRPDYLNVIAALLPGGKTVKQVQRLLRSHRLLDDDATRRKKTRDAAERTRLVALYQQTRGEPDQLAAIARMMPDLGGGAKQVAKLLRKHGLTEWPRRRSRRNSSGDGEEEGASAGDVENG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVS0</accession>
    <name>A0ABQ5RVS0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="673" mass="71302" checksum="5953DEFCAE844D2B" modified="2025-10-08" version="1">MLSMQIIAEAFRLWLLEEGCAAEYLSLQQLFALCHAGLLSRRKFRSPGTTHGYDFRRETTEDPPGTASPLGGMLVRLLNGCFAAAAALPEPALRRIAASNIIAAFCRSALREARMRGRCDVQAPEAIGSFPSDSLNAWDGLHTGGCGCSCNSDEGLRSNGLAGAGSSVLRLREQDGVAPGCNGVPSIQSSIGILDVAFLDAAFSVLLVAVPRHPLLVSELQVMEVVWSALGGPFAPNGKVESVHPTEETVAAVSPTRATQAGAGAPLRRRQSSEPSGSSEVAGASGRSSVWQGSVDNIFKPAARAADQRPWDATTDAASPPGIPDAKGLAEFFFSPDFQCLLAVGQLLYGTPHAELESSCGGDWQGDAGLLRVTKPHDPLAWDIDFVPVQPAHVHLGAPREEKGKGRGDVAWQSTTGWGHQEGERVDSRRGKTHEAAGNSEGEEPGQSHQEAKWQLLVEELCEAPTLAQLCGAAAVMDIWSDLMVCAAERGEEAAARWLGRMAEVGAEGRPAPNGGNSNTIADTFACRTCGLGVDGMNVGGEMGPWGLRCGVEDTNADASVAHRATSQRCCKLLAAALRRQPETLLSSLPPQLLCVLAVRSSEVRHALLTGIATTLIPQPASTPVSRPGGEAMVTDPWVSDSQPCVQMEQHQQVVSRLLEWFRVVGWDLGEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVY6</accession>
    <name>A0ABQ5RVY6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004286</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024862">
        <property type="entry name" value="TRPV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582:SF2">
        <property type="entry name" value="INACTIVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="760"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="794"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="973"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1061"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1087"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1128"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1191"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="8">
        <location>
            <begin position="994"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1284"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1433"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1285"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1462" mass="158332" checksum="1D1158F8EA0C2048" modified="2025-10-08" version="1" fragment="single">MSNLSRQPSLTPHVRSIAAIAEAEERSGPPTPTEDAGLPGGIYTPTRNAPGDVLPPRARKDSLSSTPSNTESIEIPYRFTLVDLNTRSYFLVHDAPDATTHGASLLVTRLRGALRQAVVLANVEVCLIRNCQLAGQPVTAPVGIDLTCSSPAQLPEPAAERIQRATENARALAPVADLLSKHMHFCTLRDVRGLLEERTQEADQILTEPGCAEGLGEVEEAVLHELAFTFVAYQPAVLDEELKGSQSPYDPRSHTRTIADVRALVEGSARAAGTTAPESLAKLAMRLLDLLRLRKPISPLAGWLLQGAFMSGNVELVSKLLARRSTSGLFELDIIRRLRAAWLGYTRYRPLQVGRSGAKAQTIDWLDFKCSCARAVLIAHKIIETLILRNDKDLTQSVQMAAEAADPLPPPPPLPPAGDPSLSLLVIAAAAEANAGGGGAEAEPPQQSCPEIRLERKAVVIALPPAAGPRLSVSCDMADVTPRRLLDDDSDHVELEPAKWALVKELFPRAAACKPLALAMLHIVLAGKREVQREARAAAAAATAVSSISAATELPLPYTDKEDAGGASLREEEDEEEVAEAEAAERARIEEDAHTVRYRKLHLLAVQACHLAVHSGSLSTVSDAVACIRAVRAWPSIDMAMLNDLAEAFPHQCAALLSAIPLQEVDLEDNVLPFALPPGFTTVAVSDKDSHEFRQGGGTGSTQDAEHFATQRETDASIEASLLLLPRTWLALLFLFWPVEFLSPTTRQLLRVLRCAVAAFLWQGPGSCAVAAAFTVLLLVAGAATRSVVAVARILYYGILYRIWAFVTAHFPAIRQAVYRIRGRRAAVGPVPHAPTGIGQRLATMHRSPNGGAVAGAAAGNSDGEEDGEEGFTRQLGLGIWRTFFAHGHNALIDWAVGWGKGSASISCRKLPMPMVYTSQSGTSLSPGGQRLHQLDTALLEKLLKTPGLHPSVFGSKVLRALILFKWNYFTKYFIILQLLLHITYMGIFLAYAFSIKDMEPVDGRESGPGANTPGCQLQAPTPKQTGLLIVLGVMTVDFLVQELRQIRHFGWLFFTHSWDLLDAFSVALVVASVTLHFSCTSDISPLTLRGLASVQIVLLFMRLLYYAMASDKLGSFVRMVLETTYDLVMFFAFLSVVFVGFALAIIVSQGALADEEQTFIKLFTMMYGDFDVNFLSTLDSGALGLDALTRVLASIYMILVTIILLNLLISVISESYERIRENEKWESLRNKALLVVESETQLWQFFLTWLYTTLTPPSQRHGHDSGKRYLYIIAPEYARRLEADGDEDDEDEDEAGDEDEDDDGQWNGRLGEMKRHITTQARATIDVLRREVCKIQTLVEGVGMAAAAAATAASIAPGVEVEVSGGDKTGAAVRDLASANNAAQLSPGEDSGPTALLDERIVQLLVEQVSKEIGALVSKEVGALRNELRAEIAKSGPAQQKQQGHGGRLTPLPNGTSIRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXQ7</accession>
    <name>A0ABQ5RXQ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Matrin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_004790</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051421">
        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025086">
        <property type="entry name" value="SDE2/SF3A3_SAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031774">
        <property type="entry name" value="SF3A3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024598">
        <property type="entry name" value="SF3a60/Prp9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021966">
        <property type="entry name" value="SF3a60_bindingd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786:SF2">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13297">
        <property type="entry name" value="SDE2_2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16837">
        <property type="entry name" value="SF3A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12108">
        <property type="entry name" value="SF3a60_bindingd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11931">
        <property type="entry name" value="SF3a60_Prp9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Splicing factor SF3a60 binding" evidence="8">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="SF3A3" evidence="10">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="SDE2/SF3A3 SAP" evidence="9">
        <location>
            <begin position="277"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Splicing factor SF3a60 /Prp9 subunit C-terminal" evidence="7">
        <location>
            <begin position="442"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="404"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI62199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="64324" checksum="AF1901F42AFB1B4E" modified="2025-10-08" version="1">MAETLLEQTRALHEDMERYERIIVKDLKQETKSHKDKLMQSHRVRKRLDQIQDAARKLMKIYEDEDKARKEEIEALGAEDKVFSKFYDRLKEIREYHRKFLSNDLTEAEDDTPLLKEEPHVEFTGEEGLGRYLDLHDLYMRFLNLRSQQQRGKGGKVGKLPATTDDGDGTVARVGPDGIEIAEEGAGAETEKPLEYYEYLTSFSDFSGVARKLKMGKQYRAHLNLRYPDYTVQRSDYLEALLGYLCYFYERTQPLAQLQRTLNTMENDFASRWDQGQIAGWEDRGLGNVAPSTSGIELEPFSSAEELEILGADRLKEALSSLGLKCGGTTKERAARLWLTRDTPLSQLDGKHFAQGVVPPSASDAATNSKQAAHARNVARLEVKIRKMAELLVNVIADTKGKVEKKQSQTYEEMQAELAEAEAEVALPEDEEEDDFVYNPLKLPLGWDGKPIPYWLYKLHGLNQEFKCEICGNYSYWGRRAYEKHFKEYRHLNGMRALGIPNNKMFYEVTKMEDALQLWKSIQEKAKGDFKPEDEEFEDAQGNVYSKKTYDDLKRQGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYJ4</accession>
    <name>A0ABQ5RYJ4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="106" mass="11006" checksum="BCC900EF20BF4F5D" modified="2025-10-08" version="1" fragment="single">VNGSGGDIVLTSGPANGLRHDHILEAQPLMPPMHLLDAAPISVWTPSPSLFSPSGAIAHLEALPSLKSLPLPLLSPPGPFLDLNLIRRMYGFLPGSPPPGLTSPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0F4</accession>
    <name>A0ABQ5S0F4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007347">
        <property type="entry name" value="SpoVS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35331">
        <property type="entry name" value="STAGE V SPORULATION PROTEIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35331:SF1">
        <property type="entry name" value="STAGE V SPORULATION PROTEIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04232">
        <property type="entry name" value="SpoVS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="290" mass="31221" checksum="41014840A2168E50" modified="2025-10-08" version="1">MTALFCMNRRPLSSCRPSRSDVSVRFNGTLCLRPCLAQNASRISIWNMHAKGSIVAATKLPKARLVVMQSVKEDVELAAEVPDKPVEPASPKDIKIKVAANGKVQAIAGKIAHSIREGVLPDVACLGPKCTNVAVKAVAISRGYLAAESKEISFQPVYVELDRMEDEKPLRGLVLKLATTSNGQALASPSVDAIEMHVRATSIPTRSAGALAARVREQRAVFLTAIGAGAVHTAVSVIEKTRKYLKESGKDIRALPEFIKVTKEDRVVNAIKFNIVVEDLPVEEANAPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Y7</accession>
    <name>A0ABQ5S0Y7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029711">
        <property type="entry name" value="Haus7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010604">
        <property type="entry name" value="Plant_AUG7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14352">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14352:SF2">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06694">
        <property type="entry name" value="Plant_NMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="429" mass="45616" checksum="9C7A0396C15321E8" modified="2025-10-08" version="1" fragment="single">CNNLMNMSTQQEQLTRLQAVLSQLSASPNSTAILYPGPERYSIIIFLLQKLLSPEIVDALQQRISDAELPGGLSAEEAQAHRIVRMLQCIDFQSPVDHVRGCTSLEESMRMLATLANLALAHQQCTREAAIVAAAAITNTSTNGRAPSPGPNLAASSSSTPRHRTPTRGSSLVGSGGTGGAAQQDANGGTSGGGASGRLNLSSLEGEIRLLDGLAERLPQLMEPGGGGNWFPGDIMAALQTYLQNQPQQQDLAAAVEQRTLKLRVDLPALRAEESRLAAEVTVAGEAEWRQHAEATRVELANLLETAAAFYHTFQTGLGVWCAASRSTDTCGLGPLASELLQRYDTLRRLGADLARIRQAHTAIVSCAPPLTLLEQHTLSQLVRAGHTAVEQLQERIAVEQRVLGETEVATAMVAQMASVAQEVARAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S151</accession>
    <name>A0ABQ5S151_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006438</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039034">
        <property type="entry name" value="INPP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12187">
        <property type="entry name" value="AGAP000124-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12187:SF11">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,4-BISPHOSPHATE 4-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="538"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="552"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="593"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1079" mass="114434" checksum="8FE5322D95673C95" modified="2025-10-08" version="1">MATLQPSGHSPQLVKRASSERSSRSSSRQVAPFRSSKYDKGNILIDTWVNNIKEPALLPMALKPDGAARDDILPTSAEVAAPNVEVGFSRDLSTHVSSDDVPNASYDSADHATLLRKFAVHATSSRRLQGLADAGLMLLALLPTVFLRLIRAAMGLLGITEHRWRGISMVVPLTLEDRTQLEVIVHNLVAGRLGRRPEPPLRGPVSPAQQLFLDVHAILAAASATASAFSLQYDGVAEAALQAKLASYLRSVAADECVCTQDCVKKMRKMARKTLTLLCAAAPTQPAPNAASSSRTAAALARLRFLPSATHMATRPRARAVAKAFMAACLLEQSRSQPDTLIQLVARLPDGDGDLVETMSLVPLAPGAEYLRLLGAAAGDPQRFAEQLEAAVDFGRLRPSDAKKDPAMQGIAINARVHSVGVVRYSALTAAVVAAAAPAVDGTAAAALVLLPGQRQPLNGNMCMSQIESSDQLPLLEQQQQRVQVQEDMAFQEGNLQDMAPGEVDKRVNFRVASTATALAEAVNSAVPACAGPANMRQEKAATAWDDDDLKENEVEGRGSGGRRDGATSERVSVCSEGEGNDTEELLQDVRDATSSQPDLTEPRLSNPTGSKKTLGLMLGLAATASKRAIGIGSPNLSRKATRIAGTVTAMEPGVREVAVACDAAAAYAAVRINGNGSAAVPRSASLQQQSLHAKPPSFTAASGPSTCITLYPTAALDVFRTIYACPRSGAFAVHGREPRGVGLSRLTAIYSACHAMPAVEQSALLAKLGYAHLEGLRAEVELRKALCVAQALPVVIASLRLMCGSAACLRAAHRTGSFLHVQQSLLSDLHRHERAYLEDTKGALDVLADRMVVRFVEGSEKVLPLHVSGSSITVSLPAAGLSAAALAEAGLSRASFCGVTYGLTTLLFNQGINSLQSLGFLTWTGSAELLQDSLNSHSLERLNEYAQRANQSGGPHLAALNRHFSYEGRRPPKDTAMFSLVRCACEEVGGGRAVNCRSGKDRTALEISKALTEEVVVAGLFSASETSWLEGQFLRGLSYVTTSQNHGQPPAYAFNEMEIVTLPPGWRPDWRLCGKVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2D8</accession>
    <name>A0ABQ5S2D8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CREG-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007226</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055343">
        <property type="entry name" value="CREG_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13343:SF17">
        <property type="entry name" value="CELLULAR REPRESSOR OF E1A-STIMULATED GENES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13343">
        <property type="entry name" value="CREG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13883">
        <property type="entry name" value="CREG_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045277263" description="CREG-like beta-barrel domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="CREG-like beta-barrel" evidence="3">
        <location>
            <begin position="46"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="229" mass="25061" checksum="DEB8DC41E7619A5F" modified="2025-10-08" version="1">MATTPARPCMLSMLLLLLSHVNGGILGRINTVRTGGASEDVVFPRPPYEQRAIMARWLVHETSWGVLSTLDRYTGEAVGGVVSHSDGARSSPSGRLYFYVTPMDELTQNILAHPRCSYTISEQQRRGSGADNPALGDGAGQPCGGLDPEDPACARASLLGRLEPVTEEDLQEAQVAMFSRHPRMADWPVDHHFEFFELRVEEVHLLDWYGGMAIISGEDYYAAAVDDAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2E7</accession>
    <name>A0ABQ5S2E7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006889</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48447">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48447:SF1">
        <property type="entry name" value="REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045630702" description="Reverse transcriptase domain-containing protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="703"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="839" mass="93206" checksum="6C1D99FF6B98D954" modified="2025-10-08" version="1">MEAGVRQGCPLAPLLYLFVAQALFSWLNSQEIGLRVLPSDEDRVTACQFADDAEVALEGEESVPAFLEAMDTFARASGQRLNLDKMEILPIGAVDDHHQGSTINGLKVVTTATALNLPMDNQLQPPQPDWPEMLALAYTRMQRLAHYPLSAFGRAAAVSNYCLQTVTWHMEHGGPPPDTTLTYIERLAARLVDRKQGPEDQGQRFTGVPSRLLVGHPSTGGFGLLPLRQHIRARQAMWAVRFIRGALMARRERERVMLLDTAMNTVAEEEEEPVTSGNVRSLHPWIRTLATYLEITHPAFKPWALTTAQATGPWLGGETLPEEISRIVGALGHLPPLEDVSNPPLAPGDWCWAAALWGNPLLPNNSDNGGSINGNNHRPGLETRHPLMASCRKLLTVGDLVRVYDMLEHDQIPLPQYMTWLDWTQQFLDPSASPTQLRDKESTRMALSALCNDISPAWIAAARKVVNRLQPQQPDRVEDRMQQQMPAPPDRTTVMAMIVRRLGWRLPGDSGIVPLYNLTVSQATQLQGVSTAEERLELLSAFVQEAQGMPTDGPAPPCPVTNLQRFQATLDRLWTAVRWENKNKETLWRLVVDGIPIPGNTHLSRVPIKPCGCGSYGADTDQPCSPRLHHFWECPIAKAVREQIDRHLPLPTSATIEGGLTRQQLWLVQAPPGCEQAPWDVIALAALSSMEYGRHCLRAATREHRPQWPSTHRRRQQQQTHTAEQGPRQRAITDFFNHSGGFPQEEEEDANTHPIISTTQPAMDTPTQTATSPLDRAQARTVAHFWARLHSFAALGIPHYGWDQVGPQHPILAIVGTQMRCADPVDITNHGAARTEQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2F6</accession>
    <name>A0ABQ5S2F6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-ribosylation factor-like protein 2-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_006428</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ARL2BP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1520.10">
        <property type="entry name" value="ADP-ribosylation factor-like 2-binding protein, domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038849">
        <property type="entry name" value="ARL2BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023379">
        <property type="entry name" value="BART_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042541">
        <property type="entry name" value="BART_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15487">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15487:SF4">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11527">
        <property type="entry name" value="ARL2_Bind_BART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BART" evidence="14">
        <location>
            <begin position="46"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="178"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF11527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI63456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="203" mass="23130" checksum="2CBD33F5A64D787F" modified="2025-10-08" version="1">MPPKGNDEMHDDDDVDLCDGEILDGDEEDIDIVDDGSNLSAESRRFDCIVGALEDVIMDPEFEELREGFCRENCQHFEDSDENKLIYTDIFNKYTELVESTIDKKLKQAVPGFSMQEFMSLLESHKDHLMSEVFDLLLSLADFEAFKEVMLSYKRDASEGPTFEINCTPLRVYVEEQEDGDARPDLDRTLQISPIGAGPKAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2R4</accession>
    <name>A0ABQ5S2R4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006974</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="419"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="516"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="658"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="715"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="777"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="946"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="302"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="777"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="791"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="953"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="964"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1011"/>
            <end position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1029" mass="106576" checksum="027259B6FE522456" modified="2025-10-08" version="1">MPKTKLAGLKQQLRAAEEDVQLKAQQLEELREQVEEARSKIEHLEGEVNTNAALEDDVQTLLRHQRETAAAFMDYENRVLALELQGLVDRWRTMDIEELAQQNHDLREECLRLQQENMQLSKTVAENSLSARRDLARIESALELTVKEKMREMHDRLRQELYEEMDDKTKKMVAEHGYLQDYVRDQAAQAATLDRHCRMLQAERTRCKVDSDLATSLSEQQASHAVVLRRQLKQAYQTVEALRRRLMETEARAQAAWDEAVRASSRAAVAAAAAARAGLRGGDAAAAAAAMAEGDVDSAYGMAGEGAEMAALESQLRHAKQRFERMRLQRDRWRDRSILYEKACAQLQAALQTLQDQARLTLPSGVKQDLDAIWCAAKGAEAAVDSGAASSRPSTPYLYSSMATPQSGVGGVGAVTETDSRAGQWARSSWSSPSGVGGRWPAGGGAAAAEAARGRVASARVSGSAGQTRRSLPSRPRTAAPTPHDGSGGGASAALRAAEAIRAAVVLNPPLPLPVSPSPSPLPASPAQPEEVNRIREGGGDGIGPSLESPADGAEPTRGSSPGRVAIMSRLYSNEAEPFKLVPKVQYDQTGWPVVPELRPLSPNRMNGGTGTAQVGSRTANLALQPLVLPQHQQEHLAVPVVPFSPLAAQPTLGALAGGAARRTPGSMRPHSAVEPSPAVTTPRWTICKSLTGLTSPAAAAAAIDAAAGGGDAMAGSLTPDVRQDGSSQQPPPPSLMLQRPQALSTGPEPLLPNPVGTRADGYRRTVSYAAEADRQTWQYQRSRSQSPEYAAIARATSATSAEAGEQHGGSKTNGGGSGSGSTAAAADSALLTAALSPAVPGTGGVPEVGLSGGALYTVVHNIAVSAGGSAATSSPSTAPTVGSAYLHPTTSSGPQAAARALQAFTATSAAGATAIGTAPGPVFLSNPSALLAALRRPPSCMKRLHNPDVMFSGTRSSSARGGAMWGQRGGSSGPNGGGSVSGGSVSGGNVSGADGGGGMGWSNAASNSSSGGGGGGGGSSIGESATTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2Y6</accession>
    <name>A0ABQ5S2Y6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="344" mass="36603" checksum="2D0691D86D9E5FFC" modified="2025-10-08" version="1">MMMLSNGIEFRRATAADAAHLNTLRITIIVLLMKSSFGEYVGDLMTEAALVAAAAVQPPPLVWRPPKSPPPSPSPSRIPAPPSPQPPSPPQSPISLYPSPPPPRPNKSPPPSPSPSRPSSSPPKPRPPPPPSPPPPKIGVSSPPPPPPPPPPPLPSLIPSGMLPPLQRFPPPPPPPPTPPKPPRPPPFPPRPPVQPPVPPRPPAPPPSPRPPRPPPNPPRMPPSPSPPSPRPPRPPPRRSPPSPPPQHLHPTPQPSSPSPKPPKRSSKPSRPPQNPTRPPRKPLHLPRMPPSTPPKPPTPPFKPPRPSHKPPSRSPKPPRPPPKPPTPPPKPPRRYSKPPSPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3G5</accession>
    <name>A0ABQ5S3G5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_007157</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across the thylakoid membrane. Involved in delta pH-dependent protein transport required for chloroplast development, especially thylakoid membrane formation. TATC and TATB mediate precursor recognition, whereas TATA facilitates translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00236">
        <property type="entry name" value="TatA_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011429">
        <property type="entry name" value="PRK14857.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33162">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33162:SF1">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI64003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="114" mass="12038" checksum="DC39B317CE198AF7" modified="2025-10-08" version="1">MLARRIQTSTIGACSKPQRRMLGAPISARVIVPRSRQVTCQGLFGLGLPEVAVIAGVAALIFGPSKLPELGKSLGKTVKSFQTAANEFSEELKAGMADDTKKPAAEEPKKTDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3T2</accession>
    <name>A0ABQ5S3T2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007877</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="450"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="353"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="536"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="460"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="474"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="640" mass="67448" checksum="041B08C48220E0A9" modified="2025-10-08" version="1">MQQHRQEYRRQTIPPKGLTCGQTSPSPATTPRQAPQASPTTTDGLGVGAQLHSKSDSPQATKSNPDSGDVLESGRAPVHPSSTWIWRCSGTGNGTFARCNGPSSSVATSGGGLGRAASTDSPTVHRRRTGIPPLLSPVSSRTAADTKRGARVTDDGNSSSDDFTARLKDACARAQRVIERNADLLALADGLRRFTSGTPNSGCSAAVAATAVAADPAATGQTDPQLLLGQLERESRTAPVAKVPGDDVDQSRGCSSQGVKGNDDSGAYVLHVLQPLSGEQLASPPSRGCSSARKARMVEMGSALGSEDGAEHENSSSSMSGASSRFVVGSRSVGFNGGEAGGSGSGASDGGLATGLAEQLERERARADDLEARLRLAEERHAKEIKVLQSVRVQEVLMQNGRLPSQPPLSPQQQEAITGNGMESAAMAAIFTVAHQSSSGFRTPVGLFSPSSQRTPGSMTAPAAVTPGAATAALQRSLSSPTKSRTCRDGGDGGSGGGTAAVAAPPPEAYMSLLSPVRPSQPPTPSWPTGVMPGWLEQHLKQHLQKQQQLEAQLEQQRQRERELVAQMEQQQFEYETEVVLRERRYRQDLRQQRSAASALEQRNWQLQSEVRKLRDRLDVRDEVIAVLTMDCEAASKTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4J4</accession>
    <name>A0ABQ5S4J4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="301" mass="32863" checksum="F020CB0480736FE8" modified="2025-10-08" version="1">MSGGNTPLGAVHPGSIPRQIPAQGAPGGPDIVGMLTQMQQDLASVNARLNSQGATSGPEFDLRALLEDYASPQVQRSPSSARFLFPDFLLHNLPGTMSADNLVLHNYRGIPEWQHLEQAQRGHPDRTGSLVYEMSILLTLLSVVELVRSALSIIRRFIGHCNLRQDWLVALSCACDTLLYDVAWMYNIISARINCLAEFSHRGRDGLVTLHQRYFTQSERPLLDDVTWEFDAEYAKDRAKVTLRSLAEQEVHRSSNSGQFRGHGGLGRRGSGRGVGGRVFQPARAIAAAGALAQTGSGQGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S550</accession>
    <name>A0ABQ5S550_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LisH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008397</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI65025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055289">
        <property type="entry name" value="OFD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39063:SF1">
        <property type="entry name" value="OFD1 CENTRIOLE AND CENTRIOLAR SATELLITE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39063">
        <property type="entry name" value="ORAL-FACIAL-DIGITAL SYNDROME 1 PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16045">
        <property type="entry name" value="LisH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="584"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="638"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="737"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="820"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="914"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1133"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1227"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="221"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="480"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="829"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="951"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1036"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1065"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1099"/>
            <end position="1112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1335" mass="146775" checksum="2743CF1EBBB48249" modified="2025-10-08" version="1">MSERADAREGQLDQAEFKKKMLSRLQTTGVVGQVKSQLRAQLLSQLQKGQIVHLGPPPGDKPGLKRHAVNSMIADYLAAVQYHYSLSVFKEESGVESRPLLTEDEVMDVLKVDRDTSFYQSYMKSKAHAGSDSCVILNLLSAISEALAAKGKESFTQTIGGDRYQMEVRMMQLENEYQSRLREARMAPGAGLEERLALYRQEIEEQAAVEVARQVERIREMEVAAARLDEASKARRAMEAERLELDRLHSERLAKLRQREEDMMDKLRRQQRDVENVAYEHRQRILREEERLRNQKIEIQQQQDSRQEQLRQLERSLELRERAVAAREAAAEKKLAEATEAVAEAQVEARQDVEREYLELKNGLAQQRAQLEMDRARMQELRSEAIADLAAARAKDDRLRAVETARAEAEARAAAHAADAELCRLQAEKLSLELDQVREELSRRVVQAAEGAEAMLQNAAGAMNMLNAGAMVSRNTGSQIRDAMAQLEGAKEEAASASAQVTELQQERASLQVTLAALGTEAEALRTQLRRVEALLDEAVAARATALSSLEEGQFRMFQQERELSEVRNALLRAKEELASLRLQAAMRRPPTRGSDSPGRRSITVSPTRRQPPGGAASTISALSLGIALGGGPGGPLAAAGVHGPLQHPHILSQGHQHNHQHRQSGQYQLSASGQLSAVEAPAMSNVEFPIAAAPRDPILERLERLRRQEDIMASQEDAFRKRMAVLQEARNRNVAVAGSGGSAGASPNAPGSPMPHEAFFPQQHVPPAGELFPGASDESPLPYMTYVMHSRPSPGTQSKPQTQFVLSADDARDQELADKEEQEMLAYQQQQQRDQHIYRQQQQQQLQHYFSRPTPFQHQHHQQALLQEQEQQHQRHVQQVRYPPAQPQKQELQEHQYQDIKPYPYDNLNVQAVQQQQQIPRQHTTNDGRGPVPAGTEVADAPTTSESSEHISQSSSLSQPQQPQQHPPYVSARSTSHGAAAPPQQQHHHQQQEQPLQGASYIPHSNGTGDGTAPRTGMPAGPGQHGGAPSAEPAPAQPNLPPPGLVMTSNLRAFPPLADEPPEAVEPPPPPPAKPVIPGLVMVSGFTPRGSATAPAPAEEHSEEHSEELHLPSRPTMNVEDVLQAKMRMLQEQRERALAAQQQQQQHPPPLQRRTSRTSQSSHISGRGSVSGEHYFVTSGGIGGVSMEASSSGAGTGAKMPSPRTEDSTSEIVAGEAYDTVAPELSSWHRRGQRSHEGSGHLSRQSSVSRRELVRGVSITRSETSVLSVEVPGLSGSSMRALSIMQTLDDIPSSLEGYELPGLNDDDDDAMEAPGGDEERLGAFAAEFSAGSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S588</accession>
    <name>A0ABQ5S588_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_008471</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF758">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="427"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="427"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="443"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI65050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="540" mass="57694" checksum="2C6A6178D4A300DC" modified="2025-10-08" version="1" fragment="single">RQFSKPCMSLRHSSACPLKAAGSGPCVCCALERQLREMLVSADGAALSPDEVVSVLHRISPAFKPGRQQDAQEFWVKLLEMLGQEASLCALLRSSAAVDVATHQAIRVAAAAAATCEPKQDLGSVECTERIFTGMCQNGRRCTKCQQVTSLGEPEEFKTLMLSIDMANSVAEALDNHMNQGSFDTKHYCTMCGSHEEATPSTSLLSVPDVLVLALKRFESCTDMGARCGRSKMNKHVEFSTTVNLEPFMLPGLSGPGEATYNLAGIMVHTGDLEGGHYVAYIRGNGGSWWCKDDKRAVKVPLEEVLAQKAYMLFYEREQAMPGQANMKTTITAITTAKRDGMYDAAAATTTSLTTTSTTTSSLYVTAHDDCFDLSTSTTSSSAISDAADAATVHGYWDPAAAAAGATSSSSLHAATLATAATTVTTTATTTPADDPTVDTAASRRRRCPHRRQQRGRIAATPLPPRPAHAVVAQPARKRLGSSLLHSAEEELDGSVQQALKKPRLGTGMGFAPLKRFIGDYVSSVGATLHQWTGVLVQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5C4</accession>
    <name>A0ABQ5S5C4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007793</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="172" mass="19152" checksum="9E04348087F0EAE4" modified="2025-10-08" version="1" fragment="single">VAGTRNLGLQFRREGKEIIDGYCDSDWAGDTNSRRSTTGYVFRVNGTAISWSSQLQRTVAASSVEAEYQALSSAVREVLWLNKLCGDLKADSDGIRINIDSQGALDLGNNPITSSRSKHIDVQHHMIRERVIEGDVALRHCRTEEMVADALTKPLPEGKFVWCRREMGLDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5L0</accession>
    <name>A0ABQ5S5L0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinesin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="38470" checksum="3D3E5E76D27D1C73" modified="2025-10-08" version="1">MRWLRAQYRKCKTSSWAPTGPATWATILIPPRPLPIVRQVGATEQLFRQNVIGSREVLSPEDPWVMLAINNLASVLKAQGKYCSILGYLRVVSKIGPPMVIFRSGLCRPTQQRYNKYGEAEQLHHHALEDFERVLGPHHPGTLAAVNNLALVLHDQGQYSEAEKLHRRVLESYERRHGSGSPSIYAATATADDNTATATATASAETAIFDNDATAAHDSQGMLRTLNNLALLLQDQGKYGEAEKLHRRVVACLDRLLGPEHPDTLSSVTNLASVMKAVGRYDEAEQLHRRALLGVERVLSSLHSDTLRSANNLANALKEQSKFDDAEVLHAIVASSLEKVLGPDHPDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5W5</accession>
    <name>A0ABQ5S5W5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="2144"/>
            <end position="2225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="815"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="874"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1032"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1279"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1432"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1513"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1739"/>
            <end position="2116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2285"/>
            <end position="2332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="593"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="656"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="671"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="876"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="887"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1032"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1294"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1542"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1615"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1649"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1668"/>
            <end position="1681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1746"/>
            <end position="1771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1774"/>
            <end position="1791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1799"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1858"/>
            <end position="1885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1899"/>
            <end position="1910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1911"/>
            <end position="1925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1944"/>
            <end position="1973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2024"/>
            <end position="2039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2054"/>
            <end position="2072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2073"/>
            <end position="2082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2288"/>
            <end position="2303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2407" mass="250634" checksum="E4B678B54DB8E0AB" modified="2025-10-08" version="1">MAFLAATQLADDFLLATQPDLAVETDSDPEENVILPPQQVGVLVVDTEQRIGVEEYPLFLGCNIIGRDITDIPGADEARQERLRQWRSENLEVHVEAIFVPEPSLSKTHAHIYLADGGLNCFVTALCTTNPSFMVPAGAASRAHTMSPGVRYNVRPDDVLRFGRLSCRVLCGPDWLARGQQQPLQQSSLPQQQQQQRLIRLQPWQGNGEAEYQQQGLDGRLGSEHHRGSPQHVQHRMDQGYSWQRQEQQHQGFISIWPSSPGAVKRPSGAAMAHGKAGDMEGLQAGRAEERLGGVVSGGAGIGPTAGSFLPAASADAVEGHTHGDAGATGQPGQPRFVPPTQDVLVGLLPQLPEGQQHEAGMLAPRLQQHQHGPLPVSVMPVIALVPPQGAGAAGGLSRPDTLTAAAGAVGPGPSGAVHGAVQPSGAVLVQLYGGARALLPGDAAAAMLPLQVQMPFPLAQVVSETEEEEEDGEGEFEAEEKGKDAAAVAPRAGTTAPPAPPAPWWELADSLDDTHDGAWGASQSAGRPEEADTDTGSPGPGGGAEVLHLRAGEEAGNRSRQHPSRPLSVKLEPVDKCGELQSANGVQVSGVEHPDEDMRPHSVGDDDRSVCDDDAVMCDLTQPPDEEVLGVAPGDGFVTADDAGDEARVREQQENQHQLPCSSTELGPGRPRPVGPSPPQEAYSDDDTAPPSPPSPSPSLSDAANHVGASEDEETSRASEDKAGTGGGRIMASGGADVRSHLLVSTDLTRMVLAERVPQMAAAAAPVVPPTQLVETSLVPPPMYASVPPPPQQQHQLTCPSHSLQMDSHQGRFGQVVQPQCRKQQRRSSYPEGREWTDRSVWGVHGMAGEAGGGHELLPDAVGGCRRLLLGILTDPSPSSSPNPDSDGKVDGKPVSTDHHQHPEDLKPHFAPLRYPAAPLLQRLEDQHQLQPSLRPRTAAVVEALSKSPSPTVHGNDVGASKSLPAAAVAATIGPGIAATVGIPAVGTEPPCTGPLAPFNISQSASPWDLNAVQQRVGRLVSLLCSAESSEPSQEELLQPRQLQQPLKQMPSGRRDAGNVATLGGPGQEGPAPRADAVEMAPSGPSMPLSLVNEQPPPAQPQPLLRQQLPEQHPLLLNASAVAVPPTRAAGEMQSLTGAAANAELLPQPRPPLPSPIPPGSPSVSVRSFVMPGFGTQIAADALQQLMMAESAPDRNAESSPCPRLDAAAAELRLARDTVLTHSAAELDHRGNPGREMQVLGGAYRGGRVGGGGRGRIGGAKVEQFGYSNADYVYEFPADAGDDGDEDYVQTPRRGRGRGRGARGGRGRGGRGSKGARGAGKPPTGLPRSTGAKRRASGTPNEVSPTGGAGSARVPPPGKRSRHGSREASPVPQGADESHHQPQQQQLFGELSYDLQCRALQQQQQQQQQQQLQQQLLLQLQQQGLIGIPSAVSQPVPAKGPKGRGRGRPPASGRGCAALTMAAAADLAAAGVAIAPGGPHVAAERTPGSGTAAQIEAVVPKVGQLASPRLQWATRRRRTSASLSREGSAELLQGSGSSGQSPPPPPPHPSPHPQTLPVLSSQGPSPLLPTPSAPRPQDDPPLTLAPEVTGPLPLSPSLQRHQGEMEQLSPERQPASQHQTSPELVNLQQRHLQRHDQVSPTPSPLQPQPQQQQDVQEDPSTPTLDDEMQHAHHDGGDDGSHLVAFPIRRAEPRPRSSTGIGSPGGTRGEGALRDADGSVAVAEPCTFGVATVTGAAAAAPLQEDPSLRHWEEAQTARRRAGGEDAVKEPPHAPGTTSTPVGTTSATAATGRPFKRPRMVAASVTTAAAAAGTAAAMGPASEPPSVATVEGPETSAEHDKREPASATTHALPKPEPELQPQPQRLQELNPQQQQQLPGLGEAQQLHAEQPAQQMRDAVGGVGGSGSGGRGGGRRPAPKRAGKRKAAALEELPSTEQEPVLEPGVEVKPEPSAEGLAETREPRVRSPGPSRDRLGNGAAAASPIGGVILAGPAVEEQRTPHGAGEVRGTSAPEGRPPLARRPPSEQQLQLEQQKMQQQQQGCSSCSHGEAAGAAPKRRSSTGARKPRASKARHAAAPAEADGPTAEKPDVVVPIGHDSSAAASPQLPRHAGSSPGVATGLRRRRVSSHNGAAGGSQLAAAEGGLAAPTASTAVIVSISRNIPERERNGLAAAVQQLGGRVAEDEEADAGGFTHLLLARNAFTTSMKVLAALAAGRPLLDAAWLNACRAAGMWLEPHSEHTAVDPKAEKQHKFRIWDAYRTTQREGPMLQGRTFYATPLRSLEDAKKVPAGASTGSGSKASKAIGRTSTGGAGRCSGAATASPPPAECSPGSGAGPAQGLALIAQLAGARLLVEAREVVAGETVVVGQPGELRWARQHLPAGTRVYDKDGFVQALLHQRLDGIKPAFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S606</accession>
    <name>A0ABQ5S606_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="614" mass="64442" checksum="A140E96103498795" modified="2025-10-08" version="1">MGGHGSGGGDGVEAALGGAATGAPAAMGPHLPAAADPDTPVAATPAATVLAMAHEHTRPSDPGGGDARSLSGADAPSWDPWGGFWGAPPSEHPAWDHDGHRGRRFVPLGGGDDGMLTPATEYHPALGDGPGGSGPPTRSFRRGVRTEGGPEEVLPGRPVVASHPLYSPWEGDVGRRPQAAAPHREGVLSGRQGLGGASQMGAIPPPAEAADQRQPRAPALLEAPCWESMHFTTDAFAPPDPLPPPVLWSLAQVDYVAMAVKRQYSLRGILAHSLLSQIGETPDSGTLRCAPSSSDADPPVWTATHLMSFLPAVARFIGCPVQMVRWEELYASLEGRLQPRPPLYPAPAPPSAGPAAGTPAAPAASASYAPGTPPPDPPQPPHGPPGYPPGEPPAPLPPHSTPPPPIHPALGSPDECFALRATGVVDLVGEPQPRQPGFIVAPISPHWIPTKGARDYARAYDGASDGFTAFKSHALSYAECALYPLTHEKRKGADYSEVSDFLPVNTPLRSTYTPKELGRVVYRHYAHYAHCAMAHGGVHVDYRGQRAASGGQDLIAFWLKEVQARVLPNAQRLHKDFDQWAYPTGVEPNLAVQGFSNKFDQLDAQLYPQGPTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S650</accession>
    <name>A0ABQ5S650_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RegA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34491">
        <property type="entry name" value="A-TYPE INCLUSION PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34491:SF74">
        <property type="entry name" value="DUF4456 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="690"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="964"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1053"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1114"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1182"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1271"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1332"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1453"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="427"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="552"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1281"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1336"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1390"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1467"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1516"/>
            <end position="1536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1536" mass="163563" checksum="E6AC70A557922754" modified="2025-10-08" version="1">MQQQQPVLPPLSAHHTHAPESPSVDQGALPASARPPGNVRMSSPANGSTPLALHPIANWGAARNPDATPQTPPQVQLPNSWLDSSVHSSAFSVVPCGESREVPPIATSERTCPRTLRNISGGGSCGSLESDGTCGSGGSPGGVTTASGNPPLAALDEGPRRLKNTPQSEEVPPPPPHQPLEDRRVSAGGASAAAEDTVIQNHGQSDTEGAAGALDGGADAAPLATSNQQDAVQNLDEIVAALPPGPIHVTVAVRVGAGGGRRPRGGGARVAERDTRTGYMRGHACGVFDVPRYLSGRDCIHNGNKWMSRSNFEKVGGSKMAKWYRSILVLPDLEPLGEWLERHGMQVTKGPPRRSRKRAAESGDGDEQLGDGPDDDGAAGSQEEEPMQVDSETTLAIVANSPAATTGGQLRNGNHPADAPAWRSDGQQLQPQQLPLLNNPRNSSMPAAAAAVMSEGNEELPMRAGHWPQSPEATVAADSAPQPLPQPSHFPHQDVHPQQQLWTSPAPQQQQRQPSPPPLHQPLEDRRVSAGGASAAAEDTVIQNHGQSDTEGAAGASDGGAAAALPATSNQQDAVRNLDEIVAALPPGPIHVTVAVRVGAGGGRRPRGAARAAEEDTRSGYMRGHARGVFDVPRYLSGRDCIHNGNKWMSRSNFEKVGGSKMAKWYRSIRVLPDLEPLGEWLERHGVPVAKGPARRSRKRAAESGDGDEQLGDGPDDDGAAGSQEEEPMQVDTEANVAAVPNPPAAPAPAAALTPLASLPPLAPLPPQAAAEAAAAAAAAARSAAAKAAGSASVATGHASHPGVDRPVEIPLDRKFLQALLNTLSQEQLLSRNALESAVNATEEEHDHELYGPPGAYVDYSAYGGIRNQGTMRTGAGASAISRGAGAPGTAEAKLLAAAQAMFGAAMAAARHHRSGDLPPAELPTDMPRLRWRALHRSQSSSDPMSSSPTLHLDLQDLQNARDLEDGHENPNRGSRQALRQELQLQQQQQHQLQQQLQQHLQQQQELQLLRHIMAQQQLVVGEEDYYYAEEAERQIEVEEGAGRMGGMGLEVSRGRAEPPPPPASLPPLLRRTLSGVSMPPNTRMRRFGSSESDAVGMAEPEVPPAAFLVPNHAVGASSRGSSGPGTQPRLERPLPLQPLSLPPAPPSPSSLESAAALARGLAALQGLDVAAAAGLGPTGLGHEHLSRTGPSAPHAYPGRAYPGALRSYRHATESDQAAGSKHNHNHSIPNRDMYQEESSPRGMLLAPSGEGSRGGSANATAGALLMLQRRQSRQQRWMDEADGEDDAAMEEGDAEEGQGLWRQMGIRGEASHAAAQARFLARLQVELQSHPLPDQQQQQQQQQQQQQQQQQQQQQQQQQQKQRQRQPQQPQQGRLQAKGQQPFPQLDVHGPRDVEAYAVRERTARMCEPPPPQRRRRMGGYAAEDLEGVEGGEEAGEVMEYWLENFKRRRSGHSIGDLQSGNTPSLPLPLPRAQPPPRSSQTYYTHQRATVMPVSRDTAGQWRPPRPSAAPFEPQGRRVDWQLQRTRSEEEPLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6R2</accession>
    <name>A0ABQ5S6R2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribose-5-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_009147</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050262">
        <property type="entry name" value="Ribose-5P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43748">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE 3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43748:SF1">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE 4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI65575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="291" mass="30820" checksum="854E28D1939113D7" modified="2025-10-08" version="1">MLASRPPRCCFQAAARNKWHQRRMPIVQCAQKNAVAESKRAAAKACVDRFVSNGALLALGPGEMTNLVIEEVGRRLSAGNLQGVAGVPVCDVAAHEAAFHGVPLVPESRLAEATVVIADADQLDAKVNAALVGCACEPQQLDIPRFIAALTPGSSGGAPKLVLLTESSRVVGRLGGVLPVWLGADGWEEPAEELDDIFLGDAELWRRSSSGQPENPRGGDHPYISLQGHTIVDVRFYEGLKLYGEDEPYDKIAEEIQKISGVVAHGLLIGKAAAAVVARPGEEGGSHLVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7G0</accession>
    <name>A0ABQ5S7G0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF46">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="102"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="602" mass="65092" checksum="FC024304E2506073" modified="2025-10-08" version="1">MPLSRLHRVPCLQNTSCVGRTAAICCSPLRVHLHGGASLDRLSVQLSASACSAAVKSSRRRVVPMAVAAGDGFTADANGDSSVVTPDAAEGRRVVIVGGGWAGFGAAKHLSEQGYDVTLLEASPNPGGLSSGWRTASGRAVEAGMKGFWYQYKNTFKLLEELQLPSWPLTDFTTSGFWSPRGLVTEAPVFSKKPRLPTLVGQFVYTFPLYWALPLEDRLTMLPFLATLADYVSSEERFEQYDKMSAYELFRQCGVSTRCYNEFLRPTLLVGLFAPPEDLSAAAVLETLYFYALAHQNDFDVCWPRGSTAELIFQPLVEKIRTAGGRVLGNRLVTGLTLDSATGEVSSVEAVDRSTGEIASYPTDAVVFAVGISGMQKLVSANPALAKQPDFRSIMELRSLDVIATRIWFDRRVATRYPANVLSGFETTAGATFFNLNDLQDEYQNEPGTVISADFYHANSLLPLSDQEIVDRVVSHVATCEPGFNGAKVTDSVVLRFPKAVTHFSPGSYQHRPFQATTIPNVFMAGDWVKGVPHGANGLSQERAYVTGLSAANLVISRLGGNGQPAQILDVEPDEPHIAAARAAVRGLREVVAAAGLRSPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8B8</accession>
    <name>A0ABQ5S8B8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_009735</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01801">
        <property type="entry name" value="Ubl_TECR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF28">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI66029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="304" mass="35223" checksum="3BE543A4B7AE0B71" modified="2025-10-08" version="1">MKLEVVSRSGQTIATLDVNPEAPVSELKRKFYGIKRKYYPSRQRFTLPLKPGEKRGTVLDDSKSLSHYGLGDGGKVEFKDLGPQIGYSTVFFWEYFGPLVVYPLFFFFPKYLYPHLEAPKQHHLVQKLAVAYWSFHYAKRIVETFTVHKFGHATMPIFNLFKNCAYYWGYAAYVSYFVNHPKYTPPNEKASLACLGLALLMQLGNLRSHIILSNLRKPGEKDYKIPRGFLFNNVTCANYTFEIWGWLLYSAAVQSIPAYLFAITGALQMTQWAIAKHKRLVKTFDGRDGRPKYPRRWIIFPPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8F2</accession>
    <name>A0ABQ5S8F2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbohydrate binding module family 25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009399</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005085">
        <property type="entry name" value="CBM25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050583">
        <property type="entry name" value="Mycobacterial_A85_antigen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48098">
        <property type="entry name" value="ENTEROCHELIN ESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48098:SF6">
        <property type="entry name" value="FERRI-BACILLIBACTIN ESTERASE BESA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16760">
        <property type="entry name" value="CBM53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01066">
        <property type="entry name" value="CBM_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbohydrate binding module family 25" evidence="2">
        <location>
            <begin position="661"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1067" mass="115447" checksum="4335902765938BA4" modified="2025-10-08" version="1">MSALSAHMPLKPCSSRCHSPHPPRLLSLGNGVRGPPPIRVAMPASAAAGTSANGAVHQPQLVDDPSSATHITGVRMVLDLPPTTLLRQLPENHQAVSEHVAIAVANNVVEATGVHATLVPGTSETGSALDMGTASSAATITAAADAKLEAAEEKEAVSTEPFSLSHSSPLAGGRLDVVHWREGRMLRIWTPSGFVQSESPRSAYPVMYLNDGQNLFGDRPTLSGQSWHAAEAAAGLINSGALPPFVIVGIDHSGSIRSYDYLPYPPGNANGYRLDAESWPGGGVDAYLRAVVDEILPYTERTYGVSSAPALRGFGGSSFGGICALCAALRHPGVFGSLLVESPSLWFAEERLLKHDLPDFTGPWPTRVFLAMGTKEYSGIRRTKESEFDAKLVSYANEVAALLESKDLVRGQGLQVVIEEGATHTEAAWAGRLAPALGFLGLHWWRHVMSHRREQLYFTFPKKLRAGQPAVLFFNQARSVVLAGRPRDSIAMVYGFNVDWSVGGGRLEMVPAVGLTALAKEITGAAPSQSAVALEQEPAGAAAAVPPLSSSSSSSLPLPTNDITDDFDAASVSQWLQASFTVPPEAYEMHMAFTDAAGGIWDNNAGANFYARVKMDMTAEQRSHTVEQAMAEGPAWGDVQSIAHKRAGCKYFTTPEVLMAGEPAALYVNRRQVHGLEHNPNLRVHFGFNNWQSGCQEIAMKPTSLWRGENIDWWSTTMTVPSDATELSFVLTDSHGIWDNNRGNNYVCPVKRLRGPDGAAKRVEPRGVESVESMPHAAGTLHILTLTKRQGSSAATRASKWTEEKRVRVWTPPGFNTERPPPGGYPVLVMQDGQNMFEDWLAHQGVSWRVGYTASDLVTRGELPPFVVVAVDSVGAMRSLNYLPYPPGTGAGGFRGDAERWPGGGLEAYMTRLVEEIMPLIQQRFAVSSDPAKVAFGGGSFAGVAALYAAMHYPHVFGCVLAESPSLWISEGRFLQDMRMHRGMLPERLFLGCGTREYSATRDHDRDDVDALLLSYCQEAASILESQGMRGGRLQFLVEDGAGHHELAWQWRLTGAMRFLFNAWWDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8L5</accession>
    <name>A0ABQ5S8L5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000029">
        <property type="protein sequence ID" value="GLI66270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="453" mass="48740" checksum="B68E73E0CC598792" modified="2025-10-08" version="1" fragment="single">LVDCAQESADEHGEVQQREAAAAELRATVVELEKRHEAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRRETAEAGAAELRAAVMEGEKRREAAEATIVQLRAMVVKMEEQVRCSNEQITKCNSLQERFANVQKSMKLYRFQSRRLKRLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S960</accession>
    <name>A0ABQ5S960_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_009641</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF69">
        <property type="entry name" value="FERRIC REDUCTION OXIDASE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="461"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="772"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="814"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="8">
        <location>
            <begin position="483"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="995"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="217"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1023"/>
            <end position="1039"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI65959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1094" mass="119961" checksum="F973D24380E4B790" modified="2025-10-08" version="1">MQSYTPSSAMMHRVLRFAVVTAMWVLVVTGLSTFGFFYIMTPTRWFYVNATDFLSWRSSLVLQYTFVNATTGKVTPLLPNTYSFILIWQWCATLAAALGAAGLLWEYSSANKRAQPASSARYGMHAQFRRRRSPSCITWISKGAVRIRRLLQWQVPPRGLWRKVLGPDGLSVLDLILLLLWFGLHIMWLREMTMKTLDARRAPAAVKVVSTTVKSNSSATLKSNTTATRATNSSSALPSPSPPSFNSSSGVNLNKVTAAPARLRSQLEVTNTTGTTPTIISSNRTLNSTKSNYTTTITHVTTKNSTSTSTATKTTTLKPLPREVQNSMAKYTGMVGNFDLLMLFFPLPRCNFLHWLLQSDFPRMVKYHRWLGHGTLMMYSLHGIIYMALWTKDGTLSANMQWSMNSYVNNIAGLISLIAGWILWVTSIPYIRRRFFNLFYASHIGGTVVFMLFAFMHRKDIAPWVMPGIFLYLLDVVLRTLQQAFNSTSITASASVGPEAPAAAFLSPHGNILTLTIQCDKSLSWSGLDIVFLNVPEISWWQWHPFTLATSSVAGGPETKMVLHIKTYNHWTQRLVARLATNTDSLKLYVSGPYHGAHRKWITNFDRHIFVAGGIGVTPVLGMLQDLIARRRAMAANSDLKPAGRVSLIWLSRSRDELATMPYDVLQEASKTGADAWLDLQLYLTSPDQQQQEEEDGRDPVIVQENAAQGGERSRRNSNVLLQTAVSIPLGGRLGVDGVRPSGDSLGTTTTSSKSCTAARPLAHPYMLNPQLWAVAVVLCFAGGFAGFICSQAYDAHISRTVAVRNDYAHVGMLQFAALGLGATLPPALLMLAAHMLLRIKSWRRCTADAEAAGSEQSKHCPSTLIDMKDAAASCAACRVTSILHARTSVNSTVTHLSEDNAMDNSNFASFCDAAAPCGVTGVRVSCNGSKHVPTQGHICAEPYIKHGRPDLAVLLAAITRTGSTAAATAAVTWAHPQPAPNPNTLVAEPQQLSRLDNQNPTPGTPRDPIPRGNDVAGDQSLPQPPLAPLPPLPPPPPQDAYTDQDPSDLRVAVFVAGPRLLVETVEERCARLNGVWGRKGRCYLELHPLTHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAC0</accession>
    <name>A0ABQ5SAC0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027948">
        <property type="entry name" value="DUF4436"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37330">
        <property type="entry name" value="CONSERVED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37330:SF1">
        <property type="entry name" value="CONSERVED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14494">
        <property type="entry name" value="DUF4436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="397"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="542" mass="59775" checksum="DE08069486D3B5E7" modified="2025-10-08" version="1">MAATVRTRASSLSSALSSCLQRRTNNSRGGEASHTDIEAAGSGGSSSHSAERCTQPLKALGIFASKACGYAANGMAFILEPNLPKGWIPLVHLLVVVMLVLLLPWGIVYGVTMNKFTRNGPIAVVCGLREANIDLITPDNVGDYDYFPFINIPEAPPLPSPSMINNEPTAVGQHKLPPQAPPPPPRPPSPPRAVRQQLCEQDFEDAFDYSLSYPANLPTYPNNTVFVAIVIQDVDVQRFVLSGLVRASFQTPPVQSRNTDPSGDDSSARGIITIPPINRKLSLVINVNGNDVLRVSGSVSSSMASLDNLPVFAERHTRMYPFDTYRAHIRLAISLQDDDHGGSTRVPFVAAVMQKGSGFKYHVRKPRDMYLMGLDGRNDFSEASFLIWGNRSAIARFVSIFIVLLMWILSIIIFTQVLYIVWYRKFDKVIEMASFTTTVLFALPQLRSTQPGIPTEANLVIDMTGFIWNMALVSIACIIYLYIFYVTLVGEKENDKKQTESSQQTEEQPSRGQKPQTEEQPQEQPPTEHQQGHATGTIKNIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAD8</accession>
    <name>A0ABQ5SAD8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010883</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000045">
        <property type="protein sequence ID" value="GLI66887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051412">
        <property type="entry name" value="Formin_Homology_Diaphanous_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691:SF6">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="186"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="341" mass="35996" checksum="4AFB77BE9335EF3F" modified="2025-10-08" version="1" fragment="single">PPPPPPSPPPPPPPSPSPPPPLPPSPPPPPPPSPSPPPPPPPFSIQPALPPRCSVCISARLQPPPLDIQPYRYDNITCASIQQSISKSLNTALNNSFIPMVSYFAGNASLCSSLEAKVCGTFFSSYDAQDFKTTVEGLLPFLIDVAAGGTVCRAELNGYQVAVTTDGSSCLPVASSASCFLPFTPFPNCTCNTTQGILPFAVSPRYVTGLKTATTTEYCFTISTIPDNLVVPSACAVANDVLTKVEWYANQNMSSWVYGINLYPSTGPKVTRSASWGAAGTNSLKATPINWTVAQASGSRVCVEMKNPRTMSDLCLGINSQCYASTFNSNKDCCPIFRTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAI6</accession>
    <name>A0ABQ5SAI6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010266</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="3">
        <location>
            <begin position="64"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="128" mass="14888" checksum="1B9A5B7D32E9E7CD" modified="2025-10-08" version="1" fragment="single">PVVRMVGNVAVELALPTDMTIHPTFHVSLVRPYRSMEPVPEPANAPAAIEPVPETWIAGKQKFYDVERVLDYRARRVGRHRRKRTVHEYLVKWTGYTDEHNSWEPARNFSQDMKPLLEEARKRATQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAI9</accession>
    <name>A0ABQ5SAI9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010492</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="252" mass="27048" checksum="4BEAB879D6C26345" modified="2025-10-08" version="1" fragment="single">SSALCFQRLVSTDEFLGPYLPPEQCSLEALNDVGQKGHSAAVLADAEPLREYFKDWAGSLLESKEEVARQARSWTKAMAASFLQPYGLGDTDTSTVTCCGTKGGVETTVSERACDVGRGRVLMANLDGLPFPIISQAIMLTTEKVKCFPLEWTPLYSGCPVRASETTPKLGAGWVEALGLNADLVGKPEAVDGCPGAVRVRVRPAGPASLGLDQQQRGAGGWHSIRLLCHLPGVTSEGALRHRLLRNQDRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAR5</accession>
    <name>A0ABQ5SAR5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000039">
        <property type="protein sequence ID" value="GLI66716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="373"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="653" mass="70718" checksum="EDC6D07891F0100B" modified="2025-10-08" version="1">MSQMRIQQSCARNPLFQQDAGDVLRALQHTGVAENPDVITQSMSNAEANGQYAGQSTEYALTPFLDSLRLTQGDPMYRAISAQMDNLHQSKASANSVLDQPGASDQIESTWVCYGRYDSNLHWQKLHLQGLAGRLRSTNNRQSPDSSFTVCRVHLTFTRGSLLHSLYPQVFKQLHEHDQLEIRNLLMALESQKQQLLQLAAENDSVRQQLLTRGTELAAATGAQVPRDEVMVASVAIQAGSSHTDTAASGRQRPLVRKQAGMGLNQPDEPSRDSLDAAAEGGRTGSGPGSQASPVVMQRGSRPAAVSRAVLQPPGIGGVRGSGPPTWRNSGGTVSRHSRRPYFILFLPYMLHPAHHCWRFYAAWQYSWTARRTLWLAVAWVLMFLLAVGLLVGLLVPWDRLGRTKFTLDPEVVAVGPSHVSLVVQLNRAAVVYYALLPEASTAGAKTSGRRLASSTKLNPGAAISGKELDVGGRRFDLGRRSGCWRSSMPCLMVHGASGHRRTLATAGAVQLDDVEGDDVRDITGGSAGPRAKLFPFVVACGVVDVDSKYTNYTLTIRNSVLTLDRGDVYGGSVKPNTSVDECTSSFGMVLNQTTPSEFVGPFYARRCQRCPVLKPGTPYVVLLMGDGGRRTGVAQVRFTTSTASLPPAPMPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB60</accession>
    <name>A0ABQ5SB60_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol polyphosphate 5-phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045849">
        <property type="entry name" value="IP5P_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048869">
        <property type="entry name" value="OCRL-1_2_ASH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666:SF22">
        <property type="entry name" value="TYPE I INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666">
        <property type="entry name" value="TYPE IV INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21310">
        <property type="entry name" value="OCRL-like_ASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="4">
        <location>
            <begin position="92"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="539"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="589"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="774"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="551"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="783"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1106" mass="113442" checksum="53545725C476E547" modified="2025-10-08" version="1">MKKLLVNIGGKVAALADDIASYNDRSRYVATAKPPGPGPGAVPCGSSASSGRGEEAGAVLDPVLEHLLRNVRSRHVRQHFARTPAAEYTQQRAVTIQVATFNVAGKKPPPGLKLDDWLGAVPAAGGMKGRWQPGAMAGSMTAAGASGGDGPDILAVGFAEIVPLNAGNVMGVWGSAHVDAWDRCLAVYLNGEEWATQRYGPSAPAAAGQAAAATAAATLLETKWQGGGGGAGATPGSLSGSSAAVIGTGSPGSTGGGASPNPYRAVSACGGSSSGGGSSVRADEEYVQVASKQLVGVYLSLWVRRKLLPEVRGVQVTTVATGFGGYLGNKGAVAARMRVYDSSLVLVASHLTAGEAEGDELRRNADVHDILRRAVFTNAPDGAGAVAMTAASAALISASLGPGHWPSSTSGISDHDLVIWVGDLNYRISTVPTAATASPAVGNISGEGRPMGQQGLTDADVRTAIRAGRLDLLLAADQLNRERAAGRVFQGWHEGRLTFLPTYKFKMGTHVYNGDDLPASVSAGSLAVAAATPRVAPLAAPQGGEVDDGDSGAAAGPGAAAADPEKYKRRTPAWCDRILWWTRTRLRNHQTHPPHPSQPHIAGGHGGSGTATAAVGSMIAPGLQQLAYWRGELTVSDHKPVAAAFVAEMVSYDRRRIEGVLEAGMRAVDLMQQSLRPRVTVEPVVLEAGEWVALGRPVPMRLQLINTGTVEAIFHFVPPPHPGRREQGSKFGLDDETPPLPPWLVAVPAEGVVAPGEACEITLTVLVEGGAGGAAAQLSDEQPSSSSSSSPPLTHGSLDSILILRVEDSGDKFVSIGGRYQRSFLGMSLEALSRLGSRPALPPLGAIELAEHLGLGEMKQQLEGASVAAADSGVDDGTVDPPKELRTLVSELLANSAAALRVPGILVDSTALVLARAGHDDVTAAEAAVQASPAAATGGDEEAAVAALQPTNRAAQQALLRQLEPLRLILDAGGQIPPGTDPHDVAAMLLLWCGQLPYPLIPAVAADAAAAAPPASPAEAAALLRRLCSPLQLVVVSELLRLWRAALEPVAADLNGLTAARLAAAATAWWMPPLAAGAAVGAGSGRRKLLTMLMAGGDASISWPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB93</accession>
    <name>A0ABQ5SB93_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_010963</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000051">
        <property type="protein sequence ID" value="GLI66924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="15"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="12">
        <location>
            <begin position="182"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="137"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI66924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="277" mass="30806" checksum="9C6D489051D78247" modified="2025-10-08" version="1">MPPQTSFGLQDPHYFLGRGEILSWINDTLSLRLNKVEETCNGAVACQLLDVLHPGVVPITKVDFNANNEYQMINNYKVLQDCFNKLKIDKYVEVARLVKGRPLDNMEFMQWFKAYWDQVTMGQPLAPYDGPGRRALCKTGDMKGSSAGGSKGAAGGIKAAPTPAPRAPPVAAPRPSVSSKTASPGDRDQMDKLNHQLEDLRLKADGFEKEKEFYYSKLRDVELLCQTPVIADMPIMKRVQEILYAATSEDGARIVRETQVEFAGREYSEEELSGQDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBF5</accession>
    <name>A0ABQ5SBF5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_010615</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="165"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="314"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI66682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="748" mass="82516" checksum="23BFB8FCC6351141" modified="2025-10-08" version="1">MMAVRLKRLSLRQGLIFILCIPNICIFLLGIRQQWPEKMKWSGGPKCIGWKATFACIPLGPREPSSDKTCAAEVREGAGYCLCEDNITVARVSCGAHRFRCQEKCAQLAVDPSVAQVLPKPLTCGPYDGYNMARLVPAWAAYRAEHWVEARGERQRRVLSGDDRAAAAAADDDGGAIVDSTVSSSSSSQSSNPSNNRTRISTTSTTSPMYHRGSGATWPWGELGSDADTLSKISSLIWDIVDKVMGRNGLGDARIPDNRIDKNDWVREGRRMRTRDVEKLKASVAGLLRDLPPYPNAAATAAAAAAAGTAAGATDARNADGSSSISGSTSTSSRSSSPPAAPGFSGRGVVMVGGGLHYLTPAWTNLHVLRRTGCRLPVELWFPVDEFPTPAVEEALLGLGVMSRRLAARDLAQRGFGVKVAALLLSSFQEVLFLDSDNALLRDPTYLFESEAYVSTGALLWPDYWDRTAAPEVARILNISASLLPAGSFESGQMVIDKARHWRGIVLAAYMNVYGAVFSELLSCYIGKGDKETFAYGMLAAGETMWVSPVKPGSVGLVTVVCSPRGRGSKCREQFLGNTMVQYGTDGLPLFFHANYHKWDLALPDSFDKGWNRRWQVLQPNRRSVAEVIYGGSEAGGAGYDVERFVYDTLRAFRCASWFDSYHADRLRMNETPAASLDGFHPLPDYITFRDFYRYGWTGNFEDLAHPPFSLGDRLVSWYYGKPRWWFRRLNRNLCRLRKRVLPRWLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBJ2</accession>
    <name>A0ABQ5SBJ2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053283">
        <property type="entry name" value="TUNICAMYCIN_INDUCED_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34454:SF2">
        <property type="entry name" value="PROTEIN TUNICAMYCIN INDUCED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34454">
        <property type="entry name" value="TUNICAMYCIN INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="583" mass="62305" checksum="763F2C9432E36441" modified="2025-10-08" version="1">MSSWTSRRYLGRIMMTRITYQTICFYPKPQNGISKIISAPFALVQLLLAGFLIVAYVPVSNANTLSVVHTVNDLLNSLVARAASETIAVVRKNSKVELELQTVPMETLQVFVGRQHHLGLEVDGVTYEVLLGNEQEAMEEARRRSTAAAAAAAAAAAAAAAAAAAAAGAEADAATAPQALPAAALTELQSSDAPADALLLAMAGSKEGALLQLFSGPSRSRTLELPETTLAGPLELVLPAPEPVSIYSPLAADVGGPVRRIVIEAGVTVRVAGLRRVSLRRPLDLPRLPGQYLAEVYAQSATGQAEEGFEASPGILYISEKIRKAAEMAANSSSSSSKKRGGGKQSGPSQAVSLEVETVAPGAFTITSAPELPPPYSRAAAPRLKIRRLAESTVELSLRQGQLTPADTFNPAALLRQWAWPLPHIESSSWLTYETLLRSVLASHPFNIDSVIRRGIGLRLTKSAIQSVHMIAFDMEVHARAPRPAELEGSPYQWNENPLEIWEAVVHVQRKTQSQAKGQAQMAEDGGAAWEDFRFVPLHVKRKHSFQNTLTVSRSAMIMALTGGLNVTAGIDGYGTDMYPHAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBN4</accession>
    <name>A0ABQ5SBN4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010826</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000044">
        <property type="protein sequence ID" value="GLI66844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027887">
        <property type="entry name" value="DUF4464"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33588">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33588:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14713">
        <property type="entry name" value="DUF4464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="237" mass="27615" checksum="F52564F47EDC5E97" modified="2026-01-28" version="1">MDDDLEIKGPGYDPIADEFATYEDYLDSQINSTDVYYLEDVELARQLVELGIRGNGEVIRREEFEQRKEASEQARQARLNKKPKKLASQGKHVEEYPLLQALKDREEAVRNGKLTTIIFIRDKNSKGQEVSGYIDYGHRLKTESMEPYFDRKKRLMPRPSDLSFYNWETQMSTSNPTPNFQVIADNEAGLLFKNKRDRKVINVDPRAKPGDNSTRTELETTEYLQVVVYDHVTRRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC94</accession>
    <name>A0ABQ5SC94_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FGFR1 oncogene partner (FOP) N-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_010710</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CEP43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018993">
        <property type="entry name" value="FOP_dimerisation-dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER/LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431:SF4">
        <property type="entry name" value="PROTEIN TONNEAU 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09398">
        <property type="entry name" value="FOP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGFR1 oncogene partner (FOP) N-terminal dimerisation" evidence="8">
        <location>
            <begin position="54"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="118" mass="13225" checksum="403D80DD3187C880" modified="2025-10-08" version="1" fragment="single">MATLDDLKTALRENLERNGKLRKLKAQMRADVFAALNTSTEADQKAMPQLSNENLIINELIREYLIYNQYRGTVSVFVPESGQPAVRPLDRTFLASHLNLPEGPNSAQLPLLYSLVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCB0</accession>
    <name>A0ABQ5SCB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="24857" checksum="CC1889C16AACDF56" modified="2025-10-08" version="1" fragment="single">MGMLFQPACVAASSSNQSQSQNTATTKVADPNSTATNRTWIRVSCGLRPALPSYYTAVQEWMQYAMQPSADADMTLVEVVPETAVETDPWVSASTRILGATEAAMQPLLTANTSWLQSLEAAVAAGKPLPSAYLTPRPGALGGPTMAPLLLFYRRDWWEAISGLTYDKARAAIPPTWPRLVALLEQLHQLDLDLDGDGKSEHVLCVDMMPACKGWSLLAAIYASMMQTHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCE1</accession>
    <name>A0ABQ5SCE1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MAT1 centre domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015877">
        <property type="entry name" value="MAT1_centre"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683:SF13">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06391">
        <property type="entry name" value="MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAT1 centre" evidence="1">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="181" mass="20168" checksum="E3A63AB839A8B6D0" modified="2025-10-08" version="1">MDIDLDKELKVRRRILAIYNKDREDFQSKEEFDDYLEEVEDIIWRLSNNVEIERTEAQIRKYRQQNQEQINAKSARQANMDVQAVLTQLTAASLAAAAAQAAKAVAPPMSGGLPTILPKLTQDYGTMLDGGRNFKYDRRVLDRVGPEAEAAGGWTAGVARARLAQEAFGSLLSSVRIVVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCI4</accession>
    <name>A0ABQ5SCI4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011432</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039726">
        <property type="entry name" value="Prp40-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864">
        <property type="entry name" value="PRE-MRNA-PROCESSING PROTEIN PRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864:SF0">
        <property type="entry name" value="PRP40 PRE-MRNA PROCESSING FACTOR 40 HOMOLOG A (YEAST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01846">
        <property type="entry name" value="FF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51676">
        <property type="entry name" value="FF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="362"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="408"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="540"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="607"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="674"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="746"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="491"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="647"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="731"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="948"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="584"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="799"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="299"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="367"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="497"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="517"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="647"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="985"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1017"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1045"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1056"/>
            <end position="1070"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1070" mass="114461" checksum="4A1A1F5D9C23E815" modified="2025-10-08" version="1">MAGRGRAATLPAWMTSGGTTVAAPGAPSPSPVPAPSSGPAAPGVTAFGAPPGALAHQGPAPGGGPMPGPMPGAAGGGAGALPHLAPPGSGPPGPGRPIAGPPGAEAPAPGTHPHAPLGQAPAGSAVSPGPVSPPPGTQPPQPLAQHPPAAGGLSAPPGGLPPPGMMGPQLHGAPPPGMMGPRPPLQGPMGGPPPPGAFHGMGPPPPGMGYPPHHLSAPPGMIPSPYGLPPGAAPMGMPPPGWQPGMGPPPGYPPRPLPGFPPGMPPPGMPPPGMPGMPMPTGPMPGGLQPLPGAPHPGAPHAQQQQPHGGPLAAQMPGQPPQQQQQQQQQQQQQQQQLAAAAQGAGTAATPGAAAASGGGSTGAPKEWTEHVAPDGRKYYYNTKTKQSSWEKPEDLMSSQEKAEKVAAAEASGWKEYVAPDGRKYYYNKTTKESKWTVPDELKAAQAAAAAAGGAAAAQAAAATTTAGAAAGKAPPIQVVSLATAAIDSTTARGVDAASATPAATAGGVGATGLASPSATDGPSQSAASGDPKNYSFATKEEAKDAFKELLASVGCRSDWTWEQAMRLTVNDPRYSALKTLGERKQTFNEYVQARRNEEKEEERRRQRQAREDFLAMLAGSEELKVTHPFRRAKELFEDDPRWKAVPEREREELFHDSQRERDKREKEERRQERKRRSNAFRDLLESSGIKPGAEWRKVSKRLEGEEEYEALDKVERLEVFQEFVKELERREKEEKEKEKEERKRQERRARDSFKELLRRHRDEGLISVRSRWKEYSENVVSEESYRAVEANTSGSRPKELFEDLLEEMEQEYDKAKDTLKQILKDGSFVVSPTTSYEQFEDALVAAASAAADSDVAGKLAALLPTYKRTFFEEALAKAKVREAEEAERRRRARDKLSSYMRHCRPAVRGDSVWEEWLREHGREPEVKALPLEEVHVMFDEVVAKMRRRAEAERDGDEDSDGGGRSGRRRGSRSDDEEDGGGGRSSKKARKEKKSSKRHDRSDDDEEHKAKKHKKDKDKERDRDRERDRERDRERDRDRDRDRSGGGGGGGGGRGGDSDRDSGEIRDDGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCR6</accession>
    <name>A0ABQ5SCR6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="133" mass="15069" checksum="C8453B12D56D37D5" modified="2025-10-08" version="1">MMVLATNAARIHQKEEIQAARRRQAKSPQPSKARNSSRNATNSSKAAAKKVTNDGDVRPSTKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRRKKELFEEEVSKDERSEDEQDDATSAREDDDNDDSEIEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD51</accession>
    <name>A0ABQ5SD51_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_011306</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="12"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="338"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="382"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="436" mass="45371" checksum="ED6D54DF0F33F60B" modified="2025-10-08" version="1" fragment="single">MDDYEELNGDEDGDEDEEVVQILGGNQFTPEEIAAAEQRFEEAYGKSLLPSGPGGGGGGGGSGDGSGGVGPDQGGPAPVYVLPLYAMLPQAQQAKVFAPHPPGHRLIIVATNVAETSLTIPGIRYVVDAGRSKQKLLEEASSGQMARYDVRWISKASASQRAGRAGRTCPGHAYRLYSSAHFNDTFPEHSPPEIVNTSLEGVVLVMKSMGVDKVHNFPFPTPPDAAALRAAHRCLEALCALTPESGRLTEIGRAMAAFPISPRHARMLLEVLKWHKRASGGGGGDAKYDTDGVLSVPEQVARRAGRALPYAVALAAAVNIESPFVHIDTVGTPDSAVDGSDAAAAAHGNGGGDDVTGGKRRKEQTARGGAVRKRRGNGNGDSDGDDSDNPLEAEDESTEGERLGGGVGPGPGSVAAAAAREAARRRRQYAAAMQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDD0</accession>
    <name>A0ABQ5SDD0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ACB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_011700</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ACBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24119">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24119:SF0">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACB" evidence="6">
        <location>
            <begin position="18"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="141"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="207"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="255" mass="26871" checksum="7620990E63D73A5C" modified="2025-10-08" version="1">MTDISMNDDSLEGVATDDEELFGSAASFLTAAVISKDPRFDDKLKLRFYGLYKQATSGKCAGGRPGFWDVAGRAKWDAWAQLGDLPQPEAMNEYVELLRKVVPDWGQEAAGGSQPKAKGSMGPVFSCLAAGEEESGDAQAAGLKTLHEVAGEGDVAAVASMLDAGEDINERDESACTALHFAADRGSVDVARLLISAGADLNAQDTDGQTPLHYAAVTEHREVYDILVEAGADVNMKDSQGTTAMENAPPAWGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDE0</accession>
    <name>A0ABQ5SDE0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Prefoldin subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011409</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23163">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027235">
        <property type="entry name" value="PFD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13303">
        <property type="entry name" value="PREFOLDIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15824" checksum="77438FED967CA2DC" modified="2025-10-08" version="1">MSAGPREQELLSEFQNKRERIQLTWSKITELAAEATEHKLVLEALEKVDKDRKCFRLVGDVLVERTVGETMPAVAKNRGNLEATINTLKLQLDAQKKDLNDFQTKHKIRIRTENEIDAEEVAKAKEGPKSAAAQGVLVSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDU7</accession>
    <name>A0ABQ5SDU7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="643" mass="68499" checksum="6063576C9C18A604" modified="2025-10-08" version="1">MSKGIPISQRPDLKDACLSTRLDAVFGSLGPPAAAGGWSLRQDIQPFKAGGGIEEYNYSSDEEDPNEGLKPLMPSQLVDSDDENGCDASRDPMPQLERADPDADAEEAGLGREQSEARAARERERMRATLSSRRAFEAEAEEDEYDRVATGTMDLRNRLRDAKPPGFTEVPLDSAWERMHGRWQQQGEEQQQEMDIRMPDTGLNGVSDTAAAMAPTEMDSSPVSAGDGGKGSGAAASRKRGRSGAAAAALWVPVEQQHLQAEQQHRVQLQQSGYADVEMMQATTAGGSTDPDAPGDVVVVDYDGGPRIASDTRTSRSGISSLASGAGSAIIHEPSGPATAPPVPSPQPPRQRGGRRVQWADFASGRSSDVDSTPAGNGFPNGHVNGSNGHTLPANGGSGRGGGDDGGARGRGHGGGQIRLDWQRRGFVPDHVRNPHKYIMYTLDEELVVGGGDRSTNNRNGRNGRNGGMLGGGGGGNGRLALGEDREGENEMRQGLAQARQAVEAASLVEHDPERVETTFGSGIAFRPRAARQQELQPRPRPQEEDQPSRQHGDSGERGPPDGVISGVLGISRGVFVMEGDEVEGPGNGEDAVASRMNVMRSCTRRGGGQRHFRARPRQTDEDDWGRDGGLGDDEMQQDERCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEG0</accession>
    <name>A0ABQ5SEG0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052061">
        <property type="entry name" value="PTE-AB_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260:SF1">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="1">
        <location>
            <begin position="172"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="287" mass="31364" checksum="9B6D542E593C695F" modified="2025-10-08" version="1">MFNKNFSFTLRALYLPIPHARLPHQQQQPQQLQGRAQLCVAAAQAHVGPVLQVLAKTGARWGGSAVLFAGAAYALAQSNRASESGGTSDLSSDVIPWVRQLAETNGVEEVLSPGQQLRKHPVGKLVVEQDHLFETMVASGQIKELRCFYDTRRRIFYSVAELGKEVCGFPQTVHGGLTAALVDETAGGLAVALWRAGDLGFRPPAYTARLEVDYKRKIPNGQIILISTEVEQLADRKVWLRAKVTNGKGTVYATGRALFVAPRWLPDWLRTLTGRRDAVATPAAART</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEL2</accession>
    <name>A0ABQ5SEL2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidinol-phosphate transaminase</fullName>
            <ecNumber evidence="3">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_012760</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF2">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="80"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI68357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="407" mass="45373" checksum="92C568EE6B70D414" modified="2025-10-08" version="1">MQASAHRGSWVRPAAQRQARARQSHLLVPLATSTMEASTSAPMVASSSQFLRKHMLKLAAYTPIEPFEVLSARYGRKPEEIIKLDANENPYGPPPEVRQALGSMPFPHIYPDPETRALRQALSKMHNIPIEHLLVGCGADELIDLLMRCVLEPGDKIVDCPPTFTMYVFDADVNDARVVTVPRLEGFRLDVEGIRRAVAEHKPKVVFLTSPNNPDGSMVSESDLLAILDLPVLVVLDEAYIEFSSEPSRMHWVTNRHNLVVLRTFSKSAALAGLRVGYGAFPLEMIEYLWRAKQPYNVSVAAEVAACAALTNMKYLDTVRNALVSERERLYTRLQEISFLEPYPSHANFILAKVTGGKDAKAVKDTLASQHGIMVRHYAKKELSGYIRVSVGRPEHTDKLIEALNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF21</accession>
    <name>A0ABQ5SF21_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="329" mass="34487" checksum="15CCC39C08A7D7A8" modified="2025-10-08" version="1">MDAGTPPLPVTPAYFASCCISGGAIAPAPIANADLAQAVETDASISDASGCFPSSSFITSFVGNTPFPTVAPKSGQPVGGHVKQKYRLVSPLFWLIHPVAAFSSEGGLLCTDSVHALALARAAAGRRRMMATLEEARRRFSHLSRSKLPNSWPSTAPSISTAAQELQFQRQGSSREGATGSRCLDEGSEMQRPMVAPPARLLPNLPLAPRDVGILSVGYAASIAVVPCEASKSVSKVPETQQGRAGEYGRGNQEGQYEMGGRDGGDSDGRQCANAATISYLQSYRILGTAKLSCESGCACEATTLNAYDPRPVSVAIPSDIKADLKLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFS9</accession>
    <name>A0ABQ5SFS9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA polymerase II subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013000</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase epsilon subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016266">
        <property type="entry name" value="POLE2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="8">
        <location>
            <begin position="284"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="583"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="597"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="634"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="709"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="722"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="759"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="807"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="836"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="846" mass="89535" checksum="DFC35DEBE20AFB1C" modified="2025-10-08" version="1" fragment="single">MSFALKKQIKKELLDSDWAGKVEEPAVTALANILQDQYGGQLHALFDILRSCNSDLSADGKITVASVQASTTRNGGVQQVELVEFTDAFSVPRLAFDPVQRKLYLNSKTRHIHASAESKHQVYLQRLLLIQQRLQRSRMFQQNHLLLPDSINRAPSVQLTDLQSLMGVFGVTRYVLGCISRSEDGRYVLEDTTGAVPLDLTAAETAAGFFTENCVVIAEGAMAHDGAFHVRALGLPPTEPRSALPLAAQRLNIWGGAGGGVGPDSEAGAMLALEEAQRGPMDRIVVLANVWLDRQEVLDSLHTLMAGFSGVPTVPAMFVLMGNFHSRAGGGGDAMLGGCDVDYGIMRELFGQLAGLIDQYPRLRAESRFVFVPGPDDPGPGAILPQPPLPRCLTGELRRVLPNAVFTSNPVRLRYATQRIVLFRHDLQRRLRRRSLLPLAGAVDGTTATAAAAATTSGSPSAAPAAAAGPSPTALWGHLSLTLLQQGHLAPLPLLGQPVYWQYDSAMSLYPLPDAVILADATAGQEDFVHDGCHVINPGCLPGGFFAAYLPCMQQAEMSELPRPGQGGDGDGDEMDGEVAAGAAGAEGALNRKGVLGDDDEEEGEEVETEAVARAAPRPPQPVSGERGPRAALQQHQPQQLRTNAAPKPTVITAAGKAKKPRQPRAAPLKSGAGGRGRGRVAAPPPQRPLSTCGVRGTEDPEVIYGADGPTGGTETAGGEGVEMAEGVEMQAAAAPEAPEGRGGGRSRGREHSRGRGNGAAAAAIARTGAAKDQGTLAQAWAKQPPLRPRPGPAAAVEEGTGLRDADLEDEEGRAIEDVEMEEDDQDEMEGQQQQARKRRAVIIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHI4</accession>
    <name>A0ABQ5SHI4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA mismatch repair protein MutS core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF20">
        <property type="entry name" value="MUTS PROTEIN HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein MutS core" evidence="2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="165" mass="18562" checksum="6CB90DD9D6DF4822" modified="2025-10-08" version="1" fragment="single">GKRLLRIWFLRPLVNLDVIRDRQDTLELLLRNSGVLIPAFRGELKKLPDMQSLLQRLALAQQRPDLKALQQLQSCLQQLLRLRAAFEQQLVPELAVATAAMREGEGQMAGGGETDAREFRPAACEWSTADPRVIAKPRPHCQYPNARSALGLRTYQQQGAEPRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHZ4</accession>
    <name>A0ABQ5SHZ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013575</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF6">
        <property type="entry name" value="OS04G0405100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="27766" checksum="59A90CEEB21E8F40" modified="2025-10-08" version="1" fragment="single">RMKMGLPFAVVLPGLGEVKSFDELGKKYQDWLGAQDVALQVLFTGLQSAMQGGVLGYMFGSISAIDPNQNGAVKPSNPALDSMVKAGPWGSARNLAALTGVQAAASLAIKKARNGKEDVYSAMGASFLSGVAYSVVSGSPNPIQSAVTTGAAFALFNGLIYQIGQAFKPDFADTEYDRGKYMLKTLGLTKYSDNLKKGLLTDNTIMLWNDSALAECRIPPGPRLLILHHLDTYRNPSNVLKPALPLPPLPPQPVTVVGTAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SID1</accession>
    <name>A0ABQ5SID1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF12">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="449" mass="46525" checksum="C5FDEFAE8F1E711E" modified="2025-10-08" version="1" fragment="single">PPPTHAQMYGSTNQPLQQATFAETLASWLGVGPIELPLLAPGAPLLPLRRLFLEVLDAGGSERACGGGCAATERQRTEGSHSGKRRGGAGGGVLSWGALAGKLQGDEKLGDLLKQVYDAYLLPLEKRLPRGTRPGRMEAWMQEDLRRRGALLAEQVVPTAEQVTLRLRASRAAIMLLQQQQQQQQQQQQPHQQQQQTQQQQQTQQQCGGPPTLVDPPAAAPAGASDDSIGRDAATAGGAMERDDEKGTAGAAVTEVLMKLVDAPHEEAANGAAVPMQSAVAQLDATVPLAREVMQVLRAAAVSAPTLLPGVVVTPISSKRARAANRRSGKGSKSGSKKRARGSVASAGPDGVAARGDSSAGTEGAAGMASDPAFGATTGHTPMYAEGAGGASGAGDRTTLPVPDLVNQKAAALLDVVASLRELRAGPPGCATQPPDLIANFTARWEMLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIF5</accession>
    <name>A0ABQ5SIF5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="362" mass="38525" checksum="B4426974E421370B" modified="2025-10-08" version="1" fragment="single">MKVACMMNSIDGASLMSCGTKLKFRNGALVSRRCLRLATFSAGPMYAFGTASNSSSSGGGGFVHRGINGNRKASLNVVCLSPRPIRYGGRSNTRSPSWSRGGISAGAAAPSSSERPLFPHIDIPSDPSISFAISTANTWQDLRDLYTVAARRIDPGQLLALTQRLAHVTGAVVWPEGVPLPEPTAAGGAPWAAPILIEQEAEEVVALAQTLCRATQVCLPEMYGMQQVAGLLQALVSLGVMPPPSWLKSAELVALRTQREGDMEGGRRATSELLNELLAMAASFQRVGHVPDPSFVGLLGAVQPLEEVQTDQADAPVLLVLAAPQLLCLARAVLLWGLQPPPEWPELFLQATYIRISRANKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIT9</accession>
    <name>A0ABQ5SIT9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014068</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SAAL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052464">
        <property type="entry name" value="Synovial_Prolif_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23424:SF23">
        <property type="entry name" value="PROTEIN SAAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23424">
        <property type="entry name" value="SERUM AMYLOID A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="740"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="816" mass="83832" checksum="3A3DFABD65C512C1" modified="2025-10-08" version="1">MALDTTGTSPGHIAPGNAHAAEADPAEQTAIEEASVIHLLRVLVSEAAPPAKASEDAMDHQSSTAGPAEDVNDITCEEAGCLLWDMSASRPVARVMLRNRALEALEIVIARELQIVQDADVSEAGLLPGVGDTVTPAAGWSSPEDRQAAQQQQQRRPNQNALRILEVCLGTLANLVVTQSSAAVEVSSRSEFLALLLTPRNPEDDNPNDIVPPPGGGALYVDDARVLSELFRLVSVALRCQGFHVWLRAFSSPAVLQRVMWVGAATADTQLFARFLDMLVALLHAASGSLATVETGSGRGATSGGDVVATVAADPNGAAQSGAGRACCRVLMECGLMGLLHQVLLQALRTCMPAEEKYSELGGADGEVFVSTLDSDRQTVASPPSVASGPAAGLLGRPTSAVAAAERGSDAIPAAAAEITPWSDGSSRFNQPGGVATSVPYARPEDAFHVVPSDGGGPSSPIHDSGTIAAAAMAAVTAAAAATGPVPLSDDAVDAALRLIEELVSPNGPLVAPGEGSESAWGRDGTGVAASGSSGAEVLLQLHAEKGLRPGLLQLLLGLLRYQYDSMQVVEGLLVVLVDLGSAVQGSVAASPPETAVAVAARLVELLQDSCFSPTDGTPSSIIQLQQGAWYLLAATLRGALLAVAAGADTVEPPSSAAAFKRTVRETELAGMSTQVRAEVPSATATMAVWRANWSSLERSLSALPALPRPDGCLGYSRACFVAGMGLLDELWGPQLEAAAAGEKGSGDGASGDEQGRQRDKRPRHSSCARTPTAGDTGSPLHRAGEEVSGFGNGESLMRLGAQLQRMLNSLEGHAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIY3</accession>
    <name>A0ABQ5SIY3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="145" mass="16070" checksum="D227227CCEC07DAD" modified="2025-10-08" version="1">MAVSAIPQSNWGMSTLQQSFRGQYKLARTPLVTANKRRVVTKAAAADDAAPSPSDDYHLTADTILWNAKHSAILRMRIAQVRAAEVVAESSSSRPELPPVNEITTNDVRQNHSSLSQWHEDQWQRLGATQHTLWDDELGHFGAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ52</accession>
    <name>A0ABQ5SJ52_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Eukaryotic translation initiation factor 3 subunit B</fullName>
            <shortName evidence="6 7">eIF3b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">eIF-3-eta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF3 p110</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014493</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="6">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the eIF-3 subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12278">
        <property type="entry name" value="RRM_eIF3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03001">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011400">
        <property type="entry name" value="EIF3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034363">
        <property type="entry name" value="eIF3B_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 EIF3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036424">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="62"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="716" mass="82664" checksum="9FCC69EDF7239E5E" modified="2025-10-08" version="1">MVQDLDPFAVARDLFPDMPPGFPFENFDLEELILPTGDDMGIKSDDDDIKEEDIQTQSGFGSCLVIQNLPKAPEEKYEKLMTVVRTIASKMGELRENGIYMPLDETTKMTKGFAFVELARREDVDAALLDERFANGYQLDKNHKLIFCRFDDFDKYAKVPDEYAPLEQVPYKQPENLHSWLADKLGRDQYVLRFGDNTVVCFNDGKRGRADLAFQREYWTESFVQWSPQGTMLATMHRQGMAVWGGPSFTRLNRFAHPSVRLIDFSPNEKYILSYTSHEPVTARDSYTILISIWDVRAGKLLRKFEGPVDEYAVGSSAGPNGALKWPVFKWAGGREDLYFARLGRSKISIFECPHMGLLGKESLKLDGVQDFEWSPAEPLLAAYSAEQGNQPARIVLIKIPERTEIRQKNMLNVSDVKLYWHPQGTYLAVKVEAWTKTKKSTTTSFQIFCVKDKDVPLEVFELKNNKERVLNFAWEPKGHRFAVVHGDGPRPSISFFTMRDDKGRLGVRELKMLDNRACNAISWSPQGRNIVLAGLKNFNGQLEFFNVDEMETMATAEHFMATDVEWDPTGRYVATVVSHVQQMENGVNVWSFGGRLLYSTQRDKLYQFSWRPRMPSLLPPEKEAEIVKNLKQYSKRYDEEDEQLIMQADADVLHERQRALDEWKSYQESQRNYAALQLDFKRELYGLRFHEKEFTIQKVMVEQIIDVKEEPYNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJL9</accession>
    <name>A0ABQ5SJL9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_014795</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Dispensable for SPC enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009542">
        <property type="entry name" value="Spc1/SPCS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13202">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 12 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13202:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06645">
        <property type="entry name" value="SPC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI70170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="125" mass="14754" checksum="A06F20BB27DDB67F" modified="2025-10-08" version="1" fragment="single">YNILDTKCTTITQTRYTDFTFIVPWIKHRVDRFCHLIFTFLCPILMDIEGQKRAEHLLFLIIVLAAAISFIWAYGNSDFRAMVYMNGVALLVALLLVIPNWPFFNRHPWQWLPPLDPNTETPKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJW9</accession>
    <name>A0ABQ5SJW9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoglycerate mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47580">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47580:SF1">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047361039" description="Phosphoglycerate mutase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="29376" checksum="A8A0864278C0D20C" modified="2025-10-08" version="1" fragment="single">AASPCGPSRRFVLLDGAFAAVAWLLVGSQPSHAALVRFPADQLNNRYFLVRAGESYNESVGEPLTNPVWKTSEKHGLSELGKQQVLLGLVPELERLGACGDGCWLWPSITQNAYQTAEVVAYQLGIGRSRIVPEYSFLDARGLGALETLRLEEAQHAVAAGDLLDADWRPPKGTDGTPHESSQDVVVRMRQALSITETQYFGEDVLFVSPDSDCLSVLQAAVLGVDLRNHRRFAFRPGEVRPLLLSTEAFDASPLSFSCPDPPRCTRAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK69</accession>
    <name>A0ABQ5SK69_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="44633" checksum="0881DA36649E0240" modified="2025-10-08" version="1">MSAEHDDTPHTSLASQPDFVVAARAPDRGLETVGARATQDPLFLQTPAHLIVLHSAVNTNMDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTDKTPGHSIADATRACVRDAKKYLQAVHGGDNDGIRCLFVGNTLEGAAKDWYRNWTNARGSYTFDELVAALLARFATEVQARDVEARNVLAAGSYHMRRDENVPAYQSRFEALVTPIADLTESERVFWFRRGLTKLLAKWCATDLVGAQIKSYDALVAFAKGAEIRLLAGEEEVKSAPRANAVQVQDPLGEAVALDQDLGATRPTPTAATRRSNGQRKARGGASSGVRGTGGAGPSSDPARNRKRGGAPSSAAAQRSDPNAKRTRTDDTFMPKWNCNRSEFERRKLANVCLRCAENHSTKDCPLMPPFVPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKI4</accession>
    <name>A0ABQ5SKI4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014718</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="8">
        <location>
            <begin position="739"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="16"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="504"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="637"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="942"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1140"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="951"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="964"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="986"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1032"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1046"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1199"/>
            <end position="1211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1266" mass="135481" checksum="1626051EC057C02A" modified="2025-10-08" version="1">MGGLFEFCFRSSGVRVAPDSSPRPSGLGLSLDHPGNDLGVKQPLAERRQVVDSGPLGPGLLCSMDSGGGSPGSGFDNGHYISSRSRQRRPSMEAGEALNTTLEMARQRRASSSYEHTSPSKSPKSPRLSWGWLKGKSATRLLGSSQDADKAAGCGLQRCRENSMNTAEQLQRRGFHVHYAKDIHKSVSTSTTKPIAVLGLEPSSDASSEGGAVPMKASPEGLTDVQTRRNHNKLFPLYVNGVAKTYFKIGASRDYAQVLRRLLRRDPLLTFALQKAIKNLSKGDCKPVSHVTSDPTGQSASLYGLRITPCVWSDTNGHKLPALLLEHNVPYESQDVMPRLLRDYAVLSHVPSILTLIDFNGHVLYQNAASIDYCGDLTSHNVETGDGMMGVLFQYEPQLLQEMLQEVNEGNYWQQVVRVPSSLKRHMVFEAETTWSYGSRGGGGKKLTAAVSKRFIQDALEPEFQLESFTTQHARSQRANTARNLMSMFQPQISLARQESLAPARCSGIAGPGPQRQDTRDSGVAISGPAALPVGRLGSITGPAPRLASNTLSPGTHVAFNLPNQLDDPAAGIGSGATTNAIIPTAATAGSFTAAASKPGMPPQHSHLTNTAQNRRFMTLMQSAERQQQPSFTASLQSFNTEGDKPESRGGPGQEECWHDISAIPLLDPVLDKQVILLLQTNVTARVELENRLADLTDAQLGMLEQLFPRHVIEYMLSHKHGYNSRNLTHLANHHDDVIVLFTDVVGFTSMSKEVEPSEVMHFLNELYTIFDDLVDLHDLYKLDTVGDCYIVVAGLTMQDDDGFTCTVQGDNDQRLHHAQVMMDFAKAILRESKQVLMPHDQCPVQIRIGIHLGSLASGLVGPKMPKFTLFGDTMNTASRMESTCKPGCVQVSEVFAKLLPFEDWQDTGGVEVKGKGHMQTFLWIPKPEDLIPRVAGSRLQHASGDGDCSVGSTEGATATGAPSSMATTAAPDDSATDRTEVNGTARANAASLVRARSARISAVRPKSSRPRSTRYARPTTARVRDGVVDASGGGASSGGVGNGSFSRPSTASSGLTRMSSTGRGSLGQSGTFNRSMSRRNVSFRFRGDFPYDEGSVEVEEDANPLMTILSAIRCSGTGEQEDGNSGGGVQVRRDITGGLRISSDGEGDDDKPERSGPPLGTKRASNGYVGGGARRSSSDAQGVHGSGGNRISFSGTQRVSASGARVSASGTRISANGGGSVPGHRTSVEGNTVQTIDATEALDELKACMVVAKADAPVKPGVKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKQ6</accession>
    <name>A0ABQ5SKQ6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015497</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="114" mass="12301" checksum="2A0CCD13321BAF8A" modified="2025-10-08" version="1" fragment="single">YHPSWVPYTTSWYRDRSSLGRLVKSSAEIVQAFSSLSVVTAQATLTAQRAQATQAALLQMDIDHDLPPADIPLDMETLPEELSAAIFAAATTVSTPQTLRSGTPAQSLRSIANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKR7</accession>
    <name>A0ABQ5SKR7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sfi1 spindle body domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1193"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1301"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1537"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1576"/>
            <end position="1600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1652"/>
            <end position="1720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1768"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1847"/>
            <end position="1873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1956"/>
            <end position="2002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2021"/>
            <end position="2040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2117"/>
            <end position="2176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1301"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1325"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1337"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1367"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1406"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1545"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1588"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1657"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1688"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1981"/>
            <end position="1990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2023"/>
            <end position="2032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2125"/>
            <end position="2141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2161"/>
            <end position="2176"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="2298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2298" mass="246283" checksum="E0C68F79D3B7C839" modified="2025-10-08" version="1" fragment="single">RDRPMLPNADVPRCVQRLTHGDWVVLEALQSSTVYAVRPAYEEAGVPALLYLSTLPPQEAVEPEACLEVVEQGEWLGLRSPFLEGLFLQARKKAPNLVFFSPRLGVWEQWKAEALYQGMLQQQSPAICLTSRQAPAITLSVRAWRVGSAPSPAPLRSLPCTPVQQLDPVSDHATHQLPPGSAYSLLGYATSVSSSLQDDALELSHHVVKGYVLRVLKWPLYLAFRLWRAEARTARNKRREAAAFAERRLQSAAWAAMQQWAAVERHARHMVMLACQCWTRHHTRRLVGALRQWHARAERSAMLERICALCRRRERRRRSVRVLETWVSYIGERRALRGRRELARLQLSKRQVRRVLQGWQEAAAAQRRHICAFHRLLQQRWVRCAIMRWHHLARHLAAARHAANAIFAAGAAAALRAVMVAWRGLVIRRRQLVIRAVLLEDAAARRLLGSVWCAWRVLVDEGAIRRQRVELVILVRQQQLTGRVLRRWLAQLRVAAVCRVAAEQLKRRRERSLLPAAFAAWCAAARLSTNGSRGNDGDARGTTTRLMGTVQRLLGYSRHAGLARRVLSAWRHLAAASLQLLATAMAVARMRRRTSTVVALSSWRKFTRACADLRRQLLMEHQAARLRKVVRAWGRAAADGRQLRIVTALVGRRLDFRRRYSLFRAWRGLVELRRELWHTTMRRHNRALMVRVLAGWRRRCATMQAGVAVLRRCRQGYEWQLLQTALHVMRSRVLLRHRIQRCKQKHAVLLIRGMLRAWRRWAARCARVSGFGRLLRSKWRQLVARGVLRAWFAASHSVALQALLLEVMQGAGGIWCAALCRWHNGAGSQAISPAVPVPARALALAAAALPHAESPQGAILRILMASHGGLSITGGRGGAAATAAVAEMLCMRLPTLVRLAMQPRRLAHAAFNTWRFVVARQVQLKAAAARHRARCRSLLLYACFKRWMGTVAGNRARRAAAMAMARRLVRSWAATCLAAWGEKARLFGRLRRRCFKLLALRMGRMARAVILAWRTAVREQRERLKDKLRNKLDVRLALLHRVVQAWQQAVATAAEMREAAEKRYTVAWMRFKRQVFVGWRHQTVFSLQHLVTCSRLLRTRAERRLLAAVTTAWSTYARLERQRRDDWPMMCRVFYAWRTLIHSGGRTAATAAIAAAELDIQGLRQVCLDAAALPSTSIAATAVASPQARSTVLSSAGQDGSESGASTAVDTGGLSSPGTVKPSFARCYSDAEERNGRAGALAAPNITSQPWYGMLQSGRSLAALPGGSILLPLLASMVTMAGADGGSDEANALREYLERLDGSVQRGERGDGAREESGAVQAQGPPSATITAAAAPVPLPAAPQPPHKARVMTASCQPEPWPQSQKGPSGSTSASRATTLSTSRSEELPIDGMATATPPPLPPPAAGSQGSSVTTAAGSAAPQPMTAANDFARSTAEPLQQVPLQLSKTPISRVPIASVPPEPGPMFQIRQGSSTTIGRASNKSLGLLEAATESTPTSSQPFDFGINNPTTAASAAAKAPAEASYHAYQGPSAQVTSAQHTLSILPPPPPPLQPPGLARSSERVLTASCAPEPVRISAGIQQNGGGRSSSSSLGRSSSGAISAVEPSGWAANIARPQAAATEAGASTRALAPTVTAPSVVPHTINIILQPYPHSHSRPVQSSSSPSGAARGVSPRGSGNGKDGDTPHSVASSGDTSSLASPGSLQAWSRRNTTDMVGTSEAASGISLRNAMFAPPSAGGEGSSCAVARQDGYLALSLPITERFIGQETPGLHDSCGPSLRGESPPDGTGQHYHDQALPLNPIFSPPNQLDVLISSSSGEASYADLMQLRPVMTSLELGPQSLSHGVMARCEKPPSTPSPSGCSGDGQLGDTSGIDLIAADGRAEGHQGMGNSIGDQNDASLGSLHFTWSGNGHAPAAATVSAAPTPRGFGNWAQVQVGRDDLAVLPGQVKSAAAAAARAAAQEDSRSVGQLNPQPQPQPTLPSQSSSWSSVGTDPAVPGAASTPELVHQGTVLQAGLHPAPSSIVQQPQHSSVVPAGQLHHGGAVPNGACTNPYLGTAVCRSAPAHRSHPPGPASSWASSSVDLAPATVFAPSTEASEHLSGPVLYSAYEWAHRRTTESSVTPLPAAQQGPQAGPSQTGSSTPLGRADGWVPGNSAAGRLESTSSGTTDAISDLNPSGTSISELEALLGALEAKSKSAISSVHCTSLQLAAAESGLATESTIAGRRLAKADTGGGATAATCGRDGDLVGLRLLDGIDALLGRATNLSAPGTPIFAARDGSDAPAMNEHSYAMAEPSYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKX6</accession>
    <name>A0ABQ5SKX6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015208</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045166229" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="57"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="259"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="540" mass="58931" checksum="C8D3F95B243898AB" modified="2025-10-08" version="1" fragment="single">MGRRILLSAASALLLLVGIATGAAQLDTADDIGLGSFGLRRLSAYYARNFGLQIPVACYQSSYFSPFRYYFRSLNLSRSDISTYVFQIDVSTCLVPKAECCRLWLDHIVVRTGSNATVRGVRLNGQAKEFDVGPEGVTIYNMSVEPTVQRSLTLELETPAQGYNYEADLCPQGRYPGSCDIISYDASETCCTERSALPYDMVFPKPPPPPVNMTETGSSPRPAATPPAPSPPSPPPPSSPPPSPPPPSPPPPPDGWDVCCVDDVPQSPFILTYQGSKQTGSDTAYLFQIGIRQVVAADYDVDGPQFGDCSQMNLRDVGIAIYGDLIVKSVLFNGSVVDYTLTPQAVVRNSSWLYIGIYRSLDTFKLNVLLDLIVTVRGQVPSLCPANDILQSSTACEWTMHGKDGLTQCCPHGITRRAGPFDDCCVDDLGKAPYRLDYLGADVSSNRTEQYFGVSVVPVTDVIADNVDETSCDHMTLDSAQIAIYKGVEVLQVKWEDRVMDFNITPATDLSKWLYVNNINAFINDFTEGLPLRLTVILQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLC8</accession>
    <name>A0ABQ5SLC8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="134" mass="14850" checksum="30C6A5825909B1C8" modified="2025-10-08" version="1" fragment="single">MQNSLVRRRDILISRKPNCSFGVNARRPQKAVLRRKLVVKASQQQSVYGNGSRIHDHTSGENPLRSLWSASLAAIAVVGKLLQAFVTFVVSCRDILPHFTVLSPQQALQRMILILAASLLMVVLLTTIDSGLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLG6</accession>
    <name>A0ABQ5SLG6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050690">
        <property type="entry name" value="JHDM1_Histone_Demethylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23123">
        <property type="entry name" value="PHD/F-BOX CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="526"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="604"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="689"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="526"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="630"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="767" mass="78661" checksum="C708AC4C964936D1" modified="2025-10-08" version="1" fragment="single">MNASGRHEDTNLGTGRSSGIWDGFRHLGTQLLAWPGYAAEPVEVVEMAGFTNDWVRTTGLQAPSLVKGCIKYTSWLQPLLTDMSDLSLLSKELGHDREVQTLDVAQQTPGPRWTMRQWCLYWKERQTLTPPTEPHHLVIGPSGASDAPRFRGSVAPAAAAAAGGGISSADDGANTEMDDEADSAFVHQGDVDQFRETSAPRKGIRDSNKRRLLALTCAPLADPGDGPAVGAAAALQVPPPLAAADLVAHLGLSREVMAAATGGGGSAVSVAASRHHVAVWPAGAATNFRLLPGGAASWWAVAHGRMVFLLVPPTEQSLALFAAWSASGARDHGVPFLDAWGSLSPPPFTTLLPLPLPTHCTSAAAQAVPPAPISPRAMQRSWAGGPVSDLLPGSTSGGTGSPPPSGLARVVRLEAGVGDVAVLPAGWPFAVVAVEDSVAVSGQMLSIYALGAALRCWELEDLLGVKQRARFPMFKHVMWHAAVSYAKALRSAAGLPMLKRQRTSTSRDRGGNAIGYPAMTVSHNAHGGSAGGARRFPAARPQDSAMSGGPVAVATTTPGGRNAGGGAHGVVAAGPGRAATAATSGADGRARALAAVVRKRTIGDQYADSPRRQSIAGDNAGPTTAAGQGATAGGAGTGGTLLPPRGTGVGRGSGKGASPSSPQYSGGGKRLRHITGAAVRMFNQLADDEDDVATATSPRERLQLGRPRGNSDAAAAGGLSGPDGSPMAAGSARRMAIGVRSGTARATEERPRRRVAQGRNWGYLLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLK0</accession>
    <name>A0ABQ5SLK0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="631" mass="64756" checksum="2ED7721BB9697237" modified="2025-10-08" version="1" fragment="single">PVGLNANLRGFRESETDGDYGEAADASYGISPTAKRTTTPGQTAPHLTSEQQALIFRCKPKPSRPSISDSDASLPAAASPTAMPPKCQEGLCSVYSVTRTSSCHLSDGMRTPGPELQQSPQLRQQLSIQQQQQQQSLTPQRQSLVLPRQASMLQQQHLMLQRQTQGSPHLIRQGQALIPSGALIHLVDSDGSSDTQYGCSKASAGSYDVRSLPSRSTTPTGRSVSIFHSRRDSQGWVGLHPATGMGSGGCGNTVFNATSGLATASAEASGATTVAGSSVDVGIAVSAPIVSTSFPAAYIVGDGSGSSLPGGSSIRSPLASPLSAPSLHGTQRLSICRSAFFQSPCVHAPVAPGSPQAPPAPVSPGTPSPPPQHSPSLNHHKQHATVISANVTTQGELASSYGRARSPSRRLLGPVGFSGRGAGAGGSGAPSGAVVAQILERSGGGSSPLPKLFSSFTSGQSFSGPIAPATGLWPQSTAASGGNEIGPEDGELIVTRRSCSAIAAVMGGGLPARGAPDRSESSGRTSTVLTNGNENDYECRQGGVVRAAAAPAGQGASADTHRDEGGRQSFNLGELLKEYEYEGPLSAAARQLAALHRAHEKSKAQRGLIKGQPSERQGPSSRWRSARTGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLS9</accession>
    <name>A0ABQ5SLS9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_015385</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF12">
        <property type="entry name" value="AT HOOK TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045198690" description="Protein kinase domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="937"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="1197"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="760"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="901"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1096"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="760"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="902"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="915"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1256" mass="135420" checksum="5C3E27E01BFDBAEF" modified="2025-10-08" version="1" fragment="single">MVTDSWSSWLVLLIIIRTIPSVICEVLPFIEVNETLVNLRSLLNTQGLLPGGFLSLRKNGTDILAIQQDRKLLPRDVVELTGKGSASLPAQYLESEWIYKGIWTSGRFSGNDSDPDAAYTVLDWRYVPIPALELQPGQSLTMRSMIWYAAYFLYPVSQALSDLSLDLILVPRNATLWLEDVVIMISCNDASRLLQSLCSSFDNWPYNPEVLVEGGVLGIRNLTSWAPWEGVRGAGGQIQWRNVSLTCPGHPGGGIPAPRPCVARPVNTAYELLTASRTALLEEKAKWVILSITSDIALLPEGGRWTPVPLNPEQHLILRGDPTARRTTLNLSGLADVWSLPGNRDMDTPPLDGAPMVLLYDLTLVNLPVYPTWPNGPMSLLAASLPCFGLSRGALWYGVEQIAVQRSAIVVPDMELHFLARIGNDQNFSDAWPGQPTGSFFVSFLERQSESDSSTGLLDQLHVQELKLMSEVRLTDCLLVSARSYQMWPDAAPLLTESTIWSPQLVAAQDPATVDKWGYGGLVVASGLQQALMDLAFCDSRPGYQPTRIILRHHDTTVPPLSSTPSPPINVLSTTGALKAADNCTVDGSSSDIGGGRVFSDLQGAVGRFSLVRPITLRNLVLYNLAPGGMVPTNVSVEDGNTTLSPSLQAIPIGDAAWKNSSLPLWFFKCARTAEELVYQPTAAELPCNSTSTPVRLPSRLVLENVTLVVPEREWRAMVAAILQMHAPDAMHAAQESGRRRQLDVFIEILDEGFEMPPFPPAIPFPPSPPPRPPPSPSPRPPSPPVSPPTPPSPPQPPPSPPYNEFTYEALMSFAAQSEVLSYNYSAGELVLAIAHHHGWSGTNVTITYKLPADAPGNATLLSYPDLVLPYSQLADMVIDFQANFTIRPLVKSMGPLLHDSSPLTDVPPSSTEGTQTSTSPISTSKTITASRRRHHYAWLAPLVASLSAAVGAALVIIPIVIIIRGGVMKTREESNGGAVGGSARCDDHVGRNNSLSTTVDERSSNKLEDLSVMQTVTREGPSVVEISCKSATVGFDAAASQHPPQQIPSGIQASLTSGQILKYNTTPGTTSSDAFSATKANRAALVEAFSKKLPNGPAPAVLDTEGGFDSGRGGGAVEGSAVTARDHMDMQAFSKELYAYYRALMASDPDAASPEILPEAEGTKTVQYGPVVKRNGPRNISDAIKNLQTTWKDKDLYLEHIIGCGASGAVYRGMWRGLPVAVKTLVVANDLEGKEGRARKQAVLEAAISLAMAHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM09</accession>
    <name>A0ABQ5SM09_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GP46-like surface antigen</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015648</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF58">
        <property type="entry name" value="RECEPTOR PROTEIN KINASE-LIKE PROTEIN ZAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045316111" description="GP46-like surface antigen" evidence="4">
        <location>
            <begin position="24"/>
            <end position="1656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="515"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="766"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1380"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1454"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1480"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1537"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1581"/>
            <end position="1603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="549"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="591"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="641"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="802"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1200"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1327"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1401"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="553"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="591"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="662"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="896"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="924"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="939"/>
            <end position="955"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1656" mass="171054" checksum="5AB6EB51A75A2CE9" modified="2025-10-08" version="1">MPRWLVKCAFNVILLSVGRLCAAQSPSPQLWSPPPPPPRPQQNIPLYYLDFMMQASLCSRVADIGCMGEDLQWLIDLEPFPLTTGTSGSPATDLDCIGSNDTGCKWRTIQDYRAASGPNTDAYFPLGLTIQAKNITRMFMLGHVIWGTNQYSVAISYIMRQLKTLTITCTQPIPVFAPYVLEPLIMALSGAVIDLGWNATSAAAAAAAAATSQGNSSGGSGSGSSTTPSNTTSDATTKPAPATFSRLYKPPTSNSSQLRSLIIQDCGLTGQLPPASFWLQLPALEHLDLSRNQLSGPLPATFGPSPPLSYLNLSYNRISGPLTASLARYGGCGRIMDLSYNDLRGNLPDEWLSSACTLRGKSQSGSEEWLRRRRLLMGEEHETGPDGGVAKVSSSPTLSYRAVKPLTAVTEGTTPSTASTSTSSDSYAFWLTYPMSYMYGGDDCPLLGIAWRKLVAARVMNGGTPFNFTVLLYGNSHLSTPGGTEASYATAAKEGLQFRVRDNACTIFEPLSGLLWVWCTFGGAAVAMLIVALVQLLFAVRLARMGMKPDRRGSKKRSGKKGPKARARVMPAEVGSGAATVPVSAIAPAPPAMAGSAVPTSGADGGGSGGGHRRVEGSTVAGREGVAATVENQFALQYGCEPGEGDAGADTGSSSVGRRQDGATTSPASAASDGGSGGGGMARLIIVAHARLYSANAARRLSSYGSAVINRFEAFCVWIVFDIPWAVISRAVVAITVHLSAAGLTFAMSQQQRTAYCGRLRQCGTYLYIVPPLLSAISIGLTVFLMKAMRQLARSMASRVAAVSPDPGPQNASGKSGGSDNVGGSVVKGDESASQFGVSGEEKLGRSKASSNDGEATEPRMTPPTPAKAPRLQERSSEQLWVSGLQPRRSGPSPPTSNESQQASSEAGRGTMPLFEACPLPVPASTSGMASASSRGSSKRVTPTASSQATAHTSIVSGGGSGPPVRPLLGLQSSGGGPVAESLAPVETDLGSFVSTAAGTAATGAAAVATGQAAGRREQIAGGLYGKPAEGAPGPLLPASPYGPLHQHAYCPDAAVPAVTRARASLESIFSLSRSGSRRGCVFSPGVVHDPEIRGCDSPGSGLEPLTALVAGAAARNLLGATRGPGSDDERARQCRVQYSLPIPVQPNEAAQPHGLVGTLTWHGLKSPSACGAVGEGIEANSSNPCSSASSYMEVRDGGNVAAARSGDGPPENPDRTPFTASARRTSLRPSPIAATHGISTLGSATSVPSVLPPGSLPGASTGFDESESSLPYMMVEPMRGGRAVGSSTSTSTHGSRRLRVVVEPKTALNECWCNDADLPGHMHTPTAVQQAPTPPSVSRIPDSPTPKGLLKRGNTWLEDRLAALATLSTTAPAPASVTAVSYTGMPSVAAAAAVAAGKPRKRSGGTAAGADRSRSAKLGGTPSGGGMQHLQPEALQMVNQYLRDYTFGKKGSGWVACVGRTLLSVATVLLIFAPLAPLAAAAALLAALVIIIHGVMSIVLRLARTCARFISYSRVSGGAAASLPSRSDQRTEPLRLSVSLFLVLHTHVAAWVICGPLGALTGVLYGLGYLWGKDAVPMPWVWLSCNSACLAGVLLVLLDLAFQLRSWERGVCSYPYAVVLRTATAAPDAAAPAAGTVGTRTGGRNMAAAADTGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM13</accession>
    <name>A0ABQ5SM13_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046263132" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="65"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="382" mass="41023" checksum="D763B5C30B3B8A47" modified="2025-10-08" version="1">MPRPIYSWPRGLALLALAGIFACQMVGATSFEHVTRRVLQTGPGINDNIRKGMGWVVTNFPLRAKVSCDPTFYHSPLALNISRPDRSSFCVKITSKMPDVVKYTCQQPASAGQVNRVQVLVAAGCIDALAAVTVDGRPWSNITYDNASNILSIDRIASLPSPDPANPADHTLCIALRPDHDTCGSLTSFCAPAYGPQPSCAIMIADNKGDCCPVNRVSYTGAALQTRLLYRLVGRFLGGCETIKSDADLRNAFATQYRVALGLPAGRIQFLSYTCRTDRMVAIFSATGLKVDEPEQIAERSAALLKPLTSLLGLVGVRASYYEYEPPSPPPSPPRRQQPVRTAPQVGRSPPLSQQSTPSVPIASSPSPPPYGSQSPPVYGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SND1</accession>
    <name>A0ABQ5SND1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015958</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="1">
        <location>
            <begin position="300"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="364" mass="41264" checksum="A3B2FB9A6F816401" modified="2025-10-08" version="1">MDFVVALPKTEGGYDAVFVMVDRLTKMVHLAPCTSSCTAEQTARLFFDNVVRLHRVPKNVISDRGGQFTSKFWEALCKLVGMLVNLSTAYHPQTDGQTERTNRTLGDMLRNYAGRTPLVWDTYLTAAEFALNNAVNHSTGKSPFFLNYGFHPALPVWRELEVNVPAAKLFAKSFMSRITDAKSCLDAAQQRTTEYYNKSKQDVTFSEGQLVLLSTKNLRSLADGSRKLLPRWIGPYPVVRMVGNVAVELALPADMTIHPTFHVSLVRPYRGTEPVPEPANAPAAIEPVPKTWIAGKQKFYDVERVLDYRARRVGRHRRKRTVHEYLVKWTGYTDEHNSWEPARNFSQDMKPLLEEARKCATQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNH9</accession>
    <name>A0ABQ5SNH9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37028:SF4">
        <property type="entry name" value="ALMS MOTIF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37028">
        <property type="entry name" value="UNNAMED PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1182" mass="129970" checksum="CF435BFE8269BC3D" modified="2025-10-08" version="1">MTDGLQGQAGQRRPPVPSVGISIEPAGRGPTYERVQRLLEERRARAQAQPMVRVQLDDTEAAPAPAPRGRRTRPFSSPESDESFAFDRAAYASDGGELGAAGSPTHQRHRSEASGPYSQDSSWQQDAQFGDQSGDQNADEGIGTYEVGSSDEVPGPSMQQRQSSLPYGAEYPIDQEDAEEQEQPRAHDTRMRSDGASSEGGHTLSELEHMLNQTMQQFNIEEMRVPSSKDMAFGIAHGDGPFDISDIWAVESQPSGSHQSASHCQASPSVGCMSRDDGHEVNSYAATSVERASGSRESSYANGHEHQVPHLTVRDWHAGSRPGSAQPRLQSPRHRGPQQQQQLMQQRGFGAGARTGGRIHSAGGSPAPHRRQAALQPPPKQQSLLAQHGAQQSKAQPTQHAHIARGAEVDPTILRRSSTPTARFLGQQHKSAQSGGVTPHYAQPLRSMAVRMSPGRPAEPSEGFTFQPSINPRSRELAERLLPVDRQTRLEQLCKPRNERVEQRCHQLRQAKEEEELRDCTFHPQIGRAPSAPRLRGGQPVHERLYSTKPAWQLKREEILREREQAVLASCTFQPQCGSRQIPDDACRSSSATRRRPASASVISGGDGIFTSYVPIHQRVADLLRSRNEKLAKAQIRMELGSGSVTFQPEINRRSAQLAAERQRQQPAEVAALPASERLYRLAQEAAARRRETGEESASAMRDVSAPRDQRNPFQQQQPLGVPAINPRSRALAESSDLPQDFLARQAYLAALGHEKRALYRSLLEESTCTFQPQLLSRAGSLSSSCGLGITPRGGGGSHWSYSGGDGGWLSKLAYEDVQRSAAVRDALEQHHYGQFTFTPAINPRSRRIGRRHSLTDLYRDEERQAKLERLAAAAEAERAVECTFRPNINQRSASLGRQRRGSLVLASVDGHEQATKLRSSILTEARALKEYEELKECTFTPAINRTVPKPNGPIAVPGLGRHMELKELARKKREAEEARKAEVWNLRPKSPGPRAGITVPRPFSFEARVLPWQVSRAQLVQEHQDKEVQQRKALAALKQQRLQQRRHAEERRSLQLQKILLETQGSGSHAQQQNTRHGAAGPFPALMGPPPETQAALNNVAGGGNGGRRRSVTFLSPVSGEIYGPGSSRGVAEMPVDADLRQPDFAPDAGDVCGEDIVIGSYDDFDYVGEGVVEEEVSVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNI3</accession>
    <name>A0ABQ5SNI3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016716</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046691985" evidence="2">
        <location>
            <begin position="17"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="407" mass="41634" checksum="AAAE59E714456CF6" modified="2025-10-08" version="1">MLALTLVFSVLPLVASIEIPPVFGRNDNIQLLPASGGLQPGAVIHTTPRILQGGVADCVWDGAQCNLRRDFVCDLPGILGSPTTGWAKILADAARRGAVCSNFKGQPDCLLAAPSLDCDWDLVLNRCTLADILDVTALRGVAYFPGSSLDTAMSCFSLGPSACSSSASCILVNASQLGTSSVARGLANLGDLRLSVVMDSMLGTMVKYGIAGLQDSIRTVITSLLGAASEVPDSGQQVCVSRWMLDTASMTSLAEAVADAIGRNQDLLTALVSSTSLTGSYNGTSLPLLVTVRECIAAASASTCAAAASGRCQWLTSIGACNIRENLLPSIALDPSDRWVAVFNNATALCRSITNASLCAAAIDIVAGVQGGGPSGGSGTTAVTITWCLLAAATAIAAALSHLVREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNT2</accession>
    <name>A0ABQ5SNT2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016869</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039873">
        <property type="entry name" value="CCDC78"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22106">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22106:SF5">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="535"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="790"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="617"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="700"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="564"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="584"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="790"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="855" mass="89522" checksum="0A8811BF51FAD6CB" modified="2025-10-08" version="1" fragment="single">RSASGAEAPEYQAGAENPSLPEGEGAETAAAAAEVDVGAESTNPVDRVSAKIAGEKGLDRQIEILQSELQSTRQELESVRTGALGNQVSTDEVRETYRRKLQAMMRHMAALGLSMWEEGDGQGQEGADPRVTPEALFRALQSLADENVKVTEAREADLRHYADDAMARLVELRRKFKALYTAYRDLRYLVEDRWPGPDPPPKVPHEDEIVGGTLEGLIRSEEEADRRTINRLRDQVSQLHALLESMRLSRGVREAKPTDPGVSNTAPTQGSGTVTPWTSTEGLKDITTAPAGGGSEVAATRAARPPASADALSGPYRTGSARVATPADTSTAKAYQTGDATASVDTSRGWTNASSTSGVHMADDPRVSIAPSPPPIPAAAAVYPSGDTSRPLTPAAVLPTTPDTSNLAPSPPPARPQSPGPEAASLPRTRTTTRMMAAAPTTSPPRSPPAAPFSELDGSFPAPILLPPEVPEPVVGPPPPPPPVAPVALELQTLPLELPLPPPPPEVGSPEGTKAKLYGAVVDLDLDPGGVAEVVTRGTADGSSPVKQQPLQVQVPPPPPLPPAANAHLQPSQSPLALQQRLPSPLPSPAPPRAAPQQASGGSRVAPEPDPHVQVDNQRLREELGQLKKRLQAVSEFPAALEAENQRLRDEVDSLGRKLQMAASAASAGASKAASAVAMPPNTVNEGLIRQLREFNQKTTAELQRRAQQSETRAVMAEEQLEQLQGYMTKASATYQKEIVRLRSIIGQMETSIGAVKGSYLNLSPLEGVGGGGTLRPAAAVLEEIASRNGSLTRRTSAQGQMVATTTPRETRSGGGSRTPLISPGGGLLAAAAANAGGAAVRSKADDGVTHLPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP62</accession>
    <name>A0ABQ5SP62_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_017201</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000125">
        <property type="protein sequence ID" value="GLI71808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF376">
        <property type="entry name" value="EXOSTOSIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047088471" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="25"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="195"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="651" mass="73959" checksum="32EE803E0EA59739" modified="2025-10-08" version="1">MLLHRSLASLLAIIFLHIVKQVQSTETVLKTALGDHPFNKFQEVNKPCQEGCSLYGNCNHELGVCECPFGLSGPTCQTQLYPACRTVFTSGGQRIYLGRTAPRNCYCYQQLLKGLCLPDDESRCAFHTMQLWDSLYCFQRKETPEEQQLSELPERIDDPTYEWGRCTTSRTGTTQKHVGLIDRLPTIEDISKSVPLSRCTARCYEQGVCQDWGGQTRCLCFAGYRGEDCSVSEMAEETFCPDTCGARGKCIAGFCKCDAPYWGLGCSRSKAYEPVPGSIAYPYFPSLKIYVYDVPPNVVGPHQFEDGDINVFYNYQSFMRFEEMLLHDTNGVRTENPYEANLFYIPAFAYYSTSNLGDPTGAVVRAVDWVASTFPFFNRTGGKDHFILISADRGACYLKRVPQTEHLIRVTHFGYERQNITDMGQLLHNTEYGCFKAGRDVVMSPFFKAKVPKIQELRARLEQPAGLEAFIAKKDVLFFFSGDIRHNEPEYSGGVRQALSLLLSNNSYPDVVFKGGYMMLGGHEYEDLLTRSKFCLAPYGHGFGIRLTHALMHACVPVIIQDKVRQPFEDILHYPDFSIRVSKAELPRLVEMLRAVPEEDLVRMTKENSRIYRAFLWQPELGGLAYNFTLASLWRRVSHFRGELYEAGVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPB2</accession>
    <name>A0ABQ5SPB2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007657">
        <property type="entry name" value="Glycosyltransferase_61"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961:SF38">
        <property type="entry name" value="PROTEIN O-LINKED-MANNOSE BETA-1,4-N-ACETYLGLUCOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045984524" evidence="2">
        <location>
            <begin position="20"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="797" mass="87184" checksum="736F41F0B30C3178" modified="2025-10-08" version="1">MMQRHLLFLLAIGLLPTQSQRIHSFKNWATLRDANWSGLLHEPSAFVVKSHVRLRNRPEGYQPGASCLKFVGMGYLDHIRDSRQVLCGIGRTVTSKDIYGAGDSRSHVEPTLSPSQSSVECYTAPYVQVGSSNKATVSLCRSRNLVMNTCAFLAGRRKGTLNKKFPLPAQGAVQLGCQVIDYVKRAESSSDLQWLKSIHNEVWWANATRNESGVAKACASGSKTLVTTPTMFVMRDRHANYAHEMEVVSMAFSFLAAVEPADVAAQGVQVVIVDQAPPTGFLETWARISRPHRLRILAHDPFPPNTCFRSAYHVYTFAAGIGYNTNGETATCESPVTMGMSHWLRQLYEERDPAVPVDLQGAAPMMAAATAGPVAAAAGVRGMRAPTSGIVMKNVVWLSRRNLEMVRLLLNASVGWKSMRMVRNEDEVVAGMLAAVQEWNSESCLIRRFDQNVKKAYEESLHTVNPVHIGPGADSVNVANAGAARRRTSAESPRIRKARQQRKLLGLAENDKNDERTDTNIDPDPDILGWDLLEYDSEGLDLDLGHTSSIDGDADINRVGRAMLAVKAVGGSDIDEHGNDGDDEAEEQDRAFIRGAGDGDQGGDSGWGDGGPKFKAGPKSVSSAKALPMESFWDDTTDLGDCRRTNVLFTFVDGDFNDMPYHQQLKIIFRTGVLVGVHGAGLTHGYFMPPGQSAVLQLLGDSFVKVAANNVFRNMAVGLGNFYEDVLYEGVDVEVNVLKQAVKRAMNFVADQVMDAQLKQNGGVLPPLVLDDQNHFSIVVPSPLQCPRNRSLTWKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLL8</accession>
    <name>A0ABQ5RLL8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="123" mass="13061" checksum="CAC10F0E105F71E4" modified="2025-10-08" version="1" fragment="single">LGARLRSNDVQLAEVLNSDLLIKLVTLLGRSADAVLVAVNAMEPCYTEPITPEGLGSGLRSTGLTYLVTRKVVADLRAAVPLLLQLLINLASGPLEGLQVLVRLGAAAAVKQLMRWEDMKVGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM15</accession>
    <name>A0ABQ5RM15_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="131" mass="14189" checksum="4547584DACF19534" modified="2025-10-08" version="1" fragment="single">HRMKLRSRPDVARLEGDLLLLRAYDMKLVDGGGGVMVDNTVTVEERFESGGFFGLASATMRKAGSVLLLRLLAWKRVGLCSKSEALKYAFVLGARGAGRTTAFASLVNLSVDYQVPSSVPAEIRPIPRVHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM20</accession>
    <name>A0ABQ5RM20_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21453">
        <property type="entry name" value="DLC-like_DNAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF2">
        <property type="entry name" value="DYNEIN AXONEMAL LIGHT CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="103" mass="12063" checksum="51EC866A52B4B07E" modified="2026-01-28" version="1">MAEQQFEDMEAFLRVAKYTLVKFTDMNVEMKEEAMDICITAVEKYPNDAEKCTQMIKDQMDKKFGAPWHVVVGKGFSYEITYEVRNLLYIYVGGRTAVLLWKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMH6</accession>
    <name>A0ABQ5RMH6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosome subunit biogenesis protein NIP7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000276</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with pre-ribosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the NIP7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21146">
        <property type="entry name" value="Nip7_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21151">
        <property type="entry name" value="PUA_Nip7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="NIP7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055359">
        <property type="entry name" value="Nip7_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016686">
        <property type="entry name" value="Ribosomal_synth_fac_NIP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="pre-PUA_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017190">
        <property type="entry name" value="Rbsml_synth_fac_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUA" evidence="7">
        <location>
            <begin position="95"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI58534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="179" mass="20255" checksum="0046850AD627C213" modified="2025-10-08" version="1">MRPLTEEETKAVFEKLYKFIGKNIKNLIDRPNEPFCFRLHKNRVFYVKEKLMKKATNVARDKLVALGTSIGKLTHTGKFRLTVGALDIISQYAKFKIWVKPSSEMQFLYGNNVLKSGLARITENTPTYTGVVIYNMADIPLGFGVTAKSTNECRSLDPNAVVAFHQADCGEFLRAEDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMH8</accession>
    <name>A0ABQ5RMH8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glucosamine/galactosamine-6-phosphate isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000528</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF0">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="2">
        <location>
            <begin position="95"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="361" mass="38146" checksum="C6474DA384C85498" modified="2025-10-08" version="1">MAATIAFQGLKWPPACRASLRLPASSGTTLHVTAYRCNPSRGARALELQQFQGQQSRQLRKASRNTSKCAALISYVEVAAGSDYPEDLAVEVHPTEAAVGSRICELVVHAAQVAVAQRGAFFLAIPGGSVLKMCGGLVGQPVPWDRTFLFYVNHKCVPLGDKASTHQKALDLFLMRVGAPLANVAALKGFPFLWPQGSSDPVVEAREYESWLRQIAVQRGMDLTSDVVPRFDLMLLGMGSDGHVGSLYPGRSETSITGPGAPLVLPVDKKQPASITLSLPVMCAAKQVVIAMTGAGKAEAVRAALQTPSPFGDSIEMPARRVRPVEGGSALWLMDGPAASRLDVAAGARALLAADNAARAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMV9</accession>
    <name>A0ABQ5RMV9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048821">
        <property type="entry name" value="PDE12-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF37">
        <property type="entry name" value="2',5'-PHOSPHODIESTERASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21171">
        <property type="entry name" value="PDE12-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2',5'-phosphodiesterase 12-like N-terminal" evidence="3">
        <location>
            <begin position="250"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="450"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="824" mass="88402" checksum="9AF4AE0D361FAF57" modified="2025-10-08" version="1">MRALVRLHTSCRPGRHFTSVDLPGTGALVPERLANTGFGAAAAVIAADSRQRTAKHTVFTHSGHQKSFRFRSLQFDFSSWSTTRTKGSGSSNTGCCCRATTTHIQAPLSASNTIAMDGAPDFPRAQPKAYVRAPPGGDTLVVSISLGPKTCNLKRPTDEPLDKALTRLAKNLMTKPSKKDKRKKLGSAEVAVGSSGTDTSMDDARQLLAVLYEDAAGNRPLDRSLPNGVAWMRGALLEVGGERFVVEVNPPTVDKLSIDRLAMVGYPIAAAVMVSYADPSACRWRWTAWRPGGSSPDPKWVDLGCNTQHYTPKPEDKGSVLKVECTPVAVRRRHQQQQQPPATATGSGGGGGDAELPTASAAAVEDGDGEMWLGETVSVMTGKVQHGPQICSATVRCVHTLTPVQPPGFRILSYNVLADQYAGSDYAQNVLFNYCPKECLDPGYRRPLMLRELLGYQADVMCLQEVDEKAFSDFLTLHLGLQGYEGHYTNKQGKVREGSATFWRTSRFTALAHHDIRLREAFTRPLPPLHAQFEPLLAASQELTAALQQVNTIAQATLLAPREGQDEGGDGGCLCVVNTHLFFHPYAPHIRTMHTAAILEEAAAFIERCAADPALASKLRGRRPTVLFVGDLNSDLNDGIPGVIELLRRGKLPADYWDWVQGAAFKWGMGEEDAEAMAQGTAQIAAAAAANAKNTGAVAASGDGAVAPATAIGVESASASLSSLFFADGHAFDSAAASPHSPIAVTGIDITSPFTLRSADDLKTPYTNFTSGYDGLLDYVWYEDPALRVVSSVPIPTEAELGSYIPSPAFPSDHLAVVYDMEWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN18</accession>
    <name>A0ABQ5RN18_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000590</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13681">
        <property type="entry name" value="SURVIVAL OF MOTOR NEURON-RELATED-SPLICING FACTOR 30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13681:SF24">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="371"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="558"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="773"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="935"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1104"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1349"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1675"/>
            <end position="1780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1828"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1984"/>
            <end position="2008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2127"/>
            <end position="2216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2264"/>
            <end position="2296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2412"/>
            <end position="2440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2463"/>
            <end position="2490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2614"/>
            <end position="2688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2739"/>
            <end position="2773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="371"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="579"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="595"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="781"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="935"/>
            <end position="963"/>
        </location>
    </feature>
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        <location>
            <begin position="1135"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
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            <begin position="1361"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1699"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1716"/>
            <end position="1730"/>
        </location>
    </feature>
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        <location>
            <begin position="1738"/>
            <end position="1747"/>
        </location>
    </feature>
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        <location>
            <begin position="1856"/>
            <end position="1866"/>
        </location>
    </feature>
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        <location>
            <begin position="2127"/>
            <end position="2143"/>
        </location>
    </feature>
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        <location>
            <begin position="2150"/>
            <end position="2163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2179"/>
            <end position="2209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="2271"/>
            <end position="2284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2427"/>
            <end position="2440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2463"/>
            <end position="2478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="2657"/>
            <end position="2686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="2740"/>
            <end position="2752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2858" mass="296873" checksum="25BA0533E059D1AC" modified="2025-10-08" version="1">MTRHTKRARKSADDFPGSGRSHGGATCSQQRIVDLHVDLFPNITWFLQPPDILALRETCKFLCEAIDGSRTHIVLHLAHVQQTIFLDRCRRVESCNAYVNPSASEHLPSQSAAVDASGPGRVYPSRRPPSPVPPLHGLKVKPYHSCILSAFDKWPRLEKVYVLGDSPESYITRLVQDAFAAGRLADLRRILLRQTVWRNRLNATAVEALVICESALLGVISDSEAVEQLEKLDLPLLPMPLQPDEVGAAAAAAAPAAGEAGQEDDGELLPPPPANIPDQRIREPVAQSLIHWLVHFAAVLSYSGSYDEAARVARLLWAPPLADAARATLAAAQLARERPLDAARTVLEMQPWHARALAAIVMEIIQQQQFNQQQKQKGKQQKQQQRPQDDGHQRMPAGGGDTRHGSPVSQPVPEASPPNDAGANDNSGHEGVGGASGGGGASTAAVAAAMASAPPDVRELHSRLAALAAMDGGAGIVGALGPQLGAGFGLDAVLLGPPSPLVLAFAQAVTACAHGIPDTGLDLLHLHWLRFQVHAERQRHELLQNTVRLQRQLAELRSKKKRQDPAVLGDGNGSDDGNEVAKDEHEGRNDTDAEGGVGGAGGGGDGVDGDSVIAGAAEGDMGWRSLLTLQQLQDARRELFTLERRLTIEQEDFAAAVNEYVNKHVLPAGRVHRYSACWSAGIDDVAVAEAAAAAMPLPTSADVIASMAAPVAATAADVAAAVTAAAAAAAAAPHSLAMPAGSAPQMRGQAATALNAQADVLLFLIRMVYKPGPQSAPGSARSPQPDNNTAGPSMMPSIPPSPPAAFFYGGTHRLMSSSPPPSSSPSSRWTFDNCIRETLASVAALLLIGSGYVGAATRVLTAVPLTSHLQRASLLDRMWALIGANSTWIRYLPCHGDVQDAAGKQVSLGGNELELETALGSRVNFLESQRFWLLGDEEEDLGEMEEEDDDGEEEMEEDEDAEGAEGGHEVEAAIQQAAGGGNGEQGGPDAEGQGNGDGGGDAEEQAAAAAEAMDFEVEWEVEVEEEEEHAQAALAALANAAIGPNGGGDGGGEVDMGGGQAMAMAVEAGFGNPDVPALVGEAMGADVQLQQGQQIEGWNLEQLLQPPQAPPPQLPPEHGAHGLAAPPILQPPPQQQQLLQQQHQQQEAAAGAAGVAAAPAPAAVPMPPELPGANLVSEQEEQLVRQLSRGVQYLWSASTYSGTSDVSPSHFPSGDLCIRGPHPCRGFRSQPELVRHAEAVMEAVRLSCTKDVAAEGLQMPSLALRAGGAAAPPGSNEVWQTSLATLMAACASVAGVQRRAMAMVMAMPDVRARLAAAVAVAAELVQQGKTSLAAELLLTAYGTPPHRCSSNGSEAADAMDGSGGSGSGGGDASSFVASSADPIESTVTPLSTLDFKMTLSGQSALVWACAHLDRVVHLATGRARVCAVASVLNSQVASKCSSSIPTTVVAACSRAHSRPIFLNACAAFPGEEARALVPLMRSLPATFLERCLTEVPLMLQPTDVARQLLLTSLGRADPAMGREDMALALHQLMYYSKLSVGTRVGLRSAVDMSPLERELTGVSLLLTSLIRFIETPTPRALTITGAGVGIGMEVGVGVGAHQGEEEADGAAANVLAGWLGQQQPQHPEQEMEQEDAEEAIAMEGVHEQVGGQAAAAAVAAGIDLHAAHLAAALGPEAGDEDPSGLVLEAGGGGTQGIDDMAEWEDMGESEDDDSDGEGHGGRETGTEVKRGRGGTDPGSSSRSVGSSCPAGGKGEDAEDTDMEDRPAAEPGPEGMAGGAEGPALLSVVPLIHLDSQAVRRLRLAPSVLRDLVSEAQMWGIMPRLRCGSGPGGSADAAEAATDPTVCGGGGGRTDAPTATTGSSTHTAPGRGSSACISWRRWPPELIACRRLPRLLADALRPEDAGVLGPLLARTTGALLSWDLPYAVVLAVHAARDLGPEALGESGADVAVSSLERLCQACSRYRFVRGMLCRLSSSELQPPAGAELAGDVPTATASTANAGRGRRRRWQEVAVPRRTTGSRGSCGAGGGVPLQGWRLPPPRTSWCLAAELLVHCFAPYWRQRLSAAVLGMSAMVVGLPARDRVGPMAQLFLLAAVGAASLAPASTPEGQQRKQSAVALRLQQKLQEQHKQYQAEARESDKDLSAPPPAEAAGNLGAAGLLPASGRPSLLTLPIGSSEGRPEASAEERPKAGDATGRLETREVHAKEASRSAPSVGERDSLLHMLYDVAHPWQRLLYDLATAVWGDELQLPPAALSTAAAAAGTSGTVNPLPLPLPQQPPPSPGDRPSSQAATAGISVAEDTVRDPAASRHLLDSGSGLCEPRTPVNTSEWDTGAAAAPTVAAAMPVGTGRFRASLAAVRSSGRSAADRQPLLPLSLHLSDGLEACFADNDPLTRAVMLLTTIILAAMDLPSRRGDRLGPGQPIGPQQQQQQHQQQQQQQQQQRQQQQQQQQQQQQQQQQQQQPDQNAQLQQLQQLQQPGGEGGPAGESVPLEAAVEGSQQHLEGLAWCHGTRTSLWVWEKAVPRLQKASLLGLVKVLKVPKIVLTTLLEGLLTLYLPRKLRPSPAATGTYTASGCAEADLHSQAGPSCADAGATVAAAAPSAALTPTAAATTSASGLTHGTGVQESAAHDNIPSATDNAGVEVCTSRGVQTEGADGDGSSGSGVSGKAGDGGRSHGNGGDVSGGGSDDDQLSRLMFLLMARLVAELIRDGYPRGPPPHMPRLEAALQRVRADAADVVHPHPHLHPHPRSQHPQHPQPPQHPQEEGQRSPACGILGKVGASSPSAADTNTVNTVLRGAGAAGSSAGMGSGSGAWRSCGIGGCRLCERILQVEQLLSEMGWSGFGAAGGSGDPSGSGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN28</accession>
    <name>A0ABQ5RN28_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044638">
        <property type="entry name" value="ALDH7A1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521:SF7">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 12A1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="125"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="546" mass="60105" checksum="61D35CEAC85CD23C" modified="2025-10-08" version="1">MLWHQARPLGQAPIVLRPLAQLVCNLSRWATVDPEVMNSSAPAECRNLVGGRWLPSAISRQLPDPLTGDKFLEVPHTQPDEIGPFVQSLRSVPKSGLHNPLKDPERYLLYGDVSFRVAAELRRPEVEHFFARLIQRVAPKSIVQALGEVRVTRKFFENFSGDQVRFLARGFTNPGDHPGQISSGNRWPFGPVALITPFNFPLEIPALQLMGALYMGNKPLLHVDQRVSVVVEQLLRLLHHCGAPPTDVDLLHGPGTTMGEVLKCAEPRSTLFTGSQRVAERLAVEMHGKVFLEDAGFDWKILGPDVRDLDYVAWQCDQDAYACTGQKCSAQSILFAHRNWVIAGLLDRITDLATSRKLSDLTVGPILSWTTKSILDHTKRLLQIPGAKLLFGGKPLSGHSIPEVYGAVEPTAVLIPLEQALLPEHFGVVTTEVFGPFQVVTEWQDGQLPQVLDACERMSHHLTAAVVSNDINFVQHVLGNTVNGTTYVGIRARTTGAPQNHWFGPAGDPRGAGIGTPEAIRLVWSCHREIITDFGPVPPPAFLVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNS1</accession>
    <name>A0ABQ5RNS1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Multifunctional methyltransferase subunit TRM112-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRM112 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21089">
        <property type="entry name" value="Trm112-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039127">
        <property type="entry name" value="Trm112"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773:SF0">
        <property type="entry name" value="MULTIFUNCTIONAL METHYLTRANSFERASE SUBUNIT TRM112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773">
        <property type="entry name" value="UPF0315 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="13997" checksum="9F8AEBB062311D74" modified="2025-10-08" version="1">MKLLTHNMLSCHIKGVKNGYPFLIEVIKVSEHEADFDPDFLKHIFPRINWLAFLQGAEAMGCREGLPEAPTETYLEDEQFQKAFHHALLEVTLEEGSLVCPETGRRFPVAKGIPNMLLNEDEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPK2</accession>
    <name>A0ABQ5RPK2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chlorophyllase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017395">
        <property type="entry name" value="Chlorophyllase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428:SF14">
        <property type="entry name" value="CARBOXYLESTERASE TYPE B DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428">
        <property type="entry name" value="CHLOROPHYLLASE-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07224">
        <property type="entry name" value="Chlorophyllase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="26788" checksum="B799D4C06D930603" modified="2025-10-08" version="1">MFNGFQAKASWYTPTANHVASWGYVVLQYTLRGLLPIVVDSVELGYLTPLLDWLKTQANTTPSRLYRLPNTTRLATMGHSRGGKLAALHYAGRSDIATAVLVDPIDNTKNTPEGPDYPSAAKALMAANRSAGLIGAGVISSCNPAGSNYPIFFNALAPGSWQMVVVQAGHIQFASGDGSLAFWGLDKPCTHGKITTKEVVDDTNTFAVAWLQSTFRKQASQTGLAAFHAWVQVQANSRALNFTVKASATI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPK9</accession>
    <name>A0ABQ5RPK9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anticodon-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="41106" checksum="D9E93AAC37AF7798" modified="2025-10-08" version="1">MQSWPVCKRLARMDMRIMTHIRASGKGSEPPVATPLDLVFKKASRKVKLVHPAYQNPQRERKKEVSKQPVARPVASRTKPQNRLKPQQMDDDALFADITESERDSVWENIKLAIEEDSEGDEEWLNGPGDGVLPQQRRRAGPGDHHHGPAAFFRRELAIAEHVSSSSGYKGFGVPPRRGEPGPRAPVSAISRPSSKHAPAAPVIPKSSPQRKEAGPLQAGSTGDRGSSLRLFLFNAEEARTRALLQQLGLAARVQVVDDLRCADAVVAAKLAKSGKHVNLTQPLYNAQGERTAANLGIPFLVVGRHMTAENLNGALRPLLDPQPEDAAGASQKQSSRRGPTRAELQAHRVEVVHGFAQMLREGALTPAPAAGARGPEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPY1</accession>
    <name>A0ABQ5RPY1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LMBR1-like membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_001552</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051584">
        <property type="entry name" value="GPCR-associated_LMBR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355:SF0">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="589"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="662"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="720"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="598"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="720"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="764" mass="82025" checksum="063F53A14C4AEE95" modified="2025-10-08" version="1">MWTFFIFSMVLVFGAILLLLRRYCANSVVLSVKVTTAYAWLTAMAVVAIVPLDVFSTLGNSKPSELGYMWNVAFWSTQILTWLLLPFFQYYSDAGDFTVKGKCITSLKENAILYGSVAAVGLVGIAALLITKKMTFDGLTGLGIGLSNAFGLIASIILLGYGLVEIPRNLWKADPERQLKWCAHRAGKYAEKVMKSTAELETVVTIVAANERQMRRHDPLRKYMDIIVEQVEKESPIKPSDLIARGTASGRSSGGIDIESLNAEDLEYNYDVAGLAALRRRMIYAISTYKGDRAQYEEVMLEAFELEDVVKCRQLNDFTPRKPTRSPIKKAVWFYKCALRASWHRILAVVFAVLSVLIVWAEATIVSPVDISPLSLMIKGSEASEFGVQLLTLLPLAYICACTYAALFSITAFDYNKLIPRATIGSALMQNGTLMCRFAAPTCWNFYHMIRMTRRDTGGTTTVFEDMMGSMDVPNVLRQHLNTYLPLILVVECIITALNLWDRIMGLCVSSKYKFSNDDDVDDEYTEKGRQLVAREREAVEKGFTIGQVLTSAFFDLDFPDIVGGPRSRLTQQKKGFLGLFGKKSAPAVAAPPPPLGAPQRPAGSPAASVPSASAPSGTVPRYKSSTAAAAAARWLGRGTGLPDDGAPLAASASDMLDSSSAKASLLASRQHDKSPTAGEYDAPGQTPGLDNIFATLSGGGPAPSMAGAAGGGGILRGGAGGAATAAGGAGGGAGRLGGGLSNVPGAVQPHSEKESLLSGFWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ56</accession>
    <name>A0ABQ5RQ56_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="233" mass="20805" checksum="E1AE472F4F9CFF34" modified="2025-10-08" version="1" fragment="single">VSLAGPAMVAGPAGSAAAAAAPGGVMGVLGAASNNVTIPLCPVPLGGLNVGPGLNAVLGGLTGLNSLNGLNPAGAGSGLNGLGPLAAAATAAGLNGIGSLQGLTAPLAAAGLTAGFGGLAGLSGLAGTGPLGGLPPGPLSLVAHSKPLATLLSSGGGGSGLGPACATSSAGGGQLLPTQGATSPSAAVASAAAQPHGGQQQQPPAAPAQPQLGQSSSAQTAQISRLPTSASVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQM0</accession>
    <name>A0ABQ5RQM0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001833</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052993">
        <property type="entry name" value="CFA-57"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="696"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="815"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="964"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1048"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1188"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="295"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="469"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="712"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="815"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="829"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="881"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1048"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1074"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1101"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1128"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1212" mass="126982" checksum="4A02127BD03155DD" modified="2025-10-08" version="1" fragment="single">FRVPGRASLVPIGTEQGGPGAATHRGGKGMRLQSARGDPLSGGGAGTGGSSLAVSELKLRNSYSSIIQPFRGGLTIARCSTAPGIGPGYAMILGTGVLGRVRMCPLRPRSNEDVDEYYLHAADVTQVVAHRDGRFVFTTDSSGCWMMHVLMPPGTPASWGSAATAAATAAAASGGSAAPAGSSAAAVSPMPGVVASTGPLSPTRATSPARQSVPGGGGFGATNRSTSGLGLVTQLGTHIELTAAAHRLMQLIVSMGGLAGGRHATAAAAASTDISVSGGTPVAAQLIVSVRAVDLQNLKESQRELKDRLAKAAMEAEYKMYEREQGVRREFEGEVSRLRHEVEVLRADLEYTRTTASDESNAAARLKAAMTRDFERALREQQDMFERKLASEISRTDAAEREMHRLRAKFGRKLWQLDEQQGSIVRDAAEKQSELEEVIESTKMEAQKAIAAAHIQTEQELRIDGELNEEELQRAADAAQKQLEERDESYMKLLAKHTLQSGLNAKTTQENERLRAEAEALRKENTRLAEQVMALTEELEAMQNAWADRDARQRHQDAEMKDLAFQWQQSQLFSTLATSRIKELNNELEPIKAGRTEALAHVAQMESVAQRQLERQAKVVSENASLQSRLDAAYEELKNVKGEMLEREAYFKNFTTQLFRTIQDVPHMHWPKLFGRLIDDYHKGRDKASWTRYLTADHGSNAHPPEREENSRAGSAASDAASTSAEYHEKIAELHSQLVHTEKMLSLLTETKDKADKARRAVVSKLMADNLMAVQELNDVKRDLKAARELAEKRAIELADLRMSLAVAGAVPSQVSSHSSTAQVQLPGNPGGGGGGGGGSTAAAAGSTGGDSLPQGPAGRAMARLPSRFGDPTRNIAAAAAGGGGGGGGGGGGGGAPARGDGPVTAGAFSPIPEHSISPPQPAMVVTGDMPQGGWWLGDNSPFELPSSLDHLASSTLRQRSINSSYDGGSAAGVAERPGTGGLLGRRPATSASTGPGGGRMFGPARNALTPPTPHALLPGRSRPDTSSSGSLVAAAVSGAALQAFKIQQQQATQQQQHQENMRPISPAEGSGGTAATASAGPVLQRAASPPQAQPPRPEGQPRATSAGPTSSSAAAAAAAAATVGSGLFQNHSNSRTRVRSTSAVPTLSLGSSPYGSGASLLPPGVTMPFLQGQVRPSTTSKVTTMGAGFRSFVPTPGSNAPGSRNGTGGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQR5</accession>
    <name>A0ABQ5RQR5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001581</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="93"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="485" mass="51169" checksum="C76C4BC383629509" modified="2025-10-08" version="1" fragment="single">SVIGLPEGVGGLQRSNRSFCLAALATLKLSRAPVSGRQMGTNVPRTRKALILLFAGLSGLYSSICGVLSEETPVRRNLNLLVDDVTKPFKTDFPYCVCETYDCGCSPYNLAVKNVTTDLNGTTRFCFSINFVGCNTSLPCCQTLMKRVDKITLTSSASCPKASISKVEVLGKIWKNWEPKAWILPSGGLGYEFRVYSLGASTQAFSKSDLCITAVAPCSSLTQLCDVEGGTCKFSLSESPTTQYCPTCPLQPTPSPPSPSPPSPSPPSPSPPSPLPPPPSPLPPSPPPPSPPPPLPPPPSPPSLPSPFPPFRPIAPGFSRPPLPPWVCEVCASISVTNLPPNQTQFNSSMCSSAPLRADNLTQSARAAGVALIQPFKVSACMEAEVRVCGILANSTDANRLKLGAELVLEDWLSDAVVLCQPGWQIQATMGSSNSCISISLEQACAQSRENPPFPPQLPPSPPPPPPPPPPPPPPPPPPLPPPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQT9</accession>
    <name>A0ABQ5RQT9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sec1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="851" mass="85281" checksum="A0B86E451B4C905C" modified="2025-10-08" version="1">MFEVIQAPVLELAESIQGSLLYLDVGAGEIARTTLGLPFLFGLGVSHVCLLESASTEDASFPLLATGSLPNKLTIFTTQLLTDAHQHILRAVLAHPALISVSVYSSVSEHAHACQAASELGVEAYREYAELLQEEVRRARTVGLATAASSAGASSGVPPSSLLSVRVAFLPLLACCLDPGLIVLPAASSAARRAVVGAAAAGFAPPELGAGDAAVHGEGASGALSLTAHGLLALAAAMGAPRPEPYAIGPVSSALAAELASLPSVSVSVSVPPGSLVGASSTASSGGASSSSLAIILVDRALDLASPCTHTDHPWDLILAAAAVRQAKTAAVAAAAVSAATSAGGASPPRRGIGGGHGGVSGGVQAIWRPLDLRVALSAPADAVPLDVPWPSVNKKPATGVVELMPYGQLSYDSYGVDLLDPTDRTAITRVEAVAGRSRRDGLAALRRGLKEALRAEKLTPAVRSKAGVVQAPELQGLSEPLVAASGSCGSHRGLAALGLAMADVMTSPAAAEWEAAAGLERSVLAALSGSGGGDGGDGAGGCEAAVQVLLDALAAANSGRGLLHVGLVLDVLPAVFSAAADAEVPVAAAATSSSSSAATGGGGRHPFSPQHLTALRDALESTILACTTPQEALGGRLPADLVSELTARAAAESEAAGSREEQGPTVPATVAAGEEPVLRRRLASVMDSIFAGLRYAAGAREPLKTFKKVTHVDVFAENHGTLSPLLRQVVRKVLQRAEVGDWQQQPASAVGGGGLVRGLLGGLMGASRVLVGRAGGQAAAGGPLPADCGTVLVFVMGGISAAEVREVRAELDEHVGTGKPRVLLGGTSLLLPQDVILQLAGGPLPQRRWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRG3</accession>
    <name>A0ABQ5RRG3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vps41 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002165</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057780">
        <property type="entry name" value="Beta-prop_Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 41 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23411">
        <property type="entry name" value="Beta-prop_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23556">
        <property type="entry name" value="TPR_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps41 beta-propeller" evidence="5">
        <location>
            <begin position="12"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="3">
        <location>
            <begin position="952"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="196"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="364"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="585"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="585"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="596"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="611"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="630"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1462" mass="153144" checksum="D89C2D94347AE87F" modified="2025-10-08" version="1">MATSLEEPEPQLKYELLGGDVQSILGNEKITCMNVSEKILAIGTEQGRVHVLDYSGNQVRLLNLHKSRVNDLSFDKEAEHIASASNDCSVVVTNLYTDQITRHEFRLPIKAVAIDPRPITSVRKTWRDLVTGNVDGEVTLHTQGWLARADQPLGAASSRDVAAGGVLAVKWSGTLVAWAYDSSVSVYDSISHQTIRTLRRDDPRAPAPATVRGTPPPKSPPPKQHPADRPAPAASANAAAVAASSPAAAKSPGVTVGGTEVLTEGATGIRSLRRCSLLFAPDSHLYVAWSDTVLVARIWTVENSVGSLWPQVDLVARMKLGEAVLGVSPYGDSDLAVLVYPSGLPPSASADSTLQQSQLGCQRKLQNGGRDDDTTKTQDSSPPPPPAPAPAQQQQEQPGRQQGQQLQHQQDSKPREGNDALGGVRSARTPDREGSVCPESSSTESGDAPSPSSSPATRNERRGETLAEGDGATRTVAATTAASLERAAELPLPSPPDPLAPGRTPQLVILGRSDHREWARDSVPLPGADSCGPADYLGLMPSLPHHLHLPATAVDPEAAAALGGAFGSLASMAVAAAAAAVASTATPRPAATGRESSGGAGGGGSGGSGGRASCAVSDSSRGSGGGGGVALSPSAAESTQTTSIPSTPASTAKAGHSTTTSAAAAAAAVTAPSARRLVPVWVDGQELIFFIAAPMEIATARSRDSNDRVNWLLARRRWEEALSVVEEAPQGLPPGLYDRVVNGYIEELLTTQSYDKAVELSARLLKDDATRWEHWVYLFAQARQLPKLVGELPTHNPRLRSQVYDMALQSLLLNPADHPTLVSLVHTWPTGCYSLAALADAVAARLRRLQHQHPQSQPPNMVVAPGAATATAASGTTVTDAGGSGVGSDSLWQLLAWLYEQQGRPDLALSIHLRLKSPSVFIFVNHHGLQANLLGRAPDLFSVDEKAALALLTCHVDALPPANVVPSLQEAMRSAPSPAAAEVWRRRLFAYLQALFAADSSVGADYHDLQVSLTAEYQPSKLMDMLVSSQYYSLEAALAICEARGLVSEQVFVLGRMGNADQALRLIIDRLGDIPRAIDFVVGQRDDELWGRLIDWALGSSETTGALLDCIGGYVDPLLLVRRIPRGMRVERLRDRLRAIIADYRTQTSFREGCNAILRSDCRHLLSKLYDGTRRALPFLYVMRPEGGVDGGRWYRCDVVHGGKLQAVSAAEVPEEALFGFGSYRPLGPSGAAAKSGSPTSSPSPSAAPAAAATAAASNSLHSDLYRVRGSSGVGGVVLGRGSATNLSSSVPSASMMAAAERIALTRGSSLPVLDRSGAAVAPAKEHFPWWAQSGGLDVGGVTRGSSGLAAMADLWVGFNLPSGRARLTSGEETPQHLVGGFRRSGPYGRPGPGQAAVVPGGTPNGAALAEWRIAAVNGAPMTPVGVPSSPALGSGLGVIPPGSGPGPRALGSLWQHVATRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRX5</accession>
    <name>A0ABQ5RRX5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002421</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="362"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="425"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="483"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="373"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="484"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="608" mass="63351" checksum="B46F623B392EE40C" modified="2025-10-08" version="1" fragment="single">NNNNGGAAAAAGPYGAMQQQQQQHAGNQHHHHGGGGGGGRGSAAPLAARVVQLQLGPLSPHMCYLNLALVPMVTQLAMVAPEPGPNGEEVLQPPQPPADTRVAPVLLDVRGAGYTQLKSLAITGYVDQDCKPATWMSRAWLSRIPAGMPSLEYLALYDTLPSAANDPTAIEGLRSLAHMEHLELRPLYLRFSGEVPCPLSVPPPALPPKLLQLTLKKVVIPVSSPPPRGAAPDSPAALALLPDLPMLTHLWLFDCTAPDLMVFRRATRLEDLRMGGTTTMTRLSDLLPCFPCLRGVILDCSYSVAPGWWSAKQMSALLSLRCLRSLQIDAVSLEPPRAGASSRSILLHGSAAAAKLRGPAATSGLAVPRVPDSDDDSDEYMSTEEDTPMPLGDGMAGPSSSAATLNAAAAAGAFQANIDIAGTQQASGAGMESTEGGPRASEDRGAAAGGSEGSDLASVLPVLSGLTQLTDLTLSFDAPTLFYDENENDEDGENDSNEGGEEGDSDDGSARAGESGAAGPSTSGVASGRGAKGADDWDSVSAGVRDHSDRVFDALLSVQAFVALTALRGLWRLRLGLPHGTVWKDVTSRLLPMLKIALDKTCVEMDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS41</accession>
    <name>A0ABQ5RS41_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein bL9c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">CL9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.430.100">
        <property type="entry name" value="Ribosomal protein L9, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="entry name" value="Ribosomal protein L9, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_bL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036791">
        <property type="entry name" value="Ribosomal_bL9_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_bL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_bL9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55653">
        <property type="entry name" value="Ribosomal protein L9 C-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein L9" evidence="6">
        <location>
            <begin position="92"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="260" mass="28170" checksum="91CC668B4ECDD2A7" modified="2025-10-08" version="1">MQTLRSWALAQQCVRSLLGAARDAGPLVAVESYVPSSNSFERTAGVASSSGLSANIGTSNSQSSFATLAAKGAKGASNERRRSSEEDARLIRVVLRKDLKGLGRQGDVVQVKRGRMRHELYPSGDAAYATPENIAKYALTDAEKVSRDANTQEGVLEKLLRALSRKSVVLTRLASNTDPERFVGPTVSPESIRKAVLRQLGIQLDPSHFLTEADIKTYGTFKVPLNLRLPDDQQIELTVHVDRRNKEVQGASAVAASRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSG7</accession>
    <name>A0ABQ5RSG7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045080131" evidence="2">
        <location>
            <begin position="20"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="189" mass="19433" checksum="8C8B8FA4284E43FC" modified="2025-10-08" version="1">MKALLFILLIAACLGGVLARPATAAKLRGNIIPVTAAGINMDAEDAENHKVHRVVARRAAGETHIAATTAASATTTATATAAAAVALPYPCVQLRRRLRSGLDAGVSLLPCNDDYPIPKRPPPPAPKRPPPPPSPKPKAPLPPPPPPPAPKPPPPPVKKPPPPPRFVNPDPCGLNSAAVGGKRLPLPCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSX3</accession>
    <name>A0ABQ5RSX3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Gag-Pol polyprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">VaNZ11_002897</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The aspartyl protease (PR) mediates the proteolytic cleavages of the Gag and Gag-Pol polyproteins after assembly of the VLP.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0229">DNA integration</keyword>
    <keyword evidence="16" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="14" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-1188">Viral release from host cell</keyword>
    <keyword evidence="15" id="KW-0917">Virion maturation</keyword>
    <keyword evidence="18" id="KW-0862">Zinc</keyword>
    <keyword evidence="18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="20">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="21">
        <location>
            <begin position="516"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="184"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="245"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="766"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="248"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="771"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="GLI60693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1370" mass="151583" checksum="764E63C4AF865A0C" modified="2025-10-08" version="1">MGKWKGDSLKDIKDYIRWSRRAKLHLKEEKLWSENTDRPISAGDKAEDAKTALCTMVGDDFLKEIEEATTAAAGWDLLKTMFVKQMSLHQQEYNAKLSTIKKRDTESIDDYLRRASEYVEILKATGFDVPDSIIVSNIMMGLPPAYDMTCAILSAAAKFHEATVKTIAPALKATELKILGKDEEQGTPALALGNRQGSGGGHRQQQQPRQQQSRQQQSRDDGGSTPTCWKCGGVGHIKKECPQYLAGQQGGGGRSNGGNRGNGGNRGNGGNRGNGNSGGGGNRSNDGRRNAFLLTLDVAKDNSAVNKLRHHIESKGQWLLDSGAGRHCTDCIEDLTDYVEVNDGPVMVGAFGVEQRALGYGRVLFVSTVYGEKYDITVENVWYVPGLGHRLLSLGRLDEKGCRVDIAEGGVAVSSKKNNDRHYTIIMMGYRAEGENAVYHMNLWTATHRERVMSVAVLEESVDLWHQRFAHLGHDGLSGLVKEEMTEGLPLSAAQVEKAIEGKRTCEPCIRAKQTRASHPPSGSTCTSILQRVHSDVCEMPVKSLGGARYVVTLLDEYSSFSLVRPVASEDMVPGVVKEALTLMETQTGQRVRELQSDRGREFIDAHMHGFFGEKGILHNATVPYTPEQNGRAEHLNRTLLEKVRAMLQDPGLGQDFWAEAMATACAVHNCSPVSGSNATPHKLFHGFKPDVSVLQVFGCVAWAYTERKFRNKLDAVSTKGIFVGYERGGKGYRVYLPGKRRVVFTCNVRFDECVLGKSALLPGLSKEAHTDEDDNDENEENPAVDSGELSGDGDDERHAEEIKEAQAATAGEIINPAAALRRSGRDRKSVVKYDPSYIPCYPERVTTATINTLIEPSSYEEAIKTPQAAHWQQAMEDEISSLHQQGTWRLERPPPGANVIPGKWVYKIKRDADGQIERFKARFVAKGFRQIAGVGFEEVFAPTGAHATVRAFLASAAAADELEVHHLDVRTAFLHGDLEEVIYCEQPPGFVTGEPGQKCRLLKALYGLKQAGRAWHRKLTDTLTASGFKVSDADPSMFIKRMGKHVIRILAHVDDLMIASDCIKLMASEKRQLLEQFDLRDLGAVSVFLGMEISRNRHTATLKISQKRYALDIVERFGMADANPEPIPMQPNLVQLTATGVGNVDQDPFEDATKYGALVGSLMYLAVISRPDIAQSVYRLARYMSKPVQAHWEAAKSVLRYVKGTAAYGIAYGRSDVLHGYADADWANDRDNRRSTTGYVFLLNGGAISWKSRLQSTTAASTVESEYMSASFATREAMWLRKLMADLGQSTEAVRIFDDSLGVISLIRNPIKSDWSKHIDVMHHFLREKENHGYVVFEYCRTEDMVTDMLTKPLPQAEFEKFRLCMGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTC3</accession>
    <name>A0ABQ5RTC3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003069</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47435">
        <property type="entry name" value="KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47435:SF4">
        <property type="entry name" value="KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="367" mass="38967" checksum="9CDCE9E60C70ECB8" modified="2025-10-08" version="1" fragment="single">GLFVSHTINILAIMRWTSLPQVGTLPVERSSHSITALGNRILLFGGEHDPRVPVGSELYEYSFADGTWCVVDAKGEPPTPRVAHASAAVGNKLYIFGGRSGLAMGEGASNDLYVFDSDTCTWARLDGQGDIPPKRSYHTMTAVANKVYVFGGCGEEGRLSDLYEYDTTTNTWRQLALPLPQAVPGRGGSCLVAAPKPGGPEGEWVLYVIAGFCGRELDDMHVYSISDNVWCGSNCPSCSPPSSETTEGDGHSHCKLSARSVFGAAVHSCDQGDCTSKDTVVAFGGEVDPSAKGHDGAGDFCGSLMAFGSRGIRDWRAIEAGGDAPGPRGWFASTTTPDGRLVIHGGLDANNERLRDMFVLDMHADST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTQ3</accession>
    <name>A0ABQ5RTQ3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002885</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039902">
        <property type="entry name" value="CCDC148/CCDC112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21549:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21549">
        <property type="entry name" value="MUTATED IN BLADDER CANCER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="168"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="357"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="203"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="527" mass="58544" checksum="37C13DDD6A2FE6C8" modified="2025-10-08" version="1">MAKTQVCHDDIPVLDIAPDQAKREACQWLSTYRHLRTRIQAITSELGSGPACEALKGKDMAAAAFASSAGALQRLESEVWGQVTVARQSVRRITDSLARARPGQSATVDELAEAIDAADRTLTATREQQVAALEQLYHQEAQLEVDIEAIGSQLDADLETDVKRAAALASERDPTVAGPASKRTASAGLRRPTSRGQQRRASTGGGGGGFGPRSGGGALAPEVEEYEEFMAQHGPTGGWDAEDHQEFLAILRSCDGDYSHAVAIAQERAIGYSRREVMDHARWHMEMVELEARKRVAVERWRREKQQQRAALAAQQAVLHSDTAERAQRERQRDQRSREEESLLAGAKKAMAARWRTEREERQREEAEMARRAAEATAAARRNELEQRQAVNKLRLQMCKEAKERQRREEQRRAAAEVAVKAALSAPTPQQLQRVAERSAATFQRRQSLLAKHEALRGRREQVQQHLLEKVQVDVPTDPARLLKGTAAHIQRVQALQAQERKARDSGFILHVSSRVTPTWRVGLQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU04</accession>
    <name>A0ABQ5RU04_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF444">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="40"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="268" mass="30716" checksum="54B6479E2D02938D" modified="2025-10-08" version="1" fragment="single">MRPVDHTIPLVPGAQPTARPVYRLSPLELDEVKRQVTDLLAKGMIRPSTSPYSAPILFVGKKDGTLRMCIDYRGLNANTVKNRYPLPHVDDLLDKLKGSAYFSSIDLQQGYNQIRIAASDIPKTAFRTPFGHFEYTVLLFGLTNAPATFQAVMDRMFRPFIDRFVVCYLDDILVYSKTREEHLEHLRLVLDVMRREQLFAKRAKCFWAQPQVEYLGHIVSATGVKMDPRKVAVVHDWPAPQNLTELRKFLGLTNYFRKFIEKCSIIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV90</accession>
    <name>A0ABQ5RV90_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003427</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016833">
        <property type="entry name" value="Put_Na-Bile_cotransptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640:SF14">
        <property type="entry name" value="SODIUM BILE ACID SYMPORTER FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 10 MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13593">
        <property type="entry name" value="SBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="538" mass="55859" checksum="7A3E7B2538C971DA" modified="2025-10-08" version="1">MESDKQKATKSRWVARWDATRAFIVKNYLPIAFLVALGLALAWPVPGRFLANVSILGNVRICQVAAMVLVFLITGLQLNTSEVKRALALRNLPVLVYGFAAILIITPCLGFALRAAPLNPWEFATGLAIFTVAPTTLGVGVALTEACGGNRAMALLLTVGTNALAVFTMPPELRLLLPHKAASAVGVAADNPAALNMNVQITDLLVKLAITVLVPFIIGKVVREVWPAAEKFAKNHRVPLSLTSTTALAFVVWQTLSAARDLLLDQRPGPVFAMIGLAIAMHLAYLLGNYLVVWFVLRAPLREAIAVVIMSSQKSAPVVVTTITFMTKDAAQQGLLSLPSIVGQLCQIFIGAALAKWLSTVVEQSEQKEKAAAAAEAAAAGPGAVDDAQGGSAIGKTAPAAASAAGVLQLVVPVSPATAVAATCDILGEGRDTSKRAGALSESSAAVPGCLLDGDGDQTYRAQGRPQGGLSGEGTASTLAVRESALLEMQLPSGSLAVGCGGGSLPPVEALPMPKTPKSQIQTSPYQHIPGQTCRPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVA6</accession>
    <name>A0ABQ5RVA6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="38390" checksum="9EB42C7EB3B2B7DD" modified="2025-10-08" version="1">MDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTDKTPGHSIADATRVYVRDAKKYLQAVHGGDNDGIRCLFVGNTLEGAAKDWYRNWTNARGSYTFDELVAALLARFATEVQARDVEAHNVLAAGSYHMRRDENVPAYQSRFEALVTPIADLTESERVFWFRRGLTKLLAKWCATDLVGAQIKSYDVLVAFAKGAEIRLLAGEEEVKSAPRANAVQVQDPLGEAVALDQDLGTTRPTPTAATRRSNGQRKARGGASSGVRGTGGAGPSSDPARNRKRGGAPSSAAAQRSDPNVKRTRTDDTFMPKWNCNRSEFERRKLANVCLRCAESHSTKDCPLMPPFVPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVB8</accession>
    <name>A0ABQ5RVB8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TPPC8 first Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058541">
        <property type="entry name" value="Ig_TPPC8_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024420">
        <property type="entry name" value="TRAPP_III_complex_Trs85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975">
        <property type="entry name" value="TRANSPORT PROTEIN TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24545">
        <property type="entry name" value="Ig_TPPC8_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12739">
        <property type="entry name" value="TRAPPC-Trs85"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPPC8 first Ig-like" evidence="2">
        <location>
            <begin position="718"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="760"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1148"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1206"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1372"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1105"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1148"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1385"/>
            <end position="1396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1750" mass="184399" checksum="805D78BC9701E5F5" modified="2025-10-08" version="1">MDIRPWLHELYAPSILVAATPAADAVLQVKNGLSLVDLLRPYSNVYQLNVPVRVGEASVRVQDLRIRFYDAATLYQPPPEAIEDQLQEVLKAAAQRHARLEPVPDLLQRFMSDNPPDMRDLTPWFTAYRSEFLRLLRFGDHETLDYPVACVYAIPADVVNPASHFDTLVAQLVMPPLMQQDIMYTVRDKDFPRHYLLLLDMAAGMSEKRSVSSLVSAFPGCFSSTIADRALENLAAIRRLYGEAHCSLLRVNSGNGDRAHSGAAVDFFARYQHTCLPSGGAGEPFARPPTPSGGVGAYLTVQDLDAVGAFVRDFVSRTLLPKLEERIGRLNASITATRRGLRNRLNRFWKGTAEDRQTDRPYPWHSVEGQMRQLGDLAFLMRQYAFAESTYRLAAQDYLAEGNSKWYAGVEEMIGLCTVLGEPADPIAAVLLQNDPMKYFMRAHENYIKPPAAVLAAATVAVTSTNGLGGPTAAVPSPPASAVAASKIGRMLATRVILLGTAVQTAAGRHRATSDALMRAQSEEENARAGLLLEQSAYSMLSVHPPLVRKFAFQMVLAGIRYICCGQKRIAIHAYRQVLALYLGKKWKYIEEHLHAVLGVQCVEYGDNERALWHFCSLLDCAHRPAAQQHNNVGQFLNLLSGIRDQLAITQDGANLVDRLPLPVINRHDVRVLSSDAACYAGPSARTVPDSTWVKMEACCTSGSNNWLVDGGTKQERDHEEYSMCVCGEEVGVQVEFRNPLAVKLKVTNVRLICSFTPEGDAGTSESRTAAPGDAGQQAATAKSGGAVSSVVLMDSAHPLLPGQSPAALTSQPGSEFITISEAQLTLHPGEAMVEVLRVLPHAPGWLRITGVTWTLNGVAHGRITFDVKGRRRKRPKGTRPGQLKHYPPHRRLLFQVVPAMPRLELISDPLPAALYSGELCRVMMRIRNSGGLPLRSLALVVGHPEAVCPATDASKDKPLMESLTDPREVVQLSKVPPSLRGSLQLYRLWPGRELAPGEVLEWPLWLHPRGIGTLKLPMVWYGEPAVSGAKGSMRYRTLRVSGSVSVQPLLAARPTVWPSPTHLSHYLLRLAIDNSKEGERLVLQQAAAVTVPPLIGGTAGGVTTGNPKSAGSASGVRTPSTMPLTSSSYGSLSNGWCISLVGAPSSSSAGGAAEQPQPPAVGTASTGSGDGVGGTTVVALPRSLPLAVPLQPGESASLFLQLIAPPPQPPPPQNQMGSAPGTGFATADTVLGRMAGTTGKGPSPLASGMVTPAGSEDPSTTATAVAQVVEAVRSLALADSNATGPSGRLPYVDGSDTGLQQLNLFGPGVLAHFYRRSRRGMTATIGSGASAPAGPGLHSTGLPHRGVVAPPPGPQPSLAQVGAAAVSHLPSGVGAGPGPHGLMQQAPHPQQHPPQLQSNVSFPRGPTYGGPVGVVAPGGLNPAQGGPQGALLPGTASMRALGPAAGTERTAATLTPAVAPGGSGPGRPGQVVPLADPPLDLLLLWHVASSTRTGPHQGQQRIGLCRPYEPQAGRDLSGCLQAQTTPIRMLLTGPNGASSAASSAGSSGPVVRHDFRRSTLAVVPLRLMVRNNMGVEARLQVEVGDGWQSSDALHSWQASTSILGSSPGANLGTTASLSHGGSSSVGVPGTAYAGPTGGSLPAGLASAGPTGGNGAAPGGLASAISMGGGVMSITYQAGLPPCAEFCWVGPTSVAVPRVKPGGEVEIPLAVSVFRPGTYSITGYRCTAYMESSGAVEVQRGEPLCFRVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVK8</accession>
    <name>A0ABQ5RVK8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="156" mass="17185" checksum="2657EA72EFAADF3C" modified="2025-10-08" version="1" fragment="single">MHLCSGSRLKAVRHPSPYQPLQCRLELVEQLSIHQALVHWSCQKALRHSSAGWSWWNSSAYTRPWCTGVAKRHYATPVPVGAGGTAQHTPGLGALELPKGTTKSGQNDEPPGTQDGRTSQRQKQPAHGEDALMMVLAANAARIHQKEKEIKAARRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVN3</accession>
    <name>A0ABQ5RVN3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08056">
        <property type="entry name" value="MPN_PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13838">
        <property type="entry name" value="RNase_H_like_Prp8_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.40">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012984">
        <property type="entry name" value="PROCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027652">
        <property type="entry name" value="PRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021983">
        <property type="entry name" value="PRP8_domainIV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043173">
        <property type="entry name" value="Prp8_domainIV_fingers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043172">
        <property type="entry name" value="Prp8_domainIV_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019581">
        <property type="entry name" value="Prp8_U5-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042516">
        <property type="entry name" value="Prp8_U5-snRNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019580">
        <property type="entry name" value="Prp8_U6-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019582">
        <property type="entry name" value="RRM_spliceosomal_PrP8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140:SF0">
        <property type="entry name" value="PRE-MRNA-PROCESSING-SPLICING FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08084">
        <property type="entry name" value="PROCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12134">
        <property type="entry name" value="PRP8_domainIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10598">
        <property type="entry name" value="RRM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10597">
        <property type="entry name" value="U5_2-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10596">
        <property type="entry name" value="U6-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="1">
        <location>
            <begin position="1205"/>
            <end position="1336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1436" mass="166366" checksum="5C01B4021C0648BD" modified="2025-10-08" version="1">MDLYSHLIPVYEIEPLEKITDCYLDQYLWFEADNRHLFPNWVKPADTEPPPLLVYKWCHGINNVTNVWDTNNGECVVMMQSEFEKMYDKIDLTLMNRLLRLIVDHNIADYITAKNNVVIAYKDMSHTNSYGLIRGLQFASFIIQYYGLILDLLLLGLTRASEIAGPPQCPNEFLTFRDVKTETRHPIRLYSRYMNRVHILFRFTAEEAKDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDMKNRLPRSLTTFEWDNSFVSVYSRDNPNLLFSMAGFEVRILPKIRMAAESFANKDGVWALQNVHTKERTAQAFLRVDDEGLKAFENRVRQVLMSSGSTTFTKIANKWNTALIGLMTYYREAVLHTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYSPKEVGGLGMLSMGHILIPQSDLRYSQQTDLGVTHFRAGMSHEEDQLIPNLYRYIQPWESEFMDSERVWSEYALKKEEAKAQNRRLTLDDLDDSWDRGIPRINTLFQKDRHTLAYDRGWRVRQEMKQFQITKMNPFWWTHQKHDGKLWNLNNYRTDVIQSLGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKYKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESVVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFAAYKWNMSKPSLMADTNDVYDQKPSNKYWVDVQLRWGDYDSHDIERYTRAKFLDYTTDNMSIYPSPTGVMIGIDLAYNLHSAYGNWFPGIKPLVIQAMAKIMKSNPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQTVWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLSQLAKWKTAEEVAALVRSLPVEEQPKRIIVTRKGMLDPLEVHLLDFPNIVITGSELQLPFQAAIKLEKFGDLILKATEPQMVLFNIYDDWLKTISSYTAFSRLILILRALHVNPEKARMILRPDKSIVTQPHHVWPSLTDEQWIKVEVALKDLILADYGKKNNVNTQALTQSEIRDIILGAEITPPSQQRQQIAEIEKQAREGGHMTAVTTKTTNVHGDDLIVTTTSPYEQAAFGSKTEWRVRAISAANLHLRVNHIYVNSDDIRETGYTYVLPKNLLKKFICIADLRTQIAGYMYGVSPPDNSQVKEVRCIVMAPQWGNHQIVNLPANLPEHDYLKDLEPLGWIHTQPNELPQMAPQDVISHAKMLEAHRSWDGERCVLVTVSFTPGSCSLTAYKLTPGGYEWGRSSKDVSANPQGYKPDFYEKVQLLLSDRFMGFYMVPDTGSWNYNFMGVKHSPSMKYGLKLANPKEFYHEIHRPTHFLEFSTLEDADPGVDVENHFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVW6</accession>
    <name>A0ABQ5RVW6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004242</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF94">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="758"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="760"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="865" mass="97165" checksum="6DFE6C022F03887A" modified="2025-10-08" version="1">MQTARGSYHNLAGLVDGGLPHVVQPSPLSGVRPPLSPRPSIESHDGRATRLIIVSNHLPLRVKRGATGWEFEWDEDALVGQAKEGVPKDLEVLYAGSLPVDVALEEQDAVAVQLKRLYNCCPVFLDKDIRDKFYKGCCKQQLWPLFHYVLPVSPDSSGRFDQEMWQAYVKANKVFCEKVVEESATDTDYVWIHDYHLLVLPSLLRKRFNRIRCGLFLHSPFPSSEIFRTFPKREELLRSLLNADLIGFHTFDYARHFLSCCTRMLGLEHETSRGSITIDYYGRTVGIKIMPTGVNPRRYLDGFSWDEFKWRRGELLAQYAGLKVLVGCDDLDVFKGVELKLLAFERLLEQHPEWRGKLVLVQITNPPRSTGRDITELHRCVNNLVDSINRKYGKGSYQPVQYLERHVPLHERMAFYSIADCAVVTATRDGMNLVPYEYVVCRQGPEGWDSGTGPGGQRESMLVVSEFVGCSPSLSGAIRVNPWSVESTADGIYAAIKLTRDHRQLRHDVHWRYVSQHTVAYWATSFVAELQRMTKNHVTMKCYGLGLGLDTFRMVALDANFRRLDDRHVASTYLSSHFRAFFLDYDGTLTGGSSSSLTLAPSEQLLAVLRALAADPRNRTFLFSSSPKSDLATWFASIPNLGLVAENGFFFRAIGSSNWETLVPHADFSWKRMAEPILKQYVESTDGSSVETKESSLVWHYRDADPDFGAWQAKELLDHLEGVLSNEPVEIVRGQGYVEIKPQGVSKGRALERLLASACSSPPSTSSPSSLQHHHYARPPTGLSQTANGLGGPDFMLCIGDDRSDEDMYTSIETMKKSSSTMVFACTVGQKPSRAPFYLNDPGEVLQLLARLVDISLPQHMGSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW45</accession>
    <name>A0ABQ5RW45_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_003630</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="9">
        <location>
            <begin position="10"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="152"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="341"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="38"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="552"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="556"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="577"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="596"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="606"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI61281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="616" mass="66591" checksum="FE505766E3B62059" modified="2025-10-08" version="1">MSGVQRVDDPSLPAPWQALYDPASRLKYYWNPTTNVTTYDRPGGGAAAAPAAPSGGDYFSNRDRREDHGSGANGNGFTSTSYQKTGMSGQPVSTDGFVSAPEYRRQHDISVQGDHVPDPLQTFESVGFPADILDEIRRAGFKAPTPIQAQAWPIALSGRDLVAIAKTGSGKTCGFLLPGMLHIQQTRKDPRSGPTLLVLAPTRELAVQIKTEADKFGRSSGIRNTCVYGGAPKGPQLRDLQYGVQIIIATPGRLNDFLEAGQVRLQQVSYLVLDEADRMLDMGFEPQIQRIVRTLPRQRQTLFFSATWPREVKHIAAQFVVNQTVHVFIGGVEEKLVANKSITQHVLVLNSSHEKFGELARIIRSKPPGTRIIIFCTTKRMCDQLSYNMSREFRAAAIHGDKKQSERDYVLQAFKDGRTPILVATDVAARGLDIPNVAAVVNFDFPTGTEDYIHRIGRTGRAGATGEAYTFMTGEDAKHARDLIQVMREAQQVVPPALEQLAMRGGFGGGGRNRWASGPSYGGGGFGGGGGYGGGGFGGNYGGGFGGGSHAAAGHGAAGTAGGAGGAYGSRDDRDRRRSRSRSRDRSRRRSRSRSRSRDRGDRGRGSPSYGRYSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWV8</accession>
    <name>A0ABQ5RWV8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="122" mass="13313" checksum="7C365F0A32A7A887" modified="2025-10-08" version="1">MGDAAANVVATATMGVSSILMPKVVFKGDNWNEFRRDVENCFLYNGWASALTSPDDPATFKALIVLRRQVAQEYQIELSDCENAKVAWDHLKNIGCDLTQQHQPSDPSAAAPAATTPTRSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX05</accession>
    <name>A0ABQ5RX05_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MalT-like TPR region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004294</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046259705" description="MalT-like TPR region domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="57091" checksum="FC87F254782F4908" modified="2025-10-08" version="1">MSRRGVAMAWPLLKNLALDGAYLCSSYTLTATQAVCAVQATVLARDTIRSRTSTNPVPSLRPSCCRPAASHSPGTSTHVLNGYFNNSTFNWLLRSQYSSAGASWRPQAHARGFSASTAMSYEGAAGGDGNDNRPGTGSGGSGSGPPAGETRISLGEALNQLVDAAIEKVEAGQMEEAVSLLKDGLNTFEPVFTNSPELGELHNQAALLLLFQGRPEEAAHHAQASLDTTKRHFGTRSLLTAHRLLRLGVCRFAQGNMADAAGLLREALDLLDGASTLGAGSAAAGDAASPASDPGSRCEAGFYLDSVALAHVKDGGGVEALSASLRENLRGMKEAYGAESMILSLALSLHSRLVNGGLEQADFLVGEALLKQQIMLLSEVSGPHSEDAAVARYKLATYYYSNDMLQDAGSVVRQAAVALRAHYPEEHDLLVLCKHRLGMICAAQGDHRSASQLLQASRQHFSGAGGQSMLAKEADIGLAMAKFRALDTAREPSARCKQLRHEALELLRTEIEALATAIGHGHMLVQGATRYYGQLSKIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXG8</accession>
    <name>A0ABQ5RXG8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAP" evidence="1">
        <location>
            <begin position="83"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="198" mass="22169" checksum="FBA5E7023316FD43" modified="2025-10-08" version="1" fragment="single">PQDLVPGGSQVVALLPRDVREVGRANFMELAKDPYVSDFQAYVFNALDSMRLSPSLETKTSDQLFSLDVAVSLGGDTGEAVRVAVEANGPQHYLRTHPTALEGSTLLRSRLLRARGWAPLHVHWREWQNLGGDVLRQRRYLRAKLRELMYQEQQLQQQALRRHGERLEEQQQAAGAGPRLELMLLDSTDCADVGEAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYE3</accession>
    <name>A0ABQ5RYE3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS17c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_005310</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_B">
        <property type="entry name" value="Ribosomal_uS17_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019984">
        <property type="entry name" value="Ribosomal_uS17_bact/chlr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004123">
        <property type="entry name" value="PRK05610.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="108" mass="11920" checksum="DC653ABF7060AB38" modified="2025-10-08" version="1">MQSTVLRTPAFSGVRPAVCSRRGAVSVHAVQQVKGVVVSTKMNKTVIVEAERLATDSVYFKRKKVTKRYNAHDESGTLSVGDFVRLDGTRPLSKTKRFVVAEVLRKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYJ9</accession>
    <name>A0ABQ5RYJ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF3">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_2G14390)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="40"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="217" mass="23878" checksum="BB479D6408609E01" modified="2025-10-08" version="1">MEPEFWNNRYNEEEYVYGREPNVHVRQAAERYLNASPCHVVELACGEGRNVAYLVAAGHRVTAVDFSQAGLDKTLRLAAARAGSSAAARVTPVKADVLTWRPTGGPGSIDCVVLSFCHVPSADRPAFMAGIVEMLRPGGLLIIEVFHPNQIHKGYRPKSGGPHDPDMMVTLKELRNQLGHYAGTELLGKELEYVLEEGKLHDGMGAVTRYIWRNAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ01</accession>
    <name>A0ABQ5RZ01_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005402</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20133" checksum="88C6A918F569D499" modified="2025-10-08" version="1" fragment="single">QIGLNGRTLKALVDTGASDDFISVAEVNALGLTPESSDCPQVTLADGGKHPIVGRITLSLSIGPLRVVTRPYVLQGLAETAPYIMGSSTLRQYRARIDMETATLHMCKGTLSIKVPFLSFEHVTPKEGEAEAVPMVNFAVTAMAVRIEPELVGRKEAVRLLRKGAHGLLLRLRVGCCATTAQNSLNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ63</accession>
    <name>A0ABQ5RZ63_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009538">
        <property type="term" value="C:photosystem I reaction center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1470.10">
        <property type="entry name" value="Photosystem I PsaD, reaction center subunit II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003685">
        <property type="entry name" value="PsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036579">
        <property type="entry name" value="PsaD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31982:SF5">
        <property type="entry name" value="PHOTOSYSTEM I REACTION CENTER SUBUNIT II, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31982">
        <property type="entry name" value="PHOTOSYSTEM I REACTION CENTER SUBUNIT II-1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02531">
        <property type="entry name" value="PsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64234">
        <property type="entry name" value="Photosystem I subunit PsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="193" mass="21239" checksum="CF3E2D9060C5F2E2" modified="2026-01-28" version="1">MAVMMRTQAPVASRVSASRVLVAARPARRCVVVRAEEAATAAPVAEKPVWTPPTLNPDTPSPIFGGSTGGLLRKAQQEEFYVITWESKKEQIFEMPTGGAAIMRQGPNLLKFAKKEQCLALTTQLRTKFKLTPAFYRVFPDGKVQYLHPADGVYPEKVNAGRVGVNQNMRRIGQNVNPIKVKFSGRMISPSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZJ8</accession>
    <name>A0ABQ5RZJ8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="422" mass="46059" checksum="10D7A65905140FF5" modified="2025-10-08" version="1" fragment="single">GVLHQYQYTTQQPPQQLPQQQLQQGPQRQCRQRQAPSAAAALETSGPVGLLPQWEVAASSGGDPMAPPNSPAFMQVRVVRAPTPSAPQQNCMAPLPQQQQHQQQPLPQPEAQPQQPLPQQQQQQQQQQRQQQQQEQRQQEEMHRRQSYTGHQIAPQQQQRYQQGHECMLPPGRGGSGGGGGRNHGSVGPTGLQGGNLTTWIPQGYGNSAVVPHPNEPAATTDTTQPVVAAPQAQHQNAPSYRTRSRMAVQPPNVRQRETGAAAPAAENGKKSPHTGSNMLRQLQRQLQQHVQRQQQQQQQQKVDMHLPLQFQQQLQSQHQQQLQPPPNNLNHLLTKRNSVPAAAEGPRGDGEETKRRRGGRRGKNPTPTDTPGRSFGSPRVEVQNGTIATSGSFGGYGGFNGGNISSYTPTAHSPEGGHAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZU5</accession>
    <name>A0ABQ5RZU5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_005225</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14013">
        <property type="entry name" value="STKc_SNT7_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46699">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE STN8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46699:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE STN7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="137"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="599"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="680"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="703"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="767" mass="81676" checksum="5A8726AB0826DBC2" modified="2025-10-08" version="1">MLQRNGVGRIERSAGASTSQTSSPAGLWPSGQRQRRHVHRRALPEMLDALSQVAPHLPLEQIAPPCQVMKCGDIVYRSTLDPSLYKNGGFDWRTATLLATVLGYLVLPPGVLPGAIDYYIKAPLKRKESKAIDKNDIILGKRLGTGGFGTVFKGELKEERGGRTPIIIKKAKEFGEAEVWMNERMSRVSGSHCAEFVTAFDEALNVPLPEGAGGRAAGAPAPPTWSGGVLDSGAIWLVWYYEGDNTLMSLMEKREFPYNLEPLLFGRELRAPRGPVRELVTIKETMRQLLTAVAACHANGIVHRDIKPANCIVSTRDKKLKLIDLGAAADLRIGINYVPNEYLLDPRYAPPQQYIMSTQTPKPPPKPVAAFLSPVLWGMERPDRFDMYSCGVTMMQMIFSHLRNDNNLIAFNKRLQELRWDLSSWRKEEEAKLASGATKAALADGLAAGFEALDADDGAGWDLLTRLLSYKPSDRPSASEALAHPWLTSAPGRAASMQRSLTSTVEATVSTAAAATSAALTSAGRTLGKAAALADMEEAILKTQQGALTEAQLMEELGLQEPAPVAPREGSQTIAWWQDRQTELRSRLVERREKLRQQVSNAMSSVRGGGRGSPVRPAGKGAAAAADSAAAAAAAAANKATDGNGRLKVPLILGGGAAAKKPAGAAANGAKPGQAAGKAAAAANGNGNGLKRAASPVAGKPSNGNENGNGDGNSKLFGGSGLSLGRLTPGNGKRSPLSSRDVEEEVEEEAPVSAKEKVFDLLGVFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0I6</accession>
    <name>A0ABQ5S0I6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005932</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47357">
        <property type="entry name" value="COP1-INTERACTIVE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47357:SF1">
        <property type="entry name" value="SPINDLE POLE BODY COMPONENT 110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="684"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2603"/>
            <end position="2626"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="732"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="853"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1187"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1443"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1598"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1683"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2078"/>
            <end position="2133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2383"/>
            <end position="2417"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2711"/>
            <end position="2752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2609"/>
            <end position="2624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2975" mass="319762" checksum="50E6FE0F303BDF40" modified="2025-10-08" version="1">MPTSTRPRPIWATVGRMSSPGLTSSSMARSSPSKEDEKGLRASVATSSPRHFAELGSPGRCGGSGLAALPGCKTDYDIIRFFTARDIIDLINNLQRSAKIAGDEAGSIHGCEQANRVKAKCSKVCGLSNRLIDLYVATIKAKVARILRKLGLGQAGGQDLLYSHLMTLQALVDKAFASFNAVQHAEAARIKQAKLGADNRRENSGAVTWRTPATPAEQKPASRQGSLGSLPQPAQHLDVKNLLLEARKQFLYMAEELVDLKNKIIVTDRTRIHEAVVEAVAEGEVPDPLPKVKGPPDGIAYGFNTRYVSEGGCNVTGLSLRTVTETVVGYPLSPRREASASSTPYSPRTSRLVHATFENPPADDAQSRMNGLEQARTRLLGLRSVSLPGDAVESHSPLSNGSPCMLESSHFLTPRSRRVISQGGDSDYASSDAFSSKQATARNLFRVDALQATGQLSDTNILAGPEGQATCHIKRVSLLQRTSSLATAPEQTCQLSPAALCPRSRISASGNEESTSQLPLEDAAASIRSPCMGEECLPRLELNDLTATVYQTPSPEDYLRGHPELETLRELAFEALRLVERDPTALDSAFMALDISRAQLDEAAARCQPRNASGIDMQAMLRICLTLLGQQLRSQSVRADANANNVVSRLTEQGIPFQQPCSRCGELAVLLDQVLPLLNHASTGDRTATADLGSEKGPSELAADGSQDEVVLLPLAADVDATDTGDMAHEALATVLQRLSTVLDRLRHLEEESAEMEQTLAQERQVRTDLQAEAARLQAEHNERNDALAVTQQLLTASERQLELLRTQFAELNHELGAKSAEIAVLQQQQQDGSKRETAPPSSVMGLFKMNMLNATELELEGLRIEHENLRSKTELQGSELRRLQNEVSGLRAARDKLVEELQEMQLLLQQRDATSVEDDLVLVPKGCGASEAKAAAIAPSRGTVGAAGAGSLTGKDCKSNAAIAANSTLTGGSIRASATYGAGRMAPSTTKSKAKAGKDLCYRIDNASKAIRPPNSTVATMHSVKRCIPPPPGLPQSGPGVHVSGSSLGPSTPQVHFCALARLEEVQVQLAQAEAARQALEYQLQAAQSLIASKDSEIDTANIILREYERRSLSAANPTNKQAAKLYASVNVPLLQLTAGDLGSCPAAGDALAVVSISPAQGALSDGSYSTRATTAHSQIDLIARLEELQTQCEDLICRAADLDTERSQAQLDADERAAELQATKEYIMELEEEVELLRELADKTTQQMQEKERDIVAQEQALLEAREALARARAECNTQVNVVEAQAEQLEEAHAKFQAAQARVLELKPRDEAQSCADADALACLHEEVLHLKTQFAASQAALADANKDLAAARAVVKASEQSATADRHAVEKLRSRASETEATHFELANKLAIAEQAQSSLAAQVGAAAEDRQQLIVQLGTLTQARENLAAQFGIKEATLTEALLRLEQQEKSLRQLEAQVLTDASVREDLQASLAVMKAEHQDLQKQVVNDRTAVRDLHEKLAETEIARLELADKLEKSVAACADLEQQLLLTQLDRRDPKASLTLASEEAIRNHPESSAEANLLRDEAALECSSRKLATSAAGDRASYKADAVDVLKSEVVHAEAKAQALSDQVRAFEQQLKSAQEREAIMQEQMLMFAAVRAAEAEQAVVTLRGALSAVKARLESVRSEAAIQAGKVETLEIYLAHAASERERLELQAATAAAHSQMLEEQLRQAQAENTLLEQELGATRTGIQEQAALAASQAYQLAAAHALNTEQRERFATEVTSMQVQLQAAFASNAELAQKSACQASELAHVAAARNANTQQAAHFASLEAQLVEARAAVECQAKDAATKIEVLEERLGEVEAFSSKQGDLYRAHVEELESKLGSARAAIIEHTTITVTHVEKLESQLAAERLQSIAEAERSSTQVSVLIEQLMAARTEMAQEAHTSAARAVNLEVQLAAARSAVGALVEHSAAEVSSLIAQLDAVRKSGVAEAEKVAQRVTELELHLASAQTAHAALADRSVTKVAELEARLIAALTENIEHAKCSATQAADLEHKLTTAVALASEYTTSQTAQLTAQVVAARTAMSEQAQRASADAAELVKQLEAAHAEIAAQVERSAAQASELEQQLSAARAKNSELLSRSAVESAAWEARISAAQEQHVVQAEAFAAQVAELEERLTTARTGIADQMKRSSVHVEALEEQLVAAHAEYAIQAERSTAQTQALEVQVAAAQAAIAEQTRSLTAHIAVLEAELAVTRAEKFELASSFTLQTGELEVRLTAAQAERNSEAERHAAHITDMEAQLYVSKASTVRHANRAAALATAFEEQIIAVRENSTAQEERIRVLEAELRTVLDTLGTARDRQERHTEDVKQLIVKEQEQNKQLYLRQSEVRQLKDTVAGLQTKLQDREAAVADRELQLENARKELECVVAVLGQLQTTVERLQCAASAVTAAGEAASNAAAAAADQQEVNLAVTAREQAKLRSLLSELEGIAINQSHVVLGLKDEVATLKAARDVDAGQLRDASEQGVIVAMCTEVTQRQQPTVVANFDAPAVRVQEPHLATKELGGDDSCSQLELQNRTGLVVALAMGTEQHPSRALKGKVEVKTSLAAEEQEHTSLQSSADATQRQASAQHAVVTTAERRLAAAVEGAVAEVQARQWVSEAECHVVQDLRAAVEEQACARLQLSCDIKEHSKDLAEELAGVQQLLNRQRRASADLLETLTVKLAAAESDLRGAKAHVEELRSELTHAMRRVAAAELAASTADTEKHQIEVRLAAAVADSNSGRAMVQELTVELARYRKEVDLVHKSMLAKQALLDEHESEQRVPEQAENRNLWQDSMPAADVEVQFGAELVKDFLEGCIWGMGLSSASGPAPMLSGRPLASGTPPQKPIIAHRSGLASTTAVRQPVRSVSDEADIEARALRLRALEGDVLELMSKGWHGNRLGGLEPRTCVESSAGVAPAIGAVTRDAAAPSAPSRRISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0M1</accession>
    <name>A0ABQ5S0M1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 92 protein</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_006329</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 92 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF69">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI63438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="454" mass="50504" checksum="DD909CC70718631F" modified="2025-10-08" version="1" fragment="single">KKCYLAYCFILTEPSARTLLKIATGRDHCCVPVPGFLRRLAGIHHPHVAPASRNSRVSQWFDMATQPQHRGITRRSAQLTPASNTALLAVPLLVLCGLPLPLGPMGLATAQTLLEADPGSLLHSASAADGITGMQGLLGTRSTSTPGTSSDGYSALCVVGRNENPYIKEWVEYHNCLGFSRIYLYDHNSTVPLLGEIKDLVDSGFVVYTNYSGMHTKFKDGYTSDLDRFMSTVQGQAYKDCVDRFGQRHTFLGFIDIDEFLVIYDEKINNVDDLLREYEQHPGLSIYWVLLGSSGHKDRPTGLVVDEYKACTPHDHKFNTQFKTFANTRFRPVMYSPHRAVFAAPPDPETGLEPYMVDENHNRIPRGRNKNCTHRRAAVFHYVTKSLTDFNEKMHRGGGAGVTRPTYYFDLMDKYSKETCEGAVQTRQRFCGGKLPHVLSLVAAGAGSTAINME</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0T3</accession>
    <name>A0ABQ5S0T3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RAVE complex protein Rav1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006472</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057853">
        <property type="entry name" value="Beta-prop_WDR11_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057854">
        <property type="entry name" value="TPR_WDR11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039694">
        <property type="entry name" value="WDR11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14593">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14593:SF5">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23752">
        <property type="entry name" value="Beta-prop_WDR11_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23753">
        <property type="entry name" value="TPR_WDR11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WDR11 second beta-propeller" evidence="2">
        <location>
            <begin position="128"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="WDR11 second beta-propeller" evidence="2">
        <location>
            <begin position="452"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="WDR11 TPR" evidence="3">
        <location>
            <begin position="1218"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="domain" description="WDR11 TPR" evidence="3">
        <location>
            <begin position="1375"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="756"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="893"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1194"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1568"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="392"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1587"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1620" mass="163028" checksum="A1F0214190A19B41" modified="2025-10-08" version="1" fragment="single">EAAAATVPSSRSGAQQEGVQAATSDQDRAPPQLLVLVAFTGDGRVWRWDAPLPALTAAATSAPAAPSVLSADHAPHLHALAGAGGAGTSSSYSGAQLQRPAAGLPERLPAVPRSVAGSPQLTGLLHTLPSAVSTFAVYPRPVAATSLRPGAAGAEAGGGVVLLGAAGTMGGSVELFTVQRGLVSPLALHVSSSLLVHKDAPVRGVRWLGASPRLVTFSSEKGGTHDKAQWNNHLNITDVRSGRTVQLPRWEYGNGPGGGGGEPAPLLGVRASPCGSYLLVLMKGASAELWGVWPGVTPTRLRLLDLPFSCVEWVQPPLPYRGQSVQPDDLTTWTVSSEPPPGPPLQPQPTAPPSVGRGSYDSSGGGAGPGGSYWSMPQRLASGMGERPTDTPSAAEALPPGASSSSSQPPLPLPLQLPPPSPPPAQQPQFLSGQSLPAPQPLTSGGLEEAQLIFTLADGRAGVLSVRGRRVADTRVKRPMAGLLSPLEISATAMAAVGRLVVMGDAEGRLAVWEWSTGRTTLQATGLGLVRKIVISPAIAGTAAARPSLNSPPPPSFSASALSLMLPSESSFLAPLLPAKSASLATDHSPAVADFPPLPPPSSSSPPAHIGSSSGSMKARVAVLFATGHCCVFDLDSTNKLRLVVATAAAGVRPVLDLALLPFWWIDPGTQTVGSGDGSSGNVSLMAVVTDEGSLSVLDLGPGAAALLPTETQQLVPYPAMASPLRPPLPSGLPLGKPHERVFGGIIGRNADGGGGAEAKASLRPSRRLPSAGGGGSRGWALAAALQPPLGPATSAVASGASVEGAVHGALRLHLAQARGLASCLLLPRPWAMLLRLLLQLGVSQELLRYLCGAYSPGEAEEEDVRLELLALFPRAARAPLLELLADRGRLNSQSSSDVHGSGLPPPSHAVAANGSRGNAVPALDGPWIPSQTTATATTATASAAASASGSNSTGAAAASTLLALSNSFSSTALALSSNMLRAVSSSLASTLTPAIGGAAISSAAATVAAADRPAVSGTEGLRVGAEGADPGPATGDVDADADVDGGGGGGIGTSSSRGRLPHHGTLLHLLRMLASMRAQGRLLHPDEWLSYSRALAPGSSPAERMAVAAHVAGDSDEARFWSHLPATLASVRERQEYHIPGNWAEGVDGTAAVPHASVAAGRVGAGSDLPYRQLQRTASASAAVAVQPAAVATSATELPGPSGPESRSGPRTSSVSSMTTTVDMYDAAAAGGAATSVGGGRGAVLLWDERLVLAEAQERIKWREAHSGAVLDMGPEQQERRLLEYMSVGDLQTAVAFLLASSPENSARYYRDALCTLALAAAAAAGAAAAAASTGGAAAAGASGTSAVPPRAIHGGGGAGANGSGNVGMLLAQSAKVISAHAASIGDVHLGVPLHLAAGLHAEAALVLQESGMWRLAAVMTAHFLTGTERAQTLQRWALHVWRQEGSVWRGVGLLAAAGCLREALRLLLDAGLPDCAAAFVAGCKHAGLESALAVPAPAAPVADGATVDEWIATPRVPASAFVNSAAAAGAAAASSEPAVLFDGGLTPFESMGSGIGLGVGGVGLRPVTASRPGTRGGPFSSPEQSNSSNGGRRFSSADLMRAAAEFDLFVTDLLTSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0U7</accession>
    <name>A0ABQ5S0U7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5'-nucleotidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006498</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008380">
        <property type="entry name" value="HAD-SF_hydro_IG_5-nucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02244">
        <property type="entry name" value="HAD-IG-Ncltidse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF15">
        <property type="entry name" value="CYTOSOLIC PURINE 5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05761">
        <property type="entry name" value="5_nucleotid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="66"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="599"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="66"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="462"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="702" mass="77999" checksum="53F0A6828BED3990" modified="2025-10-08" version="1">MFVWTRGHAPANLRAPAMSGQLSGRVWRQARRLAHLQPLWPSSHFSSVSFSESRRGLSLAVASNSTNGAAASSSSSSNRNGSPARSAPSPGSSASGQAATSAAATSAAVTATPTSASREPPQRHISATKGYGSHPPPPRGTLPLWTDFPIPPLQRLPVQRRVFCNRALNMKQIKAIGYDMDYTLAQYKPDTFEGLAHKETVSKLIEVFRYPEELRQLDFQWDYMTKGLIIDKERGNMLKVDRHNYVKVAYHGFKELPADVRKAVYNGHHSSGGAGAGVFDEAGGYAMVDTLFSLAEAYLFMQLVELKDHDRHDILRRKSCKDLYRDLRTAVDMCHRDGSLKRAVAANPEKFIHRDEHLVHVLQTHRASGRSVFLATNSLYDYTNVVMNFLVSGKTGAQKTGEWLQYFDVVMVGCAKPSFFQHRAPLFSVDVNTGMLKNTDNGAPIIPIDELDRSEESAAVVPSPSTRAGSGGSSRSITSPPAAVGVCPVRCGSNVFQGGWYMDLHKMLGVTEGSQVLYVGDHIYGDIVKSKKDIGWRTMLVVPELELELEKLGRSMKMQEELRQLRHLRDEYDDRIQRLRWSVRSNKGMEEGGVEDQAYRDALQLLEAAETDRAQLKARHSALLRNHHRQFHPVWGQLMRTGHQNSRFAHQIERYACLYTSHVNNLAFVSPEKSFMGRMDLMAHEWEDAEAAGPGAGALGET</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S137</accession>
    <name>A0ABQ5S137_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_006611</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019148">
        <property type="entry name" value="Nuclear_protein_DGCR14_ESS-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12940">
        <property type="entry name" value="ES-2 PROTEIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12940:SF0">
        <property type="entry name" value="SPLICING FACTOR ESS-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09751">
        <property type="entry name" value="Es2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="30"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="323"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="399"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="473"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="505" mass="49313" checksum="097B7811DD9C12F5" modified="2025-10-08" version="1" fragment="single">GGPAAPGAELSLDAFLSRFTSEDNASFQAILQEDNRRKRAKVRHHLERPDPRDPETRQLLLEGPKDRPSDEFGSTGQAPMTLLPAPEPVINYLFYDSSQRPKVPHSVKELAELSLGPPKEINKDGTRLKAPPEAPPPSAAAAAAAAAIAAIASGDDAAAAAAAAAAAAGAPIGAPMPGGLVGTVGYAPVRTPALEDVLASPLMTWGDIESTPLRLGMDDMPVAIDLEALMGAGPSFHVQSLTEREQAAHRLAASKAGSGTAAGRAVTSRGSTPLLTALRRATGTGGGAGAIASTGGGSGTPLAVPLSPAARQLAANIKAGLTPRTAAVSSGSGGSRGGYHGHHHRSGSGRGASGGTANGNHAAAAATAAAAGKTADLGHGELDKQLRASYRSAVVASLGRHGGTPGMGMHPRTHTPAAGGSAGRAAAAGVTPSRGGGSGGPSSRPTSTAPGGGGGGGSAPKVVGAGNREVELPTTAAGPAGAGSNTRASSAGGSGGSVTDNLLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1L1</accession>
    <name>A0ABQ5S1L1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIP41-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="40707" checksum="BA7E6A1B0D1753B3" modified="2025-10-08" version="1">MPNSKDTFHNAGWTIRTVTGPIMGDKEMDEYRRVLSAFVLPEMVFAQNRVELVHEASGTVISFNALDALAGWKHEDLPPLQVRVAEEWRKAREYEIKQQKALQLTYDWTYTTPYTCTVARRRGQQQQQQQPALAVTAAPDSIGAHGIPDFVCGADSTAAAAPRKVTVAANIAEATLSSSSDSNAAGVDAGAATEVAPVGGSRPSVSHGTTSDLADGADGGALWQPTTDQIDRGLLMERDPILFYCEVPLYESDLDDNGVCSLAVKLRVMPRCWLVLLRLWIRVDGSLVRLRETRLFCRHDKRLEVLQEVKHCEGTFAELRARGAPDEGPSYIDGDAASQVCVCGGGVVHCVWATRPVARTSTGDPDQGDEQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2B5</accession>
    <name>A0ABQ5S2B5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47556">
        <property type="entry name" value="SEC14P-LIKE PHOSPHATIDYLINOSITOL TRANSFER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47556:SF1">
        <property type="entry name" value="SEC14P-LIKE PHOSPHATIDYLINOSITOL TRANSFER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="123"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="276" mass="31539" checksum="A3C1134A804A20D4" modified="2025-10-08" version="1">MGLRKISQYSVLHSPCSICAAQRLQVVQASRRQLIRTCAVGPQLATSISSAPGLKSEPWTDSPGQQNALVELKQRFHNEAAPPDEATLKWYLRDRYFDVDEAEQKLRTMLSWRKTFQPQSTTPQMVAAELASGKAYVHKYTDRYGRPPIVVRTRLHVTGQYPLSDSKRLAAYLIDTAISQMPPGGEQIVGIFDLREFQFAQNADFQLAAFLVEAFFEYYPRRVSQVLFVDAPWVFFPAWEIIKPLMRKYATLVRFCSVAELRSEFFTGETLPSDFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2S0</accession>
    <name>A0ABQ5S2S0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA 3'-terminal phosphate cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007389</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00875">
        <property type="entry name" value="RNA_Cyclase_Class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016443">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03400">
        <property type="entry name" value="18S_RNA_Rcl1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="5">
        <location>
            <begin position="3"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="6">
        <location>
            <begin position="180"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="40488" checksum="751DAA4C3F0A343F" modified="2025-10-08" version="1">MLKFRGCQHFRQRLVCSTLSGRAIRIDDIRAADPESPGLRDFEASFLRLLEKLTNGCVVEINETGTSLRYRPGVVVCGSGLVHDCGTSRSIGYFLEPLLVLGFFGKKPLSIILRGITNDETDPGVDTFRTVTQPLLRKALGLEDGLELRVVSRGAAPHGGGEVVLKVPMVKQLGPIKMEDEGMVKRIRGVAYSMKVSPQVTNRMVDGARGVLNALLADVYVFTDASSGASSGKSPGYGITLVAETTSGCLISAESCHVARRNEEATDEAQDRGNQQQQVPEGIGCMAAHLLLEEVRRGGVVDGCHQGLLLLLCALGPEEANSVRLGPLTPHAIRTLRHLRDFFGVTFSLRTERSSGTIFATCVGANVRNMSRRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3E5</accession>
    <name>A0ABQ5S3E5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007173</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47357">
        <property type="entry name" value="COP1-INTERACTIVE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47357:SF1">
        <property type="entry name" value="SPINDLE POLE BODY COMPONENT 110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="780"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1189"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1332"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="240"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="541"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="667"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="860"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="413"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="787"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1224"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1343"/>
            <end position="1354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1386" mass="147787" checksum="BF6FFFE8B798EC2F" modified="2025-10-08" version="1">MDSSGPNSYGEAEGRRTSCTNGLETTTLPMRTMTSLQSRSSIVWPPDAKVLSTSEQGSHLPGRSEGVVPLHWGTTRAELEHAFQKPMQQYNVDELTVEWDGLFKGDGSVDLHKPADVYRELRKDLRSLAGVMKQSFRFVVDDRRWPSGGTGPSSGGVLHLEPCLSELRSVAQEIVNATRSASELRIELERLRQANHLLQAQVHEAHVSLMTTEVERNDALSRLAAAELAIAAEAKRAAACEAVRKQNNVLQAQLQEAGGEIKELEEMVRELEERLATSKAEADGLAAQLRAKEGQVISHNLHGRKLSIPSEAGAPEQVDAFLEETHRVAVVSAAHCSKQQRCANASDHHAAGQRSAVGAAPGGDLTESGDAHWMGLANALVKGSRVEVRLREMPPELRRDVQQLLAQFIRKLKEDKDDSKGDDVLNPPTHPPGKHGDVDASVKAAPTAMQGGGHQVAPGIAGAISGTFAASACTVSAGGSEMLHAHVGADVATHHGGDLVDPHLRLPISNTQARGELHLQQQQQQQQQQQPPLLSVHLSLQQDAINELEAAKETIARLTAKLAETQQAHCELQQRLYLADTERAAAEASRSETQATLEEALRQATLAAETAEAEAAAQAALAAEAQVRIGEMQKALQHAVEARNALEVELQDLPVSRQHITFLESQLASCAAVIKELEGRRNDTEQQEVRERQARDAAAKELEAARQRIVRLQADLRESRSDAKIARSEAIAASRDAKAANSGLVKAQREMEEAVQRLRQVESERAALAAALAVAEGGRGQEAQATAREEAAVAEPHAKARGDSEASGNEQHKASGCEGGPPHLTTGPVVEAIDVKLLALELGVAQRELEVAKTRLAEEQSISKQEAARLEAKIQALEADLLATRIEMGAAESAYRERLAAMEAQLAKTEAEAGHKAHIANTSLLEESLAKAQQRVAELEALQRALEQDTAAAVAPASAEAWERLQQLESQLHAAEAALQQAEQRYREEGDSLRMQLAEAKQATAAAMAAAEAAAKQSQGTSVSPVRATDDAKAAVEGQQPEDDDEVDTVVLRARCRALRAELTRVQSALASVMDTWQAAAQDRLGEKLVEVQAAAAAGRVKSDDEFWAHNNEVHSLYALVNALRTSSMYMGAPGTIAAYGDNRADGAGSFGAANCSLLGTSYRRLRYHQYKETLAQMEQLLAGAAEGLLRRPAPPPPPQQQQPGLGGRTDRVQASGRGRESSDGNPSQGRSKGHGQSRNGSGVAADAAGHRANGCHQPGPSLPTGRGVVAGANATSPRRLGRHNAHTGNNLSGGGAAQHVVCAGAADLGSMPTIESSKGTGVGYGMGPSNCTLGRQSAPPLASSSFTSDTGSTRLEDDLANISLLYEIPPMGSPPKYDFSSWTWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4K6</accession>
    <name>A0ABQ5S4K6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008090</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1340">
        <property type="entry name" value="Mediator of RNA polymerase II, submodule Med31 (Soh1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038089">
        <property type="entry name" value="Med31_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="136" mass="15748" checksum="C51427B5EC3EF333" modified="2025-10-08" version="1">MSTGARRVWTDQEVGISGHERFALELEFVQCLANPLYINWLATKQYFDSPSFINYLKYLQYWKQPTYANHIAYPHCLFFLDLLQDPEFRVAIKDFSYAEHIRQAQETFFRNFHSNRVADATGAVQGTAVGTGDPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4R8</accession>
    <name>A0ABQ5S4R8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007886</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF21">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="798"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="83"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="168"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="237"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="330"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1020"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="427"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="447"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1071" mass="113647" checksum="95BF9F845D3850CC" modified="2025-10-08" version="1">MAGIGARTNMQVMQSPNSDACGLWRQPQIVQRLGSSIFRCSFRGKLTPTTSSVAAPERNSSVTSEVPDLLGDRQPCVIIPSTSLIHGSGDSTDQQVEMSTSPPSTSGRTHIVDGQHDRPFLPGSRRRMRNLSSRRNLSIASFYGEYCDMKATQRMVSTIWERLMSQGDSAGTAPASESVVSSDVGSSFQQNEGVVQESPASQQQQQLPAWALPGENGSSSGGGSAVNTASTIDRDFVPTTTPAIFPGATADGPNRSRRTTPALPLADGWSVASAVTTDGDVANAASSTPPRPQPPSPPHKRVVLYLGPDLELQLPPPSFGALPLVGATWSDTGGDPGSSSSVFSEQELRESLNAPKPRRVRGSRLSTSEAVRAVSFDGATATNTAGEQQGVRAPKPRRRKAMKTTMAATATANEAVQHVSKRQLTKGGKEVTDEEVQAHDRGCSPQAVAATAAEPSAITAAPGGSSSLGRQKAGRDPASTANRRRSERPSDSVAAMVTTAGSAPSPRRPEFSYVPQHAARQKDLLEALVERMSDAVVTERIQQLAKVNRAGVTALNFVIQALGEMGHVYALSRLLTLQVALGLYNEHTYGTAFRALHRAGRLGMALAAFQEACRSGRDLGPIACSVLLHIASLERNIKLAWQLFDQMIAHQMTLNRYAYNCMAHIASLHGALDDVRIIYGMMKAAASGNGGASSSASSSGGENDCRPDSYTYSAMVRAAVNAGRGDLLPALFNEMVASQRAEDRAVGRLRPLAPGEGEGRLSKEVWSHFISAACRTGQSELGQRFFNAGITELGLLPNREVYNVLLAGLAKTRPLPELLQLYGEMVQGRLRSGPPRASWAAGANAVHPVDGTAGPAAAAVEEDPPGSVRQAPLTPDHYTFNALLTAAAHHMADLPTLDRIRREMVVHGVGINLFIGTSLINAYRRTPELTRSGAATAASTAVDGVVAAAAAVMSELVRSGVASTMSYVCMTAFYATAQRPVEAVDCVRQMTERGFLADDSAWQFLMATAGDAGQSSLVRIFAAERQRQAAERLARQLQQQKERAERRIGSSCRSAADAVVPTKGIAVSFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S559</accession>
    <name>A0ABQ5S559_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase M43 pregnancy-associated plasma-A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007894</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M43B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47466">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47466:SF1">
        <property type="entry name" value="METALLOPROTEASE MEP1 (AFU_ORTHOLOGUE AFUA_1G07730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047324700" description="Peptidase M43 pregnancy-associated plasma-A domain-containing protein" evidence="3">
        <location>
            <begin position="30"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="727"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="827"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="733"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="830"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="885"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="895" mass="98790" checksum="D1E84894A7AE9F63" modified="2025-10-08" version="1">MPCVAYMHHLLRLLMALVLLVALPAKGHATSNLQADHAMEARMSWLGSISQPFRVIKDPLTADNQAVKRMYEQSSAAAVIAYLLSGAAKSLSAAEGSSSALLLCLSPSVIRVYFSQPKMCSSSLVREMYGLSQKLYGTPVLQACQYTLYTAFKSVFTWVRHHIPLSPQHITGQPNATALWPDSSAISDTNFSAAVLDALLTGQGDLALVSTTVSEQCGLGVSEFAALVPRISSVLKQLHADLPDLQEIVEDRIRVARKMQRPNQRRQALEVLEGQDGHAPQDSNMSMPMSPEVMEGGGPPTDRFLQQVDGDYVLDVPTDISYKMPPEEFQIRPPGEMPKVMVGLIFHILQYKVNKTVNGPPGVEMVGTYVGRMVRIANIMAKATNFSYFVKEIRVNTKAYPYLLLKNRKAWLNVPDPYSCSGGVCLQKSSFVSSTIADFPRSYNVYVASDATVTRGALGFSYITLSDVLPTFAFMFLAWDQLSFDGSNSIGMYEDGTTTLMHETFHGFGLPHTFDYFGQQCVDGDGIQDTPVVYDNMFRTPFLRLAMDYCMDVFWRMYGGDWETVYERWSVLGIPDLDKNAWADSCPDLPGYDELGNFMTYNTPVCFPALGHFTRGQAQYAHYVTSTYNRVMYAWGQYYAAQASYSPPQAMPPAPKMYDLMCKATKTSCPCKSSWSVEGKSYSYCGGEIEPLRCEVQDPRSCAGCKNVVGQCILTCGGTAQQCSTSWSAGAPSAPPPPPFPPSPPPKPPPPPPRALPPSDCTVTVSNCSCRADWSYKSKLYEYCADISLSSTRPHELWCIVSEDCPTFRSNPLQECSPQVNTRYCSALPRPSPVSPPHAPREPPPPANPRQPLPPAPQRPRNPSTPHPVIIRRSPRPPPQPPRPKTPRKPRPPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S574</accession>
    <name>A0ABQ5S574_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI65037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="104" mass="12201" checksum="A2BD3602E4665608" modified="2025-10-08" version="1">MPAHETLHEAPHREIHYGFDDVRHPPCYFFYIQDDRLEWKEDASEDTNGVCSTFCEDGGGHYFDIHVGPFGFGQQVSREVMAEFWRRFGIPKKHVDAVTRGRTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5H0</accession>
    <name>A0ABQ5S5H0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="429" mass="44673" checksum="0F58FF686248F1D2" modified="2025-10-08" version="1" fragment="single">HLAWMPSGNGTASGQVAPPPLMPEDSRESKWRPAFSLHDDSAGDDADCSDAGIARDVPGAVMVMTTAGASERPRSAVALPLASPATFMSLTAVVVVPSPRPPPSSSSSSSSSSSSAAAAAAAAAGPATGLGSQEEGTDSAQRPRDSPGIGYLPLPPPPPAPAVHLRQLAAESETANVTMRMPCAAPGESEIGGEIKPATEPSLSRRFARQLSYLGPVCCSCGVTVMCCKRGYGFNSAAAAELEKAADAACDRREALPPPPPATECSNTAPSAIQQLELYERYGQLFEEMKGRRFCAVTFQVAIVCNAVIPAVLLGAQEGANTEPYSAAARSTIIALLICKVLFAFYMTVTLPYSNVVTMGAELFCAWLEAAVCACLVCLQWIHRSVPSLSDAMLGCEVTVFTLQIVRVALTTVMPTILAIHEEGCNLGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5N6</accession>
    <name>A0ABQ5S5N6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="136" mass="14665" checksum="51C37E71D1C27FB6" modified="2025-10-08" version="1">MASKPGPSAPVQATVQPQQAVVVEVAASSSSVEVISAIVNSASSPPPRTGNPNRWLQIGFFFVIIFGCIVYFREEIYTFTTDLHKYPILSVAEALQVRKYLGPNWTFGTGQDTVTTLDGDKTIIANLTLTTPPVAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S672</accession>
    <name>A0ABQ5S672_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_008953</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23802">
        <property type="entry name" value="UBCc_UBE2Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF39">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="4">
        <location>
            <begin position="770"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="341"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="411"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="998" mass="108353" checksum="5A7321F986732CDB" modified="2025-10-08" version="1">MEVSNTPVPAPADMEDEGTIDKRFGSDDDMDEGSDADWDNVASGSDNGCGGNGDGDQQEVDDSGDDSDEEERDLIILCSMSLGREQLYCRPWRTERCVRVDAVCFGPRWERYEASVEAAMEAETGKQALSSTQGLAARRQIFNPREAFSMLSRELLDCLRARNVDCSVETGGDDLYHWIVDLGSFTEGSALAKDMREVSRRYFYSTVRLRLRFMRGLHPFFPPSVEVVWPHFKGPMLDAVASHPMLRLENWDPWRAAMDTVGQIKAFLEANARVDLDNPANDIRLHPHSTYSPLERGLARLEALSGVSPLSLQQPEVAALYAARDSYDKDTARLQALAEAGKKRGRPELASGKRTREVVWARGTGYGSGQEKNSEQWDAKATDAAQRARDSEIQLLLEELYNSLVRELGPRPPLLEEREDTQRQKPQGQQEEEQRNQQLQEADDGTGPAAMEGVETTAASAGAAQEPPETPEPAGTGGVRNAAVAVTAVEADAPVGAAPACSHGDDTEPRLGAERCAAVLQDSCLLPFVVRELHLASFTDMGARHKYYLALLALLRELCRPSTHDMLWSEVGPQGQASSEPAGPGHSVAAILTKALRSAAKLYLSVLGPATVRAEKGTGSAESGASTSAAVTATRIPDRSAVAAENAAREAEATQRMARIIMQIAAYIDANMPGRGAPAAEVASAVQGPHKYPQPQAEASVATGAALAAERTASGAQASTSAAGAASASADGPSLYVKTFKPLQVDAVSGLGRAHHFANQTRMEQQAPRIRAVRLAKEVASLESLLPLSESSSVFARVDETAVQLWKTLIVGPEDTPYSGGCFVFDMYFPPQYPNVPPQVHLMTTGGGRVRFNPNLYAEGKVCLSLLGTWSGDRGETWNPDISTAVQVLISIQSLIMVPEPYFNEPSYESQTGDRGKTASQEYNKNVRENCVRYAMLDVLRHPPTALAEVVRTHFRLRRDALLTQIKIWADDARPADSAYAARMMDMRRDLETLIAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6P3</accession>
    <name>A0ABQ5S6P3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009055</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family. CGFS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF16">
        <property type="entry name" value="GLUTAREDOXIN-RELATED PROTEIN 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="7">
        <location>
            <begin position="91"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="185" mass="20489" checksum="C5508EF4E6940305" modified="2025-10-08" version="1" fragment="single">VFSPYVDSLLTFKHIIAKQLLSNAYYKMLTRFATSALSGVRRTVSTPAWQLQRYFSTDPDSHDDFKPKYAASPAIGNVDEQIKHSIANDKVHVFMKGTPEQPQCGFSRMACVVLQAYGVKFGATNVLADPAMREGIKKFTSWPTIPQVFVNGEFVGGCDILMGMHDSGELEKLLAPVREAQVPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7C8</accession>
    <name>A0ABQ5S7C8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="12935" checksum="C2BAA0C9FD860F42" modified="2025-10-08" version="1" fragment="single">SAYTAPARFTATECRRTSELGMGASVTVDLWQPGERPQDVIRKWQQRANRWRQRKEEATGYFKGGGDGGSGGGGGSGGGGEGAGGGDMAMMAQMHAAPAEVLSEVLARHGDSIGQALQAYMTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7G8</accession>
    <name>A0ABQ5S7G8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="108" mass="10636" checksum="6704C6BE4F419B29" modified="2025-10-08" version="1" fragment="single">PSPPSPPSPLPPSPPPPSPPSPFPPSPLPPSPPLPPSPEPPSPMPPSPSPPPPPSPFPPSPPPPPPPSPLPPSPPPSPPPSPQPPSPPSPDPPSPSPPRPPSPEPPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7K0</accession>
    <name>A0ABQ5S7K0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM4</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_009191</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="515"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="940"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="946"/>
            <end position="965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI65621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="965" mass="105294" checksum="974D4E085C2D1ECB" modified="2025-10-08" version="1">MEQDAQPLHQEEAPSQSQHYEGNSTPQAARRVSRARTSPGASQQPGLSQQGTPAPPRRPQPQPSGSFMQGGSVLQSEGTLGGGATPLPRGLAQNRGEFGGTAQRRPNVRIGTAGRPARTMSVSQVSGDQQQQDGAAPAEGDELPPVQDDAADDGTYVWGTNINSRHLRQQIDSFIRTFRPAGADPIAQAKYVQIIKEAIDAGETSINIDTADMRAVINGVSYNDLYETLVDFPREVHCVLDEVVREVAVLHLNFEPELENRQQAMDLCLLVCRPYNLAATKHIRDLDPSDIDKLICIKGMVTRTSAIIPNLRYAVFECAACGHEVATPNVNGRVEDPTSCPSCKKKWSLVLQHNKGSYTDKQLIKMQESPNDIPEGETPMGVTLYTYDTLVDVARPGDRVMITGMYRAAPIRANPRQAALHALFRTYVDVIHIHRDETRRLFTQSVGQDGKAVDAEPTILSAPNTPADSQADLPPQSLTDPVGTEDQLVAIENITAEELQEVEGKIKELSRDPRLVERLIASLAPNIWEMEDIKKGVLCLLFGGTPKIFPGGKIRSELNVLLVGDPSVSKSQLLTYVHQLAPRGIYTSGKGSSAVGLTAYVTKDPETKEMVLESGALVLSDRGVCCIDEFDKMSDSARSMLHEAMEQQTVSVAKAGLISTLNARCSVLACANPVGSRYNPQMSIAENINLPPTLLTRFDLIYLVLDRYEEARDRRLARHLVSLFHPGAQSRSRAGSAAGALELVSPDLLKKYIAYARAKCHPKLTDEAAEELVNRYQTLRRDGRERKVVMATPRQLESLIRISESLARIRLDERIRAADVAEAVRLWYGAMAGSAGSSDGRPDLDTLYCGTTAVQRAAARALPEELRTVISGLRMSHTFSVDDLLREINSRMATGQQQQQGGGATAGGVSRSMLITALRQLDDLVLYDETTNTVRPILRSREQNVPQAQKEAAQAANANPPIVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7T1</accession>
    <name>A0ABQ5S7T1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_009181</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04842">
        <property type="entry name" value="Peptidases_S8_Kp43_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051048">
        <property type="entry name" value="Peptidase_S8/S53_subtilisin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034058">
        <property type="entry name" value="TagA/B/C/D_pept_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399:SF4">
        <property type="entry name" value="CELL WALL-ASSOCIATED PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399">
        <property type="entry name" value="SUBTILISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="516"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="201"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="353"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1331"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="219"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1336"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="525"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="586"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI65615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1881" mass="199525" checksum="E93B955A01375C41" modified="2025-10-08" version="1">MFRQTVAAGAIGRTSQVSTQLLWRLCITITFATIIFLSCIRAQDEDTGNTVVLGNGKVQLSGASDVAAAMAAAATTAANAAGASTGNGNSGGSGGGNGNGGGNGPASVPHGKDKLGRKLILLRYISDDSVWQLRNALLDSGAQVLDYVAYNTLLIQAKAESIGLAARYQALAAEYPPVLKLAAETSAVFKAAAAASAKMLYNPQQQQKTRRQLAAGGVAANNNKPSPAAAASANNSSPAAAASANNNNLPPAAAASANNSSPAAAASANYNNPPPAASAGGGTLLPPPAALSASNVSSNNPFPTGPARGFKSGLGNVATAGVGNDTDASNVSRSDGALNALSTWRMYLKASKPNVKNHTSPGGGPHRPPPPPSPSGSGAANNATSGGPELYAASVRLVPGLSEKAPSTIAEAWPPALAEAVGIKNRTDECWPQVVADGLYSQSSPILLVYLCAKNYEAALTWLSGLDTVVWVEPVLQAGIRNAVAGWIVQTGNISTTLFDNPTSALRPYWAANLQGQGIIVGVEDTGLDMSHCNFIDDRWRTGSLRTLFVGLPPRLYLPDHRKVVQYMLPISSGINAWFGDQPYGHGSHVAGSIAGAAYTNNKTLINQRSTGSAPKARLSFFDLASYSGLNLPMPIDDKILPYHYAAGARLSSSSWGYMGSLGVSYNEVCRQFDAFSWRNPDFLSIIAAGNDGDNGFIPTVTAPSTAKNVLSIGASINHPTGTASSGFNYAILFRYTDAAGKNQSYSMWPYLGSYDYYWRTLLYNKEVPLRISSPLNACTPLIGNYTGAVVMVDLAGTACTPDQRAINARSAGAVAVMFIAVGNGFLDGSQDVIPSISDPNTIYGLTTRAEGLNTIALLSNTTLGVTRPHMTFITYPNINLGVDGLPYFSSYGPFTDGRFKPDIVAPGTGLESTASGPGISQDVDGGSCSSKTVSMSGTSMATPIVAGHLALARQYFRDGYYPTGAPGDPASVGFEPSGILLKAAAIAGARSLEGGFAGNGGFFLGAAPDGYQGWGRLDLSRMLPLPNYTSPFFRVSVADFGVINQGDYIYLAGVEATGTGPIFAALVWHDYPASPASKKNLVNDLDFGYTINGGSYIRTRIDSTNNVERVELSSTNVLAGDRITYVVYGRNIRSKLLTGSDAQLPQRWAVVVVGHFNGTLRTQYNPAYIRPQQYLTDTQVTFGLPGGGCMYVKTDAILSNGNCTTLTANFTLVEEPALPGDPSIGPKLLSNYGYLYSIRDTAGRCITVPGNVSGTRVGLAPCNASDPSQKIGLFRNTQVNGPFYSLVPSFALWSSAGAVARCLKQTSASFIVLIACNDDDLDQRFEIRSSAATRPPRPPPPPSPAPPLPSPQAPAQQWYPYSLVFRADWYPVGKTNLNDYDYLENHNGYGDQYYTDIGTDDLDIIVSWTWGGVSYSMGRRTENAENVTLMTGIEYGGDNMDYGTQYEIVFFQATTKPPPTAFHVCVAWQSTKTPLFRVVMSVFRGYAVATADKVLDTSRVADLICNSSASGYLGTYDVTRPAPPPAPPSPPVPQPYNSSYPLLFRADWAIVSGMRGRPTKTYDLDILVSWGVGNSSYVISSFAQQAGGGNYGGDNMRNGGNYEIVYWPPGVPLPPTNTFHVCVRWNEMPKPLLKVTLSVFKDNLVTTSTKMIDTTLEWSDTCEPSAIGYIDSFWIGTTRGLLDDLSYSNCHSTPEPSPVFGLYILAQWTAPNVTGAGSVDAIFDWDLVVTWRWQGEIYELSPYSRFVGGAVHGGDNMRLIEPTNKEAVYWPTGLTGIEPSPTQYDVCVRWYAAGRLNVTLSVFLYNNLVLNQATVWDGSTVNSKACSPLAVGYLGSYSYNGIIPFQGRRSAQELFGAQELTAGGTGDGALTMDDLRNLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7Y3</accession>
    <name>A0ABQ5S7Y3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="784" mass="81111" checksum="6CAF60BB95D3840F" modified="2025-10-08" version="1" fragment="single">RMLQALLLDSVIGSRAAGGSATGPLPNLLRVVLRLQVSTQPCRTVTTLTDAGPHAIDKISKASLPCCSASGYSDYEDFTISNTRSDLVTGAPWGGLLHRLPILQLQQHQWQQLQQQYYRGISTTSTISYKFDSHLTRYNKAKVANRDNFQRFRDSGRDARVAADILQEGELSDRRGGRRGRSDADKALLELRSMVHDCRDLRSMQVLVRECAGDMDSFLVCSVLGRLPQLKQDTPAAVGPEGLARRVAEALMKRLEVLTPDTSPGNLAHAMIGLANAGFLPQRPLLAAVVARLSANGHRGGTVAADADAKAVSKLLSATAKFLDLFKIQGLPKGPPSSRSTKLEAGGDGSQGDEDAAEGVVGDVLRKQLWPVLLEAAEVKLLRALGEEASSGRAAAAAAAAARRRGNGATEEDDSMPQTALRFICIALALVRETHPGTWTAAARLAARHAAELQPAVAAAVARSYAVAGGSALDGGKAKLFEAVWGALGPQVEGLDVQVQLDLLIAALKLAAGKVSGAATAAAAAAQAVPVLCGLLAVRVPALSVSDASNLMVSLAELHTKSVAHASDAAAAAAGGGATAAPAAVQWQKHSYGALPRLLGDLLILRGFEGFGGPRFASATLALGLLGHVDSAFWRQLAAAALPEVPRMDGTTLSRLMGAFLTAALAAEAPGATVGAAAVTIPAPSPALVAEAHARVQSLLSSDGAPSGKPLFHMVRALAEWPLPGGGGDVAVLSTLADRLLADGVGAGEVAAAPVTVRQRLAVALRRAGLGDHALVARLGVMAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8P1</accession>
    <name>A0ABQ5S8P1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_010040</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000030">
        <property type="protein sequence ID" value="GLI66282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051292">
        <property type="entry name" value="Xyl/GlcA_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270:SF52">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE PROTEIN GNT13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="56720" checksum="16387FAC7305FBEF" modified="2025-10-08" version="1">MLEIELEGMAPGVFVHTCVLVMIILTMSPSYQGLALDSVSSSSRNTSAENLLKQCYNSQFESSGSRFSEYMDGKPTRSLYLHRVWWSTRRAEFPISFFTQADLQRLRALQEQCKSLPGGTVVAAIWLPLGSTIGPRGNDAAADAEAAKLGPGLSARHIPTVKNATAILDNVFAQNEAASNTSCVLRLLLLYEIVFDEALGLLMPINTMRNAAMMATDSQLVAMVDVDLSANKDLAASVMRSPERVAEMILLAEKQHMAWIMPAFDVSPSVPPPMRNYVADEALAVSREEKSRLRDMWTKYRLIFPFAMERGYTRGHNATKYEEWFVSQSHYPVYYEEGYEPWFMASRFLVPFFDARFRGYYMNKLVAARNTLLLANMRVLPDVWIVHRPHELQVSNYGWLEAQKTLQKKHHTEKMVLDQAGGNEQFLRELTPALGHPLLSVCEPILYNGTSKSARDAFFERNIYFYKSAVSQMETRNYTPVADVAADNCRKVLPWWSRRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S997</accession>
    <name>A0ABQ5S997_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009956</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058688">
        <property type="entry name" value="Ig_NPHP4_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058686">
        <property type="entry name" value="Ig_NPHP4_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058685">
        <property type="entry name" value="Ig_NPHP4_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029775">
        <property type="entry name" value="NPHP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058765">
        <property type="entry name" value="NPHP4_C2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31043">
        <property type="entry name" value="NEPHROCYSTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31043:SF3">
        <property type="entry name" value="NEPHROCYSTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26015">
        <property type="entry name" value="Ig_NPH4_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26189">
        <property type="entry name" value="Ig_NPHP4_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26187">
        <property type="entry name" value="Ig_NPHP4_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26186">
        <property type="entry name" value="NPHP4_C2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NPHP4 C2-like" evidence="4">
        <location>
            <begin position="1063"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="domain" description="NPHP4 Ig-like" evidence="6">
        <location>
            <begin position="1632"/>
            <end position="1733"/>
        </location>
    </feature>
    <feature type="domain" description="NPHP4 Ig-like" evidence="3">
        <location>
            <begin position="1745"/>
            <end position="1825"/>
        </location>
    </feature>
    <feature type="domain" description="NPHP4 Ig-like" evidence="5">
        <location>
            <begin position="1832"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="921"/>
            <end position="955"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1926" mass="211755" checksum="09530447E57C6AEF" modified="2025-10-08" version="1">MSGFGLDAEIDDGVLPAPGNNFGRQDSDTGFVAEASLGGGIPPVKRRGAGQSDLGLAAQLTPARRNAGGDSFGLEAETAAKHTTPDEPDPVFVAPDARKGGRGPAGTADTVARPPRRVGPSGGKGRIMPRSDLDSGQVKDNMRRPQQMPPSAMYPSDDLGFTATGPSGAAEDPADLFARAKAQKLLSRQTASRVAQKELDRQMDVMTRRPPTQQERERAVSTIQRYYRGYRVRKSLPPNAPIFRRGKSALPAASAAPGVRGDHFGLTPDQQMMGKVRFDPGTNFEPRRALPTVPIIDIDKMPKYGILSQPLPRAERRAGTFIEYNRDMHTFVKNRDALREALEYKRGYLEQRPLVQWAQFTSRQKHRYAPIPPDANRRALPQTQGYRLRILKVSDMPINTTFDGRTFEFQLGVSLYDEAYGTFYGNTCYSMADTYDRGRAAQRNAIDLDLSFDVYYHTTVSDPRCMAVVEVIMLERMLDGVVKGQYSLGWALLPLFRVSQNPLPPGAPVVSSMQLDDMPGRPMAASLMAGTPRYLMFRNVYNEDLRPPKVIPDATILFQAEVYPAMEPYIPLLPENFLVTYSDTVPGLRRFAASGLLSTTTKTVISTLASPMLSPTYSVMLRRLQLAMPMRVHDLLMSLDARHGMATGYAPPPGTAAAQSPIYQTPSTGTLTYTIRVSVHNGRTFVGPAYELIDLTHLDVPGWKVLQVPTPHPVFDYVMADVLVTIVVELLATPPQAPPPPGLAPLQYLIGYTAFVPFSEIREDGSAAHVAVGVHRYQLRPLEPLVLRDQPQLNWRAMLGDMLSMEVPEPLVEFQLFETARTPILPPLKKVFAVIEAQREEEAEQGDQPLEQLLQPVPVPMEIVPPPEINEVDPDARVPSPPPVGEMVPLERVPSVDLMHSRRSQGSSRGYAMPPELTELTVAMQRQLELLTRAVDELKDERKSLRNSFLSLTDRQGRDDINPRENAAAAVQRYYDAQRDRRGSMQPSPEELQRFLDMEPTRNFGDQAMFSGAQAPDGKLPGSAPSRVLLQQLHDAGMLDALPTDVQAILRRRGPPRGPSVMPYDLAIEARDPRVASDIVVQFLAYRHHDTLNAPANLKSLYLTFQFYHFPPTTSEVGFLVPIRLEDDAVDSTSVIVPADPAKKGAGLVLKFEVDGGTEPVPSGQTAQQAGFEAAMAFAQYLAHKTLHLDVWDGESLLQLGTASMDLQPLLRQGRDFAELLVEVPILDQAAVAPSLQGQTFVEAAYNANTGQNDTKATVISKGSIVLRIINLGRDSRNADAKLPEFDDAHNRPNKKRVRAKPAWEIPQSPIARELAAMGAGMSASPIGGMMANAMGAAATTGGYSGPGATPFGGPGAPVGLALPGEEMKLRILENSHRKSARVQQYLRLEDADVGPGRQGLRESGYSGREDQFRRVQKQLLADVSAARSRRKHDFILEQLRKSMVTRRVVRPAYGEVLVYEYEFQNPYSHEQTFEVRISKAAEVSILSRMEDYRALRAANARFLGTVSQPVGPLEQIMSGNRIYLQAHERITLPFRLQSFEPPDAYKSNAVSPPEPPVNQSLAARVHSSAAAAGRGSPMRPAAVGATAVNAPGALPSGHTVSIEMISTKDDVPVSVLELEVVPRPIIIDRTFRYYQPERQLLRTTVKLRNLPGAQASYFAAAAGALRHVCAACSNPDVILGVGSSALTAGPDRDELYLKYKVGGAPEVAHFYVLLYLDRLMARPVEVWQVFVHALRKVDMRAMIGQTSQASVVVRGVVSRRVAAYNTHPDELQVSPDSFVLVGGALTELLVSFRPVAVGIKDIKINIVDSETRELVYAMLVTAEAQGPLITRTFEVELPVGTVANKKITYTNPYQHYRIFTLRSNQPWLVHFTPPRLQMPAGATRPVGITFDARAATAGMVDALVFVNDDEDKTEECLKIRVRIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9T7</accession>
    <name>A0ABQ5S9T7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_010627</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="18"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="285"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="333" mass="37685" checksum="7F96C0C9A282934C" modified="2025-10-08" version="1">MAQHQDCRMYEAKYPEVDDVVMVQVKSIAEMGAYVSLLEYNGIEGMILLSELSRRRIRSITKLIKVGRQEPVMVLRVDKEKGYIDLSKRRVSPEDVQKCEERYNKSKLVHSIMRHVAETTGRDLEALYDYIAWPLYRLFGHAFDAFKTMVSDEGDAIFRRLEEEKGAAATAVLSPEVREALLKNIKRRMTPQPLKIRADVEMTCFTYDGIEHIKTAIRTAEAQSTEECPVKMKLVAPPLYVLTTQTLDKNKGIEVLTAACEACQKSIELNRGKLMVKEAARAVSERDDRLLSEKMEQLEAANAEVDGDDESEEEEDVGMGDIDVEAGPAFVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9W1</accession>
    <name>A0ABQ5S9W1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010676</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000039">
        <property type="protein sequence ID" value="GLI66735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="282" mass="30406" checksum="0BC6ADE7949E924D" modified="2025-10-08" version="1">MASEDNEADAASCWDDYCLHPQFFHLIERCFGPHDVDLFVPQVNAHLPCVFSRHHCPGSSGVNVLLQPWAGQYASSCLPVDSDFLLAVVQKVRCYSGGPILDSSTVVAAAHGVSSGSCFPPVQHGAIRPRAEGLKRLPAPSELAGRSGPSSVVDWARSRGTRPDSVVFPRYGLNLGSVGAPPVVSDLLVAQLQSYAPRLDHSERLRQVVQVVMARISASTWWSYFAAVLLGGSYFAAFVHFCLDEGFGVPPSFAECWSVVGSVPNSKRQRSKPVQCSHTSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA08</accession>
    <name>A0ABQ5SA08_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyl-accepting transducer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000029">
        <property type="protein sequence ID" value="GLI66274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="40836" checksum="3661FC65768DA58C" modified="2025-10-08" version="1" fragment="single">LVDCAQESADEHGEVQQREAAAAELRATVVELEKRHEAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREPLRLPQLSCGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA34</accession>
    <name>A0ABQ5SA34_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF455 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00657">
        <property type="entry name" value="Ferritin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007402">
        <property type="entry name" value="DUF455"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42782:SF4">
        <property type="entry name" value="DUF455 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42782">
        <property type="entry name" value="SI:CH73-314G15.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04305">
        <property type="entry name" value="DUF455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="456"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="456"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="478"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="602"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="632" mass="68444" checksum="855E1C1EB8926D8B" modified="2025-10-08" version="1">MLPSEGMRSISEWSRGCHAAAPMPSLSAVPRITYDQAALKHPLAASHVSRQIEYGAQRRTEVTTAQGTVEAAPWAPSRPHSQRHLQSQPHEWPGLVTWRAKGRDLRLRWGPPSGAAGPLEVPVAEAPTLESAMALVTATATSKPDQHAGAAVPSLSAPALELRPPGSLVEAGLRVLRTASPACKAALTHAAWGAYLEGKLPLRPPDSPAQQRHQREPRGLQWSGLSLPLPLLLPDHPARPERPRLVRPKQIPGHESSPLGLNGYMLHNLAHIELNAIDLAWDTVVRFSPLGLPDQFYEDFARVADDESRHLGWCLQRMEELGHCYGDMDAHDLLWQGCRASSVDLGARLAVVPMSQEARGLDAGTRLVSRLVGFGDSRSAAVVGLIAEEERAHVAVGVTWFSRLCGTLEIRPDELYRQWLLHLNPDLLKGPFNHSERGRVGLPREWYDPTVWPERADEEAGKGDAEQLEDEAGGSGTAGSNTSSSTHASTALTPPSSLSPSSSDQGARQPRRPSRGSPHPRLKGPQPRLTLEQLAVLAQRLSVFGDVEVSSAITANLESTFPHAPTLTCVHADAHTLPNSPHGMQSGYLSDVPSSQGSTDPHLSEMERNGERNRRGKTMHHGNGSLPNQESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA85</accession>
    <name>A0ABQ5SA85_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="155" mass="15736" checksum="493079F21C70A2A0" modified="2025-10-08" version="1" fragment="single">LVALTACCNGIGTFSGLELTFSSGRTEPSSGCSHTPFPPQDVGSPGPARFTVDVPRGAWFGAARASAGAALGSISFGFVSLTTPPPPPSPPLPPPSPKPPRPRTPRYFPPPPPGLDLPPPPSPPPPPTPPPPLPPIPPSPRPPPIPSRPPSPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAP6</accession>
    <name>A0ABQ5SAP6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010222</name>
        <name evidence="3" type="ORF">VaNZ11_010226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="16342" checksum="A457A12351A9E51C" modified="2025-10-08" version="1" fragment="single">TTMRFLKPPMYGSCWKTMTTRVAVFSWRSVSRFSVFGRDASTAATSTDMFGRKRRRYSDGGNDSASEANNSLLAVTDQQREQLAAPDGGEDASNNNISLKSITALHARKTTLMRLAKPASGSGNRGSARDHVDVVAQAVVSPWTTLGRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBC9</accession>
    <name>A0ABQ5SBC9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_010328</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF246">
        <property type="entry name" value="DEXH-BOX ATP-DEPENDENT RNA HELICASE DEXH4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="143"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="460"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1112" mass="115677" checksum="C44BE56A4337D0C0" modified="2025-10-08" version="1">MDSVEGQELPEEDVEVEDGEGEEEGSDTLAEPMNHHDGDAVEQEEWEDMVAIGEPAQEQAQEGVVSEQASGEAVAAAPAAAGDGDGDSNAEPHSQARRGADAESAASTSLRAALERWAGSPAGRAMAAARAALPIAAVRGELLEALRQGDVVVVSGDTGCGKTTQVPQYLLEDAIFSGAGGRTHVVVTQPRRIAAISVAERVAEERGEPPPGSPGPGSTTGYHVRLGAAVTRHTRLTFCTTGILLRRLAGDPSLRGVTHVVVDEVHERSLQSDFLIALLRDLLVTRRMQLQAAEAQEAAAPGAAHSDGNGAAAATAAAVPFGQHSSVLKVVLMSATLDAKLFANYFGGCPVLQAAGRTFPVSRLFLEDVYEATGYRLASDAPVALRRHGPGVAHVYAQRLAGGSRGQRDLVAKGFGDDEALAAPLNPEYDPDLYVNLPLHVRRNLARLDEHRIDYDLLEALLSYIDATTEPGAVLVFLPGIGEINHLYDRLTAQRAYTGLRGGGPAVSSYSSARCVVLPLHSAVPPAGQRAALRPPAPGLRKVVLATNIAETSLTIEDVVAVVDTGRHKERRFNPARSMSMLVEDWVSAASAQQRAGRAGRVRPGVSYATYTRARFESGLRRYGAPEITRVPLEELVLQILLMGLGPVSDFLSQVLEPPQPRAVTIALEVLRQVGALETAPPAAATAIAAVASGGVTAVHNSAAAGGATGAAGGVPPATAGVQPSIGPPREVLSPLGRQLALLPVAPRLGKLLVVGALLGCLAPAITIAATMSHKSPFLTPTDDRGESERARRALAAPGAEGIAAGQQSDHLLMVAAYELWRIAASPKYGGGTRVAAQVARRHFLHLQTLEQLSEIRCQLAAMLADARLVQPAGERGGYGSGYGDGDGSGYGGGGGKAAMAAAAAWLDDPTAPWNKFARDPLVVKAALCAALSPAVAVMGEDSCPTSPPRWGDAAPGPGAGDEVFLHPSSVVSALNTPQLLHPYLVYLEKVKTARLYLRDVTAISPLSMMLFGGPLTVLHAEGAVLVGSGSGSGSGVAQSVLRIACRAQTAVLVKQLRGALERLLERRFVGSSGNGGGGDGGGSQVAETVVGIVRQMLREEDEQRCMAARLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBF4</accession>
    <name>A0ABQ5SBF4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF247">
        <property type="entry name" value="GH16156P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="82"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="389"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="872" mass="92541" checksum="704167EC8BE95E07" modified="2025-10-08" version="1">MAPKKRQAKASIRKKRILGVLGAPIYDSDEDEDWKPNDQSSIKFEPIFESRQLRQERREQAVQPAHCETGLEDVPLSLRRRKAQNRSGICDTTRVAEQTDTGLPHHPPDERKRRKRGEETRALSTSTHARSTSVGEAAAPGARLDWSCLTEPLLVAVLRHACAVSALPTAASAACVCRHWRDVCKNLEETVKAAAAAAAAVALVEAEEEAEAAAAEAAAAARSAAMAVAAAKSVSALRDVMGDEASMTTTDASTTTAAAWMELGGGVGSSGRSPCGGAGNLWSCLDVRSQPSIHFTNEVVHRGLASGRWSQVQTLKLRTCTGLTPDGLLALIGGCPGLTCLELCQCESLVKIESMRAVLSSLPRLRVLDLSHNAVGVMDHLLKLALAPRHRPHQQQQQQEQQGRQQVVKASARGEGSAAGAGGRSSRSAADGNISGPSMLPCPLQVLRVRGCARLGPAALRALSPSVPYVTFPSQQQQQQLPPDDGVAGAPAEPMLIPLFVHLTELDLTGSGSLTSGGLLSVEKLQAAAPNLRVLRLDATASAQHSGLRLAPEPESPGPGFPRLRLCELGLSRIDQGMEQHLSRRQGCTVNMLRRLVGGSPRLRVLDVSGTQSIEDKRLLDSLEADVEADTAAETRAAAAAAAESAAMAVYMRPGRDDRPTGLFKMFPNDPEVTELRMARSMQSLGPALQPVLVGPLQVLRARKSVLATDAGLHALAARFGGTLRVMDVSESSHVTDAGLLHVALNCTALTALDVSSTLITNFGVRVLMALRYAGSEARMAKAGSVSEADDMRPSGSSGTATAMKEAAISRHAPVVHNLVSDDDDEGEEVDGVGTRHLRDLNINSCRGVDRSTRHAAAMGLDTLWRALNHNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBL3</accession>
    <name>A0ABQ5SBL3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GPI mannosyltransferase 1</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">GPI mannosyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_010691</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of the glycosylphosphatidylinositol-mannosyltransferase I complex which catalyzes the transfer of the first mannose, via an alpha-1,4 bond from a dolichol-phosphate-mannose (Dol-P-Man) to the glucosaminyl acyl phosphatidylinositol (GlcN-(acyl)PI) intermediate to generate alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol and participates in the sixth step of the glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PIGM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="BSDZ01000039">
        <property type="protein sequence ID" value="GLI66742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007704">
        <property type="entry name" value="PIG-M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886:SF0">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886">
        <property type="entry name" value="PIG-M MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05007">
        <property type="entry name" value="Mannosyl_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06728">
        <property type="entry name" value="PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 11" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="442"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI66742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="531" mass="58679" checksum="1CEC373BB3841138" modified="2025-10-08" version="1" fragment="single">ARGGSPYQRATYRYSPLLAYLMLPNIWLHPAWGKVLFSVCDLAAAGLLDFLLIRCGASRSLRSTALLMWLFNPYTATISTRGSCDVLSALLLLGLLAGLLYGRPVMSGALYGLAVHFRIYPVIYGPSIALYLVRRALVKWTEKQEKHDCSNEVRTAAGARTVAVAVQGSAGMMYDGKDIEPHQRRGDLRQGAAAVVSSDSPALVQRTSAATRVVQRRSTLVTWAAAAAAAMQPVVLFGAAAATSFSLLGGLFYKLYGIEFLQEAFLHHLNRKDPRHNFSPYYYPVYLSYGNTSTTMYDSESLYEDIVEAESSVSPPLQPLLHVMLAAWRTVRRVIPVYEPWRLAMVPQAAGLLALAVRFHEDLPSAWVLQTWCFVTLNKVVTAQYFVWYLSLLPLVLPALAAQSAVPSAALVAAGAVWVVAQLNWLAWGYKLEMKGQSVHLPLWVAGLLFQAANTGLMVLLIRALLPRKLSRHRRDDGRGDSYSGGTGSREDGIEGRAFVRTTYLEASLLLCRRFPTREIVIPDNDKRKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC82</accession>
    <name>A0ABQ5SC82_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="296" mass="30433" checksum="C7FBB2790050FA05" modified="2025-10-08" version="1" fragment="single">ARYEGQSANSTAACTSAASRPAAVAAPVAATEPASDLRSSGGIRRSLPRKCGAAVRSMPMLVPSAPMAEAAQAKNSTTTKIRGISFNNPLYSRGKLAVAGSHDSGRAAAAASGTRKPRTSSRLAALATATRTGLASLWPSRTSPSWTSVRPPTSTGVPSQRGRQSTSRSSPQSLRTFPSAADGIAAVPQQLDGTTTSVMAPQDLSVTPGSSQSPQELSSSGGGASQPSSGINSMAYERGIGRLPPLRVRRETYAQPLPAVRSRKGAAESSGESGIWRNRAVLRPVTMPKVRPAATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD36</accession>
    <name>A0ABQ5SD36_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_012068</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="7"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="132"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="298"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="244"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI67815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="415" mass="42940" checksum="56A4F849D4FD8D6E" modified="2025-10-08" version="1">MSGGAVSNAVVEGCSGALGSVIALLATYPLKTIYTLQALSTGGSSVSEAEADVSSRLLAVARFLKQYKVCNRQVLLYVHKILSTLYAGLGPNVVESALSSGVYFFFYSKLREQAVILSKRGCSAAGNRSKDIGVIASLLVATTAGALNQLITMPASVVATRIQGHRSLTKRPPTTWETIASVFREDGLAGFWKGLLPSMILLANPAVQYMLFEKIKALLKYWKAQRLAQAEKMSDEAAAAAAATAASAQQQHDQKELPRRCSADSDEAMEAATAASKAAAGQRQAAGRAPPSSGSVELSAGEVFLAGALAKIGATVATYPLIVVKARLQASSSAASNSSGGTAYRASTWGVVADTARHEGMGGFFKGLRAKILQTALNAALMLMLKEQLHGVTKTALTCLTSGPAGAAPAPAAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEL8</accession>
    <name>A0ABQ5SEL8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF13">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047441026" description="Methyltransferase FkbM domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase FkbM" evidence="2">
        <location>
            <begin position="183"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="526" mass="58502" checksum="B5ADCBF0FA0C733E" modified="2025-10-08" version="1">MNMYNNMIMLALLGWLLLGLPGVPAAAAAGLRTHSVAHATNLPVREICTDTCSKAKNGVCEEGRPGKVVTNSPFRMAYCDLGTDCDDCGPWKTTSTNISWIDGKKAGPIRLLQSRDVQVRVKNAAVPQEIGFSFAYTDPKQDFDVSYHMENAGMVEAGITAIAYKLMSGRCVKQGGNRELFVDVGANFGWFAILAAKLGCKVIAYEPVPLFRAFFEFSVHLNDLAFLIDIRDTVVSHESDTLMKMVVPARGIWGTAGIDGLNIDRAIQSDQEAIEVRSVRLEDEIKDDVLLLKIDVEGWEWSVVKGAEKLLKKYHVENVIMEYSPGVPERHFKWDHMAATPAMLVDLITKFEYRIGHIGDAGKHQSSGWDSKLIPLKEITMENLKYDVEDIKLWQAGTLACPVPDELKEFEMWALCGGVPEGLNPRSLRSEIGHNTNIWASKGDGLKHLILHGVVGILSPSDPTEKYFQDNEMNFGMGSRPCHFLDPRVLVKHRCKCTQPEVCGKEEEAVRKAAAERRIPQNYVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEM9</accession>
    <name>A0ABQ5SEM9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="459" mass="46769" checksum="E3658F9D10165155" modified="2025-10-08" version="1" fragment="single">CPQDASSSQAVHRSAWNQRETDSHMSVLARSQWFCSLVQDALPDLSVDTASGELCWQGVPLTSTPVLDRYMAAAVCAVCLAAREQVTILAKPNLSPSQPSWSASPPTQKTVQLLSALQEALLLRLLPNLVACKAPLAGRAAAAAGGGSYLGQSTALEAVPCVTSAPGGGGNRAAGLLMNAHPAAALLCCAVADLAVAASESVAGAPYLSTTGGPANRDNHPNRVPRAADNAPGDGRVGDEPLKRLVTLCCEALAGFAEIEKEAEAGARGSGSGLSVGQVAASHTLRTVCVALASDLGACGDGEVRQKMDGETCGEGMRSGKPGSGGVAGVLQMMLDVVCAAPQRLGTVLRQHLHALLLPALLGACAVQMSPENYAAAAARCIATMRGDSPTNLRNQTCTATAVLPPSLTVTSAVREAVATLAGLDARHVAAADWPLGCFPPCSPRRCWPRGIGQPGSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFK7</accession>
    <name>A0ABQ5SFK7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Orn/DAP/Arg decarboxylase 2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012855</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00622">
        <property type="entry name" value="PLPDE_III_ODC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="ARGININE/DIAMINOPIMELATE/ORNITHINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482:SF6">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="5">
        <location>
            <begin position="184"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="566" mass="60856" checksum="9083B2871C66163C" modified="2025-10-08" version="1">MTHMEVVLPTNSHIAEEVVGTDSAGADSTVEGGDFPALATQTSLSRTCRSDVGRTSIGHHTSGALLDIILEADAKAALAASVQPVDRGPAGPAASSGTTAAMPTACPGAPMPPSTETSLSLLSSWQYRVTGNGAVAQVMMDFGCEHIKSGGPRGIQEVAIRHILRHDIDSDSIYCIDLAAPFRLFKAWRAAMPRVLPYYAVKCYPEPAILKLLMALGAGFDCASKGELDMMLRMGVTPGRIIFAHPCKRSCDFQYAREHGIQFTTFDTVSELHKVAEMDPEFKLVLRIRADDPGARVPLGLKYGAEVSEASALLRTARDLGLECCGVSFHVGSAAKNLATFTNAIENARKVFDEAASLGYRMDLLDIGGGFTGHFDELGNVMFGEIANTINAALAKYFPPGGELGDVRVIAEPGRYFAETSSTLLTPVIGQRDRVAKDGSIHKDYWLTDGLYGSFNCIVYDGQNPEYRVIRSPVQPEPTDTRPYVSTLWGPTCDSADVVYKDVTLPALRNGDWLLWPNAGAYTVAGACDFNGIEFTHPCKVYVWSDSVVDPQEEQEQGGEVAGEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG07</accession>
    <name>A0ABQ5SG07_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013427</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039190">
        <property type="entry name" value="TTC14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23184">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23184:SF9">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="360"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="394"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="457"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="457"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="539"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="555"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="573"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="601"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="627" mass="68263" checksum="42DD98074EC81EBF" modified="2025-10-08" version="1">MENDTLERTTNSLRTLLLEKVPDILLQAECGGGLELPTPLPWGFANIPSLHELADIAKQELVEELRAQENVASRRWRNYEQPSSSNPQDISGGEERCLCEQQQQQQQQQEQAQQFPQSATRQHDVDDYYHPQEGKGVFSETAAEADREGHEGRPAVVHRNSPADGRPLDVPEEACGRIEAGGRHLGPDIQPSVPRANQCFSAASNDEDAKEDGRAATGDWAAEASAREAAGTPGKLQVAACMLEPCNDEGDPVGAADEGAGAGGTDRCQDVPHRIEDPSLLHESDPISPCKPYACRAFLDALFQSRTFCSPHCEGAMAEAMSMTDMYDGLAGSTLWPPPRREDLEVLRCLLKDHINSRWALEAVKQGVAAAKQGNYADAHKAYTRALELDPGNAEAYVARGAAHANQRAFPDAERDLRRALELDPGHRNAATYLDAVLRHVAEQRQQLEALEEERQEVLRRQQDQEQDRRRQTVAAAGGVNGNGADGRWTAARPMAAADGRPVGQGARYGEQEPPSAPGRLQRDPVSYLALQSAGRPGKGAEGRRRGGTDDSDDSSGSSASSSGDGDNSDGTPGRGEALGKGGPLKPASDDIRRALEVVFKARKHKKKSKHKKHKKKHRKSKRRRHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGW4</accession>
    <name>A0ABQ5SGW4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Zinc-hook domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">VaNZ11_013765</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004584">
        <property type="entry name" value="Rad50_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00606">
        <property type="entry name" value="rad50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75712">
        <property type="entry name" value="Rad50 coiled-coil Zn hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="13 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc-hook" evidence="21">
        <location>
            <begin position="675"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="277"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="508"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="749"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="871"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="286"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="524"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="719"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="722"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="GLI69195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1346" mass="150165" checksum="5642EC8EEA9DFD94" modified="2025-10-08" version="1">MCTVEKILIRGIRSFSPENEYIIEFYKPLTIIVGSNGAGKTTIIECLRNATTGELPPNTRQGQGFVHDPKVAGETEVKAQIKLSFKTATGQPIYVTRSFQLTQKKTALQFKSLDAVLSCRNRNTGQRESVTYRCADLDRMVPTLMGVSKAVLENVIFVHQEESNWPLSEGKVLKEKFDDIFAATKYTKALEALRKLRTEKSHALKECRLGLETLKQVRDMADHHSVQRDDAKSRATDCQTQMATFDMKIRDLEQQQSALMSKIGEIDSMAKGMGIRRGQLDQLKATNREREERFRHEGREEFEEDDAELQSHLADSERLAGEKQKRCTSLESEVEAERSRKDSLAAQYQRDCLRHGQLAGEAATHASNVAARDRAVRATAVALALPLPMSMDEEGLADGGMGGRDGASAFSPAATEAFCENVAAKVRELDGRVRALRADARTNESRLSSAVDVATAQLARAQEGLRLKRVQLEDNRIAISNCGAQIQCSQYTEVSARALREDAEIAEEHYRQKQAEESSSDAGRQVEEARREVETAGRRMSELRLERQRAAAAAEGSAKLRVKRTDLESKEEQISKVMAARRQDITKVVGLQSGEAVPEPGALRARAEALLSRLRAEEAEQLRTSQEAHRAAEAARTSLRTRTSELAVCRQDMERLRAELRYVVANALGEAVVNEDEYNNRVAAVQKAEDMSRSRLGRGRATQILISNYKETVAKGNPCPVCDRDFTEVERRKCLEHIEHDIQALPASIRECETALKQVQRQLDALRALQPTYVRYNDLKAKLPELEQLEGQLREQSDELSEKAEVAQMDYAEVHERVKELTRLNGEVVWHIDRLAGEAEALRREIAHLDPATSLVGSASGGAASRSVGAIDSELEEVEAARQRAELLRDAAMARLNRLRDEVLTAQRGAMAAQQRALEAAAAVNRVNELQNKIRELEVANAALEEEWRSAAGSLAPLEAERDSLVRQRDEARQRSQKEVAEADEQLRAAQLLQSQLLDKALVVSEYESRGRGAELARMVSELEAANGRIEEQSRALAVMEERLVVLRQELVQDAALRRDVADVLEMRAGRRAEQEMAGELADLEARVAQVGDPGALQRQAASLAEQRDALRSKQDVLRGHIAAAHQAASRAQAELNDPKYRDVHQRFRTQQLVVKTTEMAMSDLDKYYKALEKALLVFHTTKMADINKIIKELWQKTYRGQDIDYIQIKADTEGVGTRSYNYRVVMYSGAAELEMRGRCSAGQKVLACLIIRLALAETFCLNCGILALDEPTTNLDAENSGSLARALKALMESRAGQENFQLIVITHDEHFAQLIGTREHVDTIWRVTKDPATQHTLVTPEALQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHZ0</accession>
    <name>A0ABQ5SHZ0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028790">
        <property type="entry name" value="MKKS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46787:SF1">
        <property type="entry name" value="MOLECULAR CHAPERONE MKKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46787">
        <property type="entry name" value="SYNDROMES PUTATIVE CHAPERONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="539" mass="57945" checksum="6282D8979CC3DD23" modified="2025-10-08" version="1" fragment="single">LYHMEALLQICKFYEELALSCFGPYGRLKALQAAPHTPASVITSVSGPLVQGIRASHPFGQLLVAGTAQAQHAQYGDGGLACLYLAARIIQAVLENSPCRSDLYESIRVLQALGHMVQGLLSSDFGNAQTPVRTTVQIVACKSVQQYLALCRGVLFPKLGCFLSTSDLEHLAALMVETFVATLPDEHPDLMSAASLPCVSIQTFRGEDVRESCVLPGVVIPRSDIAGESFDILVDIAVRQGNADVMPHGTVNYTIGDIGILLLREALDVRGSQHYDSASTGLDSLIAVQQQPWEQPGEAEAQEIQRLLTMLDGLLQKSANIHVVASQKGVNPVVQDWLVRRGITPLQRLGAMHITALGRTAGCTPVDSLLHCHGSSTCGRLHAEIKAIGGHQVLHLRPTTHSHVVTVALFCPMDALSGHIKHTAESSIHLLRTTLQQRATAVQGSGRIENALAIHIEKWAAGLSGKSVRQEVVICSVALALRQLKQAVTLRSSCSTQSQGDIEDFNPYENSNGNMQVSNLDSLPCKISCLQRAISTAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI67</accession>
    <name>A0ABQ5SI67_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1177"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1297"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1529"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1852"/>
            <end position="1880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1939"/>
            <end position="1963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1975"/>
            <end position="2010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="610"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="642"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="730"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1311"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1575"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1944"/>
            <end position="1959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1982"/>
            <end position="2010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2257" mass="232811" checksum="4CA63A4BD568AB2B" modified="2025-10-08" version="1">MWSNVPNGGRGRQMTDKTSLTKATGCTSGIDSSASTSGRGVGASNASASAGGAACPTQLEPIDDDLPPLLSDNSYSGSADEGGHEEDEARARRFRHGDTDDSEDYDSDKDTYNESDQEDDRYDYMASFMYEEDPSLPAVHRGGTAAATPGKAAREPLDATLQDVPDDDEDGSLPELVSSSDDEPDPRLNPFARAASRCEAVPGTAPAAKAAPILRKGFFDAHKVAATKKAEPASVKTGGKSAPQPKSQSAQLPSSRPSMPYSNAGVGIRENVRSGLPQRNKPVDAGRAQTHSAQHCSYPARAAAAAATPDDDDDDDDDDMPMLLHSDDDELDVYGADSSSARQACTRNMAATPAGAAAAQPGLASSRVSAPKAASRSAPPVPPAAQQRSSTRRTKESRKGREAPSFHEAPMNRSDFTNDSAWQQHLASEREERKRLRRERGLVESEDQGAVADIVLGYCCLRPGNDTDHLCDEPGYKVKETQRRYHLQCTRGCSLLFHDKCWRPFADGIAQLRGDEFDRNFKANTKEGKPKLHRCVTDCCDGHISRVYIFQAPEVPGAKPGHELYCMDPVARQLAAVAEAAKRRKRLANEGEAQASAGSNQQATKKSRKAAGASSRAPAAPAASATAAARAARREDRRRAAAEAAAAAAATGAGQDGGQEGTAQPETAQGPTASRPVVPRGISIVVRARSMQKDELEGPLRGDVNDESAGSRLGEDWTAPPAPHCETDFSESAQQTEAAAPRSSTGDCHGDMGTGTSPLPPVLAATAAASTKKKKKGYTMHLDEFNAVTGGGGGSGSGGTAAAGTSGAVSAATAAMAAASFNSEAAAYALAAEEGYHMPQLQPLAQRDVRYSGFPTLSAAMEQWARPIDVPQEDYVMEALSKLRLDERQGGSTNIILLERLDVRFLEKQQTGSEGYLRSELETYGGVVRLHVFREQECAAVEMEMDDVAYNVFVIFRNRTIGSKRPLVCFLRDFPTEEVLSRHIQAREEGIQTVDRVRPDPRLATKETWAPMPADGVAASMAMLWSPSTMQPKVVNAAQLDEDVALGTSFPARAALLRGPIVAGKSGLPAGGLPPRALRAEEASPSRLSPSVSAYIGAVAANPLTIPTSRKKVLPIIDTNTREVVNKEAVESVGSAGTPGTDPVARMAAAIGGGASRSAAGPGAGAGVPEASADSCEDAFARAQSAEPSLQKYEQDQQPLLRAADSSESPEDGAMFGHAGTRHRMGTDVKMSNADQESMDQTAGAGRHAMGINEHAQEFVGVEAEARTQPLEAVTAPATRAAMAAVGGLTASLLSHAPGSAAPDADVSQQRLDKERQEEHGTPPRLKAEGLNGESTSLTVTGANAVADVIDATAVAIAPAGNGSESVSGAEGDACDARSAEHAEGGSGAVAAFTKSVLAQMFEDGIGAPLPGSALPQEGTRADEVALSAGVQHLQPKAEPSLGAPTADFAKVPSALANTESASELAPLPQATAQAVLKGCAIMPLVVESAVASTAVECVITTNDPAGQEITSEDDFTVTAASVLTGAMLDPGGRSSLTAHGEDLGSDRAGALSSATDASQRLIGGHGGGGDGTVAASTARAAPEAPTDPVAAVGPAPGPASGTGHGILLQKLIMAGLQGARPLVAIAGRGPLCNGLSPPNMAVATASTVSTASGAANSTPQQPADASHSIYLDDFWQEYDIVYVINCETRYLSTYLQCDTMIDAVFFPNWDASVPPSRVALLLFNRDTDEVLGLWAAKGCDGKHVTWYLVRRLPAISKHQFHATSAGARVFPGLPYTTNSARALGQDTRDVLRVLAMHEATTSTGSLRAPPPVAGTSVVTAMPASNVRPSMGPTIFAAQHVAAAQVTTTVATLKPPSPSSVTAAPPERQHGAAPHLSSLPHNLLGRFMSQTRAAAAAAPGSGSATTATHLAATNAVVSQSYAAASSAPNVTAHSDLVEASGAAPAPPPELAPPLPPPPTTSFRTPLMMYLQQRVAAGPPGGADPANFSSAASAVPPGPSAAAPPAAESSPLGAPLVQAAAAPLAASVVPTAMIRPPVVPPAVVPANSIRPTAIGLTVRPAGVPVATGLPAVRPVTAFPAQPHIRPAQPVATSTQPMQPSPLPIGQVPIRVRPAGMVAAPRLVIPGMMPGSHATAGGAPALASIRSQVLAAPGAPTAPVAPLAASVVPGCTATATAQVGAAPTATAPLGQLARPRQPANSLLCAMCHQRTGGWVLLDCKHMAPCAECCTDPSMFGQLYPVCWCGANTESQRTHKVHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI81</accession>
    <name>A0ABQ5SI81_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="14315" checksum="AE42BD9ED517229F" modified="2025-10-08" version="1">MGSLRTITTYNPVTHPQDSLQRLHTLHNLNQLLVTALAASASMPDALTAISRTLRGHTLSEDDATMPQAVVAHRQGMRHTRSSFRRSLWCAWCSGFMSCFWKTQQWVLVFGYSCVYFSVCLYVESSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJB0</accession>
    <name>A0ABQ5SJB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">KOW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014150</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_B">
        <property type="entry name" value="Ribosomal_uL24_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_uL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057264">
        <property type="entry name" value="Ribosomal_uL24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01079">
        <property type="entry name" value="rplX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17136">
        <property type="entry name" value="ribosomal_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="169" mass="18307" checksum="D79E9F2909763968" modified="2025-10-08" version="1">MASLLGARGPAFFGSRVAIGKTGAPRRLAPTTTHVVAAYGDLPKIGGGRKWEHYELGPSGKALRVDMHVKKGDTVQVIAGKDKGKVGTIVKVLPKKGKVVVEGVNQGQKHIKPRTENESGQIKTLEFPIHHSNVMLYSKEKNVRSRVGHKVAESGKKVRYLIKTGEIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJJ7</accession>
    <name>A0ABQ5SJJ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycerophosphocholine acyltransferase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_014175</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021261">
        <property type="entry name" value="GPCAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201">
        <property type="entry name" value="OS01G0585100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI69532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="109" mass="12741" checksum="5D7D0B4AD1F88DBF" modified="2025-10-08" version="1">MTVKIQQRGYRTLFNYVTSQKKGTFHAIARRVAPRFQPPVYLFLHLCFCATTFAVALLCWYSFWVHTALLAAVASASIWHGGSYYFEVFARRYHEQLLPQTSPRKDVQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJX5</accession>
    <name>A0ABQ5SJX5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015222</name>
        <name evidence="2" type="ORF">VaNZ11_015225</name>
        <name evidence="3" type="ORF">VaNZ11_015234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12422" checksum="8EF7E3DC1D9B3FAE" modified="2025-10-08" version="1">MIDVEKAFAKLDSQAKAQSTFMATASKCFDLNLWVFRLVGCVDTSKLAVSLKAYVKIPLLGSKLMGSCTVSLSSGPGCLLGYPKVASIRLYFKGRSLMMEASFLGKSTSFTVFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJZ6</accession>
    <name>A0ABQ5SJZ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Caleosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015257</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the caleosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007736">
        <property type="entry name" value="Caleosin-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31495:SF0">
        <property type="entry name" value="BINDING PROTEIN CALEOSIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G13750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31495">
        <property type="entry name" value="PEROXYGENASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05042">
        <property type="entry name" value="Caleosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14737" checksum="FF6564E2869FFD3A" modified="2025-10-08" version="1" fragment="single">QAGRVHRNMDYVGIFRADLPRAYQAVDKHSPAGTEGLSIPTMTVLQQHCGFWDRDNDGRIYPMDTYQGCREVGMSVLLSLGFTWLLHFTLLVPYMTQDSWILNPYLCIYLKNIHRIKHASDTGVFDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK65</accession>
    <name>A0ABQ5SK65_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47689">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47689:SF2">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="169"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="660" mass="69818" checksum="E15AB755A8B1A4D3" modified="2025-10-08" version="1" fragment="single">NCKEMVTGRAARATLAFGTFCYSQYPYVSALPFNRLKGFCRVHVVPGCDQGGVPATTNTGNWAAWLLGIAVMAPGTCNADDRQSSAGPSSHFPTVAVPVSAAGNGVPGFTNHPLEYDPVTNEHTAKMTLARTAALEFYNDGKYAQAETKFREAVAEAQLGFEENDPHIASAKNNLAEFLRNTGRWDEAEALYKEALDLLDRNFGEKHWLFVAALHNLALSYEARGDLRAARATMERVLSLRLSMFGPHHFLYADSLFALGHMLRGDPKHGPDGLRMMSEAVRLLEEAERVQVSVVLHWLTEVANAHTADGRPDLAATALRRAVAHIATERGDEAAAASALNEQLTETLAASGRLLEAVQTAEAALSARRRMFGERALVVAQSEIRVAQLLLAAHLQDRNPAATPKQQLASEGLGPSLRALQHAEAAARISAANVDECNQRGMLSEPPPDRLLKRLRAAHTLGSAAKLVATLAGVGDSTAVATSPVSSTDSKSAANSASGGMSLPSASTGPPQAPRRLPWWPFGGNSNGVPPTVSVEASSARQLAPQVPTPPAAPVAELALADARLEQAVAALTGALQAVGAGRRGLPQGVSADELAGLLVETQLQLLDVLDRLGLVLRARVAAGQVVAAGTNEAGGSGDVVERHRRVLEQQERVALELLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKZ1</accession>
    <name>A0ABQ5SKZ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="14673" checksum="9B6B0046312EE418" modified="2025-10-08" version="1" fragment="single">LHPDLASAGDFTEEDAKLLVQMIDVEKAFAKLDSQAKAQSTFMATASKCFDLNLWVFRLVGCVDTSKLAVSLKAYVKIPLLGSKLMGSCTVSLSSGPGCLLGYPKVASIRLYFKGRSLMMEASFLGKSTSFTVFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL12</accession>
    <name>A0ABQ5SL12_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF171">
        <property type="entry name" value="SPORULATION PROTEIN SPO15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2063"/>
            <end position="2093"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="170"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="318"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="469"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="594"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1541"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1695"/>
            <end position="1771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1811"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2007"/>
            <end position="2058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2175" mass="233300" checksum="3AB3DDB986E624A5" modified="2025-10-08" version="1">MSHKTQELKNLGADVLAKFKSKRATHKTAGSLPVTPRSVSVESASVAQAPPATYQERLQAPAVVVSPELTTPASDKPPSPSALGFEGGTEPSALSFNAHFPLTADVTQQVALKPERDASAAISVADGLQQPSVAAPLEPETNAVEPMQVAVGAGEVKDTETAAAGLVSIMVEREAQLVQLRAEVERLRSEVAEVSVSKAELAAHVEALSSARQALEAEVQLLKAQLKERAQTQAAEARIAESDVAQARQAQATLAAEVEELRDLQWKTKEELAHWEVLHRELRTQLESKDRELSTALAAVGELQQLRLQLGGEDASSLVARAQQASVLEAKYEKLKEEGKVRFDKLKEQNNNRKAELAEARHQLVALAEEKAALEARLQTTTTSADDAAGQLQRQLSDANAERDQLRVIADRVPELEARTCELQVALASVQDALAAAHQAVHVEAERRAAAETALEAAEARHSEVTAGALRQAEAAALAERRVAQLEAELESARRAAVEVECRVAQLEPVEAECAALCKNIAALEIKAAAQAAELAAEVQRLTKICESQQRELADGTVGASSLQKQLTELRAELETRLDITAASAAAAAEAAAQERNEVALAAVKARLADLEEELSKERAQLAKGKAAFKKAKEENQRLKEELAKAREDLSAANTALAAAEEQVALSAAAVEQSRQEASKWEEKAGALSSEVAEARSESEAVGQRLEVAEASVAELRVQVESLTSDNEQLQQQYSEQLDVQDQMEAKLIQQAAELNALRIRLQEGVDKLAALEADSAAAAASAQQAAEAAAAAQSTAEENIVRLEQRATEAEALAEVRAGELAIVQAAAEQARSKVADLERALMEAEAQAAEQRAAATAEEGRVAWLTQQLKEVQEATEMQKQLQDEAVVSLEAMLAATTQKLTEIEAQLAEAHQGHQEASQLQARVGELETKLGEQSNRMATAKMQFGKMKEEKAKLKEKVADMEANIEALNSAKVALEAQLAEAQAAAADLSAFSSQVEQLQERCGELMVTTDSLRTQCDEAQTALQAAEQRAIAAEAQLAAEAEETISLKAKIEAALAMRQASADALTKAEAELAVALERLESSRQQVAALKEAVDDQQEEIRELQQRADEDRVATAAAVADADTARLKLEAAQSEAEELRRGLKAARAELEVRSAEAASEIEHSDNLRQELADLRAQMFAVQEASEERIVALKQQLQEAQLAAEAATAAQSAAEENIIRLEQRATEAEALAEVREGELAGMQAVAEQARSKVADLERALMEAEAQAAELRAAVTRAQEAEMERRQKASEVSVALEAAYQQVAEAQHSEQKAAEELAQLRIEVEEVRAEAAFKLKQQAIDMEAIMEARGLELANTQALLEEARSRVEGIQQELGKAEAYSAKLKAVVDAQEATIEGLQGEIAQATTQVDESRQQLEELQRSSQAAAVDAAAEVQDLLRQLHEMRAGSLAAAAATSVVRAAQAEAVASHEEAERLEAALADGGSWDANEFDNQNGTGINQTISHGFMAADANANEEGCSAPKVVLEESQLAQAITTGEIQSLRDALQDSQARISDLEGRVLSLQSELEEAQSAAMDAADAADRCELLQDACKTLRTQLEEAVLERKLATECASEEVKELQERLGQLAAEHAGAISQAALVAKLQMEVAAMREDLEAAVAREAEATIAMQTLRQHVDQEHMADLEASSVLQRRCEQLQAELDESSYARAQLEAEAKALRCQLDELCDGNEHLLSELREARAALSSEQVKLTAAVQRVGELEQQLSAAAQEHEKQVAAAVAAVMADRAGANVETEALIAQVGALEHLRAELEDLNYSFAASQTELSRLRNENEELEGRCLGLEALTQQRQEQLQQQTQQLHNLQEYTQNLQAQLADASAASRSLAEAQGTVGQLRRQLEETVMQAAVASQRAQQLQMALEEQRARAGGLEQQLAVSHGQVQQLQQQLNHAAASSQAASCPSPQEVEARVEAHGQLQQQNNVASVERLRNAATAAAPGGEGASGAAAVAELRQQVMELQSERAVLLSRANQVDSLQVEVDSLRNARVQLEMALRAEQRRNQAAGGIVLPPGLGGPDGVGASSPERTAGAAADKKRDDMASQLAGMYDPENAVLQGASTTFQPISGLLRGNCPPFASSALGAAARLYDKVTVALYCRPTARLLFSTYILLLHVLVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLF9</accession>
    <name>A0ABQ5SLF9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015841</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="32507" checksum="3AEFEFC55F9AD345" modified="2025-10-08" version="1">MNAVTWGKRRCFLGLVIKLDRETHTLTVSQEHMIHELIERYGMVNADPRSIPMSPGTILMRAADGEVLDCSRYPYIELVGSLMYITNCTRPDIAQAVGVLTRHMSAPTEQHWRAAKAVVHFLLATPQCGIVFGGERVEEGLIGYTDSDNAGCIESRKSTGGYVFTLHGGSVSWSSRLQRTVTSSTMEAEYVAASEATKEALWLRLLLSELGYSLRPTTINCDSQSAIKITKNPVISGKSKHIAVRYHMVREQVACGAVVMEDCRSDDMVADILTKPLPLEKFAKHQKSMGVCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLI6</accession>
    <name>A0ABQ5SLI6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="631"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="511"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="908" mass="95383" checksum="A88DBB16BE9D7E75" modified="2025-10-08" version="1" fragment="single">IASTSDWLQLRTLLSVAREANHLDSRIVAAVFKQAAGLFAEVAGCGSQLECSGRLSVGSLDRSRGVIGTSVGGGGGGGGGGGTVDAGGSPLITRSPPVVTTMFGPAEVEAYASFCNGLTAAAMRALRRRRASHREKSAGVGAHAGGGDHAGSGHDVAQIAYGMGMLQLGSRTLYDTVLRVSGEQLLALTAGRTAAAATTAAAAAVLARGGSTASGRASRKERAAATATFVNGGGAASTETAGLLQSRQHSNSMAAQPPPPPKQWTPSDLADLAWGIALSYGGSSAAEAASKATIDTSAAEGGEAAPTSSLPIPDKAWLRALCDASIDSLTTCAPTLMCRLLWSMARLSYVPREEWMRLFLERVRNSLADFESQELCRIAWSLGAMEVVPDKLWMRALVGQLHNRSRDLNPDQLVSLYGALTRLGYAPRPEVGSGFHAAAVRLMPLMSGSQLAALAYYAACFDRWRPSASFLAALARAARPHAASLSPAEASRLLWAWSRSAPQKQQVLAASPSQQELSSQQQPQLQPHLQPRQQGAEGEMLPPKFMDVLLSRVLYGMRTGTTDGNDLSMTLWALAVLRRLPGTEWMAAWWRAAADPRVAASFDDTCVAQSLWAAAQLRMLAGPVEEASVYGTIDRDGGDGGDGESSQPPPQAAAAAAVATMLSRMSGLLRRTSTANLSTTIAALADLQYRPSDAWMTLFAAEARKRLGSETAVNEDHGLMAYGLAVLGWPLEEPWVRELAAGGYRRMTGMSGEALGLLLWGLSQYGWSTDSKRFWDTVFRETGAKWDSCSARSVVLLYCAVADMLPPNTEPPLQWQRQLARSLRLRVPRPPAAALIPAALRAAAGGCLGPSLLCLRGAAAVAASTPGGLHVNLSPAAAAGPSGGQRTRRSALDESLWSSLVYGANLYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLT3</accession>
    <name>A0ABQ5SLT3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">folate gamma-glutamyl hydrolase</fullName>
            <ecNumber evidence="3 7">3.4.19.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_015148</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + (n-1) H2O = (6S)-5,6,7,8-tetrahydrofolate + (n-1) L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:56784"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="EC" id="3.4.19.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.9" evidence="3 7"/>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015527">
        <property type="entry name" value="Pept_C26_g-glut_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315:SF0">
        <property type="entry name" value="FOLATE GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11315">
        <property type="entry name" value="PROTEASE FAMILY C26 GAMMA-GLUTAMYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51275">
        <property type="entry name" value="PEPTIDASE_C26_GGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046893761" description="folate gamma-glutamyl hydrolase" evidence="8">
        <location>
            <begin position="25"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="351" mass="38634" checksum="C8BE2CADA3FD1F3E" modified="2025-10-08" version="1">MVMPTAKVLAAVVALLLLPTVAYGIRPAVQFAQTAVTTAAVGRPVRPVNDANLRPIIGILSQPGDPAPDGQSYIAASYVKWLESAGARVVPIFYDMDIEDIERRFAVINGLLLPGGGATLAPGHRFYDTARRLVELALKANDNGDYFPVHGTCLGMETLAVIMSSNYTLLGTFDAEDAPAPLLYTDEASASHLLRSLPPDVVADLQNKPIAMENHMSGLSMAAFLENPALGRFFRVVSLSLDKSGAAYISTLEGRNYPFTATQWHPEKNAFEWTPHLRIPHSTDAIRMSQEVANFFVAEARRNLHKAKNILEEDAVLIYNFKPVFTGKHEYVGEEKDFEQSYFFERISHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM88</accession>
    <name>A0ABQ5SM88_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_015813</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10289">
        <property type="entry name" value="GST_C_AaRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1160.10">
        <property type="entry name" value="Glutamyl-trna Synthetase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.800.10">
        <property type="entry name" value="Glutamyl-tRNA Synthetase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020061">
        <property type="entry name" value="Glu_tRNA_lig_a-bdl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="13">
        <location>
            <begin position="96"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI70779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="807" mass="89418" checksum="648C077F91C42D54" modified="2025-10-08" version="1" fragment="single">HGSDCMHCNVIELRFLLPFRRLWVLNQRAGYQLLPGAQTHSLRRLSSMAAAALTFWDRSPPYAALAVARLGEVPLSATPDPKATKETVPSLSVAGESFTGSMILKYLARASKIRDELYCSDALASCQVDQWLETAAGIVSGAALESQCQALNDYLTLRTFLVGYGLTVADLAVWGQLQGSPLWKKIRTSGKVPHLARWFTFCSEVPQVKAAVDELEALGGKKGGAAGGGAGSALGKAAGTMGGADARKAVGGVIADCTVAGGAADSGGSFDIGLPGAEEGKVVTRFPPEPSGYLHIGHAKAALLNQYFADMYKGKLLVRFDDTNPSKEKDEFVENILKDIADLGLRYDRLTYTSDYFPQLLELGEKMIRAGMMYADDTPVEQMRDERLKSIESACRNRTVDENLRMWEEMKAGTEVGLANAMRFKIDMKSVNGTMRDPVAFRCNLAHHWRTGQKYKLYPTYDCACPFVDAVEGVTHALRTSEYRDREEQYYWILAAYQKVWPGLPNVHIWDYSRLNFVNTVLSKRKLTWFVETGRVESWNDPRMPTVQGILRRGMRIEALKEFILSQGASKNITFQEWDKIWTINKKLIDPICPRHTSIEVDGKVLLTLDDGPSEPEVVVVPRHKKYPPAGKKALTRANQIWLEQVDASALVEGEEVTLMDWGNAIIKSITKDPSTGVVTALSGKLNLDGDVKKTRLKLTWLAAVPQCEVVPLQLVDFDYLITKRKVEEDDNFVDLVNPTTRFEKTAVGDPNMRTLQKGDVLQLERKGYYIVDEPYGVKGASRPIVLFCIPDGRAKNMTKTASVPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMI4</accession>
    <name>A0ABQ5SMI4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mce/MlaD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003399">
        <property type="entry name" value="Mce/MlaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039342">
        <property type="entry name" value="TGD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34675">
        <property type="entry name" value="PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34675:SF1">
        <property type="entry name" value="PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02470">
        <property type="entry name" value="MlaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Mce/MlaD" evidence="3">
        <location>
            <begin position="164"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="359" mass="36885" checksum="90931655DF47DB11" modified="2025-10-08" version="1">MSSADTRPVPTSGRCPPAHRIRCPSSLAAHGLGSYLHQLPFRQLPYTRRRLSPALAYSGHSGVDIGATPGSAASSLNSIETNGLVAGSSFNSSAASESSPGGAGNANSNGGMSSSTITNSTRSSFRNVLKRAYLGELLFVVIGSAICGALAYWTIGTPLRRFNPYTFYIMLPQATGVHLGTPLRMKGVPMGSVLSATPMLDRVKVEVEVNEAKTIIPRNSNFELTQSGLIPVPSIDITTPEDVSADVLAATVAARIAAERAGAIDAAGNGATPVAVSSGSSGSAGRAKRSPKVRLAGPKDVAACRLQDVLVCHGDVVDGLQGGSMDELMGHMLKSLRQGENEAPNGAGIVPGRGPLQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMX5</accession>
    <name>A0ABQ5SMX5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_016098</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="269" mass="28809" checksum="0D0841008FF54495" modified="2025-10-08" version="1" fragment="single">TLCLFVTGTDRAVTMAFAVTFSRKALQVSAKATGKKGTSKTAAKPAPSGIEWYGPNRAKWLGPYSENATPAYLTGEFPGDYGWDTAGLSADPETFKRYRELELIHARWAMLGALGCLTPELLSKNGVQFGEAVWFKAGAQIFQEGGLDYLGNPSLVHAQNIVATLAFQVILMGLVEGYRVNGGPAGEGLDPLYPGESFDPLGLADDPDTFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPVENLNDHLANPAVNNAFAFATKFTPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN57</accession>
    <name>A0ABQ5SN57_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycerophosphocholine acyltransferase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="419"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="56199" checksum="A276976DD20A1B3E" modified="2025-10-08" version="1" fragment="single">MEQFAHPLTLRFYDCALERAYQSYRLVAFFRELDTYYLAGQTILAASFFLLQPYLFPSISFHWTNIFYPVVCFVNRLIIHVLPRHAYNCHRHIIQLLARAVVDWSVIRGLPSWSPPVPVRSLGTFATHLLITSGVACLNWASIGWPLLLQYHLPYNLLTATAFIHTLGPHVCGVLYQNARGANIAAVMWKIVRSIASVSAHLRRILLASAGPLPGPAYPSSSSSSSSSSSSSAFQEASNSLWTSNAITDAATDVSVSEPAAASVAVPFLDSLPADAACLASISMVQLLLGFVLPTCLVYILERGSREHFLRKGYRLAEGRSSSVTISKSNLRRSDSESDSDSRRHSNDGGSTSGSALVEVREISGTVYGNVSAGGVRLRRREQHSKPDPSKLWQQQREGQEGKQQTPPGQTGQHGQGRTGTQAGDATPAAVARPPLPPPIPQISPQTLQRLHYYQQQQDFTDGWDPCGALQRGVGLLLTSLLVLMVWELQLYVRLLGPMLLPGAGMRKTTGPDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNM0</accession>
    <name>A0ABQ5SNM0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_016286</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="421"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="471"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="512"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="575"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="606"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="644"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="676" mass="72707" checksum="08F255C7F1CAE780" modified="2025-10-08" version="1">MSTTACPEVTNLFNFPQYAGKCSFFQHGGSTALPQGLGWFILVGLGAIFALVPTFLIWFERKFGSASRDSENFSTAGRSIKAGLTACDIVAKWTWAATLLQSSNVAFKFGISGPFWYAAGATVQVLLFAILAVEIKRKAPTIHTFLEIIRCRWGTTAHIIFTVYGFLTNIIVTAMLILGGAAVMEALSGVSVYAASFLIPAGVVIYTAVGGLKGTVVAEWLNVCIIYLALLIFMFQVYATHKDLGSISKVYQSLRIVEASHPVADNMSGSYVTMFSKSGIIFGIINIIGNFGTVFVDQSYWQGAIAAKPSATYRGYLLGGLCWFAIPFTFATTMGLGARALDLPLTLAEANQGLVPAALAVHLMGKGGAFLMTFQLFMAVTATACAEQMAVATIVAYDIYKPYINRNADGRSMILLQRIMVVVYAIISGVVSVILLKLNVSLGWVYLFMGIVIGSAVFPIAASLTWAKCSSVAACVSAVVTTPLAIMTWLIAAAKLNDGKLNLDTTGQDYPMLAGNLVALFFSMILCIILSYIFPQDFDWAELRNIPVVESDPNSDPNNFEGEDSPEALNYVIKFTWATGGTLTLLLLIIWPLLTLPAKVFNQSYFTFWVVLAMIWGIVASVICIVLPIVEASDVILGAFSGKKPRTTKAAASAEESVQGGKDSHLELPPAPPSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP38</accession>
    <name>A0ABQ5SP38_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016302</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="2">
        <location>
            <begin position="151"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="781"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="874" mass="90543" checksum="D0DF3190BC6C92F5" modified="2025-10-08" version="1" fragment="single">MSTIWLRQPQLQQLLSSARPRGAARGQQALLLPIVLALFFSLLKVAHAGWGTLPPPSSAPITAWNRTIQGTLLYRTTDPPTWAVYFDVLDRTFQLPQQPTASDTGEPITPGNYISLQCFMANLASNRCLGTGNVRIITTAAPVPAADVTLKVLVMVMNLSSSSACKAQGGLTTAEVRNLYLGKNGYADFFQSCSYGKMVFDTNALKVVSVVAPCSTAILGCDHDAIATAAKKALPAGIQIAAYDHVSFVLPLGLSCKWIGLGEVPGVQTWFIPDASGIYRQGTVMQEMLHNFGLYHGWRNGMEYGDYSTSMGFGESCPSAPELWRLGWATPTAVLDADSLPPRTYRTFTIPATSYSPNGPMVKILPTWLGIDYNKNLYFALRLAKGGDVELQQQFARRLNIHEAIAAIDNNFGSFGDPILSLIGLVPVNSNLTLFQYKLQIVTGGITNMDYITFKVCRFISSANECVDVSPPPPPRPPPSPPPPSPPPPSPPPPSPPPPPSPPPPSPPPPPPPSPPPPSPPPPPPPSPSPPLPPPPSPPPPSPPPPPPPSPPRPSPPPPPPPSPPPPTPPSPPPPPPPSPPPSPPPSPPPSPPPSPPPPSPPPPKPPSPPPPPPPSPAPSPPPPSPPPSPPPSPPPSPPPSPPPSPPPPSPPPPKPPSPPPPPPPSPPPSPPPSPPPLPPPSPPPPSPPPPKPPSPPPPPPPSPAPPPSPYPPQPPSPSPPPPPSPFPPAPPSPPPPFPPPSPPPAPQPPMPPLPPPPSPPPLPPPPSPFPQQAPNTNLLPSPPLPGPPSPLPPPSPTPPPPSPRPPLPPSPPLIPPPSPPLPRPPSPPPATPPSPPPSPPLSPPPSPAPPSPPSPRPPLTPPPPSPPPPLPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQK8</accession>
    <name>A0ABQ5SQK8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ANK_REP_REGION domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_017072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="3"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="393"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="610" mass="62827" checksum="C45A9762030BAF65" modified="2025-10-08" version="1" fragment="single">DVHGSTALHYAARRGCSKCLAMLLEAVAPVVVSTAATAATLTAPYSPAEGGTTFTTAVLCRTLLCARNHGGRDVLAEARLSGDQATVQVVESWLRAPPVGVQSTEPPTTNAFTNKQRISSVKACVSTVAANHSRGSSHRARGSDGGCDSSDGGDRKNAVTPAASDSSYPESRSRSRSFSALDSKAVEVAASHGGSGNRDSFQADNGTDVGGESNGGDGGGGDGGQEHVVPPAAAAAATAIEAAVAAVAWDTEEEDAAAVRGFGDGGSGGSLRASTISDMSPQQLEPQDRQQPATTKQPAMKGAANQLDWKNVVVRRVKKSSASRHAGRASPPSAPKSFPLTCAHGRMQPPTSSSSSSSSSGGGGGGGGSEAPRRHHRKDASTQPGLVSELRSSRNSLENVSPQHSGTGRGERLPALPANSGARAQPPAENSATYLSTENPSVGTMTMTISTEQRQGGRVRRSGGPSPGRALGADRGQQLVLQGEEEREKKEGPAALTLTHLVDGVSDVSEPPATTAAATATTAWEMEALRLMQAALPAGSDLELHPSHLCGLGVSELSAAQLEGLDTLHRAALARISDARVALAVRVELERAEEDRQRYLEIRAIRDRDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RL86</accession>
    <name>A0ABQ5RL86_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="193" mass="21591" checksum="B8F49D32747E03BE" modified="2025-10-08" version="1">MLTGIFNLARTCFEVFYPRTLFFASTAVHLQPKVLWWRPRLLSKQQTSYCVMGSQTVVPMEVPVESNVYEAENEMQLSENGNESDVVDCEVPVFQKWDAQSVNSDIDHGVPVVDIRNNGNDTKEDNYERLMAKIPLHALLLTLGGGFASRAAVTRWDMLSLACAMSSGQRRGLSRRSGFGRDALIVPVCAMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLH9</accession>
    <name>A0ABQ5RLH9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA damage-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000226</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="6">
        <location>
            <begin position="14"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="7">
        <location>
            <begin position="444"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="5">
        <location>
            <begin position="852"/>
            <end position="1178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI58495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1211" mass="132310" checksum="E90AB9EC8747536A" modified="2025-10-08" version="1">MFLYSLTLSRASGIQSAVYGNFSAPKAQEVVISRGKILELLRPNENGKMQTVVATEVFGCIRSMAAVRLTGSNLDHLVVGSDSGRIILLKFNKDKSIWVKVHQETFGRSGCRRIVPGQYIAVDPKGRACMIGAVEKQKFVYVLNRDAAANLTISSPLEAHKSHHVTFSVCGMDCGFDNPIFAAIELDYSEADQDPTGEAASMAQKHLTFYEMDLGLNNVVRKWTGAIDNGANLLIAVPGGADGPGGVLVCAENFIIYKNQDHEEVRAVIPRRSDLPGDRGVLIISYATHKKKAYSFFLVQSEYGDIYKVTLAYEGELVTELKIKYFDTIPPCTSIAVLKTGFLFAASEYGNHALYQFVGTGEDDEDMESSSATLVQTDEGFQPVFFEPRPLKNLLLIDEMASLMPITDMKVANLLNEEIPQVYALCGRGPRASLSVLRPGLAVTELAVSPLPGAPTAVWTVRRNATDEFDAFIVVSFANATLVFSIGEEVKETNESGFLGTVPTLHTQLLADNSMLQVYPGGLRHIKPDRRINEWKVPGRRNIKAAASNEKQVAIALQGGEVTVFELEVGTGLLVEAEKRDLGEDVSCLEIPPVPEGLVRSSFLAVGTYDQTVRVLSLDPGQGLKNLSMQALNSVPESILMLYNSNPSSERAIEGGLFLHVGLQNGILIRSEVDRVSGQLTDSRTRFLGTKPPKLFSAAVRGTRCMLALSSRPWLGYNDQGRFNLSPLSYEALDYASGFASDQCPEGFVAVVKSTLRILAVENVGDAFNTQACRLRYTPRRLLIHPETKLLLIAESDHAAIPLADREDLQAKLAALSEEEGGPMQGVEFNDELAALEEQFGAPKGQPGQWAGCLRLVDPATLSTVFVTEIDNNEALVSMALVDLAPPGAQGGGGTEKLLVVGCAKALRYMPTDCEAAYIRVYRLVDGGKRLELVHKTAVDGGVPGALCGFKGRLLAGVGPTLRLYDMGKKKLLRKCEYNRLPHQIMNITVQGPRIYVGDAQESVHMMRYKKADNAFYIFADDIAPRYLTSLLPLDYDTVAAGDKFGNVVILRLPREASQQVEDDPTGGKMAAASGRLNGAPHKLEEVVKFHIGDTITSLQRAEMQAGGQEVLLYSTVMGAIGVLYPFTNREDVDFFGHLEMHLRQEHPPLCGRDHLAFRSSYFPVRNCVDGDLCGQYASIPAKKQQMIAEAMDRTPGEMLKKLEDIRNKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM47</accession>
    <name>A0ABQ5RM47_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_000402</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sterol desaturase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="8">
        <location>
            <begin position="117"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="268"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI58660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="302" mass="34513" checksum="5D5715D0DFF8261F" modified="2025-10-08" version="1">MDSSALWTQAVHVFSDRLRSGNPEFGLVFYLPIAYWLAAGFYELLDWMRLPFTEKYKLNRREPGRGNMITHQHVILRVLLQQVIQTALTLAVMILDPDMCGRCADSTKNPIQRISRFILGMYVMDTWQYWIHRIMHTSTFLYKYLHSVHHSLMIPYASGALYNSILEGLLLDSLGGVVTHYVAGLDCDTATYLYIFANIKTVADHSNYRGPVNPLHGLFPNCAAYHDIHHDVRGIKMNFSQPFFTHWDWLLSTFLDPAGMHFTVEEQAKKQEGRLHKQAAQEPPGLSNPVTTGKGAESKKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM56</accession>
    <name>A0ABQ5RM56_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000002">
        <property type="protein sequence ID" value="GLI58388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="297" mass="34149" checksum="BDCBCBFDCEE55304" modified="2025-10-08" version="1" fragment="single">MSMEQQQSMQSSGFEIVSALMNVSRVEDLSVVTATDVVRRDQSVIALRPLHAIGNVKEDEMAFALAEAAKAELAYGGMELQQITNVQQQQQQQDIQLKQLEQQLNERDQQLQQQVDVKLQNVAQQQDGKLLEIMRQFTRQQDEKMRQFTQQQDEKMQDFTRQQDEKMRKFMVQQDEKMQDFARQLLDEKMRQFMQQAEVIRENQVARSYNVFAIDADHTLEPLLVESVQAQHALGTAPSAVPVNFPATVDAMSKLTNHDLDQLQDFYNTKFSGKSIKTRRCRFAVYIGATGVIRQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM60</accession>
    <name>A0ABQ5RM60_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000425</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00990">
        <property type="entry name" value="cpPDZ_AtDEGP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051201">
        <property type="entry name" value="Chloro_Bact_Ser_Proteases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039382">
        <property type="entry name" value="DEGP1/8_PDZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43343:SF2">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43343">
        <property type="entry name" value="PEPTIDASE S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="461"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI58682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="588" mass="62428" checksum="87EF1B2111F86C8D" modified="2025-10-08" version="1" fragment="single">GMKSTFLKRICGHLCSSPSCIVSIHTQVTDDLVATPTIGLLAWTFERINAKVLLKPDRLPAFLQIEEHSSVKRFGRALVATIMLQKRDRVWSPSPSLRRTAPSMSLLRNSSPLLMAHCRALTPWSCAGWRSLTDPHSLTSVCAYSHTRSANQELRSEGLRISGSAVLLAAGAVLSVLLGSGVGFTGGLLAPPMARADPALEKAGSGIETEGRGATASPPRDVQLQRNLDPMTMQLPETPPYLTPEEVRAIRIFARNTPSVVNVTNIQQIAIQNGYWSLDVQKIPAGFGSGFVWDDKGHIVTNFHVIRDADEVKVTLLDQSTYSARVVGGDADKDVAVLQLQGVAPEKLLQLHPVTLGSSAGLLVGQRVFAIGNPLGLEHTLTSGIVSGLNRELSTGTFTIKGLVQTDAAINPGNSGGVLLDSAGRVIGINTAIADPSGKGASSGVGFALPIDSVKGLVEQILAYGRVLRPVLGVTLAPPQVLKQLGQPGVLILEVPRGSPAEKAGLKPTTRDRFSGALVLGDIVTAIDGRPVKNYSDLVDILDDKRVGDTVKVDILRSQTDNLFASGAARKMTIRVTLGERGQVNVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN04</accession>
    <name>A0ABQ5RN04_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquinone biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="39917" checksum="E750BD5DC54B2CC5" modified="2025-10-08" version="1" fragment="single">MAFVKRCGGRLLMRLSRCVPAESAACSTSLPLQPHFMAFSSLWNSTGVRRMSGGEEPSSRSAASVSPNPAGPQPPPPGTAPSRNPDPQPGASTSPAPSSPPSASSSSHSSPSPESSRSGSGPADIEFPSAAGKPQSRSDGSSMPTSKPTSKLKAKTKPEQDDDSNRDQSREGDEPENVHKYHAFWRNERVRRMQPQIEEGMRINAYEGLQGFGGFMRGPDQAWSFLYDNPSARLSAAALVPLWSAGVALVRPLVLGPTGSMIRDEVDRSFDAQEFVESVSQAVPVFYDAVATGDAEALSRMCTPRGIEALDRDRRRLRMELGLEITRVGTKVRHATLAGGNLWGPESIRAFDPAWAGDLSPDMSRSWLVVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN41</accession>
    <name>A0ABQ5RN41_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000624</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF0">
        <property type="entry name" value="LD18904P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="72"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI58859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="285" mass="31905" checksum="E9CF045F10EEF33A" modified="2025-10-08" version="1">MSLEQAKLHQVFQFYVIPNLATPGSKPNLIDFVVTIWIALVLLGLRLAFERAVIPRFKGYLERVKGPGGGKAAFPVLDNIWIAFFAGSLAGLAWWVTVTGNGGCTPWSTSACFVGWPHHAVALGQRWFMVLAFAYYLYELFGTLLGLGTKLKIDMLAHHVVTMALILIAYQANLKRMSVMWQALFDISNPILHMAKGLHSAAIQPLEGLKWAMFNLFAVTFFVCRVVMGPISILWPSFTIAVQWLPASHCYTCWGLVVFVYFLQLVWFYKIVEIAVKGDKGEKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN60</accession>
    <name>A0ABQ5RN60_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_000819</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF8">
        <property type="entry name" value="POC1 CENTRIOLAR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="337"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="9">
        <location>
            <begin position="618"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="507"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="693"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="516"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="526"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="540"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="693"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="706"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI58996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="853" mass="87944" checksum="714B8D7BAA242102" modified="2025-10-08" version="1">METLFPEGSCLRYWLSNASVSQIACSVSWPPIIAAIGYAPCVRAWAPTSKVEPEYKPVLQLLEMDGERFVAVTVKGDLLVAADASSNGRISIWDTTTWIRQQTLTAEAPVTSLDASRTYIAAGTAAGYVRVWKRQSASDATFTQFFAPDLQLDSLPVYTVKLGPLRHGGCGAGGGGGSGGGGAGGSGGGDGGDAVMVVHTRGNSRVSAWRLSDAKIIGTLSRSGPPDSPGALLSANVVHSCLAKDVLLTCSWLPGEDPLLQWIHPVQRVGGEVPGISQHLAGLARPLCCDYDGSVIVMGCEDGTVWVWGLLVQGQTPGPAQHQQQQQQQPEEVSHWRGYHRGAVGAVACLPHRSLIASAGADSCVKLWSMTGTLVAVTRLGWQPTALGINELALIVGGAAGQIQILVLLTEERAASSRPTDADQLEARTPPPLATATATSQGSVMPYAYWFDARSSKEAAAAAGSSNGGGSGGAATGMWGLASAYPDAFSAFVPPRRPLALSDLQRVPMGQSDGSSTGGAAGSQGGFSAATDAADAASGSGSGGGGADGGKASSFNMQAALKGAQRNAAAQAAAQAAEGEARRSQLRAMGEGPGVARSGAAVQLESATAVTMGRKCANPSCLQREGLLALQQRHHHNHNHNHNETGGPAAGGFKRCGACKSVFYCSTHCQSTHWRDGHKKECSQLAEARRRQQEQQEQYHREQEEQGQGQNQQQADWGEADSAESAPDLTAGVPEAADVGRDHNGKGGLQSSCDIMGSGSRKVSVLAGEACDGGCNPGLVDEGRACSAAPISTQAPRKAGPPILMKELTTELTPMDVPSAVAAVAAAPPPPLLVGSHGFISGCAAVDCLHELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP24</accession>
    <name>A0ABQ5RP24_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="605"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="612"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="643"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="663"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="748" mass="79027" checksum="15FC9D8E599DC777" modified="2025-10-08" version="1" fragment="single">GSGGSGGGSGGGSGCSGPMGLLSLCVDYSQPSIDRFEAELLDAHLPHLPLLLMELYGAAAAGPVASDAALRHPLLPHPALEAADSGTTTTTATTVGPLLRLTSLELRGVAVQPGLLTELSQLTMLSRLVLSISSVRPEESLVRILPRLTRLTNLHLFLHLHTPAEARQLMAAAAGVAAGGEQQPWQHRGCRLNETGCPNFSAAIQLAAAVSQCTNLVALSLPGEDVGPWLQELPGLTHLAARQLGPWDLAQPHTCLRSLELTMTWPAHLARLLPYLPNLDTLLADVYVYANGDDDGDDDGDGNGDDDGDGDAYKKSKKGSGVRRHNHRCRGGRAVKEWGNGGGKAAAVGGSGAERLYEEMLAVASGLRGVRHVELQLSVMRRERWEWPQLVRVLAGVRGLRGLTLRLPQKSSGPQLRAQHFISLCRTLTGLRRLTLLVAHPDCVKLNAKALQALQELPFIQLVQLHLGGAWEGHRMLQGGKGVDADADAAAPGGRLGGGDGGRLRQTSSNAAAKQRSGDTLHRSRRTGRSGKHDKYGKREGSPDPGMTCQRLGPERAAAAAVSPDRALAGDDEDRGASSVLWPQPIIPNSHPARVVLEVARGLGCGREQQRQPRRRGHRHSRGGSDAGGTTDDSSTDTDSDTDSLASSVASVSGLASHGGNGASSLGTSNDRSGSGIGGSSMSGSTRNSGFAVRTPGEVELQVDFLTAREAAEVNAVLRWERRPARVRVRVRAGLEPRWVWADLVNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP98</accession>
    <name>A0ABQ5RP98_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Integral membrane bound transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_001252</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049453">
        <property type="entry name" value="Memb_transporter_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31086">
        <property type="entry name" value="ALUMINUM-ACTIVATED MALATE TRANSPORTER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13515">
        <property type="entry name" value="FUSC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Integral membrane bound transporter" evidence="7">
        <location>
            <begin position="75"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="493"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="677"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="770"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="870"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="870"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1012" mass="107594" checksum="019E2856D40D39FF" modified="2025-10-08" version="1">MAPIVSHVQSGLHRVSMGLRQHAASIQKIKVDFSKVPDWTRRATRLSIALVFFGILGHCAKFSPEHQVFASNFRQSCNWAAITCIVVTAPLIGKVAQVAFDRLLGTAVGGLIGCVCFTVGFHMFGAFGGGIFISLMSGVAVWTTTYIAYKFSMDQLVRFVQLTYIIVAFGAKPDKGAFLLALLRVGGIFAGGLLSVVLAVVVLPRSASIDCLLEMKKALKALYDLNQEVWAVSGMTGKSMSRRSVRKTHRHGYSALQLRLDAAAASAALKSNASDPAMATAMPPDVRCAGSGRVDRVSSLPDGLRRGLHDSSLNHLNLLVQRDAERDLHEAAIEKLFTAVYTALGRVDEHLAQTRGEIYIWHFWGRYFFLPGVHWFPVTGRWRVPKEDLDNMVMCVRRVARMLWTLLLDFEEGFGSEMEAVLQLYYPKQLLSELAEYQARAILDLLRAFPGASTIEVDNLFTLGLVTDCLMQISDARARNTVFKMKRRRATAAAPSGVASPGPRTGRGTATVSVRGPLQGDGEVVKTADCRRSVELSMTRLQQPGNCVSAPAAGVEIEKQPLLAAIAQSSSAKLAAPNPTAGKDGSGWGESGSGYPFQRLSEVGSGVITGGPGMLHSGLAAVPVGDLTGEPSGGSNAGAAGASGGDSGLMSGMFISRLMSGTPGELMTLHLPPRRYLQEQQQQQQRAQKKQGQLERGLPTTLDDPAQGADAAVVQTQPLTIAQRQGSIDIFGGVHFNTLLGDRFGEAAVLENATSGAAVAAADMQSPVGTLAAAPPDASGHSASSLPPRPRSSTGDPASRSMLPMPASLSPPPQQQQQKGQAYAGHSRTPFDTSTNVPNWMEQLRSSSLALSGSEVAAILRPGSNEPSAAAAATVTGAASTTGLPQAPVPPKTVRTRISPTRTVPQAAAAADVAVATGMGSAGCSTSVRSSSEVVRGTHDRDLERDGIQRLNSALLSVSVEPVTFPPTEEGYLSQVRWYSFQFVMDEMVNELEEAFHACSALLQKLPYPIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPI8</accession>
    <name>A0ABQ5RPI8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_001359</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="1218"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="538"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="645"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="822"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="991"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1079"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="583"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="662"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="825"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="851"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="991"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1001"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI59476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1605" mass="166442" checksum="165449846D2E13B9" modified="2025-10-08" version="1">MFCCFRGLPAAQAERIAVISDFSPSVHNGGPSSTRSAEPGKAAALLELVTSIPSDCATKASLGRINCSGNVDRAVIEYEAGPTESSQQRHGIWASLVESLRANAAKQASAVGSVGAPMECDAALGLPPAGTVMGLPDLLQQMDNLADPVALVTFDRIAKRALRSQRVSPHLLMDINMEAMFAGGVVSPPCLPDTSSEQQSLEPSGHMPAQAVLHLLQRHYSHAEAIRLSIHHTNSALKSLLGCPGVVEYEALLAELLSTDSMLQAVLHDNCLCMLEGLTTSNAQLVVHHPIRNTASRMVLPVMLQPVLVWRRPRGAPASVSEMPTMAVVVRYMLSAGLHDALQHLHRVYTMLSSLSGIVTLFTLRGRVLHQNTASVRYMGLRTGYCPDTLTAANATTGGGGGVADIQQSNDLTDASGLALGSLLRQIFALSSEQLDDMLEDVLEGKTWKGIVRVPSLLTPTTDPGPAQAAVLVGRGMSGMTTGGTPNGSNAQVMSTWERLGPVLMSTQPGARRSVAQNGNQSDVEGHSDMVGALMMTQLDGDKSNGGPPDGAQQVGPRLGSTMNSSAPPAAVAALRTNASTPGGAGSRTGGGTGEISSPDSCIFLAIGSTSGTTFSRMNNGSHLATQTSSVRMAVQQRTIVSRLSSNDLPEGNSLAATLSAPGGGGGGGGGSGGGRGSGGQGSLTLGTSSGGGGVNSNACYVASKLSGSAPVIPSNPRPQSGVRVPGTGLLRTLMENGVSIIGEIDRASNHLGFVESKAGMANLTEELRRRNQHQQQPGLTDAARRSIVRRRSALKMVSSSTDDNPLSTSTWGLFDNHRGRGESKSFTLRRNNSPTASNVKRVGTERQLERTTVTQSQNEFHSAVPTASSLNSGNGSAKKRPPSRLMSFLANKNIAGAPVGGGHPPRSLNPGGGNQLWSTSLVSDVGVTGGAAVTLLKGVSSTTELELGSRQGSSLGRSGLSVLTMQHLTNTATAMEAAVTSTAAGESIGNSGGVNGGGTAANTGNASGAQAASPRRPPTRRVLRPAVEDVAEESGAESSARLPPLSVAARESGCNAVATVGAAPHLSAVMHAARASSSSQATSRVPTAGQLLSPSQVGPAWERESGVADDDDDDDCWHEITASTLRDPVNGEQAILLMQVDVSARVRAERRIAEVLEAEHKLLESVFPRHVLELAATRRPKNSPESPGVGPMSKFSLAELPLAQNCTSVATYHPMVTILFSDIIGFTSMCHEIPATKVMEFLNNLYSRLDTLLDVYGVYKVETIGDCYMVAGGLMTRDSDGFMTVRGSDSTDELHAAKVMSFAKAMLRETSEVLLPTTGEPVQMRIGLHSGPVMSGIVGSKMPRFCLFGDTVNTASRMESTCPPGSIHVSGATREFLLDEDWEPTGGVMVKGKGVMETFRWVPLDPESSPQIRVTGCQRHYRPRRASSIAYIQTGGVGGGHGVGNGVGVGNGVGVGAQSFEGSGNITLALQAVQGVGSGPGGLSSATGSIGINSGANNTHHPSPQGFSATQNGVPVKGCRAASANITFMSAMGFGPQGQEGHQGAPGVDTFVGGQGPQMHFGRCRASGFNCSSLGSANVTGMRRKPHRSCSTTALAYKGLSRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPQ5</accession>
    <name>A0ABQ5RPQ5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000776</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="535"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="592"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="632"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="712"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="468"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="632"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="725"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="738" mass="80965" checksum="B8C155E0E2A4CBCA" modified="2025-10-08" version="1">MHTSMRPLLNSGTSGSIAVHSAAGRSARRLILAPNPCTVPSTCPRSTQVLNPDDELTRKTVGAGSASASADDASTTAILASRGIERSLNNFDAAVLPQDSHILQDDGHFVRPPASQSDEPQPLASTSAPCVVTSSLPKRRRGRRAAAAGTGTLSSTGTSGQAGRAGNDGDSQDSPSHSPRTRDAAAVLASSTAPRPRLESPGTTLKHQARPRLRPPPPPLSWPDYPYIRHPVASACSPSDTTALKLEALFQCVLHYPRLPPKLLRFLHREVPDGPMGRLAEVTATVERLAEAVGREVAMYLIRRSSWLVLVPAESVAPRMEAVRRLLGLQPGGVPDLLRKNPNLLRMEEETLRGRYDALQNALYDAVGFDETKLRTLIVKYPLILNFKHESVHNALMALRRLCSARPDWVTYYKALSPSQVAFFIRERVMTLLRLEYLLLTGGGNGWTLRDVMKMSNNMFRKAYSGFRSWMQERMKRLQQRMAAARQAVEAQAAAQGRQLSREELERVAIQVQTQYARAEQERFHRQFMARREAERQDFDKRMEQRQRSIEEKVQKERDAELLAWQQRHGGQGAEGDEQEDEDGWQEKHRLYQQNQQQQRAGSSTGGGPAGLRTSGSLVAGAMLPAAADAAAAGRAPSSTPYPQSSSGSGSVRGSAGSMTGGPGRGSSARQDARQYEKTAVPLLQRQHPPPMSFSSADPLYRVTLGHVWQRPGSKVAPEPAPGQSLPQYQQWRQGTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPW9</accession>
    <name>A0ABQ5RPW9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF56">
        <property type="entry name" value="TAUD_TFDA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0045">Antibiotic biosynthesis</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="3">
        <location>
            <begin position="66"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="360" mass="40109" checksum="02D1D4214337AA33" modified="2025-10-08" version="1">MDFRPQSSRQGSVVPYTIIEGPDAWTASDFPVLEDHMLHLTPTHVAELDAAVEAVLSSGKRLQDIDFADFTLPTLSDPLLEAGQEAQFGRGWALVRGVPVWRYSRQQQLAAYWILGLHWGRAVPQNAKGHLIGHIKDIGHDPKNPLTRLYATNAAQPWHNDGPADLVSLLCLSDAEEGGDSGWSSSISVHNEILRRAPHLAAVLAGPWFFDRKTEVPEGKKPFFEIPVFNYHKGYLSVNYSDNYYHLSQRHAEVPKLTAEHHEAMRLFNELASGPELSLRMVLRTGDVQLLSNHTCLHYRGAFRDSPAHTRHLLRLWLSPPNDRPLPECYAEIMAGSVAPGKRGGIIIQGATPNIPEEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ01</accession>
    <name>A0ABQ5RQ01_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="42555" checksum="95B76F526775715F" modified="2025-10-08" version="1" fragment="single">DAMEGVEGAGGEAGTPLAVEPTAAPPHDITDLMADSDAEGCHDAVQPLPPPPSPADVIAGVTKGGRGGEEMDMDAERDEGPAAAMAAPNSGARGAEDDDVTEAPEAEDYLAVCEYVRGRSQVSLAHLARQFKSMSQGALAGYLDRLKGQGVLIAVPGARSRFRVITQALATSRGPDGKHIEAESALLEVDAGTGAGGAGDAFTSAAPAAAATAGRMAKQESSQRRPQGHRAEDAAALIVKRLDGLSMDVTHGGAVDMAAAAGGNGKTEVATKSDPHRSRDQTERVDGSRGGRLESAQAGDDLTGMTDAKAASEGKHLAGAAGLQPNLATDDPHVYIVDSQQSGLERLRGGRVRKASYVADPIQQGLVSKKAKTGSTAAGKAVGPSSAAGAPMHSGDKADKAAAPTPRGTRSVSRLSGMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR99</accession>
    <name>A0ABQ5RR99_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="907"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1107"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1126"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1138"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1249"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1279" mass="133339" checksum="82330F8F9BA2DB54" modified="2025-10-08" version="1" fragment="single">MSRQKNSFLVTTGDGNIDGWVAQAQAQAPTFGEPFKFGTGLRFTRKPPSYLTELQLRSLAYLDPAAAAVQTALRNPQNAPVVRARIVRHGSLDGSGFLNAAVAPPQAQPRRRWRHSFHSNSQNMPPSTAAAAAPSAAEMSAAAASLPGTQRVIPAPPPMTATVSRAPVVLPHGRRITKATGLVLREWGSLYPQAWALAGAENGVGVLPPSLRTVTGMGLESVLVVERQDAADWRRASAAAAVPAPTNKPGATARRHKAKGHPAGCRCPACTSLVAMFYSESEDSEDDTPGPGQYDVAMAAGTVGRRGPAFTIRGRTAPPPSATFINPEAAKVPGPGAYNIPRPVTEGPAFTIKGRPRGPSTLPDDLPGPGEYYDPSKTTIREGPAFTLAGKAKRKFETDAPGPGAYLMEGPSKTGPAITIAGRPRSRAPEPTPGPQDYQHGRALSPGREGPSYTIAGRKDLKPDTDVPAPGEYDLPTAWRTGPAVSFAGKPVPKPDPEPSPGPGQYHVPPRPGTGLPAFTFGARPSEPGPGDDLPGPGAYYRPVSTDGPAFTIASRPASPGPGQRDAEPLPGPGTYDPQPGGGAGPSSPSGPAYTFGAKPPERSPPPSPGPGTYRPEEALAAVLPSGFGPAYTLGTRPLLKELPESPGPGDYRPSDPALSPGPAFTMAGRPVERDPRVDMPAPGEYETGIRPSDGPAYTIRGRPMEEKRPVSPGPGEYNSPAPRAGPAYTIASRPSSPGAISPDPNQKFPGPGAYDSQHQSPTGPAYTMRGRPEERSPPPSPGPGDYTRPAGPGGPAFSIRGKPVEAEPPLSFVGPGAYNPEKPLPGGPAFTLQGRPRDHKSPQFPGPGEYDAYGSGANGGPAWSMCGRSGDQRVADGPAPGSYNLPASPRGPAYTMRSHAPEPSPERSGPGPGEYSAPSSPTGPAFSFRSRPMPQETPESPGPGDYQQLAAGHSGPAFSIKGRNDNGPSADPDIPGPGTYNNPDPEALGRSGPAYTFRSRLASRMEEGPGPGEYDSPGSPAGPAYTLAGRPASPGAPEIPGPGAYSPTRRPDGPAFSIAPKVNAPESEPTPAPGDYGTADRGLAGPAWSLSQRLPTEPLINGPGPGEYYSPPSASGPAFTIGSKLKNRRRRKRSQVPPEPGQYWSPPPPSGPAWTIGARSPGPTPYQPEAGPGDYDDRLDMHTGPAFTLRPRTSSSPPDPVPGPGTYNDAKPFPNPLGMVISRPRAHTERDPVYITDMGGPYDDTMKPRRSPGRPSRKVTFHQDTLFRDSMEPTYNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRF5</accession>
    <name>A0ABQ5RRF5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="147" mass="15050" checksum="2FF232BA3C6D4FF2" modified="2025-10-08" version="1" fragment="single">DGSKESSGNGNGNGNGNGSGSGSHVNDMRDGLTAEDGKTTGSRVPGSAFIALAADTGTPGGIGGCRSGISRGGDDEGGGCSGPGDGRDADVEEDRLLAIYDALWDVVRAVERWQVGVHRWQVKVWKQQIAMRAQTLAMIAAFTGISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRG0</accession>
    <name>A0ABQ5RRG0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="373" mass="36205" checksum="79F787F027D55EFF" modified="2025-10-08" version="1" fragment="single">GGSRGRGGEFRPLAHRGGKTYYHHEEPAQGAGTARQDDVAALNDGAGQTEFGGGGGGRRGGGGGGRRGGGERGGGGERGGGRFGRGPRGGGRFTGRFSSSGTPDGDPSAAAQLPQVQVQPPDSAAALAPVLAPGPSVPGASTGAGRRHSRHEPASPHAPAAAAVLLGDGPGEATSTATAAVAGERLSRSGGRGAGGRGTGRGGRIDGGRAGSTGNGNARGEGGGGRHGGRHAYEPQHHPHPQHHGPEHGLAHVHVHGRGRGASGGGAATEAAGASSDSRSHPGRTSGGGAAPVGAPSGPSTAVGPAAPTGGGRKGKADSTQGLHGPHGVKKDAPEPPHESKGQALFKKYADAQGSKPTKTSNVFDLLNMMGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRR1</accession>
    <name>A0ABQ5RRR1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002364</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sel-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF147">
        <property type="entry name" value="SEL1L ADAPTOR SUBUNIT OF ERAD E3 UBIQUITIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045356901" evidence="4">
        <location>
            <begin position="20"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1810"/>
            <end position="1826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="300"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="829"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="993"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1438"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1527"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1590"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="465"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="506"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="581"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="628"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="668"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="702"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="738"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="853"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1001"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1590"/>
            <end position="1602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1835" mass="196648" checksum="AB46908581803569" modified="2025-10-08" version="1">MKRTTCVGYILALLVCVGGQQDGSQWQDKTRQYYHECEHYQQQQEQHQDIVAVCGNPTGTYQQQSLAQRQWVQEEGMSNANSQEDVAYVSYVTENQSTQPREQERYVDAEDAGQGYLVQPESDQARMAMDNIAGNQYELYMQGMGQLHQELQQVDLHHQGPYLQQQYSVDEAGGQCDTSDRDVPKVECPQEVINHSQQAEEHANQATHPSAKMHDYIVYNEIAALNDYADSDRPKSSDNSDVHSPAATRSEAKPLVDQFIEHSSPPASGSNAQTIDAQLSPPLKQSVSIHEWPSVPVAPAEEQDQQVDSQQAQTQSSDLEQQQQQSIYVHEWPSAPVAPAEEQDQQVDSQQAQIQSSDLEQQRQSMYVHDWPSVPVAPAEEQAQQVDSQQAQTQSSDLEQQHQQSVSVHEWPSVPVAPAEEQDQQVDSQQAQTQSSDLEQQQQQSVSVHEWPSFPVAPAEEQDQQVDSQQAQIQSSDLEQQQHQHSVSVHEWPSAAVAPAEEQDQQVDSQAQIQSSDLQQQQQQQQSVSVHEWPPVPVAPAEEHDQQVDSQQAQTQSSDLEQQQQQSVSVHEAPSVPVAPAEEQAQQVDSQQAQIQSSDLEQQQHQQSVSVHEWPSAPVAPAEEQDQQVDSQQAQIQSSDLQQQRQSMYLHEWPSVPVAPAEEHDQQVDSQQAQTQSSDLEQQQQQQSVSVHEAPSVPVAPAEEQAQQVDSQQAQIQSSDLEQQQQQQSVSVHEWPSAPVAPAEEQAQQVDSQQAQIQSGDLGQPKGPVGMVMESAWVTIADEGNSAHKEAIAAAAEVAVVTEASSGVQPLYTIDPAIQLQESLVSDQEDALLTSSEEPLPLTAAEAVSDVGPARDRRLEGGRPDSNDDANARVSDTDAAAAALTEVAQAEASRGPLHFKPATVVPRGDGTFEAASETDRVAAQAAAEAASLHPLRGIKKADIENKLLEAQGLIYANTSCRNMTAGLIKLRELHEIVEAALPSSPMIRARLHSPAADDKNSSSAENQTTTAEISNAQPPRPLALEVAESRHAGLHTAAAATFQLAALHSSGVVPPDIMPYDPRSAILALHQAARAGSLEALMALADRYAQGIGTPASCSRGLQFGKLAAMYLVTDVEKEQRYAPSLAPVSLRERFADGSYVAAEDAENGQHVINLEEDMAFRGNPDAIRRMAYRRLVGRGMEVDPEGAFHDFQVAAAQGDPYAVFNIGYMYLRGLYVPQNFTAAKEQFEKAAQKGLPSAHNGLGVLAWNGQGMAPNLTAARESFERGALLNNSDSVYNLATMHFHGAGTPINQTLAMDLFKRALELGHWRAPYMMALAYEAGTGVEANCSRAMQYLRLLFADRSTWGKQLTAAVRLLDSGDNRGALLTYITVAEQGSSAAAANAAWLLRRRLGYSGPDADRLAAKYFQRAAKQGHTSSMVELAHMIMEAAMRVAAPASVPAPAGQPTSITAPVEGTGLAGTEKTEGEPVVGGPTEAIPLSTVASSSGAEGNPSGAAADLRVSTETVDGVSTMDAAVAAEAMQGVGDRRAATHEVSTDGAAITGNTAATSGPLADTIMRDTVATPAGAGDADGGSGDGSVAVTAQSAAANASSANSSGTNSSTPKALFNLPPLKSSTKRPPPPPLVLPAALPTHRVVLGVSKAADAVAWYSAAAATGDPEGLFYLGWVYYHGLGVTENATFAKLLWMRSLEASGIRSTRALAPLLALAGMRIDGWLAPLVGHHALARGIAQLQRGQYAMRALVSLSGTGGPGRPTVQGDTGADTGAENGAIGAGYGLVVRAQMRSILTVVKAWLGQLVAASGDVELGNLENAMLLGLLAALLVVVVLRRQRLAVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS08</accession>
    <name>A0ABQ5RS08_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002162</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="457"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="876"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="897"/>
            <end position="906"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1044" mass="114187" checksum="25FC05DACCFEDFB0" modified="2025-10-08" version="1">MVSWGLLATTGLSVLLWIVYCFWKSFAVKIINLHLLVKYGMAETAQRFTDGGHGVVKASFLRYGRVYFINGWFEAQKVFGSSAFHARLGDIEGLRLLGMDGMGIIWNNDDPKWRIQRKIFTEAVQDRRDTKRREVARLCHDAVQLTLQEARNGGVATNRDDNVVQMNGLNLLRCITCRVTLALAFGLQLGSVSYIESMGLVRKVNAFFKAWEFFLLRPWWYLWVFRRREALQHRKSVLRLGRAVEDVLIQAAAGGGCGFGGVTTATAAAKIATGTGAATTTAPFLKSIFEHLADGRLSRAEAVQLSLEMIIAGTDTSSVTLFYSLLAIRDDQELQRELWSELRQKTEGPCYELRDLRLLQAVLSESMRYKPVGPVVIRQATKDLSLERLPVQGRGAGEGEEQGLQQQRQRRLPHRLRKGDTVVVNLAHMHRDPTIFPYPNNFDVRNFLDPPPPPSIVYGSSGEPMPTRDVTTGPGTRRSFLPFGDGQKGCVGKLLAMDEMTAIMWSLARSLELPALPSPRTPSQPPPRQRKAALLAPRLADLETKWEVANQPKEEMLIDIRFTSTSSPPMGPSTPERLPAPLQRAFPVSALDGGLPMAPPPPLVAPPDADTDTDATIDVVVGADPLPKAGNTNITGITPLPPLMDWSRQRRLQYTTSAAVPAPPGCWRRVMVYLTGPGGVGKTTVLRDVETSLLAGLGCTTRTEEARELLKILGIGQSQLEDDAAVFLKFQKELVKQHYRRDAPAIRRLVTTGVEDDAAEAVAVTAAAMGAVASAKAALSPAEDATVAMGAGAKVELPEERAAAEADAVADAEVAEVEEGDSALLLCDRSAIDALCYTAFRFGIDSPEVEEVLGMTEMKHLLELYGARRVPTSMSLPASTDAHLPPAPLPTPEKARTTNPADANPSISVRIPPSGVASAVSIHCLHVLVPPFQPSLEQYDAKDGQQQRHDQWGQQKNGVMDDDGTTGTEVADGKQAGGTAAIAVEDDGVRLRVSLDQLEKHTAIAAMLLQRLQVPHLVLQERGQEARCRELHQILEDYFNILYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSB3</accession>
    <name>A0ABQ5RSB3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="725"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="948"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1215"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1315"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="733"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="746"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="968"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1334" mass="134467" checksum="7311FD283199B2B9" modified="2025-10-08" version="1" fragment="single">IAHHTADPVFNWQFDLRLPADLFSGSDGAAAAAEPTLLYFRVLNARTLGEPEILSHAVMDANMLGLKINEEVRPKLLELTAGAAGGKGGGWTRGTLSLQISWLKALPKRASPFPQDAGRTAAVPSPLPPTQSHHSHSNSVATSTAAAAAASPAAVQSGSYQSQTAAWAAAAASAAAASAHGGSADGGGASAGANAATITQRRTELFAPSGVAGAFDMLTARMQDVAGSAPMYGIGGGGSGSFMADEGSFGVSRFGMGSSRSLGPMQNTPHLPLEESLAGGKAHNSKTMPEARVVRHGPHDASLDSGSWQSLTTDTVLPHLNGGGGRGNGAGKNNHATTNVGSNSSRGDVAAPATAPLAGITTPRRSMDTTTEGGSASGLASSSASGPVPMVFRRLRKTAFRKSGGSSGGGGGGGSGAPGSPGGAVMQSAAVERKAAEQPGPPGKHRRAIRGLFGFGSWKGRSSHVRESAPAAAAAVVAAVVAAAATQLPPSARLSLTEAHDDAHSLIRASGDSQDTEIKGEQGFCDTAAAAAAELTTDGVDASGVAANAAAASTAATGVSRGSFEGLSLEPRGFGSPFSAEICSKSGTPRTTAASGGAASASPQMNGVAAMVTPPPLLPPRLKLNFSPSPPRVDQDRKANACSTAASYLARTSVDTRLRAATAATAATTAAAVILGDGLDSLVFDAGALGAAAAAAAAAGEEELSASVSEHPGQSHRMQPDVVGLTEGLSKSTPPPHTPPLPPRASISLSPLPDLEELPSPQRRQQRHHLQEQWEQLSLQHVEQKGQGQGHGHGQVDKSGDGCASQQLPDSPLAQELQQRQESAAAAMPPDVVDPTRRLSICLSAPSAIKKAHASGPVAAAIAADSGGSGDGDGDSIAAMAAAHGPLNPSSIPTATACVQTAVASLPPVLQQGQQNDSGKQPQLQLLTLSGSRGSAFATPGAAAAATAAANGTSEAQPHAPWSHTRTSNGGAAAASSMSRELNRQLSAISVVSAAAADPGTAAMVSSGPLTAAPPALSAGPLGTSPPPWSSTLQSLAQVLKMQKSLQVERQARAELTEMLSDMTRKYEGMVRIRRFENYRALVEGARFQLHLANEMRRVLMWYNEARDVVQVDPDVGFGSDRSQERTVGLWPRTFAPQELERAERGCAGFPSPNPGWAGVVAALITRFRPTPPEPMRSFSLVLEGGRCLHLQLPPSGNGRSRDEWVDALQDLAATGKSRVAREERAGSTKPSKGLAAGSQQAPVTTAAAPAATAVTAAAGVAGAGAGGGATATLQVAAAVSGSATPQQHNAHQTPAATTWGPAAATAMVAAPTPTRIGNDGGGGYHAADGRVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSF3</accession>
    <name>A0ABQ5RSF3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001999</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038911">
        <property type="entry name" value="SCLT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35970">
        <property type="entry name" value="SODIUM CHANNEL AND CLATHRIN LINKER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35970:SF1">
        <property type="entry name" value="SODIUM CHANNEL AND CLATHRIN LINKER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1348"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="629"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="818"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="343"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="408"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="501"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1367" mass="145731" checksum="17E2920C47FF14E8" modified="2025-10-08" version="1">MASQSGKRHPVTLLAPSGREQPVILVVAREGVRLTTVDGKIINTFAYETIRKWLPSHLRSKNPGPDDCLDIQIETDKGPRDLRMRCASVTAVKQLMKDLRDTVETIMRDMNDDDWVTTESPSPQRPLDEAARQQDVGTQGQQAQAIRELPSIAATVPAATPQGSRGPTTAISQRSESAMRRSNAETQTPDEVAQQQRHQPQTSSAGGAPHHYGVAPSQPLILPSHGGGVAQLFLAHPTGVAVPVNSRGIPTPALLEGLQQATLPITPLVPSSGAFAHEFMEQYGIGGSASIPDELDVVERSSSAATGFVNAMFQQARAGIPVYGVYSQPSPSLPPHSPPLSPGIPSAGASSGGYYPQSLEVHTQPPQPAPMSVPTPQMYYSAPPVQPGHAHAVQRPQQYYQPQPPHQQQPAPQQQQQQHTLPPHYPPLPRAGSQRSMRTPSGYPSGHPPRRSDPTSTSPVRRRVEFSGVAPGHMSDSELSDSVGVSHYAHYAGGRPGAEAGGTSSSTGDSDQGSGSSDDPRDTRSGDAGDGMDLQHQVRFLEDLVSRLSAEVSRRGISPERATAAAMALSGAVDTTMAMSPATAATPVAVQGVSAVLPPPPIDYEGLLSADALGAAAVLRDQKLLAPLLAAYDSRIAQLEADLRSRTEQVQALKGQVDDVVEENDRLRTDLAAASDAREAALAQANSATAAAAAANNAAVAAAAAGTGNNEELELLRRENELLLTQQSEMDAEIQRLHRQLQERAQEALRGSQEQSALMAQLQQASAKVQEADGRAAKLAEGLRTTEAKAAALATQVSRSKAALDEANAATATTKVALEELRHAHTQLQAEAAAAQQRYKVASDGAASLQLELSTARERADTLEVQVSTLNRECETLRGALGSVEGKLTELQRRDADVWTRVKEAMMAAEEARLARDAAQARCMDLERQVELANTRLLTVRQATRDTVVDEFNTQINAAESAAAQVREELVAAQAAVSEWRARSERLERDQASLTAEMASLREDTAAALASKSRLGLASLGAVEKVAAVERERDEAVAKLESAQRKAERVARDAQMERQSMEHTLRGLKSTVAELETSLAAARAEVANGRRQLEAAGRELTAARAARQQTETELRQQMAALRADREADMRAFTSKLEAQATAANAAAAEAERLLSNKQEVLARWREEAQTLASRMEAALAEHKRELAAKGHEAAELRSRVEALSAENVELAAALSELEAKVEDLHAQLADADARADAAAAQLMSASAREAELQHELRQIQMVLDRTRFEKNRMQRAHDPLTAKYDALKKDIQTISVSVPTATTTTAATAATAAGTLPLNLSHAAATGISTIGTTGWAGSPDAVVHASRAPRGGSNHLLHTHPLGLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSH6</accession>
    <name>A0ABQ5RSH6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14312:SF1">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14312">
        <property type="entry name" value="CREB/ATF BZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="341"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="236"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="356"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1132" mass="121259" checksum="CC45C197F06312F5" modified="2025-10-08" version="1" fragment="single">MQNTEYSPFILLSEASPLTKWQSVCSYAIINQFQAVAAAMALHPVATASAVQLAASSSSDLGHLVLKALQWSSSELPLHRNLHPSELPRRDDVDLTEYLWEATEDGADKPWKATPPQHLRTGRPSPRPPHRISPRLPSSPPVNRQSLAAPAAPVAWESQGRDSIGTNEEHNGIIPERAAAGEEAAAATIIAVPSLSPPFAEATIAATAGAQGGGGGGALAVADTELIAEYEETEQQRQGQEQKQQQQQQQQQERQHHPPGQPAAARCCTQYADMGSSWSSAMGIAHMDSVFLDSQGGPTQGHEGQKSRQHQLMQDGGSQHVTGIHMVDTELQQTEIIRLAASQTQQQQQHRDEGQPEAAESPEPVAELSLPPATPAPAPAPAPAPPARPAPLPAPTPALFDVLRMLPADLVWRNLLSRIGVREASALRLLSREWDEQVRNEWGITLRLVLGADCVRELRRRVEGGYSQSLAARYAAVSRLQLVHRRIERTIFPNTRTVGSSGLLEVPPVVVDDAPPLAWVMQSLVGELGSRLRRLHISSDSAAVSELDLELVSMLFPNLDELVICFDCHEQPAPSLWALNALPRLRRLAVNLRNEKFNYDPVPELMIRSLGSLTSLRCLSLELTASSLPLPGRVLEHLTSLRLAVVFRGYSAGLESEQLRGLVSELGYLTALRALAVPDGYRGIDGDLLRAACMVLPSLTALQMPRLSVTRDELAALARLPMLQHVTLGSLDIAPDADYEDLLMCDDTLNDKPWVPSMDTWRSFHHGAEGHASETSTSCEMASASSNWSDSRVDACFVNRVGRNAIGGYSNGAEGNGDGSGCGGNGGGRIGSRSGVLRSMTSWRQFMLCEEQRMEALVAAGPLPPGLRELRVEQLRWPRGSRADNMTARSINTLLSFCADVAKLPYAAIHLSLEVLPGAATATFLKPPSEGRGAGSTTAAAAELAAAVAAGGGRRPLCCSSVRDVVLMSCSALGQVPGLKTLRWWGLLPVLAHLDEAGISQALGNLQQVLELDIPGGLRGPHPSTSPLFLLHLARGLEAMPRLRRLRLTSTVPHNDALAQGLPGFLVALRGTLRVEVAFVSYEDAAALRNLMARSAAAALVGAARTVDGSGREIGATDTFVCDGSGGATVMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSZ2</accession>
    <name>A0ABQ5RSZ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Radial spoke protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_002801</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flagellar radial spoke RSP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009290">
        <property type="entry name" value="Radial_spoke_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21648">
        <property type="entry name" value="FLAGELLAR RADIAL SPOKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21648:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06098">
        <property type="entry name" value="Radial_spoke_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="244"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="429"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="468"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI60708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="60751" checksum="9F38B002A44CDC74" modified="2025-10-08" version="1">MSRTEARVLSARGSLASRDGGLEDNLQWYLKAGRKQKSANMVNAKAQQQLYTHAAEPKAVQQRKAKYREDEGVPLPTANIMFDRRVVRGNTYAARILPADASQTQTKPSPPATKTKTTRTVPPRTPEAVDGRRHIDIQTDVYLEELTDTVPEADTSTQTDAFLDRPPTPLFVPQKTGTDAVTQIENGDLFDFDFEVEPILEVLVGKVLEQGLMEVLEEEELAAMRAHQEQFEQIRNAELVATQRMEAAERRKLEEKERRMAQERERVERERVVRQKVAASAFARGYLTGIVNTVFDRLVSSGYIYDPVMREVETGFMPWLKEQAIGYLSRGIVARQVVNRLLEDAAAALAANREAVVEKAKATAAAVDAWAERQAALEAQLQGAELEAVRRRPTFVLRQLKPAVATPEAIEAAAAELRAQAEEAANAKFEADRAEAADKARTEAEEKAETQRVKLEELTAQAAAEAEERGEDPPEDPPTLDEPLVDVEAVVAAAVEAVVKPPVKEVEDIDILSYMLDQGIVSKDAIIQSLAVHALGDSAYINHPAFSETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTF3</accession>
    <name>A0ABQ5RTF3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2061 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018638">
        <property type="entry name" value="DUF2061_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09834">
        <property type="entry name" value="DUF2061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2061" evidence="1">
        <location>
            <begin position="104"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="161" mass="18024" checksum="0281C968F3FE0FA2" modified="2025-10-08" version="1">MASFLLKQCHGFGCKARYQHQRRSLRLLCALSLERSGDPVSRKNKPSAPAAAALSVVERDMVVREEVQNAYEAGYQAGLAAAHQAQSSEADKARPQHDSLLRSIVKAFSWRFFSTATTIVLTISFFNATLDEAIEMGAAEFVSKYILYLIHERLWILVTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTR8</accession>
    <name>A0ABQ5RTR8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein assembly factor with WDR repeat domains 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002902</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="90"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="132"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="175"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="217"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="259"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="301"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="343"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="385"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="49142" checksum="38DD870787B3D06A" modified="2025-10-08" version="1">MAAKLKKFLLRYYPPGIILQYERDGAMKQKPIDLLDLTPDVDVEVLLSQIIRQEPLISENRRPALRQLINRLIDKMLEQQHHSFTLFKVLRAHILPLTNCAFNKSGDRFITGSYDRTCKVWNTFTGEEIFTLEGHKNVVYAIAFNNPYGDKIVTGSFDKTCKLWDAYTGQLYYTLKGHQTEIVCLSFNPQSTIIATGSMDNTAKLWDVETGQERATLQGHRAEIVSLGFNTGGDLIVTGSFDHDSRLWDVRTGQCVHVLSGHRGEVSSTQFNYAGTLVVSGSIDCTSRLWDVRSGRCLSVKQGHTDEVLDVAFDSAGTKFVSASADGTARLYHTLTGVCNHTLVGHEGEISKVAFNPQGTRLITASSDKTCRLWDCDTGECLQILEGHTDEIFSCAFNYEGDFIITGSKDNTCRIWKALTASSQPGVPGGPGARPQTAFSYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU03</accession>
    <name>A0ABQ5RU03_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="179"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="345" mass="36042" checksum="CA1CD89EE29189BB" modified="2025-10-08" version="1">MQPIVEAVNLIEDGLRRGSNGCLRSELINRCFLASLYEAQLAARSSPFAAGSPAKVGSPISEQELCSDILPKAMCNLGRATALAAQLREYEVLTFCAIGLAELQISCRDLTAASATFDGCVASCAAIADVPLRASLLQRLLPKSMEVCNMQGRTADSYVRLRLLNTVLALNGRCVTEECSICGKALSGDRACLALGCGHSFHRDCCDCWLGRKMFICGTFRAECPVCTQVDWSLKPTPAALLALEEFKAACASGNASGSSGGPSGYGLHRDDDVHRDAGSSSSSSSSGSSSSSGSGSNSGSTVKVRCTKNGCSYIKSESSSCSGSSDGRDETEGGPDDCAVSRMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU29</accession>
    <name>A0ABQ5RU29_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002908</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="4">
        <location>
            <begin position="83"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="119"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="406" mass="43642" checksum="09D61D7D7DB49A27" modified="2025-10-08" version="1">MAFCVFPKFTRYDVKAWTAQLCSKRHQHPRSQCFIQLRRKSDLCVEASNPGGPRGPCVGVAWLQRNLEIVRIYDARGCISETFDTTDSSTCSANYDAYLEGHIPGAVFVNWLSDGAAWQNRDDTGPSGGGDGGGSSNDGRGGSASGEHGFRTRTLAVVVDPDVYVPCFEARGLSTDRPVVVYDVGTGMIAARVWWQLLLYGHPRPYVLAGGWPSWLAEGGRSELYEPCPLKLSSVYDEEPQLHHRASPKEFRLAATRAEGEGEFIAHTVAVLLVPESFSSVLESRGITSSRCPDGLPLARCLSFRSLIRACLANKTDGGSSYSSCTGINSDRDPGGVATWTSVVLGVERDALAHFLGEALGGIPVGPCSHVRLLLASPHDAALSACAVGALLYAAGHEHWAVCEAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUF3</accession>
    <name>A0ABQ5RUF3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003324</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="883"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="349"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="481"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="911"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1232" mass="130366" checksum="74D804210F963B45" modified="2025-10-08" version="1">MALVCAMLLCSCFYRKDLRLRQSMPAIDVPNNVGDDVCIDQAFEKLKSDLLCLNGSWFNRVEGLLRAFNNHGLVAELYGLASSNNFIALLSNTRSGADKKREILLQRMSSLKTPTVHLSPTSNCGGKQHVVAAALPLVHGGDLIGALWIGYASLQLPKVPPWRHATAPGSSSSNNTSLLHNQEQNLHPVTENGTMLLAAALLSSDESLRQLSFAASMCLASSGSDMLMWLTETVSRLQVAANMQALTAELCAAVASHVSNHFMLEVSINLALIPPRTSGSRLMTKTTTAADGDADTDANTDANAGADAPLAAATALLFRVQNNFDSGHVPATDTNCDVAAAFPPVGASACRPKPSSSNPHGSHFGKSPPNSPTVLGGTNAAPQIRASPGNNVMASSPYSTLRSSMPVGGTALSVASAACSNLRTSGGCGPALTTGSVVVSLSAAEAAAFTAAAPTAAALHADVLPLQHTLLQAVLDELQKQRQQGQQKEQEQSAGGDDVLPPGSSNGSTCSPISVIENTCLYIQNVHNPWRDVWLFMERGLECIATPTGVSSFGSSLTTTTASGSSSRSPMLQSLALLTMPLDEGSAALGLFLCFPKRLPGALLEAVQASCQELLSKGLSGQVRAKLEGTLCSEYDMLKVAAPGSYAVLRGVNTCEDRTLGTVPNYTSYQTKQIGFLDTLFRRKTVPSMTSQQSGSMLTTAFKTYALLGELDSGPSLTAVLSGVEDSDGNDKPYQSPAASNASAWRRSLAIRRTPSESAARSIVAPSLAPTSATFANTTHDFAGFPLASCNGDVPDTWGSCGASRRAAGALATNGEGGGAVAGGGNQSNLAYGPFASIVTIASVDTQAHQRMDLLTYSIQATVLASAASNSANCTFADDLEQLELTEVLGRGGGGVVLKGMMSGTLPVAVKLMIAPGEAVEELGLSPEAVRRGMLRNAMELAVQSSLSHPNIVQLYATFRNAVLRARQLDTEDSRLMYTNPRSLYLQDASMPPRPQQQHDRNVVHSLDETRVTCILAEYCDAGSLEKALVSQAFPRLLRDRMAGMQPAPATFKYDMKGVFMTLLDVALALRHLHSLHFVHRDVKPANLLLKSNPRDPRGFTVKLADFGFVLRLTETDEAGGRYANVDEPCGTVTHMAPECLSRKSKIFAAADIFSFGILMWELLAGGSRPFPMIHPDKISGAVYRGARPTFAQHVPQSYQSLAMLCWRTEPARRPSAKQLVTAITSRLVALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUS9</accession>
    <name>A0ABQ5RUS9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003217</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="26"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="198"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="338"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="468" mass="49255" checksum="CBB91BD15B94AB8D" modified="2025-10-08" version="1">MGQVDPCRKHGLQHRTFGPVKLLWSSGVAVIASAALLFSCTSLAVKLLGTEVPTFEVVLVPGLICFIATSAAVKSHGMSVRSKSWRIAGLTILRGLLGAASITCFYLAIGLLPLQDAVTLFFCNPVLAALLELIVTGESQGWACATATACTVAGVVLVAQPPCIFIHHHGGSAEPHGQGQSQKYSQAAFLGSHTSVAGVALAALAALSNASAFVVIRLMKRSQSTLVLTWWYHGVVVLVSSIPLSLNYPAPPVLPSLRGCVLLLVVGITQFLGQLLLNRGFHLESATRGSAINVLQVLFSLIWDAAVLGDKPSIVSIAGSSLVVAGVVFMALTGSPPQLGERHGHPRHHHQHADSDMDEERPEDPNVAKPLIGTRPLPDGVGYSSLAPPQLALADVDAEAGVTGAWEVRQNLDVDGILDRSRSDSWANGSGDGEEGAITRAERHLQRAPAIYTAHTTYTCTLASESQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUX1</accession>
    <name>A0ABQ5RUX1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="712"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="776"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="569"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="647"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="720"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="737"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="791"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="814"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="837"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="847" mass="84576" checksum="78CC49918161C558" modified="2025-10-08" version="1">MAAVGSAEDSPRWPFPRSSSARSGHGSGAAAHPFQQHQVQMQQLPDSEKPPLVGQFSSSSESRPQVAWPEQGADSTSLLLHLLQPRRAHVVPTPPDWGGSGVSSPNSGGSLTSRVYSDSRCGSGGGAAATTDAGSGSYSGWRVRPRALLVKADEPDELAGQDSASASILDSSPMSPAGSGVSERRRRTISYSAHSPHSRQDSIWTDSPRLPQPSQLQNSRTASPAAASPTAAAAAAASVVTASAATATAAAGTYGPTPTLVLPRHPLSGSGTLRRREFTPPGSPRKEFPAADGGPTGQGPGLGRLAGLRASESALGHPSRLGATRGSFKAGTIKAEPRLGRTGSGHVGGSGLATVVAADIPGESSCGGNTNTSPALSGPFRARSVACSLMGVLGDSGGDGGIGEGGSGASMESDSAAPVMVMTARRSKRDLAVESSPLQPPGSRDCSGGGGGDGGGGDGSGGAPKALTAMSPLAGPLAGPAAAAGCCCGAEDTKYASAACSGVGNGGDGSVGVGTWSLRGGSREEVSAFDAASPQGQPQGKTTKAAAGAEEEEEEEEEEEMQSRTVLNSAPPTAQSQPQAPAGGRVRSSFTAAFQQREKSEGVGAWRLGSGEYAEGDQDGLESRQQQISRAAALRHPPTPRAAIGMLPSPLQSPSSRPPVAASAPVCTASPASWNASSTTAAAAAAASAAADAGADAGSPAAAAKRLMLSTRSRRSLEISPPSAAGAPPLLTLTQSEPPPPPPPPPPLPVPPPPPLLGLPSFLGSPVVLEPYAAQLQPAGPGTPFPLLLRSSSNNSSSSNSNSMEAGSAEQEAAVDAVAPPTAVPRLAERTGAGRTSEYEKRYLKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV76</accession>
    <name>A0ABQ5RV76_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003918</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="465" mass="51683" checksum="CEDC51025A336228" modified="2025-10-08" version="1">MSSAGAGSSSPVGSLLDTLPDEILIQIFGHLAIVGSVWEEDDPLELTCLPYRQSNMELMPSTGLRGYPFLAQVCRKWRRLLATPMTQQLVWQRLCIDLGHELVTSIHAPLRWSNQRPSNEEYQMALRRTSISASKVLRFLGGVAPHVRALALANTEGFDGEDGQYVSLVDKHNFGPSHLGFALALLQNTLTKLILYRCYDLVSEDSGFWTLVTQLPALRVLEVEGLRVLLPDVQVAALGEVRKLEALTLTGDEFRIGSTIPCGLEGIPAAWSQLTALTRLELRVHHLLYELPVWLTALGALRHLDVSGCADMEVGSITQLTTLHVLVLQSMHMGHALPSPADVASAALSNQELARHALPDLQPLAPCLRSLSLSNNRFTQLPDWLPRLTRLEHLDVSYNRDLHVRAPLTGLASLPYIRLLDFRAVHVSKDKDFWCEAKCTTMQHLSKLAKALKRRCPQPRLLLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVG4</accession>
    <name>A0ABQ5RVG4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_003318</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07098">
        <property type="entry name" value="ALDH_F15-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="94"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="628" mass="67801" checksum="7EE2AAF33941CA82" modified="2025-10-08" version="1" fragment="single">DLQTLRSHSMEAMQEAMHALRALVNQGIALTGFTETQLLQLAVAAVAALGLVYLFKDVISTLCIPTIKVSPTDNEANDIIHAVKYNPVQPVDKDVVPCYDPSTMQALGFKPAMSAAEVRTRIARAREASKVWRKSSFSQRRQLLRILLKFVVDNMETICRVSARDSGKPILDAIVGEVVVTCEKLHWLCKEGERYLRPEHRSSGIMSFYKAARVEYVPVGVVGAIVPWNYPFHNVLNPLTAALFAGNALVIKVSEHASWSTGYYGRMIDAALEAAGAPADLVQIVTGYGEAGNALVTGRVDKVIFVGSTQVGKLVMRAAAETLTPVVLELGGKDAFIVTEDADLNAVVQISLKAAYLNCGQNCAGGERFFVHEKVYDKFLDKVIPLIRNLRQGNPLGPEPVDCGAMCMPGLAEKVHGLVEDAVARGAQLLAGGQLRSVSGSGGQYYPPTLLADVRPGMKIWEEEVFGPVMSVVRWSTDDEVVELANDCAFGLGSSVFAGSQRRARDIASRLEAGMSAINDFGTTYMCQSLPFGGVKHSGFDRFAGVEGLRGLCVPKAVCEDRFPMLMKSAIPPPWEFPVKSNAVAFGRSLVTMFYGPGLLYKAHGLAALLHCLMFPGSYGKGGKPKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWI0</accession>
    <name>A0ABQ5RWI0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004047</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="92"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="161"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="214" mass="22507" checksum="A63BA5EEF8C9FF9A" modified="2025-10-08" version="1" fragment="single">ESDNMENGHQHNDQDGDVPTLSQDEYAWLQAQLAAAGLGGGDDSGDANDDDWEDPTGGPLRELLIATEEGRVERVKELLQGFPGDVNTPGPDGDTALHLACLFGHTGCVEALLDGGADANIVNPEDGSTALHDAAAGGYVEICEMLLAKANNNILAKADDDGDTPLHNAARGNHADIVKLFLARGADPYVENGYGIKPVDEAEEQLVIDLLQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWS1</accession>
    <name>A0ABQ5RWS1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">40S ribosomal protein S3a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004599</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03122">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018281">
        <property type="entry name" value="Ribosomal_eS1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027500">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01191">
        <property type="entry name" value="RIBOSOMAL_S3AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047363416" description="40S ribosomal protein S3a" evidence="6">
        <location>
            <begin position="22"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="272" mass="30714" checksum="715A29BB45DE90A9" modified="2025-10-08" version="1" fragment="single">LSQRFGIIACLLTTAAPMAVGKNKRISKGKKGGKKKASDPFAKKDWYDIKAPTVFSVRNVGKTLVTRTQGTKIASEALKGRVFEVSLADLQKNEDDAFRKMRLRVEDVQGRNCLTNFWGMDFTTDKLRSLVRKWQTLIEAHVDVKTTDGYTLRVFCISFTKKRQGQIKRTCYAQSAQIRQIRKKMTDIITREATSCDLKDLVAKFIPEAIGKDIEKSCQGIYPLQNTFIRKVKVLKAPKFDITKLMEVHGDYSEEVGTKLERPAAPAEEVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXX7</accession>
    <name>A0ABQ5RXX7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="499"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="625"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="970"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="970"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1003"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1003" mass="98065" checksum="FC310BF3216C0434" modified="2025-10-08" version="1" fragment="single">MVAANQLRATSHPMCVHVSSAVRDRLQPTADGEFTFVAATSRTVGGGPSGLGPARTYFAKLGHWRAALALHGAAAVNTGMGAGIPRVQGSAVQGARQVRRSTHSGRALMAAAAAAGAAAAAGVGTGADEPPLGHQHHRQQTKAQSTSSVEIGRNMSTLQQQHQHQQQQQQQQQLQHQQQHRRESGQAGPGPAFSSEATPANAAGPGAASIPSAITAGAAAAAIDEAAATAAAAATIRFSNVDGSDGAPQSSGFNGPRDASMPTHPSTTAAAAEYGAVAAAIATKNSHGSPRRSPPARVHSNLSNATSLFAPSTVNSGISSSMHHSYWQAHGSSGVFGTGTGSTAATGGSMSSGSSLYPVTASDGHLLHRDANNTSNTCRPSSTGPSKPAIPQPLLLLLQQQQGPSAALPTVDEDLGCSGPGGGSGSLGGFTAALYNLSNGMLSNVQLHSSVIPTACSTCQASSALEQPFSGMLSGSGVPAGEGAAAAAAAAQPPDKLGRVQALHRSESSTMHPSGSGGAASGTGTSGATGTDESMALRRMSGMGADPSPYMVYTNISDVAGGGSTVVLGKRFSGFGSNGVAVGGGGGGGAGRGGGDISGTPSEPTQQGITHSELMFLLATSSAGLPGPSAGGGTGGKDLTSDSYDDVQSTGHGVPSSGNNVIFAAPASLGSPPLPTVAAAAADGTASAGNGGMPGVAAGLVTASAVISDISVGGPLGGDAFNGDVDLDGELSVSVSAMAAASKRLESGTQTRIMVTTASCAGLTEADNDGGSEVGLSNADVSLSSVPSTQAPAPALVSAVEAMFTAAFGSTVDSRGRAPSVPRSVPRAHPAAAAAAAAAAAFGGSGGRASNSSAAGISISSPSQLLSHCQGSQRGLFDSSFKATSSMGGGLLANTAFPNATMNASELAMLLQVAQCSVGGNSGSSISFPHLQELVSATLRDVGAGRAPGSSVSAGSFNLHNSHLQLLNASNENQQNVTSSQECSDTDTPRSTSRDGGGGGGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY15</accession>
    <name>A0ABQ5RY15_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Pre-mRNA-processing factor 19</fullName>
            <ecNumber evidence="15">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">VaNZ11_004953</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Ubiquitin-protein ligase which is mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 21">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the WD repeat PRP19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="15"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16656">
        <property type="entry name" value="RING-Ubox_PRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038959">
        <property type="entry name" value="Prp19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013915">
        <property type="entry name" value="Prp19_cc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055340">
        <property type="entry name" value="RING-Ubox_PRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43995">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43995:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08606">
        <property type="entry name" value="Prp19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24814">
        <property type="entry name" value="WD40_Prp19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 15" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11 15" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4 14" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="U-box" evidence="17">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="257"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="302"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="357"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="389"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="108"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GLI62339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="508" mass="54880" checksum="2132A6F865E88408" modified="2025-10-08" version="1">MFCSISGTVPEQAIVSVKSGHLFEKSLIEKYVRETGKCPVTGEALSSEDLLPVKVNKTVKPRTAPATSIPGLLSLFHDEWDATMLELHNTRTQLHQTRQELSHALYQHDAATRVIARLLKERDEARAALADMKQQYAADLAAAQAEAARAAVEAKPELEGGRKAKGGIPEDIIADMVEINATLSKGRKKRPLPDTLATPEDLASLTLSGSNPLHKTTAGGVAAIDVNPQQQGMVVTAGLDATLQLFDYLQARLLGSLEGHTKRCTGVAFLNSELVVSSSADKTARVWRNSGDATVWQCASVLRDHTAEVVGVTAHPSKRYFVTGSTDASWAFYDVETTTCLRQIGVDEGPGEPYTYVQFHPDGLILGTGTEAKAIRIWEVRQQKAVAACEGHAGPIRCLAFSENGYHLASASDDCVKLWDLRKLANFKTIEPFQDGPCASVAFDHSGQYLAVGGPVVKIFGQKQQWSELKTLTEIPKRAAALRWGPDARSLLVGAADHNLRVFGVPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYV4</accession>
    <name>A0ABQ5RYV4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Histidyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_005555</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707:SF1">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="7">
        <location>
            <begin position="79"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="492" mass="54528" checksum="6BAA511E50EB50E9" modified="2025-10-08" version="1">MLRNRLNRGGGRTQRPFSSPATSQGPERIRSSGWCTKAAASDAPAVPMKAVAESRTVVGKITEPTGRTDKGIIDTQPPRGTRDFFPEDMRLRNWLFGEFAAVSTAFGFEQFDAPVLESEELFVRKAGEEITDQLYNFEDKGQRRVALRPELTPSLARLVLQRGRTLPLPAKWFAIGQCWRYERMTRGRRREHYQWNMDIVGVAGVEAEAELLAAIVTFFERVGLSKDDVGVKVSSRKVLQSVLTQYGVPPESFARVCVIVDKLDKLPRDKVEEELRVLGVPPATINGVLSAVSIQSLEELSQLLGPDSEAVADLTRLFQLAEGYGYKDWLVFDPSVVRGLAYYTGIVFEGFDREGKLRAICGGGRYDRLLGAFGGDNAPMAGFGFGDAVIVELLKEKGKLPAPPHRVDDVVVPLDTALRPAASGIASRLRRAGRSVDLVLENKKMKWAFKHAEKLNASRLVLVGTSEWERGKVVVKDLGKFHQEEVTVEDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZF2</accession>
    <name>A0ABQ5RZF2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cwf15/Cwc15 cell cycle control protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005531</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006973">
        <property type="entry name" value="Cwf_Cwc_15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718:SF2">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04889">
        <property type="entry name" value="Cwf_Cwc_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="64"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25912" checksum="6053E95B888C2442" modified="2025-10-08" version="1">MTTAHRPTWAPAVGGEEQGGMRIFKPSVAVSSKNLPAHTKLKFRQEGQSGLDEVRAKDLRAELDEKERRHFASKSGAVSNFEEERQRDLKLLEAAPPDGQTKQLVPKAIDADDEDPESSESSSDDEDDDDEEALLLAELERIKKERAEEAAKKAREEAEAATKAKEEELRTGNPLLAIGGEVSFNIKRRWDDDVVFKNQARGEPKAQKRFVNDTIRNDFHKRFLQRYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S008</accession>
    <name>A0ABQ5S008_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PLOD1-3-like GT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_005417</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22997">
        <property type="entry name" value="GT_LH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057589">
        <property type="entry name" value="GT_PLOD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25342">
        <property type="entry name" value="GT_PLOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046573492" description="PLOD1-3-like GT domain-containing protein" evidence="2">
        <location>
            <begin position="37"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="PLOD1-3-like GT" evidence="3">
        <location>
            <begin position="428"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="667"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="676" mass="74018" checksum="FC602874200E077F" modified="2025-10-08" version="1">MRQANLERLYRAHTSSPVLLLLLLLLLLLLPLLVWSRNSEDETLQKLSTSSQNRTAVWGEQLSGAGVSAASTADDFDVTSSDDGIVSDDGGPRRRRRRQMQEQDGRPPTLSHGLMHFGQARVAMVHGEISLGEQLWSCHQVANVAHRSGACAAAEQQIDTKCAFLELGGPGLCAFDLAKRMPSIPVNLYPSSRRLAELSLPATGSLESNFQLEGDLDQYNKGHPVVLQINMLRGRSSLSYIGMKSVAVNLLSRGAVGAAVMTLQAVSSTHLQLQFLELVGIAVSSGFSAFWGGFDGQDITSDLGRVLRKQQYGHRSINIFLLPRDVAAKEAPSPPPNRPPPPKLHVLVPLRPQREDERHGLTMLLCRFLLDTAAASNLSSVHVIGLTATGTHLYHQNSLIQKLMVLQYVLEGPDPSSGMRPWVDISPSDVVMAIDATDTMSQLTSEEFLQRYLDAGAPVFHVSAEVNIWPPPVSNYTHLYDEAGVNAVPLPNKYLNSGCFIGRAMVLKKYVQDAVEFVKTNTKSDSSWLFRQHVDYVDDQGVMSFAYIMGNPYGISLDHEAAFFYSLYCATDHLVLSCGAIMYDLTSTRIGLLHGNGHEGKSRLEDLRGGILGDFKPDPEFRFLVDGVVTRYGDTCVNVPPLVFGRPTVQCPPGGAQPEQTYTTRSVEPKDCPARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S054</accession>
    <name>A0ABQ5S054_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histidine kinase</fullName>
            <ecNumber evidence="4">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_005426</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal/bacterial/fungal opsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17574">
        <property type="entry name" value="REC_OmpR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001425">
        <property type="entry name" value="Arc/bac/fun_rhodopsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF71">
        <property type="entry name" value="HISTIDINE KINASE CONTAINING CHEY-HOMOLOGOUS RECEIVER DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01036">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01021">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="14">
        <location>
            <begin position="354"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="15">
        <location>
            <begin position="812"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="15">
        <location>
            <begin position="1036"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="641"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="706"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="754"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1550"/>
            <end position="1618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1853"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1949"/>
            <end position="1982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2001"/>
            <end position="2132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="650"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="706"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="754"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1598"/>
            <end position="1618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1862"/>
            <end position="1876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1972"/>
            <end position="1982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="2012"/>
            <end position="2035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="2036"/>
            <end position="2047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="2048"/>
            <end position="2092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="2093"/>
            <end position="2102"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="11">
        <location>
            <position position="863"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="11">
        <location>
            <position position="1090"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI62848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2132" mass="219562" checksum="F4BEEFA43D1C4356" modified="2025-10-08" version="1">MKPSKAGAKKPVASDVGTNADNPMLPWASRLWASVGLPWILTGLATYTFLTVLGRQFLHNPKGLEVLREQVPLSVYDRSTSIFLTALAIDLLNIIFERQTIKVDCVLLPAFIKGMASTTNLLVRFFVPVMVLTDAGRPVVVQRYICWMHTTPSILMLLKMISTTITTREALLAILADEVMVVTGVVALVTTGWQRVLWSVITHVAMLPVVPYMHKAFTECMATDRRMAAPLWAAVNRPRARDVVARAMEQVRTRPLQLTMLLIYVLNLLLWCVFAVTWDLALLGWISVLTEEVLYVGCDFSAKVLFSSTLMLSSFKEFEARRENAMRVIENSSKAKLITELQALLEQKERFMSSVSHELRTPLNGIIGISEGMLSGCCGVLPEGVRRQIYIIRTSGARLLALINDVMDAAALRQNKLVLKQEQLVLRHVVDDVLDLTRSLVDSEVTLANLVPPRMLVMGDSGRIVQILNNLLGNAAKFTRRGQIRVTARQVEGGRRVAVTVSDTGIGIPRNKLATIFLPFEQVDMSISRKYGGFGLGLNIVQELVKAHGCTINVSSIEGKGSAFTFTLPLARAMGRESLEEARAAAQRGSSLAGCTQDGSAAGLSGTSGSGGLRSGIGGASGTAVTVTGTTSASATVSGIMPRMGEGAGDDSSTVISSSDASRRPVGRAGQGTGRNPTESELEQYGELMYNAAELSALASSRPYTQSVRDSASNSLEGTNSAAAQSPLIGTAAPGHEGAARVTKRGPRAMKGAAGAVTASATAPASATAEDNGSKGVANEGMGSGLLGLLPGEIKIVHGPRPFHYTMYKRFLLLSVDDDPVNQSVIKSLLGSTGYEVVAVSSGLEALRYVSTAPALPDLVLLDCMMPEMDGYEVLQRLRTMTPSVHVPIIMVSAQTEEEHVVCGLDLGADDYVTKPFKRNELLARIRAQLAYGEWEHDEGALETISSLMAGECHGNVGIHPHNGAVELDSGNGEVPGGSGAVGASTVFNPAAAAAAADAAAAAAAAATAAAVAGRSQTRMTTSLLEASGSQGEQRLIVCIDDDDVNQVVLQGMLTSQHYRYVRASTGSQGLSIVCGTQNGGIPPDLVLLDCSLPDMTGFDVCRCIRQMYNKQQVPIIMLSARHNESAVVEGLKCGANTYVTKPFRRNELLARIRLHLRSRDPAQGPPQSHGDSGAVDSEFCAGSAATSVLIDSAVGRDGSQNAHRHPGITAATAATGYGGPAAMTSALPAAGGSLAGGGAAVDSVEGSSGVRIWMEAAVLVVAVADYAGLCRMLLPAEMAELSARMVTAFDSAIAAYGAVKIEASSGVMSAAVLPAALPPLMGAGTDADAAAAAAAAAAAAAGLRLAALHRVGRALLEAAATLPVPGTSTCLQLQEALALGTIMGHSTAPAAGSTMLYFGPVLAELLDLARRAPPMTMVSPDGEAQALRASGAAADIRGPLFLEPEDGGGGSGDGGDGSTAIAAANGATTARRLWLVEAHPFAHQYLPPQLLFSSNLMRGSFFQRSMTFNELDAPEKGSGSTAITVSMHLAAAAAAPVPAAVQLQPSLQLPLPTASHPQPSPRGLSTPMATGLPAAVSGPGLPSAPAVSLPGPPLPLGPSGSSSSAATATAHAAGGSSPVFTHPITGIMGSGDPGHTSLSNSIAGFNPNMGLYGYGLGISRAMPRALSQTAIYNGPDSEALARMVIMQPFQVLPPPPPPPPPSLRLHPILQHGAFANQTHTGGGNGGGVNQANGAGTGGAAGGTMSAAAAGGGISGVSSSLSQNYTLGSPTGHGNARESASGGPCITFSLQAAAAAVAAAAAAASGGGGGGGSGHFNIPPEPLLMTYGTPQAGGLSPNVGLSGMSLLYGNNQRASSSASAGNNGGYPGGGGGGGGPPLDPEVTAAEPQIRALRNELATMRMQLEALQSGASRDQAAQMMMPKQGLMSPQLQLPPMPAFNIAAVGAASPSPGGNSKAWFASQQGAPHDGPVKASGGSSATATTIGTGGAGSTFLLQYPPPLFQPHNALPPSSPALTLPSVAPPNQQQHPQPPQSLEEQQRAHQEHQQHLQELQQQQLQQQPHVGASQEAQPPAAAAPAATVSAPAPSAATSTECANSSQQPSDTRGAEKAGKKSGSKWSRLFNKSGKGATSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0J9</accession>
    <name>A0ABQ5S0J9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RecA family profile 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005883</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030547">
        <property type="entry name" value="XRCC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46644">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46644:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="2">
        <location>
            <begin position="26"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="518" mass="54405" checksum="095D2C43DD3F2D73" modified="2025-10-08" version="1">MIDLPPDIAAILAFNETGAQFFKRVQQERVLTGFPIIDNNLSLRHGVVLEVVGPSGSGKTELLLSIALHVLLSPYADPKAWLAAPNHQPKEQSQPPQRSGPQHLHPAEGTPREGSVRHHQQGPQPSSTTVTASGGSVVIFDLDGKFDGIRLMQVLEPQVHSAWRDQQLQLRIAARNCSGGAAAAAATAATGHPISDAVVTATATATAIEVTDAEPQMLSPDQVVEAVLSRLHLVRCHSSLQLLTALAALPKRLEALQAEGSPCRLLLIDNVGAHYWRDRAAKDLPPGTRPTTSKGGGGNYPSPYHHEPPHHHHQQQQPHQPPHQPLALSLFEMHAAIAARLSYVSRRHRLPVVATKTAAVSATGLPEGGGGGGGGLEAAAGGVMEGEVQLQQREFMPITWQNAVTHRLLLYPRGAVVPGGATLPPSPRGKQPHLQPQPQPLTAILAEMLGADADPAAAAAASGAPASVAGTAAATGNRVGNNVFLKLSAAATAGGGRPRGRLRVTQLLVSSVAMVEQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S108</accession>
    <name>A0ABQ5S108_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_005800</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF268">
        <property type="entry name" value="FAMILY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045198298" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="794" mass="90576" checksum="BD3E4C432195BCCB" modified="2025-10-08" version="1">MHSIRFLCILWAWMVCTANSSIPGPLRQLLQSNPRNEKPIELPPRKFLQISGDFQTAHKHSQQHHDTSSSIYSAARRRCRVVRGTWCGEFIRQVEVPTRPVPRGDSDCPNNCSGWGNCNHDTGLCECPAGRGGQDCGSEVKRPCTKQLRDISIPNVTEPMSFIGPDKFDLNVTVPGWTASRCFGYCYDDLAACFCGDGKYRHIPAPPGSPPWTPPVQWGRPIADGCQPRNDSAGNPTFFAPAGPTYEDIYGPEGWCNHPQPKHRCGCLMEIAEPCDFSQPLETMCVNQCGGHGDCFFGFCRCHPGWYGSDCTRKRAGLALEPARHLGNDAWLEPLIVIPPASLPVPASPTRPRPLIYVYDVPPEYTSRMLQYRVAADSCVWRRYYEGNNTGFTGWTYSVETLIHELMLQSEHRTFDPEEADFFYVPVYVSCFFWPVMGWADHPWWYTPNGLRPMHGANMLEEVHQWLTTRFPWWARRGGRDHIWLMSHDEGACWMPKALYDTSIVLSHWGRMDVNHTSNSAYHQDNYSGVMDRAFYKWRGTDFQDRIKGHVCYDPNKDLVIPAFKPPGHYEWSPLMGARPMDRDILLYFRGDVGLNRKPHYSRGIRQTLFRLAAEDDWYGKYRIVIGSSHMIEGSYSEQLARSKFCLVAPGDGWSARAEDAILHGCVPLVIMDEVHAVFESILDWESFSIRIREDDAVLAAVPELLMSISPERLAKMQRNLARVWHRFAYTTSPILRKTVDYTVRLNTEMLPAGVEGPVPQDSPYQPVTSFPYNDDAFHTIIQWLYHRIPHTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S198</accession>
    <name>A0ABQ5S198_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="646"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="919"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="994"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="928"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1015" mass="103375" checksum="EE943DA22E2406B8" modified="2025-10-08" version="1">MLQGPGRNSPLRVPLLDPFEAASQQGFPANDGLDLRESFRRMATDFCQQLSLAGPLAVNLVTANAVAVSILYFVARGGTEQLAAAALGNNVAVMFGRLVLLGLCGALDTLAAQAWGAGKERRLPLLLQRTVLFLWLHCVLISVAMLLLPAGLHVLGQDPALSAMVWKYLLALLPSVWLEALSRPINRALVARGIVTPQMVISLVGLPLTIATTYTLVVPLRWEYLGAALAMSAAAGFEVVLLLLYVAGSGQWANVLGTPSRKALKGWGQLARLAYPAASMKCAESWAFTICTLAASLLPDACTATAAVGVSYNVYGVLFIAFVACSTAACMTVGNRLGAGDAGGAKLAAAGSLLIVPPAWGLAAVVLLVRPCQDAILALFATGQGPDAADDPTALNGVLRHMFVIVAGLVLLDGIQTVLSGVIQGCGRQHAGALVNLAAFYVVALPLALFLAFRLPLPDRLGGGLLGLGLGPEGLYLGMAAGPAIQTVCYSYILLSINWRRVSQAAVSAAALNSVSSFASLIRRLDSGGLMGPPLIRSAASSGHGMRSFSTRLPIIQEHLREGRDSSESFGAVAVPISISAIASGSASVPASSSAAASFNARMNNWLHANKAVLVGAFTGGGGEGEGEGGDGDLDGGELYVGASSDDEDAVNGDGNGVVTPVDPTLSTKRSLSRLRPVSGGDTATMSAAPEKRLLAAAETTAVGLLVPSLPGADDTPQAALLHARIMIHGSPPLTDPSHHCLLAAILEPNSPATTATSVLLPAGSVSSCAHSPCGYGHTHVPVGDVTLTATAWAGDKAVTDGGAPVEVVRPPAVAWWARLDWAWRPLLFRATAVPAKDSPELAAVPPKALSVAAAAGTGAATVSAPLQLPGVGVGTAEPLVAVMSNATGAFAGGLLSSAAPLPITGSMARHAAAAAAGGGSEEVHGLSRSTTDSVTRVSTPSSGCTSHLEPTISEAATAMVVARGDVFRSCESLAAAVPSSEDVYAFSASAGTDGSSWLDPPASLHQESLHTRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1S4</accession>
    <name>A0ABQ5S1S4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006689</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051292">
        <property type="entry name" value="Xyl/GlcA_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270:SF52">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE PROTEIN GNT13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047282826" evidence="7">
        <location>
            <begin position="23"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="453" mass="52715" checksum="7C96287134F9E1B9" modified="2025-10-08" version="1">MRGDRALRLHLLLLIWLAATDKNPHCSAARDDPKAIELMDAINDGYQLRLTNWNKAIMNDTHSNLKLERILWSSKPPRFQFTIFTQLTVNRLPTLFNMCTTYTGPMSAAIFLSLVQQPVPYRLQEDQSFNSEEPSLALERLSAPNMVLVDEAVQQVQDFHDKVESNPDICQLDVMLFYELYDSEQARLLYPVNYLRNYARLQVRTRLLAMIDVDMYVSRTLSEEMAQEGRVAHYENLCADRRATILPAFEPTRPGSKGRMMAMKLSKVSKSQLESMHGRNKEAIQFKLRVFPRGHTPTNYVRWFSEAQTYKVSYKRFYEPWFITCDEIMPWYDVNFRGYGMNKIVLIASLNYYNYTFWVHPDAWLVHSPHLDTDVRKLVAREASDVNKHNAKLPPNALYRKLTLLFGKAKRGMMRGTYDPRVDPRMMAVYDKVSWLRSPPKLVGTPTAESVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1U3</accession>
    <name>A0ABQ5S1U3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07245">
        <property type="entry name" value="VOC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050383">
        <property type="entry name" value="GlyoxalaseI/FosfomycinResist"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21366">
        <property type="entry name" value="GLYOXALASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21366:SF22">
        <property type="entry name" value="VOC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="78"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="201" mass="22424" checksum="E41B1733B54BA31A" modified="2025-10-08" version="1">MASMSILPRLMPGRYRSSRAQTVPVVCAKSFFGHFNNGPSVPYNKYGIRQVSSLLTASAGTTTVAQRQYVAGKVDFKGVHHVALLCQNLERSLEFYQGILGLEINPERPHNKLPYRGAWLWIGPEMIHLMELPNPDPLAGRPEHGGRDRHFCLGVAAVEPLVEKLEAAGVPYTKSMSGRPAVFFRDPDMNCLECVEMDSWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S210</accession>
    <name>A0ABQ5S210_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="464" mass="46393" checksum="78B198D982509647" modified="2025-10-08" version="1" fragment="single">GEGPGSLAAELLDSGSGLSGSRGAAKLGEIFAKARQHQQQQHQHHHHHHHQQQQQQQQQQQQQQQQQHQRHEEGEQPNSSSPAPGTTRSQLLSGSDGGETGKHPHGFHSKSHLLYGPTLSHHTATATATAAAAGPPAGSPLDRGEMQMYGIPVPAPHLGGGSGPSASAASAAAAAAAAGDVFSQIQGALPAGAVLDVVVPSKMASVVGVLYRMEQSATGGDGSGGGVAGSETWAAVWNGKVQRLRQLGAFASEDRAKASCQAALAMLQDNELVTTATLATGTAAPGAAAAAAPWLVTSANRHACHTAVPGVANPCSPQVQQSAGGGGGGGGGLGTERAAPPRRNGCGAGSPLPEYVAYDCAAGRPSGSVSHLGSASAGIDGTIPEATGGGGDCTAYKWYMPTGSGGMSLPKLGGIKLLAESNHPSNSQSPFVAIAMPQPQSSAAAALSLDSLGYRLQPVLLGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2B0</accession>
    <name>A0ABQ5S2B0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="264"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="639" mass="65222" checksum="3CA1F331E90DDB3B" modified="2025-10-08" version="1">MMNLGRIIDNALELAETLLATDFTADYLKPAKQPFIFAAAAPTATEVTQQPTADAPVRAATPSRSGAAVQAARAVCERLSCALAATEAARISTQIANLKLTNTPPQPRQPPSVQQQPPLLTSRPGQITSRELRFYAAEASPIRAPAVFHTGAASVSLTATASASAGNSGSMTSRGPRISPFATLTIPAAGPAAGIGGATTERLSPLRPLLTSRPSRLGAVPSLMLQGTSLPPATSHLQNYQQQPPAAGNPQEQQQLREHHQHYHVQPQNQPQLQEQLQKQFHDQPVETPSEQQQQQYPYPQPHPPLTSVASAGSNHNVVPAVVPRLSLPLPHHHHYHAAANLNPNLYASGPAEPTAATPSEPPTATITATTKAPATAVATAGGSSADGHPHADDMGMGRKSPTAPSPTAKALGSIFPSSEGQVAALQVVRPSPAVTAPTLSNGSSTSRRLGDPVPTPQYSRTAVAASTAATAPTTVAIALKLPLSPVKVAAATGVLPPGNRSVLATGLPPRGSSVTSGKGEKGISRVAKGVAVKPTGVVPSLRLPLEDGAVAAGKAAAQIGPPGLWRGRQSYEARRESGGRAGSGSEDGGNVVVEAGGAIAGAGAGGMGTGSALGGGEERRPTQRQAVVPSLRLGAMAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2C6</accession>
    <name>A0ABQ5S2C6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006314</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="133" mass="14965" checksum="B5FAC6E475B7BCE1" modified="2025-10-08" version="1">MKTFPGLIFESDSVAPGLLVDYTVDKKIVSFDIQGASKRLHCHFWDTSEMVDDKPSWTLTRLYDARGDQLVVYLTTSTPGLDDMDTKLLATDDPRISVLTDVHGSWQGVVVYEATKAIAQPTFENAPQAIWWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2D6</accession>
    <name>A0ABQ5S2D6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal RNA-processing protein 43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_007229</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF9">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="10">
        <location>
            <begin position="46"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="11">
        <location>
            <begin position="221"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI64065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="309" mass="32399" checksum="B174CB2EF57D0A3A" modified="2025-10-08" version="1">MAAPSTTATASDDIDAEAFKRLYPEQYYDRFLSEGVRPDGRPLGRARAVTIGAGAITSVDGSALVKVGRTTVLAGVRLEIIRPDEMSPSLGQLALSVELAPFSSQEYRPGRPPDHVSVITQHLTTALLSGGDAVELTSLCIAQGKAAWRASLDLYVLDADGCVLDAALLAVLAALRNTRIPPVRTTREGHYVAVGGGRAGDAGGEGIIESSPMAMQLGRCPLGLTCCLYRGHLLVDPTADEERLAECCVSVVLDQHGRLQGVYKLGGKVLADAGTLVKCTEAARLRYRELSGLMELSLGQAAKGTGGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2J8</accession>
    <name>A0ABQ5S2J8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HNH nuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052892">
        <property type="entry name" value="NA-targeting_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33877:SF2">
        <property type="entry name" value="OS07G0170200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33877">
        <property type="entry name" value="SLL1193 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH nuclease" evidence="2">
        <location>
            <begin position="351"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="475" mass="50324" checksum="F08A57983098A13C" modified="2025-10-08" version="1">MLKRCTSTGRVQRMQVAAITPAQTPSNMVQSFLASSATAIWASPRCCRLHNIRVAGASSHRRQHLCYALTSFSTPTSTSNGGDGDGCVVSRHNGGRYQVAQRRHLRFSSPHLAIMQLGFGSRMCRGRMNMNTSVGRVIVACMPSSAGGGSHQRRGGKRGGRRSPKPDSATPALTAGGGTAAGNGGNSGASSLTSGPAPSAAFSPVPAPSAIGGGGSGAGSGGKGVTTAATRGSASTGSSAAVAVATRSLDGSDSDYEAAAAGASNTVGGNIVPFARRQPRALVLDCAYRPINVLTWYKAFHFDYFGKGEVLEYYPPPAVCTTGLGDHPLPAVLRVPQYTADMQDLCSRVACTRRNIMVRDGFCCQYCGSRRDLTLDHVHPVSKGGKETWENLVTACMRCNQKKSDRTLRDLGWKLRRQPKEPTPFEIGIVAGISTSDILRPPPEWDAYIAPYRERLEMYRRAAVEAGLVNLIDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2X6</accession>
    <name>A0ABQ5S2X6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007464</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050997">
        <property type="entry name" value="MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10250:SF26">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10250">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="16126" checksum="F4CC0AC8F2F654EC" modified="2025-10-08" version="1">MNTLPKEFGLVAGVVVGSWIVHHGYMAVKVLQARKKYNVAYPALYATAEECPNPEHRKAFNCVQRGHQNSLENQPIFLALLVTAGLKHPITAAAAGVVYLVGRVLYMKGYSTGVPDKRLRGSLMYVGLFTLIGIACKSGVLSALAVLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3G2</accession>
    <name>A0ABQ5S3G2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1381" mass="145212" checksum="92B7A8E1087986BD" modified="2025-10-08" version="1" fragment="single">MAETVPTPGESEAARELGAQLLDRLQGQQSLPLTLRGHVQQASLLLGLRSESSDSHLGSGGPSSSSADVKGQPERELRVRRPSVAPQPVAQQPPGKRRKQVKLLPQGTDRDLTSTQPQRSLQRQRPRSETQRQQQQLPLQQQQQQQQQSLPPRQPRQQRQLPRLGAVATKSGTAASPSDPSPFPAIGTQESGLTRLRPDATEAAPSDSNSIPTNDSIVASAAPAACETHSIVVSQTQGSMATLSFSGSAAMLPGNNSTPAPSSPQPPLSSGSAGGDATGVAPTAAVAATLTFEEALQRAWSAWRDLDASDRGQAQAQKQAGRGRLAAAPVAWSSVEAALQGRPPSAMSIGDIAEVVHLLATLRLMPQYDWLCAAQDCLSREVAAAEAADSGDGETGRGNAVTAASGSMEMAAVDMLEDLRQVYAATEDLMQMCWEADERRKGFGELQGPPADPSTAAQDAGSLMPARSLMQDQQQRRELQEEKSQQQPLLEEKRLATSPPADDGGQRGLAAVATVSEPMCTNTAAATGISGNSEQGSYRTTAELVAIEPGGVTPVTQANSGSVASRAADTRNKGGEGAGAAAHVPSFDARALPSDPQAVFPSPQSSPPYGTGLPLEQEDSSSGILKGSFGDSHLADDGDDYAGDITDPDVSLEQLRALLGQWDRRAAAVVPKEASKDGVDALRVGNASYTAASAHAAVEELSLLLAEALRTEASATRTLGGAGATAADARPAAERFRALTAEDLSALVMAARGAQPVGLPRQLLATAAKLLVTLRGGATCEQVAPVLAACAATGCTPPSYVLEQLAMLAFPVQVQEQSDSHRTKTQAASKRQEPECAEVSNLSTEPSAAFVRAVHTCGAALDRLRFRLNNRQFGDWVATVARRPVALKPAQIAQLMAACTERGYTVRPSGAPEALVAAATQPITRLRQLSCSETVAVAIFAAKQGIQLGNNDGSGGSNSSSSTRCSGLAVLLLRHLAPQLGELGTSEMAALVPALYTTGALNGEGAVSTDWLAEHSAALLPHAEVLQVDQMLPLLQCYNQLGYRPPALLQLGLSLTLESHFTAAPLLPLLQALHQLLCGRMGFQPNEGLAAAVHDNLLPRLMLAATKAVSAAAPPLQQQQQELLQKNKQEGLSAGGLTMRQRLMVLEVLGAARVRPHPAQLAAMLEVQLAGGEGLMAELPGDALVAILWQCVSFRALPSWRFIGEWLQTFATAVSADMGARQVGPGALARVGWCLVQMGVQPEGGWQAAYHAALLPVLPKLTAEQLSLVAHSALLLRSRPSDEWMEALLRAALGRMRELDGASAARLLHFAAAGDVEMSKDWMQSFFLHTLCLVGCPTISESPSVQRPHHFGDAMNPNHMALMLRALGRLGLRPPEPWLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3M6</accession>
    <name>A0ABQ5S3M6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007366</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051801">
        <property type="entry name" value="GH28_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339">
        <property type="entry name" value="PECTIN LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339:SF9">
        <property type="entry name" value="PLASMIN AND FIBRONECTIN-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045984515" evidence="5">
        <location>
            <begin position="25"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="638" mass="68408" checksum="7107F3BDD982D4BC" modified="2025-10-08" version="1" fragment="single">MKLHRYSVHIKAILAITLTHFVSSSWDIISSSARRCKPEAFGAAADGNRLDTKAVLQAITACKEGGTVQFQDGKTYRLGEITVTGRGIQLEVPPSTTLLASAERKHYGARQDSWYLLHFHRCFECGLSGGGLIQGPAWDFVDNSSKPSGVLNWRDSSCRVRRQCRPRLVGVCRSSDVLIANLTLRDSAFWTLHIRDSDRVLVQYLTVKGDMDFPNNDGIDIDGSSHVTILQSHITTADDAVCLKGTSGGRRAVRHILVSNCTLQSRSAAVKLGSETRAEMSNITFSYLKVLDSNRGLAIQLRDHGNIRDVAFRNIEVATRRYPGRWWGGGEPIYVTALPRWLGSRVGRVVNVSFSNIVADVAGGVVVLAGSPESILREVTLENMRITIAPLADAYRVAAAAVGTPAPSTAAAAVPGIGVAKNRVDAGPCGVGLAPRRAAVQVRAAVTATAGALPQVRGGQPEGDAAATQVIQLWVKLDLRPGPYGERRWLAAAPILAQFTQSLTVRNVSFEFWTPVVAHDRGPTHGERSSGSLGAEAGARMDEDAVREPRAGAWSSQASNRSGALMASRLSDVPAGGRARGDASWYKELVQHTVGVQVAEQVEIRVVPAASLGWQAFRGDGADVGQWSGAQGGGFQWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4D1</accession>
    <name>A0ABQ5S4D1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103441">
        <property type="entry name" value="PetM subunit of the cytochrome b6f complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045911543" evidence="2">
        <location>
            <begin position="22"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="114" mass="11825" checksum="79AD67FED2337DB5" modified="2025-10-08" version="1" fragment="single">SFRQFHLFTLLFPAEMAMSLARTACSGVAAPRSATTRVSAARPAVRPTLRTVGQRAPAPSRGVATKAINELAMIAGEGGFIAGTALTMVGITLVGLAVGFVLLRVESLVEEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4H3</accession>
    <name>A0ABQ5S4H3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_007346</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="90"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="330"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="478"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="547"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="603"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="846"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="974"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1035"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1156"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="480"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="556"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="614"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="979"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1051"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1079"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1169"/>
            <end position="1186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI64161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1299" mass="138712" checksum="3D611DFD97352C03" modified="2025-10-08" version="1">MESLDTGVTICRTGQDLEETSKEEDGCYRRPGSPPQPAPLPHRPAAVRARTEDVVSDAASFRELMISDSLVHSLVTAGFERPSPVQKAAIPLGRIGTDLIVQAKSGTGKTVVFAVICLERVKPELSSTQALILAPTREIAIQSEEVIRTLASELPAPPVTSAVFVGGLPMLDDEKKLRRLCHVAVGTPGRVGALLQVERLVTSSLTLLVLDEVDSLLGDSFYEDVTWIYDQLPKRKQVLAFSATYTPELLADLEPLMRRPQRVMMCSETVSLLGVKQFYELVPAEVAELGSSQRSGDSGDAGDTARVAAGVAEPKAATTASATPAVFRRKVQQLLRLLGSVSFHQAAVFCNHKPRAEWLAEQLSAAGYPAAYLSGDRAQSERMEAMMAVRGFKLRVVVSTDVMARGVDLDRVNLVINLDLPYDAATYMHRVGRTGRFGSRGVCVSFVTAAEFALLVSYVDEQSAAATASAASAAAAAEANGGGTGGERQIGGAADQEAGSAKGHGGAAELLLPLPQVIPEDLYGYELESEWERQALQQLVKKERQARQQKQEKQQGPTSVVAQESNAAPALTSEARDGELADPLASQHLLDLPPLPEEIEAARRAEQAAQAAARKADKALRKQQHKVEEQEAAERQREQQHRAQEAEWRSYGDREYWNNWYGYNGYRYGYDGWPSEDAVANASRPFQSYNYSGSAPVPMHAPAYSGFHYGPHGASYPYQETSPAYDHQAGNGAYDTWGGTYGGSLAGQSQHAAAAAAWAQYYEQWGQAHGGSGAWGKGGWSSGWPQPGDSALYPPDVSNLKGAAADAAVPVHARGGLTSTGDSSGSVRDQQLQQIAAKARIAAAADRRDTGAGYSTSSDTAGAKESFDHTSAPALQRQRRPPNQGSADSGMDVSTEEAVSGSAEEEEEVGSEFSFGALRGEVVSRRAAGAVPDVSWCSGAAGRQVLESPGSAGIAIAPSGPVAMAAGGQVTDTSVAGLRGEDRADGEGREAGMGAGEDNDGELEDESWVFGDEDFSPDAAKVVDNLVVLLPPPQREQAQEQPSVGQGADNSPVSAPGAVPAAGSRQRWQQSHPPPAPVGPATATPHAGTTAGGSGDSAAPWRWPYYGSYDPYAAAAYGSHGWSQYEGQQLQQGYGYNGGDGGYGAAGWGCQEPSSFPGAAATQSYGPSVRPPPPPQPPQPHHPYAPHPGHHHQHHNNYYGHCYGTVLSSGTQGRYGQASYGSYSPYNQQPQPWYEGDVHPPLRSYRCAECLNRMLRDFVQRQYKWQEAYAAWYEQYKQWYDEWMQDYAGMATATAPAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4N6</accession>
    <name>A0ABQ5S4N6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Very-long-chain 3-oxoacyl-CoA synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11855" checksum="E06160C0B9803DEC" modified="2025-10-08" version="1">MIASLVRNVQDFSLNEFCKERAPKDELNYMWLGIYFMIYGFSTMYSTRLELVNDESKFIVVVYQIMMVTGVLKAIYPVWPPRIKHEITVQVAWYIVVFIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4V9</accession>
    <name>A0ABQ5S4V9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="458"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="631" mass="66741" checksum="12E89937EA6224FF" modified="2025-10-08" version="1">MAADPGHSGDVGAPYHLFLTRIPPKLPKEEVEAALSAELASRRIGVLAISVVPQSAWKNKGFGFVVLAGSPSASDIGAIAADLDNSIRVGGKAINVRVCQASVSGGKPSLSTGPLTSWDQPVEKITRLCAQVLHCYFARRTHQEAYKAEKLLALLSMSVAPSCCQEAKPVSLWGRDVYGSLEQMLLAIGESHFGGGWSLNGTIADRNAVLTIGKARHMQGWDALRDPLPIATLLRLAACKQRLESIHTPDTCSGFSTPTACRRCSDCGSLSAMHSTEQPSCSQSEASWSLRSLYTIPATAAENTSSESSPNLCTPNNDTEATGLSFPAPPTAGSLGTCNAMQSCSGNIAMADIALRPGHKQASTPDMLGSRAAAVASPLPPQPPSNRLTEAQQAGGGDAEDDDNTSTCTFVAEEMSTNTNTGKCESFPPGGTGRRGASALGRSAGTSGSRQRHRTPHSAHSSCSSCSSPCAPGPPSPPPPMPQEQPPQQQQQQQHNLVACGAPHQPSVLLVCAMSSSQLLGAHIAEPPSARDLLDRCARRLKSLIRRQPAQISYAVADLASYLSEAQEVLACCHDVPYFNLEGYASAVYGSFENFLLEISRLHLNDSWRFGGGHRTGEPRIMFRTRRLTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S505</accession>
    <name>A0ABQ5S505_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007826</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="129" mass="13809" checksum="9E804FE7AAA1C3B9" modified="2025-10-08" version="1" fragment="single">MHHHNSIIRGHILAQTHKAVLAPIRPHGTVPCQVRTKMVSLAATQVMGGPETRPAVQLSVSPGDVLANPDAVVQVGVGQPTYTVRVLYRSRGGPLEGASCVWAHIGHSGWRDTQDVMLSNIDTDGISHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S551</accession>
    <name>A0ABQ5S551_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NLE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008313</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF11">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="7">
        <location>
            <begin position="7"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="152"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="279"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="301" mass="32490" checksum="BCA1576A498D3E42" modified="2025-10-08" version="1" fragment="single">MAEETQVLVKFVTKLPAHLKVPEAPVAVPASLKRYGLSQIINHLLALDPPRPFDFLADGELIRKSLEQHLLSHNLSAESTLEVEYVPAVIPPQQKSSIPHDDCSLDASRAASSLSASPSSTRNAVGSGPIVSGSYDGQIRIWNGELQCTATVSAHPGGINCVRFLPQSQGDLLLTSGKDRTVKMWRVGGRSGGSGPSCQLLATYRGHQDSVEQVAASPSGRRFVSCGWDGRLLLWETGPRGARALCVGRCEESDAQRIRITAKDFLSLALPPSLPCDCPSRENTHARPEGRRYQLSHAKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S554</accession>
    <name>A0ABQ5S554_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Regulator of MON1-CCZ1 complex N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007734</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040371">
        <property type="entry name" value="RMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049040">
        <property type="entry name" value="RMC1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12897">
        <property type="entry name" value="COLON CANCER-ASSOCIATED PROTEIN MIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12897:SF4">
        <property type="entry name" value="REGULATOR OF MON1-CCZ1 COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21029">
        <property type="entry name" value="RMC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Regulator of MON1-CCZ1 complex N-terminal" evidence="2">
        <location>
            <begin position="68"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="537"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="660"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="666"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="830" mass="86869" checksum="85EDD4EDFBE0037D" modified="2025-10-08" version="1">MASFSYPGSAARPAAADALLPPSVDGRLGEEHSGLNGNGANGDGALYHVAALDQGLHLEPETLSYWGYDDGTNTLLVAKGGLVFAYDLSPGVVPGSPEQLRWTFPLADGPRVRALRLSLDGGLLAIQRSPVMLEFVELTTGNVFVQGTDRGRSEVLCFFFTELDGADVVLVTPTGLELLEFVPRRQGLRLRERLRMPVEWALYTHETRMVLLGTVAGGSPYIKAFQFVSSGLVALPPFTAGAAASALALMGPAAAGAAALVAGSPAAAAAVATPPACVAADSLRLLKVYGRVYCAHINRRSLKLELYRFYTDTVVLQHTYELFSPHVDLAVIDSVIVVHCTDAIAAAPLSGVAMLFDVVAPTLQPIANPLPLRPLRHGLPPEVGAMEAAISAGGTDGPERNGAGGGLKRRLEYLAPNVVLDRGTQIAYRLALNLPAVAESCSDPAVLVGFLQRRRGPIMPLPSPLGWPGAPPQPPHEQQQPKALLMAVVRNALQERMPMPTVRAIFEDLCAGYAAALDRELAMLAAAAPLPPAGMGSQAAVASGAAGGPSGASPAPPPLPPTAVPMPQYVTADELAQELFRWLHDEEVVDAPYLQAALWEYMSAAFAVGIPLPQYLQELSVEILRQQSQEGQVLQLLYSQADYVAPVVVERLLAAPNAPSRRHEAGAAGPSSSAGSAVASRETHAEGAVRASQPAGPLWRGPSLALDALSRAAMAARYEPVAMPSGGAASTAVSSGGVRGPGNGDAGAYVEALLAAGDVLRAARVMRRYRVSRPNVKEFIAAVGATGDEVALAAVYRTFQEELLASYPKYDLVVRKLLVGATTATAVPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5Q9</accession>
    <name>A0ABQ5S5Q9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008723</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050966">
        <property type="entry name" value="Glutamyl_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15462">
        <property type="entry name" value="SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15462:SF8">
        <property type="entry name" value="SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046537326" description="Serine protease" evidence="3">
        <location>
            <begin position="25"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="62464" checksum="49BB6E142FE8B63F" modified="2025-10-08" version="1">MDKKLFLCAFWLAAGIFCAAVVRASSNGCSSCPLTYAPVVDADTGLPFYNECVAKCQLGTGVNLVPAGSRRAAAAGRSASASSQPDFLKLVPAPNGTNAGIEVNAETILRFSNDGYKYVGRPGLIDMPAEGVKPAPSENVKKATNSAAEARANNNNNNNNGSQGSATNGPTVTLLVAYPQGDLYMKVWNQSELKALVKNHTGPKSETAQLSRATVPQATKNDGTTLPARRLLGSVIYGSDDRREVTSLAYPYTAVSFTSTGCTAAMVGATGRLALTAAQCVYNNGVNPSAPVGFYSNIIIAPGMYRGISSYGTHPVGLLQVLTTWSSQGLWEGNIALMQLSYHYKACGSLAYGYRCGLSGYTLNTAGYPADKSLYTMWTQTATVATDVCSGSAEKVFPFDAADGQVGSPFWTTSDNAVRFVLSRGMCCSGNGAVAITPSFYSIMTDFTTNYILTTPIYILVGGPSRNQFVRCDTSSWVCTGTTSRTSATRFYGHPVASTTKAAFRVPGTSLCLSLRSTTSTGNVNLYSCDTTAFNFYTNELWNIGRYNNIPVLLTDNGGFMSWGGSGVQMKTSPSCSSLTAGSCTWQVEAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5Y9</accession>
    <name>A0ABQ5S5Y9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008306</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046853757" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="33"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="274" mass="28778" checksum="5AFD77F1858FDB15" modified="2025-10-08" version="1" fragment="single">MRRVNVLLFALLLTVTTPAFARRGLLQSYLELFPPYGCNRDPAQSRFRVDPVYSVNGRQVCFTAQVMPCARPGSACCKGDVDFNKLELNVRPGCKGAISRVTVNGNRAPMPTFELYGAQEDKALYKMPGLNLTTANADRSVICVTLGSICPNMTTLCPQGDGSCHYSVIESGTCDCCPVGLVGIFPAPTIPAILLPPPPSPLPPSPPPSPLPPSPTPPSPLPPSPTPPSPMPPSPTPPSPPPPSPPPPSPRPPIPRPPSPLPPSPPPPSPPPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6J6</accession>
    <name>A0ABQ5S6J6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_008234</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF36">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="240" mass="25670" checksum="27B0112A8A01A553" modified="2025-10-08" version="1">MEGFLKAIGAGGARGPQKTQPSLLADWQDYSSRGTDVETGGGASTSSTSMLLSSAEQAGTKVTNFLSESFRTVQTGVTSGVNTVTSGEAFSIPSGQSLVYFFSFLAAGGVFLLLAFMLFLPVIILTPSKFALSFTLGCLSIMIGFMQLRGWKQQLQHMMSSERLPYSLGYVGSVAATLYAALVMRSYLLSLVCSGLQVVALLYYLMSYFPGGASGVKFMLGLFYQAAMRCFTSVYTMIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7M0</accession>
    <name>A0ABQ5S7M0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_009118</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ACC deaminase/D-cysteine desulfhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027278">
        <property type="entry name" value="ACCD_DCysDesulf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005966">
        <property type="entry name" value="D-Cys_desShydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01275">
        <property type="entry name" value="ACC_deam_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43780">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43780:SF2">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="4">
        <location>
            <begin position="95"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="456" mass="49287" checksum="FB13F4454BDF2AF0" modified="2025-10-08" version="1">MQRTFIPQSFHSTSIRQVTIFPDRVALQQLGLRSGRNLHLNQLPNIAMGGRQTKPATSPSAMLPASESFLQLQSYSPPDWASRILLAPTHRFRLGMLPTPIHEWQLPDLPVGVRLLIKRDDLSGMQMSGNKVRKLEFLMAAAVQGGYDSVITIGGIQSNHARATAVAARYLSFQCHLILRTSRQEVDSDPGLAGNLLVERMAGAQLHLVTKEEYGTVGSGALLEQLRLELEAKGRKPYIIPVGGSNSLGCWGYIEAINEIIQQTAYQQQPMKMTRGQGDGTGGITDIVMACGSGGTAAGLALGAALSPLNAKVHAYGVCDTPEYFYDYIDGLFEGLGVHPGGELLGGRSGARDLLRVVQARGSGYAISTEAELRTVQEVAAATGVVLDPVYSGKAIHALLEEIRADPDEWRGRTILFVHTGGLLGMYDKLDQLGPLVRTYGPPVSRMEVRHSTSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7Q8</accession>
    <name>A0ABQ5S7Q8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008912</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="203"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="582" mass="59439" checksum="0743D1032C2C4181" modified="2025-10-08" version="1" fragment="single">VSGDVLGMQDNGVLDSASGQRLKRLMREAFADLVEMRLRLEALEAAAAAAVEAGAAGEALVVPLEGFAVRPVSQVLASGVRLRGNAQVSGTMAVPFLAQLAAGERRPSGQGFEALRAGDISQDGGQAEDVLGAAAAAAVTASLQTDSSDRSSPDLSSWDALVAAAGLCPCPQLRLRIQSVWGQRLERDTTAAAATAAALAPLSQRGGAARGSEGAGSATSVEEGRPVAAHLQLVTEPGGRHLEVDKLLLKADTHPRLRLMLASQGSLENIGKNVNPFAAGIGQQRGLTTAMQRGHDLTLLSQGGSGAAVTAAAGDLSLAIGVFSGDSPTLSTAGAADEAMDSTANTSAATTTTAQLSLHRSRRLALSAIWSNTAAADASLGLGSEAAAAGLGCRGRRVVQRGLVAAAQLTEQLTAAAWVGDSLVPCCTSSSGLEGINALTVDCIGGGSGGPTDPDPWAFAAQQAPQREQQEQRMRWRERRVGLRLATLPDAETGRSLALCVSHRRHAEKAGVRNGLKYGFGYGSMLVTELSAAIPLAHGVMLTPGLVLLRRNAGSTSGVGEGPRGVASRTVLLAAAQTTWNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S949</accession>
    <name>A0ABQ5S949_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009728</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="74"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="180"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="118"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="260" mass="27750" checksum="96A3C9B7523B1414" modified="2025-10-08" version="1">MLSSGTSVMGPSSRCRPSTLTQRSVLIVRAASSKHLNFDTRISRRGMLIGLAGSLATVATQRLQPAFAETPVISTESINPPNPASAQSPWPSQPSAPQGASAPSPRQPDAPGPPSTSPPGSTITSNGNNGSGSSPSTSSSASKSSTTTTSNRITTLDDASLTDEAARRLMLEEEERRKRRKKSQGRLKELEEIRMELAEKELALLEKEKALLAKEQTLAVLTEELEVERKLRALLTKEKERAEEEAALAMGLCMGGFSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9A6</accession>
    <name>A0ABQ5S9A6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="20140" checksum="56B073223E3ECA2A" modified="2025-10-08" version="1">MNHRGCLPMCPPRMLRQLTPSPRKCRGVAQSDIRSSHARRARQPEAFRQHQQLHTKVGLNAMRPSPLCLAGPQLVLIDFWSATTATTITNATNAITAITAITATATAATTDTYATTATAATTATKETDAKIADTASIDAEPPRPPPQPSSPPPLRKQQRFTIRIETGKTLYKIESRWLHVYVDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9G9</accession>
    <name>A0ABQ5S9G9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_010056</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000030">
        <property type="protein sequence ID" value="GLI66294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1000.10">
        <property type="entry name" value="Guanylate-binding protein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036543">
        <property type="entry name" value="Guanylate-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48340">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1 (GBP1), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045201091" description="GB1/RHD3-type G domain-containing protein" evidence="7">
        <location>
            <begin position="31"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="558"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="596"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="8">
        <location>
            <begin position="62"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="627" mass="68674" checksum="29CC1AB822149AA5" modified="2025-10-08" version="1" fragment="single">MVGRVGGPSRSPFAWACALLSFLICRQVAAFESRPLPLVLPLESRSQLSVQREGLDLLNSIPGPVSPVVIIGPYRSGKSFTLNQLLGLPCNQGFGVGHTRTTETKGIWLWGKPVLRAGAGGAGAGLEGGGAGAGQQQQQEEARGGDDTVLLYVDTEGFESTGKANSYDDRIFALSAMMSSLLIYNLPETIRESDVAKLSFAVELARGFYDRSEQANTSGGGEVDGSGSDIISRSGDGGVEPGSMLWLIQRDFLHGQTADELVKSVLAPVDNPKHDTGLEALNQVRSSLSVLARNSTGFSLRQPHIDRARLCEIPDEQLDSTYTRQREELKELVKSLAQPRAIHGQPLTGPQLASLISRMVEALNEQEIPTATSLLAAFNRDVLTRCAANYTAALAAVRLPIEEDQLEAVELEARGAALEMLKVLQIGRQGAIDTRTELVKELERAYQVRRMENLAASNALCSELENKCEAELDELQNMRLPSLRKFDSSYQHCEKVFTDQCRGPAKTPNAERLEKAGTRARKAFGKDYNDRLFTGLLVTALAAAVVFRFFLRAQLLEMAAWAAFLFLEVYPKLHPVTSMYDTRGWALTVRMWEVCMAVLFAFKGSGVLWLALVVALAVMRMCYSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9I8</accession>
    <name>A0ABQ5S9I8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial substrate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_009829</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="24"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="141"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="234"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI66118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="33400" checksum="1255C5A345147755" modified="2025-10-08" version="1">MLAPASSPVGAAKAKAVLATTKKATVLQELIISGVSVGGATIVTNPLDVVKTRLQLHSSTGPALVRPGLFRTGINLVREEGAVSLMSGMSPAVARGFLYGGMRLGLYGPCRDALQAVADAPSSSNPTTSNAPLTAPRQGLAALGLKAGAGIISGAVAAGVTSPTELVKTRLQAKGCESSNAWAVMRQVVAEGGVLALWRGSVPSMARAALLTASQLATYDTVKRDVMKYSGWGDSLATHLTSSCFAGLVTTTVTNPADVIKTRMFVTGGSEARGITQTALGVVRSDGIRGFFKGWTANYARLGPQTVIIFLISETMRNALGLEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAH6</accession>
    <name>A0ABQ5SAH6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alcohol dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_010878</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the N-terminal section; belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000045">
        <property type="protein sequence ID" value="GLI66880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08178">
        <property type="entry name" value="AAD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07122">
        <property type="entry name" value="ALDH_F20_ACDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034789">
        <property type="entry name" value="AAD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012079">
        <property type="entry name" value="Bifunc_Ald-ADH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010378">
        <property type="entry name" value="PRK13805.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF83">
        <property type="entry name" value="HYDROXYACID-OXOACID TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00060">
        <property type="entry name" value="ADH_IRON_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="85"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="10">
        <location>
            <begin position="531"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="11">
        <location>
            <begin position="719"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI66880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="951" mass="102868" checksum="FD7DE05859372CBC" modified="2025-10-08" version="1">MSTNLLSCKRGVAPVFKPGTAVPLAPRFGGRRAPRIVCEAAAASPAPVAPKSEPAVVAAQAASATPAAPVKERPPVADDQLALLKTQLKRAQEAQHKYATFSQAQVDEIFRSAAEAANAARIPLAKMAVEETRMGVVEDKVVKNHFASEFIYNKYKHTKTCGIIEEDQAGGIQKVAEPVGVIAGIVPTTNPTSTAIFKALLALKTRNALVLCPHPRAAKSTIAAAKIVRDAAVAAGAPHDIISWVETPSLPVSQALMQAPEVSLILATGGPAMVRAAYSSGHPSLGVGAGNTPALIDETADIKMAVSSIMLSKTFDNGVICASEQSVVVVGKVYDEVRAEFIRRGAYFLNEEEKAKVRAGVLIDGRLNPNIVGQSIPKLAELFGIKVPQGTRVLIGEVEALGDSEPLSQEKLCPILAMYKAPTYEHAVHIAHSLIMYGGAGHTSVLYTNPLNKAHIELFESTVKTVRILINTPASQGAIGDLYNFHLDPSLTLGCGTWGSTSVSNNVGPQHLINIKTVTSRRENMLWFRVPPKIYFKGGCLELALRDLIGKKRAFIVTDKPLFDLGYADKVTRILDDINIHHQVFYHVTPDPTLACINAGLSEILEFKPDVIIAMGGGSPMDAAKIMWLMYECPETRFDGLAMRFMDIRKRVYEVPELGKKATMVCIPTTSGTGSEVTPFSVVTDERLGAKYPLADYALTPNMAIVDPQLVLNIPKKLTAWGGVDALTHALESYVSICATEYTRGLSREAIQLLFKYLPRAYSNGSNDYEAREKVHYAATIAGMAFANAFLGICHSMAHKLGAAFHVPHGLANAALISHVIRYNATDMPAKQAAFPQYQYPNAKHHYAELATMLSLGGTTDDEKVIKLIEAVEDLKKKVDIPPTIKEIFNDPAKDEEYIHRLDALAEDAFDDQCTGANPRYPLIADLKQILLDAHQGPILPIKSLEFYSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAR3</accession>
    <name>A0ABQ5SAR3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000034">
        <property type="protein sequence ID" value="GLI66553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="122" mass="12822" checksum="2C62AC4F7D58AD65" modified="2025-10-08" version="1" fragment="single">QVSDLISQLEKLRSGAAMMEARKGGLSGASTDLQGSSITAKFAIIPAASCPAMGATSPPSAVEVADAGAASIFINKAAQQPQELYQPLELTQPPKPHPKTPERRLPPEARNWGLQDCRSWAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB06</accession>
    <name>A0ABQ5SB06_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011272</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF21">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="31"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="625"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="936"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1032"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="517"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="641"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="656"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="675"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="944"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1032"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1341" mass="137497" checksum="2DA69381A4F47731" modified="2025-10-08" version="1" fragment="single">MAWTEPKTLKPSCATGGEAWLFEGFAVCGLALPLVSIPGGDVGSHLCEFHRFSPSLVDQLFINGRKAPQQLPTCCMPGGVEVISATRAGSRLELAAFQAPAVYSLVLTDGGGRRLYVTCLSYTEPLNPAITSRHEHLRGAHATRALCLISRLPYLATAEQVLRRLYTTVFRLGPSAPVGDLLRALLRLPSPATMHREVRFHFERQCFRVLPPDETGVPGGGETSLRALLIALSPVNVILSFLAVLLERRVVLVSRHLRLPTLAAEALLQLLWPFRYKHVYVPLVPASLLDLLEAPTPFLMGLGGWVGTSFAAEVAIGAGADGRGGGGWAGGGGGSGGCDGLAGAAAAARTHNEGLVVVDLDKDWVYECGELLPYLEHPAIAQLRCCLTALRKASDDMDCGGGGGGGGFSDWDGGYRSNRGRSGRSGGWNDLAFCDGTATATVTALAVGPAVDPWMPYIRVAAKRGWAPSHDALVRRLFLRLFSCLLRGYHEFLPAAVSAAAQGAERSSGGGGGGGSSNSSSTRGCSVSDPGSSSNGGGGAGGVGRTTRFSPSSSSMSWAFQVEGFLFRHCQTHGQRARPLPAQLVQTQGFYLLIDEYGGRDPYGWYRTACTACREGLEELAAAVSAEQDYPDTKRQQQQRQQRRKQRLRRAPGKGESQDDGLRDGWKENDDDGASDGGGGGGGFETAGAADGRNDGCSTGAWKAAAAAAAEPQVIIIIGAPAEDEDDGGAGSAANESAARHVQSYCKPCPLLQRRKAPVYRIFPSLHVSDLQYSSINFAADAVAVTSTSTPTAATAVTAAAAAGVASWQWNALDQPPSSDTAANRTPRDDAIATGAGAATTAGGGGEVGSASADISVSSSGAAGGLSSWKLPLTVNRRNRSLSGSLSGDMAPSLVGVGVGEAISGKPRYRISSCEVSTSAAAATTTTACTAAVSTPTATTTTTTGGGGSGRGGGMHPSQMPASSPAISEMPSARKSAAAAVVAEASSISVSFTNRWTSLSHTLRVGRKGTPPVTTLAAASLPAAAVPRASSLTTATGGAASTTSAPPASQGQGMGPNGNGPYHKGSNDHNRHNEYEVMSVAEQELVRLQIAALTQLLSWGEVVPPLLPSTPTPQQQQQNEVSCGKTAVAAAGTAAAVAATSSGASTLAAAELSSRFGGVPGEAEVERLRTLLCLQVGSNGGILIDYLQKEAATSEARRQAVAAAANDGAITGANAVTSSIPMPSNGRIGAAFASGGGGSGGRSLHPVAFSVLADAFDAIVNAAVQQQVFAVLTATLELSMKLYTSAGSGSAVRLLHALSSNPYLYTCYIWGGLLSHQLRSAAAGEAATTATASTPSRGSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB10</accession>
    <name>A0ABQ5SB10_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="146" mass="15713" checksum="B561E09A6EA0433B" modified="2025-10-08" version="1" fragment="single">WLPLDLVHCDDPRVGPALHRAFGRHMIAADDAAAAVLARCGLSSVTLTGSVSHRGVLTGGWDGGGGGSVAGVRLLLERLWELTEIVRLEERLSVRLAQQQRRAAAAERRQVVAEAEALEAVAVDEEIQAAERQIQDLDEQLVAAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBG3</accession>
    <name>A0ABQ5SBG3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carboxypeptidase</fullName>
            <ecNumber evidence="2">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000045">
        <property type="protein sequence ID" value="GLI66875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF254">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044978822" description="Carboxypeptidase" evidence="2">
        <location>
            <begin position="22"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="601" mass="65105" checksum="EED3389FCDCA87F9" modified="2025-10-08" version="1">MAKIFSKIILAIFLLASGSLAARFVTRSVEKAEVGAKSRHNGLTPEAAADRIVFLPGFQNALPSNHFGGYITVDANRGRRLFYYFVESERDPVNDPVVLWLNGGPGCSSFDGFVYEHGPFTYSLIHKPGSRGVEVELRSNPFAWNKVANMIFLDSPAGVGLSYSESTSDYLVTDTRTAADADRFLREWFRRFPQYRDNDFYVSGESYAGIYVPNLVRQVLVGNEEGEEPRVNIVGYLVGNGCTDERYDGNAHPPYAAGKSLLPWRGFKELEAECGGGEYWNKTSGSTCDKLWNKLAANLGALNVYDTLEYCFHDGVDDASVILPATDRANRGAALRARLSGQTAAAAAAAASLENGLGMGSRARGGSGGSGGGVSRRRAISTADLTQQLQEAGLLGVWPLAGGVVRPGPVLNWANVSAKLGGRLGGTPPCTDSRLCDPAQPRRPSLSHTPICTDSRAADLWLNDPRVREAIHAAPTDDIGYWTLCSDKISYMRDHGSMIPIHINNTKGHGLRALIYSGDHDMAVPHTGSEAWTGDLGFPVKAPWQPWFVADDQVAGYVVEYGHGLTYATVKGAGHMVPETNPREALAMFQRFIDGENLASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCA0</accession>
    <name>A0ABQ5SCA0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="5">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_011763</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008854">
        <property type="entry name" value="PRK11892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF96">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="6">
        <location>
            <begin position="35"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="359" mass="38712" checksum="533CCF65ADB41A15" modified="2025-10-08" version="1">MLPGLLQQARKHGVLCRVPRLTAAIRGFASAQSEMTVRDALNSALDEELARDDKVYILGEEVGEYQGAYKITRGLLQKYGADRVKDTPITEAGFTGIAVGSAFAGLKPVCEFMTWNFAMQAIDQIINSAAKTLYMSAGKISCPIVFRGPNGAAAGVAAQHSQCFAAWYSSVPGLKVLAPYDSEDARGLLKAAIRDPDPVVFLENEIMYGQAFPVNAQVLDKDFTLPIGKAKVMREGKHVTLVSFSKMVGYCLKAADHLSKEGVHCEVINLRTIKPLDKATLLASIKKTHKMVTVEEGWPQCGVGSEISALMQELAFDELDAPVLRVTGAEVPMPYAANLEAAALPQVDDIIKAVKKVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCE2</accession>
    <name>A0ABQ5SCE2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010829</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000044">
        <property type="protein sequence ID" value="GLI66846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046968703" evidence="2">
        <location>
            <begin position="17"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="384" mass="40638" checksum="FD01BEE628862236" modified="2025-10-08" version="1" fragment="single">MFWGWMFGLHLVPLLGSYGRRTTLPSDARGETQHAAGTSASAVTATSSVAASQGGESERGRQLLGSGGGRVALVCLQFSDPPVRRAQLVGGRELVSQLGEALEPGALVWIQSDVPYIASSFRKLFWRHGGFAPSARHAATPGSETTQMRHRIAVAAVLPGHISKSPKRNDQCETLQGDATEDVVVSEAATTVAAVATASVAAEGLPLLLLEADLETHEEPWLTEPQVAAVVAKHRRSRPLENGFRKGAANGQRGFECCKASSDNLQQQPGKHGHHQQQEEQQQRGCAGGMQEREHEQNGGGMDVYDITNAISGDSEEAEGIAGGVGDVSGSSRDGDGAGDCSDGGRNYDIGVDDGDEQDGWQGLPWLASNPLGVPTEREVYVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCH6</accession>
    <name>A0ABQ5SCH6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Galactose oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="409" mass="42960" checksum="2D6E165B2988FAC3" modified="2025-10-08" version="1" fragment="single">HWQPVMPLCLGQSDVIEAVPRWGHASAVHGHCIYLLGGYGGGGAHARRGDVLVYDTHRRTWEALDVEGVAPSPRMGHTAACIGDGYVVLIGGRTSPVDALEDVWILDIEARSWTQLHPSVDSNTTSGAPGSHSSFPGRYRHSAVSVPPPQAVSGTATGAAPTAAAGAATTTCSRRHSLNGWRVVVFGGRNGDAVLGDMWVLSRDPWVSGSASLASGWRWQQVARAAGPGIDMPSPRKSHAACWVPASTDPPSAAAAAATTAATAGRMYVHGGTCGYGLHFDDTFYLDLDSYTWYRCEPRHDSGGGINGGTAAAVHPPACFSHTLSRWGPLLLLVGGYPTDHHRQLCVLDTRSHSWANLDTVMQTEPAVTAAVTPAGSGGATATSPPPFPVDFVPIRHCAEVLGDTLYVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD11</accession>
    <name>A0ABQ5SD11_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Solute carrier family 40 member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011542</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in iron transport and iron homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009716">
        <property type="entry name" value="Ferroportin-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660:SF57">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06963">
        <property type="entry name" value="FPN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="457"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="494"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="624"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="677" mass="71137" checksum="1D8438E76872C280" modified="2025-10-08" version="1">MPPPCDLPSEKTASGVPSRAKVFLCSSYALAAWAWRSWEFIVALILIELYPDSLLMVSAYGILDNLARVLLGPAVGSYLDRHERLPGAYAMLRLQNLCIGGSAVAALVLLWPGSNAVRFKLLYWSLMWLLTIMGAASSSGSTGVSISVEREAVKALCGSDANALAALNSVMRAIDLSALLCAPLVAGMLMTVTRRSFTAAAVMAAYCCFAYVPEVLLLEAAFNAAPVLRRQKVPVAGDDGNATGDGPVDSSRCDEEADERTGLLAEYTDGTDGTRVRVAGFKATDAEAKSCFGCPAGEGAVKEGLTGEAQIGARQVKRAPGPGSEEDDDNDMGLGVLASSRLSQPLLLDAVELLPTSISSSCPDGGDGVDGTSSPAATTCRRADPQLPFAVSEGGKRSSDTSLTVLAMAAPTTLFAPGSSAAPVVGYTTRLRTMYRSAARQLGLYFDSWRVYWNQPVLLLCLALALLYMTVLSLGFLMTSFLKWSGLSEVEVSAYRGIGALTGLAATAIFPPLLARAGLRFCAIAGVTYQLTCLAAGVLPVVVVAMAHGGDLRPSVTQVRVLVAGLVSSRTGLWLYDLAVTQLIQEDVRQNQLGTVYGVQSSLQASFEMLSFVAGLINPRPARFYWLMLGSLGSVATAATMVWCYGYLRRRRGGASDGGGCVSSDAARATNGGNDAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDZ8</accession>
    <name>A0ABQ5SDZ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011830</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16619">
        <property type="entry name" value="mRING-HC-C4C4_TRIM37_C-VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="16"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="7">
        <location>
            <begin position="102"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="SIAH-type" evidence="8">
        <location>
            <begin position="137"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="4">
        <location>
            <begin position="102"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="250"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="328"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="430"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="778"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="944"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1297"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1512"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1603"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1688"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="476"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="782"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="865"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="876"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="902"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="948"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="965"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1031"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1061"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1519"/>
            <end position="1530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1770" mass="186691" checksum="8122514AAB2BBB44" modified="2025-10-08" version="1">MDEFQYAQEVPADLLCSVCYHPLCDPRCCPRCQKAFCSGCITTWASTQKRKDLPLSCPYCRAKLRLSQLFRAEALSVRLGDLHVLCPYDCSGTIRRADLANHLACLCPRLPVPCPDCGVSVFRGELAAHVGAPSCHNRRLACPNQPLGCQAQFQLLDMQYHAMQCPYEPLECRHCHATVLRGQAEDHCWVACPALCPCPNRCFGCAYVAPSLEHLADHVVRNCQYAESSTYMARMCGALGTGDPMYGGGGRFRSKLSSGGHSHLPTPHPQFPHLDERRSSHETHRSSTAGRLCGMRESAPGSAAAGGVWCDGLFVPASLYPNGLLEPQSRLLPPGGPASGNGGGGESSSGSAKAPAPWPWATDSTGEVSDAATVALTKHFVPAPNADQLRQNLQLRRQEQHQQQIWEAKAASVVVPERYRRLLRLVRGEEEISEEDPRQGEHQPQMDVDVSGQDRGLESAGEAVTGGDLIMQDAVGGSGSGSGSGSVGSGARGPALKLELELPPPEAFWWHPVATADGPTHGSETPPLSKTAVGGHEDLDPASTSAPVFHIVESVDTSAHADHLTLSSATVYGTLIDLMARLYERGGVDGSGGGGTTAVLYGIDNEIPRPEAGRRDFQLLQLYEGNDNNIIDLPSCDTALHHGHRAFWALGLLRLTRRLWPSASLHWRSLAALNDLVTALARNLLGRVVEVISCELVQESSPMGEGDIILAVQNLFPSPLTAEALESIRQVLQMYDDIFFESEIWLEGWSTLRVKLELGLDLGRVACLVGQYTPLHGHFVAGEDDPESEEGEEARDDDSDDSDDSDSDDSDDDDDDDDDDDGDDGDMEEDEESSEEEWASDSTNDNDSDNGDIEEVPRRRTLATNDRRSRPRRSDGSSSNGDGSLPPLEHPDPEGPRGHRAGGSTAAASSRNAGGSVNNGGIRAPVMGLLNNARLRFQSLRLDLRAFGRGGRGGGRGRPNAWGSDDSDSSSDEWESMSEEEAVELLQQQQQQQEERQDGQQQSTSHPPGGDAAGREGRPATIRPPDRIYGISGGGGRGEGRGGGGGGSASVDSPSEMDVASSGSSDSSDSSESRADIRGVVALTAVLDWFVMKLVEKTRSYVESGLMQLGEGNQVQPWHLQLVLDTSLLGQLLLLPRERMAAGNGVSTGVSRFKESGFCDGPSLGSQIIGALPLRPLARMLASLALQPVSALLVLATTGAAATATGPAPTPITEGNGGSGSDVAQPTPILSPLDMRCYCGGPARAAVASGPPCSYWSGHQCEGCAAASAGVRQLPFDERLKIVGGVLWGAFRGASGKGEEEVTTGEQALPGSEGPSPALPCVLHPPPDLVAALTGGRLRGELLQRLLPPALRPLLQPEAFSYAARLANLGLTSGLVRIHQLDTPCWWRQPGGTAYLAASAAVEPQGQNTTSVLAAAISTELAAAVRPDPAWLHQLQPHLGMIRDVEAAAALCRDVSAVLYGMPTQHVDRITVQLNASDGVDGAFGKEADAASLTDKSSSSGHHLLATTVADAGSAAAGGKEDNTRPDINHAEATNQSQMVGVSGCSPDRTSGSLVRGSPSLHELPICIPRDVFMAMFHESWEVVCAEHGDGGASAAVAHGAPLSGAPEPSARVHAQQPRGHPGGAGQHRRKRGRPVFPPAMGAVSNSAVRRHFRPLQTADGESPVPIARVARPSDGPGPSAPVTTASVFGPTELGRGPGQGSLPDEAKGGVGEEMEWSPDALELLHKAAEAALAEAIQEDGEGGISGRGGYEATAGKSKIAGGGILGNRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEC2</accession>
    <name>A0ABQ5SEC2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="169" mass="17059" checksum="D2C47C1F0E978704" modified="2025-10-08" version="1" fragment="single">LDELAAAQVQLQHLLPLDLTGGDPAAAQLQLAGIMQQALQVVEQQQALSQGLHGLQPAELAAQQSALLEQAMALAALGPLACTMPSMDAHGHPTFQQVPDAGAAAAAAAAAAAVAAAAAASVPPSVIVAQQQQQQMCMAHQQSGRRDRRRKGGDGDGAEGGRVGGDKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEF8</accession>
    <name>A0ABQ5SEF8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-related protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10210">
        <property type="entry name" value="ASKHA_NBD_Arp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.70">
        <property type="entry name" value="Actin, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="46627" checksum="86F59AE2448FA0CD" modified="2025-10-08" version="1">MSIPPVVAIDNGGGNCKIGMAGQPSPARVFPNASAKPKGERQTLIADLLADTKEVSQLQLKRPFDRGYLCNLELQRDIWSRAVRTIVRRTPGECGLLLTEPLFNFDEIRAGTQQVVFEEMGFRSFYAAPAPLFSMHAAAGLLGASLPAARTGAGVVVDAGFSFTHVVTFFKGQVLESGVRRINLGGKAMTNYLKELLSYRSLNMMDETYLMDMVKEQLCFVSQDVKADLRMAQSAARSPHRREFVLPDGVHNLRGYLRDPAEWAQKVESIKQAVRVAESIDEKKRLVAQLEADQVLVLNNERFMVPELLFHPDDIGLHQAGVVEAVVQAVTGTHPALAPLLYGNVILTGGVASCPGFKDRFSRELRAMVPDDHQLNVFQPQAPALFAWEGMSMFGSSHLYNKVAVTKAQYEESGSWAANRQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF04</accession>
    <name>A0ABQ5SF04_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="119" mass="13636" checksum="FE0172193DF67B71" modified="2025-10-08" version="1" fragment="single">PNNCLYTLFTRMSGALRALGLQALRLNLHSALRSQQQPLRKNFADYAKPKVDTSLDHVFGDNSNKLAYNFEPMPTGSLFFRTLVLTGVFMFPVWFMVYDEYKYTSFIEKLAVAKAKKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF33</accession>
    <name>A0ABQ5SF33_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012391</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="172"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="238"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="294"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="322" mass="34037" checksum="53AC2DCE4DFF25CA" modified="2025-10-08" version="1">MANKGPALPARKAAGRRTAAAHTRQLPIIEAQTVTLEDGTEVPIKIPPGMPAAQAQALVAYLKSNPSAAKAAYEQAQTIMHNPSLANAFTNMLAPQAPAMMERLNALKDDPELKDMFEDMKTNGAVAFQKYWDDTDLMLKISQKLRALDVNKEPAQAAPAAEGSAATSEAKKLITNLHDAAKYGDVEAATRLIDEGADVNALNDRGISALGVAVGFNRLEVVKLLIAKGADVTFRDPKKNTLMHYAAGYGRLAIAKALLAAGAELDARNDAGQTSVDVATVNKEHEMIRFLREKEAAAATAKPTESDAEAAAPEAAAEESAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFB8</accession>
    <name>A0ABQ5SFB8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bms1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_012370</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. TSR1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="6">
        <location>
            <begin position="18"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="360"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="429"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI68047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="845" mass="90153" checksum="4C551A3A73EC4E9D" modified="2025-10-08" version="1">MRDAKRQQLLEKKRRIAAPVVVAVLPLSADVDVSRFFHGLRHACEHGGSSINGDDADVETDILMSAGPLHPTTVHVAARPRVRLTILPPPSDRSDPLAVADLGRCAELLLLLLHGGESPAGGSGVDAAGQSAMAVMRAMGMPPVVCCVVGAAGSGSGAAAAAVAASMKERSAAKKRAEKALATHVAGEHRILHADTAADMAAVVRHLVEHPPAAPPLWRQQRPGIMVERAEYEPLPEESAAGPGPSAEFTASPGAGPGGTLVVYGYVRGAGLSANQLVTVPGGGDYRILSIHAPRDPNALGAGRPLHVAAKPMEAETEAEATAAGMEMDQGAASGDVGPGRLIAQPDPEEADQLVRENVPEPGAEEQTWPTDEEMAAATAPRTRRRKVPEGTSEYQAAWILDGEDYGDEEEEEEEGEEEDFGDDPYGMDADGASTSGGRAAGRQRGDSGGGMQMDLEEGDEVAPELQTIPDDDDDAGTDAGDTAMLMDEEGNGEEGGGYVAALEAVKQARRARRGNLGSEQELAQLEFPDEVDVPLNTMASVRFQKYRGLKSLRTSAWDPKESLPAEYARVFAFENFKRAHKRAREAQQRTTQDLDPCGVSSGSYVAIRIGGVSPSAAARVTSHVDAAAAGSVVPLTVFGLLQHEAKLSVVNFALRKATGYTAPVANKEELLFVTGLRSFTARPVLSTDDPGLDKHRTEKFLHAGQQAMASVYAPIMYPPLPLLAFKLAPPGKVAQLAALGRLSSCDPDRINLKRIILTGIPSRVHRRRATVRFMFHNPDDVRWFKPVELFTKYGRRGRITEPLGTHGAMKCLFDSPLQQRDTVCMALYKRAFPVWPKDMGFAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFE2</accession>
    <name>A0ABQ5SFE2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="420" mass="44346" checksum="CC1B21CFCECC91FA" modified="2025-10-08" version="1">MDVLLGLRVKVPATEWGLQEGAFWGVIVQLGIDTSGSSCVGIQFDGRDLFHWPTDMVIEWLADSILMFKRPRLGAPDKTILRPTASQREPAPWMGAENVLRHASEHNTGQRCKGIPPDDPRASPPAALGLVHTSNTSIGTADAERERDCSGIVDPIGQVPNDGVECGAASDEVRIGRAFEPTAQESLRTCSRSTSSNSSGGICAAGRGLMADASSSSLQGPASEDLSADRLEVESAGAADMISDAGDMPGSARGDAGCGARGQAVGCPARNKQRRDYHSVGVELIERLTAQHSAGSSGIVPWTAANQDIQDPRVADQSCEGHDLMQESAPQVPAALERCQMSPRLAPSALMERSRTQLLQVMPPSAQLPNPVNQLGRLSIKPERRSAGDGTKASLARIGNLPKNGMSALAAGFPLGYHMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFT4</accession>
    <name>A0ABQ5SFT4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042859">
        <property type="entry name" value="NOL11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15633">
        <property type="entry name" value="NUCLEOLAR PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15633:SF2">
        <property type="entry name" value="NUCLEOLAR PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="841" mass="86979" checksum="B56B99B9A58DA771" modified="2025-10-08" version="1">MAAATLLCRQAELLGATFNRSSGCVLVTIQGDGIQTYDLAQRQLGSIVLGSRSGLKFQVASVQHHERAQPSGEGPSSRVVASVVHDREAKNHSLCLSDLGGAARPLSDSPSRVALPGATQSCHFLCQHNVQHHATAAVASTRRLAAAVVFTDGRVGITDVGPAGSAGPLHVLPCGQEPAPHHVLATSLNDTLLVAFQSTAGSVATLRVLRLSSATAGIAGGLTQFKTLTLPCPGASGAAPANTFITGLTMNSAFALVQWSNGHATVVHGIQGSAGTLPLRPCTFPLADVLTNGASTSTSAPATTPVATTSSRKRKNAAAASSISAASGVRPRVLCAAMDERQFVVLDLESSAAQSQVCFSLHDAHFGCPLLSGTVEVDAPLLSLDVSQCAVVAAGPEGTIIRLGTSLHVLHIQAQPVSLLSLVARLSVSAAGGTAAGEAQVKIKPCGFGAAAAAKSLAALPAPVSLDPALLAGQALGTLTAAVRTDVPDDDVQDIVLEPVPTAAVSHLEDICNSLIEQLLAAADTPPPQALLDSAVSYVAGRRQHGMAVPPQLLSLATRGLAAAKQWNQLQALLRHVPEAGLNGCSDVLVAAVMDQQYFLLTRLATCLDEIEAGALVDTLQLLLAPTTRTNLTARQRYYKTLRGLAEAQISGAEQAVTASGAKLPSPGAFTDTTAQLAKAQCAAAVVDGFSYREVALHPLIAIPVDAPAVQVGLRRLSAPLTEALLAYLAKWVRKYTDGAIGEVANLVMLPEGLVFPFYHQVLEWTRMTIDAHLTRLLTAPKSLPALESIEAALNSAVEDNKKLVMLRGALEHVNAAAPLPAAHIAVATQYTAEVLDLRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG24</accession>
    <name>A0ABQ5SG24_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Generic methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012488</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1580.10">
        <property type="entry name" value="paralog of FGE (formylglycine-generating enzyme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027625">
        <property type="entry name" value="OvoA_Cterm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027577">
        <property type="entry name" value="OvoA_Nterm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051043">
        <property type="entry name" value="Sulfatase_Mod_Factor_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005532">
        <property type="entry name" value="SUMF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042095">
        <property type="entry name" value="SUMF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04345">
        <property type="entry name" value="ovoA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04344">
        <property type="entry name" value="ovoA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23150:SF26">
        <property type="entry name" value="GENERIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23150">
        <property type="entry name" value="SULFATASE MODIFYING FACTOR 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03781">
        <property type="entry name" value="FGE-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase-modifying factor enzyme-like" evidence="1">
        <location>
            <begin position="267"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase type 11" evidence="2">
        <location>
            <begin position="714"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="916" mass="102561" checksum="3198895CE390B2D2" modified="2025-10-08" version="1">MSTSHRVDMGACRNPSLNIRPDLARAATAITGPSLDDELVGPRGTWWWTGKAPADCPGFDKAAGVLRSLPLPNLRSFTRQSVLDYFDNLWTLTEVLFASLQMTDAFIRQPYHQLRHPMMFYYGHPAVLYINKFRVAGLLDKGINQYFEQLFETGVDEMSWDDLSRGRDDWPPVREVHQYRKQAYAVVRDVILNHPALDQAQVGWDDPAWAIFMGFEHERIHIETSSVLIRELPLTSVRKPEFWPDYHPSTSNSSPPVPTEGVDFPVNSMVPVAGDKVVLGKDITYPSFGWDNEYGRKEIQVQGFRATKYKVTNGEFLRFVKASGYMNPKYWSAEGWGWKTFRNVKWPTFWMPDGPQGLHRYKLRVLFDAVDMRWDWPVDVNYHEARAFAAWRTEQDSSSVRYRLITEAEHNLIRNTRDRVDAHLLIAADHPAAAVEPNRRPSANEHVSPDAAMLASGTDALKSSACGFNIQLAYGSQNPVTELPPSEKGFHDTFGSAWEWGEDHFAGYAGFKVHPYYEDFSAPCFAGKHQLIFGGSFISTGQLASKFARYQFRPHFFQHATFRLVKPDVDMSLYDSARYGSQNPVTPFYETSCMDAAPPHMGDGPCCSVSRRAAFTPTQAEEVASVKAKLEAAQVAYESDAILAQYLSLHYGPLDRVYPDLLHEVGMIADALDFPRKLAECLTAWADRCGVLTSEEAKRMSASGRDAAVGPRALDLGCAVGRSSFELARRFCQVVGVDISKTFIEVAAKIRDNGRMDYDCALEGEITERLTAALPAPSQAELHPIEAGRCRFLQGDACDLPSALGQFDAVLAANLLCRVPEPSACLAQIKASLRPGGVALITSPFSWLEQYTDRSNWLGGGYKDGLPNRSADVLKSLLAPDFEILEEGSMPLIIREHSRKYQLISAHKLVVQRKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH70</accession>
    <name>A0ABQ5SH70_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013878</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="3">
        <location>
            <begin position="102"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="CBM20" evidence="3">
        <location>
            <begin position="296"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="429"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="627" mass="67029" checksum="3230D92D0D1A1734" modified="2025-10-08" version="1">MSSAMLRRKSLRASNALAARRPKVSCPVALKDAAQTRRYVLAHDAKASCTKNVVGCRVRPSTAQLHALQRCRPLSSRPATSLHASAPEGSASSSSSSASSATATEAALTITTKVVVPYHLTSHGQQLLLVGSCEALGNWDVKKGVRFTWCEGHSHLAEVELPIHTSIACKLVVQGNNDDAIWEPEANRELLLAPSTLASRAAGYTVLCHWGYPECTQIMANSLRRGGAAASGSGSGNKMNGHNLRSSTAGGDNSILAGVKKALAHASSRGVAVIPGIVSSWEGEGGAVAEEEEAEDAAPVQCQVTVFVPKAGPKLKPEQSLVLVGSSTALGCWAPENGLPLERAGEDSPMWIAQAELPLGEGLQAKVVVVDSLTGAALVWEPCENRSLARFPGGARPVMISAYWGTAPTHCLEVDRLSSTAAATNPQVVQMLRQQLAATATQLDGLRRERDEARKAVATSEEQMRSLEGEVKSLGRSGHQGRQEMVQLAEQLQTARRLYEFTKKEALELGGTVGVTRQLCSAAKRELVGLATQLDSAKRAYEALAPQVERMERQLASTNKAFEATRPELLALEAQLERARTLFDRTLQKIEAAKALTTSRELETVAVRRRGAETVNRVAREIQFAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHG9</accession>
    <name>A0ABQ5SHG9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="2">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013398</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SARM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46270">
        <property type="entry name" value="ARMADILLO-TYPE FOLD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46270:SF2">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="2328"/>
            <end position="2403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="343"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="403"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="467"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="807"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1169"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1379"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1470"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1512"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1561"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1670"/>
            <end position="1722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1738"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2043"/>
            <end position="2073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2144"/>
            <end position="2175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2220"/>
            <end position="2266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="349"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="403"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="418"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="504"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="530"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="810"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="841"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="855"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1182"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1399"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1604"/>
            <end position="1615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1616"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1684"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1753"/>
            <end position="1780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1781"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="2238"/>
            <end position="2249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="2254"/>
            <end position="2264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2403" mass="250072" checksum="BDE31868822B2323" modified="2025-10-08" version="1">MPDRDASDGGCGGGSMMANGFIHASPLHLPPQLSLCESASPEAITGAVVGSIVPSPAGAAHPDAPVILSQEQQSMAACAAVALRNLCHQIANHPGLLAAGAALELAAVMQREPDPYRRINATMAVALLVGHEEQHPLMRMDEAGIREMLMVLACSRAKVMCHGYFWTCWKVCQALARLSANEDNKQVLTREGGLEVLADVLLSEEHSRKAITVKYCVEALWNLAFYESARKQILANTALVEAIRAARQSSLDLVREVARGCLFTLGIMDASTRRTTGVLQHPQHHHLHHHPHEQAGFDARELQRVFSLADSTAATATTTIGAAAGGVGGISVVDGQEEMQLSGDIPPQRHPHQHQHQHQCHHHNDYQNHRQHGQYDEEQQQELQQMRTLESSISVVYEELAPSAQVPVPPATASVSVRGGGGSGSGSGSGGGSGGGSGGGSGGGGGYHQVAAGPVAVTSLRRTETDALGGHLCGPTTEWAPPAYPGSQTPTSPISGLENMPLPGNSNGYTEASQQQQQAPRDAPPGVLQRSTSCTSRYRSSAGNGHQVQGAPDSPTSQFRGHGVGGYGNGAGVGHSDRYSYGYIPYSFSQHNHLAQHQHFQHPHQGMLGYHHSRSVATELSGHGNGYVGLSPVVSLGMGSGSGEVSVQGAAHWQLSAAGAGVGGVHATGGDCGHIMISYEWGSQQKALMIKEALERRGLQTWMDVEKVSGSTLEAMALAVEGATAVLLCITKRYKESQACRAEAEYAFQQRKRIIPIMMERGYRPTGWLGILIGTKLYFDFSERRLINERVAALERELGPLARLCRRPGSTASVSRSRPPSSQVASCTGPNSQQHPHHLHGTGQQNGNSGHQSQYRTQPGTRAGGHTTSRPPSGTHSVSQRSELPSPSLSSYQRQASQARRGSGASYVLPETPFDISVAPDLSPGAGTGSEAAAAEVTASAVAEGPRYLTLPGRNSFARGGSMTGYCGGDGGGGGGVALSSIGAVADPLQAARAASVAGGGRGSPVPISMMPELASPEPPQLEDPDSEAAFFGSRGGGSSGGGMDLMGPLCLPPLLPTAPLSWPAPGSGAQAEDGSTVAPGLKPPATLRPTFGSVHQPELTGSPAAALDDSQVEELGAAVSGPAIPLSDIGTSCSPALLMQAQGPQIHHHAHHHHHHGHLMAMVSHSPAIPHSQTQPYPHAGHGHGHGHGHAHTHTHGHAHGVSHMHPDWRPHAPGSDSVDASAITDGTTAGAAGAAAAAVRQHPMGMQLAVPVTSSLSACDCGGKDGDVGDMRVAEKGGVAVELGTDPLLLHLPPAIGLHRLREGPVHTVAMAMNEGAGGRSEGTDGELSLLGMSGTPQRFDPLRDLPFGSGYEERTQGQGHHSRHHYHNLVHHRHGEHVVQQGQLQGREKLAHHQQQHQQGEGQQQGTGHPQQHWQQQERGEEGEEDIVPGLHRTSCAVPFSADDIKDGASVPSIVVPDPCVPGRSAAGVRWSEDNPTTASPSRHRLLRDNTSTSLGFTSEYAASLFPGAAGAPSENDSGMAAAVDERSEPSRSPTLLLRDLGEITAAQLSLVGNDAVDRTGTEAGKAPVTERGAALEAGHEDKVGPVMGCAGGEMRCPAQAREKEQQKQHREDEEDEEEEEEEQGLQQRGDVLAPLPGRLPLARHSLQRQNDGGDRKAGAALLHSISGGPLESVDDEAVSTDGQSSNTPQPGGSTGDLLGLPGGSSAAEQFVQALHPRPSDDVAIAAAGASAKDPIPVVQSLPPPPEKPPLQQQQQQQQQQQQQEGQQQQQQKQQQPAATQSLGPSTLASAAARSPFLTLLRPVTAALVSAAVALAPPSGGSRSGWISVLERTDTVTAVPTPFTRGKGSNLDDALESAGARSVDNAPQAAFGSPLVSALVLPRVSTSAGGAAGAAAQPGTPTGDDRVGNETCGSCAPAEASVSTFMPRLPTADGAPATTSGLAALRYKAMTWASRLAAVDVVAVSSRADTSRRTSCVGRAEGVAPAAATPVTTGVDGDADDLVGERLAMAMDSGSRINIGRTRMSIDLASATRSVPCARLCSSSRSQGQGPGPLPPPYPASISGEGSLLHRKGGPDVNACAVGAEIALSVAPGGGLKEGVAQMEELATLVDPARRSGSASVGGAPAPNLSRVASRRSAGFTAGELSGRGGGSGSRTSIGSGTAPSPELRITRRWSRHSEITGWDYPPGGGVVVEEAAEDSERRGDYALRREGSLQRRQRLLRGESLVPEEPGNDSPSGGFSSRPSGLQGMEGNSTGGQVQPTDAAHIPVDDLASDNFVSQSSSMGLDMDMEMEMAGGPMEMGMRMEVETEVVVPPSSTSQLVERWKAPQVRDWLVRIGHGGLADRFEEMGITGRALCGLMRVMRGGGGAAVVRDMLSNELGVTSLAAQLELLEELHRLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIQ5</accession>
    <name>A0ABQ5SIQ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013807</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029760">
        <property type="entry name" value="GPX_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
        <property type="entry name" value="GLUTATHIONE_PEROXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="47"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="207" mass="22522" checksum="A20D5CADC39A0EE9" modified="2025-10-08" version="1" fragment="single">FIYTTKMMLQSRSIHTRPAARVAARPARVYAVKAQALFGFGSAKTAELATSEFYDFTVKDIDGKEFKLSTLKGKAVLVVNLASQCGFTPQYSELQGLYAKFGKQGFTVLGFPCNQFGAQEPGSNQSIKSFARSNYGVTFPLMSKVDVNGPGAEPLFNWLKAQKGGLMGDDIKWNFSKFLVDKEGAVVGRYASTASPSSLEADIRKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ16</accession>
    <name>A0ABQ5SJ16_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="444" mass="47508" checksum="45FBBBA20C6C6133" modified="2025-10-08" version="1">MGQTLTTVPGYRPISSSASIPDNKMYFGHMNAIQRPCGRNYEGRGNGLAGILHDTSNSRSRESDVFGITYIRNIRCGASSTHFSSDVPSGAAREERMANNRAGSLTPSNIPTNLVHSPDRKHEADQQSQQVPTSSLARKPSLPLPPPAASARQGAVSKRSGRPKGSVRVRPTAASCLSVPRGVVAHSAQGKGWSLARQGCSKNSGWDHVPAAERRRSRGTDATLAHNAMMMPQSMIHTFAEPAGMTTATTMAMVMVDQSPHGLPPTLPMLPPLYNFGSLPLSGRRNWDGVVAQALTEGMDLLGTSVPTRDGIIPFMGLDRMATTLGAVDAMHGLRRSEVQASSFFQPLPYSLGSLTLDERVQWDAVIVEVLTEDLDAAHATVTLRTGTALYGGMCGAAAAAMKHVNKMEKVREPLPAPQRTYDLGSFTRSMRLCWEGIVGAVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKY6</accession>
    <name>A0ABQ5SKY6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="135" mass="15593" checksum="44D9F638D3C07749" modified="2025-10-08" version="1" fragment="single">YARATKRQSYHFKCKYGPGGMVSESDPRVSIFIGETLFFALIRPRCDVAPVKVAVCNLHRAEVLRPKELYRVKDYSRRDATVNEVRQEILLNYFPKEVGMIDSKVIRCRVNNELSYFLTYVTFSQLPTNDGGEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLM8</accession>
    <name>A0ABQ5SLM8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="3">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_015446</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="8">
        <location>
            <begin position="14"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="8">
        <location>
            <begin position="516"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="8">
        <location>
            <begin position="646"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="708" mass="74603" checksum="146E24112790A469" modified="2025-10-08" version="1">MSPIPRDREKLTRMEQAVRVLIQGLGEDLDREGLRDTPKRVAKALLDCTQGYHQDTSSTLGSAIFHEPIVHQGGEGVVVVRDIDFASSSEETLLPFHGRCHVAYRPKGGVVLGLSKLARLTKQYAKRLQTQERLAAAIALALQQSLDSYGVAVVLNARHLSNDASPEERTNACVSGDFASKGSPEFEELLALLDLEDLPQAAVAHLDSGGFHSTHMTQHIISSHAAAASAITRSDPGFPAPGTPDPSEKDADGDSDDLVLDLTPCSCEEMEAAVQRLLVEMGENPHRQGLAGAARRYVMSLLASTSGYHMQLPLTLLQRRLLEQPPQRLHGTRELERQGYTYGHAASRENRDLDGECLDGECHVGGGGLMVRTAATSGCVHEDYEMGTRSCNVLAGKGINSAEGSAAVGRAVTAVEAVAGSPVNDGALGAALSFARCRCPGDATACRSCGSPRAGTVSDTARTLGVVSATAAVAGDLAARCCMHSVCCRSQFNGQTAVSTEAVAVPLTAKPLGGAVILHLPFTSQCEHHMLPFYGNLMVAYLPPEGASVPITLPGGVVERVVLMFTQRLQVQERITHQVADAMQEVLQAAAAAAAAPLIMPGLGDRSACVGDECTFTGQHLQPSCNVTAVAKSGTGPVTGDGGEATAGVMVVCDAAHMCMVARGVENHSGSTTTFAMRGLFTTRPELRCAVLRLFREHQIKLAAARGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM56</accession>
    <name>A0ABQ5SM56_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4159 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003882">
        <property type="entry name" value="Pistil_extensin"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01218">
        <property type="entry name" value="PSTLEXTENSIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="62968" checksum="ECDD480FAA81F9DE" modified="2025-10-08" version="1" fragment="single">MSITTLHTSHRPTCSISHRFHAGGRRLSILVAHWIHLVLLMASTAHAGAPFNVYLYANLSVVADPARLAIISNYILRIAPGSRAIDITTQPTAHQLDGAAGYVVSANKASYFATEGILSNQQKVTDLATFVRRGGALVLVDGFDGQTNTFLPLLTAMLGTNSPDCVVVRINGTVRVRLRINVGYFSDLAPKLAAKQGQGASGIMCRSGRPIYSWYTTGRVQLGVAWYWSLGRGAIYWIGSGFLVPQLNGYEEVMGASLRTAADVLYSANVSQPPSSTNAPFLGQTPLAQKTQPPPPPPPPPPPPVLGVPTPAPTLLPRPSPRPPSLTPPWVAVYLPQRPSPARPSWSISASPSLPPQPSAPPTLLAASQSPPLPSPPTPMSLPPPALPPPPALPPPSPPPPPSLQPPLSPPTPPSSPPPQPPLPPPLPPSLPLPPPPPPPPPPPPPPPSPPPPLSPPSPFPQPPFPPTPPSPPPPLPPLPPLQPLPPPPPPPPPPPPPPPSPPPPPSPPRPPPLLPPLQPPKSTLFSWPPPPNLPPSQLLPPQLPPPSMPSITPPPSPAPPPPSLPSPIPPTPPSLPPPPPPRSPQPPLPSPSPPPPPPPPPHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMD3</accession>
    <name>A0ABQ5SMD3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="424"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="476"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="505" mass="50345" checksum="BEF7DB84305B4686" modified="2025-10-08" version="1" fragment="single">MSAPHNDAVLDRVLTRIVSAGNQKSNGRSPRGISSVLQRDGVPARGNVGGDKRRSGSTTKFYANYRVTNPAFGSSSARDSSLAGTNTVVPQIVFAKGILRVPASSSAVQQSSATNAANTNGTVGAATATNAAAASMKPTSTVILRQPPPQRSTSSPQGHLRASRCSSPPLDISGAGTRRRSSPGDARASTGGGISGGGGGGGGAPYGGRSSSPRHAVSSCSGTVGSPLAVGASRSRSIPKERQSSGGGYGLRSSGGSGDGGMGLLAAATAGGLAAGANEAAVSGAAPLPARGRPGNTITRTAALRALKQQRSTTPDIIAELRAVSSLKASQQEGSTQAATQVIAFPAVGDAGHANPNAGGTATSTRQQQLQHQNQNRSRGEAAAQAGVASANADTAASSQSRNATPGGFISGDHQSSAGGGTSAGPARAAAGPGVAPRDTSPHPPAHPPVPQQQQQSLLNPPQKRAATQESQGHVQKQLKQQQQQQLLPPQPLQHGSVMQTQEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMR1</accession>
    <name>A0ABQ5SMR1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="558"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1413"/>
            <end position="1440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1549" mass="172745" checksum="345DA53B533821C0" modified="2025-10-08" version="1">MSRGNKRSQRQGAISGHPLRIATHNMRGLLASHGSRLQAAVRQWAEQRLDIVCLQETHHTTLQHQCLFERDLRYATKQIHTQNWKIAAYSWSNSTHTAGVLILIRQGLASALAPKLATPQHTCTRGRFTDCILHWGGHRLHICNIYLPPGTTSMAAETRRQLFENALAATTTQAMEDNRDTTLIWAGDFNFVENPEWDSSAGRDGRQADRPITEMRQRAGPQMVDTHRYLHPRRRDFTHLYHAPTPGGSRLDRIYINEAKLPYLCHTAISFHTASDHRLATLHLLPCKNEVKGPGIKRIRMAFTDHEDLATSMAEWIRNQCQEAPSVEENPGNLIAWWPGFKRRLTFLAHQLNKVAQQRRQQPGAHTQEAYLGVQQAQQALDTMPHSPHLLQQLIDAQARLARVHIEDNARVAKKTRVHWLHAGERPGPALTTFLQTQIQDQQTAPVALQAHSGRLVTNPAQLPQVVADFWAGVCRAPDFTTPEDRQSVLTQLAAANIRLPEEADEVAGSPTIAVTELGFALKKSQPGKAPGWDGIPTDLYRKFWPEIGHTMAALFTAIGITGQVPTGFLDGIIKVLYKKGDPKQPGNYRPITLLCSDYRLLAKVLANRLGPALGRIISPEQSAFLPKRLIGASVLFLRHLPHLLRKLNRQGLLAFLDFAKAYDTVDRNFLMGAMEAMGAGPQLCSWTRILLSNTRARAMVNGFKSRLVKMEAGVRQGCPLAPLLYLFVAQALFSWLKSQEIGLRLLPSDEDRVTACQFADDAEVALEGEESVPAFLEAMDTFARASGQRLNLDKMEILPIGAVDDHHQGSTINGLKVVTTATALNLPMDNQLQPPQPDWPEMLALAYTRMQRLAHYPLSAFGRAAAVSNYCLQTVTWHMEHGGPPPDTTLTYIERLAARLVDRKQGPEDQGQRFTGVPSRLLVGHPSTGGFGLLPLRQHIRARQAMWAVRFIRGALMARRERERVMLLDTAMNTVAEEEEEPVTSGNVRSLHPWIRTLATYLEITHPAFKPWALTTAQATGPWLGGETLPEEISRIVGALGHLPPLEDVSNPPLAPGDWCWAAALWGNPLLPNNSDNGGSINGNNHRPGLETRHPLMASCRKLLTVGDLVRVYDMLEHDQIPLPQYMTWLDWTQQFLDPSASPTQLRDKESTRMALSALCNDISPAWIAAARKVVNRLQPQQPDRVEDRMQQQMPAPPDRTTVMAMIVRRLGWRLPGDSGIVPLYNLTVSQATQLQGVSTAEERLELLSAFVQEAQGMPTDGPAPPCPVTNLQRFQATLDRLWTAVRWENKNKETLWRLVVDGIPIPGNTHLSRVPIKPCGCGSYGADTDQPCSPRLHHFWECPIAKAVREQIDRHLPLPTSATIEGGLTRQQLWLVQAPPGCEQAPWDVIALAALSSMEYGRHCLRAATREHRPQWPSTHRRRQQQQTHTAEQGPRQRAITDFFNRSGGFPQEEEEDANTHPIISTTQPAMDTPTQTATSPLDRAQARTVADFWARLHSFAALGIPHYGWDQVGPQHPILAIVGTQMRCADPVDITNHGAARTEQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNI0</accession>
    <name>A0ABQ5SNI0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045700">
        <property type="entry name" value="Rab3GAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21422">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21422:SF9">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="33276" checksum="9829B09D56BBC78A" modified="2025-10-08" version="1" fragment="single">TPQVSNNPWRRLWDSTAACPVWKQKPLQDPHLEGERVLHELETLSPVHLYDTLLAMALGAAVQLLDTSDGGSLLPVASLLRQYVRISAPVVKRGCSLVATSSAAAVASTSLSDRSSRAGACIGTPSQRPTGQLPSALALTGWEDAELEYLLAEFHYLEQAVVLGESLVRRLPGNRNLASDLMAATLYGNPFLEEGRTVMRPAAWVEGPMARQALSALLAGRPPPPATATASGRSPPPPPPLQRTTMAPPSKSHLHHQRTPQSQQSHETPKTQQSQLHHLHQRYDTDTPKQSHKDGSQEGGGVGRGPETGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP36</accession>
    <name>A0ABQ5SP36_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="8">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_016944</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF28">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="9">
        <location>
            <begin position="70"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="529" mass="58629" checksum="624850C920F7125C" modified="2025-10-08" version="1" fragment="single">FITFRVAIMLRIKGAKLALRALSARQGTELVQQRCFAAQPALSNEEEYSRFSQDTSRAHVTWPKVLNASLAEVDPDLYDIIEKEKNRQFKGLELIPSENFVSSSVMEAVGSVMTNKYSEGYPGARYYGGNEFIDMAERLCQERALKAFRLDPASWGVNVQSLSGSPANFQVYTALLQPHDRIMALDLPHGGHLSHGYQTDTKKISATSIYFEQMPYRLNEETGFIDYDTLEKTAVLFRPKLIVAGASAYTRHYDYPRMRAIADKVGAWLLADMAHISGLVAADLVPSPFGYADVVTTTTHKSLRGPRGAMIFYRRGVRRTDAKTGKPVMYDIEDKINFAVFPGLQGGPHNHTISGLACALKQAATPEFRAYQQQVMANSQALAKGMAKRGHKLVSGGTDNHIVLVDLRPKGVDGSRVERVLELAHIAANKNTVPGDTSALVPGGLRMGSPALTSRGFVEEDFDQVADYVDRAVNIAVGLKAQYPKLKEFREYLTKESPPELTTLKKDVETFAKQFPTIGFEKGTMRYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP59</accession>
    <name>A0ABQ5SP59_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="408"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="679"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="704"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="835"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1032"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="410"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="679"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="704"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="723"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="773"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1066"/>
            <end position="1075"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1081" mass="108490" checksum="35268F403FEDE9FD" modified="2025-10-08" version="1">MRYKRKRAAACSGSDGEDEILPSQHRKRAIDAMEGRLANLAGEVSALQSQLEDLRKLHGELAAEGDILQARLQGLPQEATLVNRPSDGHAPGSRSTRGSDPGSTFHFSMLTAAEPASPGGHGSAERQHPHAKTCRTAPDVRSADDQHDQLAHVHPLPVDFWDEIMMPNYHEEYPEAEVAASSEGLLQVAAGSTTGSPAAGVNPNGNASANTPAAASAISSVSRVPSLCAAMAVVPVTPGGGDGAAYLPSATPSNAVMAMGGIAAGTGLLPKLTQGGYPQAAAAGALGELPMGMTGSGGEAGPGPCAVPGVDSFWWHQQQHQLHQQQHQLHQQQLQLQQQLQPEFMRHYMEPLSYQELQPSPAHPGAQRTLYPHGTPTSAMGAAVTSLAVAAAATAAGSQVTSPLPLRFRQSQQQQQQQQQQLLQLPLQQQQQPQLSPHMLGDATPRRPGRWGTWASESCPADAAPLLGRGSTAAAAVSGDSAPAGDHKVSVSDALTAAAAVGSAADEIALSAAGPWLGASTGGGPTPPSLTHTSATPATPRTSMATAAVPIGTFTDSARDHSGGGGGVAAVSLLGGELNGNLSLASRFAGVAKGPLARGVKGYPAGGGPAAAAAALLHDAGMAPATGMGMGMSMGGGVVALGTSGPIVAAGNIGGGMSACAGVQCLKSAASAESVAALQEQQQQQPQPQRASERSQQHLLLQEQQIQQQQMQLQPPSHIKVESPSQSVGLYDSSGTGVSLRGNIDNTSAGASGLSHGRPPPLGGWPAGGGSSSGPAAAAAVSNPGGTDGAVTARDDVDGTACVGGAVAGQISIGPGRSEHVLTASQQQHLEIWPSGSEVAEKPSGSPCTTVQPSAPGGGGGGSLPDAKGSGSASEGVDDTGRMEGVAATSRSVQVGIRLGNPRCGDGLDGRAEHLPQTQSLDRMLSCSGGGAVSVAAAAAAATVLPLPGHGAPPYGHPWGSPPYGSPLPFTPLTYSSPYPVPIRYGASPVGRPVMWRRMPARPPSLVLPPIPAPGGMTTLLPPPGAAPGCAPLGYPMGSLPPQGVTSPLSGQPPPPQLPEAAADSGSGQVTSTISPPLRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPQ9</accession>
    <name>A0ABQ5SPQ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoxygenase</fullName>
            <ecNumber evidence="4">1.13.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_016671</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; oxylipin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.-" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.375.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001246">
        <property type="entry name" value="LipOase_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00468">
        <property type="entry name" value="PLTLPOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoxygenase" evidence="6">
        <location>
            <begin position="214"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="281"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1045" mass="115461" checksum="76556E5FAE55FFEF" modified="2025-10-08" version="1">MASVNSRTLNIADSLAGKRTKLLSASPAAGGLIRKRRSVSLRPHRTPLLQEKSSWNITTSAAAVEVGNIDTAPKASTGAGKGSSPEGVKIWKCSIRSTTPLLGDTGARLSLVVIDAHSELRSAVIDVDSWRPPKEITKSDGTHGWEREGAVSLPAAMKEPGAVLVRKQVENNRGASQDYIETITLVSTVSVHTVNANSWVSSDHNWRVFFSGFAYLPKDTPPTLADERRDELRAMQGTPETRVEERKETDRIYWFQTYNDLSSGKVDNKLVRPVLGNSKKLPYPRRLASNRGNEPDGREKAPRRGQQYWLQQDDEFSFDKKEDFQGNNIQGVLAGALSLVGEAASTTPSPITVLPATIWGLIRQVLGSILPTEAPLDNFESFKDVLALYKKAAGPNFRGLLPTNLYPAGSTASMDSDESMTERPSRFKTADGAWDFELSPEKAAEVDNFIAEQRVGPVYDSATGTPAKGTRSIFGVDSKPGASKTQERLGSKLWGVVKGQTPSVNNYFSFKVPRVIDGRPDAWHMDEEFGRQMVGGYNPMVITALKQLPESFGSAIRGEHVNADLQGSTLEELVSEAAAGGKPRLYYVDYWALSTFWETAAANNEVQKGKVQHAGRVVMYLRKDKDGNDTGLIPVAIELAHPDTHKEHPNLPHTAGLVYSRSKLSGDQGTLAVWRLAKMIFKSLDFSVHQLGSHWNRTHCVLEPFYISLRRQVSVMHPVYKLMAPHFRYTLQINRNARQQLINAGGIIERIVPSGKYAMLLASVLYGATWNFATEALPEDLKARGMVDPATGKPWLDYPYATDGLDIWSALTSYFDTYLRLYYKSDADVLEDPELQAWWNEAKTEGHPDLLKFGLRKDEKQLWGFTGPIPSVDKLGHVLATIAWLASGHHAAVNFGQYDYTSFLLNACSLTRRPIPAPGDAGWKELTSARPRLQERIILTYLSDPFTAVTVSSTVTLLSQHSRDEQTLDEVNELLVDPKARAANLAFMEEMKQLESRIEMRNTDVTSWARFGLTGDKGGPMPYTLLVPGSRAGLTMRGVPYSVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN06</accession>
    <name>A0ABQ5RN06_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="151" mass="16289" checksum="EA76F4E1F606C538" modified="2025-10-08" version="1">MKPGFGSKKNKKKKQKKQKSKQNGDAAMVDAGTMSSDDGIDGDVPNDASVATGLSAKERQKNKVELKRALKVKVAKLKQARVKLTKVPGNKVQRKEVSEAKQAALLELQELQKDKTQKKTIKKKNMNKKTSNAAGGKGQPSAEGVTAMDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN10</accession>
    <name>A0ABQ5RN10_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000710</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="208"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="388"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="465"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="261"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="581" mass="60400" checksum="11D9C2455A21D8AC" modified="2025-10-08" version="1">MVMNDTFIEPGQGKAGVATTTAQAKATKAQFLCLSSLKLTELPEGLDTLKSLTSLDLSNNHVASIPTKGLPDSLVELTITGCQLTELPTCIGSLPRLKKLFAGANKLQHIEPVFRSQSLQHVGLAYNRVAMLPAEQVLAASPLLSLDLSHNDLEGLVATLTQLSSLPSLAALSLGGNPLALAPRYVAETRGRLKQLLFLDGQCLDAPTSSRPGTANSMGASSSRHVQQQQSSGPAQGGGSGGGSGTALGPVPSSSRLLGPAGRRSFGGSAGAFTPHQTVRFEEGGGGAASDGTTVVLTLRGLRPAEDPFGWLKKRWDEQIATAEELGMTVPPTLEVPVPPLQPVIYHIEMTDVEGTPVACLPLRLVPPTPPDVAVMFDPKAKAAAAAAAAKEAKKGPKPATPSKGKKGGKGAPGNAEAAVQRQMETGLLRMTLPLRAITDHRNWLRLGTTVTMYKTILTAIARPPTAPDATEGAGGRSKPAGGAGGRKKKGDEPAPVEYDVTSSTEVVGSAILRAGAAVVDGYTLEATERLDFVPPPQLFDDKGIRMPLRDSRHPKEPVGHMDVHIRIHAPMPGTGAGGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNH2</accession>
    <name>A0ABQ5RNH2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Carbonic anhydrase</fullName>
            <ecNumber evidence="3 8">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_000947</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3 8"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03378">
        <property type="entry name" value="beta_CA_cladeC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015892">
        <property type="entry name" value="Carbonic_anhydrase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002:SF76">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00705">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="260" mass="28201" checksum="76484FB02846CAD8" modified="2025-10-08" version="1">MGCGISTEQHGDSTPITVVAPLSQAVSEANTLMLSEDKKYLSKVCSLSERSTCEQVLQSLLEGNARFIKGTRAHPHQDFSRVRQISEKQKPMVAILGCADSRVPTEIVFDQGFGDVFVCRIAGNIATSEEIASLEYAVLDLGVKVVMVLGHTKCGAVKAALSGQAFPGFIDTLVDHIDVAITRVDMLGVKAYQAVREGNTEVLDMVVQENVKYQIQRCQRSTIIQEAIQKHELLMVGAVYDLDSGIVNVIVTKGGNSFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNP8</accession>
    <name>A0ABQ5RNP8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000727</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI58939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="412" mass="44899" checksum="3DA32EE8470CC7CC" modified="2025-10-08" version="1">MTLRNVTQAVCQVSLFCARAAASSCAGCSTSSSGTSETLGRGLHRMFLTAAVPQGYIQTWADVRGRQVEPFSIVQQEVDIVSERLRHSVLTGIPALKTAAEYFFRHGMEGKRLRPTLALLMSSALSPSAPPPEYLQADLRPAADHAPELRRRQQRIAEIAELIHVASLLHDDVIDDAETRRGVVSLNNVMGNKTAILAGDFLLARASVSLAALRNPETVVLMSQVLEHLVSGEIMQMTATSEQLLDLDHYMAKTYCKTASLMANSSRSIAVLANVTPEVCDMAWSYGRHLGLAFQVVDDLLDLTGNSSVLGKPALNDLRSGLATAPVLFAAQEDPKLKPLILRRFKQEGDVALAMALIQRTQGLRRTEELAAHHAKMAAEMIRCLPAAQSDHAEIAREALVQITHKVLTRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP32</accession>
    <name>A0ABQ5RP32_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001018</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd21677">
        <property type="entry name" value="SMP_SYT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045050">
        <property type="entry name" value="Synaptotagmin_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774:SF190">
        <property type="entry name" value="C2 CALCIUM_LIPID-BINDING ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10774">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="297"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="596"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="509"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="761" mass="83139" checksum="C0646F6951F42875" modified="2025-10-08" version="1">MVLPFIGASIATGIGTLAGLSLGPYMYSNLIKPTSYRLLREEVDQDQKVPAPDPGLLVKTMYREALPLLEPWVTDPDYDRVSMINRLTATVWPTLTKAIMDLAIQGDVYNAVLYPQLKAQVFDKFAFVEDIFLGANSLRHGKVDIKNNPFLADKLFTVGSVAPRLGGMRVVPTADDEVLLETSLIWGSQVTFDVHVILRFGRLRLIVPLQLANISFKADVRVMIRPLVEKFPCLGGVSISLLRVPLVDFSLKLIKGVDIMALPFIPQIIHAALKVVLEPVTIPLLNKPLVPGLGLVLPNAMSFPIMPKFGLPDPPVGAIKVTIFNLEGVKGGDDMYCKLEVRKGRFKQTRTVYNTRSPVFNEDFALIVDSLENDALRIYVYEDDVGWNDTLLSEVVLPFGEVMGTKVDPATGKEVTDFQKADFLDAEGAKTFTLQLRKVHKKGALKSASSRAASYKSFAATKASRSPSPFPAAAAAGDGGPASNKTSIFNRIFNRKKAKKAAPADAAAAGAISSGATSDAEGGPTAGPSSMMSMQSTLLSGRSNTGNNAEDKDVIGTITFSVEFIRFNKPEYDDVNAEKEEKSKTRDPYLSMFPGPPGRTLALLDGSSGGTSSKLSYGVLTVQLIRCIDLAPEGAKDLATYVRMICTNDEKDEVQTSSTILNETSPRWGDKFDFVLIPCASELHIHVHNKPGVLGNLMGSLLRKKTEDKDPILGKVLINVEHVARNGRIKEVWSLEDAERGYIELNLTWQTCHLSKDQQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPF1</accession>
    <name>A0ABQ5RPF1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UVR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="37847" checksum="8C1D2024067BB507" modified="2025-10-08" version="1">MLARRSCSSISYSKRTSTAFGCSIWHQRTYHIHDNAHYLRKSSRYDEVPASRGLEVVDVDVDETWQRFNLIQRKLKIAVSDEDYVMAAKLRDEQKTLLEGLSPVQQYALGQVARLRSGTVQEQLQTIRNLAEAGEVTVIPDLATCLAVPELAGAAEKAMWAIFMRARRTTSGPQCITSRAAMRTASRTANWSFRCSHFTSVRHQAWVCACSSCPVSPRHWRLSKRLLPSTREWPISSGSLRTCGATFRPMGMGRGCRRWWWWQWWRQGFGGWEADEGPRNGINPKRRRRRGMGKPMHTSRHPNSRTLSMFHLTEAVERWYWKAAGFEIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPL4</accession>
    <name>A0ABQ5RPL4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001326</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the citrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06115">
        <property type="entry name" value="AthCS_per_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF4">
        <property type="entry name" value="CITRATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="536" mass="58506" checksum="50926EE73B61DB28" modified="2025-10-08" version="1" fragment="single">NINWRSIAFPGGVPLEGLSYFLLQSELSGMSSANQAAGRLAVLSRQLTGAPADETVARNDTSAEEEQRPAPGGGRGTLTVVDNRTGKKYTLEISEGGTLNAQALKQIKAGGDGVGLRTYDPGYTNTSAVISRISFIDGDKGILRYRGYPIEELASRSNMMEVAYLVLYGNLPTQSQLATFREAVNRHSALPTEVEDVIAAMPHDAHPMGVLMTGICALSTLHPEQNPALAGGNIYKSREMQDKQIVRLLGKVPTMAALAYHKSTGRKAAPPNQRLDYAENFLYMLDGGYTPSYRPNPKLARALDIMFILHAEHEMNCSTAAVRHLASSGVDVYTAVAGAVGALYGPLHGGANEAVLRMLARIGSVEAIPAFIAGVKSKKEKLFGFGHRVYRNFDPRAVIIKEVAEKEVFPLVGVDPLIEVAKALQDAALSDEYFVSRKLYPNVDFYSGLVYRALGFPPQFFTVLFAIPRIVGYCAHWRESLVDPDTKIIRPQQDYKGVWLRHYKDMGGRTAEGSDTLAKLPPSNAYNRRVAGENWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPT1</accession>
    <name>A0ABQ5RPT1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tubulin tyrosine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001421</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051437">
        <property type="entry name" value="TTLL_monoglycylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870:SF2">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="210"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="483"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="609"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="680"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="811"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1183"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1259"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1366"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1426"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="224"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="300"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="515"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="549"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="693"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="707"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="729"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="811"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1262"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1319"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1366"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1429"/>
            <end position="1442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1573" mass="166497" checksum="1C0DA9F51F57DDAA" modified="2025-10-08" version="1">MLKEFNDAKDTDIKLSSWEARNKKYIQQLTMQKKQAQLQSQQAAEQEQKRKLRLSRRVLGASFQRLQSQQAASISRSLEAGSNSASDSDVGCGAWPGPGVEDGSNPKLERTCSHSQIPKLPRLADAFEAAGGVTADEQHRCSGTSPSQAADVSSFGGIVSGCGGGANAAGQPYVQHSLQLRVGGGSLRAPNQLSRSVSAQRTVAAHLSSCYGENGGSGSGGPSYTRNPQHSQRNTQNQDQHLRNRQRRSTLPDPPMKPVRMAANGCNNDPKDSNGCNDDDDGPDIALSDRRRARRERPFRSQPPTQPQAPALAVGARTFVRLAAAASFPPTPGGYVQWRARTRLPDGQRTFCIAGTGNHSHGIRAGLLARGWYENPDRNSPFFDFKWAANPLDINHDTLWPGQIVNHFRHSTCLTTKHGLCYTVRDTVWYEAADAAAFYPRAYDITDETSRREFMHDVRWTAAESLIKRAISDGAVCTTHVPYSPRRKSINHGPQQQHGKTSPVAVAAADAGNGHSSGGDGGGGGGGGGSKGSDVTPLGDGSGDSTAYNGGGCGGDDSGGGGGGEAMHRLQQRADSMPLVDLPSLRLACRVVAARMRHQRHLRVVHESGTGRVGGRSGSCGREGREDTDEPLPDVEFSTAQWQQLVKHRALSCSIPVLPQPQFDPSWIQNRSFITSALLPPQSSVPEEPKRSCGGGGGGGGGGRGSPVRKSRASRKGKDPQRHHRRRGGGGGAGTGTDGETGCGSDGEDTDGSNAGGDDGRPSVSASFAATGSAEDVDTDGDARSTVAAARPSGPIAVVDAAELLRKVYGKARRGHSAPPSHRRQGGPSKFSAAAATGAQSEPDMVDDLGASMSVPVHTSSSPQWEASLRDVSSGDRDAGGEGSSGGDSDDACASIGPSWLPEDLPAICYDDAALQALDLDTWREAAVAILDALEGSAVQTHISGTNNIWIVKPAGKSRGRGIRLFNDPDALLSYVRGEEAQGLESRWIAQKYVERPLIISRRKFDIRQWVLVTGWNPLQAWFYSTCYLRFAADDYDPNNLDVFQHLTNNSVSKYYEGPKKEDEITAAGNMWSIPRFQQWLAEVYGRDDIWQVLLQPAMRHIAICTLKSAQDHITPRKGSCQLYGYDFLIDDQLRVWLLEVNSSPTLEPSTPITAQLCADVQDDIIKVVVDLPEQQSAAAKRQARGAGDCGEQASCDPGISRGGTGSLEGFDTGKWECIIDGGEELRQPQYTGLNLELKGTAIQLTPPRSNFAGRLYAATRQQQQQHGSAGQHQHQPTSAGANDISASSGGGGGGDREVTTDDGATAITNGRPSRRSVAGAAMGIIPAPPSRAATPSPTQNPGGGALDQHERQQLILQQQQPYFHLQHQQQLSMQQQQQRQQPNPHHMQFAMGTGEEDAAATAAAAVTAAATSSSAAMLARVYTGTCTPSSSRGGRASPARSRTPRLSASPALSTHWDGGGFVLGSFGDPAGAISMLACGGTAASAASASGGAGSPLHPSSRIMTPRSDVAIATLSVATTQAVAAAAVTPVWLPMSLPQSLDSLTPTTVRMSLLARPGHHDNTGLTPAGVRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPU0</accession>
    <name>A0ABQ5RPU0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="121" mass="13271" checksum="D15B27D0027D4D43" modified="2025-10-08" version="1" fragment="single">NGCNVCNMRRSEYRYSVADPTIAIVAFPHTRMVYDTDECGLERLRPVHTLYSLRRGGASAARAAGVPLDIIKAFGGWATGSSALREHYLDLGVRGCPYAFGFFSPLAARRVAPVAGQFFNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQG4</accession>
    <name>A0ABQ5RQG4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="9">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_001743</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="9"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14386">
        <property type="entry name" value="UBA2_UBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF664">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="13">
        <location>
            <begin position="170"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="12">
        <location>
            <begin position="326"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="11">
        <location>
            <begin position="740"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="11">
        <location>
            <begin position="815"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="701"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="784"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="715"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="798"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI59780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="966" mass="102293" checksum="22B90566C6694A9E" modified="2025-10-08" version="1">MSSKAPVDEAALVRVRQQMHAVRVPLYGDKVYKDECMFTYDSPESPGGLYVNLTTFQGFGQEMVELDQRRTGGVLYLHLRHKRVPLPKSEVEAARAAAQAAGQMLLNAPTYRMDKQQCLVVMGQGGAGRLEIPLPCSDLPERVLQSIEGVLRHESATAAEAVAVFVEARPVSKYAASLEQLYTCRKISPNPADWRCDETGVRENLWLNLSTGFIGSGRKNWDGSGGNGAALRHFESTGRRYPLVVKLGTITPHSADVYSYAPEEDDMVTDPHLAQHLAHWGIDIMQMEKTEKTMSELQADLNTSYEFSRITESGAELQPLSGPRLTGLINLGNSCYMNSVLQVLFTLPEVVARYVNGAARIISSAPPDPAGDLPTQLAKVGVALVLGRTGHLKDQVQQDGEAAETSGGGKEAAIEVDGGGGDLVALPEDERWHAVRPLALKSVAGRGHPEFSSGRQQDAAEYFTHLMELVSRAEHTQAERLGLSPAAGPGAGDVAPGPLPLPTCFRLSLEDRLQCSESGAVSYRSTPTSLLMLQVPLEAAVNAEAVAEYRDRQAKRQRLKEQQAQVYIGAAPEGADASTAVGPDGSGAVASAACAPHASGPGSGGLVVMADAAEEAVLPRVPFDACLQRFSGTEVVDDYRSAALGGRRTAATKRTRFGTFPPYLLVQINRYFQDTDWTLKKMEVLVDVPDHINLETLRAAGPQPDERLQPEDQPEQQQTSQPQTAASGGAGGPQQAAVVAPDEALVSALVDMGFGANACRRAAVAVANGGAEAAMEWLLSHLEDPDIDDPLPEPTHAGTGGGGGGGGGSAPAAVAADPDKISQLTAMGFSETQATAALHACNGSLERAADWLFNHMDDLDTAVTRVLSTGDSGAAGGGAAAAAVPAAGGLAAGSSLTPQPQLLDGPGVYELVGFISHMGSNLACGHYVAHIKKDGRWAIFNDDKVAVSEHPPRDLGYLYLFKRVHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQH3</accession>
    <name>A0ABQ5RQH3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxin-14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="392" mass="41415" checksum="E4E16345EAE00C19" modified="2025-10-08" version="1">MVAVQPAGPTAQVPRVVQRYRWSQVVLGVGAVFTAGWAVHQLVVPHLWALVESWQTQRREAEEARHKELCAAVASLKGCQEELKTSTQALLDALTIMREQQQRSSTIDGIIPTRGAAGRDNYYQSINGRAEIPYGGSGGNTSQMLGADNYAVLRAAAGYKNAYPPDPPSYTVSYNYGRDSGPATAGPTGGDGNGRNGGAMYGMSNTPYGAVRSVPANSYGGPGSGGSGQQQPQQENLPYRVTAPPSAHSTHSTQIGRQQQPAGGLGSPSGPYGSSDRLYEQAVPGGARQGTKITGVAGTNSNAASGGGAPASADRRTVPPPPPEQPAYPESFLSLMEMVQQGITPPNVRTDINDAPPDRASQAMGVSGRGGWKRQWQQQWQRRQWACSAWQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQZ5</accession>
    <name>A0ABQ5RQZ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002045</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592:SF15">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="3">
        <location>
            <begin position="290"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="404" mass="42646" checksum="8920B2AD2097C54B" modified="2025-10-08" version="1">MRDVGLRGNRAAAGWTIVGTSGSILVDWPPRRFLVSPSLLPRVARILCFAAGPKAQQQRDSGPLLTQPAKLWGKADSKKPQVSAPSRPSAQPPEGNGDGPDEDSTWGVLDVSRYSGSWQVPWGPGRVAGGIALWFGSFVGVGFVLVPQLYRMAGISLSPMDLSAEDKATFTLVCQAAETVVSLALIRALTAGPIAEASSTSHSSSAPSTISTSTSRPSEKKLDFFVYDLRRPFSRPNGWAFWALLGVVASPVVVGSVATLLSVVGYEQVVSGKGTVDGVAGMVDLDLPTYLALLAVTGVLAPVLEETVFRGFLLTSLTRFMPTWAAVVASSVSFGLAHLSARDLPVLSALGLLLGWAYVRSRNLLTPIVIHGAWNSAVLSLLFWLAAEGVDVQQLISDLHDAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR72</accession>
    <name>A0ABQ5RR72_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SURP motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_002084</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI60020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045146">
        <property type="entry name" value="SF3A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022030">
        <property type="entry name" value="SF3A1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316">
        <property type="entry name" value="SPLICEOSOME ASSOCIATED PROTEIN 114/SWAP SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316:SF1">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12230">
        <property type="entry name" value="PRP21_like_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SURP motif" evidence="9">
        <location>
            <begin position="68"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="9">
        <location>
            <begin position="181"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="566"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="245"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="322"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="570"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="611"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="647" mass="69307" checksum="F1C515DA1D886030" modified="2025-10-08" version="1" fragment="single">MENETDNAGAIVPAGEARTVQPSTDIVIAGDAGIGASAYIPDIEGLKKVITHTRAVGVILPPPDIRAIIDKTAQFVSKNGVEFEKRILSNEKNNVKFNFLVPTDPYHAYYKLRIKDFSAEDGQAPKDQAPAGVVPAAPIAPVPVMPISTVPKATVKPLEKPDEELYTVHVPEGLTTQDLDIIKLTAQFVARNGKSFLAGLSSREHTNPQFNFLKPTHSLFAFFTALADAYSRVMMPEKGLKERLAKDAADRAAVLERALRRLEWERHKEKEAQDAADEAERERQAVQAIDWHDFVVVETITFDEEEDPELPPPLTLRDVIAMNKSREALEALNLQQQEAAAAEAVKEADMEMDEEERAMVAEAAAQEARAAAAAAGNIGNAPGEGPEGAGPAAANAAPEPASEDADAAIDMEADMEEDEEAPIKVVKNYTRQVARTQAGRSYDPAKFVVSPITGELIPTEEMAEHMRISLIDPRWKEQRDAMLSKIRETTKATDDEITRNLVGLAVSRPDIFGSTEEELQQAVREEIKDKMMSGVGRPVAWDGATQGGEALQNQIRAIADSRADTAERNAAAGASAGSSSIPQAVLLGPSARPGGGSGPPPPPPMGGQPGGMMQRPPMMGGPMGGMMGGPMGGPMGMMRPPMGMMGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTE5</accession>
    <name>A0ABQ5RTE5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014954">
        <property type="entry name" value="DUF1825"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08855">
        <property type="entry name" value="DUF1825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="260" mass="30356" checksum="3FD0E372218D5E99" modified="2025-10-08" version="1">MASALVFQPAQFSLMIRRGKEHPSCSYVQLRRFSQNSRVTFQQRTRHVIRSTPDEDYYAKLFNSPIFRSELVLQEFNKMVQETVSLSQLAAKFPDFDLTGKRVYLDKMDEVSSRYEIFIKRLELSQDPVAREFLRFTTAQMLEGGLTYSQMFMGLKQSVEQYRQWVEQEERVSNNPVAHQQFLQGFRAMWEASFLGRVDLTYLFEKVDPGVLMKAQADPRYWVAVKEIATDPANMGKWLDDPNLGPFVAALWKSMQDKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTM7</accession>
    <name>A0ABQ5RTM7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002733</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044237">
        <property type="entry name" value="ATXR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47436">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ATXR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47436:SF1">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="28"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="601" mass="63492" checksum="65F81FFF7B842616" modified="2025-10-08" version="1">MSLLLLQSYDGESQLSYYNQVLLNANASSFEVILDVNGKGKGLVATRDITEGTTVFIERPLVHLQHLDNRGSAIVCQACLRFVGSIERQVGHKLRCLVDQLRGAQRGTSLSDGDGGDESEIDLDGALEAGERLEQIYSHVTPEGIAELCSGTAKLPLSDGFQLPDPVPCRRGCVALYCSAACEAEAWEHSHCLLCPVSTSTGPAAAAAPAGAYSPGSAQAAVTHSGGHSDTAEAVSSPDAATNSTGQPTSYDSARADGTAGGSAEAAAGGGPASAGPGPSCFSGGPTAASPSVTAPTEAVMEELCGISIDRRALVSFWEHARETNEIFLLAAQVVAVTLLKAARLVQVDELSEGAPVATTPGPSSQAAGSSSNACQSALLAAWRPFMYGWKRCWWEAVAVPDDVDDEEEFRAQLRSLASDSLELLSAAIKDPRFGPDLFQLQVYGSIVGMFELNNMGLIVPSPVEDYFLEVDEMEEGTEKREVTQVTQPLLDALDAEYATPCEATAFLALQSCINHSCDPNCTAACDTGDRTVTILAQRDIPAGEEITVSYIDLSLPYKERQADLRDYGFVCRCVRCVAEAAEARAKRKGTAKGVKVGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTV1</accession>
    <name>A0ABQ5RTV1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_003301</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045150">
        <property type="entry name" value="CYB561D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422:SF45">
        <property type="entry name" value="CYTOCHROME B561 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422">
        <property type="entry name" value="OS05G0565100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="12">
        <location>
            <begin position="58"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI61037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="204" mass="22045" checksum="3E83770DC47A490E" modified="2025-10-08" version="1">MLLPLLGIAYAGVVLYNVLVVSKLSLNLLYTWHPISANLFIVFATLGMGAAQGIRGKVALSRATKEPYVTRHAVFNWLAVFFLAGAGATIYLNKERNNRPHFTTYHGISGGLTGAFFVANVLGGGAMNTVPSLYKYIKFHRLNGYLIFAAVLAAHGTAVWRGYAGFKTDEYGKYALVGLLGLMLLVTLRSIKLSLLPGSKSKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU86</accession>
    <name>A0ABQ5RU86_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_003381</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF10">
        <property type="entry name" value="SLOWPOKE 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="15">
        <location>
            <begin position="170"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="16">
        <location>
            <begin position="262"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium-activated potassium channel BK alpha subunit" evidence="14">
        <location>
            <begin position="451"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="661"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="848"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="993"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1056"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1409"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1463"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1560"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="666"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="861"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="999"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1409"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1434"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1491"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1560"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1584"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1631"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI61100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1658" mass="179434" checksum="ACBF653A3A95FC51" modified="2025-10-08" version="1" fragment="single">MWSKMLAFFSAGKEDASRISSRQWFQYKFNKSMLRVALEVINTFMSVLSVSVYVYDTYVSIDDVARIIYLTCSCTFSVEYVAKLLAANDWLSYMFWDWSLIDVATIAYGIAYYVNNSVSNDLGFIRLLRIIQVVRLCKSITFIMLQYSRHTSQPMQLEFVRRSADLAVTLVAVVFISGCVFYELEQVQEGLSLHTAIYWACVTISTIGYGDYAPETVAGHLMFPLVIIIVILILPRKISDLFEVMHSFSRFVRRSHRSHHFGQHVLLTGHVTSQSAQTFIGEFYHQGRGYQDMDVVLLCPNDPEPSLVIALNNPRWEHRVKYLNGSPYQAEDLARADTGRALAVFVLADKYAADPAAEDNRTLLAVLAVAQFLRQFGSPEDLQPPRVVNWSATSWKGRYNCSDASSCSHPNIVAQFLLSETLNHAIALLRSGARHPPELGLIPLDVSPFPICVGSLRASLLGAALHTPGLIAFISNLCISASLKRRSWLGHREDVSMLTNCGWLSEYFRGASVEIYDVTFPESFWGLTFKQAALKLWREEEVILIGLHPTPGDKMELSLEVKLAPMGSWAARHVASVTAADLQQSRAADAAADEVGQLPQLQPRFFHSQSRSKLQPPAQQEEVLVDGHTVGYILSADALVLGSGSRRPLAAEEKVEAMWRSTTGMQHQHQQQQQAEPQHQRRREEQTHQSPPQLASPLITPELANQRLQRAQQWLPGAPVKHTASGTMAAGTTAISSPRAVTRLRCDKATIAPSGTTTTGEMACAKTDATTAGHKTAAISATTLTMAAATVAAGAGAAAVVEDTVGNHGAILERIPAAAPSFCSGTSVECGLWSWSAATCSHNPSLLQLTASAPPPPSPSPQQQQNSVDGTPSPSSAASRRGAIGSLPPATQAAGLHTGTVAPSPAVAVSLPAVASSTTARARPFWWQLASMPREHSQHTEPCSDTQQSGLVKLTVEDWKPNRVTETNPPARQAKWIWRPSFRSEGRLGHITASKRDVSGAGSASGGFTSTDASTTGGGASGGASTKRQQGLKATTRAPDLHALAAVSSATYKRATEQHMSKTVMDAVQGRPSDSDSDLDLSFAPPSGARPVQGPSCLQPRILDTFADREALDSPVLILGSSCFIQLAQLVGAIRSAPPVGYGESFRTRTMVIIADSAEDGKAALGSKQLAALGSLPGALPDHIFVLRADPRDATDLEKLNLKPGCSVAAVLVPSTEQFAKGGVKGAAPALADGQLLVAAAALRRYCDQLGCRLRVVAEVLALLNCSYALPLCLEEGPYHTNNLTGTQQLHSYRRPASSLIESPDPLRSPCNHVKFVTDPRPDFRRVVRMTLKRLRRFPDEDLQEEFVQEMLSHKPNLTPSLAPGHILMETFMDALACQSIFNPMILQILLKMLNCWTATPTHHRRARLQNHHPHHHSHQQQQQQPFQHEHQKQARRFHLRQQSHPHVTPRYSRYLQLRHLQHPQVPQRPPLVSPSQRRKEHPDRSITRGVPVVASSSPAPSSSPALFLEGRPKHTQMPLVASPHPGAVAASSDYVSGSILNPVLATGCQSISISLAAPAGPAGGTAPRPCPARLTTSESPQTQSPPPPSAAAAPTPPVEPVPVKVPSSLQQASHNEATVAVKASTRGPARFSRSDGSGIGISRQSAIAAASAATTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV22</accession>
    <name>A0ABQ5RV22_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47467">
        <property type="entry name" value="OS01G0867200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47467:SF1">
        <property type="entry name" value="WD40 REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="465" mass="48130" checksum="64F4947DFA4C88E8" modified="2025-10-08" version="1" fragment="single">MADPTATLGQRPSKRVKSGSQDKGPDLQPCALLKYNLKLPQSFTPGSLQANRVALRVAGDSCLAYIANAQHVYEVSLGSDLLAARSSSSGDVDQGKEGVLVPRNAQVGHIRQMEPLSNMAAEVQGLYAGIHGGYHVLAAVDSVGNARLLATAMSAPPSGDEGSPGEHQLMALDVPCRGECGWTGLAVRSVGSVADGGGAEAGGALFEGPLLEVAVARQRMRDVLIYRNGARLRTLHTMQGPTALAYLGSAGSEAPGGLLAIAEEHQISLWDVQQGGRGGCVQRLGVYGGGCPVYGLTWISTVAAAEGRSRQQGGDSGDEGLLAATGGARSVVMLEPRKWHIVAKWPGCVKYPATHMRPSALAPGYVFVAGLDYECVAGRWDGRGGSCSGNANSSSGGHGRAMGPAQLIPRLEKQQQQADDGLEGATCGRVGLSFRGDSKWLGLACCSLPPLPEAVGSNRDGGCRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVH9</accession>
    <name>A0ABQ5RVH9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SOUL heme-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="entry name" value="Regulatory factor, effector binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_heme-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="HEME-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220:SF58">
        <property type="entry name" value="SOUL HEME-BINDING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="Probable bacterial effector-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="24219" checksum="60844DFC1F981865" modified="2025-10-08" version="1">MATVFGNISVETPKFTVVQSLGSSNAVEVRKYGPQVRAEVLYDVSPNHSVMDGTGTPFRALAGYIFGGNTARTRGGSEKVDMTAPVVVQTTPPGGASSEKIAMTAPVVMQAGTGAGAAADGNSDGGNKHVMNFIMPSKYKSIADLPVPKDSRVRLFEVPERTFAVIRFNGRMNAALAKTKEQELRTAAAEEGVKLSDNPHAVQYCSYNPPWCLPWLATNDIMIPVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVS4</accession>
    <name>A0ABQ5RVS4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="16615" checksum="0C40FABB44F37BE4" modified="2025-10-08" version="1" fragment="single">GPPVYPDTSYRRLMVDSLGLGLPTDDDMIAQLLNDIEGGGPGVIGEELFVRVEPTSSSSSADHFDILHACGATEDTTITALETSSNKNQQGYSNELRQQNGENQEREQSFNELVELLRSDIAHAGFIEVLTWPLVLHEDNFRKLRRIDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWR2</accession>
    <name>A0ABQ5RWR2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein MON2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004631</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="3">
        <location>
            <begin position="6"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2 C-terminal" evidence="2">
        <location>
            <begin position="1604"/>
            <end position="1765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1091"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1192"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1242"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1408"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1458"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1531"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1955"/>
            <end position="2044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2398"/>
            <end position="2440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2627"/>
            <end position="2659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1118"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1195"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1242"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1417"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1557"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1955"/>
            <end position="1989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1994"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2011"/>
            <end position="2036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2645"/>
            <end position="2655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2752" mass="279237" checksum="6E593D8D4BF228C1" modified="2025-10-08" version="1">MAADVVLTVVETDLRLLAAEAKKAEGPMTNLMGMLHHSDLPALKDATERALQKVRTVEQSGLAGVQQALEILAPFALACETKYAKLARIAISAFQKLLANDAVSIKGRCEIIKALQAAERLSDESVKLRILQASLTIIQSPSFADEQDAIQQLLSLVFRLYINNKSNFAVHSTAAATIRQAVALVFDHSALPGRVPGATALLTGSPAVAASPRPSLATPSRSGGSEGGANSPGPPQPGGVGGGASAAQPVSATGAVAGTALGADNGAAPGREVVPVLLLEEIMGWLGAREGGKELGWVQLQQRGGNRIDRTFLLEMLEAVLLQRWVAIHRLPGLVQCMKAKVTPAIHGLLDSACDRLVDPNDAVDCRLVVRCSAALIRRHHSLVPERAAAMVVRLAETAGSVGRSASAHRWQRFMALQALRSLLADPSLLYRLYHLQLPADKQPSGISPAAASTAGKVVDANGSGAASTASDAGPGGPSGGVVHLVVQSLHEALRWYVRTSIETPEDDVVAALGNLYFQRAMGARDAVQETEAFGTNLAHGSEVVIAYLALEGVLAMVAATEALTDVVVVPAEPGMPSPKLITRDTPEVRCAAVAGLVLELWRCVLNVCNTLLARAGHDVLVGLLVRGMQGVTYSAGALGVMEARDALLHALCAHTLLPPGEDDVAVLAVSMPGVNIGAAGALAAEDRSVGGTSRRSLAITPAVGSSASSTLAGDGGACPAPGFLTALLVERCASSVRVVLTPRNIAALRALFNIAHRLADGLGSCGWLYVVDAVNALDRILASPHTTTAAYLVETGGEGEIITGLQPMNSSVMPTSGATTAVGAGAGPVGTAGAAAAAAAGVGAAASTSGAASAVIPDGVADDGSTTAQRELRILASAADQLFENTHHMSPEAVVSLLSALADISHRTLTGLGLLGGASSASGVAATAAAAAAQPPLPSGSSGAAAVAAASAAVATVASTGAAGGGVLSPSAGGPAAAVVVSGPVRLCALNRMVDTLLHNLWRVQDLWGIFLAHVLEALASNNVQVRAAAVDALDRTITGALNPDLHVPDKFTAPLPALEDALSPAAGVAARKGVLPTFPSYATMAIPSAAGGTSTPGSRRSSKDLPFGLGAAAGVTTPTSSPQVSLHSSIGRGISGGGAGTCAAASIPLVTSTAAASPTFLSPAPLRHASSGGGATPAFAMGRVSADLSSHLPPLPTGAAPPHPSSLASASSPAAAISSPFALSLTTAAVAAVNTSSAATAATATGTPGNTAATASPTPGTAPPVANRGVQQSAQREDVQHMLLVALDSLYREGGRSPDVRHGLLRIALHVLQHHGDGLTRGWVPLLRLLDGVPRAGQGSADVRLGFQVVELLATDYLASSLPKEHVAKALAVIAKFATQDTVLNVSLSSITMLWNVTDHLARSRGTFSKAAATQHQQGQQQHHQQQQHHQQQQKHLQPHLAATMTVTAVAAASAPSMAARAGPPPAPAPHSQLDSSSTRPFGGGSSGTAAVGSSPFSSLVYGSDTSGNGERQMSPAFAAALSFARGVPSLPSASSPERSPWPQPQQPHVKQESQLQPGQQLAQAGGRSAAIVAGGVSASGSGDGIYPLHDLDEAESIAMLMIAFRSLKALSVDPRPEARNAAVRTLFLAVGSHGGKFPPATWHELFWSLLFDLLLTIHRVSSTSSVEEAAAVELGKEKGGRSVVMLVHHSRNSEQKQWDETLVLALGGAAKVMRSYMAVLSGLDVWTTAWEQLMQLLGELLATGRKAVCLASTSLLTTVLQAHGSGPLVSRTMWQQTLAALDRGVNAMAAPNTSAPLQARAELLTCITTLSSQLLLSRGTRSGSSATATGATVVLASTVVTDAGGDAAIATVATNPEPDPEQLLLILGWLDRFARYPVGTDDTSSIHFTTIGPIQKATLAAMAALPPAVTAAGVWPAVLAVLCNMLRPFSTLALRQYAQQMAALVQLRKANSPAPWSSSSTPEPSSHPSTSGTPTTTAGAAPGAGALDSMASCTSAGSSREPSVHNQAVGSSSPVAPSAAGAGAAGSGVSPAGNSGGSGSQSLAFAASREVMEARALSPLWMARVVELLAAWYRDQVSWQVRVATFPLLVGAMSECMACRHLLPQAQPPSVAQPPQGSPLQLVLGGDLGEVQALEELWRGAARGFVTVVQTGLPSINICSHQGQHHHPHHQQQQQQQVMVPHSTWPALARAFQMFFLAANLPPLQQPPQEAQHSLAVALAAAGGSSATAACFRRLSSEPFAAVAGGAVLPPPSPEQMAADSEITMAVLDCLSETVLGSCHHAPVEMRRALMEVLDAGAASAAAPSAAGIHCSDRPYDESSLPPTSRLSHVCLNKLYVLCSRGQDTPVTLSEQDGHDGVQQPVASSRSQFEVSQLALPVFVTRCEAILQEYARADQRVKPASTSAPPGTPGGCTASSAPADSGAPGGGAIDGSATAAAQMQSPLRPGLAAGIVPMTLHDKARHVLELLQHLRVVPPVSDSLLPARPHLRFWLDVMRAVRSHAIMPPPSLAAGAAAAAAAAGSTTPGLALPSSSSLGGSGGIQGGLMLTPLEVSRRSSCGVELASPTAAAAATVAGSGGGLGGWTAAESLASSGAPAGRSVTSSFFSRTYSSTGALLSSALGVATGGSTTASATPPTPPKRPSGSGGGGGGGSGTTALQPLEARTSALAPPRHLATACMTSGAAGSAAGLQLTGKSLWVKEQPQLPALYSVLCECIGSSDPIVRGVVQGMLAALGGGLGMCAAPPSSSTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWW3</accession>
    <name>A0ABQ5RWW3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004486</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01283">
        <property type="entry name" value="cytidine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013171">
        <property type="entry name" value="Cyd/dCyd_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050202">
        <property type="entry name" value="Cyt/Deoxycyt_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006537">
        <property type="entry name" value="PRK09027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644:SF2">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08211">
        <property type="entry name" value="dCMP_cyt_deam_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006334">
        <property type="entry name" value="Cdd_plus_pseudo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="3">
        <location>
            <begin position="72"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="3">
        <location>
            <begin position="228"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="339" mass="36831" checksum="91ADBD33685C5749" modified="2025-10-08" version="1">MQSMHRQVHLRTSKLRKIIVGRRPASTGAASVATEQSYAVPPKIKEDNSPLKGRFMIPAVEVRQLLDARGNNFEELLFSLITPASKLARPPISGYFVGAVALGGSGNVYVGVNIEFPGTPLNNSIHAEQCLLVNCLHSGETSMQALAVSAAPCGHCRQFYAEIKDADALEYIFGRDAKPKFLADLLPERFGPSDLQDEPFPLLLEPQRNPVTWNLDAEAMVAIRADDQEFQRAATEALVEARQCYAPYTSCYSGAALITTSGQVYSGGYIESAAYNPSLSPFHSAVVDAVTHFGLPTYDQVREVVLAERPGALVQHVDNIRLLLSRIAPAAKLTVLPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX64</accession>
    <name>A0ABQ5RX64_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5'-nucleotidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004558</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008380">
        <property type="entry name" value="HAD-SF_hydro_IG_5-nucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02244">
        <property type="entry name" value="HAD-IG-Ncltidse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF22">
        <property type="entry name" value="HAD-SUPERFAMILY HYDROLASE, SUBFAMILY IG, 5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05761">
        <property type="entry name" value="5_nucleotid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="645"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="645"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="680" mass="76860" checksum="D935985F36A7F493" modified="2025-10-08" version="1">MRLRPLGAWAASNTLALRAHRVAKVIAHVRGRAKTLRNWDKLWICQSGPGGVSDVLQPTSQPVVDDPAVATEETFATSTGVVEPVQEGAAEIDFLGESTEGDLHFHLVDSLRKGKVGVINVFGMKSLDDIYSLPLARLKAATQSVLDVLDVPESFPDGNPQRAIYCSRTLNLRSIKAIGYDMDYTLIHYDVNAWEGRAYEYGLETLRQQGVPVDGLRFDPDLVIRGLIMDKEHGNLIKVDRFGLVKRAMHGTRMMSWQEIRELYGREVVNLRNEGRWVFLNTLFSVSEAVMYMQLVDRLDLGMFQVGAGNISYQALYGMVSKALYRTHVEGKLKAEIIQAPERYVELDPEMAQALLDQRDSGKQLLLITNSDYEYTNKMMSFAYDPFLPAGMRWRDLFDMVIVMARKPDFFNYNMSLYEVVTPDGLMRPVLGACKGGLYCGGSARMVEKALGVEGDDLLYVGDHIYTDAALAKINFRWRTALVIRELELEIDALARGRPHRDALKELMTKKELIGDVFNQLRLSRQRWVHGHIAAASFDDEEGINETLAQLLMVMEHLDDRIGPMLERDGEHFNKRWGYLSRAGLNDKSQLNRQIEKYADIYTSRVSNFLRYTPYSYFRSPAQSLAHDRNLTRYYEKMYGTAENGSLMGNSSDSSSNGNDSISLKGEGPDSDPEHEQDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY32</accession>
    <name>A0ABQ5RY32_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004927</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF169">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SBP-type" evidence="5">
        <location>
            <begin position="429"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="295"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="493"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1213" mass="126580" checksum="911E99DAF85DDE0D" modified="2025-10-08" version="1">MDLASLFSEEGVGSERANSITSSDLDPFILQGDDQTWAVEDWNWDSMNMCAAPKETPNVACCQALKRRKVADNISGLAAAQEQSQQQQTHTQQQVQQGACCHGGNPGMHRAGGEPPAAAAACCSFSANQVQAMQHLAAPLQAAMNSFAPQRIETCAASSMCHGPGSLVPPQPMPAATVITTTGQPHMVGGGMGVGMPGSMNWPGLNDFGNPAAAAAAAMAAAAAAPSFFNSMTGNAGQLGNMGWGPHTHAQAMALPLPSMPVQPLLGPHAQYVPAVSMPMSAYPAPSGVFQQQASMALPPSSVPSPALGSSGETSSLQGSADNGKQLGCSGIRHGGGPTCSGGRQQADSSNKPTMLPPNKAQQMGATGGRGAGGADLSDFAMSESEDEDEVARRGPAGCNRGHGTSQYHQHHFLSDVVALQDVGDDNGLMVCQVPGCGKDLSHLKEYHQRYRICDVHIKLQQVLKDGRLQRFCQQCGRFHDLTAFDGNRKSCRDQLSKHNARRRRRAQDNAKGKGNAAAEPFGMGAVGGGGATGAATGGTISSGGSFEGDVGKLLNCLLQNPTQLHALRLLLGVPTHPALPSAQPALGVSLGVGSPTDSSSDVSDQARTYSLARDILAGRNGFAPAFESEHRLIRLSMKLFNRTPADLPADLRNQVTSWLASAPAAVEASIRPGCVFLTVQMLVDEVSAAQASEPGSLRSFVQHLLTRTGCPFWHTGTYTVQLHEDMLLVRDGRVSGDTSRGRSDGRFPVIRRMGPLAAVAGQPTTLKVHGQNLDAPNCSVILRYGNKHLHAHLQPLSSHRAIVRLPPLPELCGPAWVEVTRGACLSPAKQLLVARDEALVDEINKLDVRNGPLDQETVELLLQDMAMVLQHIAGGPGAGSQLPHAAIALKARRLLAFACDMGWAAVASAVLPLACASCSCAAEIVAAIHAASVPSAPSAATGDKRGLTLLHRAVRSGSIPLLAGMLAWGDSHGYRWRVDAAGPAGITPLHLSAMLDDARIGLLLLDHCGWPGAFTHLRSDGGVTPFHLAFQMGHYQVDSLMAALGGLHKVDQNADANNAASGADTTGHCAHSSGRRGRHGASVAPGMGCKLDDEDDVKPLLLQGCHGHKDGAGNNNGGCCADLDPCEMCHCTLPPLLLSIMASCNECGRRRMCMDAECTAAAGKCGACSCGVARPTRRSETGEVCSSRQHSTIFSITALCQGCHGNRVLAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZE9</accession>
    <name>A0ABQ5RZE9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_005863</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19821">
        <property type="entry name" value="Bbox1_BBX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010402">
        <property type="entry name" value="CCT_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045281">
        <property type="entry name" value="CONSTANS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049808">
        <property type="entry name" value="CONSTANS-like_Bbox1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319:SF77">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51017">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CCT" evidence="6">
        <location>
            <begin position="323"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="390" mass="42143" checksum="C4E519A67C835910" modified="2025-10-08" version="1">MACVVCAAPAGVYCENDKALLCKDCDIRIHMSNAVAARHVRRTPCEGGCSKGASLFCRCDNAYMCEACHCANPLSSSHDTEPALPLPLLEQEVAIAEQPNVAEPCESVAQSAASPVSWFVDDEKPSLGSFEDTAILSPAGSEAVVPVMSAPLDDFGYIAPATFKEIKDKLEFEAFDLDNSWMEMNFDFTDILSDGPSDAGLVPTFDADGAEVVADAMVPSFPEEMLIQENDADTEQSCKPAAELSEEEPAAKLSMVADVGNMAIYGMQGAFQLPQSASLFFQSAIPQSAVPSMPLPVIVPPVSQPKNTAAYNAVLAAGANLTREQRVARYREKRKNRKFEKTIRYASRKAYAEIRPRIKGRFAKKEEIEAWKAAHGGDDAVVPEVLDADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZI8</accession>
    <name>A0ABQ5RZI8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="313" mass="33471" checksum="289411F48BC7CC3F" modified="2025-10-08" version="1">MVTYLDTRSCSCSFRFLVWKERRLEVTDMANNVPCNYCRAGTLLLLISVFWALLCKLGEGCYSDHHFWNESLNPLGPGRCDPRRKGCDCDGWRTCSPDGDCQGRARSGLNSSSLLSSSSPPPPPPSSSSLLFNNPPLLPAGARQPGEAHAPSSDNPIRDVAAAADLQSGVSTGPPSPSYMKRRQQQGPPPALLAPKNGTMQNHYVQQQQLANITNKSGGDNNDLRDRHSNIAIIVSGTLVIAGVLLFGMYVLWKSMGKCSSLSSLSSSLPSLSPPLRSLSSPSSPSPSSSPLSSLPYAVCSCCVKVYASLQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S025</accession>
    <name>A0ABQ5S025_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_005568</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="288" mass="31608" checksum="6256012433891D6E" modified="2025-10-08" version="1">MVLYLIGLGLFNEQDITLRGLETVRRCSRVYLEAYTSILLCGKEKLEALYGKEVVIADREMVESRAEEILEGADTQDVAFCVVGDPFGATTHTDLQLRARERGIAVRVVHNASVMNAVGACGLSLYRFGEAVSIVFFTDTWRPDSFYDKMIANRRLGLHTLCLLDIKVKEPDLAALARGRVVYEPPRYMSINTAIEQLLEVEERRKEGAFSCTSLGVGIARLQADDQLIVAGTLKQLLEVDFGAPLHCLVLAGDLHVTEREMLDFYMIGGSGSPSASAGAEQDGDAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S044</accession>
    <name>A0ABQ5S044_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005991</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="115" mass="12972" checksum="DDC0EF165CE0817E" modified="2025-10-08" version="1" fragment="single">RNVSGNRVMAFRDAHRAQQLLETASEFVGYWMMGRQEGPTHRVMEGLNLLSASDIRIDIHGLLVHTQDMGIDGLARLLSSEHRPAGLSLKHWHSRVCAANEDTDTVFCLMEMRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Y6</accession>
    <name>A0ABQ5S0Y6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="250" mass="26034" checksum="14E888E8BB59345E" modified="2025-10-08" version="1" fragment="single">GVTAAAAAASPPSPSRASRLHQQPSSSSSSAALSGGQTLSSSRIKKTMVALGDAHRALLSQATLLTEVFPPGLTTEQLCIRAALRPATFGAPEVLRYLRHHVHKMVDVLSALPGNRLEMKQEEEDVAAPGSGGNRNTGNNRNTNNCRTANGQHGTPRSSMTGQTPRAATSVKSRGGGGGGAPAEEFDPWGLLGDLLDFVRWLRGLDIKSLESRPDLEGGLEQLSSVVGICDQETRRLLGRVGGTVAGNGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S273</accession>
    <name>A0ABQ5S273_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006316</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03025">
        <property type="entry name" value="Katanin_p60_AL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027497">
        <property type="entry name" value="Katanin_p60_AL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF78">
        <property type="entry name" value="KATANIN P60 ATPASE-CONTAINING SUBUNIT A-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="327"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="104"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="229"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="542" mass="58118" checksum="79D65BE518E6D392" modified="2025-10-08" version="1" fragment="single">MSELAALKALSKAKESEEKRLQERRRNVLVLILRHLADHGYTDTYEKLCSESNLNLQKVDVADNIDLLRVLQEFEDSYEIKFGKRPKVVRRLVEEVVAGGGGIRPRAVSATAGGSTGSPPAGGPLQRPIGSGGFEGPPVSGAVAARQRREAGAALTAAAEATRKGELLDRRQQALNSVKPPPEVGMQRIAGVAVDGKGAVLSAGAGGGAMSREEVAAAQLQGLHVAGQAAHLSAGRPGGPGGAGGPGSDSDNDPDSFFERRVLKPLPPQLQGELRDLGNAITRDIFTDSPNVRWDDIAGLDQAKRLIKEAVVMPIKYPQLFTGLLAPWKGVLLYGPPGTGKTLLAKAVATECRTTFFNISASSIISKWRGDSEKLVRVLFELARYHAPSTVFLDEIDALMAARGGEGEHEASRRMKTELLIQMDGLARGNELVFVLAATNLPWELDMALMRRLEKRILVPLPNTPARRAMFATLLAGRCAVDVSMDMMADRTEGYSGSDVAVVAKEAAMRPLRRLMSRLELDGPVDPSQKVELGPITVDDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3A2</accession>
    <name>A0ABQ5S3A2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF18">
        <property type="entry name" value="ORC1-LIKE AAA ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1165"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1258"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1562"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1967"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2058"/>
            <end position="2209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1166"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1302"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1999"/>
            <end position="2008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2063"/>
            <end position="2073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2090"/>
            <end position="2112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2124"/>
            <end position="2147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2159"/>
            <end position="2182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2183"/>
            <end position="2192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2209" mass="232185" checksum="9657FD1A7E5452D8" modified="2025-10-08" version="1">MESELKSRRRSARLRKDEYAENEGVIDERCILGRCGPFNWPKFDLSRPIYGVRSFARWSLPKSCGPLHAGRPTLVPSFTPRNHYFTTNEENVYEVDNVEFLKDQMDWFQSMFGDSHIPRSLLRSLLQGDIASQVEVPTAQRRTNLALAKAAASGFPRYDRLAEELRGNRLAAVPLPGGVTAVFSATGQIGEVLALTLLRPSPEPAEAKTVSEQPNDGGRRGAAGDGVPAHAAEQVAKVDEATEEPESPPWHPNSYCRTPPVSQPGAAAAEAHATAAAATAAAGGGGGGGALHLQAFPAAQLSVNKTIWEIAVKEHKTVKLPGGLMAEQNRAGGDAGSSSRFGTPRRTSQSTPAAAAEGVKGEEGPMSGADGGSWGEGGPAVWVALRSNYCINVLLSYQVDAKDWRSWRLEWRASHMTQRLIAALAWNPHLPELAFMLMDGSVFIASTHAAAGGVAASLRRLAVRQVLLPMPPLAPYGRNRVPPPPPMKRDPVPAEPMGFLFGKGRLCVSYGVHPRTLLVARGRYLLKVELKPRGQRHKCSLLHYLRKKEGCFWGLATGASLAGMQLSAAPHQLVHLVVAVGRRDVILIDPRRTANVVLLAITHGMSKGQMPNVMSLWVGPWFTAAVAAAATAATTATAATAAAADGLENNGDVEMGDAAADFATAGGGGDFRGFGGGGHQYQISPPPQWPLRTSRHAPRADSDPYGMYGSQKEIAGKSSQQCSRMFDFQRGSQQGCLREEGQDQTNKWMSKLQKLSYFSKGLFGPNHAAVLERLAKERQDKKEANPEGADARAAAAAAPVVSPTHGSVHLVVLLANERSGDVLCLEVVLEEPMSIQVEVPQLPTGDLNAHALRDARLPCCRVQTPSPPVDDAAAVAAAEAEATGRHVGERDGGGVAAGSSIRHAGYIAGGAATATHGSGWRAPPPANLDTAVWRPNCLAHMHLLGMPYPPCNTVREDVGQDLVTALLGARSAMYDTGLSVQLIAPYTTSSCSSNGSVATPADHAPLLLMRLSYDGDLLLQPLQRLSPGSILPTRAEHGSAAGAATAVSGTTDRSRNGAAVAAGGASGAECAVADVDRIDLKVEVLESPFILADLRSRLSPADYIQLLKACGANNDAQAAINQLEPAVHNHQRLDPPLPKQQRDKLDFSTHYIHLASMLRWANRPVVSEERPSRVGEREAETGGGDAEPQVQPRKRPRLRRQLYDDAEEAKVELKEGQEGKRLEKKGYKQTYGLAAEAAGDGTKSPREATLERLLPQGGSDAAVAGGGGGGAADIGPTSVAAVRGHQDSEDDRDPYRGHNDMGYGGDGGSGGGGGGRAHVHRVGERQPPPLSQAEGRFGSGSQPFMATQEAPLQARRRPTFTWIHNKERPDIAVRRISQGVVVVHHLRPKQMPDKVWALTAGPSGAAGPSMATATATATATATGAGRGTAAAAAAMEVDLQRDAGGSGSGQLAPPPPPSAAHIIRDAVRQRLWASPYPTLITDLSQSLTNATQYYKENPLTSERSFRHTAWFEGLRGKRRFNLALRASRRAVAERLYNSGALQGGSRPLPSRVRDTAKELARSLSYDNDGGDRGGGGGQTTESSTAAAPPPDLWDIEYQELALMALIVGELAEATKCTADDGGGDGGGVGGGASTSYRTAAVAAGGCHDMADAPRLSYRALGHPIPARHCNVLNVNANVEVTVPPVASVEEATAQLPTPPITLRLRTRARRGTDAAEAAGAAVASSLADAAVHGGTGAYGIMAPPQNYRCRRLSSSGSSGTVGEDLLFLHSLVAYQPAQLRSRVSVLIPEQATEDDEPPVADLPRYVRGLRYAQRAEMVKANCLAVYDYMSRRRHRLTPTPTPSPTPTPVPMPASASALTSAAATVPGSIAPVLTPLGACMMENLLAEWPAYDSTAATGVVGVAAGVDQPRILNAPDHLVDRALRLRPFGRYGDEGQNEKRYVAHEGRRRKRPWRHFPFFHMEKFQGKLDTAEDSETAAATEKGQRLRAPADGGNDTSAAGGGGGGGGPSTSALSAEARESGGMKSTPAVAGAPTAAAAAAAAGTSDGPASRLLAAAVGRRGGSRSQTLTEGQSAATAAEVGPPPPPPLPADAEAALQGGSQAATQQQSAGLLKSLLGMSVSTKQQSALPQSSQQPVRQQGQRPPVVSYFSQGGEEGEWLSLSQPVLPSQLSQMRSGPARAAAPGGGGGGGARAGQEPPRPPRPRITEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S450</accession>
    <name>A0ABQ5S450_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF444">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="218"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="356" mass="39147" checksum="1FBD8A69FF49A25D" modified="2025-10-08" version="1" fragment="single">MSEQLAIYRRCRRQYPGTAACVLLPAFCRRKAEASLRGMRHVHRFEPGTVLFNGVDWEGTLAPWPCLSYSVDVWYEAPDGGAVGYGPALSALTLSAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVAEVNALGLSPESSDCPQVTLADGGKHPIVGRITLSLSLGPLRVVTRPYVLQGLAETAPYIMGSSTLRQYRARIDMETATLHMCKGTLSIKVRPSTSPYSAPILFVGKKDGTLRMCIDYRGLNANTVKNRYPLPRVDDLLDKLKGSAYFSSIDLQQGYNQIRIAASDIPKTAFRTPFGHFEYTVLSFGLTNAPATFQAVMDRMFRPFIDRFVVCYLDDILVYSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4B7</accession>
    <name>A0ABQ5S4B7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF190">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="375"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="436"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="511"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="847"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="517"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="853"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="869" mass="90183" checksum="F7207AAC3BA6F799" modified="2025-10-08" version="1" fragment="single">GNGDICPAAAAAVVVMPAVGYPGAALGPVASSPGLSSPLSPASRILGGLREVLLRLSKLQADICPALTTDAIACLPNLTDLTLDTLVAPEALEPLGRLRCLAVKKLANPEALERLTGLTALLVNSDSSCASEFLPTCTRLPELQSLTFKTSSTKVLQQPDGLCWQGFSRLSSLDLRAPVECLKPGLPAVAELAGLRSLSLDLYCASDEELMVAVVAPPNLESLLVSSDRSRSGGVIDVHPNPRLRALRIRLYWTALNLVLPEGVPEPSPLPEPLSEDLDPEPRVWNPDQDLKSPLCGGPARADTTVVQRQLPAAIAAEEITEAAVAAAAGPRQPQRVTDGLDLDLECPHADGMDVGIVAAGRQEPFRTVTAAADACGGGLRRRRRRSDESPSPFSVGGGGGDGQAGHGLGAAAGGEGAAATAAATVHLMERGPRRRIMSTGDDGAAAAAVVEPSRDGRGTAAAMVAGGSSEDESGGAGGSDGGSSGEEVGYRGAGVSGASGGVNAAAALAATGDADRSDEDVDSEEPEAEAYPYRRCAPTAGGGGGGTTDYNPHASRRQVPPRSTRQEPPAAGSGSALADGTPCMECRRRRRSAAGGAGISTPAASSVSASAAQVPEMELQELCLDACFFLLYGSLVPLLRQFPRLRKLTLNNGVALENGELAQLCELSSLQELSLSGLHLVTDAGLKYLSRLTRLTSIRVKGFRKLSLHAAQAFLRQKPIVRRYEEISGTFLMSLAGLSELRYVALKHLHTIGEGALAAGLGALKQLTRLEVKDVNSMSDKVLVALSGHTGLKDLSVLHCEGVTSRGRNAVVRALGPRVAVDFEGCKLPDITRPCALPTSSTKAQLIDDDQVSSSNQQRRSVQHSNQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4H7</accession>
    <name>A0ABQ5S4H7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007594</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052920">
        <property type="entry name" value="DNA-binding_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358">
        <property type="entry name" value="ALPHA/BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43358:SF4">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="95"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="816"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="371"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="607"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="841" mass="86934" checksum="D8A615B4796FAED1" modified="2025-10-08" version="1">MPGLGALWDSLVDCICRPPRDEYIYPDELVGGSRRLFRVGRYPGIREDLTLTNKRGLLLQCSHYLPKHVRGKDGRLPCVIYCHCNSGSRRDAEEAICVLIPCGVSVFTLDFAGSGLSEGQWVTLGAEEVEDVATAVEYLRAGGKVSTLGLWGRSMGAVTALLYSHKDPSIAGMVLDSPFSRLTDLMLEIVQEQRLPIPRPLAKLALAAMKRSVIKRAGFDITKVSPVDAVSQSHIPALFGHAIGDTFIRISHAEALHEAYAGEKELLRFDGDHNSKRPEFFYNSVSNFWHRALLLEHLLEPADISLSSIGPAALAKEPGGSSAARGGAGRPLRPPPPRLRHPQANPTLTAATSADASTAATMGEPLPPPEAAATGTAGADASRVVNDGDGEEDEGADVAAASKRSAPWEPRLWELDADKTARERQRFRRMAGGLPSPSPAADVTLDDRAGSSFRNSAELARLFATASDESLAARLLGEDPQWSDKLVSEQGPGATAGTAAPVNGRLVKSLKAAAMLLPATEEEEEAQLRAALQLSLLETTAVPPPHMAARDIGGCAAAGSQPSRQQYSQEQTAGEQMLSSVGEASGCRSRSGSGIGNMAGSGSTLRSGQPGPAEGAGSSGSGTLAAQTSVATSSTTSSTSPDMELAGPVASDAIKPPSVTIVEATAVTAIPASAPVLPPIRSNSEGSTRAQSPLEVIPSCALSGPPRSAQLIPIDDLPPPPAFVAVAAALHPVDLMDSPATATAVSAVAAAGGNGKGLGSSRPSWGDGDASAVQGAAAIAGPGMESMEAAGLSLYDLYTEDELMALAMQLSLQESATSTAPAEPSGAAGTRAAASELPSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4W6</accession>
    <name>A0ABQ5S4W6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="694"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="820"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="874"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="940"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="419"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="700"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="717"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="912"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="969"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="980"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1041"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1054"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1094"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1117"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1161"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1200"/>
            <end position="1215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1233" mass="124734" checksum="23D614EFAAA2194D" modified="2025-10-08" version="1">MGRFHGSLAEDLTAEAMIHGWEAVCKLWEASSVLDSCDLDGPAIDTADKIVQQHLRDALKLFETALDKFSCKIVYDIVGHAYNPILHELDRSDPEVEAFRQGSIGTLGGAGLNDLLCGAAVATVVRPGLEVTGIMRARPRVWVTLPSTRGQQTPQTGIAPVPAQHQQQLRFGSFAFKVPVLGAKVSSNCGQAQKSEAPRTCSDAVQIGNVKASAAPSSGADAATCHSEEQSGSVAQISSRTISPPSPRLAAQPACGASSRVSEHSTTQHGDLEQHHGAEESMGCATSQESSPAYVAATPIASGAMPLLPQPPQRPVASFTGAGPKGPLQLLLALRTGRRTGPVAVPATITAVDTKEDNPLSFRTGQFGTGESAGSELEAAVAGVGDRKTSKRSSACSVPAVKESPTGRLRKGTGAVETSGPRSGAAGARDAGSPGGGSHYRGVGSDDVDNDDDVCYQQYDTSTHYPATQPRQAVGSAVQQSQAPQGRPHYSAPVRAATTMVAATVSPPGAGSTLSANTVPRLAVATCGVSSSIGGGGQAGPVSSSERPTTACPDGRCNPAGPIPGPSLDRSASTATTAGAAVRADIGWNGGFESVKESPLGSTYAVSRGAPEINETSGSASGRDRTSTCRNGAVGAGPTRRSDSGGNGGNLNPARSAATAAAAAAAAPCGVPSSAALAAAAAVRAAVAAAAAANSRAGLPSSTALPSTTPRTGAVRAAPQPSPSSSATVIPNAGSEAGAGAGGDKDAAAASPPFAHTSNAAPGLPSPESKASGVEPGVFAGGTSGRPRIEAVPRRTPMPGAGKLAAHDVASAAAVAAAPCSGPGSCSGVSQGHGPATSRQGSRRQSETVLVAHMAQGRRFLAAVEAEVGDEDLGMSQPTLATPPTGHVHKTTSRSSGADLSAAAAARPPPPPHHHLFPRRQRSSRTLIVTATAMGNIDVALEPSRASGNPGRGAGRGARASRDRRELNAATAVTTTVADGGDRDAARELLRQQRHISRGRGRGSGRGTGLGIGQPLPPPSSKQQRPEAESCPQPNQQQQQQKHEQQWQHHDYQPPRLQQQRQQQEQHMGRTDRLSQVNNAHQREHYERHPPSDAPRAGGKAPPAHPHSAAAKAGVLKPGGGRGGGGGGDEVSAAALRAEESSEEASVRTARGVGGAGACGGEDDDAKEDEEQVEGEGCGDVQGAAGRRGGRGGGGWRGRWGLRRRGAGGGWRRRGAGGLVAGAGPTSGRPMPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5A1</accession>
    <name>A0ABQ5S5A1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008491</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046732373" evidence="2">
        <location>
            <begin position="17"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="366" mass="39085" checksum="9CD95AFA1B75B4D8" modified="2025-10-08" version="1">MASSIILTTLFALVASSVRPTYDVDPGTRLSSKFRGQATTAAVERRLQATTSAHQCLWTRRAGCELNADFVTTLSGSPTTESQKLLARNAALSNICTGYNSSYTCLAKPGEYCVWSSSYQECYLGTGYIPDEWLQSRLWCSGSLAETTVKCVSSITSSTCSANAKCVWAPTGLTSQASSIVGIIVTAILGSSNSSCVPKWATDEKVVNNLVQNAAKGTTVAEILAIIFNDLMGTCSSAQKFKTMLTTCPVASASTCLSTPGCAFQSNACNYADDFPVYLLLNQTDTWVVAWNKARVQCEVLSSNSSCSSSPDLITINKTLYSQYLALQPSFPPEPNVGSLSGPTTAILLGLFFSILHLFLFYGKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5M8</accession>
    <name>A0ABQ5S5M8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_008105</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03572">
        <property type="entry name" value="ENTH_like_Tepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035802">
        <property type="entry name" value="ENTH/VHS_tepsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039273">
        <property type="entry name" value="TEPSIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514:SF0">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="6">
        <location>
            <begin position="2"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="817"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="863" mass="87709" checksum="0E84DB899079D0CB" modified="2025-10-08" version="1">MARVVEVVQIFKKVEDVTAAKEDPSPVYLMDELAEMARGSAESSEKIADRIAKRLQNKSPVVKFKALRLIRHLVNKGCAQFQRTMQRHASLIREHTQFKGEPDPFKGDTPNQRVRDAAKEASEALFNTVMAQPVTSLGSRIQGFGSSAAPPPSSGSSNNSVSGSGVSTSSSRMVGFGSSSYSAPAGPTNNNASAGFAASSPLQKGVNRPLTGSYGDAGSSGTATGTGTGTGSTGGFGAGGYTAPAVVATEVLGSEAQLVDSICTPGGLRLAPGPEDLRRFVEAAGNLDGIKLAELLRSKMNGSGPWQQQLRALYALEAVLVQGASQACGEVAVMFQSDPGPLQAATGHTQAVVRDRASRVLKLLLGNDSVATIAPLAAARTAPAPTPAEPAQMTADLLGGLDMLGGQVQAGYSSHQQQPQRAGAGATAGGADDIFSGLTLGTGTSPQQVPIHQQLLHQAAGVSNARPEFLDSQQQQQAAPVVSGSHLVVDPFPRDMLHPSIASHPQAAYTQQQTTSQWQQQPQHLQQAHVARLAVGGGLVGTPEPIAATTMGPGPGAPHGAHLHTSDIQPKQAAGVRPPLDDMFADMSISSNSRVQRPVQGVAAGSEYGGMGDAMGGVRPAYGGGIPGGGTGYGTPTGPFGLTSVSQMSLPSSSGVAMMYHPIGAVQCTAPHSMSDTGSSSLPHTQAQLPQQMARVAVSTHSATVSTADIRCGTDLLGPGTSEAVLGKGDGAYGPTGVGAGAGPVGGLGMGAYPAAGGVAPSTLGAGGVLPLGVAGSGSGGMMVGPGGQVLYVGSNVQPSTMMGPTGNLGPANGHLRQHTGSWQPPHAMQQQQQQHTLLSGVPNPHQDLRGGAAFDFVREQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5N4</accession>
    <name>A0ABQ5S5N4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Plastid acyl-ACP desaturase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_008416</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01050">
        <property type="entry name" value="Acyl_ACP_Desat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005067">
        <property type="entry name" value="Fatty_acid_desaturase-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31155">
        <property type="entry name" value="ACYL- ACYL-CARRIER-PROTEIN DESATURASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31155:SF9">
        <property type="entry name" value="STEAROYL-[ACYL-CARRIER-PROTEIN] 9-DESATURASE 7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03405">
        <property type="entry name" value="FA_desaturase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000346">
        <property type="entry name" value="Dlt9_acylACP_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI64991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="45007" checksum="4B18C2C228DC992D" modified="2025-10-08" version="1">MMQVGQPIAQRRGVCKQAALSQGRAVVVRAAVASAPQQPAAPSTFTPRVQGPIIMNGQVLHSITEAQLDVVRSLEDGYLQTQVVPLLKPVDKCWQPADFLPPSEDADFLDKVHALRERVKNLPDDYLVVFVGDMITEEALPTYMTMLNTLDGVRDETGASLTPWAKWTREWTAEENRHGDIMNKYMYLTGRVNLKAIEITIQNLIGSGMDPKTENNPYLGFCYTSFQERATKVSHGNTARHALEYGDEVLAKICGSIASDEGRHEIAYTKIMDGLFERDQNGAMLAFADMMRKQIVMPAHLMNDGEHEQKTGRNLFADFSSVAEKTGTYTAMDYADIMEHLVGRWKVPERTGLNGEAAHAQEYLVKLPDRIRKLAEKAAARKAKAKVVHSPFSWVFDREVPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S663</accession>
    <name>A0ABQ5S663_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAM192A/Fyv6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_008605</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039845">
        <property type="entry name" value="FAM192A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019331">
        <property type="entry name" value="FAM192A/Fyv6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13495">
        <property type="entry name" value="NEFA-INTERACTING NUCLEAR PROTEIN NIP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13495:SF0">
        <property type="entry name" value="PSME3-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10187">
        <property type="entry name" value="FAM192A_Fyv6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM192A/Fyv6 N-terminal" evidence="4">
        <location>
            <begin position="66"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="222"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="288"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="314"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="329" mass="34420" checksum="E6FF9486A14F9DAC" modified="2025-10-08" version="1" fragment="single">MTEERRVDMLRLVGEERYYDREELLGRDATSVFPSLARQQQQEAAAGGLAAEAVMEALGARKFISESELETIRATRGATVDDGAVAVDKPLAEILRERKVAKEAEHADKWKQMKIGKNRPLDADELQFLDSVAAAEVEKERAWSELERAELEAFKAAVRERSVEPEGAPGPAPLLTGPAIKTDPRVPSAVTVSVGTAATLAQPKPILKPLVKVKPKVAKEAAVKDPPATAGNPASAVASPGAPGSTKDTVKSGEPPSKKTKTAGCNGSDELGTAESGRVIRRQVVGTAGAGVVGTGVSEAVPGGQEALRGGGGDGRDEENLGAHDDDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6E5</accession>
    <name>A0ABQ5S6E5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_008808</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF453">
        <property type="entry name" value="ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1109"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1152"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1237"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1337"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="125"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="718"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="1109"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1430"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="425"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="546"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="661"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1029"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="546"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="569"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="602"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="614"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="693"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI65440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1737" mass="186952" checksum="EE92F7E283FAD8B0" modified="2025-10-08" version="1">MLVTAANAGSPAESVICYNDVPLRSRIFFSYVNPLLKLAATGCLQQEHVQPLAAEEDEPQALAECFYQTYESAKAAYTPRPGNSSPAAPIRRPWITYTGIALLRLYRWSMLLESLWALAEVARRLGTPVALRQFLRWLQAYAARESPPQWQGWVWAVVLGACGYSTVITSHQMFWIAARIGFSMSQQTTAAIHRKVLRLNSAAISNFSIGYVINLASNGVRRFDDAMPYWVFLWAGPLELSMVLLMVSLELDFLSALAGVATSLAMIPLQSALVRYIGGLRRNTARCTDERVRLAGEVVEGALAMKMLGWEGPLEHQLRAIRSREVSFTRRIQRIRGIHFAMQFCITPIVAFVTFAVYRTRKGSLDIPSAFYTLSLLYLPKMSMANFFVLAVQTLTELRVSLQRIDNFLATTEPRCPATVAREEAMRHAREEAARQSKRGGSRSISSRRILSCQTGPGSINATSRSSSMTSPYSVVEPLSRQISNAIGRGLDMIIRLSMRFRVAPEPAVQRSAWTEWQAGYVAMRGADYDWSRPFGFVRPVGLESDREAAEAGAKENGRPRQQQQQSMEKVRGGDEHIVGRKGCGPVAEATTSDETASGRCSGEERIRKKPNDAATSLESGQITPRGSFPVVPYSSGTIPVSDLSPSDTLADDVSEVFEEAEMRDEVTGGEVETAGNPMATPSDPGEVPRPASGGGGVGVGGGGGKVNDELGGGDNKTRSDGLADGATDTHGSCSGGLTLYGIRFAASPGELVGICGSVGSGKSSLLAALLGEIQPLNEEWEGQDAEEEERIGERRTKGRAGGQWGAAAAVGEAAFLPECSDESKVIGSAKGLICRSGRAGGSAGGGCEAGGRGPIVCGRVAFCSQIPYIVAGTIRENIVFGAEWDDQLYERVVHACCLADDLTALAGGDLTQLGERGTNLSGGQKARVALARACYARPEVALLDDPLSAVDPRVGRDLFARAIGPGGLLDEWGCTRLLVTHQRQYLPKCDRVLVLRGGRLVSEGPWRQLVGMRLPELTAGQGALMGQVAEGGGGKVGQEGDQPAAEGEGEQDGPNVDEVLAVCRTSARAVDETGETAWTMEGDKLVHSEDRAVGAVPWSVYGNYCRRLGFPTTLAILTAIVLGQGVFMLAEWWLSRWARADIYSQRKIRWLWVYGMLTGIVIILAFVRSAIFFEATMTTATSIHNSMARRVLRAPLSFFHTNPSGRIANRFTKDQGQVDDLLPLCLFDALQSIFPALGAIILVSLAVPVVLPVILLLAIVCYWVRRRFITASRDIKRWEAVTRSPIFTCFSTNIKGLSTIRGYDAADNFHAAFLKLMAQNGSWSFSYHSLSRWIGVRLDAVAGTILLLTSLLAMAMRDRVKMGGIALALTQTVQMTGLMQWVVRRTAEVENTMTSVERMLSYTELPSEAPRVSEGGGSPPPGWPKSGALTYEHVTAVYRPGLPPVLRDLSFSLSPGSSCGVVGRTGSGKSSLMLTLFRLIDVVQGRILLDGVDTSAVGLDALRRQLAIIPQDPVLFSGTLRSNLDPWRAHGDEALWEVLQGVQLRGAVAAMPGGLDAAMAECGRNLSVGQRQLFCLARALLQDAKVLALDEATANVDRATDQLIQSALRDFAHRGRETQGRVLLLIAHRLDTILDTDQLLVLAAGQLVESGRPGELIAQGEGVFAGMAAAAHMGAVRSAVVAARLVTPGPETVAGAEVAGGVHLVREAPATLVGPAGLEGGRLAWGNDAERTSRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6R8</accession>
    <name>A0ABQ5S6R8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="105" mass="11529" checksum="162D04548DBE524D" modified="2025-10-08" version="1">MSCNATNDSLSYIKDEDGNYPGVAWSGLARPGRATLSSLRGEEGIAPLKQSPEPQSAEGGRFPGANQIHLRISHSIAFFCVCEPTRAHYLHFILMPREFQVALKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6Y3</accession>
    <name>A0ABQ5S6Y3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="905"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="966"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1034"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1130"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1183"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1248"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1003"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1188"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1203"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1259"/>
            <end position="1274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1274" mass="124294" checksum="FA26AD23AAAEAC4E" modified="2025-10-08" version="1">MAADDAVSLLRSLKAAGEKTGAVEAEGALHLRRNSRRTMDGIHDTATLPQGGPSLILPMPKFPASASPTSNGGGGGGAALISALSFHSASVSLPMANSPSNCALSSTALSPGAGGGAALATVGSTGAGCLPVIASTASSPTASPSRIGVRTSLPSPTVSSPGGTAAGGGGPPSPPCNGAPHPSAGLQRRTLHAHASNASPHDALQLPHSHGSPLPLLFDPPVSAPPGLGAAGAASSPLGAGGGPGSSSPVSPGSRQLQQLSAAAMQSTPSAASSLPSITSVGSGGGVSNGGGLSPSALTPSLLPPATVRSVSARNSLGGAVSNLVVAAAGGGAAAGPLTKSHPAVGSPSAITPVGAIAAAAAVGGGGGRSLRAPPMPGTPFGTPLPPPIASTSAMPPSPGSPSASVTSPVPCLMPSPGTKNSGGAAFEPGQASAMALPATASGGAQALMQLQLAATAAAPPPLHSQQQYSSARPSSARSVAASCTVPEGLNGTLSSRRLSMTGAFEASLAAGSSQKLVLAAAHELQHAQMLHLNITQNNSHQPQQQLQLQQTQQQSQQQLLQQLLPPPSGRLTLTSARGPSVTLPGDTTAGDGGSVADANGIPRTTGRETRSSDPGTCGQTPVAAAAAAAAAQSEFGFVVPEPAPAPGRPSLAPLPSAVLQSVRSAQMPPAAASASTASLPPTAGIGWPLAPVHATSSPQLQLQLRTPRPEPLLQRPEPLPPLQAVSAVAPVRAAAAAVAAAPLKTTPVPLGRAGTLAPLMALDELPSPFTAGPVAESTLDSRTPDLDPAALFGSGYREGSRSGSASGSKAESMELTMRSIAARQGASPGTTASVIDKCALQSSIRQRLQTDPLQFHPLPTSAVRRHSGGSVYLDPLEATPKLVLGSGGGGAAAAAATIAVGPPLRTGHADSPKPVAAPGGGGGALWAPSQDILPHMLSRGSSSSGSGGSSRLCFSPSAPAAVAFHPVTPGLEPSTPRLRSNSSSAVDGFSAPSSAAEKPQHQHLPQQQQQQQRAQTGLQSNYLQALAAAAGLGAGARTGDNRSEGPTLRRQRSISDSGCGAFLAAAAAAADSGPVGLPLEGDLAVLGDMWARVQAERNHNTRYNLTDAAAAAAGEVSGALDDSLVASEQTTDAVPRAAGEGERKGPRAQQDAELNTPTAAVSELPRNLTGLTSLSLWAEVSGGGTGHTRSFTRSSSGSPRSYGGGGGGGGTPLRGNAGAADDARGQGRLDASVSSLSLKALKAATATATATGAGGSGAAAAANTSGAAVKAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S714</accession>
    <name>A0ABQ5S714_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CobW C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008724</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051927">
        <property type="entry name" value="Zn_Chap_cDPG_Synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603">
        <property type="entry name" value="COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603:SF1">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="2">
        <location>
            <begin position="33"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="709"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="833" mass="87697" checksum="FF639737D9A35982" modified="2025-10-08" version="1" fragment="single">TTAASNRASGTQALLTQNSMNGHHAVRSAGTAAASAAVEEPATVAAAKSAARTAAFGQVLRSKGFAWVSGRDAHVGEWSSAGNLMRFGTGGPWYDVLPREAWPSEPDKVAAIEKDFLPGIGDRRQELVFIGIDMLRDQLVEQLEKCLATPAEEVELKKLAAAAAAAAAAADAEAEATDSAPGKKALRAPCVLDPFLSWPDIRDIMDAGDDDDNDDGEEVEEEEDVVEEQDEEEEEVAEDEVEEDADEESEGKSDDEHPSTGHGPVGRWEPGVVLEDISDGAAELQALLDGTEQPAVLVLWHAPWAAASREAEAWFAALAARHPQLVFVRLDVTASQANEVLAISGCRLMALPASRRRDARPTLRCGAKWPAVTLHRPPGLQHEAYFTGATALAELRSELLRRQPEWEGAPRTVPASAAGNTDRDSNSRISSSTVIQQPTAAVSTTGGGGGGGCNDVPLLRWPTTDPIPATPAKPTVSVSVVTVPTAATASPSSSSVSSVQQRQLQLQPRVSELRRGAADLKAHLQEARDAGCPLVVMWLGAPPPNYPSTPTTSVPSTAGGGSAVATTTLSTPPPAAAHGAAVQHHPVPRLPSRSQQDGAVLELLELSEQEQAALRVDVAAACKQYGAAVRVLFADPGASATNRALAEALRVAAAPTLQVYVDMKMAKSFKGVAAVSKLAATLLEVAAPLPLLVPTPSAASAMTSQSVEMSPCSSLPGGSTGASELSLPPQARSSIYDPPSGKYGKPGARKQFGPRGRGVFWPRMPCLRCGCPWWLGEDWDAECVRCGWDCEADGYDDDSNPLPAFKAKYDTFTAALREGRTPVWRGKHVGPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7R8</accession>
    <name>A0ABQ5S7R8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-dependent helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF41">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE DDX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent helicase C-terminal" evidence="2">
        <location>
            <begin position="303"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="562" mass="57783" checksum="BBAC081D608BFB27" modified="2025-10-08" version="1" fragment="single">MRLVSSLQRCLEQPPQTQMSGQRSGDTAGATEGAGASGAVCSTGAEVIGTHEFLLATRMEHLNLFPLLSWVRESRLVMKVAGYDITSSGTEESIVPEAAVRLDDDKGKEGAARGTQSTAVEHEVRNGAASGGRATVAAGGGFGARRSSLFAVLSFLTSLTQPNADGRIVITPAASGVGSGSSDVATVACLRYTVLNAAAHFTSILQSARSVVLASGTLSPVEGLLAQLLPDVPPHRVRLFSCGHVVPPENLLALVAARGPTGQHLDLRYGRRSDPRISEELGRLLINICTAVPAGVVVFAPSFAYLDQLVAAWRRSDVWPGLTSRKHVFVEPRSTSAVEAVLESYAAAIKRTAQPPSVTAAAATSGAGAGTSAGRRPNGAVLLSVVGGKLSEGINFGDDLGRCVVVLGLPYPNPSEPELRERMRYLDEAAASSASHPAASTAAMDTGAAVTTAAKSTASGSGDCTKSIPWAAAVGARDAVTSFPSSGTSGCAITPSGTASAMMSDLIATGGNSPVFAAPASPALLPRLTGRQYYEGLCIKAVNQCVGRVIRHARDYATIVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7T4</accession>
    <name>A0ABQ5S7T4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_009320</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013729">
        <property type="entry name" value="MBF1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245:SF15">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1 MULTIPROTEIN BRIDGING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08523">
        <property type="entry name" value="MBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="5">
        <location>
            <begin position="85"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI65724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="138" mass="15161" checksum="AA58C1A087C10231" modified="2025-10-08" version="1">MNMNGQDWDTVVIKKKQPSGAALKDEAAVNAARREGAAVETQQKFNAGKNKPAQQTITGKPASKLEAETEDFHHERVSSNLKQQIVQARTAKKMTQAQLAQAINEKPQIIQEYESGKAIPNPQVLSKMSRVLGVVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8B0</accession>
    <name>A0ABQ5S8B0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008802</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF14">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="187"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="33783" checksum="6D48E4DC6BB58360" modified="2025-10-08" version="1">MIFLCDDINSSHTVLGVGRYATVSTPGLLDLPEPILQLIVASSGGSGGACSCAALRKAFQCAFACPELAAIFLIKRYGPNTALFHVYNTSQPRYSLLSGVHDGTDQDNVTNKLVRELLRMGASPRVQERFLLTAAAAAGDTLVVRTLLRAGVNASSCGGRSLVAASAAGHVAVMELLLRAGVSARAENGMPLRAACREGRVAAARLLLHHGADPRAAASSALVEAARGGHLTLVLELLASGADPRVDGSRALVEAAAAGHGCVVLALLVAGAQPPARNGLTLRHVAARDDHQVLEFLHASLSRTDENSGVECCRRLYSCLASLGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8R9</accession>
    <name>A0ABQ5S8R9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1251" mass="127841" checksum="8F7878B44B32E94A" modified="2025-10-08" version="1">METESQYPQQLHGSLEYETRTLHPGLLSLVEGRLRSLDPTSADPASTAEILSLLTSVLRNACSGRVAPDYVLGPQDAMQDSVRLACTMVSWMSQGGAGHVASSSPETCASLKDFVAAALDLASRAFFSPEWYQHLGPVQYMQASNAVRTYVRDLAPVAFDATAACWRAVEQQLASGHSNGGELLEACRALFDALFEGLKYLGTTSAERINDPSVPREMRSFAGLNILWSGLSKVMTALPPEALQQLLSGCAVYSVLRALLSYLVSEVRAMDASSPADYETRLKVSRFWLQHFARVTQAHPAAAASCWDELAAATAEVYGCIAAAGSAAAAAVAAEGQPNPPLPPAQQQQRQPAEFHHQYRMQMARDLESAIACKMASLLALVLDAGCLEEGRGCGRVGSGASYGVTTALPASPQQLEARLGKLEALARTGSAGCGAAGGSIQPAPTIEASDCSTVASESAGPVLLCLALLQRAAHVSPEARAVMVSRSLPWAGIMTAVNLYSMALEQCGPNAVAPCLDLPHQLAAAALALLAAAAEQQQVQGGGQDVETFAAGCTAVCNWGACAHPLLSVAAADVLSTLVQHCEPQLASSLIVAVARLAAVAAAAEAVATVAAAVAGGPSLGSSATGGGADETLKAPYGGVSGRLQRLLSNLLEAAPPQAEPAWREVQAQLAVPLTPSKLDPMESDSMDCDTGLGGAKAGGPKHGAAAVTSVDLCGAAIAKVLWGAAAGVAAVQAAAATHSGGVLPRDGRVLGIPAVPQEDLVMRARQLMTAVRQAAALLGSLNAVAAGAIAARSTAAPATSIPTALARQWQAHLLGCLEVTVTHMRHVGETGAAVQPLVSEVAELATEMLQLLKPVQLANNGSAPGACRGDLNSSGAEMAAAHAAVRLIARSSACMSPAALAALAPALTWLAQQGPPSVAADVAALAAYCNSPPPETLFHALLRNPHPAVLHVALQSQVHVCRTVPQQYAASVRRLFPPDFAPQAVVAVQPQPQNLLGSGAPAVHLAAVFKSYMSRKVPDEEAAAATVAAMKGRAAEGVRLAQAAREACACVSGAAPGGPATAALALREAVNHGVESMQACVSAVSSLCRPTVGSGGSCGARGIGGSITITPTEAAGDRNGAALGTLGSISLRQQVSCSATPQQQPQAQQQPQALQQFAAGPGTAGAAGSLTPLVADDNAAMKALKSALGGLERTLGALHASVVAQGINKGRPSDWDQLQVHTRALLGDLSSATSRIRNMNELLYALQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8X8</accession>
    <name>A0ABQ5S8X8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009724</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_uS2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="406" mass="44359" checksum="A63D676DF53F547C" modified="2025-10-08" version="1">MLTRLLAFGRPVAMAAEVASRATVTGYDSPLVASQIPLPVPRDGGHDASTSYVSCEKAHHAWIQQQSRMGRFPSAIRHPYATLSAAVSSPSSPASLEAAGGDAYADAAVLEGLDMESLMRILLHPLPLGRKVNPSLFSGAAAPVVQLGHEALFFDPSHTLSSTMRALHVIQRVLHDDGHVLVVNPNPAMRPLLREAAHLCLNRNVWFWYRDWQPSLLADTNRSMAAVLDPHHCQPNRRLMSAQGLALRNPLCPPGSLEHNLRHPAPKLNDADMKQLEAGRKATSRQRRDRERARDFKQAVEALAAARARQAKLLPAGVQPAVGRGGHADRLALMVALDLSYGGDAVREATERKIPTVSLLNGHSDISHVTFPVYASESHAGFQHFFLEWLLRVVNLPNPRSQKGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S969</accession>
    <name>A0ABQ5S969_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014560">
        <property type="entry name" value="UCP030333_Alba"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947">
        <property type="entry name" value="DNA/RNA-BINDING PROTEIN ALBA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31947:SF36">
        <property type="entry name" value="DNA_RNA-BINDING PROTEIN ALBA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030333">
        <property type="entry name" value="UCP030333_Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="2">
        <location>
            <begin position="14"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="128" mass="14000" checksum="E74EA6E7807138DA" modified="2025-10-08" version="1">MTTDAAPPTRQPNRVQVSTNKKPLQFYLNLSRRIMAEHGEVELSALGLAVSNMVTVAEILKKDGWAVEKSLRTGLETLESEGEQRSTSKPKMEVVLGRSSEFDRLFAKHPPPPPRGPKQPAAAEPVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9M4</accession>
    <name>A0ABQ5S9M4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051412">
        <property type="entry name" value="Formin_Homology_Diaphanous_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691:SF6">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="584"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="888"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="983"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1278"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1303"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1370"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="661"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="900"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="993"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="1047"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1431" mass="152612" checksum="87BE25D6356EB1CF" modified="2025-10-08" version="1">MRETKRRKMGYWEPFNVWWREYVAQHDKRPKVHEMKKWYEANAERVWGGTGPTWDETKKHSKGMRRIEDISDYFRQYRRGRRKRAAGCSMDSDTSDSDADEIAERASTPDSEKQPRSQAKAARKGQTAVAVARGRGRPKQETADFAGPVPSSGRPSISGSSAQFPHQSLSQQQPPFGTLALPQVANGHAHSAVTVGMPSVPREEEENLLHKEELLQMHHHHHRHQQQQQQQQQQTQQQHLLQEPQAVSQLVPSSVQCGGGEGPYPSTISALQQQHVSSSSMHLGVQQLRTASTMHLPPGPQLRTPSTTMLPSDPAPPPPPPMLLAPPPPQQQPQVMMGLLPASRGVGTYPSQPHEQHVHHHQHHHQHAQHDDPEHMRQHQQQHSDPLHPHYNPYQRDLLQEEYDDVASPPEPHPAQPLYPKIFGMPVPGLPFFGGWRSRSGTIPQNPPPGHSNQPPYTGHPVAAAAAAPPPPAHLQRAMTTPRTWSQPGAVAAPPPPPRRMGSWGMMRMLSMPRGIPSAAVAAVPPPPMGQQMQPPAGQHPQHHHQQQRLPPAQPQPAAPRPMTSHTPFAMRASTAIFNGIAPFFRGSASPPPQPSSVPSQPEPYLHPSAVLPAESYTTSTSPAAAAAAAPSYSPPHLYVKTSPPQPYVQTSPPQEHLHDQSMSPPDQNLNLAQQPLLRKGMYSQLPPSQLPQSDPPYMAVTPQVVDGHLHNSHVVQPKLQITAQPPLSSQLALQPATPPPVTAPSSVPATVLALPLLPPPPPAAAAMAAVPLQPQELVPCPLLEPFRTPERFVSLTRELEFPSEQIFGNELQLGVGTGANNGGAALQDDRAIVPDGFLPHVNLTHGSGAAAAPAAVAANIPPDVSMIMENADPEVLLDNNRPAAAETQVPLDRQAGTGTPNGGGSGSGSLIGRLFTRGSTMQRLRDLFWSWANTESDVPGQDATVAPGTDASIVAAAGLALPPPSPVPMASPAAFWARSNSNLRGSVGGSPRQGSSPDTGPNPFRQQLQRLGSMSSSWLLGKRSREASSINTRTSTPTSRKLQRGTSGVFRPLVDMMSFGRSSSNLGLCNSGVATADAAVAAPTVEPRPPPPPPPPQDLVLADIASSASAAERGALATVRTPPVAVTDGSVAATAAAAPELGQVMSTDVDGGRLLLDTLDSHTLAALDGEGQAAVFPEPLPLPPPPAGDENANAGDVSTIGDVANLAPPPAVQTQLHPPTMARIPAPMAPPPPPTSMDMTPPAAVALVPPAAVLPPASCAQVIGSSNTVLPFLATRAMPHQPQPPLPPNSSNAQGVSGPPVITTGARPTAAVVRPPPTSQQVPYGSYNHGQQPQIVQQQLHIPQEGPMVAPGTSTVWQQHSQDYMQLGCGDFHHNHHQLPQHNQVLPHDPNNQGHAPNPQEDGNSNGGMLLRLSNSVTELLKQVWGHHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9N6</accession>
    <name>A0ABQ5S9N6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07817">
        <property type="entry name" value="SRPBCC_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047137">
        <property type="entry name" value="ORF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33824">
        <property type="entry name" value="POLYKETIDE CYCLASE/DEHYDRASE AND LIPID TRANSPORT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33824:SF7">
        <property type="entry name" value="POLYKETIDE CYCLASE_DEHYDRASE AND LIPID TRANSPORT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="1">
        <location>
            <begin position="69"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="218" mass="24960" checksum="7D2E74927F04714F" modified="2025-10-08" version="1">MAVTVHARHQRILLSQRHRIPSVAANKCHPRRASGKTTFNGRGLGLLTDNGSLRWSMAQWLENKAQVDVQAPLETCWNLWEDRERIPIWMPWIKSVKVVESDPRMSRWTLATHQFGRDWEFSWLARNLPPVKNNKIHWVSERGSASLGIEIQNRGQIKFVRKSPDSCNITLIISYEVPDVLVPFANALTPLVEGIIQKDMERFREYVAAQATEKAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAY8</accession>
    <name>A0ABQ5SAY8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_010387</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF34">
        <property type="entry name" value="LYSOSOMAL AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="499" mass="52957" checksum="9DC13710027C8CEE" modified="2025-10-08" version="1">MLTFDGCDPGANPWVEKYFHDCVMDYRDAAGFALGLCSIFVWLTAQIPQFLSNIKNQSSEALSIWFLVEWFAGDTLNLLGCLIQGQQLPTTTILAAYFVLIDVIMLLQYIYYGALQARRKRLRARVSRKRHGQRHASAVHHHNRDDSQLQVASLLNDGGANRDSNEPSSVPESSTVTTPVGGSVQRIFERAGSVVAASRNPLLTLTAATAVGFSVVCMAVFALHPGAATSSKGVERHLLSVNKQQQHPAALHDGTGGDNFGVGGTSISHSSSSSDAALRLAAAGTGAALSVLGRSLASVLHPAAFGGEFLRLGHGDGDSGGGDDSVSPGGCSFLNCDVALVAGTAMGYLSTCFYLASRVSQIRKNLARRSTEGLSPVMFILTMSANLCTGVSIVLRLRSLEQVKEQLPWMCGTFGTIALDMTLFYQAITLGHEGHEHCHGHGHHHTRHGHSHCNTHQHSYGAGNGLDAGKPADLEAPLLIPVPNTEAGESSGGGPTQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAZ8</accession>
    <name>A0ABQ5SAZ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_010687</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000039">
        <property type="protein sequence ID" value="GLI66740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0157">Chromophore</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1822"/>
            <end position="1843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2033"/>
            <end position="2054"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2543"/>
            <end position="2564"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="607"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1295"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1428"/>
            <end position="1690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1742"/>
            <end position="1786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2155"/>
            <end position="2193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2220"/>
            <end position="2263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2606"/>
            <end position="2683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1300"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1428"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1450"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1487"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1592"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1603"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2164"/>
            <end position="2176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="2224"/>
            <end position="2233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2253"/>
            <end position="2263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="2665"/>
            <end position="2674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2683" mass="289580" checksum="C3F27215017663B3" modified="2025-10-08" version="1">MAHSDASSMRSANSDTSAVVEAAERLRKRHGLGAEEEEGDLLEQNKTLQEAIFSVMYMLSRSRTADSWRWAVLKIVLEGLVPLLVVFNPRHWPIDKSNPVWQVVRWAQPVTPVASLWGYDTYVRIFHALVCLIFSVVVAIGLLTVAMRKQEHSKWLLRFARVLQKFTDALFNMAYVAVFDYIMFLFNCRFGAHGHPHQFWPDVQCLSGRHVILLAIAALTAVIFYFTTALMLVASCELSPVAGGIMASPAAVTRLQVLMLKALFVVAADTMVAVGKIQAVVMLAMAMGICLLNLKALPFLRLYINDIWVGIWVAVTYMCTVHMAMDLQDDRQAMLLTRVVLYGIIPAILLGAGFTACWSRWRLRPAAKFRGLDPTTARQRRLYTFKSPSDVEVVARAMRHFDSDGVILEDAGQLGETIIKCGITAFPGDVGLLILYANYLMEVKRDGPAARTQLQLALKGGPSLVQRYQIFSTIENSKRLKDGQEGQLDLQSYVEFKRNYRAVIRMHKAALAAQRDIWQLLQHPAVRASAMQAALNALDAAAEAAQRVYRRVLERYPNNGKLLRCYGKFLEDVRNEPEAANRVYADAARNGGSDGLLSLDLKIQGADKPEFLTSMDLHEDACVIINAEGKILMVNACVSDLLGYPRAELEGANVSMLMPQPFSSRHPGYISRYVQDGSPHILDSVRDMVALHKDRYVFPVQLCVTKLSGVGTDAVFLGLLRPAPFDTRHVRAWVTPNGVILCTDPQFASLTGILGEEMVGRNLRTLSTDIAAVDSLLDVCRTAPYEKLVAGEISINLEIAHRYLPPLPVHLTVRPGGTDAQRIFIINAVRRTCGADGGSGSGAAADYLSSLPASSSMSSCSEGLMVVDARGALVFATWDVAAMLGYPLRSFLRMKLDQLLAEPFATLHTAKYLRDTPVTIPPVSCRAGRLVHLVNSSGANVPVRLKISTKEDETGTAGRNGVGGRTQHIIQVLKVDATNATALYSDKRLVLLCSMDGRVLSVDQPTSSLFGFTASAVVGSNVADCIDVFAEWRTKAGAHQLELLLLSLLDKEAEMPGTSWRVRVHSPEAEDSVFVSANDPKIPSRRPAGRSACLQTELMETYEEDATRGVVGDSTIETPARAAAGGDGGAIKIMARIILWRRDVLTGVVELDDQLIVRRADVSTGLIVGLPPPSLQRMPLHRLLDINKGMSWQDLMLSDSRKHKPSALKGSTTAVVSEPRAFIGPHPDGGTMRIIVQGVSFLTGGRITATLHPDTTYVGARANLYRALGLEALIQHQQQAHQQQLQRQQTQKVVVEEPKQQQQPLRRLQPTETLQLRRPASRAVLPRSDSARNCDSRHSWIKTDHNSARYDHGGVAARTGPGRTAASGLVTETVTAAGPTVGVNVSKESGKQHEMAAGDRDDLKVVGGINNGRGGREMIMESSETPGLVNLNGAAGENGAGRDIKACTGTEGGDSGSDRSGGGGGGGDGDGGGGGDDDQSRGNNVDMDIDVDRVDEEEDGSNDDIDEDDKDGKDEEEEDEEEEDEEEEGANLHKRAHSQSDFIASWVRSLSRQMTAARDRRNPSVVPSKEPSRKTLNTESVTIPPVVTADASRPRSRAEGGDRGGASRGGGGDSSDDHTRTLLVRASTKLLARGLGAPPATSFCRRKMDPPADPLANDPVLGWDDEDDHLLDSPGGRRTAAEDEDDSERKVNRVVHPLEAAGGGGGIMGSCGSDDGADGNHRMSGAHCLGASQEATDAGGVRSGDGLTAAVEAGGGGGSSIGDDEEGSQGHGGRGTLSDVTSGTEAEITTDARRARLLKRTTKVLSGSTLELSLSRFLRRTLLLLTGMLATHVICYIVLMGFVDSQYSHVREIHRVALAADRCQISALKVAVAEFCSRPGVDPVSVCEVPLTMTLRDLRAALDDLESYHHSVYFGTTTESGKGEGQTPRKMNQGALYDMWTNPVVAYQLYIDVDHPRWLNISAGVWQLGNRFLATGRELLYWGQRLAGNISDLRSFQFLQANGPWSLFHAYTVSLDYLASMAWEDVATLQNSLLIILLIEVVAVQLSCILYEWWLVRRVELRRCGTLLVPLGLPAPVLKLLSTRPLVVVEDSDDEDADTEGGGMGCEVSACGDATAPSHGSLDGPSDAVAPFQGHAVRTTRQRAAIDFAPLRADQDLDNGIGTDNGAADSGAAQDQDYPHGSAIRTTHHRNTRASIDFAPLRMTAEGAGGGVGATLQVASKARPSPPPLPPPASQQSPAGGLSDGKAPPPLEQKPESADDFDSERAAPVRHGVMMLGVIDDGSVNSSAAVASIAAASADRGGGRRGLAVVVSEGRGSRLVVNGKILSANSRSLLGFLVPLLVWEAVLAAIDALSYFWLEGMQGPLAALNMASHVIYRYTRVRMLALFLVSGKDAVVRSHWRNNLSSELANLRNEYDTLLYGGVASTQEGSVFQKPVPASTFESSSFAENFFRQERCFRWDQSQCYTPDSPYYEMTHHGLDAMMRRVISELELLVADDDAGANYTGSRYMTMYMVGTKDLYEGLQSSAQLFVDFMISRYQNVKLLHTLLLVGTILLFLVYGLFILRPYLAAVAREAQRLAGLLSHVPGELDVVSYTRQVMRAAAHAMGTDAGGGRRDGGSGRRQPNVKGAAGVPTAAPFVSASSGGNGGGTYPHRRSNWKSRGGAAAFGGKGASTGEGVAVSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB04</accession>
    <name>A0ABQ5SB04_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_010716</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13990">
        <property type="entry name" value="STKc_TLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF23">
        <property type="entry name" value="TOUSLED-LIKE KINASE, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="793"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="406"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="493"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="406"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="417"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="444"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1108" mass="114308" checksum="BA7A96781B940280" modified="2025-10-08" version="1">MPTTEPNPQPASATGISSIITSISSNEAKLQALERRLNGGDPTRVTSGVPGSGPIFVGTFSPLTVTLPHEAPAPGSAPSALGVVTTANTDVTAGQRLPLVAATAGGGAMALPGPGLGRTFSEGRPEITSTTAAPTTSISIAHTATVAIASGDMTTKRRRADGVSQGSLPAAAPASPSNSRSPLSGVNSFGRAVHAPLPLLSAVLTDGGSAGGAVLTQLQQQATASLASATAAGATVTGGSNNSRTRKRKQQDPKHEAMAVVAAATPARVAPAVLTLLPGGGTAAAEGALGAGQGLAGGGTGAAAVLTPRRARGSAGPGQERAGNPGHQNAATPGLARHLFEETGPVPGAVGGLCGRSSSAAAGDGGNGRTAAAVVAATAPTAPPSAQKRSILSYYPPVMCAAAAGAGAGGGSGGSQGLTAAATPPLLLPDQNSRDSLPLSIIRQQQQQQQQQQQQRPDMAAPTNPGAGLGGAEVSAGGDDGTSVSADTSQLHLRQSQQQITQLQAQVMQVQAAMAAQQAAAGAEIERLRAELRARSLEAASLQERSRAHEGAMQQAVAGEAAARAALEALQAASAAEAARRAAETAETARLVSEREGRYRGELARLVQSFARAERELSRTQQSGNAARLGSLTLVRTGPMQMAEVWEEGTAFQQLAARRAAIAAAKEEVVAAQKLLRKRLAPPPSGARGGGGASCAATNVGIGVGDESNSCAVYDIMPYLSGLEFVAADEVYKTRLAVLKREEESIREEEERLNREKFKQIRAVKLAREEDASRFGRWPVLPDKPGPEGRPRYVLMNLLGKGGFSEVFKAYDLAEARPVCVKIHALASGWSSAKKESYVRHAIREYDIHRSLRHPAVVALLDVLEIDLDTFATVLELCEGGDLDGLLREHRTLPEREARCLMCQIFSGLRYLASSRIIHYDLKPANILFDSLGQAKITDFGLSKQVSEGQTLAGIELTSQGAGTYWYLPPEAFEIGGPTPPRISNKLDVWSAGVIFFSMLYGKKPYGEAMSQEQMLRERVMAMPREVDFPSRPAVSSEAKDFIRRCLAWQQDSRPDVEAAATDPYLTGSTTATVASSSSPAPATGGGGALSMGLAAVVSSGGGGGGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBI1</accession>
    <name>A0ABQ5SBI1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="444"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="460" mass="47809" checksum="AE2FDCC47598D793" modified="2025-10-08" version="1" fragment="single">MHGIRLDSDVEDTPDKRERPQIQHTGGQTFRTNRGSTENDTAQEKTISPGKQLLAVKAVRRSRLRLASKGSASALAPLSGVPHQPPGAVPSLRLTAKEDEASSQQLPSRAQQELAVTPQRPRSLPEWPQRISAKPKAAGASSPHVSDKDRDFPPRASGPQTESPGQHQSKDAPPPPAQAAHDAADDAPPPLPVPTVSADERALGGGVGAAAAAPKKSVHAKLNFFAPHVRRVSKPVMVKVDSDPGIVQIPAAAQPALPPDSVRVHPCNPPQQQQQQQQQQQEEVMTVKEDNEKDLSGSVPQPQEAPAVVALVSEAPRSHDAAQGASAPQNEKGPEPEGVPLTEYVRLSIDAEGTAAEHTAVGGAASTANGTADDAHCAEAAGGSGGTGGGADADACGFPQDTIAQQQQQQPPQPYSGLNGFESWQDHATTGVARRPASRTCRQALLPGALPPSAAAGKVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBW5</accession>
    <name>A0ABQ5SBW5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010614</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051412">
        <property type="entry name" value="Formin_Homology_Diaphanous_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691:SF6">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045316104" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="46"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="404"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="554" mass="59618" checksum="049D789E9040158C" modified="2025-10-08" version="1">MRRLPPALWLPGCLVCFLVVSSWTSFAEAQLDFDTEDPQAGTDTRFPYCVCDRPPLNPSPYSFLPIVEVKGPGQFCFTLRVNTAGCTFGKCCNVDLKKIEFDINGKCLVKSASIKATVNGQFTTTGPNIYVYKFDSDLKYVLRLTNLNMGLNYNNAKICLSLTAGTNDTGCDTLEELCASPSSSSPPGTCQASIFNNNNSTGSCCPTTIVFMPRPPPPPPSPRPPPPPSPSPPPPSPPPPRPPSPPPPSPSPPPPPPPPPPPPPFAVNKCKQCTIISLEPTAPFPPISLSSDVCNKFVNILAEKLNAIVSSLDLTMERRFSLDTSECQERSLKVCGEFSDNATVATNRPLSQNFKEAVEGVLADLAVEFCKYGTGMSVYSVDDSLNCFEVGALPNSCSPPESNPTCKCNQTAGITPYYALSTVTEEPGRRSGRQLYCFTIAEVPSDYYNPTTLCGPSLVLSKVEIFANQTNRHSVTGIRVTPGTGPVLWKATSWADNTLKVTNLRWDKSAAVGGTICIELSIPLSELCIKGATCYLNLFNENSKCCPSYGVPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBZ8</accession>
    <name>A0ABQ5SBZ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011635</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXA1/ALB3/YidC (TC 2.A.9.2) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF65">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="9">
        <location>
            <begin position="161"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="399"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="423"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="443" mass="47924" checksum="577A7E21547AB746" modified="2025-10-08" version="1">MTQRWRSFQRGAALLLQHAEPSTFGMQGFQSTYLSASCLIHHLQSDDQQRRGGASGQQKYAWNGSIGVRYASTKSSSGPASTASSGVADDIPTTSGTDLMPQPEFFPLPESSATEVATAIHQAFDALERASILSAKADAFFAASWCISGLEVIHDILGSPWWVSIMIFNISLRLCTFPMMVLAQRGSAKMMEFNYALLHAKKLQEAAMKATNRAEHDRLFNAFRAEYTAQVSKHGDPVKTALMVPGVMIFNGFVFLSIFNGISKLMAAKVPSLTTGGAFWFGDLTQPDPYFGLPIMCTLVTLAMVEYGINLAGDAGPMPTERQQATKTMKWIFRLLALMFIPAGNYVAAGTALLWVSNTAFGVTQGMLLRNEAVRHRLGLPSMQALRDMNARVLEANSANAPKPADPPASLFSTKPSLDPSPAVNTPLMSNPRTAGGQGRSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC46</accession>
    <name>A0ABQ5SC46_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_011105</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1060">
        <property type="entry name" value="NPCBM/NEW2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052970">
        <property type="entry name" value="Inner_ear_hair_cell_LOXHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038637">
        <property type="entry name" value="NPCBM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901:SF3">
        <property type="entry name" value="LIPOXYGENASE HOMOLOGY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901">
        <property type="entry name" value="PROTEIN CBG12474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="359"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="448"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="1445"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="1723"/>
            <end position="1839"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="2327"/>
            <end position="2353"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="2436"/>
            <end position="2471"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="2674"/>
            <end position="2709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="703"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="785"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="720"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="794"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="838"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI67009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2747" mass="291925" checksum="4CC500C21CC25247" modified="2025-10-08" version="1">MQQAYEVRFHTGDSWGRGCTSLVAFQVYGSRYILSSESCIQQELPAVSAKRSRAVQFAVGKADSAARKDRGDAAHEAASPPPEKRNVFALLSHWLKTSGGAVQTKQLVKVVDEGGDGLLGMEEVKQLLSQAVPSVSDLETQLFIAMVDANSDGVLTERELLQSVGDCAAIDVAVASGGSGPGPVQDPGGHAEDASSPGAEMAAALAAAVQHLRSNAGAVDSKLLQMSPPGGYLTYGQLNELLQAVVPESLGGRATVRALMAHVARTANVSALDPGVHVLEIKRCFGITAATVRTAASAAVAAGWVPGRPRNIFKLLRYHVKHNRAQLDSLFPRGLELTANQVRHVVRQLLDTRTGEPPLTGREARHLVAALDVNANGTLSREEFDKGLRECRDISAAMTTVYGRGLDPAGLQLDAPLYPPLTGEGQVAPPTWGSFLEALRQLHQLLMDRSEEVEAAWRLNDSRGKGRIDACELRRVLLDLAPDYLRAVLIYLDGWDPSEHGLLSRKHLLTALRGDRPPDSLQPQPVVPLVGAGEGVTATTPPLQGISQGITRALYGGNVDTGLRGLLPDPAQWQEWHGNVKTPGRPSGSVTLGGGGGQLPPRSGFWKAQWKWHTAWLAANEAYRQAATGLPAAGLGDEKEPSAAAAVAPLSSSTTDTAAAWASGPPSNCLECRLWPPMSVAALPYISAVYGNELTAWKRAVGDGPERQGAKGPGSKPAAPGAPFPGPAPPGSGGRRVRGAVAEAVEGDGEGAQRLEDASTRGSASTVPAAQIASQVLQDEQSVLEGKHLPNPGGPAEMAGESAAAAAAEASVPRENAPEANVSTAAAFAAPSEDVSAPEQSVTSPQQAATPVEGIERPAAVGAGEGPPSSQREPLLHRLNDATASRQLVAPEPADVLGPEAGRLPESHTPAAVAAAAELPMTAAAVPDPAKGPLPEAVTTVPDRTSEAVALSSVAMPVATLTQSDTSGLPAGSSSRALPVADDGGLVTPAAAPRLIRSSPQNPSAAQPPQLFQAPPGLVPARVLSYKAILAAGAQLGNVESISVKASSSGPAWYLDKVEVLCPTCDPGLLWEVTVRRWLQPGEQLTGLRPDPFAWEEWDMQAGDSPGAPVLFFNRNLGEVSWTAPPRTPLPALPGAVPNLRLTGDMRMSPSSSGQQSGEWLGGVTPIAVPRNNVRFLDSLRDPPLFRPTRVRATYDLGFDGQLGIVPGQEVRVRGDRCSHSIAVMARGGFKVVDPDDPSAARLLPVGGASTGTTYGKSGGGSTGRPDAAAGSAVDSLGGAVFPGIRECVVLSYALDAAADALGEKYMWLDGWVALDDSAGTPPAPVRFSVVKDGALVWAKWVRAAGEVHRCCVPVVGCMEMHLVVESDFSGGARAVWLDPLLMCGPLHDKALIASLELSARANEVADRSGGDIKVSFVTEDGGAIMYMAPAQSVPFEVSRSSSTASYRISVFTGPGKNGGTRGKVYIRLQGWRGSAANGKAIKSNLMCINPDGIALKEGDKYSLLEPVTLPWMDEVESVVLQHVPAPSGILGGSSTVGGPNDWNIQHVELSCDETGRTWYFVAGGWFLELAGRQAEERGGPRVAGAQLELMLPRAASPNQLKVVFHIISSGKGVGMGIDGDLEMVLVGVDASGYQYASLSLPGRSIDSPHETRLLESPLPLGDLQTVELSCKQQGLLRTRLQFIEVVRLVDAKHFLFLTPSLDPGEPPDAATTRLVLRAATQPYFRISTFTSAIPEASTDARIYIDLFGSRGKLLDLYLRDATGDSFSEGCEDVFFFPDPGLGDLGSVCISHDDSGESPNWHLDRVEIANTVTGRTNVFVCKQWIGLGTNQQDKNQLEGRFGPDRVLERVLYPTHVLTALKDGTPLLRYHVIFHTNNARGELCNTAEALQLVGRQADGVFQYSDLVILDRGRSDMDLELDMQYVRHIQAASRAEAQRGLEDRDSSLPATAVVGNSANTCRTIFQVVIKKLALFQARANIVLQGHLGARQLALVLHGSCDSSRSIILGADNCMSTGMLPFTAQQVDDVFQVSCDTQLGAGLLRVDVQLPSSSHTPPDWGILLESVTIRELSDARGSPTEFFAGSRGQWAGASPDTPEGVVGGTQALHLLPAGCCAASFLGRALQELVAVHLRHDSPAPQSDWDLNYMEVLVSPPSGSPIRGDAGMPGTASMTPQKNGQQSSIMISPCLYHFPGSPQQPCACWPSREAPFIRQFLIDKYLLQPFPPQAYFKAPLIASPQWHESPEPAPLPEVQEGSGAGPAPIDAVAMAALKFTQKPDPERLQALEEEYRAEVAAYLQSLSGPGAAGTDVFTLMRARIRAHPGLLPREFNELDKDKDGKLSPSQVHDLIPHLLPGEVHCSEQLSKYIAAMLTLGSGEMMAQAHIVSALKACRSAYKEAASMVHMLEEGSVDWYQVAQEDGLGVEMALCRLSEQLVQPTNVQAAQEAFQLYDTDGSGYLDIGELCKALQSIPDLRVSSREIRLLLAYLFHFGDKDKDLRLSVLELQTSLAPFVPRPPAEELELAYTTYKLLHNLPALLRVVNKLQPALFSAACAQAKAAAVTAGSSPNEALMKDVDSVLLSLLPDIIAVVASGLEVPPNEVTQLQQLISLDSGNQSLSVMDLIKDIALSADAIKRAMSIARLVSNALEEHEPLALDHDLQSAEVVLHKLAQILVQDDGNKKLAESFHSLDADGSGYLEGPEICAAIRQIWESISKEELRTLLAYIQRYADQDTNGRVSMEELQVLLRPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC47</accession>
    <name>A0ABQ5SC47_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000048">
        <property type="protein sequence ID" value="GLI66907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="100" mass="10527" checksum="F2FF95CDDADA0719" modified="2025-10-08" version="1" fragment="single">LAAATETGVRPDQLATLIAQTTEIRTQLGNYLLSLLNLLKSTVAVNTNMDNTAGPRVLPLPQARGHDDIPEDPLGEAVALDQDLGATRPTPTAATRRSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC98</accession>
    <name>A0ABQ5SC98_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_011599</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046509">
        <property type="entry name" value="DUF6687"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20392">
        <property type="entry name" value="DUF6687"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="7">
        <location>
            <begin position="500"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="597"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="609"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="706" mass="77249" checksum="49D66392BF42982C" modified="2025-10-08" version="1">MRVATVLPRPAGFMAATGVKLPAVAVLSIQVCLALCVLAALAKLLLYATRLALRKTSFNYVPFSHDLKDVLVTDCTHTHARTLTHHKVQRNPTRLRPSDTSTGLVLNALKATAWSAGQAYEWCQLPQVSTNHFDVDSFLAVWCYINRSLALQHEEVLRHMARIGDFREAFLSPQLVATHGSEDGITNIRDAFTALKLVCWINSVERIRFSAPYEERDCDAKFSFFLPRFADVLSNPEAVWQEWQDEYREVVSGFDVLTGEPPAVERYSAVGLVVLRPPEPTLHYYSLFSHTIGYDTVLTMYDSQRYELESKYTQFISCRSRPVWPRVDMGPLARLLNRVEGDELGPHLRWACARFTDTGPVLRIENTAAPLSKAQRYGHPYARHLYCSTLPPATLVAIVVSYLEYGMQGLKPKRGGWTWDELQALNNGISWQEWEDAVWRQWERGELDLDASTGVLDGAAAASRRQGSLSGGGAGALPRVGIAMTPGGGFWEPTLSEPSAVMPLDDLRALAGAVPARLATRRWQLLYGSARDGISLRTLYRKAGGRAPTVLVVREAGACGHVFGAFAAEAWKSGPRFYGTGETFVFMLQPHRAKYPWQRPRHGGGGGSATAAAAAAASGEARDRSGGGSAAASASAAMAAATPGGSLDFFQFSTHEGLGVGGCGTFALWLDNELLEGASYPCDTFASPQLSSKEEFKVAAVELWQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCY4</accession>
    <name>A0ABQ5SCY4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein 208</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_012084</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM208 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008506">
        <property type="entry name" value="SND2/TMEM208"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05620">
        <property type="entry name" value="TMEM208_SND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="20076" checksum="B760ECCE6EBBECE8" modified="2025-10-08" version="1">MTNQGAKKRLEENRKRLQVLRIAMAVGTALHVVVRLVLRQGYDSSWHIIGFGSTFFLELFSYSAISKFAEPEFNEKGDIVFGGSDLNMGGMCAYWHDLLYISIFVQVTTCLSTYFWFTLLVIPGFFLYLLYKNILQPYWAQSRSQPVLDEATRKRLERSQQRAERRRMKWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD71</accession>
    <name>A0ABQ5SD71_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="218" mass="23757" checksum="6264B37A8CBC3595" modified="2025-10-08" version="1" fragment="single">QVAVGMAYLHSQSPAIVHFDLKPENVLIQGEGENMVAKIADFGLSKRKHGKYVSCPTPRGTLPYMAPEVFFGNGEVSEKVDVYSMGVTLWQLHTGEEPFMRLSNHEILMGLESGTLHLPIPSTCDPEWRSLVETCMDPNPDNRPSFREIILQLQAILVQEQWTAKKSCSCAEVAVAAAVAVLDSDTIGRTLPRLYEVLLSPATPAAPAAPAAPPLPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDW3</accession>
    <name>A0ABQ5SDW3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">alpha,alpha-trehalose-phosphate synthase (UDP-forming)</fullName>
            <ecNumber evidence="1">2.4.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012454</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-trehalose 6-phosphate + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="2.4.1.15"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012766">
        <property type="entry name" value="Trehalose_OtsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02400">
        <property type="entry name" value="trehalose_OtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF106">
        <property type="entry name" value="BCDNA.GH08860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="654"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="910"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1013"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1304"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1484"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="656"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="916"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="941"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1016"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1034"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1356"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1379"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1392"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1484"/>
            <end position="1499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1547" mass="163116" checksum="75814301841F9A7C" modified="2025-10-08" version="1">MSSLPEIESEELPSSLPTTRVDRLLRERIRRNRSHQAFDIAEDMPPEDPVLPAVSEPPIQARLVVVANRLPVTCSRDAQGKWHLQASAGGLVSALKGVSNYATLWIGWPGIYVKPGRDRDDLTAILEAEGFLPVWMDTTLLDLYYNGFCNSVLWQLFHYVPLNIDTWQKMAEHRAMQLQWQAYQASQMANEKFADVVLEAYEVADVVWVQDYHLMLLPSLLKQNVPKMKVGFFLHTPFPSSEMYRTLPVREEVLRAVLAADLVGFHTYDYARHFISSCTRILGLEGTPEGVENNGVLTRVGTFPIGIDPERFTRALESEEVQSQTAKLLNRYAGRKIMLGVDRLDMVKGIPQKLLAYEKFLEEHPEWRDKVLLVQIAVPSRTDVPEYQKLRSMVHEIVGRINGQYGTLTYVPIYHLDTSLSFTELCALYAVTDVAMVTSLRDGMNLVSYEYVACQSDNAGVLVLSEFAGAAQSLGAGALLVNPWNISDMSQAIYDALSMSEEERRERHRQNYMHVQTHTAQHWADTFITELNDTHIEADLRMRHTPPPLDVQEVVRAYQCSKRRLVVLGYNATLTTSVEAPRLPKRQFEQVKALAKVNPRVMQSVCELCTDPANVVVIFSGSETTKLDEIFGHLPVWLAAENGVYVRPPAANGNAGSAGAGRQGGSGGPGCSQAQAPAEPSASPQWKCVFDQVHCEWMESVQLVFDYFCERTPRSFVEARETSLVWNYKYADMEFGRIQARDLLQHLWTGPISNAPVEIIQGGKSVEVRPVGVTKGLAMQRLVGLIAAEGGIEAAAFDMVLCIGHLLGRDENLFSLFEGARIDGAAPVHNSRSERFAAANAAAAAAAAAGQTGQDVLLPATVRTRVGAVGLGTLGPPQAVHHVRSSSTGGLQGTVEELQSGRLAGSSGTAAAAAAGGSGSGSGASGGAAGSSSAAAAGWQPPSQQQLQNPKASSGITQALSRTQASSPFLAQSATPLPQSNSPRDQTLVPMAAAGSSAASLLAPQAAGAVMSGSTAAAPAGPPALAYTVPPTRSHGHLHAQHHAHGAHPNRAGLGVVPGHHHGPGAQTAVLPHAGILSRSASGGGAAPGGSAAATQTGAVAGVADSAGGPGGGAVLPGAGGSMPAVIRKGPGTGAFGAASLAGSSGHSHHGHRPLQTHSMDRLDMTVRVVAAAGAAAASGNSTVADRLAGIFPPKYLYTCTVGRNRSKARYALATSAEVGDLLDAIVQQGIYHRPRAAACASSAPAEFSLGGLGLQGARLESVSSADALSDSMLTSLVGAQAAGMAVAGMRGFREGDRLAGMSLPLDESCSMPQSPSDGCATAAGPGVSSRGGTGPSNAPPHLASLGHLPSSQHYSFHHHHHGVHGHNHHHNHHHNHRQAQPQTRQAQSSGQQPMAQSPLQQQQQSFGASDLWNNFESRLSVSNVGFPGDHLGGGATDATDAAGSFGSGSEHPFISASIGPSSLRHQPMLGSQLLLGAADAQLLQRQSQQQTGGPQSGTMPSPFKASDALGALGPAGRADEAQAAAQGPERGQAAGAVQEASSGHVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDZ6</accession>
    <name>A0ABQ5SDZ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011834</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="156" mass="16766" checksum="DEAA44FC03458708" modified="2025-10-08" version="1" fragment="single">DCYMVAGGLVAYDEDGYKSVISGTEDPLHAVRTLEFAKAMIRVSREVHMPHTGEPVQLRVGLHSGPVTSGVVGDTMPRFCLFGDTVNVASRMESTCNPGHIHVSAATHSRLPSEAWRDLGMASVKGKGAMHTYEWAGDVDGPLDGEQVARVLGLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEG5</accession>
    <name>A0ABQ5SEG5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Domain of unknown function at the cortex 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013897">
        <property type="entry name" value="Duc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826:SF2">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08588">
        <property type="entry name" value="Duc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Domain of unknown function at the cortex 1" evidence="2">
        <location>
            <begin position="273"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="568" mass="61608" checksum="4AEBA2C850861032" modified="2025-10-08" version="1">MMIALLFRGLRVSRDASLGPEGARTTAFHQEQRQKRQRKHGDVYEELYSLVDNSKVQESYEELHHIAASTSSTWAGRTPASVQADPRSNWEGTAPTSELVAFPVSRMNTGHGNDDTSYITTSTRPPVSGVIAASQASSHNLNDSLCTSTRIDSSSSSSSSMSMSSGKGQGLCRGSACACYCCCHCDQADNECRSGDDVITLNIHSEMYCCASGGGGIGGDESSCLGSPYDCPAVLEALCNSKDFPVLLLPGAPYPHHLAARYGPRSPFGFSPGLPLNSPHRPISVDTHIFIGCAYVFLPGLSSTPPGLFKGRKRRFVLVLQGRFKRAVSAADLMVGCHYRRPLKLEGSAVPVASVLSWVSRLLAPGGRGGGMELVLTGRDPRVRAPLAAAAQMIHVARPGEQPHPLKAREDTRLMTPPLMDSGQGEPWASARRRHYFRQAEHRTAHVFDTEHVWTFHTWDQALGYGECSDAHTWPPPSTQTSCRSGHLRRSGAILGWPATANPGGRCPQWSSGAPCGCMQLETGRCRDLSHRDCHCQLRHWHCRLHRHQPCLLTSTIPPLDRLASAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEI9</accession>
    <name>A0ABQ5SEI9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plastid lipid-associated protein/fibrillin conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="26995" checksum="0D6408E0884C82D7" modified="2025-10-08" version="1">MTVTIRLETQKLSCKTPKVAPTFVTARCILTPQQKRVAQVRTYSYQDYGSTKGLSATTPATGRSKVLQLLGEGLNMYDGERFGPAVAQLVAENPTLQPGNQSVELGQGTWQVFYAPHIFKLSSAMTTKFQPIQYRLSGNSLVANVKYSNSLLGEGWLSAGGIMARKYDDAVEVTFDRFWMDMGADSLRADLPPDASSSLLTADGIIGALGRASFFPQLAVFPVLYLDSDLAVFKFTPLDSPIAVHRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEN8</accession>
    <name>A0ABQ5SEN8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_012095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAMOX/DASOX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006181">
        <property type="entry name" value="D-amino_acid_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023209">
        <property type="entry name" value="DAO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530">
        <property type="entry name" value="D-AMINO ACID OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530:SF11">
        <property type="entry name" value="D-ASPARTATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00677">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="39"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="300" mass="32411" checksum="AC9FFF739237DA5F" modified="2025-10-08" version="1" fragment="single">HSGLADVAGVSMVSTNSLFPEPQELPFWSHIPLNFALMDERTLAGLSQRAMSGLEGIQATMLPGSDCVVPDLDPNPFQCGWGYHWQSIVCEGSRYLPWLTEQVCALGGRLRRVSRLTSLEQLSCPEGDLREVDVDLVVNAAGLGSLELLPDPEVVPIRGQVVRVEAPWVKQAYFYEPYYIIPNRDTVVLGGTGQKGDFNLAVSQDDKRRILDGCCRLLPSLRGARVMADWVGLRPGRTSLRLELQREGVRLGGGSSGSPGGGGRVVPVVHNYGHGGAGLTLAWGCAGDVVRLIVEEGLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFI4</accession>
    <name>A0ABQ5SFI4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012557</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="125" mass="13679" checksum="5A8F9021E63EE718" modified="2025-10-08" version="1">MELELCRQVHFSRSRLQSDALRPQSCGVAAGAPWRGQVWPSFYHAFIATVLVIHVLPTHQHNIKVQKHPVSISRGLCGFQLLQPPFPAILGPAGIVNGEIVDSRHHCSGGNNCQAPRGRATEVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFS3</accession>
    <name>A0ABQ5SFS3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="613"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="898" mass="94153" checksum="9AC42C3E1FA04A80" modified="2025-10-08" version="1" fragment="single">MAVRSIARQLTSLGIAVPGPSARLKSVRKRQSSIASRAAVEATVPAEVDDAAQTETPTRKNAPAIEDAIVRNHGQPRRTNQWNKGKRAEGGSDGRDSSSLRPEAFDRAIISERSRLLLNGEAVGLTMKSTPADLAAGFKGLRPLAKELRRKNQTLLFFPSTRATKPKQSDEPSAESANNSEPQQQERKPATARELDLALVAVPYFPKPEAEVTIRVGARSKSDVIARTLTGALLRSSSVAVRAAGPAAALVALEAAAKARRAIGAEQQLDLAFSIEYLRVPAIEAIKLPGEDGDVVAVAVAAAESAAAAAAAAATGAANADVPADIFPGAQGETAASSTAASSTAASSTVASSSSPEGSAEVTSILQFSFYKRVLPTPEVLASDFAATRSGPAMAQLLIRLISERREVDLLLDSRDALFVMQTLARVVRDSWSQSKASVGVFLTSLEYQTEEDSDSESAGAVSENPGNDVRFTARLQLRRILGAPNLVSVKDRRTLLGVPVGTVKWDSPIGALGGAMVRELYTAGIFSVWPQEGICVARMLRALWKAQQTRAATATATAAGSGEAAAVTSVADPTGSTDSDDTAAAAGAPSSSLIQIALVPELQEEGSGSVEARNDRGDRRRTTQREDGTSSRQQVARSTVLHLLEVSPALTSTLRSKDGRVVLISGGEQLHQLIKAVVLRNRMYGSRPGGKLLTLRPLLDDSGALAVRFHYIDNSPIAEIPTPPGAGRELIQPVVRPRRQAAALPPAIEDGYATDDEFGVGAEAAVLVPVAAAGAAAAAAAAAGGSGSAAGATSKGVKSPWIMFATSNTSSTKLAMGIVAETCKPYDRLCPIWLKVRVLPDTLATAALSVAMSQAQVQERANVELLVLPVLERKRDDQNRPYSTLTWQLIKFGPGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGZ9</accession>
    <name>A0ABQ5SGZ9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="498" mass="51255" checksum="81AB9DB6B9241FF3" modified="2025-10-08" version="1">MANATEVNRTSGTALPGLEHLRDSVHNWEASVRTLSLTAAAMAESVGAGMAAQTLASYGQQPERTLKPDLPPVLSPFPPFPSAAPVAPAAALPGASSSLLASSPIPTLVVSGSQLKAGRVFIVANLQGAYYTLLEFLSRQKFDFRSDNLLHLGNLVAPPTEAVATVAASGFGGGSKGSPETPGPSRRRRNSTSVLKLVRRHGGLGPMGGHECLALLVTARANRMLAFNQAEEERRLAALLSQKQQQEAEAGAMVGFKKPSSSLNVRCSALSLQLAGVTATGAGAPTPPAWCTSAATPVNNWLGPDCSSQSDSSDGASASDDEMDVDVVAASWTPHRDGSMLSPSAPATATAGCSTMMLPGGAASGLSLDKLDLSQMLSMPACVVLEPYGVRLQHAGPTDAADVAPDAATAGNRRYHTLFSHRRAGGGATVAAAGGGVNAHFTGISSSRDVVRFAVLPPLRTLQRLSPGFGAKMAALVAPSREDLMLEILEEPVSELDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH03</accession>
    <name>A0ABQ5SH03_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013786</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046770832" description="Guanylate cyclase domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="1982"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="662"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="4">
        <location>
            <begin position="707"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="913"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="962"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1067"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1200"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1295"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="438"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="913"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="931"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1210"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1301"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1341"/>
            <end position="1357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1982" mass="208566" checksum="3AD57E2B270DFD3A" modified="2025-10-08" version="1">MGTYAAYLAGGLILVTLVFASTSQALDGCNETKYVCISDFLKNVMQAFSTSNISIQDPSATYNALNTAYGNFSERCLGISNGVRRAVRLLTPTIGDNSQVQLEILSQGFTRATGYRLEYVLQTPARIEEEVQFQLQTNVSVYDGWLLDVPSSEAARRASGVAKLDALVAAAGQQELQDSDVHPFLSKYGAMYGEHRMAVVLSGFVPLLYWRRDVFAVAKLMAPPTTWEELLAVARLLNGTDMNGDGKPDAALCLQMDECIDASTILSNILASMTQYEGPSSGWLFRPEDLSPLASGPPLERTLELFRDLMRLDARGTKGCSFAHQAFFNGTCAMTIKLAQHFKIAELKHPELRGLVGTAPLPGSPTVWNRASNSWLTCGSETSLTSPCPYATWETRLVGLNQTTIATTRARSRSFLPVRPSVSIHTTSSPERLRGPVLGDDGSTGSSKSISGSLGRRTMSVVASQSPPPQLTAANDAAPQRMLVNRSPFLGMTALLGSIDARTPPDYAAAALSFFALVTSPEVSWAQFGTDSAVGPFRRQHLDDANVWRWLAFNYSREDLTAFLESTRQTLDHPNAALPLRLPGAATLRDVLYEAAQQILKDPNVSVAALATETRKELVAALDVGLPMEEVQRIYWETIGFKGTKPPPYSDESWFDSRSARIGVGLAVAVAGSMLLALAAVLWYRSRRVRRTLFGRALAPGSSAATSLVVTDIVDSTRLWETVSPAAMTRAVQLHHETLRSLLQLHKGYESATEGDAFILSFFSAADATAFALRAQAALLYQPWPDELFQHEPCRPQYALCQAPATDRPRAGITRLPRGAAHGLPSASNAGPEDSSNRVASGAGAGGIEEHFTSKWLAGSTSQLPLPSAKLKMMTTALSLPLKRRQRVLPGAGSGGLPTAATAVAALGPQNHSQPLLRQRGSSSSQLSFPHLPPAPPPVQPRDGFASHSLSQLLLNGSVRNGITGVGGGEPASTEVSTGRLGGGSSNRALQLRTASLRDSHKLRQLQLAGSRGRNISGSVGPYVSRTLTNVNALQGGDGALEGGPVTSTSSRVLTAGGAAPWLLIRDGNPSGATPAAPREAQQQRSGMVGDRSDDGSGTVVYSDTRSGVRPIERDVCQLHEQVMGLGSYGLDAGTRITKAYTAPVMAAATEAAAAADPLSAMAIAAEIAVAATQSSAVTEGYVWDVSSYEPDVGSIGFGMGDHGTRKNGEGGGGTGSCSGGGSDNRGGDGDGAAGVATAVAAVSSRLMDPAFTVSINARMARGPTVASVDGEVVSPAATAVATAEGPDSDTLSATFTGGWMKRAPPSAAKSSRAAMSMPLPRLGTQTTVGGTGDKDDDLDQLLRVRGSDIKSARGVESDPEPDLQLDTQGDPEPVLDLDLAVAQRNRGAKFCRFRASHGTGLAPVGLISEQVNLPQGVKEGLNTRACSGSLPGAGATSIAGTNIASGSGALTVSLGLMPYRRTSMPNNLAALMRSGTGGLTLGLDGVLHGLSGHGAQSGPGSPCSLGAGFIRSLPLAAYYQHLFRSCQPGAPGAVLVAGGLSVRMGVHSGVAITQVSYNRVAGRTQYSGAALETAKAVCDAAQGGMVLLSHSAFDQLALGRGGGASSGREPPHVVLYMGSHIIKPDPQPQDLYMAQSRELLARVAFLGPVRSSEQVEPGVLERPLGEVSMGVVRVVGLDTLMAWHKETTEEALALFQQVVVSIAMRRLGGHLVEAEPGKVVAVFGHPFDAVRWAAACEALLKEAEWSEALLSHELAEEVQGPVLLDSHMDAPVLMGDGELSRMLFRGLRIKGAVDCAKVKAELLPTTGQLNYRGNLIQNLQRIAAYVSTGQVVCTRRAWRGYERGLELASDVAGAADVVGACLGSHFVRGVGDKVELWSIRLEDFTYAASYYEDMNSAGTGGNSGAEDKYVDTVMAGRLKMLLPSPRHRSVVQNLAAHGVAAAALDGDAVGNRLGASLADTMAAQSRAWEMTGEELQNMLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHI6</accession>
    <name>A0ABQ5SHI6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF1">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="320" mass="35034" checksum="0EE2F5391CC00DE2" modified="2025-10-08" version="1">MSGLEGKVQRLVADSLAEGCKDDRDLMSTLAAYLSKHGLKAVVMAPPKPNAASPLSTRSINAPFLVVTQPRADDHRDEVSGDQDQFTNLEAVAPQMVIVDAALREHLALGPATPVYQRALEGIVPEVFIGTHTRLARLVSSLGPAIAANFSTQGMEQPPWRRKPALLHRWLKAEEEERLMQSEWEERRRVGVERALDEQQQQQQQLLRRPSLSENDLHKYEVAWPDGMPALDAAVCLSSVEVVPIVGFAVGSVAAGREPWFGTQPGAFSESFLRRTSLAESDTSSANSEDVMTGVSPISIFRDAPQPIAFHAHIKIHNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHK8</accession>
    <name>A0ABQ5SHK8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013479</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="315"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="610" mass="66349" checksum="400261C356AFFD3B" modified="2025-10-08" version="1">MMKSALKRLFVKEQVEVVEVQKPSSELSRPPASSSSPECLSNSLSTQLYIHPQGAEDELSDVIVKKEDINVAPSKRSAFSYLSEKLARSSWAGMVHKSPARATLEYQAQPSVAPLSAERTNAWKSPTKRAYSRSMDITVLNHHPVAPIGPQEKLPCVDHLGDSEWTRALTGNIKSVAQVHGAMGYPGVSMPNSPCASQIGRGSTGHFVRHRQSSPMWYVGGAGSGVDVALLLQANQSMPIPVPNQPGAHTPLMDDVDDALESEMRYLDGDGDNYGDDGVHCFTSTLPSVVPPALLEAAAEAGVSLEVDLDTEVELSNDPPLGHGSAGTVFKAVYNGKSCAVKMLSQDVLFGAGCAELHSFVQEAVVMAPLRHPNIVTFLGGSLQPPHVFLLAELCDTSLDAWIHRGVEPRAFAPEGPRRTGPWSESGRCGGQLNTRQMLKVALDVAMGLQYLHGRTPAIVHRDLKPANILIDSNGMAKISDFGLARLKTSNAIHTRAPEVGSVGYMAPECFTSEHGLLTDKCDTWSLGTILWEMVSRKRPFSGLTLTEYYREVVLRKSRLQIPQDDGVCPMALRRLISSCWYDNPEERPSCAHIVAELTRMLKYCPRDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHM5</accession>
    <name>A0ABQ5SHM5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TATA-binding protein interacting (TIP20) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013686</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CAND family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039852">
        <property type="entry name" value="CAND1/CAND2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013932">
        <property type="entry name" value="TATA-bd_TIP120"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12696">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08623">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25782">
        <property type="entry name" value="TPR_CAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="TATA-binding protein interacting (TIP20)" evidence="5">
        <location>
            <begin position="1077"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="332"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="336"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1268" mass="134030" checksum="15E0030B29DED2ED" modified="2025-10-08" version="1">MASTALAIILEKIASKDKDFRYMATSDLLHELQKEFFKADAELEKKLCAVVLNQLEDPSGDISNLAVSCLGCLVRKVADVRAEDMVRQLCEKVAGAGPASGGSSAASKARDAQREISAIGLKALIKELAGGASTGMGSSSQLANSASTIIAGRMLGGLAVHKDDSEVVSQVLDILTELILRFGGLLVAEHSRIRDALLGALGEGRMVMRKKSLQGLAALSVFLNDDTLEGVVSFLLTALQAPNIKPDYARTYIQAVGQISRAVGYRFGRYLSTAVPLVITFCNKAAEGDDELREHCLQALEGFVQRCPHDTRPQMDAVQSTALRYLRYDPNFAADDGGDDEDDMGEDEDADDDGDGDDGEYSDDEDMSWKVRRAATRIISALVSRYPDALPMLYRASMSELVARFDREREEGVKADIFGAVSEMLAQVGATADRYTPGEPNSPTSLLAGDVASIVRASAKQLRDKSPKTRVGVFGVLRQLLAVQAPFVAGQVDALIPGLLAALNDKSSNTGLKIEALSFLRQTIAATPPSIFQLHTKQLSSAVFSCVSERYYKVAAEALRVCEALVGVLRPDPASPVPPAQASFVGPLYSAIMARLSATDQDQEVKECAISAMAAAAARLGDCLQNEMPMVLRVLLERLRNEITRLVAVKALTTLALSPLVSGELLAGAGEGGNGVVGELTTFLRKANRLLRQASLVALEALATRYPAAFEGATLTAAVEEAAALVSDSDVALASLALRFLVSLMSMPGRASAAAAAVVAEKVRAPALALVRSPLLQGTALEVMQSFFAALATVSPAGGDDALLASLLDVGRSAEAASKQAQYSVARCVAALATTTPAKVPVTVELLLKSMGGKDLGSQRLALLCLGEIGGRADLSGLPAVAAAATAVLSAPGSSEEVRAAASHALGGITRGNLGQFMPGLVAQISASAGQPKQQYLLLQALNDVISTLASPAGASKAAELGPEMADQVLSLLFDAAESEEECRNVVAECAGRLALRHPGKVLPALLERTTALSANMRAVAVSAVKHAVVDRPHPVDAELGPVLQRFLLLMGDDDRHVRRAAVVAFSGCAHAKPGLVVGGLDQLLPLLYAQTVVREDMIRTVDLGPFKHKIDDGLELRKAAFECLDILSDCCRDRLEPVPFLAALESGLGDHADVKMPCHTTLSKLVATDPGAVLAAAERLVVPLEKTLTTRLKSDAVKQEIDRHEDMLRSCLRCVDALEHVPGSDNNPAFQNFLRKIVLGTPALKEKYLSIQRERAEAEGGDAMDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHP8</accession>
    <name>A0ABQ5SHP8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_013385</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047011103" evidence="7">
        <location>
            <begin position="19"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="10">
        <location>
            <begin position="119"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="150"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="441"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="616"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="119"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="632"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI68842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="641" mass="69134" checksum="7C46869B301F7F6C" modified="2025-10-08" version="1" fragment="single">VIVVTLLMLRCCCRVISALGRFTGSMHPSLGASSATHRSHTFSVSTVSPQLVTLWRPPAAQGQVMSSCPLAAPTRGLVPTRGFAVAKKRINKLPRLGSVSAAALESIPADCKFYSDTQKSFAGLEISKALEESLAASGFSRPSRVQESAAPHILRGRNVVITAETGSGKTLSYIIPIANLMLKQRAALERYQQEQQQTDPAGVSGRAPTRRYHALVLCPNSALCQQVVATVNGLCGPGGEQLLTAAHINSSNPPPFDAPDVIVATPAGLLTIINDVGGAYGWLWSEEGMQARVRHVVVDEADLLLGNAYIKATEEILTLFKVADRRRVEAKVLEELGLADKSEFDRLPRAIKVAGWRGGVPAMLAEGYKPKRALDPDAKYGPYWRRQYIFSAATMPTITFSDVGSQIQKMYPDATWVSTELLHLSKPQVAHAWHEVSDESFGITLLEAVRSDADYQARRGKTLIFTADGASADSVSELLSGGHVPHVVYHKSRPKPEQTAALEALRAQDGTVMVCTDAAARGLDVQGITHVVQADFAPNTIDFIHRIGRTARAGNSGRVTSLYRERNRPLVEVIRKYISEGVPLEAAFSRARSFSRKLKRMGGVFVPRGMPASAVRGDVQSATIDNTKDGKTATTGRAAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHT1</accession>
    <name>A0ABQ5SHT1_9CHLO</name>
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        <recommendedName>
            <fullName evidence="3">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">VaNZ11_013893</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="3">
        <location>
            <begin position="44"/>
            <end position="243"/>
        </location>
    </feature>
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            <end position="794"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="4594" mass="474447" checksum="9603BC5D2224AE32" modified="2025-10-08" version="1">[encoded data, 4594 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHV5</accession>
    <name>A0ABQ5SHV5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014076</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Sodium/anion cotransporter (TC 2.A.1.14) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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        <location>
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            <end position="481"/>
        </location>
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            <begin position="343"/>
            <end position="353"/>
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    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="481"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="481" mass="50522" checksum="3CAD6E8E4C227125" modified="2025-10-08" version="1" fragment="single">MMLLLTKPRTMTYTGSNPECRVGFPFLDCRFRKDRLCCNSIVLTSSKIAASRGSKLSTGPFSAVPSLPATSPLLSPCLQRDPYMITAYTCCGVSTRRSGAVARASQAMMQYGQHGSPRSRPQPWQVVSAVSAVAMLLCNFHRSVFTALLPLVAGSLALTPAELGLVQSSMLWGYLAGQIPAGRLADERGGDRVLLGGLALWSAATAATAAAAATSPEAALWTLLAARVAMGIFSAVIMPSVAAVTAQWVPPARKAATLAMIYAFFNVGGVLGLIVGPHLAGRSGGWPAAYITAAAGGVMWAVLGSAAVARIGTRPPLPSKRRKGQLPPPPSPLRQPHLPEAPSEQRQEQKLVNEEQVEVPDLPTSTHSLTYARPVVERDEPPSAAPIQAAFTSPSAAAQTAAASGAGRLLPGRAAASETVSISGRRGSLDPPSRMDTPPPAESSQRRSTLIQVAALCWCHGVIGWGFFVLQAWTPMYLQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI73</accession>
    <name>A0ABQ5SI73_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013742</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF35">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="271" mass="29569" checksum="4846A208C71EF4E1" modified="2025-10-08" version="1">MSVRRPSRGSADGPTVLARTNSLTGLTDDDIKSATQRFDLEIVFKFSWINKGLLRISGLDRCINLVELNLTGNQITKIEGLESTVQLQRLILTSNKITAIDGLSHLSRLEGLWLQDNRLASLESLALPQLAQLPALHSLYLQNLDRTAPNALCRTPGYKVAVLKALPNLTNLDGERSPAALNYAELAAEFDAIRANPPAPKKWAVPDVASWLSGVVMEVPMGGGPGEAALQQQHGKLVKVIDECEMLVRVLDDEIAKFDRQTQGKKSETVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIF6</accession>
    <name>A0ABQ5SIF6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA polymerase sigma-70 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013787</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06171">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.601.10">
        <property type="entry name" value="RNA Polymerase Primary Sigma Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050239">
        <property type="entry name" value="Sigma-70_RNA_pol_init_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR RPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603:SF47">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR SIGD, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="Sigma2 domain of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA polymerase sigma-70" evidence="8">
        <location>
            <begin position="673"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="236"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="64"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="736" mass="79962" checksum="573F5F003B632CE0" modified="2025-10-08" version="1">MAFVAPVNHAIKCHGSSLRSIVVTRAVAAPTRPVKSAVPELLDGRLAEQSSVNFQEYTQATQMLNRLKGQASDLELLLDQLNALEASLDDNLLAPPALEELDDRVARQAKRAAKRAERRAKATTAELLMQAAGPSADVTLSQSAILKQPDSRIFPSRPVNDKGRIAGVESETSATGISAVSDVQHLEELFKLTFGEPELPPPEPTAAKMGVVEAMTPTPPAPTSVSVLRPRKVLKKGAPASGRQEAVAAPAAIAPSPRPSTEAASISQTLERLFRHDFTYDDDMFEEGVKGGHHVGPSDEDLHALERQAEDVLQAPDENVHAEASCARELEQAVLAPRAADQLSTLPAGPSLLSLVPASAAPNARIRQARRSGRTQRGRLGRSAGGLSALAAANVRGRIPGNDGTDQFLNSSSHVNILTSEAEMEVTEMCRDFLFLEKVMRQCEKTLHRKPTTEECAAAVGMDVMAFNMRYDAGLKAKELLLKSNYRLVMTVCKRYIGKGMHLQDMVSEGVKGLLRGVEMYDASRGFRFGTYAHWWIRQAVSRSMAETGRAVRLPVHMIEQLTKLKNVAGALAMELNREPSMQEMAEAVGLPVSRVELLLDAARAASSMESAVGSDEGMTIKDAVQDERMTADEAFGSDSLRTDVESMLEGLSEREASVLRLRFGLEDGVEWTLEDIGSRLGVTRERIRQLEAKALRKLQVKTIDVTGKLKDYSENLGLLENRDLASRTSSGTRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SII9</accession>
    <name>A0ABQ5SII9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052671">
        <property type="entry name" value="Acrosomal_SP-10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17571:SF34">
        <property type="entry name" value="ACROSOMAL PROTEIN SP-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17571">
        <property type="entry name" value="URINARY PROTEIN RUP /ACROSOMAL PROTEIN SP-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="324" mass="37008" checksum="616F2F2C5573E8BB" modified="2025-10-08" version="1">MGPASLGEATGISSAYHSTKIVEYIPNYMENVRNLLGFHKAKYFDMQEWETHDVLLTDDGGYDLYGIYPALRRQVPNVMVFNCNGKSLGTMDGFTTDKDLPARPGTSPYKQLFGRCADKENKQRQVFDSAGFDTLINAIQNKLKAGDAPIHVERYQVLTNDHLGIKGGWEVRIMWIMNERMKKWETQLPEATRSRIPPMTEQQMKEQLMRGQLTTEQLTTEQLTTEQLTTEQLTTEQLTTEQLTTEQLTTEQLTTEQSAPKPCAFLQDMFPPAELINFPHVDTMKLDYTSDLVSLMANHAAWMLINNENLVRDMFELAPKPQLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIN0</accession>
    <name>A0ABQ5SIN0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucose-1-phosphate adenylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_013872</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate + ATP + H(+) = ADP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12120"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.27" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02508">
        <property type="entry name" value="ADP_Glucose_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04651">
        <property type="entry name" value="LbH_G1P_AT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011831">
        <property type="entry name" value="ADP-Glc_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005836">
        <property type="entry name" value="ADP_Glu_pyroP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002772">
        <property type="entry name" value="PRK02862.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523:SF12">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE ADENYLYLTRANSFERASE LARGE SUBUNIT 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE ADENYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25247">
        <property type="entry name" value="LbH_GLGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00809">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00810">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="9">
        <location>
            <begin position="76"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI69289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="492" mass="53323" checksum="4BF8F11DCAE62C6F" modified="2025-10-08" version="1">MNVHQTQTRTLGSRTFVCPHGSAVRRPLNAKARAVSRKVQVVKAQAVSTSLETKVANGVAAAASPAPPNFDYAGDISKKRAKPAVPLGANYRLIDIPVSNCLNSNVSKIYCLTQFNSASLNRHLSQAYNSSVGGYNTRGFVEVLAASQSNANKSWFQGTADAVRQYMWLFEEAVREGVEDFLILSGDHLYRMDYRDFVRKHRESGAAITIAALPCAEKEASAFGLMKIDDAGRVVEFAEKPKGDTLQRMKVDTSILGVDPATAQSKPFIASMGIYVMSAKALRELLLNRMPGANDFGNEVIPGAKDAGYKVQAYAFKGYWEDIGTVEAFYNANLALADPSKAQFSFYDKDAPIYTMSRFLPPSKVLDCDVSMSIIGDGCVIKAGSKIHNSIIGIRSLVGTDCIIDSAMMMGADYYETLEECEYVPGCLPMGVGDGSIVRKAIVDKNARIGPKCQIINKDGVKEANREDQGFVIKDGIVVIIKDSCIPAGTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIY0</accession>
    <name>A0ABQ5SIY0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014567</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052981">
        <property type="entry name" value="Ingression_C2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052">
        <property type="entry name" value="CONSERVED SERINE PROLINE-RICH PROTEIN (AFU_ORTHOLOGUE AFUA_2G01790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052:SF3">
        <property type="entry name" value="INGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="264" mass="28175" checksum="DC88AAEA3B8FB466" modified="2025-10-08" version="1">MALEAGEMAVTIEYAKDLKDKDIFGKQDPYCIIRVGSQQFRTRTATDGGKRPVWNETFRFNVINENDAEITIKDEDVGHDDYLGTARISFARAREQGRDTTQAPVYTKHGKQHGFLQVSLSFTRNSSLKAHGAYPGYPPQQYAQYTYPQQIPGYGVPPPAYSAPYGAPPPVYGAPPPTYGAPPPAYGAPPPAYGAPPPAYGAAPPVYGAPPPAYGAPAYPPPGGAGYPTVPQPGYPPQQGYPAPGQYGTPPPAYGAAYPGYPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJY7</accession>
    <name>A0ABQ5SJY7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015259</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050093">
        <property type="entry name" value="ABC_SmlMolc_Importer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781">
        <property type="entry name" value="SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781:SF4">
        <property type="entry name" value="SPERMIDINE_PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="107"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="457" mass="50015" checksum="B30D91A765902075" modified="2025-10-08" version="1">MLSASPTSGRIALRKPLPASGNLQVIPTSVRYQQQGVVGANYGAQRQNSLVACGAASIEREVIGNSPLPPSLSSKATGLATLSIDDTGPAYEGIITDSSTRPTGLYVRVRNMVKHFNTAKGLFRAVDGVDVDIEPSTIVALLGPSGSGKTTLLRLIAGLEQPTGGSILFDDVDATDLSVQDRQIGFVFQSYALFNHKTVAENIKFGLEVRKLNIDHDQRVQDLLALVQLQGLGDRYPRQLSGGQRQRVAMARALASNPRLLLLDEPFGALDAVVRKQLRAGLREIVRSVGVTTIIVTHDQEEAFDLADKVVVFNRGLVEQQGKPTDIIKHPRTPFIMKFVGETNVVPATSLLTKRMRFSTTKSLVMFRPHDIKLFKSPPTEESPVTVAATVADKANLGWTIKYMLRFDDDVECELQLTRDQDENEYNLLLGSRVFVYVNPKSMMGFNKSDIDSTPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK29</accession>
    <name>A0ABQ5SK29_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014522</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000088">
        <property type="protein sequence ID" value="GLI69818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="355"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="272"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="439"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="675"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="850"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="912"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="386"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="401"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="953"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="953" mass="105654" checksum="BCA418A7E3EB0E21" modified="2025-10-08" version="1" fragment="single">MAPVKLPSELFAIATSRSVQGDLALSKALDGIQAAATRLETERTKLVIQSQELQIWDMAREEELLLLRAENEQLEQQLAAVADKVGKGIGRDGVLEGLQYALRKARMERDAEMREADSLFNQLQEAKATAMELQELRTAFGAALNSLKDEWSAIPLLSHETRELRAKADAAVQDPTNLGHKQIGTLLRGLLALASRGSELQHEAQTLRGEKAALQQALDAAHQQLSEQAELLATHPRPAASAPHRVELAGGPGRLSHSTEPAKLSQGDTCKEERLQQQVSELQAKLDEYRSYLQENQEEIEGLQAEVKRLRQASSHASQGAVAQVRIGHAHAAAPRGQSRCGTLGGSSCTAIAQPAGPAWRQQTEQQPTRPPSAPQPVVGTPTRHVPSSASPISTVNYGGSPPLAASPPARASPMSQTVATDFALKVAELQELAIRAQQQSVEREAEILRQQDEALEALRQQLVQQKDAELAELSQQQAKAMAEAQGREVELARQHSRARADLEAQAAKQAAQHAAALAELNSMMAKLVEQHAQALVEREAALAKELQQRLREREMDLQRQHADSLAAQADATEARLAVALAAQAAELAKQHAQDRREAEERQGAQAAARLHELELALERRFSEQLAVRDAEVRRKHAERLAEQAAHLARLHAEQLAQLQTQHAEAIAVREAELLDQYERQLVEREGQLANAHADQLRDLEARFTAQAAEAADAREAQLQKLFADQLRQALRQQQENLEAAHAAVRTRETECVAAAAEQERVAHAQEMARVARQAQEYMRRREEEMVTLLEEQHRRLEFLEQQITIAESESQAAQAAVAARDGAYLGILTRGDDADADRSPTDIEVWQLAAEKSEQVQQLADELEMVRDEAERRREQDVADVERKYVEVVARLGREHEKATEEARTAHELRESNLRRMLERQAEEARDAELRHKEHVERLQAQIVEIRTSLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKD5</accession>
    <name>A0ABQ5SKD5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="605"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="726"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1123"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="605"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1127"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1152"/>
            <end position="1161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1308" mass="138924" checksum="164E9600E210B713" modified="2025-10-08" version="1">MGGDDGVESYIRDAQCRTGQSLGPPANLPAVSNGSSHWWRWSGVPNTMASNLHHRRNALATLLGIPQPITERSMKQQTYSRACQAAWRNCRTRALREGQLQQHMQQFVNWHCFRRGAHSGGPYTRDPSYGQAIGAGPCSLVPVAASDSQEACGSTLHTDSGTLASGSTPLQHQIISSAALSNHAVDFRNYPNRGDLVSCPGVRIQEDKPVQKPKAAAPAQANHIWSCLKSSASRLATCNMEKLKWDEGIAATREGAAASVAASSSGQRRCQPPRFDAMEKEYQDHIMCCLDNINIGAVANTLFMASPSFRANASSSCGPARDPGNTLLESDLQDPGLTGHVNRAMLLLYVFMTAAAYYDGDNESAKDLANSQRHSAILLTNALMSCHGMSPWPYPELSELATLLSNLKALELSGNLPSKPFPESCTSLAMACRLTRAVQQLVETVMAPEVTSSTELALRAEKQYKEAARRGQDEQNRGDSAAATAEDGRSDMGSSAESQALSVQGQNVLAQMEVGGEANAVMEKQRQERQDGEGVGRGNVGQQEAEEAGAKAGTGSIPAPEGLAEELAGIAGRQVTLNTVGTSKRTNYIRAAHANFNAGVSTVPSNCSGGCSSSSGGGSGHGASTGHSAPSTDPTARNATGAADPREPLLNRNHRDLSRLQEELHKMRVLIRHRVYYEALTRHLGLIRSMPCRDWRAAGPAAFVEEYKRLATKEQESLKQLVRPRRVKREVEEKPNMIGNHHQQRRQRSQKQRLWQKDEGRQVTAALFSGAAQAESAGQYAASIAAAPAASGDAADQLPTGPNESRRLDVSLLLDEPLDLLRCEVPSMLCNVVPWNSPAATNEHGAAGGTGVVLGPLESMFLVRPFEDGSLHWRAPADPKAMRCDLARMLLAACLDGHRALFRAVGDAALQLALGATAAANTLLEPGILRIGAGSGELEYLREREIAGGTPVAVTLLGQPPCDPAGSGSGAVVGWCLLLMKPSISTLIQLVPVGDNVILRARQAAGRKISWWPLPAASAATAEHILSSRVKVLRGLLETALAKDSVAVLNCCGGRSTLAEAAQAIAQVHCAALRQRREQVLVFPLLLEEEQKDTALEAAVRMRSAYTYIATANKTVVSTAAATDSAVRLRNEDSESKSRGSTDTAFREPVEASKGAATTSASVKAASSDDWCVTGGGDFSNRSSPPSCSDNSGGAAVDSAAAAVAPNGCPRPSYPSTQTSIWPIVRHFHFNKELCRMLGLLPGQERILAKGILHLGLGLRNDDGRDGGGAVGADADHRYVLPGGPLLFTTNTRLTLVVLSLGVSGSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKM6</accession>
    <name>A0ABQ5SKM6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Minichromosome loss protein Mcl1 middle region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219:SF8">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="787"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="584"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="787"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="818"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="841"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="853" mass="89175" checksum="2EC3511BF3AC6EB4" modified="2025-10-08" version="1" fragment="single">MPSLPTLWDPAGAVDKLPQPFRMIDKILADIVERVVDLIGKREQQRKLEEAAKVNLTFAPHAVMEVPPEASIFKPLGIAGIIAVALPDGELRVFSARDATTTFSDRTHTSTITAIEAGLVVSTSGRLLAAASRTTLSLHEVDIKTCELTLLASIALPTDPDDPSAAVRLYWSDTLGHLAVCYGSGAVALYGVSLPEPNVSRDGLEVLMAMHLHAFGTTRLQELMRVPLGAVKALCLGSASVGCSQLVWQQQHRPDKGAHADRYHKSARGAYVWWEGASRLLLIDIAAATSTATASTIRPSPVNATNGTSDAEAASGASAVKSGTGKAVNTRGGGGISTATPSQTDLLVAAASPVTASAPPPGEPAGPPRTPQVAPLMRDWLLPYNITAAAATADRKTLAWGLADGSVVLWDDRACCATKVLPRLRGAITALAWVNGVTHKLMCASAEGDIFIADIIKPEDSSQKPHEFPQAIHAIHFLANEPFALCICRGHTSSEGDLAAGMTGSGEGSGGVEGLSSSGGCGGVDVLGTKALRPRIIWFNVLEEKPVAELMGPQAGQSFGLACCVPPPPPPSPPTPPPEAAIVPTSPAAGSGSASKSGTPEPGAAPALFGSGSSPGARQAVLAAVTAMGANVQLGPRLVPVPLPIPAGHVVSYPALAVTDTYLVVGGDLVDKVVHSNFADADAVPQRVLLLYVYKVDALLRHLLPEDETHSRLGRVVLDRLLADLEAPKANKRKGKRVTLDVEDPDTPKLPSSMRKPDPFESGASRPAGRSANRHITFGSAQEELFDDARKVPKEAKVFPKETKKGLKTGPEDPVLANKDRRGPDGKPRLAPLGLEKAREAPPPFAERSQSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKR5</accession>
    <name>A0ABQ5SKR5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014743</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF11">
        <property type="entry name" value="METHYLTRANSFERASE TARBP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="4">
        <location>
            <begin position="1799"/>
            <end position="1941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1093"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1307"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1387"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1104"/>
            <end position="1118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1958" mass="206229" checksum="BFEFE3CEAD02C5C0" modified="2025-10-08" version="1">MQSSPIYAMNSVLALRGRLTALSACAEKLDVRGRAAMVSVAFDIASPSQQFLSDLYDVCTLSVASSECRAANPPEDSPSPRGSVSEECVSGCPATQLRATCTAAALHYLSQQDGRVVQQFLNSFFLPLLSHSTALPDKTCRLQTCTGLVALLVSNRLWPQLEQLVRQCCHALEAAANVDPSGGCKLPLPVACTLLTVTVQQLCPLGSAAEELTAGATPTSSGAGGNTVTSSAPAIKTLRWLLSQLRSALRSLLPSPSASARQSALRVLVPITLTVAQACGGSDLASTVQDAWHLALDLLGGDIGGSSRSEAIVITGRNGGGQDLAATCSAVSVCVMAQRIGLALLVSVSGVLLDPAAGVDVRGCERFWDLLRTCLGFPADVDALERKRAMLLLHRLAPPGQMDSPGWAIWAALYDILDEFPIHLVAPVWEKIDQLHTPAPEFRTETVVPKSYIPRKKELKQRKAASASAAHDSTAGAVDTDARPVPQAWQCVLWSRALQHGNVQVQRLGMRTFLQRDWRSADVLRSVPLGFVKGVLLPAIARDIHYRGGAAEAEVLDVQALAAAWLRGWFAAASIEQRIEGLLAIRGMVSGTELSRLGIQQLMRCVTEAARCAAADGQLLRAVAAAATASPPPDASSATASFPAAMLGTLRAVLVYLTTFTGIPYKHSVRQSVISTAAAVVPVEIAGVGSCMRLLLELPPPTLEPGGELRNQVRSWLLGRSNVISADGDAVAAATAALSRSEVATVSAWFQEVLDDYWSQTLHGGAGAGGSAAAPIVQMLLLLDDVEAVCAVVVGRLRTLLYGMYGRPYLQASETHHALVLLRELLAALSRPTVPSALQSAVLGLLVTCCDDICSAASHAADAFLEADPPTAAGSLDRADAAWACVSLALVLTCRSSDPSAGPVTRRLATTLLALLARLAKTPLPAARYSAVVTGLRVATAAAEALRQAAAVSAHIGAIVAERLDPSALAIAALRDSTAAATAAVAASIAASGSGRPDTQADKSQLLSEVEAAKWPLLDAATALAGAVEGTANMAVGGLQPVVVLDVLSQAVAALGEYPEGQVLPLLRCLRRCWVAVARGGPELQAAAVEALGTDMPSPSPPQDATAANGSSSGSSTDPASTALEALCWPVVRALWNALCATPRRTSPLCAAFVNTALLPCLYVQQSDSQLRALHGPQGPCRWLLSRLLEWGGTSSRFLLLLACQLGSFLPLQPVLVECYGREIQLLLLWGSRKGSTYDPDGTMPDGPEVEELSAVAGPPLPGLVTNAAIGTEVAPRVALLCCIHQIAEKAGCIWPPTGTANNVAAAAGSGTGAPQMESPDVAVTETRDESPKRRPVIIFGDSSEWEEQASGLRAALQDPSNMVRGSKPRTVVMGAMATAIATAATTGTDVGDSDGDLADYGAPPPSDPKDPSLLAGQLLWRQLLSVGLDDPLLSTDKVRNGSELHRQKVRLWQSLVVLSSFVPREECGAAVTRMLGQVNGNNPPTVKQYIEAVVAALVLREPSVLFHQILPFLTNYSRHMTGLGSYILIAAQVLLHSPPPVQRHHLRPLLLAICPWLMCHGHNVRTYAQLVMWALLQRFPPESALWAHSIGDLGYLHHMLAFIQSNADLVRLRRNIGDPILHWSPAAMSHPARVFASAPDGVCLAGSPIEQATFEGAPSTLVDRIQTFLVSERHRLRDELADRTAEREFAPQDLSKTIPAAAAADGAAVGTAAGPGGGIGDFQRKITPEAFMATLMLQGPGLAAQGAGGGLGGSSNNLAAVTDQSVLVDLLQQEEAEEAEAAQDWKSSSGGRQLRHDVLLVASLIDKLPNLAGLARTCEVLGAGRLVLADLAATRDPLFTSVSVTAEQWLPMEEVKPAALLAWLERRAAEGYTLVGLEQTAESVRLPDYRWPAKVVLVLGREKEGIPPEVLSLLDATLEIPQRGIIRSLNVHVSGAIALYEYVRQMQFQQLPSAVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLE2</accession>
    <name>A0ABQ5SLE2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Citrate transporter-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_015534</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NhaD Na(+)/H(+) (TC 2.A.62) antiporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045016">
        <property type="entry name" value="NhaD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43269">
        <property type="entry name" value="SODIUM/PROTON ANTIPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43269:SF2">
        <property type="entry name" value="SODIUM_PROTON ANTIPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="321"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="576"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="615"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="659"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="Citrate transporter-like" evidence="13">
        <location>
            <begin position="195"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="43"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="95"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI70604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="680" mass="69854" checksum="D35701B95493906E" modified="2025-10-08" version="1">MLQKTLRLHGINTRMKAALSCQTRPRPFGRAAGALYVAKVHSNANERPEDPATAPAGNLTPSSSPSCSSFTAETSSLTTTATTIITSSTIAEVPPAPAATAAASTSTSTSTSTSTSTSTSKTLISSSHLLPSNDYLQSLEAGPAPSSQAQDRQDASRLVGVCVSVAALALATELNKGWIGSHQELAMTTVFLAGYLGIIVEESLGLSKAAVALILAAALWSIRATAEGLEALDAEMGAALGEVSAVIFFLLGAMTVVEVVDSHQGFKVITQGIRPASKSQLLLAVSLITFMMSSVLDNLTTTIVMVALLNKLCTEPGTRRFLGAAVVVAANAGGAWTPIGDVTTTMLWIHGQISTIATMRDLLLPSAISVALPVAAWALTAPELAEVSTSGGGSSTAPVMVTPAATAAIASRIAAGAAEAVASEARGGWETTAPDHCSVSANVGLSTSTSTSIHNHTPVPSPSGSTDDAVAPPRSNLVLSVGLGSLLMVPVLRAWEGLPPHMGMLACLGFLWLVTDAIHFGEDRRHLTVSQALKRVDSEAVLFFTGVLLAVAALDKAGLLRELAEAMGRVVPQQEMVAAVIGLSSAIVDNVPLVAATMGMYDMQSYPQDSSLWQLIAYCAGTGGSLLVIGSAAGVAFMSIDRSASFGWYARRITPWATAGYFAGLGAYMLQHGVTAAAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM89</accession>
    <name>A0ABQ5SM89_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF1">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="480" mass="50583" checksum="1DCE7D291E733D7D" modified="2025-10-08" version="1">MESAANFLFDLEEANHGSYQCGGDSLSDKAAIEALARRIELTARQPSALGRQLHRDVTALLGRFNSDLHLPEALYTLASALADLGYEVNLRTALGGGNSECFKSLRHMFLVVHGTGEFHGMEFIVEPSLRQHFAIPHPSPDYEYVLSRTPDVFVGGSCRLVPVIQLLCALMADSFQREGLPLPPWRREAAMLSKWLPHPARIHDLTPRPSETQTAHSRLPSVPLAAVSMQPLQIGRTGTAAKATNLPFSPSSGVATSSPSLIQALDGGGVRCLSALSWTVTPPGIIADRPCPSLGCCPRASVVVGFDVNNAGSLGVCRRPHAATPEAGDDHGLDHNYGHSLRGSATCSRPETDARSYSSAGEALDECASPCGVPLAHDDTSSFLFASETSDGNSPLHVYDTPSLVGSPASDAAAPTGAFFLRRHRPTCTGMLPCPSLGVGLLSAKLLLGPLAVMAEAMAVPPSPVPSASTVVVHNTTLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMB2</accession>
    <name>A0ABQ5SMB2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000102">
        <property type="protein sequence ID" value="GLI71127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="38363" checksum="488AF18E68138C0F" modified="2025-10-08" version="1">MDGAVDQRGLLSPQIQDHASIPEVMKLTIPVPKLHIAYDEKPRTPGRSVADATRAYVRNTNKYLQAVHVGGNDGIKCLFVGNTLSGAAKDWYDEWTTARSDFTFDELITALLARFATEVHARNVEARKALAQGTYYMHAHENVPAYQSRFEALVTPIANFSEDDHIFWFQRGLTKTLSKWCAADLSGEPFRTYDALVKFARGTEVRFLTGQDDPKPAPRANAVQAQDPTDEAVTNDHDLGAARPTSAATVMRTERRRGARGGSGTGARGSAGAGPSSGPDKSKKRGGAPSAAAVQNSHPRAKRMRTEDPFLPKWDCKCSELERQKTANVCWRCTADHLTKDCPLLPPLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMD8</accession>
    <name>A0ABQ5SMD8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015625</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1000.10">
        <property type="entry name" value="Guanylate-binding protein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003191">
        <property type="entry name" value="Guanylate-bd/ATL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036543">
        <property type="entry name" value="Guanylate-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02841">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48340">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1 (GBP1), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GB1/RHD3-type G" evidence="7">
        <location>
            <begin position="31"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="492"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="773"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="902" mass="97811" checksum="0C4850086736C31F" modified="2025-10-08" version="1">MGSPLELIKFNVQTGKFEVGTTALATLRKVKGPIAVVAVCGRARQGKSFILNQLLQSSSGFQVGATHRPCTKGLWMWSTPQRINAPDGSSHHLVLLDTEGVDAYDQTAQYSTQIFSLAVLLSSLFVYNQMGGIDESALDRLSMVTEMTKHVRVRAAAANGSGGGEEGLGEHSPAFLWLLRDFYLKLEEEGGRKITPKEYLETALRLVPGTSAAVQSKNAIRTSISQLFPVRDCFTLVRPMVDEAALSQMDTLPRENLRPEFRQGVKDLMALIFQRTVPKRFGLQLMTGPVLAGLVEAYVAAINHGAVPTIATAWQGVAESECRRACDAAEAAYVAAWKGGGDVAAEESELEARHQQSLAAARAAFGEIAVGDESIRQAHEARWMTAVASRYKDFRGCRLAEAAAAVNELLYKGANEVAKAVRGGADLMALQQVITSFVTSYEKASAGPTKFQKLAAFLMETWPAAASQVLDRQSAAAEAAQKALQGEMAELRGKLSAESQRAEQAERHRRDMTNTAKSLHEQLGAAQQEAMRMRAALEQKSGTAAALQQELSGSQREITQMQAELKQKSDVAATLQQQLADSRGEVAQMRELLEQKSGVAVTLQQQLAGTQDELARLRTVLKQESDASVSFRQQLAEAQQDAARLRATLDQKTAKVAALEAQMSEVLVVSERKQASAVELADARVRAAQSEFQTKLSEMTVVLTAERARAEGLAADLQGLAARCASAEGRLAAQEADASEWRSKYMREASLKLELQGQRDEARDSLQLVTVERDSVARERDAARGEAEQLRADLLAETRAKEALQSQMDGYMAEAQAARTQPVAAASYGVDNDENSIPSGEANRGRVLVDASNVVKMSIQEIKDALTQAGYQDEVWALANRRPAAKKADWIAMLQSRSQSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMV8</accession>
    <name>A0ABQ5SMV8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016216</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="3">
        <location>
            <begin position="157"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="517"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1142" mass="121615" checksum="AB153A82DDDB4467" modified="2025-10-08" version="1">MTLAHCDPRWTFGLHPNLWQRRTFRASGHSIALLLIPLVATSLMALDAALAATPSPLPWPSTVTTDISINIKGMLQYRPTKPSGMWMLTSINGSSTSYTLPNQPVDALSGQEIAPGRSVSLTCFLFNVLTTKCSNITDARIYLAASAKPPANVTLRMLVMVVSLSNSSECESQGGAKVTQVKDAFLGPNGYADFFESCSYKKMVFDRQTFTVVSTVIPCSSEIVKCNVDAIATAAKLQLLADIQVSSYSHFAYVLPDNLAPTCNWGGLGELPGAQTWFSPDTSGIFSKGTVMQEILHNFLLYHGWKDGVEYDDSSTAMGYGDSCPSAPELWRLGWANPLVQLNSSSFPLATYTEFILPATYLGPTGVMIKIQPDWLGILYTKNMYLALRVKAAGDRDLLEEFNGKLNIHEVNRSIDNSFLAEGDPKSSFIGAVSPRSSITRFDYKLHLLVGVFDSMNSTIILTICRFVTGPNECNFASPPQQARTTPMQPPPSPFAPSPPPSELLPPPSFPRPPSLSPQQIPASSPSPLNLPSLIPPSSQSPPPSPLNPSRPRPPSPRSPPPSPLNPSRPRPPSPISPSPSPPNPASPRPLSPRSPQPSPLSPPRPTPPSPRSPPPSPLNPSRPRPPSPISPSPSPPNPASPRPLSPRSPQPSPLSPPRPTPPSPRSPPSFPPYPPRPRPSSPLSPPTPRAIELNSSFSQFAGPNVGVNKSFSDKQAMAIWVNAGASSSASTLIAATFTTTITITGQATQALLDIIVDDIADVFINGAYITTAKWGWRWLGDYTDRPIKISLPLGTSTLSLRVQNTGGAAGVAAYLSSSDGKTVLARTNSAWTYTIDAQNAPCAIELNSSFSQFAGPNVGVNKSFSDKQAMAIWVNAGASSSASTLIAATFTTTITITGQATQALLDLIVDDIADVFINGAYITTAKWGWRWLGDYTDRPIKISLPLGTSTLSLRVQNTGGAAGVAAYLSSSDGKTVLARTNSAWTYTIDAQNAPCAIELGSSFSQFAGPNVGVNKSFSDKQAMAIWVNAGASSSASTLIAATFTTTITITGQATQALLDLIVDDIADVFINGAYITTVKWGWRWLGDYTDRPIKISLPLGTSTLSLRVQNTGGAAGVAAYLSSSDGKTVLARTNSAWTYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN51</accession>
    <name>A0ABQ5SN51_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016646</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046300003" evidence="3">
        <location>
            <begin position="26"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="671" mass="71735" checksum="8653B16F646BEBF5" modified="2025-10-08" version="1">MPRMAHSVRSISLVVCVLAAQHVASQDILSKLIPADTFFSGMKKDVVHGHLQRMLQMDIQREEPETVEDCSPAALFEDKKCVAKSGADSKLSPSPTMLSVEAARGAPNCKGPDCVIAPMPSSKATIDMKAPGVERQVKNTKVVTYGPMTQDAYERMVQRKRTALREELQKKGALTAELEAFLQTTGAQLKDAFGIPFPAGPSEPRARDDFEMRFQPQAPSPDPAIKRTTTSVSSETFKATATSSSSTRGDFEMRFEPQTPTMDPNALRSELEAFIAAASPAPQHAIHQGTAIPTTTTTGAADTQNRIPDAAPEPATEAIDMTHPTTVTARTTTSELDPGFANQQDHQRLPLKSVLHDAGVPTSGPARPPMTEQEAARLAAQQVANAAVREAALARKRQMVRELLKTGKKASVQNTATVSPPALFSMGHQKPSDSQQRRRTTSGNNLSTATTTGRATAKVMRDGRGPRTIRRVPPSSWNVTSYLSVAFLVAVVALVLIVLTAVMEDALVARPNNVRNGDVTVALPTQWLALTHNLWRRCLRAFAAARKQFTGHGSDLPMSANEGSGSISRAAAAATEFTQVFWSHLRHQHLGGAVSAAFDASCRTSAGGIVPDRLSVGGAAAAYSSPFAAPQFQQSHGAISPIATPPMSYVIEADGSAAATAALELNLRRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNA4</accession>
    <name>A0ABQ5SNA4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015929</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="16262" checksum="BD8F363B3B82EF3E" modified="2025-10-08" version="1">MELREKGVCHHLYGDELMGLSIPVPKLCISHEVKQRNVERSTAYATRAYLRDVRMYLQLVHNGDRDDVRCLFVRNTLEGMAKTWYDQWTIARENFTFDELTVALLARFAPEVQLRIEAYQTLVRGSYRMRSNEMIPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNB3</accession>
    <name>A0ABQ5SNB3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_016147</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF44">
        <property type="entry name" value="PROTEIN ZNTC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="504"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="584"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="118"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="604" mass="62451" checksum="ED999524E18E1E39" modified="2025-10-08" version="1">MQAHTDNTLGLRISAVFIILSAALLGGVPPLFVKALRSQDSLPTFLIRAFSVGIILSLALVHVLPDAFEELADLGGVDYPLGGTSVLVGLLIMIFIEHGAHIMHNMPHAHANAAGVAGVHSHNGQGHSHNQGQSHADDRSHSHCHRAASHGAGTAVSEPFLTIVAEEWGIENASASLAVEQSGGKRVSVTAGCGERSVHACATVLGDQSPSQLKDPQQQQQQQQQQQQQQQQQEQQQQQQHLENRWQDEQPIQQLGNQAPVAAIAATGPFTRRSAGAAAAVAGMTAVRPLAVSSVPVQSNFRRTCCLKGADAIAAPAKDKTCTTASASSDSDQVARAGSAAVVAAMATPDLAVGSAAGDGTLAAALSRALLRGPSDHCHDCVSRASAPNWPSAVELSSEAKSTVRQRIVAYLFELGCIFHSFIIGLSLGANRTSISQVRALLIALSFHQALEALSLASVIKGGGFSIYKAAAMVITYSVTCPLGVAIGIAMMTSYDPDSTRGRAAQGCLNGVSGGMLLYISLVQLASEDMGKYLIGPPPASDANSGIGADADANATASHGSGGRCGHHHGDGGSGRGIGYRIRAPSFLVFSLGAASMCLLAVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNK8</accession>
    <name>A0ABQ5SNK8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="844"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1046"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1169"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1225"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1303"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1706"/>
            <end position="1770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1954"/>
            <end position="1990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2487"/>
            <end position="2585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="851"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1175"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1242"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1319"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1354"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1954"/>
            <end position="1966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2520"/>
            <end position="2531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2535"/>
            <end position="2560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2618" mass="260636" checksum="563F02A97BC86084" modified="2025-10-08" version="1">MGCFNSKLRPVGHDNNPLNTQPEAQLPSPVPTIALSPELFAEACLQGAKLYGSATSGDATLLFSRLVIARGHILAAVFEGHIAPSAADYCRSHCWDAFVQASASRQQTPVAAALRALDRGFLTSSKHSLESRLASGCSATLLHIDLTARTVTVATVGAHGSVALGSTARDGSGPSGDCTAAGASAATVVTMDASRRGQSRASGTYGGGQEARAVLRAEGSQSEGASGRGPRMSVRGHRPLSCFVTLEAPSAECRRGLSGLVDLVQSALSPAGAELAVVGSSVAGQLSAVSPLLAPEPSTTSIASGGSGHGGTVQFMRRIAGAPAAALPPQALGFGCAKDAAAFENAAAAAASAAVPHAHPPHLQPPRVLATGRFAALGRLHCGCDVRVVSRPLVASDDCLVLLAGCGGTGGQTGAGLRAGTRCSSGAGGVPAEATAKINSSGGGSGGVDGGSGGIGLGLARNGLGASASSGQVGPLSLSPLPNVCSPAGALSATDAVMLAHELVTVRQGGQDGAATERVTEAGGFRGPVGDNRFDVLYDSYDSEAAAIATAAAAAVGDARAAVSTAAFTPRICPAGDRSAMAVASYATAAALRNRAMESLARTSVPVHHASALSAACITIWLDWGTGSLSGAATTATGGGGRLSHAVRLQPGPISLSPPSQPLLSRMAVRWDMLRAYCRFLIARRLRIIASWHALVASASATPHPQPPPQPGTPVASVLSLGSLLGLTGGGGGQGSIESCGGGGGSAPAVNCSASFTSRPPVMMEFVEELPTPAPSVRSRDLRTVGNAAIEATGDGFTATASAAATSAAAAVTTCGNNGAVTLDESSIHSASTAAPYGIAGLVVEPMGSSEDPDEAEDSGDEADEGGRAMAEPPQRLQRTQRSFVMPSGSLFSHHVEAFLGLRTSHSSPDLKNLMAAGRSQGGSRRWDGAGIVGSSAAVAAANLPAGCAAAQVAAREAEAVAYAHGSVYYSSQASTSSAAAIGTSAITGGGGGGSGSHFDWSLVMASVNHTSGHRHGNSAGHTPIPSAAVNRLSSLSYSADATGSNTLGSSGGSAVPAAAGTGTGGDPHTTVTGGYLAIRGSGGGGGGIRLRHISPLSHPALATSPVQAGANAATASWGSPRGTWTAPSVGVNHSGEVGGDNVGAEISRGSSPFSMDMQLPPGVLVGRRSAGDSADGDAEAAAEGEIEPELISEDLPQLRIESTAELAALMAEIIITDDGEVLRTGSGSGGPMPPPYSSRSHGLHHGLHGHHKGSRALSRQGSRGWQPRLAIVREDAREANTSTSCSQQLLAEGLRFTAISEERGSQGSSGAGEHIQGSDRSTSSNSQRSKLANVSNGSASNGSVSNGGSSNGTQPSNGSPTGSVEEQLAALGPGGAAATVLVPPSLPPPAVQVSRWRPADGSSIATRVVGDQAPWIQGGALSTAGHGGLAGVSSIGGGGCSDGGGTGSPISPMLSPFISAPHDQMVLLPSSQLSQPSRNVLGADLQGGTTAGVAAHTASAAVAKDATANTTAKASGAGDSGVWDAFSSGRSFTWRSGAVAEASSFAMGNIDVVPATAAVAPAQLMPLSTASPLPPLQLPLRLRIPAGGADFEEEVLPDPRASLIKHHLIEKDDNDIANVDAAVPAASPTSAIASRHLAGSQFLPSPTHLASYRAAAAAAVAAVKKGTGPDFVLPLAATAAGGVDSPSATAAAVVAMEAFNACYSNSEPSQNRPPLAVTVRPRSESISYHPHQPRDLHQQVSPPAHFLHRPSGSTRSAGGVNAGDGGGEFFLSTQTPHHQLPAASGGRMQANPIYAPKTHTRHLYGLSDVAPPPLAVAGAATRSCCSATAAATSPSAAATTAASFDVGGGTAANTATTIDRSWSVGSASGPLYSLLHSTLSKGSMTAATSTAVATPGCGSTQCSGGRLPTINVFGGNHPPQSQAYSPAVATPSPPPLPAAVAVAVTATATAPPLRSMSRSLSASRMEQSGPGSSGHRPSNNGAAVAEPMSEAAATVLPSLPPECASNSASFSVTLQASRFSDWEPHTAGSAAIASSVGSAGISSGGGAAAAVPAVLSDTAGDGDGVGLNVYQPNDDESPSLPGIDLMGRTFPYMSPYNIQLTPYHQQRPQALPPTRFSRSGFSAAEPAFGLFNAAGTENQVHSGSAAAAVVGVATSGVGSGGGVTPTRDLATAAAMATIAVATSPASMASGVRSDLSPSAAMPGVSGSSQLPRTGFIAGMYGTYGPVAGPSNAFVEVANIYDDGTGTNRSSNISRSDASVWNSTSAWTASTGRTTDMMLLGGSNRRVLASMGGGTGGGSSCMMQLPPGVALGGGGGGGCDTSSLLPYYAAYSSGLLMTADAGAPGSNTQLLMQMGAGTLGGGPAGGGLGGGGVGGGGGGGNAQNSSQLSCSPRQTLVFPGGVMLGGPAGGGGGGGGGGGGEGFIWPAQMMAAALAVQVRAQQQAGMYGAFLTDTQQGHLQQHQQFLQLPQRQLAPVCEDERIAAERPMGHTMLPQTPRGLTAPPPVTSHPHIGISPLQPLRHHHQHHHQQHHQQQENQQQNQQSQLHQQQHPQNQHRQQSTNTVEPGKPQRDVYDRPTAGLYDSGGQAQMIHDTVLDSVSGCFLLGGGSTSNLHTSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP68</accession>
    <name>A0ABQ5SP68_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_017000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000117">
        <property type="protein sequence ID" value="GLI71695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="6">
        <location>
            <begin position="869"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="535"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1087" mass="113451" checksum="F472EE3F7CBA1E03" modified="2025-10-08" version="1">MFQGSSLCTTAPSTICSTWTRTWGCEWASTCRAVERRTCGRSGAPGMPFPPGPPRYWRWATPSAAWRRCMTALGRRADNSSLDSRAILSLVEAPGGEMGACVVNCSLGSWAVGHLLDTPSRTELATLLLRYKPNEVVTAKGSLSPITIAAITRFAGFLGGEGAAAASAAAAPIKYVPLTGNSRDGAAGQRLPAFNVFSHAAVPQGLPLEHNEYGAAHDVAAFIMQVLAAVKEGPSGRSGAGRSSGGNDADNRGGPLTHAGFGRSDLGPALLGTAGRQAVVLQAVAVALAHLRRCFVLREVASAGGARVESLQLGVGLGLNGAAGAADNRYLLLDDRALLTLEVVEGSLGGVEGSLLQLLDRTASLSGRRRVRTWMCRPLCLTADIEERLAVVEAFMHCPNAREAFQMALADLPDCDKLLPRTAQFFASVGFTSAAKRAEPGVGGCSAMGGDDGDVDDPCDARWAISTTRSRCLAAQQLVVGLIGACRAVGDLRKHLQAEWEAPTASLPAIRRAVVAAAKSIPALRLLDSWFGSPALEDPQGADPDQELRPPPGVHAGYDDAGSELRRLQGKWDKQLREEMERLMAAGAPPQLVASGAVVVDHEGAALTWPQKLVPWLRSLGHVPVSPPPTALPDTVTAVSPSLVPLGHELARARQRLGEQCCAALAEVAYAFTASYGEFVQLVDAVSSLDVLAGFAVATDPSQAPPGCSFCRPTFTSAAPPRDGGAPNPSPGAALPRNLPELTLEGLWHPLLAGPDPLELSTEAAGALEAVTVVTPNDLCLGGPRGLPSSLLLTGANMGGKSTLLRAAGMAVLMAQLGCYVPAAVARLDPVDRIFTRMGAHDRMMSGESTFQVEMGEAASGLSAASPASLVVLDELGRGTATYDGHAVAGAVLEYLSRNLGCRTLFATHYHGLATEAAVPAVAGQYTDLPRAASRQGEPPSTSGLRGLVTVAHMASTVTAEGGFVPLHTLRLGPAPDGSCGLQVAALAGLPRQLLCRAQEVADAFVIRTEGTAAYHHGIGADRNQEGDSVRALAVQRDILLKRVREFRRLNAACKEGGGGSQGWAAPEMDALGHFWWRLRQQSPTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP70</accession>
    <name>A0ABQ5SP70_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_017219</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000125">
        <property type="protein sequence ID" value="GLI71823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="463"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="160"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="281"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="417"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI71823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="766" mass="81732" checksum="C278002F69671003" modified="2025-10-08" version="1">MLNRKGAMVAPPPFGFARATNEGMFIRRVSAFTEDAKRPSADEATSPDWGVLDKNFRGVFWSADNLNNVQQHQHSATHPSSGASSFTHLDSPKSSFIGGPPRCTGGGTGEAGPSSSGGCSRLGFGCDPANGSQSPSPPARKATPTPLARHLAERNLLSRSFRRSIDCPPSQLRTRDVPPLQQNVGAPHGGVGGSGPSHLSSCTTPASSVAATSSTSILTSVTPNVATAITNTASSSTPSSTSTIEKLPSFIRVSSSVHTAAAAAAAAAAAAAAEAAANGSSFSSGSCGSNNSPRLPDPSLPQSAFPNGGSNGTAGVPWATHSKDPSSVSSCLTVTSSSRALTPVTGVNPARPSRHILLGARQQRTANCGPFASASDSYTESQAAPRPRASTGSIPAANKQLLMQQQQQLNQQQYPYPYHPQPPPTPPPPPHFTPRLTPPSGLVHLWTAAPVPMRRGEWTMAQYTISRQLYGGYASSVFKARCMRSGQDVVLKVYTISSLSDFLRNQVLRELDIHSRLDHPNVVQLLAAFREGDKLVMVLEYVRGGSLDRARRKLGGRMNEQQALELVMAPLVRTLHYLHTRAIVHRDIKPENLLFTSDWKLKVCDYGVSICLTEERAVTRTGSRDYMAPEVQVCPLKRTPEDNKTNAQLAYGAAADIWSVGVLTYEILVGFPPFPGGPPPLDDGAPNACRRLPAFPSSISADARAFVAACLMDAPVDRPTVYELMKHRWLQTAMQVCIRLYVCLSVHASGRRRRGMGELKILKICD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP75</accession>
    <name>A0ABQ5SP75_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_016422</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Na(+)(in) + H(+)(out) = Na(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF187">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="354"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="395"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="431"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="30"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="478"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI71288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="510" mass="54396" checksum="B760F2DB24040A3C" modified="2025-10-08" version="1" fragment="single">MANDGLEPQAEAAAPTSVAITIQLLVLAVAFVIGRALEKIRFQWMGEAGAALLLGLVVGLILKAAGVGARLADAVAFKGDIFFFILLPTIMFDAGYSLDTRMFIKNVGSLCLYAFAGTTISCFVVGIMMWLFGLWGWCHSMPLLANLTFGALISATDPVTVLAVFQRLDAQPDLYMNVFGESVLNDAVGMVLYNVIAAFLDGKHVNAGSVFAGIGIFAAIFVGSTAIGIAIGLLAAFIFRSRYFYSGPIAIDGLDPGSTEPTEVTSGSNSTFEVGLAVVFAYSSYLVADAARCSGIVAVVVNGMVMNMYVRPNLSLEAAHKIETLFKTLAGLFELFVFCYIGTTMFLQDEEFKIVGYAALSLVALAVSRAANILPCTAIVNLLRPVERHVPPAQQFMLWWSGLRGAMAFALSVEADERFGEYGKVMKTCTFYLIFITVLFNGGTSAYMLQRLKLRAQDTPALILHLQDNLAHMHGFGSEATAGQSEGGRSKGSARGASARPTADGTGKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ27</accession>
    <name>A0ABQ5SQ27_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sfi1 spindle body domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052270">
        <property type="entry name" value="CACF_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22028:SF9">
        <property type="entry name" value="SFI1 SPINDLE BODY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22028">
        <property type="entry name" value="SFI1 SPINDLE BODY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="862"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="927"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1228"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="927"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1238"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1252" mass="135291" checksum="D3732B1B3D880012" modified="2025-10-08" version="1" fragment="single">MSDMIIYNAGEPEVLRAREEQQASHLLSPEETPSPGDTARLTDSARCGQLSYMEPSLQIDADDPLPQTENTDPIPPGDDIDFPSARDSPEIPGASSVMLHQWDLRDSAPPSPRSEQSSQSSFYPPGSSRTSNTGVAAVPEPRSLVRSRDPKADLSTGDVVMLMHQGRPYIAIQARLPYKENASHTCLEPAEEGAALESEALSYLEVVKQGPFVGFRSGAAGNRFMQPRRKAPHRLVFFNHNCGVWEQWDVIAEGWRTVPWSTLPMLLRNRHLPQVQIAVDVVRVGYFTSPGLAGPRSLLPIIPEDPLLEDRNLRRISNLLITEWFKFVDHEKLLREGLEADLAAMADETRELKIKTIQQVEYLRMQINEEMTSLFNHVTLRDEAIATLRGWLQRAQQLAANRMARQRHRRTLIAWRYVVDDIVFRKAAILKLCRKRENALREVAFRAWSQHTRAVIGAAVKLKFAVQRRSHAIMLRVLQCWKFAVARRSWRERTMLTAVQRRSVRRLSTAFIAWWRLTAQRALGRRALLRALHGVNLHWQAAALTAWRAAAQAVHQKMVQADLLLARRATRMQAMALEAWRDQVLRGRVADRYARAVLARRALVGWRHTTGLARAACAAAEAMAAARPAARRREVLTSWHVTTAAAASLRARLNRHVMSRAAVLQGRALAAWALAARARRRRRVMLQRHFTALRLRTAGAVLGGWRAVTTRKVWRRVRVVAALGHASTRRLTSALLSWHAYAAQRRAIAGRKALAIGRLRRMRLAACVGAWHQLATRLQVARTNLLALCRRLLTYTLSSAFREWRQLTASAGAVRSAVARALPVSTRDLLRDVLATWWVSHCAISPVVIDGLGAALRNVASEGRRPGSARATPPTGGIAAGRPVSTGVTPRQSAGQVYDTAVRMTTSLPTNIGIELSAAVMGDLQQHHQQQLSAQQSGLSPEPAAPSVDPTSVMTNSAVMTANTSMSVHEARLPGNPDTFSSTGTWPAPFDVSPIRASNNGSGAAAAAAAAAAAAALAASAAPTEFGFALSNTAGYTSPSARGRTYREALVAGAAAAAAAVAAAGRSTHSAGGGSGGMAGTPTAQHRSSYINIGGGTAGPFVPANAAVIGQGSSPVLVEMGPGHGGEGPLTPVGLPELLDSAVGLSLEGSTPAGLDLLSPVGNGAVDDEHTEALTAAAARPLSHPLPAETLQEHSVLLQTQTYAEAATPAAARTLGFAEDADLQWQQQQQQARLLSGADAAPATPATPALGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLA1</accession>
    <name>A0ABQ5RLA1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000152</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32955"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the PI(3,5)P2 regulatory complex at least composed of ATG18, SAC/FIG4, FAB1 and VAC14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="7">
        <location>
            <begin position="151"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="831"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1021"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1021"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1065"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1085"/>
            <end position="1095"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI58435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1518" mass="160666" checksum="CC29A89834F84FC6" modified="2025-10-08" version="1">MDSQEIPYQRLSIYQTKARLYIVGYCKLRGTAALLKISKQEPTQLDVAEVVGNYSLDEIRAMLSQIHMANAHLGGLQLVTHCCGIIGCFRFTETYYLLLVTRKAHVGSLCGGHKVYTVAATALVPLHPGYQAAAAAGQPSGAAEKRYVKLLMGMDLTKHFYFSYSYNLTATVQQNCRMAQQRGEQPHLQTQGANPPDGSVISVSPSVYESMFTWNAYLTSALRWALSGNPRWTVPLIHGFWEQRRLSIYGRPLTLTLIARRSRHFAGTRYRKRGINDGGKVANEVETEQVVDVGIDYRTRTPLLSSVVQVRGSVPLFWSQQLTALSPKPEIILQQFDPLYEVTEAHFSDLEARYGSPVVVFNLLKSKERRPREVLLRRELATAIAQLNAQRGRSRPHIVYIPWDFSKHAKQSGANLLSEIAAITRLTLDMTGLFVHNPNAPVSAPVRSSGGASLTVGLGFASPRAGNRGSGGGAPGSSRPGVRMPMGSLRRAAGDGGSSMHLAAAAEAAAAAVRARHATAAMLLSRGSGGGTLSSGISSPPPTARPSLDYHNLRRTSNAHSFGSSGGSGGSLSATQWQSGVLRTNCIDCLDRTNVAQFAFGLVAFGRQLYKLGLHDVRDIDGDSSAAFTLMALYEAMGHVLSLQYGGSEAHTIVFQRKKGEWEAATQSKDFVTSIKRLYSNTYTDAEKQDAINLFLGNFVPMPHQPHLWQLDTDQYLHSETTLRPAPPPSLLRSSRSSTVLAPTTPALPVSWCSSERSFGLMPPFDEPTSPTVMVGPAAPARPPQSRFDFARASSDWTAMATVAAAAAALGASPDNESASVVAAAVSSSVTGGGARFPGLPPESTPLPLELSSDDDDDSRSRSYRDLSGPDVDAAGSLGAGQSSSAAAASGAAAYASAAIPFHRAPSNSNFFTSLLPAMGRGGGGSSSSGPGLFERVVTSLLQPGGAGAAARSAAAGVAGGDVAGGGGRPGRQLVSFDQALARPPNQVWPILLHGEEDKQGRWAAVQSAFSFFWTSNAVGGASDGSTRMRNNYQLDDAPRPQVRHSQTALPARLSTPLSPTPSGTHTPLSPTTSMPLSPDPSRHGGAASASANAAAGGGGAANVVSRALTVGTDGGMSVMSDGSPRSPARGLVTAAVAAAAAEGPPGSRSQSLKQILDAMVDVDHMDQHHGRDTTCRTVAETIPALGGTTSGDAGPAVGGPGSADEDDAAYYYSAGDGEAAAAAASGQLDGAGGARQGVRKMLSGPLLNPTVQPIAFVGGSGKQNTPGSAIPVLNDFIEVIGGPASYITDWRCLGEGHTLDPVVRRRAVAEAAAKAREEERQRKLVTAGLEVNDILALGLEDLGLNWELQPWWALEPRPHYVPRPYEPQPGLLLNVVPAHERELRGGQRGEDTLNGQEEPGTGQEIMRVETAVLDADFTPLESSITAVETATAEAAAPGGYTPTPSYILPYEPPPVHALQRKQQVEHMAFVHFDVAQTHARDKFGNFLGSIAQPYGLYGEERRRDIAMYDALCYIEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLR4</accession>
    <name>A0ABQ5RLR4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">starch synthase</fullName>
            <ecNumber evidence="5">2.4.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_000308</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18189"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.21" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03791">
        <property type="entry name" value="GT5_Glycogen_synthase_DULL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825:SF11">
        <property type="entry name" value="ALPHA AMYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825">
        <property type="entry name" value="GRANULE-BOUND STARCH SYNTHASE 1, CHLOROPLASTIC/AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Starch synthase catalytic" evidence="13">
        <location>
            <begin position="592"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="12">
        <location>
            <begin position="953"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="109"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="172"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="327"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="858"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="77"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="239"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="360"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="417"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="460"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="479"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="871"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI58561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1226" mass="127975" checksum="673B0A801CAD57F3" modified="2025-10-08" version="1" fragment="single">MNSNTSSATSSARESRRTLGPSGCARRAARNASHQITFVNWYQQRQRQRPAGRGDPSPGSLPSSFSTTPDEDFLLRARKMQRRIAKLKQLQERLTQLKEEESFLQVYAEASAADSDSDSDADDGDQSSASTSSASLPGANAALAAPAPRLPPPGTSASADELRQWVAAVVAQSAARAGNPRASVRTSSSGESSQRSSRRMIASNDTDIPGVKDHDGQEVGLSRQGPAAYPPAPSPPQQPLQTALTPTGGRTPATAPPRGAGSSSNINNRGGSSRDAGASVPGFNGAVHQTSGGGTGEAAGKDGGGVRRYTVRYSSAASKPVFLQMSPAGAQAVSGSSSSPAASEPHAGTAAPSGGSWLRAPPVVAPSSAAASAAAADLAPAAAAAAAAPPPPPQKQSISPNKGHVSPQSPPSPSFPPTSASATISAAAGTQLGADRSSPSVSAEWRRASPPAAIVPPSREPQSARPSQTPSPQQQLILPPAAAGTAPSTSATTTDTNDAPPSSPAVPAVPSSPRDPNAELRATIFPESLTSTNPYTLYDMPLPPLPLRESWRTAAERMAAAATAGAAATPAAPPLGGAAAGPSRAERPRDLVFVTAEVAPWSKTGGLGDVMGALPRALAARGHRVMVVSPRYLSPSTADRYKGLTDTGVRASLDLGPSRHGGGGVHQVDYFHAHKDGVDWVFVDHMSYHREGTPYGNAHGTYGDNLFRFSLLCLAACEAPLLLNIGGHHRGTPPGLPYGQDVTFVANDWHAGLVSVYVAAKYRKHGVFTRARCVLALHNLAHQGAHPPHCFGSLGLPREWYGALEWVQTPGAGGAQATGNLDRIPTINILKAALVTSDRLVTVSPAYAWEITAGQGEPGEASKEIQRAGAAGKGAPTGRGGCGNEDHGMGLGSLLQQRAADLSGIVNGIDTSEWDPRTDPYLPRNYHEGSLGGKGLCKAALQGALHLPVDPAAPVVGFIGRLDYQKGPDLVLQVLPKLVAAGCQVVLLGSGAPDYEEAFRAAAVAFPANVRAHVGFSVPLSHRILAAADILLMPSRFEPCGLNQLYAMRYGTIPVAHGTGGLRDTIDDVKPFLGEVEREGPEDLDELFATGLAWGRRTPSRSADDPSTNNADYYQPFQDFFGSSGRYGPVDEEDDMYGVDQRDDSDDDLPAWRRGLQGLHRSHDPGTGWTFAPASCEALLDAVTAALVVYENHPDKWRRIQLAGMRRDFSWNRAARQWEAVMEGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLZ9</accession>
    <name>A0ABQ5RLZ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_000227</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF254">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="4"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI58496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="323" mass="37141" checksum="EE35F020A6F33D75" modified="2025-10-08" version="1">MDAYEKIEKIGEGTYGKVYKARDINTGKLVALKKCRLEMEEEGVPSTTLREISLLQMLSDSNHIVKLLCVEHTEENNKPCLYLVFEYLNTDMKKWMDRNGKGPAHPLPTMHIKSMVYQLIKGVAYCHMHGVLHRDLKPQNLLVDDEKMCLKVADLGLGRHFSVPLKSYTHEIVTLWYRAPEVLLGATHYATPVDMWSVGCIFAELVRKAPLFPGDSEYQQLLHIFKLLGTPNEDTWPGVTKLRDWHEWPQWQPQDLRRIFPTLEESGIDLLKRMFAYDPALRISAKEALQHPYFDDLDKVAVDALESQEVRDRMAEAAAANIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMG8</accession>
    <name>A0ABQ5RMG8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000523</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF3">
        <property type="entry name" value="YTH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="2">
        <location>
            <begin position="97"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="520"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="672" mass="70371" checksum="01A7E0FD785AD4F9" modified="2025-10-08" version="1">MDEETLLYEDDYEPELEQELVDDGVHDATPVGAGTEQAEETGIGEPVGTSSKDEGQNAAARGEDLAGPSGRSGANGPSKSKRGRGPNSEGRSSGQGIRYFIIRSSTMQNIFISVRVGAWATTRQNDDKLDEAFRKSREVRLLYSVNGSNAFQGYAVMRTPIGKFGRPVIWENGKQFGNPFGVEWRVLFELPHSETDHIRNPYNDNKPVHIARDGTELPQEQGDLVVRLIQERAAAAGVKPPRQQLAYIDAGGRAGPGPNMAGRGGGLPGPAPGRLVPGGRGPGMGPGMRPVGGAGLDHSAVMALGGMPQGLLGPMPGMGANLEQIANAMAMNNFSPAVLNNMLQQAGMGIQPGVGPMQQPGLQALAAAMVAQMANLLPEQQAMAMAVITANNPLLAEAMAAVVSGGGPGGVGPSGLGAASGVRPPGMGMGNTMSGLNAPLGGPGSVMHGPGGIGPLGGAHRAGGVLGRGGHEGTGPIGVGVGPVESRMLTSQQQLHQAQQQFMNINRDEVVLEGSDGRLRSARSRSRSRSRSPVHGQRGGGPVDFNTMTYEQYLQQYSRVQEEVQRRVGSSVGHHRGRSTERSPRHAREPTQPSQGAMGYNRVASGGGESAIAGGGGAGSGMAAKPGGQPYTEEEYIRVTQTWYASRGQPPPSESYIRQHYRQMLAQLQQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RML3</accession>
    <name>A0ABQ5RML3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SKP1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000564</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends on the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of a SCF (SKP1-cullin-F-box) protein ligase complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SKP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="5">
        <location>
            <begin position="5"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="4">
        <location>
            <begin position="109"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI58817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="158" mass="17975" checksum="30C44ECA88AB4FC0" modified="2025-10-08" version="1">MSGTKVKLLSSDSQMFEVDEEAAFQSQTVKNLVEDAGTDDAIPLPNVSGRILAKVIEYSKYHVEAEKKGADDKPAKTEDEIKRWDEEFVKVDQATLFDLILAANYLNIKGLLDLTCQTVAQMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN38</accession>
    <name>A0ABQ5RN38_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000797</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17986" checksum="AC935EA4F86F9813" modified="2025-10-08" version="1">MEKHHLLRRDPNWHPNGCNLCGQLGHQAANCPNGTINWRQIYGDEAFIMRQPIFYSDIQERIKFKEDGMMDLEARAQAFARGKAEEVGLNWAEIAAKAEELRNKDPSEVIAKVAPAGDDSLPPGWAVATDTNGRQYYWHKKTQKVQWERPREDTPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNC3</accession>
    <name>A0ABQ5RNC3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000888</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF30">
        <property type="entry name" value="OMEGA-AMIDASE NIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046259881" description="CN hydrolase domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="CN hydrolase" evidence="3">
        <location>
            <begin position="54"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="329" mass="35285" checksum="16543B53CA325EF4" modified="2025-10-08" version="1">MSATSIKVAGASALAAALAAAVSRPRIVGRLVRSLVARAAGASTYAPLPPPQKVKVAVCQLNVTADKTQNLQTAKKAIQDAAAQGAKLVVLPEMWNCPYSNDSFPTYAEDIEGANSLSVDMLSAVAAETGVTLIAGSIPERCGDRLYNTCCVFNSRGELLAKHRKVHLFDIHIPGKITFKESLTLSSGPGPTVVDTEAGRLGIGICYDIRFPELAQLYAARGCQVLIFPGAFNMTTGPVHWELLARARAVDNQIFVITCSPARNPSSSYQAWGHSTVVGPFAEILATTDHGPTTIYADMDYSQLEERRSNMPLVLQKRHDLYALLDKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNN6</accession>
    <name>A0ABQ5RNN6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000862</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF60">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="405"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1024" mass="106440" checksum="732C99D8F2BC003B" modified="2025-10-08" version="1">MDSPSRWQPGVLEQDVLPSTSAFVAPGQEDLFLVHVNCLNGSSPAQGKTGSAPASPLQLHSMQHTNIPQGSYLGMSTYLTSQGQPMQPTLAVARSPGSGAQPVPHLYQGSPMRMFPSPTVLSHGLDPGVLIYDTSQQPYVSYDPANQYGLQHQQCMPALGQPSSVQALSSNFMAAAGQVPVGEAPISFHSYGLTAPGPFPPSYLQQQQQQSGLPASQQQQVLAHRTNTTDQFGVAFASPTRLPAQESPPSSGSCRPGNHQGPAVPCPAENLIGGLVTAAQIVTRPAVNELSRAGVVREGGPGGIWGPDVPTLPPPSGGPAAKVAAGVNLMSSPEPSSPNGFASGRAVVGAGLLHGAVRHVVEELRTSPLVLPSDYGTPAHSSIGVGRTSGGAAHGNGSGEKNTALQRYQAQILSLERQNAELQKRLVDTHAALEAARQARQAVEAKQDAMADQVAQANHSAKSRESALETARRANDALSGELRSCQEELAAVRNALAAAKDTAVQWQDRAIEVGKELEVARKKIETLGEEERRLSSENMALRKEAQQLNDVIIKGRIESAELREKLRSAQQETARAASTAQALASRAESEQEGAATQIALMRNKVVELESRNSQLLYDMAGMREEAARARQAADAAKAALATKPAGITSDAGLGTRQHHSHGLMATGWTAHDAQPTGSGGGPGGGTGYGGLYCGGGVGIGGSYSNTIGNGSGASYRGPDYGAGGNGNGTVFGSGVNASSRPALMPPTTTSAVPSLQPDQDTAAAILRDLPSDVAFSKLDPETFRSMVRAEAEALARGGSTGLYGGTNSPMKPSYGTRYGSTSNGDLAVEPSTGTLITGVQPPPGMAWAEGPSRELMLPPPPLPDARNIWRAAPPLAEERSVGVGGATNSKAVNNSAGNGYTTGGGGGSPPYAVNIVDSSYAYRRPANAVGSGVAPHGGNSGADTPFGTEETVKEMMARSKTLEDQLMALCAEKGGLEAEYARMPLGAGRSLRERNRKQVVEQRLELLNKEISSVRMQLKRLGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNP4</accession>
    <name>A0ABQ5RNP4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="7">3.4.21.92</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_000579</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001368">
        <property type="entry name" value="PRK00277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009205">
        <property type="entry name" value="PRK12553.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381:SF50">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI58813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="347" mass="38955" checksum="18649E31907EA4A6" modified="2025-10-08" version="1">MVATAYLCGIQSTCPLAGGPTRRRAAGLRVRAQLWTPEQPSHRTKGWDRPWIQANSQPIVAPRTAEMQGDPFGLLLRQRIVFLGGEVEDFGADALISQLLLLDSQDPTKDIKMFINSPGGSVTAGMGIYDAMMLCRADVHTYCFGLAASMGAFLLGAGKRGKRHSMPNARIMIHQPLGGASGQAVDIEIQAKEIMYHKANLNRIMADYTAQPLSKIEEDTDRDRYMSPLEAKEYGLIDHIIGGEEAVFNVKGSLKKFPKVKEEFVTDRDDLIQRNIMDGDPFLSETPSWRFRKPETEPYMPSQTPGSPWFRVKKVPKEEYKEMLERREQQALPDGKKSVKERIDEAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPC4</accession>
    <name>A0ABQ5RPC4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_001286</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="180"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="621"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="668"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="803"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1027"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1174"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1272"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1388"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1437"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1520"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1687"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1923"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2013"/>
            <end position="2039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2088"/>
            <end position="2119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2216"/>
            <end position="2235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="621"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="815"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="844"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="880"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="907"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="925"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1030"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1087"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1121"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1174"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1193"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1287"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1437"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1456"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1520"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1530"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1692"/>
            <end position="1706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1707"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1726"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2283" mass="228159" checksum="82C75A9556288474" modified="2025-10-08" version="1">MSHQVDFSLIVPAPIPSVRALQASPEVLTPVRHGFLWKRSRLFKTWISRWFELDTSGFLYAVSPKVIKREGQSVLPSPVGAVRQLPPQRYRNGAMMYGIRVISVGGSCKDLYSDDLAALDAWALDLSRCTRTAREQAAAGKAAAALASQPSLLLLPPPPPSPLAASAGAGELQLSDICEMDWSHMLGSGLFACVLRARMHVTGEAVAVKIVKAESFREYGDIIEREAAVWAAAGQHPHVVRLKQTLRSSERMYFISELCEGGSLVERLATSATYCEREVAWLVRQLLAAVEHLHSSAIVHMDIKPENLVFATRREDSCIKLIDFSLASFFYSPTDPGGTPDFVAPELLNHPEHYSKNGCGPEVDMWAVGVIIFFLLSGQTPFQAPTLEAVLQRVKTGEWAFHGRRWALVSEGARDLVSSLLRIDPSQRLTATEALDHPWLRRPDSLSQALLQDAVAAFRLAAAASMQQQRTSWVKQASGDLMTTNGTNLSPHTSLSRFAVMAGSVGPASFGGGGAAAVATGMGINAMNSAVMGAALTTGGGHCLMGLLSSGAADAVAAMIGPSGAAASSAVMISSSASQHQLNQAAVSVGGGFEFGGGSARGSLGGGQRGSNTSLTVAAGPAAAGVGSGSSPTTGGGNSPSNGHLPQQNSFTAGVGSRYAAAVAMAPHCSGSGSAGSPPRLPASAARISPHNSHHSPGACTQRSPRSPLGMRTRRASDTMAYTLAITERHESIHQMQAQGIAVPPAAGCIGWPGLPSAKARRASELLPVYGTAAAGCMAPSPSDSQAELAAASAAVAAASASVDRGNHSAPGTRYVHHNHHHLHGHHHPHYGHPLPLRGASGLSVMTSATATGVSTPQGDGRPTGGRSNPELFSMLRLQSSSGGRSIVSSSTETTPHTSSRDCTAAGRNSWTNIPSQTSAPGPAQIQGQPHSQQGQLAGASLPPCDQALLAMSKGLRQGVPGKSQSYNAMPSIWVDRPAGRGQSTGQSYNVVSPAGGGAGAMITQRRASMSFTLGASVSGLALMPHGPSPLSNSSGGRNFLTDAQTGGGGAASATAAGASGGSMPALLQLKRPSRRISGGGNLGTRSSSSTPAHASPANSNTSLQAPSHSAAGGTSGGGSTAARSSVSGAPNAGGTGFPSSSSMYAAVLRSNLSGHGLGGGGGGNAASCSGGADGGGGGGSGSGGLSDEGVARQSSLSASGSVTVGNNIRPISTATGSSPPPTRLGSSIGPTSPPSPLSPAAMTNIVFSGVGVGGSGRCSSSNIDPFLLVGGGGSWDNRPPSDRTAPSPPVQPPSPLPTLAVEKQLMHEEFRVMYSSGSNAISGSNSMVSERLATQGSFIEATLMTANFNGSGTGHTSRSGAGGTIEVSSGPHGRTQAATMDVNGNLKGGTTDLRRLQQPPSSTMALSRGVASGTIDSENDVFSIALNIVADGSDGAGSSGNGAGGGGQVQSGVQLRSQPRSGSQHSLVCSGTSQNSEGSPRADCNQVAAGGAIIGNSLRSGAAAGAGAGANALAVSASGGAGGGGPGSGTAPTGATDSGANSIMETLRSAGVAPRASSRLVLETIPSEDPDSLSGLLRSVREERAAAIASAASGAAGGSAAIATSASGSSVGAGGFSSGPGGFGTGTPTVLQAVARPLAGQQQQLFSPAPSPLSRRQVRAFLSPQASSTSLAAVSLPPSCKSVLEPSSCDSGGGLGNGGGGGGGTKQQQQHVRASSGGSSGPALVSHGSGSVRSTGSVRSGSVRRSATANNLRLHATTAAAAQAAAAAAATAGDCITSEPDPFAAWDYPPPSLGHPRQRATSAVGLSGPSLMALLAQAAPLAQAAQAFAAVQIANSQSSSAGSSFTSQRSGIGGGINGGIRAAVAATPAVAPAASTAADGAAALADVARTVGAAMSQADVRRSSTATNLNLNVQATQQLQTQAHQQQQGQFNAAASRGASAGNSSSSNKYLAGIVAQAAPLAQAAQTSAVAQAAASRAASCSSHQSISSSKAAAAVLIGAGGGGGTYGLHGRGTGSGAVTPTSYGSESPAGSPGWARRATTSNDLRDRALSVQLSGNSIVHRLASQQGPSLATAALAGAAAGVGLYSSSPPREPGSPASLSSRGSTFPVVPSRLSNSGNCTPTARCAGGGIPSPSHVLSPQGSLAGGGSGSVGAISQRALMAVLQQQQQPQLSGPSVVYHQQLQQMQQVQQMQQQLQLQQLQHFHNMQQLRHQQQKGVTGAAPGRTSRADSAAAGGLGLRVSTSASVGTIDTATQAAIMQEMSKVTVKRSSCAQMPPPHLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPX0</accession>
    <name>A0ABQ5RPX0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="133" mass="13444" checksum="8CC623A06C72AD98" modified="2025-10-08" version="1">MTHMFKSGSSQCIQLNVVDLIVDLDHPVALLPAQHKVGGEGGPMSPLATTAGAQGSGSTSVNSRQQQQQQQQPAVETRRSTVTGGGGDVVGAHGSSCIGSSSGAAPRASSPTGPLESLIVRPKNFVPGETEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ81</accession>
    <name>A0ABQ5RQ81_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001673</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046220688" evidence="2">
        <location>
            <begin position="25"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="118" mass="12545" checksum="FC790434FD8000C0" modified="2025-10-08" version="1" fragment="single">MLLYCLRTTLLLLLVFTAASLSLALSSVGARDRDNGQVPNQRPTTTSIKGTSLIRRRTATGEPQPTSDLNDGALNTHLAYRRGRMDNSSRHGASGEVDAADAAADKTLAATTQMANET</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQG2</accession>
    <name>A0ABQ5RQG2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042984">
        <property type="entry name" value="BBS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46883">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46883:SF1">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="726"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="969"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1237"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="642"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="783"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="969"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="982"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1244"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1271"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1280" mass="132909" checksum="9CE2174FDAE5C8EE" modified="2025-10-08" version="1" fragment="single">MSRFELARKLQATCGVLRSFLGPHKVLKCVIDSTSDARNCLAGTVQAFLLAADGDPTTTNLLLEALEGQAPSAADSPASGPGPGSGATWLAVFCGQLLGHVLDLVEIQGVPYECVRAGVLQAIDECCEAVRECAIELPMVAERWDEQDLMSYHEAVSLLMVGTEAVSTMESASLPVVSTASATPHPSTSAPDWDLPAFPPVASAVSLSTAGYPIDSAVVDLESGEVKTQADAMKLWTGPAYHPPPTPSMLPQLQLPQQVMMSAATAAAPSDGNGGGIQHSQCPLPQEQQQLSLPREGQGQAHPQLPGQVSQLQVNGSGRTSDGVYNAGRAPAAGRECGKVHSEYVGMPLEAEVELAALEEEASWFFNDGELQAEREARAQKSLLQHLAAGLTAAGVPAPEMRSLKKAPAFLQDGARDTSGAFQTKARDASCIPAAGRSSHSVVKWVAEGRHRSDGGGDSNFDSAGDEGGRDSSKLGTHGERDAFGGLEERTADGDTGRVAAVGCLQIRDLHPKAHARTPPTSCHPSTLKAVLDDDDDEFGWFQSYDVNAHYGGLATGLRPHPTRDSTTVLIALESPSTAEPRSPVPPAAEGDVNRGSEAAGVAGTAGLTDPQAGGLRDPKSWAAGPQDYAEGAPGKGNSNSWRERGAPIREGRGRNVAEGKERGLSKGGMPLDAEVELAALEEASWFFNDGELQAEREARAQKSLLQQLAAGLTAVDSVLLRGDGSNGEVHGQGGRAAEGISTGSTNAVEEELELSGRKSTDLQDTDGNPHHLHCVTAAPGASPSPPTPPLPPLPAPVATPTRYFLDSTSGRDEAPEEWGTASRGGRGSGKCSFEITAARQRLSPLTRLVCAAAHGLSHGRSLEMQLAAAALLLLLEPLVRRQPGDVRPPLAAKTTSAAIAAAKEDLSAMSAQVRQAAAALQFERCVLTVEMMGRSAVAAAAMRGVVVPLATLSTAQAALAAEFFTALPSPPSPPPPPPGNSSPSAAHTATPTAPATLRARKFTTVFFQGALQVDRAVYGPIVITDAAGLVAATSDARVEAFASHLLEALKPHGVQLLLASGHIPDALSAVLQQLSGGAMLVLGGIGLRAIRAAAACCGAMPAASLSSLDTRRNVATHVAVELVQGGLGLGDYRTAAGHRHTEAAVLMRITKAPRTHLGNPSGGDCDASVPQVPVLRPGWVTIVSSHSLASQLEVQGVSFRTCFNRLIAALRKGHVLPGSGAWELAAAEHLTRRAKQLEAHHASPPLLQPLPSGQQSMQPPQSLLAEGRHGNSTIGEEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQQ5</accession>
    <name>A0ABQ5RQQ5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001442</name>
        <name evidence="2" type="ORF">VaNZ11_001446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="228" mass="25217" checksum="70736E4C2B3D456C" modified="2025-10-08" version="1">MSSYRIKLVLSDAVRKYLYEEKLNLVVGKAVGTVERPTYRTAWHVLTPSELAQTVELTWKHNYTGSFTTDKIYDRFSKYTTQGNDIQMAAGHIYDVTAYGTMIENPNKPPRPNSNRFFFCNGMGYGRVFRPVLKTPAPGASASSPGAPFWLGDHVQKNGAVIATPVEKVIVWFGKYNAGAAILEEVMTVAVEFDLTEITSGSAEIADDLSAWKNLSPNAQEVDVVPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRV3</accession>
    <name>A0ABQ5RRV3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Component of cytosolic 80S ribosome and 60S large subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002042</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11421" checksum="C4D374891F2F472A" modified="2025-10-08" version="1">MVNVPKTKRAFCKGCKKHMMMKVTQYKTGKASLYAQGKRRYDRKQSGYGGQTKPVFHKKAKTTKKIVLRMQCQECKQTCMKGLKRCKHFEIGGDKKKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSN8</accession>
    <name>A0ABQ5RSN8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TNase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002622</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF26">
        <property type="entry name" value="BLR1266 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046181504" description="TNase-like domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="3">
        <location>
            <begin position="33"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="378" mass="39943" checksum="B1671C1BD13BB172" modified="2025-10-08" version="1">MTSSFAPVLILLASLLPLGVWAQQNNNITGQARAVDGDTLAFKTDRVRLYGIDAPESAQYCLDALNVSYACGNVSKQALASKIGNSTLTCIVKDIDLYGRSVSICGLTSATGYFEDVNAWLVSNGYAVAYRQFSKKYVPQEDQARAARLGVWQGQFQMPWDWRNSDSPPPPKPSPMPSGVASPPLLKGSPPLSQPPSLGKAPPPQSTRPPASSSPPLAVNPSPPVAKLPPPSLMPPPSVKLPPPVIKSPPLVVKPPPSPFPPPPPSPFPSPPPSPFPPPPPSPFPPPPPSPFPPPPPSPTPIRQPPLPSLPSTCGDPVPMCPTGPAIKGNIASSGEKIYHVPGGKYYDVVCISLKSGERFFCTEAEAQMAGWRPASQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTE3</accession>
    <name>A0ABQ5RTE3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PhoD-like phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002671</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043904">
        <property type="entry name" value="PhoD_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689">
        <property type="entry name" value="MEMBRANE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689:SF1">
        <property type="entry name" value="PHOD-LIKE PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19050">
        <property type="entry name" value="PhoD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoD-like phosphatase" evidence="2">
        <location>
            <begin position="305"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase" evidence="2">
        <location>
            <begin position="578"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="788" mass="84832" checksum="B62B89C7BC955425" modified="2025-10-08" version="1">MGCAASTAAPSSMPPHPTSSRAAGSSKQGPLDQQASSASNRESSSSLSAADPSPFLEYYTLAALCNRELFGPFLKLCNYDPDTGRLQLSLLLITTKAVQQECEEQLRIFWWNVSGTQTAGSAPQPQPQSPCSRGAAECKTEATAVEGIKPVCEACDGIYSLNDGSGAGGGGLTNGFETAGGESMGDGSRATGTDVDGSGAGGGEGTGGCSSWGDVAKGKAANGGGGDGGGGRSSCQDGIGERQRQQEQQQQHVGAAVPLGAETAAGELLYSWRNWHFWRFRFGTVCGETPKRLVYQVSLLPECSYSIAVPALEEPWRMAFYSCNGVDASHILPCEREVAAESALSLWQDLARRHEQQPFHLLLGGGDQLYNDGVFEGPLLKGWDRTQDDRDTAAKAAIPFTDEVAWEVEEFYFSHYAAYWSQTAFKKLFATIPSFNSWDDHDIVDGWGSYPGIINDSATMQGVFRAAQLFYLLFQHHTTLDRLPGDGYWCGPTSCALLHLGSSVALLVPDTRTCRSQDRILPPSFLHQAQARLAALPPTVQHVMVLLGGPVVYPKLPIQDALQKLNDLFTGDTLISVIMQKTGLAAKVTKRFGMVAYLDDIIDQWSAKQHHQERDHLLQTIIDLSAERGFRFTLLSGDVHCAGYGMIHGRGDESQGDVEDMSVPSERQRAADPRFIPQIVSSAIVNVPPPAALLKLLCLTARKPECILKDCVHRMVPLFGPTDDIDSFLCGRRNWCEATLGGPDGNLSFSWRCEVSRGAAEVEVFSVKVPPLSGGSGSEDDCKNSSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTN3</accession>
    <name>A0ABQ5RTN3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Clp R domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_003138</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="entry name" value="Clp, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF155">
        <property type="entry name" value="CHAPERONE PROTEIN CLPC1, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="Double Clp-N motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="11">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="10">
        <location>
            <begin position="379"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI60905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="799" mass="88468" checksum="B03C6EED06E6BBB9" modified="2025-10-08" version="1" fragment="single">GIAAKVLKSMGVNLKDARVEVEKIIGRGSGFVAVEIPFTPRAKRVLEMSLEEARQLGHNYIGTEHILLGLLREGEGVAARVLETLGADPAKIRTQVIRMVGESQEPVGAGVGGSQTQGSNKTPTLQEYGTNLTQQAAEGKLDPVVGRKKQIERVIQILGRRTKNNPCLIGEPGVGKTAVAEGLAQKIATGDVPETIEGKQVITLDMGLLVAGTKYRGEFEERLKKLMDEIKQNDDIILMIDEVHTLIGAGAAEGAIDAANILKPALARGELQCIGATTLDEYRKHIEKDPALERRFQPVTVPEPTIEETFEILQGLRERYETHHKLRYTDESLMAAAKYSSQYISDRFLPDKAIDLIDEAGSRVRLRHIQLPEEARDLDKELRQVTKDKDAAVRGQDFEKAGQLRDREMELKAKIQAIIAGAKEASKAEAESVEGGGPVVTEQDIASIVAQWTGIPIEKVSSDETERLIKMEEVLHGRVIGQEEAVSAISRAIRRARVGLKNPNRPIASFIFAGPTGVGKSELAKTLANYYFGSEEAMVRLDMSEFMERHTVSKLIGSPPGYVGYQEGGQLTEAVRRRPYTVVLFDEIEKAHPDVFNMMLQILEDGRLTDSKGRTVDFKNTLIIMTSNVGASVIEKGGRSMGFFLRPDDEDMEQDMSYSRIKSLVNEELKSYFRPEFLNRLDEIIVFRQLTKKEVKQIADIFLKDVFKRAEEKGIKIEVTEKFKDRLVDEGFNPTYGARPLRRAIMRLIEDCLAERILMGDIKEGDVVIMDVDPDGSIAVLAGDKKMRADIDAVPAGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTS2</accession>
    <name>A0ABQ5RTS2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="196"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="91"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="230"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="263" mass="27769" checksum="5AB09BAB856FFEAF" modified="2025-10-08" version="1" fragment="single">MAEQLYRKRILPATSSNVDDLVSKNVLSQLRRDPPPRPPVHGHADNGKSGTGGSSGSTGVSGIGGVGGGMGQTKGAPVEASDLISTMAKRLGALEREMKERQSLLQQVQADNTMLKNKLKVAEEEIAKRALAAIEPRDDSAAVMHLRAENARLRAQLRYAWKQVEEMKGFLNDYGMVWMGEPEAEAELEAHGFRNADEANSSGAPAVGPGFLQALSPEASDPWGPRRNSHSGAAPGPSSVAAEATVAVIGRPREPSGGSFRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTU7</accession>
    <name>A0ABQ5RTU7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NEDD8-activating enzyme E1 regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002954</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01493">
        <property type="entry name" value="APPBP1_RUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030667">
        <property type="entry name" value="APP-BP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF29">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039099">
        <property type="entry name" value="APP-BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="5">
        <location>
            <begin position="12"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="565" mass="60939" checksum="64B7F2FA19322D41" modified="2025-10-08" version="1">MAPVHDPKAKRYDRQLRIWGTHGQQRLESCSICLLKCGPTGSETLKNLVLGGIASFTIVDGEKVDARDLGNNFLVSASSLGEPRAKVVTELLQELNESVSGSYVEEVPEVLIADNPQFFHSFDLVIATQMREQDMVKLDEICRSTGRAKLLIVRSYGLVGYLRASLPEHHIVESKPDSQLDDLRLHQPWPELVQFATSFDLPSLDDIAHSHMPYVVILLQAAERWREAHDGQLPGTASSDKSAFKALVASMRRTADGIPLQEDNFDEALKAAFHVSTPYRIPSEVRALLDDEAAGPGLRPGSNEFWIMVAALKEFVESEGAGTLPLEGSIPDMHATTEMYLRVQHLYKEKAERDVAAVEAHVRQMLGRLGQPADSISHDTVRLYCRHARHLRCVRYRTLAEETGSETARGNVLAAALSAGGGGGLGFGGDMPPGSSDAALYVLLRAADRFHALTGRYPGSPAVDPQEDLPLMRQAAMQVLSEAGVGGAAKSPDSSSGSGAGSAVVSEDLLAEMCRAGAAELHVVAAFMGGVAAQEAIKLVTRQFVPLAGTLIFNAMACTTTVLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU41</accession>
    <name>A0ABQ5RU41_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="21867" checksum="DA3485392ACC4B32" modified="2025-10-08" version="1">MGDPVYPSTLRYRDRLGVANEDVYKYNRLYATQQGSDVWERLTSDASLRQSSARSRPPFQEGQAQVNNSYKNSGFDSNTCPAQYTHNSFKQGLYQHDTPIGQQHKITAGRFKQAYPLDFMGQIRPETETTNQAMRMLGTYAADQAHADRLGLFIPQGCPGGKPSYHPDVTTGDYGLAPTLPRKGLGQTLTDGRNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUQ8</accession>
    <name>A0ABQ5RUQ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M3A/M3B catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_003723</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF84">
        <property type="entry name" value="SACCHAROLYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="7">
        <location>
            <begin position="251"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="727" mass="79953" checksum="469349D70C335ACB" modified="2025-10-08" version="1">MNLKHHMKPPCPTRRSAGRSIKKIHHSADFQWPHQAFKLSSVGQERCVASDELTRREAVLASIATTIFHRILATQGSRQATLSLAGFTVSAAPNRMAFAVEVGAQELINKLNASYEKAHVQYEENFWATKMGLKGNSSEALASSKTAYEAFLGDPTNLAAVRHALAEGEKTAGSLTPAQERVLKIMERTFKCYITEDPKAAALKEKINNLEAALAQSRNQMLLGYTDPASGEFKKSSSVQLRNLVRVSDAEATRKACFEGLRSIGPFVGAQFVEIVKERNKLARLLGFEDFYDYKVTAAEGFGKSQLFSILDDLEAKTAPIMTAARERLAREKGAAALEPHNMSYALAGDTEKAQDPFFPFEQAVDVWARTFASLGINYMGSTMTLDLCDREGKYSNGFCHWPQVAWRQPDGTFVPAKANFTSLATPSQVGSGRTALATLLHEGGHAAHFANIDQPSPFFSQERAPTSVAYAETQSMFLDSLIHDGAWLGRYARSRSGDVMPWTVIEEGLKATQPYDVFALRGMLVVPYFEKALYELPEDELTVERLLSLADEVENKVQGGLSPRPLLSVPHPLSDESSCYYHGYVLAEMAVRQTRAYFKSKYGKIVDNPAIGKDLVEGYWRPGNGAAFLELVKGLTGKPLAADDWVRDLEKPLDQAIREEEEDYQAALAAGPALKPGDAFDLGMHVRLVNGDELIADSEADGSLAAACDKFKTWVDEKYFPKSTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVZ6</accession>
    <name>A0ABQ5RVZ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein dpy-30 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004019</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22965">
        <property type="entry name" value="DD_DPY30_SDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049629">
        <property type="entry name" value="DPY30_SDC1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037856">
        <property type="entry name" value="Sdc1/DPY30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23356:SF16">
        <property type="entry name" value="DPY30 DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23356">
        <property type="entry name" value="DPY30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="118" mass="12439" checksum="7557ED3AD4F5AAEF" modified="2025-10-08" version="1">MGDDVAMDTGTQPATSLPAVAATTPEAVKAAEKSAVGVHKKINLAAAPIRQYLEATVVPVLMQGMQALCKERPDNPVEFLAYYLLRYNPHPNSKPNPQAGVIEGDGATKPEAQTGAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW57</accession>
    <name>A0ABQ5RW57_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_003650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12232">
        <property type="entry name" value="RRM3_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="117"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="451" mass="47890" checksum="4B46C0CED051EA65" modified="2025-10-08" version="1">MGSRDRSRSRDRRRSRSRERSRRWDRDRRSRSRSRDRRRSRSKSRSRSRDRAKSLAYDASISAVQAMAALQQQQQLQRQLIAQQLLLQQQAAAAAAALAPPVQQQNTSAATADRKAREIYVGNLAIGVVTSDMLKELFNTILANQVPDPANAPPVVNVNLDSAAGRFAFVEFRTRELTDAAIQLDKLELCGRQMNIGRPKGYVAMQMQQQTAVAQAPTQLADAAKLGQAQVLAAALAGAMPAPANTLAAAAATLTVTTPTNVVLLENMVSCGTIRDEIERKEILEDVQQEVVKIGTVIGLAVPMPPAHVTNQEASRVYVKYSTAADAARCRNMMDGRKFDDNAVKAMFVTEGEFLRAQAGEWVAKPGLLMPAGMPALPTVTTATAASPALAAPVPALASLGALGALGGAAAAPATGHVPIQQASGLVLPPGFTLPAGFTLAAGLPGLGLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXR3</accession>
    <name>A0ABQ5RXR3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">monogalactosyldiacylglycerol synthase</fullName>
            <ecNumber evidence="2">2.4.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004344</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Plastid</location>
            <location evidence="5">Chloroplast membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.46" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009695">
        <property type="entry name" value="Diacylglyc_glucosyltr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050519">
        <property type="entry name" value="Glycosyltransf_28_UgtP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43025">
        <property type="entry name" value="MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43025:SF3">
        <property type="entry name" value="MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06925">
        <property type="entry name" value="MGDG_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Diacylglycerol glucosyltransferase N-terminal" evidence="8">
        <location>
            <begin position="134"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 28 C-terminal" evidence="7">
        <location>
            <begin position="332"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="34"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="533" mass="58042" checksum="3A1BD704E6F798E7" modified="2025-10-08" version="1">MRTLLPQDNDVSELPPVLPAFLLQRRTSAITIRPRAELDPSAEDLSSRRCSNDSSSTSGVGQNKSSKNVDLRFSFPTMLGGRFSLGLGGGLYLGGTGEAKARSLWRFFCRRHRVVKDADRKRILIGMSNTGGGHKASAEAIQAAFQERYGNKYEIFIVDLWKEHTPAPFNSMPDTYSFLVKNAFLWRFTYEVTNPKLIHVPYLSAVGSFVSRGVHEALDKYHPDLVVSVHPLMQHIPIRVMTERVKSGAMPPTNFATVVTDFTTCHNTWFCPGATRCFVPTEYCRQLALGNGMAPQQIILHGLPIRPSFSRPLSSRPVLRRSLGMDTGLPAVLLVGGGEGMGALEETVSQLDARVGDKCQVVVICGRNRSLQERLRARPQGPGHVLLHVCGFVDNIHEWMGACDTIITKAGPGTIAEALISGIPILLNGNVPCQEEGNIPYVIDNRVGAFETRPDRIASIIASWLIEGGEEQRAEFTAMGRRAKALGRPKAVYRIVDDLAALADEPCFDFGPAAAALLGQRNGAAATAVAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXX6</accession>
    <name>A0ABQ5RXX6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">B9 domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004884</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010796">
        <property type="entry name" value="C2_B9-type_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12968">
        <property type="entry name" value="B9 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12968:SF2">
        <property type="entry name" value="B9 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07162">
        <property type="entry name" value="B9-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51381">
        <property type="entry name" value="C2_B9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="438"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="540"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="572"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="683" mass="72937" checksum="D1D7CB9A305928B6" modified="2025-10-08" version="1">MTSPQRLAPDLATMLPGPRFQPSELSNPVPAQGLQRAPSSSPPSLRMDEQTVAEQRGARMERRQMKQASPLKGRNVRHSGTGEPDNGLASPTRSLPQSQSQVQPLGTPPPIRTASPEINADMTRYGLVADMTADTASPTVRPPRQQLSNPERQSPEQRSAVTPTASDQGSELESPSRRISRTDLYSSRDSLESLPSMSRANRKGSGFKRGDKSTGGVAAAAAKAPSKAGTGEMHVVGEIIGASGFSFPMLFCRWELVYDPSKSWKVLRGLAKGSTHACCRGWEEEELVVWDHPLDIHLQTQSLQGWPSLLLRVHARDESNNRDSFVSYGLVKLPTTPGLHHVSCRTWFAVETDRAAGRNFFGWHTGLIPRLEDESFVTDLRQREDAGPFICTVGTGEVHLRMNILTKNFSDITHRGGESLAAALERLNANIQKSVFMEERRRSLSDPPSDGQRLVRRGREERMASARSALDSRRRGDQLRSPAPSETSRNPSFLERNSRSSFRGFGGPRASASPGRLGLDPAGAQEFDGRSETSVGSFSRPRDRYAERLARREREKAAREGGSATPSVIGGEASGGIGGTGVAPSSDSGGAAALSRPVSISGAPASRTNSSIVEQGGRGGAGGGGAAAVAVTPGRQTDDDDGLETVMVEDGGGGAATAIGSDDLAAARAAARTARRAQPRSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY31</accession>
    <name>A0ABQ5RY31_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="859"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1134" mass="114424" checksum="9DE8C416254142FE" modified="2025-10-08" version="1">MEPQAIQNKQINTYMVDPYWRGFAWTSRSSLYSEGPAFLFDIMSVDQAGGAGGSFFAQSSKAGAQTLGDENRSSGNLSITTIVDLLASNASSGGLLKPLPTAKVAPIPDSSSPAVLHTQGDLIAVKSEDADAKLAVPTLEASVKAAPSQLYAAGKRTSSQAFSGDAAATQIHSTLPRRPRWLAALPVGLGEDVLSYHSHGTGSQQTYEVPSFLHAEGQANTEFQPSLEVLEQLQEVMGATIIESQSNGGTRERCPEFTSTLPAATATISSVLSGLTTGSSGKLSGFLGGTSPAVLEQLIAAALVGRTAVSASAPASGQAQERLAAASIYSPESVDGVTMQRKSFGSKHVHREESQFAEPGPTRRVQGDPDASLDLSSRVSASTVKGQERFGERTTANVSGAARGSASGSADDVSLTMPASDQAARRGSGLSEEAGCGSAKDDDGRGCGKGIESDVSKLAGPGRSGKSNLVPSDSTGRVRPASAAVLQPSLVWDLTARATVGTLAVTAPMLVGDSASGPAAQRTSAAPSDPASVQTSEPVSVQLAAAIPPELAARLKKPVRIIQPISQTWVEETLQCASGGTGPPSSTSKAKAAASLGPTSSTGKHCPPTSKVESALAPTVGHCCGAGMTTSPTAGSLPPSGAVTVVREPGPSERVGTAPQSSVPAAARAMPTHLSALRGTSTRATRLAVVTVGTQAQQTAPQPSWNALKPQTLVVGPSAGEQPQQLAALAPALTTSLPLADNISPFTASSTAHLLPQQQQAASAPTGASAVAAVLLPAYDSNVTAVSATWTVGMSPFTFSFGPGCSIDAAVAEAGPAAVDAGVGETSTAALYEGARPLALESDIPSSSGRTTAALLSGAVPASPQEPARDVAPSQPDVLRGPLNPKGLVAAQRTQQAPPLPSPPQRQLSAATGTTSASCLGKVQGELTLLYEWYTANKAVYLDQAQRQLQRIELLTAAHEGGTGNGTAAAASAKSESGEAMRAFETALKIMSVCGRVEQQWLSAAGSAGASADSRQLQLQVAAPPLRRQGFVQQVADADVPGTPLAADMALDGSAMDLAAHGTSAASALLPSTGWAHGASAALAVAAALVGSVSQGQRPARGYSGMVPGSLAVRPTGAGTFPQAAGGAPSRGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY79</accession>
    <name>A0ABQ5RY79_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004593</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="39043" checksum="86D1A3FFEE04DD4A" modified="2025-10-08" version="1" fragment="single">PKEVCATSRVSASMIKRRLLVGILGIGSVATGATLLWAWPTYNSLPSKHEPISPEQCSLIRCVPDGRPVDPVHNPGARVFPRFLDDTEPLLKELQPIKRDFGINLIEPVHAAIYRWQMSYLSRPPTVNMLRVTGRPEHSQQRRPWGYGDNFDESALPPVLKATADRIHAIPGIRLGRLRDVTINWRHSSFLRLDPHLDPAGDGENIFILSVDAPAVLTLSPNNWYRLSRWLSLLSFEDERDQVRREAERSWTSYDIDVLLPPGALLHLSGDARWKWTHGTRLGVEAPPGAAMPVQGRSGGGGDGGAGAAGDTGGLVAVRGGGRSGGGLLHWFGRPGELFGPTLERHSVVFAFGEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZC3</accession>
    <name>A0ABQ5RZC3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="171" mass="17860" checksum="47812D86D0618FF4" modified="2025-10-08" version="1" fragment="single">LPLAFLTRLAPLASVTDRSLSHDHAAAAAAAAAAPGSSTVVGDHHQQAQPRTRQVAATSTRAATGGGGGEGNTVLRRLALYGTDSCSGSTDSDLRCMRALRELVVGEAKAPAVAGLMRASSSLPYLTRFEALRYKRGRIFPALQGPMGPALAGFTRLRSLRLHSARVTQWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZG1</accession>
    <name>A0ABQ5RZG1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="290" mass="30442" checksum="50AB370E56A5ADF9" modified="2025-10-08" version="1">MYSPTLPTPPKSSSAFRVRPSNSLHAQPRRRLAMNRNKSPYVSDLSLTCKSGLPICSGSGSMETFTRAGSDRVLDAVRKAVGRVPDGLLDASAFQQQSGSLNTMSESSPGGNGMGGHGAAGLGGGQDADGCHEDDPYAAIRPDGGHAMRWWTYAFAAILAVGGLLTTIWTGLLEPLQLGWAAAAMLVLSGAAMSRIDSAPGALGVKLAWAVCAVIVLKESARSRLLNSPGWSALGLTALATCMGYMLTDMSALDEVALPTNPGSVFKSPDIADRSRVWQEWGYGQVQMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZM1</accession>
    <name>A0ABQ5RZM1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">VA_PAR_021</name>
        <name evidence="2" type="ORF">VaNZ11_005775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025461">
        <property type="entry name" value="ABA4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34543">
        <property type="entry name" value="PROTEIN ABA DEFICIENT 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34543:SF1">
        <property type="entry name" value="PROTEIN ABA DEFICIENT 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14108">
        <property type="entry name" value="ABA4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="244" mass="24982" checksum="4A8ED600E30E743B" modified="2025-10-08" version="1">MATSLRSCLGKSTPGLSTAWRKQQTIAIIPELSSNADVRGAVAAAAGPRRLAIRAAPAAASTAAAATCKGCCCGASASVAAAGVFGVSAATWFTASSALMVPMYGLMAFFPTLRLTRDLLFNSCALYVGLSLLYATTLVGAWQAGLGTALAGVVSSVHSAVLAGCAVTAIDLSPLAAMFSQPLVTLLSWLHLLALDALMAREIALDGIRTSNPTAHSVLLCFFFGPMGLLSHALTRLLWGKVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZT0</accession>
    <name>A0ABQ5RZT0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_005206</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011294">
        <property type="entry name" value="3-OHbutyrate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01963">
        <property type="entry name" value="PHB_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009093">
        <property type="entry name" value="PRK12429.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF2">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="266" mass="28020" checksum="7B235210F115265D" modified="2025-10-08" version="1">MAANAILKGKAALVTGSTQGIGLAMLKALASAGCNITMHGIPSDVDKIQGLCKQIAKDCGVEVTYSDANLLNPGQIRDMVRATHSAFGRLDILVNNAGIQYVAPVQDMPDEKWDAIVGVCLSATFHATKAALPYMIEQGWGRIINTGSMHALVASPFKSAYNAAKHGVAGFTKTVALEVATKNITVNAICPGYTDTDLVRNQVADTARIRGIPIESVIRDVMLADQPTKNFVKPEDIGALVVHLCGPNSDSFTGACLSIDGGWVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S012</accession>
    <name>A0ABQ5S012_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045591691" description="Glycosyltransferase 2-like domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="49821" checksum="32FA7C55F40B9410" modified="2025-10-08" version="1">MLMSCPRNRKLRLVCWTWLMCTVFAGTSAGPDGNPFPSCKVFQTYLNAYARQTVGYLVQGIQARDASLGSLRLGRLYASANATPSEASLRSSKDAMYSQLRDLLLAAGIPSQAGLSGIDAAVALAMSCMSSQLLPASYIGRPVVSIMLNYFKRRHVVKEIAENMHAACLSVSISCELVVNVDNPEEAGAWANEAGFVVPVFSANLHESRGYNRAARLARGKYLVVWQDDQIPPRTGIWLLQMVRLFETYPRLGILGMNTYRICRQKEITNRWGQPGWDPDPRTGITWSFVHFADFAPMAIVASIFWELGGLEEGFSRPGDCGITGDWELCARAWVAGWQVGHFSWEGRKGDPEPGSTHTSVGAVACWNRQMDVGGGSFTKRYIHPVFVQDMCERVWAHNMLTFTLPSPDRCPYGEQTRGWANCTAPPAGQRTAFAAQMQVQLPFELDPTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S066</accession>
    <name>A0ABQ5S066_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005558</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046182312" description="Glycosyltransferase 2-like domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="59773" checksum="875F1E1807E6A55E" modified="2025-10-08" version="1">MGPFIRLPQRFLLELVLAIATACVSVLGGPDHAALSSCHDLNKGLQPEQRHAVRDVIQDLQAIESYLGHLRLGRPLPLSSVPAAVPSVAVLEEWKVQNTTKLQNLLDSGGVSGRLGISAEAAHVLAIICMSSRLRPDGYTGRPVVSIMLNYFKRPQVVSRLAANMRASCASVSVPCELVVNVDNPEEAGAWANEAGFVVPVFSANLHESRGYNRAARLARGKYLVVWQDDQVPPANGQWLVDMIKIFDSHPQLGILGMNCYRLCKHHEPTNRWGWSHWAPDSATGVKWTYAQTVDFAPMAIRASIYDAVGGLDEGTSRPGDCGIWGDWELSTRVWMDGWQVGYMFLEGRTGDGQPGTTHKGANGEKCWGRQQHVAMNVYARRYGARFLQDPMCERVWLLNTLTFKVALNGKCPYGSEETRWGNCTQPPPEVAAAATAEFANLASAAAARSRIGVRSGGSDHSTAESKRLAAAAIAATMGSQWIAALGASVGKLLPPPPPVGTSTGETDGAGSQAAGDQVVPKADGLLGIMDIDPMLVRTAGSSGSSGGGRRSIRHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S072</accession>
    <name>A0ABQ5S072_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphotransferase</fullName>
            <ecNumber evidence="11">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_005336</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the hexokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="11"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="13">
        <location>
            <begin position="46"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="14">
        <location>
            <begin position="277"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="173"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI62661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="557" mass="59577" checksum="E8EDE19AA6605A0C" modified="2025-10-08" version="1" fragment="single">VVRLTMRLAFALPVIGTGAVAVALALRKCEPPLTSEDDNKINDLLKHYKSRLLASPERLAEVEAEFLTQMREGLSKRVIVDGKRMMMLPTFVTRLPDGTETGECYALDLGGTNFRVMHVRLGPRKGEVTACDVEEVTLPKEIYTGPGDKLFDYLATTLKSFIDCHRAAAATATAATSVPPSERTSSPSQQPQNSQDQPPLPVLGFCFSFAVEQDGLASGKLLAWTKGFSCPGVVGNDPCALLSAALERVGRPCRVAALLNDTVGVLAAQRYLDSDTEIGVIIGTGTNACYVERLAALSKWEPPTGHEASGDGRTAVNMEWGAFFSPHLPRCMEDLQVDAASPHPGKYLFEKLLSGMFLGEAARRVLVTIAKEVEGLFQARPNDVHALLADARDSSSGNMLLEVAGATIPKPLATPGSLSSATLSAIVHDCSWGLRKTRRLLVSELGMHQPSTRAAAVVKEICTLVAKRSAQLTAMAILAILRYNGWADASPPRPVTIAIDGGVYERFTAYRTMLSKAVSEQLGERSAKLFPLIKFVLSHDGSALGAAVLAAAAVTGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0H6</accession>
    <name>A0ABQ5S0H6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006113</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12307">
        <property type="entry name" value="RRM_NIFK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46754">
        <property type="entry name" value="MKI67 FHA DOMAIN-INTERACTING NUCLEOLAR PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="28"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="185" mass="21582" checksum="4C1717E0F8895AA7" modified="2025-10-08" version="1">MPKTKQPEAEMDDASPSGGTEDDLTMARVMYIGHVPHGFFEKQMLGYFSQFGKVTRVRLSRNKKTGHSRGYAFVEFQYPEVARVAAEAMDGYFLFKQRLSCKLMPPNKVHPQLFQGANRKFKVIPWRRVERLRHDRERTPAEHAQRVVKLIRKDVKRQKKIKEAGIDFEYESLSIPVKPKKVVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0R2</accession>
    <name>A0ABQ5S0R2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="178" mass="20095" checksum="E5947555EA86D2DC" modified="2025-10-08" version="1" fragment="single">LIPGLDPQTRFRPVPKVTGVAGYWAKIPELSAPIPMPIDVALNANRVARVAHESIKGILLRETDTQIIVKARLTLPLGLKHTHEERYNKDNSTYTLMIRRDVRPGRSITRMFYTADGDVLLVCESLDMAGRTTHIGYELAHVYENGEKIRCRQWVLILHSGIMVEQYFVGCRAPLPGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0T9</accession>
    <name>A0ABQ5S0T9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 3 subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_006266</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007783">
        <property type="entry name" value="eIF3d"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12399">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12399:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05091">
        <property type="entry name" value="eIF-3_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016281">
        <property type="entry name" value="EIF-3_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="90"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI63344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="61817" checksum="10DC485D35D8B34F" modified="2025-10-08" version="1">MAAPFPVPAVTDNPDGWGPCTVPAHLKDIPFAPFNKGDKLGRVADWTQGTFGQKYPGRGFQQGGIPVFNFFANEEEESFHLVDNRPVKTTKFGASRRYQQNQRFQQQRRDGKDGRGGPEVDKKKGGAQQQKKQQYYGRDNQRQTQYSSSVEIGADWQVMEQITTTSLSRLNYSPGEPEDLLACGSLEYYDKSYDNLAVKKEKPLEKTKRIFRTVTTSDDPIMQQLAASDKARVFAVDSILTHLMCMQSSKYGWDLLATRKGNKLFFDWRSSSKFTLLTVNETAPEAVPEDKDNINGVHHLALEATAINQNFSQQVLIKDGGKLDFGQPSPFASPSDAGMLASCAYKYRKWRLASGSDAPEIVVRCEVDAALRTAEGTAPPTVLIKALNEYDMKSQDYRKRLENQKGAVLLSETKNNKFKVAKWTASALLSGADQIKLGFVSRATPRDVSNHVVLAMHGLKPRDAARSINLEMDNCWGIVRALCDMLLALDEGTYLLVKDPNKELLRVYSVPSDAFAAVSAPAEDDDLDGVDMGGEGGVVPVDVTVPILPNISAVAADVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1K8</accession>
    <name>A0ABQ5S1K8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="212" mass="23517" checksum="E8663CFC89D9834A" modified="2025-10-08" version="1" fragment="single">MEPRRYVVSPDSTPPATPATTSVPAVTVTAPVTALVTAPVAAPLTRGNVPVQRAPAQQWHRDSRAAGGAPLPQRAWLGYWQEPGHGGGWWQWPVHGWFQKPGHYGWLQKPGHYGWLQKPGYGWLQEPGYGWLQEPGYDDWSPEPGYCGWSLEPGHDGWLQGGIWPTYVPPPHRLPQLKSRGSRPQPPPPQRQPPPQQQPPPPPPPQRQPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2A4</accession>
    <name>A0ABQ5S2A4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="786"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="899"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="742"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="786"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="938"/>
            <end position="957"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1018" mass="105775" checksum="D3F3A131ACE8B4C9" modified="2025-10-08" version="1">MDKVQSPDSRFNFVFAGSTSTTEAADNLDAALCPGMVAMVDAFTPHSAYSPGLCFHLGSRDQGTGSTRCTGRGHGGVGRNYRTTELHRRFLPGNCLQWNSMVIQPGPILATSPSLGHIDSISALTAEDSSGNALDKLISLLLLPDGDNPQVDGSNASAEPLLSSVSFTGVAMNNSPEADRARTTSILGSNAVANGPVESCNSLGRDRMFAGTHPDGKLQLPWLRASVGAAWGRQVCGQPVSSADGKGAGGHSPGVSSGKEDGAMLGLHALAAALDEVEASLDLPGATEAAAEEEKDHGWLPTGLFSQRQAYITSAAFEGQHQQTLRRLRRPKRVARRAVVPSVVSSGQESPLARSQQDQNVSLAGANADDSWRIMVSAPMPHNPEALATLLISGRLINPHTAMPAPLLAAAARAQQQQRQNQPKNRSCGGEDATYQSPSAPQKQQDGYLAAGGKGPAVALVRCASEHDMAAALALNGRRLYSPSMTRNKADSWRPLTVLVQAWAPAASEGGQTGMPPAMAVPVSISSCSPQGSRIRDTGPRLRRVVLTGLPGHMDVDDVIALFGNGLLLPGHCPTPQDRHFLLLCPDAGALSALMEWSGTRLPDKPLDALKQIIPRVNSWSTIRVEEDDGIQLTALMSQLMVSARVCNSTADNIAATAAIRRHQHYPSVAGRFHPAELDEMDEMATAALEAEGRLSGSGSGNLTARQSVKELTSEAVSSREGLCGGAVVGVSPSSLDKPRPHVAGAAAPAGPRAGHEPMKAMPSLDRSLEVPTGMLSLDSVHDAAAAAERAESSRGASSSGLATRPAPRFRRATHSASSQFSTSSSAAGAYFGQGISAGHGFTGATAAAAVAARCACCPPPSFAGRSGVGGGDAGPTSWSGQAAVELLNKLLALGIEPAAGSSPQGAGHDGSGVTRETEDRMPFFRRNRKRMPVYPLPDDEDEAEEEGSGEMIEQEADAGPGGDCRLSQAEPAKVEPAETAAADTGSGEEVWHTSTTDPVAGEVHGLQAEDVTPNELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2W7</accession>
    <name>A0ABQ5S2W7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_006705</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045869313" description="Peptidase C1A papain C-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="45"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="4">
        <location>
            <begin position="215"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="439"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="684"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="684"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="703"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="718" mass="76755" checksum="618B4780F0E534A6" modified="2025-10-08" version="1">MVRKRTWPCCPYSCGEARNTIMTTFRSLLLLSAIAMVSLGTASAAGCQVVRGYDFQPQVEVTNAFRIKNFTVPSNLTILGDLCANNVSRCNAFNTRGWLMTAPLRPVFSYLDNTVTSACDGVYIANRTLTGLNLTTRTVDELRAASLTINMVAAVAAASRVTIALKSASVPITPSNILSQTKAVTTAFQMTQTPLAQVSNNTGNYTFTAVANAILYPAWDSRVAYGSKNFISAIKDQGNCGSCVAFATTAGAEAAVAAAYNSTTNTNDFSEQWLFFCNGKYKPTCDTGWDANQASDVLKLYGIPSEANYPYKGKSVPCTLTGTLTKPDGKFDWVQITDLDKAKEYIRLYGSVLTYFAVYNDFYNWGASSPPYVWDGKSALAGYHQVLCVGYNDAGRYWIAKNSWGSSWGDNGFFRISYTNRAGFMTASGDNVVGLTFTPNLRPQPIFSSPPPKPPPSPPPSPPPVKSPPPSLSPRPPSPRPPSPAPFSPPPRPPPPSPPPPRPSPPSPPKPACGDKMCNGVETCADCPGDCGACPVCGDGVCSGGETCRSCFKDCFKLLNGVKTCCGDKVCSPGDNETCSTCPLDCGVCPLCNNNGICESVRYNETCYNTVTKKGCLDCGTCGAATCGDGICTFSTNSYSESCTGCSKDCGKCTDDFYCGDGICSSSIGENASKCSRDCLKPRAMPPSPAPPSFSDAPPPYNPYDYGDYDYNYYSQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2Z2</accession>
    <name>A0ABQ5S2Z2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="179" mass="19344" checksum="EADDED9FD02B4F5B" modified="2025-10-08" version="1">MGETCLHVPARVFGFESTEEIASQPQRSPQLEQRASLLSALLKGSRQPLPSAYMDTSDVDSDDEDEEMEDGEAEENDGVAEEELLSAAGGSAPVPIPSTSETFLKAQREALKRFVYAHQIEEMRKQCAVYFARQARFNEVTAPDSTVSCNQPLPGAAVEALSQGAATTQSTAKHRTPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3E6</accession>
    <name>A0ABQ5S3E6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_007647</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF254">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN SHAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="459"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="14">
        <location>
            <begin position="289"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="674"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="774"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="834"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="901"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1040"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1081"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1341"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="680"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="706"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="774"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="839"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="901"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="915"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="963"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="994"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1041"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1081"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1102"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI64381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1373" mass="144635" checksum="E88DDCFDFB32AEB1" modified="2025-10-08" version="1" fragment="single">MNGVSRQNSMAERDPLRPVLQRNISGSSTPNCVREDPKLDALIVDAAPRDGDGGNGVSQQPVACPGAVDSPKLESPRKQDSDYLPGSNDPRPPSAGASPSRPSLAATPLPASPQRSVVLLTPPQSRPGTAGPWGKESMVPPGGTSTGRAGSPPSTAGSGPAPKPSYRALSRAYSRRGLPRSQSRASGTAGSAKGAADAVSDTQVVPFNQNWVSLRREAEDRAKIAGLLSPETRYWVHVDKYGNLLPAGRKAEEGDEDIDPNSWRSSIKRYREELWNLMTDSASSKKAYAISIVVILTIILSTVCFCLETVPEYSLNPDVHHAFQFVELVTVQIFATDYILRLISCPALIRFLISPMNIIDLVSIVPWYVELALANSGLKGTAVFRVLRLLRVFRVLKLGARYRKLLLVTSTLANSVDMLMLMVFFVSVVIVAASTLMFFAERGTYKDELHYYVSPFESIPSGFWWAIVTLMTVGYGDVVPITMAGRFIACVTMLCGILSIALPVAVISSNFTGLWEAYQRQQKRLAVGQGEASLHLMDLAETLGRHLDSIKDLDNILEQTLSKMSGLQTKLRERGVAREAELESLLQQWADVVTPPQAAAARRLVSALSRLGTDPEMEARRAAEAARLAALEESLAALQYEQHQQLVAMLLPLPETDARGTLLQVLRAAAPDVTAAAAAAPPPPPPPPPGQAVSEGTENGVMTARWDQEGRRMEDREPQKLSPEYDTGAGVRSANAAREEKALGMGGRGGFGASGTAAGELLRRPLPLPLPAQTDQQLQSQQLHLRASPPPSREAVRSLVARLQAATEFEEEESVVPQAVSESAEASVGAWERNQLEGQQPQPQPQTATAIAAHHQDQQRAGSGTEGRGSGGAVAAATAGESPLATAGAAAAAATVTTGNQPHLQVPQGANQEPPSTPPLPPLPSQLQQGQEHPTPAAAAAAVEGAQGRQSIQVVDDDGIGGTAAAGRNSSSSSSSSSSNGEAQRHGNVHLTTPPRPRTPPSLPTASYDLPPPLLPELPAVTTVPLHAAAKIAFNVPPTTAAASSSSSKSGNAGHVPRLHNGGNLPAGVLMLRALSARGGRAGSAAASGRPTTIPGNAVAPHPLPPPPPLPPPPTAASVNIYPPQQQQQQLLPTSVPSAVTPFSAAPGGTAAAAAAAAGPAPAGAVPGAALKLGSWGSDPAADAAADAGDEVVGHQQWRMDETEPIASSSTAAELREALGELRDSPLHERMLEEVGEAVALEEDLHSCWRRLAAVLRACSLMCGDCLPENLDLCRSQFRQIVQLDKETRELQGRLDIVHQDIRELQELSKSGIAVVLTMGSELILRASHQLALSLSIRPKPKSTVTTVNDPDGANSRPSSSRRSRTPGGEGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3P2</accession>
    <name>A0ABQ5S3P2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NTF2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007293</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039539">
        <property type="entry name" value="Ras_GTPase_bind_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693:SF20">
        <property type="entry name" value="AT27578P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="NTF2" evidence="3">
        <location>
            <begin position="10"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="250"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="473"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="487"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="557" mass="56971" checksum="9E5E127947FCF386" modified="2025-10-08" version="1">MAQPPSPAWVGEQFISKYYDVLAKLPKHLHRFYKENSTFSVADVQPGGHAVVGTVSGNLEDIQEKVMSTIANAQVASDMSLDAQFSQGNGVLLQVSGTMNLQGVDRKFVQVFFLATQEKGYYVLNDMLRIFPPEPVRDMRPVENGFVAPAIAAPTYGLAPSQLPAQLQPRVDIAPAMAPVLAVSTPSVIAQSLPATEAEALVQEKPAAALATVFGPATAPAEVPTPAPPAVTVLDPAVSPPAPPAVAQSAAPALAAAAHPAPTAPKQMAPQQTTSSGSVGSASSAPLAPPPPANLSWAERARLASAAPVAPASASKPVTPAKAPPPALPVSLPEVPAMEATVLPAASSALAPSDTDAPAGSQGDADVETRAPEVLEPVTAAAGNDPGFGVYVQGIPQTGDLKKLLSDEFAQFGPFGPGGVNVIRSPRFGMVAYIFYQDEKSRNAAREASGKLRLLDSDKPVKILGMLPEFERGFAPRGGGRIERGRGSGMGSGYRGGRGGFDGNRGRGGRSESGRGERQMGGPGGRGGRGGDRGGRGGGSGRGPRDANSGPAGPKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4V7</accession>
    <name>A0ABQ5S4V7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007832</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="217"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="280" mass="29752" checksum="9582B3D4BE4DE9D0" modified="2025-10-08" version="1">MPSSYRRAGGANRSISMRELMEMPFDQLANSVEAATTVSSSRISSIRVLPLLRGPAFGGSNGGSGDVPGGPLLRSHSALPSVLQFGSRGMEEGGLMDLVHMSLRDTAYCHSPFGGDEAGGAGSRSSPRAPHTAADRGGRLGRLPLALAVALDNRAQTSHGRPYTTGAASEGGRPGLPYNYEQLLALDEERMRRVVRPEVVRALPMRIAHRSDGAQQCHVCLEKYTPGRTAITILPCTHAFCSDCIRPWLAAHSTCPVCRWAFPERQTVLVAGEAGGGAHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4Y9</accession>
    <name>A0ABQ5S4Y9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="562" mass="58598" checksum="087AE6601D5C8846" modified="2025-10-08" version="1">MAAHPGSGGGDSVEYVYGSAPPSPTGTKKKPSSPNSSSSRLGRTDGWVVPTPSPGPGAYNPSVAMVVSASERAVFGTAYREQEEKRFMSALHNVGMPNTETPGPGAYRTADNRLLKSEKSAKFGTAPQRPDVPKESLCKPGAVYNLPSTLSTQTVSFGTPTNVVRASLARLNSPLRDPRSMQSERFPLEHIEGPGPDAYPLVAPEVTSQRETAPMYSFGRASRESATRAHLTQQQLAADSTVSTAAQTPAAVYDIPSMPPPSQVTVFPRGPAHYNPEKGGNAKGPYIGRLHEDAHKGIDSPGPGAYPAATSLQTAGGAKFGPSAPSRTPKPNEVEVPGPGAHNPNFGALSVRPTASSFSMGANLRTDWAAFSPNTPGPAAYSLPSPDRTNNNSIAPSFSMGAAPGGRLSHVARSNTPAPDAYGRPEDPRDRNSVKKGFSFGLKERCKQEGTVISNRYHGPLHAQEGMCTESPGPGCYAIPPSTAAVTNTNGTRFGTSSREAGSKMYISHIHAAAEGFGTASPGPGCYNVAEAGGLGSPAKFKAKVAPKFGTSPRFSDKVCTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S540</accession>
    <name>A0ABQ5S540_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">KOW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007652</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_B">
        <property type="entry name" value="Ribosomal_uL24_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_uL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057264">
        <property type="entry name" value="Ribosomal_uL24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01079">
        <property type="entry name" value="rplX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17136">
        <property type="entry name" value="ribosomal_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="6">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="177"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="248" mass="26908" checksum="5EC0AEB46569BC4C" modified="2025-10-08" version="1" fragment="single">MPQLLRAIRPMFKTDRWRICRDDVVYILSGPDKGKTGKVLEVVKDQRVPRVIVEGRNLRKKKIRTGPGDDDYFVVTMEAPLHYSQVQVADPETGRPTRVVFMFTPDGKRVRARKVPNPTINDIIPTPPEDPDPRAGLVGAKDTPAQLARQGTYLPSAGFPFRVRNLERIWAGLHPDVPSSPPPITEGDNEKGRVGRVGGGGSRVLFPSALPSSQSPLSAGSGIRGLCTATSSSSSSKLAATVHSELGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5U9</accession>
    <name>A0ABQ5S5U9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 115</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_008116</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013861">
        <property type="entry name" value="TMEM115/Pdh1/Rbl19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377">
        <property type="entry name" value="PLACENTAL PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08551">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01160">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="34679" checksum="2DBEF8C4F6262C3E" modified="2025-10-08" version="1">MDFKITRLSKVIAVLLVVLYFVAYVFPQTVDYIALIPGRTLPYVWNLFTAGFLTTNPIKLGLEVIAVLLLTRLVEPVYGSKEFLKFLFVVDFSVSLCVLAGVYIIFAMGKDTGDILYTKFTGFHGILAGMVVAVKQVMPEHEAKLFGFVKFTFKYLPVLFLAVTVGLAAALKQFSEIPFLLLGTYNAWLYLRFFQQQPDSQHWGDSSDDFKFSGFFPGFLAPVIDPIGYVFSIVFRLRHPPAETKAPFAKAAQYTLALPADNADANRRRERGAKALEERLGMKKAAGEGEDVESAAISAPSNTVVIPTSAPSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S690</accession>
    <name>A0ABQ5S690_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727:SF2">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="28187" checksum="4E22F26EC3D40152" modified="2025-10-08" version="1" fragment="single">GRVVASSFRTYYRPLNQQADFIEALRQSRAFAARASRELKLDVYAYSLFHVFFEQYLSVLYDTAAMVGLPLLAVVCVAWSLTGSLWSSVLLAGVLASLLVHLGGAMLMAGIQVNAVSLVNLAMALGIAVEFCAHIMHAYMVAPPPPPPPTTTTTTTATTAQDITAPTAAETAAQSVAAAAAAAASSRVSRSRAALVSVGSSVLSGVTLTKLVGVAVLAFAHTQIFEVYYFRLYLALVVLGAAHGLVLLPVLLALVGPPAWQEAAVAPSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6D5</accession>
    <name>A0ABQ5S6D5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033246">
        <property type="entry name" value="BIN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34810">
        <property type="entry name" value="DNA-BINDING PROTEIN BIN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34810:SF1">
        <property type="entry name" value="DNA-BINDING PROTEIN BIN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="443" mass="45463" checksum="526A393FE3CAFEEA" modified="2025-10-08" version="1">MSESSGDTPDWLRQHTETPIKIFIASSSDDDDDIVLVRRNISVPSGFGGGPGPSQRAATAAQVGQDEGLQQSAQQPGDLAKATCQRPKRNADQQAAKDEETQPLPELSQQSALPESSQKQVERRQQQADAQLVAGVPSIAANIGGALEAGKLPIMLPEKLNRNKVLLELPAAPASGEHYHGAADLSGDSGAVGRIVIVGAGASGKGAAATGGLDGGALQIDLKGVMYNAWVLPLATTALVLNIGPTEAKVESLFNDFIRLREVVHPCLADEDRGDARLAYLLDGDDENYQVGGGDGDDASGQGAKISKGPGKPRKRAADKAAGGGPEKKAKTSKKAASSKADKKDGTGAGVGAKKAPAGAAKKKAKNELRTAREGKKSAGPKKTPEAKKAAAKTPAEKGKKTTTQGLGVQAGSVTKRASTGRKKQTTLRAFAAAAKPGSDSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6Y2</accession>
    <name>A0ABQ5S6Y2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045631108" evidence="1">
        <location>
            <begin position="27"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="357" mass="39008" checksum="EC488577F6F32B12" modified="2025-10-08" version="1">MKRSKATMLAAVVTVALALALKLSTAYEITACPRVDGYIAYADMGVTRSTEADMYDTLEEAKVACDDDSSCWSFNNNLEVYSVTDVALGAAPGVCTYVKNVCPTYPGYKLLPPGFVADLGDTAVVVQYNSDDDLPLECWNSDLDICAGFDITGRMIFPKQGYGVKDVVWKADNITTLCAYQTQDTAICPNKFGFFTHYDSILKPGQGLDGVNYGQLETLEDAEAYCKITPKCSGFSSDGSFTVGGIKGLSFAYYTCTYIKHPCPPVNGYTAVDGYTIKAFSPSASYPKLCFADLVRKCNDDPNCQSFDTLRNVWNIDVVQYHPFPGMCLYVKAQGFFGRISRRRALGEKVVSVVSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S720</accession>
    <name>A0ABQ5S720_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF444">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="355"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="540" mass="59889" checksum="E232A62D3C88C2FF" modified="2025-10-08" version="1" fragment="single">MSEQLAIYRRCRRQYPGTAACVLLPAFCRRKAEASLRGMRCVHRFKPGTVLFNGVDREGMLAPWPGTSYSVDVWYEAPDEGAVGYGPALSALTLSAGQSLVDNHVPVVQIGLNGRTLKALVDTGASDDFISVAEVNALGLTPESSDCPQVTLADGGKHPIVGRITLSLSIGPLRVVTRPYVLQGLAETAPYIMGSLTLRQYRTRIDMETATLHMCKGTLSIKVPFLSFEHVTPKEGEAEAVPMVNFATAMAARIEPELVGRKEAVRLLQKGVHGLLLRPRVGCCATTAQISVDPEVEALLSQYEDVFKDIPGLPPMRPVDHTIPLLPGAQPTARPMYRLSPLELDEVKRQVTDLLMKGMIRPSTSPYSAPILFVGKKDGTLRMCIDYRGLNVSTVKNRYPLPRVDDLLDKLKGSAYFSSIDLQQGYNQIHIAASDIPKTAFRTPFGHFEYTVLLFGLTNAPATFQAVMDRMFRPFIDRFVVCYLDDILVYSKTREEHLEHLRLVLDVMRREQLFAKRAKCFWAQPQVEYLVHIVSATGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S784</accession>
    <name>A0ABQ5S784_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5-formyltetrahydrofolate cyclo-ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017:SF0">
        <property type="entry name" value="METHENYLTETRAHYDROFOLATE SYNTHASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="434" mass="48020" checksum="96CC201AD76FB57A" modified="2025-10-08" version="1" fragment="single">HLQLQNLSLILSDRPTDYLSSSTLRHTYFLMHHLLARAQALVPLETSTAPSTSYRTACTLIRDNRLLVQRCNFLYRSKPKERTMLQAAQQPFDVNSYNTERLRLDEQARDGMRTQLARDEASERSLPGSVAGAWKWALRKQIWDFMEAKDIARFPRPVHHRIPNFVNADIAAQRLADLPEFRSAQVVKVNPDTPQKMVRQLVLTRDKVLLTPQPRLRTGFFSRLSRSSFPLEDLNEACTSAGVAKFGEPLSLDTKIKVDLIVVGSSCVDPTSGARLGKGEGFAELEYGILRWMGAIDENTLVVTTVHDCQVLEPGTIDVSKMLEHDVPVDVIVTPTQVIRTNTTLPKPSGILWHKLSPQKLGQIRVLQQLKERIEREMGSKLPTGPDETLPPSAARGKPPGGRGRGGGGSSRRTGSGSGSGRAQRGSGGRSGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7G6</accession>
    <name>A0ABQ5S7G6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40637">
        <property type="entry name" value="ESSS SUBUNIT OF NADH:UBIQUINONE OXIDOREDUCTASE (COMPLEX I) PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40637:SF1">
        <property type="entry name" value="ESSS SUBUNIT OF NADH:UBIQUINONE OXIDOREDUCTASE (COMPLEX I) PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="20118" checksum="7AFC75D4884D94CB" modified="2025-10-08" version="1">MSNQLRGLVRRVGAQLLRNPRVRGGGGEYPGGSFWSEGTQTGRNGFLFGELPPAPGQTRKALWWEPWWYIGYGGTALGIFFIYNAKPLEAFDIRYWANPRAAKELDVEMRMLDKLNERPDLKERLVAVCKDLNLIEEEAYDLVLMRNEYKVRLGLYTGRVPEELKSIYDELEATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7N5</accession>
    <name>A0ABQ5S7N5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_009573</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="527"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="569"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="598"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="660"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="689"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="6"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="361"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="432"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="393"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI65920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="757" mass="79497" checksum="32A4097C46F92B3B" modified="2025-10-08" version="1">MVLLWTCLFAALGGFLFGYDLGLIGSALLQIRRYFHVRSSGTVELIVGVAKLGAAVGTFLGGAAMARYGRQRALALNSVAFTAGPLIMAIASGPWMLVLGRFVVGLGVGISATVSPAYIAELAPARIRGMLVQLYEVMLCIGMLLAPLVDWALSRDDSTASWRVMVGLPALPGLLLAAAPLVLPESPRWLVTRNRLDEALQVLTLVTRRSGGLSLGLEGFWSGDYARMPDRDDQSVRDAEDELLQLWSGVQIDRNVVLEQRRKLWRLRQQRNGGRWGRLGCWMGHQGRPKPPSLEPIPEESSGSLLSSPSCLSTASSLAVITTAPAASSPRAPLGGREEQLRQVMLTSQCAAAAVGLVSSPGSPLRNQQQPAVGVGAGVGPGGAEGDSIGHGPASGGRTGGARGRLLSALSRRREGGGVWADDGAYRLLPSEEADAGRGDGGPGPDCRAESERSPPQHRPDERERLLLPGKAGSHGETVSEPGEAAGPSAPPASLADMPGEERSFLRTLTALLADISALAVGPERRALWLALGLAFFDQVTAATAIINYAPTLLKRIGGEDSSFEDGSAMLYTVLIGITKAVGVIAALLLVDCCGRRWLLVTGSLACSLALGGLTVAALAGSVDLLEVAMCTFTLFFSLSWAGVYWVVISELFSMSAKSAATSAAMCLLFLTGALTDMVFLSLHDVMGSYAFLLFAALCLASAAYVWLLLPETKGRTLAEVQALMVRDTGGGAAVAGGGGSSSSRWVQLWERRAELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7R6</accession>
    <name>A0ABQ5S7R6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_009126</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006189">
        <property type="entry name" value="CHASE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03924">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01079">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50839">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="CHASE" evidence="11">
        <location>
            <begin position="245"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="617"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="522"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="567"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="954"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="450"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="571"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1018"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1037"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1054"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="644"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI65600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1112" mass="118625" checksum="A59878573574941A" modified="2025-10-08" version="1">MAEGTDILNGHVAIDVPNGASGCGLCDGEAHQSAPLFIIHRDEEEIANIEQAACITVADAQPEPLGASETLDAGKLRSVVEKNSVAAYHDSHHPFSHIARRIRLRGGFGAARVGQCPDVTDKKRKGNVKRKALLLAVRRDKKVVIVPIFLLLLCMGLGLWGVFAASAQERRQRLSEAQNRAADKAQTIASELRACYLPVKVMQSFVTRYPYFPTLNATFASLATELLSLTAAGSIANMQLAPLGVVKAIEPLAGNEAALNHDLLADPKNRDFAIRTIQSHNLSLAGPYELVQGFKGAPARFPIFIHGVSGNETFGADRDVTNCSACYDPVTRTKFWGFATVLIHWNRFLYDVVHINDLVDEGLHYRLTRPDDVDKTKLFVLGGQNEALRDPVEVMITVPNNVWSLSVVDSRGWTPNWEWPLVAMVVVMSLLLSVLVGLALIGRTQQHLLLEEVLSANRQLEDAATAMLNEKERMEALLRRQYNLIECLGLDPGNSLNGNGGATNGADASAAALPGVASNGGGGGGGGGADGAAAAVMGRGPPTIGELHPSPADRIEEMRKMLLSGNRLGAAGKGNSGKLRGGISGDGGGDGGGDGSGRVHNSRGRKSANDGGSSTDLILGEMIGEGTFGKVYRGLWRGTEVAIKTIILPTNMSGKEKREKMAVMEAAISSSLAHPNVVATYTYQIKSLKDSSAQKVGRPSDDITASAIIVGNDKATDAGGSCQRLADPLAPFKEDIDEAQIRGGGIHSYEVQLVLEYCDKGCLREALDAGIFFGQSGLNFPAILDTAADVAKALLHLHLNDVLHGDLKADNVMLKSCGGEGRGIVAKVADFGLAIKMDHRKTHVSATFQGTLTHMAPEVLLHGQMSKAADVFAFGVTMWEMFTGGLPYQGVPGALLGHLISKEGKRPVFPPGTPAAYKELAEQCWHEDVAQRPGFDEILATLTALRGQQPCPTPAVSYSAMTPEQRQAAKLKEQEGAAASVRRRLLEEESARQSASAPLPRLHNHYQNWPPAPGSGSGMVNSQSPDLCTSRPFRPQAASSDGSQTRSLQSPRCRSSRRTGSRSRSKADEPIIMVGTRNVSLSNAITSKSAVLEPISETRFEGDYDAPAQDVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7U5</accession>
    <name>A0ABQ5S7U5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_009669</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14010">
        <property type="entry name" value="STKc_ULK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056980">
        <property type="entry name" value="ARM_RUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056981">
        <property type="entry name" value="HEAT_ULK4_RUNKEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044591">
        <property type="entry name" value="RUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46562">
        <property type="entry name" value="SERINE/THREONINE-KINASE ULK4-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46562:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ULK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24970">
        <property type="entry name" value="ARM_RUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23606">
        <property type="entry name" value="HEAT_ULK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="4"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="5">
        <location>
            <begin position="655"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="358"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="421"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI65982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1421" mass="151190" checksum="6AC23BC77366CFD4" modified="2025-10-08" version="1">MNNYHIYEKIGHGKHSTVYKGRKKKTISYYAIKSVDKTQKARVLQEVRTMHALDHRNILKFYAWYETTNHLWLILEYCVGGDLMSLLRQDVRLPESSVHDFARDLVTALQYLHSKEIIYCDLKPSNILLDENGRMKLGGFGLSRRLADINKKPLQALPQAMRGTPCYMAPELFSEGATHSTASDLWAVGCVLYECSMGRPPFLNSSFNQLVHEILNNEPQPIPGASEAYHDMITRLLDKNPATRIRWKDLCAHRFWQVKLPQLELPPEPALDAFIARYGLAPTVEEMRQSTRDGLKLDKGRTMRQSVDITRLSRIALHNLEREGDGADYSSTVASAAAVEAQQGDIRIDSADAELDFEENREEAVEEENSVSPAPSDDGMVIDQGSGAPLSDSARMISATANDAMMARAAAAAAAHQSSHAGLGAQAPARRGGGGPSESISGGIGASASTVGRVPGRNGDDLRRSTAVGNGRPSDVATDIDAAQGATASSVGVAMDDDLLVETGPLVTDRDAASLDDLIWHASDTAVKPIVANRRIERLPEPRYDAASLPFRPWSLAEMLSANQADLEGFLTHIYRAIASAAPLKDKVNVLSYFETLCVDTNAANVLINSSLTILFIRMLRNARAPTLRIRLASVLGLLVRHATYISEELANTGVVEVLSEALKDKNERVRRRVIATLGELLFYIATQQQDNAAAGANGSAAPADPAVVWGISGNTYAQVIRLLKDKDDEVAAHYAVKTVENICSQGGEWAARFATQDVVLSLVQLINNTGKGEQLRATAASTLARLLRHSPVLVAHTVDKYGIRLFVGCLLEGTVSASASAGGTGGGNGGAGGASSKVQTAALNLLNLTLAQQDLSTRARASLAEERGLLPGLIALMDHSLPLIRAKAVVTVMLLGRLSPRWLLEACKAKLIPAVERLVRDKDEYLARALAALRAEAACLVPAIAASISEELAGRGGTASGTRRNTMGALVTVAGAGARPQPSKSPLALFPVMLHLLTSPHFRSAVVSGQLVAELASWLTLTAGPAAGGSIGGGAGGSVAASAGSSGAALLADFKTTLMPVLEALCQQGELVLQHYSAVLNTLLPALCGVVSTEAESGDTRFFCLRMVSDVLQTLLLDEELYGSAQQRAEGGGGVAAAQIDRVLRSHILPLVPRLLRDEDPMPLYALKLLGGLLEINLAYVADVEAMGLAAQFFEFLSLEHSNNNVHNIRLCRQIIAAGTMPVSQLLELQVAEKVAAVLEYASNNAVEPFLEPVLELVNTILQRDALEVSQGRSDGSLAAVFLEQAGVLLDNCAHPDVPVCSSAASATSNLVALFPQQCAPWLLAPENASALATMLAGQHLQPQGTGQPQGAGPMPVPQSLQQPLLEALATALEVPGAANASSEVAQLYDVVRQSAASSPDSTVRSIAGEVVGALSALLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7Z1</accession>
    <name>A0ABQ5S7Z1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">carotenoid 9,10-dioxygenase</fullName>
            <ecNumber evidence="6">1.14.99.n4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009730</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>all-trans-zeaxanthin + 2 O2 = 4,9-dimethyldodeca-2,4,6,8,10-pentaenedial + 2 (3R)-hydroxy-beta-ionone</text>
            <dbReference type="Rhea" id="RHEA:26393"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:27547"/>
            <dbReference type="ChEBI" id="CHEBI:53171"/>
            <dbReference type="ChEBI" id="CHEBI:53173"/>
            <dbReference type="EC" id="1.14.99.n4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.n4" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF89">
        <property type="entry name" value="CAROTENOID 9,10(9',10')-CLEAVAGE DIOXYGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI66025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="546" mass="61467" checksum="A0C93C1C2BD41DED" modified="2025-10-08" version="1">METHSFAQRSVQQWLLYALPATCLSASYLIYKHRRSLYAWAYQLIAKVPRPPGRAYISGNFAPVEDELLEELELVGGKLPEGLDGCYVRAGPNPFFKPVAGYHWFDGDGMVHLVRLRNGKATYCNRFIETERLKQEREINRPLFVRFGDMCGLRGLAIILLVKLGQAIGALKYLKGIGTANTSLAYHAGRLLALHEGDLPYGVGISCNGLVETMGRLLMPESWRNNFTAHPKLDPENGELLFIGYKIDAKPYLSAGIMDVYGNLVRQWNIHIPRPVMMHDMGATERYLVLLDTPLCFDPEAMIKENTVPFKFRKELPSRIGLLRRDQPSCPEGFADVEWFKIPGPGFHVFHISTSWEEPNGDVKIYLCQMDEIDLNMDGMKNSEPPYLTEYTLSPSTGVARRRRMSMVPVEFPVINPSKATRKCKWSWMAVMDESAEVPTMTGLAKMDLEAATGTDACVAMIKYPPGVFGGEASFVPRTGATVEDDGYLVAYVYDSKADVSYMNIYNALTMSPTPVASLRMPRRVPYGFHGTWVTEEQIKKQVMWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8E6</accession>
    <name>A0ABQ5S8E6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007454">
        <property type="entry name" value="UPF0250_YbeD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027471">
        <property type="entry name" value="YbeD-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04359">
        <property type="entry name" value="DUF493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117991">
        <property type="entry name" value="YbeD/HP0495-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="302" mass="32707" checksum="C8EDE049684A5EA3" modified="2025-10-08" version="1">MLSREGLSFPPPLMFNQEFVRGWRPCHPRAGVRLMRQASRHVHLAVRRASNQPGEPDAGASKPGLDDLPPELRDLHINEDGFLIDTKTGKVINEMGATRFDVAVRALRGELDPPSWVENSERTPGVILSALYQFPRSYLFQVVGRPTPSTTFANATNTDTDTDAFTTTAASSISCAVDQQASPVDGSFTSYTSPVVGSGSEAAPASGLRKNEPQDQQSDIAASPDDSGYSSPPCGKAAFARDVREVISRVTQASVEDGQVEVKERLGGKYVSVAVDVVVRAPELVSMVYDELAKDPRIIMRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8T7</accession>
    <name>A0ABQ5S8T7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Superoxide dismutase</fullName>
            <ecNumber evidence="3 6">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_009788</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="3 6"/>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42769">
        <property type="entry name" value="SUPEROXIDE DISMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42769:SF3">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [FE] 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="7">
        <location>
            <begin position="35"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="8">
        <location>
            <begin position="125"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="233" mass="25916" checksum="437703E99CA9B749" modified="2025-10-08" version="1">MALSMKIQASSLIAGQRRVRPMTGRRAVVIKAALELKAPPYALDALEPHMSKQTLEFHWGKHHRAYVDNMNKQIAGTPLDGKSLEEVVLASWNGGSPTPVFNNAAQVWNHTFFWESMKPNGGGAPTGKLAEAIVRDFGSFEKFKEEFKSAGLTQFGSGWAWLNADKSGQLSLSKTPNAVTPVVEGKTPILTVDVWEHAYYIDVQNRRPDYLTTFIEKLINWDVVAERYAAAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S975</accession>
    <name>A0ABQ5S975_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_010324</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="106"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="602"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="444"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="741"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="883"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="755"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="887"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI66480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="957" mass="100790" checksum="0993482F12CAC0B9" modified="2025-10-08" version="1">MTPSCLLGPQPSQPAAASHSIKRGGLLSRLFNSLPNDRRVESNSATPKVLTGAPWSSQCPAAVPHQAAAFSSIVASTISPGSGQTGHAPCSKVPSLNYKTNIEEKYIFGRELGKGGNGVVRVVTHRVTGEEFACKSILKVLKDASDKKKQGHLDSIRREVQVLSKLRGSLNVVRLEDVYEDEEHVHLVMEMCAGGELWHRIGERHYSERTVASFMRAVLRTLAQCHAQNILHRDIKPGNFMLLSEEERAPVKAIDFGLAAPFDPQQLPRTDLGLEGTPWYMAPETLRGEWSPGGDIWAAGVMAFQLLSGRFPFDDKRSPFAPAITAIWRSVLNDPLDFNHSCWAGISAEAKDFCRLLLSREVGARPSAREALKHPWLQGDSSERSVGKQLSRSVVARIQRFASGSLLRRSVLKSIATELLSHPELVIALPTPTSTTSTTTAAAAGALSAAEGAEDAVSKDQRQQKSPPRGIPTESADSTNVAEAPAAAAAAACGAAADSDATASWGVEGVEGGDSFKAGLIPPGAAALRTLLTQLRLDGAESALDRVQLGEALARMGYCLAPSEVSRLMEVLDTSRRGVVGRSALVASLMDWRHLQRNHTELWLELAEKAFRQLDSDGSGVLEVGELLAALRARLPPEEVRAALEDAVREAEAGGRGAKGLRGGSVAAAQGMDFDAFVNLLRVGSHDSLDMYDDRMSVRSGGSRGAALDRYNTLLAAASHQRSAGLATSCSTIATTDWSRHGGGGFGDASTHSCASDRSDVASMHDNDRSLHGSGQLLQQSSCKHAGAVPPPPPPLPASQAIAFRFDTDHVGKYGSGSKPKDTTVAATSVAEVSSAGGIVWRFDEGPPKAPLPPPAPVWRFDVGGGIRNRSADQVPAPAVAAASTSAGGGSGGLDCSGGGSGGGGSGGYQTTRPQGGYFDRRMHGSNLYHNVALRALQGGVSGVGPHRGGCLDTVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9C8</accession>
    <name>A0ABQ5S9C8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exportin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_010181</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044189">
        <property type="entry name" value="XPO4/7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596">
        <property type="entry name" value="EXPORTIN 4,7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12596:SF1">
        <property type="entry name" value="EXPORTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="10">
        <location>
            <begin position="26"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="970"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1045"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1206"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="970"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1217"/>
            <end position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI66389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1437" mass="145285" checksum="89BD46A9F2B57CB3" modified="2025-10-08" version="1">MDLDAAQAAVERACEEFQAPATAAAASAVLLQFRSSPGVLQACRHILDRSHSLDARFHAAAALREAVVREWAALGPDGRSALRSYLMSYLVSQAEDPAMQVVRSSLISALAVLLKRSWLELEEADARMHHHRATFFSDLEAATSSAAARGSSAGAAARRVGVQVLEAVVGEFCASSSSPLGLPLEYHAKCAMDLQDHFLQDIFRHAVALGRAAAAVHDGATCAACLSLMTAVLAWDFRRSPSASFSFATPQTLAARAAAAAAATATSGAMVTSAVVAAAAAAGSSGPSAGADWASLLLSPDTLDWVAALLRQLTAAAAAPGAPAAARQLVAQARGVVVALSSLSSDVFPREGAEVLTSPGGGVRGGYLRAVLRCVVAWVASPRQVVAAAAAGDEMPLWEGCKAILALAAAHPPRLLEDAGNDVVAAAAAGWPGSGAGAQVQTPTAGGFLALLQALTVEVIRSSCCPHTEQWMVECRDMLVEAWALMVQRDCSSPLTLGLSVGALGPPSLPGLAEGAAAVFSALVEAAMTAAGQQAAAEGEEINFENEAAEAAAEDARMAEAAMLARAAPAATFSLLAAAMAAAQQQLLAATAAGLAGGEVWAVFLERLCWLVRMAAHCLADSGAGETPLMPLPLSIAVEGGGPAVASVEGLTAALLGLPALALQEGAAAVLSPRLMESCVWSLARWSDTYLFPEEVEGLPAALVAAYATRGSGQAEAVVDGLARLAATCLVAFPGEVELHGMVCTTLLPVLSRRRPLCASLLECSRSWGELCGAFAGRRPQLSNGLAPKLQRWLSQSLCQAACGFPSADSPASIPAAAAAGHYITQLLGPTAAEVRALAARPDLYDMTARADVIGTLCGMLEVLRGGACHGAAAAGPPARAAGWGLLSELLAPLLVLQRAFGDDPRVGALLLKLAAAVVEHHVGYLPTDQAHMLMSWVLELLRQYRMGRAARVSVAAAAAAAAARGRRKAHHLAHSQGHHHLHRLHHNSSGQSEQRQEGALGSASLLQGGEDAARELRALLQLLTHITQRDVALAAAASDGWATASSAGSTPRSVNMSGALPVLGAPGGGGSSRGNTPKGPGVGTTATVALAAAAGGAAVCPSAAGGGGGGLTVGDNESGVAQVVLTGLDVLLPLLTPELLAAATAAGAGAAGGGGTGGKLARLLFSLLAYMMEVHPHAVVALPGPHFTTLLSCLAAGARGFSLVHHHQQQQQQQHTPDATANGSASLADGKGGTAGGVGGTFSTSTPASASSQPLTVTATGFDGVVVQSALEGLAGLTKYHHQSLLNGGRGFGAHQAPAAAGGGPLVPYLVQLLMQIVLMGDVGSDVVELAGDALLPLLQSDPVAFSNLGAALVQSSTPRVANQVGAALAKLLAPTETLGTATAATGATAPGVAAAAAACLGLDPRTFDLSRLSRRAFRERLCGVVSELRGLLRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAB0</accession>
    <name>A0ABQ5SAB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Armadillo-like repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010164</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055241">
        <property type="entry name" value="Armadillo_rpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36793">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36793:SF1">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22915">
        <property type="entry name" value="ARMH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Armadillo-like repeats" evidence="3">
        <location>
            <begin position="237"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="451" mass="49137" checksum="50BA1B3891E96A10" modified="2025-10-08" version="1">MMATQRKVLREWRTDMFKHTGKALTRCFRTCPRLPTPALRLHAPMGPMLPPSTSDRSGVTLRSRATIVSSATGADQGTREEEDFTEAERGADEDAAQMVAAAQEADDEAAAKAAAAIPAETDAVAATGQRIVSEDVEEVVEQVVEPLTFQSVAARSQAFLTTTLPGKLFLGGLAAAFVGSLLLATYRAWQKANTIRAKRMRQIDRNRELVEGINKYLLTGNRAGLTPGVMRKLQRSSGFSPVEVFRKYLWYLLRERKFDQGAVEDVVTIKVGLGLTDADAGEALRERATRIYDKYGTLMLNTEGLTLAGAQRKATCTALFRKVLYLAETERLVGPAAIEPGGSGVGSVADIGKMTTGRRQQQVAAALLEPPSRRRRAGDEPGPAICFSQECEGAESFNLNAKFIFIKKTMLALCACSLRGTGIRRDMEEPMRHGMAGDESVVRKQVCAPHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAS9</accession>
    <name>A0ABQ5SAS9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="16123" checksum="3FB13AEA2C959154" modified="2025-10-08" version="1">MDNTAGTRVLPLPQARGHDDIPEDPLGEAVALDQDFGATRPTPTAATRRSNRQRQVRGGASSGVRGSGGAGPSSDPAKSRKRGGAPSTAAAQRSDPNAKRTRTDDTYMPKWNCNRSEFQRRKLANVCLRCAESHSTKDCPLMPPFVPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAT4</accession>
    <name>A0ABQ5SAT4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010867</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000045">
        <property type="protein sequence ID" value="GLI66884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF22">
        <property type="entry name" value="TRANSPOSON TY3-I GAG POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="44283" checksum="FAC8984040E23568" modified="2025-10-08" version="1" fragment="single">MSEQLAVYRRCRRQYPGTAPCVLLPAFCRRKAEASLRGMRRVHRFEPGTVLFNGVDREGMLAPWPGTSYSVDVWYEAPDEGAVGYGPALSALTLFAGRSLVDNRVPVVQIGLNGRTLKALVDTGASNDFISVAEVNALGLTPEPSDCPQVTLADGGKHPIVGRITLSLSIGPLRVVTRPYVLQGLAETAPYIMGSSTLRQYRARIDMETATLHMCKGTLSIKVPFLSFKHVTPKKGEAEAVPMVNFAVTAMAARIEPEVVGRKEAVRLLWKGAHGLLLRPRVGCCATTAQISVDPEVEALLSQYEDVFKDIPGLPPVRPVDHTIPLMPGAQPTARPMYRLSPLELDEVKRQVTDLLAKGMIRPSTSPYSAPILFVGKKDGTLRMCIDYRGLNASTVKNCYPLPHVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB16</accession>
    <name>A0ABQ5SB16_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Procollagen-proline 4-dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_011299</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003582">
        <property type="entry name" value="ShKT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF238">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01549">
        <property type="entry name" value="ShK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00254">
        <property type="entry name" value="ShKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51670">
        <property type="entry name" value="SHKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="ShKT" evidence="6">
        <location>
            <begin position="96"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14300" checksum="98D8B788BA9B370C" modified="2025-10-08" version="1">MYLTTVEEGGETVLPHADQKVSGEGWSECAKRGLAVRPVKGDALMFYSLKPDGSNDPASLHGSCPTLKGDKWSATKWIHVGPIGGKKKINLGTPECHDTNEQCMEWAFFGECEKNPGYMKENCASSCKMCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBE7</accession>
    <name>A0ABQ5SBE7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Membrane-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_010385</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045288">
        <property type="entry name" value="At1g75140-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35464">
        <property type="entry name" value="OS06G0115200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35464:SF1">
        <property type="entry name" value="OS06G0115200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047283201" description="Membrane-associated protein" evidence="4">
        <location>
            <begin position="26"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="883"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="466"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="764"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="206"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="978" mass="102737" checksum="22858E18FE9C4DF9" modified="2025-10-08" version="1">MDIEIDFKIAILSLFILCGLRAVLADAPGSSNGNICKEENDTCAWLDPSSDYNDNLVGQQLSSSKATTLPPTAVTLSSSASTMTTPGNDLIDNDRMVSVASSAATDIISVPTETAETSGNGPKRIASAMAEALAVDTVAVEVESAAPQAMAAAVKQMAGEPVAAAVAVDTPSALEVMASEPTAGDSNEAGQEATEEPITSSADLALEELRSLMKQAHMALSRLETEVIRHERRIARLAVAATAPKASLAGSSAAALEPAVEAATAAVFRSLGMDSSSTSIAARQALREVLEQVLRGPPMALGQMDVGFGAASDRSVNHTAAASAGVAQEDWFGRHFAVRAAAQLLSLPPSPQGVPEAEAAVLSEPSIAAPATITCAFTYELPHPNAAASGGGALQYIAAGDAAGVLYILSPVDGSVMAVLSSGTDSAVTACSAFYVRRYESTVVTGHAGGQTCSFAVVLKEGDEDDAGENARADTHSKQQQRHHNSRAMRTGKYVVQSLRLRHVVESMPGGSLPWAAVEETAMFAGGSPAKKMMESAAETGGEQAADVMGEATESQYTGPPAPIMHILPYRLGNKVSGRRHVAVLDRAGNLRLERDNGTLRFWTRTNRTQLAARLVGTHVVMLSAYHATLMNTLQPRPPRAYTCHHLNGSRLLAASFDSHRSFRGYGINGRGELLSMVTPHEGGLATCKVHRASQLPWLDAGWLSNPSNIHSLLLTPLRGYLLLTHGSQRVLFNVTGVVRTAIPIVTSASDEFIADVANTVAPRPDSTACARTTRPSGPTGDGLIGGVLPTPPVVAVGTGSQVVLLALPSTPTPSTALGVFAAANDDTSYVMQEEEAQHPPPTSGLSELVGAGGASSSSGAPLLVLLDIRMPQPPISAGASKIWSQPMFLVAMLLVGLYQFWRMSNRRNQRRSMPSASRVTRNRRMYGAGYGGSAYGDTAAAYMYGENAVPPQSRSELRRVRFDRSMSRTVTYEDEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBP0</accession>
    <name>A0ABQ5SBP0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874:SF9">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="665"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="778"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="837"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1125"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="461"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="671"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="724"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="876"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="893"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1126"/>
            <end position="1141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1215" mass="124854" checksum="D662DD89B749487D" modified="2025-10-08" version="1">MSSAFVRTPLGRLKSMDGSGSGTGWSHVDNDALAAIISLLEPRDVAAIAGTCRHARNVARENSIWGPLLARYFGFGIQVQAPDRVCRFDIFRSLCAACKAGTNAVRRAADTAAAITPASAATHCRPRAVLGAVALEGLYTDGGMDMGDPSFWAGNAFQPNGWSIYCSRASRNVHCIAQLQAVRPEDAVIAAHRSELISLAADVAWLLFLGRMPARSVRKLLATAASAASGPANPQAPRLTRSADPQSQAVALCEQLRHAMRSSLFHWSTQGLTQLLWQLTFRFKVLQQQQLVGEGPDAAAGKEAAAAGGGAAAGGGLNGALPAFLLEQVPELLDLDGLVGALAQLPNGEGTGRQQQQMAGGGPPAAPLVPPRLTAVWSDIMQGRCLSGRRALVGIGERNEELQMWSRAAGEYLSSPALECTAAVVDGITVSRTGEFTCPVACGALFLALRGPMAGLGGKKSAVSGPGEPPAASAAVISATSQGDGGADADGGADADADGRFDSTIRQKQRQQAQQRGEVWRTDNKKQESVRDVFVGMVQQPYVQALSDMTTLVAVEAAAAAGLLPPVAARGSCPGEGEWVEFQRPPLQTSLPESCSGAGPAAAAATFATSAPGDRGASNLMSGGAAAGGILWPVVWFRFFPHQGLGDEGDQDMALPPLFMDMPPWHLVSDSDSDSDSDSDDGMDEDMDMPKPQEPPPLAPLPLGGWQKGPDDKEHNAEEEEEGSSSITISSGSSISSDDEGYGGAGEAGALVPDFIANPSAEWEYELDFLYGIEGQPNSYYATAGHVDSDEEEDVTGSAAAGVGSPKNHGPGPSGSGAAAAGSRNLQSAFGVAPRAAAPAAGLHEPTAGKRELVSGPGLSRCPNRQKTQPPPADGSGAGTSNGGSAGAGGGTDPADAGAGPSSACDGKAGAGGGSGLASYEVSGELFEGVSLRTWLDHLTRPDVARAVLASVAEVAGVQAIRHTFQNVQTALQPNALSRNAATATASARTARLVDADGGAKDCSAQLSQTSLSASNPAAPAPAPAPTPADATTATATPSAETAVAQGVAEATASAEAANSANAPVGPAAAYGAVNGLPEVVFLPPDESAERAFLGLPEVQTLLPPPPVRQQHETQHQQCRYSFERQATQRQEQHGQQGQQGLPPSPVHPPSGHLCMPLQQRWCGNTVLLKLIAPEDRSAAFGEDPDTAPNIDLQFVALRGVAFAVRPTLRLTHGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBX2</accession>
    <name>A0ABQ5SBX2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Suppressor of forked domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_011624</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PRP39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF5">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="307"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="733"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="733"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="813" mass="88938" checksum="97C6722F6F89DBEC" modified="2025-10-08" version="1">MDVEMEQVEHAAPVPAKETSPAPETAPAAAPPNPDLETYNQRWQTVQADPQDFASWTSLLTIADRLDDIEKIRTAYDAFLAEYPLCYGYWKKYADAEVRHLDNDMAAHVYERGVAATPYSGDLWAHYAAFKKGLPDVAADDVRSIYERGLAYVGSDYNSQTFWDKYFGFEQERGSTLHIASLYSRVLGCPVRDLDRYYTSFKNYIHPLAAAQIVQPNEYDTIRLRLESEHAVEHAKTAEAAKAAAEVKAEVEAAKAPAKAKAKADADLVTPAVKAELTAEVPKVEGTGAVEGTTDAEMELAVDEKHPDAAPAANGDVDQPMSEVKQEDTEMVPGTAPEVDDVPAGPSDAPPEQPEDIPNPEPDSKAADDPAVADATITPHAGVAGAEPTDSPKAEPIVVTDEEIKQVWVRQQDELYEQTKQELLRRKPFEDAARRPYFHIKPLDGVQLFNWIRYLDYMEARGDHAATVTLYERCLVACANYPEFWQRYVRFLERTDPKAAQPALERAVMGFCKRRPEIHLFAAHFDERHGDVEGARARYKQLLNSVAPRLLEAVTAAANFERRQGNLEAACHYLSELMSEEKSKESSRIYPFLAIHYAHFMRRNTGNLGGARKVLDDALQQCPGVRSLWEAAVHFEELVGGPEAVARALDLYDRCTVSPPEGAAAATAAGRCLPERDREELSARSVDFADMYGTIEQVKVVSARHAQRFMLPTTVTAEARAAATAKRVAEASAAAPAAKSARPDTVAAAGSGPTPAVAVPPPPPMMPPAAAAGYYPPAPPAAAAAAYSSYYGYGQYQAAPQYPGYGAYPGYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCM0</accession>
    <name>A0ABQ5SCM0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_011160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007581">
        <property type="entry name" value="Endonuclease-V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511:SF1">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04493">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="230" mass="24494" checksum="C964783B837C8E43" modified="2025-10-08" version="1">MAKQVITTDGVPWHLNADRPTERLTRVGGLDISFLEPQSCPRGQQHSPFARNAVPATAVNMAATEMALTAATTTTTTNTGTSPSSSSLVVIRESEPGASAGAVSSSGAQPTQWWVGQRDPAQDDEDSERSSVSCGPGVAALVVLSFPDMRVLYEDFEPVDLSIPYLPGYLGFRECDAYGRLLKRVQASANQPQVLVIYMILGIGSLVLAFRDCADAFASWAIKGCCCFDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCP3</accession>
    <name>A0ABQ5SCP3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="278" mass="29546" checksum="5210A7288212F31F" modified="2025-10-08" version="1" fragment="single">VKCGHMAQCEHRTNGNGVLPCSLSFSLVPGKVNHTHNSGIVVETCIGQDYLPTAQKRVWWLNLRSCFRPSHPRHTCADICANNGAPIPKLRTIFLLGSAAQLFVGNKADTELHTWLSTDPAAFHAFLAASRFAAEGKAFRLRLQITLHHTKTSTLLPQRLLLADYGTQQGELVIQSCRVQPDDVAGCQNARPAVLIRTASRASGSSASGCEPSAERSRLLSAEARSSSFTCRPEDDTPEGSHVGEHRGDSAADSAADSAAAAAVGSGLDCEHSTAIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE76</accession>
    <name>A0ABQ5SE76_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_012566</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="266"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="1180"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="15"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="236"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="488"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="615"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="681"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="845"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="615"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="686"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="701"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI68220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1387" mass="146643" checksum="0BF349278B4AD3F2" modified="2025-10-08" version="1">MKDFLACLLGCSKWSLNAGDPDSEETPTDGDGNSKSGHYSAFRCLGSRPSRGGAPAPATESAAGTKEQLPAAPAPPPFPSIQEVLLPPSFDQLLLNHSSTALLDSLIVAVTPACISIINLSGKMLHQNPQSRAYYGDRVGGSSSGSRAIISPHAEGIFANEAAAAGGTSAAAAGCGCCISNCQSIEAEADVLEQLFFLDPEKKESMRLMLRTTSSGGQAGVWKAVVRVPHSLTTGKSVLTPAPRPLPTATSPTAADGTAQQPSAQPQITLVMAEDPRTAASLRCREAAPQPPVQASWVATAPRRSSYPLAGIQSAAGLTMSIKYSQGSDRRLSQTNEKGTGFSCNGSSSAKTEGGVVDSRLCGAGAAAMDSDSGVNGCVNISGHLPPPSGSVQQTSSQGRLLANVCGRGSSTAGMGPLPKRMAAPLPRHHTSTPQTQIAHHLNRMLEPTLSASFDAGPKSSGCLTVDPLATSTFSTLVAVPVVLTPPQRTSFSQRPSSSHLPSCSRTAPAQTTSTTIRAAPNRISIDDPLYPTNRAHVRRRALTTAASCVETGSVGIGSLWDLPRTTISFRIRETLCTQLHKTSKGVRVPADDEGDGWDVTAAPQQERLLQLPSEQLQQPQSQTQQLKQPPQQRQLRSHTAVCTSRAFAFALSLGTSPLGPQSASSGGGAFSHTSVPLLTQKKQDQQQQQEQDQQQQQEQEQERGDVHLAQHQEQHPAQQREKRQQQMGVWLARHEPYIRGFTTPHSAAAGASRTTVTASSERPCQKMSFAGINGAAVGDSSERVKSADVSISPTLPALPAAISATRTPATTGTLITRDTADESNGTGVDGEFMYTYNSQQDNPLLLTTQEPDPGGSGAASHQQSWSPAPIVKKCNASSTILQQLLDSEIQRPATDVGSSSLQMTQKRFLNQKEDDPPTLFRLINDAAATAAAATATAAAAAATATAAAATAAATAATTATTHADTEAEADDAKPPVSLRHQVAGVAAADSAGNCAHRWESVQNPHPSPTSPSGMYGFCCSLLEPPGDPQPTDLQSPQHPLGTAQRWKGECWHEVWVMPAKSPLTGESIIIITQIDVTAKVIAERHLAMVMETEHRLVEQLFPRHILQCITEDWTAAGTAAEMEAAAAVEGQTPLAPAGDAAVRRQSNFGPQEQQLWGSERWQPAIRNCTALATSHSEVTLLFADIKGFTPMCKEVEPRQTMTLLNELYSRYDALLDKYGVYKVETIGDCYFVAGGLMREDEDGMTAVCDRSSKGDPLHAERVLAFAKAMLMAARQVLMPTSGQPVEVRVGLHTGPVVSGVVGTRMPRFCLFGDTVNTASRMESTGVPGAIHASEATFRRLPRTEQAEWKPTGGIQVKGKGLMQTYLWMASAAEGSLLESAVSQAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEX6</accession>
    <name>A0ABQ5SEX6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Palmitoyl-protein thioesterase 1</fullName>
            <ecNumber evidence="1">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Palmitoyl-protein hydrolase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012781</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002472">
        <property type="entry name" value="Palm_thioest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF8">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02089">
        <property type="entry name" value="Palm_thioest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00414">
        <property type="entry name" value="PPTHIESTRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046691983" description="Palmitoyl-protein thioesterase 1" evidence="8">
        <location>
            <begin position="20"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="334" mass="37334" checksum="C397B9227E01BE4D" modified="2025-10-08" version="1">MSPKLVLTSFLLWHAYAAAIRAPGPGQLELGEAFSPDLWKPLSNSFGSLPVVLWHGMGDSCCAPHSIGAIAKLIEFQLGVFVYSVNTGSSEYTDVLSSFWGDVNDQVAIVCAKLRNLTQLSGGYNAVGFSQGGQFLRAVAERCQHTGGPRMRTLVTLGSQHQGVMNVPGCRTLPLNSSHGACHLMQQLLGMGAYLPYVRDHVVQAQYFKDPLRLPEYLTANTFLPDINNERGPEGRNPLYPTNLASLERLVLYRFVDDETVVPRDSAWFSFFDGERLMPLREQAMYNEDWIGLRTLDEQGRLIMDDIPGMHMQFSLQWFAENVIRKYLTNRPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF28</accession>
    <name>A0ABQ5SF28_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="342" mass="34650" checksum="B5BDD2BFEAD20AD8" modified="2025-10-08" version="1" fragment="single">MDTKMESGGGNPPPLQPPSALLRNALRGLQAPAPAGPASFHPPPGAPGSATATHSGINQRETGDRRPEQPRRPQPAPAAEPGPANTRRPPQQQQHNHPNYDNKQPQRFPQQNRQGNHRAPFPVTPALTSGTVAGPYDDRAKVPDNVTAVVAPALEPQACTSFTFVDFLRSVAAPQQQQQGAVGSAAAAAKPQPQPTTATRQYAGRGEGRQQQQQQQPHRYDRPLSATAAAATAAPASAAWHSASPAGAGGAASGGTTIMPDPGTAPAAGSETSAPLQRRGIISFAPRDLGRLTAASAAAGAASAAAAAAAAAAAPSGVSGTRAAGPHSLQGRWGAAFRGSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFV3</accession>
    <name>A0ABQ5SFV3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Integral membrane protein TerC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013121</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005496">
        <property type="entry name" value="Integral_membrane_TerC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022369">
        <property type="entry name" value="Integral_membrane_TerC_rswitch"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03718">
        <property type="entry name" value="R_switched_Alx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30238">
        <property type="entry name" value="MEMBRANE BOUND PREDICTED REDOX MODULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30238:SF0">
        <property type="entry name" value="THYLAKOID MEMBRANE PROTEIN TERC, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03741">
        <property type="entry name" value="TerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="38856" checksum="8CAC16391F93D7EE" modified="2025-10-08" version="1">MPCPLSQPQRWLHNCSAGLRRWSLAHIRSTSSRLSTAIVHCAQRPPQVDDPVQQQTYLVESRQTAQEKELQFISTSLPRTESRYEALAAWVGGAVAFGAGIWYVQGAEKAQEYFAGYLLEQSLSVDNLFVFVLVFNFLKTPVEAQNKVLTWGIATAAILRAVLIVLGVELVREFQPLLLVFAGILLFSSFKLLTTSDEDNDDDLSENLIFRFCARFIKVSETYDGDNFFTVRNGVRMATPLLLTLAVVELSDVVFAVDSIPAVFGVTLDPFIVYTSNVFAILSLRALYSFVATAMGELRFLDKAVAVVLGFIGSKMVLGFADIEIPTDVSLLVVGLVLGVGVGASLALPESKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG94</accession>
    <name>A0ABQ5SG94_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36706">
        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="134" mass="15352" checksum="202A4ED449911CE8" modified="2025-10-08" version="1">MDDFQKVYSRRLIRVCRGIAGVLLVGCVSVMTGLLDVHVYKNEVEYARETMAKDEFKPHRCNDRLADMLEVIEAEDGNIWRDLRPSFRNWWACMHSAEGGEPAMPYLMLTAKDKQYIDPASRGVREDGVPQLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGJ3</accession>
    <name>A0ABQ5SGJ3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="141" mass="17318" checksum="86ADD87C88A9C05E" modified="2025-10-08" version="1">MRSGMSKKMRWRLLLRRRPRRSSRTEVWSCSSNVQQQQQQQEDIQLEQQLIGREQQLQQQVDEKPQHVAQQQDGKLLGNMRQFTRQQDEKMQQFAQQQDARMQDFIKKQDEKMQHFMVQQGEQEWDEEMQHFTWQLLGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGK7</accession>
    <name>A0ABQ5SGK7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013604</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF147">
        <property type="entry name" value="CGMP-DEPENDENT SERINE_THREONIN PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="321"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="719" mass="74956" checksum="ADF6ACD05720404C" modified="2025-10-08" version="1">MSARRLSVDGGPIYPQNGPASPGKPKMHLALLKLSTANAFAKALSAASATKGAGPSSSSLSPEAPSGFHGVGGTQNFPEDNVAFSSWAAAIPAADSVPPLSPQPRSPYYGMYAEYEDIMELLHSGVVADGSARRLLSHVAPEQLVHVAHLGIGACCSVDLVAVHCSNGSRLLAAAKSCYLPPSDPRLRASQREADLLRRCADCPFIIQLLAVLQQEGLHDAGPAATPPPPSSPPGSCSPADTPARTSLQLSSSGGGLSELEPGVSVMWNSCVRSGEARGLDGAHSLLSMHSGAVMAATMSSPAYQAWAQWRGGGAVEDLEWQTMQRLAAGATAATSMADSSGGGALFEGLGGGTFCGANPRAYSGMGNVQMGSLAGVSQANSGGWCQAARSSSSPMGMVDDTLAGCGDGGVNYGNHGCGFAPHYGGGGPWISGGGSTGGNPPGRRCRRASMEIIDMYVGCTGMGGGSDAAAGPAGAVEPAVLYTLLIGWARCGDLRRLVQLLLGRSVAHQNNKVTGAAPAVSGLPLLMPEDAVRFYAGCLVLALEHLHSRLHTVHRDLKLANLLLLESGYVVVGDLGTAVDLNTVPGGRLNSRVGSPGHMAPECRDRQEAGYDTAADMWSVGACLFSLLTGQLPAGLGGPPNRQWTPQVSRYWSHELQDLMGRLLAWSPSQRPTVVQLQHDPWFRGFNWKALHGHKMQPPSNTPWRELLWWPKENRHLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH45</accession>
    <name>A0ABQ5SH45_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="432"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="532"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="217"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="401"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="465"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="534"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="629" mass="66225" checksum="3347742F3DAECEDA" modified="2025-10-08" version="1">MIERGIDNTSPFPSHSDHVILQQHPQAARLLEVMDASHETKQEEPITRSPASTVADDALTSKTDAAALAAAYRARIALAAATPASTAAPSNQLRATPAARLLTFVNSPLPTPTVHGSDQNIAQALDRKWRALYGSLKSNSQAAADVLMKQAQATSQLEELVQALFMQLKAGTEDRVRLQQLLLQERTQFMAQVEEAQQLQQRLHTSEHKSTAVQVVTETLQSQVAQARGALEASNRERQQLAEQLAIATECRTRGDALIAEQTAVIENLRRQLEEQASAMSSLAEDKAQLEAHLQDVLRAEQELTVHLRETHAAIEGQLAALREQLKTERQKRAAVVKRRSELQAQVEQLQGKVAAQQADMVALTDKLIRGGLLPRATSLATTQSTALSAPKPVAQVGAQARSSSTGAGAHTAQSRGGAAASVQCAAPVAAQQRARSTLRSNSLGQTNPKKASHSPIFSGGGSTIRPSGQEHTSSPSAASLEETAGPDNAPAVAFHVPSSLPSPEASFAITSGPGMAAGGTEQDLSYSREGHFATPAPATAPAHTSSVVRAAAEPLSPPITLASAREARGLSMEPGSAGLNRRRSASPEASRPSTNATQHARRAGSVAGRAPGGRATSPSRGPGNTWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHV4</accession>
    <name>A0ABQ5SHV4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013273</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040848">
        <property type="entry name" value="AAA_lid_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041190">
        <property type="entry name" value="Midasin_AAA_lid_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17865">
        <property type="entry name" value="AAA_lid_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17867">
        <property type="entry name" value="AAA_lid_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="357"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="807"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="1279"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="1599"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1929" mass="205869" checksum="D60119A151FE6824" modified="2025-10-08" version="1" fragment="single">MERVLRDFLTRFPDAKNEPGLERVLAQKASLDQVAAILEALLMQPIYTLALGGALRSCGSLLRLLSFLVEKRLALTTPAVDGGFVSMLVTVLELAPQCSSVVQRYLESTAPPQQLAGRSGTIGPGPADPDLVFTALRALQLLPGLRQHPSWAAGPFLKLLRHSSPQVRWAAVQAVALLLNMSDASRSTLSDQLLTASEQVAALESWQARRNAVALEQAAMWLHVPSYCEHCRDAVTHTGVQEANGVHGDGASGSDVGNIWAACDGFTALTVDPFGLPIARGFVEVCGIELPCREAEEAAGTATIEAISPGPGLPTAAAEAAAAAAKQPPPSTTGGHFFVATETVRRNLEAAALVLCQGLPLLLEGPPGAGKTRLVEELAARTGNGSSMVRVHLDDQMDAKSLLGAYVCTAVPGEFAWQPGPLTQAVSEGRWVLVEDINMAPGDVLAALAPLLESRFLHVPSRGMLVPAAPGFQIIATVTSTPTTSDGGAGMSHGAYGMSNMVKELLGGLWRTVRVDAPTDAEQMTMLGTAYPQLLPLLPAAVATLCFVQAAGGHDGGFVECSGGSPSHQAPGFQLTTAGPESVATLGDRREVAAGSEASQRRCGTWHAMAAVALEATGLKRGALALHLGRHFSLRDVFKWCRRMKHLHDAELRSRRYNQLLLTRRKASEASLLSPAVADHQTSDELTAIDPRVREAAFMEAADCFAALVPRLEARIKLLKGLAALWCVAEGVVEQWEQLAKPQLSTAEGPSGPQLQVGRVVLPIAEDHRQQQDIAAAAAQGAGAGGTFARTGHAMRVMERIAAALSCNEPVLLVGETGTGKTTMLSRMAALVGARLVSFNLSQQTDSSDLLGGFKPVEPRDALGPLLPTFTSLIRRTWTRGNNDGYLTRVARLAERRKWSQLLAAFRGALTKMHDAGLVTGAADGSEGDSHRATVKLAAAAADDAAEAAAEKGGKPAKKRRVGGSQQQISEALLHEWRVFSADMAAAERAAAVAEGGFAFAFIEGVLVKALRNGWWLLLDEINLAPAEVLERLAGLLETAGGDSSAPGVAAGSVTLLERGDTVAVPRHPNFRLVAAMNPATDAGKHELPAALRNRFTEMWVPEPGAREDLAALVAAYLAGIGAAPSVEAVVDFYLAARSEADSRLVDGAGQKPAYNLRTLARALDYTRTAAPSYGVQRALYDGAAMAFLTQLAPAAAPRMEALIAKHLLPGVRNVKALLRSPPEPLAGTHVLFEAFWLERGPLDLPAGGCEEDGSGRRFVMTASVRQHLINLARAVLIRKHPILLQGPTSAGKTSLVAYLAAQTGHTFLRINNHEHTDLQEYLGSYVSDAAGRLVFREGPLVLAVRRGWWVVLDELNLAPTEVLEALNRLLDDNRELLVPELGEVVRPHPHFMLFATQNPPGGAYAGRKVLSRAFRSRFLELHIDDIPDSELAEILEKRCAIAPSYAAKLVAVQHELQRRRNAASVFAGKHGFITPRDLFRWAERGAVGYEALAADGALLLGERLRMPSERAEVVGVLEKVMNVKVNLELVFSSEGDAPLRALEAALQEQQQEQDEKTSVAGVPDTVGATATLQTALKGVVWTRSMRRMYTLVERCLRHSEPVLLVGETGTGKTTVCQLLAVMRRQHLHILNCNQHTETSDFLGGFRPTRGRERAVGHLRAAAMRVATSPLLVALGIPAPLVPEELGPQAIAPLAAAVVQTAESASKALAELTVRGRKDAQKKLRAARKKAAAGGSDSGAVDLDEQLKVLQADATTATVAATDARAPFAWADGPLVTAMRRGDMILVDEINLAEDAVLERLNSVLEPGRTLTLAERGGEGAEVVVAAPGFRLLATMNPGGDFGKKELSPALSNRFTTIWVPAMDDPEEMRAILEARLADSFNRSAIGERLLRFWQEYLRLQPSGARQLLTVRDLLAWVSFVNATSPHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI83</accession>
    <name>A0ABQ5SI83_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013762</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039184">
        <property type="entry name" value="SARM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22998:SF1">
        <property type="entry name" value="NAD(+) HYDROLASE SARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22998">
        <property type="entry name" value="SARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="7">
        <location>
            <begin position="593"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="546"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="776"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="908"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1071"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1499"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1598"/>
            <end position="1997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2010"/>
            <end position="2133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2159"/>
            <end position="2181"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="732"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="485"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="908"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="926"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1506"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1605"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1635"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1705"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1742"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1780"/>
            <end position="1795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1802"/>
            <end position="1811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1932"/>
            <end position="1953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1957"/>
            <end position="1967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1968"/>
            <end position="1988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2010"/>
            <end position="2030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2042"/>
            <end position="2055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="2056"/>
            <end position="2070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2079"/>
            <end position="2091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="2092"/>
            <end position="2113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2181" mass="232270" checksum="BF19A5553F64D05B" modified="2025-10-08" version="1">MEGDSDEFGLGNTGDLEIDHHDTSGLSAQARSQAAESGLFSTDGDDDFGSGNNGPPSGGLSAMPPRPLPPGPGNQRGSSGLFSGTDDDDFGADDPDLPSRRNAPQPPRPPPLPSSNRGGSSGLFSSGDDFDADSGDLPPRPGPELRPRAPPPAPSRRSDSGGLFSDDGLNSGELGLSDLGTEIDHGGAQPSRRLPPPTGDFTNPSLSLDANDSDIADLLGPQSTNAAPGPALPAALPPRPGPVRTVTAPLPPPPPPPASGGYGLGEDDPELAELLGETPRGATTRPALSSGQPSNRRITFQEPIAAPLQSPALAPQQQYQRVQQPQAVGLESGSFDDDSGDGYGLERDAKSGLTELGSRRDTPSDDFASGDDYGSVTFGGRVPAVATSPVPSAGASFKRIPSPGSGYDPVQDPLDSPVARGGRPPPRGIPPRRQIGESSEGNFNLIDEYGDDEDGYGSGMYGNRGDGRSDRRSGGVAFRRIPSPTRGPPPKRPAAPSPPGDDDYEDEFEVEDDEDVFADDASWDGEIRFSRGGGAGRVNHVVNAARPRSARQPRSRGLQVAGDTRMRATSALPPRERQAALITNMMSRHPSTWDIREVASWVEFIGLGQYRPKFVHNCVDGGLLLSLGDAELSRDLRILPLGHRRALLAAIAQLREAAEAMEKNREGAPGAGAGPKSEAARLADLRQRAGEQEVDRRPSSITARLIPPEPFLGPAAGKVTVYEQRAKLLYQLDRARHRAAQHAAIIEQLSNNKALTEAQMAEVRGKLKDLETKHKDEFSPTHRRGMTASVSPGGPGGSRKGAAALAAAEYGPDAAVPWLPVGRGTRSVNPHPERFARDGEDPTVDLTFRPRVGRVEEVPAGAVRDIVAKALDSRTPFWQRMDFEFEKMRKEREAREEARRKRRERMGVVRRGGEGDDEEKVKSPHWNNYWVPNSNKNNSNHKNFKRGSENEELTQYRSFAKDKTRLSRWFKRFVDDDERTEGDVALERLVNRMIRALVKTKEKVDSEVGDTFDKRIRTYFEQHGYKFETHTSNTIMVRARGTRPPVPARPDTTLYDFEEVEQYYPDYLKKKTQGGQDDYGTGADDEENETRMVPKRTKWKRLASIYHMERQKKIRAAIAMVKMSQFVLRHGVRWPPERDRDRGKKDVWVPSSGAGALHQLDGRDRTFNTVGQENISLHAKLKKALSPPQPVTFQMKMKQQEENEAKAEMLFALMGWPHSSGDPDLGPSMEPLLQPDSGGGEGFGLNGDEGGQAGPRRRWQQRLDFFGALDALLDRALELRHQQEEWWVSWNLARERSYGEEDLPLEPPRAEELNWGSDDPLMEMQNRAMERQRQEWLLEWERTRRKRERQATKMRSQGYREDDIEYRLGPQQEASEVEYAIQNHLVHNVELLARLKDADLLRFKEELKGTKKMMAVYRALRSQMFLDDTYARERRKEEALRAMYDSFRPGGNRRHITPEEQEAIYARLKSDEAKRAARRQELLERKRAEEKASLTPWYMLPRSVSRGPSQPSSRATSPSGGPANGRRSTRSPPTAEGTLTTPRRRPGSAARTRGTSGGGGPLEGEGSSRSMYVFGSSTPKSMWDPVGWRRQARGIGASLLDSPGLQGSPSTASISQEQHRTPGAAPWSVGRSAAATPTRQRPSTPMRSSGAVDSQKTPIRSPAPPQRPRSGQAPPASPSRLSFGGAGASARRPTSAGPPSANRISGGGGAGASTGGASAVPSPRPGASAVHPSRSRTQPANTGRGRSAAGAPPASPRRSTARVAFTAPPSPTRSSGGGSLPARGRGAAGAGPTRRGLMDGDTTSSASASPSPERRIATAPRGGAGLGSVAIRVKPPQEPRGLADTDSSPSPQTRLRPLPQAVSHPPPATVMHASGAAIPLAGAGVGGPGQPLSRRSSGASGTAGLGLGQGVPRVSSTGAATAAAATPPKRQPSATSNITSPQHGLSQQSSASSIMGPGRGTAGGSSGGVAAQVSSSGSASGTPTGHASVPGKDTAGPRALATSFAVGGVARASATGSAAAPSSAPGSRRPSTPPQGAIQQESSSTSKTSSTSGRAAGTGIGGAVDRGSGSGQSLAVLKSPQSRSPAAASPPSASPPAASPPSRSPPSHSPPSQSPTAASPRVAPSPQPSHSRLAAVEAEITEGSAGVALEDDYSLDEYEVDAGEGDQLDYGDEGDAGAEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIB6</accession>
    <name>A0ABQ5SIB6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1159"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1272"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1331"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1394"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1461"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1534"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1570"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1701"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1756"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1173"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1203"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1272"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1403"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1538"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1578"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1789"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1808" mass="178781" checksum="B115F0EB98A2B9E0" modified="2025-10-08" version="1" fragment="single">GGREAGQRSGALRSELQLCEGNGAASSMQLAQGIPYGVVASFGALAAASATAAARMSAARSALISQRTGTSGALPLAKTTASGPTVSGPTPSAATGGHAALAAAATTASIAAAAAVMPPAASANGGASLSTAAASAASAAATAASSGWLAIEELLEEAAYSLSQELPDAQGPSPLEALMASTSGGSSTGGFGSPPAALGMDLPEQLPESLIAPLSLSLTGLGLSSTASGSTAGSGSLVAAVPGGDKLSEMLEDMLYSLSQDINLEATQEAIISAATAAAAGLAGAAAELPRAAFSVYRSAAEAAAAAAAARAAAALGPTSGGSGGTALVVPDESQVQQASFDEQYDAGEEYDAADLVDIEDIEVGSGNPAWDPYGTIRATEGSQVVPDYGAAAAAATAADSSGDALANAASAAVEAASDGAAAVLPPIVTASAASSLADAATATAMAVVEAATTAAAGTILITPPQPSSAPPLLSSALVAAAEAVYADSSTDNVAGRGAASSSNATVVPSQAAAVPLQPAVTQSPALNGDSAMLGTDTYDLTAEDIQQLDELTAALQNLLGGPEGLAPIVDLPSGLGTASSGIANAAAATAAAAAASSPALSPDDPSLSAAEATMSTLAEALRRELGAVVANATDAVDADPTAVAQSAGQAVMSALDSLSLEQQINLSAMPQDLRLSTLLGATVHTALDLVESALGGIRSADSTLVGSLTIAVVLAVLIRSLVSIAVAALNHSQAAPAEGSIGSGGGSGSGSESAVRLRPVGVTALEAAAALNNDPQALLLDIRNDSDVREQGLPDLRPFRRGTGAITAPLPYCDFRTTPTLANPSGSLLAGSWPRDKAGGNQSSSTALAPMAGTVVVSVDPLFCSKFKQLEGLNRDSRVFLMDSYGVEAPEAVLLLRSDPEIERLLGAQGAKYIEGGFAGPEGWKLTGLPVANAPGPASEGAFSEAAGGLGLGGLNLSGLLSGVARLRLRRPSLFTRTLAVGAVGSAGVAVASSLDWGAVSRGAVALAAAMLLTDRALPPGMRPSGRIRQYLQSQMSETQLGGGSSSTAELSDLTAPRNRTALLLRVLDLGEAVGDAVIRAGGTAISVAGNVARDAIAVRNNSADAAVVPATKAGADDINVGLELETPPSPATGAAAAATIVLPHGMDLPRWSEAAEMAVAQRPPPPPSSSQQAGASTTDTAVSPPGKQPRSVSPLRPNWRLGSSTPADSSSPSLSAATAAATAVAAGPDTIAAAAAASGSGLLPPRSPSPSLAGRLSDALHNAASAIARAASPSRAANGSSSATTRAGSPGGGTSRPDTLELMAALATASALSNVDSGLLPGWPASSFPKASAGQVSTTASKDEEQADDAELEVLPSLSASSRPSDEVEAEVGEAAGNLWSNWRRELEAVAPPPPTLMSVDDGRTLDDGIREAGSSNRGDNADRSLYDSGSASFAASRLDMTSSSWRWGISPNEGADDIAGVTSAHVGSSNNHSDTGDGINRQQSRKVPPLRTSSPERAAAAGAALRRMRLQMDGLDDDGLDSGAYGTGELYGSSDSGGSGSGSGALGKRSGSGSVAVARMAPGRGGLSRGLAVGGQQQELSSPSGAEAFSPSGVEGRQKQPLRTASPQLAAVAAAAMRQLRAELGLPPNDGRDADAVVPRDWRRELDAAAASAAALGSVSESDLELEPAERFSLQGGGNSNTGNKVFSATGDRAAAVATTARLSPSPRNWRAQLEGRGQPESSNNSAGADRGRHRSNGGVPADWRVQIEAASGSGNVNTHGHDSSDDNGFLAQALRTTSPPGRAKLTAEERAARDARLHDWRARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIC1</accession>
    <name>A0ABQ5SIC1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013711</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="599"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="534"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="675"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="729"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="444"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="431"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="569"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="597"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="756"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="851" mass="87674" checksum="F8DCE1182337625A" modified="2025-10-08" version="1">MAVPSFFKPSFSEFIEAALGVNDSREGSLDACPADQQQLLQQSSSGAHLSASSNVSGNPSNPSALSEHFSFEIRAALPSTVTGQGSRGPGLGLAPGLVRLAAGPGDVPNAGTGSGGMALGMVLLPPVAPAPPVASGRVDGALIGVMAGSGPELSALGAATTSATAALAAAGRGAAWLPPYSQTLQLQQQSDLSLQGQLQIHMWAQMQQLEALQQHTQLQQMQMQQMQKSIQIVRGRGTGTGDGGGGGGGGAADISSGSHSFSQVPLAPVLLPPHVFQPPLQQQSLMSLPSHRHQQQTGMSFASIAANLTGAMGPAVGLTGDGGGGGSGAGGVETPQAASPRSSDLHSAALLQLPNIESAPDQSLGLGPPSNPRPCKAKRVSYTYGYTDCIASPISMSPQGGLSPTTSVLVGQLSLGHDRPPTSGMGAAGTPPDRAASSAPSSLLNLKEQQLQLLQLQLQLQQQQPQPQKLQVLQQQQQQQQNMQQQLQQGAKSVMPSVVRAAGALAGNFTTGLLVGATGAELGGGLPPCRPASKPGLPVAAGEGASGTSHSPSQDFYALGLAPEGGGESNSDGDPYLQQTLQYHTGSSGHQFHHSQTTRDKNREAQRRYRERQRGNLASLQSRAEEQAQVIQGLNREKRMVEQHNKMLLERIWKLEASLFDTQAKLKQLELQIQQRDQQQQQDHTGSASGEGGVRDMVMDGRDAETMVSEGPGGERVSAVIQGGDMARARHSNTGSPVPGPGSMGCRPTGSGTIRGNEDVKGDDGGRRGSTGGDGVAALAPTPFQLNSWPAAFTGLAAECRFGPNYEDMEMFGAEPLPAAADSPASQGDGGTVARGGVAGVVMDDDVSMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIF3</accession>
    <name>A0ABQ5SIF3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lipoyl-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013527</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF5">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="3">
        <location>
            <begin position="42"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="256" mass="26729" checksum="77F56F333BA22D42" modified="2025-10-08" version="1">MASRYVICQALQLLGNSAPSFSASVLPLQAARCISSSAFCLRPDVIVPSMGESIKEGVIATVHKQARQPVREDEVIAQIETDKVTIDIKAPAAGVLQQMHIKPSDLVVAGQVVAVVGAAEPVAPVTPVASKADAAIPEQASTSGRRAAIHFPPRFTVAGLRISDLPEPEYAAAIAVTGVVGSHIAPHLEQGSVQPLTPVSAAPATTQYPLPAAKAAPPPVTKNKLQYVTRPDGRGGPPRRPLTERELDMINLGGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIY5</accession>
    <name>A0ABQ5SIY5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methionine aminopeptidase 2</fullName>
            <shortName evidence="8">MAP 2</shortName>
            <shortName evidence="8">MetAP 2</shortName>
            <ecNumber evidence="8">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_014134</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03175">
        <property type="entry name" value="MetAP_2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="11">
        <location>
            <begin position="207"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="23"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="517" mass="56326" checksum="3F9A3BF46D6BC094" modified="2025-10-08" version="1">MSPAEYKAEALESAVAILDLGEEKDESVDASGAAGLSKGQLKKLKAKAKKAEQQKGESGQVSEEACSGSQATALEPAGPSELTVDNEDDDGGSGDDEGLNGLGDASSAGGEGKKKKKKKKSKKKASGAAVDGAAPDNGAATNASAASARPGEKLVQTSPPSIPVRLLFPDGKYPEGEWQSYKDDNLWRETSAEKRELERLNWDMLNEVRRAAEVHRQVRKHMRSVIKPGIRLFDMCEQLEDMVRTLIEVNGLEQGIAFPTGCSLNYVAAHWTPNAGDKTVLQYDDVMKIDFGTHVGGRIIDSAFTVHFNPKFDPLIAAVKDATNTGLRTAGVDVRLCDVGEAVQEVMESYEVELDGRTYPVKCVRNLNGHSIGPYQIHAGKSVPIVRGGEATRMEEGEFFAIETFGSTGKGYVREDLECSHYMKNFDAGHVPLRLPRAKQLLATIDKNFGTLAFCRRYLDRLGEDKYLMALKNLCDAGVVDPYPPLCDAKGSYVAQFEHTFYLHPTRKEILSRGDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJA3</accession>
    <name>A0ABQ5SJA3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="13500" checksum="9F3A1CB3FB15A1E5" modified="2025-10-08" version="1" fragment="single">GGGVDAAGAEAAGGHRGGSGRDGGGSQRGEVAVYHDFLGVTCRAVLGGEEFAPLICMNLELMAARFNALGEHLPAQRAEVPGYVDDEVPYGVYDFFRQDNLSDVNQLAPFVCRRNLLHLSCKITRCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKE1</accession>
    <name>A0ABQ5SKE1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="264" mass="29818" checksum="B131ADC0C7A8FC72" modified="2025-10-08" version="1" fragment="single">FSLHDVFLTKESPFLRHLRMHTGLVARSEALLKPAIDGLEWLIRVGQASLEYQDGNKDANAPQQPAGAAGTKVVRCRCDDLEGLAPGKLKAAWHMLRRVVVDESDQVGNSWAPPVTRAIGRCMALALRGAQQVPRCGMARVQDPAWEAAWQWANPEGGADEEGKEPVLCAQEVVQNRKGLWPAGPEEEDEKEVSCEDEDDEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEENEAEEENEEEEEEGDAKGRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKW1</accession>
    <name>A0ABQ5SKW1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="2 7">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_015108</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="2 7"/>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF36">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="10">
        <location>
            <begin position="277"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="342"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="210"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="495"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="497"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI70261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="527" mass="58765" checksum="0274302EF321E82E" modified="2025-10-08" version="1">MGNSCTCLEKGSWDGHPGVTASYPESSEVYQDMTQPLPHYFISSGHNSYLTGDQLFATAGTATILKSLRAGCRVIELDVYNGPICKHGGTFTSPVSFQECIAAIGQAAFLSSHYPVIITMENHTDVANQGKMAEILQKVLGERLFVPDPEDRRREFLSPAELKDKIVCRTSIKHSGTYHPEFRKLIYIRNTKCSSLQDMIKREDVVSSSFEETAMPQSDELGPDDSEAKVEKQKKKLLTKRRSWKEQLEKSPEDLGDEPEDALLPGERGMVAGSLQELYTYTSRHMARVYPALWRVTSGNYNPMRAWVRGASLAALNWQVWDKALWCNEAKFMDNGRCGYVLKPQWMRSPGAQLPQGTPRKLVVRVHSAHKYQGRNCFVFKDDLFVKINIWGMPCDCSSQKTRTAHDTGRLLVDKEFIFPLRFPEMAVLCLALKDDDSGGIGGAVRSAVADSLGYFSLPLSVLAEGDFKLVLKDPVTGQTLDAQNNWIKVGLRWEDGAEGEEDTDPQQDNESAAGEATNPMYAGRDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKW8</accession>
    <name>A0ABQ5SKW8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fe-S oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014763</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024521">
        <property type="entry name" value="ArsS-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026351">
        <property type="entry name" value="rSAM_ArsS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04167">
        <property type="entry name" value="rSAM_SeCys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43728:SF1">
        <property type="entry name" value="FE-S OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43728">
        <property type="entry name" value="SLR0304 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12345">
        <property type="entry name" value="DUF3641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="146"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Arsenosugar biosynthesis radical SAM protein ArsS-like C-terminal" evidence="6">
        <location>
            <begin position="307"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="450" mass="49140" checksum="95D7BA7EDA32558A" modified="2025-10-08" version="1">MAEVQSLCRCTVSGWPWKAALLTRPQSARVPTRRLGRAPLPHGQLLMTAIYATGGVRTSAAGESITAEESRTSLVPETLRDMEQDMELQRHLETLAAAGQQALTREERLKRQRSLDALGAPSFYATCQSRGVTALKRSPARILQLNIGLYCNQACSHCHVESSPRRTAEQASKETVDRCLVLLRNAKNHGLAITTLDITGGAPELNENFRYLVEQAAPLGVEIIDRCNLTVLLEPGQEDLVDFLARHKVRVVASLPCYSESNVDKQRGHGVFDRSIAGLKLLNAAGYGVDGSGLVLDLVYNPGGAFLAPGQAKLEAAYKSELEFAYGIVFNRLLALNNMPIKRFADWLVRRGQLQSYMGLLLDNFNPEAGESLMCRDTVSVRWDGAVFDCDFNQQLDMAITQPGEGGSSRPLSIWDIESLEQLTGRPIRCDNHCYGCTAGSGSSCQGSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL83</accession>
    <name>A0ABQ5SL83_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="646"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="751"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="835"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="904"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1042"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1209"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1475"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="497"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="581"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="657"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="754"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="775"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="785"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="835"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="906"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1050"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1073"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1156"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1218"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1246"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1267"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1321"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1476"/>
            <end position="1489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1642" mass="167991" checksum="81B96BBB8B16B921" modified="2025-10-08" version="1">MSNQVGFQDAEPDSGRRAQTEDADRSLPAPQRRGLALAAIHVVRGRWSLATKVIVATSRAAHMVDQDQQAFRRGTQDALGQVLRGELHVALVVEQPRDLASINYCQQGDRSLYSEEALRRRAANRTDPGVVEAVRDWWRWLPKAQTSLLTVVATAAVTAATSPPPPPATPTADEDSPALAALSTSRPACQDGQWALAASEALASPATAEVDPDLDPSFGRAEDATVAAAGGVGLEAAVPEVAEPEGGDKAVDGACNPPEVLEREKDSDPSRRGPGSGGEESTQVLTSPSSSPGSSLQLGAPQLQVEPNCASALPAGGAAGVGGLVDAASLHVAALDEPIYSAMVVVITWVLLPRFGIRADPEYDPSPDWLDDNEGLPYMDFKHFFDAMFDLADVWSIGVEATQYVDLLVTLLQDVQKLEATQEAFTRLLNKFRSAKQQPQQPQQKQPMLLPATLQLPQPHTAELQQQVFDHSPQTRTPGGGDADRKPDPRLPPPALATPRLTAAATAAATAAAPTPPTPPPAMVTSVQHPAAHRIHSLANHAAAPAGTTVASTPIVVAFPVRSTTAASTATLQPPPQRAVASTSRSTSPAPSSDVAHGRRDATFKMPQYGRVDVSGIRSPLADYLRSGIPQPDQATLELWRRRRASSSRCTAGGNGRSRSSSAQGGRTPYPIALSADLLLGPCGPGAREGTDGTVLMYEEYMHDMAARYLAQQQKMLVATSAGAAAAAGQLLAAVAATTATAGGTLETDKANGAAAAGDSSRTAASPPDKPTARGAHHHHHHHQQGMQQQQPLRNAPPAVAGAVPASPDETANSTTTSRNTLASTAAAAAAAAAAPAPSSAAPPRGSGATSTSPRASPFPVSGSPLPALVPPPLPALAAFTLDPLLMTSPHPVCVVGHAVEPPLSGSGGGGDGSPGSADSGGGGRGGRNPMRRQSRTFSTYAGATSMTSEGVPMRMQDTAATAATASEQQTTSGVAESDAAEAYAQAEAQAQVVVAFATAAYRLADPNVHSLLLPARLPVPPKDAALMPYCHPHPHPNVQVVPPARVFSNGGGGGGGGSSPSSGRVRPPLAISPTPTVSSPPHTSAAFSVMLPYGTSEADSGASARTGAAGADGSDGASGEWATNAWAPNSAFNAGENAGLLPPPAPALSPPATPSPTGTHRSAAAAAASAPTSVSTIAAAAAVAAAAASSLGGAVRRRLMSTAMSHFPTARHHQAVPVTSRSVGHQQSRPLGGGEAAAMPGPGHQLHDSDPLEVHPTHQHQHQHQHQQQQQQQFIHHNQQIQHQKQITSHQQHQHLQQFQNQQPWQTSPGALHSAAAASGSGGGGGGSGGPIANRWALTPQSWSTALTQTPRSAVRPTGSGATTAVAVGPSQRVGGVRRFSAAPGLHSGATAVSTGTSIGLPFHNTTRASCPSPLGVTLMYGDGSSAAANNSRRNSRSSGCNDEPVAAATAAAAVAAMAAGCILPSLPGEGPLSTVATASGRPSSPTAVQYGARRRSHTQEYASYPGNHAAAGVTTGAWTAGMSPDAAPVVRSPVRIHSSDPIGFADAVTAARMNVAIMADELMQRQAEHRVQAKLMRDRTLHLRPSTAALARHSFPNAFGAFLAAREATSMAWGPGVSISAAGGVSNGGGGINKETEHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLK3</accession>
    <name>A0ABQ5SLK3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015236</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07473">
        <property type="entry name" value="Peptidases_S8_Subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034204">
        <property type="entry name" value="PfSUB1-like_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046929183" description="Peptidase S8/S53 domain-containing protein" evidence="6">
        <location>
            <begin position="32"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="7">
        <location>
            <begin position="183"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="651" mass="68675" checksum="EECF15B7FF7D2AF7" modified="2025-10-08" version="1">MKTTIRTTRHVFWAAATIAMVLLFGSFVSQAVITPCEEGSGTCTLAASSSSPDAPPEYEGFLIRPANNSAAAAGAAGEDSHLQMLSDLGYSTQDIPVVGIDGWILVDPSKVDPEARHGGLAALGTPESTEQILQDFKSRGYDAEPNYVMTAQVVPNDALYSSIAANMRQINAESAWDISKGSKSVKVCVIDNSIQFNHPDLAGNVITVYDATSKQSNKEAGAPAENDMHGHGTHCAGTIGAVGNNGIFVAGVSWRVSILGCKFMNNAGRGTTATAILCINWCRQQGAKITSNSWGSPSPATKALKAAIRASQDAGHLFIAAAGNNGINIDVNDYSPAGINLPAIIAVGAVDSSDKLAPFSNRGKKTVHLFAPGVKIISTYPTNSSKSLDGTSMATPHVSGAAALLWSYWPSATAAQIKEALLKGVDVVHGAALSQTGGRLNLRNSLQFLQNNFPLLDVLQDLHGASSCPMMPEGYTFRAQQDILGSDIYCAALGNKTLKDLSSLCSATAGCTALNLWSAPGGPWHYCLKSEFPTTTFADLSKGFMPNRCQGIYVRSCGSEPCGYAFRAQHDILGNNIYCEALGARTTSDLASLCSSTTGCTGFNTWSDPNGKWQFCLKNALHYRHFTNLSTGSMANRCQGIYILGLQNGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLR6</accession>
    <name>A0ABQ5SLR6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="314" mass="31142" checksum="3CCD3215B8419E40" modified="2025-10-08" version="1">MISILAAETEVPPTVAPTSPCMLQNPAAGDIVKQQNAVAKYQKEAKKKQRGDKALGEACVQYALSVFLRDFARLLITFGQPAGSIFSVLADFTGLGYGGLSPQGDAYLNAYCCNMVRSSGISFAVGDNGIVTAGPISIDLGALLGVPAGAAGMAAAPITAALGALTSGAFPGAVPGAGPDTVVLGAVPSGVAFPGATLLPGAVSPGGVPGTAILGTSTFPGAVPGLAGAGTCTILDWLAFALQGDQKLMKRFTDLLTAALKLEVRLFTDALNGAARAGTAASRILERAARTGTATAAAGGDLTLGAVLDAVTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLZ0</accession>
    <name>A0ABQ5SLZ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016128</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF21">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="2"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="54"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="162"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="283"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="337" mass="33094" checksum="3A12576A510BD82E" modified="2025-10-08" version="1" fragment="single">RLPYRAGSCDGVLCIAVLHHLSSHRRRVRLLRQLLRVLRPAGGRALVTVWATEQEDPSKTTAKWTRIPAPDSGGGGGGGGGGGGGGGNDDRGIINATNAAAVPSPPASGGGDPAEKEKGGNGRCDGGDTGSGASGGSSGGGGRKAGLDYFVPWHLPFHRTEAARAARVAQQAGTQGGSGSGEGGGDGAGNGGKAVAAAAAAAAGAPKIDAAKGAVVFQRYYHLFEKEELVGLVEEALAAGPAEAVGSGEVDLEDVDCGGSGVVGGAVDGILITGADGGVVCRSEGDADHGGSTGTDSTGAGGPGECGGSQGVLVPSGVVEDTFYDRSNWCIVFRRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMS0</accession>
    <name>A0ABQ5SMS0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lipoyl synthase, mitochondrial</fullName>
            <ecNumber evidence="9">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Lipoate synthase</fullName>
            <shortName evidence="9">LS</shortName>
            <shortName evidence="9">Lip-syn</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Lipoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_016335</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine + 4 H(+) = [[Fe-S] cluster scaffold protein] + N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 5'-deoxyadenosine + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="9"/>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031691">
        <property type="entry name" value="LIAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004019">
        <property type="entry name" value="PRK05481.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009544">
        <property type="entry name" value="PRK12928.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="LIPOYL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949:SF0">
        <property type="entry name" value="LIPOYL SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16881">
        <property type="entry name" value="LIAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01058">
        <property type="entry name" value="lipoyl_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="173"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI71215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="439" mass="47022" checksum="157315963102624F" modified="2025-10-08" version="1">MAFTGSILFRASGPWISHQVQQLAGSALLASSSSAATASAWLPWPSSCSSCASVTTAAGVAHAPAESAAAAAQPVASTSGHQQPPPFAVEALRKKLAQGPDFSDFLTGQEIAEKYSVEAPSWTAKRRKPDWMKRSKLPGGDKYTEIKAKLRELKLATVCEEARCPNLGECWGGGDGHTATATIMLMGDTCTRGCRFCAVKTSKAPAPLDPNEPENVSKAIAAWGLDYVVLTSVDRDDLPDGGAAHIASTIRLLKQKTHGSLLVEALVPDFQGNMDCVKTIVDSGLDVYAHNIETVERLQGVVRDRRAGWSQSLRTLQRAKELGAQITKSSIMLGCGESQQEVVEALKALRAHDVDVVTLGQYMRPTKKHMAVSEFVTPDAFKAYEQLAQEMGFLYAASGPMVRSSYRAGEFYISNVLKGRREQQRQEEGGAAAAVGIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SND5</accession>
    <name>A0ABQ5SND5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_016027</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="822"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="633"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="954"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1240"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1345"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="646"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1265"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1320"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1357"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="849"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1368" mass="142067" checksum="3CA9E20D4F92B17A" modified="2025-10-08" version="1">MITFCCFGEKQRSLTAESHRGKPEAIELAERGETQLHGSVHGNASHSISFFSKLPSKPLIPQVSPLCHQDALLQLLSVLTSLDGGWWDQVDRALGATAQHLSAAHACVFLISGDSSYASPLALVGPPAPPLCIGTPLPLVTGTGMGPAAAATCHSAAAAVTSPPSGDVAAQAAGAGASAVGVAAVAAAAAGSTSGAPSCAAAMLLAEQDNGRLLRYMDVEEPGKSRGGSARGRGRQGCLPPSAPQEWRYLSIGKEPVEQMAAAADAEVAETGASSRQHRSGSYRHFAAVVIPAGNQSLGTLSLGAEGPGRPASWTPEVLHCTAAMLSSTLKLPQVDLACRALSELSSASTIHQLVRTLLGAASELVVTVTRIETQARVAFLLPDLGAAAIFQSAATDALGPSVRRRLSEVLLVDMAAPALSGGGGGGGGSLLGVLGGLLLGGARSSRTTACSATPVPIRSSLGGTEAAMSRTPSIAGAGVHVDAHRDSFGRGAHQPISEEVSVCRGHSLPLKHTLLSEALAQGTGLCISDCNAYVQDSKIYPRDLAVSRGAAPAQSLALATSCHGGRPLLALYATYHSILPQALLQTVVQELGQLLRALTASVAAKVEGALSAEWGYLRHQLLESMWNRSLRRGSASGSTQNPGMAATQTTSGADAAADAAGGHIPHCDSSIGRATSDATAVTAVAAACGTNGGGARASLMQGAGGGTLCDIPTHPRSSAASLGVLHNLAPAGDGSGAVGTNCSTGSLGLERSKGGGAGGAIGRDGRTSRRASFSLEAANSPRGSSKLAPLMATLHDRLKAAQAEQMANCRAASRMQDLESIRILEQIGRGGYGVVYRGLYHGSEVAIKVIQEVEVAAPGASGNPKGGGADGGLRRMNSVALENKHLHDAIELVAAMGMGGHPNIVQVLTFFTDVRVVVGDEPMDTLDRRLSGLDGVGELLRLTRMPSILDSLKKQPQQDEEQLQPPPPPPLQQQQQQQRTEVNGARPGGGVIVLVQEFCDAGTLKNAINQRAFYRNVIPVSEDLKSRSGHLQLNLKAVYSTLLEVALALRHMHGLLMVHCDLKPQNVLLKSSPRDPRGFTAKLSDFGLAKMMEHDEEGQLVIDEGVASGTLTHMAPESLAGQKQLNASIDMYAFGILMWQMVCGTRLYQGLNAKQIIRGVVREKLRPMFPAWVPQEYRRLAERCWHPTPSERPTASVVVAELEAMVDNCSRSSTSAAATVATTAAAAAAAAWRPPRISSHGPASFLDPPGSPLLHPSHQHAHAHAQQMQHLQQRQQLHQLQQQHQQQHQVAAAAGAGLYTSLPGSKAGPMQSPRRHQHQQQQQQQLLQQKMDGVPHVVASLRGGSQTGMGPSAGPPGLGLGPGRLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP24</accession>
    <name>A0ABQ5SP24_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016419</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="125"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="167" mass="19109" checksum="5CC94639A4EBBBBF" modified="2025-10-08" version="1">MGNQPGKILGGRLTKKDIERLQRRFNRLANSSGKVSISAFESMVELGGNPFIPRIFKLFDESGDGTLSLEEFTRALEYFGQLDVEEEQYKFAFRIYDEDKDGLISSEELFNVLQTLLGNIYPDAQLEQVVHNTMSEFDRDGDNKLDMDEFKALLSRQDLANKFSMSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP49</accession>
    <name>A0ABQ5SP49_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_016282</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031303">
        <property type="entry name" value="C5_meth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022702">
        <property type="entry name" value="Cytosine_MeTrfase1_RFD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF52">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12047">
        <property type="entry name" value="DNMT1-RFD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00095">
        <property type="entry name" value="C5_MTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RFTS" evidence="9">
        <location>
            <begin position="284"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="RFTS" evidence="9">
        <location>
            <begin position="642"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="461"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="570"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="691"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="934"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1036"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1303"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1565"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="122"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1043"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1062"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1305"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1390"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1410"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1576"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="1755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2123" mass="225372" checksum="9E70BA42CB8F58A9" modified="2025-10-08" version="1">MRAAGDLGSRPSLSPKKVQKAVPAKTKNDEKGIKRRADGTPAVGTADPVAKITAASANGKSVLSEGSKLPTANGGAKGSAAATAEAVAPKAKKMKRASDTIAAGDSAPPTGNGASIPVSKVKAKDGSGKALSRGKEDAAVKEVPTEGKAGKADTAVASVKPTSKGGKNNPSARKGKKRAAEAPSANADIGDDDDGDGEAGGDNARNDCAPAATRRGSGVGQRKSAQLVKTYRESEVSKDGEDEEMEDAEEMLKVKKVKDAATEEEALKLTAPSSSLGTGSAGGVRSLSDFTLVDPAGRPEPLDRLSTVQGGLFINGLIELPGARSTNKSRAKGAVGSGTASEDRCRVKCIGPIREWRIKYSAVADPKLVLHTELAQYECGKASTLYRKLLEPLLAQVELSGAVYRALCTDAGGRMDSSFEEVCCRVARTKVSKQYGGARFALKLNGRFLLEQLTAMTAASNPQLRADPATKDKGKAPADNGTTATCTYDNGPFAQGLKQALETEDHMPISKDALVMLMGRDAGGIRINADGGGEAGGAGGGGGDGGEAMVDDDYELACRLMAEEYAEHMNGGSRSAAGGRRGRGAAAGGSKEPYIQVREEEIANDYPAPKEYKGEEDQDEWDELILEDPELLGCGFHGADERESTRTLDQFAIYNADGFLTTLELVPMVAGLDANVSIFASGVLAEDNGDWGPAAAETGGAGALNEQGQNQGGAEDTAAGSGSGANGGSGGQRYFLTQIREWFVECGPEGQVVVNIRTDVSWYRLVRPQKRYAPWYRVVHRIANIAVQILKWLDEQDRASRLSFPDLVSRLASLPPGHPAFLSSKIDIVERFVVVHGQVVLNLIGRHYKETIRRSGFTKTLRTLLAERRHRKLEVSGKRGFHKAHVAVVENPVKNRQVRQAKKSNPMPATATAQVRQIWSDYFNALDEIQRQQRLEQQEQQGKPASATGTETSAPGSGAACTVDVAGGAAVEKSSGAAVAVVANTPPPVLRLSKVQPKTWTLGDCGSKDKATGRTVYNAAVCGNLRLTPGSVIRLGKQLSGDDSSDDNPSDDDDDGDKVSDDAAVSAGGASGNAEAEGGDGSGSSGADAARQHWEILTRGPFGLVQCIFTEDGATDAPKLQVRRLVHGRDTMLADVAAPTELFLLDRTPMPVVPASRGGAGDATALADVNDAATPPSAPGDVRVLSLNGRTVAELLEAKMLSRSATHTTRLESFKADVERVNKDAERVALGQPPVFVYRTVYCPLQGKFRELRIRDLDLGSYVEEAPSPSIIEMLSSGAGFIKDGVTYRVGDFLYVRADAVDAEERAEDDSSAPSDGDVDSDSDDQYDSDEEDERSSDEEEEEVDADVRDAARRKSHLVAVTRSRSRGQRKEKQSAATTEKGSVKGGSKGAEKAGKGKGKEADKRKELAEEAGENGSDDDEEEMEEDQAKPKHRKGHKQQTANHKGSNKGLRAFAVVQLWGVEASGKEGPGGAGGSAKGKAPAAAAAAVAVPSTIQVRRFYRPEDISRDVAYRSDWWDLYAPTTAPTPPPSLTLSVNEVYGKCAVVIAGQSRPQDANVLDTFQVTGSYDPRQPAKTHPPPASLPAPPSPPPASTEAAAGSKEGGKKKSSQVPTKGRGATVASGSAAVAATAAATAAAGKDGNENDADLQLDTLDIFAGCGGLSEGMHQAGVARTRWAIEYDSEAAAAFKLNNPDAKVFCNNCNVLLRAAMLKAGLEADCFADPICIDAAGNLDEATIADLPTPGSVALMMGGPPCQGYSGMNRFNKGLWSQVQNSMVMAYLSYCDFYRPRYFLLENVRNFAVYRGGEVFRLVVRTLLDLGYQVRFGILNAGHYGAPQSRKRTFIWAALPGEILPDWPAPRHVFVSAQLGLKMDYSGGGGGGMAPPGGYFFASGPPLPGAPLRCVTVYDAIGDLPAITNEAKEDVLQYTGEPKSAFQRAIRGSAGSELRDHIVKQMNELNMERCRCIPKGVPGADWRVLQRVVAEDPSREFFKGEPLVPFCLPNTADRHNGWRGLYGRLDPDGHFPTATTEPNPMGKVGQVLHPDQDRIVSVRECARSQGFPDNFRFYGNVVCRHRQVGNAVPPPLARALGQQLRLALKARRIQESREAANRLQELRKRRVQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPB4</accession>
    <name>A0ABQ5SPB4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009836">
        <property type="entry name" value="GRDP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34365">
        <property type="entry name" value="ENOLASE (DUF1399)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34365:SF7">
        <property type="entry name" value="GLYCINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07173">
        <property type="entry name" value="GRDP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="642"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="720"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="894"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1095"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1170"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1268"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1325"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1532"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="645"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="807"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="923"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1101"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1219"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1363"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1387"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1424"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1532"/>
            <end position="1546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1547"/>
            <end position="1588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1588" mass="159151" checksum="C9A9EC7C5FF0BD92" modified="2025-10-08" version="1">MASDSDSAGLEALATRVKQLLWVVLDSVPSELVPWSLMLVRLLHAADNFPRGGLYDGHYAAQAALRYELFWLPLATRAQQRAQATQVLIPPLDVAMAWLLHRCTAPGSYPADCEALLGRVLDSPPSQAFRFAGAHAYSQCESPAERQAWASREVWDAAYATRGSGTTDTPYTTTASSPAPGSEVPSPRTDLGSNVELSPRSDGDGAVDNNGSAGGVEGARRSWSSRRRFMLRRSSRGGSSAGSNEAQAAAQGDNKKLPAPAGAAVTMAGGTYGKGGNRNGSHGGSVGGGSSRGGSSRLRAQFWPPAPPGSPWDLTRHCPLGVTAPIGRSGGEYGVRTGGGGGEGGGYGDGGSGIINVALLGGRGRLAGELVRRMGELCQLLHSLLRPAYLDPDVLAQAVQRYCRFLMLQGMHPSVPMIPTTDIALMWVAHMAVHSAYRRTCHRLFGRLVLPGPGALLLEDSGLEFAETRRLYEHHFGEMYDPPLTRAVPLSIPHPLLSTPLAPFLSAFEVVPTGASNLVLAATYFDADASSLPSPTTKQMHQMHNNLQNGTVEYGNGTASGGGGGQLRCGAHALYLAYLLRRGGNRSTRSLVGLVMGQSHRRRAAVKYVANKTAGFRNFLHLPDSDCHIYWRKLRLRHVDPDSIPTGTATPSAGGSQPDEFNPEVDTAELYGEGGGGDSDMEADGDELVSHRLAEDQERQHDVVRQALAQAAASAAAAAAGDTAASGEGFLTPEGRSRRGSSGGTATPPHSHVPPPPPQLQTRPSNTPGGGGGADEARERPKRIALIVPLPPPSVSSATHSTLTFAATSSGLSSERVTVSGEPSAPPNEPPLATFAPIEQQFSGGGSHSLTGIVAHDENVAAPAAVPLPPSPSTTAAPPVDLAASLRAVAAAAKEPLRSQGAAPRNQLSNREEHQYQEPPTPTATTSMAAAAATAAAAAAAAQSPEPPTPGSDITVASDSAMPSPPVRRPSIERKKYEDGTEGWAYNNPYYIDRLLELGPLSPLSPLGETPPPASAAILAARVCAAASAAAAVASAAAAGVTAAQEQKAAPGNVTAAVSATKPGEAVAHGGGGGGGGSRDHGFSGEKWAVRVAHPKEESGGGDGNGDGESGGGGDVTIPTIPAGPLPKPDGDGGRTGGGDADADACISHNTGWGFEGDVWAVKVTHPIDGGKGGGGSGRLRHQSSATLVGNDGDGDGDGGLPAAPGSMYHSCSGSREDADVEDAAADSGGDDEDAVKHRDNMNTGRNLSEEGEDVFCTPSAAFASATLTPFSAGRPEKDDDVGGGGGDGGRDDAAAASALISGVGAGGGGGGSRMISAALMGGGGGGGGGGVVRPSSSGSHPSRLARMTVASPQEEEAVAAHTPSASLSRLPAPAPALTAPGTPGTARGGGGGGGSGSPTKPTTGNTRTYGLPPRPGLSTPRSGGSGSGGGGASTPKRASGAYSPADASYMVGFGGVASLVTRGEESPAFSKSLRERYGVVGSSSYSNAAAGKGRTIAAESAGGSAGGSGSVSGIGMDRASSSAGFASPLVSSSGASFCSAMSRSSGGRGSGGAATGGGGGGGTAARGGGGAAGAGRGDRVSGGGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPP9</accession>
    <name>A0ABQ5SPP9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polyprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016547</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF22">
        <property type="entry name" value="TRANSPOSON TY3-I GAG POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08284">
        <property type="entry name" value="RVP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="21988" checksum="B5C9B2D51D0148F6" modified="2025-10-08" version="1" fragment="single">MQCSTPATGIQGTVNGRTAVILIDGGATGSFVHTQWLARREVKAAKTVSNMSVKLPNGQEQDCPYVLPNASITIRTFKCRSTLVATDLGDFNVVLGKDWLAQWNPHVCWRSNTVTLRTPLTKHVLQGDPPEAVNVVHKVNTQDISTLGVDMTHINSQYADVFAEPTDMPPTRAVDHQIELTTSVPMSRNSYRMSQDELAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPV7</accession>
    <name>A0ABQ5SPV7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lycopene beta-cyclase</fullName>
            <ecNumber evidence="3">5.5.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_016251</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carotenoid biosynthesis; beta-carotene biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carotenoid biosynthesis; beta-zeacarotene biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lycopene cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.5.1.19" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010108">
        <property type="entry name" value="Lycopene_cyclase_b/e"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01790">
        <property type="entry name" value="carotene-cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39757">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39757:SF5">
        <property type="entry name" value="OS02G0190600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05834">
        <property type="entry name" value="Lycopene_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="573" mass="62754" checksum="B54D18DE5DF43502" modified="2025-10-08" version="1">MLLQAGGCPATLRLGSRSQLFRVTPVANPHRCKRAMNAASEKAGFTSGPYPVPEGPVSHFYRETESWPTEETVHLQQHDLEQQPYVDLIVAGAGPAGVAAAGRVAAAGFSVCVIDPEPFAHWPNNYGVWLDEFQAMGLEECLHAIWPKAKVWLNSLPDGEKFLDRPFARVDRPKMKRVLLERCAAAGVTFLPGKVAGCSHGEGSSEVQLADGRKIRGSLVLDATGHSRRLVQYDKKFDPGFQGAYGIMAEVESHPFDVDTMLFMDWRDDHTHAPGLEGMRSDNEKLPTFLYAMPFSKNKIFLEETSLVSRPAVGFPELKERLDARLKWLGIKVINVEEEEYCLIPMGGVLPKHPQRVLALGGAAGMVHPSTGFMISRMLGAAPTVADAIIDQLSRPADKATNAGSALRPSSESEAVSMATAVWHATWPLERIQQRAFLTFGMDVLLSLDLFQMREFFRAFFSLSSFHWHGFLSTRLSFPQLIVFGLSLFLKASNNARSTLLVRGVPGVLSMLVDLASTLKGYYPDMTSLRERKDAVDAAARQAAMTAELQSAIAASAPKAVEVSAKTTLTVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RL78</accession>
    <name>A0ABQ5RL78_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3741 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="35155" checksum="4A625207FC5F8427" modified="2025-10-08" version="1">MALDTRKNKSFGAADRTIAALKFHAELIREKAGDASAASDHNEILGNMVDLHHRNMCQVGESLLRFQLLGTRSTESDLEIDDFEVDSSGENNPSDGRDSVYQIRSQNTWSLLDSADSQTSAPAKQGSTLPAKKAVQDVDPVSLFRHMRAGTMSRGAASQYIHGNLKGASTLLSPPQAIPDDVEEVKVDVSLRCGKSFLSRPDQQAGAAYDMLAQIRGTKWNSDILSTKSSKSLNGKAGDVSALTTSRPELRGEVMLSRSFGSAIGGLTPGGSKSFTSGSVKRLRILPSVSVDATPCLLASGDCERVSFRTSAAGPSSGGLLSKLKKIIRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMI2</accession>
    <name>A0ABQ5RMI2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">indole-3-glycerol-phosphate synthase</fullName>
            <ecNumber evidence="3">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_000538</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_B">
        <property type="entry name" value="IGPS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001377">
        <property type="entry name" value="PRK00278.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854:SF2">
        <property type="entry name" value="INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="9">
        <location>
            <begin position="74"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI58782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="342" mass="37155" checksum="A8FC4C727CAE1312" modified="2025-10-08" version="1">MMLTKGQFARSPAGVRHSGRLARDSLRVTCNAQATMGEVTIRRRPPHGLETQPCGPHAFRVGGLPDENKPQNILEEIVWWKAREVEVMRDKQPLAMLQALAKKVDPPRDFRAAVLAKAKESGRPGLIAEVKKASPSKGVIQPDFDPVRIAQAYERGGAACLSVLTDEKFFQGSFLNLTRIRAAGVKCPLLCKEFIVEAYQVFKARASGADAILLIAAVLPNADLAYFMKAATNLGMTCLIEVHTEGELARVLQLPGVEHHLLGINNRDLGTFKVDLNTTKRIMDSEWGQQVKERGIVMVGESGIFTPEDVAFVQQAGCGAILVGESIVKQGDPEAGVKALLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN22</accession>
    <name>A0ABQ5RN22_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="238" mass="24707" checksum="2C5A8362D6193FA5" modified="2025-10-08" version="1" fragment="single">MNPAAVGLGAVAMQKPLAYANDLADHDVIEDDYEVVFDPQALKLSSSCCDAVDPPKRQLGGSKSFKDTSILGSAPLAAKLAQSTSAPTQSLLAPRTSAPDCHVYANGNPVISITPVSRPSSASLREPRTSPLNGKVASTVLNSNSVHGSTAADNDVASARQNDTEAQIQGDMHGCDHNNSMQPKTALLRRLSSLNLRLRPPSAKQEVVTGAACAGAYRATKDADGQASRASAEPYAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN55</accession>
    <name>A0ABQ5RN55_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000622</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="65"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="245" mass="26400" checksum="3ACDC97C86E3373E" modified="2025-10-08" version="1" fragment="single">GLRDQGLKDLPPSLAEAASALKVIDASNNRLTVLPAFLVTFSGLQRLVLTGNQLTVALPSGAGSRLTALKILVLDDNLLGELPEEIGQLKRLERLSVSCNRLRALPPAIGGLESLQALVVSRNSLEALPDELGSCARLEELDAQSNDLAVIPAALGQLKRLKMLQLDNNRIFAVPPDVLYGCTMLQTLSLHGCPIKPDDLQDTPGFKEFDERRKSKYDKVIAGGALLGNRGLDEGVDREVSPPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNE7</accession>
    <name>A0ABQ5RNE7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000905</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="414"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="701"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="702"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="824" mass="83671" checksum="F262AEAC006D9BD0" modified="2025-10-08" version="1" fragment="single">MENTWRSTPAAPGARSNVDSTRAASRTGIRPPGTKISAETDLCEVLVSMPLDGTGKEQAGSTRTHTATATAVTAAAAAGPNAPAGGIRAASARLTSVESDDNRTLGLRIVTAGNAPLDEPPTAVDERSALLGAWQRSDETSMYGKTEIKEAYIRPGMTGSPPAVASTAMAMNRLSVSGLTGAGEVTVAATGGGAAAAAATQGRSMRSFSPFELSSPAVAGLAAAGMTSTGPMVAAAVAATTRAGSGCTGAARGGSGVHVNGCEARVERTPSTLLSAATAAGDLHTAVPPPSPGAAGVSAGSGVVGGGGGGGSASRLMLGGDGGGGGAAGSGALPYGRSSSLVDDTAQLREYVREEQLRHAAEARSRGVGLSRTALKRIFMLAVTSGLLGAAIYLSRTPWGSHQRGGLPVWRWFLIAGCLPAVWYVPWFAIFLLVLLLESLLPVALYGSGGASSGFGGGFSRSRSAMAAQVAQGGVGGGGNGGEERGSEMAGPALLIYHILGVRQHMVRLLRSLSCQLLVHYAVVDCLGHDQRSREDFLMKAWSCVTLFLTASTLKSLIAKALSKHFHSKGYLQRMQQAVKQELVLMALSRPRPGRSVGNVLSHVGLGYHLANILGYTGHSTGAGGGGGGDGTGAGGGGKEYDGSGGGVVRSPFAAAADGSGVADSVVPPLRERLSAPGISGRTGDERRSVQSVPNLAALAPQQQHQLQMQVQKQQQGKQENARRASDTSELVGVAMCGHEGGIMYGSGMAAAAAAAAAAAARETSGSSGRRKAVNSAGEGARWRSVWRSLVRHLKHTYAKTKIQWDLSRPGANKHRGTNVLRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPR5</accession>
    <name>A0ABQ5RPR5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sec-independent protein translocase protein TATC, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001456</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TatC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019820">
        <property type="entry name" value="Sec-indep_translocase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00945">
        <property type="entry name" value="tatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371:SF0">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01218">
        <property type="entry name" value="TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="36395" checksum="C483AC172B19DD82" modified="2025-10-08" version="1">MLLNSSCHISFPSRKCSGSLTIGGTRSRGYRLPRCRANPEELRPVPIVREEGQVNSLLRSSKEELEEEVEEDSMLKKFLYPDPEELPVQESLAMPLWDHLEELRERVLVAGLACAAAILTCFVFSKDLVVFLEAPVADAGVRFLQLSPGEFFFTTLKASGYAGLLLAAPTVLYEVIAYVVPGLTKSERTFLAPIVFGSSILFYLGLAFSYEILTPAALNFFVSYADGAVESLWSIDQYFEFVLVLMLSTGLSFQVPVLQVMLGQLGIVTSEQMFSIWRWVVVGSTIAAAVLTPSTDPFTQALLAIPLVGLYMGGASVVRIIEQSRGQTAKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRA5</accession>
    <name>A0ABQ5RRA5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase small domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF154">
        <property type="entry name" value="PROTEIN N-LYSINE METHYLTRANSFERASE METTL21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="44704" checksum="585ED63A9FD5365A" modified="2025-10-08" version="1">MEGRKAKRARAEPATTAQLADSEYTVSGFLRSWAWKREHRPDRDRFQRPYVHSLKIPKSGPTEDEGIIKLTIEQTSFRGAEGFASTVWDSSIVVAKLLERNPDLVVGRAVLDLSAGCGLAAIAAARLGARHTVATDLGPNLPLLRRNCERNGAAVDVMEHWWGSDTTPLLQGGGFSSSRTEAPTDLAMKATQAAASPNAATSLAEAGAAAAASEDASGGYRGREGEAAECGEGRLRREVQERRGEREGLDLVIACDVMYQEAAMGSLVATLAALCGGEYDGNGGSTAAASATTKGVSMKGNKLQGHDKNAAASGLVTAKGATADPDAGTEADVKTEAEAEAAPLSSSAGVMNVCKDPVPASAAPGPRPTAVLLAHGRNRFAEAVFWRHAAAAGFRVEAVRWDDLDPVYRCTDVDVYWLRLEGEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRY5</accession>
    <name>A0ABQ5RRY5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001745</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="556"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="570"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="582" mass="64256" checksum="BA23501F8CBB2368" modified="2025-10-08" version="1" fragment="single">PSLLSAFSSLLRPFNNSTRSEPRFGDKEVQRDRKLVSYDIVDKNGKPYITVEVDGELRVFSPEEVSAMILQKMKETAEAYLGQPVRHAVVTVPAYFNDAQRQATKDAGTIAGLEVLRILNEPTAAAISYGLDKKGGEQNILVFDLGGGTFDVSILTIDNGVFEVLATSGDTHLGGEDFDQRVMDYFIKLVNRKYKKDISGDARALQKLRREAERAKRALSTQHQVRVEIESLAEGVDLSEPLTRARFEELNLDLFRKTMEPVKKALEDANLKKTDIDEIVLVGGSTRIPKVQELLKEFFEGKEPSKGVNPDEAVAYGAAVQGGILGGDGGEHLGSVIVIDRTPLSLGIETAGGVMTKLISRNTVIPTKKSQIFSTYSDNQPTVSIQVFEGERAMTKDNHNLGQFDLTGIPPAPRGTPQIEVTFEVDANGILTVSAQDKGTGKKERITITTEKGRLSADDIERMVKEAEVFADQDTAVRARVDARNRLESYCYNMKSSVDEKDMLGDRISEADREKVLSAVKEVLDWLDEYSDAETEDYKEKLREVEDVCNPIVSRVYRSEGGGGGVPTEQDAEEDLVDHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS37</accession>
    <name>A0ABQ5RS37_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M43 pregnancy-associated plasma-A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M43B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47466">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47466:SF1">
        <property type="entry name" value="METALLOPROTEASE MEP1 (AFU_ORTHOLOGUE AFUA_1G07730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="968" mass="105656" checksum="C534D201EE20CAA5" modified="2025-10-08" version="1" fragment="single">QLHINNRDQLYLSAVDLTALPPFAALFNSFFWLQPGPPVIQIPLPVVNVTYVKNKLLQYIPVDLQEANTYELIDNYVTQISRTEPVAQILANVLPAVGLNSTAIQPQAIQILLQSWFESNKVDASSLAATVDSLAQLAQGVLNFQSHVLSITSNLRGVVEMVRRRLEAMPNSTLTVGGQVIDLGGFRSGLGGRRSRALMQQRDVQALIKKRVEEAVTAVLSSPAAAPYVDELRSLYDPDIIPDLVCYLYDALIRLAAESDELRDIMTRLDGHYDLEVVPSLPIYDPKAKANLSTLAKPVPYDKRTVVAVSGPAWRRLLQIEPLQAALLRTKLPPTFKPATSSAMPKVLVPLVFHIMSYRQADGYGPPNFHRSPEYVDRLMNVVNAMALPTKFQFFIHEIRYDPDANPYLVRDSRFSWMGCASKGNYLFKECGDVMLGELDHPRSINVFVAGDRSNASFAGYGWVPSSPSNITSGHVAVLWSVVDVSQWNSQAAWEYGAKVMWHEIMHHLGLYHTFGKSAAGVVACSASSDDGIQDTPIVAGPVYDQRAFSTSSRSYCLDVFSKDLGSSWDYVLTNWRTRLGIPPADASHGFDSCPYQDGNDELGNYITYTHDVCVAALGHATAGQMQYMHNFADQIQQKLYSWGQYFAALNPAAFKIPPRAANPAALNGPCMATRGGCRCKASWTFKGQQLSGCSNPDGDGVGLWCQVEQDGNCQSAINGYWDYCVPEASEVLCTSELFRSPPLRPARPPAPQVPCGPDWTTVSGSVCSSEKWAIPDMNGEYDTAYLGCANPDDDPEGTWCRLKPGFTTLTGRIWDYCGPQCANYTGISSPSYPPPSTNKTLTQPPGNNLPPSENFTTNCATVYQGLPGGCSCRAAWGLVLRPPFASTGSLVILGSERPMGRRYTNARYCTVLPELQPPLSSSAVVAAAAVGVCETTGCLNSANNGRIMVCGTPCTALTWEDGEDILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSA1</accession>
    <name>A0ABQ5RSA1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002461</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="63"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="288"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="372"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="44792" checksum="A0C9BA830A2E26CC" modified="2025-10-08" version="1">MGILEKIKEIEAEMARTQKNKATEYHLGQLKARLAKLRTELQAPSSKGGGGESGFEVQKYGDGRVALIGFPSVGKSSLLTLLTGTESEAAAYEFTTLTCIPGVIHYNDSKIQLLDLPGIIEGAAEGKGRGRQVIAVCKSADLLLMVLDATKPLYHKQILTRELEAVGIRLNRQPPNVYFKKRKTGGISINSTIPLTHMDDKLIQRVLQEYKLHNCELLFKEDCTVDDLIDVIEGNRRYIKCLYVYNKVDMCSLEEVDEIARWHNSIPISCSLKLNMDGLLERIWDMMALVRVYTKKVGAKPDFAEPVVLTSDRGGTTLEALCRQIHNSMVQQFKYALVWGTSSKHYPQRCGLGHQLEDEDVVQIVKKKVNTGEDGRGRFKTTSDKPLRIADREKKPALKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSQ2</accession>
    <name>A0ABQ5RSQ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDK5RAP3-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002638</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDK5RAP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008491">
        <property type="entry name" value="CDK5RAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14894">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14894:SF0">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05600">
        <property type="entry name" value="CDK5RAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="263"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="322"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="592" mass="62795" checksum="00791A9F001FC143" modified="2025-10-08" version="1">MSEPAIARLVGEGVERPDASLPLDINYAKLAEWLVTRQKIPKDWHKRLQVIQAKAVEALKDIPSEVLTGLIDGPDAPLDYLRAVEIRDKLSSTAERTMFGGLSGPAGTWDKIVKAYEKQYVFLGECAGTLVQNVDFEIPYLRKQAAKLNQQIVDCDRRHAECLRNAALCAAKYKEECKKLGIQGVSVRQELRRLADELPGMLQGAVESLHEPRLQSAAEYYAGFSRFAHAPRGQRAAAVGPDLALEPSKMLPILFEIRERRTQPPALGPGDVSAAATSEPTTGGGGEINWDLSPKDDGETAAGTTVDIDWDVGPSEGEAAAGDGGGGGGGGGGGGGINWDIDFQDVTGGTAAVDSAFADAEAAAVAPVTINWDIEVDAVGIDADADAGDNLVGTGIRGEDAGPSASAATAGELTDPDAVAAARLERDGDYRARLMDDLLELRAFLAQRKAELSGSGNELLATALPADVREVDIDAVGTMLAAVQQCLGQLTTPRFRQLLLIATSQRYLDRLETSLKRQAGTEAKLQRAAVDVVARKQEARSQLVGLAPRLGALVERTRRLKSLSERSISAALGGKRTINILGEINNVLAAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSX8</accession>
    <name>A0ABQ5RSX8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002798</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SEC8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="5">
        <location>
            <begin position="25"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="265"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="565"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="631"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="768"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1098"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="576"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="633"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="655"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="779"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1100"/>
            <end position="1109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1502" mass="153404" checksum="ECC05261A4EAAC95" modified="2025-10-08" version="1">MAGVREQMEGATRQQALVDWAAVDEEIKSVPAEYREVLFHPLKHVVEVFSSPDPQGLKQELRDSCERLGYQLDAVVEGYHAGFARSIQNYSQILHLFQESKEQVDSLKRSLADAAKQLGAQSRYLNSQWRKTVSLESSLRLLADISTVVEVPSRVDAVLTARDWPRAVSCLLEGATLLAHEELRRMGALRKLRTDMVSLAGWIQDQMMDELTARIFSGSRASTSAAMAGGVGATVGLPGAPGRLGLGNNRAGGGGRGAAVLALSDPNQGPRLRGGSAAGRQAPGWRRKGPEPAYKLVTETLKNSRSASLELRNALTMGSNSLPGPGPGGTATAGGAATVVAAPAAVAGAGVGGGDIAVDVDAAMRHVTTRELVACLAQVDGVVDAKQHVTQTAQSEMRMLIRRSIESFAVQAGVHAGSGEHGVGALVGGGGAIGATSGPSLSPAQRVLAQRLVSYVAGACVQALGRLQHVLQELANAPATTQSSALDVLVTQRPGGAGLVGGGGGGGRESLRDGLLLQRRHRGLGGGGLTARERAAYLKEEYQNMWDIMQEQLQHLLGDLLQAGGVRGRPKRRPGRGAPQQGAADGATGGGGGQEYGALGGVRSLLSDLNPVHFLEQLAALAERTADLAESALGGGGGGGGGPGAGGGVQGSRPQPQPPQPQPQPPLQQQQQQQQIAGPKRPAGSSQLTFSFDVTIPGLAGDQAQVGAGGGLRARENDASGYTSLLQRALGDRPGSVYVLPDVYLPVLRLIRGAEELLRLPPDLQGITPGNGSGRAANGPAISSATPSTSSSSSGPPLVSSWIQHYLDEVVLEQLLPQVWVELRGRCTAALEDAEAFRAQVGFSAAAASAATLGTAACGGAAAPAGSGGAAAAAAAPAVSAIAPRSVVPIARFIEVVLQELMAAAAALPPFTSAFLAIAERILDRMLGAFMHVVRLLSADCPSTRLVSRSDVCLAMAAEGDALLLREPVAFAPQPNALPGAASAGGGSGGSASAAASDLVSFLVSLRSQERPLLVPPQVRPLGTVGGGRGSRYGFAAEDNSAEVELQYFAMRERPVGHERLVFGQGGSDRAVHLAAMSESLEYVAEAVIRLTANVANEAGGMGGGPGPHRGGDLRRGGNRASQQPGCASVPDLLLPAVQSYKALSGHCCRLLRLEALLLVGSHLAPVAATSHVCEEVDSMELHPSLGALTRAALRSSEDLTPFLQPAKRAYVLGPIAVAAARGIMWLLPAIHDISSMGVERMIRALSLLQPPLTSLVDIESSSSGAVAAVAASGARSAGATGVVGAASAATGSATGTAAAAAAAAAAALATAVPTVSGPGPSAGVLTALLVEPETRGERGRAFDKARAYYGLLLVPPDEVVRAATERPGRFLYAEWMALLQARVPHRAATDVALGALQRCLDRAYGLTPGAKVAEVVGAVVGAVQAPAERLGDVLLEGLVAGKDALLEGLVAGRDTLVAGVRVPAQTLQRVFKRIGAGARGSAGARRGAMAGKGGDGGVEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTH8</accession>
    <name>A0ABQ5RTH8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="139" mass="14569" checksum="DB05D850481E5241" modified="2025-10-08" version="1">MLAGALSQSYRCTSVAYVYTSHLLWIEHIYGIQQRLDQCRTDCTGNLTGCRFAITATIDIDIVVAASAISASGGAGGGGSVSTQCGQGQGDLSGGSDELPRIVDLRLAVTGGFHPLHIAKLLAYLHLRGRPLQVTALPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUG6</accession>
    <name>A0ABQ5RUG6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237:SF80">
        <property type="entry name" value="MOLYBDENUM COFACTOR SULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SULFURASE MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="140"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="939" mass="102387" checksum="612C77079F0DB517" modified="2025-10-08" version="1" fragment="single">SLPLYNFPKQVSSTAHQYTMLTVSKAFLAQRQGNKGQLLRVTLVVLVISIIWVIPVHDWNISNRRIYLGERLYQGTMRVLRTTVSSWHLGLPTVAATTPRPASLVVQAPVATPTCTSRGDTCSVGAHIFAGQSLAASPSAPQRGPAPSAAAPEVSGSATTTDRRWWNQEKDLWIEVHTEEDFRREVDTGDRPVAVDFFATWCHGCEKAYPEICRVVRDPELRKKFKFVKACVDELKTLAKNEGVTALPRMHVYKPGYGMLVSIDVPYSKIRFLKTNLNVIANNPGLGFALDPNGLTWTAVDSFRLFYRFVAPTGPLPSRDAVAAKAAAKAREVEKLQAGHSGLFDHLLKAANIGSGSGGAAASTGPRVGGAAAVTTNGNGNGNGAVASNSGGSPPLSTLMGSAAPGMATCKAPPGVNEAAYEAARRQFLEAHSAEYGYGGIIDSLYPKEVGCRMAPNEHYMDYTGSSVYCQTQLENVFSELRQCMFGNPHSANPSSSFTGDRVEEVRDMVLKYFNASPADYQVVFTKSATDALKLVGETFPWSEGSMFRYLRENHNSVLGVREYALKAGGTFQAVNESFVDRWAMRGDSGGDHSPPNTRFPAPTYSLFAFPAEDNFAGVKYPLSWVRGVQSRSTDSHRWLVMVDAAAYVPTQPLDLSQVPVDFVDMSFYKMFGYPTGLGALIIKTSLVPLLHKVFWGGGTVALATSEDNFHVLKCRPSDRLEDGTVAFLDVIAVKHGINMIQKLGGIRRVQEHVACLTEWLYTRLAALRHSNGAPMLAIFGKHNMPNHRQVQGGIVNFELLKPDGSIFSYKTVEREAAGAGFHLRTGAECNPGACYNYLGVQESEVESLAGKKEGCEDDVEFIKVQRPVDRGEDSISSADILRSIANSNISLAHPAAVALKWVEAPLGSVRASLGWWSTFDDVYSLADWIERTYKDRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVD7</accession>
    <name>A0ABQ5RVD7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003538</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046183964" description="Peptidase M11 gametolysin domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="3">
        <location>
            <begin position="140"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="565" mass="61203" checksum="CE66C53781888BB2" modified="2025-10-08" version="1" fragment="single">MKTSLAPAALVSTFFAIFLFDLEACAGRQLITGSGEQIANGTLLSVTTSSLPEGRDSDEFFLRQNDGQMYRLSFCSGVVSAADLAPNVRVAVTYSSIQDGVMHSCQKPRQLDREPPAAAGAGRRYLFGDEITTPIRPTFLIYIVNMCGYGKPATVTPAMLKEVFFGGGQNPSLSEYYNTCSYGQVSLEPSRVVIIANLTIPCNGNLKGLNFTFPSGNRFDTKSCGSENPVKWQYYLDSVALKKRTAIPTNFNHKVILLPAGFTATKPGCFAGAASIGPWFPSSSNANSYGTGLIWWSTELVGDLNAMFHEVGHTLGMAHAAAVANCSDDTGQCDHTCPMGAITSRQGIRCLNAPHLWQLGWGQPFRQLTDTDLPIGTKQVLTIPPQLTTSKSFVMVDLNTMQGNMKLYVAVRINTQPYDLPYARSQNGQPFVVVHSYNGTAKVSYLRTILLADVPLGEGFIHSSSGLVINFVDWDSRKGASATFCQRAAAMEQKCGDGIDDDCDYLPDEQDPDCVGKMTFDTAPLPPHPWPPSLPPPRRPTPRSPPLPSPPAPSPPPPLSPRDHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW22</accession>
    <name>A0ABQ5RW22_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carboxypeptidase</fullName>
            <ecNumber evidence="7">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_003925</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044997218" description="Carboxypeptidase" evidence="7">
        <location>
            <begin position="22"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="554" mass="62711" checksum="66420E0DE7B5F1D4" modified="2025-10-08" version="1">MKWRFILPSLLLFAVTVRTAANAGSIRLVEKEVHLHLDARYADTSLVDPPRRIAGYFKLNRTHDARMFYFFFQSRSDPKTDPVVLWMTGGPGCSSEIAIFFENGPYFINNDTRTLTETKYGWDILHNMIFVDQPIGTGFSYSEDWRDRVYNEKVVGDDMLDFLYEFYQAHPELMSNDLFVTGESYAGHYVPAVSSAIYRANELGQGPITLPLRGLAIGNGMTAPSIQFSAYADYALQNGLISRALHDSIQFWMPLCHWSAEFCDTHEWRIACVAALYVCQLTVSERILAANPGINVYDITKPCYGPLCYDMSAADEFLNRPEVRTELGVGNRRWEECNMNVNGDFMGDWLRSYDKLLPAMLDDGIRVMIYAGDLDLICNWIGNERWVDALEWEQSESWPTVAPEEWHVAGAAAGTVRALGPLSFVRVYQAGHMVPMDQPQAALLMLTLFTRGKVLTTSLDELDERLRARLEIRSRDLTGAQAMNEQPQAGLLAKDVRGINAGQRQETYRRDKPIDVVKVEAGAHGEAWAKEKVFNRVLGRHSVREPVQKETTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWB3</accession>
    <name>A0ABQ5RWB3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="135" mass="14974" checksum="4D13D9B5D20775ED" modified="2025-10-08" version="1">MGGEPNEAEILMYGGCFSPLFVGGWHLGGDLKSDTAPGEDDYLPINVAVSCADGSVFKADTNSYKQVIGPVLTDEEIKAKHRYTYDKYSKVDPSPTINFTFVVLLVMRGLRILLAIPYFLRRKKKVPRVALYLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWC2</accession>
    <name>A0ABQ5RWC2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004399</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19093">
        <property type="entry name" value="AKR_AtPLR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050791">
        <property type="entry name" value="Aldo-Keto_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625">
        <property type="entry name" value="AFLATOXIN B1 ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625:SF5">
        <property type="entry name" value="PYRIDOXAL REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="78"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="397" mass="43462" checksum="4F9BA24D42AF0936" modified="2025-10-08" version="1">MKSPLTRCGQQRTRLDPLIGRKDLGAHRRFRQIHGTTRAFWQQLLPGRSKTSGTFGPNERPLYRPSELVPLGSLKVSPMGLGTWSWGNRFLWGYDESQDPELQQLFNLVVSSGINIFDTADSYGTGRLNGKSELLLGKFLQEYPGSEQVRDGVHIATKFAAYPWRVLPGNVVAACRGSLNRLGLEQLSVGQLHWSTANYQPLQELALQAGLADCYQQGLVREVGISNYGPEQLKKIHAALAKRGVPLASAQIQFSLLSWGTAQQDLKALCDDLGIVVIAYSPLALGLLSGKYSVEEGGRLPDGPRGTLFRQLLPEVQPLVRIVKEVAEKRGKTPSQVAINWCMCKGTVPIPGAKDLAQARENLGALGWRLGAGEVAELDKAAANIKKGMVQNIFQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX81</accession>
    <name>A0ABQ5RX81_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004065</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF10">
        <property type="entry name" value="GH13992P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="30"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="236" mass="26297" checksum="88D0C320DD930F37" modified="2025-10-08" version="1">MFKKLFGQKAPPSGVSQSSASAANKTISAIQNLTEHEESLEKRKSLLEKRMDQEQEKAKKFLSEKKKAQALQCLKKKKLLENEIANLDNMIMRVNEQRMMLEGQRTTTEVVSSMHTAALAAKENMRVMKIENVDKVVDEINETTDQMRQLNEVFAAPLGLGADLDEDELLAELEEIEATELDKELLEPAPVPVTKVPAAAQAASQKMPSVPQKQKQPAAKTQEELELEALQAEMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXE1</accession>
    <name>A0ABQ5RXE1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="166" mass="17289" checksum="C7A7116C1C854059" modified="2025-10-08" version="1" fragment="single">SRQTLAPLPPKPTSTATTAPTAATGSSGSQSSSSAARSQVPKSTELMQTVAANLGWGGSPMRLAEVPEAFWKTVSPEVHTQITEYLFEQDYDANKYISAQLQELHARRGTQLHRTSVHVGLEAPNGGRLVGGLLAEPRDAVSPPLSTSSALAAYPPPTASSAVATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXM9</accession>
    <name>A0ABQ5RXM9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004544</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047322152" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="37324" checksum="B56159B3FD5F335C" modified="2025-10-08" version="1">MYPCCRTPPLSLLHSVFIMVTLLPPASMLGAFASSSIITCRLASVPSVSKTVTFVASSLGISTLRWAHTYAHFSTSPTTVLASSGDDAREHDNSTAAGDPAAAGSNMYTESGRSCESADAAFRDVMLTRLAALESEVAQLRAVAERLDGAKGNDEQPQGWQRKDRSIEEQRASSAAVGGTEGAPDGECGNRGPAGNNNGSATEAVAEGLSKLHPKITRLVQSGYLESLHHLRQRLMVDEWEGGGRQRALQRGVAEFEAMCSDKGLPLSWKELSQLYKDPQNLDVIRDPRVRSAARATLHRVLDERHEKVREGATQLAKELADVVTHGPGNLLAGLWGLLPLPGFMKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXS7</accession>
    <name>A0ABQ5RXS7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator complex subunit 15 KIX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="796"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="836"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="963"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="574"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="843"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="982"/>
            <end position="1004"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1328" mass="135587" checksum="444F8B54F230767E" modified="2025-10-08" version="1">MYAPGGPPPPRAYVMSDDRAQMRQYGLSILRQAFPNAKFTSRLPAVVEKTEEEVYKKYGSDQAAYVQYFKNKIDNVKNTLANAPRQQLQQPQQMLAAEQHKHAGHGASAQQHSHHQHPQQSQFPQAGMQHAMPPPQQLQHPQMRQPQIETESVIAVAEQSAAEQADDFNDFLGTFFAPPQQPQQPQQQQPQMGQPLYAPQHQPQQPVGAYAQQTVATGAQQQQAGQVGRPQPSAVTTAAAAAVAAAQQQQQQQQQMHIAGKMAGMSASHAGQMAGMQAGYPQAMGATQMGVQLPAPRAGGSSAAFPQPQATGGVMAGHGVPGGAPGQAGMMGAAGGMTVDQTMAGIYGGGLPVQAGGPPQHGSGGMVGIPGQPVSGMMVQMAQQHQQHPGVAMTVGGGAAPGGPMGLMPPQQSAAPHPHAHAVSGAPTTSAQIPYGMPQGQQQDMSYAQLNQQQHLMAQHQAAVTAAAQQQQAQQQAQQQAQVVQQHAQQQQQQQQPQQSAQVTARVASGSLPTQAAVAAAAAARKAGPMPMGPAAAAAAAAAAQVARAGAVASTGMPGVPNTATQQQPPQPQQPQQSQPAVAAPPMTTAGNPAYGGAPAVAGLAPPPAVGMPGVHGAVAAPPPVVPTSAIQGGPMPISQQTAAGLAVAPAAPVVPPVAAPAAASTVSLHAGHASASAPTSASAPAMPGGSHVVVMQDVMPHMIEEYWRAVAALKTRIHVLRAQLDKYTRAAEQQGTQGRAHRSKRILLEAFKRCTVDPNTPEYSSMPISATAVKLMGAVVESLDTNLHTVKNQAAVAGNGNGSGSGNGRPAALTQPGPAGTPAAEIALGLVASPEAARPAAATPPIPQAPQPQPQPQPQPQAQPQPQPQPQPQPQPQPQPQPQPQPQAQAQPQPPHLTGASVVAMPAAKPDGVSVGVAGAAPSPPLTNPPAAASVSANRAAAAAVVAAAAHNAKIRGLKPLSHRALPGKTGGESTGPHGRMPTDSDNKAALQHGKDVKLERSGDSASTGVDQVHRLLGLLAQPLPHSTLRVHASRGAADVLNFDWDSLMPSLPATACTQITAAAAAAASSGTSSEPPRARTISVCRVSGMHYIDDSGSVRTPSIAAGSDGTRGGLAVKWQRQAADGADAADGVNATSGTAARNPTELKRRREMVQAACQEVEEELEETVALRLVDKSSNKSSSPLILLTADGGGGGGTDDALVLDCDAVVGAALGEAEHRGAGAAESAAGSCGPRWKRLRICLPRDFPDASPVPTFSSTDAAYTHPYGRAARLHFQTALQSGHVPVRLCLRSVAKTWRDAVARVCGAVRAADASTAHVAATPATAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYI3</accession>
    <name>A0ABQ5RYI3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aspartate aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_005102</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="49"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="426" mass="46475" checksum="77F1193871BB333D" modified="2025-10-08" version="1">MTPEDRMEVLTRQICAGRLNGSTWEHVQQAPPDPIIGVTDGWRADPDPRKLNLGVGAYRTEEGKPYVLKAVREAEQQILADPAMNKEYLPISGNAEFNRLARTLALGPSSPGIQQNRVATVQALSGTGALRVGAEFLSTHLPSSFPRIVYLPSPTWGNHKSIFAKAGFQVREYRYFCAATRGLDFAGMVADLKAAPPGSVLLLHACAHNPTGVDPSPEQWKQLLEVTSEGRLIPFFDMAYQGFASGDLDADAASVRLFVDAGLELVLGQSFAKNMGLYGERAGALSVVCTSKEVATRVESQLKLVIRPMYSNPPMHGAAIAARVMGDPKLNALWKEELRGMADRIKLMRQVLYDQLVARNVPGDWSFVLKQIGMFSFTGMTRPQCELLVSKWHVHLTLDGRISMAGLSATTAPYLAEAIADVIKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYL1</accession>
    <name>A0ABQ5RYL1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="185"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="132"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="231" mass="26326" checksum="3751AD794114693A" modified="2025-10-08" version="1">MKPVKELSAALQARLAPPDGQVDMKRGGSDYSKLRSIIRSQKDQIQGLENEVAHFQSRQHRIDDGHRLELARLKKEVEYHKRLRQAADQRSYELEDQLSRLRQHQHVAAQRESHDDEKDSLILQSQLLISKNSELTTKVMRLESQVTQLEELKAALENVLNQAYFERDEMASELERVLSRQLRPHNPSVELEVEAEEDEGDEDGLESGGDGALPADGGNALERHPVEELRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYM8</accession>
    <name>A0ABQ5RYM8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="927"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1548"/>
            <end position="1678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1730"/>
            <end position="1777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2475"/>
            <end position="2560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2576"/>
            <end position="2698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2800"/>
            <end position="2904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1565"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1585"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2526"/>
            <end position="2537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2539"/>
            <end position="2550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2579"/>
            <end position="2618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2853"/>
            <end position="2863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2865"/>
            <end position="2878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2938" mass="309125" checksum="9FEAA1CB97B15B87" modified="2025-10-08" version="1">MYVIRQAGLCTGDVEMAGRTEVPAKDMQSFLGETASLLEQLSECAGVMECQEAGPTVNRDSLEHSAISAEMNALVWTTRLRMVLSQTFGISEVRTELLDLVSLGVLYNDALAPLLLQQMVRSLERGSWAEPGTGSAGGSAGAYGCRRGGDEENDWGEPMREGPDDTGWDGEELNGRAGAGTVKRFQRSKAACQLLGPTIADLPLALHLYSSCPEKCGELALQVVATIALLSSPKELSSWFAAYSAGGLTQVMDWLLEATLGWLTGPLSTDAADYEDGVRDAASSGGIFLQRQRDSNDGAAERVMVSPVAAAEHDQFLPLVQCGAVLLTAMSWSCPAVARGALVVVLDRLGAADEDLEGELEGHDEPFSCAIDDVGFHRCGPVWHVLRAIMSDMARVLAVRDRLKVLMRTGYEVGGMGSPSALQGSWYDHGPGPMAGGIPNEEAVRLVSELCGEDVDSYGHLLCQSLHLVALLAEHALYATSMRPAAAIRLVRLMAPLMPHSEDLVEMMLLDAANIAFEAAAAAAAEDSALRAAVRPASLNHGEGVADITSPSRAACQPCVPSANRPSDNTGRSPLTGITPARSVCSLRSSGARGTSILSEMLSLTLLGTSSGTPYSASAPLLLHQVCRAPANGVSSMLLDDASSGALTKRMEDRDGGHGGALPSAVLLALEVLCGVIMHGRRLAASFEPTVNKLLVGLIRLASRDSPLVSQVAHRSHCRSTVSCAASNDAYDVFGTAGRDGMSLASTNISVQIFSRVQAALAAAARAGVAVTPVSDADGTDPCMLYVAAVAATLRSCVECDMDDKNIRSITECGLSVDGVSDGKAGGARTQTPLPPASPGLRCSLSNCVRWQHARALPGHLLNDTAPYAGGGGAIGISRGRLQLRSTSLGSGQVAPIETGDQNVAFILAAPPVESLISGLGQALENMDWGGKPHGTGVENRGKDAPHPGSQQAQRRDRGRRLDEHITVLAELLVLAAFGDDCVATCQQGSRCSERTLSCQQMQQLSQQKFTDAEAEAVLQLRMQVLAALIAILARAPEAIALVVLRAAAPLLPQPLQEQRQRQLGKGLPEVVPPETDAVRCISKDEFGMGAAAAIGDRRVEGITTVGAASDILFPPNMLRRQTAYDEVVADDNNLLAAQSAVHEAVSLALLYRTRDDLVHATQDQMQTMLMGTDALAGPVCRQFDGHGNTVVRDADGGAGEAHMTATCANGGQRAVSTALAALLACHGALQDKLQQLAAAMVTPQGPLVESTVQEHKRLAAQTVAPGTGVTTPALIDQSVGMSAGVHPNSSFLGGTAVASILPLGDSGAKLASTQTAGSRDATKSAGAGKGSGFFVVRRLQAPAAPDATAAVAVASNQRHGGTPAPSATADRAAAPAAASMNSLLWSNKTALGHDSACDTLQSKLQHPLPPPAAVKAAIARPILNTWPVSVQVCTELLQQLLGLPAMSSLPLSPASQVQRWRLIAYGANMARRSLSSAGGLLMNLQPPVGERNTRSPGVLAEELLALGNVAINIALEAGAAQEVTPEAGGGNWNLAPASADVDTAITKASEPPGRKASGEAAGWTGTRAAGQTAPPGTAARGSFNPWQQGHQHQTPCPSPPPQVQSMGRNVAEDDAEKGGANKICGGSTVPWKGTMERAGRGCGSASDSRDVSTKKPGPAASDRMAEEPYEGQAGAQLDVQTHVDIPCTERKTTERRPRHIKMLGKLQESLLQFSRYKDVMASAFKPDSCRRSRRSCGGMDSNASRGGDSWHEGAGSESRRGGRKPCGPTRPTVHSHLLQPIQPQTNPEMSVLQRSKACTNHEAEQYLRMCASVHTCPPACILAGGHPGSHHGDDFGSNGDNCIRDEMQPGDAASEARAEALQLLATVARAAIVCLPSTCEGSPDFLQCSSETASKLTSRCSILLPLSRSGGSAAAAVAHGGAAPEDRGAPVANAIPLLQALLQRLYPPSVSGKIAREMLLDVVPHMSQLTFITLWVCEEPLAVASLCLLDEARRLLLRAHYPKPDPKKHGQQELGGRRHGLTPLAPPAQCPARVGGGELPFGGQQQQRQCKNGAVVGKVVDALVTLAAALPFCSSEGEPRYRQLFAAIAYSCSQMLLCPAGPGGGTNFSGKTARLLARLACRGALAVELLDLASEVLLGLSRAGRCPRLPCNGGGGVGQSGGRRGKSQGDQSICPYIHRAAARFARNNVLLAHARRGDCGFEASTAHVVAPWLTGSSTLQIAHVLLELSSAAVPRVQDAAVVMVTTGTYAPKMAGEELPASVATALTCCLQACGLLLNPPRAAISAAPVSVVTQPANCIWSTPVMGRAHAEAEAEEEKRRRLLVLAVRRLQTVAKCAAACMYVIKRRLSAALAATTAGVPAAAAPAPSATGRSVENDPLGNGPLCVVLSLWRYHSKDVAAVTEQLSAFPSRSLQRHSCDLHRLASAWKSAWHRLVLDVQGAPPPQEPGLFSDLVQALYKAWVRVCERATTCKRGKQLLSPPSPPDPSSHGAEPPLQPVPLCGSGRRAGTADISEPRGACHTSEGVARDIALRREKLSPNQQRQQGRPRPASAVKGVGNDEEIPYEPLLVILGYSSSEESSEEVDHDSSEVDVEREDDSANTSDDDDADRWDGDEADDEDRRGKVDGGDNTDVGSFHDKFNVADTSQRRNLQQEKHQQQSPLPPLPPQQQQKQQTHGKRRSMLVSSLRSVPAVPSAGNARLGMTERPLAAMTTDTGLDLPPALPPRTTPSVDVNVYIAAGKRRRITPAALVTTAKANCNKAMTTISVDPIHHAGAVQKQSVSAGATADAVNPSTTMAAATIESRHKRRSKTSVSEATAPVDEDADGNAATRGTGVPSAPAMDTKRRRRKHDSARRATQEEAGKRSSARRRRKKTRQRSSRNPFIAACLKETENRSSDTDGGWSDLEDFLVCQPERDYSAFLSKKHYYAADEDSEKDWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYS6</accession>
    <name>A0ABQ5RYS6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glycerophosphodiester phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_005230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a sn-glycero-3-phosphodiester + H2O = an alcohol + sn-glycerol 3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12969"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:83408"/>
            <dbReference type="EC" id="3.1.4.46"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.46" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08556">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044236">
        <property type="entry name" value="GDPD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47449">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE GDPD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47449:SF2">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE GDPD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="6">
        <location>
            <begin position="122"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="310"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="456" mass="48874" checksum="4EA8F96B2C9BDC5B" modified="2025-10-08" version="1" fragment="single">KQLMRRNEIRDNGTTKKSNMKSNVRFPSPVGGAAGLQQLELIIGVPFASRTEAIRTQPSPRPRTQHGLFRAFLAVIAISVATTILVVWYMRHELPVGQKRAKESEALLAPPSQSFCRRTRPPMVCAHGGDTTHAPPNTVSAFQASLDGGADCVEVDAALSQDGVLVVMHVRELAQLLGRSGVQVGDLTARDLLSLQWPSGEAVMSVEEAVRLTSPFVSCVTLDVKTYNDNAGQPVDEVAIAEALVGLVAATHCTNCLIWAKSDRVVRRVLELSPGQRTGYIVMNETEESRQLGMHHPLRFSAVQATADGAHVRGSGVGDPDQLPLTHGSVPEYGGSSGSSGRPPPSPVAAPRLLAPPAGWRLQPAVVGVHFAMAEPQLLEQLHDAGKQMFVWTINQAEVLRAVLDVGVDALVTNYPLTALEAIESRLTKCSAVQAAAVQAAVKHRQRPPLTTGDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ34</accession>
    <name>A0ABQ5RZ34_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_005089</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="25"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="27"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="215" mass="23573" checksum="2979FAB75A636347" modified="2025-10-08" version="1" fragment="single">LTIAEAVATELQLHHGIAETLLGMLEDKFKSSACHMWALSNIMNPRPPCSVKALLASYTGRSQKQVTDWFTNWRARHWRPTILSMPLHQRIRLGIAASRRFGRTTWTRRLSCHGDAFGPVGGGDDGDGGRIGGDVSSVGSIGAGFRRFMYPLSPSTTTEDEEEDGDDPMEDLYGILYGNDPMVDEYDDDDDVGGYEYGCDGGAAPLMPGQPWLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZW2</accession>
    <name>A0ABQ5RZW2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005546</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="37800" checksum="D3788A61B35E4629" modified="2025-10-08" version="1" fragment="single">TTAPAGTAGGSGGGGGGTVLPPLQRAIRSSVQQAGVKLFLHCGMCLLAIIALLLLDAALVAWIYLDRKQRLSRSLVIVAATNTGLVLLVLLISIVRSFMADRAAALEVAAARQAARGGAAAGNAGGGAGGVLGFLLSNFVTPTLQTMGIMRGPEESAAGRATHGPGTHGGGHGSGGHESGGWGSKLRMLWTTVGRIAGFDTEDDHGSSSDAVGAGGSGPGPHPGLTHANSTRSGPQYNYTASHAAFDLEAHAGGGAAGGLGVAGGAGLPGIKTGLGLGCGSGGGSGQHGSSPYAPDGARRSLGLGAGASPFTPETGRMIAPEAMGMDTSSAGVGAAGARGPGGGGGGVLPNDPRIGGGAYQLSGGTAARERTRGSALPATYYSSVPTMQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Z6</accession>
    <name>A0ABQ5S0Z6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_006041</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666">
        <property type="entry name" value="PROTEIN VASCULAR ASSOCIATED DEATH 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666:SF1">
        <property type="entry name" value="PROTEIN VASCULAR ASSOCIATED DEATH 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VASt" evidence="4">
        <location>
            <begin position="99"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="52"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="472" mass="49815" checksum="C3903C3503AE654A" modified="2025-10-08" version="1">MATEHPGRNGQQKVHIRTKNIVDVVKGVAAAYVGLHLGIAALKFIGRRFRGKTGQNAGPKKQQRQQSSSSAASTQATITTTAEDSWAPVDTLAPTAPEGYKQIVTKTLPCRPQELYGLLFSSSGASFYLKQHREIGKQWDLVATSWRRASTAAPEPAREADADSGHVPDGSLLGRKSVFEWVLGQPGSGSFTRLLTFVNPKKPPNTVDTRCVQRQQFGVYQGGVLVFATAMSMLDIPFKDCFTVNSFWRVEPGVQPGTSTVTIHLKVNFIKRAMGVGGIVSATTMRESTAFFTAFINNVEAHIVAIRQQTASPLPAPLPLPAVATTAGSSQQGSRLPRSVSRNALVSPFAAVSGSLPPPAPAPAVTAGGHCGVSTHLQQPLPAWKQVRSLVVLAVLAFTVLHQLYLGRELAAIRGDSVAGSVAGARAGGSGDTSGLGGFVGGVVKPLQEPLVAAVAVMQALLDTILGRRLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2R2</accession>
    <name>A0ABQ5S2R2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-(3-amino-3-carboxypropyl)histidine synthase subunit 1</fullName>
            <ecNumber evidence="4">2.5.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Diphthamide biosynthesis protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphtheria toxin resistance protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_006617</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DPH1/DPH2 family. DPH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI63607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="434" mass="47083" checksum="AF7905CB80894D6D" modified="2025-10-08" version="1" fragment="single">RFSGAQAGTSDAIVSHSTANKSKDAGGAGSGAVRRFVRQQVPDDILYNDQLNKAIAVLPANYNFEIHKTVWRLRQAGARTVALQFPEGLQMYACAIADILETFAGVEHTLVMGDVTYGACCIDDFSASALGADFLVHYGHSCLVPVDVTGIPCLYVFVDIAVDVDHLVATIKLNFKDPGTRLVLAGTIQFASAVQLARQRLEPIFPSLAVPKCRPLSPGEVLGCTAPVVNPASTDAIVFVSDGRFHLEAIMIANPTIPAFRYDPYGRVFTRERYDHAGMRAARRRAIEVARGAQRWGVVLGTLGRQGNPAILATLERHLTEKELPYTLILMSEVSPPRLAIFPDLDAFVQIACPRLSIDWGEAFSRPTLTPYEALVALDVVPGWWEGAELQHGQQVGISIDGDAGNAGRAIAPYPMDYYAKDGGEWSSSYHKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S303</accession>
    <name>A0ABQ5S303_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="31597" checksum="17B3902F15D1F813" modified="2025-10-08" version="1" fragment="single">CPVTDLQRFQATLDRLWTAVRWENKNKETLWRLVVDGIPMPGNTHLSRVPIKPCGCGSYGADTDQPCSPRLHHFWECPIAKAVREQIDRHLPLPTSATIEGGLTRQQLWLVQAPPGCEQAPWDVIALAALSSMEYGRHCLRAATREHRPQWPSTHRRRQQQQTHTAEQGPRQRAITDFFNRSGGFPPEEEEDANTHPIISTTQPAMDTPTQTATSPLDRAQARTVADFWARLHSFAALGIPHYGWDQVGPQHPILAIVGTQMRCADPVDITNHGAARTEQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S316</accession>
    <name>A0ABQ5S316_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Patatin</fullName>
            <ecNumber evidence="5">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_007477</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4 5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4 5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="7">
        <location>
            <begin position="65"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="96"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="4">
        <location>
            <begin position="215"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="364" mass="37678" checksum="0C1CAF7D6871E5B3" modified="2025-10-08" version="1">MPAPLQAPNRVAGALGGQPQPFCLKCSLNHTRRQRSPDMAVARASPTRTGTRRAVAGPSVDACDLVLSSGFLAFAGHAGFLQAVEELGLPVGGVMGTSAGALAGSLFCAGYTPRQVARHLSDQTPASLLRPSLAPWRGGALSLDRVVSRLKDLLPPTFEDLHREFAVGVVTSDGRHLLIDSGPLPEAVAASAAIPFVFQAVDVPGHVARHGPFKDGGVVDRVGLKAWRDRRRAQICQSAKGPSSASRPPPPCLVHVIDRSSPFSGFDDTTAMGESSIVVIKSPRSGANFFDLGSFEAHMDAASNRARPLLAAFVRCNYSGGRRGSSGGGSGGGPAGTSNTVIVDEAAAAVAKSSPEAVVRARII</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S464</accession>
    <name>A0ABQ5S464_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_008050</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044807">
        <property type="entry name" value="DRIP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46293:SF14">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46293">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE DRIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="111"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="193"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="297" mass="32363" checksum="7FADABA9897B64B0" modified="2025-10-08" version="1">MSCNPTGGSLSPMPRLTQMSTRLGPPNRVTCVPTHVCHHLSVPATTLTNTTINSVTMAIFCYQIPCSFVHLFVRIELQLLIIVLMTHRSAFILYVSAQDRPAGPLASSLSCALCHELLQEAMTSLECGHSYCYDCIERRVDIGGNHNICPVEGCGVVLGPSPFDHHRLVYDTLLDGLMQKIFPRPDLDAALTTRRAEREEATRQARAAVNGCGGGQRNNQHNHHRQAHQQQHCSQQHQPTSARASREDGAMVAGGRRRRGGNSMDGGADGDDEGTARARPCTAFGEGIAWNRTNSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S465</accession>
    <name>A0ABQ5S465_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007221</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16482">
        <property type="entry name" value="RING-H2_UBR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF24">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="E3 ubiquitin-protein ligase UBR1-like winged-helix" evidence="4">
        <location>
            <begin position="509"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="E3 ubiquitin-protein ligase UBR-like C-terminal" evidence="3">
        <location>
            <begin position="2463"/>
            <end position="2564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="692"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="765"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="893"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="945"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1327"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1561"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1800"/>
            <end position="1852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2196"/>
            <end position="2231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2343"/>
            <end position="2372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="707"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="775"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="913"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="977"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1006"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1064"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1088"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1327"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1338"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1601"/>
            <end position="1611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1836"/>
            <end position="1852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="2204"/>
            <end position="2216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2586" mass="269267" checksum="D17398EF138AB155" modified="2025-10-08" version="1">MAVHLMDCYANMLSAIGGLAPSQQVPAGDGGDSSGSGRHPLCGVLDRLVVQLFNSEEVTTRLVSERNVMEQHLAVLSYLTGAFVDELHRVAGQGAAAASGGASLMDDWESDSASEQRLNAILDAMRRLASDFSTLVHHVPVAHYLLSNGQLWGEAFLGGFVRPLQAMLPHRRKVSGDVEERATFRVPLACSAEFVVTACVAELITTMTGLRPAAAAAAAAARSSGGGSSAPWGSRACSGGGTEEERVQRVKLLYGAARQAFEACEEWCSDRLARSGASGVASLTQGAATGGGSGHSFRRLSVAADLYEGLLAGELEASPHLPLHRTASAFVQALLHLEQEGGPMGQLARSHVEQLLSMQASLDEGSAWRPVNRFLLLGQAFVSQIIARRWLRNGDDASQVELFVLNGRELTDFDVLMTQCWMAAEDPDVVLYDTLSATGAKVLLAAAGVPAPGPSAGGGSSATQIAAPAAAPAELQDSASAAACSEALRWLVSLLRERSVTGLDPRERLRSTLVQWLALRDWTYNQLHELLPQDLQQQSHMEQVLAEVATYRAASLSQARHYSLRREAWDLFCPAFIHYTPSMRSRAWEAAMAAAKSFGGTAGSGGRGGGGDAVNVAYQPHWQLRRPRHPLPGSLEPMQRVLETLGMWRLVAWVLQACMSGAPRLPEDTLLGALNLLALQLHRLEDLLGRMSHANGHGKQTPPRAHQQQQQQQQQQQRDDSSKLTPTSGHSEELSRLRTSISVGAKALAGGVRPAAITGVSTHIHNQHEDPCDNQYDQDSNHQPGNNRRLLGATSPLGTGIVSLLAALLHSQTPTPASIPTPAFAGGPAPVAVQLSSETVDCLQWLHQTATRLVLEAEAAGVLVAMPRASDTAEAGTSAASTEAAEQAASDAIMCTGSGGGPASGTAEAAEAEAARRRDLARQRQAAMMAKLRAQQEAFLRRASVQDAGAVDSSDEDDDEECRAAAQSEEDEEEEEQDKHEEDMQEWRAAGSGAGPSTGGEAAVPSDEDSEDSEDSEDSEDVSPGEAGWVSSSLSDEGGDEGGHGGGVTRHGGRPSNGVGISGGRRRRGRHRGGSPGAAGAGAAASGPTQAAVGSTRQQLPLGRWLPVDLPGPECAVCREGTAGGRHGPLAFIAHTAMCFCTAAPLRPGRSWDADPATQSSEAMASLPAVAWPAATAAADKEQGDALADRQAPEDDLERAGCAAAGDLIKPGSAAREMWLQAWRRDGPAVCETDLGGGPHVHCCGHMIHTQCFARFVAGQLRQYLSGAHFPGSHLVMPEMSEFLCPVCRRLANGIVPAAPFQWRQPTAAAAAAAPLRRTACSQTLRARTRTRRKVRTHTAAGGNSAIAAASPVPSGSDLDASRGSGPHTCSRGISNSRQPPACDDDQSGPGDGCDGQVVEGVGCGSGATDAANAASEQDRGIEDWPMADAETAAGTEAGTESRAMQGPPFPPLPPLGHAAGLGALQGLMDSLELCTSGPKDTATVVEAGRSVAKGPAAQCVEPGRGPGLGNRGCVFERIFGIYGEVANMATHQIIREGITHMAQLERALEARARLLQLRNPPDAAPSASHNAALPPAAPGPQEDPSPGSIMEQQQQQHTQQRQEEAQDEHAATTQHPFLEPTTVLLARGSAANEGPYVSGQVVAEGQPAAATEAAATATAAAAATTSRSTWPWHWQVAVPVGLRLPPAPRPKLAGYCSRLTDALAVWKGLPPHTRLWAALAHNLAHFEAVRRPIVLARGVLGGPETAVATVGGVDGGSGTRVAHSVTGLPAVETAHWRAIVALTAMAVCGGVRHGSIGGDAAASGPAMPGERAGSGLPSRAAADAAAAHPQVGARRSQSNFQSHQHYPQQPNSQLRPMLAVFHNELRWLLCELQTMGVSRSLPPAPPQAPGMTTTGAGREQEQQLGAQQPSEFVGNAAGCTAELHEDLLEPPPPAASLDWTCAFLRPYLPEMVVPRLAASLNGSDPPSLRSRRSHPHKSTYLPALSFRRLTGLDDLTADPLLLTLELLGAGLSGLAGASLAPGAKVAASGAAAAATAELVEWLPGSPQQLLRTVLRLVWPIQVVQAGLWRAATANPEGADCAGAAASAPPRPASAPVATESGGEDLSLVSLVNLYGAALIELEAQQEQGQAQQSQGRVHDGVGAADSGGPGLALGSLSSALLPFLVRVYALDCALGRQTPDAALMSAYASLPLPLSSPTGQSRHNLHQGKQYRQRHSAAASGSAYTTPPLASTAVGPSARVEGAVPLAEALTHESAVLTSSTLVPDAVGLATMEMEVETPREAAALEEADLQAAARAAALVSHLVAALGLTGVVDALREPLLLSRRRASEVVVATASYTAGADKAATSPSAGGSGELPPHPHLHQSHASPPSDLEDALLARVLARWCGARILAVMVDPAAKAQNRQAGADPADPVGGATAAAATAAVAGAADVVALPARPQLTWYRGLPPPPSLIPLPYLFQPDNPALCLLTGRFVCNLWRGCGDGRGGAGGVGGGLLHAREHCGGACLLLSLRSTRVVAVRGNRLAMCQSPYLDAHGDEDPDLRRGRPLYLDAACYRQLSRLWGSAALEFDTQLLHNSRADLVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4H0</accession>
    <name>A0ABQ5S4H0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007568</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046222219" description="Peptidase M11 gametolysin domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="3">
        <location>
            <begin position="315"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="660"/>
            <end position="891"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="891" mass="92715" checksum="CAB011B29B399A45" modified="2025-10-08" version="1">MWRKALLTALLVTVAVHAAQAAPNTTTSSPNGKSSNAPGQASKTPSPSTTTPSPAPTITLSPPIATSPPATSNKPSPTAAPTPSPAPTITLSPPIATSPPATSNKPSPTAAPTPSPAPTITLSPPIATSPPATSPTAAPTPSPAAAGSPSPAAISSPGQYSNSPGQGQSSNSPGKSNAPGKTPNAPIEPEAPSQPPDAPAADNGPGQSSGSPGQSANAPGQIKKTLQGPLVIVTHELEETWALRNDDDGKITPMAKGFKPPKDKDKNKKEKDFEPGTVVAMNCNIDGGGTCTPTLDIPPTIVAAVPNYSPFIYQRVLVMILNYTDCGLFPGINETIVRSLFLGPNGDGLGGLAQKYTQCSYGRFNLNTTAFQVMVVPQSCSAAVTSSCSWWTISNDADTTAKTLLGTTAFSDFTHYIYVVPPGLNSVCPWAGLALLPGKQVWLQTSSYGVYRWATIMQEAIHNYGLWHSWQNGWEYEDYSTAMGRGDACPNAAEISRLGWATPAYGGAAIDSSVLTQPGVSRSFSLPATYITGDSNYLRVLPDWLPDYINSTLAKNLYLAVRVPKAGDAALGLQYANKLNIHEVNATMDNGYPTSYQYSDRKISFFKSITSLTQANLTTYQLSVYAGSWAGTDILRVHICRWLTSPDECPSLSSLEQRPGLPPAPPSPAPPPPNPPPPSPRPPNPPPSPRPPPSPRPPSPPPKPKPPPKPPAPPPKPPSPKPPPPSPDPLPPNFPPPDFPSPPPTSPPPFEPSPPAPAWPDAPSSPDPPSPEPLSPLAPSPDPASPPPPYPPPPSPPPPSPLPPSPPPPSPQPPEAPPPNPPPPSPPPPSPPPPSPPPPSPQPPTPPPPSPRPPRPPPPSPRPPRSPPRPPPSPRPPSPPRRPRSPPPTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4N9</accession>
    <name>A0ABQ5S4N9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="181" mass="18680" checksum="09D58C266A698F2C" modified="2025-10-08" version="1" fragment="single">LPSGAAAVPAAPPPPASSQPGFGPLVSVSSPDITCTTPALDATAVALAELAGATAAILDTYIQMGEAASLSATTMSELEEKYDYYDKSAMSAYVDMKNLATSALANASSGAQEILDLVDKTYQLETSNDDLIAAISMLMEVAAVSAGILSRTVLTSAFVLSSLEEAGLYNTTTLDWQQMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4Y1</accession>
    <name>A0ABQ5S4Y1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">superoxide dismutase</fullName>
            <ecNumber evidence="2">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008146</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045591698" description="superoxide dismutase" evidence="5">
        <location>
            <begin position="29"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="6">
        <location>
            <begin position="44"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="7">
        <location>
            <begin position="149"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="268" mass="28914" checksum="465B5F2A29AF9451" modified="2025-10-08" version="1">MGNLAAAAPAQGLALLLSLHLLLTSSYAQPPPPPSGKIGPARMFALEPLPYDYGALEPVISRRIMQLHHDYHDGVEYVSTLNGVLGPLAGSGTATLPDLIKMVGRGGLSPDEELVVRNFGGGHWNHQLFWKVMSPLGSSNTSRSNISPALQNAINGAFGSTDEMLRRFKLVGDGHFGSGWSWLCVSGDGSLRILDAANQDNPLMAVVLFDPCIPILGLDVWEHAFYLQYGPKKPDYTNAWLAIINWKQVSANYDFASRGDLAAIGTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S586</accession>
    <name>A0ABQ5S586_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007919</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF420">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP20-3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="93"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="251" mass="26765" checksum="61776AC114135DDB" modified="2025-10-08" version="1">MLAPSSGRCYGSRKAVSSTCMPSSIHTSTWRVIPRRAVVEVRATLASSGEPHSIVDRRVALAMGAVAAISVNGLAASNSVRAEEEPVVTQKVFFDVFVGGKPAGRIVLGLYGDVVPKTVANFVALATGEKGFGYKGCTFHRIIKNFVIQGGDFERGNGTGGYSIYGRRFPDENFKLLHAPGVLSMANAGPNTNGSQFFITTVDTPWLNGRHVVFGRVLEGMDIVHALEDVTVDRSARPTQPVVIDNCGLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5M9</accession>
    <name>A0ABQ5S5M9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase Y-family little finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008111</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025527">
        <property type="entry name" value="HUWE1/Rev1_UBM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14377">
        <property type="entry name" value="UBM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase Y-family little finger" evidence="3">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="439"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="537"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="443"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="464"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="739" mass="73490" checksum="B08D40D4E61EB6BC" modified="2025-10-08" version="1" fragment="single">GVRGRSLTLKLKRRQAGAPQPAKFMGHGACDNISRSVTLSRFTDDPTDLRREAVGLLRALNIPPEELRGLGITVAKLDNDSASLTTRPSAPAAAKLQAPPRTAFDASKPPPWAAYFQRKAQHHQQDHHQQHQQQPQQPQSPALAEQSAARAASPPPGHLTTPTKLPSAVVHPDPIPAAAPPSTTGTGPSLAGTARTAAKGGTRPSVPTSSGTSAGSPGSPAPPPPLAAAAAAAAAAVQQTRWQQEPEQEEQGRQQRYKGQRPAVATAGPAGRPMLPPPRPAVSGTHGGGGSSGGIGLLVAGRPRPPAAPAVSSASAGISFSAASADAGAAAAAHVIESIDLSVDHAEGNEEEEEYAGGGNGQVVGGGAAVQHPILELVEADAETSVGGGGGGSQHTVLAAAALAARPAVSETTTVGCPAVSNSVASGSAAARWPTSFLRPTVGSSSAVRGGSSTSGRGRGGRGRAAAGSSGRRGNAAGRISRSGAAAATGGLGSSSGARDQGPTEAMALQIDRSVLAELPPDVRREVEREYGLLTVAPTKRPPPATAQGHVRPAKSARDATVAAVRVQHAPTSERHSSDGGVPCSSTRGRGSAVGHEHQTNTNPMDAEPPKDTGGQSCAGCGPGLSGPSEVAATTAAMPTSMADVDLAVLDELPAELRSEITAALLRPSAGPMAAVVAAGRDAYYGGLGATTPAAMTAAAPPPLVQGPADGRAAPVIPVPKTGGAIVTAHVPLLGTLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5N5</accession>
    <name>A0ABQ5S5N5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="408" mass="46034" checksum="1263FAEDB5E6839B" modified="2025-10-08" version="1">MDVHHLDMRTAVLHGDLEEVIYVEQPPGFETGEPGQKCRLLKALYGLKQASHAWHKKLVATLEANKFEVSTSDPSLFSKRDGSRVVRLLVHVDDLLISSHCAKLLTEVKQQLMKEFEVRDLGEVSLFLGIEVVRDRGTRSMKILQKRYALDLVQKFGHANAKPDSIPMQPIRGLLNTRGIGDEEQEPFQEATQYRSLVGGLMYLAVCTRPDIAQAVRRLARYMSKPTPAHWEAARGVLRYVKGTAVYGITYSRGEPLFGYADADWASDRDNRRSTTGYVFILHGGAVSLKSRLQPTTAASSVEAEYMSVSSATREAMFLRKLMFDLGCPVEAVRIWDDNLGAINLIRNPTTSDRSKHIDVQHHFVREKERFGYVNFEYCPTESMVADMLTKPLPEAQFVKFRLSMGVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5T7</accession>
    <name>A0ABQ5S5T7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="178" mass="18981" checksum="47CCE6A032FF2E3E" modified="2025-10-08" version="1" fragment="single">MDDCSHAGFPRGFATRKDRRASLVIKQTAQLLTGLHEDASRSNALMAELEALLAASQVASGSTARKALTSANFIATQPLEISSEDDDCYVEEGEAEIKVVDDLSQPRLKAMHSIHRTASVVNISGEMQALCVQGTSASFSNRSLYQPTTTGASASPEGRRALSFRNGRASSYTLARSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6F4</accession>
    <name>A0ABQ5S6F4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="716"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="833"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="892"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="533"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="843"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="900"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="974"/>
            <end position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="992" mass="105110" checksum="7CDCB5A26D5663FB" modified="2025-10-08" version="1">MADPVLAHGPIDTAGEECLSPSTSTCTSAQPPMLIRPDESPFLPTCNPCTFHGYPDNLAAADGNDNIYLHKGGCGVNRSITYEVKIPIPVSCGISGEPLPPVAGMGGDMCGGNTGGGRQRTLKLYTLSSLKPSPSISVDLRGDDSDGLGSPLSCRFPWQGQPSQSARKLPQWQQHQQHLGQLHFAPQSPGCVSSRTAWGDPSGCDTSDLPRGRTGEPVGCVCVPSSACHLTTTAQPNIVDLRRPSTSFKSPAWGGYPSGPPIVPPLSVLHRVMQQTHQRAVKDEDVVCRDGNTKEGAEAMPVPCLWPSEHEGLCPTHADRGCLGQPRIIAAAGGTPQPQWSEMHTAEPDVRRTYGGSSADSRKRQWPQEQLQLPEEYTDLRLPGTFGLGLQMIHPLVSEQGAGALWEAAPQAIAVRDSAAETNVAIRANSQCHRVHAVPEAVMVMPSRWLLRPGLVPPAVDQDRAGVKICHGWAEDMDHSNADVVRTCLAVPRINGNWKELPAPAAAVARGSASGGGGFVGSEGAARGGGVAASGDLCTGDTTPSSTSTLPSPPPVGPSYPDQQQHDDQHGCAKRVCEVRGVQRLWTLRLYPLAARAMPPPAPPHLSPPVKVSARICHHFDDGHVLAAPGGTQPIHNWASTAVAPNPAAAASMGAATGTPREVPEVVLVRAMGSSSRGGSATVSSEESAGGAMEAINHLRGVRQDETFRLPLLGSISPPLMGPPTDPPQGEGTASPAVGLRPAMPPLHPQAPAARTARLKPDADTALQRDLAPSSEARFNELPKAGIQGIVCRGRVWCRERCQCGNISPQRCRGEELEVERCGAEVIDLTANVTEDENEDEKEDGRHHGKDGGNGRVVVRQSALSPREYMYVLPEGHGMSLVVLGGTCRKRKRSGARGVGGDKGESDVGDRNGPAAGGGTLHVRPVQKKRPHIEQAEIELAGTEDQQGEEERQEQKQGHKVSQSRCGWPWGRRGGPRKLLRGRGAHPRNSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6H5</accession>
    <name>A0ABQ5S6H5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_008451</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI65038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF453">
        <property type="entry name" value="ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="134"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="240"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI65038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="502" mass="53636" checksum="467DAC0FF8D86649" modified="2025-10-08" version="1" fragment="single">RVLRAPLSFFHTNPSGRIVNRFSKDQGQVDDLLPSCLFDALQSAFQVLGAFVLVAIAVPVILPVFLPLAAAFYWVRRRYITASRDIKRWEAVTRSPIFASFSATLKGLPTIRAYGAASRFDDAFLKLMAHNGNWYFAFISTARWIGVRLDAVAGTALLAAALLAVAMRDSINTGVMALALTHVLQLTGLMQWVVRQTAEVENTMTSVERMLSYTELPSEAPRVSEGGGSPPPGWPKSGALTYEHVTAVYRPGLPPVLRDLSFSLSPGSSCGVVGRTGSGKSSLMLTLFRLIDVVHGRILLDGVDTAAVGLDALRRQLAIIPQDPVLFSGTLRSNLDPWRAHGDEALWEVLQAVQLRGAVAAMPGGLDAAMAECGGNLSVGQRQLFCLARALLQDAKVLALDEATANVDRATDQLIQSALRDFAHRGRETQGRVLLLIAHRLDTILDTDQLLVLAAGQLVESGRPGDLIAQGEGVFAGMAAAAKLAGAAQAVVVVTGAAESIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6P1</accession>
    <name>A0ABQ5S6P1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="266" mass="27470" checksum="738F0463688F4AF5" modified="2025-10-08" version="1" fragment="single">MMTCFGLCRRAKGRKPSQGESDSTVCAALALPLSLKESATAQTRPAASSVEFQGIPFRSTQEWLQLLCTLQREIGDLQSNPMEGRASNLVNMLLTSLSASAVRLYCLSDERHRGGSAALVLLAAASSPSAATGMNSPDEGCLGQSLELPLDTALSPISPQRLTNAAASARSHKESPPSTADLPTLLHRAITSMEPALHPAPPVTLASGGGGGGGDDSGVGGVTAKAAVAAPEVDSVMRINALPGDCLYDYTTHESTKFVALPLRCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S758</accession>
    <name>A0ABQ5S758_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="414" mass="47105" checksum="ED885F939FC239E5" modified="2025-10-08" version="1">MAPLGVRYKLPIGNKKLQQLAYKAKLPWFQRRVTSAWRLQSRLEVEVGTLKTVIEALQASSEEALAAADQRTEVLQLQMAALQQQTQLAEANVRVLKAEHEQQVSMLTSHLRSAEQVAQQERSQWRVKEEVIRTDAQARVCTAEAKAACAHSKLKTEILTTEKFRQEVQAAMEAATMDAAKSAQLAMDLQAAKSEIEVLHKESREWKAREEDMCSKLRAFARRNEQEGQEFRVELENKDFELDFLRAKMARQEKIVQDTALRVQAMEKDNCAMKENLTEEIGRLKHLVTCTEKRAADATADYDGAMRMVARLKLQLSEVWAGKASAWSIRAEATAENVELRAQLVEAKVTIARERSLARVDRREEIRCLKLELRNVNESWSRRCEELKDSLWRREAQAAGRTGTAACSRPTSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7H8</accession>
    <name>A0ABQ5S7H8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009519</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="796"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1099" mass="108999" checksum="954403420E69D0DA" modified="2025-10-08" version="1" fragment="single">AGAAGAGGISMQASVPKADNLIPGTSLREEMYGSLEAAAVTLSAVVEVCLPPKEDLQLSTQVRVTYKVPLSAEGTVRFTSDCSASGTGSGTSSGTGTGSGTGSGSRSAASGSPGTVDSAGGSAALQGAVQCAVLPAPTSVGQLNEQARRDIPPPPGSISTQAIATSNAKSRSIIMTVGAAVAAAVVASIVLGVVIAMRRRRRHLEREVQEMAVDTALALTQPSYIDVYGALAPLPTTRSSTAAGDLTTRFSRHQQGRSFIRRAAVLLEQVAAATAREKSASDGGAGGDGGGAGGGGRRGSLSRNQFERPAYASVSSRTHSHSRSRSSSMTPARGRALALFELAVPPAGGAASAGGSGSMTAGASTTSGGNRSRRLAAGASRFRAAALAARFAVADHTGMLSGISESAVDGSSGASAAAMAAASGGVSLTTAAAAAALLGGRSRSTPGPGMTRLRDGVHPELGEASVLSAMTLREQIATARAAAIALLAGGSGAGGGSGGSGSCSSGGGISGGGGGMNRDSTVRQLAAVATHGRCLTPGRVSTHSALARKSMRVSIGAVASASASGTAVAGAVRPSHTGLPLHAERSSGGGGGSCGGASCGGGGSCSGGRTCVRVGPEPGLGAANELEGGGSAADGAAGPYFGPGRLPASCTPSHQSCQFGQRNGGVHVSVFAAMQSNNDQLDAAEVGPLLPGSAERTVGRLLESHPEICPRSSYPQYPLPPALRLSSPIVRLSACAGQTTSPQIRVQMDAPAGTAFTQLRQRQTLRPELDPSDAPATAAAAAAAAATATTTATTAASTVQNTASASRSVSVSVSSGSSGTTGVLTQLREVGAQIADTPMVVVAAAAAASVAETHGSVHEAPPAPSFIQDQAIRRATTLQQWLQPRQVTELWGHQAGHASPPAADANASVSTCGRDLDFIPELRERATWASPRTESASGSGLGFHSARGRRRYTMATASLVAPEPGALEPEGGELAATAISASTHFGALSRGRSHRLSSIGRNAVTRLSGADGVAIPAAAMPTSLIAPVVHVESEVYGWEGLPSMPPPPPPPVVTQSVTTPHSAPLPVPAVLLLPAVLPNRPDAENEDEGGADMMCFQPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7Z7</accession>
    <name>A0ABQ5S7Z7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009371</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007657">
        <property type="entry name" value="Glycosyltransferase_61"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961:SF38">
        <property type="entry name" value="PROTEIN O-LINKED-MANNOSE BETA-1,4-N-ACETYLGLUCOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046338965" evidence="2">
        <location>
            <begin position="22"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="763" mass="83056" checksum="448137DB654B72B2" modified="2025-10-08" version="1">MQSCALYLVLLLVSVPVQISCQKKLDKKWITSQRNMSWSLQLSQPSAFVTRAQYRLRKRAGGFKPSTTCSNNLGLPYLENIRRSRYSICGSTILTQPDKNTLCTQQSACQSAVDCFTAPLALDANSSTGSISLCRSQNVVLDSCKFLQNEREGILSKSFPHPKPGSVKVACTLVNMTSAAKLNNKLHWLRNIHHEVWWGNATQDSPAVFQACSPNSSTLVTTPTMFVMRGRHTSYAHEVELVSMAFSFLAALDPEDVARHGVQVVIADRFPVTSFLETWARVSRPHQLRILVSDPFPPNTCFRSAFHVYASTGGIGYNANPDTVRCESAVVTGFSHWLRQLYGEGDPSGRLVATPPSAEAVAGPAAEEAGIRGLRPPAGGIVLKNVVWLSRRHMELVRLLLNASTGWISMRMVRNEAAVVAGVLSAVQEWNAESCLLQRFDRTIKSEYELSLTNVNPVHGGATNSARQTLNEADSTAVAGHARSLTGLSRQREQQQQRSQQHHSHPKRKQKEGFISVDGMGIWHRRQMLQDASSGGCSSGGAGGGSPDDGGSGDGEGCNSVEGKSRDGSDGDVNTEGGNVGGSSQSYTIGNNMIVLDKLWEMDNSINDGCRRTNVLFKFVEGGFSDDETFNMQLQTIFRTGVLVGVHGAGLAHGFFMPPGQSAVLQLLGKPFSKVSAGNIFRNMAASLGNYYEDVLYSDVDIDVAVLKAAVKRAMDFVARKVMEAQLRRNGALRLVLVDQSHFSIVLPSEEQCPANATLWASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8H2</accession>
    <name>A0ABQ5S8H2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Copper homeostasis protein cutC homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009506</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.380">
        <property type="entry name" value="Copper homeostasis (CutC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00795">
        <property type="entry name" value="CutC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005627">
        <property type="entry name" value="CutC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036822">
        <property type="entry name" value="CutC-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12598">
        <property type="entry name" value="COPPER HOMEOSTASIS PROTEIN CUTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12598:SF0">
        <property type="entry name" value="COPPER HOMEOSTASIS PROTEIN CUTC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03932">
        <property type="entry name" value="CutC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110395">
        <property type="entry name" value="CutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="246" mass="26153" checksum="F73C7E9C6A71E5C8" modified="2025-10-08" version="1">MLLEVCIDNVHGALAAQSGGADRVELCAALVEGGITPSFGLITACRKAFQGKLMVIIRPRGGDFLYTEEEELVMLEDIRQAKVCRADGVVFGCLQSDGTIGKGATSRLLRQAQILDLDVTFHRAFDMTPDPVQALDDLIAVGVPRVLTSGCHPTALQGADAIAGLVHRSAGRITILAGGGVTPANAAELVRHTGVTEIHSSARSRHISNMSYRRPGLSMASSQPPNDYEWNIADADIVRQLVKAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8I1</accession>
    <name>A0ABQ5S8I1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009436</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045945508" description="Pherophorin domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="705"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="673"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="738"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="784"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="499"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="755"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="840" mass="86962" checksum="B64D86C5819D7513" modified="2025-10-08" version="1" fragment="single">MTRRFSGSAVLLALVLILVPAVRSARISNQALYLTNIRFGATPTVDGVYDVEFSVDLTSPVQHVKLEVEVGGFHVQRFVRCPCSQTQVHGVIKASTLSPGEQDIVITARAGDEHPTVIRLKQDIGEMLARTEADFGGRSRQLGQVSTDIPDSPTRFILDPWMVPSYPPPDYDYSLPPPPDYDHYVPFPPLYSQAPDAPPERPDDPPPPPPPPPPPPPSSPPPPPPPPVSPPPPPPPPPPPPVPPPPPPPPPPPPPLSPPPPPPPPPPPRESPAPPPPVQPLQRPPPPSPHSLRSPSPPPPVPRPPLRYRSPPSPAPEPPSPRPRRPPRPPPSPPPPEPPSPRPPRPPRPPPSPPPPSPPEPPSPRPPRPPRPPPPPSPPEPPSPRPPPSPRPMPPSPRPPPSPRPMPPSPPPRTPSPPVPPPFPPPSPLPPHPLPPPPHTPAPLSPPPLSLPPPPLSPPPPSPLLPPPPIPSSPPPLPTAAPLRPASRSSPPQPPHPSNPKLSPGSPTTAPSPATPLPLSSPPSSPLVSSPSPADVPEGFSTSPPSPPPPLPSPPLPSPPPPVSLTLELRLVGADYDAIVGSPEALSALKERICAELLASTRAISCTIVDVRRGSIIFAADLVPSPGTSLNAFANQLTASQPTSLFSSSFLGTYGITDVDSLVIRQLPPAAFPASSPAPPPGSQFQGSPVQPRGSSNRVLSTRQIIILAVVAPVGFVILVSTVAIIAVVIHTRRKRAGMYQTGEGKDSEARDPEGGTSARPASRSASGAAVAVAVAAADATARAPTSAPSLPYTRAPASVPAPMSAPLLAPKPVRAPASAAAPTPAPAPTSSAAPKSALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S903</accession>
    <name>A0ABQ5S903_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="1">
        <location>
            <begin position="120"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="260" mass="28546" checksum="F3182D7BF3D2B870" modified="2025-10-08" version="1">MLLWQVVSTFRTSLHLFPVSNHLTPGAVDIPGMAPAAAATTCAAVAGTSSRPPRPPHTGHEDEVIKIAIAVPKLNIPMDVKLSDLGRVAAPLTRAFVRNVRNYFVTARGGQQDSTRRLFLATAFEGAARLWHDQWTNARDSYTSEELLEALLVRFAPQVQSRDTEARLKLNAGKYRMRPGETVSAYQSRFEALVTPIADLSDSERVFWFQQGLSSDLSGDCATDLSGKTFKSYTDLVQFSLGAELRLLAGRESDKLAVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9W7</accession>
    <name>A0ABQ5S9W7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34407">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34407:SF1">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045907430" description="SGNH hydrolase-type esterase domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="541" mass="60380" checksum="201A9A872E868DDF" modified="2025-10-08" version="1">MGCSLDWPKWSIVVLLCFCSVPVRSLSEKSFGTDFMGRTKNRPELEWDWGHTWSKVEVDKQPVNVLLSHVLGTYKFTLPRTQLQRGTAYVGSSVRLRRLVREMLNPQNRKDFKIAVIGGSISWGQSTSERGVTDWFSVLSKWMISAFPRANITARNGCTPGVPTPYMIMCLELSVDTDVDLVFMEYTLNDGIDPMLFNNRIVMDMERLVRRVMDLPNHPAVVFMHVPTFGMANYPKGHEKNPGNETYTRFYETTEDAQTAISQYYDVQYLSLRTAMYRLAVHKGLEGFRWEDAFVDHHPGDHGHKIMADLAVNLIQRVTLGLLMEPYSAADMEAANEPLPPPMYEGNTAPSSPMCLVGDVFRQLVVLSEGFDYINEGTAIKPKPGFVSTQPGSRLQLRVDTDRSAVGSPPDGMVHVYLHHLRSYEHMGIAAISCISGCSCPAVEVNAHITEKVSQLYMARLVISQSKDCIVEVKVSSSTSSGEHKFKVSGLVVAERAGVGDVMERLGGDNQPFGLRQHNGDATQVVWTKEGRTGGHGQPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9Z7</accession>
    <name>A0ABQ5S9Z7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="162" mass="17877" checksum="0927489C2CC0D4A4" modified="2025-10-08" version="1" fragment="single">SSEVFDGYSDSDWAGDMDTRRSTTGYVFRVNGTAVSWSSQLQRTVAASSVEAEYQALSAAVREALWLLKLCEDLGVEASQMRIQVDSQGAMNLGNNPITSPRSKHIDVQHHLVRERVASGEVALKYCCTEKMVADALTKALPEAKFTWCRREMGLDVVETKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAB6</accession>
    <name>A0ABQ5SAB6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_010170</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Na(+)(in) + H(+)(out) = Na(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF197">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="12">
        <location>
            <begin position="43"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI66379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="321" mass="33145" checksum="CE788DC09B7E3698" modified="2025-10-08" version="1" fragment="single">MTPDEALGAVAGVKIPSKPTEADLEVAGLTTGVLVLLAALWSLLGHYLSSRSRYLNESGTACLLGLVAGMALLAWKDTSNAGLVNGLLRFNSAAFFTYLLPPVIFYAGLSVEQRRFFSNLPSILSFGVLGTLLSFVLMSAALYSLGGTTFGLDAQDCLALGAVFAATDSVAVLGLLDPRTAPQLFSLVFGEGIVNDATSVVLLGALARMARQQEGEPGGGGEEAVLVLAGRLTWNFGYLLLTSTLLGFVVGVCVAALLRGRAASEGPHHEVALVGLLSYLAYLAAEVLGLSAILSLFCCGATVSHFALRNGAGGADEDRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBC8</accession>
    <name>A0ABQ5SBC8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011429</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040441">
        <property type="entry name" value="CFA20/CFAP20DC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007714">
        <property type="entry name" value="CFA20_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12458">
        <property type="entry name" value="ORF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05018">
        <property type="entry name" value="CFA20_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="190" mass="22196" checksum="11239FEDCC71F22A" modified="2026-01-28" version="1">MFKNAFQSGFLSVLYSIGSKPLEIWDKQVSNGHIKRITDTDIQSSVLEIMGQNVSTTYITCPADPNKTLGIKLPFLVLIIKNLNKYFSFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDSGWNQIQFNLSDFTRRAYGTNYIETLRVQVHANCRIRRIYFSDRLYSEEELPAEFKLFLPIQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBE3</accession>
    <name>A0ABQ5SBE3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="110" mass="12293" checksum="E1D7BEE09F091B0A" modified="2025-10-08" version="1">MESAQPRRDIVKDPVTNVAAVGSNSDNGSHHDREDAGDLPQIKAARSSIPKDNPDTQKLLKFIEDKYVTLKMEYAIQKWRMPSLPSFPPDWSIDVRAHSGQSARSMNCIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBH1</accession>
    <name>A0ABQ5SBH1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011097</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI66999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01005">
        <property type="entry name" value="PBP2_CysP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005669">
        <property type="entry name" value="Thiosulph/SO4-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00971">
        <property type="entry name" value="3a0106s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30368">
        <property type="entry name" value="SULFATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30368:SF2">
        <property type="entry name" value="SULFATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13531">
        <property type="entry name" value="SBP_bac_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="439" mass="48230" checksum="BBE8C0C9D68C44B5" modified="2025-10-08" version="1">MSFFISNTISHGRCPEAGENAHSSRTTSGLQILRICAPMNASIARILQNLGMASDASASEHREQRMPLRPVQFATMAALLPWQPPPGDRGGGGGGNLGPNPLYNLALQPPQEVVLTLASFAVTKLAYVRVTKAFRELYMRESGIDVRFRLTFAASGVQARAVIDGLPADIVALALPLDLDKIVSAGIIYPDWRTRYPCNSIVCETTVAFVVRQGNPKNIRIWDDLTRPDVEVVLANPKTAGVARWIFLALWGAKMRHGHAAALNYVTKVFENVVVQPRDAREASDVFYKQKVGDVLLTYENEVILTNEVYGDKALPYLVPSYNIRIECPLALVDKVVENRGPDVQKAATTFCRFLFTAAAQSEFAKLGFRINPRTCKEVAVQQTGLPPAQLWQVDKELGGWAKAQEEFFDAGAILDKIQSDVGRKRVLKQQAAAAAGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBH8</accession>
    <name>A0ABQ5SBH8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="106"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="176" mass="20025" checksum="95F53D6D3535CBD3" modified="2025-10-08" version="1" fragment="single">SLLIRTQCIIWVVITALSYKAFHCFCVLRMNISQVERIVCDAFNAFSSGEPYLNRHSFKCAVLFATGWKPRSQDLEACLGQQVGANAMLTIDHLQRVVQMVLSTRDSTSRLREVYQAFDTAGLGFIRREEVLHIFENVAPTVRTNIVEEVFDALDTRRTGRVSCEDFMAELRGRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC51</accession>
    <name>A0ABQ5SC51_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hflx-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="entry name" value="GTPase HflX, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="GTP-BINDING PROTEIN HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229:SF8">
        <property type="entry name" value="GTPASE HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="2">
        <location>
            <begin position="489"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="646"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="716"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="804"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="754"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="820"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1271" mass="133189" checksum="9663677B5C608F4B" modified="2025-10-08" version="1">MTPESAPWLLLVGGQRSATYRFLYFSQRISSTLVRSVLRRWDVLDPWTRLIFGEAVQRKRPSQLFPQPSLVNSPSFILQSPEHYTLPSPRHPQMFQLLSLPPLQPTPWLVSTSRSISSGMGVVRDATLVTAPSLATGDLVADAGPASLPTLLLVHPRYRGVEDRKEALRLAESLVGRPCPFLEVGGSRLRNRRPREVSSSPAFATALRRPVVSGLRATYFGSGTMVDLAQRLDELETTAESGSGSGSRTSSVSARQRQQRDLDRGKEDGTGEGSGGGTEAYRNIAGDGGHGRDGRHVNDGDNPFMRGGAASGSGSGSGGSAGGSAGGSGSGYRATAFINEVLTPLQERNIEVGLNRPVMDRVGLILRLFAQRAHTREARLQVELASLSYMLPRLVRVRGSDGRREAFGIGLGWGGLNRDLGPALQVVSARQRGTTGAGGLGGGGGSGDPELRQQRFRIKQRLAALRAELKAVAASRGVQRQGRRAAGLPTVAIVGYTNVGKTSLAGAMCSKDAGLPPPTDMLFATLDPAVRRAWLPTYGSHVAVSDTVGFIRDLPVGLVAAFRATLEEVVAADMIVHVLDASSPNVAQQRDSVLAVLQQLGISEHVLTCRTVEVWNKVDLLTCQPGHQGTARNHNVQQLEDVRRKYGAADAGDSDGGSASGAAAAAAGRNADQTGCEAADVWHKEADKPGEEGEEEGEGVEGRFRGFRSFAAMCNVDSDDGDDTTERYSPPYGGLRGGGTTTVVGGKQQPGWDEQVDLTDDADGGGYDISGDELGGKEAAAAAAAPFLQRPTASGRFTRRLFPQETQQGRGEPSAPAPAGPRADGGVAQPRPQPQPQQQPGRTPSDGELSASPIPIRTQPPSPSSPHLAEGKLSTRGFCYTAAAAAASAAVMAPPSAPSPPELGICYYKRERRNLHSAADKYGNGSGTMVGSEIGSEAEATVREYDAATAGEERGGAEANWLNPKSLAAAADLVRLVQGSGSCVPAAVVLTAVAEDGGGVEEVKAAVDAVLRTISGVPPQQHAATQSRTRVGCQAIEEAAAAAADGSVSREPPVAALAGSGTTTGRWVTPLLWLADGVLWPLPPPPPPSASYSTSKRWRGVTRSELLWGCRQRGAPFSGTAVKGVKGADVGGNEAAKSPSSISGRGTDLLQAAAAAAAAAASEAEAGDWSMTSSRLSFSSWHERASFPEADVDVDATGRGDSEPPVARVRWPQQAAVRYDAQLPDPAAAAAASDGALKKAAVTAAGLPDDEELEELIHFLRRRRPRRENDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCC1</accession>
    <name>A0ABQ5SCC1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45134">
        <property type="entry name" value="OS08G0543275 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45134:SF5">
        <property type="entry name" value="OS08G0543275 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="133" mass="16433" checksum="31E8ACACBC5324EB" modified="2025-10-08" version="1" fragment="single">YTHTYTHTYTYAYTHTYTHTYTYAYTHTYTHIHIHTHIRIHTHIHTCAYTHTHMRIHTHTHTYTNTHTYTHTHTHTHTHTHTHSHTHTYTHTHTHTYTHIHTHTHTHTYAYTHTYAYTHTYTYTFTYTHIHKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD45</accession>
    <name>A0ABQ5SD45_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="51250" checksum="D7AD7D6170C7BA6B" modified="2025-10-08" version="1" fragment="single">MVKENTTLASILTTCVAQQPAPPSCIVSPTGAPPTASDTAAMRHHRITTTAMGYALPYTYFSMYNPRACGALDKDEQGCSPGGLLDIQICMVNERRLSVLHVPTVATVLGVLMVIVTAAVWQRKARRASGRSPEGISSTLPLLPLWAPSPSSQHRRHRQRQQQQQRQSPACRRSLWPSPSQSPSAPSLPTPSLRASSLPAPSLPAPSLPASSLPAPSLPAPSLRDPSLPAPSLPAPSLPDPSLPAPSLPAPSLPAPSLPAPSLPDPSLPDPSLPASSLPAPSLPDPSLPAPSLPAPSLPAPSLRDPSLPAPSLPAPSLPAPSLPAPSLPAPSLPAPSLPAPSLPATSLPAPSPVTSSPPALSLHSLSLPGPSLPTRLSQPSPPLPLPRSLPSSPPPPPCTPPTQLWTARGLLPPPCPLPPVTASTLRPPLPPPPGNIRRPAELLRGEDGEDEGRQFSGLGPIVDQPPRRRVSQGSGIQGPAGAPRSQEEKRNMAVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE88</accession>
    <name>A0ABQ5SE88_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="130" mass="15255" checksum="D2BE115110161DB1" modified="2025-10-08" version="1">MAFILEYLSYVWTKNNDDAASYKEWTDFLDVAEKDRRRDRIISWRERVMYDDYAVNAEEPIVYKRTAGFVWREVDHTGKHKNHNHTYMYNLQTFGHRVYSRRDPAELAAAHAVVADRVTKGDLGGLSPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEU0</accession>
    <name>A0ABQ5SEU0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">VaNZ11_012511</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021319">
        <property type="entry name" value="DUF2921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF162">
        <property type="entry name" value="TRANSMEMBRANE E3 UBIQUITIN-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11145">
        <property type="entry name" value="DUF2921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047206862" description="RING-type E3 ubiquitin transferase" evidence="17">
        <location>
            <begin position="33"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="615"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="680"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="729"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="759"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="918"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="42"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="334"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="286"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GLI68171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="967" mass="104229" checksum="0C971C87AC4518A1" modified="2025-10-08" version="1">MDRGRGRELEWPAMASMQLLYILALLLQLAFGSTYANQSQHISSKPLHDNSSTDGASNVVGRSDDLPNAVGSSRDYRSKATFSTEPSSRLKSLRSGPMVPPPVPSSWELELAPYLLDMSPPRDPNTTLLRGPPRLVGVFKGTWIRAAYGKALQPQDLLSSEQGVIVLRLPEEPLHSAVYDDSGVRAERLQELRGEMVIRDGDSVTDHDLRFRIKGTYVAAAGTVHAVLEPHPPWMLKAELENKQFSDGAGRGDSQELGGTGSGQWQLGDTATVAAATSGREAFAGGSRGTGSGVRGGGTGAEKVGDAESSGYREALRTAARNLVLLGPAWHRTLVRASPPRPPSPGRATSRRRRAGEDSEQLSLPPSSQLSPRPPLELALMQNCELRADFRVYYKGDPEAKSHVQFLGLDQPPSPTRAPSLKPTAMALSPSITELSGGDGGATTGEPVRLPSVAAATRPASVNLNAAPVRQLRRLAADTEIYHSTQMQLHADTTAVAATAVGSGDGAAVGGVTAAQDSVYGIPAGMDPLDPDLELIGIVVSPNCEFTLHFNASSIHLERYYRQAVRYSGLVATVTIAQIVLSVSQSEAASTPSAAARMSLYSVTLQAILDAYQCLLHLTGALVVDALFAAFASIAFLQFLLFAVFEMRQTLLVWRAQRVNGNGGDFWTVRRDMSAVYFRFYGLLLLGLLLMFQFRSHLALMVLLLHSWWLPQLIHSARSDTRPPLKAEYVWGMSALRLVSPLYFYGCPSNLLRVEQHSGVCVALVVWVGLQAALVIAQIQLGPRVFIPRIFLPQLYDYHRPATKREMGIEEPQSASAAMPGTLSASLLASSAEAMTGSGWWHWVNPSGRPALLRRLTSWLTSAFAWFRRPSGFSAGHSVAAAAAAVLQSAAGAGGRCGGEGTGPDDADVETGHGSKECVICMNPVPLLPFNARMLTPCGHFFHERCLTRWMEVKMDCPTCRRPLPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEW1</accession>
    <name>A0ABQ5SEW1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_012882</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF79">
        <property type="entry name" value="PROTEIN SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="146"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI68460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="573" mass="59757" checksum="95E431B45991C37B" modified="2025-10-08" version="1" fragment="single">MAGGGHSSVQDAREQLVPKRDAEVEQSHSATHVFPQYDLPLPSTSGVEKLSEVVDPIRNGYRARPEEASGSSKGAVTDGPTPVGVNGAPPPGSVPAPDLLTSPRPPMPSAGGSSIVAAMEIQEALLPAQPAVPFEHPISLPRRRWMVFMFCLVAALLFADQNLLAPNLTAAANYFGMNERQKDTLLGGALMAAFFAVGAPAALLVGWLADRGDINRRTLLFWVVVVGETPCLLTYWVASFWQFFLLRALTGVAVGGCFPLVFSLLGDLVPQSRRAGVSAVVQIATGIGLAAGQALSGSIGPATNWRVPFMVVAAPSLAVALVMLLTTVEPPRGAFEEALRERYAEGMGSYEGSISWPKVAVLLRCPSNVLVIGQGLPGSLPWGMVLTYFNDFLAQQKGFTVQAATVVLLFFASGGAVGVIGGGAGGQWLYNKRKEYQALLAGGSVLAGIGPLYALINADLQHIGLAATCFLAALGGAVASVAGPNLRSVMLNVNEPETRGVALALQSMTDDLGKGLGPVLVAGLIHALGRKGAFNVATGGWIPCGVLLASLACCMRRDEDAMQARLAQSSEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG60</accession>
    <name>A0ABQ5SG60_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="108" mass="11852" checksum="693C422A1797D87F" modified="2025-10-08" version="1">MSGRPPPALLALAASQPPGDKAAEQLAQPSSSNETQDTIGQPEPALSAPPRSILKTAFSLRSNDGYQRRISWPDHYGKSLTQVVEYEPSEHADSEYEDFEDRSCCMLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGB7</accession>
    <name>A0ABQ5SGB7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="218" mass="23148" checksum="FD166A3916EA8BB8" modified="2025-10-08" version="1">MPGLSKSKHRFPYSTLSHRPAMGFFIAIRQGAVQQAHGGGRRLTSLVPGETRSCPSFPRTVVSEKNWSVVKWGHCRWRSSKAALGSSEPLAIAIAGAFVGSGSNCWGGGGGGGGGGEDVDGGIIRRGNDGGDGDDGAPASSAAAAAARRLPNTKGWWLEDRGLRIEKQAGGCASETRKSCERHGKTHISTANERIPKQKQCKIAISFQMCYHVKMRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGL7</accession>
    <name>A0ABQ5SGL7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013081</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="268"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="421" mass="45051" checksum="136BD799190A6FB8" modified="2025-10-08" version="1">MKGPQQQEEILHVGFNQDYGCFSCGTTKGFRVYNCEPFKETFCRGFNNGGIGIVEMLFRCNILAIVGGGAAPRYPPTKVMIWDDHQGKCIGEMTFRSQVRAVRLRRDRIVVALEHKVLVYNFADLKLLHQTETCANPRGLVAISSTAAMSSASDNTVLACPGLHTGQVRVELYDRRQTKFISAHTNALSCLILSMDGKRLVTASEKGTLVRVWNTADGQSLQELRRGADPAHIYSLALSRNCEWLALSSDKGTVHVFALGPAVALTSSGSAAPASTPSGAKSGGASGDEAQRGAAPPEGVSIANNGGDNGCAVEAASPMTPTPRQNPTSFLGIVKGYVPAIPLPKYFNSEWSFAQFRIHDEPASNAGAQQPSIVGFGAEPNTVLVVTAGGSFYRVVFDPVKGGQCTQLSYCKFLEQQDAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGN6</accession>
    <name>A0ABQ5SGN6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyl transferase 48 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013564</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF48">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1770"/>
            <end position="1794"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1843"/>
            <end position="1861"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1906"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1930"/>
            <end position="1952"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 48" evidence="3">
        <location>
            <begin position="1153"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 48" evidence="3">
        <location>
            <begin position="1516"/>
            <end position="1987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="965"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1014"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1432"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="287"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1989"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1989" mass="212974" checksum="E2765F1246B98AD9" modified="2025-10-08" version="1" fragment="single">MALFGILRGLLRLDLGTVASWEELRAAFHRGPVRWWRRCMSAEGVSSHRRALRRHLMEGGQDRAAVEAFVPETPAAQNNISSDEDSDDGAGNSGGSADGALSNDDMEDHSGDVERLSSAATMPSSMSRLMWPLRTLGRGARWARERRALGRIRENGGASAMAMMELKDGDESQNFMLQTAERWATVPALPPLMGSSFISSRRRAGGGGTGMIAPGSTRAGSNSARWEALEPLQRRRGPIRRPSIPGASMERSRRRFSEGGATRAVAEGLEKADRSDAGANGRRGCNSSTAGPGTASAADDGRNGLWSRSGGGAAGAAAFISKGSSNGNARRPRISGSDGEVERRLVGTYSPVIRNPAKAPDVSGLWQRLSRRSTPSGAAGGASCGSVSGAAAPTRALGIFIRHNAVAPLPLSPPVAEVPALPPDSPPGTRRVASVGGAAGATFGGVECLVTPRRPTFAVPQIPAGPVAEATSTPSTILGLTRLTRREAASLHEQTPTTMHATRRNPAADGWRQRDQNYWQQQQRQLLSPAAASPSARTPIPQASAAPPMRQATSPRVDQPQLLTRASLGSLSNLSSDPDDPEAARGETPAARVRDEAVASVTAPTAVPSAKVGAEAIYKRPSQRPPGFGSETTERDRVMALQSTEDLSLGVLLSRQNGDLAMWDAFATVWDGIVDDLRATDLISDRESGNLRFMRLGHFQGRHALRPILLPAFFYAGVVQAFVDTGRLTSGGGGGDGATTTILTELRSLVVWLSCELRLLSEVQAEVVMGISFLHTITDVDHANYRAKMLHAGQALVKQLAELCASAAKQQQLLLQQPREMQRQQRQRPDWHQVQAAGGQVHFENGAVAGGKFLNVTSDARMGRATSQRQAGTAAAKTLVALHVGAIGGSLREVLEALAGEARAMRRAVASGRLASESLAAAVKLEEVAQQVLADLSAHPDFLSSCLTNLLPRAIFVCPPRATYAEDDEPESPTATGGPPSAKPTASYIQTPQPIPSNFVGGNLTTTEVPLIHLGSPSSARSTEPRGIANGGASSSPLHHRPPAQGATAITTMFPDSPNPSISGAVVSDVATAGAGAVTSLPRAPSLARVKASEDVFSKGMRKGVRSGSSWGSCSLESVAPPTLEPEVVLRVRVVEVLVKMLTTPASACRPAGAEALRILGFFINSLSNPQLKKPPPLGDMLSWSVLTPCYEEDVLYPLSADHAARQLGLAPPPLVGPGRPPDLLSETEDNVSLMSYLRSVFPADWKNFMERLSGMLGGADLSRVTENDFAPMGPLHDLAAELQLWATYRGQLLGRTVRGMMCYRRAVRMLVELEYPRPAGVSPEGYNAWAEAFVDCKFQYVCTCQVYGKNRKATDIRRRWLAEGVDSLCLEFPALRVAYLDSAVTSYGPTEYSVLLRGNPDHPAALAGAARGVASAEAPASAVLADDGSLSASVPAQPLQPPQQPQAPGAVPRALFSPRPPSKNDGICLKIATVEGEGSGKGKDGSSFFKSSDTFSTAVRRSAAAATVGSSGSNPTMELYRVRLPYNRYSKRGIILGEGKPENQNHASIFCFGEALQTIDMNQDNALAEALKMRNLLHELAPEPASRRLQAIASHPRGSTSSEHHRHAIASRTAREVPVALVGFREWIFSDVSGALGTFAAACELAFGTIVQRTMSYPGRVRLHYGHPDVFNKMHIMTRGGLSKATRQLHISEDVFGGFNQLLRGAQIKYKEYISCGKGRDMGFDSINAFEIKISGGGGECVVSRDVARLAPRMDLARLLHFYHSGPGYYINSLFIMTAVWLNIWVVAVFALARASTVQRLGSDGNLHLEDTLRVEHALSLGPLMLLPYAAQLLLEWGALRTFATLAVQIISGSVAFAVFRQQTTAYYFKDDITYGGARYISTGRGFSITSSSFTTLFTNYARSHLYPGMELLHLLLLYAFVRDCKGCSFAAVTWGTWLVAVALLFSPFWFNPMAFTREKVSRDWSAWLGWMRGEVDSATGNNWHSWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGS0</accession>
    <name>A0ABQ5SGS0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="354" mass="36761" checksum="0DE37E50B6D70696" modified="2025-10-08" version="1">MAASHPVLAPPAGLTSAASHVAHDRWVPTKESNDYLDHFDERRIKNQVPLPVPPVTSAYSNQDIDFSSPTNRAILACNGLPRNFKAGGRCVNPLEPRYVLPGCSPSPSAPGTAQPSPKAGTPASETPRSASPAARRPGERLLVSAMSLPRIGDGPGTLVSPRLQPLPHSPSGHNNTATAFGGQPQPQPSALASNPVLNVNGAVGGLLLYGPTAFASPAATAVAATIANRNLDVTDINGQRRSASVPPRRNGGFDPLSISDIVPPRKQSPTRPLACLTTNDIKGAGPRARTRDVPSPGHGTLNSADIPGASPACLARPSPTSYALGRWPAPAVPQYHFAHSTRVTLLKKPVTVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGY8</accession>
    <name>A0ABQ5SGY8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013272</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040848">
        <property type="entry name" value="AAA_lid_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012099">
        <property type="entry name" value="Midasin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17867">
        <property type="entry name" value="AAA_lid_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010340">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="7">
        <location>
            <begin position="4331"/>
            <end position="4523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2279"/>
            <end position="2313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2850"/>
            <end position="2880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2975"/>
            <end position="2999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3164"/>
            <end position="3185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3522"/>
            <end position="3550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3563"/>
            <end position="4189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="2286"/>
            <end position="2298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="3164"/>
            <end position="3184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="3522"/>
            <end position="3537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="3576"/>
            <end position="3592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="3599"/>
            <end position="3614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="3615"/>
            <end position="3624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="3640"/>
            <end position="3678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="3695"/>
            <end position="3707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="3711"/>
            <end position="3734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="3746"/>
            <end position="3769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="3777"/>
            <end position="3786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="3787"/>
            <end position="3805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="3850"/>
            <end position="3861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="3948"/>
            <end position="3970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="4029"/>
            <end position="4039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="4089"/>
            <end position="4102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="4109"/>
            <end position="4118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="4159"/>
            <end position="4178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="4523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="4523" mass="478554" checksum="B063FD68B4D508C9" modified="2025-10-08" version="1" fragment="single">[encoded data, 4523 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH64</accession>
    <name>A0ABQ5SH64_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013303</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="211" mass="20610" checksum="9AE18811A04B399E" modified="2025-10-08" version="1">MGCGASKSKAVHDPAAQHFNGVHPPTAAGGYPAPPGAYPADPAPGAYPPAAPPPAAPLTGHLRRSSSSGSSDSGRKKHPPAPVAYQPAPAPAYGATVQPGYGPPPPPGYVAPPPPGYGAPPSYGAPLPPGYGAPPPGYGAPYGAPPPMYGGGQPVYVTQHGSGYQPHQQQHHQGMSTGAAIAVGAVAGAAMLGGGLLLADAMDGDIMDVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHF9</accession>
    <name>A0ABQ5SHF9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013629</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13156:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13156">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-A SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="152" mass="16462" checksum="D7C1EFC7A9AA8645" modified="2025-10-08" version="1">MALQSTSVIANHFIGQKFFSAFATKAGSLATIDWKHVSVPEATLRDIAQTIGGIVPKGDLRATSGLGLGDGIKNHTDKWLQVGKSPMEYINEVEPIKVKGLVVASYGSDDPALGCPVEYITLKGTSYDNPAVCKYTGNRYYSDDWKHGAHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI57</accession>
    <name>A0ABQ5SI57_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Multicopper ferroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013941</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF486">
        <property type="entry name" value="MULTICOPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="341"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="534"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="757"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="877" mass="94829" checksum="700D5CE8D445A3DB" modified="2025-10-08" version="1" fragment="single">MISAPAKEKQPEMSMTVDSEPNSVFGSGTDDVKPGQTDVESPADVASTGKATPFPAAAATADADGGKRRRVLWIALAAFVVLAVGVGVGVGVGIGLKKKESNIESVLSSVELGGVPGRTYFIAADQVSWNYAPSGRNLCYNEEVAAKFLPTGTTRVGGTFVKALYREYTDSSFKTLKPRTTEWEHLGSTGPLIRASVGDVVRIVFKNNLPADISVNMAPHGGVVAWEGAGRKSAGTAPVAPGATTMYLWLVPPEAGPAPNATVSSRLWLYRSSVDPTTHDNAGLVGPIIVTAADGAGPDGLPNDVDRELVVIFQVVNERSSNMLPYLDSHLTGGVSFTKMAMNGFVWCNLPQSAISLNVGQRVRWHVASVGSSDGLHNYHWHGHTVELNGHHVDQFTAIPGATYSVNMIPDEPGTWMFHCHVNYHMDGGMVNLYTVQGKAAPLLSTGVERTFYVAAEEVEWSYTGPNNTNMCGDVPVAFSDNVTGHQFVDGPWAKGTDSRLGYKLTKTLYREYTDATFTTLKERGPEDAYLGLVGPMLRVVVGDTLKVVLLNRAAINVSMHPHGVRYSKASEGTLYEDGTTAAQKLDDVVQPGQTYTYIWQATERSGPGPRDPSSMLWMYHSHLDETAETYAGLSGAIIVTDPVRAVNATTDPRPIDVDRELVVLFTVTNELESSNFMENLRSRLGDGGALAAHEDAVESLIADAGFQEHMLKHGINGFMYCNMPRLNFTQGQKVRMHVLVLGTLEDMHTPNMGGPRFDYNSQHTDSIQISPGGMISADVVMNSPGEYELQCRVSDHVTSGMRAKYEVFPDLRVVEATTPTSVVRKYYIQAEPVDWDYAPGGYQCNGVVDAGVQSGSNTYLLKTSYTMGSKYRKAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIM9</accession>
    <name>A0ABQ5SIM9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014130</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF430">
        <property type="entry name" value="RECEPTOR HOMOLOGY REGION, TRANSMEMBRANE DOMAIN- AND RING DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046731565" description="RING-type domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1465"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="1666"/>
            <end position="1707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1575"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1638"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1883"/>
            <end position="1919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1931"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1650"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1961" mass="207482" checksum="0257DA721440D8F0" modified="2025-10-08" version="1" fragment="single">MWAGLACLCLCILPSLGHGFNWTTALNASNVAPEPRSLASLSGFANESVIYVYGGFSHSRNAWLTETWKLQVEERHPQWAKLAEGPFQHASLVNLDGVVLLVGGRNDDGRINDKLYWLNVTTGTWMAAKVKGDAAPPLYRHTAVSYTVDGLPRVLLCGGLKVTTNATVNDVYELSYDVASAEYMFEKLSNMPQPLFGHSLAVGANSKVYVSGGISGSFEVLPTAALPPKLAQLMDVTGEPSKSTASASARVFEFTRGLESGNWSELGTAPRPSAFHASWIDGDALYMHGGVQGRAENSSAAPHGLQIVNILNAAASPSFYNLTAVNEALGLWGDLQPGNVSGAAVWVNSSSTDPLQARVWMFGGWATPQPLPIWTDSYLSPQDSSLIWRLNSSVSRWLAPLQGGMAPRGAAGLLAVPMRVRGARKMLVLGGRAAKGWTGAMPGDTALSYALSDLPLSPLNDPLDSARAYQLYDMWLIDQVTGEREVVWNFACPAYPLGSLECPTAVGRLWDRRLLEFYDAEADPGGSAAAIYLQAEEMAGTNDPNSAKLQPGIWKLELDVINLSAADGPFNATVATWTSLLPKASTPVQPGVKSCYPRVLYGAAHLVSMIFTNTSYIALHGGLVTSNNSLWNTVSSSVCLLELGPVTRWLLQPNFAYAMPQARYRHACASRTGLKNYAMTCYGGWGNESGRVLEDAWTLTLPGGVSVGAGMTTFIGEWSVAHANYTALRPGPRIDHWMLPVRGVTKSNSSSSAAEAFSPVLLVGGSATAVTGRQAPLPHQPIWLLDSAACADPDAPLSNSTSAWKPDRRAQVWLSLLPRGTLPGVANTTAGAEPPLHTWALAAAAIVKTESCSELWLLLAEASEGAVGDVRAIKAVASSGGCLSASERPYCGTLLYSDDVAMRCWSGAELLLSTPGVTLSGSPDALKLQSSSGGFTLVDPDGATVPFKGVDIASGSFRSGIMLPPGLKLDDLTLVAYGRPYIYLGNTSAGQNWTLSLPPPYKVIVQLRLPNGTLPSSISIELRVRDWDGLDRKRRSDNQLALNTGPDIKASNVIASDLCTTGANGTAEFYVMNTSFMHFPGVVSYVVAFTLPDSVKQYTVQLKSPNEAATTTVVMVNGKNTNTLPNGGTDGVYITFNVTFESPNGTELQLPELKQRNWTAEVLSLPDKNCVYNFSSPYFNKTVKLPKSLPPGDYSIQMYDANCNTGPGSLYYLKASTDFVVQDNNMPTVSVVIPVSSVWLNVNFNGSVAPGILSFVKPTNVWVRLFTMKPTVALWSQSMLGRNDNLWALLPEMEYMAEFYFPCSEPCSSAQEINLHNYNFTTPSLLNGIGTYLPLNIILPLTLLCGRPQWLLSPVLNFIENKRSGMFLDSRHSVAYINGTNCTWRINTGQPVADFRIMYSGWNGTFTIRSDKLIDVLKDTTVNKLYQVLVSNVSIEFVSNTNGVMENFQVSWEGHGIRTSMPPQFIMMIAASSAAAGAMLLFLCLWHCVFRPVQMRRQQVAAAMAAAASAAVNAAGNAPGGRGGNRNRVPRRYLRMMTTSVYSVRDAAAAVTASAQAAAASVTSKDALKVCTSDGAANKDADTTSAVAELPGHHRHQGILDAARERRALERPTSARLDKPQPAPVAVVVTAGAVPTVGPMTSLPAPPSSSGPGSHSEPEASGGEICAICLSEFEDGEHVKHLPCKHFYHVACIDQWLGRDITCPLCKDNVLDALKSLFGPLPARGGNARQGRVGDEDAVAVGVGTAVAAAPAPAVPGGDDIRGLGGGAGRAVSGSGAANGGDLEMVVILPYDPAAPGGNGSGGRGILTELPGSVTTGDSGPGRHSVGHGLTAAVTNRAGSSISSGQAGVMASAAAAGAVAMAAGPAGPVSSSDSRELRPTRRNISADLTADQEGTSSDCDSGDDSEYDNVSHQPRHGSRVAAAVVAGAAAANNTARSHGFRSVRAPPPMPLGSGAEVAATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ42</accession>
    <name>A0ABQ5SJ42_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="144" mass="15848" checksum="E0E5C1480BC797AF" modified="2025-10-08" version="1">MLDHNTAMPVARGCTCATGAFSRMDLIRVCVSLRLLEEVGNEARFIHSARICPNPAEQGICGGQCDRGLKRNGWACQWQPHHPVPSARTARTQIPPESFRRSICELDKRCTLCWIKLFPEDGGSLLRHRTEALSSPTEGGSCGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ66</accession>
    <name>A0ABQ5SJ66_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Membrane-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014405</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="29958" checksum="F8EBEED3559AC5EE" modified="2025-10-08" version="1">MPAGVEPSPDEVIVVLAQVAFIALALAAIVFATRAAQVSFDNRKVEVPVRLQAVGSSRQRLRTLSDRSQFHRAAAATSAVGTDGAGVGAAVPPSIWQRGDSHDLSLARDPSSPYQNAAPNSAEASGGSLNVLTAAAAALLHRRHVPTSPSISTDPSSCSPASGLLSTTSTAPLSTASTVSNSSGATTSTWAWQDQTPDRSRSLNGHRQHYQHHYQQHGRGSGALQHKCSDSLLETCRVFASVPHETITSTRQVLAAFNYDYSALHRRETSYVAGTSAWHARTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJM6</accession>
    <name>A0ABQ5SJM6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="360"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="847"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="205"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="816"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="860"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="875"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="947"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="983"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1050"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1070"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1087"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1101"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1118"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1173"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1241"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1285"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1362"/>
            <end position="1372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1396" mass="144093" checksum="48704FB276545D86" modified="2025-10-08" version="1">MSEVSDLRHEVVREDVTGVPLLHIYLPKGLLSRDLDVSTDNSPDALQVFIEHIPTERVLSLIELPGYASNEDSLVKYSNKHRRFTMRLKTEPASPATAGSPASSHTVAAAVGVPGEQAASPEKPSPPTASYTKHSSVPSSSPASTAAPSLLPPAGTGNSFGLLDNDNIDDDDVDANGEGTAGAGKKKKKKKKGKKAGPSGEGQGEADAAGAPAANGNHIEGETSTRAAGGGNSGDDAESKPAAGEEGSTGAAKKKKKKKKKAGAASSAAVEEKGAIAGGVKAEVAGDEGPLPHRSSPPPPHTPSELPSAPNSVPASTAPSAPISKHAPRAMFVDMLEDFGDEKLGTFALGAALEHDPLLGPAVHSGLGKVQFKGEDKWLEAPTNSWCFKGLDGAYTPVSAFGIFDGHGGRMAAQHAAKQLLPLVAGFAERAVSPDAPISAEQLAAEGYLAAEGSSVVAGAAEDEAALGRGRVMAAQDALIARLPKALHAGFVQCDEDVISRHKTSGTTATLAVQVGWELLVANVGDSLAYLDTGSEIVVVSANHRVAESTEEQERILNAGGRIKPAKYDEDDLEGDMPGSATPHEGQQLRVWPGGINMTRTIGDEAAKGLLIAEPSVRQISLPVTGARLIIASDGLWDAVNAKTIIGQLRTCNAREAASKAAFYATRNKKHDDDVTVVVADFVPRQTDLHVPGLLKRASGPSAAALAALADAGMKEERAVQSWRPLESHAESWRLRHRVHRQRAAAYLHQLELAEKAEVEAAEELERQRRMAVAPAAGTAGVGTAVRSPPVSDTYRELAGLNISLDTLESMKDEPADGDLGDWTEVDGKKKKDPGFDFRKELIMAARPGEGKPGRLPSGSGAGAGGAGGTGAGSGREERRETSGRRERRDRGGGADRERRPPRRERDSGGGAVPDTARVLPAEGTAAEVDQQQQQADQQRQRGPRGQRRERGVDRRERGGRSGRGGRTGGDSAVPAGDEDFNSASTATAAPTAQSVPSGIVKADGYLYVPKSLLNSSGSALAGASVPPTVGPAGSGSAAVAPGNALEAASDAKPRRERGVRSRNRREGASSPSAAVQDSTTKDLHTAEPSAASAQEAPSSNTSRRNLQGGRQQERESAAAAVAAATAAVGGLSTSASAAARPAAHTPATSSTEAVTLDSSSGHRQRRQQHPYSASQQTAYTAAQATPAPQEPAAAASRNNRPAQHRPSGHAAVSGPTTVNPMRPADRHSTPVQPQPPPQAQPRSQSYQQQAQQQQAQQQQQAQQQQQAQQQQQQQPRAHHHAGLQTTQQQRTPAPAAPPAVPTYAGPLDFGAGFGPASGMWPPSGAPSQYGGGFSANGGASVPQRPPGLSGAPTQRSQSEVAAAAAAAAAAAVSRKVAASRGSGGGAPGQQPHGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJN6</accession>
    <name>A0ABQ5SJN6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-phosphatidylinositol-4-phosphate 5-kinase</fullName>
            <ecNumber evidence="1">2.7.1.68</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014884</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.68" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="5">
        <location>
            <begin position="321"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="126"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="584"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="599"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="623"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="911" mass="100930" checksum="C49E158AC350C2D7" modified="2025-10-08" version="1">MAGAEVGQPPGEHGGLAGQQPGIATLKYSDGSEYKGTLKHGTPHGIGTCVWTDGNRYDGEWRNGVMHGFGTYLWTSGQRYDGEWKDGKRDGVGVKLYADGSTFHGIWRDGKKHGVGVFKPAAKDKERDRAKMVVKSNSAKQMGSPTDAESVSNLRTFESVQPVDATPLQADSCSKESMHRGAVESAVATASPGVLAETDRDHERGREREGYRDRSREREKERDKEGKAVFIRAFDDGTLRREDKLSSEDAKIIFGQLKPQAPEEKRRASNARKKVKRALGLGKKLQQKHGQLLYKGMPGYNLMLRLQHGIRWSVGRGSYGRASSEQGPLQPPQHVNSLGEATGPMPLSHTLQPSDFTEKVKVFFPSAGSKETPAHPAGDFKWKDYSPRVFRNLRNMYGIADADYMLSLGGSSALWQLNSPGKSGCMFFLSDDERFLVKTMRKTEIRTLIQMLPEYYKYMEMQPQNLVIRFFGVHGVKQVHGRTVRFVVMHNMFNTDLQIHKKFDLKGSTDGRTTGPKVPPSDPKTIFKDLDLDLQFLLEPAAHAAVIRQIASDSEFLRKVDVMDYSLLLGIHFCNRVRAAQDNETSITHQHQPEQHPLQSEQQQRQTTSTVSPSEGTGGQNISQAGNGSSPASGAGAGGSQQLPVALQSVSSHESTDGEETAGGQPHSPGALGGLRLGSGDDYDGEHVRNFDLFSDRPELDKQLRLIDERMRGLGYSDQRVQDILSLVRLRILGDQMKMRSKKAYKVYDGPSAVLMALASRRHGLELGRLDEAEEQPGSDDDLEDGQPIGKQPPYLGYQMRALAVHTSGDQPPEEVVLCFGIIDILQEWNARKVLERTVKSFMHDSYAISVAPPKLYSSRFADFLIRTVFLDPDTAASHGLRSLPSVAGTFGMHTTRVHTIEEERDSDQRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJS2</accession>
    <name>A0ABQ5SJS2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Photosystem I reaction center subunit V, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">PSI-G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_014753</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PsaG/PsaK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.40">
        <property type="entry name" value="Chlorophyll a-b binding protein like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017494">
        <property type="entry name" value="PSI_PsaG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000549">
        <property type="entry name" value="PSI_PsaG/PsaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023618">
        <property type="entry name" value="PSI_PsaG/PsaK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016370">
        <property type="entry name" value="PSI_PsaG/PsaK_pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03051">
        <property type="entry name" value="PS_I_psaG_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34195:SF1">
        <property type="entry name" value="PHOTOSYSTEM I REACTION CENTER SUBUNIT V, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34195">
        <property type="entry name" value="PHOTOSYSTEM I REACTION CENTER SUBUNIT V, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01241">
        <property type="entry name" value="PSI_PSAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01026">
        <property type="entry name" value="PHOTOSYSTEM_I_PSAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="9" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI70019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="138" mass="14409" checksum="FC37EF108E5864E3" modified="2025-10-08" version="1" fragment="single">GKAESSPGRVSKTPSVKMALALANRPALRASAVARRPARRTVVTKAALDPALVISGSTAAFLAVGRFVFLPYQRREANFDASVGPKTTGSTYFDDLQKTSTVFGTNDPAGFNIIDVFGWGALGHAVGFAVLAINSLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK70</accession>
    <name>A0ABQ5SK70_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="168" mass="18762" checksum="2F5197979460B2AE" modified="2025-10-08" version="1">MMVLATSAARIHQKEKENKAARRRQAKSSQPSKARNSSRNATNSSKAAAKGYSPFILSSRLVTNDDDVRPSAKVRRTSTGIKRKVEDKSTDGDDNNDSYDHQRKKVKSASEDVRCTRARRQRGSSGSKNKKDLLEGEVSKDEQSEDEQDDATIAREDDDNDDSEIEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK83</accession>
    <name>A0ABQ5SK83_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040320">
        <property type="entry name" value="At4g37920-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31755:SF3">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31755">
        <property type="entry name" value="FOLATE RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="470" mass="52562" checksum="93D49D9EE4A1AE20" modified="2025-10-08" version="1">MRGLPQRNGMSRCASKRNVSAAAQRLARPSLLLARHVPVINDQYQTTSSSATGSSAVHAGSSAPTCGWNGEATSSERSWTLRPCPSYSEGSSRHRRHRGAVAAAAAAASSASSSSAPSTSSTTAGSSRVHSDDLVSPKREAQLTLEQYKEIYDRLISIFQTRPRDDWKKLIVFSKQWDVHAQGVFDRIKELADKEADVDKKMALRKLFRALQNVNDEVSRYNRVLLKLAEAADDEWDAIVAAQRGDLQKPFFEHMQCLLVAAKEDPSRLDQLVLINTRLVALIANHDAVEADQDKLDAAAEVYRDLLSSISSVEDLDRRMSELASAGKIDPAFLQITAKAYGAARDTNMSKDEAKWVAYKLYRTARDHFDRQQPAEKRIIEYLLNVVDPTERRNMLDQAVTPGPVRATETHDYLYSTPQRLFLVLDNTLRAYFAMRDGAAKNLNTPEAAVLPRKIRVMMELRDDLIRRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKG6</accession>
    <name>A0ABQ5SKG6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="510"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="724"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="842"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="929"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1048"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="613"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="947"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="972"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1059"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1114"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1173" mass="124660" checksum="DDF89B07420540CD" modified="2025-10-08" version="1" fragment="single">MYSQHRADYLTVSRPGQRSGVVNAAANHGFAAVQCAPGDGNAAPFRPFSQIANISQSGSRQQALAGSLHAPMPRAPSASQGSNNNSTQQPHQSQQQNRKQQHNMGGVLGFTSTSGASGLPQALVRPFSAGSRPMGHNASITAGARPISAPSQPMQLKSQLASRPMFASLSMRPALGSGGTGTGVTGNTGCAAGLSATAATVTAAVVNLNGYINMSQKSDDRDLYHAGFSASQGSAGMSYNDCVQFRAGAANRSRGAAWPSAQGSGGGGGGVSGGGDGGDGGVVPNIRISRTSSGATNGPRPTSANATQRHDQHRPYSAPNYYEPDVEPYDKSAALENYAAARESQQQHGSVLLTASQVEEPLLPFPLASGSAGSGLGVTGLAQEIQLRKRQDAGALATAAAVASKVPGDSGPQQMAIHQSENCTLPARLDAHHKQVISEPHVGQVMASCELLLNGQAVQESGSSAAAAVPLPSIAPPGTNTRCAAETAATSQECQGGYATADFRCGDGQGLAPGLSQQEPRLWSQREQRQDQAPQLHQQGRLLSRSDEQQLPLPLCDQEPDDDHQREERRRQQDQQRSNKLQNPNQQQTGQHCHRSRQLEERYHLSQVTEEVQQPTPAGATPQQLPSPPHPALRRLHQQLDSQELCQQQEQRLAVLVSSQEPHQHQQLQVLRRNLEESQSQQELTRGDATKESDCRVVTQMRKGSQPHQETEGQCLLVRDPAHQHRLQPQRRQEQKAPAETDAAPHGFGPSAHGAMLETCYSELHGTLNCIAARTAKVEEAMQVCQQIAASLPEVMSLLHHQNAVLQGLRTAQEDAAVAAATATAAMERRFAQALLRDEGQRRLGGGGGVDATAQTSPSFTLLPPDPGASGLRLTGSGAPEPRALFQGPPDGGGISGGPGVAVGAGMANSHGAVAGVGGVSVCAGIADGGNERPRCRRSVGTHRQLTTTTTTTTTTTVYEEEEEEQQPVGNNREEGDEEKKQGSEGQAVRSRLEGPCHTPASKGNGPGTARRVRSVIRVQQTHTQHHCSVLEHELFPEEYTGEALLCEGLRRPPPSPQSPLSEQQQQRCFQTTRDGDAQPAPKGGATGWGEPSLCRPRQPPQRHLQESCKQAHLLRQPPQQLPTETQPRQKERAKQQNQDPLPKKRHRTEEGRMAEIVRPPDIQAGAKSDAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKH6</accession>
    <name>A0ABQ5SKH6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014787</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="904"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1171" mass="125484" checksum="C5004C934C77B054" modified="2025-10-08" version="1" fragment="single">MPIVGNTTGAGMGCITSRIDVRDAVNGQAQKHRDDCYTPGTAADIASTSQPSKVALLTLPGLLSPIAPPTTAINPIAPHTTTYMPHKPTKRRLNLSSPLKPQRKSGNHDDGKASVKGHRTVQAPPEAVQIPTRTACGFCVKPDPVGLCLMESHGNVNYAAMAKATSHSSGIMVGNSPVAEEVSEEEAVVAITHPELNLVKDGMMAADGVGLVHARRPVRESVLPHGNWEGGGEHKGDEQWQSTLPNHQGKEGIKGERQRGHGSNTASAAVTSGGGCTDRPVRKAAAKARQRIMINLKAVKKVGQVLVPLRSHAENDVAPITIKAAASGSGGYGAGMVSRPGSSSVAAESSGWSPEVDIELHTQHTTLVDGTNKDAGGDSGAADAAGRRAMGVIPANVAIILGTDLDASGDPEGYLSATSKDDDEHGCGGAEVGPQADRSRGTHLSGTSAAATSGHSSGAHGARSDPEVQQQQQLQPELPGWDPRRPGYVELSEAVEYRQARQRALVKRPEDLPQCWGVGLLGSNLVVCNTVLRGSASLIFQEARKAALQGRLWKLESEALVQIVNDDERLTFEQAKALTHQFEERLRKAAAEHMTASVQPWQDGAARKDWRSLTHAEVLENLPELIRPSGKVEAVILGERPDDPHPWEDVPEMTGQASLRTRPGNNLAPGEFIGLYSAEAWLHPDWQAQILANPMDHWDDSEYPCPYRFSANRKHDFGSITCFPVRRGPVYFAPRTGYAADCSLPEKLLEHLDQLDLQDERLRTQLQEEPENCGKMFLTSARLGCMMSAANDPRRNLRAMCEAEAHFREGGPNAVVMTAVISGILPVLVMVATSHIAGGQHVMYSYGPAYWNLHEEEQSVCRDLETVWNELRKARAWKARRQQWRDLKCRLAALTHRWEETRVELQAERELRLAAEEVAANERQRRMEIEEELRALRAALQANWQPVGPEPQCGHEGRKPQPTPLCANVPPPCTSLGDLESQETFIMTDVAEPAVGGAMRSEADGGTESPEVVLHGSGECRGKMLLGSPLGKRARADTRNTIVDFEDSGMSPKTGGVTIPVNDGGLSGVDSSGGARRALAACKACGPSVTSTTTIVVLADAVDEAPTSARRYSRLSLRREGKAMLGEQGGALDATNAYICGAAAPARVMRSSQQQLMLDVGGSAPGIGAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKL2</accession>
    <name>A0ABQ5SKL2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="750"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1085"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="750"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="794"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="812"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1090"/>
            <end position="1099"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1109" mass="117264" checksum="A2586CACF7CA3B78" modified="2025-10-08" version="1">MQTYILQLCSALYMTHLPFFAPSQGIYVQVTTQWNMRDVKRASSDAQVDELPERRRWTADLRTPSGRASLEIRLPAPIFPATSAICTSAPICPGTQPGDKAARAAGMMPGAVGQPLRRAGSGAPDVVGAAPRLTSFLRSRRLAMPDPSVVAAGISPASLEAPTRQDLAGSNDIAGANTTGAGADNHHNARRSRRRPTVTPAVTAGTAITAFCTKATATRHTDVSFCSGENGSSAAIVVANVQDAAAVTSRGFAAAAKTVSLSARRAAFNQDVQPAMGKRERRGQTRQSIAEKTAAGPTAASASVDEVGPYGSLTDRVAKRTRREVPTGPLQSPTLPQPQQRHPMATRPSRVAAIKLPAKMPAGPALQLPNVFLQRKPPPMPPPMPQMSTKARVCQVLSDVMPMEVSTRQLTQKAVASDPPMLQLLKVPEPQLLSPSAQTKAGLLKETAQQRLLPSGLLLRASPPPAIMYEQPSMPKERAPAATLTLPAVTVVPSMASAAAAPQQGSWAAARLQVPVRSATDQATATATDRKLSAAPAEAAPLPLRVSTALLQDHLALSLETLMALTALSKALQVKEPVAPAPGGVRSPTEVPAAEEGQAAVLATQPQNLTSASELPPKSAVVTLMPSSCVDCGRKAGRTSYRAAEAPGHLACKSCYYRKMALDFDRKRERVIKTDASADAATGRYTATKSEGDWKFSPHTGVPASATTQKEPLLEQKQPIQEANAIPQSSFEPIRIVLWLYASQSAAPPLPLEPLPPRPAPAPASREQEQPTGGEEKDEKEEEEENVMKKCYEEIGDDGDSEEYEDDDDNKGSGGCYRRGGGGGSSLSREAKRQQHLRQQKRLQAHFEKLEKFRRKRRRRERRALKRLVLQPAPGELAALPPPSDFLTNFLLPQPMPLFLPEPTSKAGRRLAEAAKASMAAAAVTEAEAADSVAAIATDDTLTGVAATRVQRMLPQPNSAPCLVCCRIARSVGCVRQDGRYVCRTCCSSTMTWRVSLGADSTGGGGAGNASQAGRAAVASAGSASAATLPDGAGGSGEFSWVPVHRTWPALGAVQLDVEWTGDVVEEAVRLAELMMLVAAHEVGGDRSGSRCGAGSSRGLPGGGKRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKL3</accession>
    <name>A0ABQ5SKL3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enkurin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_015445</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027012">
        <property type="entry name" value="Enkurin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052102">
        <property type="entry name" value="Enkurin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490:SF0">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490">
        <property type="entry name" value="ENKURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13864">
        <property type="entry name" value="Enkurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51665">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enkurin" evidence="8">
        <location>
            <begin position="142"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="240" mass="27267" checksum="32C34E5021D755A8" modified="2025-10-08" version="1">MQEESVYALIPNPQEVPSRPPMHTSKFSGRTHPAQFEFGQSKVQPHATMGKADGNNAPVRLHPHEKEPKLPSPGPPTNPKQKIRPPIPSKDDVPKMGLTSNKNFITSNAVDVMLAKPGKVPQPEFQWTQKPDYGQVPLYLKRNKERMSKEKEQFSQFLRVREAPDANAHVSQLSPDDRQQLIRHLKNKWSSVNTAYQGLSLTVDTAMKKIRKEAMERELAEIERDIRTLERGEVVLVVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKM0</accession>
    <name>A0ABQ5SKM0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_014789</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="5"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="311"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI70166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="572" mass="63046" checksum="B8AAF8A66D3FE637" modified="2025-10-08" version="1">MQATKYVLVTGGVVSGLGKGVTASSIGVLLKNCGYRVTSIKIDPYLNVDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLDITLTRDSNITTGKIYQAVIERERRGDYLGKTVQVVPHVTDAIQDWIQRVAAQPVDGKAGVPHVCVIELGGTVGDIESAPFVEALRQFCIRVGDNICNVHVSLVPVIGVVGEQKTKPTQHSVQVLRSLGITPTFLACRSSYPLEDSVRSKLALFCNVPTANVLSLHDVSNIWRVPLIMQEQGAHRLLCAKLGLTEADKLDLTSWKVNLADRWDSLTDPIKITLIGKYTNLSDAYLSVIKSLQHACMEARVKLELELVEAASLEPESKESDLTVYDASWRKLVEADGILVPGGFGSRGVEGKILAANYARINKKPYLGICLGMQIAVIEFVRNVLGMTEANSTEFAPATPHPAVVFMPEISMTHKGGTMRLGARRTVLQTMSCISAKLYQQDQYIDERHRHRYEVNPDLVPKLEEAGMHFVGRDETGERMEILELVNQPADHPYYVAAQFHPEFKSRPGKPSPLFLGFILAASKRLDSYLRSRDFPRSATQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKN5</accession>
    <name>A0ABQ5SKN5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_014816</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orai family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.140">
        <property type="entry name" value="Calcium release-activated calcium channel protein Orai"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012446">
        <property type="entry name" value="CRAC_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038350">
        <property type="entry name" value="Orai_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31501">
        <property type="entry name" value="CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31501:SF7">
        <property type="entry name" value="CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07856">
        <property type="entry name" value="Orai-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="312"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="345" mass="38716" checksum="22360B4EA7132674" modified="2025-10-08" version="1">MESVLNTAQTILTGYDVTLQRQIRSEERAWRVDDLTHRQHDREVASQELEFMLEERTFLAAQIQQAHVDNARALWQRFVNRNRAEVQEKAEQLKALSALAALLAGFALVSFLEFQFVVNTDYNRALLPLLALTTSVTVGVEVAAVVLCSLMLASIMRVGKMYVSEQEEAEFMHQCHVFVSQYRPGHRPPAPLRTFAAYWAHRCEGEWSTAFRLFTIGVPCFFVNLSLAGWIKFHQASTSTAASLVTTCMGIALVWNLYTHYKWGKYLLQDEAAEQDAGLRLTQPPVGLPFDWYLPQVGVLDVPLQRSLTANPAQGLSSAGRSQAPTATHRTEENDVPCSDEVAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKV3</accession>
    <name>A0ABQ5SKV3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="1">
        <location>
            <begin position="215"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="508" mass="55397" checksum="A6898F7DAF0893CB" modified="2025-10-08" version="1">MMITQQQFLGRSSKRFTAITSAAAAMPTALLLAVAMLVSMSSMAAPLPSGAPPRLTCFYMHPRATPAAGTTFKGEVKGCQWATHNMLKSAVLSQIAAVGAVMDERVTPFSNCTEDSAVKSTTNFCWAFNSSAEGAKLQEWAVNLVTKLRLEAIGFETKYKCSPWLIDNRMDVIFGGYGVYPDIPPGDINADANKDAPPEMSNIECTPDLSRLNYPKSTCNTTMRNTQFGVQSWVYTYPGRKPTTTLYCFAVEVLNATDPTSPCGRTDTLFKAEIWGDDKQRQKLKGIAIQPVGAKNFTYRSPSWGAPGDQTIKVSQLNWTPQQANGSKICLELDSTTDIDSFCLFGHKTCWINFFDESLACCPLYPSTTVDKLSEDLASAGDFTEEDAKLLVQMIDVEKAFAKLDSQAKAQSTFMATASKCFDLNLWVFRLVGCVDTSKLAVSLKAYVKIPLLGSKLMGSCTVSLSSGPGCLLGYPKVASIRLYFKGRSLMMEASFLGKSTSFTVFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLF5</accession>
    <name>A0ABQ5SLF5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SNF2 super family</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015105</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="296"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="538"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="549"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="600" mass="65205" checksum="449B3163189173BD" modified="2025-10-08" version="1" fragment="single">MGLGKTVQVIGYLAGLQYSGVYRPSLVVCPATVIRQWLRELRAWWPPFRVVVLHDSAKHPPHAVGSRPPKPALLDVALNSPAGLVLTTYDNLRLQRDLLLRVRWGVVVLDEGHKIRNPDSEITLVCKQLHTVHRLIMSGSPIQNRLAELWSLFDFIFPGKLGTLPVFHAQFAVPIQVGGYANATSLQVTTAYKCAVVLRDLTAPYLLRRRKADVAAQLPAKTEQVLFCTLVSEQLELYRAYLASKEVGEILEGSRRALCGIDILRKICNHPDLLERLTAQGADDYGNSARSGKLRVAERVLSAWHSADHKALLFCQTQQMLDILEKLVRGRGWSYHRMDGGTPVAVRPRLIDDFNTNPDVFVFLLTTKVGGLGVNLTGATRVMLYDPDWNPSTDIQARERAWRIGQSQPVTIYRLITAGTIEEKVYHRQIYKSFLTNKVLRDPRQKRFFTARDISELFTLGPEYKKGAAAAAAATGGLGGLDGNGGGVGDTETARIFGATMDMQEALRRRGAAAEGPTSGRLSSTAAAAAALQLRGEHDGDIDGADPDNGGGGNGGGEAGSVGKAGQSHISQDNGAREPEHGTGRAGGGGRDGRRKRAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM23</accession>
    <name>A0ABQ5SM23_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015621</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="686"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="195"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="367"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="764" mass="83019" checksum="60B05DDCF7BFB2BC" modified="2025-10-08" version="1">MSESVVSDFGSEEAAAQTVAILTQFKTENVELKRNFENLKVLHLQLNESYKSLQARFAALFEERNNVEKQYQSLCESWRVELEEKQRHLEAAKAQILGPRDLDVLKAKLLEELEASYRAKCENLSREAESSHQAYIKIRRDYEELQNAYRSLEVRTVGEQEAARLEHTALSRDLRDKSAQLMGTQAKLATSESSVRSLQRDLEAAKYSASQMKQELEEVRRAKERAVVEREAAAVQAEKKVKAAEEETAQLMSFVESVGRKNRHLVQELAESQRAAEDLFAANVRLQSAQTQLQSQLDSSVRLASVEKAALIVEHEEAMRKLEERLATAQGESTRKDGLLAEMKLAHQDELTKMLVTWESRLAEEKRAGAERARELLDQKADVAERAAAQQRTTEERLREAEAAMRQAQSQADTAAREAAAEALRTEGLAKQMAEATALAEGLREELGETKAELLRSQMLVQQMSERRAELEQRLKVAEENVVQIRAARDALAAEAESVRREADNERAAVTRAAEAARSAWALEKAALAKRYQAAVKELGVRHEAELRKVKRKARGAQAAVAQLTDEVADLKFKAAEEQHASQMSEVLYLNPVTSGGGARSGSPYRDSLQLYSPLALPLNSGGGGLHGTSGGGRLPSPVRPITAPSYMNVAGAPSPSVVIVTGNGTAAGGVAAAAAAGAAAQAQQTQRRARVNDPQSDAAAGLEDDGEAHVATVVPVSAAGNSGGGGVVGAGGPIVDAALLSSIAALRHRQQQYMDAARLGVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM67</accession>
    <name>A0ABQ5SM67_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015999</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17872">
        <property type="entry name" value="GPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030228">
        <property type="entry name" value="Gpn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI71082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="282" mass="31377" checksum="561267FE816EF7C8" modified="2025-10-08" version="1">MGKYAQLVIGPAGCGKSTYCNHLHEHCQAIKRTVHVVNLDPAAEAFQYPVSLDIRDLVSLEDVMQELGLGPNGGLLYCMEYLEDNLHEWLGEELEGYGEDDYLVFDCPGQIELYNHLSVFRSFVNFLKNDGWSICVVYCLDAHFVTDVSKFLAGALQALAAMVKLELPHVNVLTKIDLMEDKRHLDDFLFPDPRQLLPQLNASTGPRFHQLNEAMGALLDEFSLVSFLPLDITDEDSIADILGQIDMATQYGEDMEPRMRDEMEAGDGDVEDGGSDDGGNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM73</accession>
    <name>A0ABQ5SM73_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015750</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="270" mass="29460" checksum="2CE387C1256E10D9" modified="2025-10-08" version="1">MMLLNRSAQAAFSGKAARPAKVNRARLVCRAEEKSIAKVDRSKDQLYVGASQSSLAYLDGSLPGDFGFDPLGLLDPVNSGGFIEPKWLQYSEVIHGRWAMLGAAGCIAPEILGAAGLIPEKTNIVWYESGVIPPAGSYDSYWADPYTIFFVEIVAMQFAELRRLQDFRYPGSMGKQYFLGLEKIFEGSGDPAYPGGPFFNLFNLGKTEAAMKELKLKEIKNGRLAMLAMLGYGAQATLTQEGPFQNLLDHLADPVSNNILTNFGKAFGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMA2</accession>
    <name>A0ABQ5SMA2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RAVE complex protein Rav1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="56305" checksum="343AFFA8384D4D61" modified="2025-10-08" version="1">MPLSATHGGQRPFLPATRSHPSRCQIIPPRMMRHLRPAATGGFHPDSVGASPAADATASTDHLANPASSAPSSRIVLLYVISLFGHLRGHASSLAQHLSRTQHAAILPHLGLGRGAHACLPGTEGNTEAGDRSDTFLALNLLQNASHLQSRHLDDALQPLSRQHASQNQHHNAGQSALDGRLWISFILSYVPWSSRAIPQGTDSTGHALGRATAPLSSAPSPSQLGQGEAHHILTVHTSGLEFLWLSAASLRQRAVITALWHMGQAVEAADKARRRFPSPEEMPGLVHLGAVAAAARAEETVALALARTATAASAAAAGVPAAAAGASSAPHLPLLHHARRRLLAAATLTLERLRGLQQGCTTHVLLRFLLYSALSPRLRQDLHVRLDVLARRAVQVALLQEQHDAWLARQPATQPPSPSPFQATNTLGSATVAGSNGASVVAHGSSIQEPFPSFGLPSAPPYLSARWYRRVFAPEMLACARQLELEAPGSPPDDQTAGDPPGRFDLRDLYRLIRVVEVAVEDLEHYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMM7</accession>
    <name>A0ABQ5SMM7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="229" mass="24232" checksum="17E645BD1104DA5C" modified="2025-10-08" version="1">MASSSDEPHGPLDTLAEDLGRAVLDAAAAGNLSAPALLARTTETGFRLDQLATLIAQTTEIRTQLGKYLLSLLNLLKSTVVNTNMDNTAGTRVLPLPQVQGHDAIPEDPLGEAVALDKEIGATRPAPTAATQRSNRQRKARGGASSGVHGSGGAGPSSDPAKRRKRGGVPSSAAAQRSDPNVKRTRTDDSFMPKWNCNHSEFERRKAANVCLRSTKDCPLLPPFVPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN48</accession>
    <name>A0ABQ5SN48_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015823</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007263">
        <property type="entry name" value="DCC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044691">
        <property type="entry name" value="DCC1_Trx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34290:SF2">
        <property type="entry name" value="OS04G0668800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34290">
        <property type="entry name" value="SI:CH73-390P7.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04134">
        <property type="entry name" value="DCC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="115"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="180"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="396" mass="43258" checksum="2D0BCFCA9C5354A7" modified="2025-10-08" version="1">MAFASIHLTGRVVHQAASGRIKPVPVHTRRQAVVSSGTSAPCFSGREEPVHVGAWSARRTTHRSRRVLLRVSASEPLSVPVTNLTKHQTHRNQPSAHDEVYGEMSCPMGGVEAIMRKASQGIFTTTPDANLQEIMPLLNKVTGLPVLDADGSVVGVISRKDIIRVRKSGGLMTEKVQKHMTSPALTIPLRATVQEAADLMLKYGIRRLPVVDDNGHPLGLVSRSDIFKPLGDYNKVMEEELAMLAGKGSWQIKYLFDGSCPICSSMKELLERQDKGAGRIIFVDVSSSKYRPSKNMGISQDEAMTTIHAIRPDGSVLMGTDAIRSMYTVVGLGWLAFLMELPLFTNAVDLLYEFISRNRISLGGAMDTLLAGKKLQMSKAGITTCSDVDEECSVDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN87</accession>
    <name>A0ABQ5SN87_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="248" mass="27983" checksum="FD9D188D39738944" modified="2025-10-08" version="1">MQHSCRSTYYDARRRFSGPCSSSYTTPRCSLWQTARPRRMVTKASTSSIFDIMDRQFADIERQMGAMQRQMDRDIDIALSRAREAERQADADFQRAMTGASTTSRQPPFFQEMRPAPYVDIQRQEEVSPGAYRYYERIQITGTSGGSVRGYVAQPPPVPKAAAVPVSPALNPVFVGALVVLGGFVTLTAAFNRNYSLTNYAESKRWLLLALWPLLFLFSAKFREQFAAAIRGERVPLKREGQVASDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNN8</accession>
    <name>A0ABQ5SNN8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase III subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_016357</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the RNA polymerase III (Pol III) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the eukaryotic RPC7 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024661">
        <property type="entry name" value="RNA_pol_III_Rpc31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15367">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15367:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11705">
        <property type="entry name" value="RNA_pol_3_Rpc31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000777">
        <property type="entry name" value="RNA_polIII_C31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="86"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="160"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="209"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI71236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="261" mass="28085" checksum="9D2C3D5BB920D8F7" modified="2025-10-08" version="1">MSGRGGGRGGFGRGGGRGGFGGRGAPIGPVARDEDGTVLATAPAGPPPLFPEVELPEHPEITSRDKMLLLRRFELTHRSKTSPYFLELPQAHQDTGTLEEGFDPYRAPQPGKDGSAGGGHGSGTKRARPPLSSVMTLLAEYFPEELYSSKDMRASRLQTLRGQDAYFRAQSRPDDPSGLRRLEHLAKLESLGEEGGEGGPGTRKTLGGEGDEEEAAAEEELRDTDEEEDMEDDDYYQGEHFDDDEGYGDALDDGGDEGPVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP32</accession>
    <name>A0ABQ5SP32_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_017118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000120">
        <property type="protein sequence ID" value="GLI71777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="142" mass="15113" checksum="DF33002A969963E4" modified="2025-10-08" version="1" fragment="single">HQHKTCRLHVLARTPHTQAACKVSASRISTDPQGLFRDMCRKLQEAQQAGQVPRDLSGSVMQAQQFLARAAAPSMTVTIEQAQLQRIEDTLQSVLAMQQLPSSAVSEVAAPVQPAALAWRPPAPPVPLAAPSIAPSHVTATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ68</accession>
    <name>A0ABQ5SQ68_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016362</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAP" evidence="2">
        <location>
            <begin position="746"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="622"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="466"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="641"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="874" mass="93624" checksum="BAD46A9388FA5674" modified="2025-10-08" version="1" fragment="single">EDEVEEEQVVRGSSGQRLADSDVDVGADNHIGGVDAGMRVTRKSASGADGGPLTSWGSRSVRSALWSYAALRCHPGNDFIWQVCRHLVSERHLRSLSVRDLIQCVWALATFRQLERADAEGGLTAVREGKHRSQCCTAALAAMEREVVVRLKTGRAGLVDETQEGDRTQPLRRRETLAEAEVEAKAEVATRFGNSSGSSNGSSRHLQARMEGPEGGEGQGKPSSRLHRELLRPQHVADLLWSYATLEHPAPDLFTTLLPLLEHQLAFFSNAAMSRCTWAAAALGVYDSRFMDAVADNICSHRLPYLKPQELASSAWAYGELGHAHKTLFDGLVSYTVSYVWPRADRSMTLREISTVCAAAGVFGWRYPVLTDAAARVLARLLIPMGQWGHLRDKHGTAFARDSHSRCQPASAIPPTGLASIGLCSTTATAGASGSDEASAAAAAMAVTQHCTRSVSVHYENNRPASSAVSSTSGSSSSNNNNSTGDGNGTTPAPATTTARTTTGGLGPGAALLLLPSEHDVAALSWGFSLVGGCSPEMWAQLMELMGRVVEEEGDEGEKEKAGGARGGGGKGLSDEALARMCQAYLHMRLDYPTAALSTGPMGLLRRGAEVLRRQQQQQNRCACGADGRESSGGRNLPGAVQSTLSSAPSNALPMPTVAATMMQSPSPSSSSSSVSCVSAVSEALSRLGLSHTVQSLTEDGLFRIDLAVEVEVRVDTRGRSVRPIRPTTTENTAPTSPSTTQIIRIAVEVDGPRHFTANTRQPLSPTLYRRRCLEDRGWVVVSVPYWRWNQCRTATSDGATSSGPAEQEEALLLQLMREEGLGEVIEAVKAQPPPPPARQRRRRIQKALKVTASGKQQLQLKPDDESCVMCHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQA3</accession>
    <name>A0ABQ5SQA3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="134" mass="13938" checksum="B759AFE04CADF0D3" modified="2025-10-08" version="1" fragment="single">MASLFALAGGRNHVNTYSGLPNPRPIAVAMASGSQAWQAVPAVGALPDAVDQHTGRLDAVANSGNQTTLNRIPSMGIANEPVNVFAAAPPVPAMQMPSRRTCDRQPILPIAEQSLSTTHSAAELLRDVDGDDAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQH9</accession>
    <name>A0ABQ5SQH9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="2">5.4.99.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_016918</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767:SF2">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE TRUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="TRNA-PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase II N-terminal" evidence="5">
        <location>
            <begin position="171"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI71644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="456" mass="49037" checksum="E886D165F9341FDF" modified="2025-10-08" version="1">MVRLIKSASSSGAMAAFTSTRPVQSSSAAFIISPQSVPQQYLFPLPPPITLHGIPAISCRAAQAPNPKSAYRRPQPRPIAAAYDVPQSCHSRQLVATSLPHYRHRCNTFTIAVAAAQTPPASTSTASPLEPPTIMTSFPAHLHHNVLSNGLLLIDKPPHWEVAEVVTAIQRATRADKVASVAPLDAAASGLMLLCFGAATRMSSRVEKAAKRYDGAMLLGSAAASADVRGDSYVSEALPWSDITDEELQEAADGMLAALGAGGSGSGSGSDGTVIQILPPRYRLRQYPSSFKYYEEDKGSSLSTISLNLHDFKVWRMREEDICRTTTSAAAAGLTARQKPTLRTTAAISPASRASPTDFRDPWVSGADDAVSVSNRDLPVRGFDDRPSGDVTGRVVRFSMLLSGRAHVRSVVAMYGRRLRTCACLDDMRRTEVGMFSVADAWPLEALLPIMERYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM01</accession>
    <name>A0ABQ5RM01_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000001">
        <property type="protein sequence ID" value="GLI58384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF8">
        <property type="entry name" value="SD21168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="99" mass="11345" checksum="0CD57B134412124C" modified="2025-10-08" version="1" fragment="single">PFYLQVAPRAPHHSMHYDFTASGAFVSRHWYPPVPAARHWEELSGAALPRPPSFNDPWVSGKPAWLRALDPLTAANVTFLEEIHRLRLRCMRAVDEMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMQ3</accession>
    <name>A0ABQ5RMQ3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000002">
        <property type="protein sequence ID" value="GLI58386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="142" mass="16323" checksum="08B758FBCE188FFB" modified="2025-10-08" version="1" fragment="single">MLHSSRMVSCWRSCGSSHGSRMRKCGSSTQQQDEKMQDFTRQQDEKMRKFMVQQDEKMQDFARQLLDEKMRQFMQQAEVIRENQVARSYNVFAIDADHTLEPLLVESVQAQHALGTAPSAVPVNFPATVDAMSKLTNHDLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMS1</accession>
    <name>A0ABQ5RMS1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Component of cytosolic 80S ribosome and 60S large subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000102</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="5">
        <location>
            <begin position="8"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="4">
        <location>
            <begin position="84"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI58401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="27606" checksum="489BBF3D6B14B81D" modified="2025-10-08" version="1" fragment="single">TGTMTTAVPELLAKKRKRDEQAAAARAAAALQSRKKSRATRKEIFKRAEKFVTEYRAQEKDLIRLKRAARAKGGFYVPPEAKVIFVVRIRGLNKIAPKTKKILQLLRLKQLNMGVFLKVSKPVLNMLQRVEPYVAFGYPNLKSVRELIYKRGYAKIKGNRIPLTDNKIIEEHLGKHGLICMEDLIHEIFTCGPKFKAVSTFLWPFKLSSPKGGMDKKRLHYVEGGQAGNREDKINAFIRKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPY5</accession>
    <name>A0ABQ5RPY5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="23496" checksum="F030A0FDA9DB71FC" modified="2025-10-08" version="1" fragment="single">DSLIALNFKASNCVHFIYRKQTSQLQAVLPVPNLEADMGKSRGGKYHSKGKGPRKGAAEEDGSDEEEYEHQQRGLGGQRANVGMLPPSDSEDEGEEPSSKGAASSSQPDGQNKNAGKMPSSSSEDDDSDSDDDPTPEYLRTAPTARKKEPVAEPRSEEQVRKDLERLELIRKKREEDRQKRIMDEGWDRFAPISDTNRPPGHVPSDHPSKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ86</accession>
    <name>A0ABQ5RQ86_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="100" mass="10592" checksum="B10232A690ECD3A2" modified="2025-10-08" version="1" fragment="single">MAPAAAATTCAAVAGTSSRPPGPPHTGHEDEVIKIAIAVPKLNIPMDVKLSDLGRVAAPLTRAFVRNVRNYFATARGGQQDSTRRLFLATAFEGAARLWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ94</accession>
    <name>A0ABQ5RQ94_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001697</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046418693" evidence="3">
        <location>
            <begin position="31"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="534"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="624" mass="64770" checksum="DE1911D37C486D8D" modified="2025-10-08" version="1">MFIHNGNVSTWRLWNGVGWFVLLIQAFALAHPAKGPVNLEAAHVVSGSLPAENCQSGPPQTCTNGKTAATEASDVGLDSAVGSIKWDVYQVDMIRASGASTRLAAAADDGMFVIHGGASTDTTAVSSVTTAAATDEGVTRRRLLTSSTISNGSASATFLDDAFATWCASDATCAAVGSTPKLSLAYYTNTSPFTSITLNWPNIFSGAGVSSLQLISGVVEIQLANYTTKQDQVICDNVTSCTAQLTIPLNSSADLTQSFVCLRLEPSVTGNSYQAYGYPPGSYGTSLESTAVNGAMRCNTTRFGKHVIMQYKQSAVMPPSPPSPPSPPPSPPLSTVGETYTSDVALKMTVLLSLGFDKLAANATLLSGLTSSLESSLVSSITSASNSMLLLPATSNISRGIRKISRASNQSTEAVSVEFRLLVPAGATTGQVQALMQLLSTNTSGVFSDSPYISYFTAAANVSYISSSSSSGLSTRAIIAIAVCCAVATLLLIAAVYGIYRWRTYTAILPKKPDGPWDVTGVAATLARTGRRRKVQQAQLDATPDAAVGLPPSPPLPTPPPTQLPTQQGGPAAEGHLQNDFPWEGAAAGGVAGVPSAVVLTDGGDQQRVKSKAKLTPPIRDAWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQK1</accession>
    <name>A0ABQ5RQK1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cystathionine gamma-synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001516</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044639">
        <property type="entry name" value="CGS1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43379">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43379:SF1">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="51196" checksum="087CC63FDC62365F" modified="2025-10-08" version="1">MFGARGLCHVSAADVPAAVSLRAPIPAVLRSAARSGLHVRKPTFHRRRPSSFVCCTAAKDLEATSVSGNGTSMHHEVPTPKLSTAAVHGGERAGRPRVADTLTTPIVQTSTYFFRNTAELIDYNEGRFDSFEYGRYGNPTAMACEAKIKALEGADDCLVSASGMNAVTSMLLSLVPSGGHIITTSDCYWRTRQFMQNFLPKMNVGVSVIKPNDLEALQLALEQHNVTLFFSESPTNPYLRCVDIAAISRLCHAKGAAVCIDSTFATPINQQALALGADLVLHSATKYLAGHNDVLAGALCGKPELVGKVREFHHIMGGVVDPHAAYLLLRGLKTLDLRVERHNKSTLEIAKRLEQHPKIDRVWYPGLESHPDHAIAKRQMSGFGGVVSFEVRGGLQECISFIDNVKLPYIAPSLGGVESLIEMPAVQSYWGFGPEGRAKIGIKENLVRFSIGIEDVNDIWNDLVQALEYVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQK7</accession>
    <name>A0ABQ5RQK7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1</fullName>
            <ecNumber evidence="1">2.1.1.57</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cap1 2'O-ribose methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001838</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that mediates RNA cap1 2'-O-ribose methylation to the 5'-cap structure of RNAs. Methylates the ribose of the first nucleotide of a m(7)GpppG-capped mRNA to produce m(7)GpppNmp (cap1).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67020"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="Rhea" id="RHEA-COMP:17168"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:167609"/>
            <dbReference type="EC" id="2.1.1.57"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.57" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050851">
        <property type="entry name" value="mRNA_Cap_2O-Ribose_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16121:SF0">
        <property type="entry name" value="CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16121">
        <property type="entry name" value="CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="3">
        <location>
            <begin position="124"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="553"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="574" mass="62141" checksum="40194C2942DE52C2" modified="2025-10-08" version="1" fragment="single">MYPGPMVPVPLVPMTMIGQMPNGHMPMPAPGAMPGAMPVTADPGFEQMSRGARGGGNADGRGGGGGSGRVANSAAVFQAMEATRKKTAAGQETRHEMRERMRHIMFEIDQSNSDFIGGRKEPFQFLDVCCCPGGFSTYALTAGSAQRKGIGFSLPPALGGHLPAIPLETDKYLLHFVDVTTVAAGVRVGDMAVGVPGCPVHALHLNAAPDAPPVGTCHLVILDGSFLGGKDWIHKETTLPDLDNPAFNVYGSETAAHKALLIAQLIIMANNLGPGGVLVLRLNMFTDMFTAGVLGLLRHVFHGDVCSYKPRTCHVHRGSYYLVCTGFNPKRAYDMQWASRWNACLAGLRRGGTAPRFVPFPGVHLDSQSTLEVVWTRALYVYHVHLWRFSKLVEVAMEDKAILDHWMADRPRKGRFTHFCGRIFAGVGCRMSARCHAAHSFEELHPFVLKAFHEPRGFLFMPGSIALMQSPLPSFDPLPETLLELKQQAHMQAILQAQAAAARQQHAMAAAAHAASGNVFGYIGSYDTAVEMGMGGEWVHGMVDCYDQYGNKNNNTQAMAPPSTSPVDDTQLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQX3</accession>
    <name>A0ABQ5RQX3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002025</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF8">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="397"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="614"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="765"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="631"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="647"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="811" mass="81944" checksum="74205B740BA554F1" modified="2025-10-08" version="1">MSVLTDRMLRDAAQEDDLSQVVALELRNRGLTELGPIDRCQKLKVLDLAFNQLKNVKGLEGLDKLKELRLSDNRLTSTSALQRLSDLHTLALDGNLLNQLEGLLGLRSLKLLNVSFNQLSSLSSLGRLTSLEVLHVAGNQLTDLEGLTSCPALRIVNACSNRLQHLKGLARCAALQELHVADNKLTDLSGLKSVASTLEILDVSGNGLTSLSGLCSLPKLSELYARENALEGLDGLAARLPALELLDIASNRLAMSAVQLAQALAPLTDLAALQLMGNTRLCSGVAVNDTAASSSTTGTTVTASGTSSGIGNYAMELFAALPSLELCDRQERVQVLVVSEEQAGAAAAAGGNGTVLNSASAAQTEEEAGPPPNLDPTIAEMEAFRKRLGIRTYAPQYAGRPATAARPGTAGSRPGTSAGLRPGTAGGGRPGSKGRPPMSPATRLAGGVVMDPDAFAAALGDFRTTMASYSTSLKSVLTKMRANLKLPAYEAAQRLKAEGATQLPQMPLAPEYGTRPRNTNLALLGGEDREDVEKREEALQMFERLFGGSNGDAAVGEAAGSTSASGSPKTSGAKAVHAAAALATAAANGGGVGQRPDSDDNEYDEYDVELRSSYTAGTPAPSGRGGGGGAAAASTSRPGTGVSLVGTGRGGGGSSGGSTLRVAMSVKPPAAVASTPGGFVCHKPALLTKKTVVPGAVGSGSPGSPLTSPSSAMPAAITAAATGKTTLGTEKLALGSPRLVASPRREGGSPGVSAVTTAATAAAPTAGATGVGGCSSSRLGRPTSAGLAAPRKLASSLVPRTGGKMLAGGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR11</accession>
    <name>A0ABQ5RR11_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="45567" checksum="4670A8FAE3069C8C" modified="2025-10-08" version="1">MAFLQRLAESFKEGLVTLTNGGETPRRNEAPSRSLAKQSSKGKSRPSSASYGFLESHIRDAIAAFVQEKFDKGEAKPHVFTQFWLQFPKLEAGFEVLRQQLEARTGSREGPLPLGVLQSNQQFFGINESSLVVPELCNKAVSNKCTDIDFIGLVMLLLVVHLTELSTPGGGSLLHPDVRTMLSCLELAFTHFNSARKGKLDKREVAEALQYESAKAHMKEGARGRTSNRLAHRLFEGLDWEHDNTISFENFLRGILQLVQDEFGDMPSGDEEEEEELAAAMAPSAANRSGSRSGSVTPPQPGDADGGAPASRGTVPRRCGSSRASSSCQREGSYSHSRPRSNSATGLTSGGSQSTATVTPGAAFSPGPGQGPESVGRPHNGFLATLKGSFRKSADILMLKGGDAGAAAMATPRKAAEGSVEGTAAADM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS70</accession>
    <name>A0ABQ5RS70_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="526"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="675"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="834"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="891"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="458"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="537"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="715"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="891"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="945"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="980"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1007" mass="106293" checksum="CB8CDD553B9135DD" modified="2025-10-08" version="1" fragment="single">ACMPYWRAAGPSVTMASAGHAVSGVEEALRARANARRIKEHYEGTLGVPSELQSLSHLLTHDKAAVFAGLEKLPFYSKSDVPLPPAPSGSGPLAPDPRHPPHTFQVPQPPHRRQHPPPVAMPSFSSQLPVFLPRGSAAVSLPPGPSLASPGLWPQDPATPAASHTGATVDGADPLELHHLQQQQQQQLHPEALQGDATEVPPAEQPYPLEPGIGPRSGSRVLLPSGFTLDPWRWTPGDDPLNQTSDVSPGLLDLVPSDNHLQLQLHHSHTYNPSHPHGFGQQHPPPLRRGSTPAGTGTGTGTGARPTAYQHPDSRIVTPGSASSSRNGSPTRGTASGAPGGAFGGFRSPSPYGTGYGSGSGAGSPTGTVFGSLPEAETEEERKYRLARQRLLSPNDLTPREWARIGPPQDAADLEKFSARGYSVSVDFDRQTLLPKRVARPDIFHISPEEAQRRQIEADEATLALQAQRRAEEEAQQAAAELERQARNAARGPRYDALGVLQTEGSDALVLPRFLDWYPGSVDAPGQLMSHSQSGSPGTAGAPMTAAAPPRGSRCRSAMRSRSESAAVAVYGTYGRGAGATAAAAAARRKSATEQNLRMGEGLQGPDQYVYEDYGDDTADNDLGVGAAYGTRPPRRRHHSAIAGSFTLPPRPAGVSDAHYAQMVRQQRRWLNAPAQPQRPLSAVPFPVPASQNYSRPRSSRPWPDGTAAPVPSRGSAQAATAESANRPTSALVTVIHPRGKQLPPRPGQRGSGGGHSHKSISRNVSTKSGGEHLATASFINAVSAAFPAAKPQQRETYAPQNSGISGISGIGGIRGINGNASAVPLSRPNSAMAAPPGEVVQPPTSEPLASGASSAVQLTYSAPPDGKRIPWEFREHVAAAAAVGSIPQQPALDAGDASASSLSSLLSPPPPPLPDVAAVRRRRSTGHSTASAGGDRGSQRHSQQHQQHQQQQQQQQQQQQRPGRWTGSVFGETLPPESSGRDGEQYQPRKFELRTDGMVLDGYLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSE6</accession>
    <name>A0ABQ5RSE6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002628</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF30">
        <property type="entry name" value="PHOSPHORYLASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1399"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="573"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="729"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="790"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="858"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="963"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1043"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1085"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1289"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1421"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="734"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="758"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="800"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="889"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="979"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="997"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1043"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1089"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1289"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1312"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1353"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1435"/>
            <end position="1444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1824" mass="195543" checksum="398E0E5F55B3AE98" modified="2025-10-08" version="1">MKALCSLWLCLGGKGEGKAVELNVTPSGKDKDVRLVADGNVEVKRSDGGKQRVSAKSYLEDRDTAAWRVAASEIVKNCEAGAGKSPVVTEKTTLQERQSFLAGDLHNERTLRAILQKAKVRLSEQTQAFALEPQANGPFADSGRAGAASDPDLVRLNAQLLETCSVPRPDSRALLRLEHLLGEVRCWLAARPCPAAATLVLYTVGEGLVHAEHAIRSVGLVRYWQPENTSVFLALLQLYSDLLIQAARAAQLQRQQQDKEQQRLDKEDQKPLPSQQQQHQLQQQPREKGEQQNQNQNQQPSSGSDVVVLAADKEHLLLHHQRQNQRDRLVTEKLLPQLSEMSLEYSEQGPSQPHRNLPTDHQQVPLTFAAAALLLRPDWLDMLAEVLELLHFSEEYRLAVARAGKEPQEALAKALQLWCFLTQLVKFAAAHAHSVQRMEPCLQRLNALLAPPSGAVPRLLDLPLAEDTLVAQLHALWLLQVLYDMPDAKLLYSAPLAEYYIALHHNAMVQSYEASAADPKSAAAELCRIHATLLRSMARHPGATVRSAFARIRTVEWILQELSLESQLLPAAQQDGVADDPDTDSDSANDSDITVSDDDEGEDRNDAGYSDKLANAVRETQPRSAKMRPLSPVVSTMDSNTAAEAEVEVEARMEDGATGPGVDSQKASKGFSFTYDLNEDVERLIALEDELGREMELDGFEYDEEGKQLKARSDARLAEARAAEAAVAAAAVEQPTTPAGQRMQQQQPTSAGAGDLQSYGSLASTSRRSGGIPRLALSSLRTASISSMAAYSPVGKRSSRQGARTSHTGNGTLFVREEEEGQEPSSDDARTPSPNAATAAERVAVWSSSLMDAYAAASAGDDSKLPHPPPRSRSRGRGSFGVVGPPDYQTPSCVDSEQSQQQRPPRTTSGEDRAAVTTSAMTGTPLAPADEPTVARPREITNGSPVHAGRQGELHLLVQSSGTVRVTQPSGTPAGHGRPPLPAKPALPPLKMGSMESAAATAAAAPPSTVIPSSPSVSARAPQPSSSRAMMVLSASNRVNMLASSPTATQQQQQQGQLGPSPWAHTATAPMSSSPVCELTRQCHAGNDSSAPAVSQQQSQPASMGVLIPTLNLKSLRRRDTTITPPTDTGRTVPLSSMLRFTNGGSLSNCSGRDSANTLLRSGGTATTPASSSATYHHSHAASNLGMTSGMLHRITETEQRPPEVAYQEIACQRLLYQDRATHILMLKVLIDLLVAPWGTLDPAYVPQYPAHCGAPHAEFILLWHLNAPRNASVVHDLCRSAQRRAQQAATSAVRRTAGVVTTTAPRAPQPRNGSPQGSTHQQQPTDSARDYSPSSSIVSFQPKIQQQPTVQAPAPRTAAGASSPGAGAAAVVAAPTRAGAVTRALQLLSRPLFDTSRYTNLQFVARGTFGDIYRARMEPPRTQAWPSPTSLGEPSASPQAGAAPQPPQPQPMQVVIKTITLPSSPHDSCVLADVFGEVSVMERFKGHECICQLLDYGMRDDAYWIVMRRYRCSLAEWRGRQKPLSDLRAATGGAAAVAAAARLYLSALTQVVDALRLLASHHVVHFDLKCSNVLIEPLPGVRDGELWAPVGSDSVGTATANDDNNDDNAHRAPFRCVLADFGEARAYRCAEEAFTARNRGTEVFKSPEMLMLNAASRTSMKPASATTPPLPTPLWTDAAVAAASDSPFPGESTSPSAAARGCGATIARPRTADAQRQGLAGAGLASDIWSLGCLAYELLSGTVLFGGDYASVTHRVAFGSGEQLTLTDSERARLGNLPELVGLVEWILARNPAMRPSLDQIGERIELIRSALGTAQVLDAASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSU8</accession>
    <name>A0ABQ5RSU8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002391</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="317" mass="34336" checksum="56DE0EF9BCC6CE15" modified="2025-10-08" version="1">MGHLCTPVEGHRHVYPKILHRDIKPDNILVTKEGVLKLCDFGFARYTSCGPRYVQHGTQYITTRWYRSPEALMGDIHGPSSDIWSVGCTTAEIATGKPLFPGNSTADQIWRIVRCLGKVPTHQAALVASNRTLHSLAITSTQGQSMLRHHLPADLDPHLFRLVESCLQLDLMQRPTAQEVLQWPYFGGVGRCLSEAAQRYLTAAAGGSDRRCGNTGGGFKMLPCTHVVTVAPPSPCHNHQQASDDATIVAAPVAAPVMPLCCGRAAVIDDKLQVPADSQTSSATSVPPGATASQPWLWWMLCRLVPSSSSLSTTVHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSV5</accession>
    <name>A0ABQ5RSV5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="10742" checksum="B908CE96C625028D" modified="2025-10-08" version="1">MALAATVSRKALAVRNPALTHSVAPTFVVSSRRVSCQSQLLSTVAAAGDVDAPISVVLGAAVVVSLVASAIVPLALSPGKKAADKIFAATEKAPLDKKPVAGKKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTY1</accession>
    <name>A0ABQ5RTY1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Malonyl-CoA decarboxylase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003331</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.150">
        <property type="entry name" value="Malonyl-CoA decarboxylase, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.90">
        <property type="entry name" value="Malonyl-CoA decarboxylase, oligemerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038917">
        <property type="entry name" value="Malonyl_CoA_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007956">
        <property type="entry name" value="Malonyl_CoA_deC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042303">
        <property type="entry name" value="Malonyl_CoA_deC_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035372">
        <property type="entry name" value="MCD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038351">
        <property type="entry name" value="MCD_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28641">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28641:SF1">
        <property type="entry name" value="MALONYL-COA DECARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05292">
        <property type="entry name" value="MCD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17408">
        <property type="entry name" value="MCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malonyl-CoA decarboxylase N-terminal" evidence="3">
        <location>
            <begin position="110"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Malonyl-CoA decarboxylase C-terminal" evidence="2">
        <location>
            <begin position="187"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Malonyl-CoA decarboxylase C-terminal" evidence="2">
        <location>
            <begin position="530"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="391"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="630" mass="68017" checksum="8CC602BEBD64579C" modified="2025-10-08" version="1">MQAYRELQNPQERLAFLSLVSQEMGMSVAEVRSAVRLWDAIMIRPSLPPAVAAPAGALASRAPAAAGQETAPTTSVPNSPLAHVSGGSSSRGGGDGGFGGIEFSSDVLFRAADRISNACRPLYTQLFLPISQAPQGMKCLVDLRADLLHLTTDQQQLAHPVPPEMKAQLRAMAEHLRQSLAEWFSVGLLHLQPISWEESSAALLERVMQCEAVHPLSSWEDLRRRLGPRRRVFAFTHPCMPGEPLVVLHTALMSKPADAMSDILDEQGVSKQVRQQQQQQRQEQEQRPRHQAQPLTKASHPDMQELPSTAVFYSISSSQPGLRGIDLGQFLIKRVAERLIAEHPSIRQLVTLSPLPNFRSWLISRLRQEQVMAAASTPPRPPSCLPSQVQAGAGDADRTSSSSSSSTGGEGGDTPLLHVKEAEAVVRLAGAMGWTTGAAHKAKGRDAGSASCVAHTGVTGAVDADTDPRMDAAAAAAAAAALCWLLEGDRWLFHGGGGHLPSEAFGDAPGDTRLAAGGGGGGNGGGSDEVCDETSAERVLRPLLMRLAARYLALETRRRFALCPVAHFHLRNGALMWRLNWRADTSSLGLSRSFGIMVNYEYDLSSMYDNNWKYIMLHEVQAHPQVLELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUJ1</accession>
    <name>A0ABQ5RUJ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase M41 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003589</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="61"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14105" checksum="78C33A5A8974637D" modified="2025-10-08" version="1" fragment="single">VRSFVDMAYKRTVALVEKHREQIEAMTAELLRKEVLNLDDVERLLGKRPFMCQELRNIDRFRHGSGASKAQELVTGEKTMETETGPGEEEGGAGGDGSGGAKENSDDTSGDEKRVEGKKGRRPGLVVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVA3</accession>
    <name>A0ABQ5RVA3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LMBR1-like membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_003724</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31652">
        <property type="entry name" value="LIMR FAMILY PROTEIN DDB_G0283707-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31652:SF0">
        <property type="entry name" value="LIMR FAMILY PROTEIN DDB_G0283707-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="439"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="486"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="60179" checksum="16EB276380B1775F" modified="2025-10-08" version="1">MRGRALLGLPQGFNWFLILVTVIVSLLVLGACIYILVEYQHPEDRNQAWFPKIIVVFSMTLAIWTVLMFPLDVGNTNACATNISPSACTYTLPMKQLWYAVFITNLVLVFVIIPFTLFFYEGDSDYTFCQKVKGSLMWTAGFLIFIILIIVIMYMLIGYVVYPTQQLTSGVRSLRVLKNINTYNVSCIPPATTNTTTNSTITADEQCSAFGNKFVLKNWKLRVSLPVYIMAIQSVLGWLLFLVFAGVGLLAAPIDWLQEFLGRPRAIITKSEYMRRAQIIAQRAKQLMSMLQLLRRGEHDRRWRSNFQKVQREVALLEDDEYQLERVFPQGEDGEVRWVMFMLGFWMLAFMSFLGFCLTGVWVAHMIAYMLPPVPLNPFLNSMFVVLDGVFPLFGTGAFALFCLYLMAVAMKGNFMLGLNFLIIKLYPMRPGSTMMSSFLVNTALVLVMAPAIVQFCAQAFAVYANGTSIFDVFGNQVMYLIGLKYIYNFNIFLYGMLAVMGLSAVFLAFRGKKVWKRRNPEDAYSTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVP2</accession>
    <name>A0ABQ5RVP2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="287" mass="31912" checksum="1B85D7C49280351A" modified="2025-10-08" version="1" fragment="single">ELFSMISIIGWPKQMGLRGGIATRQPDAVFLPTRCVRRRGGFTCSLWSSRDNSERERKDPASWRSIEDLRLYLERPQGPPGFISFLLTWISQNAFGAGTKPGGLAALYRSLLRMTPTPAARCSARHRSDRVDVTTEVLVRAQGSPLARILLISPDVLAVMASKVPSLLAMSADEAVGRLTGLKMLLPSCDISHLVATEPRLYLEAPREEVEEQLRCSLLLLHKFSLPHSFVEAMITQDPALPWVLTETGLVELRKLWPPDLVDVQVLLDSDPRELALALRAVSTRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVQ6</accession>
    <name>A0ABQ5RVQ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004173</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025224">
        <property type="entry name" value="CCAR1/CCAR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025954">
        <property type="entry name" value="DBC1/CARP1_inactive_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045353">
        <property type="entry name" value="LAIKA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14304">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14304:SF11">
        <property type="entry name" value="SAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14443">
        <property type="entry name" value="DBC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19256">
        <property type="entry name" value="LAIKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01122">
        <property type="entry name" value="DBC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="1758"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="216"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="314"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="386"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="498"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="629"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1028"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1182"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1362"/>
            <end position="1754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1833"/>
            <end position="1874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="325"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="386"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="430"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="565"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="632"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="650"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="662"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="683"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="763"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1035"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1182"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1225"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1396"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1458"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1524"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1545"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1573"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1642"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1687"/>
            <end position="1697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1698"/>
            <end position="1709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1725"/>
            <end position="1751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1862"/>
            <end position="1874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1874" mass="192300" checksum="7013EB332542C59A" modified="2025-10-08" version="1">MDGYNQYGSSYGQGRPQSYGQNTQQPHNQSGTGGQQAAGQQANSSYGQSQPNYSAGYGSGGASGGYGGAGSGYQPQHGSYGSATGTGGSAGIYAASGGASTYGSGPGQYGAAGANAPQYGTSVGGPKYTGSSGAPTAYGSGTGSAPSSYQAQTNYAANPAGSYSTGGSGSTYAPGATTGNYSSTSGGSGFSGSAAAYGSGGAGPYGTVGSNANTGAAAGTRPTATGSYGANTGPSPGAGAGTSSTPAGSSAGATYGAYGAPNTGASQPVPGLYGASSAMGTAARPGGAAAQALAHMPADQQNKLVSMVQQQNAYGQTGSSGAGVQPSYGQQQLTQQPPAQPQPQPVQQQQYGAYGQGSGAGANTGAASGAQQASVGAYGTSAPRTYGTGPTTASGATAQAGGTYGAQQPSQASAYGSGAGGTKPPAAGYGSSSKQYGNTQSQQSRYGGMPQQSTYGTTTAQPSTYGQPTGQQSAYSAATGPQAAVTVPGLGGAQPTGTYGAGESYGSGAGSSQQVSGARQGGYGAASQQQPPVTGQGGGVPSHSAPPGLHGGSGSTSAYGAPRQSVPSTGQGQYATNTYNPSSAYGGASKLPAPVAALTTAQPQQPAYGSTYAAAKPPVVGGSYGGGGMGSSAGAASPPSRGAGPSSMGAAGGSGGGPGPMGPSVGPSSSAGGPGPARYYSQGPGGPGAMGAMGGPTGQRMGPMGPMGGGPPVPIPMGRPGPYGGPPPLREDDRGRGALGPMGRRDYDDRGPSSMRSGPPPSREFRDRDRDFRDRDRDFRDRERDMRDREPRDREFRDRDARDARDGRDRERSDRDRERMAPPQSRDRDRDRDRRPSPPAPSRRDRDESIRRPQPRSPSPVPAAAVRAPPPRRRSPSPQPAGAVVRPKVPDYGVRVPQHCLTTLERDYLELVRRHSHLAVPSDFSRLVCSWVRAQPGPTDSLGISLLPLDRHIRYDHEQDFTRGPAVVPGGLGEGGHLTHGSTKYVAKVLLVQGLPPSLRQDVLLGPHHEGGAHMVNALKFVAARFSRPQSSAGGDEREGKDKDGGGKDKQRGVLGALGGAWDLELDGGDPDSEDCGLIRTCIRHVKDQAGVDLSQCAHWLRVCDVTYVRALPSPAAANICDLGVVIGGTQASVGMLEPSIREVVVLFAAVPDACVVGPDAWPEEARQQQAFKQQRLTLEMEAKKKEDTGSARREKDSASDKEKTKDEKRDKRDEDASFPAVAEAAKDKEVIDSPKDKDSKDKDKEADKGDNKAEPDATKDKEVREVPLPDEPGIQLRGRAGAGAVLGGGERVKTFSISLHGLLDYDETDRDEPTFELSVFAECFQDILAREYGGVVYAALVAERDQLMCPPAAALAAAGATVTSADAGSQAQSGKRVREESDGMECGRAGSEGLELEKGIKKSRTEDDTAVADAGQLGTNNVDDVGPTGQVTEAMSEGQGPADAAGADKPAEATPSEARDTTMAEVVAERTDLSASETVKLTACDVTPAKVEEASNEEDTAEESTPGKVSKAKGKGRTGSTRGRGRGRGARNSKGRKGAKQAEEAPEEEPEPEQEAAAADEGDPKAGCQAGEKAEEVLDVKLELKPEAEPKAEPEAIADAEVSAVPEIKTDVASGTGDGDVNTHAKEDPAVEAQAADGGVQEESTVEAVAESVEPAGDELAPEAMSLKTEPVQEADMEIDNQEALTPPPPPPPPPASVAAAAAAPSPAKDAREGSTKSQWARSSSREAKDTQDRDRERDKEREREKEKERKQRKKYVTDNNLLLAFRFFDRTGCGYIRTDDLRRLLHSLGFGLPHRVIKELVRGGWDEVAANCRFKGDKLYYRDLTDKEVKGEHGGASSLEGSEPPSMPLTLAAVGGADANAHREASEGALEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWB9</accession>
    <name>A0ABQ5RWB9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="103" mass="11523" checksum="8ED8E994BF05C3AE" modified="2025-10-08" version="1">MLDEEAKELEAYQSSRSHIEYLKNKGAEDKAMGCFLVGCKEHQKKRPTMVGAGLLLHMAVSWPVLGILAVSECKDRIQWAKKKWGLTDPKLTAPYTLMGTLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWL5</accession>
    <name>A0ABQ5RWL5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004579</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="276"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1354"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1454"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="366"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="500"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="681"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="879"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1051"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1284"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1389"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1354"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1455"/>
            <end position="1478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1498" mass="159830" checksum="E85A44B6F8EDF846" modified="2025-10-08" version="1">MWGQLNNLTKLVSEQAGSVIKEAGLDATLNSAKQQLTGQLDKLASSVLVIEPGQPVKAQAGGVSGLGDDYTTNVANGQESASLSQLRDENAKLQLQVRKLQETAADVLGELEEAQDALAREQAARATAEQRLEAAQKELERQIQGAASQSAISEEVQKLKEALAASETNAKAFEMKGRRQYSQLITTKQQLTELEASHLRMQELHAALEQRHRELQEELEALRNLERELHANQRGNDGAGGESPGVALSSSQVEELRSQLQRRNEQLHQLQIDLEQQRQQDASARESQAEAEGKARAQAEEAKAEAARLRTQLDSALADLQEAELRSQQATTESTRLDTELQAQSLLKEQLVAEADAASAVLSGQLAAARQEAEALAAEASQLRAELKQQQQALQEREVTLGLERRRAADSEFGRADAEDRAAAAGAELERCKQRLAAEEARYRREMDELNDSLDEMRRQADEHGRAAASGAGLSQRLAVYVGQLESAAALCEATTLTPYTSYDQLLTELQERLAAVQDQGGKVGGTASPATAVLPPHPAQEALQQELSCLRSELAQVRHNAEAVTHLRKQLAARDSELYDNRQALQKVKQERASLEERLQRLQGQLREAREARDACGTESEEQRRRLQADLDAAGQRARELEAQLAAASVATTAAEHGAAAAVQRAADMEAHAAAAAAAAESTATAVEALTARAEAAEAQVRQLSGEVDSAAASLAAAGADAEARHSQLLQEKSRLQQELAHAVSELQAAQARMDALERERADMDAEVSRATAALEEAHMRAGLLDGERLKSEEVCSALMSRLAAMEAEAVELRRQLGECGEASKTAASALAGARSEAAQLKARILELEQQRLNGCANKLAWTAGERTACEGTRAQALQASERALAQAKSELQDTRSELAGARGQLASAEEKIERLSREVTNKASALEAVRTGSQAEVALAAQRLSIATSQVVELETALALATTQHDQATAALQHKLTAMEKEAVSARAATRQLEQQVSVLRDSLTAERDAAAATAALVRSELEAKTAAEVSAATTALSKQWQARLDDATARHTAALQETQQELETVRQRAQIAEEELAILQAASGDMERLRKKVADFGPLTARLEADKAAACKAAAEAQAAQQQLEVRVRAMETEAAERNRKFQMLQRSFKGEVESARQQLEAVQAQIASHVTAAATAEQRAEEAMARRQAAEVERLELQQQLEDARVAVTRLEASLSAQLAVAVEAAGKLAAVEAMLAEERAGQAAAQSDGDSLRQDFERLVAAAVAAREEEAERRVAAAVAREQSALAEARQAEARAEAAELAKIELTLALARVDDDGRSAAAAGPSGLRPSVSSLGHVANLVGTSSEEYFTGSSNHGTAANGDATSTAEVAPSPRGSAHDRRLAARQAEILAEMQQRVDEAELAAAVQTRRAVGLEAEVATLQQQLSECQRQVKELSWQICVAVGDTPVTGGGRGPRGGGGAGSGGAASGSGGAASRMGMFDILGCGANYRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXE5</accession>
    <name>A0ABQ5RXE5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004150</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF74">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="101"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="290"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="432"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="547"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="812"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="583"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="610"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="633"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="815"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="841" mass="88535" checksum="90444EB9F866A32B" modified="2025-10-08" version="1">MGNILSPEAGPLFDAIAEGNVDAAVKALEAHPRLSHARAGKKNRTLYHACASSGQVKVLKRLCEHVWQTIPDELGKIHSESTPEGRFHPAIYRAMNTYDETGLTPLMLACRKGHADAVLHLLSQGADPWLGDRLLARSALHFAARANQPACIEAILNSPFVELTGRVRSKECKLVDYPSSSGYTPLHYAAGSRAADAASALFRHGASPNAKTFDMGFDFIQLDRGSTPLHAAARFQNLDLAMQLLKHWDENLRHLDVTDPRVVDNHERIKPYQMPGVKMNKALARVLDPDTPIREISDPFGETSRPNSVPVVKVTPSRSATAPLSHSQVMAGPLLASRESDISSPGSGRGSGGKTPAGWEGPNTAGGERFTVYQCAEFEANCDSAAQVPAVPPHADAAIKWVSTGLGLHRSQSDGRRVVLGQGASVNGLSRTRSGAAIPAPSDRFSSYRRNSFGDSDGERGQDCRTCPAVGAPAVVATATMDKSAAGPVAPNGVMSLPPYKSPFMFGTPAVTASGAGRGPVSECQLPSPGGRGQLLTHTRGDLAAAGGLTKTRSREGGADAAAEVTGARGGLACPANDGPQRSAEPSSQASSNSTAGGNPVSRSSSQKLSGGTHTTTSSTIPAADPAVPALKPSLSRTLSSSRRGSSPTSRDVDAPPPVFSAWSDTTSTTSTTPLVVAGITVSSRKTVMKDKPAAVPDFSRDSHSEGRQPPVEVTMYPHRLVSVEPLKAGEAGESGDARTVTMKAGATGTTHRITHHDRKVPHITHHHQHHHQQRQCKGGTDFGLHAVNQSLGSLSLSLSLSRRGLMPTVELSEDESRMEDRGAAVKALSPGASRRSRAMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXH7</accession>
    <name>A0ABQ5RXH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_004167</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06127">
        <property type="entry name" value="DEDDh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047112">
        <property type="entry name" value="RecG/Mfd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47964">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47964:SF1">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046259706" description="DNA helicase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="2038"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="1566"/>
            <end position="1738"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="1769"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="198"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="619"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="799"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="943"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1173"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1367"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1459"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="628"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="958"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="971"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="988"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1183"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1384"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1472"/>
            <end position="1482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI61747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2038" mass="215062" checksum="FEFAFDF568FD91DC" modified="2025-10-08" version="1">MPRVVGLFGGCILLHFLDALLPPPANKRLSRSLLVPPLSRPTPPAPLQKLPWNPPPGRQQLRCRLGPTLLQTSSKVFFAAVRAITAAAAAGGNQSIFADARGSLLHSTTAACRSPCHRAMIFRPTPRGPPPPLPGWMLDAPPAPGPMKPPLFPSALMRRVLVSWTPSGKQMGVSAISNSAGDAADVATAASELQYRPITLPPRPAAPPPPPPQQQQQAPLHSVLMTALPNPSPPAPLSPSPGQPPPPVVSPPLPPSSPARFSVFSLPGMDLKGPPGYQASGTELLAAVAAATPDQHAVWAAVWELMPLLLHVTADGPLRGFSVDVETTGFNWRAERVMELAAVDLTTGDTFSSLVNPNGAGCLVKLDPRVVAVTGITSEMLRPAPSEPWVTLAFLCWLYQRCRAGGGVRPALVGHNLRTFDCHFLGRMLARQGCLVPYSWCSTDTLSLTRKWRLWPGGGNLKLQSLREMYGIPQREEHRALPDALLTVDILAAMIRHRVTGSPGPYPPSSSAPREVVQQALNDGTLTWFEGPEALAGHGVGGAASLAAAANSLPRGRRLGPRDTSAPVRGDVQQLFPWNKLRELLVHLGAGTAAAAEAAATPYGSTGGQNASATGASEMYTRTSPTDGGSGVSGGAGESAASGDGVGDVVTRRQHNKGRWTMADVLLSKTATAKMRASMIVPCFHGPDFNSAPQRWCVRHPSNDSYSLPEAGEELPEDVPPAPVRGPLDMALEAPQYHVDDTSLLPKRSQAVNPAQANSGSTARPLVPAPLLKPPMPSITPAAAAGAVVTPTKAGIGAREEEDGEPFSGGSLPPLPYSSGGGIPEGAHEPASGSTSTAVPWISLRREVYGESQPAQLSQDTRGCSGGDDNDAIIVEGHRGVTFAAATGVSLAAVTTQMQEAVAGGRGDGTVAAAAVEMAETVAPEAAALMTISTPTSVAAVVRGRRSRLKPPAQSATPRPPPPPPPPLPSPSSSLLLSPSPAVLPGKPSTSPVAEAANLTSGSDVAAVTAQPPTASRRAAVKRTKPSAAADGAAASGDIVHGWEALGAPFSHEPFRTDLWLGLLPRRPPLLEKLEAAAAAGRLPTPHALLLAVPRAIVQHHTAISVTELTAALAVKDAMRSRLGSWREVRDELRETPGMPVALRAEFGNIKIMKLMPRGWSMTAELKLLPGQLLPGDSEAEGSGQGGMSGGTGSRKQQQQQQQQVLANLQVEATWSDFANPRADKAMRQRREAYLAGGSEWVVRGILRPKPAGGARVELANPDVLPAASFQRSWDLVTATYRAATPLQPGEVAAAAGALLDAIGNRKIVLSDPLPPAARRELNDLLTTGGHCSIMEHRLSSHGLTEAGSGAGCINSSANSINLSSLNKGSSSGVDIRPNVKSTSGSPPLRTSWSSSSSSWPPVSTDLPHISVPSLVESYEILHRPRLEHLQPCRVIAVSSADLAAPQHPVSTTEAVEAATAANESPLWRAPRAREGGSDPRDNVTGGEAATSPIPQTAFQVAGRVVAFHELFFSQLQMQQLRRSVRGPNWAPVSADALMEEAVASLGFRLTGAQQRAVSEVLADMRPDRTSTMYRLLQGDVGSGKTAVAFLAMLAAASAGLQALLVVPTTVLAQQHHRSLLDLMRRVPLSVRRRWGLEGEPLLLTGEAKAREKRDASQGLEGGSIRLAVGTHGLLNVTAFRSLGLLVLDESHKFGVAQMERLSHLIESRPPHLLNMSATPIPRTLAAAMYGHMDVSRLDEMPPGRTPVITKLVQDDTDIPYQDTDAVGEMEAMWEEVLREVTTGDTPGRAFVVYPLREASTAAAAAEELKNAADQAAVLAARFEPQGVSTRLLHGKMKAEEKAAALEEFRSGKVRVLVSTTVVEVGVDVPEATVMVVEHAERFGLAQLHQLRGRVGRGGRGGRCFLCVPFGDRASRERLGIMETTSDGFLVAEWDLEQRGVGHLFGSGTRQHGRIDVSGVTAAVIEESGVPAAGMELVEAARRAAVVVAREWEMDAKRRRPTIQVEEDREEASWCKVTLAAMAVFRSRDVTAAEALMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXQ6</accession>
    <name>A0ABQ5RXQ6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053209">
        <property type="entry name" value="Gramillin-biosynth_MTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47643">
        <property type="entry name" value="TPR DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G12710)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47643:SF2">
        <property type="entry name" value="TPR DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G12710)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="744" mass="79857" checksum="B4644C045C73527D" modified="2025-10-08" version="1">MLRVMPGSGHSVCPSVRDRILPSFRDGASTSGRVRSATSSLLPNRQRRCRDCWQCPAAKGGKGGSSQGFKGFGEPPKAPTKLDREMSFDPEDDQGQAAATVQVSQSDEEEIALLPCYQMYTDTGYKAQRFVAPLQLDQPVGEPLPVLCTTQNMLPGELALVLPALSMAEGGFQQVPELEDLHAALLEGGPSPAQRRVLAILESLRPGRPTSPAFVAAAAAPASGSAHPPTPASQQLSLPNLATFDIKFWSGRGKDSSAPAIDSRRLMKLLSRTAIADDSQDPAAMQARHQKPVGYVGLWPEAALVGHSCVPNTSQLVVADRMFVHLTEELTPGAQLTRNLIGAAITAPLSVRQAAVAEALMEQGVVVPVAESGPTPESRLQACHCQRCQLEASVSEGLRDTLAEAHAWYVNEASEAWNRANESEDIALLRGLLEECETIVAEVEEAVQSEPGLDDEQQDWLRSSAYDVYDMLVTLDELVNQDAANPDILRTCLELIRVHSPGSGSHMMVALKHEALRRHRLEVFSEMLKRDRGGLRKAISKGDKRKLIALKDAADVATEFRIEAAVLRYGLVTEAILAQLTEGLETYVEGLEQMSVMQAQGMTELTREMEVQGVKVQIVDRLEVSEAARAGPGAQVVSTTDGVQLMVVDAAAARRQQEVIEETTDVTEVQAWEAAGLGGLGLEDVEEEGGEGEVIDLEVDPDLEFDVDAAVQAAMDAALAADVADCESEEEVAADGSSVEAVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYD8</accession>
    <name>A0ABQ5RYD8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">hydroxymethylbilane synthase</fullName>
            <ecNumber evidence="2">2.5.1.61</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_005292</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMBS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00494">
        <property type="entry name" value="PBP2_HMBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="5">
        <location>
            <begin position="43"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="362" mass="38741" checksum="37A7196B756A637C" modified="2025-10-08" version="1">MMSQCYRSTTPCCHLRVFYAPRRCRVTRVCCASSPQAPPRRPLVIATRGSRLAQEQTRQVQQLLLAAAQLSDESLALSTLAIDTAGDTSPSASLRSLGSGAFTQALDQAVLEGRADMAVHSLKDCPATLADGLVLAACLPRADPRDVLVANEAKSLGDLVPGSRVGTSSTRRAAQIRHAFPYLQVVQLRGNVEARLERIKSGEISATVLSLAGLQRLGCEHVASQVLSVDEMVPAACQGAVGVICREDDPWVIRLLSSISHRSTFLEVSAERACLSALLGSEEAGQAGQPFPDVAWGCNAKHDSDKATMALDCFVSDLEGQELLRYTEYDRSVVDSKDAEALGSLYGNFLRMVAGGLGWLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYH3</accession>
    <name>A0ABQ5RYH3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005241</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="58"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="124"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="221"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="240"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="283"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="296" mass="32444" checksum="A9EB8BED90B4BFEF" modified="2025-10-08" version="1">MEEEYEGSYEGVGGIASDRAAREDTEVIDLFQAVKENDVESVQIALDRGADIEQTSSGGVTPLIQAVQSGSVQVVEILLQMGADVHAATRSGDTALHWAAYVGNAAMTRLLLEGGANVDAVGELGNRPLHVAASKGHEQVVGLLLTHNAHTSFKNAYGNTALTLASSQRVQTWIKRVTEGGAAERAKLATELASADAETEAKVAERRAKEDAEAKLREERVAAARKKRDEEDAEEDRLEEMERARLKAEEEERRRLEEERIRAEEEARRLAEEEAKRAAAEARRKKREAAKKKKGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYM9</accession>
    <name>A0ABQ5RYM9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="121" mass="11886" checksum="87E73454F822B570" modified="2025-10-08" version="1" fragment="single">AAGVPPTARLPPRAGSLLQPKTTVATAAAAAAAANTGGITPASPQPLARWDPLKMRQRLPAGHGCLHACALGTAEPLAPGAWIPTSMMLPLAVLLQALAADGDGGGGGGLDYVPPSVADVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYU5</accession>
    <name>A0ABQ5RYU5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Naphthoate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_005551</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-succinylbenzoyl-CoA + H(+) = 1,4-dihydroxy-2-naphthoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:26562"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57364"/>
            <dbReference type="ChEBI" id="CHEBI:58897"/>
            <dbReference type="EC" id="4.1.3.36"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01934">
        <property type="entry name" value="MenB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010198">
        <property type="entry name" value="DHNA-CoA_synthase_MenB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01929">
        <property type="entry name" value="menB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005637">
        <property type="entry name" value="PRK07396.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43113:SF1">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOYL-COA SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43113">
        <property type="entry name" value="NUCLEOSIDE-DIPHOSPHATE-SUGAR EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="37027" checksum="A4C35EF162B2C651" modified="2025-10-08" version="1">MSPQKRLQILGNHLMGSRPENLILRQATTATDKPEVTVPYASADGKVNSYGRIHGPVSRKPANWQRIPVVSREELTDVVYEKAADEGIAKITINRPDRRNAFRPRTVQELSWCFSDARDDPDVGVIILTGAGTLAFCSGGDQSVRGQGGYVGADGIARLNVLDLQIQIRRLPKPVIAMVAGYAVGGGHILHMVCDLTIAADNAVFGQTGPKVGSFDAGYGSTHMARLVGQKKAREIWFLCRLYNAQQAHEMGLVNTVVPLEKLEEETLVWCREMLRNSPTSLRVLKAALNAAEDGQAGIQELGGNATMLFYQSEEGNEGRTAYLEKRPPDFSKFKRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYZ9</accession>
    <name>A0ABQ5RYZ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">adenylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_005580</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="119"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="580"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="670"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="764"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="882"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1199"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1317"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="777"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1199"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1215"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1231"/>
            <end position="1240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1351" mass="134653" checksum="FD07ECA6CF99983C" modified="2025-10-08" version="1">MKKNIFIYPCDSFVGRSVANAFSAAGHAVSGLAIEGLNIPSSVKTAIRVTDPDAAIAQKQLLLTADVVIYDLLGNERATRDAVVQLTQQPYGGREVLFVAVSSPLVWAATRPNPSTLFAAAAGPGGGPGGGATGSSGEWGGGEGQGGGADEDGMGVDGLDDAPWPGAGGPNGSGAGTGSGYGDELSAPDSALGAPVSDSGREDRPFSPLAVAPTFRAPEDVPRRLPAATARAALAVEHLVLRAGRRGKLRTVVVCPGLLYGGGEDDLAFCSAFKAAWTWQCRSTRGFASAIAAATAADGSNGGTEPAIEHGSAPPRLLVYGRGTNVIPTLHVADLAAYLMALAGVGTGAGVEGQAPGMTVAAAAGGAAGAATGCYATSTPPNAVANGAFSALMGSTFGFGGSAAGGTSSGGNSNYLLVTDGSRFTQMELMAAIAAALGVGPEEGAIQTIPETELHLHPAPLRSRMLLDLSLVTTPLPPPNPSAPPPVFSLSRLGGLTAHLPDVVTEFRNARNLNPLRLLVTGPPLSGKTHLARRLAHRYGLTYINVPLLLAAAADSTVAVAAGLSPSLDPSLLRQAQLEVAAGPSSSPNPKEKEKEKEGPNPGWLPTGGRVSARTLGALLAAALADPRCAARCRGFVLDGYPRSAAQARAAFYTLQVDEAAMAAMAAEQSHAKLGPHKSHRGDRGGDRAASLVATPSTPNVAAALTATLGGCADILDVPPAMKLVPNPLTAPTHVIELDVTEAELRARLAAVIAAADEAVTRVTGEPSTLSDDKKAPGGPVAKKPASTAGVPGGKGGLAAGVVPRDLTLASALGHNSEAGFARRMAAYTGWRAEDSADLRSRVAAAQAAWEAEVTRQEAEKARRAAEAGTLKAKRRRARATAAAAVTGTESPEGDEDDVAAGNIPDRGLVVTAAAASSPEMPQHGGLMALSLDAAGATHVRLSNPTLPLGPIVTIAVQPPPQAALIAMGAAAATSGNAGPTSIAASVSAAAAQVLSSPPAPPTPPLVLPERAVEVVMGPPHNLVGFPEPGPTTVQSDAAVATEPRAAVVTTAPTTAPPPPSSLAAAAAAAGAPPPAMFVTATGPLKAGLPPPQFVSSAVIERADAALPGATAITAAPDTDFKTAESWTPRSSLSPLPPVAAAAAETMTVQVEAVAAGAEASYGYRWRGSRGFAVRRYLLRRVIPVVTAALAEVALARTGSSVPPGSVGLVQSSGGSFGQAPGGGGSGSGSAPGSRAGTPGEVPSQLAAAAVAAAATVAASGAQGAAPGPGSTGKDALLHVARALAAAAAEAEAAFQDPYSDPSYAIQLAKIDAKAAREAARAASAAAKAEREAAARQHVHPSNEEQQQQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ07</accession>
    <name>A0ABQ5RZ07_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heat shock protein 70B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">HSP70b</name>
        <name evidence="6" type="ORF">VaNZ11_005592</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10234">
        <property type="entry name" value="ASKHA_NBD_HSP70_DnaK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003520">
        <property type="entry name" value="PRK05183.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="639"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="673" mass="71986" checksum="65286C683DA1253A" modified="2025-10-08" version="1">MNSSRNYSVSGPAAGTSVPVLKNSKMNIRRRALAVSVRAEKVVGIDLGTTNSAVAAMEGGKPTIITNAEGGRTTPSVVAFTKSGDRLVGQIAKRQAVVNPENTFFSVKRFIGRRMSEVGQESKQVPYQVIEDGGNVKIKCPNALKDFAPEEISAQVLRKLTADASKFLNDKVEKAVITVPAYFNDSQRQATKDAGKIAGLEVLRIINEPTAASLAYGFDKKANETILVFDLGGGTFDVSVLEVGDGVFEVLSTSGDTHLGGDDFDKRIVDFLADEFQRSEGIELRKDRQALQRLTEAAEKAKIELSSLTQTSINLPFITATADGPKHIDTQLSRAKFEEMCSDLLERCKVPVQQQALRDAKLSISDIQEVILVGGSTRIPAVQDIVRKLTGGKEPNVSVNPDEVVALGAAVQAGVLAGEVSDIVLLDVTPLSLGLETLGGVMTKLIPRNTTLPTSKSEVFSTAADGQTSVEINVLQGEREFARDNKSLGTFRLDGIPPAPRGVPQIEVKFDIDANGILSVTATDKGTGKKQDIRITGASTLDKTDVDRMVKEAEKFATEDKKRRESVETKNQAETMVYQTEKQLKEFEGKVPADTKAKVESKLADLKSSLGSEDAEVIKTAMTALQQEVMAMGQAMYSQAGAEPGTGPQQGAGGASSSGSKNGDVIDAEFSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S002</accession>
    <name>A0ABQ5S002_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_005280</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16449">
        <property type="entry name" value="RING-HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14447">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016965">
        <property type="entry name" value="Pase_PHOSPHO-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06888">
        <property type="entry name" value="Put_Phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="194"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="7">
        <location>
            <begin position="350"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="556"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="590"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="923" mass="99246" checksum="DE68801238337702" modified="2025-10-08" version="1">MKFAKRLASEASRKVSFAAAYFDYRAAKKAIKDDIHKSDVAGSSFQELLESELRKVSQFYSEAADRIEATLQSLGGPSGGGSAQQLSVVRAEIKELIKFVALNYLAVVKAIKKRNRHLKETFGASASISLQALDMLGHEVFFTSPRLAHLATQADVLSSASGLAASAAAGACCVRAAAIAQPTEAGAVLEDYQCPICLDTLHNPVVLTCAHRFCWGCLVAHVTAVRDQQLPLAPAVQDGKATDAGSSATNSLQLLERIAAQDDSEGVQRFYNCPVCRKPQLLDVDSLSVDPFLSSFIESLKVLSVNGGPGAGVTAAMAPQAPVLPPSTVTKKAASPSERAWGILPPQRPEHAGRLCVLLDLDGTLVSSYTPRRAPRLPPYVRTHVVGMGSKLNPGGVFVVERPGLREFLEELSSFAEVVIFTAGLEDYAKPIVDALDPRGNLFAHRIYREGTLRTEYYQCVKDMARVGRDLRRTVLVDDTPLAFLHQPDNGVPVLGFRGDPDDRLLQEAVLPLMQVLSKEADVRHVLQRRFDMTTWFRRNSFPIDTILEQAKELARTERERHLGSGGCAATASLPPPASPMKPGTPMATGPSSLRSGTSDGRSSRCNKDSSNGRCYTFTGPRQPAPTKRFALVTDFDKTLTDFDAGETIVERLAPELLPMLIGLDARQSYIPITNTILSEIQRRGVSRDQLLTALQQLGGELPLASVELLRMMRGASIDVKILSDCNSVFIGHILAGAKLKGHVQEVITNPAAFEWVAAAAPDGAGAAEGTAAALQRTAGQRLVILPHYDPNDASVVIAAVGQSTCNSGSGQCNCKGGAAGSRPRPHGCPRCPDNLCKGLEVERLLHANVYNTLVYCGDGANDVCAALALRPQDWLLARAGHPLAAYIEEARSNAAQPQVVARMAVWNTHEELLVCVRQVVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0R9</accession>
    <name>A0ABQ5S0R9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ribosomal protein L7Ae</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">L7Ae</name>
        <name evidence="8" type="ORF">VaNZ11_005607</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
            <location evidence="1 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21104">
        <property type="entry name" value="SNU13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="7">
        <location>
            <begin position="22"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="13625" checksum="B41EA5BBFA5C7A59" modified="2025-10-08" version="1">MSEAVNPKAYPLADAQLSNTILDIVQQAANYKQLKKGANEATKTLNRGISEFVVMAADTEPIEILLHLPLLAEDKNVPYVFVPSKAALGRACGVSRPVIAASVTTNEGSQLKPQIQNLKGSIEKLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Z1</accession>
    <name>A0ABQ5S0Z1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">U3 small nucleolar RNA-associated protein 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_005782</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat UTP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045161">
        <property type="entry name" value="Utp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359">
        <property type="entry name" value="WD-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="418"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="59"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="614" mass="65906" checksum="045B43993A037F0F" modified="2025-10-08" version="1">MSKAKKQRIVYKQDGKAKAAELEEFLFGVGGNPLSIFDREKEEAEETVAELVTQASRRGRLVHDSDIFFQDTRGAVGSGSSDDSSSSEEEEGQEDEEEEGDDGKQKDATDAKGREMEMEEEEDGEQLQEKAAGRNGVGARKGRTAAASSRGGAGGRGKSGRRVPVWNDPDDVNLRMDVAGTARLRKLRTSEKQTTMDGVEYEAALRRQHQILNPRTSWAKKTAAGGDEDEAAALLARAGGLVERPVGQLLPAGQIETSRLKDANQHQPNDSVVQSVRFHPNGQVLFTAGLDKKLRLFQVDGLRNPLIQALHFDDMPITHAEFAGVSSGSSDAAAAAAAADRVVLAGRRPYFYVYDMGAARVERVLGPAGCPQKSLEIFAVSPPGTASRSPSSDPLVAFTGDGGFIPLVSLRSRQWVANLKMSGNVRALAFSHDGVELFSTGDDGVVHVWDLRTRRCRLAFNDTGNIGDASTIALSADGRFIATGAKSGVVNVYQRGQVEAAAARAAASGGGGGGRPFGPVSVTPDRELMHLTTTVDTLVFSPDSQMLVMASHDKRNSLRLVHLPSLTVFANWPTSGSPLHYVHCADFSPNCGMLTIGNARGRALLYRLHHYQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S193</accession>
    <name>A0ABQ5S193_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42103:SF2">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42103">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="43026" checksum="3847B05637CDD549" modified="2025-10-08" version="1">MVRQLVPLPSRGVSLEVDIHFIEPKSTSRSGLLLVVLHPWAWLGGCMDDHVVAAVCRSALDTRRFCCVVRYNMRGAGASGGIRALCTSVDAQDLVVLAHELLLQQQERQEQHPSGQLQQRPDSVPASAQPTDGIPAAQPRLVLVAYSYGSLVAARALPQLRPHVAAIAVLAPPIGSLGRVMLGAGSSWGALAGARSTVRDIPRLVCCGDRDQFVSVSQLRNAVQQALRAEAQETTKAAVAVEASAARATSHTLAGVATTTYPPAAQLASESTLELVLWPDCDHFFFDCRDAGALEAAGLLGRYGPGTTTTSPFVGGVGGSAGGGVAHGSRDRDGAVGRSVAIGRDNRGIEAAGCSTSASNGSCTGASNDAAAEARVKGREKLRPRHRVKLSKALAEFVMGWLLAKAAEPEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1M5</accession>
    <name>A0ABQ5S1M5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <ecNumber evidence="4">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006401</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020624">
        <property type="entry name" value="Schiff_base-form_aldolases_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF15">
        <property type="entry name" value="4-HYDROXY-TETRAHYDRODIPICOLINATE SYNTHASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00665">
        <property type="entry name" value="DHDPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="352" mass="37917" checksum="F1DF354401045169" modified="2025-10-08" version="1">MAPHSSLISRGKVSRCAVGRRLTSRLCAVTVLAVLAPHPASETRSAVDRLKKIRLVTAIKTPYLSNGKFDLPSYDALVSRQIANGVEGLIVGGTTGEGHLMSWDEHIMLIAHTVNAFGDKVAVIGNTGSNSTREALHATEQGFAVGMHASLQINPYYGKTSKAGLLNHFSAVLQEGPAIIYNVPGRTGQDIPDDVALEISQHSNFLGMKECTGNVRIKNYVSRDVNCWSGNDDEAHDARHGSGAVGVISVTSNVVPGLMHQLMHGSNPEPALNASLKELFAWLFCEPNPIALNTALAMCGLVKPVFRLPYVPLNRQQREKGAALLMAVQEHIPGCSGVRVMEDHEFILIGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1N0</accession>
    <name>A0ABQ5S1N0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006907</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051246">
        <property type="entry name" value="WDR48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862:SF14">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2478"/>
            <end position="2497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2518"/>
            <end position="2537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2581"/>
            <end position="2604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2948"/>
            <end position="2971"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3012"/>
            <end position="3035"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3118"/>
            <end position="3141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3153"/>
            <end position="3175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1482"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1842"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2625"/>
            <end position="2711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2723"/>
            <end position="2757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2816"/>
            <end position="2847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3222"/>
            <end position="3244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1492"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1842"/>
            <end position="1862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2663"/>
            <end position="2679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2723"/>
            <end position="2732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2739"/>
            <end position="2750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3244" mass="342739" checksum="EF43EC14851B31B1" modified="2025-10-08" version="1">MPIKFKVKPTLSTKKLIIHLGEFNVNPCTMGWRQDRLLQSFWIVVLLSGVLQWSSSVYAAESILPGATVVCSITLTGEPKSTTSINTGAWMPAFFRIQCNSTGHSQTRVTIGLGEQLMRYAPPADGKCGSPPSSPTSNAGSPPPYPVFPLSPSYSDAPSYPPSYVGAPVPPPSYRWATPSPIYLSGTAATLPSVSRTGAGGGAWRETHSTVSLPASEGSTDSFDALSPTSPISPPLIPPSVTPPPFPLMVGAECFVESWDDKDWGITFTDQIEHLELRDSVIQGAPLSYQPLIRCPNCKYVTLVNVTVRDLQGKNLSIDDDCEGRTVGALYFGGVRGAIVDQLTCVNVKGATDFACLWFGLTSTDVDSGNEALSEAAFFRISNSAFMNNSITKGDCIEDDGSEGFGFGCVVFYAIDADALLGSVEVFGTTMYDIEGGFGSAVSFVTRDGNLLHMDVFNISGSNFTHNKAAKHGGAVFINVRTSLDLLHVTQSSRIDNNTAGSFGNGGAFYIGPLIESLVVEGTSSVSANSADLSGGAFYFEKGITNISILRGSNVSGNRAFENGGAFCIRDADVNSISVEDNSHVDHNTVDVDVGLVSNGGGAFYLQDVSLKQFVLLNGSSMDNNMLIGTAATRRPGSGGALRVDGDLDTLVVRQNSSISNNTAGAGGGVFIARGIPGGIQVLEGSRIDNNSATGGDVDVQKPDGGGIMVTLDCPNVTVMDWSSISGNIASHMAARGGGVFIGGGLQKLVVKKGSKMSRNFATQGGAAIGVGLSPDLPIGDGAYMVTDLVEVSDESCICNNSASASNIGSYSGAMYFGKTRVNSFTITNGSCVCGNKVAFGDGYGGAVYADNGFVNFTVCHNSGLSENMGGSGGAVYFPDQIGQPFLSLERFELCDNSHMDGNQARTSGGALYMAGTVGQIIFSNSTISHNRAMITGGALALRSMFQSFSIGDALVYNNSVKFAQGGFLSLEMAPDDITTTSKSEVDDPTAADLSKYHLPSNGPFYFNIIRTNISGNSAMKDGGAIAFDLRIMMSNIYNDTLRTQLQKGQVLYIFITDTSWNDNWAHYGAGGALAFLSITPFIADEQRLASVLLGARINIKNSTFTTNTAGDNMKRFLSVKDISPLRGNGGALFIWAEPFPFSNVDAGTLSSNQSAYVMYDDASNCVTTDMDDSQNLSCWPRGSQSCGIRLEGVTLTGNLATGGYGGGLLVTHCAAKIINSTFYNNSATLDGGGLAFMDYALSAYLDMILHQSIRDSRMPPNATTVLPLPTPSLAGGLRDNYVLSSSPGMITSGDIFVLPWLDNTSSVQPSVEALQNIDTSKPLQQPWLVLTHTSFEGNMATNGGAAFLEVNATAAVIIDCDLMRNQAQVQGGGIMLVCASSFGQFAAIVRGTNFMHNDAGTAGGGLSARLTRTWAVAVLLNGTFEENSATRGGGIFNSGVKGSSFLLWSCTLQRNTANFGGGIALDYLLLSGTSTASVVTPSKNNVSGLLPPSPRPPSPPPAPPRPPPPPAPPPPVNGIAAFNPSHARVHLLNCNLRDNQGILNGGAVYMTADKQNVLALLEQSKFGLNRAILGGGFYVSLTRACVMRVSNCSVLDNTAQLEGGGAYSTTKCGGQLLVGNGSVWSGNSAGSYGGAIMVVNADDITFSNNTANVSLPGTMEPQPCSLVSLNVSDSSITGNNAQDGGGIYAYNETSILVLGSTLANNTVSRSGGAIAVVNCNLLNLFNCSIANNIAAFAGGGIFNDKCAIFVMTLGDFIQNRASTGGGIHIVGNTVPAIDSGVINDALGQRRSMQSSGEVGTYTSGNATGGTVDNSVAILSLVDFDRNMASADFGQLIIKDGAASSETPSPSSSPSTATAASSNLQSYPGHGGAIFISGHVGVAVSNSTAGPGNYANVGTVLATTQACAWATLNATRVSDTTDKTAVEKVAMIVATDLVQEVRTLSRSAREQCSLLMLSETKLLSATTPQQNSSSKLPLIWMRDLNASALLVNCAATGSRSINGDLINKTQQEYLQHVPPPAPNPAAETNNGGNANLPPFLKRMLSTCGNSSGQHGAAMALAVPATHMRLISFGGVLLVPTKGVQVLRSAGYSTFDAEDIKALVVKPNTFFDLGVQLYDGLDQPVFVDTPTFSVSLSIHPDSTDAILRSEGISLKIVTINGEASWTALEILGWPGKYVLEVTAVIDESSVNSTLPEISPLKIGVELLPCEVGSELVKGDGNGYSCSACRRDRVGLWTDRRPPLSKLPSHGNNLTNLMNSMYVDLTIGAADGDEESSCPSCCQACPTNAICPGGALLLPAPGYWHSAPNSPRMHRCPQPAACGKVESFGDALDGFWDKALLRFNLSSNGLGPLPTVTFNSTIIAVDNLSVIALRKDNRSLLLTVCQLWSYETFPPNRDNVLQQQYKIPRLTMEEELPCVLFKDDPSTSSTRPQGSSGNRSYLQLQCATGYTGHLCAACLPGYSLSVDFSCMKCPSLARTIVVGLLAFWGTVALILFTTFSNMSLTRSETMEAEEFSSLDLLKTLITHVQYFIIITKLGIDYPPIITKYQSVLSSFTGSENFIAYSPSCLFNDLASASQAATQIAFGFAAPCTAVVVSFVIWGFRYVMANQSKFRRINASRFRGSPRLTHEIPISMEPSQQLSKSGSKEGPFQITRDSDLPSKANKTAGKGHPAATAATTTHGIRRLVPSTAEPMLPSVNSSPSTVAREYKQSDPRVIAERQQNILSQGHSTEPGTSCRPDPASATTSSTSPSSGPPLLCGVQAESEVAIALAPPGVDATVATAASPPPANTAPVSPLATRRTFNSSLRSGNNHALLDRQTAVPSGPGSPPSGGNGIQHNGVMGAPCLPHSASLPHEPDCAAGYPIPPPAAAGNKPMSREEHWKPTPFSLTTFGPEDGFDSDILAQAEENKDRDQEHENACSFIVRAVQRYANPLHLLLFADQSVSMLQQLGVVLIVASFILYPSLCQISLSIFSCYKLDPGTGVFKENQQATWSHGYWVRNMQQECYAGVHKRVYVPIGIATVLLFCFCPPITYLILTTRCRRKPNDTRMRIQYGFLYQQYKPEFFWWASMRQLQVLALVAVEVFGRGLPVQQQALMLLSVLIVIAAINTSSSPERFHELLILEFLSMCVLSLTLTLGLYFIADGEDQLSATASTAVGAVILTINACFILGVLTLFVRGSADRARQVHRILSTSWNSVVTSVSRAIVHIEQRYSMASTPGIVTARSSQRSATAGRVGSASSGGNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1S3</accession>
    <name>A0ABQ5S1S3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_006555</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF35">
        <property type="entry name" value="PROTEIN O-GLUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="4">
        <location>
            <begin position="11"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="287"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="371" mass="41735" checksum="9BF0013E19C6F8D5" modified="2025-10-08" version="1">MLSIVLYLYPQMPDMEFLWLWDDECVNGLPVFAPNACRQFAKAGFTLPSFSVWSQSLGRVQMAAYHSCVWNRYPPYERKPLAVWRGSTTGARKPTVENYQLVTRLKLHLLAQNHSGILDAKINKFTKDIPDPVRAQITSGDRIDSEDYNRYSAIIDVDGNGWSDRFGHALIHYSTPVLKMASNHTAFFEHLFAPGVTFLQFSPSLEDLVEKAGKVVAEVQKLGGQSKYFDMVKSMQATSQVLMDHLGLVEALAYSLLKYWSLVTWTLKDLDPDTDVDLGHRNRLDPSIVKRGGDRAGGVGQDGGSDDDDHDDDYTKASAKPLTEFPDEVRGFEEVAMTCCSLTRVPAEFAAEVTTRLLRKAPGRSFFFWVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1S8</accession>
    <name>A0ABQ5S1S8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006936</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="167" mass="18876" checksum="7B49521F269CCAEE" modified="2025-10-08" version="1" fragment="single">ERWRRRSTFPSGTSKSKLLKSSCDAATRAAVAVVRRALGPRAFVAFDASPYAVAKTLVLERMLRTGGPAATDLTLQVWFSATWTSDAQTAFWRTLTELWAENRHGGRKGGGNMPTAVLSYFHHWLLRDVSLTESRQRWFSEWKRRLYGEVGQFRDNVPKADGEREAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1U5</accession>
    <name>A0ABQ5S1U5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_006374</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="484"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="546"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="644"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="676"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="7">
        <location>
            <begin position="112"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="8">
        <location>
            <begin position="796"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="735"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1004"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1027"/>
            <end position="1038"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI63409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1038" mass="107891" checksum="26A9AE75A733701F" modified="2025-10-08" version="1">MNCRTIRLTPTVGLKYVKQHAPVPTIVLPGRPRSCTEPHLGPLSGICRRPMKIRAQAMQAAQAVGGLIHVSETWIMAGSAALALSVPVALGAVRQGRVQSVRPGDALVLLKTGGNVKLVDVRSRLEVKIEGLPLKRNARKAKVIHAPFNELHGGKLQPSPSFLDDILFATGVDQETTFIVLSSRGSETCRRAAKAISSLSDRHDIYMVEGGFRSWQAAGLDTEAAGSFGEDSSADDQEDYYGDLYDKEARQGSAGGARRGVEEVFLDSAESRGLMGMLLRVPFKPKLLEVLKQGYTADDLRRDVLAGLVVGVIALALGMALGIASDSTPANGLYTVVVGGFLASALSGSRVTVVGPTAAFIPIVAGVAHAHGAVGLVACTGLAGLMLIAMGATGMGGIIRYVPKPVVTGFTAGIATYIFGTQIKDFLGLGAPGRLPEGVPVPTEFLEKVVYLGSHLDAANVPSVAMALGCLVLIKAWPKEWAKVVPTPIVAIAAGTLGLQLLQLLQPGGELGIETIGSHFGAGAIPRSLPSLQWPQGLSPSDVLGLLYPAATIATLAAIESLLCARVADTMIGDKHDSNTELISQGIANVGCAAFGCLPATGALARTAANIRAGGRSPVSGLVHAATVAAVILAAAPYAEYIPLPALSAVLVVVAINMGEWHNFQQLPKWPQNDAHLFLIAFSLTVLQDVAVAVAFGMVIALAVFVQDVSATMYVRTLPPRSVIVPQYMDTAAGVLDNDDADEPYRRRAPAARRGSSGEDEDPVEGPLPAAATDGGASSGLLTGGTLGSVVPPYTVYVEVTGSLLFGAGEMLERAVETEGGATPTQEAVQVLVLNMSHVSVVDVSGLELLEEVHRRLAEAGKTLVLCGLTRQPLRMMARAGFLDEAGRENVCRGVDAAFARAIAIVSANRMKQQQLQNNKDSGSGHGNGSTAADKVTTLASRTTGHLDGYPGVRAVTAVALGSDDLEELWTKAAVPPSVSFQAASGRLQDDGFIRANNIAIGSASLEPPSGNGAMSRRGSGGGNMDLEVRDEEHGGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2D0</accession>
    <name>A0ABQ5S2D0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007194</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="6">
        <location>
            <begin position="281"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="405"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="452"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="527"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="410"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="563"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="583" mass="63656" checksum="61BF242E26DCF395" modified="2025-10-08" version="1">MLLLFETAAGYALFKVLKERKIEEAEDLASDFQTLEQAQKIVKLKAFSKFENTTEALGAATALVDSKLSKGLKKFLKKNVSEDEKLAMLDKKLGSIVQEKLGLNVLWSNQVLELSRGIRNQLTGLISGLASLDLRPMSLGLSHSLSRYKLKFSPDKVDTMIVQAIGLLDDLDKELNTYAMRVREWYGWHFPEMTKIVTDNIHYAKAVVFMGTRDLAQGLDFSGILEEDVEGQLKAAAQVSMGTDISESDLDNIKDLANQVIALSEYRSQLFEYLRNRMSAVAPNLTVLVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHETPKYGLIYHASLIGQSAPKYKGKISRVLAAKCALAIRVDALGDSNDATVGMEARQKVEARLRQLEGKLLGTEAGAAKGKEQPAKYDKSRQGAVPALATQPKAYNPAADVADVANGTLAEKKDKKEKKKVTAAEAPAPEEGEEPKEEAKVVVEEVTAADGEKVKKKKKKAAIEEALEVVEEPKKKAKIVVEEVTAAEGENVKKKKKKAAIEEALEEPKKKKAKVADGGANGKEEKPTKDKDGKKKKKKAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2S7</accession>
    <name>A0ABQ5S2S7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006637</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="66"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="175" mass="19674" checksum="FD9B10CF5D560E6E" modified="2025-10-08" version="1">MDTDLNSNLGTKLRSKNSVSELLKFSMDDKRPPKNIFEAAQRNDTGYIKKLAERTLDFNVNTRDQLQRTGLHWAAELGHVDAAELLIDYGIDVKAVECNGRTAVHLAARSGDRAMLECIMELLSPEERTEMINQADNFGVTPLYLARQKDKDGQDAFEYMIVNGGRINEEAKKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3A9</accession>
    <name>A0ABQ5S3A9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PI31 proteasome regulator C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007661</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the proteasome inhibitor PI31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045128">
        <property type="entry name" value="PI31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013886">
        <property type="entry name" value="PI31_Prot_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13266">
        <property type="entry name" value="PROTEASOME INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13266:SF1">
        <property type="entry name" value="PROTEASOME INHIBITOR PI31 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08577">
        <property type="entry name" value="PI31_Prot_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI31 proteasome regulator C-terminal" evidence="5">
        <location>
            <begin position="317"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="126"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="221"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="223"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="267"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="436" mass="45205" checksum="6DA65BEFB3255F68" modified="2025-10-08" version="1">MATPQALTAVIRASRPDFTSTADKAAFAVHAILSVNGFSLRRVGKDVDASVEQGFALLPGEEVDLGNWNQEAQQADGGMYSFLYVPESGGAQRPTVQQQQDKATPQPQPRPPVLLVKCLRLAVEPPVAGTTGGGDSRDAKAGDVNVSRADGFGGDGGGSGDGPRAGGVLLISLADVSSGHPPVSLELQLDRYVSSLAPGGAGYEHLDELIRRVQEALEEALDGAEASSSRAVGATSTSSAADRASRGGRSSTAAAAAAAAAEAVQRQQEEAEEASRTLRQAVRDEEGRQRRDPLRDERYPQPVFPSRMPPGWGPIGVGDEDLMPGGLPRPPGLGGLVGPGGGGPRGLGGGMHVGPENPIFADRLRHPRGGPGFPPGVIGSGGALPGMRWDPIAPEGLQGWAPDDYTRGGRTQHDEGLNDIGRPQPGRGPYWDNMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3J3</accession>
    <name>A0ABQ5S3J3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007569</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026054">
        <property type="entry name" value="Nucleoporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23193">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23193:SF23">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047127310" description="Peptidase M11 gametolysin domain-containing protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="6">
        <location>
            <begin position="134"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="477"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="481"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="747"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="761" mass="79282" checksum="474E4523F8E29DC7" modified="2025-10-08" version="1">MRQRSVFVILFLTTILVTPSSAGKDDAPGQVKKQQQIQGELVYVTLDGPTQQWAVRDAVGQLTPVDRTTFKAPKKDKDGKNDFQSGTLVSINCDIGDDGLCTPAAAVGADAATVLGPTPTAVAVTATIPATYQRVLVMIMDYSACGLAPGINETTVRSLFLGPNGDGLGGVAQKYTQCSYGRFNLNTTAFRVVTVPQNCSAAVTSSCSWWAISNLADTATKTLIGTTAFSDFTHYIYVVPPGLNSVCPWAGLALLPGKQVWLQTSSYGIYRWATIMQEAIHNYGLWHSWQNGVEYEDYSTAMGRGDACPNAAEASRLGWSSAVLGGDSINSSVLTQAGVVRSFTVPASYLTGDNNYLRVKPTWMPMYSDITLAKNLYIDVRVAKGADAGLANYTAGASYYPKVNVHEVNATMDNSYPALYANGDRKITIINLIPSLSRANLTSYNLVVYGGSWASTDIMRVHLCRYISSPAECPALSAIEAPPPSPPPSPSPPQPSPNPPPSPKPSPPPSKPPPPAKSPPRPPPLPPSPAPSPLPPSPKPPSPPPPSPPSPPPPSPPSPPPPRPPSPPPPSPPSPPTPSPPSPPPPSPPSPPPPSPPSPPPPSPPSPPPPRPPSPPPPSPPSPPSPSPPFPSPPPPSPLPPSPPPPSPVPPSAPSPPAAPWPPSPPSPSSPPPPPPPPPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPAPPSPPPPSPRPPHPPPPSPRPPRPSSPPPRPPPKPPSPRKTRTVRRRHLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3S8</accession>
    <name>A0ABQ5S3S8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003832">
        <property type="entry name" value="DUF212"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31446">
        <property type="entry name" value="ACID PHOSPHATASE/VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31446:SF29">
        <property type="entry name" value="ACID PHOSPHATASE_VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02681">
        <property type="entry name" value="DUF212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="265" mass="28179" checksum="A380AA5A3B894B31" modified="2025-10-08" version="1">MQTCGMQLKATRSVGSGKRRVGQASAGRRTEPTLAHCCNARQAASFGTSTSCSTVQLPIGHALGLLEGARRAVILKALKAHRAIRRELKTDKPSGSATGPHPGLLMLSALAIPPIPGNGMIDLVTNRVFLVGFWSWFTAQFMKIFTKRFKKGVWDLGAVLESGGMPSSHSSLCAGITTAIAIQQGFGSPLFAACLCFSVIVMYDAMGVRRHAGKQAEVLNKVIDELLDDDHPMGEVKLKEVLGHTPRQVICGGLLGLAVGLFFPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3Z0</accession>
    <name>A0ABQ5S3Z0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045160799" description="Glycosyltransferase 2-like domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="54312" checksum="F11F197702484F5C" modified="2025-10-08" version="1">MLIGNVIRGLAGLACVWLLGNLVRCNGDQQRLNRLPYCEEHLGKLPSNSSELHILRDLFQTWLINRRIEVDMEGLREKMRNPPTNIEEQYAWKTTWNESYVSHRIEKATFARTEIEAKLVSIIKNLREKGLIESTTNVQDLVHCTSGHLQPRLFPDQPLVSFMLQYFKRPRAIDKFVTMLQACNTLIPSEFLINVDSPQEGQRWADLSWSTHGFVIPVMSFNVHELRGYNRLASLARGKYMVFLQDDDIMEPADCSWLPRLVKQFEAMPSLAMIGLNRMQLSHGEGNELNAYNFVDPNTTNNIIFAFQVDLAPLVIRRSAYKFVGGLDEGMSDPGECGIWSDWEISIRFWTAGWHVAYMPLIHKAPSDPGEPGGTHKPETGVRCWGRQQGIASTFYMARWGAGWGTGPGKFLESLENFVCKGREVQQGGVALVHKGVLYQAGKPGQCVVADRLQRTAPGGGQGLCQLAGRVRGPLLHSVYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3Z4</accession>
    <name>A0ABQ5S3Z4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="5">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007907</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="597"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="609"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="622" mass="67475" checksum="317D350539CB5D1E" modified="2025-10-08" version="1">MVATAGASHMRQRLASGLLRTQPRRSISLKVAVCSMPIHPTSGSALRSLRVVVIGAGAAGLAAARELRSEGHDVTVLEQSPYVGGVWRYDPRTESEDLLGINSDRSRVHSSMYEQLRTNLPRELMSFIDFPFDSSLLGPQYSSDPRRFCSHTEVLGYLNAFADFFNLRPLVRTHTRVLSVEMADEAAAGMATGGAVRQAPSPPIQVTMATTTSSESLFGFVVTSERLGTPHHDKDSRGDSPAFDTGTRGGRRSLTAAPAAIEKEFYDAVVVCNGHYSEPRLPHVSGMFGESGAGAGGVQVSDAKGVFPGQQMHSHNYRCAKEWRGKVVLVVGASNSGEDITRELSAAGVRRVLLSAWSWKNDAWGTDTAPYGPGGNIYRYPMVSELHADGSATFTDGRREGPIDAVIYCTGYRYSFPFLRGAAAEVARVEDNCVGPLWLHMLPPGPLAPGLSFVGLPWKVVPFPQFQLQSKLIARLLSGRVQLPRRKQMEEDIEAHFEAMRAQQLPKRYAHMQGQQQFAYNDMLAKLCGPDVVPLPWWRAELNRLTGLCKRDRPDDYRNVELATISSDAAAALAAASADISSQVELLKGIRGGSEVANAAHGAEKATKDSSSTDSGPKKSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4E5</accession>
    <name>A0ABQ5S4E5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_007619</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="676"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="752"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="818"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="896"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="999"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="140"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="340"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="424"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="488"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="547"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1203"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="280"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="560"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1215"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1290"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI64373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1331" mass="142003" checksum="39DEC3237A5A6C9B" modified="2025-10-08" version="1" fragment="single">MKRLMQQLHEELNALRQQQDALLKQFVDMRRQDEEERRWVKERLETLNQKVQEATESASALNASALAAAASANAAAKAVGVTTKRAPGIAANSSGSLLKTSSFEFARNLRFPKEGSTGGFGSINLASGAPAGSSPLAAGSLGARDLSHGGSESEVDGLKFSLKSPRRRFQQPSSSSEEAEGSTPHAGVGPMGRSVARRTYLTEEIRRIGELPVGQGRSAAPEEKGAHGVDRKQVSAFAVAAAQAHAAEDLASTGQIDDIIVSGGIEKAQRRRGEAVLRQPGSSLSAEPSGSLQEAMPSFSMISDNLAAMGLNAAGSEGAGSGATAAGSAQNLSTISYGSSLPHSVSMGHSQGHANHHHHQYHHSHSHEHGHHYGHNFGHSNSFGGGGSNSRAFFPLGRNSHHGLPGAFGSVAMLFPSLRHGASADDLPVGTTIAQPPSPTRSPTRSPRRFAASGSSTPYAGVTGGGAAPSVGGQALGLDVARMSVSAGGAAAGGTAPSLVRDSSRSATVSDPETERVDAASTRHFKGISPRLVEIYRQRAEQSHWSLQRDSVQLMRGSSRRGSTDTMQRPTGPFRPAAAVVGSTTGIGSGMSRMSLLHGGGGGGGGGSSKAHDGLASAGVGGIDEASGWVLLDPNYDHPPMIGDDEEYSRPMSLVDRLKLYSVGLIRPEGRTKWDLFILVLLVWVLFASPIIICFGLAGDAFHGDWVGIVELCVDGAFAFDIYLNFRTAYYDSKGYLVTERWRIARHYLKTWFLLDLVCVIPYDLITAGTMGFLSMLKLLRVVRVGKVIRMIRMYRLLRVIRLPRILEQMEMFIDRGVLQVMAFVLSVCLLAHLSACIFFYMAYLDGLGPQTWVAAYGVQEADLATKYLTSLYWAFTTTATVGYGDITPKTDKEKIIAIFVMCLGVSLVGYVTSSITNIMAIKNAQQTNIAAKKQLVMDVLKTRAVPSEVSRRVYNYFDYVSSKMIKREEAGLLAELPFKLRARLLTSFFADTLARIPGISRLPPHVLIELVSQLKPHYFVEGDIVAIQGDYVDALYIVSEGLLEVRTYMFPADVEPWRIFEAVHKDELRYMPYSAEGRLKPRYFFGQAGVLHGGVWPATVIARNCCELYSLDREGLEHMLAVQPELQNMLGVPHTPVPASVAHAAFVSGPGVNALLPPADRAALLADFPLMDPAPQRGLMRSMLRSMGNIITGGLARASAAHYPNMPGSPTSAGSGGGSGGGGGGGGARSMLRGLMGWRKGGEAVGGARDADGHGVAQAQDLASGSVEPPPAESSFSRFRPFGRRGPRNTSVVQLLPSEASSLLHSRDERSDANGEAQQPVVGMDSAFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4R5</accession>
    <name>A0ABQ5S4R5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">xanthine dehydrogenase</fullName>
            <ecNumber evidence="5">1.17.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">VaNZ11_007923</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>hypoxanthine + NAD(+) + H2O = xanthine + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24670"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.17.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>xanthine + NAD(+) + H2O = urate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.17.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the xanthine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.4" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.120">
        <property type="entry name" value="[2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014307">
        <property type="entry name" value="Xanthine_DH_ssu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02963">
        <property type="entry name" value="xanthine_xdhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45444">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45444:SF3">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000127">
        <property type="entry name" value="Xanthine_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="CO dehydrogenase flavoprotein C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="CO dehydrogenase ISP C-domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="14" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="19">
        <location>
            <begin position="11"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="20">
        <location>
            <begin position="284"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="GLI64614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1392" mass="149359" checksum="2CED45610954A998" modified="2025-10-08" version="1">MVINETTSAPEPICYINGTRYVLPDGRGEATLLQFLREIGLTGTKLGCGEGGCGACTVMLSHWEDGHVVHRSANACLCPLYAVEGMQVVTVEGLGNVRDGLHPVQQRLAVMHGSQCGFCTPGFVMSMYSLLRSCETPPSEEDIEDALGGNLCRCTGYRPILDAFKTFAKTDPAAYTEEAIAASKGIANGSATSATTTNGASSGVCPSSCLPCDCAAAKATTNGGATTNGCGTAVATTNGCGAANCCKKARAGGGCDVAPAASRPTSEPIFPPELKTRPAFHLAVSGRNVTWHRPATLEQLLDLKSAHPAAKIVVGNTEVGIEMKFKNASYPVMIAPTHVKEMNQIAVTETGVEIGAAVTLTRMMKAFKGLIATRPRHQVSALEAVVNQLRWFAGNQIRNVSALGGNIVTGSPISDLNPLWMAARTTFVALGKDTGERAVPASEFFVGYRQVDLRPHEILFKIVLPFTRPNEYVKEFKQSPRREDDIAIVNAGMRVKLAPGASEGVWVVEEAAVAFGGVAPRAIMAPALAAALVGRAWDQDTLQAALAAVRQDVVLVDHAPGGKVEYRRALAASFVFKFFVHAALQLEADTAAAYQANLPTEEHSAAKAYERHPAHGVQFYSKPEAPPTPATVSVVGQPHPHMAAELQVSGEATYTDDIKLTQDALVAALVTSAKPHARITKLDATAALQMPGVVGFYSAKDVPGSNAIGPVWYDEEVFATSEVTAVGQVVGVVVGTTEAAARAGARAVEVGYEDLPAVMSIEEAIEAGAFYDDYTGKLECGDVDGAWTQCDHVITGTYKVGGQEHFYLEPNNCVVIPHENDEFTLYSSTQAPAKHQKYVASVLGIPAHKVVSKTKRLGGGFGGKETRGIFLHCAAAVPSYHLKRPVRLCLDRDEDMQMTGQRHAFLATYKVGFSADGRVLAAELDLYNNAGNSHDLSHSIMDRALLHSDCVYKVPNMRVRGHMCRTNQASNTAFRGFGGPQGLMFAEMWIEQIAKTLGKPDVEIRTLNMYDEGDVTHFGQVLEHCRARACWQTVLSSSSFWERKAQVAEFNKANRWRKRGLAATPTKFGISFTTKFLNQAGALVHVYLDGTVLVTHGGVEMGQGLHTKMAQVAAQALNVPLSKVFISETSTDKVPNASPTAASASSDMYGGAVLDACRQLSERLAPYRTKLPNGTWKEVVNAAYLDRVDLSAHGFYSTPDITGFGGRRPFNYFCFGAAVSEVEIDVLTGDMQVLRSDLVMDVGNPLNPAIDIGQVEGGFVQGMGWLVLEELMWGDKQHPWIRPGHLFTKGPGTYKIPSVNDIPVDFRVQLLANAPNVRAIHSSKAVGEPPFHLGASVFFALKEAVYAAREAAGVRGYFVLDAPATPERLRLLCSDQIVQPYVPYDVRPKISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5D6</accession>
    <name>A0ABQ5S5D6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glycerol kinase</fullName>
            <ecNumber evidence="2">2.7.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_007831</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07781">
        <property type="entry name" value="ASKHA_NBD_FGGY_L-RBK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005929">
        <property type="entry name" value="Ribulokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435:SF4">
        <property type="entry name" value="FGGY CARBOHYDRATE KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435">
        <property type="entry name" value="RIBULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0054">Arabinose catabolism</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="12">
        <location>
            <begin position="6"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="13">
        <location>
            <begin position="283"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="639"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="639"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI64537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="821" mass="85498" checksum="25262F5C79299F9F" modified="2025-10-08" version="1">MISDHYVIGVDGGTESLRAAVFNSAGHIVGSHAAQYETQYPHPGWAEQRPEDWWDALGQAVQGAMDAARVSPEHVAAICLDTTCCTVVALGSDGEPLRPALLWMDMRSAAQAEKVAAAADVALEVNSGGSGPVSAEWMIPKALWLAECEPDVYGAAKTVCEYQDYLNLRLTGRRCASANNMSVRWHYSVRRGPPLSLLEKLGIPDLADKWPCSGPGGAVLAPGELVGGLTRAAADHLGLVEGTRVAQGGADAFIGMIGLGVIRPGQMALLTGSSHLQLGVVDSELHGRGFFGTYQDAVLPGCHVIEGGQTSTGSVIAWFRRTCCAPNTSYAELDREAAEVAPGCEGLVALDHFQGNRTPHTDARSRGALAGLTLKHGRGHMFRALLESVAAGTALILRTMSSAGYRPGSITVAGGAARSELWLQIHADMCGLPLRLTRCAEAPMLGCAILAAVAAGMYDSVPAAADVMVAVEREVVPNPDSVIAHQQPLARYAALYPALAHIFHGTYDKKYDRTPSNTETRQASCGPDGGGWRGAGADAGAILVAPSILAADFAHLGSAVSEAEAAGADWVHVDVFDGSWDRCPNFTIGPPVVSALRRATQLPLDCHLAVQDPGRYVDALVAAGASGITFHWEVFNHHQPQQQGEQTQQRYPHGNGTPNRTLSGSTGCCGEGGGAGGSSVAALAAKIRSAGVRVGVALAPDSDLPDELVAMAQRGELDMVLVMTVDPGFGGQTFQMQVLGKVRQLRASCPRLLIQVDGGVNVRTAVEAVAAGANVLVAGTFLYGHPEGLAAGVAQLRTAAVSANTATTTAAEMGGEGCFDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5L4</accession>
    <name>A0ABQ5S5L4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_008493</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004970">
        <property type="entry name" value="PRK06333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF336">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="10">
        <location>
            <begin position="37"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="455" mass="48390" checksum="64DEDAC4718F9588" modified="2025-10-08" version="1">MQLGQRVRPFSGASATRRPARAGSVAVRAAAATRDPKQRIVITGIGICSVHGNDPDAFYSKLLDGVSGVGPIDRFDASEFPTRFAAQIKNFDDEGMIDKKNARRYDDCLKYTMVSGKKALVMAGLEKERCSEGYSQVDPTRVGVLVGTGMGGLTVFQDGVSNLVQKGYKKISPFFIPYAITNMGGALLAIDQGFMGPNYSISTACATANYAFVAAANHIRNGDADVMIAGGSEAPIIPVGLGGFVACRALSTRNEEPVKASRPWDCDRDGFVMGEGAGVLVMESLEHATKRGAKIYGEYLGGAVTCDAHHMTDPRSDGLGVSTCIELALKDARIDREQVNYINAHATSTLVGDIAEVKAIKKVFTDTKHLKINGTKSMIGHCLGAAAGVEAIAALKAIETGWVHPTLNQHNLIEEVAGIDTVPNEKKQHHITAAISNSFGFGGHNSVVVFAPFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5X2</accession>
    <name>A0ABQ5S5X2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_007944</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014891">
        <property type="entry name" value="DWNN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF0">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08783">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01180">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51282">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DWNN" evidence="10">
        <location>
            <begin position="5"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="396"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="479"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="611"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="848"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1323"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="310"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="629"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="653"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="673"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="718"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="931"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1070"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1085"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1104"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1114"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1142"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1154"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1174"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1199"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1226"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1243"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1278"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1323"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1340"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1351"/>
            <end position="1388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI64626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1388" mass="148610" checksum="B4817C4815DABFC6" modified="2025-10-08" version="1">MTSSIRFKFRAELSYSNLPFEGHYITVGELKRLIAEKKGLGIDAANELQLTDATSKRDYDNDAEQVLKNSSVIVKRVAAAARPKTLRSGTASVAVAPAGTAAGPAAPVSGSVPTDLGPPAHVEDEFGEDPFLKAAVVQDEQQMLAFISQAAKGVGLESQLAMQAAAGRGRGRGRGFLGGRGRGGMMNRECLRCGIVGHFMNECPTQGDPAYDRRVKVPSGIPASKIHRNADGSLYLPDGELGELAANTQAFNKLAALMGRSKPLATEAAAPQGGQDGTSGSGQAVAASSGALQVTLSAPAWQQRGPAADAASLLKQQQEHQQQQQDLLQQQQLQQQQLLQQQQQQRQLQEQQLLQQQQLHQQQQQQLLLQQQLHPKQEQNMEAVSTVQLELGPVTLGLPSSAGGDPSKPYMQAPSNVPQPASGMEAVERSSVANRQPTPEGVQAAPKPLGPQLFDDDFDDGTAKQSMQQPVKLALGMEDVRRSSSPAPSRLTGSRALGPGGGPLREGGTAAAAVAAAAMAAASGRANGGVGGAHDRDRDHVMDPDEALLAPLRGVGCTRVELTEALPFLRDLLPGNMAMADLFRAFGSGRPLSRREFLDIQASAARIAADRAGRRGGSAAGMGAAAVAAERRDHHAGDRRGSGGGHGHGGRLQSDRHFHPDRERDRERERGGHSRSRSPFGRRSKRSRSGSGGHGGRGSRSRSWTRSRSPTSPKRRRGRDDRELDRGRYRERDREREAPQRERDRDRERDRERGGRERERDRDRERGRERDMGRQREREREGEGDRGARREATDGSPSVHLREPDQQQQEEQEARRQRSRSPVAAAGEGVVARAIRAEALDERAWQVKGVSGGGGGVWLRGTTGRGHGDAEGPSGAVACAGVASEPPSAASTDSERMPVGQAKPPPEETSLVMRPSTTRLRDDGGDDEESDGKGRRMEERKRRKASQRPRSATPERRESSRPTRMQPGPGAARQQSDHGEQSDSADGGENSDPSDRDDGAEDEDDPGVSGLGGRQLTSSQQRQPQPQPRPVPSGRGDRPSSDLSGSARSSEEFGGDDVEPTATAAVSTLDAGVAASSDSDSGDRRTHKKHKKHKKEKVKERKKESKKGHKHKEKEKEKGRTKESGRDRKGKPRQSGAAPATYDEDDPYEDEYDIGGRYDDSEDERPAPSRARHGSGAAAAAAPAIRTVPSGGNKAAKALSDDGRAHDHSRSRSPVRGTRGGGADEYGGRRGDGGRRGGGGGVGHDDDRQDTDGGARRGRIEWVLAKGRGGNRDTAAASGGGGGGGGGKDAGDGSAAAATRPQFRQGFAARMFNQTLGLALQEQRRVAAREDGRKEALGRSDGGNVGGGGSDRWRDRDGGKDRGGRDRDKDRSPNRDSEDERPRRRYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5X4</accession>
    <name>A0ABQ5S5X4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 92 protein</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_008289</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 92 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF69">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="332" mass="38189" checksum="D23E8D69C2BA8EE2" modified="2025-10-08" version="1">MKNSFRWLMACLYIAFFSILVFPRVVTSYRGAYASICTNVKDEGTYLLEFILYHHWIGFGKIYVMDYNSSRPLFNLRMLMPFIRSGIVEYHYDADPKHHSHERGRLFVEYSQGTAFQRCIDFAHRDHVWMAFIDADEYIVLHDYEGGRPNITDFLRGYEKYPGLAVNMRVFGSSGHIRRPEAGTLEAYTKCTPSSAYENRHIKTIGRLSQVLSIGGNPHEFRYFNAAHKAVNERGIPIQGPMSLPVSWDKIVLHHYVTRSLSEFVFKMQRGPGGSQNKRNMDWFKAYDGNATEICTEGVPLWRVCCASKYASMLAALRRSTKQGSARVVRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5Y4</accession>
    <name>A0ABQ5S5Y4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_008145</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="372"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="660"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="773"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1028"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="424"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="777"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1051"/>
            <end position="1067"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1312" mass="137498" checksum="DB353B9618F80812" modified="2025-10-08" version="1">MEVFQHCCWLGHGSYGDVQLCEVSSRTMEMLLGAEHQQVDKQAPAPILIALKTWKNAHKEGKVMQIALREVQLLKAVSHPNIIKLQSAFRTQSGHVCMVFEYGGKSTHQLLETRFPGGFPQPKLQRLTFQIVQALRYLHSRKIVHRDVKPANILVDSEGVLRLCDFGFARYLSGSTNPEAVLLSTTRDGNDPVGDGSGDSGLWTPYVITRWYRAPEVLLGMPYGAQADVWSLGCTLAELAIGEPLLPGTSSVDQIGLIANLLGPLPNHMTSRVTFPPTDKLRSGAALPAGGKVAPAAVEALRQRLGPDHGAEFLDLITACLKTDPRGRPTMEQLLGMPYLLDAARLFDGSSLADTYSAVSLAGPVNAARKLAAPSQSFPPPQASGTDGSQSSKAQPVQPGQCRFHRCHPHPPPHQQHIHHQQQRCRVNLQQQQQQQQQQDSRARDMFRCSRGLQHSTSMNSSVDAGSTLTSASCSVMSTTPVMRSVTSVTDSSYSTIPLGSCTCCSICSGAAGISSDVVCTCHVESSNFVSGSASNAQPLLSPAVAAAATAAAVRPSSCRAHIGSSMYAAVARNTEPDPPEGLRGATEEAVAHPPLVGIGMGGSDGTCWRGTAMVHGSGKSCVNQACMGQVDADDPTAAMASSLSDVTIAHPQSLSVTAAAARPGPPTVHLDSQPAPPHPSGLSREAAIRAMTAAAAFATNESAAASTVAAAMAPSSRALSLYFHHHHHQQQQQQQQQCFAVVMRAALAGAHGDLGGEPVPCVLNEMLVRDNGGSGCSTRSRSSSSSRCSSSSGDSEQICASRTDTTGPEFAAAGAAATAAASDSFDVSGDGIETGARQAWRNPAVLRSQDDYDCVFDDGGDNGGRGGYTGGGRGTADHPMQMLTAHWLDNYYHDCSPVATSAAATVVAGLKAMATAAACGASSHTTVSFNWHNAESPCSGVLGRIQGNQPLNHTMALGSDAWPMPRPTPPPPKQQAVGQVLEMQPLAAQVQPPQPLKLRPEQQLLRRRRHLCGYVVAPAAAGAIVGASQAHPPPQRPAADISCSDDRYVDGSWSSPGDAGSGSDSDSGCRPRDLTWTSFALRRGYASAPGAITTITAAPNADACVALPCRMPNWTRARWAVTTTAGIRPQSGEVLRDAIVASGLACGPVWPPYGSPRDSERSTCEESMGMRRCDDAGDSEARSADVPIAATAAVVAAAAAAAAAQGPAGGAAQMHVAAGSVTVAAECINSESFSLDSTTGARSCCASIGEQRGHVTAVAEAGNTASAATCRDVSSRGNKSSHRFKRWLRKGRNWLRHLLQGSFPTRESLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6S9</accession>
    <name>A0ABQ5S6S9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13556" checksum="3AE79E20E6967407" modified="2025-10-08" version="1" fragment="single">MGKDRLDEPLDLHNYSTWKLRFQLLCAELGYSRALLQAPIAEADVIISDKVKSVMAKNVKNHHLETINRAANAKAAWDALAATFASTCNTRKVALRQELSDFKMANEDGRARQAVWQGKTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S738</accession>
    <name>A0ABQ5S738_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="43"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="345" mass="35916" checksum="0BAC38E62D9697BD" modified="2025-10-08" version="1">MSIAQQKVLRACSSRSGRCTGLRVQATAEPVTVAPPARVVVAGNGAYSIKGTSRKVNEDRYDVKIASPGEAGEPFAWAGVYDGHGGYAVADWLKSKLFGVIETEWSSGYKPEFSITEAYLAADRKLLSVGAGFMGMGERGVGGSKCGATAANALLFTAQDGASTTLLTANVGDARIVLIRGGQPVQLTEDHVPDREAERVRIERMNPNPKLPLVRYMGGTWRVGGLLALSRAFGDAYLKGSLQFEGVAAGSDGYSSGFGVIAEPYTTLTPLTAEDSWLVVASDGLFAEEERGGGGGLDNQGLAEVLATVPPGADLNKVAEALATAAVKVGSTDDVTLVVMRLALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S752</accession>
    <name>A0ABQ5S752_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_009348</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17729">
        <property type="entry name" value="BRCT_CTDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039189">
        <property type="entry name" value="Fcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081">
        <property type="entry name" value="RNA POLYMERASE II CTD PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081:SF36">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="10">
        <location>
            <begin position="965"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="9">
        <location>
            <begin position="1246"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="360"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="564"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="377"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="393"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="440"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="537"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="564"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="661"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="752"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="772"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="790"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="891"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1341" mass="139125" checksum="DBB604F4E2B4448D" modified="2025-10-08" version="1">MPGPPLEEGELEDGEVHEDKHADLEESGEASCCDGEDDAGNLAADHNQHDQPPPLRATSGQLGTNASPLPPPPQVLQPPAGANVPAPSRTGGPTSDGGGTHPPHSHQPGGPKGRLRTSGHEANGNAGRSRFRAPEGQQTGPPDSQTQLQLPPLSPSRAPQPSPHAQHPHQQLHKHPPHPYGSGPGSATGLHSPPGSVPGCIGGAVSGSGPAAALGREDREVDELIGLLRDLTRGCSFQEVMRSLESVCRDLKQAIKKLDKLFRRIKRRKGDSIEDYPDCFQFSRRILEGLTSINKFSATSAFHAAPPSSAEAARALFKAAMNYRHDILNGALKAELEDMVRNSKAFKCVLENRHRATTVPQAAAAVAPATTTPPAPSATAAATASTPVGAAQRPQTTNGGDSSTANEVTEPQAGVHGSGSSTEVAACTDGEKQEQRRSRSPPPPLPPPPPSALGRAAPEAEPMETCEDGPASATTAPPPPADAPSTFTADGELDVAPGPPPDDDQQQLLMMQRLASSNSAGGGGGGGRSTVARPGSNAGGGGGGGATAGGFKLKIVVASTAGDLDAAAAAAAEVEASGATGGAAVAGSAGPRSFAGGGQDGGGGSARARSPTPSGVNSDHHRAAKRSRVSAEPEGVPPESTAAAGNSPLPGSQPAGEFCWQPRPQQQQLAHQEQQHQQQSGRPVPVIMGCSMLPPPPESPVSEAGADSDGTRSEQQHQGAAALHRAPGAGVTDIHQQQASQQPKLEPQEGQPPGAVSSSAAAIAAKIKPAGGSEGPGQQGGQTHGSGGSHSDRERERERERGPREKEDKERDRDRDRDRDREREREKEKDSKDRDRGRDRDRDRDRSHRSRQDKDKDSKERDGRDREREREKDRDHRSSSHRHRDRDSALPLPPPPPPPSDPSSQGTKPEPYEGPLAGTGPMGRSTVLPASSDDGTGALTAGAGANGGGGGGSAATAAAAALSLLSRGKLVLVVDLDGVMADSCWDDQLDTAAAAALSRRAAMEAGMPDDRRELYRLPLEGSCLWLKLRPGVRAFLSRAHERFELWAHSRQGRPYADAVVELLDPSLALFGSRVVAQGELARRLLTALDARAPIATILDTPNACWMGDQLAPGLLPLPPYSYFTYRPACIPAADATAGVGGKAAPALAPATGMVGRSLLEVDRDECAERGMLAAALPLLDALYNLVMTSYGSGPSTAAATASTVANTASSCAGSAVAVKPSPDGAPAQSLMAGLEPWDVRAVLREQRQRVLAGAHITFSRVFGSGGGTGTGPQHPLWRLAEACGATVSLRCDEATTHVVALSAGTEKAIWAAQHGRFVVYPSWLEASCFLGRKVDESLFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7X0</accession>
    <name>A0ABQ5S7X0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="477"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="506" mass="51920" checksum="47550E8E1610F405" modified="2025-10-08" version="1">MVWASHEPVDDSTPAIDSVRAKPLKKRCSSRAGLSRYYSSKSQSFSSLNLVQCTPFGSSAMALAKRPASFDLRRGGSPRPRLSHVTAAFVHSDKEINMSIPDEDDGGKDSEQEAPDRCPCNDTIFSDPTEAFMSPEGSPNTSSLQMYDSCGLSDHDIGSYQQQRQYQLTSEYLTHLSTLLPIPLPSPSSTNGGGAAAAGGGGSTRSSGLSSVLSSGCCGKTLSTGSSNSSSSSVGGSYCDSSMASTARLWQLASAHIATSYSAGLDRPDCHVTGGSSGVFSRAWPQYPGPGPISSLEAVTQTLLQSCGDDMKIMGAGSGCGRTVNISGTGASYPLASQTTAFGASSSAVGSGILPRNCRTSSGYLYPVLHGSGSAAMSAVTLGSSAPTPMVTTPTSMVPATDASHANGSGSSANGAVPALGRKLAMGGITHRRLRAAQAAGLVAVHSARHAAAGLWGSTEDMIASLTLSDKQLLQQQQQHQPQQHLLGSRPPAPSSVALVGDGTCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S819</accession>
    <name>A0ABQ5S819_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="219" mass="21320" checksum="4CAB640D5DF09D67" modified="2025-10-08" version="1" fragment="single">MMHPLDLAAAHLAPDDWLLSGPKGRLHWAEGWVAAHILDALRAQKPLLLIINGGGPNVVPGTVGTAATSPIAAYTRRLLEQTFTALERADAAGLAVAGTLVSAPTLDWVPMPMTTADTTTVAAPGAAAAAGATSGGSGGDGTQAAGGSPPRRDSTVVKTPGRSSNSDGAGRRADGAGGGGGGGDGGGDGDSEVGVEIVSGGADGDAMKIKDGDAPKNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S871</accession>
    <name>A0ABQ5S871_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="123" mass="13631" checksum="3ED1C99C31CD477B" modified="2026-01-28" version="1">MADPEAVGRAFLDYFYGLFATNRAGLASLYQDSSLLTFEGAKFQGQQNIIQKLSTMPFQKVAVQRDTVDIQPSISGGILIFVTGKLMPEGEVQPLKFSQTFHLMPTPSNSFVVTNDLFRLNYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8H0</accession>
    <name>A0ABQ5S8H0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009832</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Assembles a suppression complex (suppresome) by tethering SIRT1 and MDM2 to regulate composite modifications of p53/TP53. Confers both deacetylation-mediated functional inactivation, by SIRT1, and ubiquitination-dependent degradation, by MDM2, of p53/TP53, promoting a proliferative and cell survival behaviors. May play a role in the regulation of spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052472">
        <property type="entry name" value="MORN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="495"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="512"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="537" mass="57120" checksum="1754A613499A452B" modified="2025-10-08" version="1">MPYDVQPRTNTGTGGAPTGKGQAERPQMDHPSTKYREIEMPNPVIMHRANSPPRPTLPRRSEPRSWKATARERIPLTSSLTGPGRAGKQGTLLGVSGGRYEGEVLAGRPHGRGQYFVHKEGSSSKEWVLQYDGDWIQGRREGNGTRYYPSGEVYSGDWVANIRHGTGRYEYANGDMYVGQWADDKRSGAGTLYMASGDIFIGNFMADRREGMGTLYMMTRQKKYLAEYVRDSPRCGTVLEIDDNDLAPLKGHLASLATARKLDAAAAREAISRLPPLELLQPSKVLAEQVVSVRRDRQQGGNKALRAVQAASGNLSERDIEMLRHSYTLMAAGDGPEVGLLPHQLRELVVMAGLDPAAAATRSLVEELMKRRSANTGRISFDSFMLVVTHFQDEAVAAEAAAAVENAAAQAAATLSATAGMRGIAAGTGMAHMLAGTAAVAAEAAAAEAQAEAEAARLAAVAEGDEGAEGDYGEGVYDDEGDGEEHAAVRAAVAAAAMSQVPAPNDGHDEEQFLPEEGAEEGEGEGEGEGEGVPTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8L0</accession>
    <name>A0ABQ5S8L0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009379</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI65764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="254" mass="27190" checksum="D39FFF450A43134E" modified="2025-10-08" version="1">MAFVLATSRKALQVACKATGKKPAPKPAAPKSSGIEWYGPGRAKWLGPYSENATPAYLTGEFPGDYGWDTAGLSADPETFKRYRELELIHARWAMLGALGCVTPELLSKNGVQFGEAVWFKAGAQIFQEGGLDYLGNPSLVHAQNIVATLAFQVILMGLVEGYRVNGGPAGEGLDPLYPGEAFDPLGLADDPDTFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPIENLNDHLANPSVNNAFAFATKFTPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8V8</accession>
    <name>A0ABQ5S8V8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="106" mass="11591" checksum="1A27B7D32966ECA9" modified="2025-10-08" version="1" fragment="single">LAWGLSLVGACSPRTWSRLMELLGVAVDAQGGLEELPAEALTQVYQAYLNVRLDHPSVTQVVGVPGLLQRGAQVWRTNNARGVQVSAFQKQVSHTLLRLGLRNKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S953</accession>
    <name>A0ABQ5S953_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_010092</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="148"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="88"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="269"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="415" mass="45464" checksum="900F9A52EF013946" modified="2025-10-08" version="1">MDPFIECMLTAVFKDPACSVIRPPVAMLTFSRYTEAPGARPASKTIELWSRYHCGSSSSGSDSGCARSNEPSEFCEQRHPLLFPNQMNRGLQLSSPRPAGQPCTPTPENKAAAEAHAETHAASCRTSPAGCLHYHQNIPPEHRVLIVGWMRQVCEALGLHASTLFMATSLLDRFMAASQVPTPEGILQLLALASMSVAVKFNEVLQVRPSIWLGLAVNPTGGHLYQPCDLQRCEFTLLQTIDWQLHEPNTFTFLEHFLVSQRKPLRGQTQVDSSCSDDSWGASRRAHSSPGPRPARSASVAPELHENLHEDSPNTSEFCESAVATVAASLAELSLMYDTFLSHEYCTIAQACVMLAQRLVDQEGMALRGVVETREPPLRPPEPGLAACIEALELCCSPGERHFAMAAEEGRTRSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9B6</accession>
    <name>A0ABQ5S9B6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="757"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1352"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1461"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1687"/>
            <end position="1730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1976"/>
            <end position="2080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2096"/>
            <end position="2156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2386"/>
            <end position="2411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="760"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="784"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1695"/>
            <end position="1705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2000"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2041"/>
            <end position="2062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2063"/>
            <end position="2074"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2411" mass="245943" checksum="528747C75F69DF88" modified="2025-10-08" version="1">MGRRLDWLPPRPSDDNTELDVPFVQETPDVVRVLILQLARGAWSTLARLLQLACLTLDYKPLLSHEMAHAGATGVVLDILCQAPRYHPSWKALSTAGAGLLGALVRCEDDGTEAAQPQALRMLLVMLESDLTCGPARQAVATVLHCIVAQRTVHRDKLRAAGALPVLLRMLRRLDVLCDLVAVQELLWTLVYLTDEAALSLPSNGFSPGAERLQPLIAERLVPLLFLFGMPWHAQHEHLSALTTRALHVHVAGYDAGKDTLRAAGMLSVLVDQVRRHSAPYIAAAAGASVAPSGSRSEHAKQTLGDGKAAAEAAPPTTTHRGQTPANRGSFAVGTVRPASHMDCDVHADSSALHGAKGWGENGPYACGAVGALGPWTPWQRLLAVLLISGLVPGVIPRPRRRMGNRCGSTVAPLVGADDGGADSGVEGTGGSAKDSLTGRHRDEGAKETAPESEEGEDPSTHSTMRSEDSAVGVRVGSGASYAGKANSMALAPGCNFSSEIQPVAGTYDGVEAMARSSRGAAAAVAVAGVSEQAEEEEDDVFGSNISEISVASPSGGSVLEVATAGHGDGGDLQHGSSSTDAAATAVADGARVVCYGLMLPLLLDAGEAAAGGAGAAAASVGGALLRRRLCPLRESLATLKTLMCDCEANQIAVSEQGLAALLVQLVAHGDMGLAWQAAEAAVFLSGGATGGALVAAGAVEEMAYFTEESAEMLHSLTLRKQIQVQNSTLAHGDHHHHHHHHHQLVVVEHQRHNYHHTRAAGAAGQQPAHHPQRNGQHRKTSFGAGSSAGGAAAAPPALNDVHVRPNRTSTAHCCSSRPQRVYLVGRVPLDFTTSSSCFYGSHDAMSYCDYCAPSAALKVLLRLMTATQTSNSQMPTTPSEAAAVAAAAAVPQEDAVCRRFAAAGGMYGVLLLLHHEAAGVHHSKLAASLLTLLDLLLMRAPGSQDELRMAGGLGVLTTLLGSLMRPGVMDVFQLRVATCHTLRLALQGNAANKLAAREHGLLPLLVSLLAGLTQQRKARSSEVVVDDSGPLTSAVLEALTAATQDSPGTQATLVGLGTLYPLSDLLRDEKLDTEVRTVAVRTLTAVVNKYQAGQAGALVLGVVPPTVAWLEQCCMATSNAVGTPRGGGGGAGAATSVVVQLVEPAAALTALLGALLHGDSSYAKPAVVSAGALGVLLQLLRDPVLVAAVPAAAAGGGSAGRSADGGVTGQAAAVLTKLVDGDTDLQNELVAQGGLTAAADVVRDGGAAAPQALMLLSAMLRSNTFVKNTARQAGVPATLVAVVRRCGLIQSVNAAAAASALAELAVGNHANQEAVAVQGGLEVAVDLLRDTFAALPLKILTGGGGSIPAATAAAAAESDDSEGRAEEQTGTSGGSLLQGAGPRDAAGGTYTSMPYTAAEAAEDVTVAASLSRLLCALLGLVCACVELNGGNKAHIRELGGVTFLGELLLVMAEALTAPPRLGWPPYTSQGTQDSRQGKHGFAQGDGQLQSRAALPPPSLPGPVEAARLRDAAAASRTAVLPAVAAACSALVAVTRESSANLSRLEEQPGLSVALFKLLVATDAPTSLAAALAIEWLVIRGRTILDAPSREAFELQLVDVLSLALYVPYAPVPLMLPPSPKSDGAASGSGTVIGNSGIWQQQQQPWYVVVRALMPPNGLAAVAVRDAATVLAAVADTADTLDVQRRQQLSVQQPQPQPQPQPQPQQPAIKGAAGVKSTLTTTSRGSLPPLETAPIDNVALDHKTGLMVSNLLAGTSSFPGGHGGASASGGSATALGSQAWWAELAGSAAAAFQRVANGALQGVSATPDGGGGAAAAASSTAAGTGPTVRAVAAAPVAAAGMHGTATMASTAAARERRRQVTWMLFLLAVKSTENRARMRWLVRASLTPLAQQLANAEQCRAQVEAAAAGPYQPMDVSGSSRSWSETMFGSGPAAAAAGPAMERTSSAALESASLLRGRLPISTAAIEELGDNGVVQRSSSPFGGSHVAATHGAAHPPPSTASAESSQVAPLSLLHEPNPAGRVGLSTHQHSHRVPPSPSHQILQQQVQYAQQQHNSYVQQRYDQSRQRQSHELQQEQQQEQQQQQQQQQQQQQQQQQQQQPQQQRPNVSQQLATLPPQSRPPSRPTSSSSACGVQHVVPTPPPATTGTAHHRTSSTGVALAAAGMLHGRPYGTTAAAAAVPATASSSSSMPATPREAYLKASADSPAAAVLRFNRLNRTASGGQVVFPPGASGSAAGGTAAGSASNTPRTSLTGAVAAAFSSPASYLGVAAAGRGPRGAAGPSPIRAGRMGHAQIDVAALSAAASPAGSGSAFAVTGGMATPLAVPGQRGLGHQVSAGGSSIMSDDDELTMIQQEVALLANTTSSLAWNTGLQGSAAGISPGVGGYRSTGTGGNSPAASPMGWGPGGAARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9G2</accession>
    <name>A0ABQ5S9G2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial ribonuclease P catalytic subunit</fullName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial ribonuclease P protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">VaNZ11_009899</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18718">
        <property type="entry name" value="PIN_PRORP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033495">
        <property type="entry name" value="MRPP3_PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033443">
        <property type="entry name" value="PROP1-like_PPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031595">
        <property type="entry name" value="PRORP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13547:SF1">
        <property type="entry name" value="MITOCHONDRIAL RIBONUCLEASE P CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17177">
        <property type="entry name" value="PPR_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16953">
        <property type="entry name" value="PRORP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PROP1-like PPR" evidence="19">
        <location>
            <begin position="187"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="PRORP" evidence="18">
        <location>
            <begin position="375"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="132"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF17177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="GLI66200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="631" mass="69853" checksum="3BAFE5C35F126ABB" modified="2025-10-08" version="1">MEKGVEESQIAPAAKRQRTDDGLATNRAAGSGVGAKMQKNGAPADQHNRKDAGRRKKLDMRIPMLRHSVKRNNLKFAMNLFREVMEEDVKMDTSLLNSLMYLACGAEQWERYARGLPLLTAAEVAAAAAAEATGMETSSGDAEAGGEEGQRQQRPGTQQQNKHRPGKSGVPEVPEPEGPPPTREELVAAVDELWSHMGKAGLQPDQGTYLALARREALQGDPNGALRWVKECVKKGKPVQLRLFHPAQVGYCLRSDVSKVHEIDTMIASHNLDNTEYEFARLLEAIAAAGSYAQLRAVLMRMQTDLNELAPSTADYIATFFKGNPAAQQAFVPVDQGGCPGVVQRPAGAGDAAEAPAGAPVGSWQWEVVRDAAVTEAGWCKVANGSVKVVDLEDKDWEAFATAIAQLARSNMGHRAADFDAYVQWYERNGPYDILVDAANVAFYAQNYEGGGFNWRQVHHMYELLRQRFPEKKILVMAHRKRILDEAAQAPRVSEFLGLLRRNKSFYATPPGANDDWFWLYACVRAKHNGLLVSNDELRDHIFSLLRPKHFLKWKERHIAHYSLASYNANVTASVQLGELFKLQVPRPYTACVQQLAESGAWMVPINNGTWLCVRPVHEAQQVTVAVRPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9Q5</accession>
    <name>A0ABQ5S9Q5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATPase synthesis protein 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="58767" checksum="072FDD43F5E7A6D3" modified="2025-10-08" version="1">MFVAVSSLLRQCRHGSVRQQLTGAALRPASERIQIDLLAPDVFDQASLSARASSSAAASTSSPLYQSSWPSGYTIKCFGFTVRHANTASGGHSARRRELLRDAAQKVSPGSTWLLAYSHNIDPQQLTYRLGKRSTLHALEEGWELYQQKFDTVAMAALLRRVRVAQLYDTDFDTSAAQRLLNALEPRLRSVTLRFGKLRDTTAYLHALAKLQSPPPQAPAPGIAGLPESSNDDSPAAGPSGNDRLLLAQPADLILDLAMWATRDRVQMLNTSPRRLSTLLWALLRTVPSDLYSSEKLQLVLDRVALCSIRQLPNFSAQDMRVFATVYATFGPRGAPTAVSPSDPDGSWSRNGRSWLGNVSSWPKPVKILKAEGRNPGDDSGSAEPKAGVASHSSGSSGEEKVVQRRKRNALVLQAMANELVRRVGDVTVTQPQTRDLALVAHAWALQGAPTPHMPLLVKAADAAAAASHSLLPMELVLLVESLAKFGVRHSLLMESAQQRARGWAGTDAVSGTEGTGVSDVGKGQLQQLYWRLAEALSSLGVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAI5</accession>
    <name>A0ABQ5SAI5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06467">
        <property type="entry name" value="p23_NUDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.740">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037898">
        <property type="entry name" value="NudC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12356">
        <property type="entry name" value="NUCLEAR MOVEMENT PROTEIN NUDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12356:SF18">
        <property type="entry name" value="NUDC DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="1">
        <location>
            <begin position="16"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="147" mass="16547" checksum="EB41FF3DA0E197F9" modified="2025-10-08" version="1" fragment="single">MADRLAPSTRHQYKHNGQVVYEWDQTINEVNIYVNVPPGVSGKLLYVNITTQHLRFGMNPNPPYIDSDLAAAVKVSESFWTLEDGVLHVTLTKLEEGEVWGSAIAGHSLDPITQQADQQRLMLERFQKEHPGFDFSQATFNGQVPNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB39</accession>
    <name>A0ABQ5SB39_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011085</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI66989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF185">
        <property type="entry name" value="WEE1-LIKE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="204"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="97"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="477"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="502" mass="55547" checksum="B51FCEF430204272" modified="2025-10-08" version="1">MASASMKPSSANLFNYYNKQKLAQQCSESTNSLQLSMRHMNIQDAAQTLSQEPRPHMRSIRPRALDLSSQPSQEMQYTPDYLTPQDQMFRMDYEPSSMQLDDEVSRSPMPSPRAQKRPRPLKVLPSGGDEECSSQSLSQSRSAGEPSTCSKALSGRPPIPKLREIQPPMPRNPYLVRADDDSHVFQASQSCLNRGNYSRFLWDYHQEAELGHGNFSKVYRAVHRLTGVPFAIKTNRSPITTLQARNMWLNEMQALAAVQHHPHIVGLYDAWFEPDVRGDAEQAYLKLELCGDSLGGVFKRRVQLKEQEVLDMLRQIASALKRIHDLGMVHLDIKPDNIYIALESSQTNNNSGAAACSSSGICTGNVYKLGDFGLAIVQGGQRAGTSEGDSKYLAPEALKSRDFLNSGLADRLDIFALGASAYELLRGSELPKNGQVYHDIRAGKIFLPGSNQRLVGLLKKMMAPDPAQRPTAEQLLRSSLLNPAGSAGGQLQPQQSPLPLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB92</accession>
    <name>A0ABQ5SB92_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Testis-expressed sequence 9 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000039">
        <property type="protein sequence ID" value="GLI66733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23313:SF0">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23313">
        <property type="entry name" value="TSEC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="41534" checksum="FE382643D194EE42" modified="2025-10-08" version="1">MSNFSYGAAIQQEAYGASGNEDNVLQRSSDEDLDDMVQRNIRDVLRQASVDKDGDAYGEMSQRQHDMGCKESTHQSQVDDRNLQILGHGGGPEDYGYAAAHGTRQAGNRHPGGEGRKSLTGSASGCKLRSQDLPGGSSATATVPHTAARRSGDLPNANIGAVSASLLDVPGQPEVSLRLHKVRIKTLEEDLSRANRLLVDREKQLTEALRELKELRGQAALWAREKKALEGQLDRAKKQAAEAESALRSTESQVRELAKSDSRAERERRASEAEVRARDVRLQRALEEVERFKQLLSDVRYQEREAKGSAQQDTARLLAENRKLERQRAELISAFKKQLKLIEVLKRQKVHLEAARALQFSEEEFMKTLEMGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCI3</accession>
    <name>A0ABQ5SCI3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Intraflagellar transport protein 88</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44117">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 88 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44117:SF1">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 88 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="259"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="472"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="506"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="540"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="574"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="756"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="776" mass="86079" checksum="581A751753A39D04" modified="2025-10-08" version="1">MSYAAAEDDDLYGGYDEQSNPLAGSGGAAFKALGSDALPPGTAMMGPPGTAMKSFVPGTAMRGGTAMQDPSMARPMTSNRGAGFTSAPNKKFDPLNRSMGSTLGSTGGGTILATRKGDVTPEEQARSLEKMVHELLEKCAVDCVKGDMNSALEHANEARKNERKLCRFRQQHNMAEQINLELMYAVEFNLAHMHHMNKNYNDALNLYQAIVRNKDYPQSGWLRVNMGNIYLEQKKYPTAIKMYRMALDQIAATAKEVRFKIMRNIGLAFVRLGQYPDALQSFATVMDNVPDHQTGYNLVMCNYALSDREGMKTAFQKLLKVSPPHEMDDDEDDALNDEDEMQAVVADDGLKEELRKRNSAITRLIVKAAQLISEKVDRANGFEGGFQWCCEQLREAGYTKLANEVELAKATRFMGQKQFDKAVGVFKEFEKKEPRVKARAATNLAFLYYLEGETDQADKYSEMALKNDRYNARAYVNKGCVLVERGELEGARSLFNEAAGIDPYCVEAIYNLGLVSQRLNELPYALAAFKKLNHIVPDNVEVIHQIATTYDMMGDFKNAVKWFEMLTSLVPNDPGVLARLGAIHARFDDEAKALHYYQESHRVYPVNMDVISWLGAYHVKSEVYEKAMPFFDLASKIQPQEVKWALMVASCYRRTNNLPGALAKYKQIHAAHPENVECLRYLVHLCSELGRRAEAGEYMNKLKKAEKAAIPDAATVAAAAAPAGAPVGGMGIDDDLGSSAVSAQNRGKKMLVKEHMGGKEADDWGNEQLGDDLLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCI5</accession>
    <name>A0ABQ5SCI5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_011539</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex with RFC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the activator 1 small subunits family. CTF18 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053016">
        <property type="entry name" value="CTF18-RFC_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46765">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46765:SF1">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="729"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="22"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="403"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="593"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="825"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1255"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1409"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1472"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="842"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1262"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1285"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1303"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1416"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1477"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1494"/>
            <end position="1508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI67361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1542" mass="162135" checksum="7E04A35A30FD30FB" modified="2025-10-08" version="1">MEAKLPDFCSLGTEDLLGLALDPGQEYEAEDESQDLVQPGVGASTGASQPGHKTAPSVEPNGVPDPARTFIYAGLPGHVSASDAIMTTQELDSELRHLPLDSEPQPGSEPQPEPFSSEPVPHVAVTQSGVSAGSGHSFPELVVLPDTLDTAALDAELRELPLETQDPNVNTDGGTGVQPMGGGQLQVEVEMATVDALEPQSFNTCGIDVGIDGVYTDSQELNDELRALPIESVVEDDMQPPPVVGAIQGGVDLSAPEDALKETAPAAASAQYEAHDLLDPDTVPQREVHDLALRQHQPRHAQANRLEPSSWDAFDDFGGAGSFEDDLEDYLAAQAADAQQVAMQAPKPRQLEPPDLDGLDDLIAELECGGRAPSARPSTSKVIARALDNSSMHGTIGTGSAGVDAAGPSCSSAARTWEPPARSQETVHQCAARQAKRPRIESRGRPTMEELFGSDEEDEDAVMVASLEAVPGLRCPADAAGGDVRDCGTVEEDEAAHPSRPSYFPPQRLASRISDGVALPVTAASGERVYCRVAETTGSADRGALIRAVVQQAGGDGGGARARRRGRGCFLEMPLVDMLRELDDRQLRAAVAASEREERRRQQQQPQRALPTEEKIGFADMAQDIAGGAAAEGTAAALCGRRDRRTSGAAVQDPFAAAVGLDEESMLWVDKYAPRHFMSLLSDERTNRQVALWVKDWDECVFGRMGTAGGATGTGKVSAARRKVDSRPQHKVLLIGGPPGLGKTTLAHVVARHCGYHPYEINASDDRTASTLLTRIQDAVQMTAVLGGGRPNCVIVDEVDGATGGTETGGAVAALLKLIRAGEGATAGGGAGGKAAVPGTDADGDGSDGENEDIGEGGRKAPGAKKRVGGRQGSQRRPLCRPIICICNDLYAPALRPLRDVARVFHFSAPASERLTARLAHICKAERLEADPAALRLLVERTDRDVRACLNTLQFLARRGAMAAAVSGPRRTIDVKDIETLNIASKDTTQSAMDMWTLLLSTGSSGRKRGGGGYLSGAGTAPSQLREVCNRLQDFGDYDLVLSGLHENLPRVRFMDINLNRTAEVEEAMGLADVLLRSCRRTGDFSSLQFVPPLLAKVRALAAQPERPRQLAWPRMGAEAARRTSAAAQLVRSWASSRGTDPGVIASHGITVMMLELAPALRALASQPPIRAVAPNMMTADEQATLRRTADIMLHYGLRYCFELPTPPGLQPLQLPEVVAPASNNAIILGLTAEVQKNIVAATGGDKDTAHGFQRQIPQPYGGRGPSGGFQAGRGGRGQYNSYGQGRGPGGNAGYRGSPDTVGHGAAAGAPPLSAPMATGKAPANPTRHVPLVPAIDTLYVFTAFRGKTSAGATTTVPHVVRQMISQQTEMEAIRRAEAARMAASTSSGSSRLMDKVDSPGAEPEIVARTQGPLQPVSPPQPLPPQRQMAGRMPSPTDCKKSAAGGGGCMGNKRKGTWMDAFKQQASAKARGNVGEGASAGATARQAARPASGVTGGGSSAGSPQGGVMIGGQGRLPFPVLYRFNEGYTNAVKRPLLMRELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCM2</accession>
    <name>A0ABQ5SCM2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011572</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="11816" checksum="230840C31ECE15F7" modified="2025-10-08" version="1" fragment="single">SLSASFVSRQLVNACPRLQHLALSCNCSAGADGETGVMAALAAAPLAATLVSLELHCGGAFQPELHHLDGLGAALTSLRVYGSRTLDDEALAILPRHTPALVQLMLTEQQSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD39</accession>
    <name>A0ABQ5SD39_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011796</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22271">
        <property type="entry name" value="DPBB_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692:SF5">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Expansin-like EG45" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="283" mass="29723" checksum="380DECA565E974E3" modified="2025-10-08" version="1" fragment="single">ASCGTCLEVKCASSNPQVCTTATSVTVVITDECPSCAAHELNLHAFAFQQIAHLEYGNTAIQYRQVECSPMDNITVFVDGFRVVQGGWLRLSLKSVASDGAVVRVELGRSMNAGVDSNTTETAATSGNESMATVPSGQWKAANPSTSATDSIPNWKLMTNTYGAEWEVSGLPSPPYDLRLTDRFDRQIILQNAVTKAGALGEFPGRAQFPGLDNITISNKIVASIKVSAAMASVNTQTSSEAAMQMAAPVPQGYNPAATPVKAASHSALPVRAAPVLKSSIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDG2</accession>
    <name>A0ABQ5SDG2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011691</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05382">
        <property type="entry name" value="CAP_GAPR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034113">
        <property type="entry name" value="SCP_GAPR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="524"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="699" mass="76133" checksum="88E7C180EB398B97" modified="2025-10-08" version="1" fragment="single">CYSQDMTPQLCDMAHRAATAIMSVTARVVPLIACLLLSRLAQRQGPQPFDAAGQAWRVKSILLSTITITVTKPAITLAMPSVRYRPPSPRTPASAKPRGGILLPLLRPIPFRFSSPRPPQPKPPSPHTATPPSAQPPRVLNTPPVGQLLRNKLPPSSQRPLRPPWSPSPPQPARIPRLPPVPRRPPSPYLSPPLRSSTPFWPRSTPLWTPSPRWPPPPQPWPPPPPPPPPPTPSPRHPPPPRRRPPPPRPPLQRRPPPPLSPPMLHPQISLRPPYPRSPPTPPPTSIPPPRRPPQQPQPIPRRPPPPQPLPPPPQKLPPKAQPLPRVPSPAPPQPLLQPPSPRSLPSLPRLQPAPRMPPRPRLAPSPLPRPPPMVQPSPRPGPAPPLQLSQPPLQVLQGKPPPALQLRPSPPSPPSPPQPSLHPTLRPPTQPLPPSQVLVSPTSPRPLRSFPPRPSSSRAEPPPPPPQPQELSAQSYPEQMATDAKTSPLSELPAPPSPLPAFPPPASREDLSPDARQAGGSCSDAATALNATNSYRTRHEVPALAWDPALSAGSEAFAWELISRGGCQTGHSSDFTYGENVYAEKQYPRQNEDTCRKAVDDWYNEVWSFNFNTRRMFTDNAGKGMGHFTQLVWRSTTSVGCGTALADYPVVFPSGIIRPGSCKVVVCRYLPPGNIPMDDAFAANVFPPKPLGSPPWRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDJ4</accession>
    <name>A0ABQ5SDJ4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011872</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="244" mass="25216" checksum="A2F87FD17940A205" modified="2025-10-08" version="1" fragment="single">ALTDIAATSCTRTASPPWLSVPLVTLLMQLCNETGGRDWRAGDIGAWHGPPGTGPLPRLAIWNLVSGAVVGTTAPLGPVPMCLTPTLLGELLLRGVASVEECAPAGGTGQVQLQEESNLLHKVCFTGDVALLEILVAAGADVLCLDAASNTPIHVAASRPHREEVAAKLLHTLLGRAGQSGATGAVNQKEQGLSKGPGLAEAEAAVAAEEARLMALTARNRAGMRPEDLASSRKVKKLLIQLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEU3</accession>
    <name>A0ABQ5SEU3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08241">
        <property type="entry name" value="QOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF4">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="19"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="341" mass="35664" checksum="9D832AD12E67E96C" modified="2025-10-08" version="1">MRAVICENLGDARTPLGEGVLKLSKVPEPELSPGTVRIQVASSSLNFPDALQIKGEYQDKLKLPFIPGSEVSGVVLEVANGVKNLQVGDKVCAVRQGGAFAEQVVAPAAAVWRVPDGVPLQDAAAIPIVYGTADLALRHRAQLKAGQTVLILGAAGGVGLAALQLSRLAGARVVAVAAGAAKADFLKAQGADVVLDSEVLAAEGTALHKALRAAVPTGVNAVFDPVGGAALFEALKSVAWGAHYLVVGFASGNIPKVPANLLLVKNTTMHGVFWGSYMSYQPRVLAESMQQVLAWFGEGRLRVEVYGRYPLEQVTEAFAAILDRRVRGKVLLTIGPSHSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF81</accession>
    <name>A0ABQ5SF81_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012435</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPO2/CSE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005043">
        <property type="entry name" value="XPO2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF8">
        <property type="entry name" value="EXPORTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03378">
        <property type="entry name" value="CAS_CSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="26"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="942" mass="103862" checksum="F1B8D57A0A23F6A0" modified="2025-10-08" version="1">MEVELQQLAAILQQTLSPDKDAIKAAEQQLKVAAQQPGYCLKVLKLTATPIDESIRQSAAVNFKNVVKYRWVPSEADLYGGAQPLPDAEKVQIKQLLVSITLSTPQRVSAQLSEALSIICAYDFPAKWPELLPELASKLATDDLSVVRGVIQIANNVFKRFRGQPSSNNALAQELDMCQQLFLRPLHETTVKLSGLFPSLSAAGVASVEPLKQLLGCIRLSYRIFYSLCSMGLSEATEAELGFWMDEMHKMLTYENPALGEQDAEKESVLDGVKTAVCQTVDLLLEIDEETFKPFLQRFAASVWQVLMKVSQKPGQDNLTMAAIKFLNTIAKGVFSNLFAADGALQQICESIIVPNLRVRPEDEEMFEMNPTEYIRRDAEGGDSDTRRRAAADLVRSLTERFPAEISRLFTGYITAALADYTASPTTNWRSKDCAIYMVTALSVKGRTAAHGATSITQLVNLLDFFSAQVLPELQNPKINENPLLKADAVKFVTTFRSLLPKDACLASVPCLVALLGSEYCVVHSYAAICLERLLSLKEPGQKTLRFSPQDLGNHLQPLLERLFAGFKLPDSSENEYLMKAIMRVIVFVGPAIAPVSQVCLQRLAAMLVEVCRNPRNPSFNHYLFESVAALIRHGTAANPVSITQYEQALFPAFELVLQQDVQEFHPYVFQVFSQLIELRPAPLPQIYLQIFPPLLSPMFWERSGNVPALVRLLQAYLTKAGDEVVAGGHLVAVLGVFQKLIASRAHDHEGFYIINAIVESLPLQAYVQYLPSIWTLMFQRLSSTKTPKFCRFFCVFLALFVCKYGAAATAEQLDKVQAGIIYMLLQQVWLPTVPSIEGTDEEKLVTVAGVKLITELPGFGATGAQAALWVAVRKGLVDKVSGGGAAGSTQQEDDDADFEEMQGYSAAYARLANASKVERPVLAEIADPKKYVQDALARVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFD3</accession>
    <name>A0ABQ5SFD3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="27525" checksum="6D54FEFB144531EA" modified="2025-10-08" version="1">MNNNKLDEPALLAGCKGVFSKSSYITIGNEGKPEEYGKKIQMRSAYGGKHLSTVPPKDGKTVDVYFEKKHNWISSGDPYLDRIRYKDIQTEKKRGFQTSDFSKRDEFTNTIRTEQWREQLKAEGQHAKKALDIFAEATGITSVPTSIRRAAEEETYAYDQVYEKEDPTFDGASKTHRDTKNRTMLSRERVQGELMTTTGLAFQAPDDHHKPEHGRKPIVRETFYRKTNIFFPEGCAADPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGS7</accession>
    <name>A0ABQ5SGS7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RecQ-mediated genome instability protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.770">
        <property type="entry name" value="RecQ-mediated genome instability protein Rmi1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042470">
        <property type="entry name" value="RMI1_N_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013894">
        <property type="entry name" value="RMI1_OB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14790:SF15">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14790">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 1 RMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08585">
        <property type="entry name" value="RMI1_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01161">
        <property type="entry name" value="DUF1767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecQ mediated genome instability protein 1 OB-fold" evidence="4">
        <location>
            <begin position="94"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="205"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="353"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="501"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="590"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="736"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="782"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="912"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="527"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="608"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="748"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="785"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="813"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1170" mass="119543" checksum="DB66DF09693FA51B" modified="2025-10-08" version="1">MMNWEARLRQQYAIAIRPEWLEQVVAQLRGAHPDFSTWTEERMLDNIFTAFLFCDLNQAGTVSLPLNTKEMHKEVLMGKFVLQLDEVVNMAAAARERYSDQTGNRCLKFALTDGCQQVVGVEHTHLPAFTWDCPAGSKVLIHNVPIRRGLLLLAPDNTALLGGQVERLEAARQRAVAVWTKPVVGRPAGSAERDIFAEARQVAWDPGAPAGAPQAGPPGAPPHAGASGAGTQAAVGMVPPSLPHAIGAGGQPPGAPQQALISLTSSAQPPGGGNVGGGGGGPAPLGAQVVGPAGQYPGHLAQPPQPPQHVQHRQAQQQQAAVPSSIHQGYMPGGTAAAGGAMAAAVATEAGGASWQSQGNPQTAAHHFYQQQQAQLQAPIAPMTQRQPSGPPMVPAPTPWLGRAANTEFGAGNNNGSDGDQSRNRGIIPAAAAETTAPRRGLAAVEAAAWMNSGGRVAGGGTGQEPGRAAIPARREGLRDGDGGGGGGALSSEIVILLDSDSASGHCPPQPSMDQARRNGPSNPNPQSSLRQQQQQLHLQQQRQEYPELHAQQHQQQLQEQNLPRRRLTVSTMQAASLLGASVAPSAESMQLGAQISSPTGPLPPRPTAATRANTPGAATFPPTAAATTTTSGPAGTGIELRKAALPLARSSPTLSVSSLGSVGRSGLGHVASATATAAASPTRSTAALGFLNNRFDALASGYAALGAAGGGGARAAARAEAGLAMEVEMVDLADLDDDDDYGDGKGNEGGSGALGGNGAAHTGDACLPQAVAREPWCAAEGSWGSHSLPGSTGRPQGKRPRLSSAQRNVDGGKAGSMGADGGASGGVQATAHAAADGAGGRSECAALRSAAGTAAAELVPETQMDYDCAHFGRALVEPVAEVEEWVAPGVDAVVPTATAAVGAHRTFPAGGAAAGPNHLHQNLSPRGGGDCPEDSMAVKLLDSDMTGDLRGSPSVPPRPMAAVAAEAAGDAQQESWVKAAGAGSNHAGAVWLCQVADMLRPEPDCPDVPVTFPMTLDVYGVVYELDGDRAVIEDGTDMMEAQLTDGALAPLLVGTQLPPGFAPGTTSLGLEDIWAMVDSLDPAVQACGQGLADRIMAFFRDFSGLIRLEFRGPPPVQPLIIQVTSDGRGSGADACGWLEGDDSCELYRTYLRTAGALPVTDMDRKDAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGY6</accession>
    <name>A0ABQ5SGY6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF289">
        <property type="entry name" value="GLYCINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="389" mass="41893" checksum="2D44EEC0892F6A12" modified="2025-10-08" version="1">MTIQVVIIGAGIVGAAVSHQVARIGAKVRVLDVVGQPGQGAATRSTWAWLNANKKHPKHYRDLNLAGLQRWKAEWPALVHSCGSLLFDDDDEASLSDPVYPGQHVGPEQLRQLEPFVSEEMGDRGAHLFPQEAWVDPQVVCREMLSKAKANGADVLLGPTHQVMGLLTRTEEHVARVTGVVTADGTTHPADVVVVAAGAETMPLAALAGVQVPLLHKPAVVAITSPLTGADGLPRQLTRHLISTAKVFVWQRPDGSLMLGDTRAHEDGSTAWGESLLQRAAELVPELVVAGARVERVEVAYRPWPKDGHPIIGPSSTCRGLYIATMHSGMTLAPEVARLVAAELRHWDVAAGVARGSEAELLEAQAVLRPYRLDRDFEEGARRAAYAWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH93</accession>
    <name>A0ABQ5SH93_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013827</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
            <location evidence="1 8">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the dynein light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019763">
        <property type="entry name" value="Dynein_light_1/2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF35">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01239">
        <property type="entry name" value="DYNEIN_LIGHT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="122" mass="14039" checksum="D63DF84F06AC3305" modified="2025-10-08" version="1">MAQQGVDEPQEPTGYQLQKQHYYKIITSYMPESFEQDAVQIALIAVDKYRQLKDIAFYIKHEYDKKYPGSGKATEGVYHCIVGKSFASAVSHETRQFIHMKVDTYHVILWKSKDTPFTAGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHE5</accession>
    <name>A0ABQ5SHE5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_013228</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF376">
        <property type="entry name" value="EXOSTOSIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046063479" description="EGF-like domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5 6">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI68737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="611" mass="68501" checksum="350BDC4BFD342AA1" modified="2025-10-08" version="1">MSWVSSILLAGLVLAVASPEIRGKGYYSSRLNWRQRGRPLRRRLAEEAAVAAPPFPIAINTSCLPGCNIKGVCNEELARCHCPRHFMGPSCSDVAPNIAEICANYGFTLSQCRKASPCFNSCNQRGRCVAGICHCQPGYWGMDCSLSWGPDGKVQLLEGKYVPRKDGLKIYVYELPPNLTSWFNIRRLDRPLHLLFWQRLMSAGLRTADGNEADYYFIPVNTRTELAPGMVEWTISYIQTNYPWWSKDLGHRHLIIHTGDMGLHDLPSEMRVRLNSSFSNITWMTHWGIHRYHPVAKWYPAHRPGKDVVIPVMVTTQGLHLSPMNPRVAAIAARRKRHTQRTGTFFFAGRICGDRKPPEEVTGACSPTRPDYSGGVRQLVYLHHRNTTGFKVVPGTKTYLQDISTHKFCLAPLGGGHGKRQILVSFMGCVPVLIGDGVLQPFEPEIDWSKFSVSVPEKDIPDLPRILNNVSSETLEDMQKRLKCAAQHMFYSSSLGAILGEDGRYDAFETIIEILRVRKEHPDVPPENYVDVDERFRKFIDCDLASSSAGGPDPNVLCNQGPDRQYKGMPTCGECQRSVADQRTGGTFFSWAGGLVCCSELDITKCPRVWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI30</accession>
    <name>A0ABQ5SI30_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013907</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045591707" description="Sulfotransferase" evidence="2">
        <location>
            <begin position="27"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="562" mass="62532" checksum="C1C5DFC8017DC90E" modified="2025-10-08" version="1">MSSSVACARRVTNVLVFLCCGFSAWASQQLSISEIFAHSRLLANFSNNVDNNLVQGLTEILRTPSNTRLRVVHELQHGRMAHTFLDIMKTYATITADNMPSWLQHLANFLDRYSRGVCPVHHGDMVLMVKHTASSGLPMLRRAIFAKLATAWLTTLGKRIPDIALEKLGEVILKLYRIHKDSVIQKGVMEYFHISKAGGTSWNSAAKLNGCVRARSAGSHVKGFGDECRWMDQNVYRNLTGRRILWGRWGRVSRATRQHGCRARYDYVASKGFSYISNEYTIQGGHDSILDAHICPQFVNVVTLRDPIKRMESNIRFIMMHIKVAHRRHGKEDRFNSTFCNASAALWEALGPPITDNYIVRSFLGVEGFHTPLGHISAEHVAAARDQLLQFDLVLDLDAGPEANDRFAQQGLGWSAALSAAHALDSSILSIKMQLNYSICHPEQTGLETLFRRQGPDQQLYRFGRIINQLDELWLDLSRALGEKPEQQQCGMLFRGSNNSALASAIARMTGRLSSAGKGGMIRPEGGQKQQGIPDGALDSMDADSEVEAEAEADMDGADLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIH5</accession>
    <name>A0ABQ5SIH5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013921</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="122" mass="13158" checksum="2378E229AC53BEF2" modified="2025-10-08" version="1">MAHVTSANLVYVTAKADWEACLATAKAENKVTIVDFFAQWCGPCKVIATVYESLAREHPNIYFLKVDVDSPEMTSITEACGITAMPTFQVFVNGVKVDDLVGASQDKLKALVAKYANAAPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIJ7</accession>
    <name>A0ABQ5SIJ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">VaNZ11_014494</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acetyltransferase enzyme. Acetylates histones, giving a specific tag for transcriptional activation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15614">
        <property type="entry name" value="PHD_HAC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1020.10">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031162">
        <property type="entry name" value="CBP_P300_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035898">
        <property type="entry name" value="TAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000197">
        <property type="entry name" value="Znf_TAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808">
        <property type="entry name" value="CBP/P300-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808:SF1">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08214">
        <property type="entry name" value="HAT_KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02135">
        <property type="entry name" value="zf-TAZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00551">
        <property type="entry name" value="ZnF_TAZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57933">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51727">
        <property type="entry name" value="CBP_P300_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50134">
        <property type="entry name" value="ZF_TAZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TAZ-type" evidence="17">
        <location>
            <begin position="531"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="CBP/p300-type HAT" evidence="19">
        <location>
            <begin position="984"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="18">
        <location>
            <begin position="1463"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="domain" description="TAZ-type" evidence="17">
        <location>
            <begin position="1524"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="687"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="756"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1246"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="763"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="779"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="GLI69795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1640" mass="179008" checksum="5848354400857F1F" modified="2025-10-08" version="1">MSSLAVSSASAAPARLPWQSDADTADRQMMTEQIFQIFQTKRPSTQQYNNKLPEFVKRLEEGLYRSANSREEYTDIKTLEQRLHDVAKRFVRPNQAAQPGAGVLQTGPMPQTGLQQPAAPPGQMSGMINMGMTGMPPQFQLPTTQPIQTPGFMSTSGGLAGGMGLPDPLRGQMSQVGGIVPSQAMPQLDPNSQYPQLLGANNAAFNPSTAMPQGVMSNGAPVLLRSQRDWNGNSAQQLLGGQSGQQTINKLTINGLLPGQLATGPGMIPQQQAMSNAGFVPNNGPSGMIPMGNGLVPSGGMVPNGGLVPSGGMVPNGGMVPSGGMVPNGGMVPSGGMVPNSGMVPNAAMVPNGGMVPNGVMLPNAGMVPNGGMVPGAGMVPNGGMVPNGGMVPSGGMVPSGGMVPGGGIVPSGGMVPSGGMVPNGGMVPMNTGMPGMSTAGSPLLNNMANFSGPQSVPNNGLGAIGPNLQGMVPVGGATNGAATLSGMIPVSSASTGFSTASTGVGTLGQGPSPMLNSTGMLGGMPDGMNPKVQEQYVQKQQRWLLFLRHCAKCRAPGEECQLKTQCKFGKQLWAHILSCQNAACDYPRCTSSKELLKHHQKCQASQCPICAPVKDYVKKTRQATQQQQAQQQAVQQQQAAQQQQVAQQQQAAQQQQAAQQQQAAQQQQAAQQQQQQQVQQQQQQQQQQQQGAGGPFISGQPSMIPNGLISQQQQQQQVVRLNSNGLGGQKRPHEMMNVGLGPGSLEGVSRMVPSIPTGMGLGDPLLQPLQQPQSQGPGQPPMMPQQPQQPAPKRPKTEDVLRQNTGTSLLETFDAKQIRVHVDLIRAAAVTQKAQQQQQPTNPEDACKVCLLTKLSFEPPVIYCSSCGLKIKRGQIFYSTPPEHGNDLKGYYCHQCFTDQKGERIMVEGVPIKKSDLVKRKNDEEIEEGWVQCDHCEGWVHQICGMFNKGRNNNDVHYLCPECLAYGYERGHRQRTEIRPQAMLEAKDLPSSKLSDWITDRLNRELERERIRRADQQGKAPHEVPPPEPLTVRMINSVKKKCEVKTKFQETFAPDGYASEFEYRQKVLLLFQSLDGVDVCLFCMYVQEYGKDCPAPNTNIVYLSYLDSVKYFRPEIPCALGPNVSLRTFVYHQLLIGYTEYTRNMGFEQMYIWACPPMQGDDYILYCHPSKQKTPRSDRLRMWYIDMLKLAREEGIVVHLSTLWDTYFEGGRDHRMEKCSATYIPYMDGDYWPGEAENQLATIADQQRGGKGPKKGATSSTGAVVPSRKAGSKGKRYGGGPATTDEQLMSRLGEILGGNMREDFIVVHMQQPCTFCRTHIRGPSAVYRYRVPPGATPPKAAPERKFEGIKLEGGGPSVPSGTVSSLTICEACFRDEENRVLTGQQLRLPAGVQPHDLLAEKLEEMHLWDRDPDGDMESEFFETRQTFLSLCQGNHYQFDTLRRAKHSSMMVLYHLHNPHSPAFASSCNVCQAEIEPGKGFRCTVCSDFDMCASCKFNVNHLHPVVPHKRALDETRQRLTEAERRERNEQLQKTLALLVHACSCHNTACGSNSCRKVKQLFQHAVQCTAKVTGGCQLCKKMWCLLNLHAKSCTRADCPVPRCKELKELRRRQTNRQEEQRRAAYAAMLRQQMATQQPRPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIQ8</accession>
    <name>A0ABQ5SIQ8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="173" mass="18016" checksum="1EB693DC1DD49B05" modified="2025-10-08" version="1">MTTPPIDPKDENANTQRALLVGLPLMIGWFALNVPSGLSLYYLSNTVLSAAQQIYLKKLGGATVSINELGPVTKPGSGRRSGTPAMHIKLWVPSTVMTTAEAAKARTEAEVGAASQEAADATAYEGSAPSFDAAGASPVTVAGLVDLSIVNRRCKRRRLSSLVQDGSNGFLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIU6</accession>
    <name>A0ABQ5SIU6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014377</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IMPACT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23821">
        <property type="entry name" value="RWD_IMPACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020569">
        <property type="entry name" value="UPF0029_Impact_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF25">
        <property type="entry name" value="PROTEIN IMPACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00910">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RWD" evidence="8">
        <location>
            <begin position="15"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="123"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="308"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="339" mass="35994" checksum="E165849E831EE4E0" modified="2025-10-08" version="1">MADGTDAADAAARDEELCALEAIFGDSVSTSREDSFVEVCIPHPQGGIQEVVLRVSLPSDYPSSSCPVAALYGDHLSDDVLAWAVKQLATLFTPGDVVLYNWVEWLREQEIFWAVPSAPSQQVLHNGKQQQREHREQRGDEGAEEEGATHGSSRRPATIKEGIREAEAVELLRAMRVTSGEPYVEKKSTFQAHVAPVRSFEEVAAVMDALLSVSKIRDATHNIMAYRIRSEGAGGCGGSFLQDYDDDGENAAGGRLLHLLQAADVRNVVVVVSRWFGGILLGPSRFTAINNTARVLLEQMGLVSGGGKGGGVAGQGGKGSAGGKGQAPAATISGKRAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ29</accession>
    <name>A0ABQ5SJ29_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014311</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF12">
        <property type="entry name" value="AT HOOK TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1324"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1561"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1365"/>
            <end position="1378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1624" mass="171547" checksum="82961F441224A5B3" modified="2025-10-08" version="1">MLRRRPPPKETVVSDDQEPPQKQFSNGKKANHISLRVLASTLSAAVLAAVNPEPDVRSQAYSEYLTTHVHGLTIGWHVFRTVTEGAFLASYGLAFNTLVVQVFWSMSVGSVLMYFVRRSIWMNWRESICVWTNIIYGVITVLKIFHLLDRLPNENHYMFKHCFLTMADAAFSQTPVHKQYAVQFAEAVIRVIVWRRYNVLANTPTSGVIIISLAWQIWLVTFSSMLQARHVSTFRRLRCASGSSNPAPTSTPVTSRPASCEVEPVETCTPCASTSTGPTPPLPALAAETLPSSAPGLPNSLRPVRNRRSSIGASTTAAAGTAAVAGAGSGARASCMQSPTEPLRLSSTAVPSSQKRFMRALRGVARGPSSYVLLSHQRRQVGGTHIASAQFYIPAANPDMLSGTWRERLTLAIAEGAPGWQVSNMCVRKGSLIVHLDLVYAPRGREAADVMAASPAITGPAALPDGVANNRDVGFPGVSYSEQPFDTAGACNPKAVLDEYIDGMGAESLLQLLGLDPDLPAGPFQPISVQVNDKLWNLPIAPDTTAAPCTSATTTTISNSAIETIAFQVEPETVACNSSTAALEAAPGIKSDVAPQNPVIATSAGHEAWIRLVLLPLQTAMRPPAELRSSEYNCFLSERVAALHLSYAAFICLTAVALPVVREVSTVNPVTLFLFISTELLTLASHVALGRGPWRSMRCGLVYAGVAIRTLQMAACTSGFLPHVLRDSDCISGRWIQKVMLITTLQALCMQSPVRSFFLVRMPSVMLSIYVHVFVCQTGTALQNAAILHLGWEGLLFYISAIFQLRHLEAFHRRRNPSGCCQQNPVTAAAKCAAEDKQQAPLETPALRVARNSHEETAVSAAPASGKSTVVTPASSIQAGEDGKTVAAAATVAAFVPAAAPGDPCDVSIDTPTLVMAPRRAARLQLHFTCAPAIVEELQVVLRHINPVAMSSGVHSAHTVRDITRALVAPAGAAAAPLHVPAPFAAELTLVASPADMPGLVHVELWRGRRRVCSLPVLLVATPELPRQSTGGSAPRADMSADSAYAVHMGIPGAEGAVGRDNACFWLDDVRRYVGSLRDQGHIAAADQFVEELGTWLAQAAALERGLTGSQNVSTQRVANVLYFHGYGSEFMYVKPRRVSGCGGRGDEAAAAAAVPDPLQDTLRQLLLCQGSMLVAVAVEAGCIGLSEHLVGLLMGEIGATLRGLLETRTPVTQLPLLHAAVKSGDPQMVDLIAEWSTSCGLKDIWANEAAVLAVSLESRASSAVGDTASLGAALQFSTGTGGSGFAAARRPSRLLPHVAQDISRRTSSTFRVFEVCRSGGLADAPADAGAEPRCHSVAGDKGSAESTNPMVAMDNGGSSYTETADSHAGPDLDKEGNDVSDESPFKIINFRASNRPAVSSMVAVGSVPRGGSSLASSGEAVTGLSETVVSDGSRSNLGLGGGSSSGLVDGGVLVLTPLHIALALGDEGRLANHILRTYPEAYELWRTSQLDMEVESAVNVGGGSSVRLEVSTSAADSLALSPQPLMVTESIASSTVLPANLAAVATAYSSMPGLISLAKASSTRRERRDGGDGMDADGASADDAMDVGSMKSISLSEVAFYEGIRWVNEDAPARRPGFEVVRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ74</accession>
    <name>A0ABQ5SJ74_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="159" mass="18057" checksum="F858F23DA87E3F3E" modified="2025-10-08" version="1">MMVLATNAARIHQKEEIQAARRRQAKSPQPSKARNSSRNATNSSKAAAKKVTNDGDVRPSTKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRRKKVKSAWEDVRCTRARRQRGSSGSKKKQELFEEEVSKDERSEDEQDDATSAREDDDNDDSEIEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJB9</accession>
    <name>A0ABQ5SJB9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mRNA (guanine-N(7))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.56</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_014236</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:67008"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.1.1.56"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.56" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004971">
        <property type="entry name" value="mRNA_G-N7_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039753">
        <property type="entry name" value="RG7MT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189:SF2">
        <property type="entry name" value="MRNA CAP GUANINE-N7 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189">
        <property type="entry name" value="MRNA GUANINE-7- METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03291">
        <property type="entry name" value="mRNA_G-N7_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51562">
        <property type="entry name" value="RNA_CAP0_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MRNA cap 0 methyltransferase" evidence="9">
        <location>
            <begin position="39"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="366"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="373"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI69578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="456" mass="50253" checksum="14E5C8228BD67AA0" modified="2025-10-08" version="1">MAEGGGGGTSMWEQTRQHYNSHVRDNFSTREALQQRNEGPAKELKDFHNHVKRQLIMRFAYKQDRVLDLCCGRGGDLQKWRDAQIGYVKGLDISEREVEEAKRRFMELGPKRSRGVAVNMVCEFEAVDWLGQRLFEDEKAGPGSYGVVTCMFALHYFFVSEASLLFFLRNVSANLRHGGYFIATFTSGPRVLKLLEGRPEFRSPMLRLARRWKDPHRPPTFGAGYICDIADTVTASLEGTSEGSLEYLVDLSALLQAAANVGLLPVIDYMDPVLATCFRDEDIDAPFKHFKPFFLHSLQLDYVEGKLPPQPPSSLEKASSLFAACVFQKVDSPHDVSVPMAPECSFMQNKQMKGGHMAHGLPMGHTAPGGHGQPPPGIGVPPHPWPHRPYGDVPYGGGQHAYGGGGPQQMYSAGPPYGYGGTAHQHGGQPSFGGPGGSDRHKRETGGPPGRSRFNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJE1</accession>
    <name>A0ABQ5SJE1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_014876</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF299">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="11">
        <location>
            <begin position="378"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="10">
        <location>
            <begin position="520"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="782"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="954"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="786"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="796"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="806"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="901"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="922"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="957"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI70097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1552" mass="160747" checksum="099EF7C57DD94F3C" modified="2025-10-08" version="1">MIRQSWRPPGEGEVELADVQLSDRRAGDLSTHGGYEPIGWGTSSAGGTTGGAMSSSGMGPYDSMAGGGIHGDGYDVHHDAGAEKYGDISKTRLLGNAEFPEPFSKTGRGGPMERLSNCLAARWKIILLSWLAIAVVGIVIALVVSLTVSPKSSPSSAAPPFPPPLPPISSPLPFSPAPTSSLMVLPPSPPPLSSPPLPPLPPAYLFSLCNYNSSALPEDIRAVPLSYNLSLSLPDYEAFKVMAVSSTQQLLARSFNATVSLLLNVTGSSTACLVLNAAGLVIKRVDVVKLLSSGNGGTVAGNTSSSNNSSSGGGEGALGGKRLLLGLQPQQQDPLEPQCLCGCNSTSIPCSASTIKPAGSESIIVDLAGLVMATGDLWRISFDYTSSIRAVSDGIGLFVSDPWVADGPSTDVLLTTQFEDNYARYLMPCFDHPLYKANFTVGVELPANLTALSNGLQLVSTPTTPNRKLVKFETTPIMPVYSLAIAAGNLTSQESTRSPMRLRVWGPSSMNVSGRLQSALRIAEASYSFYSNYTGKPLPLNKLDLVAVPGKGYAMENWGLLMFDTERFTCPSGNPSAWDLFQAADVICHEMAHQWFGNWVTCKDWDNLALNEGVTSYAEYDCAAAVLDYILSFNGSTPTAPLPVYSPGLPLAEPLRRLVVPPLGMLPGVHEGVVGRARWADENPLVGVPLDKDANAVLVYSKAATILDAAAGLVGQDGLRSALQNLLTAYAYQNADVWGLAKLLGKQAALKWNGSELGDQDAFASGIMGWFTTPGIPLVTATDVTPAPPPRPPAVAPIQPSLSQSPPVPSPPLPPSPSPPPLPPAPPQTPPSPLPPSPSPPPLPPAPLPSPLPPSPSPPPSLLSPPQTPSLPFPPSPPSSVTPPSSSPPLPQPSNPQSGVPFPPPPSPPPTLSPSGELSPPLSAPLQPPASPVRNLAGGSRRQLLANNEPLQLAQALPPPLPSPSVSSPPPLTPLPLPTVPQTTTLQLSQQRLCGWGLLPPEVVCAAGGTYTAQASKLQCPGATAAGTDQANLTRWWLPVATYAAFSGGGGTAISNMLLLSNASGGLAQRNLTYGLPSNKWILRRPGFTGMYVSAYGAATPGTSGSSKAHLADLLSMIRSASLDATMSASDRLAAILDAQAVLQDVLTTAFSLPASANSSISSGPVVEDSMLLLPPGSVPGAAAMSNGARLPAVLVAIDAALKSPMAMTGAGLYTLVQPALMALEYLQSLLATAGTASTATATCNADLQSWVLARLGPVANAITNGTIQATSNKAGSSTDRFLLRLAQSNVVTEAALWGDPGARNFSCSLAASIAAVSPDWLPAALAVPLGAPGGCSLATTPGAPSPGDAFNASLAQVFSATDTEVRATHLFALSYAGGVEQRSRMLTVLQDGVSPPNGTVQLSKFMVQEILGRLVSSAQRYAFLKANDTMTSPQSSAPVNELLTAALSGPSVSNGVLTLISAIAGNRTSLSLSMLEQIVGRSLTTEDQMNTLGALLCSNTTASNSYIANVAPEDLSAARSRILGRAQSRLRWIAKFLQPVCAFLKAEALGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJF2</accession>
    <name>A0ABQ5SJF2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="120" mass="12357" checksum="68688F98239FF701" modified="2025-10-08" version="1">MAYTLHLSVPVSIQIKGPSRSPPSIADRITRPRQLIAHASDPGAGHGGPSGGPPSPVASGQPLTSTPQTTTMEQKRPEQQPGALTSAGSDVPQRDIFIPAMVAVALVGYALVAVLAFLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJK6</accession>
    <name>A0ABQ5SJK6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="531" mass="59604" checksum="9C1706DC08BE95E8" modified="2025-10-08" version="1">METHSPVVCKLLVCCPNEEKRRIDAEINLSVVDFEQLLTDAAGAKSISEVSVQVLDSEDEDGKLVAQQVAPVQLLSRCTQWLKKEISAPFKGKAWNQAQKKLTIHVSLKQPSVQLVSPEVSRISIRKDHDRGEPLKRSRSYEQAAINKAVRDNVPYNLDSSLKNPRVLIAKDIQREPHLQPVWDKCFAAGFAQVADLQPRVTVDCFKKKWQKYATDYNAQRARYEKVKATHKEELACKPEDLEKKPWKKLWNKESRAAWRSFKYAQPDNPDAGPSERRIAMEDLNVTDRQQELINALLQRKQIESSKTSFTVPATPVPVGAGGTAQHTPGLGALELPKGTTKSGRKDEPPGTQDGRTSQRQKQPANGEDALMMVLAANAARIHQKEKEIKAARRRQAKSSEPSKARNSSRNATNSSKAAAKKVTNNDDVRPSTKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRRKKVKSASEDVRCTRARRQRGSSGSKKKQELLQEEVSKDEKSEDEQDDATSAREDDDNEDSEIEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKN7</accession>
    <name>A0ABQ5SKN7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014806</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000091">
        <property type="protein sequence ID" value="GLI70181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="18393" checksum="720F2B9DCD2D017D" modified="2025-10-08" version="1" fragment="single">MSEQLAIYRRCRRQYPGTAACVLLPAFCRRKAEASLRGMRHVHRFEPGTVLFNGVDREGTLAPWPCLSYSVDVWYEAPDGGAVGYGPALSALTLSAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVAEVNALGLSPESSDCPQVTLADGGKHPIVGRITLSLSLGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMZ0</accession>
    <name>A0ABQ5SMZ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongation factor Tu, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_015807</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04097">
        <property type="entry name" value="mtEFG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04091">
        <property type="entry name" value="mtEFG1_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047872">
        <property type="entry name" value="EFG_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009381">
        <property type="entry name" value="PRK12740.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636:SF2">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="81"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="770" mass="83901" checksum="35796659B581669B" modified="2025-10-08" version="1" fragment="single">LRSHAPCRCVLGLMSRSVARVLTAALGKSLHPLLNTSNPHGSLAHLKITGFGPHGQLDGWLARCLSTALSPVTEQEQQALSLIRNIGISAHIDSGKTTLTERILYYTGRIREIHEVRGKDGVGAKMDSMDLEREKGITIQSAATYCAWKDKQINIIDTPGHVDFTIEVERSLRVLDGAILVLCSVGGVQSQSITVDRQMKRYNVPRLVFVNKLDRAGANPWRCIDMARDKLKLNVAAVQIPIGLEDQHSGVVDVVARKAYYFEGPKGDQVVEGPVPERLVEEMEERRLELLEKVSEVDDPLAEKFLAEEAVGAEELRAAIRRATLALRFQPVFMGSAFKNKGVQPLLDGVLDYLPSPTEVVNEALDLTAEEERLTLPCAPSGPFVGLAFKLEEGKYGQLTYVRIYSGTLRKGDSVVNMSTNKKVRVPRLVRMHSNEMEDISHAAAGDIVAVFGMDCSSGDTLTDGVKLAMTSIRVPDPVMSLALTPTSAEQFPTFTRALQRFQKEDPTFRVATNNSTGEIIASGMGELHLEVYIERIRREYKVTCEVGKPKVNYREALTGRAEFNYLHKRQSGGAGQFGKVVGWIEPLPEDSPVTFVFENKLVGTAVPPEFHSAIEKGFVEAANSGSLIGAPVYGVRVVLTDGASHAVDSSETAFRIAAVQAFRQAYANASPIILEPVMKVEVTVPSEYQGATMGDINRRKGLILDSGAVGDDTVVTAHVPLNNMFGYSTALRSATQGKGEFSMEYSHHAAVPREAQAALVGEIKRAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNJ0</accession>
    <name>A0ABQ5SNJ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate epimerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016347</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058741">
        <property type="entry name" value="MurL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058740">
        <property type="entry name" value="MurL_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26298">
        <property type="entry name" value="MurL_epimerase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26299">
        <property type="entry name" value="MurL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MurL N-terminal" evidence="3">
        <location>
            <begin position="308"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="MurL C-terminal" evidence="2">
        <location>
            <begin position="402"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="MurL C-terminal" evidence="2">
        <location>
            <begin position="471"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="626" mass="69107" checksum="BD4BCB2F0CAAE1B8" modified="2025-10-08" version="1">MSSLTREELAVRCGSTSFDNMSMDFKNKQVIFTHSFRDQQGTVLEEFRTEVHFSVLQPRPLTQPEERALLAFGLCHSTFVFLASAKRSQLLQVRAGYFSPDELAFFRPALAGALAEYFYLADLDLADFRLECCCDPPLPALLSLPSNPVAAAEEPPPSEPEDAAPAATTATADTDSMSPTATPSSPRHNAATEAKATEATLPTLPTSPTSPRHPPRVLVPFGGGKDSTLLMELLGRMGAQEVGWVYFGEWVGSWEVYSKFTQLAAVSPAPTVFLAEQDTPCFGRLKELNLLPDLPFQDYCASVSFYTFAAAFVALQQDFDYICVGNEYSANAINTTHPKYNFPVNHQYEKSFAAECAFHTYLRNYVHPRLYYFSGLQHMTELEIAKRFAAICAAKYLDLIISCNWAMDNDWCGGCEKCAFVFLLFAAFLPTQEIMSKVFGVFPSKASDSVNGGGDDTRSPIVPGSRESLRGVYDMYDSERHVPVFESLMGLAGHKPFECVGTPEECLAALYLAYERLSSGSDASCSEDSVTDVDAGAGADRYNGAASEPKLAETDDSSVAKEQQQEQQKAGKRLPLLLRKHEDLITSQGKEEWEKIYAEIRLAQETGRPSVPTLYPDWFKLDMGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQB1</accession>
    <name>A0ABQ5SQB1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Inward rectifier potassium channel C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_016442</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF102">
        <property type="entry name" value="INWARDLY RECTIFYING POTASSIUM CHANNEL 1, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 11" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <keyword evidence="5 11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="14">
        <location>
            <begin position="57"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="15">
        <location>
            <begin position="176"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="372"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="518"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="396"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="518"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="554"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI71301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="620" mass="66962" checksum="844DE82861BB1F7A" modified="2025-10-08" version="1">MPGSPGDRFVRMRSDGEGLLSGAARRLSRLKIQFKAPSLLDRSAPYFSGVKRFGISKFQHYWQDPFHTLLNLPWGRFILIFFATYMIEFLVFALIFWVQSDRCIEGKNGKFSHALWMSSRTASTLGFNAIQPNPECTFTNLMVMCQVIASSLVNFIMLGLVFARFSAPFKRATTIRFSSVMVCYQQPATGYWCLSLRVANIRKHQLLKPALRMVLTAVDSITPSNYLFEHLPIDGHGTQETNLELGFPANVTHVITPSSPLYNLSVLEMDTRMMEVLVFVDGIDAMTSKNMSARHAYGTNEVHMNEVFQPLHLEMRGGSLGLDFSGFDLTMLASAELVNEYNSDPGLAERPLPEVQSHMWHLRHMTFKRLTERDHGHPSGSGPGARNSALGASGRGATSPTRGSSAYRPTSRVDGAVMSTAGGGGMGGGLSNPFLAAAGGGGGVVAASAETGTAGGAGASSWMGFGVPLQPSGVTVFGSAAAVAPALPPGAQQVPLPLSTFINSGQSGLESFADMMPMQQQQQHQNYQQHQQQPMYRGSTVSPRPQQLPDFSGAAQPQASQQQQQQQQQQQVVSRASWSLGQVLPQHHNLNSGTGSGGLEMLPTSLPAVEPRANGVGGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQD5</accession>
    <name>A0ABQ5SQD5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fanconi-associated nuclease</fullName>
            <ecNumber evidence="8">3.1.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_016863</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Nuclease required for the repair of DNA interstrand cross-links (ICL). Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.</text>
            <dbReference type="EC" id="3.1.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the FAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.1" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22326">
        <property type="entry name" value="FAN1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033315">
        <property type="entry name" value="Fan1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049132">
        <property type="entry name" value="FAN1-like_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049126">
        <property type="entry name" value="FAN1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049125">
        <property type="entry name" value="FAN1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014883">
        <property type="entry name" value="VRR_NUC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15749">
        <property type="entry name" value="FANCONI-ASSOCIATED NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15749:SF4">
        <property type="entry name" value="FANCONI-ASSOCIATED NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21315">
        <property type="entry name" value="FAN1_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21170">
        <property type="entry name" value="FAN1_TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08774">
        <property type="entry name" value="VRR_NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00990">
        <property type="entry name" value="VRR_NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VRR-NUC" evidence="10">
        <location>
            <begin position="1180"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="153"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="668"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1295"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1385"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="153"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="688"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="698"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="711"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1393"/>
            <end position="1406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI71599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1418" mass="147477" checksum="08E7A9DC8E564F47" modified="2025-10-08" version="1">MALRGQGSLQLHLGKSVFGAKKRKAGKKELDAVTKRQRTSAGANGPATDDASPPAGARPPLTPRLPRQQQPAGLLSNQGLLKKYQSRELQHSQRHPHQLHSPGLPTPPDGTGWIRIETNVVGLRFRRISSDAAATAAAAAAATATQLGGVDCLTSSDPSKPSLTSESPWTGGPRYRLAPEPTNPQDANAVQVLQCASLAPAADDSSRGNYNNKSRTVTTTSAAVAAAAVPRDRDLMVGYLPAVVAAHIAPWLRAGLLETEVHVCWHAREGAGSVPLVMYVRAVATGSDDRPGTSTTTSHAVVAATAPTSTVVATTAAATVTNRVSEARSTRGAVCGGIDASAADDASVDGCCDDDDDGDGELGAAHGRIEECGGVAGSGTSPVIGSEAGGTVGGAQASIQAALEAAAAAAVDWMLHRSSSSNAAGEVLRSNFMVMTEDVRRHDGHLLSPDEHLLLGRLLELPAPAQCLFLRLLLRKGPWFALTGIAYPECGDVAEAAEALVAAGLAARPQPDDWPQIAELLRVAEVRALAGPGSKPGKYRQTDRLIARRDFPPQFRSQQAQTQTQVPRKSSILAYTVSKDGAAAAAVRAGSCSTVAVPAAASVESGSIGGGIIGAPLSPSPAPPFAPVSAPIVDAADGAALLACGSVGGSSGNGVIVDFCQVLSGRADSSGGGGGGGGRGRRGTRSVGSGGGGGSGGRRASNGGSVNAGTVAGGGASSGGGGKTAAIEALRRRSKDCTAEISKWVEGRVGPCVRLSPAAVELVVRVQRLYFLAEGPAADLGRFLATERGVIRYPTYNLRRSSAAFSCRQQLLDYEQALSHAAQLERFLEAGDDDGATAALAPALAAVRAGSHRLVRWCGDATIPVRAVPGPWRWEGVADINLPAATHAGPAAAKVAAGGANVKAESDFPIGALPGGMRRSDGDDECLKEDCVQGSSELAARQQAGNKCTGLRSLESLAIGVSLLERRRLYGEAVGLLRQLLQGRCCPGRRGEWWMRMSVDLQHLGREEEALQVAEAALTDPWIRHGDRLALQRRVLRLGKPPRRWRRPPWAATALAEPREVVVSAIMMKGAAGVKSCFAVPSARGNGDSGGSSADGSSRMVSVEELALLHYGSAAGGGWRGVHSEGGVWTTLWALLFWEVLFMDVPGVFRSPFQTAPLDLDTDAFLPARREAVDARLQLIASGAALDLIRSGWSSRVGTRCRGINWERWTLEDLLQIAGCVGGLGLSVVCRLLCEDAGGWRGGMPDLLLWRPERGDAMVSEVKGPRDRLSDQQRAWLAALSAAGLRAEVLRIMEAPPPPHPGPLGQCRPEEDGSRQQAGAAPIHSYSGGGNVRGRQGARMRCRRAMAVPIVCENTTGETDGRTEVVGEGVVLAAAAVAIEAHSGMKDGTVAISDEEEDEEDDDGNDDGNWSADDSLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQG0</accession>
    <name>A0ABQ5SQG0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="124" mass="13112" checksum="61ECE863D5D9A234" modified="2025-10-08" version="1">MDTRPTSARASEDGFGSARMERRPRTHAVASHTRTLAQQRPSRRGPSGSRPLHSPRDGIDRSPNSLGGIHLFWRPGSDTEASPSPRLDGPPARLFLAGLRGAGIPGSRAPSSVRSVSPVLSEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQQ0</accession>
    <name>A0ABQ5SQQ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_017018</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048682">
        <property type="entry name" value="COG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048684">
        <property type="entry name" value="COG4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013167">
        <property type="entry name" value="COG4_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048680">
        <property type="entry name" value="COG4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20662">
        <property type="entry name" value="COG4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08318">
        <property type="entry name" value="COG4_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20663">
        <property type="entry name" value="COG4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00762">
        <property type="entry name" value="Cog4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="COG4 transport protein middle alpha-helical bundle" evidence="10">
        <location>
            <begin position="192"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="367"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI71719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="781" mass="82980" checksum="3FD70845CE8DB8E4" modified="2025-10-08" version="1" fragment="single">MATSAIDLNLIYKLTNVSDATKLLHETVARERAIDQDLDRQLSKRSDLERNFLLLNTPTAETLELVRADCEQLLQSVQGTAQLADNISSKVRQLDLVQGRVQGVLNKINLILDRTNCINGVQSAMETEDYEAAAHYISSFMELENRMEAALAAAGPGPVSAAGGSPASTAGVAPLSQGVVDASQAEDQRRVLMDVRARLEEITDRRFEDAVARRDHAAAVRFARLYKPLGKQAEGLQRFADYLKVVVGAQARNTFNALVDQLEGPQAAGRGKADFAAALTALFKDVAVCLEEHEAVIKETFGEAALLDVMAGVQAECDNTGSRILQRYSDSRRLERVVREVTGRRRGGGASGGAAAGGGGGGVTLSFGVSGQPAQQPGTAAGDQGTDHRAVEALIEEALRICGLCEEYNQFILGKMRVAAAAAGGAGHGGHESTGGAQAAAGHLPAAREAAFRSGAFNVSLRELLSRYVALEEYYLDETASMAIRIDEVLLGALTSSMVDDVFFILRKVGLRALAAGQFQATAALLAELNNVLANQFRNALQAKISVGPAKALAALPAQLTGAGVDVQGSPSLNDMMVAINNTDVAADYANRLRSELETHINGLLASASDREKARSVLSDLAKTGKDFRSMAARGLEALAEGLLPRMRTLLDEVAAMSYSLTEADYSALEAEGGWAGRLVLALSGLASLLRPLLTTSNWEVLFGSLLDKLSARLEGLLGRKSFNQLGGLQLDREVRLLISGLGDLTGRTVRDRLARLSQMAVLLGLESVEELLDYWGGPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RL74</accession>
    <name>A0ABQ5RL74_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit F</fullName>
            <shortName evidence="4">eIF3f</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">eIF-3-epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000106</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit F family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08064">
        <property type="entry name" value="MPN_eIF3f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03005">
        <property type="entry name" value="eIF3f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027531">
        <property type="entry name" value="eIF3f"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF6">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="5">
        <location>
            <begin position="15"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI58404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="292" mass="31983" checksum="6699DF4EFA0BB3D9" modified="2025-10-08" version="1">MAATVLLPSSSNVSVKVHPVVLLSICDAYVRRKEHQDRVIGTLLGVVVDNVIEVKNCYVVPHNESSEQVMVDVIHHKTMFDLHQKVAPHESIVGWFATGSDLYNSDALIQDFYSKESTHPVHMVVDTTLKDNKFSVQAYTSRMLALGDKVLATEFVEIPCDTIFGDIERVGVDLMLTGLNDPSPESKKETANKSFSDEAETLAASMGRLADLVARASEYVDSVVSGRVSGEPAIGRYLADTLALVPHLARSDFERLFNESVQDAMMVTFLSDLLRAHVALAERLGTAALPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLW0</accession>
    <name>A0ABQ5RLW0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000194</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053209">
        <property type="entry name" value="Gramillin-biosynth_MTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47643">
        <property type="entry name" value="TPR DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G12710)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47643:SF2">
        <property type="entry name" value="TPR DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G12710)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="77"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="667"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="937" mass="98757" checksum="61C2951062ECE53D" modified="2025-10-08" version="1">MVCPACAPSSGLPQRRSLPAPGDRQKRVPSHRCRHGAVHQFAAGRHVIAVHWILSYRRLSLQQLSHHAPRSLPRYYGPIALKQIPGKGRGLVALGDLAPGDLVLVSLPAAVVFNDGDSGGDCGRPEGTEDDSEGDDGEPRAHSVEALAEAVQRGPLTPAQELLLRLSFDGSAASLEACPDLSDLFSTVHRLDMHQVLQERGGPQEPPPNPGWALRVVQLNCFSEPCMDPVMALSPLMSKAPSPAASPACADIGETDFGDHQAPASIPGTNGGRSHKGVQMLAACPALPFVRLAAGWSGSREDEDTVALGLGLGLDQLLTDSTNSAGGMARARQTESEFGGGGGDSGAGRWLQRPGHTGLWVEHAWMNHSCAPNVCNYVLGEAMVVRTSRPIRCGEEVCDSYLGSTLAAPVKVRRRALYKQYGFVCRCPRCCVEEELLGRPLAARLHVASRLVEMRVTPLAVRLMRRVLKTASAWRRGAAMGSVSGGVDQSGVASCREEVVVRGADGSEDGAEGRETGEEVEWDEEGEQEEFEEGEEADTLNARLWAEEILGGALCGATLEGASSSGTGRGVHLDAETGQRNGAPPGSPPACEGVLRDTQALGGAAGGLLSGITLINDLYLSGDGGGSGGGGPCGTLWRLALLTAALSELWAASEALVRAALLPQCRSERTDGNSDGPTPPRERVQRPGEGGDAGVAVAGSSTMGSIVQEEEELKECVQTGAGPAHLDLCVNWALFCLSGGLDLELACRRVILMVAVGAKADAMEAEAAEPAAMSKEEAVQVRNPNGNKSGSVEEGVLAALHSWCESQRREMAVRRSRLVELAAATVGGTAFHVDTTVEDWAVRRTNGSSLIDTGVTPELRYALTCRYGMLAHRETQGVAMPSCVSSSLSSPTHRIGPVAEFEDDKRGMSSRFNLEVEGQGGERDAVLLAELWLKLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNJ8</accession>
    <name>A0ABQ5RNJ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000850</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="3">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="4">
        <location>
            <begin position="297"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="646" mass="72298" checksum="D200975520785550" modified="2025-10-08" version="1">MSLQPNGFSSLAPEIQVLDGVVQPIWLYSCSNQKLIWANKAALRYFNKTLDGFTSYKFTRLSPEERSSFLVLNTLLRDEVENGGLDQVISGFGHSVLPGVIPHEELSNSEYLYKQFHLLRPLSSYARVTQIQLISGPNLSLPTVNATTVKTVRRERNRQSSADGRIVNGTKVTSTVSSLIDICDRILKGLEVDLNSVEAVHQAVLRGDIITQPTRIEEELVSKEELDQDVGLNLLQLLGGKKLGERFAPAPESYDGEVIAGSMPGGSRSGGPTVAASVMAAAKEEVDTTGDEEKDVVAASLMPAMEAVLATVDEWRFNAFRLAEVTQGHPLSALGFWLMKRYDLISVFQLDATKLARFLRKIEDGYPDNPYHNKTHAADVLQGMHCLLTRGGLHRRLGEDVAIFASYLAAITHDYEHKGLNNDFLVRVGDELAFTYNDISPMENHHIAAAFKLMRQKDYNFIKKIPREKWVRLRRLLIDMVLATDMKQHFNILSKFQSKLQVKLRSTNFAAPNLSHLESPSHDDPLPISDDDADKSLVLQVGLKCADVGHLAAPWEVHHRWVSGLEEEFFRQGDKEKSMHMMVSPLMDRCKDGITKSQVGFFDIVALPLFYSWASVFHEALPLVRAVEDNCNRWRSLEMGRNAGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNN5</accession>
    <name>A0ABQ5RNN5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="125" mass="13150" checksum="A41265BB7343A89C" modified="2025-10-08" version="1" fragment="single">MSGREDSSFLGTASQPDAGLAARTTFAEPYTVGTRVMQDPIVLQTPAHLIVPLSTAATTSEGAGVQRAPVTPVAQDPVGVPEVMKLSIPVPKLNILVDKKVKSTGCSVADKVCAYVRDVRKYLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNR8</accession>
    <name>A0ABQ5RNR8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903">
        <property type="entry name" value="GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903:SF1">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="649"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="912"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1105"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1352"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1749"/>
            <end position="1775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1909"/>
            <end position="1930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1944"/>
            <end position="1968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2061"/>
            <end position="2108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2157"/>
            <end position="2206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2221"/>
            <end position="2256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2299"/>
            <end position="2380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2412"/>
            <end position="2433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2481"/>
            <end position="2505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2533"/>
            <end position="2636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2852"/>
            <end position="2874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2890"/>
            <end position="2953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2976"/>
            <end position="3026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3120"/>
            <end position="3154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3184"/>
            <end position="3206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3230"/>
            <end position="3461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="660"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1123"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1352"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1758"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1909"/>
            <end position="1920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1957"/>
            <end position="1968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2093"/>
            <end position="2103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2227"/>
            <end position="2237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2238"/>
            <end position="2253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2308"/>
            <end position="2317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2335"/>
            <end position="2347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2355"/>
            <end position="2370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2481"/>
            <end position="2497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2533"/>
            <end position="2547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2550"/>
            <end position="2567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2603"/>
            <end position="2616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2896"/>
            <end position="2907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2988"/>
            <end position="3007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="3120"/>
            <end position="3132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3270"/>
            <end position="3305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="3317"/>
            <end position="3326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3331"/>
            <end position="3346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3357"/>
            <end position="3376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="3422"/>
            <end position="3438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="3442"/>
            <end position="3457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3722" mass="379957" checksum="D984DD23568E9477" modified="2025-10-08" version="1">MPAFNIDRELNIKADLEETGEPGIIIRFRLADTWQDIQEVLAKAHDLPTRRPLHPEAYEYHLEALLPPGLQLHPLPPSPPPQPQDDAELGSGTEARSVGSSSAWGPRLLQVADESAWRRLQQLLPGSRAELANWQLRASASEAKSAAYVRHLEGYTGMWEVAVATSGASPLTTLAASNATANGILGSTNNGKAGLSAPDGLGPAVDGGGAVGGVGPGGELDGCRAPLDGVWSEEEVALLARCLAEFMRKGHLERVGAVAAGCCSLATLWWLLKQPNARIRPAATNKGPRLSPSEPAAGEPGSKQQQLAIQSPRVGTGNGEGAGLDSGHDPGALPGGMLTIGGPTMAPPSQQQQQPAAAFRLQLRKKDRGARPLAALDGRSGGGGGINTVSGAGTNSTLMAVLLSPNLLSNGGAGAGGSGYVGGVSRAVSLMMRDILTRVATTGPVLLRQYKGSQVALAAVWGVGLLSEMFRNPLLRDELMVAAQLGASQALAAAAGISAGAGGPLVTGIIGSPQKRRVTLAGGSSSGGGALAGAEGLRMAPNAVPTTAGPSAGAGGSNLGIDLEEVHWLLLSFAAFPEWLEARSAPPWDLQPMGRAHAATVIQSSMRGLVSRRQTKRLQSQVSRVFSSFRASTASPTPTRAPSAAIAALQPRGQSSIKHQSSTAAPTPSAQQPPRPAVAAPVPRPRGLSLKWEEELATMSASAIDAIDRYFDLTPGSHPGVVAAEALLALLVSGGPLAREHFAATVHPGQLVRMLDTTTVPPRVVHLGVVLLALALRPGLGAEAFFRGAGAAAVAAAPGPPGSALGPCFAASSDRTMLAAVGSGGLGLVLELLRWLAPLTVLEAHERRLRYGASERRTYQALRSSTRPYAPPPLVDWRWAGSGSEVAVHAASAAWALSGQLAKLWAAEAAAKKPGAHRTQQQSQPAAGSGWGATGAAAAAGGGSYSAGFGTGGGGAGGAASGAGMFSSSMPMSSAAMSAAAASAAAAHAAALATARAAAGPQNAFEEAAFRMMAMTVTCLVSGRIGPGTHLLVCGLVQLVSEPAAVPYMVRAGLVDAGQRLPDPPLPPPGFGTGTGATAMGNSSTLATAAGVMGVKQSPSLRRMLSKSAGSMARSQHSDEGGAAEGFGGTNAHGGEEEGKDVEVQARAATSLPGFAGVAPHPRGVVGLLMDSDVVGFLDIALSIIGLVAQQGTPYDPIKGEAPTSGIGVTGAAAAASWGPARPPAASGTAAAGGGVYDLHGAPMSYDEAGSIRFPVPDLDHADIPARRLLMAGGWGAVCSCLASAGGLSQTLPDMAAAALMFLAKEVYLWAEVLTAQAIRRVAAEEAAKAAAEAAPRRRHYRVRIAGPAATIATTMPSTSSGTGHGYQSASDSSTPRDHTRGNRNNESSAGDDEDPLGLDPELPCPAEVVALLRLLLGDDFRTLTAALQALTQLLGRLHARPLQYACLTAYHLAGLPVLRNPLGKAGIVAGLVGVMRRWYDLPPSEQRAFRATGEWAMAALVRLTAEPGGERNRYRLASSHRDESPAAAAAAGQGVNAVKGFFSGFGYATSYVPGVAPVKRTGRTGDELVRGMKWGGGWGWGSGAGAPAGSGAGAAKAAAKFAVPDDDDELARELVGNGAAPRSASDPIGLLVQLTSLRSRSRSSYVHIKMPSLLLLRQFAGERQYRVRLVTEGAGTALAAVANDSAYDGLTRAAAAALWMEVSQTLEGRDPLWWSAMVEQQEDRSELLLDDEIGFREVVTRIRNGAPADAGKGGAASGSAAPGTSAPVTPVGGGEPARLRALSRMIGSMLSSMEAEQQLVGARAAAGLLCEGDSAKSIFLAAGGMGALLAVASRPLVQPGVLAAVCTALLNLSSYVPSQIDMCQRCARLLLHINAKYTGYHGYLIAQRGLAAAAAEAAKAGAAANAEDDAGAGAQGAPSSRRRRSSSGGSVLSYGFGHGYSHSGVDVPNSPRRGRASSTSGCGSGGVCSSVTASDEVTLPSSIKLESLGAVEEIIYSTSGALHNLSRHPGNRDAFYRLELLVRTRGAVAELAAETAARPPPISGMSRQQAAAALLSGELRADMPLPSPPSRQGGLSGSGTRGRDNGGRSDSSGDDGGSDNDDGSGGGGGVATAMWSISSASPFVPYHLRDEVVRYVSYTSYLQEVLSRPLHAPTWGGGGGGDDSRSQASARTGGGSSSLYSSIGPGGSRASSKGMGRSASGEDNDVDRFWAGSVAGSGIAGGGDDGLGDGGDDDDVCSQQQQQQGDALGSGDPAFPRLATLGRHLSRPLTTMWAPLLVDRHDGVAVPLFGLRDAVNGERLESGAGTPGSNTRTQPGPGSGIALPWQQHHHQTHHFYSSQQQQQQQQPGLGTGPAGSVSGGLAASAAGGRSLAGGASGGAAASVTSATTTITHLPAPPSATRWSPAVRRLRPVGESGADGAGGPPEGAATSEAEVVTSAVTMAEPPHASELVDGTVTLRVEFEACGPHTTVTVVRNFRSTTTPRGCRMSSNGDSAGGTSEKAPATTMTPKFAARSGTAAITASEAGATMGAVSTSGTAPLSDASSATGLRRPGANTRPATAPTTRGTMLAAAGVEGSASALPPRNGAAKPGRIRSQQTSQPQSLGPVSSGGASSVSLGGGTGGRREGDGNSGGGGGSNAGAGCGDGVCVPAPAPSMVQQVASLVGSMDTSKADRLAREISFSLMSMPSSALPARSRSRTAGFSSAGGNALRQSVILRNAERSKRDVGTGRSRSLLSSAPSFSAASVLSVATSAASASAAPSAASAHASSAFGMDGGFENEECGARKKGNGDPGRFGCKTPAGQQQLKDFQAPRVAIWPAVPGSKISDGLYPTYRLETGEPVHIYRRRKDRVDELRPAGLPQSSAPDATAGSYQREEAAAHDLLAKAGEVPLEPDLPDLPEPPAPPRPDSSTLRDPRDFTSVIARVTVRPRPGCNISESDFMEDKSRSPRARALSRSLTRFGPQSLVSLPALPPGVVFPGAGEDDSRAPSRSPSGRSSLPSHSQPQSPRDWELEVDEEGEGRESVDRPGMELDIFDLDWSRSIVRPRLKHLVTRNCPGGPAQVEELRAFMREQYWWLLLIFHYYAALYAPDAADPFVLQPAAWAVLTEQTGLTADSVLQRERADQGPDLRAAEGAEEGEGTVAGGGKEGPEVVPGGRALSAAELGRMFTAAAAIGGSTAAATMGAGNSGGGRSAAGSTHKEGTKSGNQAALNRLQWIELLLRAAAAKFLQTTEPPSPVGPRAADRGGGGRGPSPVMSRNSSISPPRRQLSRSGTGTVSRGRAGTRAGAAATAAAAGPAANTGANTVSGGGNVVKDTAPGGGGDGGVAAGPMSPAPSPSTPPQLPPPALKTSTAAAGVRSAGAKSPRAAAAVSVASGGPLPGGEGDTARGNRITSRQPSVRWKLPERALDEDEDKRRKGGKVADPLERPGREMSAGERGGDGGGAEDEVGPGNDGDGDNEGLAPDLVSAVRRLLYECFLPNVAPGALVEPHNFRAARLCQPDVLQWFQQQGPLLKALYVHYRDLGPTPGSATSRSGAAIVPGAGQGGTERRRLELSSWLELVGEGRLLSAHFTVREARLAFMASRARYAEELAAAKGDIVNVARSCGLSYAAFLEALGRSAELISPPPPRELAKLGFMGEAAAAAYYAAARRDPAIMLPPRHSAQLHTPRSRPLVEKLKQVMSILLHNLMDLMGCKTGEQLLMKLRQPRAQPRARAPRPNRAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP17</accession>
    <name>A0ABQ5RP17_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000736</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="962"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="domain" description="BZIP" evidence="4">
        <location>
            <begin position="58"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="443"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="554"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="893"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="258"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="403"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="567"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="987" mass="102692" checksum="2500071D631CE3C5" modified="2025-10-08" version="1">MSRGRRRDIAFVDSDYEVDMAAEGRRGGSQSPSPEAHARVAKKPRPGRTTSEGTSEEDERRRRRLLANRRSAQRSRVKRLEEVGVLENLVDNLMKEVDELRAELGTWTGRHQELLSEQSKLAERLKEEAGGTDIDILCIAHIENDDDWEPSGDNKQEQQQEPQGPSQQLPQSQSPAPPQQLPQPPHQQQQQQLQQSPPGVTTPQQTQQQPQPQPPQPQQQSPHLLEEQQQQEATSSSPPAPAPEPSPLPPSKQSSPLTNQHSENQSLREQQLPPPPQQQLPPHLSPLLPPPPQQQRRDGCRPRANTAVMQQHQQQPEGSEEVAMECISPGRLLDISTTPLHETDNHRNHHHGQDPVGPRLAGQRLLLGLPGAGRPVSVRDSRMDCRDSGTGDVENNCGRVSSGDGGGGGGSGGGSAGVAAAAATAATATMTVSLASRTICDPYSGNPKVEHLTDVEGGSGTSADSPMVRRPVAGEPSPAPTTSGPFGDSAGLLPRGNSLPYQADVPYGSFRQDHPSAPPLQQQQQQRVVPPRETPGQTISAPSLAVISSCMQPHRIDHPSYRSGEGNSSGGGSRSGGGACRTLPYDPNAAAAVTAASPARVGSSTAVGPPAAAAAVILGDEPPREAGVSTAMMPQPTCSGLNPPPQYRVGEGGCAASTVCRGWDTLDATSPLSYGSKEPPVPPAAAQPIGGAMSPVLKYGRTGAVKRRSSGSGGGGGGAAIPLPLHGPLGALLEPHIQLNHPHTAVLPSTAASRPRAVGLQPSNLALVSGVVATTGGGGRGGGLQQLPPTMPYSRLAPSVAAVPAAGAPASVLPHVPSGVSGHHHHMTYMNPVLDGAGMLAPTRRPGDALSAGGAAAGSLAAVPVPAPPVPVTLGHAVAVPGGVRAEGQALVPGVHPMGSADSTSSNSSHRRRSASSDSERSLALSQREASMRPLKQEIVGAGPLLHPHGALPPYSGGHSTIMFNSLLPFPCIVFALSFSYYFALRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ79</accession>
    <name>A0ABQ5RQ79_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="552"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="694"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="917"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="983"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="708"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1017"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1110" mass="114777" checksum="4DFBBA4288716A72" modified="2025-10-08" version="1">MSFVCSLIMLRRLCGNCGRLSTRVPDDCNEDTKFKRPNDHGNAPITCANEAMPSISSPSAAPLVDVDALLREAASVMRPQSDARGHGDLDLLALVPFRTLLSEIQLEERLGSGAGGTTWKCRWRTTIVAIKLMVSTSVDQLRASTREAVCSRIVSHPCVVQCYAINIARLTEQDMDLLATTGFPQIATHVEAGGTNNSQGPLLCLGTSPSVGLGAAAMQSHIQRLDQPQSQSHLGPGSVLAQKGDVPVGLRVQESTRIRSFKSAEAAGAVQTLQQSGQLEICSGTVTGATEPPGMEMVAPRTRIRQEGGVSGMQDATALTMASNASVPPPSPALGNPNEVGDMNDSVLLLLRAAVGAPLSDRLEHTLADSSRDFDTADAQGTRLELPRRQPQQQQLQLSPSLSFAPDHDHGTVSSAAATSDAVGGSVAVADTAGAATVATCGVPAQWPSRSESLLLDLDPGDQTAVFREELLRLGAAPGNFLTTIIMEWCDGGTLHDEFTCRWPQPGRLGLRRSGLASGHLLRGLGPTVGGGGSGSLGDLGLQNCSVASEQLDKLRRAGSGGAGRAVNSGSLLGPAYASESAAVISPDTSPEVLCGRGSCGYASGAVATAGTASPRSMYSTFRSSMPGLFPLSSSALYNTLGGVGATAFRSGQQPHLGHCHSGHCRPSGQLSSVPAISFARLMMLQTTQNQLLKHQPVGGPPSAVGSTGPVPDAAVDAATGGAGNGGANSSSASMWNAATSGRTAVVLRCALEVAQGMSYLHSLNLAHGDLTPKNVLLKSTATSRRGFTCKVSDFGLSNPTNTGDCRPADTSQWGTLVYMAPEVLTGRGTLKQADVYSFGAILWHMCTGRPPHQDLRPAQILVGLATGDLQLTWPDWADPGVARIGKACMDRDPSKRPQFEEIVRFLLGEIATRSRRRRAANRRVCSPSHGSGGGAAAGGLAAAPAMASSPAYSTPAAAAAVSMTDTGQPPFSQWQRSLHPSRIAGGGAATAPPPGDLHPSSSLPPPSPFLVPPQPPQHIHSGAQQLRTLGGCSKSQPRQCRQATHVAAHCCEWQPVVMPLPASGSISRERAAASLVTATAVAGGVATVGIIGGECSSGSNGTAVDTLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQK8</accession>
    <name>A0ABQ5RQK8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="759"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="840" mass="87538" checksum="FE9A67FB82B37C10" modified="2025-10-08" version="1" fragment="single">MPACGRLLDLPGPVPENIVSCLNPATRKVFRLTCKEARQLVDDTVQSAIVDVGNVRVLCGPWRPLHITFPQLRHLTLCLRSTGTITWDTPQSAVAATSVQVSGRNPDGPGGAAASFSLRLLRDSVPRLRHLQSLDLTQCHIDPTVWSALCDGVRGLPRGLALCLHPAAALLFSGRTWGERLSVMMSQLVAWNPDIRVRVMACAGSGGSGRGSGGAACWCDVDGEAGLRKLAELPYIYELPVCVWSRAPCQPDWLLPLSSLTRLRRLHISCCNAADPWDHAWLAGLSALVSLRELDLGRTDSPLTLADLAPLSACSALTALSVGSLTAAPSVAGPFQAGLPLIPLQPQPEGVGVEGDGAAAAAAAAAAGVSPLPQIRFLEIRCCLAVGSLPLGELVPGAVSISMCALTVGGSGNGNGINGHSVRVVSGHVFESLLSPAVTLGVAMALPPAAQLLMRFLSPPRLGSQFRSQLQQSRQHRPAAELLPTLLPPLRAAAQLHYGLRFLELVQQEAAVITFGRVLGGTGRSPLPDTAMLGCLTGLEHLQFSGWGKPGGQLPALLAAAQRLPRLRRLTFRDCDAQCFGWRASRMMAHLALGVGLGLRPLEALGALQLTSIEAVEVLGAPSLRLLEQLALACHTPQLRCLELHHVRHWDERAFQRILGTASRAAAAAAAAAVKASTAPRAAPTLIAAGGVVGVGGIGCDGGGGGGGGDGGGGSLPRLRRSFMARAAEAAAHAAAAAGAAGCRPCDGSGVILPREVRRLPAGWEPGTQARAPPRTSGSETWTCESADRRGAAVWRSVGAPEAPAGNAIAGGGVTRFSTRGCREVEGNCRWRRLSHIVVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSP3</accession>
    <name>A0ABQ5RSP3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002742</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF60">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="256"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1014"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1082"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1713"/>
            <end position="1745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1773"/>
            <end position="1811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1880"/>
            <end position="1922"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="514"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="605"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="799"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="914"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="444"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1016"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1037"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1054"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1099"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1169"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1195"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1208"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1222"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1252"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1724"/>
            <end position="1744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1779"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1884"/>
            <end position="1893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1912"/>
            <end position="1922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1922" mass="204224" checksum="E8826F72ACEAA89E" modified="2025-10-08" version="1">MPASNMPSTLPAIVTPRIGPGAQELNGSYPDERGFALSGLSAASAALRGALFEATGRNSNGTGNTTGPMAGRPLSATTIALMMSPGPVGPHSQPPPLLIPVAANGTSGASTRGSPGSILGFLSGSGPGAVPSGASGGGLAQEAVQSAAALRTAAEGLVEALEAAVRAREAQNRAMLTATKESVSGSGPGPGSSSSSGRPASARALDRALAQSTPARAPRQPSAGHSGLSAIAATPTSATEERLGWVRTLVRQTEGGQAYATAAEERERERERSTTGGDNAEPATPGHVRRGPLPALRPSRREDVVLLQAWLADMLQQVAAIGAVRQQQAAATAFTAAVAASSAAASGSTAAAINNPIAAAAAAASEPGLMAQHATELADASLYVVGVAMEELRRQVAAECRERGELLGQLVDQQAALVALRGTLATEGRVADVSAQWAALLAEREKEREKERERGKAAHGAADAAPDAAAATANSVAVASSLATQLVTAHHAAEAAERRFQLADAALAGEAGRRAAAEQQLEATRELLRRQEHEWLHHRTINQRLENEVQELKDKLEAATRNAAVAEATVEAAREQTRIGRDDVSQLEAALARMRQQYEGNEILVAAQRQQIRELEAALASLREQHREQAVALGKVGKVTEKYEDTLSDLRADLATMQEKAAELEADLTCRTARLSEVEERLATSDHDLSLVRAQLSATEAEAAKAASEAETVQRQMRHEITDAVAARRQEETRRVEVEKETTAMVEMVKMLGRTMQLQGMEGVPGITDRSWETTGPLGAAQRVLGLATQHVATLYGTREDLTRELRALQVKLAQEKEVTSKLQRDLYDARTTETRMLREKEVLEMKVANLEHDLRVSHSDATKSKAAAQDSEVKLKMQHETIVGLQAQAKELVPLKKRVEALANDLDCSRRIEEELRNSLDVTRRGLEQSEEQSRYYQNEVHKLQHEVGCLQDSIKALRVKEARLSEVEALNKDLDKQLMSLKHQLDLTAESHTDTIRQLELAKFFLQGLDGSNAPGSPTRSSTSANASPSRDVRAALSPSSHSRSPSRTLTMTYNASQSGRFGSPPGRLFRASAAALSSAGPSAQIPDGTTRPTPFNVSGTSSNGANGGNVNPSASGAATAATTTIDNPSPFADAAPYIGEDGRPISAVSSRPTARVPSPRGIPTGRSRNNQMGSIAVASSFTRPGSAITEPVPEDDEYEEVETDGPGGTSEGGGIVPTANDSTSAQPRQSRVSSAGATSSRREATGTVGAASSGGRSSRSPREMASRSPRSVSRANSMGLNLETRPMSGFGQHSVVDDDYDHSTEVSTVVMDIIESRGLTTRINRATHKWSSVKIGIMAWFSRRLRERLLAVSEQLAAKDLEIAQLHYSYVNEMKQREEFICTELLPTLMPAVESGLKDSYREVQQLRAEMPLARRDLDALAGSCAVLGFSLQAYRDRETHAVCVSCQTDSDCPDDWARFANHLPPLPPRLSLSLPALVDTIVRIYVRASGTVEEVPTWGAPRHDTIHDAIMAHYTSQRAPGTFQLELLKDPESPISRLLSSSISQARNTKVAGFLYFLNLSPEGVHWRSPTWHYFLHVLHCLRVLLSGTWRIVTKRWATPEGVQIPMPAVMDLVGNLFNVQSPGDLDGALSVRLAAIVTPGVAGPAVDLDALLLLLVREYNAGNCPRNALLFPRRLTDGQLFNIFNSLGHNTHVAALKSALAGQYIGPAAPSSPTTSIKRSTSPGRPTAPSSPSRSAATAMSLSPEASAAAFGAGLLVAQGDILHPYGDIGSDSARGSNAPGSPTLPEQQLFGNAPDMLSPLPAQPQLQHGGEVREEFDAAEAVGMTRQSVRWGPLMPMTGPAPIQPAGNMTVNFQISHDHHATAVVQQAPTLMQGQGQGQAAGSSTQAWGGGAAGYHIDSGNRLTALPPPPPRPRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTB9</accession>
    <name>A0ABQ5RTB9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027993">
        <property type="entry name" value="DUF4495"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14906">
        <property type="entry name" value="DUF4495"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1103"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1163"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1103"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1163"/>
            <end position="1185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1337" mass="142017" checksum="33E7496DA023F857" modified="2025-10-08" version="1">MWHSLASFADVELEVNAEAAKKSYEKLAIATWELQKHVKELLSEYPGTEAAPVTAEEPTNLEGAAIRHAAHLLTSLCSLPAQGDAISAPGAIYTPKGDARQRSLTDAGPSVFEGAGALRAFLSTDIPSMRWDAMAPSVKCMEKSSQRPSGTSPGRGAADPHRQVHNLLHVRPGQALKVDPHSRTGQDTDDLRAWVLGSLRGLHPDVSLAEVHEALLKGFVRYGALPPREDRVWFKVYGNNDIMARSRQLLSGLFHTSRTSLEAWASITQAFAHGLTQPDGEATRLMGTWSNREGNGSNAFDVTPNSYQKYLKDLQDKTSHLVEELLRDGLEEEGSPRAGLSLAEKDLAAYRLAVTPCTYLQVVGQKLLHHAAYQIPCMVELPERIPDWTIRSLYGGPFAEAVKLAVQHGVASINEAFGPRGEGPGERVRGNQLARSLLQSRADKSDPKRPEPRRQQQQQQLLQGADTAASHSSVPGGFGGAGGRQRGVVSGGHGGADGAKDGKGGACQRALNYARSAVELLAEVERDIQGFERGFIAILGGSATFPRRPGSVDKLLQQQRLVSPQCLTAYLSVVREDVARAFREMSSTLRVFLYGSVTSAVQVSLAVDDPYRSPCPVPLTSDTWPPQEDGYQPLNLSAGQIHCRGSTQARVEDAQSLIDPPCNSSSSSSLLLSSHVADDIRKNAVTRVGGDPCTVPGREAEGTAWTGDRSMPLLIDAISHRTLELVSWMTIHFEQLPEAIQGVLVGATLECLSLLMDRLSFVCTNLNDMPGSRPLQLVFLFHSAACCVRRRLEAMQPWRDAATAGRPCDGQDVELVRQHGEALVQADELITSIREHIVVMYMQVVYDVVLSSVDRTNWCAFRPDVMRGQGAGPAVRVWHSMTLRLLYAAALHASPSTAEWILGQVLLESVSCLVQRYLQLGPTVQMLREFVGDAVHLTASVFLFCQPIPVTALLPRAAGTCVESGAGWAPQPQDVQANGSRTEVFSLRVPFPMARALAESVRELLTRAALLYLPGSRFEEVVRKAGLLECADATVEDEPAPEKPGQLMRALASHRLSATSSVSLAPLPALRLSRVEAVQLGGSGAKPLLPGAPLAESQVTNVSDAAAATGSSSAQGSHNPSSSEDNALKAAVAAAAAAGSGCPSDGNTAPACSTVSLGSSDPACSPAPACSPAPGGSTDPGGSPSLAGSTDIGGSFRAGGIADAMVTDEQRLELWSSWLSVGVVRALAGPAEALELGSGRHATTQLLIPLGDRMTDTAPAGLGGGDGPTEAEGPIWRSCLEQLALCASAEEVVLALARRHELHAAVGTEPPGNAESRRIVCGIARQLGLLAGDVEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTF1</accession>
    <name>A0ABQ5RTF1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF289">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE VIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1048"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1592"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1733"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1901"/>
            <end position="2014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1734"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1903"/>
            <end position="1916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1925"/>
            <end position="1935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1962"/>
            <end position="2001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2002"/>
            <end position="2012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2133" mass="213398" checksum="6F3F2CBE0C7CEB04" modified="2025-10-08" version="1">MLLLCCFTGGTAVPQYLQAQQQDAHLENTPETTSITAGRGTPVAPLPSLIPSLGVVANGANDCLEEDRRSSPVTSRNLLKLPVGAPRFSELYARLSHIRGSWHARGVAAMHVLSQQLGLQIATLWALSSDGTYFVVQASGGTRRHALRPGASTPAALGDRQANSLQRVQASKMTTVYSCIEDGRNEGTPAAAAAGAPAGAPAGAPAGLVDPLNCHQYLGNSQGADRQAASIAASSPHSSRVAPAGMPFGSGGGAVTACSLGGGGSETTNPASTAMSRLPHDLAELAAATGGAFTDFAVLPLMSGGEVVGAVTLGLHRAQIPPQRRSGSRKDDASRRNSAPNSTVPVATTVTDSGIATTGGGGGAVVSAVAATFTGVQRSPSSLTDRIRRALTRDRDKDREDEDASGPISRAGSATAGGQKQSGCRVVMSATAPAAASSPPPLPSICWLHSAASVYQLSQLAHFLGYGFFSDTEQSSFVAQSCSLVSGVSAAPDVQNLVNCLIGTSKSLIYQRFRLQAQCLVALVHSSGTTAAFFKQQRTPRGSTARAGGVVGAATSTMTVQGRAQSQRDMASQPRPLTIGAAAGAAAAAGGGIGVSNPAVVASAGYCGPGTGGALPAAAALLQANLSNTSGGAGGGGAGGGGGGGGVSSSLHGLSAVLLGRKTRSQSQMRTTRSIPLQDPDNLMGYVSETLSTSVTATVISLSHTLLHDALHSQPPAGTAVRSCADTLARDGAPIRDISLACRLCDSRFGAGLGGALGEDSVNNSGAFAGHSASHAPPLSASPSMLRKSTCGRYAPLGSLVIALGLGFGAAAVGAGTGSTRVSGVGVRSSPDVGQSRGLMEAHPVSMGEETSFQNTMGGQVAGGIGTGVGGSGLGVGVNAGVGGGVMIGSTGNEPILALYVGFREVLQESVLERVLEEMTQVAELVAPSVQQRLLGSLLSEWAQMSSRLMHPSSFIGGQSVLSQGAGGGGSRRGRRRSSHEWTEALGTVELWAPSASNLSFALEDPAPLSHLQVLVNSLHSTLSSIQVERALEEGTDSPLLANDMRGLELLQQIGRGGQGVVYRGRMHGIHVAVKVIPTAEGGGGMGPVDRDVAFEPDNVEAARRAARRQRALLRDALEVAASAAVAHPHLVQLHTYFTDVMVVEYEGDEGRFRLMHVADTAPGDPTGAINLVLVLEYCDGGNLRQAMERGVFFRKDARLVASPASLPAARAEAAGAGGAAAALAAAAELHPNFEFIYLTLLEVALAIRHMHAMRLAHCDLKPSNVLLRSAPHDPRGFTCKLSDFGCVQLMRDVATTAVSSTGMEANAVNGATVMAGVQNNFAAAVSPATSTAAATSAAVGATSGTSAASGSSPHAAPGPACAAEGLRLGFLSGHVLGTPSHLAPEAFLPGQVLDGSVDIYSFGMLMWEVLVGIPALAGLSAARVHSYTSRGLRPQFPHNTPEHYRNLAHACWAQDPRKRPNASALVVALHKALMQVSPAAALEVRSMVMVHESGPVPYGAASVSPAPPSASNLHAYLHPHPHHPQHTPSLFGRSPVASDVPIGVAAATGASASALVSAAAAGHATSAVAVTGPLDAQHLIGGSAVVGSLGGSNGAAGSSQSCRQAFNRPSSLGRGQHRSVTNNTDASAFGSGGGCVAGAPSAAAAVSATAAGAAHMPQTLWPSRSFRRPQGSGGSARRRSLSAYHGIGGVISVTAAAAASVGVGVGSGGVPLPGSARSSIGGGSPLLLHLAQSRMSRSSRSPEPLSPGEEKNLSPGQQGADQHRHQSTFSGSPLIHMPFAAGRTEPAHGVPSYGGIMGAGAVAASVSGLPTARPGSNGSISLGGNGNGVNGGGYGGGGRGGDANGGGSGHATEVCLATAADMVDVALVAAGRIGSGTEGRGGSGAGMLLTRGSLSDFGIMHGGPSSVASGSSAGTQTGGGQAGAVGTSHPFQHSHLAHHHQQMSHQSLLPGARGGSAAPMAATNGATGSAAAGSRVSSRGSRNSRGSRGLGSSSRSMEGSTGSGNNGGGGSSAIAVRRIGRHTYGTAGATATHSPAAAPVVELVEGGSVPVHVSVCPQAGTSANSPRTAVSCQGARMAPIDYYGEGAGSESTFYTASNSIARSSSSAANTANTAVLTELLPPSRGQSAVGTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTI8</accession>
    <name>A0ABQ5RTI8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="260" mass="28291" checksum="354C541931DF8F79" modified="2025-10-08" version="1" fragment="single">MHEDAGSLGLAFCPPAVVLVNVPVQPDGVIRVTAKQLAAAVATVVTPAGVCMTASDPWVTAGHRLVRVIAYTAPAGIPHGDLGSYAYCEEQASIEGVPAPSWSDPRVTTTLQRPFAAGSSCLERRTCHVLQSGSTLVIADAATAKMQLYGTVSRIWALYGCLLSISPRDFSVPYDKGMVDTSVWNEFSCVGMWRSLPRDAKLERYSQLACRELAFFCYCHDLEFFRTAVLPGLRARLPSSRNCLDCWMAGVRGRELVETW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU09</accession>
    <name>A0ABQ5RU09_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003307</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47703">
        <property type="entry name" value="D-AMINOACID AMINOTRANSFERASE-LIKE PLP-DEPENDENT ENZYMES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47703:SF2">
        <property type="entry name" value="D-AMINOACID AMINOTRANSFERASE-LIKE PLP-DEPENDENT ENZYMES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="225" mass="24999" checksum="F28FB0B6EB28EA3F" modified="2025-10-08" version="1" fragment="single">EVSGADLNNPDGPQRSQAYVSIVVYCKAVQPPPYGPDHGVQAAVMGRPRSLPGAKASSWVEERRLYEERKPPEASEVLLSDADGRILEGLVTNFCVISTAASTTPSTTQEEEEEEEEMVLRVCGPQADAALPGVVQTRVVEAAQRLGLRVVPEPPLAVERHSWREAFISNCIKRIQPLRRVTCPPGNAWGADPWDYWLPYTAGRYTTALLRELELIEERIPWRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU61</accession>
    <name>A0ABQ5RU61_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chalcone-flavonone isomerase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003010</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the chalcone isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.890.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016087">
        <property type="entry name" value="Chalcone_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016088">
        <property type="entry name" value="Chalcone_isomerase_3-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016089">
        <property type="entry name" value="Chalcone_isomerase_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036298">
        <property type="entry name" value="Chalcone_isomerase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47698">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47698:SF2">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02431">
        <property type="entry name" value="Chalcone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54626">
        <property type="entry name" value="Chalcone isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chalcone isomerase" evidence="3">
        <location>
            <begin position="79"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="271" mass="29269" checksum="C9B1D3BAD11565A2" modified="2025-10-08" version="1">MVAFQTRDGYQVPEASKNPATVISHVLGLTAENFRFWSAKAAPFAGGPPVASLSSSILSGLGRPGTKGGREEPATGYEFPPEYCYLRTKNCPSLAGIGVRSKKIIIKDINVYALGIYVDIAAAKSALSSFKKKSAEELVRDQSFYNAVVSAGNVEKTLRLVISSRLVDQKKFLDALEERLAPRLKQAGEPGTLVEFKKQFDGVQFERGLEIAFTCPDNRKLVTKIGGQQKGVIASGALCSSLFDIYLGSDPVSKDAKDTFGRSLAASFHEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU91</accession>
    <name>A0ABQ5RU91_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013893">
        <property type="entry name" value="RNase_P_Rpp40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15396">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15396:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08584">
        <property type="entry name" value="Ribonuc_P_40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="437" mass="47074" checksum="DC9CDC62C9160B08" modified="2025-10-08" version="1" fragment="single">INIMPSKQGPRLYWRLSNLQHGNADLEKTVTEHLLNHAIQVQLPYDTVDKELQEELDVQLVTMLPPDEGYQVIRASLADILAQILNSAVILEEDATFQALSLANRLESNHAIAITPDGKLHMAIDSEIHARLGLTGLHSPSNKDRYNVSLDLRSKSLQPGQDGYAQVLAKVRSGIPPQAFIARLEVKGEMRELGLPPEALTPSYGTEAWMPLESTSRTLLGLMLPPLSRATFEQLGMDTETPVPGAGAGSSDDRDGVGGFMAEAAGAGGLHDSGGGGGGGGGRRLPLTAHQDLRLRGAITGLHTWLGALACGQGHTICNDLLPDEVMPLVLEDAPLPSRAEQERRLLGTLVSRMWKGMLSQRQVLNVMKATSELVSSQRVPWAAVHVWGFADTPVAWRGMEHGTSWGGAGESSYSVVFLGGGDYCIYRPLGPNEMRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU96</accession>
    <name>A0ABQ5RU96_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="625" mass="64257" checksum="E8CE4F67840D6230" modified="2025-10-08" version="1">MSQASPISSGGFVTRAERSPLRNLVGTQPAVGPGTYEVIRNPDANPAPVPFHTSSERALVENLTTSAVTPGPGAYGVQATGPGGNVVGGPASPFVSGVPRLPTDPDTRARIPVPGPGTYEIGNQWVKGGHRYTPQGNKAAVRVSQLLRRRPTAPSVPGRGESYGYEEESDGTLVLQSAPEKAYAGVGRDTVGPGAYELGGGGPFARPTGTMWSNSKSGRVYQYGPTTPGVGTYNLADSGSRRHGAGLLVTIGGVEVVFGGATGTSSFVSKAARPLQKLPETSPGPGDYNNLVTPTAAPLSGLSGGALRSISPGFGATGPRGIWEVDPTKAKSAPTYWRNPGPGAYEDPRVRRPKSLAADGDASAVTSIIGAATPFKSTSLRFGSADNTVPGPGEYHPDAVTSLEYDIFKKVTISRPSGAFGASTGRFSYNKGAASPVRLVVPGVLASGEALDAGNPGPGNYDPKSSPSGKTARSGSSNFASRTGRFRPVTAPPAVPDLSDFDGTNSLKGDPTRLGPGSYNPERPSGRPRYNNTPAKVSPFGTDSNRVNMELPSKATPGPGRYPSTDPQAFKPTTIPPPKTGFSSQADRFGASVTFTPGPGTYKAGSDAAFVRKSFNVTYAGTVHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUA2</accession>
    <name>A0ABQ5RUA2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="510" mass="52735" checksum="77C0A55EC357A740" modified="2025-10-08" version="1">MGSQLHHAQAFYRFSIIASILAFALAGSFARELPYRSGRALLGGQGSLRDGKVAGSAPPKPREVNETHSEGSDTAPGNVARPPSGGKGHGRGLLSSKGAEHGQGSAPPKPQQVNETHSERNATFPKPAEVNGTRPERNDTAPGYVARPPSSDKGAEHGQGSAPPKPREVNETHSEGNDRAPGNVARPPSSGKGAEHGQGSAPPKPREVNETHSERNATFPKPAEVNGTRPERNDTAPSNVARPPSSGKGHGRGLLSSKGAEHGQGSAPPKPQEVNETHSERNATFPKPAEVNGTRPERNDTAPGYVARPPSSDKGAEHGQGSAPPKPREVNETHSEGNDTAPGNVARPPSSGKGHGRDLLSSKGAEHGQGSAPPKPLEVNETHSQRNATFPKPAEVNGTHPERNDTAPGNVARPPSSGKVHGRDLLSSKGAEHGQGSAPPKPLEVNETHSERNGTAPGNAARSPSSGKSAEHVQGSAPPNPEEVNCSRQPSAGQQVSGKRRLGDVAGPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUC0</accession>
    <name>A0ABQ5RUC0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="4">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003038</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF17">
        <property type="entry name" value="PHOSPHATASE IMPL1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="344" mass="36776" checksum="9BF89FD6B2CFBE24" modified="2025-10-08" version="1">MMLCKGVFGKSSHAPRCYPDGHRLPAAAAIARRGRDRLVVLASKVPVDQLLVVAKKAALLGAEVVMTALDKPRSISRKEGTDIVTETDKASEAAVVGALREAFPDHAVLAEEGGVLGDVKSDYLWCVDPLDGTVNFAHGYPSFCVSVGVVRHATPVAGCVVEFLPTQGGNAGSAGWTTRTFTAARNAGAFLDGKQIFVSRVKELRDALVATELVYYEDMWPQLSVLHRQFTAKCRGVRMSGAAAANLCHLAMGCIDSYWQYNLKPWDVAAGLIILEEAGGRATTGDGLAYSVFDRSLIATNDALYEKVLAVTEPQTSAMLSEGVSLSPWCVPKGYRVRSGAQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUT4</accession>
    <name>A0ABQ5RUT4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="14422" checksum="655EF64D71501211" modified="2025-10-08" version="1">MNGTAIGWKSRLQPTVATSSVEAEYMAAASTTREALWIRKLLNDLGMEQDYVRIWDDNQGAMSLITNPISTERSKHIDVIHHFVRERAERGEVKLDYCPTESMVADMMTKPLGDKKFAEFRTAMGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV69</accession>
    <name>A0ABQ5RV69_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GCVT N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_003790</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GCV_T_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045179">
        <property type="entry name" value="YgfZ/GcvT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602:SF0">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="GCVT N-terminal" evidence="6">
        <location>
            <begin position="170"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="521"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="569" mass="59724" checksum="A3FF57955603496D" modified="2025-10-08" version="1">MSPFPGSNQRSLAVGPRPFTVSRVPVSTSCRGGVHHGRRVLVSRRRGRSGSSAAATSGATGSPEVASSRPPNRPSGARHADTGCLKQGCSPPVQWRGGHHHHYHHHHQRHNASSCGGLVVTAAASGPLSLGDLLLDVPEIDGDIRSLQVEMGAIFNDAGLATTYGRKREALQALETGVVLVDQSHWGRLRVTGEGRLALMHSQSTADFHRLQPGQSADTVFVSATGRCLDLVSALVLPSSVLLLVDSREGKDALGARLDKIIFRGDNVTVQDISTRTAQIALLGPEAEVVLRELAPDALSGVLGAKPGTHVLVGFRGKPVFVAAMSGLGPSVPGYTFVADETVGGDLLAALAAKGAIPMGTDDWEAARILVGRPTRGSELTDAYNPLEAGLYAAVSLNKGCYIGQETLAKLHLRDGVNRQLWGLRLDGPTSPGAEITSEMSKVGLVTSTCQDADGEWVGLGYLRSRLEGTQVDLEGVRVAVAGAPATVTAIPFASRKFSAEAESPVVRAADAAGAAAEADVDSIAGRLEEAKKKKAEAQAEKQAAMESKLKAMQERLTAWQAAQQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVB2</accession>
    <name>A0ABQ5RVB2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003437</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="387"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="491"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="615"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="727"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="766"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="851"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1424"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1485"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1542"/>
            <end position="1563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1922"/>
            <end position="1969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="623"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="634"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="781"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="854"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="933"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="965"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1506"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1936"/>
            <end position="1949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1954"/>
            <end position="1963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1969" mass="206314" checksum="C87B9396C148BD04" modified="2025-10-08" version="1">MAVIAPFLQRQLYDEAATEMADELRHKVDLLACSDSDISDNDAKGTARPHWELPKGANVAATDRADEVLVRKRTNMSALRHRNRPGTLTGPDKVSNWLLGSKDWSRQFLRSHGGISSPIIRKSRRSTGAVSSVLGFERSYSGGPTRHVVPYEEFNNWWLRFNLRGRLEDAFPVLAPNTHGRTSWDIFVLTLVLYSAVTVPLAFSYGLPSSPALNAVEWVLTFVYIVDIGINFRTAYHDDDGNLVRDSWPVAHRYLSTWFPIDFLATVPFDFIGLAAGLGDSESQLIILAVLKTPRLLRLVKLMRLLDRVRNANLFKVLQLILLMMMIAHWLACAWYVIARYAPGSNDWGFDTLDDENRLTWYLSAFYYSFLLLIGDNIQAMNNYERLFFILALVAGACFYSAVVGNMALLVANMNTVAVRFRQKLDMTQDALRYMRVPEAQRERVQAFYDFITQFSHPGSEGLGFLSELTKGINEDLHIFLHSGNLMRIYLFKDCEPAFIRSLAMRMKIVVFIPGEVIFRQGDLGHEMYIIRAGCVAVVSKNSEVMSLLQAGDFFGEIALLTQSRRTAKCIALSNCDLAILNSFDIKILMKEFPKSAHSLQEAARDRLRQLQIAGRAGKGSSYDDDEGDGDEDPDKRQEREEVRRQRRSSITRFTTGRGVYSHEVSPAPSDGRTGGSSCCIDGTPQVSPLPPLAAAPRQDQVAWTTAAPIASAGGSATAEKQIGDSTAGAAAATTEPSDGLDSNLLPASYVESSRNSCASLATEDSAREFASRRRTNSVTSQGGPSNGASASNSRCNSGAGATAAATVAGAAGAPLHNTAANGDVRLQCSGGCSEPQSGATAVGLCDGGGGSGEPAAAAVATVSSTATPQVRPPMNKIRRRSSTAEGVRQNHPNGGSCGGGQGATSNPNLSEPAAVAAAPRPQRPPSPHVRNPTPSSSSWSALFTRRLEPRPGTPGVFCRRARSRSSTSGEGTATSVEGQPTVLRVTSAAMESPAPAVGASVTISSTSDGTYLDATTRFDAVERFHPPGPMSPVVLPTSAVSPVAVQDAVPTAKKFRASVLLFGRAIGVGPLAEAVEQSAPLYMKQAATLHTKLRAAAAPSSRDGSSSGMAAAACLTTAVSRDPSALQPMGNSRRTSIASIVESRPQVLSPGIKAAPGRREHRSSLILPYSASSTTEFAAAVAAAMGITSPLLDGGGGSGGDGRGRVAAGAFVNALAATAASVAGHGAGTGSVRRGSTEIEGSRVVQGLITSHDGEAQSVPQIHRHGSSTLCVPLRTWYTPPSPSDGVATATAAFMEATALRTPEGAALSSHLNAQGPCSLMRSAAARRADAIHSPFESPFSLRGMAVEGQGAGVQSPPFRLSPSATVVNLAGATNGKLQADRATAFEDGNAFSYSALEGTTRNLRALGSMELLQLAPPGYEETLAEGAGDGTGTGCLRSSGSGTGPCPSADRLVRTRQIRNVRRSLQSGAGTLPEAFSTTAAAAAAAGNMTPEEMEAGPRRDNDGGAAAAAAPAVVASEDGGDEGLTPVMEWISTARATTAKARRQRRQSSVGFFSPQPQLRGPLPGAVNGQSPFAGAHVRAANQLAELQCAEAEHMRREAALARRESDLEHREAELALRHAEAVAALAEATQRREEAAAAMRRTRSVHVMVVALASACADLAFKVEQLDLRLEDTTAAVGRIEAQHGHGRPHGNEFLGLGQSAVTALPARDTRPGVLERNASIDKRFAALERTKALSLWQSSGGGGGGGGGGGSMRHGNSLLHGAGEIATGSGDVTVSGRRLAIPTSSGISGVESKEAAAAAAATAAAAAASSKIRRSSALRADVFTTTAPSPPPSGFVSRGAISFSMVPTSAGQVRDSTSPSPSAAVRGTMAESFTAGMQVAVPAAAVAVAGAAAPASTNPLMLFSAALGNSFRKARQESPWSQHASSGAVPATGLTTSASAASGAVHTEVAQQPITVKSSEIEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVG6</accession>
    <name>A0ABQ5RVG6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF444">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="376"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="470" mass="51260" checksum="E94A08A322B4D97B" modified="2025-10-08" version="1">MSEQLAVYRRCRRQYPGTAACVLLPAFCRRKAEASLRGMRCVHRFELGTILFNGVDREGMLAPWPGTSYSVDVWYEAPDEGAVGYGPALSALTLSAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVAEVNALGLTPESSDCPQVTLADGGKHAIVGRITLSLSIGPLRVVTRPYMLQGLAETAPYIMGSSTLRQYRARIDMETATLHMCKGTLSIKVPFLSFEHVTPKEGEAEAVPMVNFAVTAMAARIKPELVGHKEAVRLLRKGAHGLLLRPRVGCCATTTQISVDPEVEALLSQYEDVFKDIPGLPPMRPVDHTIPLMPGAQPTARPMYRLSPLELDEVKCQVTDLLAKGMIRPSTSPYSAPILFVGKKDGTLRMCIDYRGLNASTVKNRYPLPRVDDLLDKLKGSAYFSSIDLQQGYNQIRIAASDIPKTAFWTPFGHFEYAVLSFGLTNAPATFQAVMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVJ4</accession>
    <name>A0ABQ5RVJ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_003845</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PNO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22391">
        <property type="entry name" value="KH-I_PNO1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22392">
        <property type="entry name" value="KH-I_PNO1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055212">
        <property type="entry name" value="KH-I_PNO1_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="147"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="231" mass="26034" checksum="C649824291B08F3B" modified="2025-10-08" version="1">MPKVDRNTRKQPLKEVGPVDMEAEDAGAAVLPEKPKFAPLSRIDEKAGGDLKVEWRRVPVPQHRMKPLKASWMELYTPITQNLKLDMRMNLQTKKVEIKTTPNTISPDALQKAADFVHAYLLGFEIRDAIALLRLDDLYVECFEIKDVKTLRGEHLSRCIGRLAGKDGKTKFTIENATRTRIVLADTRIHILGSFQNIRVARDALCSLILGSPASKVYTKLRSTCARLNDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVN9</accession>
    <name>A0ABQ5RVN9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Reverse transcriptase RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004071</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34072">
        <property type="entry name" value="ENZYMATIC POLYPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34072:SF41">
        <property type="entry name" value="REVERSE TRANSCRIPTASE_RETROTRANSPOSON-DERIVED PROTEIN RNASE H-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="7">
        <location>
            <begin position="35"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="332" mass="37646" checksum="76415B4C1033FD4E" modified="2025-10-08" version="1">MSDDAFPDRAVAQAITKWDKSCEKAFQVLKDTPTSAPFMLTTDASDYATGGVLSQVQNGVERVIAYESRKLTPAERNYPAHDREMLAVVHCLRVWWHHLKGPHPVTIWTDNSAVSAFKTQPRLNRRQVRWLADLEEFNYVIEHKPGKTNVVADAVSRRPDLRAHPMTVSVAKAAIDLATTIRRDGTQDETYTRLKEQAIKNSKEDPPFNLYKSLLYYEPRGALRARLYIGGYVHHHLPLVPTVQTQARPPPGQISTTAHPRAALAVHFPGSNPGPAKIAPWRRRHRRLCMPAFQDDARHPCQHEHHSRAPSRGRFRLGVPVLRSAREQCNRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVP8</accession>
    <name>A0ABQ5RVP8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_003704</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005747">
        <property type="entry name" value="MutS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01069">
        <property type="entry name" value="mutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48466:SF2">
        <property type="entry name" value="OS10G0509000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48466">
        <property type="entry name" value="OS10G0509000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="5">
        <location>
            <begin position="654"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="210"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1091"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="242"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1133" mass="120713" checksum="FC4AC936975774C9" modified="2025-10-08" version="1">MTRNLGPTSRIFESQSNTVIIHHSIRIFSSCMHQRRHFGLGVRSLTNHRKTDGWLSPFRWLCPCASLSGSSSLVINSGNFDKSPDDGDDTGLAASAEAESQQILEWPVICKQVACFCGTSIAADVVARGQLPRGRSLAESELLLQQTAEAMQANLEVQGCFDIRPAVDAAAAGRCLNAKQLEGVAATLEAAFVLKGAATVVDELIEDGMSGTHTCRGGSSSMSHRGSGHSDLDISSEPDCASPSSSSPSSPSSPSSSSSSSSSSSSSSSSSLYSQPSVAPLCPLSRPRFPSLAALAEGIRDEERTLLRAIRTCIRLNSVCNEASEALAAVRAERQSNKERLRQEVESWARLMQQRGAAETGAVSLVRGRFCVGVRAGRQGELPRGSVRLSSSSTGATLYIEPQPCVELNNTEALLAEREETEVQRVLALLSKLLASRASQVLELLDSVTVLDVVAARAKHGRWLGGIRPTFDEEPGASPLYLPGALHPVLMQRGLQPLPQPPSVDDNRFDRDFQAAPAWELRRVLVPDGPRPGQLPDGNTAATADDEVRRSLLPRPLDLRVPPGKAVVAITGPNTGGKTVSLKAAGLMVLMAQAGLFLPCDSTQIATHGSPRLAWFDRVLADIGDAQSLQQNLSTFSGHIRRIRRILATAGSRSLVLLDEVGSGTDPLEGAALARAVLDRLAGLAYLTLATTHHAELKRAVDEDGRYVNASMAFDTATLSPTYQLVWGAAGASNALDIAEALGFDRSVVLEARNVAQALAASTSTSAAAAAVGINDDDSDGESAPSGSEGESHIAVVARSLVRQLDETRQELEGQRVLRQRREAAQARLQETIMQVREMEQQLKLSPREIVMERDSLAADVQSALDAFALGLQPQATVEETLSRIEALIPEEVAAYRGQSYPGGVDDEHDPGAERTALRQGDLVLVKSYGDMGSAVVVSLKGDYVTVKFDILPLSIGARQGHKRKFHKREVQRVSQGSAVPMLGSSAWQSGYSNLDSDIKRVLGELSAEERQAAAEAAARLRAEQLAEEAGHASYNDLYGALDVDEVAADLDNVIDDPDLASLVDSLGQEDENNSDGDTMYRHLCDTPGGIVQQQQAATSKRRAGANAEKSSSSSRRGAVGTGGELKLPPEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW85</accession>
    <name>A0ABQ5RW85_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004290</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021183">
        <property type="entry name" value="NatA_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF2">
        <property type="entry name" value="N(ALPHA)-ACETYLTRANSFERASE 15_16, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12569">
        <property type="entry name" value="NatA_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000422">
        <property type="entry name" value="N-terminal-AcTrfase-A_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="609"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="631"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="911" mass="100300" checksum="DD14CDEF493944CB" modified="2025-10-08" version="1">MASGGAQPNQPLPSKEAGLFKQVLRFHESKQYKKAIKTADQILKKFPEHGETLAMKGLCLRNLAPDTDKEKKEEAYELVRKGVKCDLKSHVCWHVYGLLYRQDREYKEAIKCYLNALRIDKENIQILRDLALLQIQMRDLQGFVDTRHQLLTLKPSNRSHWITFAVAHHINGNHELAVQVLDQFERTLDEVPASEAYEHSEMLMYAATVLAEGGKPEEALAYLEKRKEKIKDRLGLAEMQAGLYLRVGRKAEAAAAYRRLLAVNPNNYKLHDGLRAALGLAPTAGSGGVLTDEQREALSALYNELQEQFPGSSACRRIPLDFKVSEAFKGAADAYLRVGLTRGVPSLYVDVRPLLSDLEKAKTIQQLVESYRDSLRRMGTYPPLIGAANDTLQVPESPQTLVWVLLFIARLYDREGRLEEALACIHDALAHTPTCIELYVAKAKILKHAGDLEGAAHVAEVSRRMDLQDRFLNSVAVKALLAAGHCKAAERTAALFTRDGEQGAYSLYDMQHMWYEVAAGRAHAARGPHAAGAALKKFTAVVQHFADIHEDQFDFHSYCVRKGTLRSYVSMLGMMDRLYGHDFYSKAAAGAIRVYLKLYDCPPGAANGTDEETLLASMTPEDRKKYKLAKKKEEKERARKAAEEKEREEREKREREKAAGQKGGKKANTASSKREPDPDPDGAKLAATADPLGEAAKLVEMLVRHAGDRLANHLLAAEVALRKGRLVVALGAVRKAAQVAEGGAAHPDVHVLVVRLALAVHSSPPEHPVVRTVVAAGLTTLLGDSSPAQYAEAWRITHGTTSLAHRVAAGNAMVLLGAAGGAGEQQAATTAAVAHVTAEDLSSSPHDHVQCVEVHKLLRDTWGDATAAERWRTACAAAFPHSRYFGGAKLMALDPTYDFENMREAFGKLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWG7</accession>
    <name>A0ABQ5RWG7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004391</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF62">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN 60E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="521"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="657"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="703"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="734"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3042"/>
            <end position="3060"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3072"/>
            <end position="3096"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3116"/>
            <end position="3137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3158"/>
            <end position="3186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3290"/>
            <end position="3313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3402"/>
            <end position="3420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3440"/>
            <end position="3467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3488"/>
            <end position="3516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3536"/>
            <end position="3558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3578"/>
            <end position="3605"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="88"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="529"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="3041"/>
            <end position="3319"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="3370"/>
            <end position="3610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="437"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="767"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1095"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1132"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1238"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1316"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1457"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1626"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1712"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2000"/>
            <end position="2026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2158"/>
            <end position="2180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2278"/>
            <end position="2302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2353"/>
            <end position="2393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2495"/>
            <end position="2697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2711"/>
            <end position="2774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2812"/>
            <end position="2841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2946"/>
            <end position="2972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="437"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="767"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1253"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1325"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1354"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1490"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1528"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1712"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="2015"/>
            <end position="2025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="2278"/>
            <end position="2288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2353"/>
            <end position="2371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2377"/>
            <end position="2393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2495"/>
            <end position="2517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2567"/>
            <end position="2579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="2583"/>
            <end position="2595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="2626"/>
            <end position="2640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="2660"/>
            <end position="2670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2682"/>
            <end position="2695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2758"/>
            <end position="2770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="2960"/>
            <end position="2972"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3943" mass="415334" checksum="745730658F254BE4" modified="2025-10-08" version="1">MSFSDGREQLHALRLHLEGKQANGDTPAVVAARTGCAAVLTTIHSGILSARDKAVQFYKVRLDSNDRSWWLFTQQHVLRVVCLRVTVSRWFGRAVLTLVLASCLVMALDDPGCTDACKQEAVLNKVVSERCRRCEMYRQASTVLDVFFLASFTLEITIKSLAHNFVLGPGAYLKSGWNWIDFLATASGYLRYLPMGDVSGVSGVRAMRALRPLRALNAIPGLRLLVETLLEALPLLLDVIVLLAWIFFVFGIVALNLFMGKLHKRCSMMVDPGEGGSEGLPLQPPPSPGASWLNSSTLSNGGGNGSMNGTLQPQQQDDHLVWQVVLGLENTPCASGGAGLFKCPDNSTCIDINSNPDFGYTSFDNFGAAAFCIFQMLTLDGWTGTLLYPLMNAMGPYVPLPYFLLLVLFGAFFAMQLLVAILSSKFAQLSAQLPKKKKERRRRRMHTPPDTTDEEDEEDQPPGGGAPSGFNEEGEPLPRRRLKRPPLMTRIRRRCRRIWFRFRKRYLQALRRKDLSRWRALFWDVAYSSWFNKFMIAVILSNTITLGMEHDGMSPEFATGLEIANLVMTSAFLLEFVVKHLGLGLVGYWRNPWNLLDGAIVVSSAVELGLTYGPEAATPTNTQALSAFRMLRILRSLRLLARVEDLRKLLRMVLKGFVVLKDFMLLLVLFIFIFSILGLQQFGGSPYFAPSPNDVGFSSNFDTLWQSAYTVFQLLTADNWVTTSWDGMRARGTAAALYFVAWIIVGNFVLLTLFLAILITNFQGDEDPPPSEDELSGAEHNPFAPDDQSETASTLGGTLLGDVGGLKDQRNALQQRIARCTDPAAVYRLKKWLILVGYHHGMMEHEKEEITHDMDMLYAMNPCVFAPGAVPSVLDPEDLFMMEDDDGGDSDSRPPSANANVTVPAAYGAGSPVGGSLSEAAAAARAGLKRASVSLRMTLARLGGGSGAIPTSYFHSQIPLSSMAEEDGNLDTAGSWGVGLGSTDASAPANVIMGSGGIVGVGGVYRAGKRASVGGMTSAAVLGAGKSLKKLFVGGFNALGGGGGVGGVGAGVGGGGGRGGLGPGLATWGGAGTGGGGGRPNREQRLLGRNSAPLAAAADGGEGSGAGTEGEATSQATGRDAARLTENGISLWNTSGQRENGTMDRVAGPGGPSRNLSRHRSAVVRRGDTATGITGGNTPGRPTLITVKPQRPMLPENVAAAIAAMRNSAAWGDVAHRLSDTGIISTLATPQLEEAAAALARGHGKPQPGEEGGSSEDEEEEDEEEDEDRNVPSPAKRQEMFVERWRSWFDDADNAPGSGLTSSYGATRTHSIELLPSMGTPAATGDGGGGGGGGGDGGRDGRRDGGGCSGIDEGGGRTRTLRDGETCSGLEDVAAVRQGEGTREAKEAGKMTIRQQQPVMDGVVHVQQARAGPPLVDVDTDTTVAPAREGSRRRPLRHVVIPAVAAVVSVAVAPPVSEAPATPNMGFEASRHHSSRRALRRASLIRNTASRPSQSSNRSHSSIHSDEGEETGGRGGAANGGAADNGGDSVGGDKGCGEGGEGPEAFLAGMATAVTDSGKLGGLPRCNRHRRSVCLVDAFTQVTSPPDTATFNILVSTAGAAVPSPGPNSATIATTAADADAAAAATEARASGVHPQRRSVDGPVPSLLQNTRNRRAAEQEAAPPRMASWRSSPRPQRLSPVPGLQSVRAATSSQIVPAPFQLGANSLGSAYISSSTSSAGPSANLRAATQQPRPVSAAAEPEGRHVRDRGRLQAPRGRWLEATGQAGEASVANGTAKNTAAAGAVAGYAAGAINEERNIFATVRPAARAELCLAERLARPLVPVPIATSSFRLAGNGDGGLGGAGDQQGYQHNYRLEAGLVHVEDHEKQQSPQHRDNIHRAPGSVALFLGQDSLPSTSQCFLHLHPQPHPHASRTSSGLVDLGRPSSDAELAMFRLRQVTSSGGAAESSCSGLGHYPGSSGVHWQQRIQLHDHSSSGLHRSNALADGFEDEAKPRPNLLIEQQQQQQQQQGHYGHVGDTEARGDDDGVGVGMAMEPGPITWLSDSSNSGIVMGDVGEGSRSLTPGALSSGATRWQQQRRAGHDTLTALRLAPAAAAVEDFQVMELQKVSRVDTAFGRYATPDGAFGRVPELPDAAPGDPPPQVGWSIAVSPAATGVRGSTVAKKPMHASSWRQVPGRMTPPPIFSVSEESVDEGSGGEGGFVLPTTLSAPRRWEAQSERHLLRSFRSLDWGEGGAAVAPAPAAQQQQQQQPLLLPLAGRHLRELAAMQVARLDGGGGIGGAGGTGGGSQDTAADGESGPSPTLASLIAAASVAADIGSGEQSVGSHSSFAFTSVGYLDSAALAPTAIPPPYSAHLLSSSSPSRSRMRIYPPQQQPPRSGSSLRIRSSTPAPSLPLQLSAQAQEARWEMDGAIELQEPAGSTFAFPTSIHAVTAAVAVAAAATVDPRAAPAAAVSTATKLNPFGIPTATHLDRTTSRRYAAAAIVFSDSGAIPAATSGDSSITASASASGSASASPASMIPRGPHPDSLRSPVRGLSTAAMPLTAGRVRERSGCNSGGANPGIGAPMAGSGARAGLGRRLPFPPRHETPEERELHHTPPSLYALSRPQSPSLGGASAQGPKMRQLGHCGGGGGGGGGGGSAGTFSRIPLPPSAAPSKMQEDQGEGEEDEDWLPMPPLPLAMASSTASQLPSRQTAADSGCNFADPAAVMASRMGRGNDGTTLQSRLAAGGRGRPVAESTGWKPRPDSQQGVDGRDGEQVQWSKVRTGSEASSRGGESWWTPVIVAASGSETVWEPQALTAVPARLLYDYQGRRSRSRSSSSGGDGDGFSGSTSASRGHGDSDCYDRSGTAGCMDHARVIQGQYTVRSGLRRRGGNQGTFPLRSTAAAAVTESRNLYDVGGDSSVGGRGAVRHDIHVTAGDDVIGGGGGEGQSAAHLGVVELQLDEDIEGGDSPAREAKTRNGGGSVDAAGVGGPLRSVSLTGGELAGSAERQPQAKSAVQVKREAMVHRRRNEQVYLNYSSFFLLGPHHPLRMRLQFLVTCKWFEGVILVMILASCVVLALDRPRNDPSSRVAHVIHYLELALNVLFGVEMCLKLLLKGFLFHPGAYWRNPWDVLDGLIVGASLLAMVLSSVAFFRNLRLLRVLRPLRMVSRVKMMRTVLETLIRSLPAVANVVLFGGFLFGVFGILGTQLFSGRLSRCNQAILPDGSPVHDKSQCVPGTFICGPADVCLDLDAPTERQWLTPFLNFDHIGRSLLTLFIVATQDGYMDVLQQVIDAVGRNKQPRYNHAPWMGLYVIIFVFLGSFWLNLLVSVIIDYYSRLMLEEGDLLDSKEAREWMKLLQFSGRTQRDYWRKIPVPANRIRARCYAVASYPYFDNFILGVILANVVVMAMPHANSTPRFDDAMSYLNAAFTFIFIFEAAIKIAAMGPKLYIQDHWNKLDMFIILTSIPDLLSMIVPLGAGTGVITVLRIMRIGRMFKLIKNARGLRTLFNTLISSAPAMFKVGSLLFLLMFIYAVLGMNLFGAPGSPFDDYGDTNFDSFGASLIALFQVFTADGWSAVMAQAAGCDPMQFQCRTGGQALVACLFFCSFIMFATFIMLNLVIAVILDNFISNAQNEGLLKTSNFTDLMKMVIALRVFVRMLRVKIDGMRMIDELKAMTAAVTAAGGDSHGKDRDRRSRVRKKLIRLNGGSEHCPTPVTGALGSCPSLILGDGLTSRAMSVQRSSYQSVMSRLLLGGGTGTAGGGAGGDALAGEYRDSVRSRSHIAGAGGGGLGFSLGFNLGSLRRTFTRQCSSGSVARAVSLSGPLPHLTGATAVAGAAAGPRTRQQVGGPMLSGPGAHYEYEAARTVSGWSLWQGAGGDGPEGEYIGGGPTPDPADIPGEVAVGFDPGSTLTTNGEVAAAAATAAAAAASVGASGSGAVSQSDGSTLRIRNRCNDGLGAERPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX85</accession>
    <name>A0ABQ5RX85_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">LOV domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_004482</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF5">
        <property type="entry name" value="HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0157">Chromophore</keyword>
    <keyword evidence="2 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="1" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="691"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="757"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="833"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="1611"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="350"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="397"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="450"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="527"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="606"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1119"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1184"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1243"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1333"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="451"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="527"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="606"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1122"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1184"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1351"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="1663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1753" mass="186838" checksum="B4E5C9BB2B11D432" modified="2025-10-08" version="1">MAGATCADKAPIAEDGYRCYRLSGTVKNFLRCSPQPLCVIGVPTSRTTASYGSLETLGPGCGSGAASPSGGGTIPEASCGLVSSSSSRISSDGGGLVPTGPYGWAAYAVLPGGRVLPGANNTGARVATAGSVEGSMASVEAAPGDMNLHNDVLQAEVLYSNDALTEMCGFSHQELRRSGLALLLRPSSPVGAARNPRQGLELLGAALASGSASRLELPCCHSSGRLVWCNLSLSPVDSRPCEGADKGMIAGTLTSAGTAPASASAISMKGGWKRKVSLGHCTGRVDLSATIGCCGAGHGVGGRDSSSSFSSSGDSCGPDLGRAGRDCRNPRGCSLAKRNGCECCTGSGWPHEQSQRRHIHRNVESHPLQQQRKGGTKQPQRDKEQLELQCHNICQQQVQQSQPHQERQPDTGDSWQQRQRQRQRQRQRHQHWREGAEELEQDWEGCVVREEWEDNEGEDEDEGGPRLGEDRPAMRKQSHEWARQCQDEERVRRLRLWQMHQLEEQMQPLVAEEEEQYEEQGTVRLHHHHYHQHHQRHQTHQHHQQQQLQEQQLMQNTQQLQQQQSAILGEQQQQQQQQQKQRQQQQQLYLIIFNDITSHKLLQQELQQQGREQQQMPQTSGCEGHALPRPSRITGGIAAAPLPTPAPVAVDSDVPGLDLDCPPRCSSLVSRLAAGLTLQSCASCRLHDLTLDSLREGITIADASMPDYPIVYTNKAFLRMTGYSREEVLGRNCRFLQGADTNPSAVQSIREALSRRQSVTMQLLNYTKSGVAFWNELRLEPVLAPGSGQLLGYLGVQNDVTELLARKQSEAQLRDAKEAAESAAEAKSQFLANMSHEIRTPLNGMIATAQLLLGSQLNPEQRELTETILESGSTLLGVLGDILDFSKIDHGSLELQRRPMCLRQATEACMDLVAAEAAKKGLALAYLMDDAAIARPLLGDPVRIRQVLANLLSNAVKFTERGEVVVRVFVERRGETPRAITSAPDPVLATMALPLAPAVVVPSGSWRATATQLLPPAAAGATSTTACADGENDYTAIHIAVSDTGIGIDRESAGKLFQHFRQGTETMSRRYGGTGLGLAISKRLAQLMAGDIWVESELGVGSTFHFTLRGEWASVEGSWPCSPSQSSASTAANSSDCTPRSSTEEGHNSAGGGAAAAAAALGLNSAGAGRWSGSPSCGHAARLSSSSCGSCGSSRGGRELRTSPWKPDGTPAGTSEKNISGDPTAIKAAITTTAVTSICSDDAPASPGLPGPTHGSSVGCDSGSSHAGTAAAAATEVGEERSALVGRKVLIDISHVATSTQVWNSCRQLGLAAVQANACTALLPLPLPPLPPPPRSELPMAPACDDGATSPMPSSVAASNGVAATSPGQRVSQPMYDILLVSMDRLVPALKAGWKGRPVVALGNRELLPPTLQPLVIALAVPVRHGRLATAMVKATALLRWNGSNAPKLGNTPIPSESIQTLKSWRCKRGPDWEGVNRRTSLDNSALERTNLLLRGMRPAGLATAGCVPGDVVGGGPGQSGVGSGKMSPGRLQHAPIPEHAELSTADDAHSDRFCFRASTQAPRQITPPETPSVAGSSAAPAVAVSPLGLPAASGPISGNIGLDMMQQQLRILIAEDNKVNQKVVLKVLQQILGGCQPPDVVENGLQVLQALQKKTYDLILMDIHMPEMDGLEASKRIQETYKPEERPRIIALSADTVQTLHDRCREAGIEAFLVKPFRIEELARVMRTGAQGPRSTKPMDQDAPVCGAPFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXA0</accession>
    <name>A0ABQ5RXA0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">indole-3-glycerol-phosphate synthase</fullName>
            <ecNumber evidence="3">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_004496</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854:SF2">
        <property type="entry name" value="INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="9">
        <location>
            <begin position="65"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="331" mass="34913" checksum="F8139FEDF035DAC6" modified="2025-10-08" version="1">MPSAAVPSASTSYNTSNSLPVLRRWRSYFLSARRVPAVLRASGSPNPTGPDGEPPKTPMNALEATIRRKQRELETTIRELGMEALDERLQSATQMPLNPPYRLSTLIAESVPQGRAVLVFEVARSGSETSTADLAELAKAYVTLGGASALVVRTDSDATPSGLRDLFTVQQAVPRVPVLARDWLIHPLQICEVKESGAAGALGIINQVTGRGTGVMSSFSAALGLDAPVEVVNAREVEQLAKLGVVFYAINVAVGLSVAMPGFSNRIAHGLLGELPFGAISLVGVRSLEEAAAARRSGADALLVKAELLQSYGKDVQALGDALQYAVTLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXE3</accession>
    <name>A0ABQ5RXE3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019138">
        <property type="entry name" value="De-etiolated_protein_1_Det1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13374:SF3">
        <property type="entry name" value="DET1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13374">
        <property type="entry name" value="DET1 HOMOLOG DE-ETIOLATED-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09737">
        <property type="entry name" value="Det1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="473"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="587"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="933" mass="99657" checksum="CB4088AF078F6DC6" modified="2025-10-08" version="1">MSKNNLNACKAIMARELGGRRPGTATSQCRRLYQSILPTNKVQQMEVPEAHICRFSPCGNYLVCLGGGFTQLVVYHLTGPRVSWQGELSEAEIASRLTSFSSFFSLLYRVPLAPVREVIARDVVLFMAGGSLLLLVSHSPFATPAAQPSVVPDADPLQSLPFAESITLHLVRLSDGMRVGGSRLDCELVDLTGRPGNLGISVHEDLIAVLALRSQVVHLLQVLRGGEEVVPVRSLGPYVAEDDELVLRIAAEAEERWLRARRAEREWEEEASACAALPPPLRRCYNGDLVRTTDVDGGQPSTVAAASSSGATRHQSTRPLAAEAGGQIGGLARWSRGPSAQPPRTGSVGPGLDLPLEPGSGEGAGMVSRQTVTSSGGEVLQGVSFRGTMPGAAAHAASDAAVSGVDAGGRVGRAGSGSGSSAVLAPSPVSILHPPERFQPGQGATAGVAAAYGPGSHAAPAADLAAAAAQQTIMRRQMRQQQYEQLAAQGGPGHPPQARRNVPQGGAVRVASNPNQTGQDAAGFVSAPQAPHHNHHQQSNNPPPPLQQQQQQFPVPPRQGHYHHHNRHQHQQQYSGAYPQTAPRQIAVRQQQQAPGPAGPGRVLLPPDGGEGGRGGSNVSGGCILGIKQRLLAHLYMNCRKSYLNNPRRQQREAIALFRHLDTYRSLLMRKVYLLDRSRLLIDMRKPMAAELGQSVFGGGGAHSTSGGGTGQAPSQFFLLFDLHTTRVLGFEASPGDKVIESYLREVTSTAVEPWAESLPLWDRYVYDNVATAVRGKLRGYVRGSGGGDGIGIGIDTAGRLPTAASGGGGAATAAAERASCAGKLMQLLPPLSGSCALSSSPYLDTFLFNYDERVVSPYIRTRPCLEQSIFFRLRNRLDRAVFRLDPGPYEAMMVRDRHLARNVTYLFHPDAPFVMAVFGVFQQNTVNINFRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXG6</accession>
    <name>A0ABQ5RXG6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045435224" description="Pherophorin domain-containing protein" evidence="1">
        <location>
            <begin position="32"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="21075" checksum="A7ED630E02E8CFED" modified="2025-10-08" version="1">MRMRNSSPLVYWCRYGVVWALLHLMAGTANTSMFPDRYARGTCSLRKELEENCQIKSKLGVNQTSGSFQGLLMEFGETDCVVSIEQCYPNTCYSFRDLTSQLPSGFLQLGSNGFVKSFQIISGGKSANFTKDGVSFFATSSKVLVRYSTVSCQEFYRYHGKIPLQKCNVPIEAITTIKAMRTPNALSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXM6</accession>
    <name>A0ABQ5RXM6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_004951</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056524">
        <property type="entry name" value="KIF6/9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF36">
        <property type="entry name" value="KINESIN HEAVY CHAIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23735">
        <property type="entry name" value="KIF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="5"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="449"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="747"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="485"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="755"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="91"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="788" mass="84525" checksum="69027F6942B0B97C" modified="2025-10-08" version="1">MVKQSVKVFVRTRPTATSGSGIKIGPDGQSVSVNVPKDESAGIINNQQETFSFKFDGVLENVSQEEAYTTVAHEVVDSLMSGYNGTIFCYGQTGAGKTFTMSGGGAGYAHRGIIPRALYHIFREIDLRVDKMYRVHVSYLEIYNEQLYDLLGDTPGTSDSLAVLEDSNNNAYVRGLTLIPVSSEEEALAQFFLGEQGRTNAGHVLNAESSRSHTVFTVHVEMRTSEAASERAILSKINLVDLAGSERTKKTNVTGQTLKEAQFINRSLSFLEQTVNALSRKDSYVPFRQTKLTAVLRDALGGNCKTIMIANIWGEPSHTEETLSTLRFASRVRTLTTDLAVNESNDPGLLLRRYERQLKELKAELAMRDTLGGRARVSYDDLTDDELRELHGIARRFLQGQAEPEELPADSLKRVREAYKALRAVHVALKSEMAQQLATLQRATEDGAAAAGAQNPMTSLPGVGDVDPRAAAGFTVGQAPLDARPPPRSELGDHHPPHAQTTALGPPPILKPQSSHYTGDAASTFGDASVPDSPSIARGSGAAAATRLAGIFAVPGDRNAIFRRYKSDVPEGRELTAALKAAAAALRETRGSIKTLGEAVNGAKRRVDELSAAVSQKRSAAPAGGDGEVLDSEAYALVQELKTVKAQYRADFDALKVAREELEPQIQAASLARAALLEAFDRWAATQSDATLKRMAATARNGPGPGDEEDELDAGEAFERMQLTRITEQDPDSLAYHAAMRRTALTAHGGGGGATSRPPAGAGGAKAAAAATRRMEATQALNRAGVRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXP2</accession>
    <name>A0ABQ5RXP2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004769</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="330"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="571"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="574"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="607"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="620"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1160" mass="129088" checksum="7AFE045FCE8A8F62" modified="2025-10-08" version="1">MIQNCLQRQADEAARRQAGQQQAQPPVQRQVPGQYRHLIASINSFLLGLQMAPGEWVVDTGAEAHVTTSSNDLVAFTPVEAACDTIFRRPNQRLVRPSGIGTVTFSSVRYNADVTVQQIHVVPDVHINLFSVKALQRELKLPVCAVLSTDSESYIEIGGEPAIEVYSRDGMFFIRDITKNGLVPMRNVATVEVARDEAQNNQGAPASGSGVIREPIVQGGTKWVRPFGSGVKSSVVSAFERGAWKKVRTEMTRAEIGVASVARKSCEVDPRLARAWVARATLLHRRMGHLGYGNLRKLVSSGMVTGAGTSELDVLAGKEEFCEPCALGMQTRQPHASTGTVTKNRLELVHMDLCGPLSVESAGGKSYFVTFYDDFTKFSSVSLVSQKSEVPKIVREALVLWERQTGDKVQRVHTDRGTEYVNNELSNVFREFGIFHEKTAPYSPEQNGAAERLNRILLQKMRAMLIDSGFPMQMWGEALLTANFLRNLSPTVNSDLTPWERFTGSKPDLSKLRVFGSRAYVMLPQRQRSKLDPVAVQGYLVGYEPGFSAWRVWVPLDGTIHISTDVKFDESARKLSTSSNTVDKTTDSDEEEEPDQSDSVAHHSFLDSPDPSNNNSNDDGGPEDENEELATDTDASPEPAPVPVRTSGRVNKGKQPERLKDFVVGATGQILSLKSFEVPIPETYEEAMSSPYAQWWREAMESEIKSLMVKGTYSLEELPLGMRALGVKWVFSVKTDKHGVVDRFKARLVVKGYLQREGIDFEDVFAPVNKHATLRILLAMAATGDMELHQMDVATAFLNGDLDEVIYVKQPPGFEGGPKIAARFHKALYGLRQASRSWYYTLREYLISIGFRVSQADPSLFMREENGALVYLLIYVDDVLIASRSIEALAKVKLQMMTRFEIRDLGEAEYFLGMEIRRDWEFRRICLTQNRLTNGLVEEFRMNDAKPKPVPMAPGTVLPQTKSDERLESEKYPYNVLVGKLLYIASCTRPDISQAVGVLTRHMACPGLVHWKAAKEVVRYLAGTVDFGLVFGGNRINEGLTGFSDADHAGCMDSRKSTTGYVFTLHGGAVTWSSKLQRSAAFSTMEAEYMAASEATKEALWMRQLLSDLGYELRPTKINCDNQSSIKVTKNPIISIRSKHISVRYHAVREGSCLWVSDYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXR7</accession>
    <name>A0ABQ5RXR7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004809</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF42">
        <property type="entry name" value="ZINC FINGER, CCHC-TYPE, RETROTRANSPOSON GAG DOMAIN, ASPARTIC PEPTIDASE DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="42164" checksum="CCDBF13908F0BFDE" modified="2025-10-08" version="1" fragment="single">MSEQLAIYRRCRRQYPGTAACVLLPAFCRRKAEASLRGMRHVHRFELGTVLFNGVDREGTLAPWPSTSYSVDVWYEAPDGGAVGYGPALSALTLSAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDNFISVAEVNALGLSSESSDCPQVTLADGGKHPIVGRITLSLSIGPLRVVTRPYVLQGLAETAPYIMGSLTLRQYRARIDMETATLHMCKGTLSIKVPFLSFEHVTPKEGETEAVPMVNFAVTAMATRIKPELVGRKEAVRLLQKGAHGLLLRPRVSSCVTTAQISADPEVEALLSQYEDVFKDIPGLPPMRPVDHTIPLVPGAQPTARPMYRLSPLELDEVKRQVTDLLAKGMIRPSTSPYSAPILFVGKKDGTLRMCIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY77</accession>
    <name>A0ABQ5RY77_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SOUL heme-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="entry name" value="Regulatory factor, effector binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018790">
        <property type="entry name" value="DUF2358"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_heme-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="HEME-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220:SF50">
        <property type="entry name" value="SOUL HEME-BINDING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10184">
        <property type="entry name" value="DUF2358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="Probable bacterial effector-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="46504" checksum="0B9E6838282082B9" modified="2025-10-08" version="1">MLGQILRPHSANLAHSCCEKSLIFPFHDHLRFVANVRRGLRPRFTKLGSTGSESPTAVKVTQNKSATPKFDMESKMRFLREDLTHLFDDQGIDASAYEAVVDFRDPITRYSSLSGYLFNIAFLRRAFDPRFVLHDMRPAEDEPYGITTRWTMSMRFTPAAALPTQKYWNPTITFTGTSTYVFNPASGKIFRHIDTWDSITNQDFFSVEGFLDFFKQLLSFYTTPNLETPEYVVLRRGRDYEVRQYEPYVVAETTMEAAGALNRDVLMAGAVSVNPAGPGNKAFNTLARYIFGDNRTAARMAMTTPVFSDTTGTMRFVIGPSAVKALPRLPAPNSPAVSLGREEGGVFAARVFGGYAGEEEASRQAEVLRASLERDGLKPASSVWTLARYNDPSTPAPFRRNEVLLPLKDFDMWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYE2</accession>
    <name>A0ABQ5RYE2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_005328</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUSP" evidence="10">
        <location>
            <begin position="3"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="443"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="65"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="232"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="333"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="926"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1363"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1470"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1605"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="412"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1387"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1476"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1622"/>
            <end position="1640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI62655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1640" mass="171543" checksum="9266C634AB07E87A" modified="2025-10-08" version="1">MTVAVHSEAAEVDALEKNASWTVGSIGYLVSTAWWNIWVQYSSYTGPGAASAASLLRLPGGSGPRPGPISNADLLPPGYSASKPQLPGATTPQGQTAPNPEEVPLRPGLVEQRDFVAVSADTWKHLYSTYGGGPAIARRIVTEAAPGTSGGTDAAERWRRLTLYPLQLEVLYKARDGSERSTVISIDPEASLSALKARACSVLCVSESEVALWDYTGGRPVRYLEAMPPSEAGTITTSTGGAVPRSVEDGTPAPPASSDGTANGAGRGGGMDVDSAAMTTRGPAAIESQTAAQGKVYAKDGVMGQQLREVPLMNGHLLGLRRRDAGVVSFDAGGEDACGSDNDNDTGQAARRKEAGKKTAVPVRMSIAEEAATTGVRRASAAGSSRSDGGSGTGVPIPPAPARTGGRPTGLTFGSNRQGNQRSSWGASEDNVIPRDGQPPGLAGLSNLGNTCFMNSSLQCLTHAVPLMRAFLGGAYLQDLNRTNPLGMKGELAEAFGSLMEQLWRGGLSAVTPRSFKAKIGRFAPQFSGYAQHDSQEFLAFLLDGLHEDTNRIRNKPYIEERDQPGRPDEEVAAEAWANYRARNDSLVVDHFQGLFKSTVDCPQCGFNSVKFDPFMYLSLPLPESRRRVVEVVLVRTDGSELPCRMALELSSGATLADLLRATAQVACLPAEVVSAPERHLLAARPLRGPSSTNDEVALLTDSRLRVADALDAGGSGSTYSLRSSSNSPDSGLVVYHYPDAARGPRAEGLAPVIVHFKRPDSRRTTSWSRGVWCGAPLVLYMPEDQPPYAPEQVIKVPSSPGNRSEWEISGDCPLAAALVEVLRPIQRLPLAMPPPPLPQGADSAVAHTDMQAATSAEGCSTKCLEEAEAVQAPMGQSAEDSVTVAEKGDGQEQLRLAEAVVPALVQMEEDDRLVIIAGADEELRPGTPTWDGARGGAGASDGSNETPIHRCSSNGKVIVTADLMEGMESLPAPAVETIAVSSTSGLAGGGYVGSSLQGTPEPSACGAMEDDVAMANPAPLPAVIILGPPESADAADATGLRPPGVEPFDMWVGQSTWGADDGGAAAGAAAAGQRDAGATAPTVGAPFTGPPFSLKLCNSKGESMLWGVIYDKDIKVEHHREFLLMFSDATSGADGAPYAAELLDGPSEHPSLGQHMRRVASGPQPVSLDACMTTFLQPERLAESDAWYCPRCKSHVCADKKLDLWSLPEVLVVHLKRFSYTRYSRNKLDTRVDFPLHGLDLGPYVMRPQAVSPMYDLFAVSNHYGSLGGGHYTAYAKLPSPFSGSMVDVHGAAAEAQSVERWYCFDDSHVSLVAPEAVRSPAAYVLFYRRRAEAAKDPPDVEDLLELLKQQRVELQHQMASTAGLPADGPTENTAGAGPGGSPDSGSDRQPEHDQDPDQGSRGAFLGSRRSGALGAVGEVSIAAAAAAAQAARGSEEAVPTPGDGEELVVEGRSMAAFSLGSRDPAVAGTYPHGRIGGSGGGGGFGVSSDSYGDAAAAEEPARITPMSVGNTYGDTAGVGTTGGRSTNLSSGERHYGEYGGNGAYDTNDFSVFALDDGGAGDSLADHCEGALIQPPGSPVEFELDRNNADVSELYQEVEDMEVLQDRLSGSDMASAGGNDQEVDPDDDVTGLGDDEGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYP2</accession>
    <name>A0ABQ5RYP2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004846</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11608">
        <property type="entry name" value="eIF2D_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11610">
        <property type="entry name" value="eIF2D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21156">
        <property type="entry name" value="PUA_eIF2d-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039757">
        <property type="entry name" value="EIF2D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048247">
        <property type="entry name" value="eIF2D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039759">
        <property type="entry name" value="eIF2D_SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048248">
        <property type="entry name" value="PUA_eIF2d-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057429">
        <property type="entry name" value="WH_eIF2D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12217">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12217:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26292">
        <property type="entry name" value="PUA_elF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25304">
        <property type="entry name" value="WHD_eIF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="3">
        <location>
            <begin position="587"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="214"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="671" mass="71013" checksum="7B9566D97C3BF3F7" modified="2025-10-08" version="1">MFKKPFSIGQSHKVSGADRKKLRRALEKTFGVSEEGLEALLPAKSGELECVKLASPSRVVLYLYDGQPILFDPNGKGDFVPTIFALWRLPTLLPQVYVKHPAVSRYIVGGADLMLPGVLLPPGGLPYFEKEQLVAICSPGNVAPVAVGTATMAATDAVVRILGSGATGPKGKLVELLQYYGDYLWSEVGARGVPNGGFLSDGVVPVGVTSVEATLTHEGGPDDGGDNDPWEGGADRAAESASDEAAARITRDLTLGSVSTTGDSDDTLTRRPEDLSSRPGTTNNDNNTNGNNGNSNRNPDGASTSSASLVGGEEARAAVDMDSLLQTALLQALHKSVKDADLPLNSSALWNQHLLPVRPAGSTLDVKKSKHKKMSKFLQFYGKLGLLTCKEDKHSGDVIVTSINRNSVLYTEYRAYKASATAAVEAAAATGGGNGATSAASGASGPAAAPSSSAAGAAGCIGPPGAPSVGELRIEELYKPGKELRPIFVDIGLNPEALYSASDAGEVVFEYVKAAGLEKGAPDNRTIVLNPALCDALFKGLIKKGETYPTHLAKADLREAFLRRMHLQCRISKGQQQVVRKGQLPVVQISTEKRQGNKRVTKVVGLEPFFVDLEQVAGECQRRFACSTNLVELPGKGAGHEVILQGSFVEQVADFLMQQYGIPKKYFQEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ35</accession>
    <name>A0ABQ5RZ35_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_005483</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045039">
        <property type="entry name" value="NSI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43626">
        <property type="entry name" value="ACYL-COA N-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43626:SF4">
        <property type="entry name" value="GCN5-RELATED N-ACETYLTRANSFERASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="101"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="254" mass="27734" checksum="217F8834DE862776" modified="2025-10-08" version="1">MAFSLIRSLPIKGHKSMSLGPPHWPCPMLVARGQSFRPFTVFVDFSFRRVRCHGFLGGLFGRGGTTTKQSPKPSLEDNLGPDVDELLLAETSQPDGSKAQILYRNGGPVAAADLEKLCTKVGWPARPLTKVEAALRNSFMVSSLVLRVTLPPLPGSPAEVAGADTAVSEQLIGLARATSDHAFNATIWDVLVDPEFQGQGLGKALVEQMVRSLLRQDISNITLFADSKVLDFYRQMGFEADPEGIKGMFWYPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0P2</accession>
    <name>A0ABQ5S0P2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005846</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058917">
        <property type="entry name" value="RESC6_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26188">
        <property type="entry name" value="RESC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAP" evidence="2">
        <location>
            <begin position="799"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="863" mass="92382" checksum="F41D77370837932E" modified="2025-10-08" version="1" fragment="single">VAAAEAAGAAGGAVQSVDDDGGGEYVPPVPRRRVPELVWLVPKAMDRIWERLALAAGTRAHQGKMAGDDLAKVAWAYARVGRADKRLYDKLSAAALVLLEPFMEKARTASVTGTARYGSLTIPGASGGAAMLARNSPPPMDATTVTNLVWAFVSIGRRDPQLLSALAAVASSYKGMYDNDQVATLAQSYSQAQYYHEPLCTAFGMVARRRHRHLTGSQLTALVRSLAELRHRDDELAGAVLGKLVWDKRPSLSAEQICDLMWACAHLGLDVQRGPTSPGAGVLGLDPVIATAKAVHRPPRSALSLQSSSSSPRQREPTIGGRGGPAGGGSTTVVLASALQGHCDGLPPASLARALWALSLLLPLPGHATLRMELVSELAEELANHPVDSYSREVLLQLYTAAHVLGSTMPQPAAAAAAAAAAAADAAAVTHDVRVGMHESETCDGDAIQPMETASMYDIVDEGSRFDEDAEIEHEGMTAEVAAVTGREQQPASRIVWRDRSRLFRKAATVAPAHRMYGTDIAASAASTSVTIGKHQERTQGQQLDQDMPHRQPPPPPLQYKRKDQDPLSLLSQLHQKNHEERRHGGGVTDIGPNPADGSWADVDWGCMRVAAPSVQAAAAVDGIHSVDRNHQHGVRNAERITRKAAAASAASVNALPASSYNVRRVYDEMSGRELWEVIPKGRGSGGIRDDSGDHADDPAARVLPTALLIACRAAAAEWAVSERRRLEELEAEEGELWRLDVAEALRYLTAAEPQPRWVTKDNTVLADLLLLPSPPSRPPWLSTSAASAAVPPPDPLAVLLVGDSDFTRNPPFVPLGSVRMRQQLLEAAGARVLIVPYFLWRWMEDTPDEQICMLANLLGSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0T5</accession>
    <name>A0ABQ5S0T5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Iron-sulfur cluster assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_006479</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008773">
        <property type="entry name" value="PRK11814.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="4">
        <location>
            <begin position="199"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="3">
        <location>
            <begin position="264"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="59391" checksum="082942FABF02BA4D" modified="2025-10-08" version="1">MASLLPSRTCTGASTSDSGRRSACILPSGLRAFQRSQPHLRVMTCRTATVEDTQISDEKRDIQKILDRPYKYGFKTIIESDTFPKGLNEDVVRAISAKKGEPEWMLDFRLKAYRKWLTMEEPKWSDNAYPEIDYQDVSYYSQPKIKEKLKSLDEVDPELLKTFEKLGIPLNEQKRLANVAVDAVFDSVSIATTFKEDLKKHGVIFCSISEALKEYPDLVKKYMGSVVPVGDNYFAALNSAVFSDGSFVFVPKGVKCPMELSTYFRINASETGQFERTLIVAEEGSYVSYLEGCTAPAYDSNQLHAAVVELYCEKDAEIKYSTVQNWYAGDVNGKGGIYNFVTKRGMCSGSNSKISWTQVETGSSITWKYPSVVLAGDNSVGEFYSVALTNNCQQADTGTKMIHVGRNTRSRIVSKGISAGTSRNCYRGLVQVMPSAKGARNFSQCDSMLIGDRAGANTYPYIQVREPSAVVEHEASTSKIGEDQLFYFQQRGIDAEKAVGAIISGFCREVFNELPLEFAAEVNELMSLKLEGTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1M3</accession>
    <name>A0ABQ5S1M3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_005940</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18019">
        <property type="entry name" value="DEXHc_Brr2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18021">
        <property type="entry name" value="DEXHc_Brr2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041094">
        <property type="entry name" value="Brr2_helicase_PWI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048863">
        <property type="entry name" value="BRR2_plug"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21188">
        <property type="entry name" value="BRR2_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18149">
        <property type="entry name" value="Helicase_PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="505"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="723"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="1352"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI63171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2167" mass="240685" checksum="FE8EFB2BCE845347" modified="2025-10-08" version="1">MSNKEKGGAEAYARFKQFDYKANSSLVLTSDTRTREYATEPSGEPETLWGRMKAKMGDRAQTNRPDAERKERLAKKKRDAAAAELELAVPSKGRGKRGGGAGASLSVLDLEQGGLYRPRTKETREAYEALLAIIHSLFGEQPQDVLRGAADEVLAVLKNKDLRDPERQKECLALLGSCDDDMFARLVALGKRVTDFVAEAEAGPGDTLDEELGVAVEFEEEEDDDDEEADEVLEVDDEDEGDDNQPEVDLRTTTMNDVDAGEEARDDGLKVQEVDAYWLQRRIARALGPGVEATRAQALAEQVLGVLGSPDDQRAVENELVLSLGFEHFELIKELIKNRLKIVWCTKLSRAETDEERDRIETEMGGSADTAAILAALRATRTSARDRQTAMERSIREEARRLRQGEAGARGDAGGGAIANRHQVALESLAFRQGSHLMSNKSVSLPQGSQRRAYKGYEEVEVPALKPKPFADGEKLRKVSELPEWARGAFTGMESLNRIQSRVADCAMFSGENMLVCAPTGAGKTNVAVLCIMHELGLHLRPDGTIDTSAFKIVYVAPMKALVAEMVGNFTKRLTEKYGIKVRELTGDINLSKAEIEDTQIIVTTPEKWDIITRKSDDRTYANMVRLLIVDEIHLLHDDRGPVLEAIISRTIRQVESTQEMIRIVGLSATLPNYEDVAVFLRVKPDKGLFYFDNSYRPCPLAQQYIGVSVKKPLQRFQLMNEICYNKVLECAGRHQVLVFVHSRKETAKTARFIKETALAADALARFMRSDSATREILQTEAENCKDSDLRDLLPFGFAIHHAGMTRADRTLVEDLFADGHVQVLVSTATLAWGVNLPAHTVIIKGTQIYNPVKGAWDELSPQDVMQMMGRAGRPQYDSFGEGIIITGHSELQFYLSLFNQQLPIESQFIKALADNLNAEVVLGTVSNLKDAAHWLGYSYLYVRMLRSPALYGVPPADLDTDPLLQERRLDLAHSAGLMLDKHSLVRYDRKTGNFQTTDLGRIASHYYVSYTTIAHFNEHLKPTMTDIELLRLFSLAEEFRYMVVRDEEKLELAKLVERVPIPVKESLDEPTAKVNVLLQAYISNLKLEGLALTSDMVYVTQSAGRLMRCLFEICLRRGWAGLTDRALALTKMVNYRMWGSQSPLRQFKGVPNDVLVKLEKRDLPWERFYDLTSQELGELIRVPKMGKSLHKLIHQFPRLELAAHVQPITRTCLKIDLTITPDFAWEDKVHGFVEPFWIFVEDQDSEQILHHQYWLLKKSNAGEEHVVAFTVPISEPVPPQYFVRVVSDRWLSCETTLPVSFRHLILPTKFPPPTELLDLQPLPVSALRNAAFESLYKGLSHFNPIQTQVFTALYNSDDNALIAAPTGSGKTICAEFAILRMLSRAAEGKCTARCVYIAPHESMARDVAVAWTAKFSDGLGVEVTQLTGETAADLKLLERGNIVVATPQQWDVMSRRWKQRKNVKDVALFIVDELHLIGGPKGPTLEVITSRMRYISSQADKPIRIVGLCHSAANAKDLGDWIGATVHGLFNFPPGSRPVPLEVHVLGFDITNFEARMQAMSRPTYSAISTHAAGQKPALVFVPTRKHARLAALDLLTYAAADGEPLKFCAASESDLAPYLPRVRDAALRHSLQYGVGFCHETMPAGDQQVVRLLFDSGAIQVLVVTAAMCWGLGNTVAHLVVVAGTQYYDGSGLGASDYPITDLIQMIGRASRPQVDDCGKVVLMCASHRKEYYKRFLLEPLPVESHLDHFLHDHFVAEIVTKTIENKQDAVDYLTWTFYYRRLAQNPNYYNMAGVSHRHLSDHLSELVENTLSDLETSKVIAIEDDMELSPLNLGMIAAYYHIAYTTIELFAASLAAKTKIKGLLEILANASEFDGLEVRPGEETAIQKLLIHSPVSVTQPRPSDPHCKANVLLQAHLSRTPLGGDLALDQKEVVRDSVRLLQAIVDVIASNGWLSPALAAMEMSQMATQALWDKDSPLLQLPHVTPDVAARLEAAGCSSVFELLEMADEPRREALGAAVSEAQLAEIATVANRYPDINVSYEVVGGDEEVLAGEAVTVVVSLEREMDDEAGGEVGPVPAPHFPGRRDEGWWLVVGDPKANSLLAIKRVNLAKATRTKLEFSAPSGGSDGAAHLTLFFMCDSWMGCDQEYEINLKIAPNDDAMEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S206</accession>
    <name>A0ABQ5S206_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="9">1.1.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_006998</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="9"/>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="11">
        <location>
            <begin position="201"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI63880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="669" mass="70372" checksum="504023494A0044B4" modified="2025-10-08" version="1">MQPELLLYSTLQLFVKRCLNLRCPSGDAATVAAGLRCLQLRGRCVSYPLHRAPAAARDGPISSNKLRDHFKNTGRWDESPDRRPQGRAYYVPLAAADPSTFGRPDRLSLRDHPTPSGTATTNTAATSMAGPREPFLVRRLFGTLPAAHKSGAWASIPQDGAPPRRQPSAELRARLAALLSGRVSEARDTLVQHGTDESYHESLPPDLVVFPESTEEVAAVVAACAAERTPVVPYGAGTSIEGHVGALYGGVCLDLGRMDKVLSVTPEDMDCRVQAGVTRQQLNDHLRDSGLFFPVDPGADATLGGMAATRASGTNAVRYGTMRDAVMGLTAVLADGRVVRTGRRCRKSSAGYDLTGLLVGSEGSLAVITEVALRLHPVPEAVAAAVVTFSPQAGAERDGKGVDSGGGGGGGGGGLRGAVECVVAVMGCGVPLARVELLDELSLQAVNKYSGTNFTLAPTLFFEFHGSSAAVSEQAKLVGSLAEEYGGSRFEWATSPEERARLWKARHTAYWAAISLRPGCKGFPTDVCVPISRLTECVMESQEDARQAGLVAPIVGHVGDGNFHMMLVVDPREGGEVARARGVVGRMVHRALGMQGTCTGEHGIGYGKLPYLLAEHGRASLEVMHAVKTALDPHDIMNPGKLGSAPDVLERLAGLAAADAMESAAASAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S228</accession>
    <name>A0ABQ5S228_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006481</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF37">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE 5-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="433"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="115"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="474" mass="53444" checksum="127ABFB1FE02EF2F" modified="2025-10-08" version="1">MARLFSKLKAIDFFKKIPSDLTEATLTGAWLSLLAAVIMVFLFAAELASFLSTTTSSQLVVDRSPPNELLKLNFNISFPALSCEFATVDVSDSLGTRRMNLTKTVRKMAIDVEMVRVGSAVEDTSHKPGPKYDEEGRFDEDQDIDITAPLSHVNFEATLARYPIVIINFYAPWCHWCQRLEPTWEAATKEVHDKYPEWDGRIRFAKVDCTQEMELCRDHFIQGFPSIRVFRKGHDDIMIGETHQHESYLGDRTKESLVAFADSLVPSAGQPHRKHAALAAAPQTSGCNLAGFVMVKKVPGTLSFVARSEGHSFDHAWMNMTHLVHSFHVGTRPSPRKYQQLRRLHPAGLTADWADKLRDQFFLSEHAQSTHEHYLQVVLTTIEPRRSRHAGSYDAYEYTAHSHTYQSDAIPSARITYDLSPIQILVQETARPWYHFLTTSCAIIGGVFTVAGILDALLYQSMKVVKKMNLGKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S262</accession>
    <name>A0ABQ5S262_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">threonine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_006748</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF44">
        <property type="entry name" value="THREONINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="349"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI63699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="722" mass="80257" checksum="831AA1D0BE441EA7" modified="2025-10-08" version="1" fragment="single">RNKMRPILQKRANAACASCKRGALGWLRASQFAPTPTTSGPCIVLNGNLPPLGITARLQSSWSRSSPLLAATVEAPAASEAPLEQTSDTIKQSVFMELKTSDESEQLLRIRHSCAHIMAMAVQRLYKGVQVTIGPWTERGFFYDFQWPADSPINEKDLKKIKKEMAKIIKADLPFVREEVTPAEAEARIRAAGEPYKLEILQSILQRNASASITIYHIGEPAAPAAPAAPAFVDAPGAAAAASAAPSKALAPSGVKPWWDLCAGPHVARTGDINPDAVELESIAGAYWRGDEKNAMLTRIYGTAWETPEQLKAYEQLKEEAAKRDHRKLGAELSLFSIQENSGGGLVYWHPNGAMVRHLIETFWKELHLSRGYQLVYSPHIAKADLWKTSGHLDFYRENMYEQMKVEDETYQLRPMNCPFHISVYKDGYYSYRDLPLRLAELGTVYRYERSGTMHGLFRVRGFTQDDAHIFCLPNQITSEIKGVLDLVEETLGTFGFHDYEVNLSTRPEKSVGEDDIWAMAEAALVEALGVKGWRYQVDAGGGAFYGPKIDIKIRDAIGRKWQCSTIQLDFNLPERFDMHYMDSSASKQRPIMIHRAIFGSLERFFGILIENYMGAFPLWLAPVQVRLLTVNDMVAEYARDIAAQMKRAGIRAEVLGEASIGKLIRNAEKAKTPVICVVGAKEAENNTLSVRLYGGKELGALPTQEVVGRIVMAISTKSGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S389</accession>
    <name>A0ABQ5S389_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">serine O-acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006887</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.30" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03354">
        <property type="entry name" value="LbH_SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3130.10">
        <property type="entry name" value="serine acetyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045304">
        <property type="entry name" value="LbH_SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010493">
        <property type="entry name" value="Ser_AcTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042122">
        <property type="entry name" value="Ser_AcTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005881">
        <property type="entry name" value="Ser_O-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053376">
        <property type="entry name" value="Serine_acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01172">
        <property type="entry name" value="cysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041874">
        <property type="entry name" value="EPS_EpsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42811">
        <property type="entry name" value="SERINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06426">
        <property type="entry name" value="SATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00971">
        <property type="entry name" value="SATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine acetyltransferase N-terminal" evidence="7">
        <location>
            <begin position="129"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="388" mass="42556" checksum="074BF5B5D7D0269E" modified="2025-10-08" version="1">MMTQRAFFYDVAAPSTSTPLSLVSRKTSSQICNGRNAAAARREQLQHRLISTVITRTMARDRLSEQAREDLDAVRVRLNHQLKLIITPSVTGEAAPTLQKANDCQCDRHTYHPSQGWRRQPHHLSKDQLWDRILEEAHQDATAEPALASNLYSTVLTHHSFESTMAFSLSNKLASPTLVGMQLMRLIQEVYEDDAEVVESCLADLQAVYDRDPACDRYTQAILYFKGFQAVQSYRIAHWLWQKGRKSLALAIQSRMSEVFHVDVHPAAVLGRGIMIDHATGVVIGETAVVGDNVSMLHHVTLGGSGTGRGVRHPTIGHGVLLGAGVTVLGPVTVGAGSKLGAGSVVVSDIPCHSVAVGVPARIIKRDLLKEAVKDMDQCVEFVLDYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S411</accession>
    <name>A0ABQ5S411_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007958</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09275">
        <property type="entry name" value="RNase_HI_RT_DIRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="29179" checksum="B5298AC9C54D28CC" modified="2025-10-08" version="1">MQPVGWSGRIRLSRGSLRDLDWWADIPTRHCSAAIHIGPAAVDLSVDASRHSWGATLHGRIARGYWSSNDYLAHINWKELRAVRHALESFLPYVTQRVVLIREDNTCTQAVLGRLSSRSPCLHEEMRCLWSFLQEHSIILQVERVASAENEADAASRWIDRDDYRLHPHLFQLIENRFGPHDVDLFASHINAQLPCLFSRHHCPGSSGVHTLDWAQRLRLPTPGPGPKPFLMAVVQKVREEQACVTLVVPYWTPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S447</accession>
    <name>A0ABQ5S447_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plastid-encoded RNA polymerase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007578</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="5">
        <location>
            <begin position="23"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="343" mass="38010" checksum="FB83ACF741545890" modified="2025-10-08" version="1">MYSRPSRNFRKPTIIIRRHEPDYMVFELKNTDVSSANALRRVMIAEVPTIAIDLVEMENNTTVLNDEFIAHRLGLLPLVSTEATAWKRPFEWSNDADMIETAFSLDVTCNVDGVMDVTSNDLVPLNPEHRVFPVNYQNPDEKPIVICKIRRGQQLKLVARARKGIGKDHAKFIPVATAVFQYKPHIVLSHSAMAEMTEDEKKIFVNSDPSKTFKFNPVTRMIEVENEEAYMYDEECIIKARELGRPDLVTITQLQDHFIFRVEGTGVLQVADVVRQGIEILHKKCLDLEAGVQEALAVSKGQPLPAPPGEAAMDMGGVGPGGGGMDMGMGGMDMGGPGGGSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4Q5</accession>
    <name>A0ABQ5S4Q5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="723"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1245"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1332"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1386"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1442"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1456"/>
            <end position="1475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1475" mass="158067" checksum="4E9DC9C5361067D5" modified="2025-10-08" version="1">MTAQSFRHRATTFLRTELAKNNKGSDEIPCCVVAYWYNNRINAVTGRKPLPANGGMQNADAVGRPKTRSPGVRHTSGSCKLPKLHVSYALGALATYLSTQIAPKPLIGAPGLPWLLASAYSLRCLHGAADGNAPMASTGLPSASSVTVNENAIAQQDITDIKGEPNLTTHLKKRKNLEIFEQIQDCTDAEDLLDLLASAVGVSKPAVSVAVHSGPPSVPDRAVVEILLRLSTLPRSQPPPVSEEVQARLRQRRQRREDGDAVPRPPPPAADPATPGSDEWWQRRRALVVGLFRQLVPLYGTMDLTTRVALLESCSHLTWLLGYCQELKPARHGDDNDVSAAAGDDRRHPAQALLDAVLNQGRGLKLVMAASAQLSPSCDTDHVGNPAQPSASLAARVLASMGRLRYQSGEALANLLPPLMARRRNLKAADLVCVVTAVAELRPIADQYVSWSDVCDGLAAPGALRQLTAAELVRLAQALADVGAIWSDGGKGRDRDPDSDVESSSPSYTEEDLDEIVEYNISRRRRHGHGHGWGGARPGDREHDMMYGAVAERTGEWTGADAEERLLTSLGREVSERAQLGGDLSGGQVVQLMVATAKMGLRDTNVLKPLAAAARRHLPSLRPDEVSQLMCATARMGFVDPALIDAVSYTVSAVQSTNPVSYNVRSLAIVCWALACHGRPCPDLFSESTAIALINGMRIRPHMSSTPSPPNLPPSPHLMGVLLQPPHPLPEPRISARPAEGSDGMHEEEASAALPHPKQQQQLQLRPYEVANSLWAFARQNTRHRGLMRAAVQRCADWQRRGRLPRSNLIKVIWAATKMGYVPRANGGADSGSGWRRSREEVVTGLGGGWEHDDDVHSDALLLAERVAELVIGQGQRFTGPDLVHAAEALEPYCTAGGPMHDKYGRQVYQRLMEVAQDRVASLRPDACVALLSTLSRATVASEGSDVGPTSAPASPPPLSSLNSRLLLRAQQRLMGPLESGKLSIEQLLQVMEVLRRADARNSPSLPGMEQQLLRLVAQADAGDGEGAVVAAMARPLRGDQAMAALMTLGSLQRHGAAAELFRLTHRQVTLSLEQQSPEGITRLLRVLGAAVLRDRRQTAAAVAAMTPAPTATVRKVRNRFSRSAGRQQQPESVREEPQAALAAAVLGSGLAAFLSASWRSLGRPGELERLSTSQLVDAVWGLAACGYDDPRGYDELVCRLRGRVKNLPAWSLARIAAAMHGHCLNNHPTAVATSATVNAISGARSSLGGTVPQTGGSGGVRPPSHLERHPELAERIAKYGTRELTRLGSGAKPQDLATLVEVFASAAPQYSGLFLAAAHRARRWLKEAAELEEETEARSGGATDRDEGGGGGGGQTGDLREAATRVRAACEAAGYDVSEVLGRVSEPARSGGSSDGAERGNRRGVSASSRFGKVYGGAPVPVLPEGRESLRGLLSPWGYRGESGAHWAPGSGNSGRERAWRRREDDRRIQDLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6N2</accession>
    <name>A0ABQ5S6N2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_009155</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="549"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="34"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="882"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="916"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="945"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="890"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="926"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="992"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1006"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="576"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI65582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1036" mass="111445" checksum="1B8DD92F81873DB8" modified="2025-10-08" version="1">MTAAEAQTPCTGLASCQIGIVTEDATVISVTNFETGPDEAANGKLITKTSDEDSAEAAPALLKSEKTPENPPFESKCSSESPEDEAKPGAELGAASGTESVASPVKGGRRCAIRIPGYFWVALAVLVIFEALAVTGAILYARSVHNDARRATADVARSSARAIETVLDHLIAPVMSLAHLVHLQPHWPTVNQSFPVFSEAIYKTNPYYQIELCPYGIVGSMAMPWMNATGNITAEEWPAILKTFDNGLDMLNPERPSFLHEIAAIHHDGSIAMLGPWYADGELSSGAFVVRYSIFLPPGQWDMPPSSIQHCPTDVCNTPDGLTWWGWSNAVFAWKTVIPALKGLEERNMRFTLLPDPRANNQSALAMSEALPNPDECEIVSVNVYGWMHWTFCVEPPGGFGPRWRVGLIVGVTALSILLAVLVGLVLRSRHQAVKYLSRQLATNRQLAIAKEEAEEGRRTLEAEKRVMDALLQRQRNLIELFGKEDELLNAVADGDEAAVQRRLKAVNGGSQNSNLSRESTAVDRIEAVRRQLTGVRSMKQSASGADDIKLMEMLGEGSFGKVYRGLWRGTDVAVKTMVLPANMSGAEKREKMAIMEAAISSSLAHPNIVQTYTYFIKTVREEALSDSDTSLATDGILVGGTDDNSTIGPGASSIGKTFDNPSARNNTPAVHGYEVRLVLEFCDRGCLRDALDGNAFLTHAGVNYGGVLDTAADIAKAMLHLHIANVLHGDLKARNVMLKSSGTEGRGVICKVADFGLAVKMDTMEQTHMSGMYQGTLTHMAPELLLHGRMSKAADVYAFGVTLWELFTGGHAFQGVPRALLGHQIAQERRRPVFPPFVPEPYVRLAEECWAHRPENRPSFEVIMERLAMMRSTVPGSAAPLSAYNIGEPPDPPPQPPLPKVDTLQARLNVVCNPTNESGSGLGAGLSSKSSSGGSSINWAFAKSSHRTPLAVVEEDEREINMSSKKLPSVGETGGSPCSHSPNHHHPRSSLENIQDGTSGHSDEVGAADGAAGSDRGSPSRPATGPPARSAAALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6U7</accession>
    <name>A0ABQ5S6U7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lon N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46732">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE LA (LON) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46732:SF5">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE LA (LON) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="2">
        <location>
            <begin position="47"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="345" mass="37284" checksum="06D2F243BE7E3866" modified="2025-10-08" version="1">MSSSFGLRTAKPCSPPNVPRREGHPARVARRVFAGATMIDTSIFAVSLFPSNKVLLPTATGTLHVFEQRFLQMFDDLAEKAGGNILDANGIRFGHILSPSVAPPALLENAISGMPRLAVMATVKRVKRNDDGSLLLQYEGSKRIKLINIWQSQPYMIAAATFMTDNVSPTEEVLVDSLEWDLYRQLEEVGRLSKQLRTSEDAPAGLPDSIPRYAPPPPPSRPRTIAEYLTDAGHPAGTSISMWQRHGSVYGNVKQGKDATQDPYAVLSERLGKDTRQELFSFAAACMLELGAAESLALLTSTDRAARLQWTSAAVTPYLQEQRAKASLVKALGHSGGSGGGTPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7C5</accession>
    <name>A0ABQ5S7C5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="2">
        <location>
            <begin position="189"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="569"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="755"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="773"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="796" mass="86055" checksum="03C045D95DB46F78" modified="2025-10-08" version="1" fragment="single">MTTRCNLRWKSGAADMAMAPPLRAVPQLLWAVAAGVAFLWAAAAQPLETDLPGEVAGVLLMQSMSFFKDGDLWGASLLADDDIMYQIEFCSSVGVEGLMPNARVRLSYQKISGGVIYTCEVPKLVEGQGGEGDRRRLFGGVISTPKSPSFLVFLTTFCGYDKPAAVTDEMVYDLFYGTGLLNKTLTVDDYFNGTGPVQRNMSGYYTSCSYGQVRAPTVRVMGPVEVPCNGSVDHLLFTYPGGNNFSTSTCRGFNVEKWQYYLQNWAKSEGVESNQFTHQVMIVPMGFTLATPDCNGFIGVSTYGARLLGTVGNPFGTGFIWLSGNAFSRLEFWLHEAGHNLGLAHADTVKGCSTDDQCDWTCTMGAVSGQGIRCFNAPHNWQLGWGSAVRQLTEADLRIGKYLPLVVPPQSSGPASAVMLVMLSGATANQRFYVSARMNTPPFDLPFLASQNQKVFLLVHTYSGTETMAISRTELQAQLAVGETYSHNITGLTVWFSRWLEGQGALTSFCRRIGATEQVCGDGIDDDCDFLADVDDPDCIGRMPGGGSSEEGEFVVSVEPTVVQPLLRAPPRIALRPSPKLPPRPQWSPTVGPSPPPPPPRYDPPLPRPPPPIKPSPPPPPSPRPYPPSPRPPRPLPPSPPPPRPSPPSPPSPRPPPSPPPPRPPPFRPPPPWRPPPRPQPSPSPPSLPLRPPQPRRLPPGRPPPLRPQPSPTPPSKRNPSLPRPGPPSLRPSPPPRPPAPPPLPLPLRNPSPMKPSSSSLKRPPTTRVQRLPPSPPSPQPPPFPPSPLPPLPPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7N8</accession>
    <name>A0ABQ5S7N8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008708</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047051794" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="44"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="380"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="539" mass="57434" checksum="D66838F35E3CE483" modified="2025-10-08" version="1">MTRMPYLALLVATALAVGWVASAQDFGDDEFSGNRDQTGTIPNFPYRSCNTSNGAYRLAPVWVPLGISQYCFKIEVRQDAKSCSGACCTASLHKIEFNVSSSCLVAGSSVTATLNGMPTRVGAAFDKPLYGLPGSAVLRITQLGLDPMTAQGAQLCITLKPNRGRQGCTTLEQLCSSPGFPAGTCTAATFDVACDCCPVTQVIQARPPPPPPPPPPPPPPPPPSPPPPPPPPPSPSITPGYRKCELCISAKLEPPAIDLQPYRYSAADCAFIQKGISDIINPVVNASSLPILSLFAPNSTLCYPDRVAVCGIFNGTDDDLLKKLNETVQNTYLDVFLSIATLGDICRPKLQGYTVEITTDGNSCLDVSKSINCFLPPTPFPNCTCNTTQGVLPFLVSPSYYSRASPFFGSSVTEYCFNISTLPTANIVPSTCYKTGDLLSKLEWYANITLRSVVKGYVITPAGGPSKKSSPSWGSTDASSLKASINWTVAQANGGVVCVALQKPYKMSDLCFNGQCYISLFNRESKDNCCPTFRSSLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7T2</accession>
    <name>A0ABQ5S7T2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="470" mass="51679" checksum="74F36480935BF3BD" modified="2025-10-08" version="1">MRLKVQMSPLGSGLTVEEAIDLYCGEGEQILQWIGYAACSRLAYKRGEVYGRYVPQSVNNKDGQPLDVDIVVNEMFNDGDELFVEFSNGPVPYKVRWEGRPRTPPFRWGDGVEVLPPHDTWLRELDLKAEGLGALIEPDLMRADAAVAEKDLEKVKALLVQYAGAVQMMFLLQSADGSTNNTTSGEQLGNLTLPQFRSAMAAAKVITPRFPPERVDEIFTSVATAETTLARKVEAKSGVSTFDLLDFMLALGHLAAHRYAAENPSQAAYNHLSVKVTALFRECFAASTFPELQRRLERFSPALNNSNAMLLLRKGRKLTEQTLDTCQLKRTRGAPVRVDLRWLCNHLSKWGMLGREFNLQELALIAVFAKQRSTDPETFVLHPQPLEYDYDQFERLLLGAAWTTFNNRKKAAPGGGGGGGGGGGADAGGGFEEFLGETLDNVYKRAGVLVPVAVHDDGEDEDGHGPMGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7X7</accession>
    <name>A0ABQ5S7X7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009713</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046815024" evidence="3">
        <location>
            <begin position="40"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="620" mass="66049" checksum="4B20234F4577F757" modified="2025-10-08" version="1">MRHRLRFLLRSQSYSGLSCSYGLLTVLLAMVVQIHLCQGATMHPHPNCDGPCDETNRMCRSTTCDKAWDSCMPGTVQNTSWMEANTNSNDCYWTECTPGGCPEGFEQIGKELCFDVKQCPQHVGVCNKEHCCPLEPRVECCKLLPCDVTYEGCQMPQHSFNTSTSVFLPHGSTTMSSFYTAAAVFMGGGTCDDVRVWGSCQCDDGRIRPGCELSNSSTWYLQTPGNPSESSTANPNGTLLPPDLSITCVAVPAEESATQGGSQQRHREGMPRGALVALIIIGCLLLAGCTAGVVLWCCGLLPDMHNLRIRSLTSYEPVFTGQSYTPSMGGSSSSRRRAPPSSSFYEDIPWTRFCAAVTEVSRRVLYGKRYIEESASAAAAAERYRTLNSLGRRRRGGGGGGVVAAPGDEEEPESTWPPGKVRPTLGQVGLTVYPSHRGKVLNDDDLGVDEVFTLDDLGANSGDAIMTAAWRPMGHQLPVSRSATAAAAGTLPPFFRAISGTSVATVQSPAGPFRGPSISSTTGTATATAAAAGLNVRSAGGRTCSSTTATPSEHSRQRLNQFGLSKDLGNDDDGKVYGGGHDVIHTRPLVLGDSGVEEDAVPPDVDAPLRWLPSPQVTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8U9</accession>
    <name>A0ABQ5S8U9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="143" mass="13308" checksum="0B0AC3709042DD01" modified="2025-10-08" version="1" fragment="single">GGGGSSDQRGSGQPGAPPSPLRGRPGGSASQSAVGVGAAGVGVGAVGSVAGRYGSRVADSPLRSAVATDPNVRGVAGQADVLKGPAAAAAANGASAAADRLAGDFSRLQGPSAVAAAAAGMPRQAGANAALPYVLANGRVKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S946</accession>
    <name>A0ABQ5S946_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Plastid division protein CDP1-like 2nd alpha solenoid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057137">
        <property type="entry name" value="CDP1-like_a_solenoid_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044685">
        <property type="entry name" value="CPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33925">
        <property type="entry name" value="PLASTID DIVISION PROTEIN CDP1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33925:SF1">
        <property type="entry name" value="PROTEIN ACCUMULATION AND REPLICATION OF CHLOROPLASTS 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23468">
        <property type="entry name" value="ARC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastid division protein CDP1-like 2nd alpha solenoid" evidence="2">
        <location>
            <begin position="506"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="743"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="743"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="768"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1461" mass="146909" checksum="62260DB9BF1C0B00" modified="2025-10-08" version="1">MLSQRNLSAAAEPYLGTAHWRYRAIPTRPLVSCAAHRTAGAVTNGLDVFSRTGDAANANVAYSVKLDAEHAFATDENRCIVNNGPPEAPTQHLQLESSVLLHQQEVMGQCYSPTTVSAMARLLGHHDGQELSIDAGQASVVVLPKAHLLSILKAGSGTPLDEASAALALLCEAGQYDIVVQYGSPLLKAASAAMPFSPLGVSRQQPVPHLEQLTADLALSIALAYISLAGGGGGPAGAASSASVSSGGSSGPIPAATEANTGGAALTLMDMEEVFSHLESALQVLERHCVAPPLQKEVAYCLQGVAVAFAEDVLRSTPPVLQLSPAAAPPSAPGATPALTQRKGGDGEVVDGNVGWGAVADSSGRPAGVGKETVTSSLSSSVATAAAARRTKAVCMLRSVLWRCAPQNQSNQHSQDRHQQHRGDTAPGVTQGSVTAAGSAPVVGAVIAELRNGPPAASSGDSRGWVPELTPDERSELIAPLRGLLAASEHIALYGPVASGQKPYGAVQLSDQELYDLAIAHLAQGVSTGWPQHVLQALSYFERVAQQQQQYGLSGAAGLRALAAATAAAADVSFERSVCEALLGRRTTAAGPVDLPPPASSTAMSAPPSGTAAAAAGSVATESPHYNAFGAESANDGDELALLRRLEAQLILAAGGGGGGASGAAAGLNGASSDAVSFEVKERVGDGVSDEEGAAQEGLAAPRDVAAAAAAKTAKNAFGVTPAATMAGSRTVPNASAAGATATATTMAATGPPSSSSGIRGSTASPGGQCQHQQHTASENLGVRPRVKADRVDDVARKYGWAESWLELSVMPCFPETAGAGSVSLARWFSDPRVILFNKLLAVRSGAEVTAALGSAVAHVSDASRQRLWQLWQAAAQAVGSATGQSAHHHQLQHQPAVVATASLKAPAASEAVVVAAVAGGGGEHKGAGGGGIGGAPLAGVLWAQSHAALQDVGPRAAAAASAMAAAAAAVGSAAVSSPRAVAAAAKSIVPFGLARWGSSEGPRSPNSNGGGRIAVTAQALPQTVATAATTASVVGRSIVTEASGGSGSGVAIPTSTPTTVIPAVLPVAGELVDVGMAPPPAGGGGSGAAAVGTADAANITGTADTASSSALWAPDAEGHLHPRPRMVTRVVMQRAAEAAASIKDRVAILPVAPRDWRIAGWDPAAVDEAMSREAVRRVAAVAAQAICLGALAAYVGNRILARGALQATAASGYGAAAAAVAPTVDASTAQVAAAARAVARWLDSAGMGGAAQDALGRWPTVVKLGGWPPAEALPLHVQPASAESLARLAEQYGRGTQLDETAAAALLRGWSAARAAAPSLSDPRDRSELLSGLLGGRALRVARAEAERNARMGKRVQVQRIDVEIEQLRFRPASAQAATSFFYGHSQAGAAETTVTAVVVTAGTETNRASSVPLSYCSRERLTLTFRRLPLEGGPAATPAAPASVWRLVDVRDAPPAVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9F2</accession>
    <name>A0ABQ5S9F2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_009858</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="5">
        <location>
            <begin position="378"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1346"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1456"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1776"/>
            <end position="1807"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1939"/>
            <end position="1980"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2061"/>
            <end position="2095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="536"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1161"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1318"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1514"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1835"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2199"/>
            <end position="2223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="536"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1182"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1330"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1519"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1838"/>
            <end position="1849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="2209"/>
            <end position="2223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2244" mass="232516" checksum="347B818F3360F8C7" modified="2025-10-08" version="1">MGKGGGCGASKAARVPSMSAQPVAQEEEDESFWHMYVSWTNEDEQSPERPVTMPPPNAAASSLTTGAVRLSYVLDFVTTRLPMGDPNMPTWSVALNLVQPATLDLGVPYTSLPEMAQHVLPYGTTKPYYFVSHAWSRPFREAVDMLAHHFRGCDPGGVYVWFDIFAIRQQSFTAAPTGPDMVLLEAALRNSAGVLLCLDEAGLALRRTWCLYEAWVGATAPAAGAPAAAAAAGQGAGATAEAKEAEETEPGLAAATDGAAGDAGGAAGNGNITAISAAVPPPQPLPQPPIGVTALTPRAMLPEERARLFSRIDIRQSRCKYKVDRDALLASARTAAGPGGLDAVNSMIRLALMLRPHVPEVYPELNLGRMIEVPELWDMSRVDQWLSLPQTSNFFPAMAIYGPACSGKTVATAAIARHLRMMGIPVAVHFCNVSDVRTLEPVAVVISLALQLAQAPGIGPRLLRQYGELPAGLFADLVSYNNTPLAVEAAVEALLVQPLQRLLQPDTDADGDPGANVVAAGAAKDDAAVTPSTVLPGGGGGGGGGRGWNENGDVGTLGGGCDEDLEGAGGGGRGLHAVGAKSLRAPTNWNGPPEVVVLIIDGVDQADGAIMGGSAPFDNQVLQLIQEHLPKLPSFVRCILTSRSLSYIIPRLAERFSPLEMAPDMLRPIGPFMRNVRLAIASIDPALGRRHRAAAAANLVTKSRGSMLYIKMALCCLELERDVAVAEAEADVMLAAVASLNGPGNSAAAAAAAAAAANPVQQDGVRRTLSRLASTKKQTSNHQLPSSLGGLLERYLTTRLRLLNSTELRNQAATVLAVLAAAREPLTPMQLAATVGVNSSGWYGTKTYAGAGAAARGMALLAAPPVATERMLQDFVVRGLGALVRLRGSPGSWAQEVLLVHSAVSEWLQRTPKPRVLLGGSVIVDLAAGNRALACYCRDDALSCLYRDHSQPYGYSLRHIFLHVGLAAMAASAAEAAAAAGEGSGGGGGGPMSPSSVATTTRSSGIAQLRSRSGTYSYNSTTAAVALAAALAAAAASADWIATLELLLQRLDLWTSIYTAGAGGAARWVVLALASLPMCSTPSADPGSTDSQLLMILAEARRWLLLYHSQLCWFPLAVVASAATLPEDSALRSRALEFSAGPDPWEYHQPPATTITVMPAHKRAATRSAAAQAPPPPPSAPPASNDGAATTTAAAAAAAAAAATLTDPLEAEHMLLLSTGDVWSCCTQTLRTGLGRVLHVAMQPAGRMVATVHDCERGVWLWDIWGAKPILAFAGCCEMGSNPTAAFFTPDGRKLVCAARSVFVWEVATGRRLLELHRVSPQRKGSGGTPLPPPGSVPPADPDGPITALALSPGGSRAAAGHANGTIRVWDLASGALLAATQVPHCRAVTALAFNIRGGETLVTAHGEGGDAAPKAQAPVAPEELGDVPTAFIKADELPIHVWRVHRSSLKELEVLKGHTGRVNTIVFSTHNRTMITGSADETIRTWWDDPKTPQSDYRQIFILGGVGQGVVHTIQFSPAPPPPPPPPSQQHTSRKSSSMAASLLMAAKASGSTNNADAAGNADGETPVVDFTAPSAPCLATAAFGTNADLPRVASGALTPDAASAGGGFPEVARGNVAAVAAAAVGGAGRGDDEDPSAEDILGDVPVMAYSCGNGIIRLWSPTRKRQIGLLRGLVGAVTSLSFSGDGRLLASADESDTGLVRIWDVGGASVFAPLECSPNSGAGGLPLRHSVEMMSLSPDGSRLAIVCADPSKENVRVIDVFTGRRMAVMGPAGRGGHDNKITAVAWSWDSSRIATSSDDLTVRVWYVGKPTAPPGNQLAAAAAIVAAEMSRDATPPLLPATPTPPPETSSSPSPSAPPWPRNRTLDEDMSASRHLVKLQSRTPSVVTDADDADGGGAEGAVAATAATANPAAAAAAADAAVTNGEIVFRPGQCQLILEGHHRKVQCVAWAHNGKKLASGGYDQMIRIWDTSTGEQLNLIYQVSWVSALAFSPDDARLVSGAGCRAVYMFNAATGKNLAGHAEPLPADASGGRTEVAGSRPATAGIAEVKGEGQGDEADPTASDSWIVAMRFNPDGTLLASRDMHSTVTLWDVSGMRLRPLRTLDGNFRDALFCPTSGDLQLYDMTASGALVMSAIPASSLPLGRAVQRAVTLSHPLLEFRGVAVDTAVNHNGVVLLTADNRAFFFVNGAHLAAVRQEASRAQRTPGSGSGGGSGGGGGRGWSVLTFGGGEQSAAVSRIPSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9J5</accession>
    <name>A0ABQ5S9J5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dihydroorotase</fullName>
            <ecNumber evidence="3">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_010103</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01294">
        <property type="entry name" value="DHOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00219">
        <property type="entry name" value="PyrC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004721">
        <property type="entry name" value="DHOdimr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00856">
        <property type="entry name" value="pyrC_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137:SF1">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="8">
        <location>
            <begin position="67"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="400" mass="43002" checksum="2052EAB6B9E13755" modified="2025-10-08" version="1">MFGVAARMLKTSFRCPKGCKSSFTACAYSTSRPPLTKAPLAAAAAAAAAASAAADALPSLTITRPDDWHLHVRDGQPMRDVVPHTARHFGRAIIMPNLVPPINNTQAALAYKVRIQDAIPSGSPFTPLMTLYLTDRTSQEDVYRAKEAGIVAFKMYPAGATTNSDSGVTDLNKPLAALKAMAEADVLLLVHGEVTDPHVDMFDREAVFIQQKLLPLLDQVPELKVVMEHITTKDAAEFVASAPPSVAATITPQHMLLNRNALFATGLRPHNYCLPILKREKHREAVMAAATSGSPKFFLGTDSAPHAKHAKESACGCAGMFSAPVALGIYATAFEKAGALDKLEGFASFHGPDFYGLPRNSGKVTLVRRPQRVPDSYVFGESEVVPMWAGEDVPWSVLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9X3</accession>
    <name>A0ABQ5S9X3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRO1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010488</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000037">
        <property type="protein sequence ID" value="GLI66646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="611"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="611"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="629"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="689"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="712" mass="74179" checksum="D8F926B96CDF910E" modified="2025-10-08" version="1">MASMNNAFTLLTGAEQQLNSKKKNKKPVANGHTTQSVPAPVATPSPSAVLVPPVSIDLVIGVNEACAIFERAAREAKTQGDKVKLWKEWTRLANDKTSKLKYADVDGVNLGFKQVILRCKALEICAESCISTPLHADKESILAQMFSSFLPSENGACYTLANILLRLSNSLSQDALDTLGAAQRAVNSLVQALKSAAEQGEAEVEAANPVAAWLSRVSALDKEISRQQALLQGLGAANKNVMTRETASTARQLVKLYEEKFDLLQPEAIPAVKPPSGALGVCLRSIDELKAVIGAHLKEAESKTLGTASVDASSRAAQIASYKREEEILATHAAQVATQIRTLEAQLQSLHAQASEIEDKRAALAARQRTVWENGNGAGSKGGRAQAGPAALSPAHYRDALGLADVLVGLADPRRLAAASPDQILAVQTAQVNCPVEYVAAAEHLMSMALGALGEVPAKLAFFRQRLAQASKLTQLGVVADKIREQSKIYSEEAEKMMAETMRLAEEMVVAATQARTDAHKRYDAMVRFNPEKAAVVAAHLRNAEALAAQIQQQYNVVLGAANGPVSLAPEAAGTAGVPAPATAGAASPGYAPLAGAPIAPVSGPVVAAPSPVPATAPGPRAPAPPHATSPGANKAPAAAPTPVPVTAAPSRPVPAGPPPRQWAKVEPPVAAVAADNSLPTPAEAFKGPSTSTGTSEQDGFKTAGKKNRRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA61</accession>
    <name>A0ABQ5SA61_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA polymerase</fullName>
            <ecNumber evidence="12">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">VaNZ11_010151</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="12"/>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05776">
        <property type="entry name" value="DNA_polB_alpha_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05532">
        <property type="entry name" value="POLBc_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3200.20">
        <property type="entry name" value="DNA Polymerase alpha, zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024647">
        <property type="entry name" value="DNA_pol_a_cat_su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038256">
        <property type="entry name" value="Pol_alpha_znc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045846">
        <property type="entry name" value="POLBc_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015088">
        <property type="entry name" value="Znf_DNA-dir_DNA_pol_B_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861:SF1">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12254">
        <property type="entry name" value="DNA_pol_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08996">
        <property type="entry name" value="zf-DNA_Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase alpha catalytic subunit N-terminal" evidence="17">
        <location>
            <begin position="21"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="15">
        <location>
            <begin position="571"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="14">
        <location>
            <begin position="912"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger DNA-directed DNA polymerase family B alpha" evidence="16">
        <location>
            <begin position="1464"/>
            <end position="1678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="69"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="940"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1362"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="943"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="956"/>
            <end position="972"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GLI66368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1704" mass="179424" checksum="DA4199204F7314CD" modified="2025-10-08" version="1">MDRSRRQLPQGSAAAKAMSALEQLRAAREGSGKRALTFEVKEEDAVYDVVDEQQYADIVKKRRDAGAFVIDDDGTGGYADIGEDDFWNERPDGEDGEEETDEQGSDGKHANKKRKKEPCTAHSKRKHGGGEDGVSKPEGGNITAMFRKARAAIPGLQRSSAPAAKPTDASSDDLLESILGNISAAPPSLSGAALRNTTPAKVAMGAYGSAYGGAVSARAGGLGAVAALTSGAVAAAAAAAVAPRARVAAAAAATTPVGFKDEPHYAGEQDEPMLEAAEATDPPAVCMPVKEEDSEPKGMDDQAAATATATASAPLLAAPAGGSGAAPVAVGVASKGPRNYVDAPATPLDGALVQRAEDVWGEMFADGTVVPGGADGDLATLPSPLPDGASSVTCGGGATGAEGGAPLPLESNGMMPFYMLDAYENSDARPGEVLLFGKVEAEGRWQSCAVTVRGLQRCLLVVPRPEVFEDRDGAIAALEAAVKADPSRRVELIKLLQERCSSVRDEVRGLLTRAGVSSLRMMPVKRNYAFESKEVPHGEQWVIKVRYPAHMPTLPSPGGGGAPSGRTFLAIFGTHQSCLEALQLKRRMKGPGWLGICHPQRVDYANQATWCKVEVVVDSAKKVLSGEKGLSADAASRKPPPLVVASISLKTQINPTSHQHEIIAASVVYLSGVSPDAPLAAKDWSSPARLRNFSIARRLDGQAWPHGTEAAASAENATPRGRANGGNMVVLQASERSLLTCLLAKLQALDADVLVGHNIAAFDLTTILTRMQHHKVPLWSRIGRVKKTEFPRMTGGGHTFGGGAGAGVMSTIAGRLLCDTYLSARELVKSVDFTLSTLAANLLGQTRSDPLAGMAVAAGVGGDVAACFTTPNGVLQLLRHGESDAWLALGIMFHLSVLPLTKQLSVLSGQLWSRTLAGQRAQRIEMLLLHEFHSRKFITPDKQTYKDKQRKQQAEADAEEDGGGGGDDDGEDGGGGKKGGKKGAAAAAKSNGPKYSGGLVLEPKKGLYDKIVIILDFNSLYPSIIQEYNICFTTVQRPEDGSLPPLPNPATAANGLAPLPAVLQSLVNRRKQVKAAMASERNPLTRQQLQVRQQAIKLTANSMYGCLGFGASRFYAQPLAELITAQGRSILSSTVDLVQGAIGAEVIYGDTDSIMVATRTDSVEEARDLGARIKREVNKRYKLLEIELDGVFRSILLLKKKKYAAIKLEPDAGGKLVEVMEQKGLDIVRRDWCPLSKDEGNFALAAILSGRNREEVVAEIHAHLRQTAEKLWAGQVPLGKFIITKQLTKRPEDYPDAKNQPHVQVALRRRAAGKRDGVLPGETVPYIICVERHREGGPTDGLVTAAAVEGASGDGAAAAAAVPSSAGGGGCSGSLAERARHPEELRENSSLAVDVEYYLAQQIHPVVSRLMSPIEGTDAAHIADCLGLDPARYRGASSGSGGGGVGYDDDDSTLLAGSSTGTLMDQDDNFLSCSPLLLTSPSGSTFPLRGVDEVLRGAVLADHLLTPPDALGDDTARLGPAALVNQVLIRAREAISSYYDGVLRADDETAEVAGGCRSAVLRVAVPGGGGSAEDGPAGPDRVAHPDPARAGVLLRRVVGERDLYLQLCHYHRLLHVDGAVRRHFSRIKAADPKSTVTIEDLHAMIPESLRRTLASAAEAVDSLRRRSHWHWVDLGRLFGCLEGAGGIPAGLGPINSSSVRGVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB33</accession>
    <name>A0ABQ5SB33_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011310</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Magnesium transporter that may mediate the influx of magnesium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF31">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="472"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="516"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="81"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="397"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="541" mass="58824" checksum="063C9DEF45BBAF51" modified="2025-10-08" version="1">MSSSRRARSGVRFASEDEDAADAVAITLGDPVGGPLATNSIIDDVAQPMQNMTPAETDGEADPPVSGLHLGGLQMFQHAMSNDEGTSRPDSDADGPLDVPLGIRSTNLDPGATQAANSGFEVTGVEGGLGPAPVGGGGRRHHRAVGGKPGKSGKSNKQSTMLRTWLRIDKNGERSLLQADKWRITHKLGIQTRDLRLLDPALSTTYPSAILCRDKAIVVNLEHLKVVITTSFLLIINPEDEKVSKFIHEVTSRLAPSGGAVPQSRSYQSLTDAERLKLAPGHSTLGLDLPFELRVLECCLDVMAGHMDFLTQELEAGAYPALDALANKVSSPNLERVRRIKNNLVRLTTRVETIREVLEKFLDDDSDMHDLNLTAKELHEQEEQRLLLQQVNNADARSTASTTGSRSSGSSSSSASSDSSEEEAETAVVEMLLETYFMHVDNTYNKLQTLHEYIGDTEDLVNIKLDQHRNQLITIDLILTACTTVLAMMTVVGAWFGMNLDSGLQEAPGVFTDVALYSSVIGVTLLLLFVFWLWRAKLIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBH3</accession>
    <name>A0ABQ5SBH3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010772</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047046337" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="42"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="747"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="769" mass="79408" checksum="CE25B520DD344087" modified="2025-10-08" version="1">MIRYRCHLIILGALLALRSAVTQAPASVTTAPGFAHCALMPNECSCSPYRIQPMERAEVGNVVRACFQVISIGCDISLPCCSTVMVATEKLTIGSTSVCVSANIVNVTINGALHNSWVTTTTMLGSDLTITGLSRISSGLPGAEFCITSQRPCWSWSDLCSNQMTRSCQYSFVAGVFCPLCTISLPSAPDEQLAPPPPPPPVLVNQPPKPLPPPRSQVLMPPPPPPKAKKSPPPPPPPTPPFSPSPSPPLPPRPSPSPRPTVSPPRPSPRSSRLPPPWPPPPPPCYTEIYLYALPPLNGADSYAFDAVTCNRLTALITLGISSQAAVTGATLLQPFQLHQCAGMSVSVNGTFRSTADGALLKSWLDGPNGLPAWIAALANGGNEDGRCIADLYGHTLLGTVGSTTGGRGGGCLYGQYNMECSVPPPAAPLTPSISFSPVAPPPPKYPVPSSPSPPIPPPPPPPPPPPPPPPPDLPLPLLPPDFPPPPSPPPPSPPPPSPLPPSPPPPSPFPPSPPPPSPVRHSSPPPSPPPPSPFPPSPPPPSPPPPSPPPPSPPPPSPPPPNPPPPSPPPRRPPSPGPPRPKPPVPPLAPPSPVSPPPLPPPPPFGILVALNVTPAPSTALDVINITKCTRLSSLIGNAFADNASAVGAVLLQNVTLAACRQDSIVMRGVFRSAADASLLQPWVSNPVVLSSWVYDITGGQDCLLMDRTKGYVVVATVAGANAAVGPTAFLRVAFSTSCPAAAAALDTLPPPKRRRPPPPSPSGTRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBM1</accession>
    <name>A0ABQ5SBM1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011491</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.210">
        <property type="entry name" value="Chaperonin-like RbcX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038052">
        <property type="entry name" value="Chaperonin_RbcX_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003435">
        <property type="entry name" value="Chaperonin_RcbX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33791">
        <property type="entry name" value="CHAPERONIN-LIKE RBCX PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33791:SF1">
        <property type="entry name" value="RUBISCO CHAPERONE RBCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02341">
        <property type="entry name" value="RbcX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158615">
        <property type="entry name" value="RbcX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="187"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="301" mass="30876" checksum="2421D27C42F92570" modified="2025-10-08" version="1">MRPMWVFCGAPAHGRRAGLVARTVPAVRRDVRAKGGVYVPSDSFGGQSPERKAAGILRTFFTFIAARIVLAQLEGSGRGALGAYDSQAATDLHNFLQNVAMKDGDEWLAQLMRQNTSLGLRLMEVRDHYCDEAFEWGQLQQVSKKDMRAANTKLMRQFAAASLAASLAEGEDEAAASSRSLAGWRSAAANGTDEDGAADSISSSAASPDLSAMPTSLEVHSGLGPAGSGSGGGSEATSNPEPPRVNEGVTASSGTGSDATGSTGNGAETLKSPTPDTHLDGPGPSAGVPQAVDEASGTTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBM7</accession>
    <name>A0ABQ5SBM7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Xaa-Pro dipeptidase</fullName>
            <ecNumber evidence="10">3.4.13.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Imidodipeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Peptidase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Proline dipeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_011402</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Xaa-L-Pro dipeptide + H2O = an L-alpha-amino acid + L-proline</text>
            <dbReference type="Rhea" id="RHEA:76407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:195196"/>
            <dbReference type="EC" id="3.4.13.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.9" evidence="10"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48480:SF2">
        <property type="entry name" value="PEPTIDASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="16">
        <location>
            <begin position="71"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI67184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="540" mass="59389" checksum="DC14A783CF0928C8" modified="2025-10-08" version="1">MLLRQLPRFEAAVACNYSGKLPTVVNRGHTYRAWASHQSSILTAGTMANIGIDSPIEADTYYMGPGTLRISRKVLHGKLRGRLLERMQAVLPEEKRGWVFLEGGKEPHLYSSDGEPLFRQESYMHYLFGVQEAGWFGAMCVKSGRTLLFMPRLPESYAVWMGRLKGPEEFRKKYGVDDVHYVDETSDVIRRLAPPCLHVLSGVNTDSGLAVQHPAAPHAETPLEKDTLFDIITECRVVKIPEEAEIIRYANRIGSQAHVAMMRFARPGVMEYQLEATFLHHCYLTGGCRSPMFTPIAASGTNAAILHYGHAGAPNDRQTAPGDLVLMDCGCEYYVYGSDITTTWPVDGKFTTQQRVVYEAVLSAQREVEAAMKPGIAWPDMHELAYRRILEGLMAAGVVTGGSVEELIAADIGALFMPHGLGHFLGLNTHDVGGYPPGASPRSAKPGFRSLRTARVLQPGMVITVEPGCYFNPALLLPALEQDPAKAKYLVRVAVLSYMAVGGVRLEDNVLVTETGIERLTHVPRSPEEVEAVMAGAPWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCX2</accession>
    <name>A0ABQ5SCX2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="28612" checksum="11B705423B2B56C5" modified="2025-10-08" version="1">MRSSFACKRLAALGHQLSAFPTNRNCLPEPNSATTWKLVPNAFPAPSSSQPASFLRHQSDQTAGTSSSWGQLKKDGVRVNPCQSPHHPAAVEGVAEPEGEELCVQEAYNPESKCFGCGHAHPDGLHLQSRRIPGLGLGRLEACISFDPKYCAFPGIINGGVLTTAMDCHGNWAAAIALMDKGCLPRPPLTMTASMQVSFRAPTPPDTQLVMRSRVLAIKENTSGGPLKATVEVEVVVVHPEQGPAGEALERVLAVGTGVYKRLGALRSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD77</accession>
    <name>A0ABQ5SD77_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyridoxamine 5'-phosphate oxidase Alr4036 family FMN-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024624">
        <property type="entry name" value="Pyridox_Oxase_Alr4036_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28243">
        <property type="entry name" value="AGL049CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28243:SF1">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE ALR4036 FAMILY FMN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12766">
        <property type="entry name" value="Pyridox_oxase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase Alr4036 family FMN-binding" evidence="2">
        <location>
            <begin position="18"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="212" mass="23819" checksum="BEB5D1D77B240686" modified="2025-10-08" version="1">MAFAAEVAAVAGRLSTPIWRDKIQKSLNKNKHLANSRYVQLATVRQDGRPANRTVVFRGFLADDDDSLTFVTDIRSRKVQEVAVNPAAEVAWYFPQTREQYRIAGNLVVIDAAHVDNALLEARGRVWSAMSESGRQQFGWPHPGRPRAAEDPKAWNNPAPGSQDPPLDTFCLVVLRVDEVEQLLLRSNERFRYTHAIKEDGSLAWAEESINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDA0</accession>
    <name>A0ABQ5SDA0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011877</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="400"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="779"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="969"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1070"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1169"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1235"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1355"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1443"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1489"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1745"/>
            <end position="1772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="783"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="794"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="820"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="866"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1827" mass="189549" checksum="565F023865EE9E77" modified="2025-10-08" version="1">MENSIDWFDPEGFALTACLDERSQPLRWRVVAQDLLYSRPGAEFPDPAQCESSTIISTVLFPPSQTVLAAGVVKYHGLCAAASPRSVIVTASTQSPDDINPRDTHQPLQLSLQQHRAVIHVWELRIRRRLSVCALSPAVPPSDTNPGPCEPSHAHYSADGDHSKFGANLGSKRLGLTLTLHEVTGMPLPQGISSAGFVCRPGGAGVGALHHHQKQQQVREWDVTNGCAPAAGGGSWRCSLAHTGAWAVLQVSSTRTLLIGFGAALEPTVVPVPHFPDPVAGGLGTTAAPAAPIAAGPGSSVIETTQHQIQLPHQDKTTNLHCLDLDLTAAVAATSGESSSFHVCQVLSCYGYAGQVVLDVALTANARRRANSDLDDNDSGGSSDDNVGGHSVLLRLGFPVKPISGNSRSGRENEPTDDVAQGGGGEVRLVRPTWGTRAAVTASVQLRGQISICSQLHSLAVSGSRRTKLGKTSPTGSIGTSSGAAIIDLPALIGVHACGHLYLIGPYSRTVRPGVDMDVGEIYLDRGTLKAAALRSERAPAGDGPAALSEHGPTPPPLALAVVAQLTVPGPVISIHPLPRIRMSAMTGSSIVNADGDTNGCGVDRAHRGQGHPGHGAELHQIARTAAKAAFAAALCTDPARSVVVLRVMRWQNTEDAQLAARLTEATAVAECEVTPAAGAAGAGGPRPAVNADGPYFQWSLQQLLIVPGVQAVLPVPLPSALSLALAPGGSGYGIKNDAGADADCADRLGASGGDAVRAVRDSGVAVLVSPAAVLILGGGSRGAQEQGPARRGAAGGADQGVGHQGGRRSSGDVWMRDEEVVEEDERMANGEEDWGPAEGSDDDWGGGVYTQRQCLHPPPERRQHGQLWQQQAQQRPDEEAEEERGGGGGLTALGPVGLGPWRRVVVLPAEVQPLPPQVLLAPGGGAHAAATKPPAAAAAPRFAAGAVTELLLMDSSCDTPPLDARPMQWGRPEQEHAQQGGLGSIDGRSGAQEAGMAKVLAHMSRRWDAGVQHALEQRAALGRMVHLTREAEHLIAAAGQEPLPTYGSAGARPPAHASLLDPLQDLEEEFRSQPQRRARSRSAMSKCGAAGPTATCSGRGGDGDEDGDNVNVSAAVDKLLDQLLGCGSAGPAKVRNIAGAVPAVAAAYSRSESRTAAMHGAAMAVTDGAKSGGGEGGAASDPRMPDNGSWSPVVESLTQSRQGGNWILRAVLRLEGGQKAGAGYFAAVAKADGNGKDDGSGSGHGVGGMILPLASGPQHQPPTPPLSPLQLDDVVLLPYCPAGLALSGVRGRWEVLQRRSRQVMVEAAVPLGELLGLASAAAVTATPGGCLDPGFGAERDFDPESHHRRDLQTALGPLPGLFDGPNEDGGDTVYSDECSSDTDNDREDACNHDDLRLHDYAGNGIIGSRPAHGNSSTTRRSTHCKLRELQLGAMLRITARVSGSRTLEDGGGRSGGSTESSSARLLQVRVLDLGRVLVPLDSLLQSATSGSLRQQVQQQQAEGGRKDASTAGAGGSHGSIGAALQPPPGPAMALLLAPSRSLLLIATTEEAAGLKVRMPPGVAPVAAAGGGKGLPSCASASCRTEVPQWTRGQGMCGDLWERLLWAPGFLTRSEHDSAGVPTSEAAEDVEDHAACRRLLWEGLMGWRCVVSLQPCGVYGCEVELSCINGGDTGWDAWSAAALDQVQQLVTEAAERSCEQVGRGLSLLCPNPLADCQLRRMDTAAGALQRCVDATLDLFEGALLAQQQQQHLKQQQQQQQQLKQQQQTAGRREVGTKSAVKDCTAGSVGSQRVLSVGATGGRLQAMLQDVLQLQLQLNTAMETYLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDE7</accession>
    <name>A0ABQ5SDE7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_011534</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668:SF43">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14, 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="197" mass="19405" checksum="CC9EEAE9584D671C" modified="2025-10-08" version="1" fragment="single">LRSGRMATIRFTASAPAPPRTLIRCGIICPLRRSFLSRRVFAYDVPGMANAKSVTGATRALANDVGSTGGSGSGGGGGGGSSGSGGGDASSPQPHPMRNFTFAFAVLLAVGGVLALARKGSATSLGAGGGAALILALCARSMVGAAAVGPARVAFALCTLLGVVMASRFNNSKKFFPAGMVALLSLSMAVGFVVVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE06</accession>
    <name>A0ABQ5SE06_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_011794</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RPGRIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021656">
        <property type="entry name" value="C2-C2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031139">
        <property type="entry name" value="RPGRIP1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14240:SF1">
        <property type="entry name" value="PROTEIN FANTOM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14240">
        <property type="entry name" value="RETINITIS PIGMENTOSA GTPASE REGULATOR-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11618">
        <property type="entry name" value="C2-C2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="320"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="356"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="416"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="1107"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="501"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1259"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1364"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="50"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="180"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="543"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="679"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="861"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="501"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1262"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1306"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1364"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1435"/>
            <end position="1447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI67567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1805" mass="200513" checksum="BF7E02B1FCEAD83C" modified="2025-10-08" version="1">MPNDNRGLDGLDESATSFGSLPVPKVARYAWLPTNDKVNNENFHKMLEEYRATKKRERELELKIKQLGAQLARTEEAAKRALVQTDASAKGGAAQKLLDAERTIAKLRAENAELASKLAREKKRSAEFKTLADTYKQRLDKFLRDQRNLVKRVGELERSQAVAKKRPEDDYWGEEAGRRFVAQQDELVALREENAALKRLMESDGPLAQCKAYEGQINELQNLVKFYERKLAGLAEAGLIGPEALAGLKNQPGPDDWVLEEFWHNDEMHLLDRKNGKLFTVPGDNNWPRPIGMRTNMEIKMGIHNSMERFLATLDAFLTNNAARLQAVFAQFDLDNSGQLDRRELARMLQTLMPDLNPSQVEELRTMLDVDGDGLITLEEMLECIKEAYAARTAAKLGKNIEVNDALDRIREVLRVNKQAVKDSFDELDTDKDGCLTHMDIVRLVRPFLNDMYQKEVRYLLAKLQQWDVVGDSRVTFDELYQVMELVRIFRVGPGLGGVMRTSSPLRSPPRTVSPTKLRGGTISSAHGLRPSVRSTGGGGIKEAFMRERVTQLEAELRDANRRNVDLEDGNKKLEELQRDVALYKARIEDLERDFMRIDLLGNMEGAAGDEQLQKAWEIASTFKKRFMEHKGELDNIRLMYARMQAQLDETHKLLHEEHRKRFKLEDEITRLNMELMRLGDLESRLTHEKGERVKLEREYLALQQKALNAPGEALAEVRALREELFAVKREKATAQQKEAEVRLELSHVRALLDGMNLQSYRTMQDESDTLKKRIASLMLELQAARDKLAVYMRTGLPNSANEDLLLEDDDLLFGSDKRPDTDKTPEELRRELMQLRDVWRLDQGEIKKLHKVLETESAITMEAKAALEEAHREMERIKRELQADLRKLERELERRDEKIRKLELQLRGAYSGINRALRSSRRGGADGLRDSLRSDTDDFSEIGEDQNIFELHITEAQIYEEALGKDPSVFFTFDFFMHETQATPIVPSNSPSFNTIIQYVIDNDPFLIEYMDTHVLVLELCRARGYDYDVLGMARLPLRQVLEDLEIGAALGYNRAYHYADVFGADGKRLGRVRYGYCFRRPLDSLLKEFRLQTRTKRASDPDERDPATQAVQLALTQPGNNSCIKVIIERCEQLIPAGGTALTIKPYVHYKFPGTRDYHDTRTLAGTNPVFNDEAVWPIARTPELEVDLRSRHLAMVVFDDAQSDDPLRAIVGLASIPMTSLANGIPVEGSFKLTNPVTRKPAGRVVLGMGWHNPLQLPGAPPKSPPPRVPISSELQDSGPLPDYGLSSDVQELLPVRGGRAGAGTTTPTRPGLSLGASYMQPSSTPPRPSDMHGLAAELGAGDLRAPSDQGYGLVAEQRTQQQQRAGYGYGYSQPQQQPRPGSGFGGFGLVGDTAGRQPPVPTPNLGVLPYDRNFGLDADTRAADYPPPQQQPLGRQPAPLRQQSPYVHAPDGPPSSAISSRPQSEYGLSAETAPPAGLSVPTRMGSFGRPPLPVGSPAPAASRFGPGSVTSGSRRPSDGLFGLDAEAGSQRGGQGPGAQPLPLDEGFGLGAEVDDGGASSSPPQGMILPGRGSGSGSGIGRGRYADAQMSLESSAVPHMKLQQPQEGPLALDAALGPVLRRERPDPASWNGLNHRLVLCLHSIELTPQALRNPRLRNVVLMHSLLSEQEGISELDTCTQPVAKGPGQLQIGYCVSYNVLAGTATSELLDALVGGDDFTLEVKLMSTAASRLDMNSIAQPGTLQTLGSCLLPLAEIWSGRRGDLLQERVEVLDDSDDITGVAVLTISLMAESALRTLRQQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEB8</accession>
    <name>A0ABQ5SEB8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4042 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="360"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="540" mass="55374" checksum="9B9F65A578B88602" modified="2025-10-08" version="1" fragment="single">GKMPRLVADLLAYPAQLRQLVSEGMTLLWEAVASEPVHKVVRRALKALGLLHALSNILTATDTNRAPDRSNMAVPIMSLDSLTGASPEIARSLTVTLPTVTPAEAAGRWASHPMGCPCCEGPPQATLAASAAADLTAALSHVTLVRRLLTEGCNDLEARCEALRCLGRALGSAMLAAGAAAAAAKKKGSDGQLAKTRSAGAVNAVAAAPPPLLAAAGSFLEQVATATQPWQPEDWRLAGAEALAASRLLQSAVRATNNDGVEVPPPASLPLPPLPSQQRQPSEVVPGGSPGSLELLAIRGWRCVIAFLEDEDHAVRYTAARLAQKVMELAASPDDNSPTFHSLTSGEPSLPAAGGNSTVAAPAVAASRSRSSGRSKNVGVKDTGKGAESTVYGRQPLYVEAVIRRVYPWLAAQYGRCDDGSGGGGDNSDGGGKTVVTRTALVEVLCELVSGSTDEDGALVPEIVATAAASAGAAATAGWPSQARRLFDREADNPHEEPLLTAQIAARTLRALLAAPQQLLLTANGGSSGYCPGDTGSVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEC6</accession>
    <name>A0ABQ5SEC6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012653</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="153" mass="15514" checksum="EE4B5A373EC88BCF" modified="2025-10-08" version="1" fragment="single">NTLKRRVKFVLLVNSGGSVVLISTPLSAPCQVMSYLQRFCQGSSLRHAAAAWRAGSAQLQAARVAMAASQRFNAAPPAYVAAPNSVPSFATSPSIPLSFAASGVQSTAGVAAAGTSGAVEGSDSGEAGNSMSGASHTGSLSGSKSLRKRRKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEE5</accession>
    <name>A0ABQ5SEE5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012658</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF13">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046573498" description="Methyltransferase FkbM domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase FkbM" evidence="3">
        <location>
            <begin position="330"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="705" mass="75661" checksum="1E21F832D301470A" modified="2025-10-08" version="1">MLKSKLLLLVLLLGTSSPPDQSRTQQHTGAPATEATTFGKGASCTSCAHLHRSPHLRSRQPLFEHNWHQHHGTWTPSQWLRPGVSRGSSFGHSSSNGGYWIFKCSSAVSSRFMGASRGWGIRVATASLVDAGGVNVDGSGLRGDRQAASGISRSASASAAAEKERPNSASGGGNASSGTGAADGASNVCSDSCHLARNGICEDGSNIIKLARDGGVQVACDFGTDCTDCGPYRGIVSTWGTEGGFKGPIDFLRRQGVKVYARRVTFEPRFLFAYTDPAVDLDVSEQIERAGCMELGIVQVFRHVLSERCAPFKVQTASSEAQGSPPLVVDVGANFGYFALYAAMLGCRVVAVEPIPRFLAFLHWNMAANGIKVTTQAWAGPGPGLSASPGVIVVERVVSTISSQNISMAAPRKGVWGTASVAGMANNVDQDLEILTVGSTHLDEIVGAEQVLLLKVDVEGYEPVVFQSGQRLISEGRLENILMEYSPGMPETLQRFFPTVGTKTPITVEDPPKMLLEIMNKGFTIVQLPDNFAKSGYSPQLLDPIPEFQEVTARSVMYDLVDAAWFQNISASGSKCPWPPELQLANAAVWGEGCLKAPEDSHPKGFRVTHSYNTNLWASRNRSSFKISGPVGLFAPEQDETKSWWSQRYPDKGVGNRACAFLPAGQLLRSRCRCSKPDVCGREEEALLKALATGQMPRPPAPPRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEH1</accession>
    <name>A0ABQ5SEH1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012657</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040780">
        <property type="entry name" value="Rpn6_C_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040773">
        <property type="entry name" value="Rpn6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18503">
        <property type="entry name" value="RPN6_C_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18055">
        <property type="entry name" value="RPN6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="223"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="421" mass="46183" checksum="BE15E102E3465A66" modified="2025-10-08" version="1">MASELEGRLKTATELGTSDVPAALEQLKGLVLEDSNSDADAVRIKEQAITQLCELYIKQGNAQGLADLLTSLRTFFNAIPKAKTAKLVRSIIDAIAKVPGSTQLQVDVCRAQVEWAKTEKRTFLRQRIELRLASLYMQVKDYPAALALIGTLLSEVKKLDDKLLLVDIYLLESKVNHALRNVPKARASLTAARTAANAIYVPLQLQAEIDCQSGILCAEEKDYKTAYSYFFESFEQLSSLDDPRAAQVLKYMLLAKVRQDQADDVPGIISSKAGLKYTGPEVEAMRAVAQAYHDRSLQTFQDVLEAHKTQLVDDVVVHAHLASLYDTLLQQNLVRLIEPFSRVEISHVAALIGLPVATVESKLSQMILDKKFSGTLDQGAGCLEVFSPAPPDAVYPAALDVLDSLGRVVDTLFARSQKVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEU6</accession>
    <name>A0ABQ5SEU6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_012155</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF285">
        <property type="entry name" value="V-MOS MOLONEY MURINE SARCOMA VIRAL ONCO HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="124" mass="12862" checksum="E09940848949515F" modified="2025-10-08" version="1" fragment="single">PEHIAKHSDAFLVEADILANLNHPNIVRMLGVVTSPPAGDNTGDCNGGAATGVSDPVVGIVTEFVGNGTLGQVVRSPSGRLSTRQCARIALQVAVGMAYLHSQSPAIVHFDLKPENVLIQGEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFA5</accession>
    <name>A0ABQ5SFA5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="711"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1043"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1181"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1258"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1360"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="738"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="760"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="791"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="804"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="844"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1259"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1276"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1372"/>
            <end position="1403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1582" mass="163368" checksum="FEB7B8B632AC1AF0" modified="2025-10-08" version="1">MGDVLQSSQTSSDGSDNDGDVTNIAKANLSLLEVLRKSPVTPSAGNVRELVPTSRIPLPIKLNQHIESSHKHFVGHQSQFQSVAAPLLPGSRPSGVHSANPSLASAPDCLGRASGTLPSNHGAQNSAGTRQGPPSPSANECSTSSDGGDSDDGIPHFNILRSRSMHPALQATCRRHSVGNSTGPRSRDLARPAVNSSVRNPLKELNPFPAPDMSPAAAAAAVGSALATGGFTTRIGCKDGDGKISARAAASLSAAEPDVMVAPDVHEAAAAIVQQNPHAACSGGQAHLHGILSGSQGGQGGSCTSGSCSSDLDGSEEDEGEETVAVPLFNLLRRAPARVQAAAPPPPSGHAGGSTIPPVARAVGTASAGLPVSSGAEAAGSCPGVARLAATMLCASPQEFASRRRCELHHGRGGVAAALPVSPARHMAGGESPPQLQQSRKFGGVCHASHAVSDAATTGAKQLRLTPLATMPAPGADCGVIASAGTALATLPGSATRVPTAGRLRLGRSGGRVPSSPLPVATTPVTAAGAAAVPGTTNSGPGKLTDEMLARAIQTAQRRRRSAPAPLRSLFEEEEEERRQRPGRDCIAQDQNANALPSPREAAAGIGSPRGGGGGSGGSGCLLTNEGAHVDTEDLTAEAPPGQRRRITHRPRPTEELITIRTEANSDVEKDAAVWDLSPVDEPPNDAFLWDDHGAPGQGWEEAQECGWMQGSAMAPGCGQQRWEQEQKQQQQGGGQPLGHQQHQHQQGSSRYSTPAAAAAPPPLPHKSPPPESNRRQRQRGLSPLLPPQQPQSLPQQLQQQRPEATSEQQQLSERQGGLEPPLPLQPQQQQQPTLAGSGRHGQAAGPATKRRATAATKAATGTQNIMSFLLRQQQAGPASAATSPVDVASQKRPRLRLSAPDLGPSAAGALPTAASAVAVAGANTLKPVTIDLVDGDVSQPRRRIIHRSEVRRPTGPAPVIVVLDDDEGTTGAASAEMGGLNEAAGVKDPEAARLEDEDELPATGNAWRVRKHKRRHAVLLLDDEEEEVEEVEEYGGAGAVTAAARTTEPPRSLQGGLHPEAAGTCANNAPHVQPAASVGAMSGGRPHGYSLLRVPRRYEEEGDEDDVIEDCSQDHEEMLGLCSARNDARGNGHGGGGGGGSGFGTAMATGRPAVGRQSLGELVGGAGGGYGSSEAARRLSLPAGGHPLRGLSGHQLPGAAPQAHGGPGGSAAVSMGMALGMGQIIRGNAAELPRLQNGLPLQPSVGAAVGQSVPQHLQHMHQHHQHHHQPPSPPQQQQQQTYQSLQRSSSYQQEQDRQHHQQQQQQAAPWWTLLPDFVPAAALADGFDPRFKQRPEPVFVLYQKQFNGPANSSVAPMHRNTWQHAPPGSGPGPSGNGTAFGGSGGGGGVGSTGGAHGAGPSIGVGTDNEVMDWARYLRSGKSRCGGGACSMRKVAAAGPAGGAAADTDGTSGGGRWYTNNYGVRVSSRRDEWIVCIGKCIGCMGGGVVIGEWVNEGILRQQFHHFGWESSTTVNGYRESMENAGNIAPLLSDRVLTASCTVERMLHELNITSTKFGVVRVVLKSPQRSANAQELGMGALSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFE7</accession>
    <name>A0ABQ5SFE7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013104</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045151">
        <property type="entry name" value="DCAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574:SF40">
        <property type="entry name" value="WD AND TETRATRICOPEPTIDE REPEATS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="48"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="531"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="832"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="917"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1115"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="375"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="543"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="560"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="844"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="932"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1120"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1139"/>
            <end position="1148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1161" mass="122390" checksum="6329F412C33614E7" modified="2025-10-08" version="1">MSMATSGMHRLLLARERGVAGPQDALAFERSLHFPVHFADKLSIARVYEGHNGCVNRLAWNADGSLLVSGSDDRRAIIWRYPGVEQIPLALTTEHRANIFGVQFLPCTGDRKIVTGAMDDTVQLHDLEASPATVAGLGSSEAAAARAGQQGAAGGGMAIPRRVAAPKPVRVVMPRTKVYLSHKDRVKDVKVEPMNPHNFWSGGEDGDVRQYDTRMRNQDKWESPTVLVQVRDGHRLVQVKSLDINKAHPHLMAVAGSDPYIRLYDRRKLSPGTWKRRGGTPAVMLLAPPHLPLAAAARQGRSHATYVSFSNRGDRVVTTYHADHAYSFDITAAGDLTALFPQRLLACSRPLPRSGRFMSSWMPAAAAAAAAGGGRGGEGAAAGGERGGGVSSGVAPLPPRAEEAKARGNAATFGRKWSAAVKAFTEALYFAPAVPILYALRAGALLERNWVGDAAFALRDCDTAVALDPRCSRAFFSRIQALREMKQYQCALCAVDEYRQRFPGRCSGDLSKLVLQLRAAVEERRRLYEVRRQQQERRRVQRAAEALARTPRRMTSSSPRPAPDPPPPPREQPEQELQAAAGPSRPLVRRSAGLKRSAASSEVAVGAAPVPAGEASAFEVGLEQEGVERGSGTAIELDGPQSHNLPTGGGGGGGGEILVSGTPSATAAATATATGAGTGAFPAIAVDSPRPDAAVLQGGLPASISDRISPGRVFLSTNAADADADAADADYLSITYAATTNGLGAADARRTRAGEDTVPPSAVMAAMNEVLAAVAAEAAAVAAAAATDQRLASSTAQGEVEDSLELAAAAAEEEMEECVEAGMRLVDSMRRHAEAAGEQAGGAEESEEDEEDEQDHEEEEEEDEEEEEEEERGGGRSDSEYDEEGDITSREGDGREEDPDMEPFLQFYARAVELAGSSGCSSTSDDRPGLKCAGGGGGGGGGSSAAAAASSRALLGAWSGSPGGRRMLQKYLGQCNVQTDIKEVNFIGSDDRVVAAGSDCGRVFLYDADTGALLHVIGADEEVANCVQCNPTLPVLATSGIENVIRLWSPRDALPSTAAVSELVDDFANVVEKNQARMREGPSIFRTSMLHDALQDNPQLLHLFMSHLYGRDAAGREASHGGGGGQADRSAGGGRGEGREDDDEDAGEGEGTHPEVSCRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFV9</accession>
    <name>A0ABQ5SFV9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dickkopf N-terminal cysteine-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012644</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045672223" description="Dickkopf N-terminal cysteine-rich domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="48906" checksum="7919A37EED9E2948" modified="2025-10-08" version="1">MHAPQRAFLALLLTAVLAAPAFAARHTALLAKTEDNLSRPDDCLSDDDCKQAEICIKGECLLLKGDGETCRHDEECRSGTCQKSWWPAGAVCATPCSLDEDCTSDLPICIKAPYMARAPGARAGDAMAFSDKTASSTAAADSNADPAAEEANAPTSEDDDDDDPNDDKDDEDQDDDEDEDDDDDEDEDGDGDEDEDDDDGDEDDDKDDKDEDEDKSTNRHHEDHHEGHHEGHHRHDERGKKAKKAEKETEPSEAPADGEPAGAGSADGAAYTGDHDHDHDHDHDHDHDSPGAYHHRHPHDMATSEQPLNSGMRKASTRWTGVSSARTEVSPVRILLRDGEKVREKAAGKDDFKSNGGKHDGQQMGHCVQCVNDKDCAEGYYCERGFRRNAHSCVALGKECGSYNDCDTGEYCEVTSKRCRPYFLGDVVKGITKIAKVEASQTVRDARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG34</accession>
    <name>A0ABQ5SG34_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013235</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0157">Chromophore</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="122"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2194"/>
            <end position="2217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2402"/>
            <end position="2420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2432"/>
            <end position="2450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2551"/>
            <end position="2573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2761"/>
            <end position="2782"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="621"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="969"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1172"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1257"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1306"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1351"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1413"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1572"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1712"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1775"/>
            <end position="1909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2081"/>
            <end position="2161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2456"/>
            <end position="2518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2848"/>
            <end position="2870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1185"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1260"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1352"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1448"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1714"/>
            <end position="1725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1780"/>
            <end position="1794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1803"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1822"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1880"/>
            <end position="1894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2081"/>
            <end position="2091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="2092"/>
            <end position="2109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="2125"/>
            <end position="2134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2146"/>
            <end position="2161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="2456"/>
            <end position="2473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2870" mass="309468" checksum="CF675D3771B7970F" modified="2025-10-08" version="1">MAMFSSAASTSSRGSASEEGTTGPSRIKNVGSPDGDEERNDFEETHKIQAGIFSVLYILSKEKMNENLKFTVIKLMFDFTQLFALVVNPAHGWAVDSTSVYWQVLTFSRWNYIVTNLLGYKAYVAILYTMTGMLCCALALCMWIAYAYKNRSFAYTWPIYVLRLYATIHFGLFDIATLTLIQVSYDCQFLGSGKSNRGHMYTFPDKVCYKSPHLIPFIVGLATQVVFVIAAVIFFTGEFEVNPISRRFTCCAHSHVEVRAFVLKVTMTVVYIIIGWLQVQTVIQAFLCLALTWVFFKNMPYMFAVINHIRVGSYLAVTWTAVLAVAIMFKPKNPAQVQIYEKRITDVMLYGLAPIGLLGFGASFLRLYTWSQFVRKRFKEAPSGSKVKDIYRFKDPIEVEIISRVARHWIDDEVLDLDRVKEAEAIIKAGLVLFPTRAFMIMLYSNFLWDVLDNPQAGYSQLQAAKKANPNYMERFAIYRREQEHLARTAQTKGNGESTLDLVSYVEFQRNYRLAMRAHREALVATRNFWQYLLQQHITFTHLSKSLKAIESAIVKADKVYRTVLERYPYSAKMIKGYARFLEGVKNDPWRASKFYTEAEKLEAEREEEAAALELEGLDGDDSRLLNKVDERVNAVIIINSRGQIQMANKLAYAMFGYNKGELEGKNVAMLMPLPFSQRHNGYIKRHITTGRETVMNRVTDLVALHKDRYVFPFRLAVSKVSGAGDDSLFMGVIMGVEPPTDTANVYILSGGSVAAVDQAFVDWFGYTVEDYLGHPVHTLAVDPVPFKRLVEEVTRHDQPTEDGVCPVILGAKRVLIKHKYTDPVDVSLRLKATGLGTETIYRVEMRRNQPPVELVLTNRKGRIAFITSPLARALGHTPRSLRKVDIGELLPQPFGAMHASWIREAAESKPSSHSCRSGVTMVLGPTQKTQQTVRLSIKSTDESGEQQHVVKVSPSTLAEGLDERRLRLTIDTSGTVTDVGQSPNSLFGFKPSALLGRSLADCVDVLHVAARSASASAGIVPGSAGPSAADASAAAEVRRIITLLMGRVLERPGLSWRVGVMPPLENIRSLGSIATALLAKQTRPAVMELDVKVDLEDLAAGREVMINISLWRADLLCGVLEVDKEGVVLTSQDHPFYPSGLLFGLSSPSLLRQPMTRFVQLGGGRTIESLFSSEHKGSGKKGALKSSSAASKKVGPLRSLTAHHTDGEPLTLTVQTVRKEGANSRLFVVVHFKEPTRGSRDFLMLLQRGPVEPRLTQEGAETSPPGTATGAAAAATSTPPGTAAVTPSGKGGVAATLAAVLAADGSVGGSKSSRKRRSVDMGPGSGLSMAATVVAVSPRRCSSDLTALMMQAQQQQSGSRSQLTSPTPPPPPVVVAQRSSDSPLMGMPMSAANIGAPSLMAAAVAPPPPPPITLLKVSTGSGSGGSSGGTPSVSTPPKDDSISPTALAPPPPETGMQALPPPPPVPPPPPRVVTTPSMDQPRVVAAGRTALAMQGAVAAATTAGGGNRWSMPPITPYNSGPVEAVDTAIAAAASSGLPPGVVGLTLQDYLVGPSEKSEDDLAALFDRAVSGSGSGDGAGGQPRLHQRPSVRRLAPNMRPVGLSNLSFNRRLREKLAAEAAAMATGRADSYVAGEEPEDAAAAAAASVAMGATTSLSALMTLRPGSPVSLPPQSLPLQAAPSYPPWGVHHLAQQVPGAAAGLNALTAAVARATTPPPSLQPPLGPASPHSRPPMQVLLSLPASSTGMISPELPLPLAAAAAQAAGTMHVINVMATSHNSGGSGSGSGSGSGSGGSSRMSAVLPLPPPPPPLQPPPPPRNPSDLRTLTMQRSFSRDSVTSGSGLTNPGQGAPRRTNSTKQVSFAPSQLLIDETGKIERQPSLPQMGTPGRSNTGREGSVPTLLEDEPYNEYGGVDGVRGARLLTQSAAVPLQQQSYAANALLAPQPYGGMYGLPYGSAGGVGSIEEAFGAAGKIKSNVKLARIREWVLTEGERYFRLEDVQDIGDTVYPFGPDLLMRDDSRASHALVLNGGEGEGDVEGDDELDAAPSRWAEADGADDANDMESLDDEMVYSAMLLDSTSRDVMRGGGKDGKFGAGASSGGGGGGAQGGSDGDEHGGGSSPGGGDDGSEVGDDDDGSRSNHSNSTGQQSSPAAAAGVASSSAQADFRRGKRCRKLLKMLSTPAVQRATLAFRWQALLAIAVQLLANLACFVLLTVLLIRQTRGLTELNDVGQALVSCHDAMLSLQVMDNIYSNRARERSGPYTRLTITRLEKELKKALDASWYSHNDVYLGHNSLQHLPTAYNLSALWNDYNHQELLFYGPNATDQRSTTLWRLGNTFLRQGFELHHRHSELAKQLGSLAASDHYKYIHSNGLFELFHGYSLTLNGFVYQAVDTSNTINRLQLVLLVIEGCCICALVVAYMAWLLKKVDNQRYGMYGVFLIVPVGLVRGLASKTVAVEEENSDGEEESPEDQAGEELGGVMPQPHPPAGHGGEHGGMRINLDAGSGEPRGGRRNSRTQTNGLRSLYDRMAFWRHGSVRHGKRTMLRNSKESLWLLAPFVIWGLVVVIMHSLGYVTMEQASAPVAMTNVVNTVLTRAHRVVYFAQELAAADSEVAQKAIYPVLENEVLALKWEWEVMLYGANSTQATDPHFALARRGIAFEMGPATDTLFSSGVTCWMPDAADCYPPNHTYAAVVYRGLNAMMQRFFLEADLMLRDSPSAWQLNSSRLDYLFHEGTGNLHWAMMHLTDVHLDSVVALYMRVEVFHVVVFVLSWLLAGLFLFGMLRPFMGHTQRETERIAEMLSQLPADVDVEGLLNKALISIKGEAIAAHRGGDQRTGRNRIVPLSAPFEGELDAKGGPRPVPRVPSSVKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGT3</accession>
    <name>A0ABQ5SGT3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_013319</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-12 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044986">
        <property type="entry name" value="KIF15/KIN-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="98"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="483"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="557"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="681"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="972"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1170"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="183"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI68816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1276" mass="140946" checksum="71A70C36CF16B7E9" modified="2025-10-08" version="1" fragment="single">SWSKMLRGGDNGFTPRRLPPPKQSLSATQQDKGRADKTPVPLAASGLRSQSSGTSRKPPPLVTDSPEVIQRLESPSQPASVYSGDGDADGTEDFENDSVQVFVRVRPPTPQELASDKHEGCLAVIGSRTLHLTVKDKQNTIHSFTFDQVLPEETPQHDLFELAGQVAVDNCLAGYNSSIFAYGQTGAGKTFTVIGQISCPEKRGLAPRVFEYLFTKITDHENARGPDTVKFNCRCAFLEIYNETIADLLCPSANNLPIREDPEKGPYVDGLSEPPALNVDEMLQHLLRGAENRHTGETRLNRESSRSHSVFMCTVEKTVTSNGISKCFYAKLNLVDLAGSERIASGAHGGSNAAGEHFKEACHINKSLTALGRVTTELVKSQKNGGGGHVPYRDSKLTFLLQSSLGGNAKTLIIANVSPSSICAHETLSTLRFAKQTKHIRNEAKVNWTVQGDRIAMQREIERLRMELAGMRSGITEPLVQANEELTSRLEEERQRREELQQERERVFSALNQARRENRLLDQRLVGLAAISGMDGSRGAGALATVVEQLEALDTTTQDRRVELDQAQQELVEYQLQAEKDNGMRLKQELLITLSVRDDAIAKCTLLEAQLAALLKEKADLEESCSVLAENFAQAGAKISEQKDQLAVQHANLQEALGEVDELHGKLVCERHERETFERHKADIAAKLQAAAEEITQLSARESELQARLDDKQVELGHQGSRMEALERQLEGALSEVQLLQSRLAEAQERLAQTQHDNAASCSELSDQIMQLTFKLGAAEVQVSQERDNIESLQRELSSTRNELAVHVRCEDEVGELLKVKTEECSSALQRLAALESDLTARVEACGAAEAEVALLSTRLSQVQEQLAAAEAHCSEQAEMLSSRNQENADLLMQLSASTAAIEEAKQRAALAEKEVLEGTARLHALEVDVADHAAVNAGLQETLKKTEGVITHLEATVADLRVQLSSAQGDNAASEERAAELRSMVAGMEARTVELEVQVHALEAVKDDLEMQITKSAAESQDRTAALALLEARFAELEAVKASVIEQLRLEQANVAALRDALSSSEDCITQLRKQIQQEEARTFSALKEMSDTAAASQRQISKLEEQHSREVQNLKDELDKRSSALKSRDKEMVQKDFTLRNEIDRLNAQLRDQKIREEQIVVSNQIAIAEMNERLNRQTDRYAELTKKHDILKAIFDQSQRELESWKTKCERLDHALADVSRLVLFARNPPDSARSSQIMTRSDHRSLYIAESANVSPRLPNSRYHVSSPAGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH81</accession>
    <name>A0ABQ5SH81_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial inner membrane protease ATP23</fullName>
            <ecNumber evidence="6">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013163</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019165">
        <property type="entry name" value="Peptidase_M76_ATP23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711:SF0">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE ATP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09768">
        <property type="entry name" value="Peptidase_M76"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="114"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="313" mass="32520" checksum="88F9C5C705CBD442" modified="2025-10-08" version="1">MAHIDTKTFQSSQEPAALDTLTRAEVSEAVDQGLTSDVSVRKLLQATSEVGCSIDRSFFHVLRCSAAVGGGFAPEHGVILCNNRLHTRREVWNAMKHELIHAYDHCRSGGRIGSSASVRIPGSGGGPDAPLTSANDEGEKDRRTGVNRSRSGSPAAAAATDAGIADGAVKRTSTLDSDRESCNGGSSSSSSDSTSNSSSEGQQHVDAARVAARFGGLDWSNCHHHACTEIRAANLSGDCSLLQELLRGNLPLLPLGWAAQQRACVARRAALSVAMNPTCDGPDAAAAVVEEMMPACLADTAPFDPSEAVAGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHD0</accession>
    <name>A0ABQ5SHD0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013857</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="199" mass="21939" checksum="66809F1621A968AA" modified="2025-10-08" version="1">MGGDKLLDQIFNLKFTAKQLARSAIKAEKEEKAEKLKIKKAIEKGNMEGARIYAQNAIRKKNEQLNYMKLASRLDATVSRLESQAKMQMVQKNMAGIVKSLEKAMASNNLETIANTMTQFEKQFENLDLQTQVVDDVMGTQASLSTPEDEVTALMNQVAEEHGLELQHGMPSAAAGTKAAAVKQGEEDLAARLAELRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHS1</accession>
    <name>A0ABQ5SHS1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_013243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00164">
        <property type="entry name" value="S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057300">
        <property type="entry name" value="OB_Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057301">
        <property type="entry name" value="Rrp5_OB_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057302">
        <property type="entry name" value="Rrp5_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24682">
        <property type="entry name" value="OB_RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24685">
        <property type="entry name" value="OB_RRP5_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23459">
        <property type="entry name" value="S1_RRP5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="263"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="354"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="440"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="538"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="638"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="856"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="979"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="1162"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="1296"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="1387"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="1494"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="1585"/>
            <end position="1656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1657"/>
            <end position="1803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1832"/>
            <end position="1858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1687"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1742"/>
            <end position="1793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI68751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2149" mass="229367" checksum="3DD6F30E27B00289" modified="2025-10-08" version="1">MAKFQPQTRRRPADPKEGNMQRHERPPKQSEVAAIKSAAVGEEDFPRGGADTLTALERRELTAAAIKEVEQELADGKLPKAKRARITKSLQADAEDAFFSKHVAALEGNMATHVDLLRVKDLRAGTRVWGMVLGVTSRGLEISLPHGLRGSVAANQASDVLTLMLKAKAASEKDSGDRVAARPNKGAAILEAVGGAVPALTDLFAVGQFVRCVVAEAPPPPPPPPPSADNAERGKARRNKHVALSLLLRDVQGGMGSEALMEGLVLGAVVRSVEDHGYTLSFGIKGTSGFLRKRDYEEQFGEGLTLQVGGLLDVIVRNAADKRNVIVSCAPGDVAAAVTRESESLTGLEGLLPGALLNVKVRRVLRDGLLVSFLTFFHGTIGLYHLPALAEGTDWRKTYKEGAKLRARLLYVDPATKRTCLSLLPHLVALALPSPVPMLGSVFEDAEVMRIDGGMGMLLKLEGLPEGAAVGYCNVINALEERVPAEELVKEINARFKAGMKLRARVIGYRLLDGMANLTVRPGEVDAVVLSHADLVPGMFISGTVLSVPDRDGDGVLRVEVAKGIIGAVPKLHASEISGPAARTGPKAGRIKVKVGDSVDLRVLAVNPAARKLTLTLRKTLLKAKAAPLVSMDQAVPGARFYGVVTGLHDRLGVYVSFFADISGLAPHSQLGLEPGQVAKDAFAVGQIVKATILSVAYGRIKLSLAAKSKAAAAVAAVAEAPEGSGGDPLDGLQPGDIVDAVVLEVHTGASETTAADAAGGTAPTTPRSPFFVCHVQAAPGGAKVCAKLDEAHLSDHPAAVEALRAVVRPGTKLGRVVVLERLEHQSRVRVSRKPSLVAAAAAEVIPRTFEEVHEGAVLPGYVALIRPDAVYVRFLARLQGRAGLPQLSDVFVSDPAQIYTEGQSVRCQVATCDPDRQRFTLLLKPSVTASTDAAYLLGYFREMQQLQTIRAEAEGAVADGGGDVHDADADISNFFPLGGEVTARVHEVKEYGILCDIDDHPDVVGLVPSSHVGELGSSPALGSQLRGRVLDVVRHQGLVEISLKPDMLEAARGKAAAKNALKTLKHGDSVEAVVEGMRPGEYLILSLPQHASLLAYAAVTDYNTPRPDLVPRTFSIGQRVIATVAVLPSEAPGGRLLCHVPLTHVATSLIAGGAKVPLTPGSVVQTVVTGIQQDHLEVQIRDSKVSGRVHITELVDINDVTAAAVAAAAATVEAPPAAKKARKAEPTAAATGVNPLDSFRMQQEVEAVVLGRLYEQRDHGQRWRLDLSLRPSRLEAAKKGESQARAVKLTSLSPGMKLPCFVVEVAEDAVWLTAGPTLRGRVSIYEASTDPNVLAKLPTAFPIGTPVTARILAVDCKRKKLDLSLVDPEGSTTSGADTTDVVSRTGALVMGRIYAITGFGVRVKLGARGQVGCVALTDIHDHWVPNALKGLKAGTYVRVRVLGQKDEFAMLSLRPSQGGAVAGGRGRGSASAGKTSRGAVAPELLEESLLKVGNSVTGYVKNSGSRGLFITLDRFRDGLVRLSNVSDGEIKKLPAAFPRGMRLEARVIKVDGNGRVELSLRAPVRKLPSSSEPVIPSIKNLRPGQLVTGVVRSVRDYGVIVALDDKGFFVGLAHKTELAEQPMKDPSIAFTEHQVVRAVVIQVDAGKKRVSLSLKPSKIKSAQAAEEAAEKEARGKGAKRRRTADIDEEIAAAGGEEDDEEGGGEDQGQEGDGADGGDVDGEGEEEDDGDEADAAAAALDSDQDVDDVDVSEDVEEDGGEEAGGFDDDSEGTGVDDDDDDDDDDDEEEEEEEGRALQRFHPAGTASTVNLDLTAPWGEMLLADDPRVSGGAGATDDVAVDGSGAERKLSKGQKKRLKEQREQEIRAAELARLSGPAAPTTPADFERLVLASPNSSFVWIKYMAMHLNLGDVDSARKVAQRALDTINYREENEKFNVWVAWLNLENAYGITPSPEEAAMGMLKKALQYTDQKKMYMAALGIFERTGRDELAEQVERALTKKFGGSSKVWARAIERALQKNDGESARNLLERALQSLPRRKHIKALVRAALAEFRVGSAERGRGILEGVLRNYPKRLDLWNVYIDQELKTGEQQRIRALFERATHLPLPPKKMKFLFRRYLEYEKSEGDAAAVEHVKRLAMDFVEKSLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI61</accession>
    <name>A0ABQ5SI61_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051412">
        <property type="entry name" value="Formin_Homology_Diaphanous_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691:SF6">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="733"/>
            <end position="2278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="740"/>
            <end position="2191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2192"/>
            <end position="2210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2211"/>
            <end position="2270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2479" mass="249116" checksum="8F9872FEBDCF9A5B" modified="2025-10-08" version="1">MVISASADKPQRCVNSTVCLVRGNLAAVVTDDGCGPPTAIPAAHQPVVSRSTCSQRFPRISRYGCYRCSNKQAATGQVCHVRRPQLPRLCTWLLCLAALLLPGPGFSSNMVAATSPPIAQHSPAMLSPDLQSPDSVIPDIRNAALPPVGISTPPSTLNPGEDYGDPTNPAPPGEDVSCSLELQSLEPGSDVEVAVPTIGSGIFPSGLSFPASYAGEDCGCRSPPYSLVFGAEAELRRRKLHTFRLVPPPSTRFASRMASSSRGPVSSLVIQLDANRGTKCYYGDSGVGGKPKAKLWFSPTVPWEGDDPFGLPVQFSSSLSNATSPNTVELALNLTKLREQLETALGGHSAPPPGRENPGLGPGVGGGFEEDPLTLGVFLMTRCREQNVQRLCNGPKERHMNNTCLVAYVWSERMAMPPKPLSPGLPSPPPPPSPQPPSPEPPFLPPPPLPSPSPPPPSPPPPSPSPPPPSPPPPSPPPASPSSPKPPSSPPPSSWPPSPRPPPRRRLPPRRSPPRPRPRPPSPRPVSYPSPVKQPPFLHAPPPPPPSPPPPSPGPPSPPPPSPVPPSPPPPSPRPPSPPPPSPEPPIPAPSPRRPAPALPPSPRPSPSPSPSYPQAPLPDILQPERTPSREALPPPPLPLPASPRPPPRSFWRLPPPPPPPRRSPHKMSILTLKIYRQLAEWSSRDIADWHAAQGSSMPGRIQSTQEARALMQTALETGMYARDLAVCYSRLEPDTSFFPSPPPINMPPTPPPPSPVPPLPPPPSPPPPSPPPPSPASPSPELPSPPPPSPPPPSPPPPSPLPPSPTPPSPVPPTPPPPSPPPPSPPPPSPPPPSPLPPSPTPPSPVPPTPPPPSPPPPSPPPPSPPPPSPLPPSPAPPSPVPPTPPAPSPPPPSPPPPSPAPPAPVPLTPPPPSPTPPTPPPPTQSPPSIDPPSPVQPSPPPPSPAPPPPPPSPPPPSPPPPSPPPFSPPPPSPEPPSPAPPSPPRPSPPLPSPPPPSPPPPSPWPPSPLPPSPPPPSPPPPNPEPPAPVPQPPSPPSLPPPSPPPPSPVPPSPVPPTSPRPSPPPPPSSPPPSPLPPSPLPPSPPPPPSSPPPSPLPPSPPPPSPPSPPPPPSPAPPSPVPPSPPPPSPSPSPPPPSPPPPSPPPPSPPPPSPAPPSPVPPSPTPPLPPTPSPAPPSPAPPSPVPPSPPPPSPPSPLPPPLSPSPPSAPLPKSPPPPSSAPPSPLPPSPSPPSPPPPAPLPPSPPPPSPPPLPLPPSPPPPSPLPPSPFPPSPPPPLPPPPSPPPPSPAPPSPDPPSPPPPSPSPSPPPSPPPPSPPPPSPEPPSPVPPNPPPPSPPPPSPPPPSPAPPSEPPLPPPPSPSPPSPPPPSPAPPSPVPPSPSPPSPSPSPPPPSSPPPSPPPPSPPPPSPAPPSPVPPSPPPPSPPPPSPPPPSPPPPAPPPPAPPSPAPPLPAPPPPAPRPPAPPPPSPPPLPPPPSPPPPPSPSPPSPPPPSPAPPNPEPPSPSPPSPPPPSPAPPSPVPPSPPPPSPSPSPPPPSPLPPSPPPPSPPPPSPLPLPPPPSPPPPSPLPPSPAPPSPVPPSPPPPSPPPLSPPPPSPPPPSPEPPSPVPPSPPPPSPPPPSPPPPAPPPPAPPPPAPPPPSPPPLPPPPSPPPPSPAPPSPLPPSPPPPSPSPSPPPPSPPPPSPPPPSPPPPSPPPPSPVPPNPPSPSPPPPSPPPPSPAPPSPPPLPPPPSPPPPSPAPPSPLPTSPPPPSPSPSPPPASPPPPSPPPPSPPPPSPAPPSPVPPSPPPPSPSPSPPPPSPPPPSPPPPSPPPPSPAPPSPVPPSPPPPLPPPPSPPPLPPPPSPLPPPPSPPPPSPPPPSPIPPSPPPPSPPPPAPLPPSPPPPSPPPPSPPPPSPIPPSPPPPSPPPPSPLPPSPPPPAPLPPSPPPPSPPPPSPVPPAPLPPSPSPPPPPPPAPPPPAPLPLLPPPSPPPASPLPPSPAPPSPVPPSPPPPSPPPPAPPPPSPPPPNPAPPAPLPPSPSPPSPPPPAPLPLPPPPSPPPPSPAPPSPLPPSPSPPSPPPPSPPPPSPPPPSPPPPSSPPPSPPPPSPHPLFPSPPNPLPPSPLPPSPTPSSAPLPNPPPPYPSPPSVSPPPSPPPPSPVPPPPLRPSPRPPPSPPPPSPSPPSPPPPPRPLPPPPIPYISPPPSPLLQLSPSTFPLSSPLSPSLSDPPPPPPPPPPPPPLRPPPPPPEPPSWPPQPPSPPQPPSPIPPENPPVAPPRPPDPPAAPDYSGGVGVLCRPGKQGAVFADGEQFPYGQFRSSVCSCDSSPFGFMVGPEVMFRNVSYYTFRLYPKPCNTSIIAEGVSVCNVTGTNINKIAIRVDYAALLCFNQSRMTTIDPGYDYRGVRFWPDSVNATGTPPWVQARVGKSFDDRHIELFIYGLKSTYTDGFFMVTKCARVGSFSNYCKRSADGMCLFSFVDTPEHRFVSICRPLGMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ51</accession>
    <name>A0ABQ5SJ51_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014196</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="235"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="910"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1047"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1384"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1445"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="334"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="543"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="566"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="629"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="686"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="698"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="712"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="757"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="811"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="823"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="838"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="910"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="923"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1011"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1047"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1400"/>
            <end position="1420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1587" mass="162676" checksum="52DE9EB0D6224748" modified="2025-10-08" version="1">MSTARDPSVRGWPVYIGPQRNRLTLYLRSQPSIVFSSYVLRHKAGADLPRLSSANDQKPFSGVVACASQRRHGSGSLDDSHLYGGPHHGHYAFVSALFHGEQSARLAGAAQRLAWVPYGNALYLPLWMRGGHSEWWRLCRTQVLPPQPQPPLKQQKRPLSVLVQFPPVVAVDDEQTGAAAAASAAVAASELASREAGGGTTGVSTCYRRSLGPELRFDTGSVETSHGDVADAGVTASEREAQRPGASADGGFGGVGIGMSRNDVGLPPPPPPPPWEHGGTGGLPTPDAPVPRAPSGPEQLQSLPRGRGNPGDAPGANDREDRLKWGSVSQWVLAGGGREGGGGGGGGGEVASRMGAVVSAPDRMLGEAGRHDQQHERHRHEGQRQQKPLFMRGSDLPARPRQPPPLPQPTGLPSPTPQPARGPSPGFSPGGETEVPNPNPNPNPNPNPNLDPQQRQGLDLLVPAADRAVQPSPASLRHQNQSLEPPPSPQPLPRQGSPVSQGADGRPPGTCSPGSTGAGGIPAEGGFVVAKAGATRTAQSSSPPRPPLPPPVPPSPLAATAVAAAVPPPPPPPPQPPPPPPRGPLFVRGAVIPFRTPPAPQPAPTDAAVISGSPGPYCSLPEGLDTTDSPQQPSQQQLQQQLTGVRSAGGDRQAPLDGSRGSSCDIGTEGGGESTPDLRKTESAPGRSSGSSSGRSSGSDRGEEEKGKADGPPSPSALPSPFPQPPPHTPHPLPTPPLPRGRPLFLRGAAIPFHAPLSSNQTTMPQPTLGAGADHPLQPQPQPQEEEAEEPPDPERQQRRLPWSPMTQLQRRTDSVGKHCFVDTSNDCGSQEVSDGVDNPNSNSTPSGSGSSSSGRTSTDTNGPAQSAAASAAAAAAAADTESAGTRFPTAFASAWQSYEALLNARVQAGLQQGAHQGMQLSQPPPAPRPTDPRPGPVPLPEQSSLSRSPCWRPSAFSAEREGPPSSQPTTVNYRLVPLLPRENLDERRVMGGGQRPAEMAPIPPPPPPSRSATSSTSAASATLSTSSTSAASAASAIKYNTATSATMPLSQGYPQPQPSQRQPGMPPYASTTGRLLVPALYIPHDDAVVGEPPRSPHSPPMVAAAAAAIPTTAPPAAATTPATASDGDHMTITIPAARATAAAEVPNSVVAATAIVGTGAVATSPTVAAYRAGRSTSVPVGRAAAPGSHGEVAVRKAQGRLYEWELSVSCFGAGLDSRARLLSWARVSTLPGGDLRLPDAVLGAEQQLMRLQGEWTIPLATAQGRTEHDAAAVAAAALVAARSASGVDVTAVAAQAAMRRVSLKALAAASPSAAQRDRLLKLARRGRLKGLDPAVFQALCSDSVAATAATAVARADSFESVHIGSHASTKMVPPVSPGLISSSSSYSVAGEDGLEKHGGGGGGGGGGGGGGGGGGGAAGGGATVAVDEAAGGRITISAGLLLHHVLAVQELEEAEENAEEARAAAAAAAAGLPPPISRRQMGLVRSLLRRAARGVEADAGSWLRSVFLGAVPERRLRQLVAELVGEEYGGSSVEYGVTSYQYRASAVLPRAPEAALEELFGLLTRGSVGEVIERLKRQVNALPRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ70</accession>
    <name>A0ABQ5SJ70_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_014374</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="481"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="562"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="598"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="629"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="655"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="56"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="718" mass="75035" checksum="9B3F9BF9AA9C92F0" modified="2025-10-08" version="1">MLRPSVNSWFRGNGRMSIAHVNRDPYRARPPLRLWLVANSSSRRPHLRLTLRHTTTAPAAAAHTGSGLPYPGGGTGPHDGEPSDTDQSQLRRSTRIHEIVSNEGPTCSPSHPAAQGRGNISESPLVESRLQSILDIASFALPLATQNVLGYSLNAFSGAIIGRLGPTQLSASTLGNSTYSITGLSLVWGGAAGLETLCGQAHGAGNFRLMRLVLLRAILLSWAVCLPVTVLWLNAESLLVALGQSPSLVAEAASYLKSLVPSLFAYSLSEGLQVYLVVQGCTQVTTTAKAIATALGPLYYWGCMFGLNLGLVGAGYAYALCKATNAAVLLGWMLRRALRRGPPPSRTEVRGQEEATVIGENGGEGQEGLMTTVGRISQQQAATNGSASYGGSYVSDGDSALGGEAAKPDPNLEVLTVPLSAAAATCDFNQQQQQQLVTAAAATSRFAVSGLYRRLRILAELVHSVVAEALDLRACREYVRFAVPAAAMCCLEWWAYEALVIAAGWLPNADMAVGCLGICFTVSGMMYMLPQAIATATCARVSGALGSGDAAAAQRHYQAGCLLVGLMQSALCVCLLASAGRVAAFFCPDPAAAELTASLLPITAIGTLGDGMNALLNNGVLRACGRQALGARLLLLSYWCCGLPGAYFAGFHLGLGVAGFVAAIGITSILQSLVVGILVSRWDWHTEVRKSQAILTNMAPKVSSETGSSNGSHIPAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJI4</accession>
    <name>A0ABQ5SJI4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014479</name>
        <name evidence="3" type="ORF">VaNZ11_014507</name>
        <name evidence="4" type="ORF">VaNZ11_014509</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="3 6" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="2">
        <location>
            <begin position="152"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="522" mass="57398" checksum="D3E0782A3D3052C5" modified="2025-10-08" version="1">MGELRSRAPPPVVSNGGNGRSTSSAETANNQMGPNASATPSTSGATAGAGLSGSQPDTRNWSTTEGNSDDDEEEDMETDEGLDEDEGFVGRYPVRDRKRPCEWWRNERTVSQRVNIAIRLKEPATYEEAVRGPQSREWGKAMDAEIEAQLANGTWELGVPPKGAKVLPCRWVYKVKTNPDGSVDRFKARLVAKGFAQRKGVDYGELFAPTSRASSFRALLAVAAARGLQVHQLDVATVFLNGELKEEIWMKEPPGYESKTPGVACRLLRSIYELRQASRCWYDKLCTKFAEIGLFPSKSDPAMFVKVDEKGVILALVHVDDMCVAAKTQELVNRIKRAIGGLFKVRDLGEIKVFLGMEVTRRENGDITLSYVERILSAHGLGDVKPRVLPMAVGTRVTPAKEDAEVLEDSTAYRALVGELNYLAVSTRPDLAFALSVLSRYMAKPTKAAMGMAKGVLRYVAGTRHLGLVFRRRSSEVFDGYSDSDWAGDMDTRRSTTGYVFRVNGTAVSWSSQLQRTVAAHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK32</accession>
    <name>A0ABQ5SK32_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Coiled-coil domain-containing protein 104</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_014638</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CFAP36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1520.10">
        <property type="entry name" value="ADP-ribosylation factor-like 2-binding protein, domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023379">
        <property type="entry name" value="BART_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042541">
        <property type="entry name" value="BART_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038888">
        <property type="entry name" value="CFAP36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21532:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21532">
        <property type="entry name" value="PHOSPHODIESTERASE HL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11527">
        <property type="entry name" value="ARL2_Bind_BART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BART" evidence="11">
        <location>
            <begin position="4"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="144" mass="16236" checksum="1652E242082B492F" modified="2025-10-08" version="1">MGDQSELLQKLEDFFCSPKFTCAIGEFMGESAAKLAFVPFEEEQPLENYDIFKSYASLMERQLEDFIHSEGLTVKDVCDACTVAQNAESHTHLAAIDYLVASTDYESFMQLAYEHHIVSSYGPDLEEEEEAEEAVTEVEVEQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKF1</accession>
    <name>A0ABQ5SKF1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rad26/CSB-like winged helix DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_015106</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22254">
        <property type="entry name" value="CSB_WHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058951">
        <property type="entry name" value="WHD_Rad26_CSB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25875">
        <property type="entry name" value="WHD_Rad26_CSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad26/CSB-like winged helix DNA-binding" evidence="11">
        <location>
            <begin position="115"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="74"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI70259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="210" mass="21322" checksum="D63C1E167C3414AC" modified="2025-10-08" version="1" fragment="single">QWWRTWLVRWRRNGCCHQRWWGGAVAGTQALSHTHTGGRGAPSSSVLLAQIRSRQQAVHGAAVAASLGADLAASNARHGGSGSGSGSGSGSGSGSGSGSGSGSIPVVSRLTAAERLCEDLVSFLEERSGVVGSDELVEAFRSRVVPEQMPLFKQVLRQVAGLRRRAGGGEWVLKPEFFTASVVAAAASPGGCNTEGGGIGGGETGGGAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKR4</accession>
    <name>A0ABQ5SKR4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MoaB/Mog domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014851</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050101">
        <property type="entry name" value="CinA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056596">
        <property type="entry name" value="FLAD1_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE AMIDOHYDROLASE PNCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939:SF0">
        <property type="entry name" value="NMN AMIDOHYDROLASE-LIKE PROTEIN YFAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24102">
        <property type="entry name" value="FLAD1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="41"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="289" mass="31116" checksum="D78A2FC7975126D5" modified="2025-10-08" version="1">MSITGLGQIVNRRLLLGLCGERVPRRCIACATAAQDTPRAALVVIGDEILAGSVVDTNTPWLAKLLHSRGMDLIRVTYIHDSRSEVREAVLDSRARVGRNGFVFTSGGIGPTHDDVTYEAVAEAMGVSLQLHQPTVERMKLSYAARGMELNEARLRMATLPTPSEVLFTEGLWVPLVNVGGVYVLPGIPRLFQAMIRAHQERFTGVAFQSAALFTHVGESDLAQPLSAVAVKHPNVAIGSYPNTSADVSPSYKVKLAFSSRDNAALVAAVADVRASLGDIFLDQKSQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLB7</accession>
    <name>A0ABQ5SLB7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="181" mass="19619" checksum="749A74C4207287C7" modified="2025-10-08" version="1" fragment="single">RAAIPKGLKDERSRQLEYELNWCYNRNNWYFTGGGLLLGLTLGYTLRSAQPLAWAAILAPAGDWLYEQHACRDLQEAFNEHQRRLKRDAREAADRARAEVRDFYMQLMAEGTSPEIAAAATATAVADAAPHFSGSKASHLDGYGLEEAGTGGEGREEATAEVSQASLSRRAEADVGGTGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMP6</accession>
    <name>A0ABQ5SMP6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA topoisomerase VI subunit B transducer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015657</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000095">
        <property type="protein sequence ID" value="GLI70790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00823">
        <property type="entry name" value="TopoIIB_Trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015320">
        <property type="entry name" value="TopoVI_B_transducer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444:SF1">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09239">
        <property type="entry name" value="Topo-VIb_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA topoisomerase VI subunit B transducer" evidence="2">
        <location>
            <begin position="39"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="293" mass="32198" checksum="444075069E2C29B5" modified="2025-10-08" version="1" fragment="single">RLVGEMQAGVEGDMSPRQLTDKQVVRLHQLLHEVRFDDPKGDHLSPAGEYNLRLGVMKELAPDMIATHQGDVRVFEGHAFIVEAAVSVGGRDIKPGINVHRFANRIPLLFEGGSDVITKTALKRINWASYKINQATDKVGVFVSIVSTKIPFKGAGKEYIGDDVEEMVAAVKSAIQACCLQLKVKIVRAIAAREQKQRKRNLTKYIPDVAKAVYSVLERMAERAIEAGGGGRGSSGSALGDKEEVKPEQEATAKRRRLEVHDSRQLLPAVASHELTAATLQARLTEYVERIDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMU0</accession>
    <name>A0ABQ5SMU0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_016050</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221:SF193">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="121"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="359"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="230"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="309"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="415"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="609"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="437"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="466"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="479"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="630"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="644" mass="68571" checksum="87F85CD3D3DD74C8" modified="2025-10-08" version="1" fragment="single">LRQLITMDSSLPVPRPTSTRVAAGGAPGTTPGHLNPLQALAHHLVNPRGATHDSPVLLPLLQQVEASPTTGIHQLCPPLFQSQSQPARLPLPIPARTEAASGAEASRGIKREYEPRVGNGKQSVANSDGWQWRKYGEKLVKGSPNPRSYYKCSHPGCLAKKIVERSDADGTVLSTEYKGDHCHPAPLTCKASRFRPKPKSEQHAALSQGTLSLPSVGMLPMTGGGMLPIPDALKSDFPVSNQGPGSLAAEQEDETETSEAEPAPVLKSAPHDARPAQAAAIVIRKLRESQDSPSKRLDLLAAYAEEAERQLNSNSNSPEAGPCNKRQRTEAGGARARAHGDEDDEGTAAPSGSGMQRVVDMANMDDGFRWRKYGQKQVKGSPFPRAYYKCTHVGCTVRKHVERSAEDETRFVVTYEGTHNHRAPTGARRRSPKDVADAEDEEFEGEDAEDDSSQPTSPNYGPHGAQVSQVHQQTASKGQQPPQQQPSQQQPQQPGSAELGQQLHLQSSLLASSVLQQAALTGVLPLLPYNTSLSSEVLATLGLSADTLNGMEALNVLNVSNLADLNNLLRQHAQIDMALAAQAQAMDAACAGWDPLACLITPRPNVSPVGAQSGQAQAVPSAGTGKQGKQAVTQARQQVATTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMX4</accession>
    <name>A0ABQ5SMX4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glyoxal oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015818</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.80">
        <property type="entry name" value="Galactose oxidase/kelch, beta-propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037293">
        <property type="entry name" value="Gal_Oxidase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009880">
        <property type="entry name" value="Glyoxal_oxidase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208:SF21">
        <property type="entry name" value="LOW QUALITY PROTEIN: ALDEHYDE OXIDASE GLOX-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208">
        <property type="entry name" value="SECRETED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07250">
        <property type="entry name" value="Glyoxal_oxid_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxal oxidase N-terminal" evidence="2">
        <location>
            <begin position="264"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="497" mass="54058" checksum="A82E5687D744691B" modified="2025-10-08" version="1" fragment="single">MCQNSRIRSGAGRKTSPGRTQLTVGRWTGRQLPTVNADYGTLQSTASAAIAKSDTLHDDDGGSGDVMEGAFSIGESRDRRYLATAKHPPSSPSPVRVPPPPRRPQTPPRRPPSPLRSPPPPLLPSRPPRKPPSPRPPSKPPPSPRRRPPPPPMPTPSPSSPRPPSQPPHPLDPPALLPYTSFTDSGEAGPANGNPNTPMAGDDLVTLPTVPDNQPDVYGQWVLKAVGNIVAIHMSLIPGTDKFFFMERPSGRHPDRSNNIIGYYNYITNRFTNVNYTDSVFCAGHTVTQDGHVLVVGGHIAKTGYADGLKGVRIFSRKTLTLHRIANMTFPRWYPTATLLPSGKVTIMGGTVLPGSGTGKNPIYEIWDPANPTSLIQRSQSAGFVTKTNDIYYPNTYVLPTGDLFVFCNNYGEIVEPVTGAVRTTLPSWSNVAKGIFTEYPFTGTSVMLPLTPQNNYTPEVVYFGGQFSYGWINTTASRLALRIKVMYDPAAQNYTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN31</accession>
    <name>A0ABQ5SN31_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015681</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000096">
        <property type="protein sequence ID" value="GLI70804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444:SF1">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="120" mass="13996" checksum="45DC43D8B1F36DB9" modified="2025-10-08" version="1" fragment="single">VHLEEQLPNPDQWHGSVLSLTIEGNWQYYRSKIVRYLRQIAVITPYAHFCFTYKAEEDKNSMRINFRRRTDVMPPPPRATKHHPSSVDLELVKRLIAATGTKTTHCLPAQRVRLRQPCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN89</accession>
    <name>A0ABQ5SN89_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_015883</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF289">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE VIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="46"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="300"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="589"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="323"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="589"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="616"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="625" mass="64222" checksum="8EE99E818C35DC9B" modified="2025-10-08" version="1" fragment="single">MERSKELSFSVRNSGDAAQPAGMVGSTPGFVGDLQLLAGEELEARLQIHERIGGGSSARVYRGTLDGTTSVAVKILHPHAAQRESTLQEFLREAQVLARLQNKYIVRLHALSHLPPNYAGLPTRRFTWGIITEYMEAGPLTKLVEEAAKVYKQQQQIKQQLAIAATQSVGGASSIAPERSQSLRNLRPNLSFLRRRSLTSAADTAAPSSPRNRGGIAPVASGAVPASPSAAAVATGGGGGNSAPRLDSITHEMPSLPYTDREALQWCMEVALALQFLHAQTPPIVHRDVKTDNVLMSPLGPPQLSNPLLQGVRQNAEGPGLRSTATETGASAAGGPATASGTSFTAAAGGSAVFSSHGSLARRVLSIRAAAALADGLPSRQGSLKGFTVRPGGGRPLTAKLCDFGLHVVLGLGRPVVTVRRSSYEHTYGADMAGGGGAGSGIPYFNSLTRRSTRHSPAGLPTGGPGILSPSASIGAVAGGAMGPPSPLASVHAGVGIVHTQSGHLTGHGLGMGIGGRYEARSRRSSMICNRPMDENGLFRRAVSRDGDSFSRTQAGVHRKGSNVSYADSLEHAVPPAIPSSGRVLASITSGSGSSTMRGSSLSTAAAGSGGAVTGMFSSSPPKPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNH4</accession>
    <name>A0ABQ5SNH4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016011</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056922">
        <property type="entry name" value="SWAP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12323:SF0">
        <property type="entry name" value="CALCIUM HOMEOSTASIS ENDOPLASMIC RETICULUM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12323">
        <property type="entry name" value="SR-RELATED CTD ASSOCIATED FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25123">
        <property type="entry name" value="SWAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SURP motif" evidence="2">
        <location>
            <begin position="34"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="CID" evidence="3">
        <location>
            <begin position="235"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="695"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="503"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="542"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="784" mass="81234" checksum="5775A38BC611AC60" modified="2025-10-08" version="1">MAGNNVILQPGFFSPWEQMPAFAIPPMDATLGDRIKLLVKFAMSNGPSFIDMMRQKQAGDPKFGFLTNGEGSDYFRWLLYATAYQLPVDRAPPAGTYPPHATSTPAPAIPPAAPPGAPPQYHPPPAPPIPQGHLSHPAHPPPPPFPGGYFPGPPHPGAPPPPYGAGPPLAMHHPPPHGYPPNPHSQLPPPAHAATVPAPPPAPSMAAPQAPEPPPPMSGGAKASAPTAAHSTFGLPGDIAMEFASILDSLTSSKESIKSAASWFLSHQLHATGLALVAANRAVGYTDSEKQLHLIYLANDVLFKGLAQRISMGNSSDAISNAFQPVIGAMLAAAYNMAGRSDKIQERLNKILKFWSEKHVYGVDAMTMIEDAAFNTDDPAALLQAAHPEPSKSQPLAAPASASAPWQQLPQPQPPVQPAAPWMQAGGQVSAAPPPSQPPGQASGGWGPPPGAPTTQTGGPWGPPPAAPPYGHQAPPPPYGTQLGAPPPQQQAAWFAGSGAPGGLPPQPPHLHPPPQTTPGLAGPQPPLYPQAAAPMQPPAMPGMGLPPAAPPTAVTPSAGDPPMTSLQAKAAHIAAELAAAAAAAAAAAAATVGVVPLGNTKPVAPLPGPDLAGIPVSSTNFPPGLIPDLVKEKNRCEVPYTPLDPADIENAGLPPPPEKDSYLLARLERFYADLKDYRPGLSRADLDEAIRGGRIRSRSEEPEDVDGSRRRGRGAVGVLNDGSFAGGRNMSKYAGLGAHNAVDDISENSGPVEDDMYSEYRKQRSGSYHEMIFKSSAAAGAWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPC3</accession>
    <name>A0ABQ5SPC3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_017074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032727">
        <property type="entry name" value="CLAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28457:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28457">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14769">
        <property type="entry name" value="CLAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="269"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="275"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="35539" checksum="4EBF7DFE1ED90909" modified="2025-10-08" version="1">MARNLLWKDLTSQQLESIRHPHPKKGLTGRDLLAKYNGLTDYQQNRKTAIHLDVYINALQFGEEQKLSDDKLSGLFSIVKTVHHRSIDEALTMERSFLLFKELLLMHSVQRPPYSLGLFTYAEMRKIMEWMLDSYYRHYRLYQYSYTNRITLSATYSSPSDIVEIPPELPPLVKALTQAEHDAMLTAEERKREQEAAAAAAAAAEAAEAERQAKLREEYEAAIPDEIKDRVAAALEQELARLQRVMEEQFAAQQADLLAKIAALESGGGPGGKSGLTPGSSLITPGASVQRPMSSSSQSQAKHAAATAAVAAAAAASTAAPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ91</accession>
    <name>A0ABQ5SQ91_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000117">
        <property type="protein sequence ID" value="GLI71690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="770"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="922"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1244"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1245"/>
            <end position="1261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1453" mass="149388" checksum="6DEFB93A10EC06B6" modified="2025-10-08" version="1">MMDSPLVEDFSDAAAFVQEDTSYVEQNASEDEVERKIAEEHAKFKKALAKRQEEVRASRPWSAPPAESPRPEQQKERLKERQARGSIDDETDLDAPRAEYEPPVRPYLLQVQASGNNLSYMARPQSAEHRTAVRALNGHQSRVSLSGIEEEAGEGTACDSRQASEMGRVGSTFGEGMSTSGGSPDEIPDRDQSGIAKEDTLLEEAEGRRPAQEGTISEEDEEPAARRREGEEQGQEQGHDSDVIESGLRVKEPSAEEVVEDEESCGGRVGYASGDMEEGHRRLGNSGEIADPGGAGDGAVDAEVVDEGVLLASGEGLGSSALGVNHHDGYIQPPPASSPKGVGSLGALCPDAGRDHGDDDRGMEDARDQEGEEEQHQHEDEVGEEELGQLQDKGALVEVAILTARGAPSVASSSHDMAMRGFVEPLPYDTSYGGSVGRRSPAVGCKASEPRMEDEDEDYGEDTFEDDEEPEEREDLDVEPEVLVSEEAPSAEPQLSVSGERSLLRASPSASKALLSSSMRSRVASTVLAPLSKEPSRAFSITAGPPPAASASTASLPLPIFSAAVRASSTEVVDEDSSATTPPHALPAAVPPAQLSLPPVTSSSLPPIFKTSSVSTVSGIAAAEPNAIADQLSARSPLAPSHSQSSIISSGSVIPAPSVLMPPPAPAAALSELQPSQQFALQLLKHDIEVLPQHHRAAAEAAAARRRSATARPAGAGLAAFGYLGYHMVSEDLTAPSGPAFRSVSPGRGVSPGRGDGNLISNSSLKAVLSSGPLDTATSARSGGRPTSAPHMRPSTGIVADATAAAGNSSSRGRGTGAGDGGVVEPMATGGEEVIAASGSAFLGLAREMRAGRLLPPMMPLTAPRMGRSTSGGGAMATGGDGAPMPVENSVSVIGESELNAAVAQNRIKFDVWLDALSNGSPPVSNPRAGAPGPVSNLSSSGGEPDRAARRGGGGRRPPRPMTAPASGRRRPTSARAPGGAAPFMMPQATITIPKPLTRPMSAQPHDLRPISASLNRGGGGTTSAAAASVTGLFDVSIEAPVSVTVQPPDASAAGGTAAVEVGGVTVSVYVADTLKRIVEANRWLQQLGVTTKRYRLKDRFSNMTIQLVEELPPLPSERENGGPNDGGAVAIIKELTLKQFLSGYAKLKQQAKKHRNTAAAAAASALAAQQQQHSSFMAPGLSPGGPGGGGGMYGVQDYGTGRASRPISASFSSVGGAAGLAGPNSARVRPASAMPYSGARTSIGSGGGNDPSLYGAGGSGAALPGAQRGRQVRPSSANPAYGRTPTFIPYYTGSSPPGVPSSPFMHSAYAANATAAAGVAPVAKSPGGAGAGSLPQPRAAWAGGGGGGSAYPPYQGYPVTSARTSGGNAGNGLPYMHRSISATARTPLAVGLDADHPHAHLNDDNESERGMVIEPDERGDVSRAVRQQLGSASDYCQARRQEIARLRTLPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQL1</accession>
    <name>A0ABQ5SQL1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF444">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="269" mass="31181" checksum="619F4227DA5553DF" modified="2025-10-08" version="1" fragment="single">MKRQVGDLLAKGMIRPSTSPYSAPILFVGKKDGTLRMCIDYQGLNATTIKNRYPLPRVDDLLDKLKGSAYFSSIDLQQGYNQIRIAASDIPKTAFRTPFGRFKYTVLSFGHYITLVITNAPATFQAVMDCMFRPYIDRFVVCYLDDILIYSKTREEHLKHLELVLEVMKREQLFAKHAKCFWAQPQVEYLGHIVSATGIRMDPRKVAVVREWPVLYLSFAFAHKYLHVWRLSTPMREHLQVQQGKREQTGTAEDFKTKRDITYSLTDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQM1</accession>
    <name>A0ABQ5SQM1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_016959</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kinesin light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF3">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="737" mass="78674" checksum="D4C852A71E4C535E" modified="2025-10-08" version="1">MEEALESSSAPCQLPVLDTTYEQYGGRDPEAKELMQWFQKPGSRVAAMVAAGGVGKTCLAVDVAWRLWRCKHVSAGALYVDCRGAFSRDTLVMRLRAAAGLSGEHGGMEALLTSLAKLCAADRLGGSLLVLIDNAEDPAAADGGTTLSEVLAQLLMVSANIQVLVTARVDIAVQNMPQPYTYRIQPLSPTVAAALVEQLAQGVLTGQEARIVAEACGCVPLAVRLVSEALVAGRLTLEDVHDATAGRSGTVVTNDTGDTASTERKQEQDMTVLFVEMVLRSLPAAIKKVAMQVCAFTGVLTASSAAVVMGVGGSSEHEVRAQLASLHRLSVVLQHGDDSFMMHSHVRDVARKLLAEEAPPALRTAVRERFARYAASLLSRWQQLHEAKALKVMTAEVAAHWSDVEQFLADVAGAAAAAAAAAGNRPLLSDCGPLSLSPQLLRDTALLATLGNSDFLYAIGNAWMSPQYLMLTQKLVDVTAAAGVGAAAAAAVTPDRDQQLSYAAALLIRCEALIYTGEYEESESLAAQAVELYEQLRGRDSVECAGALHLRASCLQGQGRGEEALPLYREALAVRQEALGATHRHTTATITHLAMQLSYEGQHAEAEGLYRQVLEAQQRNLGASHNLTAQAVSNLAGCLHELGKLVEAEALYRRALDIYHDTVGPRHRQVAFTLVGLAAVVREGGDLEAAEELYRRALDVCRAALGPRHPHAQMTLGVLKDLLRERGKEEEAESLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RL79</accession>
    <name>A0ABQ5RL79_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="470"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="711" mass="75333" checksum="9092FAA5BF56F07B" modified="2025-10-08" version="1">MATGTHISAAALGETFKAEVFSKEVEGWLGRATPADRARFEQVFESIRNISEAKKAPNAHAGFVQTTLNKIKSGSARVASGPNASTPPLARGSPAVVCNGWSYTATRSAFDRPDTRNNILSIQEQVAALQEQQRQQQQQDIDAEADRPQSAPGTGAAGAGSTGRDRELGSFQQAAERRRALSFGATTAGARGAGGSSADAPVIAVAATIKAPDAATATGSGSGFVSLNPHVTRYQETFNLRSAYPEVYDQALRDTKVEQPPNSTFISEWGNSLKSGADEVQKLYMRTTYNTLNDEVVKFKTDAEAVREREFFKWMQKQKSFYGDLLSKDKIKDLDEVLANATDEQKHEILQALRTTHAVADDCFDRTRSHSQAVHCPMRRMEDETVEAMRRSYNKVRNTGQPLAMAPVARRAATAAARRPATAELKPTTVNLTIGGPGDDGGGSTASVAGSRVGSHASSTKALKPSRRPATAGAALGTGIAVSSVGGGSGVRPVSVPPPQRAECDKMDTFRSNVPLTWPTGSTGPEISSYEETYGKVGAGHVRPSSALFRVRPMKYNDTSCPYGAVNPHTAAAPSRTAYPVPEGYTASGAIAFPLPRGNTTYRNEFAPRDPADLALQLRAAAALAENGRKLLDRGSIPMGSRTGINILPSSMWVSEARESYVPHDVDPRALAQAAQAIRAMHMAPTGASGKMTLGGAPLPAAAGAGVARKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLL4</accession>
    <name>A0ABQ5RLL4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MMS19 nucleotide excision repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="20275" checksum="81351D53795B8BF3" modified="2025-10-08" version="1" fragment="single">LLNQGMLDLSVRGMAAEVLSSAASSSPKVADTIRLQGLATVTDLLASYLHEWLVQGVEDPCSESAEMESSVPTPQLPPCNTEILEPLLRLLATVTTSSTGEVPLSDQGLKLLGVAVRAGLAHTAGEGYKVATAAAQVALHLSCKTDVPLSPALCGDDVTEPMIEMVDLACRRAAAGDVKDARILHGGLCAAGFLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM14</accession>
    <name>A0ABQ5RM14_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24021" checksum="CC23E854938033D2" modified="2025-10-08" version="1">MPGRGQRHDLSGVPVIVVENVQDYRECIPHYVTCDDIVLEAGAAQGCTTSVIFNYAAEVIGIDKSPFNTRVAQKRYPDIEFLEIDAADISALLRLNKRFTKIFLDISGSQPVGPLAQLLEKYERAFPSVNLFVVKSYRMKKLVGCSTVFPEQVLGTTRNQTTEFMLQNSASKTLPAPTGSKVRRYRRHGATIGPAWLLALTFGAGAAVGTMWRAHRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMZ2</accession>
    <name>A0ABQ5RMZ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="52957" checksum="56978AECF5936986" modified="2025-10-08" version="1" fragment="single">MAAPAVASAATDAVRPSTSGNANAKKCLEDFFDRLEATVKKRQRIKLGGAQSIDWTKLSTALGAAVSKQKPPILPSSRTWRTDLAVEMKPEIKQHLREVFIRYCGWGDKQNFMFISRAQFIRFVRDMGMCNEEIDALGLSGLFDKILLGSLDPGAASRLALSDFLLAVTGLGTKLCPAVTVQESFDSVVSRYIVPRLNQPLPEPEPIDSALLHMDIVTTLERLRPKLNVLWDIYQQQQQQQSGAGHDSPPTSQSGLLFQADKAEMTISTFLRFTSDFEVTTVMLSRLQLIHAFKRSKFGVPTDPIDSNIHTKIRLNFQEFLDCVARCAMLAFDYKPPPVIVPTMDFKDSYRTAAKQMWLRQASASGGRLGIDDDESELEMPAAVADALGTEDRPQIDEGQTLSHAPSIRITNSAGAGGTSGGHDRLISRAVSIAQGRTQAPENLEMMLVHAEPTTMPADQNKPLHLQQFSCDWVGRVGLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPF0</accession>
    <name>A0ABQ5RPF0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pseudouridine synthase I TruA alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001288</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF9">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="5">
        <location>
            <begin position="524"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="255"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="318"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="403"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="699"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="406"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="699"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="747"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="775" mass="81949" checksum="E635F20A9ABB7C82" modified="2025-10-08" version="1">MNMVAVDILTEKPSDNEPGGKHLNVDQVKIVAKMPVQYSEHGADAAAAEEATEAAAPADSAHSKRPKDRVKRSVAIHTGYVGTGYKGSSINRTLGDDVTIEQVLEKALFAAGCISEANFGSFSKVKWSRASRTDKGVHSLATVMGLRMLVDDSRYAVGPEGDVEGLKLADSINQHLPPQIRVFTVQKTNKKFSARHRCEERVYQYYLPASMIDLKCDGSEEDAARLAAFRDALACFQGCHPFHNYTRRQVYQPGGANVQYRPGRGASKRIRDSDSQAFGQEPVPGGDTAGVTTQPPDAAGAGGDVTFCPAAAATASTEAAEAPPAAVGAGEGADGTDAEMHGAASEHAHQMRNQQERHLGEGDDGAGGEPDAKRAKTDDAAVVAVTPAAEVGVAAEAEGLAEPAGVGTGTVSGRHGGGGERGAAVTAGRGSRGRGKRRRRGDGAGGSDDDEDDEEEEEEDGGAGELDDDDDEEGGAGAAAATDGGVDCRAGGSDEPRRGRYGPDGRVVVVQWQTEIDPRDRVMQTYYRRIHHFTASDPKPLLEGGTSCLLLEVRGQSFMLHHIRHMIGGAVAVALGLMSKDMLMGSMQPPARVTVPRAPPHTLLLADCAFGLFPQQFGVGDNDLRNVTGERLSVRAGGLARREEFRERVLLPALQHLVDLPEWNQWKASLYDIYPFPQAAAELFVSSYLSFKDHKDAVKRARQEQQARRAQEGGQEEKPEGGEDGKDKGAGGKAADGWSGSTRGGDRSGWGGRGGGGRGRGRGGWRVGGRGGGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPV0</accession>
    <name>A0ABQ5RPV0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601">
        <property type="entry name" value="THIOREDOXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601:SF32">
        <property type="entry name" value="THIOREDOXIN-LIKE 2-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="53"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="244" mass="26666" checksum="CB0EA76BE37573CB" modified="2025-10-08" version="1">MHSLHPSEKLNCTSVSRSTQRAPACPRLFQAPLDATRVIAGPSFDRAVRPASRPQGRQNPPVIICQAGRGSGDPWWLKDNPANMKDINSIQELVDELADAGDRLVIVEFYAQWCNACRALFPKICKMVGENPDLLFLKVNFDDNRDACRTLGVKVLPYFHMYRGAEGRVASFSCTISKLQVFKDALENFSSPFCSLEPTPGLAEFPAIIAHPELHPEGAAAAAPSSIEDEAELHPLANTPTVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ30</accession>
    <name>A0ABQ5RQ30_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UmuC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_001423</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052230">
        <property type="entry name" value="DNA_polymerase_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053848">
        <property type="entry name" value="IMS_HHH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873:SF1">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21999">
        <property type="entry name" value="IMS_HHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UmuC" evidence="9">
        <location>
            <begin position="684"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="UmuC" evidence="9">
        <location>
            <begin position="1090"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="405"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="542"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="870"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1021"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="422"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="873"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="941"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="955"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1024"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI59531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1186" mass="124424" checksum="E4192196176D841C" modified="2025-10-08" version="1" fragment="single">PYVSSRMGNPAAAPLPVRALPGVGYKTEKLLAEQLGVATAGDVRRYSQQLLADKLGDKSGALLWHLCRGQDPSAFRPTGPPRSITVEDSFKTCSSWQAATTVLRVLAPDLLQRLHDEYDETRRRPETLTLKWRQRGTGWSRSSASCAMPLPPGALAAPPSQTHVDLLVRSALQLLQKQIPEPFNLSLLNLGATNFKRDGWGGGASGGGGGGGGGGGSILDLFSRHSGRQVATAQKPGSTNLAFGSATAAKAATNAPVSCSDVLDEQPDEQLDEQLDEELDEQLDEELDEPDSEYCPDVWIVGDGVRDCLVAGGKEHHDVWQLREGAVSGGGACGGGSAAAISAAEARRAAEQSAFLRREYLVSDQAGGGSGVNAAPAILSKSRERLLREAAAAAGTGPCSFLSAGGARGSAIHRSRPPLLQPPATRQQPQQQQRWLQPEMAASSQQQGAERASKLVDTSDRTAVGAVHTIAVGVGVSPPAVSGATREPSLDPHVAKRARLDPQVLSGGVIGDTVLGQNPADRDAAVITYRVVDLYYEINKGVRPDAGEEDGDDGGEGEMERSYSSDGCNSSSDGGGIDFLPDGRDSYLQYASREVELGLPPRCGKSRDDLLLLPPIAASPVSDDPPWSAFGRGVPVTWDAPYSGHGGSGSGRIDRERCGGKMGLSAAGHRQDSRAKEQQQMRVFLHLDVDCFYCQVERLDDPSLVGVPLAVTQFNSGGFVSVSYEARAAGIRCGDGVGAGGRASIPYLKAMGAVSRAEAQRRCPGLVIRPMRTERYRRVAEQLHASLRRFAPDVLVEKTSYDDFYMDVTAACCGEGPTATRSWAGIETGQGPGARRLCADAARVRGSSLSGMAPGAGGASISFGRSCALPGRTSLQAPQEQSPEGAVTGGRREGLARSSAGERNPAEGPTSSGCGAAVVVVGGRGGGAGGSEAPTDPSRVGGLGLGLGLDSGGQGDDRPPGLHEMDSSEPCSGDGDGTALELCQNLDLDPDRGQDLGLLDRCALKDGGIQLNAPEGGVYEAHRQQPQQQRQNGQQAGRQQQHKQQQQLLGGQDQFGDINQQDAPPRVHVVGGGCWEVVEPWLRRGVGIAQQLRAALKEELSLTVSVGVGPTKLSARLAGPSHKPDGITVVPAARLREFMSGVRIKAVPTLARKTGDQIISDLGVERVGQLEAWSRSELVARFGPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ41</accession>
    <name>A0ABQ5RQ41_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001582</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="298"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="445" mass="46962" checksum="8EDACB003450B9E6" modified="2025-10-08" version="1" fragment="single">PPPPPPPPPPPPPPPPPPPPPPPPTPPTPPPPPPPPPPPPPPPSPPPPPAPPPPPPSPPPPPPPPPPPPPPPSPPPPPPPQAQTPERSPPPAIPIPFGPTPCHVCSRVSINAPDNLEGKPLFNFGTNISCSYAADQMISIIDADVTSAGGRIGGFSLTTCSTTYIPGKVFPFVEVCGDIFNSSKVNFSSTPQKLISVVVFNRTSVVYQCPSGQFKNYNFSASIAPSNNSLESTECAAAYANLQCSDRLQPNAPSPPQAPPPSRPPSTPSKPPSPSPPRPSPSPSPPNPRPPSITMTHNCQASTANVPYLLDKVEDTMGKDVFGNPAVGRCTTVYSQPCVNPNNTCCGMDMAKVELLINPPCKDAVRKITVNGKEIPYSWAFFEKFYSLKFTNLDDPKIVSTPVGTTICWYVKPGVCQSTSNFCYLGRCQATMFSSDNKCCPANFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQE0</accession>
    <name>A0ABQ5RQE0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00043">
        <property type="entry name" value="CYCLIN_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052145">
        <property type="entry name" value="Mediator/Homeobox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330">
        <property type="entry name" value="HOMEOBOX PROTEIN BARH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330:SF19">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="152"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="489" mass="53450" checksum="0B6ACC5D68749864" modified="2025-10-08" version="1">MAYCRFRSSLPLDTAGGEMTSWVSPSSTPPSACLSSTTAARASSTPTAAFHGPHHSPTSIFSGSHQAQLLHQRLVLPLSLQQYPGQQPHQVPVAAPSCSELQTQTQLLRLQRQCYSQEKAQTQTQDLHVQFLAGSIFIPRFDVAPCQASADVAHHAAQRQQQEQEQQQLQQKQLQQKQQQQQQQQQQKLLLLLHQARRRIPHVTMCSFFPGDTVLPLPLKRPREGDSLAGLHMRSCRGGCSALGSSSSVFSRRLLRHNSIDSDDAQSQNEDNSDSSFGFVIAPTVATTATAIPAITETATIINNAIHYGKAPSAMQSVWKPCQSQQGQGHQGPTVQVWAHESVVCPVPPAAPRPTFCAADGEEFILQATRALRLSPDTCGRLAVHIWRRVGGIVRLVLSIRHPQIDGDNPRVAASAYRIHATACLWVASKVEEQRRVVAEARELAALSQTSAEMLCAAELRLLAWVEWCPLRGYLPSPQQRSAPGCGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQT0</accession>
    <name>A0ABQ5RQT0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001944</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF135">
        <property type="entry name" value="CSC1_OSCA1-LIKE 7TM REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="480"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="655"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="697"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="749"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="824"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="869"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="936"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1098"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1206" mass="133544" checksum="A59F9D7D534FB6F1" modified="2025-10-08" version="1" fragment="single">MEETSPKRKLRQSHHRPDRVDVVAARRSLVDAAQEASREEDTALKAEGLHIGSSDEKVASYRGVGLGLYFKLLHWLFWMMVWMMLCSVPYIITINTSVFTDYAHRYDHNYGVKVYDSFELASFTFAAIVDDKKDNGTLQYMNTNIADAWKGPMRKGVFLTWISLADAGATAVLSCVVLVLFVLVRRWLEKVDYKTREVADYAVIVGGLPPYVTATEVGDYFERCFSKDPKRDKVMDVVLISGFSTVIRACKEAHRNEQLKRFVAGMEAKVSAAHELPPITEADIPAAPSSIAESMVEKAGTPVTPVPKQTTPKTTPSSPVPANGGPRTISLTAIATATTPVSPPPLSPKPVPTPAPPASVTSIHDPESGALKKCSSLTRDMRSEMAKVMAKIREQEDLLLSRLTGKRKLRSNAAIVTFNTEQMRAEVCKMMPSGWWASLFMRRDLRMSHGGRLWKVWVRRAAAPDDYRFENMATRIRTNILIQLFTGFIMFIFVVICAAFITWLSAASNKEARVIKWDMTELRAAIGIGMAKIGAASTSNTAPDPASYDAFCASSMASCIAHVGEVFPDAAINMTYGAEWSFPSATVRLLNERPIRQDLTKCAESVSCPASYCIPCYCLGLSSVPTDDATPEFLAHVKDTCSTYWRPLDLRRQGIMIAISVVIAVINSLLTTVLRVLKFNVEKHWTTTDTELSYAMWSYLVKLINSVAVLLIVNCSTLGNLQKRALEHESRWMQRLVLDGLFDDFTPNWYASIGFSVLILMMINVAGPLIRSFVDIAVKWVMRTRLLYVHMGKLAAPEDYNAAFRNRQFTLEERISDVLFNLTLALLFGSGMPLCYLIAAVYVLVAIWWDRLNLLAWRQPAQRYHTNLPLAIIYLLPVLLMGHCAFGLWMHTYFKAELSSMDIMAAANAELVNPGISSLSHSSLSRRITQPNGLPLLIWFLVLGALLIFGRWILWVVFKIFGGSCARLTTSMRSRLGSRDGNGHSDVSYEEALSTTKVAKLLRGPRTYRISHLTNYKAFLSTGVAARLWSLVQGDRRFTRLTTFRVHVEMEVHDGHQVRTEEREVEMFDVADVAIKSLPNAAAALAAANAAATRDDFPLTPQLPLPPSRGLAQPTGDAPRHYLSPPSSADTLHPLPPTDSALLGLVPSAALQAATLQHIATLQANTGALVKRVSLRAMPEGLLVPGNGSLKMVAPPEPLPPFELDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQX4</accession>
    <name>A0ABQ5RQX4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="212" mass="22732" checksum="C50BDC020BBE5FD5" modified="2025-10-08" version="1">MAGFLDTAANVLGEVADYLKTEAAGLQQVQGPQEEREGRQEMGHETPSEGPPAKTSTDTRAGADTTAVALDGSSDSLYGPEGMNATAADISRQEGEAELQGGRQQEAAREEGGGPGDTLRGAREGVSQEVQRLKERFVEGYRDARDRMAGEEGYQEESGRRRGGSEEGVGEEGGLQETLRAAKARIDEENEQLKRRFHEGYQRGQETGSGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSS3</accession>
    <name>A0ABQ5RSS3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05382">
        <property type="entry name" value="CAP_GAPR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034113">
        <property type="entry name" value="SCP_GAPR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="1">
        <location>
            <begin position="132"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="270" mass="28553" checksum="F887C7138D604DD7" modified="2025-10-08" version="1">MHLSSCCQPLLKRLCLATPSSYLNASACIVQPRRGDAAMHLQVIITLISLSSTTRNTSLGIRGRRGLCQVFRGYQSAGSSVSTHEVNIALAQLEAQATEDNASQAVKPTAGTGISQDATLGASAADQGAGIAGGADVLSLHNRYRAAHHAAALDWSPSLVSSAQNWANNLANGCAFYHSGMQGVGENLAAGYRDWAGVTDAWYQEVNQYSYANPGFSEATGHATQMLWIDTSEMGCATAQSPAGCWQRTVYVCHYNPPGNVIGEFPQNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RST5</accession>
    <name>A0ABQ5RST5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="8">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_002493</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="9">
        <location>
            <begin position="19"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="365" mass="40423" checksum="D25E9210B29D8385" modified="2025-10-08" version="1">MDSMQIASSSSDLVQRRTLLIDKHAAYIKSFSRLWETPDKLEFVATEHFWMSGMYWGLSAMYLMGRLEEMDEDAIVSWVLRCQHPCGGFGGSERNDPHMLYTLSAVQILALYDKLDELDADMVASYVAGLQQPDGSFAGDAWGEIDTRFTYCALLCLAILDRSSVINVPCALDFIARCKNFDGGFGCTPGNESHAGQVFTCIGALSLADGLHLVDRDLFCWWLCERQTKSGGLNGRPEKLQDVCYSWWCLSCLSILGRLHWIDREALTHFILDCQDEEDGGISDRPDDMADVYHTFFGIAGLSLMGYPGLAPIDPTWALPVEVVQRFKKRAMAAAAVVEDVGTDVTATMGFAKDQPAKASTSRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTJ5</accession>
    <name>A0ABQ5RTJ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF11">
        <property type="entry name" value="LIPASE YOR059C ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="1">
        <location>
            <begin position="6"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="457" mass="48451" checksum="7E3EEA3874820E73" modified="2025-10-08" version="1">MSTKSSQPNHLIVLVHGLADTLHAWDNCINELRKIEPSDFLLHASAVNGRFKTHDGFDQCGKRLASEIRELVHRQTHLAKLSVIGHSMGGMIARYALGILFNPVDGQVCGLKPIHFITLATPHLGLTVDDGPGQVPFVAWAGNIPLLGPSVKSALQGVGHWVARTVFRGTGRHFLALDGGPGELPLLMLLAMDAPDKDQYFYSALAAFASRACYGNVGRDHWVSWENATIRSTEDLPNIPPDIVRKGRGVIRADPPDVGLGPALLQTYSAVAAVDSAIGAHPLSAAANASGAITREGECSEILQRPVAASIAASSTRARGVPAAGTTGAYAKIEGAESGSEALQSILPSGIAAGQAAEAEALERAVEGAMESPAQVVEFVLERLRRLPWRRVDVSFAGTRLGSAHNNIQATRWTNRVGFSVLEHVRGHLTDVDRLLARSAAGATGREAPTDGSSEMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTY2</accession>
    <name>A0ABQ5RTY2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="276" mass="28786" checksum="AB8D6ADA1BD0ED12" modified="2025-10-08" version="1" fragment="single">VTLAAHFMDLELLFSMSPFDPTLRSMSDRNWGEVAGGRVGASGSGNGDRSGKLSILRPAWNITFDLKDLAKHHQSSSPPSVLAAGAAGINACGWQVLDVSGSGGGLAVGTTAKLLIQPHVLVKQQSSATGTVLQAAGSSSGSSGRGVGGAMLLEVFAAAPAVPISGCEGGGEAPCVGVRVTTPLQRSLAWCHCEMRVAVRKAQGVLVVTNRYSGIPVAGAYIKVFVMRDAQPSGEFYKDGYTDRRGLMDFWTQTLAPDSQKVRRSSALLACDVRHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU50</accession>
    <name>A0ABQ5RU50_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GHMP kinase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_003208</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00061">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004424">
        <property type="entry name" value="IspE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00154">
        <property type="entry name" value="ispE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527">
        <property type="entry name" value="4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527:SF2">
        <property type="entry name" value="4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010376">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="6">
        <location>
            <begin position="111"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="345" mass="37650" checksum="8D8AE8B4916A43B3" modified="2025-10-08" version="1">MLHRSGRQPSNVFPTRRPAHHQLANGARPAKRRSYVAVYAASRTNSIKLFSPSKINLFLRVVRRREDGYHDLASLFHVIDLGDEMEFSTSPYATSDTLSCSDPTIPSDDSNLVIKALNLYRRKTLQTMYFNVQLNKRVPHGAGLGGGSGNAATTLYAANRLLGSPASNEDLLEWSGEIGSDISVFFSNGAAYCTGRGEVVEDVATPVPLDTPMLLVKPPVGLSTPKIFKSLDLARRSTADPRGLLAEIATAKGVDQAAAVNDLEQPAFDNLPELAALKQRLIDTGNFRSVFMTGSGSTIVCYGSDSVPAFLKEEQYKDLFISPARLITRKEGGWYTHSGLWAEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVC6</accession>
    <name>A0ABQ5RVC6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dimethylargininase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003814</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033199">
        <property type="entry name" value="DDAH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737:SF9">
        <property type="entry name" value="DIMETHYLARGININASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737">
        <property type="entry name" value="DIMETHYLARGININE DIMETHYLAMINOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="17907" checksum="24A7B662A38D5260" modified="2025-10-08" version="1" fragment="single">LRSIVTNVVEIPADDSCPDCVFIEDTALVIGKSHAVVTRPGAPSRRPEPGPVAAAIHRLGIPQIDTLRAPATLDGGDVLVLPWAVLVGISRRTNNEAVSQLRAFLRDTNGPPLYVFSVPAAAATAAAADDNCDVTRAAASDAAHNNTLHFKSVLSALDTHTLLVADTPIGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVQ1</accession>
    <name>A0ABQ5RVQ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="137"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="430" mass="43705" checksum="63D330E8FE02EE10" modified="2025-10-08" version="1">MDRWIDADDDISARSRGPPQGRVARLGLAGFVQSQGSLGTENAKTFGKIEVVQSKRDQRKAEATRLMPPPRLPGGTATEAPTPVPEDVPSIPSLPAATGPPKCPVPDWAAEPTAGSRLLVYKDGQAIQEVPLVKIVTVFGRVDALADVVLDHPSISRQHATAAFHGARGTWMITDLGSTHGTFLGDRQLAKNDPTELVPGVELRFAASTRRYKLPPAPPGPQSRNAAVNAGGVAAAPGSESGRLPSATAAASAMEMPPPPPPKRPRVCFADDTDAGGHSGGGGEDGKPATLAAAGRPRQLENIIGFSDGRDFVARVGPRAAAPAEGRFAGAVATTIVLSPKGPSASAAAATAQRASSNGSGEAVASGGGGGGGGGGGNSPRPHSSVHGGAGAPATGEVRPQLRELVDRIRKETPRGGGGTLYEQLPPPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVU3</accession>
    <name>A0ABQ5RVU3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_004170</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="52"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI61750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="130" mass="14719" checksum="C46A832C2FD99337" modified="2025-10-08" version="1">MSDNEMEVDEQQEQEEQQAGPSKRGNKSGKRFEIKKWNAVAMWSWAICTDTCAICRNNLYEPSIEYQANPTGDPDHPGLSIAWGCCGHVFHLDCIQRWLKTRSACPLCNKEWEFAKIEKILPGGGMVGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWB7</accession>
    <name>A0ABQ5RWB7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003719</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="394" mass="41059" checksum="2DF9E04231702D83" modified="2025-10-08" version="1">MAAHYRRRDLPLHRKLWPAEGIPDGVSIKTHTHISVLHSIVCRAHASSGKEGSRGHAGSASQKDDPPLVQPSLVIPPPSTGSQYGRNRLLDLAFSGPLGVGGGGGDGEVGGGGFFGFGGRGGGAGGGGGRGPSGSRDDGSCRGRRFGVLPRVLVLIQFLCWSVVLYMLVDTVVNTSTRVSKKVWRTNSRVSGKEGRGQAAAAEPQGPQPLLHEPEPGQELEHGGEQEHREKGQKHDEQPEQGQRQEDKMTVDGEVVVVENEIEEVEEEEVVVTGDGAARDAIAEAMAGPSSSRSCSSTVLPPDGTEQPLATMAMRRTPDIPGVVAMGSVDGPAAAAAAVAAGTEALSGSPSPAHQAGQQRPPFVRYEGARPVEYPTLAVFIMILGSVGFFSTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWE9</accession>
    <name>A0ABQ5RWE9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gfo/Idh/MocA-like oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003720</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF5">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="3">
        <location>
            <begin position="155"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="41349" checksum="6EAC21E9CF3351D6" modified="2025-10-08" version="1">MTPLGAALLGTGIFAKDIYKTNFKEKENEVDLKAVWSRTIESAQAYVQEFFPRSRPVAGEEALQEIIEDDSIQLVVVVLPVQAALKIVKRCVAAGKAVVQEKPVAATVAEAVEAISAYRRAVADAQTPQRTPLWMFAENYRYEAVFVAASRRVSEIGRVIKLDLIADLPMDERNRYFGSVWRRDVSGCPGGFFMDSSVHFVAALRMLAAAAGLGGAVSACAHARQTKPDLSFPDSVLGFARFANGDVPASISISLAAHQVRWSLSVVGTEGTLEVSRGGWGGTRTGYTLSIKTIRPQEASQPGQTDSAVQVLAYAFSGCQDEFAEFVGLTAELRRGAASAECGGDAAEVAVPNPAARSSPEEGARDLALIEALLASSAAGGQPVQVVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX67</accession>
    <name>A0ABQ5RX67_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="26439" checksum="BBBEE577F710DFB0" modified="2025-10-08" version="1" fragment="single">MSAEHDDTPHTSLASQPDFVVAARAPDRGLETVGARATQEPLFLQTPAHLIVPHSAVNTNMDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTDKTPGHSIADATRAYVRDAKKYLQAVHGGDNDGIRCLFVGNTLEGSAKDWYRNWTNARGNYTFDELVAALLARFATEVQARDVEARKVLAAGSYHMRRDENVPAYQSRFEALVTPIADLTESERVFWFRRGLTKILAKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYB6</accession>
    <name>A0ABQ5RYB6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ANK_REP_REGION domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005273</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2079"/>
            <end position="2098"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2150"/>
            <end position="2170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2208"/>
            <end position="2240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="831"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="940"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1147"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1266"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1297"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1560"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1894"/>
            <end position="1919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1931"/>
            <end position="1952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2275"/>
            <end position="2295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="574"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="837"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="856"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="940"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1574"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1897"/>
            <end position="1909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2283"/>
            <end position="2295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2295" mass="236594" checksum="4B6685D961C8AF04" modified="2025-10-08" version="1">MLNVIVLPYPTTFQQALVVSMLVGIVTVLLHCVLRRRRLPDGRTLRHGIGKVQTQPAQMAKTHSEPHTCDDKAREKCSGHCSISEMRTRANSWATELFPQSQLNLGLEPQPAPLQNLCAYSPLGCHACSSPKSADSGIKTDRYHPDDDATPAAASASVTRSPSASFSVGPKPSGDLSQSLASSPSPAIVPMTPPGACPSKHAGGCGVTDVASVGASGATSGIGGALPSDTSFRPDNTSSAYDTARALVCHPWAQPYTPVFTLGRIALKIPDRDPSDLGALAVWTRPYCEALESEGIALVGVYVRQGCIHIVVDLLDLTASNSSGDNSGNGVSNGAGNGPYAGPRPRAPVDGRAATGGQHSPRHGRHLLGTEREEHPCSSSGSSRSNSRRRSGRVVAALTAGDPGVSDGSGVTAAAFSLANQSGGRVSAPGDLLPLAAPSRDRPTTQQHRPHVGRHNTPARVRSAEVAASGAAGPARIAGLVRVAAINAAATGGDRSRSDGDDDGGSQHEDNNGDDDEDDGSGTIAPRSRLHLLSARRLRQLRRASASSLLSGAAAVLGGGVPAPPPVPLPPLPSSTSHDSVTGSSAAAQPPQPGAATAFRLPATLTPETLVHLLGPDIGGGGQTVTVQLPSGGGTWSVQMRQAGGAAIVVPSPPSPRPPPDLTCISPPCVLAGAQGPVMMYAAGTDMHGVATGSGEGATAATGSDTPLTVHMRFRGAYQPCRASLLGDQMPAEALSRVLPLDALRRAEVLCLQLAVPPSLPGLMFVECQRGGLLGAASPVLVAPNAGVWQEIATLQDAAVREGGAALTAVQHLVIDLGLWLEYLTNLRTPPAPQPLPSQRQQQQQQSQARLQPLPSRLPPPPPAPAPLHVGTVQALPQPEVSPQQSTVYDDMEFSAVSDGAGGQPPTSSVAGSGAGIGLGRLFGRSLLRLLSDAELVDPQRQLTTSPQSQQLPQQQQQQQQQRQQIDGIRQLEEMLATAEQHQEKQLNPQRHAIMEQRQSWLRRDAVPGSASIAAAGTSGALGALEAVGLTRPAAGTGVAPETEQAAMGQHAGVGPQVLSPNMGVAFSSSPGPSADAQDPSAVCGDEVGVLDYMRAISDPQTRRVVMPAPSTSLARVENLGVQEARTGVLACEHMGVVPVPLALNTRSRPGGGGRAASGAEFEGRNDGDGGDEGAVLASVSIINPPAVSTRPLQPPSLPRAEAADALPSEVQGVLGMGLRTELMTTPAKPVVAASVATGSGGCGPCVNTCGLSIATVPQPPHAQSLFNGRWQLPSPPPSPLAGAVKATGATQLDQHICEDDGHMPPGTSPPTQAAGDCRGDSEPTDAADAGGLSPAVSINMLVLGPAEAATLSHALMGYGTSTRTGASGNLPPVRTESSGTELRSWDSLTEVAQAVSATLPLPSGALATTPRSCSTFIMSPSPSRRPSLHLYGDASRLRPSGAGSVLPGSCWPIVIGTAAIANTAGSRASSLDSLSERCRGSDDRESSPFLRCNLPSLTAEGVGETAAAEGSASSTLHYVAQDAEMGDAARGRAYHIAQMTAVRTEPASVDGVVCEGPQPALTPRPAAAESEIRPASPARAASPGAGEEIERTPAGTVETVAAATGAGGTIAGTAGSPDISSSDVSTAPAAGAACQAALAGAEAAVPLAAGAATTTASEPFGPPFAENAASLASASVLEASDAAWSQLTAGSSRPHGHWPAFNVTTIGEAPEITALLHARMRHLGLGLLIFSVRRGWLHITSELLGGLARVGMDLAEADAGVAAREGGMGLLHLAVTARQPALLRMLLAVATAPTAVGSGGTELSGAGRRKAFSWDVGARGPMGLTPLHLATALNDGGEVGTLLLEAFPEAPALWFTASDDSGCTPAGMASQAQLHGLTHWAGRRLAESAAWRWQHHPQQQLPNATDRPTQLVGTQQQQQQDVVLLQRNVQQRHEGGLQQPTQRARGGLSMQPQLQSDAFSFANQALSGGDSAVVGSGRGFGDDAGGLNSAAAAASTSAAATAAASSTALSDLTGSTARWNRGMRRRTAWSGRWLTWWNICSRPQQPPYRRESCLRASLTGFADSQMEERYRAYRVNSLSVIDTTCGLVFTCLFLLPALELAWQRRVAELASHCIFNAGLLGPYLLQAVAPALFRRHRDMVLCFGDVFKALLVLLAMLGVMMLPQVWADFARGHMDVVMLVIIKPLSEQIQVWMALMQRTAAVISDTYMYAVVIYGGAILPAAVRAVLQNTAALVLLLLLDVRARLRFLATVSASSVGSGTDDAVSCKMVNAHDSSNGAGLQRDVRNVSHAKKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ03</accession>
    <name>A0ABQ5RZ03_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004993</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092:SF2">
        <property type="entry name" value="HERCYNYLCYSTEINE SULFOXIDE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092">
        <property type="entry name" value="L-CYSTEINE DESULFHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="138" mass="14552" checksum="B829CFFEDBE866CE" modified="2025-10-08" version="1" fragment="single">VGPHTNWKALRVGAGGTTVGSFTAGAGDDHAGRDDVGSSGGRGRGAASSQLLVVGGGGDWEEGGVAGMLAVQLPPLRPEYGCTSEDAARVQRWLRYERNIEVPVVAALGSLWVRISAQIYNEKMDYEKLGDSILTLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ11</accession>
    <name>A0ABQ5RZ11_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Association with the SNF1 complex (ASC) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004878</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043554">
        <property type="entry name" value="KINB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46316">
        <property type="entry name" value="SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46316:SF2">
        <property type="entry name" value="SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="3">
        <location>
            <begin position="167"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="269" mass="30053" checksum="59FACC9FB54162B6" modified="2025-10-08" version="1">MGNTTSHKHEPTVGPDNYHYRHASPPISPGSPLTYSPQIPMEPISRAEDVTANRGAPEFYGVAGWPAQPKVVPVVIVWSHGGNHVEVEGSFDNWTTRQPLQKSGKDFTIIKLLPPGVYQYKFIVDGEWKYDPNQPAMFDEMRNVNNVIEVHEYVPENLEGVSGFEPPPSPPSSYNCPTPVADDYAKEPPVMPAHLQLTLLNVPPALDAQAVLPRPQHVILNHVYCQRGQNVQALVIGTTTRYKSKYITTVMYKPKARRRVLDNNLRSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ51</accession>
    <name>A0ABQ5RZ51_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">14-3-3 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FTT2</name>
        <name evidence="3" type="ORF">VaNZ11_005749</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 14-3-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="2">
        <location>
            <begin position="8"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="248" mass="28294" checksum="372474D8EB92B643" modified="2025-10-08" version="1">MAPESTHRELALYSAKIAEQSERYQDMVEEMKKVAVLATDQELSVEERNLLSVSYKNLVGSRRASWRILQSIEQSEQAKGNEKRVHMIKKYRAVVEGELDKICQEILDLLDKYLIPSAQSTEAAVFYLKMKADYHRYLSEYKVDVAKAAAAAKTLRAYEDAQLRADELPTTNPIRLGLALNFSVFYYEVMNQPGDACNLAKKAFDEAISDLDSLGEDSYKDSALIMQLLRDNLTLWTSEMQDEDKNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ79</accession>
    <name>A0ABQ5RZ79_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_005226</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SHOC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF54">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="539"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="942"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="942"/>
            <end position="971"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="987" mass="104423" checksum="69EE94117BAFA8C4" modified="2025-10-08" version="1">MQVKNALVPELDGLRGTVWPEDLLVSLFSQAQREYDAPNLRLVNKEWRRIFDYSLHSVRIDHASSECAHFLAKLHGLTELNLSTTMAPGLGGDDIPETLLAVASNLPRLRSLVLGLQFLLRAKENVVPVVTTATASTSSVEAAEVKADALRHVRVSGIPSTTAVVKGPCSRYTILPATAVGALTGLRRLSIGGSGSLGPCLGNPPMELPYGSLTLELPQELLGLRCLEHLELYGIPLVPPRGCSAHLSRLSLLSSLHLAGSASSLARSATHLTDLIALRRLVLHCTAPLGISEHGDLGLLERMVELELADEAGAVKAAAMRRQPPHALSHGPTGGSTMAGRNIVAMLSTNSLLFMGLQRLAVAGVTLTELSYRGFFLTLPPPYLRQWSSLESLDLSHNTIMLERGILETQVACLTHLAHLDLSNNRLDELPSKLCSALLGLSSLNVSRNPISRLPAALSYLSTLTCLMAAGSCLVGLPPEVTVLTRLRHLDVSGNRLESLPEGLIRLSRLEKLQVSCNRLRSIPGLTQSWYSQVQSSASSPAWSSSPTAAADTSPRSAPTCSPTTSTAPAITASTTSHVAAASISIPSTVRGAELRSRDVDVDVEGMQGPDEEGNMACSCPWAPAVSLATSATAAVANSTDAASSATAASASTATSSTTANAPLPFPALNHLDLSSNSLGVPPLAVICRMSRLTTLAFGRNYSQSCLPDPTVTTTTTTTTTRTTSTPRLLDVAVATALLQPMPAPSRWDLPSRLVDLDIRMTAGFMHRAELFWLNVGQLTALTRLNAQSNALQALPPSWTSLTRLLHLDLSLNVLTHLPPGLSALHHLRHLSVSSNHMTGFPAAAAAALSALTFLDMSVNLLEDLPEELGEGMTRLRVLDLHYNSLCLLPPSITRLVQLQKLDVSRNYGLKELPYGIAELPGLTCVLLSKTLVDRTRAAVGSGAGTAVGPSSSSSSSSDSSNSDGSSRGSSQVDLLAARGVRVQVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZV0</accession>
    <name>A0ABQ5RZV0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005788</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="108" mass="11651" checksum="6F59B6A83BDAA630" modified="2025-10-08" version="1" fragment="single">VTERTTVYWLCPALKAALADQLGIPISFKPHPQGIACSDYYTLPKAKGVYYRVTVAAQSEGHLALLDSIQNSGDFVFQSNMVCGSQIRVGLGEPIRGKIVPVAPSPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZX3</accession>
    <name>A0ABQ5RZX3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_005261</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057992">
        <property type="entry name" value="TPR_SYVN1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25563">
        <property type="entry name" value="TPR_SYVN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="350"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="453"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="611"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="683"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="698"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI62587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="721" mass="79060" checksum="99CA808ADD885B73" modified="2025-10-08" version="1">MPQQDQHHHQRSTWPQLFGIHFGTACVSTAWSLRNITSPRERTWGALVQDAEALLADELTVCVLLNFCCSVFGLIALCTSTLFLGRLSALETHRLQQRLFKCIMHKIVIVRVVIQPDVRDMAGWLAWLAVVAYLRGFVGAAKDRLESLTSMPGGQPGRHARGVCLLLLMMICNAAAFMVMRMMWASSIARILLCAFDTAVVAIEGLRIMLRYAVNMVDRYSNLGGVDPSAPPTRTAQDADLPGGTAAPTAAHADGGWDLGWSGKGSFQYHAELVADVLIHAVTLVHYGHVWALHGFRFHLMDFVLFLEMRGVMLSLVRRLNGHLSYRAATKHLDTTFRDVRPSDGTAIDCSICLDEIAHVAKQLPCGHVFHLSCLRAWLQQSGSENFTCPNCRQPIITDGGIATFPQPDWWIVRLWERLGMWLTLVVEPLLLMLLINITVWLGGRAQVRRRAQRAQPASVGEGFGETPGPSSRHISRGRSRTDDDDSSDLETEQKTTHEEAEEFDNFDARVTANSEGPDGMPRNAHRNNRRSTRRNNDLNPDDFDVDADIGGATAVRARRGLRGTGCGDEGGCCVAGPSASGPAAAMRSRRAGRGIVIRLEDLQEDQVVRHHGAGRRARQMTAGDDGGAETAPSAVEAARRPSALRRAQPPGELPSVSGEVLHQVSEMARRIRRTISVSIRSSWAAVASADSDGDEEVSSRRQEAHSRAADPQEGSAGGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S078</accession>
    <name>A0ABQ5S078_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="444" mass="45712" checksum="41F00B01753F9CDE" modified="2025-10-08" version="1" fragment="single">MLHARRQIIWIEDTTTLMPVQAAHNLAAIHYARDSRLKLQANGGKIPDGSDSDGGKDVKELSKWSTAHPMASVSSVLMQSVEPLEDFAAGVTELMPNPTAPSAAEVRLVLARRRMLDRERAQHAQTAAGNDTSSQAHQEQPRIPSGGGHGVVNQGMLTPNALVDKPRTKTVEFEDKPGKNLPQGTADTTGCQGVTESSPARDSSIAATAPPASELVQEASQDSRLVLPVLESLIALRRSRLTRPTQPAQQPPQSPHPPVRPSNMSINGGGGCGGTAAGDVATWYGASGTPSPGAAAKPTGAKTTRRFGANTVNTNANTNASGAAAAATAPPQLPLQSDLMTAQADEPAAAATPPASAAVGSSRPQMMFCGPNMMQPPTLSQFPGPFRSTRRWEVFLPDGPCGPPVSGVVGGAPVTPSGAFFSATARTTGASATATVGPGSSCRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0P7</accession>
    <name>A0ABQ5S0P7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045355277" evidence="1">
        <location>
            <begin position="30"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="697" mass="76966" checksum="650226826D499261" modified="2025-10-08" version="1">MNFYFETGWLLVICLRAPLLLAVFAFVGATDSQNYRSVTNGVNNLFANKLDLYRGTLPDDLRKRATAEAIGEHLRPTLRFLYTDVWQFVKEHDLVVPEFGHLFENEANMLFGNITYIRHHGGAFTFQEALQFLLATLRSFDPTRLTSILTAYPICRRTAMALILEHYLTNGPFQDAEASLIVNISQHLFKLHHRAAQTKRIIEFFHVSKSGGTSFCQLGKMNGCKTQSFGRQQNCLITYFRDSPRWTKAGVLGELSAKHGDPWCARYGRQYGMRWHCRARRALMTRLRFNFYSNELVMHDHNNSWTGVHPCREFLNVVIFREPQSRVISHMQNILKEYVNFYNESLWQAFNPNSAVQWRALAVPVFDNYVVRSLLGGKMYNMPHGTINATHLLAAKVVTLQFEVLLSLGPETSELTRDIFGLGLGWQYNLLHMHVRPTEQRAVDGFSREVLGAVRAAGRLDEQLYDFALVLQLLDAITFGIAHDVGGMDAVVASGDVTLAAVTDARAEAEVPGMNRTAASTAAAAGRRRRSRGCGYVGVRWPPGSVSTYHVHMPPVAPALEPYRLSDAELGFAIEGLGAMVIAGGRWFDGEFYRNPTAALDAAAAAGRAKEAAIVMTFAEAMAEQAAAAAARNRTKAALEAVEVTAAHAASMKEKAASLAEAAARIRSTGALGRGVGAIASVDGAHGAGGLGRKRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0S0</accession>
    <name>A0ABQ5S0S0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006477</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051292">
        <property type="entry name" value="Xyl/GlcA_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270:SF52">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE PROTEIN GNT13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046772821" evidence="7">
        <location>
            <begin position="21"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="478" mass="54012" checksum="F547EB6FCDB38A7F" modified="2025-10-08" version="1">MCPAVSLYLLIPLLLMLANCANIGVVGDDEANILKSYWNSNYNSTPLSFNEWISGHHARLYMVDAWWSQRNSSIHINIFTTMTLDRIHMLEALCQSYMGPVSAAVYISVDVETYPYDFTSSAGGRGTRRLKSARRRLGEGAPELAAPIADAREKLQDLFTRMEEQSTTIDMIAAALRSNNTPPCCCQLKMALMAERLADPHLAYLMPTNAMRNVAMLMVNTPLSIMLDVDLSFGRNFNELVQNSTWINGLLQGTHGAPQPVMYIPPAFEAKAHHSLNAAKIIVEISLQGGKQTVSSLWKLGILQAFQIDICLHCHGAFNHTRWMESDVPYTVKYTQGFEPWGILNRFLDPGYDERFRGWLYNKQTHVEALAQKHNFVLKVLPDVWAVHRPHKKIASRKLAKPVPDNVKETNTDVMEMLKVITLPDGTNASAYSSYRKYVDNLRYKARTALAARQPYEPQLNPQFLHCKKALPWWGGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0W6</accession>
    <name>A0ABQ5S0W6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="41910" checksum="524CB00054CD4712" modified="2025-10-08" version="1" fragment="single">GVGFAAAVAYEVAVQLQPPHPPPAGSSGGGGVVATVLLLLEDPRLRRCCEVASQPWFQLRDWVAGWRPDLDLTEFAMVGRLLEEQVNGRGHSAQMDYVGGLAPPGIPRAQWDLLAEEQARSANSRIGYGWEEMQYSWTVLYGMVEALHNKHCPQRDDSGRTSAAIATTTTTTTADSGGSGGSGGGGGSSSSAAVAPGAAAVAEFAALPAWEAFLLDLYALLDRPGAQLEYLVRYRPAAFIDEMSWDTTVRDLLWTASYLKSIAHTHKPAAPFLGETVFLHAAPPIASVISGSIRDFNAGWAAGATRSFALLVQPVRMVRLHDTSAPAVAGALADSSMESAAPSTDSPILALTTLQLQQLLSEAVNCYSRVAAIRQQLAMTTSTSGVPLLPLVPGSLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0X4</accession>
    <name>A0ABQ5S0X4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenine/guanine permease AZG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006007</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="XANTHINE/URACIL PERMEASE C887.17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337:SF1">
        <property type="entry name" value="XANTHINE_URACIL PERMEASE C887.17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="533"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="639" mass="68885" checksum="069E5C98A867BD5E" modified="2025-10-08" version="1">MAHEVAPSTERVSILDRLNYVVENSVVGKYFCIKERKTTFTTELRAGTVTFLTMAYILAVNGSILADTGGPCTVDDCSVNKGQPFCMFGSGGKFDAGYVACVAKAKRSMVTATAVASFVACVLMGVVGNLPFGLAPGMGINAYFTYTVVGFFGSNGMISYRDALAAAFIEGWIFLAISITGLRTKITTIVPKCVMLATSGGIGLFLAFIGLQTANGIGLISFESATLVTLGGCRVQDRAPMYTIADTSKVCSLEKDGTLAPANLGPASPNYACINNMRMRSASMWLGIMGGVLMVLLMARGFRGAIVIAILFVTFISWIPGHEATFLGAESQIPGGADRRQYFKKVVQRPDTSFTDLEMDFKAFGTTELWTALITFLYLDLLDCTGTFFSMANYINKRVPGFIHPITKDFPRMTFAFCVDACSIWIGALLGIAPLTVYIESATGIREGGRTGITAIMIGFYFFISMFFTPIISSIPPYATGPALILVGALMMENILDIDWKDYQQAIPAFLTIAVIPLTYSIAYGIIAGICSYLLLYLLLLAYDLLTAPIYRKSFKEILLDAKPEAFKSYDVLEAEELARNKARVEALERELELTNTTILHATSSIHDKGVAKVAAAAASAAGTEQVRSAETKPNEAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Y2</accession>
    <name>A0ABQ5S0Y2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Acetolactate synthase</fullName>
            <ecNumber evidence="4 13">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_006023</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="13">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="4 13"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0359">Herbicide resistance</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="16">
        <location>
            <begin position="102"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="14">
        <location>
            <begin position="296"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="15">
        <location>
            <begin position="487"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI63118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="686" mass="74910" checksum="06D2F1790B155892" modified="2025-10-08" version="1">MKMALRCGPVATSSHGCSSPIQRCGVLTPRQVVLPISFTSSTHQASSRPGRGCVSVSAKLADGSARRMQSEEVRRAKEVAQAALAKESPADWVDRFGTEPRKGADILVQCLEREGVDSVFAYPGGASMEIHQALTRSDRITNVLCRHEQGEIFAAEGFAKASGRVGVCIATSGPGATNLVTGLADAMMDSIPLVAITGQVPRRMIGTDAFQETPIVEVTRAITKHNYLVLDIKDLPRVIKEAFYLARTGRPGPVLVDVPKDIQQQLAVPDWDTPMSITGYISRLPPPVEESQMIPVLRAIQSASKPVIYYGGGCLDSRDELREFAARTGIPLASTFMGLGVVPAENPNHLQMLGMHGTVAANYAVDQADLLVALGVRFDDRVTGRLDAFASRARIVHVDIDAAEISKNKTAHVPVCGDVKQALRHLNRMLEAEPLPVDRFAAWRAEVAAKRDEFPLRYPQRDDAIVPQYAIQVLGEETKGEVIITTGVGQHQMWAAQWYPYTEPRRWISSGGLGSMGFGLPAALGAAVAFDGKNGREKRIVVDIDGDGSFLMNVQELATAFIEKLDVKIMLLNNQHLGMVVQWEDRFYKANRAHTYLGKREAEWHTTGDENDIYPNFVDMARAFGVPSSRVIQKDDLRGAIRTMLDTPGPYLLEVMVPHIEHVLPMIPGGATFKDIITEGDGSVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Z0</accession>
    <name>A0ABQ5S0Z0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006346</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045120171" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="221"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="382" mass="39891" checksum="C6B10C30820C59DE" modified="2025-10-08" version="1">MKNIIKSGGFLLAVLICFTVSVQADFMGGTVPSPTDGAQQLPSPRAPRIPRPPRSPAPPRPPAPPPRTRTTPRAPLSTNPPPVIRFVSSPPARAVLPPSPAPPSPAPPAPTLPSPPPSPPPPPPPPPPPPPPPPPPAPPPPPPPPPPTPSPPPPPVPPPPTPPPPPPPAPPPPPPPPPPPPGPPPPLPLNPPPPPPSSAPPSPAPSSPQTPSQIDDLVRNFPFAVCSARNITLSPYRLGPYLGPFNASRTENRYCIRVKDKGNVDPSSSCIDMVVNKVDFVVSRACVEGSPRPVRAATVNGISWSIYFSLRTYKGESYATLAISRISELFPKFPPGGLEVCLELRNASPCSRPETLCPGGKCVYALFNEDRTCCPTSQVPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S106</accession>
    <name>A0ABQ5S106_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006368</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF379">
        <property type="entry name" value="CALPAIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="7">
        <location>
            <begin position="82"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="441"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="441"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="899" mass="96428" checksum="D1B9E5583DA1C3C9" modified="2025-10-08" version="1">MGSREASPSRPGTSGGKVLRGRSDALVSVANRLRSPMYVSDRALPPEDVTVNTIEGLLRIPSLASGRTEEQLVASFCGEEGLVIDPDFPPGVSSLYPDVNQPPASAPSNVTWRRVSGTLYFPTGKTPRLLQGILDSGAFLGALAAVAGSRSGELLLDLIVSDDASPHGAYTFQFFKHGCWQAVVVDNYLPCVSGEERLAFACSAVVGELWPSLLEKAYAKVHGSYYALSGGCVHEALVDLTGGVGFKVKTDSLAGAAAIKEGCLWNDLQTWLSAKSIIACVARRKGPARNRFRPDAPPSPTGDGDGNGAHLQVEDPDGGPYGLQYNQVYSVLEVRAVGDAIQLVRLHCPWPRGMWNGPWALGSPQWDIPAVAPHLESFRTTWDDEATFWMPFADFAALFNRIHVCRMFPPTWHQLTLHCGWQGPSAGGPYYLQPHDPAFPATGAAPAADRSPSPSFPTGSASVSISGAGDRPTGAAGGGSGSAPVASAVSSTWCCNPQFRMTVRKACEVVVCLGQQDPTVSYRSHVPKRHRKRAIGLQVLRVPLESLGRRWEIRSGEAVASLPLSTGREVVASFKASPDYAYVVVPHAGRAGEEGAFILRTLSASPLEVEQLPAPLCLVVGGQWTAFLTGGNRYCPSWGSNPQYMVSCSQRAQVVASLTRLDLKYALMKAPRDPTRDLDLVLAHPEQGPDGPGRRTAVRDADVVAAAGQQQLGGSNEEVVMSVVLEPETPYILVPSISTPGVEAPYELRLMSGVPLELVPLPEMQSVQLPGEWTAETAGGCNVNPLWRRNPKYHIVLTSFGRVRITLSRVPSRKQRHPVDDMLGLYILRAAGPDGEVKGDPRRAIVAESTFVPQPDTTAEYELNGGCHYVIMPCTYGPGRLGRFTLSVSTATSFQFKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1Q7</accession>
    <name>A0ABQ5S1Q7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="18666" checksum="AC383C2391B01780" modified="2025-10-08" version="1">MLHVQAHAAAGADTAPAAAVVATAAVAVQDERIKAAVQAEASIADGQAVCGAVHTMPAIPYKSPIRRRTARIKIPFAEPEEIAPGYVERLQALVAQRGCICLGFTCAQLCPGGSLGPLYPDPKDANPITGTHGWEGERRFRGRQRNESPGTAAEEGDFKRLSHEHTGIKGRKGISLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2I1</accession>
    <name>A0ABQ5S2I1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PI3K/PI4K catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="55070" checksum="EB3D3DDC2D6BD565" modified="2025-10-08" version="1">MLAKALGFRHRLTWSNHPPMGRQGLFGRGLSHPALVSAAPVVDIGSVSTTADGLMQHLHERCTSADQPFCWRITSPGTSADEFQARTASLDERSASSTFPVNFQPASNTSSPQYRSGEAFGSSDHLQSQTFLRSGEKERSLVDGNSTVEVDEHCAQTHPGSVCCTSTAVEKDETHNSCVVTLRTTVKVTDSRLQVAPAELEELAWLAVRDTATEAVSVLNRQGSLSAGRQERHRFVCNMLRNGTIRDAKRTENGFGHVSYLVTLEDKGTGKRIRAAFKPRVEGDCEGWHRVPIEVAAYQLNLLLGMDLVPPAVMRGDCDVDWTHYPQGGAFIYWCAGPRQLNTVSMSDWSVPGPVLLSDTRILDVLIQNSDRHAGHFLYAEHWADGDYAPQPYGRGGSGGGSSFWRGRMSPVLIDHAAGFRQDAFVCLDHENAFLTGPTRRISARTYLRLRFLDAPSLRTALHGIITDEEIAALLSRRDAVLSFFDDLVAEQGYENVVLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2M8</accession>
    <name>A0ABQ5S2M8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007286</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF171">
        <property type="entry name" value="SD19268P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="347" mass="36603" checksum="C407C43405DCDF6F" modified="2025-10-08" version="1">MQAVLLCRSAHATSTWAGAASLTALLAVAASRASGLALTALTSSSDVHQRLSTSASYSSQSHGHSRSTSTVTVNAGTVGDGVDSGQLHVTVEPLSGVHESVSVISLSRPAARNAIGRQLLRELAEALDTVRQERTTRCVLVRSVVPGVFCAGADLKERATMSQSEAAEFVSRIRRTFTELQDLPMPTVAVVEGLALGGGAELALACDIRILGNGASMAFPEAQLGIIPGAGGTQRLPRIVGASRAKELIFTGRRVAGPEALDLQLADHVVPDDQVYNRALQLAEQIVRSAPLSLRQAKAAVNRGLDVDLHTGLAMEEAMYAQLLPTRDRLEGLKAFAEKRKPEYKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3J8</accession>
    <name>A0ABQ5S3J8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="16316" checksum="28CD36E5EB50FBB7" modified="2025-10-08" version="1">MDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTEKTPGHSIADATRAYVRDAKKYLQAVHAGDNDGIRCLFVGNTLEGSAKDWYREWTTARGDYTFDARDVEARKVLAASWGRGENVPACCATVHFPSTRRSTRSYEAKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3N2</accession>
    <name>A0ABQ5S3N2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">VaNZ11_007744</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10422">
        <property type="entry name" value="RNase_Ire1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.180">
        <property type="entry name" value="KEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045133">
        <property type="entry name" value="IRE1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010513">
        <property type="entry name" value="KEN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038357">
        <property type="entry name" value="KEN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954">
        <property type="entry name" value="IRE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954:SF6">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06479">
        <property type="entry name" value="Ribonuc_2-5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51392">
        <property type="entry name" value="KEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="537"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="738"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="domain" description="KEN" evidence="20">
        <location>
            <begin position="1007"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="19">
        <location>
            <begin position="1363"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="19">
        <location>
            <begin position="1404"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="15">
        <location>
            <begin position="1363"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="15">
        <location>
            <begin position="1404"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="616"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1258"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="616"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="645"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="668"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="691"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1279"/>
            <end position="1304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="GLI64471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1460" mass="152197" checksum="2782C597192F886B" modified="2025-10-08" version="1">MHQRVVGEIRRRWSEGRDIFFILLIMFCLLYHTRVGNANLTSKSRSIAEYRPGPPHSLDSLLLVALLGGDVVAVRSESGEVLWEYNTGNPLVRVSQGEAMPRGLHMLSGVDGGLYLYNSTLLAETASDSAAAADPILERLPISLPELVETSPSLTDDGSIITGERHTRVFALDRYTGDLLHVFNDRETGGGGGGDPHLRPPGAFGGIADVPVLHMMDPDRILLVGRQDFVVRSKQMLTDLEAWNATYSRIINLRPTGLGAAYRVPGSTGDGVNGMAGALPLFTVGADHTLQAYDPTSRLRRWALSFNTPPVGVFTMAHGDTNFLDPAAVQSLRDVDRVPPPPPTVAPARGQRHRGAPDGGRTLGMGGEAGQQNLALVGELKGSLYAMPSEHVVLDERVQPQSWSGIALSGSAPDSPLPLPLPLPPSANSAAAENAVVVHSSGGGGGGSSSKDLVTYVRDGATGKGVVSGLSCRSAGLKLLAIVEAAREERQAMLPDAPKPGRLRLPGSRAAAISRGGSSSPMIFDLAKGVAPAGGDMLMGVLLAAAGVGAAAVLLVMRLRGSGRVTSAAPSAAFPSGSKASSTLTAIDVAAAATGASEDGAKSDTAAATTATATAAARSVANGGPVPGRVRGPGSGRGRRKARAGEAGSSGISGSSNVIGGSDVGSINADTGTQASQPHLTCIPEDREAYPSLSGSGTAAASPTRSSSSSAPTASHLPSAEARVQLRGDGSLIIGRLIVGPGILGYGSAGTVVYEGVLDGRPVAVKRLLRQFTELARKEIEVLILSDEHPNVVRCFALEEDREFVYLALEKCRTTLSGFLETAAGRIQFVDPDTRQPTPRCLSTLMDVCQGLAALHERGIVHRDLKPHNVLLTESGGRAKLSDMGLCKQLVPEQSSFESHHGSGGSSGWQAPEQLIARDGGAARQSRSMDVFSLGCILYYCMTGGRHPFGDNHYERDARILRGDPVLAPLAAAGSEAFNLVSSCLAREPLQRPTVGAILAHPLWWGDERRLSFLVDISDRVENEDREPDTTLLAAMEAYAGVALSGLSGSEALDGSAAASGLSAALSAALSSAAVPNWGACLPAELVANLGRYRRYDFTSLRDLLRVVRNKRNHFREMPTTLQAMLGPLPAGFLRFFVGRFPCLLLAVYVFALQHLADEPTLQQYWGDGGNGAEPVVRTFMATYGKRMRPIKVCQQQQQQQAGTALAEASAAGVASTVGVAIADGEEPARNTAAASADAVPPSISSATAAMVASASSTNAGADSQSCGVLRPNGIDDGASSSAESTSAAAAASSTTASPTPLTQTTAASLPGVLATLSAAANGPHIPLPPVPALPPQPPPLVLVGYEPAGDDGELMVREFPRRPGKQLCDFYIKTGHCKFAESCVFDHPLEHAVPLTVLGLPLRPEEHICSFYLKNNDCRFGPSCKFHHPLLRPVYAGSAVVEGTAGGSNVGNKSQGDSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3T0</accession>
    <name>A0ABQ5S3T0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007585</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045591695" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="35"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="262"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="417" mass="44287" checksum="0E8DDAD143216CE5" modified="2025-10-08" version="1">MSSGRLLPWPRGRLGAWALLFFLIQISTTTMSLALASDEEPGGLPRQNANVRRFLLSSSRPRTMKGESAEEVEELRAYSTGAIARRKLAGTCIKVSGYVSFPNLDHAGDTIANSGKPAMQCLLSPACLAYNSAGELKASITPLTAAPGRCLYIKQAHLPTACANYTGFLAVPDVDHIDDNMTISTVSKAFAQCRSDTRCKGFNSAGWTKSAVSPTVPAKGLCLYIKITAETAGNPPPTSTSTSTPPPSSTSSTPFNSTAGCYDPAAALAEHNKDRALHGCAALTWNESLAKAAQAWAQLLADNCTPVSSVGKYGENLYILPSDWPLTASDAVCVNAVKFWYNGINSYKFTSTPWTDNAANFGNIGLFTQVVWKSTTQVGCGAALGNGNKCLAVSCRYLPPGNYIGNSQFFNNVPPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S493</accession>
    <name>A0ABQ5S493_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35046:SF26">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35046">
        <property type="entry name" value="ZINC KNUCKLE (CCHC-TYPE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="12"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="199" mass="22037" checksum="DE4E9DC1B4836B6A" modified="2025-10-08" version="1" fragment="single">GKGHGLLQPLPVPDAPWESVSMDFVVALPKTEGGYDAVFVMVDRLTKMVHLAPCTSSCTAEQTARLFFDNVVRLHGIPKNVISDRGGQFTSKFWEALCKLVGMGVNLSTAYHPQTDGQTERTNRTLGDMLRNYAGRTPLVWDTYLTAAEFALNNAVNRSTGKSPFFLNYGFHPALPVWRELEVNVPAAKLFAKSFMSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4M2</accession>
    <name>A0ABQ5S4M2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05325">
        <property type="entry name" value="carb_red_sniffer_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052184">
        <property type="entry name" value="SDR_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45458:SF1">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45458">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="250" mass="26938" checksum="2FA945E28B3DD657" modified="2025-10-08" version="1">MANPITGKTVVVTGGSRGIGLGLVKKFLARDNLVIATSRKSSEATHLHELHKQYPEHLILTDLDTSTTSSITAWAQQIKQQKVTKHVDVLVNNAGVYGRRLNLPHFEDEDFLFAFRSNTMGPFFVIQELLKQELLGGSPEAPGGKTLIANVSSIVGSNTDPTVSAITKGGFPYRASKAALNVISTTLARDLAAQGIEVVALHPGYVKTDMTGGNGWIDVEESTTGLMAVMESEAPLNGRFMSYKGTEIPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4W5</accession>
    <name>A0ABQ5S4W5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Jumonji domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008308</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF7">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(9) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjN" evidence="2">
        <location>
            <begin position="8"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="3">
        <location>
            <begin position="194"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="698"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="897"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="505"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="565"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="596"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="643"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="728"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="900"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="961" mass="101423" checksum="A79FB33A901C9455" modified="2025-10-08" version="1">MGDHGTVCPVFRPTREEFEQPFCDYVRKIFKKQPDLPMFKVVPPRGWRPRRTPFPDLRKVQINVPIRQHVFGTKGAYRCLFVEQKPMTVADFKLAAEEDAMRLRGGGSTHGTGGGDGSAGSGLRISSRTTSARYNRPPDYESSPATHQKVFAGADGECAPDGASTGGPDLDAVDPDLERAFWSNVTLNPPMYGADTPTSFFDDKLPYGWNLQHLGDLLQNHPKVDSVPGVTTPMTYFGMWRSFFGWHKEDADLYSVNFLHWGAPKVWYGVAPSAKLKFERMAQSLFPELHRACPAFVRHKDIMLSPACLKTYGVPCMRACQRPNEFIVLNAAAYHAGFNSGFNCAEAVNFGMKEWIPVGKEAVPCRCSALKDGVRISMKLFDPKWVDPYETPSESSQEEEDKDSEEGSEEEEEEDEEEEEEEDQQKTVAAKRRRGGARQASVPAAKKPRGRKPAAASGKKKKNTRGKKVVEESSEGEESSSSEEEDEVEEVPQSTKPKRKRMPTKKAAAAAAEKAEATAAVRKGSRAPAEGEAMTTSVRGKRSAATAVVKRAIAKSRGTADAVKSKASAPAAKPAAAAAKRGPGRPRKAVDTPEGANAAATPTAAAVSTGGRGSRKRKAVEEPEKAPAEAALAEGRGGRTRRAAAAAAITAAATVGIRKRARGGAAETIAEKDDAGASGDAAGGSEAVAVCARAVAGRRAKASVGQTEESEAAEAKDSGHSRGGRNSGRGAAASTPTATAAGTVMITAAATEEPPPAAPAPAAPVEVAIAEVNVEGRLVVTAVKPRSTVEAAAVALKARINTPCQESVSGPPMAIVGDADTPAGRRSTPAATSVASSVDKRFFFLVQRLSRPASTPGNVLLRWLKEGRDGLFRPVAGSVWEESAGALLPVRAQAVGPCGGATAADSDSDGDASGSGADVTGSSSPSHSAAGGPAAETVCEPEAWRLVTPRDQIINVELRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S507</accession>
    <name>A0ABQ5S507_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="156" mass="16598" checksum="7155396361766354" modified="2025-10-08" version="1">MSGERRVASSKYTHESGGYESEGYESEADMEDWDGGTAGEELHRPRRRTSLEEIVQPPPSQMQPLSPQTPQQPSACTDEAVGICPSCGDGGGGVDVGQYDGSLPEAQQPSTASSGVFVFYTSEPLPIGVVALPLRAPVQTWTSTCDILVLAPLRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5J4</accession>
    <name>A0ABQ5S5J4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Topoisomerase 6 subunit A/Spo11 TOPRIM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="104" mass="11651" checksum="D8EFAA6F9BECAACF" modified="2025-10-08" version="1" fragment="single">YKYGSDRLGPEGRAHPLPGLRWLGLRSGHLADMEQQDLQPLSERDRALMRGLWERLAETEPDWLLELTAMETAGYKADIEAVYRAGAGGYGALRDLVVRAVLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5W2</accession>
    <name>A0ABQ5S5W2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008702</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF140">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="191"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="727" mass="77896" checksum="C9EC62FC70BE24F7" modified="2025-10-08" version="1">MSTFVRLGLVAFSAGVAALVVALVMPYTDPRSLAALANHPIGAKVLNLSTSALHSFARLLEGVSPSASVPSLHHYHPLSANDSKAAQTATSRGAIASRDLDWADQRVWQLQQSQVTSPFYTEDELSRLLHWCGRSSSTNHDIGSPAVNYSRVVSSTATMLGREAPSSEEMAVNARFDRLARWVAASGGDASKVQLGVDGDGLRGLHANCSIGAGEVVLAVPLKVGLSAATFRAAQVLAGPTSAWRSFESADVFLDALVVSYEVAVHGDRSPWADYLCLLPRSYEGLPVHISDPWVASLLSELPALRRLVAKRRAQLALFANDLARPALMALPGGRSAMLRLARSRGDSAAEGSGGRDLWLPLWHWAYAAVKTRSVTLAAEDVRHGDGGDTLPGGQLPLRLPHSMLDWVQEYGMLLPILDMANHAFEGRGANVAYQLLPDASGTGLAAALVALRPLSPGQPLLFDYVSSAELEEGPACLERWLLEYGFVPNTADSDPARDCDTFEVTATDIARAVARTATSEPLAGTALTEMAVTLEHQIAERLSRRAMDGLPARLEIHVRQPYEDQQSGTQDHVAQRWAVLQWLKAALWDTAHEDGRAPESGSESKQPGNGARDCTYTGKGEEQHGGRTGFSLDLVNPRNVLKTLLQGRLEQLGELEHRAIEGTNGAPCTAAAEVGKRAGACASTLSGASRNEKLRRGLLAVAAAGRRAVQRALDQELKRQQTYGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6G1</accession>
    <name>A0ABQ5S6G1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ANK_REP_REGION domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052145">
        <property type="entry name" value="Mediator/Homeobox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330">
        <property type="entry name" value="HOMEOBOX PROTEIN BARH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330:SF19">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="893"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1061"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1141"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1329"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1395"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1482"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1538"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1675"/>
            <end position="1703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1797"/>
            <end position="1858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1958"/>
            <end position="2059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2169"/>
            <end position="2299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2375"/>
            <end position="2470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2664"/>
            <end position="2683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2850"/>
            <end position="2884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2991"/>
            <end position="3019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3157"/>
            <end position="3207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3499"/>
            <end position="3527"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="782"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="921"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1068"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1165"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1341"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1556"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1572"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1680"/>
            <end position="1703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1822"/>
            <end position="1836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1964"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1979"/>
            <end position="1992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2000"/>
            <end position="2021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2023"/>
            <end position="2040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="2226"/>
            <end position="2240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2275"/>
            <end position="2285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="2377"/>
            <end position="2388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2403"/>
            <end position="2416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2431"/>
            <end position="2457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2667"/>
            <end position="2683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="2850"/>
            <end position="2881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="3157"/>
            <end position="3166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3189"/>
            <end position="3203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="3512"/>
            <end position="3527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3527" mass="366060" checksum="95B60739F187FB90" modified="2025-10-08" version="1">MGRKKVAPAGGKQIPADDLLRLLHSQNLGKIRTLVKSGLDPNGYVSNTSCFDNTTWVLRDQKPLVPLLIFSCFNGVPAPAVDLLIRLGASVDAVTDCGLPAPLAVLCAKITAEEQRALFQVLRHHHCSTFNASMRSARNDLPIHVTCKILDLSDWTSPRKVDGIELLMQFGTLQLPDGSLDERAVYEFLATAVRHPCGHKLLDCIWRHFPQQLLPSLPVSDCLLLRALTDSWLEPVWRHSEERQHTVSMGLVLRALLEHPALQQPHREAVACWLRANMAVLLYHPELVALLLDRCKAPADSLSLRAGNEEKGQTAPLIAAAAGDASQSSPESCPDPLWSPLFFHPDDRNLVLPTGLLSGPEAAQGTMPLSPNMSLMLAYVVDADEQLARQGVQILLSRGARVDLPCRLGSEVLGEDCDGQITPLMAAFMPYHRNQGLVELLWQRFAETQPALATQEAAIQMRSAAHTRFLSLPGWQAPLDSAARAAAAVAMNATKADGAGGAVDCATTAVTNPGSLVIEATPLDCSRERLRDLLDSNVLDYVDNRNLELVETALTGLLAQLSSLPSQSAVDPHAATTASAAAVSAEGNPQGKGSRSIGGAAVDLAVQVAMVMERLERAQARLDDLYSSERDEAELADEPEVWRAEAEVESLSAVLRCLQRLAATAATAGTCYELPAGVAFVEVIAARIVGQPEWGFSRGLLLQLGLSQMRRVLRALQAGGGGAAAAAVAPPASGDNTAAGGGGVIAVGAEGGVQGVTAQMEVLRLYYDGLLSSMSVPERRLLEDLVSALEAAEEERDLEEVEEEEERAMLDLAAETALAAPTAAAAAATCPGATQGPLVEEEGVKEADSFPLVDWKSLEQRMPITKQSPLSLTRQQQQEQGRCQVLGQGQQRVGLGGSHLRGEQQQDQQGVQDTRPRPQLHRAESSDRKALGIPLQDEARKSSGVADGCGNTIHRLHPQTSACNASSRSSSRCRCSGSSSSSSSCVDARAPDFQANTAKDNADSDAIIAGRCKAVGAITTAYAISAYKPTDGADDFSAAVAQGFLSPQAREAMHRAMLPGESIGDWTSYDDDDDDDDDANHDNDDDDEEEEEEEEEAEEEEEEEDGDSAGSAGDGSSGSGCADSSVSSPWRTVVECGAAARSKGGGVTIRKVAGSSREHDWRGSAGSGGGSGGGGGSGVVNGTDRKGNMHGLRSGRCKRVSAQGDVGLGRKEEDLSLHLPPPQQQPRRHLRGRGAISSSGSVLRCRVRRLYSSGRSANQPEGFCTPMAEGWRDAKLLLGGLNVMSLPPNVRHGSGGGDGSCSSSYSDREFEQYEYMGAAIMLQNDSDDSVSGVGVGERVTGSPSCAESSSYDTPSEGSGDDDATSLLEAQVRACSNNGAAAAAAVSRRLRAVRDASSDDSYGIAQGRSGSGHRYGPEYGLESIGGDMGALEYGGENYPGGGGNGNGDFVRGGIGMGGASCRNKPLIAATSAAATCVDLATSDSTSSLLLPPPPQHRATSSSRRAGYQQPRRPRYGNGNGQRSKRDLLHRLQGHATADISLGDDAAAAGTKARPKTNSRDGDAMGVEWRDARSSATSDGGTTSSSASVNFMPRPPVAESNLLSLRTKTPTAPIPTAAAVIMATSTTDNGAQKVDTEGAAASAAAASVAGAYSGSGRVGGGRTRSVTRAVDAGLDAGLGGELTASPPLSRSSSSSSSSASGATGVSSCRFSLDSTRAYIVHKPSADAAGGESRRWYRLWRDSRELDQEAAASAPLPPPGRQNRFREVNPTLNRCGCDSSDEYVHTYDCSYDCVAGGGGSEEADSNVGDHQERWQGNTNSASGGRSDGSGASGGGGGLSRSGAVGCPTSPPGESWGNGRRWNGGIGISAGDGCGDGFATAPPVALGDMGSGAAAAAAAAAAVTEGENAIGPLTAAGNAGRPRRRVVVRRTHAASGPSAAAAVMDVPARNGGTDAAWPALRQAAVPSSSPPLSPPPRRTGPCSCSTASEGSGMSSSARERRRYSGSNLSYGSQGSVTARQQSTSGRVLEERRSHDGSNSSDDHGVSNGEDDVTDGRVGIDPCSDPHNRLRTGCCLSRAGHGDGHIDGVVHYNDTVRWHRPPDGLCDAGVKSSDDASSTSTQEWPPRRAAAAFGATNHPTGIPPGSRGAVAVATEAPIHTAANAVRAPAAEVVEPWMRTPPHNHRGLRGGDQGDSDSAGSVGFSGRGPEHGSDHGPSLVIRCTAITDGDDGGGGGGGGGGGGGGRISARWRDVESEPPLPRSDSDSDSGTAPPVNTRCWSTSSSDSSVTSRPTPATSGAAKRPGALPAMGAAAATVAAAAAQRRFTTAAVHDVAVDVGTVVAGAAASNARGVPRTVTAATSTTPGVAAASTTIKYLVNDSAGDGDGDSDADADADGKSPGVSGRRNTLPASPVALSLSLPADPTEPRRPVSARTSPAAPQLQHLQQQQQQQQRQQASPQAVPLMRAPPPPPLATATATVTATAGAAAISPPGGAERTLAALLNNLATSANVFPRPSWELLPKMGVVTGPGCGVVNNRTAAAVCAMKPAAAGSEGVRVPEGSGGGDDSGAGLQDCYVLHLSGTTPEEEGGYAAPPAAVMRALATGPSCARALYGWAAELRVALVAASGCEHQRLFVVADTPARLWPAWLSALRAAIVSAVLGPAFDTAGGGATKDERSCGRQSDRSTGRRHCPEWLRQTVLLLPLCHLIVPYGGAYLYQAASRACQESGSNPAAVASSGRCRTPRLWLDAECLAAAAAFAEAYPGNMYGRAILMVYANRQVGRALLRSPRYGLRALARSAETLAQVLYGSSGGGGDSGAAADGCGAAAAATAAAWMCGPLGQHPAAAQALLLLDEDEEDDDDDDDDDDDDASGGDDGEGSTTDDTDGDDGRVLRAASVTCHVLGSALNATRQMQTVWRATWPGGHSLRLASYSYLAATGYPRAMISALNWCVPLVLDGVADLSELLSEIRLLSFNVTTALIMAEEIFVPQHGDGASVRRAGGAESGCREGEEDEEADGPSNDQGSDTALAVKHLVNALRASVAPCPHPHPHQAGYMLQQKLLPWSQLCTTQREQLVDELLTLADLLMIAHAVLLTLRTALSYAADGGASATLAAAGALRREVAEVQMMRAAASRMLVERMEMQERSSEPSYHNHHHNYRNLQRQNRQPNQPNGLHDRQPISSTASNGPVAGQAAARDRDGEDPYSRDGGGDNGALCGEELHDWLDKGVSWRHVNGLYEHGKTVLQCAADAAAVLLDSEAREDDAARCDRLVTALHEDQHLLEGLNALATMPPHYKLPLAPWDPAAAAAAAEAAAAAASASGNGGEGAASDPVLQAAVNAAAAAASFSQCNEVLYRLLVLPWDDNGDGAAANGGDGDVCTSLAEEFERSSWSIPTLMKGDFKPLTQLRVRRLYEEVRGLAAECLGYLRRKALSDAGQQVTRGDVAGAPGDWAEFVSAKTFQGDLRQVGSAEDEEEQEQTVKAKGDLHHIAVAAANAAVVVVASGFEAGEDEEHGSADGGLEEEAEEVEGLDDPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6U8</accession>
    <name>A0ABQ5S6U8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="609"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="690"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="701"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="778"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="836"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="852" mass="87416" checksum="CF94F005AD42486C" modified="2025-10-08" version="1">MQDTNGNALESISAVSAPTASDAPSPPTSTPNLEDAAAIITGAVGNAPSSLTAALLAAPSPAVVPAAPTAMAPPAAQATATTPYGAAHQSQQTFTQDSIAGYLLQDPAFVAAVKRQDPMQAAQAAAQTMKLNLPAITSQVKPLQATALGMGAQHTTPPGMSYGTTAGAAAVSSGCLLSGPSIPAGYPGAMSGAVPPPGVGIGERRISDPDGRFKRRGSCEPVDLTGSGGPGRVRELRRGDWVMIRSANKSTDPELLGHDAWVARVEHNGWVELQVPKLGRTVKLQQRYLQLMTPQHPVMMGAPPGLGPPPTAAGVAAAQPPMGLPPPGRRATSDAGVGGMGPPSISYMDEQGRSYKRLQLDSSIRPALLLEALPPARVASTPGRGGGFRVKEVVRTIRRELLFLEEAVPWHYVTAAWKNARPGWRRCLQRNADPPVTAAGATAAGPGAAGAAAAAAADAAASAAMAALVGTTAASMLELHESLTTEKSQGLFARRGAWETRLRELAEGGNNHACLQVLWSEMAEGIRMWLAGGSAPSGTPGATATTPTVAAGTSMPLYSRSYDGTASERGGDAFMGQPLSEDDYYIAAAAAHQHHQSVQHLQQPSPFFDYATAAAGPPGSSRGGNGAAMAAAAASRPPVTPWMMRSELSVQLQSVEAEADTDLDEGPDATDVEGENGGENDSDVDPLAAARRRRRRRLQRRQQELISEHEEQQRKQQQEQPDQQPAPPADVEMPYLAAAAAEGEQDGGISSGTGTPTEAAALAERPPTEETDGRSQLEGADAGDGAAEVTATATGKTAAAASGLGIAEPMDVDSVVDSGTAGTATLPPPPSGLGNETEQVPGSTTKALDVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S798</accession>
    <name>A0ABQ5S798_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acid phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008850</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF207">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID PHOSPHATASE TYPE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046456467" description="Acid phosphatase" evidence="2">
        <location>
            <begin position="24"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="596" mass="63431" checksum="DBB0423951F43FF1" modified="2025-10-08" version="1">MANGWGPLAGVCALLLLLAGIWPQRESHIASTTQTLVDPVTARYGNFKPEDASLKLVQVVFRHGARTPLNTIYSFHDVNWTHCRDHYQGVEVELHDENGGAQPPPILDPNMPEQPGGCRQGTLTEAGYQMAIDVGTWLRSRYVAAGGLTGGFLPDIDPETLQETAAAKATDPITASEVASSTSWSSSVGWNAAVATLRTTPYRRTVATLRGVLTGLWPQLSPPSEVNRGQAASQPVLPVGASLESLEIMYGNNNTCPLLGPLHEAMQRDLDAADARDSELPRLNRQVAEALGLDPDKAILWPKLYDHLAAMIADGAPMPVGATREVLDIIWAQAERHEAGIIAPSVDLCASLGPGDTDGDSARRRRCHEVLRLGIGMLVRRLLDNLEAHVAAHTRSSEAMAAAATATAPLSQLYLFSGHDSSLMPLLAVLGQPARAWPPFASHLVFELWQTTAAAAVPGDKKAAEVAAAARMGRRGGVTAESELSGLGSGSGSGHQDSEFWVRVLYNGAPLVVPDLSNAAGWAPMGLLRQRVLLPYAITPAEHTAVCSTVDISDLDAARAPSRPTSTDDIRAGEYDSISRKAAAAARARLLRHATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7G1</accession>
    <name>A0ABQ5S7G1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008927</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="108" mass="11239" checksum="25EFD1C54A5A0844" modified="2025-10-08" version="1" fragment="single">EEGPGYADFSPPGARSDPRDIPRWSRSRFSPQRTAAASAGAPYGMPLLYYDEAAEAAEMQHSGLGATTTTGGSAGGSWPSHGLGEGFVSGGRHVLYCGADIPLEQLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7K4</accession>
    <name>A0ABQ5S7K4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Galactokinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_009539</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. GalK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019741">
        <property type="entry name" value="Galactokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006206">
        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00131">
        <property type="entry name" value="gal_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF7">
        <property type="entry name" value="GALACTOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457">
        <property type="entry name" value="MEVALONATE KINASE/GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00473">
        <property type="entry name" value="GALCTOKINASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00106">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Galactokinase N-terminal" evidence="8">
        <location>
            <begin position="48"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="6">
        <location>
            <begin position="143"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="7">
        <location>
            <begin position="410"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI65895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="552" mass="58111" checksum="3D542B12097B0497" modified="2025-10-08" version="1">MSAVDDMKSEGTALSFKPSPTVPLFTDLMDVYTDSPGLEDRFTQIATAFELAYGGKPGVYARAPGRVNLIGEHIDYEGYGVLPMAIRQDTVVAIRAAPGTDKLVVGNVEAAKYPTLEFGLDPEQPVDVANHSWANYFVSAYKGAFELLKSSRPDSVPEPVGLQVIVHGQVPTGSGLSSSAAIVCSSMLAVLSALGVPPSELDKAAVAEAACKAERYVGVTSGGMDQAISMMGQQGVAMHVEFNPVRGVCVVLPEGATFVIANSMAVSNKAETAPKRYNLRVVECRLAAVLLAMKLGLNAEEALEKIRTLRDVEPLIAEKYGAADGATCANAVSEQLGEQYGIAEIEEALGGRKLGDLFACEPGSLRALAVAEADKYSFPLKKRAVHVYSEAQRVLEFRTVCEDASLPSSERLEKLGGLMNDSHGSCADLYGCSCDELDELVAIARGAGALGARLTGAGWGGCTVSLIREEDVEGFLAALRDKYFKARVEGGTIAADQLPEFFFATKPSSGAALLKPDRTDGDTVVVETGEAEAEAPPVAEVLSVVMEDAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7X4</accession>
    <name>A0ABQ5S7X4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carboxypeptidase</fullName>
            <ecNumber evidence="2">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009262</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF254">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045013322" description="Carboxypeptidase" evidence="2">
        <location>
            <begin position="26"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="631" mass="68842" checksum="916C38D66F26CF9B" modified="2025-10-08" version="1">MVTKPREVFLLSALILQTLASFAGAARPQLAWRASGVTIGLKADAQPGTKFVKYCGEHADEICNLPGLNGVPHVRMRAGYITVSEISNRQLWYLVADREEESNDLQRAGPGDASSSSNVNGDVSGAAAGDAGYDRPQPRSEVAPVILWLTGGPGCSSLDAFIYEHGPFKFSYAASTATTANGGDAQAEAGEVAGDGVEGADKLSRQLQQRQRAVLLERNPHSWTKVATIIYVDSPAGAGMSYSGRPEVDYHTNDEYTIADLVAFLGGLTERYPELATAPFFLAGESYGGVFVPLLAQELLRVNRNRRADGEEPLVNLQGYSVGNPVTDDVVDGNAQLFFAADMGYVDPPSWRRMRAACDDMFWNATRDTECWDAQGVVKDDLWDLNWYDVLDPCSKPDDDDDAVMGNKVAKSGNSTGGGGRRQRQVSELERRFYWPFSARMGYPSPQGTASAVEGQAHTEAAGAGRRGGRQLWGPWLRHVVPCMDRRIALDWLNRAEVRAALRAAPLSVIGEWQPCSDVLYYRLDTMNLVPLHEDLVRQGLRALVYSGDHDMVVPHTGTRKWVYDKANLGSTDGPLRPWMLNGQIAGFTARFNAGAGFRFATVKGAGHMVPQSKPLQALHLLRTFISNQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S878</accession>
    <name>A0ABQ5S878_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="953"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="995"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1005"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1698" mass="172303" checksum="E6C915CB00EBCDF2" modified="2025-10-08" version="1" fragment="single">VEAAASLPLAPPQPLQLMADAAKLLAAMVAVSPGRVLQLLPQLPLLSSPGGDPLAGLPPSVAMDALPAFTVPPRIDSFCGLLPVLSAVQRDLERPTGQYPLTVALLSLLAELLERGFVGAPLPVAALFVMYELLPSHHHWHYESLKERWTVTRGSFRVLRLALTAGAYVTDPDISASVAPPPPPKLSSTATARGAVGAATGGGGATLLLTYGGDGGAGGGGGLFNTEEKGATLTPDDILQAAAPQLHVSLLSAAVLKLLLLHGPVLLARCLPPPADVLEQLQREDPSRPELTVLEECAVELVKIIPPLVAAAGMHPQEQPFEEYLFGGRDPTPAAHVVSYITYAEANQDMLPPQQQVSYFAMRALSSIAACVARPVSRSQPGISLALAVPVGSGAESCLCSLLRGTAASEHPELYAVATQLASEAVTNHPEVLDALLFPASLEHQQQGSGVGLPLLPAPPSAAGGQGAARQWAGRALDGLYATLQNAGKLKQEQPQVLACALHVLAAMWRQPTAASRPLSLLRSTSGLWRGLEAILGQVANDTSATFPASLSEAAPEASLQARAQGALCEAYALQILTAEAFARARLPAAAGATVANGSEAQAVLDRLVRGGGLLRLLRAAAEPVVAVAAAGSGDVGNDNSVEAEIRAVGSLQHLAAALELSLGSALWNTGGSAGADDVSIGPAIREELSGVRSLMDQGMVSKEDLQSACSHLWMGPQSPATATDPFEDVSMPQAWHTNPLRLVLQRQCVGAALINRCHVSTTDLAQLPGSPTGSVPGTEQLLSRSRLAALLPSDYQNIMEPAVGDVNTIYSLGEVLIARLQAVCALTSVLAQGGRLLPDGDATAMAAAALLNPPVPAGSSAANEPATPSAAAPSTSTAAAPAAAVGGLLTTVQLVCWTAVSGLVAWCASKDSRAALQSDWGLRRLHAQRVAAALTAARLLLSIMQSVQKTLAAPPPATSSGGSGGGGGFSTPTRPGPALSSFFATPIALTPVGAAPPRSGRHGTAGASTPTMSGGSGGGISADQAYLPFVASLARQWAVWARAVTGRLPQCPPAVAEQISDAIAGALLLALRPLQPESAVPMDQQLMQASHRQVLSASLRSTIPQLCDLAASGSISTSLAAQLLLETTRHLAIKDWLPQLFNKLDLGEYIRAAANRAADLATAAAASGGTANVATRGGADAAAAAGDLGMLSLAVAVSQIPEGAYMLYEQDIVENLVAASRQLLSGSGGRLASFSVIAVTGTGPYKPGVDGTYASAAGATAAGSGRMSGSPPPDTCGAYLPTPSPAAAAAAATAAEVTGEVWSPVHRQWCTLLQFCGSLVKTLGQRLNIDRGALDLLMAMEPRLMLAVLPPGSDVRQPLTLAGLVEMEAALFLLIQMTPYTGGWHMLLPSSLLNFRTAVSTLLTWLAQPNLVKSFSVDCRPRTAREAGLAGVPAAGIPCSDGWFRICSLGAASCTTAATGPASAVAAMGGAGGLGSPIFGSIPSVGSLSRGLSEGLPVTTPGLVTPIGATRTASGLGMATPGGPSAGATPGASGGGGVGGMGGVCSEYSAQLAEKLYTCAQHALTFLIASSPQLSQGEAAALGPAWPRPQDLSALLDQCMCASESLAGVVATAMDVSGAHDRRTRLMHLLGRVGQLCSDFLGVLAAAGSSNAFGAVASVAVREQLAREWLASLQAGPLGASVGGFGAGVGMHAVPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8C7</accession>
    <name>A0ABQ5S8C7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ammonium transporter AmtB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_009946</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="16"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI66183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="461" mass="48369" checksum="610C0021392682D6" modified="2025-10-08" version="1">MDSVAVQSAPFSDLLILHGAIVFFLQVGFVSLEVGYGRSKNVKNILLKNTVNILLCAIVWWSVGYAFSFGVSSGGFIGTSGFFHDGSLGVVKIWFYSWNFCLATVAIVSGCLAERTSLVAYPLLTLVMASWVHPVASHWAWQRDSWLLGLSNECRFLDFAGGAAVHLCGGLMGLVGATIVGPRIGRFEEGRAKDMPGHDVSSAAIGTLFLWFGWFGFNCGTTYVYLGNMAAAAAAAGVDDGLSETSSPPPPAITGTGGSNPADRVALNMTLTASVSSLTALLFSSLRSGTVDLVVCCNALLAGLVMSTPACGFITSWAAVVYGLAGAAIYLGGARLLVRMQVDDPLESSVVHFACGMAGTLLLGFLARPAYVAQLTGYNCGGLVYGARKGGLLLGLQVLGVVAVAAWTGVFSVLVFWALRRSGRLRVDQVAELAGLDNMDHGGPAYPEFNLVPYNNGSGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8G4</accession>
    <name>A0ABQ5S8G4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15175" checksum="667E967D71F9869A" modified="2025-10-08" version="1" fragment="single">MSEQLAMYRRCRRQYPDTAACVLLPAFCRRKAEASLRGMRHVHRFEPGTVLFNGVDREGTLAPWPHLSYSVDVWYEAPDGGAVGYGPALSALTLSAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVAEVNALGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8I2</accession>
    <name>A0ABQ5S8I2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sm protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_009647</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the snRNP SmB/SmN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01717">
        <property type="entry name" value="Sm_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017131">
        <property type="entry name" value="snRNP-assoc_SmB/SmN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037187">
        <property type="entry name" value="snRNP_SmB/SmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="4"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="147"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="177"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="232"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI65964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="273" mass="28055" checksum="8F8F35CA23AB913F" modified="2025-10-08" version="1" fragment="single">MALSRTSKLLQYINFRMRVTLVDSRQIVGRFMAFDRHMNLVLGDSEEFRRLPPKKGKSEEEREERRVLGLVLLRGEEIISLTIEGPPPNEDMRTERSQAAPGGPGIGRAVGRGMPAAAPGQAPAGLAGPARGVGGPAPGMMLPRPQVSAPPVQRPGVPPPGMGPPRPGMGPPGMGGIPPPRPGMPPPPGMPPGMMPPPGGMPPRPGMPMGMPPGMGPPPGMPPRPGMMGPPGMGPPPGMPPGMGPPGMPGMGPPPGMPPRPGMPPPPGMPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8Z0</accession>
    <name>A0ABQ5S8Z0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_010179</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="193"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="32"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI66387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="413" mass="46287" checksum="E2F9BD25DF6C8969" modified="2025-10-08" version="1">MAAVDVAPSKADDMETDAPLLVADVSTSTNADEDLYTTLKNLQRQLEFLEIQEEYIKEEQKNLKRELLRAQEEVKRIQSVPLVIGQFLEMVDANSGIVGSTTGSNYYVRILSTLNRELLKPSASVALHRHSNALVDILPPEADSSISLLGEHERPDVTYQDIGGYDMQKQEIREAVELPLVQADLYKQIGIDPPRGVLLYGPPGTGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPSIIFIDEVDAIATARFDAQTGADREVQRILMELLNQMDGFDQTVNVKVIMATNRADTLDPALLRPGRLDRKIEFPMPDRRQKRLIFQVCTNKMNLSDEVDLEDYVSRPDKISAAEIAAICQEAGMLAVRKNRYVILPKDFEKGYKANVKKGDTEFAFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8Z4</accession>
    <name>A0ABQ5S8Z4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Polyprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009990</name>
        <name evidence="2" type="ORF">VaNZ11_010008</name>
        <name evidence="3" type="ORF">VaNZ11_010016</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="1 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000029">
        <property type="protein sequence ID" value="GLI66254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000029">
        <property type="protein sequence ID" value="GLI66261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000029">
        <property type="protein sequence ID" value="GLI66272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="14438" checksum="C53A3BA5F4060687" modified="2025-10-08" version="1" fragment="single">TNTHMQAHGQALDAADDYIFLNDEDDVHMAERMIRFEEPVQAESVSAALEPPAIAPAAIQPTTPTKLEERLHYSYKSRGSKSTAISAPCSWGIRDTFVEVFGSRICGQASIAAPSKSVFVKLLEAAAGQPSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9Q0</accession>
    <name>A0ABQ5S9Q0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="44738" checksum="64A8545B311F2731" modified="2025-10-08" version="1">MSAERDDTPHTSLASQPDFVVAARAPDRGLETIGARATQEPLFLQTPAHLIVPHSAVNTNMDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTDKTPGHSIADATRAYVRDAKKYLQAVHGGDNDGIRCLFVGNTLEGAAKDWYRNWTNARGSYTFDELVAALLARFATEVQARDVEARNVLAAGSYHMRRDENVPAYQSRFEALVTPIADLTESERVFWFRRGLTKLLAKWCATDLVGAQIKSYDALVAFAKGAEIRLLAGEEEVKSGPRANAVQVQDPLGEAVALDQDLGATRPTPTAATRRSNGQRKARGGASSGVRGTGGAGPSSDPARNRKRGGAPSSAAAQRSDPNVKRTRTDDTFMPKWNCNRSEFERRKLANVCLRCAENHSTKDCPLMPPFVPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9R2</accession>
    <name>A0ABQ5S9R2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010586</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05467">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="3">
        <location>
            <begin position="200"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="CBM20" evidence="3">
        <location>
            <begin position="349"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="CBM20" evidence="3">
        <location>
            <begin position="538"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="71"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="427"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="671"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="736"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2474"/>
            <end position="2518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2533"/>
            <end position="2624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2655"/>
            <end position="2830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2852"/>
            <end position="2880"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1221"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1558"/>
            <end position="1625"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1709"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1810"/>
            <end position="1844"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1897"/>
            <end position="1992"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2034"/>
            <end position="2061"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2085"/>
            <end position="2254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="685"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="740"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2603"/>
            <end position="2620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2655"/>
            <end position="2667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="2670"/>
            <end position="2692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2693"/>
            <end position="2707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2716"/>
            <end position="2729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2779"/>
            <end position="2791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2869"/>
            <end position="2880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2880" mass="302083" checksum="9C36A8983DDDB7EE" modified="2025-10-08" version="1">MIGASFIAGGERSCTNSNTFNVVPTPRVYISLKAASGNPFKYKKRSFDGFTRQTFSEGFRLSSASSKTLLVGDKRRRGVPCRAGRDTPDSRHPGASDTRSVAASASTPAAAAATADCSSSPAAGSQNSLHPSDGNGDSFASPHGTNTSTSTRDSNSKFSRTDDVDPAASSRSAASIAANGAGSAATAAAVAAALSAAPASPASTMVCITFVVPSFTTQPGQHLAVVGSCGSLGRWQPGRGLALQWGEGHRWTGGLRVALEEARVAEFKIVMVDGRTKAVTWEPGRNRVFKVDTPSAASSRSVSEPPHQMLVCEWGKDQSHLKVPSAAAPGGEPQGGAAASSSGGGGRAPPSCRLARVQVVVPLFVGGPGQRLVVVGSSPELGCWDVQRGLELSCSPGHIWRGEVELPLTNRLEAKLVVLGPGDGVQRWEAGPNRSLSLQPPPPEDPLPPPPPPPASFPPTANEAASTSTSTCTPTSPTSAGSGEVAAGHYVVLWHWGITGCVQALYCPSSPVNRASVSARGGAGDGDVVSGGTAAALSDQQVLSLVHFTVPQFVTAPGQHLVLVGSVPALGAWSPAGGLQLQWAPGHCWSAEVLLPKNQDIEAKVVFFDNGCYSWEAGPNRLIPLSRVLADCSPGAEPVFTCYWGNTPTTPVVVVPKRYARDLPLEHQQLEVEVEEESEHEHQQLEQPEPQQQLPMTEMVVGDANDQVGEGKLLLQFLAFMVGTFIGDVVTQPTAGVGTSSSSGSSSGSSSGSSSGSALSSRGLTDGAAPAMYASQLAEAHKALRAARFRNTKLAWELAETRRQYEARWSELQQISKDAAAKTKAADVDVAANGDDNGNGNTGTAAVSLHDSGVAVGTAAGTLERFGGAEQVEGGFPLLEPAVPPLPEMGLQALAALLSALTGRVPPSELQQLRGLLRRAECTALEFEVLAQRIGATPSVLEKKELISGEVVAAEAVAALRARLEHLVATGGAQVAEAVTRAAAAATAAAADAAAPAVVSALSPALLTATGDTTALIGERSGGASKQLQSHLAGVGAVAAAAAVAPGAPAAAAAEVALRNVAEVAAALRRQLEAAQEQHRRELAAAQEERREAEEWRAAEVSRLERELEDLGERHATAMAAAEQAAAVAAEAKAAAESLMCDLHAAAQSAEAGTAAVAATPAMGSGFRAHEAELLQQLEMERQRLAEVVSERHDTIAAAGAAEETAAGLRAKFYVREAKLLRDIDKEREQAEYAARRAAEEAAVRAEKAERRQRELEEGRESARRQLAAAREALHLQQAAADAALSRLQHRAAAREAELALQLQQAAADAALSRLQHRAAAREAELALQEAHLHQEALIAAAAAQRAETGATVEGLAVRVAQREQELSELGQRLEVTRGSVEAANAASAALGCNYEDLAWELSTAIAMDGLDAERDASATVEAKLRAEVAGLMSRAASLELQLTEARRQYDNDVLAAELLAVAAREAALVTAQREFEMRSTAMLAAYEAELSAARMRESELLGALQAMDLDCKNEVEDGQVAAEAAALAEASGLAEAPALAEAPALAEASGLAESAALAEAAAVRETLEKQLEDLRAEAAARLHAARAELGVQRQQVEAVVAAAAAELASARDRYEAALVEQQKRLLEELVRSREAFVATSEAAARMRRESESRMVQRLAELRAKLEIAALEREQALRERMSADLSRAAAAAYDDVSATLVEQQLAAAAAATEVERQALSEEASRAREAYEEMSARQQNLAEEHLRRELSALAEAHAAEVAALEALHVRQLAEQAAAMEEVTAVLNSDILVLRRELNQRQNMYAALVADQRNLAAAASAAQQRVSELERQLTRAEERYGEELWKLTAQHDARLVSELAAARAVNEEAAGRNLERLVAAQEAAASAMAAQVVESAAALVQQMQLTEAQQQRLAEAEARQRELEARLTVAVEELAEARQSYDVKLAAAEAELVQGRRQLESQLVDERSRMLSEAAATRRALEQQQEAMQEEQLAAASRFQSQLEAALGHHKAREEALSVAHQRRLDDVESTFREKIEVLASQLDSAQRALREAEDREKEEERGAEGAALRKQVEVAEAQLVAAGDTAAALRRTVDDLQKKVAEANMALYNERAAAAAQAVQQLTDLRGQHSRELQELRREAAAAAAAAAAAEAALGEAQCAADAMVSELRLQLEEVKRVAKEEQRAKEQLRALLSSKEAVVSELMTQLNAAQAAEFEARNSVEELSLRLVAAQDALAGAEARAAEAEQALAATRRDAAVEATEAAAWASAAAIQVAAEAAVVHEAAALAALEQRLRDEHAAEVQVLQTQLSELRTHHAAQLALRDAALHKAHVEAAAKLAEQAARLWAQFDYSTGNVRQEANIRGMYDLEMLRQQGEADAAELRPGFGAPIEDGSRALQQALRLVAALQVHVRELQSQVDGAAAKKTAANIAAITAATAEAVHGAATAASGNAVPFSPLPTTLLANNVVPSLSRGLVSSGSGDIGADRGGDRSAAVASSKPSDGQRHGGSAPRGGERSSPRGLLLEAEVEAEADAATVAAPSGSDRIASRETQPMATVVAKEAPGGKPARRGGSGGGLLNDLVRFFRGAGHRTDDATAPPPPGEPSVSSSLSSATAMAATSSRQVPRSFAVSDVVDAGSTNNTLLAATLYKEMLDRSRSRGDPHIREKPLASSPPPPASIAPPLAMMPPTPTPTPTLELQRQQQRGGLRSPAASGRGKRQEENSDGAKESDGIASGSPGQGEGEDEATGTGGVPTDVFVSGSKEGTAETAVSYMTRGGGFSRSSAAQQSRPISGQQPSGRRRRQHQREGQLPEDAVDEAQLQGADRAPSAQSVYTWAAVVAALPMAIAHTLYEEQQKQLEQQHNGGPGPDADVREDMRGLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA83</accession>
    <name>A0ABQ5SA83_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad21/Rec8-like protein C-terminal eukaryotic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010347</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="2">
        <location>
            <begin position="121"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="166" mass="17299" checksum="B5909E73667C5639" modified="2025-10-08" version="1" fragment="single">AGGNSKGGGRSEGASGGVSRKSSHQADSSARGGSGSEKEAADATPFFCLKETNGLATQVLPPQLFMSRNTKVVISIFRKQLAQAQSQAPVQVAEQHQGAGHSNGGDSGISDLQEPALSFFGLASRLKRSEAVQLFYQTLVTHTAGYVVASQYEPYGDITIRAAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCH2</accession>
    <name>A0ABQ5SCH2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011422</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="19887" checksum="69C30BF8506ADA5C" modified="2025-10-08" version="1">MDTFGAPREEGLRRALSLAIPVGTQLGSVGLESLGRAADGTLKLATWSVTSAVRTAGAGAAFVNRNLFAPLSSLAFGSGSAMASTTNIQFCTQTCPQLLIQKGRKHNPSEVRYQKAASNLRTGSAKCSIRRQQQLSGSSFASQQAELLAHLAASCALAPSSDLQDEPCFGKGDVAVAQWTVNALGLQSSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCL2</accession>
    <name>A0ABQ5SCL2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Fibrocystin-L</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_011387</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
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            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
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            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
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            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="CEMIP_beta-hel_dom"/>
    </dbReference>
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        <property type="entry name" value="G8_domain"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ig_E-set"/>
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        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46769:SF2">
        <property type="entry name" value="FIBROCYSTIN-L ISOFORM 2 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46769">
        <property type="entry name" value="POLYCYSTIC KIDNEY AND HEPATIC DISEASE 1 (AUTOSOMAL RECESSIVE)-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24606">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IPT"/>
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    </dbReference>
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        <property type="entry name" value="PbH1"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
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            <end position="21"/>
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            <end position="4740"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="1649"/>
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    </feature>
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        </location>
    </feature>
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            <end position="4141"/>
        </location>
    </feature>
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            <end position="4431"/>
        </location>
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            <end position="4517"/>
        </location>
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    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="GLI67174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4740" mass="500633" checksum="B483B61B64068F2F" modified="2025-10-08" version="1">[encoded data, 4740 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCT4</accession>
    <name>A0ABQ5SCT4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
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            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
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                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <source>
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        </source>
    </evidence>
    <sequence length="2020" mass="211971" checksum="A1C63B8F52CED394" modified="2025-10-08" version="1">MFIRRRREREPTRWYRLADSDELFTALDEAPNDHPLLPASGKMAPPLRRFVPMQRPESQAAWSSQAARARQVHPLQLATSLAFSSSAETMMRPLAPPNRYNEDQYNGLRFKDMRQPQQPFVQLQHLLPPQRQHPVFQQQQPQVLQPLPQPLQPPGPRSHQQETPMWATQLAEHGGRSSPALQVPSGSRPWQARPQLLTAAPQMAPVLHGGGVSRVNVTAAASPMNTGAVYRSPTVAAGCSTSAAQPLKDLPPPAAVAAVAAIEISGGRNGVAQSLQTTGTYSGPAALQPTRDKPVQLIGPYLIHPQWHEPTYQQAVGSQGPVVWDAPPLYLGRAPLMQPVPVDLSSAALRPVNASGLGPPDMSSISSIQQWLLLMPDHSKMALEKAWFMSVERATKAAVGPSFEGMGRPPPTSGDNRQGNSALWQPVGGRPLEWKATGPTTQPGNLAMKAQGMYHPDGTALTPELVLHYTNLLYGEDWDKQLPWQAATPVLTNMGTRVPGAYLGHSPDQRTANTNQSPVASAPGAKRLYNMYNQLLGRVITPPKLVHTAIPPAAGQHDSELPAATGAATKQNAQPLACLPYMPELMEHITKATLSRDAPPSPGAIRYIPKTAVWQAAWAAHRAATNTTAATAQDLQAAVLGTDPGAVGHTASNNTLYACEQMATKIPALGRDGTGSVAHDGPIPVQHQTKQGASRWRDRHHMESVLAPVFVVDLDNCQVAITYIHGHGRSEVQTQTDRCSPKDGAMMAAGDSSDATIYSSHSSVIGSGTDMTAPSSSSALTSDDIEDSESTSEGIDQQPTHVTTSRIAQLLKSMNIFGRWRHRGGGTWGMTRGLKHDPPARQGQAAVASGDEIPAGASSVRIMRHEDDSVGTTTGDALTAAAAGKIEAASDEDNKANTTNGAAGGRKQRRKRRKTEEPQTCSTGTSGVYSAIATGASGIDHQQKQIGSRQQKTKEMVKNVQETRKAADAGLKGSASDAGDGAQRRIKHTKSKSKADTKPPAASTAPNIVVSTSTAVGDRQEDTKSRKSKSPKAMPSMSVAAITDAAATTAVHTAENNDGTMPPVNSEEGGASSEKVDDDHHSVATPDQAGRLMSHAGIRSKVRHVVRRFNLHFWKQSGSSQPRKATVDRGLINRTESADSDELVGEEQQPAVQQQESSKGVATDPPEVAASVRKASWTGFHFFKHRKSKLKKHGDSSGPKMEADNTDAQDVNEVKDNNTGGNNVGENDKDAGLPASGQTVSIGANAAGSNSNGSDGVSKTVHTPAPSGSGNNKTWLQRLYNSVIGTSVLGRLPGAKEQPGIITGSPPSNVKPDGQVHNKKKGLMVADELMQQSPGEAAVSSRGGNLATSSRGQHSTANADADAVTENEESAGQVVAATTDELVQDLAEVENQEADDLEYEETSISQKSVPSGTAAAGKAMSKKGKADKRGHAKNGGNGDELTMETTSKVKAKIKAAKGGLFARILGWRHQGGTVNISQQVPRTNANTITGVTAGRNGGPDIAVVAGADSEKVTNTEGTAAAPAAAVADGGDEVRSQRAATETTFEPVGPMSAATGSTVVPPAIDLHAEPEVQPLLKPAASGEIEEMASEGLQTPAHVSTKVVIHGKGKEVKKAVRKAAGKNLSSTAAATPSIVLPPPQGPKAKLMQKLLRLSDAVNAQRAAAAASSLGDDVSSRVPASATTAAAGDDNAAEPAGRKNDSTVHVGNISDDTSQNLSNKDEPNEPKSSSGGLQQQIVQLTAETTAETPIGVSVQNKSKALERLKNTLARTRGRQASSTASGNTNGDHAQDSGQDSSGLSHVAPQGQQQEQVMWGTRSQHGNPAADNETDSSSKPASGKATMMERMMASLRRPKHQAEVQEMVVFTDGQNPKAVGAAETNGNTTSGAAQAAGADETTGVTAMRSSVNNAITASSIPAAVQLQSSAVRGSSSPVSGAGSLNTTSRLNSTTRSDLGASSPFGRQVHQAENLMASIRKLQQTASTEHEVNLNNTLGRRVGTAPGSPVTSGGWTVQQVSSNSGRREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCY6</accession>
    <name>A0ABQ5SCY6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PsbP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1570.10">
        <property type="entry name" value="HemS/ChuS/ChuX like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053733">
        <property type="entry name" value="Heme_Transport_Util_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144064">
        <property type="entry name" value="Heme iron utilization protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="17550" checksum="5859DF2509922D47" modified="2025-10-08" version="1">MLASRPRPFTSSGLRRAARPPATALRLHAQAGVTVRSLLDDAANAGLGKVRFIVLGDGAILESVNIFGSVRYNEVPSRGVLATVASEDKSFECHITLSKVKEVKFVKSKAKSGEYDIYATRFLGDDSKVLMSCLLHGQQGQYDPAAVAAWTDLATKYGESVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD75</accession>
    <name>A0ABQ5SD75_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011341</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011865">
        <property type="entry name" value="CysT_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005667">
        <property type="entry name" value="Sulph_transpt2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00969">
        <property type="entry name" value="3a0106s02"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02139">
        <property type="entry name" value="permease_CysT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406">
        <property type="entry name" value="SULFATE TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406:SF8">
        <property type="entry name" value="SULFATE TRANSPORT SYSTEM PERMEASE PROTEIN CYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0764">Sulfate transport</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="175"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="390" mass="42624" checksum="A2FA399CF2310940" modified="2025-10-08" version="1">MDRLASHQHATSLGQHHQSRPVKHVLQCINIRHSYAQIGQAGLGRYRRQKLRLSASAAAAASFEQPPRGSPPSVSLAESLEPTASSGAVAVQLSLGPAPSPPAASQSSASPFDSVQPISRFRLPNLSAPITWTYMLSYMAFMLIMPITALLQKASLIPISTFIARATEPVAMHAYYVTFSCSMIAAVINCVFGFILAWVLVRYKFPGRKLLDAAVDLPFALPTSVAGLTLATVYGDEFVIGQFLQRLGIQVVFTRLGVVVAMIFVSFPFVVRTMQPVMQEIQREMEEAAWSLGASRWKTFVDVVFPPLFPSLLTGTALAFSRALGEFGSIVIISSNFAFKDLIAPVLIFQCLEQYDYVGATVIGTVLLFISLTMMLAVNQLQSLNQRFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE59</accession>
    <name>A0ABQ5SE59_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012117</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="84"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="118"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="856"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="890"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="924"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="958"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="992"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1100"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1134"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="229"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="308"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="498"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="556"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="684"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1230"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1284"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="530"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="686"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="703"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1284"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1304" mass="139432" checksum="A024D14EB74AD47B" modified="2025-10-08" version="1" fragment="single">MVSEQRSPAPNGATLARILPGGNGRRTITRDYHRLINECTEKLHSNPKHTLAMRLRAQSYRMLGEDDAALADYNSLLALEPTNVDALFYRGTILERQQHIDEAIHCYTAVLQQEPQHHKALYNRAVCFSAKGSFAQAIEDFSAAIELDQQTCKGARRVRDQDPAQQQLASSLGATADAAAAAALHACSPQPNTLPHHVLQQYAAEGHALSSATSVSDVSAAGWERLNSNTSAWSTTPQSTGSDALGDEEPGTGSGSGASATVMLYDPYGAMQAGATAGLLAQASPPSSQRCPEGPGWASCGTGAALATARSHAGAQPHPHASQPEASVPPVGPKQRHHDLHHPDQHGRRYRQYRQRQWASGQGEDAGAVSEQQQQQTPSVHDSLGSWLEPRQSGGSSEAAWSQGADVAPAATAPPPPSQQQQQGSVATTPPPLPGSSTSAQLLASLQDSKALHMHQQAQRQQQQQQQQAEDEYVLEQLQNMFVAAQQHEQQAALARQGGQQQQPFHPQMHSVPTNGLRGGPHGVLREVQGQGSSNSRSQTPKGRAAVAVQRVAAAHVHPHMHARAQVSRPQGSVPGRANHLHQPQRHVQPPSRNGMTRTGPGSAPPAVARELRSPSPFERFVQDDRTSGAGASGAPRPTRNHMNGVNDRHGTPPAVLSTQPRPNMGMAPQHPQLQQQLLAQMVASRPNTASQQQQQHTTNSRCPRDQRGDGADRPCDSADDVLPEGQVQVNAQSEQVLRLQSWGQRESLQIKDAAASATTGALTGLHACSSGDLGEGSEALTSCGHVGSGGIGDSNSGVTETAPVDPGGDDRACSLDPWTVGGPGGMVAAMEMSRPSTSSSAASDAADQSSRHKAIEQCNARAFALRQQGKLVEAVREYNRALELDPCHFKTLFNRGFTHDRLGELAAAVDDYTAALAVDPQSSYAHYNRGITRDRLQNFAGAVEDFTAAIRLEPNSADFYHNRGFALRKQGMFEAAIQDYTTAIRLNPNHCRAYYNRAFCHDRLNHVQQAIDDYTKALEMEPNNATALHNRGSLHERNGRWVATKSDLALQDFDRAIVVDPNCAQSYNARGVLLQHLGRLEEAQADLDVAVALMQPPSASLFRNRAQVRRERGDHYGAVQDLTRAIALDPLDLLSYSRRAHCFRRMGEYESAIRDYTRAIGLSPNNPKLHTIRAYCYAMIEWYGEAVADYDIVLSLEPGNAHASYNRSIALEKLGGAIDRAAAELSAAAGGTSAPADRARQSVPAAATHAGQGRTQGQSAVAVPEQPTAAYALTAGNNVAAATAAAGPAVTPGNDSSGGGAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEX8</accession>
    <name>A0ABQ5SEX8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012901</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46699">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE STN8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46699:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE STN7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01846">
        <property type="entry name" value="FF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="54"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="430"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="543"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="688"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1215"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1326"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1439"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1404"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="435"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="543"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="578"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="703"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="728"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="763"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="786"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="797"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="811"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="848"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="905"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1234"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1326"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1337"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1453"/>
            <end position="1469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1489" mass="157599" checksum="DC2D1C532A2B5E7E" modified="2025-10-08" version="1">MRTPKCHRLASHSSLKNRSVLVASRLPATTARRCSVRVVGDLSSRGRPILPKSITVERIMGEGSFGQVFGGYLSTSSGNQRVVLKRVKTRVQGAEEMSQMEHLLNVYASRVARGHCADFQGYCEVADSEATGRLTPGLWLVWKYEGSKTLSYYLRRRDTIRALATDLEVPELLVLPTVMRHIFEGLAAFHAAGLVHRDVKPLNMILAEDVRRFKLIDLGACADLRTGTNYVPEESILDLNFCPPEQFVMPTDSPHIAKQAGLIKMAISPMLWAKHKPDRFDTWSAGMVMLQLAIPATRTDRALRNFNAAYGPKYKYDLAAWRKGTHMSSRDCALLDADDGAGWELLQSLLAPRHIQVDDNGGVSFVNDTPFQRISAFEALKHRFIKVALETAAPAGASTSGSSSAAAASSSFSAAGPARNRGRWLGDVAAAAPTSGGSRSADASGSGVSAPRGVDPLGSALGMWRDMTGRLFDLEARIMRQTSATELQTTTVRRLKEQLDSGKVDPAQLQKEEKVLSKMQSRLAGLQQDFSATANQASGVFSFFGFGGKPQQQQQPSPAAEPRSQKDKSKSLQALRDASAASAAATGPSSPSAPPSPSPVSSAAGMFNSVWEQLSSRLGDLERKISNQASATERQSLVVKGLRVKVQAGEAEPEMLVKAERTLSRMERRLLDLDRDYWAAKQNAKGALAGNIPTENGSGGRRSSGSREQSAAGADAAARQSDSDPEASGSFWSRLLGSRSGSSMDGSTKEAAVQQAAQAQAASPPPPPQPPAQEPPLQRALSPLFRRPPPSPPPQQQNPVPSTAAAVSPPIGQQQKQPASPQSGYRSIFGFGGADTGKRTAQSSVDTPPAAVPAEAAPLPRATSPRSSSGGFLGGLFGRPRQQNERGQVPEVQPPQASAGGKGSVGRTAAAASATPPSTSSSGLPVPVPAAPTAASGGDALVDGASRVIRNSLSFTGLAAKIATDLATAFKADAERYLKELEIQEAAKRQQRTLDLALLALLREAQPPVRSTCTFEEAEERFAAEARWAALADGGRRRELFGKYLQTAKKVEEQAAAAADAAFRAALRRFNVTAASRWEDIRDALDEVDTAVAGVQDAARRQQLFLEVVAELQLQAALQAASLKAELQFTSMLTELRDPPVTSTSTWALVRKAVVADPRCMALPERRRRELFEALTARLFEEETARYEIAAAAAEVAELAEAAESLKPAIARAAAVTATPSPPSDHPTSKVADLQQRQQEQQLHLQTSAAPPHPAPNAAGSPPVRPSGGAMGDLGASVGPPVLPGPAASSSETLTSFPLEDDDLSAALAGMEAALESALKAVSSAPLPSSFNKVGNTGSSSTTGSSSITSSSIIGVLGAGTNNPVNFPPVQFGSTPVNGATRSTAPPSSLGGAGALPAAEGIRLEELRREQARLRAEYEVMAQRLREMEERLKGQAQLMSLAEVPSDEEEQLGQQQSPADAQAAAAISSSGGGRDRDGTVAPVRGQRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFI1</accession>
    <name>A0ABQ5SFI1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Senescence domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="15337" checksum="AA05B282EC3E3209" modified="2025-10-08" version="1" fragment="single">EVTLAPMEASKATLQAATGAATRLYETPLEAVGAVCNVVGDGTAAAATAPIQAFGAAVHGAVDAITSAVKVPVGLAAAAAAGTVAAGTEAIARGIEGLGDLAAGAVDLAAGATKSTLYGAAVTAKGDAGPSIQGVAALGLPDAQMGSEGVGSEDKPATEPEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFU5</accession>
    <name>A0ABQ5SFU5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SREBP regulating gene protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_012739</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SPRING family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019352">
        <property type="entry name" value="SPRING1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13481">
        <property type="entry name" value="SREBP REGULATING GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13481:SF0">
        <property type="entry name" value="SREBP REGULATING GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10218">
        <property type="entry name" value="SPRING1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045198312" description="SREBP regulating gene protein" evidence="9">
        <location>
            <begin position="25"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI68338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="272" mass="30162" checksum="C1950DF8E7EFEE01" modified="2025-10-08" version="1">MVPAVAGQRLLLLVFLAFIRIANAERRLLVRDLPDKGLKCRNTIQGLLLVADDRGVVCKRDDLNYATGCCSVGQQYDCNQCNMNDKCCSEYESCVSCCLAPVHDAVNVAKQVLRSPRHKESGVWGNAFDYCKGVCRTHSRSTAHENAYISARHHCFSQLGRPMLSDPLPPGALDGVEVVVGLKNEACDTFCLHRKKRCSQEHLRWLNSCDRLREHFGCEAGCEVVTGLGPSYVDGNAPKPERPAMCFVQPQEATTLSCSVREENHMMLCPCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFY6</accession>
    <name>A0ABQ5SFY6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013397</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008533">
        <property type="entry name" value="DUF815"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42935">
        <property type="entry name" value="SLR0930 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42935:SF1">
        <property type="entry name" value="SLR0930 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05673">
        <property type="entry name" value="DUF815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="53709" checksum="7D1BBE3DAC6833C0" modified="2025-10-08" version="1">MQHNMRHTVNNNPTNFHRHFFGSGCLATRKARVLTRGALQVKAIGFDFGDDDGRSSRAAPIKPSSKINNLGTLAASLLVYGHVVKGAVGEAYLQALTITQKYQASNKEILSAYARFYNLLLASGYDSWQDYILDQVLLGRDNAFARAAAQGTLDDDAPVLRAVAYDLDVLQELALPLPQLAELIAEAAPLGGAYWLEAASSVSIKSKKKGVAAAYAPASETPVTAIDPHSRPLYVGRPPTEAELAAWKAAISGKEAWSEAVQLLRQYYHLHGFGITSRNSTLRWTKGAFEEGADGSAAASLAPLPALESQRATLDANTLRHCEGSTAHHVLVAGPSGSGKSALLWDYTLAAGKDKGLRLVDVGAAEVGNILEAARGCGRYPRLRFILVADHVDLPMRGAADLMSGLAGSGPSGWPSNTLLYMGGSSSSAISYDPVVSRFGLILTTTELSEQDFSDTLRLVAGEDARLLRDDDVAYAAEWARKQMGTLSVRSAVHYVRRARSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGH0</accession>
    <name>A0ABQ5SGH0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013587</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF2">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="5">
        <location>
            <begin position="16"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="5">
        <location>
            <begin position="469"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="287"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="300"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="677" mass="71605" checksum="2DA3CFFE21E5F240" modified="2025-10-08" version="1" fragment="single">TETRTIDPKAYPKSMRHVVIGAGVAGVSCALELARCLQHEPHRDDEPRNQGSPAGVANAAFSTDQENVLLQPPGFAGTTSETDEIILVSSSSVIKGVREVARLSQLVEELQLVETPLKEWAAGAGGRLKVVRGAAAGLDLQDRVLQLEGGGALRYDKLCLCTGARPQELQVPGADHPAVMTLRDTDSVQELSHRLRSCRRLLLVGNGGIALELAGELLQLRSRAPLHHPHRNTPNTSAPMGPTMENTEVQPPELVWVLKHGHVGDAFFDLDAAHFLLHERLQSAAGQDAAQLDPAPPGSSPISTRTVNSDTAPAGIIGSGSRLEQQLEDAAGGRVGSGLRGHAAGPGWTQELLRGLVVAHDERVENEGGGTSGCASVSGGVELRRQRDPAQTATSSGGGVAGIAGRGFPNLRLVLEFSSTVSRIDTGGTNADAAAHGDCMEEARGTEGSSGTAVPASAPSPPGGAPWPLHVTLSSGKVYGADLVVCGIGVIPATEWLPPALTRAPDGGLAVDRYMRTSDPHVFAAGDCCTADWPDGSPTWFQMRLWTQARLMGTFAAHCMLGRVDEVASGFNFELFTHVTRFLGTKVVFLGLYNGQRLEHEPAKDIQLFSRVARAADGSGSTFVRVLMLRGRMQGAVLIGETELEEALENVILDGLDLSAYGPHILDEDFELDHVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGZ4</accession>
    <name>A0ABQ5SGZ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Plastocyanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_013586</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Plastid</location>
            <location evidence="2 10">Chloroplast thylakoid membrane</location>
            <topology evidence="2 10">Peripheral membrane protein</topology>
            <orientation evidence="2 10">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the plastocyanin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04219">
        <property type="entry name" value="Plastocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028871">
        <property type="entry name" value="BlueCu_1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001235">
        <property type="entry name" value="Copper_blue_Plastocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002387">
        <property type="entry name" value="Plastocyanin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02656">
        <property type="entry name" value="cyanin_plasto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34192">
        <property type="entry name" value="PLASTOCYANIN MAJOR ISOFORM, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34192:SF10">
        <property type="entry name" value="PLASTOCYANIN MAJOR ISOFORM, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00156">
        <property type="entry name" value="COPPERBLUE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00157">
        <property type="entry name" value="PLASTOCYANIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00196">
        <property type="entry name" value="COPPER_BLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0186">Copper</keyword>
    <keyword evidence="6 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Blue (type 1) copper" evidence="11">
        <location>
            <begin position="49"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="144" mass="14565" checksum="5BE102D4FECC23F1" modified="2025-10-08" version="1">MKATLRAPASRAGAVRPVASLKSAVSRVASVAGVSIASLAVTLSAHAVTVKLGADSGALAFEPSTVTVKAGETIDFVNNAGFPHNVVFDEDAVPAGVNADAISRGDLLNAPGETFSVKLVTPGEYGYYCEPHQGAGMVGKIIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHB5</accession>
    <name>A0ABQ5SHB5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TATA element modulatory factor 1 TATA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_013798</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022092">
        <property type="entry name" value="TMF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022091">
        <property type="entry name" value="TMF_TATA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47347">
        <property type="entry name" value="GOLGIN CANDIDATE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47347:SF2">
        <property type="entry name" value="GOLGIN CANDIDATE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12329">
        <property type="entry name" value="TMF_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12325">
        <property type="entry name" value="TMF_TATA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA element modulatory factor 1 TATA binding" evidence="6">
        <location>
            <begin position="1053"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="58"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="117"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="386"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="963"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1009"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1172"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="483"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="610"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="838"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1055"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="973"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1024"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1172"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1194" mass="124474" checksum="BE08AEBAEE1F2B34" modified="2025-10-08" version="1" fragment="single">MWGTFVPDLGKLGEQLTAVAGEALQKATADMEKSIDNTLGIRGGSKAAAGTVAVEAQVSSASGRSDGSAPLDPTTPNAAPGSKPGQKKVVKKIVRRVVKNSGTDLAAAGAAAVAGTSASAGANPPASEDGNAALGIRPAGGTVSSEDIHSQPTPGSSEQHTTAVENTRLTAASRLNGAFLPDPESDMPLPLGNASSPDVNVQPVFVSVATGLGNQSGESNHGAAPNGIFQVGNGSLEDAEMVGPVPQEHRRSSSGAAPGNHVVQTRELLGGEFLGGDAALCQHQRYEEQAAAAAVAATAEGAAEHTTTPAAGRDDWDAWQDEDSDMSVTRETGAAGFAGASGSSPALGPAYARIPEGALTTTVSEGDAAALGAVAAAGPKPEKTLVGVSRVSEPTASRESARCAEAGRGAPVEYGASDHESASAAVAGSKEAPDISPPPPPAAAVNGEVVVAGSGADNRAKEKADNAGAAVDAAADLPSSSAAAAASREVEDLRRALAQLQGELRERELQLQFQAVRVSDLQQVVAGLQTRNEELALRTAGLSEADFEAVRSEFEQRLAAAERKVYALTKERDALRKGAEKLADYGALVKEKDDIIKQVMDEGEKLSKKQVELESIIKRLRGQLTAVEAERDKLATRLAAEESGAAELRRVKAKLEKELVAAGEQAKADLESQREHFELLLQKAKSDQVDAEERARDAAAQGLGRRLRESEARCEALAESCAELRDALDRQRQAADLREEMLKADINDLERRCQAAELRHQDLAAKMPEATRPLLRQIEAMQSAMEAQAEAWTGAESSLHSRITDAEGRAAAAGERERLAMDKMQLLSARVSGLEASLSASRSEVRLMNEQLEEASQALAGAREAAAAAANQAEVLSERCRLQEQQLGQMETDIRELREAERTARAAVEHDTAALRREYERRLVEADMELGALRAQVQAEAMKRPEPPAMAAPGYRWVLVKDGEQQPQHQQQRNPNSSGAETSYRTSNAPSPAAITSANIGSELLASLRSGGGGGASTTSVAEPGGGGSGSGHHGGGGGGGVGTDSVQMVMASELESLRAALRAKTGELVAAQQQVVELEATRDRLAEELVAGSAHTVAAEDAMQQRDRLEAEVADLRTKLSSALELVGERDEQVEELLADLQDVKSMYKDQIEFMCQQLLELQGLQLGGAHGPGPGHSLGAPGGRALHGAQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHD2</accession>
    <name>A0ABQ5SHD2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013903</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="370"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="650"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="840"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="128"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="573"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="894"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1146"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1271"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1515"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1578"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2064"/>
            <end position="2158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2190"/>
            <end position="2381"/>
        </location>
    </feature>
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        <location>
            <begin position="2434"/>
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        </location>
    </feature>
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        <location>
            <begin position="2508"/>
            <end position="2751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2761"/>
            <end position="2780"/>
        </location>
    </feature>
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        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="128"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="220"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1154"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1271"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1329"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1387"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1530"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2067"/>
            <end position="2088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2143"/>
            <end position="2154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="2237"/>
            <end position="2246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="2256"/>
            <end position="2269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2336"/>
            <end position="2350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="2523"/>
            <end position="2536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="2569"/>
            <end position="2581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="2600"/>
            <end position="2623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2624"/>
            <end position="2638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="2660"/>
            <end position="2674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2675"/>
            <end position="2685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="2689"/>
            <end position="2699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2780" mass="292835" checksum="4B75F56DC0C78784" modified="2025-10-08" version="1">MFRRSNEHVGPGPRAPSNAPQLSGPSAASYPSWAPSPASASFQMLTPQTHPPAMAYPEPTGPRPAERTFLHGSSAITLNPASSSTTGPPYHPVPQHSPSYATYVGGPQMVPAMTAPLLGIAYPQHSAAMQSQQAPQQIPAPSQPHYQQLRPSHVQSAPQPAPLQPLQSMQPIGPMQPPSILQPQAMPAVVPQQLPLQVLTSPNQMRPPQPPMTLPPPVQPKQQRQPVIPVGQPRPLQPMQPLQPIQPLQPLQPMPPPLPPPNPLLVADLAAAAAAKGSSGGGPEAEPLPPPPPATAAQLLLPSASPMLREVVSEGRLYVLDTSSRLVYRDSDQAGVLERVGKWVQGAGVVFVPHLTEADFFSALRSFLSRRQLGLADLFAALDIDLDGALSAAELRALVTHVVPGAVPSDVAYLMALLDWDDDGYVSLADLLDADDLADQMALERAEGAASAWVLALQETSFILHSQRHHAQVVFRTLAVDVAAAAAGNPSALFHEEPPNHGRPPHQQEQRHEPQALLEPASLLRFLRVMRPELGPGEARYVMDHLLQGPGDNRRSLPQVLHTLHLADVAYSTDQQQLKQEPEGAMEEEIKDGSQQQQRDRRRQQQLQDTLMVGPTRPAPLGPSRLRRRGGWPREAVPRFFYHLGYYLRSCRLTLRELVDQFDSDGDGALDPGDVRRLLADVLPGATGAQTMYIRAAVSSPTAALITLQDLTRAVEQHDAEFTAGRDDATAAAAGFTPTSAFAAKYLQGAAAAAGTDGGVGGAAAAGAEAYLRQQQKYQHHQQQPADVLKWILELMARHKLQLATLLAQFDRDLDGVLDSHEASRLVLYAAAGRLLRQRPMDLALHRLLAEADMDHDGKLSYNDLRLALLSKQDSLAREHARQASEKVAAKAATATATELLGPSEPLMAPTLRRPPPSPPAHASSGGGDPDAMRPFWVAPEAAAAWPAGMAQYPGMRPPGTAVEEYDTTPYSPVHPTNPQDPFTLAFAARNPAASALPPSTLAVFPAPPLSPGHSGGDSWVGPMSLRRPFVFPGDIPLCDVEPLEVPLAPVEYLGIRVWVDPVSTLAYLPLPVQPRAPSSATSPEMGPLPLDQQEHQLHLFGRLLPNGTLERVETPLSAQLFAALDYRLRTAKARLEDIWTPAITGASPPSLPPSRNQRSQPGTHAAAAVAVPPADVAALARFLGPLLPGLTVTQVVYLAALFDLDGDGLVFPDDVVMAFEEIGTAINTSASKLNAQAYKLLARVAGAVLGDYAESYRAFVQYSNRMQRARQERKREKEERAKERKEKQKREKIKKDKKAEKGDKEKMCTDKGKEKKENNANKKEKDEKKKKKEKKEKKKRKEKTGKKEKDMQRKGIAGPPGSDDGSSAKTVSSEDGKKHHRRCSSRRNSSSSSSSSSSSESSTDASDEDISELEGVELTQPQLARFLSHLTNHSSSPSDVGALIVYLNLKLAAADGGASVPAGSPAAAAIAPGPNQPYGNPYGIPQPYRQLRDTEPMFDWAFLVGVLRGAETSFPVDRRHPSPPGSRRVTREREQAREKRQQQREAKALAKAAAKAAAEADARAAEMRAAEALRAVVEQETAEDEERSREGGRSSPLRVNALTLPPAPHPDRRLVDLRFHVHTDGRTFLLDVVTGLLYVASPYILAVSDAVAEAGGARPSPTVAMPYTHGGVGRTRLAGPPVLSPRLLGAGGVHVGRGVEVAGSYHASGGGSAQALPPAPPPPLLLPYPDLAGKLQYPDNRLLPAQSSSGVGLVCRALERLLAEEHRASALYKVLDRDGRGLDLRRLHQLISDAASLTFAEVAFAAVLLDRRGSGRVTLADLREAAQTVHYTAAALGASPSTAPPVTLTLARVGARALDVLHRAAQVLQREARLLWLLVTKAGAAGTGQLERATAESLLWEVLQPYLPPHEIRVATAYLAMMTPDKDGNVTADEVIQLLRAVPMCLRLPYSDPVAVAGAGGTTARAAGREIIFAAGFGGPVAADGTLPLIQPSVPGGGGNAVISPYNKKLRHPTGPTDVEPELLYGPNDGAVYPDVNMPSDPDWGDGPYGREADAMARMPPRRRMANPFGQLPQPDAAAQQRAQEAAGTYGLFDDTHPDLYDDPQPSSLLQGARRQKPQAPVSELPRPLPPGPTRISRRTGSSSAAAAAAATADPFSLQPQHESVAALGSTSVSMSLQEEADLLNKLRGRPPRRLQHGGNGGGTAAGYENSTQYGLLRDLDPPPALEGVSRTELRVGGGGSGAGERYGRDDLSPAPTPPLPRLPPGLPEMPFVGPSPRLPDMSPRPPERPVGPSRRMTQAGAAGGPVYDSSEALAERPSALRTSPAVGGGAPAQVLSFLDGDDSSSGVDANSIDDVSGRGSGGGSSRRRRAAAVSGSLHDASAVAATGFDPYGIHDDDLVNAGSVPSSRPMTAATLARNSTADMPRLASPALESLLGPGHFGGAQIGSEASAGDAAGGEDDDGAYDPYSSITQAPGKLVRRLSGRLDPALSDAGAVTSASGPALFTSFGDAFRDGSARPGSGSAGRGSDDGGLGGTPSSSLLPPPSFRTTHVGTPASARSRLAKSTTAEEDYDEYDDPYAEDNSAVGADLSALVSSPQGDGGGGSGGNSPGEGGSIAGGGSGMFNTSIRSQTATSPLGMSIRSGGAADRYSSPGRRTLTGGGGSGGGGGGTGTGNSSSASSNATRESAGGGGGDGGHGSDTYGLDMEGEDDMMGRDGGAGGRDTAVRKGTRGRDDAGSQGDDNDNASWGILQYSEADLAQGNEEGDFGLVGQNDDGDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIK6</accession>
    <name>A0ABQ5SIK6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013841</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad17/RAD24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004582">
        <property type="entry name" value="Checkpoint_prot_Rad17_Rad24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03215">
        <property type="entry name" value="Rad17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="711" mass="73330" checksum="DF4C6842B3121329" modified="2025-10-08" version="1" fragment="single">CGLAVLSGPSGCGKSACVKVLAEAMGFGVVEWMPPAPVIWNEHQYQHPTGSTDDGSGPPVSYQSKLDEFESFVSRSKYPPLALAASLTVQSASMRIDVGQVPVPGASQAIASAATRGSSAPASRPKLLLIEDLPHTHDAERRQRLVAALRDLAATARCPLVLVATEEGKAGSSGRSGPGVGGTLGSSKGLQKEILVALQSAGATTISFNPVTSNNIAKLLMRVAAAEGLELSPPAAVGLAEAADGDVRSALQALQMNAMLQKAALRVADAHVKDTKASRRGNGSRKRDGGGKKGGASMVTLLRVGRQQALTPGEVARLVAAQRDLGLPLFHALGKFLYNKRGGGGTLVAQGIDGDSNEDDDDVTTAAAASGGKDKKDSVALQQLNSYSLAAGRQWKLKHPVQLPARLLRPPMRYDPEAILLRCGLEAPVLLSFLHENYPSFVDRETQDAIDDISTVTAYLSDSAVLCGESRASATAATAGGGTSLWLEDSPAAANLSSAIAGSVATRGLMFGNTSPAPHCFNPIRAPASSAINQAAAANRLQLHAAACRLSVASGGAGGDAAAFGTSWDGSGTSDGNAGTSALFGGGSASQLVSTVMPWLRSLVQMSPQHATWLAPLLPSVWSYYWNGAVTEGAFLRHVASLPPPPYQQHHQWQQANSARWGGAGRAAGTAVSTFRCQPGVNGSGGCNSGDGAMESMLEQMGLEDEDPIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIP5</accession>
    <name>A0ABQ5SIP5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="217" mass="23826" checksum="28B5F280A7EEBEF6" modified="2025-10-08" version="1" fragment="single">QAASIRTDLPVPRDCPIYYYEITVLDDGEDGSIGLGFAAASVPLSRLPGWDERSYGYHGDDGNKFTSHGRGSRYGPRYGTTDVVGALWDRVRQVIEFFLNGESLGPAFTAVPGAQVLYPMIGLRTRGAKVSVNFGPQAPGGSPFRADVRGMERRARQVTMERIHSVRISRDNDPWDGDEAMQQSHRPSPGVTDSRGEITPNGATAAIPRITRRNHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ68</accession>
    <name>A0ABQ5SJ68_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="644"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="775"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="205"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="428"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="496"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="575"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="607"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="648"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="875" mass="84691" checksum="D32963E23738CD65" modified="2025-10-08" version="1">MRTGSTFELQIQGARTGIPSKDESVRFIGGSSAKRGAGVQLGSHDRSGPQQQVSPYLNSLSYGNHELIGGRRSGSSNIGTASSVGTGKSLAAGASAAVQEAQQRLERLLGPQQPATGVTGAVKRPISAPLTFGTPISASTNGAGSSVSGASNAAAPYSSASAAATGSTISSRPRPASASPLQTSATGTPVGSVSLLSTAPAAVAGSSSSAASNQRSASGRGLSTSESSTGMLASVGVTGVISSGHNSIAVSAGESVGAAKGNLRSPTGGLSNNKEALIVADQCILGQGGVLLDGSASMPALLPSSLESSSSSGSGVASGRRRSSGTPASALTTSSSEKVLGLSRLAIGSGGRSTGQSAIRSASPNSTSPKSGSGSGSGAQGTGLVSHGATKTQPQSRRRLSSGDTTGPGGSAGVLSASGVDGGGGRTGTATPTPTAGTGVTMLKVGQVVSASRPGSGTGGIGSQSGQANRSGGSQVSGDGLRVRTKTAGNDRQHMGPRSPASPSGTSAGANGAKAVSGRSSVIGTGGSSGKAATPGSGNKALSGRRASTGQQDTGGAATGLGGEFVLAGNPGVRPSSAAVGVGGPSSSTTSSGNGRPSSSERRRRSSSLGTTATTPGRSSAEAVAAAGSASGNGLSLGVLRDAAARQPSTSSPMSLTSMGGTTAQGSSGNRVKPPGIDLSALTSVSSAASGSETTPVLNRHAVEPCKGVAGVAGGRHRVAVEFSGSLETRLCPPVSPTMMGSPGGAGRYTGATMLDDSATPLPLLLLPTRSPLQSPRSALGLPRRARGSLGSGGALTSGGSGLPGPCRWARNSPFPQEAGGTALGSEMPASTVIMHDLQVYAHNLLLHVGAGSSTFIEALRERYVEAYRASRKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJJ0</accession>
    <name>A0ABQ5SJJ0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="433" mass="46578" checksum="F4291C5AE2A815DB" modified="2025-10-08" version="1" fragment="single">LSLGPPRAVHLTWHLSVKLRQVKKWMRMPDATASAARLSTGRDASAAATPTDMFGRKRRLYPDEDNDSASEANSSLLAMIDQQREQLTAPDGADDASNNNNVEKDYSSACAQDDANATGEAGQRKRQQREPERPRRRRRAGGRVTLDNLGEALVASRTYREWTLDSSVLLKSRINVAPDPSGGSDPGHNPGLVRASGTTATGPFVDPVNGRCFTSAPYDGTPGPMICGAVAGMLAAGHVAAASDAVRHGAAAMLNGSSMTWGRHPVAKATSCCRGRAGCRPGAAAVHGEERCPPCGHGFWSKGQRRAAAQGTTSTARDELLRRRKGRGRNRGSSRSSPEASSPRHAGRQRHAAAAPCSGAWTDGRRPLGRVPVKCCRQRGNPARPPRGAMRAAWAGRPKTRSYGTYHVQSTALRRSVCLEHARWRTCYRRLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJK1</accession>
    <name>A0ABQ5SJK1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="657"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="961"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1036"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1207"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1350"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="967"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1207"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1296"/>
            <end position="1311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1376" mass="144014" checksum="A845AC68A3379B4E" modified="2025-10-08" version="1">MPPKSLRRPTRKEAEVLSQNARFGYLSKASELLASEGYLKDAAYFGSRALKAADAQGAAVPRRIALQHCEVCGVFLSPSSCSSIECVDISNRRQRRAAAANATPAPVGPGNRSSGCKAASNVVAVVRTCQICGHRNSQPFAVGHVARQIAESEARAVEQLKLRQQKQQTRNSFLKQNQEVPTPQRPDGQMMEDLPKKPMEKLAQVVTNGAAAPAKAVETATVQTGCRATDDTIYVADVASTGVEGWDAGVDQVAGAFKAPAVHADEMDPAIQDNSAEALHAATAGKANHEGVMAPVEGLEALALPSLMDSVQTTAAEANGNPADGEVAAAQEVQVNPEEGMAVSMVADNTQEAVAAMGRIQRTGTTEDAADVADLGNGAEVFVPMSDLAAGIPGMEGQRVTEPQDVADCASNQVADAADESFDGMDGAAGGPTAAADGSSEAVPARKQPEEQGSAGCAAQDKMAAAQNAGQIGGLTTGALGHVIAESGVAMAPTAFSGTRKLLEATDPDVAAGATALGNAAGDHELGLQGAPENPCTAVEPAVGVASGCAITMGALASPIDEVAAGLPTAAMGQESLDVTCVLTVPGATPLAEQEPSGVTLVQTADAGEGLMDCLKGILQHAAQECVAAQPCTFLPGNMLERPGATSHMARPGAVLTSSRDENGEEGTRNAENQGENFEEEDVEALGKAMGVIAAVSGATGPITGTALAAMAATGKAMAAICCIDDSESSKHKESTPKEAVAGLLAEMRVVEAAMAQKSTPPFLHAWPAEGGKEISAVGIDAAAATATPPMTGEAANVAGGPDGSALTASARMIMGVTEELYAVESQEEKDDGGWGIPGITSGDVSQDGVLDKEEASGNMVVAASALHNADSGSLSQLSVERKPAALHKPVVATEAAMANAAKTAAADASTCAYEVHAAPSAVKQRCVARCESEPQPAAQGWARPAKPAPPLLQSQELLSKKHGKRTQVQTQQQRQVTTTRVLSARAGSEATDAEVGVAARRTGEEVPVLSECDVASRRAFILSEAPLEALMTRYQRSRRQEDQPNTQQSGAHHTGAEPSHKRRRAQPDWLPKGYICETSIHPLLPQERDDGALVYEAPYAQGLFDLRPVVELKWRRALHQPHYRRRFLRESALHMAHYARFVSEPLQPQPKERPQPTAAGMLRPGRLRQYPSAVAVISEGELTSRLSIDRSATDASAGMCLGTRQVRKERNGRRELQKDGKVASESEEESEMKNGIPRLGPWARLPAWEGPSGSDNMDHDQADDNEPLSGKALGGSNGRRMVREQSMQKPKHDSPAGPVAAAPESAAMSAIKTAETEDRRWKQLQQRNENRKQAAPVQQNKKRVTWAAADDGRQQQYPRKKHSYAPVFTASVSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJK4</accession>
    <name>A0ABQ5SJK4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014794</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="367"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="594"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="639"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="989"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="989" mass="109225" checksum="F97E52263CF558F3" modified="2025-10-08" version="1" fragment="single">MANGEELPIYISRARQLQSDLLDVGHAVADTELSTIVIKGLPSKYHVVTTALTMREGELDFTQVVARLMAYVIPDEDNNESSSAFITHRSGGKFNPTSRHQTSKQRSKSNAICHYCGKPGHFIGVCKKRLADQQQQNNGRGGGNLQRREPTKMAYAVGSESRSDAWHLDSASEWHITYDASELKDLRAVRPEEKFTLVGYDGSRHQPTHVGRYTMWSNTVKGLQIELKNVYVVPKATVKLISAGIFDERGATILLSNDKALVQAKGRTILRADKVGRLYAIRHHAAGRSVGKGRSCAAVGSEGSKAALWHRRLGHLGYKSLERMCREHMVTGMDLDRASLEAARSRICEPCIYAKQTRGPFPSTGHKAAKPLGLIHLDVCGPMPETSIGGSRYVTTLLDDCTGFSTVAFTDTKDAVRKKVKIMIEALENMTGRRVKEARTDRGTEFLNTVLGAYFGEKGIIHGTTVGYSPEQNGAAERLNRTLLVKTRAMLAESGLPQKLWAEAMATANLLRNVSPVVGASVTPHEAFMGVKPDISHLRVFGSTAYAHVPKEKRNKLQPVSKKGVLVGYEQGRQYRILINNLISTHSTVKFDESTVGDRYEDSDEEDVEESGGVPLPGGGGDTPVNTDMDQGAAEVNHGASTSSGQTAAPQRHAGIPAAQFPRMQNTAQSRGGNGGDAMTGEGPVQQQNHNNDGADVNMQERRYPLRERKRLIEWRHEPNGRVTFGWINAAIEEEVPEPASYQEATDGPYAEEWRSAMDEEISAQLTNGTWELAKPPPGARILPCRWVYKVKRAEDGEIERYKARLVAKGYEQRAGIDYGELFAPTSRFASLRMLLAVAAAKAMPIHQLDVSTAFLNGELEEELWMQQPPGYESADPEQACRLLRSIYGLKQAPRCWYMKLAAELDKLGFKPSVADPALFIKKDEKEIVYLLVHVDDIIITSEDEAMIRRVKTEIGQAFKIRDLGEAKVFLGMEISRGAHGEVKLSQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJW6</accession>
    <name>A0ABQ5SJW6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_014835</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0229">DNA integration</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="12">
        <location>
            <begin position="107"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="350"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="406"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI70064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="604" mass="66244" checksum="F5C081FCB3C717DC" modified="2025-10-08" version="1">MGKDKLAEPLDLNNYSTWAYRFENLCAEHNLKRALLTEPVTDVEVAASERVKTLLVKNVKDCHLPVIKASPNTKMAWDGLAAIFSANNHARRSQLRQEYTKLAMKKGEALPVYLSRVRELHTDLLKVGQAISEQDMAYAMLTGLPQKYEVMVSVLTTQSAFTPTKEAVKDKVVTMVNQLETYSGFRVKEVRSDRGGGEFINSPLQEFFRTKGIRHGPTVGYAPEQNGAAERLNRTLLEKMRALLAESQLPQCMWAEAVSTANYLQNVSPVSSLPCTPYEAFLGEQPDVSHLRVFGCTAYVHIPKEKRNKLDPVSRKGVLVGYGDAGQYRVMFPNHVVSMHTDVTFDERNVGLQSPRQAGRVQKTVRWQNPVAGNWDSDSDDEDDCEPAPQTQGGTSPSPGAGGSGDNSNGPAGGANTSGAGPSVSSPVAGPSVGAADAREGFASRYPVRDRKRPSEWWRNEEPRSVTGRVHVAAATGESEPKTYAEAMSGAQASEWKKAMDDEISSQLANGTWTLEKTPLGTKLIPCRWVYKVKRNQDGSVERFKARLVAKGFVQREGVDYGELFAPTSRGSSFRALLAVAAARKMPIHQLDVHHCSGILPLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK26</accession>
    <name>A0ABQ5SK26_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="1">
        <location>
            <begin position="161"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="326" mass="36003" checksum="3FC28264590605C7" modified="2025-10-08" version="1" fragment="single">PLPRPDDACTQCIKIRFEVTNPLSDFKVSNQTCNLILQHLAIKFNSIVQHFNLSKTITTTTISSTSSTTTTSANFSVDPENCYPTSINICGEFGNWTSVDSTTRALLSDQFYFVTDNLIGQLIGYLCRGTMGLSIYSVSLPENCFDVEVSRTCRPPALPPPPCKCNQTIGIMPYYTLPTVRTESGRKNGATLYCFSIAKVQPFDPRASSCGSSDLLDKIEIYANDTLRRKINGIRLTPNNNNGNGTSRWIYPNWGAVGTNWLKITPLRWDSSQATGGQFCLELTIPLDTFCARDGTCSVSLFNEDRDCCPVYPAALPYVPVFDLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK50</accession>
    <name>A0ABQ5SK50_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SDA1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027312">
        <property type="entry name" value="Sda1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012977">
        <property type="entry name" value="SDA1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730">
        <property type="entry name" value="HSDA/SDA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730:SF0">
        <property type="entry name" value="PROTEIN SDA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08158">
        <property type="entry name" value="SDA1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SDA1 N-terminal" evidence="2">
        <location>
            <begin position="56"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="849"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="627"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="719"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="886"/>
            <end position="909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="909" mass="100252" checksum="5272905880A68DF2" modified="2025-10-08" version="1">MSTSAELLALQGNIKKDPDGYKDEFMLQYRHYQALLEIFKMRPSKESKEFGDLVMFMAQISRAYPKTMTCFAGELSSLLDTHQAVLDPALRRTLVGALILMRNRGQVEAMSVLPLFFRLFRCQDKALRQQLFRHIVADLKAANRKTRNERLNRAVQNFMYTVVSDENEAAAKRGLAVLTEMWRRHVWRDARTVNVIASAAFHKSPRVLLAVMKFFLGQDKDSEEEGSDADSADEDGEGKKPVGPSKEDFYAAYHKGTRSSKKKKQAKLKRVLATVKKAARKGGAAGSGVESFAAIHLLHDPQTFAEKIFGRLAKGAERFETRLAVMAVLSRVIGVHKLLVLNFYPYLQKYIAPHQRDVTAVLAALVQAAHDLVPPESLAPVMRQLVDQFVHDKARPEVMTVGLKAVRELVLRCPLIMNEDLLGDLAQYKKFREKQVAAAARGLITAFRDINPEMLAKRDRGRAADLALKPREYGDLGVATRVDGAELLEKALAEGRTGSDDDEVDVGWSSDEEEEEEEGEAEGEEGEDSDLESLSGDEEGPDDSGSGEWEEVSHLDEEEEEEEGEGSLKAVVRVARDEQITPKPNTKAKTKTKAEGCVTGVAPKPNDDRFRGGRTVPGAKKRKSEDTSEEDEEEGGEGEEGSSDLGEGEDEDEDEGEEEEEEEGEETNAGRGRRRQAGTEQRRKGASADPDSLTSLKRRLAEAKAAAAAKKKRQRRQSDGGGGSEEPSSGGGDGDVEGDESRPSVTPGVPIEAERILTQEDFNRIKKLKQKAMVEAALAKHGLASGVSKTKRARLLEAAEEEAAEALALRERMAAIGESKVHADDLVGRHKARKDKAARLASVLEGREGREKFGARSSLKKKKTAGLSEREKQRRKAMPMAARVAQIRRRNAAARGRGRGKNFKGHVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKF2</accession>
    <name>A0ABQ5SKF2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014564</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="8"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="388"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="501" mass="53932" checksum="D32328788A7D4D53" modified="2025-10-08" version="1">MSALATLREPVRTIALPPNVSAAAILGQRGKLVNRVRQLTGANVRYIPEDHRVDINGSPRDVGAAARLLERTLEHYQYSGLQFTRKQINNILSPELSEASALQLQPCSRPVSTVLGPVDGFVLCPLDDDGSSSSSGGKGSPALNSGGTAGRQQLGASTSTSSTSTAASSAPLLQLAGDTDACLQQLIDLAAEISLQHPAECDRIEIRLYLGKLVLTDLASSARVLTPEVMSGWTFQRNARTVFETSLPQETIRSLDRYLTSKGLTATHQSKSASIHLESSISWIQYHPTFTIDSGGELRLSKLETLGCKPMTVALVGPPNRTDLRLRYVASVQHNDDDPVAAELRRRQSEFALVNNVQVVVPNDLHAMDLTLTSARLKLKRVYTSMTPLPPPASTSSSSSASSNAPSGIRRPRASKRPTSPSLDSEDLPPVRLKVSVAAVVDNLGGKRLEVTAICPELNATLMEIHRTRQEAPGSRLILAAQLRSLLSHMADLRANVEFEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKU1</accession>
    <name>A0ABQ5SKU1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015537</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15241:SF304">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15241">
        <property type="entry name" value="TRANSFORMER-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="7"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="89"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="339"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="483"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="521"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="570"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="601" mass="63741" checksum="C68320E488183514" modified="2025-10-08" version="1">MSLWGCEELYVTGLPADVTEPELRGLFGTHGTVKEAVIVNKKGPSGPNAPQSCIVRMAAHPDAVKAKKALHKYDWMGKQISVKWSHNQRVLWVTNLHESVTNEVLQSAFAQFGAVSKAIVACDAPNNTSKGWGFVAFENKRFAVKAIEVVRQRPFLVGAGMKPVVADWARNEEILEGFSEEVRSGHKGPPPGPLLLENAARFPAPHSKEDMQGGRLLALRAEYEDLRRILKSRLAEAENQILVTGNAPRLSHMLMSDPYLAPPPPYMQAPPPRPPMGPPPPPLHGQIGGPPPPMGSPGPMGPPPPYGYPPMGPSGGPSGMKREGESFHHVPPPSKRAMTGNQTERFNPNPNAQPKPDQLQQPGGQRSGGSFVRGGELQTVNQGGEAAVPQHVHSEGSTAPPAAGAKQPGSHDHGGIGFSGPPAGSGGAQGKAPTSTGASTGQAPYTTYQYPYTTQQQTGQSSNATPPSQGGGSYGAGAPPPFQQQQQYAYGAQTPSGPGPAPQQAPYQQPPPNTYQPPPEQQQYQYSSYNGYNYYQQGSAAPQQPQQQPGQTHSYPGGPQGAGAPGGPPGQQMQQQSYGAPQSQHPMFAPPPQGPQQQYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKU4</accession>
    <name>A0ABQ5SKU4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014888</name>
        <name evidence="2" type="ORF">VaNZ11_014892</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="1 3" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="230" mass="23711" checksum="9480751BBA0EBA66" modified="2025-10-08" version="1" fragment="single">IPMKMIPLEAFCYDAYGLYDHRPWTPAETEDVGLAVASTARGCNEPGPIKTVKTSASMEAVHDPCTSTQLADVQHRNGPLSRKRRFEEMAAEQQGLAGREGVVAAEVAVATEVAVVAVDGAAGAVGDAAAVAAGVETGVEAGLDAEAEEGQGEERATRQRVANAPDGKPVAVFRGQRPRPRAASVMKVWACGIMAWAVPVVAAAAVATGGFGLGEAVCEVLLAEGMAGWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKU8</accession>
    <name>A0ABQ5SKU8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015515</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050769">
        <property type="entry name" value="NAT_camello-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947">
        <property type="entry name" value="GNAT FAMILY N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947:SF37">
        <property type="entry name" value="LD18367P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="351" mass="36624" checksum="79BA5B8B8F374356" modified="2025-10-08" version="1">MSVKVVTLPSGTVIRIRPYRTDDHDEVIRIFSSGMMGLVPSGVRQILQTSRPLQVFACAAPAATLIASPKRSSFLFKAAASLGAAAFVPCLVFALIRRTVKRYIFQSVDGPDLSNIETFYSTSGAGHGSAFWVAEIVEQGPVVTATPDTGLTVQQPPVVPRPASTASSQTSSGTANGEISNNLLTSSEPVTNPPAVPARLGAVERETWSKAQECQSISTNEQAGVITAEGRSSSTVLLPAEPDERQIREPAAGLVAGAVARLEAASSRSLTTSPRSIGPPAAALKAPLIWVNADDNPGTENQPCENTDLDSRVGPKSVPLPSVVAVPATPAGGAFSSSSRSFIAQRRGQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL08</accession>
    <name>A0ABQ5SL08_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014878</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="537"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="68"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="565"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="593"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="609"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="629"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="642"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="666"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="701"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="737" mass="81012" checksum="C520E197F4A16C42" modified="2025-10-08" version="1">MDRDEVEGVKWDAWNRALASVAGILAVGGRRDDGALDQLYNIAVELRERLRYSQGRLRGLKQELSVTVAATRSERHEAMARLQELETEVARLAGAVQAQGTTLAARETVSQSQAAENDQLRRHIAALERQLQRSEEDRRVTMVERNDALRHLERMTKLLEESEGRVAEVQGEHNRLLGELVVTKGRLADMSALETLYGAARDELQRLDKENLELKDMVAAGAVQLGRSRILEALGIPDKPATERSTATPRTRRSRPGTPGATTAIMSYGGSPGSVGLAAGGLRLGSDRSRSAPRSRRGTSLPSGSISGPPSPAGRLQVSSPRAASRAHSLSHFQSNSQSPSLEDPVSEMAFLGPPSAAYPPDPENYLFYRVGGGYEPKVNPTQPTNPQPRASGWVPKDVVRLIQAFRHSYGLSLEWRFWEPLVLMIDEVYRNRADSTLAAMKAAQQEKLKAIKRQVRAALGYPSAVANGKINHLQRQLKSARKILAWSDRKGEQSLRQMAMKDYHLGYQLDRAVEENYTLRRSLQDLEQRYSHTIEGAAPGRFSPGGHVTTPAGSGVPRGRSITSASPPSAAGVPSPQRSNSDLYASALHSPQRMSSIASQQHIMSPKRPHEHELERFHRVSDRYVGGPPYPYPHAQPQPQPQLQTQRSRQSLNQLPHEADDGLPSQRSLSQQRNSQYSDQPQQFPRSHSDSQGHSHSHNQLRASDGTFSRRTSMSALPGGLGARTDGASMGGLSHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL82</accession>
    <name>A0ABQ5SL82_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015782</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1603"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="707"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="796"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="970"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1043"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1199"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1243"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1353"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1422"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1917"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="970"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1067"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1147"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1170"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1201"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1245"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1284"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1298"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1353"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1426"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1442"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1926"/>
            <end position="1937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2131" mass="222379" checksum="95BB13B7D4BF9F4A" modified="2025-10-08" version="1">MPRPAVHSMSMPLEERYRREESSTQHYSEGACKSDPSSRASGIRGFSHCNRTLRSYINGKLSGPQLLRPTGRYFLCKAVSACFRRMFLYSTFLFLVLSIPALFAPVTDAATDASATEEPPVVAYCNTAASGSCPYCKGDQRSPCYLATTTDSWYTHRSWCILESPVGACGKPYTYDMIAQNNKSNIGGQVFMFQDVKNRLFITFKLYCPYTIRPGSMVIDDDVDEKYSLGVYVWNTTRQASRYQYSDGVSQYSGTFSCLTAVIDLNNVCDPSCSQFSNDPFAQLNKMCSPDLTATSCPPGNVSTHRDFVVQPYFNAFTTFGSQCRISVNTTTFILNGTENSNYVRWDAQACGPKGTPLAAPPPVPPSPPAPPAPLPPQLPTPPPPPPLPPSPPKPRSPSRPPPTSKTRSGTEPSTPPTPLPNLPPPRPMFPSSPPRLSSPPPISMPPSPKPPPPSPQPILMPPPPKPRPSSPPPIPMPPSPRPPPLPRPPPSSPAPTSSRPYMPPPLSPRVIISNPLPLTTTPPTGNPISPAKVPPSPLHPGETGEHLVPAAVVGSLSPSPPKEPSPPPPAPHQPAPPGHGNAVKDFFSSTGIGVLVGCSVVAAALICCCTVTIYIFCRRSTSDQMSVSGEQALSVHLSNDGGVNTRDDQSLSNFSFGMAAHGSEKDRSPSSSDAASFSAGIGYGDVERGSGSRTCGSNLASPGWGSMSGASDLQRAPTFQSELPSSSPSSQVYMKLEQGGNRPASSTSSDLLAMHAGQAAALRTQAQNIAAVGGAGSKSTAPVVSWPVLAPLRTSDRKASASPNAVGAKPQPQQVSPQFVAPITPDEPRPASGGGSFLKFAKRRFLPARKAPSTSEIPLAACQSVDFVPGATLGGGTQNASFGSATDEASPDDAVAAAAAINAVATLGAQVALGAGAYGGASKQLPSQPSAQQPPLISRLSSALRLGGSASVGAVAPYAGQEGLDSLSYRSRSASTSTAPGAPDPYGTGPITGAVAESPDGTSGSGYYPSVIAVPPAVNPAATLVTTKSVRRNDAGANLYPPLFSKQPSATKNNALLPNATGGTALASTQPVNRAPSQLPTIPSRSPLILPESTFATHAPKDLDDDDDGGRPSAGSLAPYGSALAGPTAPWAPPMSRPSELRPPDRTTSSGAHSEVTLSSPSLKAFNDSGSSGLSSPSPSALPAAALAAAQQPLHLPSASSLRYGTEGTRSSSPVGLQGRSSRENAFVDVSLPMLASSSFAAAPGTSGPSVSTSSGGSPWARTPLSSSVVPGQADSKFSFRSGGSGSNGGPDGGAAGSSFHSGSSRGPLTLSGCGGTPGLPPTSSPLPSSSPFQPPPLVAIVQPEVTAFVGSTSPSKLRSSGSIMQTPTPTLAPPAPLHLQPLDGPNGSLIRPPQPLAQQQRNSLPTLNLTSSLVNVDGDIASPTLDSRSSSPTPLQQACRHNSSNLSSPLSRSPSTSPLRHASSAASPLPSASCSPSLSRQPSRRAMVGMTSCPNSPPTGAGGSQRILSAGSLTRLDGRPSLTSTASAAWGSSFSHFVDEDSIRRYCGNGAGGDGGLLAAACGCNPSGEEVPLGGADAAGLTAVYNAVCKLEQGALFFNKYKIGGSPVMGRHSVVLLAKNVYSKARVAIKFHADYGQYQREKDALQALSSPYVPALHEPLDVPQLAALGLPPALVMEAGRCTLTEWLRGGPDGATRDLLSLKGALHQILAALMHMHSHRIVHRDIKPANIMWFDDAHRFKLIDLAESAAIGEVAPPCCTPLYSAPEQIKAALDGKACQAAVTPAADMWSFGIMAFEVFSGGLRFYGPSPTLEDVLLAVFGHQPLPSEGDGAMAGFDLVQARRLISNLVVRNPAQRWSAATCHKNALFASGDDTAQRATKWDSMLAHIATTTAEIRSAQMAINFVICKTTDPSPAGSPGLDRLMSSASASTTSSPPKLRHMDSSRVSRRCAYKEVPMIADDCLRGEPIFMLLLQKQYQIRITVAHAKGLQLSVSNIEQLKLTSPDNQVQVLPVRLETGLRDGTVEYVADWDPAASRSDHLRKVTPYEGAIQRRVVFLNAELVVQLSGVAQPTTVNTMLYVVMNDKQSARYALEKLKLWSTERWNQAPQWMRDVAKGTMVLVRLAGNVVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLH0</accession>
    <name>A0ABQ5SLH0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="308" mass="34864" checksum="22A6A7AE0A2A8AEE" modified="2025-10-08" version="1" fragment="single">MQARRTNSCRALLCVTQPNTLLLPFAVPLLPIHLLVLLLLPLVSLTTFSLGTYAAATPTTHDSLAPAPSSTVVHSLNRRTSALPPKRANLTACPNLRIAIFNGVPYHYEVLAGLAHLFRRYRHRTDVYVHRFTQREAGEGSWDALRRFRIKYHLLTRTRFEALARDKPFYDVVILVSPEYELPELENLLPNIRRHLTVTIVHNSDFEDMQRLIRVAEDGCSSMQLVTLSPHTAASLAARVGRNVKWALPVYPFKPDMDCLSASGMHSLHTCLRGFSIQGRFSSQRRNYSTLWQQIASRRSELTAGRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM17</accession>
    <name>A0ABQ5SM17_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000092">
        <property type="protein sequence ID" value="GLI70191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="194" mass="20671" checksum="9CFF01892E173609" modified="2025-10-08" version="1" fragment="single">SQLEKLRSGAAMMEARKGGLSGASTDLQGSSITAKFAIIPAASCPAMGATSPPSAVEVADAGAASIFINKAAQQPQELYQPLELTQPPKPHPKTPERRLPPEARNWGLQDCRSWAGEQHWSSWAEVQAFTKTLEPTLAEAIREASLEHNPLVAFGKEQAAAPAISYVQLKLMELCGSNRWGTASIKAASLEGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMQ7</accession>
    <name>A0ABQ5SMQ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2415 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015944</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019417">
        <property type="entry name" value="DUF2415"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF9">
        <property type="entry name" value="DUF2415 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10313">
        <property type="entry name" value="DUF2415"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="199"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2415" evidence="4">
        <location>
            <begin position="237"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="323" mass="35349" checksum="5E72CB0B1EA9ADFA" modified="2025-10-08" version="1">MGSLAEPAFTTSQAQHDLTTTIHHWQLRDLVAAGDTSDELLYVCDSSVFQYNSRTSEVTAVLSLDWTPNSMTYAHGMLAAGGPSSQLCLKDLREKRVLHRDPLGGSVNNAVHIAKMAGQLLLYACNNDHFVKVFNIGPQGLTPRTSFRTQVPINYCALSPDERFLSCVGDCAETIVYAARESSYSRLHSFREAHDTGSSTAWSPSGTYLASAHQDGTLAVWDIRSLSLVHRYQLTTACRNVKFSPGVMDLMAFAEHEDTVHIVDVRQWANVQLLNAGCGQGRDISGISFAGAADRLWVGLDDCVMSYDLDTVGRRAFGYGSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN62</accession>
    <name>A0ABQ5SN62_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone deacetylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015489</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF44">
        <property type="entry name" value="HISTONE DEACETYLASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="2">
        <location>
            <begin position="484"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="857"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="937"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="857"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="882"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="965"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="980"/>
            <end position="990"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1060" mass="108624" checksum="00559EB65D8399A9" modified="2025-10-08" version="1">MDSSGTKASGAIAAVQQAAATGGPAAGTAPASATAAAGTNGSARAGSNALPAAPRAAPAQQDQHQNYPTPRTRIYYNPELSKFSLGEDHPMVPHRLHLTHRLAELWGLFEDPAVKVVREYPPATTDQLRRFHTDEYIAFLEYLDTLDLANMSVEEQQELLGEDLALFGLSVCRPSVLPGAEDGSATALVQQPAPLSRGTAAAAPPRGAAATGPASRPPQQQQAQRLPTQQPQRPKPEPLTQSGNVGRQQLPASATAAQQQVVKMEPKVEPKPSPTIEDVHVPPQLIALAMQLLTKLAPTGNTTLTPAQHQQLITYLRQHQLQMQQKLAAAAPAGTTAAAARGGAGGGAGAAGVRPALTMVRPGQVVPPGKLGQPGPSGGGGGSGSKKRKAESSAGGGKGGKGGGGGGSGNNKKRSKKKRRTSYSEDDDEEEEISDEDDDPPTGATQAADGVAVGAGAAAAGAHSRPSRQAATAVAALTNAVRRTEAEESDDDDSDEDDDEDDDDEGGDCPIFPGLLRYVGLQAAGSILAARDLASGLCDVAVHWGGGMHHGMPYRAFGFCYVNDLVLAILELMSGCGRVLYIDIDVHHGDGVETAFKRSEKVLSISLHKWDDGRLQATNDTLNHGKPQRPVFFPGTGKKNDLGEGQGKYFTVNVPLQDDITDDSYFHVYKAVVKTAFERFKPQAVVLQCGCDSVAGDKLGRFNLSIRGHARCVELVRDLCRTGGVEGGPPPPANPARGGSQWRVPLLVTGGGGYTPPLVARCWALETAVLLGRQLGEVMPPAVAKAEDLSDLGPSYFKEPGLSRPPTCNYHMCLDTIMLPHMHKQMDSDYSLSRLAAYLTSNMQRINIRDGIPASGDAATAGDDGAAADGQATAKDGDTGKDGATGAATGSSKSKSRKSAVASGPGVADPLKHLTAPTGYPDAEEYIQDQKDRVVGGAPWAAKMPPPPVVRGRKSGAAAGGSGSRSGAAAPGGKNAPAGPAGGGKGGGVSAAGAAVIKAATGGGEAAAAGGATHGTTAAAFVPVQVPGIPVIRPYKPKVATPGAAPAVAAAPAPTAAAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ10</accession>
    <name>A0ABQ5SQ10_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase C19 ubiquitin carboxyl-terminal hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016987</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C19 ubiquitin carboxyl-terminal hydrolase" evidence="3">
        <location>
            <begin position="139"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="238" mass="25845" checksum="D58EAA6D5E020F74" modified="2025-10-08" version="1">MEDDPDHEDSPPRKRRRVISSFGKETNSFDQEAADGGAVGAIAQTVLPSPEKVAVPWLNVVRPGKLLGGGGPARAVSEVGDADLDLAEAIRRSLLSERGVVGPVVRAETDLDPDLEDAIRRSLEDDDGTEPDEEDEAVEILCEDEEMENDDHDDSGSRASGSTPLGQAGNYHLHGIVRHRGLTPLEGHYTVDLQIGNQVWCHHDDARASRVDLETVLQKAGEEGHIFFYKHAANFASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ82</accession>
    <name>A0ABQ5SQ82_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="678" mass="74296" checksum="C1A114F7613E5397" modified="2025-10-08" version="1">MPTGPSRRQPVPISGRDSDVIRGQFPEPGRYPKGRGGVYDSPERELRSGTTSRTQVPPISGSYHHAADGRGDIAGLRGDPYDRPLRSQFEAAQSRLRQDKASGALNEVAGRYRVLYGSHQLVRKGLRRSIADAYAAYVGQVVNLPEDTYGMALRALDRTFNTGGLPVGAIDQNIHTSPYTTSRVKRAQIFNTAHKIMRDLEGYVYDVSYYCPSELDQLQDMRRPASAAGESYKSRLRLGPRNTHLEQEAVGGTPRRPSSIPHSGATKRAKDGRVPVGHPIPSVNPITAKMRDPDPDLDPDLDSTLRPRERFPAPTKRRPLIPRPQTAPSTHATAAGGGRDEAAAAVAAEVAIARDVATTLNTAAEAEAAVRRRRGGEGPRQLDSSEDRIQRSWRRRGSSGMFRKTPPHYRVSKYYDHVSPTQEVIGLIRRRMLLAAEERAVKAAEASGGGADGGVRGSGGGGGLRGPQAGAQAASEATEKSTTVSGGRNLRATSRGGSPSAFARPLRPREDIYGRREGPQQQDTDPADTERIRWIIETAYELGLADVLGLEGPLPYARSERVYPLDHRRVFFEGLYSRPERYESYVPYAPHIYGDEMSPEDMAWTYDTPHRHLRSATAADRGVGGIGGGNATAAAEGATSWSYEYDDLTVDRLQTAAARRFIRQMKQDMYPRSRRLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQU1</accession>
    <name>A0ABQ5SQU1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_016692</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716:SF62">
        <property type="entry name" value="TRANSPORT PROTEIN YDBI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="346" mass="37400" checksum="90B7C82C27DCCABB" modified="2025-10-08" version="1" fragment="single">MRRLACKGGCPLLVHSRPSIFASTYARPPVVTVTVQKRRTCRQACRLRCTSSPTASASPDQLTATSSTPLAPTELQSLINAIPFKKLAIWGTVATLAYQLHDFAGMVLGTFIVTFIGNSFVEGAHEAPLVRSLLPKSTVRRQVLVLLYFLMILGVFTLLGVLTIPDIAREGADFVNRLQSDNIWVILVEKMRHGVGDQVMNSLEKAVYLATSNDIAVAAVSEPQVWSAERSTQLGLAVSSMLKGYTNTAAKYTAVFLKSVAKFSLQALVSLILGFLFVWDMPTISRGISTLRHSRLAPVFNEVAPVLTVFGQLFGKALQVQAQIAVVNTALTAAGMWLLAIPGIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLM2</accession>
    <name>A0ABQ5RLM2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000002">
        <property type="protein sequence ID" value="GLI58391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="156" mass="17630" checksum="741E95691EF415D9" modified="2025-10-08" version="1" fragment="single">QVDVKLQNVAQQQDGKLLEIMRQFTRQQDEKMRQFTQQQDEKMQDFTRSRMRKCGSSWCSRTRRCKTSHGSCWMRRCGSSCSKLRLFGRNQVARSYNVFAIDADHTLEPLLVESVQAQHALGTAPSAVPVNFPATVDAMSKLTNHGSGSVAGLLQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNF4</accession>
    <name>A0ABQ5RNF4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="112" mass="11007" checksum="1FF95F20263D9B60" modified="2025-10-08" version="1" fragment="single">MGGVRNRDAKMKANKVFSSGGGGGTKGPAVVDKRGQEVKCPYCDKVYQQSGRLKDHIAKQHAEQLRPTAEASAAGDGGDSAAAGASGDGGASSSAAHAPNVVAAAAAVARSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNY9</accession>
    <name>A0ABQ5RNY9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="148" mass="16184" checksum="90957480BD25728C" modified="2025-10-08" version="1">MAGRMTLYDIPVSINGARNRYIIRKKGLESEMDIVRLQTIGGPKSPQYLALNSQVKMPLLIREDGTPLPGRGVRRPGACCPAAHLSILAKGGKVISQYLMDKFASRGESLVAATPEARVKEIRTDKGTVNMDGLKVSIHAILLHSEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP04</accession>
    <name>A0ABQ5RP04_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025640">
        <property type="entry name" value="GYF_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037471">
        <property type="entry name" value="TIC56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37755">
        <property type="entry name" value="PROTEIN TIC 56, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37755:SF1">
        <property type="entry name" value="PROTEIN TIC 56, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14237">
        <property type="entry name" value="GYF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="150"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="249" mass="29046" checksum="921F446AD3DEE787" modified="2025-10-08" version="1">MSEQAESPSNSSQIVTRKIRLHKVMRPLEVDGTGRSQADFNEHIERRIAEILPSIADLPVILKNTIPSLPSDGRALELRRLQNTQQEVAFSEAQELARLFDMSKPHPLDNAPPPTPWAGALRPVPRKIVLSPYQYEMINYQRMLMRKNIWYYRDRMNVPRGPCPLHVLKEAWVTGIVDENTLMWGHGLYDWLPAKNIKLLIPMIRTPEVRLATWIKRTFSLKPSLNRIREQRKEHRDPEDHSLQVELMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQD2</accession>
    <name>A0ABQ5RQD2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRAM" evidence="2">
        <location>
            <begin position="250"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="372" mass="39715" checksum="CE2F573B4898FFD7" modified="2025-10-08" version="1">MQAGISHLRPPSSSNSSIEHVELMATSSGTEVGVEVEEEVEYVEEVVEYEDEEVEEDEEAHNVGTSKEQEQVPVCGAVDATVKSESRPSAEKAPNTDDQKQEEDEIAPEIPPSSSAQELSVDAAKSAPAQLGGTQTIDSDDVIFVQPSDAPSNTTGAGVDLDFKQLEAQAAKVANQVSHLALDVSSKLTAGLRSAFGGAEQASTGAGASLTKLAGGLTSWWGALDPAPQPQRDETAELVASSNKASGELQQLFGLSPEENLVEHFKCKLLQTYSCNHNSHTPAIQLAFQGTLYVTDRHTCYCVEERGRKAPFKVPHSLVVKATRQRPARKGDLSDVLKLELSNGQWLAFKDFDSGSALDSALALVEHLTESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR30</accession>
    <name>A0ABQ5RR30_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001459</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032854">
        <property type="entry name" value="ALKBH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212:SF4">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="484"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="593" mass="66275" checksum="1637C29119E0E90E" modified="2025-10-08" version="1">MKGICKGRTAGCRERPAVATRRRHAVSYSSLLNAAEVWLAPSSSHPLTVRAIWVASAVEPQTQPLTPRQRRLAQQRRKWEEERQQQQQKWDRPLLNQQYQLQREHQDACHPQTSPPLLKPTGFTQGPEISGGNPMTAPFPPADQVAQILDGGGPTRAVTTRSGTGRGVHAMSTPGRSAATASHASARRQSVPSPPQSPPQKQKQRQRQRLLPPHSQQQQQQQRQHQHQHQQEQQLAPSEQLHGASGWPRLFSGGEGQPLRADLDLYSLPVSQLISLAHQWANEIAGLEVPQAPHPHTRTSSARALLIRDITNSGTPTSKAPSLPAQAARSEGQPSIGGPPSQSQLPVEAARQLALLTAAVDAHVTRGSLGHQELADLTWCIGHYDRQHSLRVLHPAMRVPFSVVPRFMPTLRLEDFVSEIELRRDTIYLDDGSRAVEESRMTGWQSEIGATFRYSGKEMQAQAGGLSGQILKVRDELWALTGVKYDSVLINYYADGKCGMRYHVDPLYDRWTPNSAVLSLGDTRTFIFREVHDHNTRWQYRVRNGDVVLMFDDCQDRLQHCVKVEKRAEDAGPRMSLVFKERLRGPTGQYLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR70</accession>
    <name>A0ABQ5RR70_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001888</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="286" mass="30115" checksum="868B6FE8CD6F4BF3" modified="2025-10-08" version="1" fragment="single">QDITTTSTAPAPAPSSSRQQVLPTTDSAAGLTNPSCTETAASPPEVVPPPVVQTSRTSGLASSSPPCNQQQKRLAVQITPAVQARQRTTSNISKPPNRPAVAVVINSNNKPNPLATSSGPHQPDASTSTAPSTSAVSPSTSSGGHFYPSGVAFKTTLPMKDWGGANIHRYGILTAIGSSRRKTPPGCMPVVSELHGLEEWCRAPYNFAAGGTIGVSESTFMGIKNTVSKFLGFAHQFLGVPLPFLSLRLFSNQHITINFIDFMVARAGSQHTAVEITRLVKLVEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRI6</accession>
    <name>A0ABQ5RRI6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001667</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="2">
        <location>
            <begin position="467"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="723"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="723"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="815" mass="85299" checksum="E2D23F0F6AD1BECA" modified="2025-10-08" version="1">MAGGGHCTGSDHCSCWKPVVYVQVTTSLWRGTVITQQSSHRSMRKLSRSFRLQSSTGALKGPATALVPPPLALAAPSVLTAVVATIAVEMAAVAVVSQTSPLRDPQAPSPPLHPTSHQQQPAIPSLSQLLPVVPVKISQLPGLLPSPRASPLPPAPPPPVSSSPLPPRSPSPPPLPRSPPSPLLVRLPPSPRPPPVPRPPPPPRPPPPPSPPPRPSPLPPRPNPPPRPSPAPPRPSPHTPRAPPLPRASPSPRPNPPSPRPSPPLHLPPPPPTPGPTPGPAPSPTLTSLPPQPPFPPPQPTSPTPQPTSPPPQPTYPPPQPTYPPPSPSPLRGPPTIAPPAPRRSPPLPSARPPVMRSPSLPRPPRPPLPPPPPPRPPSPPPAPPPDNQFFSFSDAFDSALGSGPYQLPPAPPVSFAREIMQTLVLGVDLCGRVSGANMTLMQEVWATDIRTFFEKGSFGALKVSPDLNYTRVVSDHVAIPCSGTNSEGIRWGVTNQPNFATLKGWAEAADAAAKARGIPVQMYIQRIYLMPDSIGTNSWGTIDCPLGAALVRVWTGMVSGTDVALRLLVHEMGHNLGLKHSQGYDESGNLLEYGDGSCPMGAVPVPTHYNAAQSVWLGWTTPQAVLSAEDLPIGMWSVFAIRGLADSPFSSLQILPGTWTSKGWALGDNLYVSFRRYSHTNADAGLVELYRNKIQIHMHPRARDGCESPVLLVSLDAPPTTNAAAAASGSDGSGPASSGDSGGGGNTSSVWPQPGSALPQDKASPLLVVRVLGIDVDVGVARVALCRAAQFGPESGAQCTDGLDNDCDGQVDNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRK0</accession>
    <name>A0ABQ5RRK0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNL-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_002256</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024158">
        <property type="entry name" value="Mt_import_TIM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007853">
        <property type="entry name" value="Znf_DNL-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922:SF13">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05180">
        <property type="entry name" value="zf-DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51501">
        <property type="entry name" value="ZF_DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNL-type" evidence="6">
        <location>
            <begin position="253"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="207"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="412" mass="43159" checksum="38A250C13FDAF08A" modified="2025-10-08" version="1">MASKYPFRYGGLMLQDTVTSLDSFAKVYHSLTAWAPQWQRFLARSYPVNARNGGFILDGGAVLKFVQEHFADSCASFSLPTDNLVQPQSHNSPGKCKSVYSGQLRSTIAANATYVNNSNYCSPPRASLPSSVTFAITGAFTTCEARRQLLSHDGCTSITGATSFHHRHGGASAGYCTVAGVAIGSGRGAAKMSTAIGEGCGGEKNGLSQSHAAKGGAVEGRSADAHATGPTGSGNDAGGAAAGGGGEGSTLGRIQPRLMMVFTCTKCETRSTKSFSKQSYTRGVVLVRCPGCHRLHLIADHLRWFGEEPFMLHEFLTGKGENVIRVTMDDHAEATAPHPAVLEAQPRASAAAASLAMPGLTARTAAAAAAVAASEGGVFELGSELQVREALEAAREVQDHRHESKRQPLETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRR4</accession>
    <name>A0ABQ5RRR4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002361</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045401063" description="Peptidase M11 gametolysin domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="3">
        <location>
            <begin position="125"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="817" mass="87405" checksum="347FD72E4DEB8EDE" modified="2025-10-08" version="1">MFRKLLLAIFALFAASGVPCCKAAQSPPATTSNKVQIIGELINAVSDRGANQWFLRDVNGTTITLTGAYKVPDMGVDGAPIQGSVVQLACLFNATTGECLLDGSTSFNVMNPVKSPPVLTMVTLKLLVMIVDYPECGFPATITEDEVRSLYLGPNQDGEGGVAQKYEQCSNGQFVIDSEAFRAIRVPHKCSTPITSSCSYWAVEVLANAATKNIIGAGNFSAFTHYTYVLPPGLRPLCSWDGVALLPGRQTWLQTTPNGVYRWATVMQTALRNYGLWNAWRSGVEFGDNSTCMGRGEVCPNAAEISRLGWATAAVGGDQLDKDILLPGSAKTFILPATYLTGNYSYLRVVPDWLSTYDAIRGKNLYMAVRAAQNGDTALLSEFASKVNIHEVNATMDNSYPSSYLYNDRRVQFINATGNLSVLILNAYNLTVYGGSWVDVDTMRVHLCRFVNSPNECPPLSILEATNPANPVPSPSPVISLPPSTPLSPSPPPPLPPPPPRPRPPSPRPPRPSPPRPPSPRPSPPRRSPPPPPPRRSPPPNPPSPSPPRPRSPPPSLPPPPKIPPPRPSPSSPSTACPPVVGYSVTADTTHRGDDIGCDNISPASTAASQCNMDTSCRAFTTFTGRAGACIKNVSTPTATAPGVCLYTKTTTYCRLIPDYILTPGADMYKAEISCENITPTVAAQRCTESADCKAFNTYFDAGVGIPWACTQSGTGPLKDALGVCYYVKSNTTCPQVPGFTLAADQDHAGDDLTCDQTSLTDTISRCKSDYMCQGFSTLYSNLTTGALRGCTKSVTSPIISKAGSCLYTKVLWISIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRS2</accession>
    <name>A0ABQ5RRS2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF4215 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002181</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="332"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="39159" checksum="F5C479FB89777842" modified="2025-10-08" version="1" fragment="single">MTASSWTGGRLVPSVRPVLWLALFLQVIAFQRLAWGDSVAVIKSGGFVCLSIDVAEQLCTADDNDYCGRLYINMIKKALVMAPKDANGILAFGQFEPNSGARKALELWLNLAGYDTSIITYANDTSKFRSYNLNTYKLVYIPSGDYATSGGITGAMSDALQEMKSAFTSYVNVRGGSLIALAEPIRNEYRFAYGFLPVSMEVTVNYDKDDWITSGTVTKEMSIYSPTTTSSSLNERSWLGYFTGPTDWNGLRVVAHVPDSCPVPQGKYQNCHATVLCNTQALLTSENCYNGVDDDSDGAIDKDDEDCKRCGDGTIDSNERCDDGNILDGDGCTSTCELPPPPSPPSPPKPPSPPPAPPSPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS78</accession>
    <name>A0ABQ5RS78_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002237</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF20">
        <property type="entry name" value="SHSP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="40"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="144"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="462" mass="49966" checksum="A10C6C0A7F586C4D" modified="2025-10-08" version="1">MDTSGITKLAFKQITDCHLPLYRRQDYMTAAVYPSLHRQVSLPSRDAQPPSGRTVALLPPYPSSRNHSSLRAMPISAATQASLTSSTTTAPAPAGQRSGSGRRGVGGMAGSNTENGQEQIEIIGRRTFLACVAFITASFSADIDTVGEGDGGERRGLAHAAPSSSTPTSPPSTTPTTITLYSAETTTVASATTSVARAVDLTTIQEEAFLAYAERRFQDAVNLLTECITAEPDSARWREMRAEVLVDGKNFPAAVQDFNAALALLPDKDLVTRARLLSGRALANEGLSDWEAALADYRRGMDLAAEAGESLDPYVLNSIGNCLNSLGRWADAREQYLASAAVFQQARGFRARNGSSTPRLDGSIFASSNAALMRAQMGDTDGAVQEMVRIARRAPGSADMRAALAALYWGQGRWQEAEEMWEFACDRITVGCTQYTDLDWLGRIRRWPPLMVHRMEEFLALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSY7</accession>
    <name>A0ABQ5RSY7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002811</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0157">Chromophore</keyword>
    <keyword evidence="1" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="162"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="218" mass="24409" checksum="813F01CE9D3ED9AB" modified="2025-10-08" version="1" fragment="single">EARKLEPNFMCRFIMFVRHQQASQLAAGKQSNGGTNMDLLAYVEYQRKQRMVVRLHKEALQAICSFWKALDSHLVSFTTLSKGLSKIESSVSKAQAAYRVVLETYGNNPKLVRLYGKFLQSIKNDPWRASEYFAEAERLEEVQDGDTSQGPLLPDGTPLGRMDDMATAVLVINAAGEVQVANRHAHVLFGYKRGTLEGKPVASLLAPHFARWFANHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSZ5</accession>
    <name>A0ABQ5RSZ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002482</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027193">
        <property type="entry name" value="Noc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455:SF0">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="418"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="523"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="325"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="683" mass="73100" checksum="3170CA5DD6EC1274" modified="2025-10-08" version="1">MSELGVVDSLVSELLAGGRTKANNAAKLLVFMSTCKQEAALKAAIQGLKHFFVAAGQDGLLTPYSTGIRLAKRQKIAPASDGAQETAGDGTVTQDDAEAQYLEWLSRQYVSFINQLLFLVTGVRQTAAAGGTGTAPPPLPQRSAGVQVVASAALMECVRSELGPGMFSGSLYDRLLSGVLLSPGVRPEVFALLFTKYLQLADVRFFTLATVKAVASRFSSSAGAQTAASASEAGKEDEEDEVAGATRAAAAALADAANANSSSSGTAVSLPDLVRNMYDVLCHVTETLPTDTQEWSTWCGAAEVNLVTAAADKSESARLRRKRKEREEREKQQQQKQHQDQGSQQQPLQRKVQWANPITQRRLYGDAWLALLALPLPVDVLRKALVRLPSAVIPYMMSPQLLADFLTHCLNRGGLTGMLALNGLFLLVTRHGLEYPEFFAGLYQLLVPEAFVARARAQFFRLADVFLSSSLVPAYTVAAFVKRFARLALGASPAGAMIAIAFIHNLLRRHPALNVMLHKPNPSSVPSGNGAAASATLATRPSRELAKPVPSLSGPGVDPYDEMEADPAKSRAVESSLWEVEALRNHYCPQVSTFCAVLDKDLTDRTKTAEVNLEDLLGGGKGNGQQQGGAGSYSALIQGELARKLRKVPVAFYPQPPTALFPTTLAADDMAGTHFKQERPAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT61</accession>
    <name>A0ABQ5RT61_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ, chloroplastic</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="7">Glutamate N-acetyltransferase</fullName>
                <shortName evidence="7">GAT</shortName>
                <ecNumber evidence="7">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="7">Ornithine acetyltransferase</fullName>
                <shortName evidence="7">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="7">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="7">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="7">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="7">N-acetylglutamate synthase</fullName>
                <shortName evidence="7">AGS</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_003015</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(2)-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Plastid</location>
            <location evidence="7">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ArgJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="7"/>
    <dbReference type="EC" id="2.3.1.1" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2330.10">
        <property type="entry name" value="arginine biosynthesis bifunctional protein suprefamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="entry name" value="ArgJ beta chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.70.12">
        <property type="entry name" value="L-amino peptidase D-ALA esterase/amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003802">
        <property type="entry name" value="PRK05388.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100:SF0">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="DmpA/ArgJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5044916557" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="7">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044916558" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="7">
        <location>
            <begin position="283"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="7">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="7">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="7">
        <location>
            <begin position="282"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="502" mass="52096" checksum="626F7768BEA9F52C" modified="2025-10-08" version="1">MFQRSSECKAPYGMPSLVYLSSRTQIYMPPSGPFKCSLKMLAQQILQRGHARAINHAAIKIMPRQFCRTQAILTDQECYTINAAPDLIPEGAWSKVEGGVCAAKGFKATGAHAGLRASGKKADLALVVADAAATAAGTFTQNVMCAAPVLYCKDVLSRTNTIRAVMTNAGQANAATGTQGYEDAVTTAKVLAEALGCSTDDILLQSTGVIGRRMKIENFLPAIPELVETLGSSSDDAHRAAVAITTTDLVSKEAALRVTIGGTTVHVGGMCKGSGMIHPNMATMLGVVTCDAAVAPDVWTGIIKRASIASFNSITVDGDTSTNDCVIGLASGAAGNALVTDPASSDAQLLESAVTALMQGLAKSIAWDGEGATCLVEIEVTGAKSDADARVIARSVAGSSLSKSAIFGHDPNWGRIAAAAGYSGIIFDQSELGVQLGSTKLMEAGQPLPFDKATANKYLKDTCAVHGTIHIFVTVGKGPGKGMAWGCDLSYDYVKINAEYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT72</accession>
    <name>A0ABQ5RT72_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="213" mass="23317" checksum="CE723168AD086E10" modified="2025-10-08" version="1">MSTPTGVSGCAWRQCSPSIARPRAVRRTMPCRAVYQTEAGMPPQPPPPPPPPPPASFTRRNVLQQFGGFGAAAVAGGFMGYLLGSRSGGSDNGGLSAAERADVDELRRELHFRRDYDGHVFVRDGRGAWWEVRLDAKLPGTMLLRDPQDGVFFITYNNIQQIDLTDDYVVGSLFAGGDWESIAQRLQARDDMGALVDVKLGKEAFRDMISVMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTJ3</accession>
    <name>A0ABQ5RTJ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_003040</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039745">
        <property type="entry name" value="Vps54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012501">
        <property type="entry name" value="Vps54_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019515">
        <property type="entry name" value="VPS54_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07928">
        <property type="entry name" value="Vps54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10475">
        <property type="entry name" value="Vps54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 N-terminal" evidence="10">
        <location>
            <begin position="238"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 C-terminal" evidence="9">
        <location>
            <begin position="943"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="173"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="791"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1237"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1640"/>
            <end position="1678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1729"/>
            <end position="1981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1997"/>
            <end position="2031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="795"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1243"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1253"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1282"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1319"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1429"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1450"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1652"/>
            <end position="1678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1782"/>
            <end position="1797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1798"/>
            <end position="1838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1862"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1883"/>
            <end position="1895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1896"/>
            <end position="1906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1916"/>
            <end position="1948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1956"/>
            <end position="1981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1997"/>
            <end position="2008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI60895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2031" mass="207920" checksum="241D76CDDFA887CA" modified="2025-10-08" version="1">MKPEQQPNGPAPNRQPYQGPGPPPQSTRTANSSTGLSSHGTSRKRLGSSHFDRSAYTAGQTLVTVVNDPRADATKNWSAWLGFRVPDVSWQDPPPPIDTMRYPPVTRRDLARYLALISDGSYDQFQQLRQSLALGGISQQMMGLGGDSIGLEDDDSESYDLLPYEAYNGDAAATTEVDGDPDQQQLGSYVGGTGSRQRSDAGTQNESSSLPGPSNHRPAAAPPRLKQGDGLVTALQVVPRLFFNEDFSLSRPDIFEAICGCEDDSEMYERIDKLNGALDVVETQLMREIASRSRTIVAAGNCVHDLHNRLSSTLAHIKRLRENVSDMDDVTYSAAVRVAQLQRRRNNLSATVERVKSLEEVAAAKAALPLLLESGDYAGALELLDNLSHAAAGLSSSGVAAFRSLAPQLADSRSVVESLLTSEMLGAAMYTDAHLVLERAVAESAQALGLAAATATLALSPADNNGVVSRLLSSASSPSPSFSAPPASGLLSRECSLRSSGSSSLLAAELAAAREQLHDRLLPVVLGLHRCDRLEAALRQFAVNRAAEVKAIIREVVERLLPVHLQLAGFTMDGGGGGNGGGSSGPAATGGGGAGASGTTGAAAASIADGGVSGFGTEMPLAEKLAVLPHLGFLQILLVINSMVEAYLAHSLLVGTLVEDALRSGRAPAAALAAPRREVHSALQAVVDVSTGRWAKLIAARSDVHSRLKVYEFRALLSACETFAGLPDLHGVRPGAALRPTLQALCRAHLEKTHARSAMQMQHLLEAEQWVPAEAHASFQAIVDRLEARCPGARQPPEQQPEQQPEQSGQPTAAAESSTSSPSSSSSALPPEREATALKEGARAAGVNAGVVLRPTEGLLRLGGRHYWPVNALLMLLRLLDEHYMGLLPSTSPPAVAAASAAGAASESIKPAAAAAGALLRRPLGPIGQSSSAPAQAASWALSTVGLDVAQRCLELLRSFNMVTAQQVLGAGAMRTAGLKSISAKHLALAAQSLAALLAVLPLLRWQLAAAISDPARRALMMPEFDRLAQDLNLHVEEIHGKLVDIMQDRVLAACRQVSVDSDAWSRADPTLQARQVEQPAPSEALRLLARQLNTLRSVLQPILQPEEVSYIFGRVAAACSESLAGLLDSLPPPSVAAAPASSSALTPPVSSGASGLLRGFGGGVAAAAAAVAAAAEAQALAMAAWEASRRANALFMLQALSTLPLDPSRASSYVTRLATFYTKHYGLLQAPTAASTATETAAPPPLQPPFPTGASPLPSAAAPSGRTGAASWDTPASVMTPSPPPPPAPAPAPSAIIGSEARSPMPPEPGRGGPANGPPASFAASGGVGESAAVVTGISSSAPSNDPAPSSSSSSSSLPVAPPQLQPQSPAAATMSVVMAGRPGEEVGLSGGIPIPPTSPMPLQQDQDQGPKHGGGQGEGEGERGGEGEGEGEGEGEGQGEQQQSQLKQPEELRQDDDGRTVITASDFMTHVDAPTPVSLPAPAPAPTPLQNSIARNVPPMYEGGERTRRMEEEVGVGVLTEYGTVSAAASVLTSEAAPEPTPPDLAALSPTAGGIPAITEMPESPAAAAVAAVAGNDDGEEVGEAATATAVVAATAIAAATPTANPTASAIANPAAAAAANSSEVLIYMEGSSRFAEGFGEGPLDSEQGEGAGGSGSGIDDGSGSPNGGSGYSGGKISGERTISGFPSGAGSGSVAVAEVVSSAALAPVAVTEAVFVREPQQLERLSASEGYAGDGKTSPGEEEGALLDMSSGGEMAGERSLEGLVLRDSAGEVNSEQKQQQEEEKREEEKREEEKREEEEKREEEKREEEEKREEEKREEEEKREEEEKREEEEVALGLLNDESSPEDAGNGEAVWAASTSSLNTPSSTPQPALTEPALAPAPAPAPEPALAPAPASASASAPAPAPAPEPALAPASASASASAPAPEPALAPASASPSASASAPAPEPALAPAPASASASASAPAPEPALAPASAPVPEQALAPAPVLVAAPEQTPVSPPAASTAPPPPVAVFFDPLGGLQVSRGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTU1</accession>
    <name>A0ABQ5RTU1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase M60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.80">
        <property type="entry name" value="Peptidase M60, enhancin-like domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.30">
        <property type="entry name" value="Peptidase M60, enhancin-like domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035423">
        <property type="entry name" value="M60-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042279">
        <property type="entry name" value="Pep_M60_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031161">
        <property type="entry name" value="Peptidase_M60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051244">
        <property type="entry name" value="TCAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15730">
        <property type="entry name" value="EXPERIMENTAL AUTOIMMUNE PROSTATITIS ANTIGEN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15730:SF5">
        <property type="entry name" value="SI:CH211-210B2.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17291">
        <property type="entry name" value="M60-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13402">
        <property type="entry name" value="Peptidase_M60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01276">
        <property type="entry name" value="M60-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51723">
        <property type="entry name" value="PEPTIDASE_M60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M60" evidence="1">
        <location>
            <begin position="421"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="793" mass="86388" checksum="D01D89534F73CF74" modified="2025-10-08" version="1">MIYNARRMIIRARSTWLESTFIIQAFLLTMAAAQQIPIQTSVLSNLSAALPILQNGISGPYPRVAYSSPVIIWSNTGFPVALGDDGSNPLPVVAAVVLPSTSKVVTFGHESIPGSCCSTNDNWDKLTVNAVRWMAGSKKAITIASPGSSSWWSSSGIAGVQKALTGISVTVKAVDLDTFASTAWKTVDIYWIDTYTSYTSSQVAGLLKFANQTGKGLLVGGHAWYWSYSHPNANIFTDLTINKVLWPLGLAVTTGSQAGFQAAPNTLPSSWIYYNTYLVASILSAVKAGRTTFTNAALYPTANSALNYLVNFLPPSTSSSASGLNQLWSLLSSARPSSGTVVSSSNPLDPKNSYPGKVLDVALEVWAIKRGDLASMKSSASASAYPGAIPANAAKLSVDITINGTYNPPPYPFGYASWDSPVWRSTGLYAPPGQVISVIFGTPNAVGKMLKVQIGAHTDDLSGKPTWARVPSIVSSFDLNAFITSACNPFGGLVFLLVPPGSNLGNVKVTITNVVRAPYFQYNKTLLSDWSTTIRNYPAPWAELDSGKIILMIPSSVIRNLANPVPLMEHWNIVLDNMAYLANMPTERARAERFLVDVDISNGWMHSGYPIMAYDLPGVHQELTNVTILKTTGSWGPYHELGHNHQWLDMQFSGTTESFNNLWTVYALEKTGTLQNTENWNMVSPSGRASLRADYFAAGANWNRDWNVWVALDTYLQLKDGFTWEFYRKMYATYQQMQLPLADDNVQTWIQTSSKVAGFNLVPFYQKWGFPVTSKTANIVGLLPAWKNNPLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU44</accession>
    <name>A0ABQ5RU44_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002994</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="534"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="619" mass="65801" checksum="C13F881C47514174" modified="2025-10-08" version="1">MSRRPCGMFLCPDDSMDFLPGTDSLMLRTGSDGNTGVHIISCQEMLLAVSRLSDDGVQQPDKALGFPHRLGLSSSNTSTSSIGLIVPVDRIIFRNPGQLCLSSVGPKAGSIGNVHHADCGSVQLADLLTFGDTSSSFNLVSMSSCDSQSELADLSQAAYPGLTGRPSLVPFHPPFTPLGCDNDYLQSDMLLEEGRHHGGDDAATALRNESARLRSAVQDSKSVNCTTTSHALPNPLGNESLLVRDKSTDGGLNHSSGSQEHSVEAYAARLFEAADIVDADTATELLTATITPGRWQCSSWVGSTGVLPDDGEPFEGTASLQMNQRSFSGGLLSASMSEAEAGFCRPICWMKSEAEACATRTPSGSPSGGHWAIFAASGVAPDQVFTPSAPQSMPQSSRATVLQSSGAAVTETGMEMSPFATHAYEGFLMLDHELPDDSVHGGKPRHPSLPAAQPCDNLLLEWAEAITNGMHRTGRAESGTFMAAPCSTGTPATPSAVRNNVVGADRLLVAQHCCWRPRLRDPQLQLKTLRTASIWMLLPVLAQRVLLACLLLPVLVMRFAVLQPLAACGWRLLTLMTLCSAVHGLRSVEPSSPLPDAQLRRPQPWTAGQQDSGHYQCLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUB9</accession>
    <name>A0ABQ5RUB9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="531"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="578" mass="58718" checksum="DBEFE08B240FF2A4" modified="2025-10-08" version="1" fragment="single">MLDDDDDDDDFFKDFKVDLDVDMLPPAPKSSASAAAAPAKAVPIAAATHIGSDAEDDMDVMDDADVDLLMSDTDAGAKVNAAEEKIEVTGERGSVQILPLLLPPTESAPGASVAPPTQNPQPQPQEVLVPDCGGAAQPCPPHAPTAETGEGYNAEAAAIAELPVPARAEPEPGIVLEPGGSGRCYDATYGEREACAAIPKDCVGAAAADPAPQYLVSNVAAEAMTADATVTGARESGTDYEEIPTPVVEIGTTAVDMEQPEGDPQVGQAAVEDAVGPSNASGNGDGNNGNGRNAQEMAQDAVGATILLEARPSSPSLAPHSNVSEIAAAPHLPRDLITASSHGAPCDGMQQLSQPAVAVATGLCMSGTRTKRSAATPNETLPLPLQQGSSKLPIQQRPALKAKLPMPLTQPQRQSQSPSQDSIAEVALATPVASAANLPPQAQTGSVIIAHAASLQPLSAAIAAPSVAVPPSAGAGGAIIANGNATGTPPAPNPARPLNRLDQGRLMGRSLVGGLRGPAASSPTLQAPLSLHQQPQKKPPVQLQQQVPPHHHQQRLSSQQQLQGSAHVPGTGMRPEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWF7</accession>
    <name>A0ABQ5RWF7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003976</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF100">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="29"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="422" mass="45953" checksum="96147656C95FD21C" modified="2025-10-08" version="1" fragment="single">MQNMMSASDVRPEGRTYIAGRNSLKVISYNILAPKYASYNSYCPPKFLDWAHRRAGVLRELTHLGADVIGLQECDMEFFNEELKEWMALWGIRGQFLERPVGPVPGLSEGVALLWQDALFEVVEVRQELYSRMDPRVAGLPSEVAGTRAWSKLREMGEGLLMALLRHRPSGRLILAAVTHLFWDPAFPDVKVLQAALMCGSLSAFAREAVGAGGVLRGPPPGLLLFGDFNSLACKYLPDKFDTAVPPGGLRSGVYTLLGQGSLPPEHPDHPATRVWESEGPCPTGEQHFPQLPLTSAQLQFTSLYAEAFGREPPLTTRTASWAGCIDFVWLSRGDFSVASALMMPYEDGGLPPLGPDADATGRRGRGSRAPSWRDPLSDVTFGPIPDEFFPSDHLAVGGDVVVLPPPPLLSSSNIMATAAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWR6</accession>
    <name>A0ABQ5RWR6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004186</name>
        <name evidence="8" type="ORF">VaNZ11_004197</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="11652" checksum="4AD26F08965BC8F9" modified="2025-10-08" version="1" fragment="single">GPYSENATPVYLTGEFPGDYGWDTAGLSADPETFKRYRELELIHARWAMLGALGCVTPELLSKNGVQFGEAVWFKAGAQIFQEGGLDYLGNPSLVHAQNIVATLAFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWZ7</accession>
    <name>A0ABQ5RWZ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxisomal membrane MPV17/PMP22-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004014</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF80">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="29165" checksum="94DABC7C6AEB091D" modified="2025-10-08" version="1">MASHCISQSLLRTYLQTRHFHTSAIAGLGRRRCVSSGKPVTPAPGSPIQRRMVCRAVEGSGSGSGGGSAGRKPVGFSGDGGHRKPQGASVGLWAWYMSCLESAPLLTKALTCALLNALGDVFCQLFIEAGKWDIRRTAIFTFMGLAVVAPTLHYWYALLNKLVRAGGTTGATIQLLLDQGVFAPVFLATFISVLFTIEGKPHLIKPKLEQDLLETVKVNWVLWIPAQYLNFRFVPPNLQVLTANVVALIWNTYMSFQSHKAVVPQAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX30</accession>
    <name>A0ABQ5RX30_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="102" mass="10808" checksum="C142FE8EBFC06B0D" modified="2025-10-08" version="1">MSLPLASADGLTVFGISSIGAQGHTARGHVGNGSPAANIEHAVRYGGSTWQRRVSMVDDRLETVGGAEAAVLTHLARMVMLCRSGVRKMMFGRLDHPALPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX73</accession>
    <name>A0ABQ5RX73_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022111">
        <property type="entry name" value="Rhodanese_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020936">
        <property type="entry name" value="TrhO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040503">
        <property type="entry name" value="TRHO_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43268:SF3">
        <property type="entry name" value="RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43268">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE/RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12368">
        <property type="entry name" value="Rhodanese_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17773">
        <property type="entry name" value="UPF0176_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="219"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="489" mass="54222" checksum="E0460E6A51BAE770" modified="2025-10-08" version="1">MLLSNSIPKSCKFLTHERSVPLSLLMHPLALQHGCASPVPEWRQQYQSRLICRSNDSGFHANALASSISPAAAKSSSLPPQLVPPTDPSAYCVLNFYHLTQIADPQATVEEHRRFMELLGLNIRGRIYVSSQGINSQCGGTVEHATAYAEWIKQQPGFQDLRYTIWPAEGHAFPKLRLKYKPNLISLAGGMETMPVTDPAARATPLNPSRWKEMIAQAQEEKMVVLDVRNDYEWDAGHFEGAERPAEEVFSETPVGEGELEVPVYLKEVDKDTPVMMYCTGGIRCDVYSTFLRKKGYSNLYTLEGGIQNYLRQEGGAHWKGSLFVFDARMAIPASGTETDALPAAVPCSVCGSPDSQLPHVNCANIDCNELFIACSTCKTRYNGCCCEACTMAPRLLRPAKVDGGHYGAWGNYADLDEMGPIISQGRTREGRVARRARRREALKEKRMEFVAKKLARKKMMWDAMARLEALEVQAPQQLDGGVKVACAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXC2</accession>
    <name>A0ABQ5RXC2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004888</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="256"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="584"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="525"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="625" mass="64940" checksum="BE8472F733582F27" modified="2025-10-08" version="1">MATVQEGTGRTARAKGDGQSASAGNNNSRSQGKVAFKEQREEYEYRGHYTDSASSDSDETSCSSSDYSSGSPDRGRHRRSYRRRSDRDRHRRRHLSGRRCRRELQGTSSSSSYDVHAAAATAAAGAAAATAAAAASGAPGAVGVQIANGPGSVHPYQLPDQPFSMALMPPLGSGPFPASPPLAPSNPTKAYTSPRSAHDPPPMLLLSAYGGHHPNCPAAVVYGTTPTFSGGVAARPPASASLLAPMQMLAMNGAVLPPPPPSPPAPGPGSPTMQYSGGVMTAAAAPPPPPPPQVLTLPPPPGALPLALPEPMLQLPYAVQQQQQQAAGGLNMLYDETMQEVVLADGVMVKELAKQGHGLVPLADAHRTKLAALQAAVNGPLLAKREALTQQHARLAARAGEVAARRAALEGEVAVLAEDMVGRIRGAESLKQSVLAREQADVGAQLDAIQRLLGDIMAASAAGPVDFLNAYSRLTGTCNRLVSKSFKQVVDVAADDLPAEAALHRDLAASHEALTQLLGVKDQMISHLLQERDALQDELRQVVDRYTGELSALEAQCQGYYQRLTALGATTANNAAAAADAAGRSLGRSPPATAREMEVLEAGGGGGGGTDLFLRGSPSRTHPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXD5</accession>
    <name>A0ABQ5RXD5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein FAM221A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004898</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM221 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026755">
        <property type="entry name" value="Fam221a/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31214:SF2">
        <property type="entry name" value="PROTEIN FAM221A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31214">
        <property type="entry name" value="PROTEIN FAM221A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14753">
        <property type="entry name" value="FAM221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00039630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="249" mass="26023" checksum="0C91EA8E5184A32F" modified="2025-10-08" version="1">MSAAKMNALTNLKTRRQQAAALGATETDAPPGSGTEVGPNSAGATGSDTQASGSLRIDPNSLPTGAAGAAPAAASQPSPAPSAQAAAAALPTRAVEAFAWVCGTCQRECVPVRSESRCLCGHRLREHDPASSKIPSRCKAAKCSCRGFFFIVAEGAWVLRCRCKHKHVEHDAATRACAKGGCSCRGFDSPWVCNCDHPWSDHRQLVQVKEVVSVRDMMGTMTLAEIGGGGPAREVNDYTALKRGDFGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXT0</accession>
    <name>A0ABQ5RXT0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_004999</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07841">
        <property type="entry name" value="STKc_CDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037770">
        <property type="entry name" value="CDK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF0">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="5"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="286"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="341"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI62380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="362" mass="40079" checksum="68979BED7C025BA8" modified="2025-10-08" version="1">MAENYARGDMLGQGTFAMVFKGQDKRTGKMVALKEIFTDEKGTDGKKGLDPTALREIKLLRELRHPNIIRLEDAYPKKKSVVLVLEYMHSDLEAVIRDQNLVLAAADVKSYMRQLLLALDTCHRSWVLHRDIKPNNCLIAPDGSLKLADFGLSRVYGSPDGRLTHQVFAQWYRAPELFFGARQYTSAVDVWAAGCIFGELLLRRPLFDGMCDIDVLSKVFALLGTPGVEGNWPAAKDLPYYLQFTETKPVPLRQVFPAASGDGLDLLSRMLQLDPLRRITSADALRHPYFTNNPPPTPPALLPRPVKREDSQLLAPGQTKGARDRLSPGATSPRPTKVPRRSGATGAGSSEAAARPSPRTQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY23</accession>
    <name>A0ABQ5RY23_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004748</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="119" mass="12991" checksum="E771C56D902090AA" modified="2025-10-08" version="1" fragment="single">LYLAEADPTLAAAGRAARVGLLVDYYVQRNYYAAYTFTRFGPSAVSTTAVTARSRGQVGVAAKARALAQALFNYRRSRYDGNRVEVFLNTRGLSTTSVGAYLFNNPIVNNQFLLQGPNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYB1</accession>
    <name>A0ABQ5RYB1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004608</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="3">
        <location>
            <begin position="562"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="372"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="665" mass="71750" checksum="44A769D8C267BAE8" modified="2025-10-08" version="1">MYDMIPKGLLMKRSYGTVNGKWPRGPVAQRHRRLSVAQSGRGPTPSSSDDPLKNLDLKSLNDLPLSKLSETANAVWERIRSSGLNVKVNPSSSNSQLLSLKLVPGAKTSAVPLSNGSSRTPDMSDLALSEKSGSGKEQRLMAALAQAQEEKRQLADRAEVLHANMSMLETKNAKMAREVECAEAGRANALKLRDMLLDERDSLAAQLKEAQNELRRASEGMEVREGALEALRQSQLQLQDELKDAVARLQAVDKSTEELRSQLAALEGSRAAVANERDDLASHLRDLMGRVEHVSGLFSGEFMEASGATAAVAAGEAPTGGGGGEGELSRGGAAAQLEQLRATTEGAFAALRMARSELGVLRSENQHLAQRAETLTSSNRELVAMAAELKQQVTEARSELDEQQRETAELRNREAALKMELTEAVSERQAAVAAVAAAEAQRNDLATAVAQLTAIRDAVAASGLSLVELAKMAVQLTTLKDQLANGRASEVDLKRRLATAEVALLDNRRAAVATEARMVSELEAARRDAAELRSALHARTLATQATRRAGVPLDRTSGQLINTNRFWVPADVLFDFVKAVAAREVALVEAKGFLDISVHSEEHNVMVVSTHWNSPAAFEAWSRSAAASRHHYPAGVYQYAPRRGEGVPEDYLPFKDVGDPARAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYE1</accession>
    <name>A0ABQ5RYE1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004489</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23294">
        <property type="entry name" value="beta-trefoil_MIR_AtSDF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809:SF2">
        <property type="entry name" value="GH21273P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809">
        <property type="entry name" value="STROMAL CELL-DERIVED FACTOR 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046809331" description="MIR domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="4">
        <location>
            <begin position="25"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="4">
        <location>
            <begin position="87"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="4">
        <location>
            <begin position="145"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="218" mass="24227" checksum="E625FD5102F651A4" modified="2025-10-08" version="1">MEGTTRRYFITLVLICVNICVRADNLLVTCGSTIKLQHVATKARLHSHQVAYSRGSQQQSVTGFPDGDDGNSLWLIQGIVDEPCTPGEPVKKGTRMRLLHVATRKWLHSHLYNSPLSNSQEVSAFGSDTQSDGGDVWTIEWDGKGKVWKQNTKVRFKHVDTGAYLFSHDAKYGNPISGQFEVAGIPNKSKNTEWVAAEGVYMPRYSEKGAADDDKEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYK0</accession>
    <name>A0ABQ5RYK0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="186" mass="19945" checksum="3168C1934ECBAE71" modified="2025-10-08" version="1">MTMALVPAHNAWTTGLCDCAAEPSGCCFCLYATFCQPCAYGTNVELMEPQLMWCGGNCAAACLTYYLVGAFTGCACILQVVARGYIRRKYNIPGDSCEDFMITWCCTPCALCQEHRELVIRGHKPGGVVPSVNPQMAPQPQMMVMVPVPAPMPGGPQPGYPVVMQAGYVQSPPQPVPMAYPAVPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYN5</accession>
    <name>A0ABQ5RYN5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004747</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053303">
        <property type="entry name" value="Chloroplast_PPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47935">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN MRL1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47935:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN MRL1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="256"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="341"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="376"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="448"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="895"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1131"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1270"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1318"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1131"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1153"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1172"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1282"/>
            <end position="1296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1393" mass="148103" checksum="43A3D74D502FCB65" modified="2025-10-08" version="1">MAHSAAGNAARCQTVAFREPHPPRSCLLRVSAPRLEQAEPSPQDEVPITRARYSAGAFAPPATPILAKDTSRPTVAPLRGPHHIQQLLAAEDSWQRRTPLTDKQLEEFSSLVLGSSSNVWNAEERSSPASTSDREHSVDGFVAVLKGGRGVHAALTALKQLTRRGELEQIVRGLQELGKAEQEVIVRRWRYQYFLEAITASQKAKLALQFAKLAMPYVHKPALLTMSVLKACATSRDLDAGMTVVELARKHAVPVDSHMLTSLIKVCKAVGNVDKAYRVYLDMRAAGLRVDSHVYGALIATCAEAMKRDLTVMHERKDQYVLLERAFQYVADAEAAGVALQAPVWNSLMVCAGRSGELNRAFEVLTMMQQRGISANATTYGSLIESCVCARQPEKALRVFEVALQKGFQSEVKIYTQALSACMLPLPHAWERAQDVYAALQRASVRPDKKFFACLMAVAGRCGRLDTSFELLTEMAAEGIRPSSTTISAIIYACLERGNLALARRVYDLGVRQGVYPVLSQFNRMMDVYASECRFGEVVSLLSDMVGAGCSPNLNTYRIIINACVFTDQAGLAFQVFALMHANKVQILQLSVAQAIYYMLVKACFSQTRLLWRPAGYPPLGPQVWTPAAAAGGPASQPMLTYHHGRGLPAERQKEAERVLAALGGHKLRRGAHQSNPFDGTPDTIDWASHALSAFHHMLSRGFRPTLELLDGLLSCMRAMMMPPDEPGSLESASHYVSPFVPLRSRDEVFELAFEPRAFAVLDEAIAKGLVPTYDPEAPMVVDMRNMPPVVAEVYVLHVLLTLERRARRRQQAAEATEAPGVGSYHHSITLLVPPFDPDLVKWPSYVDRLMNRYTAMISSDERPRLRHRSEYRRARSRRRRRRIAAAEAAAAAAAKAVDDDGADSDSDSGLGPLGGVASSSYDELEAVISGSDDDMPSMVHHDPWGRGEDIMFDNGEGDGNGGTGLGGASGELYVADSSTGLAVAATLQRMKVWMDMDYVNGTITVFAVEVTRWLKRRRQAAEAASAAAAAVTAGTVAGANGSSRRSGGAAAADVAASDGVDNVLSSPGGGLGSPSAGRGAVGSAAVMGMVGGGLAASLTSARMMGIGGVRGRGTPVEQQQRNIRLGMMSAGGNGGGGTTGRGLGAGPRPNHALSSQQQQAVAASFNVNSSTPTPGQPLAQAQQGGRSLFGSQGGVESVRVRPQGAMQPSPTRSAQRPLPLPLTDERIKETQHRGGMAGAASAPVASHAELSESHIRQHDATVVRSSQPVHHEGSAAVNGHLPQSNGVSTNGAASNGVTSNGATVNGGVLHVTAATSQHQLQQAGHGHRRSSSGSVPPTESLLVSATQQYVEVTVGPGRPVMLQVSESTMISAAAVAAAAVPTPSGAGAGSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ68</accession>
    <name>A0ABQ5RZ68_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_005370</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045348">
        <property type="entry name" value="CPSF4/Yth1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102:SF24">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="9">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="9">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="154"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI62694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="362" mass="37812" checksum="0C2A80EB913FFD1E" modified="2025-10-08" version="1">MADNISFDFEAEIESSHLLTNAQQPIEPPDLPAEVIGQQPANYRKNFRKTVCTYWLRGLCMKGDACGFLHQFVSDRMPVCRNLLKYGECHDQDCPYKHSLDEIKECNMYKLGFCIYGPACRYKHIKNPGPPPGPEEVEAAKPREYRNINVVVNQVNPGVAREDERPPKRPSRPFVGRERERDRVGERERDRPLSLPAPPPPPGGPGGAQGPVGAAPAGGGGVGAGVGGRFDGRPGTEGPSGPGGRGGRGDRGGRSGRGGRGGAWRGGSGDDLDLGGLQGRDQAPVGMPPVTAPTPGALPPIAGLIAPVMPAIMPGQAPGQGVPGGGPAPMGPGAGGGGGPMGHFDSDLFAAISGQGFFPRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZD6</accession>
    <name>A0ABQ5RZD6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26420" checksum="AFC52349CD97A80E" modified="2025-10-08" version="1" fragment="single">PTYVKMYAYVKMLQCEELDEFVDSTFQMPTVDDPTYPAVQKKVKRARAIIIMRLTAKDHKSTIDNSTQAKDLWIGFKGWAKKLSDAELTAISHNFAALKMVSGEIMHDYITRALDMFNKLSKHSDFVQTVRNGITQIIRGLDDELYATEKVVVTYAQPKDFDELKERLLGIEAERLAKRKGQSEPATALYAGHDGGHKFRGCFTCGGDHLARDCPKKGISSVAPGSGRSSSGSRLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZQ4</accession>
    <name>A0ABQ5RZQ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_005374</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0677 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011610">
        <property type="entry name" value="SAM_mthyl_Trfase_ML2640-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00027">
        <property type="entry name" value="mthyl_TIGR00027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43619">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE YKTD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43619:SF2">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="38089" checksum="D13F1DED84270C83" modified="2025-10-08" version="1">MAASVEATPEAVVAASNDNKGSSKLGLLYYITRGQPTNYEMLVFRALNRDLRLPNHNDDYLAARLRDELMPVRSWMLKHLPGSKSQLKRTLDNPLWGAPGAVNFIDARTKWFDSAVRDALAAGIKQVVILAAGYDTRAHRLGATGVRFFEVDLPSASSTKKALAEKLGFAKDPALRPVYVAADLSRVPLAEALADTGFNPRLPTLFTLEGLMYYLPADACAALFHSLASLSVPGSRIYFDFMAAAALEDRAKFPGFKVTRKSVANKGEPFLSAIEATREGVMGFLVPFGLRLLDFLTPKDMVGRHLPHLQWNERVPPIASFYFYAATEKPRAAANTTALACPANATTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZV3</accession>
    <name>A0ABQ5RZV3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006038</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF1">
        <property type="entry name" value="FI18259P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="299"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="13"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="317"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="340"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="39225" checksum="F0723CBA65510554" modified="2025-10-08" version="1">MLPYCRRPRRSSAGGGGGSGGHGGGSGGHGGGSSGHGGGSGGHSNGGGSNRLLGLSTGWSVIRHSSFYRRIAAIWVVVAMTFEGAGELLMQYLQFKLGFNTLDQAHVLTVLASGGLVVKLGLLALMVRWMGERRLLAFGLAAYAAECVALAAAPTKAAGLAAVSIGSLALVCWPSLTALETAGVDPTHQGAVFGALQAISSMASGLGPLLFAAVFRQATRSDSGGRPMPGVVWYLAAATTTVAIGLTLSLPQQPQHVHPHQHTRHCGQDGGAHVAEGKGGCSGGGCGRQRCGRDVKCSPDVEEGGAGSGSQSEEEGLEHHHLEQEWVRERGAAAGSGEGRGGEGRGRGDREPGQYPCKVSQPTRTHQVCGMSEGLSLYYVTSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0B9</accession>
    <name>A0ABQ5S0B9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor Tu, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_005427</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04098">
        <property type="entry name" value="eEF2_C_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04090">
        <property type="entry name" value="EF2_II_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01683">
        <property type="entry name" value="EF2_IV_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16264">
        <property type="entry name" value="snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04167">
        <property type="entry name" value="Snu114p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1430.10">
        <property type="entry name" value="Yeast translation eEF2 (G' domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031950">
        <property type="entry name" value="EFTUD2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044121">
        <property type="entry name" value="Snu114_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035655">
        <property type="entry name" value="U5-116kDa_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF6">
        <property type="entry name" value="116 KDA U5 SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16004">
        <property type="entry name" value="EFTUD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="135"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="994" mass="109849" checksum="4C878EFA411EFBC8" modified="2025-10-08" version="1">MADDLYDEFGNYIGPALEDSEEEDDEHHQHHAYADEEGADEDGAGPFGVDAMQEDEEPGEGGMAVVLHEDKKYYPSAEEIYGPEVETLVMEEDAQPLEVPIVAPVKVKKFETLESEPLRTHYSNEFLATLMANPELVRNVAIVGHLHHGKTTITDMFVEQTHELKKLTAAQEAAGKQLRFTDTRLDEQARAMSIKMMPMSLVMEGHSGKSYLLNLLDCPGHVNFNDEVTAAMRIADGVLLVVDAAEGIMVVTERAVRQALQEGLAITLMISKVDRLITELKLPPSDAYHKLRHTIEEVNNLIAVCCGGEVPPERLLDPVRGNVAFCAAASGWSFTLQSFARLYADISGAGFDTRELAKRLWGDLYFHPEERVFRRNPPRTTAGGGAVERSFVQFILEPLYKMYSTVIGEHPKTVEAMLGGLGVFLRTATYNLDTKPLLKQVCASVLGNCSGVVDMVVAHIPPSRKATADKVAAHYSGSLDAPAVSFMSACNPRGPLVVAVAKLFPRQDCSRFDALGRIMSGTLRPGDQVKVLGETYTPEDEEDSAVATVGTVWVYQARYRVPVARATAGNLVLIEGVDATITRTATLVADAYDEPVHIFRPLQFQTRSTVKIAAEPLNPSELPKMVEGLRKVSKSYPLCSTRVEESGEHTIFGTGEMYLDCLMKDLRELYAEVEVKVADPVVAFCETVVESSSLKCFAETPNKRNKITMIAEPLDKGLAEDIEAGNVRLEMGRKQLTEWFQSRYEWDLLAVRGLWAFGPDVQGPNVLLDDSLAAETDKGLLNAVRDSIIQGFQWGAREGPLCDEPIRNVKFKILDAAIAPEPIHRGGGQVIPTARRVCYSAFLMATPRLMEPVYYVEIQTPADCIAAIYNVLAKRRGHVTADVPKPGTPIFIVKAYLPVIESFGFETDLRYHTQGQAFCQSVFDHWQVVPGDPLDKSIALRPLEPAPIQALAREFMVKTRRRKGMSDDVSINKFFDDPMLLELAKQDADLAMIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1I1</accession>
    <name>A0ABQ5S1I1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052575">
        <property type="entry name" value="SSU_processome_comp_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695:SF11">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="154" mass="16194" checksum="451EBEE269B75B11" modified="2025-10-08" version="1" fragment="single">YYHADYVRQFAAQAVGYLLRSAPSAAALRSGVKAALAEQAAKPTPERTDGAGLVLAEAVVGVSQGLHSRAQQVLQLLLKEDLLQPSDLRPAVVSTEAESATAGTETSADATTVVVSQERLRARAAAVARVSVARLLDHLRPGKGEELWVALLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1I5</accession>
    <name>A0ABQ5S1I5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Autophagy-related protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_006615</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Preautophagosomal structure membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026849">
        <property type="entry name" value="ATG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13190">
        <property type="entry name" value="AUTOPHAGY-RELATED 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13190:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13329">
        <property type="entry name" value="ATG2_CAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="264"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="327"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="770"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1355"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1436"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1540"/>
            <end position="1594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1716"/>
            <end position="1739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2199"/>
            <end position="2224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2236"/>
            <end position="2258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2713"/>
            <end position="2749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2767"/>
            <end position="2796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2866"/>
            <end position="2897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="327"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="345"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="780"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1450"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="2202"/>
            <end position="2212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="2236"/>
            <end position="2256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="2724"/>
            <end position="2749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="2785"/>
            <end position="2796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI63605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2897" mass="296261" checksum="EFFFA09F5E41B9E1" modified="2025-10-08" version="1">MLGTTDWLLKRFLKFVLKRNVGKYLSSEIDLEQLDVKLDTGKLELKNLLLNCDAVNKDLALDGWHVKAGYVGSVNAKVPSLFSSSSQCSVVVNEVMLTVAPAPALPLPGAGSFPPGYPVPNGSAAPAPAFTPDFHPTSQAAPPVTLSSSSGGAMSFLLADDFGWTGHGAVKDGIKRIAGSLQSLLQHMRIEASNVLVRVELPHPTERGRVVTAALKLDLVEFGDLNSPRVTSPSTTACNTSMQHQHNNHRGQIYDHQAQTRNSLRPSVGSLGAGDSVNTGGPSVAGAAAATTPTSAGTTSRREIAKHIQVNGLSLDLFETTPLLTGSSTAVTSTGGSSSPLGSPNNVTASLPTAQSGSGSPPAAVTLVSGPGKHGLQLSVELRMVLPEEAGKPTQIMADLHIGAARLQLLPSHAVCLSALKEALGVQEGPQRMVDPGELGAGVEGGGRNAGTELWASSCASASASMHSAAGQWGRRSLFESIMMPNCEGVVAHSLEPYVTGTGLPYGDDSEFHDAGSRFGSLASSLVSSVTQKGTNLLGYVAPGGSAAVGGAMDWMAAAPAQVQQLSPQPPLARPSDVHLRLPEGSAVPMAAAMETAAALTAVAPADGAPLPPSAPQPIISVRAHVSHAAFVLCYGTLPSPTHTTPTHTKTTSARGCNGANLRIDCMRAVGTGAASNRVPFDGTTAATASATPPPPGTVNLRTAGSLVAELQDLSCDVMLDVAHGNVVSLQAGQLQVYEQLSAAVGASNGMSPGEWGRVPEVLPQAPHRFRPTQPAPARASGSGHSGTATSAASGVCSPKLGARQPGRNVQPTYGSATVNGGNVLQTWPILMCASTHMPSISGSVTAPQFQNLQPNAPQGAPGKAVANRNISQTAAVHPVAGAANASTSGYACCLRLCASWGGGEGTEAVGGGYDGYGSGAVSAVPSYDLSVEMLPFTLWLSMPLVQRLTEFMVPLAAEPCKAQVVAKAARPLPRLTLTVVATHVCVLLALVPASPPAVPLPRPPGSPVFLALDVVSPPHPSVESAALTSTPFHGAHHCPHTNQPQHPHQQLPYTAHYGSVPAQLYGPYGTVRHGVFGGNHQAHGASPPLHSAFSSAWGGGPSHQYGTYGYSVMHHGRWAGPGQHWASPAWVVPEPPPLLTVYQAPATDRLIPSQVTTVDLAAGVVELLIVRTASTGVRDRSQAGDAVRAAGCSAAQGHPHLWSSDGADGHGASWTSKSILCLSAAQPRASAAETSSRPTWDAPLPLPAAQTGPTSVPAGCGLHLHVCSDPGVGMAPELVPDAWGHVATHAQDRWADPDGEGGAWEERSSFRSLAFHERCRKSSATFVHLRAPKAQLHLTRTDLQDLATLLAVMQPPAPKEPPCSSTTSRHRPPPKMSSRDAAGGVAQGVCPAPQACVLLEIGLRIQLQDDHLAVRQDGLHGAVGLHVNAARAAGLHSAAPHPPPPVPSAAPAVGPAPGAAVSAASSGSNPGPSQGMGLVLGGSRVAMYELVIVELTVFRASNLCSQATCNVTSVHAHGVTLLGHLEQQHQQLQQQLQQAAAAHATSNPGGSTLNGGARGPDGRQRASPPGSGCAASHQHSTAPGFQGSVVPRKSLPGSTAPHAICLVHCPSSAAAGDSKQPSYIEYVCVEGQRPPTPQPIRGGIAHEDLGLTGAAAIHVTPSANLQQQQVGDMNAATSLHSVLLQGITVSTDHGYVTMDWAVQLGQLLEALSAPPAGGPSKVANPPRTLQPREAGRSRVKGHLSVNVIDLALRHEPMDYPTIATVPSGSNGMSAGDGGAGAAATASGGGGGRVSPVSLALIIEGIHVGLQLGSTEQEVPYTAPIHTVVLHSLTLHLAAADQRGPCWSACDPRAIREPLLQACGYHLVLQETSIKVVAKQRRYPRRSKAPVPPSDANVAAAAAAAAAATAELAVDEAEEQFHTTLDVEITNQHLLGMLTVESATLLAHFAMQAGTLMTARQAAAVAAAAAAAGAPQRRGDFSSRSVNTAPADLYWDAASENSSSVFGGGAGGGTGSCTCGYSEAGGEAEGSLLTALLLEEVEENAFRRPQLQPQPAMAAEAEGAGAAGLGGGWYPAKGCGSPAGDGTGQAPGGLILIDDFFPVNHYGEYDEDLMLVQHPPKGGAAAWRREGAKMELEGTWYGPDEGPDTRRSGNGFSLRGSGFEPEMSGDPTRAFYPGSGVFTDVDMGLGSGGLCSGGLDAGSEDPDIDDLLVPDSRDTGGDPRVTGPVADAEAAALRRAPPSVASVGSSAATRSSCAPTWRLQEDYVRAPRKSDEDGSARCGDGAGLAIGGATIGSGSRPEVGGAGAGGCATLGGGVDQPPPAFRIMFKDLSGIWVMHTMRDAGGSDGSSGSGQQVGRVELVIEGLDLLMESYGPGGHLARHMKVTVHDLEIRDCALEVKQQQAAAAAGARPLALAAVAAGAGGCSAGGVCGGGGWVRVLGYRAVRGRPRDSRAPLVALALTAVRPNPAACDPRDEEFRVAVALLPLRLKLNQDILSYLTWYGISVSTALKPAAAVLASMQAALSELDGCEPLPPPFFQRAQIRGFSVMLDYRPRRVDIGALAEGRLMELLNLVPLGGVDLVCKALTLSGVQGWDGLGAAVLREWLNDIARTQAHKFITGLAPINAVVKVGSAVAALVSAPARHLARREGAAAAGRQLAVQLRRTSATFAYRLVCEAMLAGRKAASGAGKALLRASNNALCVYGTGDQANGSNFDGEADGSSDGDRNDTEVGGNRDGDGSDSMEGGKDADECIDIDRAASLYSGWSSSGGKRTGDGGGGGGGLRRSQSASKAVRKGLSSAASNLGSASQQVTAAAELQRSVSSMARVLSAAAISGVSAAAPVSAALQGQLKGTLRSVRRALRGQLLPGGNATASGEGRPLAAPAGGYPTQPRPKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S254</accession>
    <name>A0ABQ5S254_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="710"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="942"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1170"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1250"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1337"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1536"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1604"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1706"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1907"/>
            <end position="1946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="727"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="737"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="961"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="986"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1019"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1093"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1192"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1265"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1359"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1604"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1719"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1909"/>
            <end position="1920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1921"/>
            <end position="1930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1931"/>
            <end position="1942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1990" mass="201542" checksum="81A56938F5E68D47" modified="2025-10-08" version="1">MDAGRPKRKRSELELLLGKVDLGSLSAFSPRLPALSVSSPVLAEATAESDEQLARRLHEELNGMRMTRRGSKAVTEDPASLSPPARGIPSPHGYKSKAETPKAHGGQGRSSLPLLPPITKSPPGAADGGAGVAAEDRTLETESGVPGVLGAAQGGATGTGPAAAGPGSGAGADAGSGTGFGSASVLVPPPEGGGEAVKRKRNSVPARELHLLLQQASSAAGRAEATLSPTRSARSGLLASARTEPAAMGNDSHHHSTSHQQGNIPLPPRRDSDRSNSQQGGQQHQHQPAGSTASAPVAPPSSPSVTAAPSLRGPQPPPPPSPRASLLPPPSLLPPKLKQRYLSGSSPITRIPASASASENYSGRRLEGNTTTGSGGATTVTATTATTAATPSCVLGGARDSLLSGQSVEGSSVQSQPQLHARDGSSAGAAYTLVEAGASAAASAASLVTAGPRTLAGTGASIGGCSSTDETENDNDNSCPAAVAGVGDGGPSATSPKAAPALQLPPPLPPTGPGGTSQQQHTTEAAGHTTEPGVEDDEPHGRAQWAAGTAAQPLPNEAKQGQAQGHLATGPLKVPKLPMIRAGRGWYRGRLMRESVDGHRVLLDVPGALNPVSSSSSNGHPQNQPFWLPVTSDRIWRGSYKGKDWKYLGDGAWEPKPAAFKRGKQGSSSNGLPLPLPQWAVDTTSGGAEAEAQALPVNAAAYASGVGTTATGVRSNADRGCWSSPDETSDDGEAAEEPQQVAPEGPNTRSGQRPKRRSPPTVATFGFGKAAPTATTAQVAVVAAGDLKRKGPALLGLDSPADAVAADATAGAIPEGEVAPAARRTEAKQHANAGASCQDEAVKTAADGASDGAALVTESGAFARPACVAETAVVPGKEGQAVPQAEDAVVPMEGACASIDRGPSAAVVAAAATSPRKPLIKAQREEVGGDGGEGCGSVPVTGQEEGGEDGGDIGWTPGVTHPRKHVPRRAQRRRQRIRTPFDGLQLNVSHHQPQHNPYAFFPVLSDESGRDGDASDAELGGGRRGSGDGTVTAPQHGSRRRAAARAAAVAAAAARAEAEAEEEELEEILLGRRPPRRDRFHPQHGSGGVPGRRNPHNQYHHRSRSGPRADSYLYGDSSGNPDDPYSVDGAVYVHGSSIRPRASSRGNPQPAPEYMSVASESWEALPWQTAGLNQMGDGGTGQLPSAPQAPPRNRRKGKPSRSRMDGEGDGWCCGEDDWELEARGGAWARMPEVLRRLYYMPCPAVAVANADGGDTGNSDDAGANLQQQQQQRQQQQKLRQQQELVEAGLALTYRQSGGGAYFGALQFSNKRRRAYEDDPAVSLDDLDLDLEAEVDDHEHDADYLPPYGTAGGSRRSSSFGGGGGRRCSANGGSGGAVKRQRAGDALYLNDHPSHYQQCHQNGHDSEAAMVVLAHPKRRILVTCGLPPGRSSGTLGSAGGVTAEGVPGSGTVDGGIPAGLAGLLEAALGQDTASAAAVVHVANGNDPTGWVPAVKVELEPTGLDTAPAAGGVVDRLAGGGAHAAGATGALPPLEAGSLPALAPGDPHGAHVALSDSSAAPSGLEDSKYSGGGGGSAAATAIDASGLVTCVDGSTGDAALLLQQQQPQRGSGTNNSSGGGAGYIAGGSSPSPPGVQFDRLRSNTPTRAKRTGRPPFVGLGAGAPMPKLPALSSTASHRMQMVVMAEAAGLATIAADAATPIDGLAANGGGGAAAAAAEAPEDSHHQPSTEVLDGRGSEEAQVPTRPAVDVTMERPMGVKSILPADSELVEEAHKTGCGRGGLAGACKLWQTAGGGNGGGGAAAKAMQARAVARDVGGGGATCQRDGPVLISNTHIGAASMPATKPYGGGEVTGYCVMDAIGALKFDKQPAMQQLAVRAQLEVEMCAAQHHHHHGVAARTQDEQLLQVPQHLAQQQQRLLQGRGSSGRSGGSGVARSGSLSAAASPTASDPLISSLPSVEVVVNLYSARTPHGSAQQALTELVGGSTALPLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S354</accession>
    <name>A0ABQ5S354_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007492</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="467"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="558"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="749"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="909"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="988"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="789"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="250"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="579"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="603"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="623"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="658"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="772"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1011"/>
            <end position="1020"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1177" mass="117392" checksum="C5C104E6BE267A85" modified="2025-10-08" version="1">MKARSTGSGGFRAAYYTEEDFMYNDDDIDYIPPARKKTAPIGGGGKERPGFPKRPAAQSPPPGAGSQSVSPSKAVRSRPTNNQLLYKAYFRWAHYPHKLKNANLPDETDACASVTAAFLQVLQIMQTAEQLRHGNNKRNVLQILSSNTRRTKVVELSSGTHRVPDELKKHYLSDGEGGQYSTNLDRNRYGNDDNYLFTVIRYDLEFLGLCPCMVDVEIIDAKQGNTSIIVSAGTEVCTCQGGPPPGSGPVGAPVQGGSTAAAAAHHHNAAPGGTHMHHMPAATLSGGGHAAAKAEYSGSMGPHAHATGAATTAGGSGGYAGGGTAAGFGQHDVGNLQPLFGSGAFRSGPQLGVGRESPKRSGNSVSQLFDALVMAATGGADGAAAAGGMGGEPPVTSPAHVDRPERAVGGGGTGGMGGFGGTRLHGPPGRNKSKLWSLNNLSYGGEVDSLQNALDAFREQDEIASGDPAAFLTGGTSAGGLGPDGSLQRSDSLRQRRRKPLRNSSILGRPGSSGGVAPLSSKLGINLREKSDDNLKLTADPDLGGALTGGGLGGGASFHGGGGMQLGHGPTQVSSLVSGEDDNDGGAGEADADDTLAAKGEDLGVEGEGEAGVEEELQEDGAGDGCIGRAGGGAGGGIGPTASGTVPRGSALPPGRASLRHHVRHVPRQSSVANLGPRAANSSGGGGGGGNAGGGGAAGSGLGPLGLGMLGPGGLGGSASAAIAGEQLRRLANELEVMRHENRTLRQQLQRANSSNVNGGGGGSGDSPSHGKQQQQSLDTHTNNDSARLRKLEAELLSMRNELLGLSTAKKSTDEENKQLKRELLASQQHVAALLSRMLGAAGVAGAGGLLGSALSPVSASPSAGLLASLLGNSNTSPSPAPPTQASLIAGAFDPTYFAAHLVALRQSVPPQRHPSSGGSGEGATVNDGRSAEFSGAGGPVEALLAATAGIMGGRRSGAGADVMGLEHGAAAQARKRTADEAGLATAVPAATADRGGGDASTTLEAEPLSRRGGSAAGAAPSTSIHHGLIGAAAAAAAAVSAGKQVTSQQQLQQLTSLPSFAPAIPASATSFSMFPRTADSGPAGTPADSNCFSDVAQLLALPNLDSGFTSSELGAAFGGGGCSAGVASAALAATSGVQMLGSASGSGTASVPQMPQPEHQMLMSAHYSLQHPSSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3H5</accession>
    <name>A0ABQ5S3H5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009818">
        <property type="entry name" value="PAM2_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07145">
        <property type="entry name" value="PAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="114" mass="12860" checksum="DA8B8CAEDF4292A4" modified="2025-10-08" version="1">MASKQLNVNAPEFIPGSWKSHTSQLYNGKNNFYYYADTAVEEAVTSEELEELEAAEDWVRQMAELDEMEREHLIATALSEAEPSQILEVEMRALALDTAASGKRKAESKRKGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3N0</accession>
    <name>A0ABQ5S3N0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039586">
        <property type="entry name" value="CFAP46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15977">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15977:SF15">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="729"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="815"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="760"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="820"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1141" mass="118691" checksum="1725722A38158D09" modified="2025-10-08" version="1" fragment="single">KEPGDFEAGGAGGAASGTAALTPLPEWLEEAARDLGNTDLVTPGSSGLLGHVTGQLLLRPYSLHDVWLKKGEFLASRGHYAAARKLLTRARAHAADCDNREAEARCLMALGRAELAAANPGEAVKLVQSAQRLGGDINFWAELLVQYVDCRRASRDSTSAYAREALQGGIAMFLALARDDRSAEKPASAAGAVLRVRLARLLLADMEMLRSQGVSTWRKSYDQAVTLVNQAIMALAAREAGIPHIEALLMQAELMRAEPAPVKDLRPRLKKVEKVLLAAEEMAVRAQTECTPRDLDPRVVTPTARLLARVRCELADVQLAAASERERLAAEDREKGRPKFPRIRGKDVKVVVDFIDETGGGGPPAPRLLPEDSALALASGAAAIVAAAPRDRARALLVAGRCLQFKFFASVPAPLSPMRTFTPPPPPPAAAAALSPPPGDVNSGEAADADTSAASAADPYAAAAAAAEAAAQTEEGAAALRLQAQAAATLAAALEAAVSVQDWQLAEGAAYELALCYGQQAPSLACRSLAVAQACRAAAAGQALLRAAAPPQQPEVLALAQRDALAATLPAPIESPHYAALRKLLVGLKGTAARLDVAAAPSVERQLLSLPQDLRVLCLYFSPDGTKLFAAALNIPDPLGQPPPQSEKNRKKGDAAVAGAAVAGGLGGGAQAVPRICLLQTVDVNKASADALKLEFRTYRRGVERQIREGISAAAARGIGVVSEEVRAVSPKKSVKGQGSAAAGKRPASKQQTSKPATSPVGPGAQQQGAEPQARVDIFDPAINQSWMKLLDQFECWMAPLQSWLEAAVPAVPAAPQPAEGKKGGGKDKGASSGPPKHKVVLLLDTCLDGLPWEALQYLTQNCTWVGRAPSLQALASCYSTGTSDSGSAAATATDVAPPTLPPFDLGRVTSIIDPRYECSTPAQARGQYMVPLIPAMGAPELVSALPAVQWWSGFSGEPGRAPSPDLYAGLLAGSSSVMFLGVGRFAAHVAPAVLASADLSACDVALLFDRCNTDEAFWAQLYRDNRKSAEQRRLESPGRVAALLLARGVRTVVVTAAAAPPAVTVKLMSGVLAGLAAGRSVGEAVYGMLTGPHLDEFELMHLKANLQVWGAPGLIGQVITQGTKGGGKGGAPPRGAAIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3W7</accession>
    <name>A0ABQ5S3W7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Molecular chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007759</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="498"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="542"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="61889" checksum="E8CF234525C4B9C1" modified="2025-10-08" version="1" fragment="single">MHQQTRNLSQSSRPCGVHHSWPQPHVRRSCQRRALAVHAKLRDFQREVVVGIDLGTTNSAVAYMEGGKPNCIPNADGETITPSVVSVLRNGEVVVGRRAQRQAVLHPLTTYYSAKRLIGRQYNDPTVQEEITRLPYEVSCDEAGSVVFKCPRVGPGYLYPEEVSAQVLSQLISDAAAYTRGRVTKAVIAVPAYFDDRQREATIAAGKLAGLETVRLLREPVAAALAYGLDLRTDSVVLVFDLGGGTYDVSLLEVGNGTVEVLSTGGDAHLGGDDWDAAIANWLERTHLRPAGLDPATDPRLRANLRALAQAAKHSLTDSEEVVMRMPVGGPGSGPLEVRLTRTQLDDLTQELWRRCRLPLDQACWQAGVDLNEVVGGHEALKEQLRNRGVPGWKIQALQPEIRPRKRSPLGTVLLVGGATRMPAVGRFISNMTGLQPLEAALDPDEAVALGAAVQAGILQGEISNLMVMDQWQASLMRALAKLQLQADPRVRQRLEQQYSLEDEGESIVDGEEYGEEDKGEGSSRTGGRKAAAAAAVGPKRPLSKRQRRRQQQQQQRQEQQREGVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S402</accession>
    <name>A0ABQ5S402_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_007516</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF118">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="49"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="321"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI64295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="779" mass="84688" checksum="90EDE690A739F935" modified="2025-10-08" version="1">MLDIEDGPSPKKHRRNSKAVDAVAAAERRKQIDAERRQLPAWAARDKLLQLLQEHRTLVLVGETGSGKTTQIPQLLLSARFGSIAASDKFISQNAGEVGPSDDVKSPYEGAGVVDRDGVFRSAVNHEGRTQFNHHTSTGSDVNGVSDHGGPEQQTGIRSVGGSGGSRVYGGAIAVTQPRRVAAMTVARRVAEEMGTKLGDKVGYAIRFEDVTSSSTRIKYMTDGLLLREALVDPMLSRYRVVIIDEAHERTVHTDVLFGLLKGVQVRRGDDFRLIVMSATLDTARFVDYFPGAVAALIRGRQFPVRVMYTAKPEDNYLDAAINATLQVHSDEGEGDILVFLTGQDEIDSAERLLKEHLAALQGSRGASSSGCRELLVLPIYAALPPEQQMKVFEPAPEGQRKAILATNIAETSITIPGVRYVIDTGHVKARDYNAKLGLESLAVVPVSQAQARQRSGRAGREGPGKAFRLYTEADFAVLEPTTPPEITRCNLGSVVLQLKAMGIHDVLGFDFMDPPPRAAILRSLELLYALGALDATGCLTEEVGRRLSRLPVDPMFGRVLLAATEMGCGQEAVAVVAMVSTENVFHTPRQKEREWRAARLKFISREGDHLTLLNVFRGFRELPKESNKARTAWCSDNFINIRAMRKAEDIYEQLVRFLGPPSPPGLGLQLATCGEDSTPLRRALTAGLFPHAAKLQPDGSYRVLATGRQVFLHPSSVLLDRKPDCLVFNELMHTTRTYARDAVAIEARWLPELAPAAFAVKSAAAIAGAGVGTSGGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S444</accession>
    <name>A0ABQ5S444_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_007396</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF758">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="1700"/>
            <end position="2148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="230"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="552"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="612"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="736"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="869"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="926"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1074"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1233"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1314"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1548"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1764"/>
            <end position="1829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="325"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="366"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="555"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="635"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="736"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="869"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="890"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1089"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1234"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1326"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1349"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1560"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1577"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1599"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1802"/>
            <end position="1813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI64204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2153" mass="218081" checksum="487978A124FD7463" modified="2025-10-08" version="1">MSGGEGDLSPAHWRQASRGGRQASGRSGSSNANYYRSQQHYRDHQYDDLRQQSNYQGERGFSHRGQQHGRSYGSFAQQQDGISVQHEDQRHHAYSRHNPIDHPHAGFRGQPMGPRFNPAEGRQPGYMHYPPESMLAVPPRAPEHWQSGPVPSSYQHPSVSSEGIHAQYGRPTAFVDQPQYPLPQPFPQHQVAGGHLGAGIGMCAGGPVGGSVAMPGPVYHPSGLRLPHHGGAWTSGAPAPEHRTSEAFRGGGSVPGRGAGGRGSSGQSRGGIRDGGVANSGGVGSISARRTSRAPPVPQPLVQNASPQGEPVNSGILSLGRPRQQPLNTVNNVQSVRGATSDAAQTLSSTNNSKSERVPADAANKGPAESGGEPAPAAETAALAAGVEVELGIQDKVDEVPPMPLAADEEVHMSFPTGPLGPASSEAVPPAGLDTMIESASGCTESGIYLSNPSTTAGSPPACAAVNNIGTLRATGSIHPQEQERQALVEGPNVAQALQNSNPLPQASSPLEAMPGSVQAAGEKPVVPGSAWRQRPATALFASPAAGGHAAIRKANAPAAHSATAPQGGAGPGATTAMTGMQPTAIASTVSRNSTGPHQNNAPAGRAIAAMGPEQSCVPPPRVQNRPSPIQTGPPQHPRCPPPGFGAAPLASVSPVGAATVPGDQRPVVQQPENAHLSQSPQQQLSPRVSAGSEALGAAVSPARTAQVAQHSSAAEASELSEGMASRKWASVAAKAASSGGAHSKAPIRRPISEGGTSDASTAVAAATLVGLRHAAHQPPGTASGVSPGSTIVPAVAAAASPPFSMGVVDSIQASNATAEVASSISHPVACADGTAIKEPVAPMALGGGTAPQQGHAALSSKAVLQPGPSNVASISTPLQSKPPSGLPSGGPVASAAVKGAAASTTSTPGHRSWVDAAIASPANIKRAPMGPGAGPKQAPTVLGNDTSCSQSAMVSSPVADAATSAAANEPAADANVVEALPGAAAVASGQGVEDTNADILEAETRIRTWEWVATNGGGNSTPAEIPALLLEQESVKPVAVTVDSTTELPEADPRAVEPAAPAEVPAAQAKQSWAAMAAARPPQLTGHAAAQAAAPKRSAAFQGRGLTARERHVQNPNRGLSAQPPEGMFYPPAAAPVPPTAGALTPKKASEDGHGASRPSPTPSHMPSGAVSVKLTVDADTASTAAAVTPTTHTVGTADLSTASAVAASASDFEASATAGAQPASSVDVAQQGVKSGVSGSHGGPAASSASAKGNVTAAAVPGMPRQTDALHERRSSITGSEPGAVKTESAGHQADGDDTGFQLVVPRHKAGKFSRGTDAGHSHPGPSTGTAGASAGRGRGRYGDVTAAAAAPQQRGAGAHGHLALQGGRGKGAAGHPLQQSPQQFAAGGKHGATAPADMSMGPVATIPGASSSPIVQPASTTNSTGSAVLGVLADAQANANGASVPLAVLEPLAVPAVSEADLAARKEAAAVVPTVRQDDTAIPTVVVTLPVAIAASAASFESLAAQENVAAQGITAIVVSPNEAMTPVPEDMLTVGKAEASVAALPPPDDAAKKTPVQRTQSQARGPNTKKKDKKDSTEAVPRAAEREQEAKPLEEGEEDDDDDDGSAAIPTGSNPWDLLGAAGEEDGAATAAAEVPAVAGAAAGNGVASLAISVASSSEASLVEALRQLLTPATAEQLLAASQIQHQAGGFRLQPRGLTNTGNTCFINATMQALLACAPFAFVLSRLPTIAPLLEPSKSPALHGLALFLGELVSPQSGAAGFASAGSMPGGREDAQQAAEDEGWAEVLSHRGKKGQRKGTSTNAPQQPASPMVPAPATSSSTVTKGEAPGSVAVVLGGQPLVPSMLNGIVAAFNPRISIAERQTMTKVLSKGVIEQEQQDAQEFFLFLVNQVHEEVVALRKAHGLATEQAATAGTGADIAGADGEEWAQVGRKNKATVTRQVGAPPDDAASRSPVTAIFSGLVKSTVRFQQPNYKPSATIEAFNMLHLDIAAEGVNTLEDALRQHSVPESIEYKPDGATEILLAKKDAKLYRLPEVLVLHLKRFTYTAAGGGRYTKVQKPVTFNCTLRLEGKILAEDCPDRANSEYRLFATINHHGRTIAGGHYMTDVLQPDNRWLRFNDAEVYVVTESAVLGERPYLLFYQRVPQRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4G4</accession>
    <name>A0ABQ5S4G4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="411"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="544" mass="56015" checksum="D098C7552E87823F" modified="2025-10-08" version="1">MAATADSLQREVEALQACCPALCPAIVPNGYDTKDLVGLIEHAGKESLNDFENLLDLGGEKVTACCVCGADGNPSASGSESEAEDEDDAKQTLSFTLLTGVDFKTRNIVLRRAGFACMQCRALRSTCRMIRFAALRVGSRGEGSSHSDMDADADTDNSRQRLLLLAAHLAAVNKAPVSIQANPDALIVWLQELYCRAYALQVVASNLSGWRAVGPGGQPLRLREGKDVVAAAKLLLGDLTSEARAQNQKQQNQKQQKKKRGNAGTTPRQVLEIRSADTCAPAGGVGKGHGGLHAAQPPGQAQAKGKGKKRTGGDASLSGPEAGVSGAEAMEAHYDHRAAAAAGELQAAKKRKKAVATAAAAAAAATISGREAHLAKGKEAAIPGAKASPGGSRQLSGTTLEAKNVTTPSGASKAAAAATVQRQKATKAKKIKQHSKGLEASDDGLEEGHGIDKEGDGAKKGAEGQAGRGKQHAGFGAVAAEQKLPGEGKVARAQAAAEAGERRRRTRKTAPGLAVTEGKEVGKGAPSSAGAVERQTKKPKVSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S579</accession>
    <name>A0ABQ5S579_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007752</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="2">
        <location>
            <begin position="194"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="623"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="642"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="670"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="729"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="746" mass="80502" checksum="3C3DCBAAE4E84E0E" modified="2025-10-08" version="1">MVLRLYCSNLALVCAKIGLLCVLTVLTAARSERYITAVGTVAIMSAFGRPPPPDEKPLSPASTLDESPADNWHVATTVNTDLVELRCPELRNPATGFLMSDPNCKVPLRRLMRGSQAVAAFQRENKVLTGDKFKAVFDIIDKNTVKAPTLNSTRRQLPATTVGIPQLRPGSFRLLERGDQKEMYTGAPIQLRAVVYLLDFCGWKNPFRSAEEFRKYIFNEPGSVEGNLQNFYQTCSFNKTLWDPSSVVVLGPFTVDCKGVYIRGIFSTAFDASTRCGPGEQASWVYAAESQAQKVATKGSVLEAVLQYPTRRRVLVVLPAEAKCGFAGLADVACSSITCRSYIKGTSAAEVSVLFHELQHNIGLSHAHRGFEEYGDPSDPMGMSMRGSQAVRCHNAPYNWRIGWATTVPNGNLTAGNFTLASNRIRLTIPGSGFTDANMVIVNLGRAAHEAPLRSIQYFLSYRVRNETLGGYDRGLPSSYSQKVLIHTYDGTFSERDFNRSDFIDAGPRFLQKDPAFPAGDVWTGPFTPFNSTSGLGGGLRIKVVSVGPSSAVVEVCRMYSQTEGKPGSAECDSNLDRDCDGLLAAVDPDCIGTWDSDSSTSQRPASQLPGAMEGQAGQAVSSPPPARSPPSSPSPVRSTRPPSPRSPPPRSRSPPPRAVGLGRLGPSHSPHPPVPPPSPSPQAMALPLSHPSPTPPSPIPQPSPPRTQPSSLPQPPRPMLPSSLPRPQRSPPSSTAPPLPRFPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5M4</accession>
    <name>A0ABQ5S5M4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008686</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="418" mass="44759" checksum="A6B32BD15D1AF268" modified="2025-10-08" version="1">MTSVPQTTQGATRVLGSGRGWAPGLPLLSTLALNNSVRLPNVFTDLSKAWPAPGAGRAVRLKTRLKARATKDNNAGSNSGSNPGDEDDELDRLLGTGRYRDDNTGFFSMRNRGDASVIGEGEVPAGSSSEAGDTLADLMQMEVRRVRAKEELVSDLEDRKAQMRAIGEEMKAALAREYELDRLRTEIGANYAMSEAFHALDEVEAEVQRIKQQLQADKADLAAWEAEAAEERSKGLFFKNLYAIEKEPRQPVGKSIPGYWRKQRAAAAAAAAAAAEATAAGSHETSPSLLGRSGSSSPSAGGDSRWDSDAVAAAAERPLSAASHQVRSPLRLYLFSYMSAFLALLVINDVASSSLAADAVKEAGLHVAQVALYAVLSAGLGFVALRERQALAQAQEAMLEDIRRTAESRSKTQQEKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S612</accession>
    <name>A0ABQ5S612_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008579</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="257"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="550"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1104"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1284"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1318"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="460"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1107"/>
            <end position="1210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1530" mass="159383" checksum="91D29869E24E7BD3" modified="2025-10-08" version="1">MPFLRHDSRTRVVPTDLYAVSAAADEGRDGTDETQQQQQQQQQGRSSRAAVNDGDNAGRKSSYSAVAPPAAAPAAAADAPAAAAAPAAPAAKPASTTSAATAVPATAAPATPAVPATAAPATPAVPATAAPATPAVSAPAAPATPASPVTAATPTASPAITVIAPPDAPASPGSGAGFGGSGGAGGNINEATDSGGSGNHHHHHHHHPTPVPRGAGGDGGDGGAPSAAGAGRGGNTKEPHADHDVKVAKVKAMLKNPKSTKPADVLRELDKNGDGKLNMEEVADLLDLVVGLREQSAFFKTMVIVQAIALLILLLALAGMAAAIVYFAKDTKISGSTLVNKHTGEPVLVYSADFFVTSSGVLVSRDLSSTSSTAGATSSTAGLLITNAAAQPPPPFSRPPPPANSAATPSPPPGPSSSGGRSRRSLFEELMYPDERDQAGAGGNTLSSSLSSHMHLRHVGANNTNNGAAGRNDQQQQPDLQQQRGSTSSSESATTAAYGEDHLTSSSSLASTGPFDDGGDGDDGTVKSPILKRSKVRNFFRSRGLQSLLQQQQALPPPQQQKTLPNPPPAPVSPSPSSPSVPPGASSTPPPSSPSAPPSPPPPRPPPPHPATPPVGTLTCTGATAAADDRCTDANSSTSSTGTANATSEFLAALGGGVPQGGAPLSTAASTVPMGLHSALPMSVLKELKHIQINTSTTGAEVNLLVLAVAKMPAPLEQGAYASCEVVRIVTYVGSIILHGSMLEFGQGSSVGDIFQDAGFSVTGGGPRRTLNGTYGVIGFFNIIKGVEITNLPPSDPKPTIAPIYQMKMRIYDQCYSEGTTSSSDDVGVDSCVDPDTGLDRPGTVMVQGVRFMTTLQTVFRQHDLVLTTSEFPNHGPNLIRKELVNETSGDVFVWQDIMQGTRVTHCEFLDGAAEERVMFRSLSRDAIASFTFLGYEMKLGVRARHFRLVTRQPILREKNASESSINRGALYDVKLVDMDLWDTADNGELCSIVRNNYPVAFEMVHPVGGFTKIHVISFQAVITQPTPVFALPSNFDDTNSPMGCPGPRFTKVPIMTSPYGPIWQPPPVDQLDSLFSLYTKLTADTNSESDDAFLGLMMASNQSYPPELPPPSIPPSPPSPEPFLPPSPPPFPPGSPSPPSPLPPSPAPKPPPSPPFPPSPPRPPRPPPSPPPPSPPPPVPSPPPHPLPSTPSLPPPPTPPPSPPSPPSGSPGASSGNSNSTRRRILASSIMDFMSSSSPDGSVIEAMVPIEEFNEMLKPVLRSARLNGHGDVIQELLGVSSSSSVSQRSTVLHRAGDESNNPNLDLDLDRDEDVVGNNTQNKQEQKQQQQGLRGSSRNLLRRPRNYTLSEVCGVSFSSDLLDTNFIYSIDCGAPLSWLPSVEVHGSIAVQLILIRQMNIVTMRTCQVSGCLNISIDVHELLGTTIGASKALGWLTTHGVPSSVSLDGACAAYDRVDRVGVMSSSWKLPNSLLTWAQWQARLETNFESCFALNTASVVIQYRSWCGWSFCTKTLYEVQLTRGSSCPPHGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S637</accession>
    <name>A0ABQ5S637_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_008923</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03467">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012748">
        <property type="entry name" value="Rieske-like_NirD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43456">
        <property type="entry name" value="RIESKE (2FE-2S) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43456:SF2">
        <property type="entry name" value="RIESKE (2FE-2S) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13806">
        <property type="entry name" value="Rieske_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="9">
        <location>
            <begin position="81"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI65372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="311" mass="33853" checksum="871EDEAE178534F7" modified="2025-10-08" version="1">MQQMQTQRIASSRAWGCVTPHVVPLGLGRRCALAFLSVQCSNRRQLACQRPSTRVRATEESVEVKPLASTSAPAEDSDKWVPVCRPEDLPKGVRKEVEVDGRQVLLFWYRNQIYCIEGRSPAEGAYSEGFIKAKFTQDYCIECPSTGSLFSLKDGSIVSWYPQNPVLRVLTPAAYCRPMEIYPVKLTQEAILVDVENGSLGGLRSARGRGGAGTSMENNNVFTVQPTVYFEGMDPTRESASVYADSSAGEAFNPVVLITGIVAVGMTAVAGTAFAIYYENLTALIAFWVVFGLAVGAAGFSYVNKQFKGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6D9</accession>
    <name>A0ABQ5S6D9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008305</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046809858" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="43"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="487"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="642" mass="67354" checksum="621F7B7991553F27" modified="2025-10-08" version="1">MRHLFMLVVVVLCATTTSAYNERNYHRTLLERTTLNSNFRELFPPYQCTRDISQSRFRIDPIYSVSGSTVCFTVRVVPCGIGGGACCGNDVTFSRLELSVGLPCRRSISQVTVNGMPAAEQADFVWYGVAGDKALYRLGGLGLRASDADGKQICFRLGGTCPTMQDLCGEADGLCLYTAVDDSNACCPVGLLGNLPVATASLWSILFQTPPPPPPSPKQPSTPFPRPPSALSPPFPPPPHPPPPSPSPPPPMPSPQPPSPLPPLRPPRASSPPSPPPPSPTPPSPPPPSPIPPSPPPPSPPPPSPPPPSPLPPSPPPPSPQPSSPSASSPSSTSNYATEILTSTPSGLSSSVRLSTITSPLSPPVPTPPPPTNPSPPITLRRPSLSPQPSSFISVLSSSSPPPPPSPPPPPPPPPPPPPPPPSPPPPPPPPPPPPPPPPPSPSPPPSPSPPSHRAPKPPPPSPPSIPLKGPSLMKASTSTPTVSTSFPFCKCNRTARLMPFTLGPIATVSRNGINLRYCLNLTTTSCADPSSKCCNQNIKKIEWWTRDTCRGSVRAVYLGGAKVNQQWGPKVFKLPTLDVSRAAVPAQGLQLCIEIATSSSCSSLSSFCFRGDRGQCTYAMFSADQKCCPISTYAAVDSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6J7</accession>
    <name>A0ABQ5S6J7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Neurochondrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008709">
        <property type="entry name" value="Neurochondrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13109">
        <property type="entry name" value="NEUROCHONDRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13109:SF7">
        <property type="entry name" value="NEUROCHONDRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05536">
        <property type="entry name" value="Neurochondrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1215" mass="125118" checksum="4D0E8846E8CD7F90" modified="2025-10-08" version="1">MEISAEANDTIGFGKRNKFVPAIPSAGENGPECSQDFQQCLELLRGPGDERRFVGLLLVTRLLPHGSDDAVRKVLEALGAQFLHRLLLPLRKPAQGPQVSAEAKEQQATGCSLALAILAAGCRLHDFAAGPDVRDLLPVLLKVVQHGGVSRILQLSTAPADGGVRDAAAVSDALECLLGAAAAAPEQTLTILERSPLMESLASWLTTAAAALTSAPPGASGIMPTDEAQTHGSVLLAAALLERALTGGSGSRGKGGGRDGGGRGQGIVVFAEHSEASAHVMVALATLLGNPRLLDPLPAVTQGSVIASQAAQGPQSSSHAEGNPHPVIRGTEASDGSSAAATAATGSRGVSGGAAQLQLEALHLLLLMLRACQEPAAAAAARELVRLDEQVAWSAAVRRGLGWLLRSRAPGTLKHLALQMAAVVAETVGDGWLLGSRAAAAAAALDSEAPPRVPPPLLQQQQQQPPMAQEEPGSFLLLLVETLKIETGLLLQDALHPDRHVPATISHRSSTLRHAAPPTPSQQLLDELAARAAQRKAAEEAAARAAVGVSIVGGGAVPGPASSAKPLPPLAAAATEATTEMEVDGASVFVELPSDMQEGRGVLPDSAAAANSSADSAMMPAGQRALLLLPAAFRLLEGSVEAVAADAAAADEYMEAEFSEPTSSARSSPGHRQPVLSDAALQKLMSAFNAIIETLLQFFENAHEQQQNQQLEAAPATVTGTSTLASAATGAVPSAVLLGAARVFGRYLAEAPDAVRSAPVLQALPFVMGLRSGHQEGRACNDDDDNNDDNDDMGNFALTFLLPGLLLATGPGADGRFETTRALRTSPSALSACVAYLAHCVRGAVAATPLAERLLKEAEGGGSGSGGGGGGCAAALEALVRFERGMADVSMLLLNILEPGNVLSGLPAARHREAAEGHYGDNTTAATAATAAETWKVLEHSFAMRGRHADAATSINAAAAAAAAAAPQHLPPLLVQLLPICWLMEQWAWARENALRDLSFLDPLRPSSAVHVLFRWQLGTRALLCAACLPVVVLLSNITSLAATAASCGHGSALRQNQQRKDADANAHPDTDTDAEVVKVTACLLPQSIVERVVTAALAAAAGGTSLQLAAQLPPPPGFTRNPLLPMTPEGLADDVRGDGEVCWERLLAACVQLLEVSPPVRCLMALVSGRGASWLEQLLGAHARREPAAEELLLSEVNLARLLQLVLGASTTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6L8</accession>
    <name>A0ABQ5S6L8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009036</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026171">
        <property type="entry name" value="FANCI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029312">
        <property type="entry name" value="FANCI_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029315">
        <property type="entry name" value="FANCI_S2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21818">
        <property type="entry name" value="BC025462 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21818:SF0">
        <property type="entry name" value="FANCONI ANEMIA GROUP I PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14680">
        <property type="entry name" value="FANCI_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14676">
        <property type="entry name" value="FANCI_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FANCI solenoid 2" evidence="2">
        <location>
            <begin position="24"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI helical" evidence="3">
        <location>
            <begin position="140"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="444"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="559" mass="57790" checksum="B11D6A9F0AFBA99C" modified="2025-10-08" version="1" fragment="single">LGGLGGLVGGFGGLGAAGQEQALPAVVLLSNLAARYPAFLYEHATKLKDAFQSFLLLPAPVALGLLLGLWPLARCRRDLADHMVMLLRKVMFSREASSRLVAVRGFLFILVQQLRAAAGGGVAKDEEQLRAAAAAAMPSFSQPSCSQSSLSQMQALASGEGVSLQHELQGFLRRALGQQAAVRAALYDGLSEILLVDPGSRDVVLELVAPQFYRYVTEGVTMPPLYYKLLVAGGAGGSDPVVLLEPLPALLACVRRLVALCRDPWVGDGDGGAVNRDGRGPYGLGLLADEDAADEDVVMVEDADDEHGGHVQGTDIAVQSLVRSWDSFTRRLLRCEAEHFDLNCSGDLNPATANGAARQLQASCLLGAVEVVMEELVGIATGPHASETAGRTGERLSRAFELHSRVYDLARDAKAPGGSRTKQAATELAAGSGGDGSGGGAAGNGTAQQGAGSAVAAAGGGGASKRPRDLCPDLVPVDLRPPAMSPRCLANLCRGVLRDGLAAEANAAHTDNAGGPSAHTRLARSTIFQAFTLRSCLNCLRAASSATGSALSDALVECA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6T8</accession>
    <name>A0ABQ5S6T8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="125" mass="12323" checksum="1D8F286D740E1A34" modified="2025-10-08" version="1" fragment="single">PSPPSPPSPLPPSPPPPSPPSPFPPSPLPPPPPPSPPSPFPPSPLPPSPPLPPSPEPPSPMPPSPSPPPPPSPFPPSPPPPPPPSPLPPSPPPSPPPSPQPPSPPSPDPPSPSPPRPPSPEPPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7C0</accession>
    <name>A0ABQ5S7C0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial cardiolipin hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_009423</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09171">
        <property type="entry name" value="PLDc_vPLD6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051406">
        <property type="entry name" value="PLD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856">
        <property type="entry name" value="CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856:SF1">
        <property type="entry name" value="MITOCHONDRIAL CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI65802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="218" mass="24117" checksum="DE30DCBEFDF4339D" modified="2025-10-08" version="1">MGCCFSSPADKYATYKDKQVEDLKAQVDQLQKALIVAKGSGGHATPVKGGVVETLFFPDAKLPCRNMNKPGGCRRKNCEYAHEATSLTRFLDYLGTATRTLDICVFTITNDDISDVVLNLYQKGVKVRIITDNDQSSTQGSDIAKFRAAGIPVRQDHSPAHMHHKFAIIDGRLLLNGSFNWTRQAVTGNNENVTVLSDLSLIQAFQAQFNKLWSMFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7K7</accession>
    <name>A0ABQ5S7K7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="103" mass="11081" checksum="5FB5EE901E3462E9" modified="2025-10-08" version="1">MISTFSNVWERQFLPNILGCALGTVCLIFGGSSPPKDVAVRRFLLFTRALKSDVLVQTVIVQQLVVTHATPLGVLLVKATTVQQFEATPEACLNASVPVRKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8E0</accession>
    <name>A0ABQ5S8E0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009216</name>
        <name evidence="3" type="ORF">VaNZ11_009217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="128" mass="14241" checksum="DED424536994DEA7" modified="2025-10-08" version="1">MVLATNAARIHQKEEIQAARRRQAKSPQPSKARNSSRNATNSSKAAAKKVTNDGDVRPSTKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRRKKVKSASEDVRCTRARRQRGSSGSKKKQVTKCPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S936</accession>
    <name>A0ABQ5S936_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">VaNZ11_010228</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF127">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C AND CYCLIC NUCLEOTIDE-BINDING_KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="17">
        <location>
            <begin position="67"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="507"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="632"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="779"/>
            <end position="1041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GLI66430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1090" mass="119407" checksum="BC906440DEA55DBA" modified="2025-10-08" version="1">MGCASSKDIGPGPPGKEYTDLNNAYDAGVLSVYLTGGPLTAKDYKERLVSSEGTQTVFMPQSGYTIRYAFVSQRGYYPDAPNKANQDSFCVQTYFGGDAEQCFFGVFDGHGEYGTQCSQFAKEKVPENLLNNTHFAVSPEIAYHQAMVLSNNQLHRSEVDDSMSGTTAITVLVRGRTAYVANVGDSRAVLGERHGDRVLAQDLSYDQTPFRRDECERVKRCGARVLTLDQLEGIKDPNVESWGTEEEDDGDPPRLWAPNATYPGTAFTRSIGDSAAERIGVFAEPEVVTKQLSAQHPFIVIASDGVFEFLSSQSVVDMVTKFEDPQEACLSVVAESYRLWLQHETRTDDITMIVIQFQGLEDEAPQMALPTPSGAALDYSTRAVPSMAGMALRRLPTTRLAAIEGPGVSLEDLQSELRLLQQKKAAAAAVQQHQQQHQAMAAAAAAVVAMQPTADQVSAAAAAGSSLSLIVAAEGGSGEGDAEAQSPSWRTPDEGRLLSFATTTNFLFTSMSEPERSDVFMLFERYPVKAGDVVVRQAEPGDYFYVVESGQYDVFVQSGMDPPLLVHTYSSASGQPASFGELALLYNKPRAATVMARTEGLLYRLHRSAFRSVVAAGGQPSATVRTLRGVEVLQCLSLTQLQRLAELMDEVTFEDGSYVIRQGEEGRDFFVISGGEVSCTVKKHLANPDEQAKEVLRLYAGQYFGERALLTSAKRAANVVASGRVTLLSISRAKFESVFGPLQDIIDAESFWKEAVAMQREVLSRKTMASGLQVAQAQFSLEELSARGLLASTDCSALMLMEHKASEEVYTVRVTSVTDVVALNKQAVVMRSRDVTRSLEPSFFVPGVLKSFKDNRVLAEVLMTVGLCTLDCLMVPLPFDEASAMYIAASVVLGLEHLHWSGVIYRGLSAHSVVVTEGGQVQLVDFRFARRNEGRAFTLCGNPEYLAPEIVEGRGHTESADLWALGVLIYCLMTGETPFASPGDDELRIYRRIVSRHLAFPGHFSPAARDLLERLLQHDPTARLGGQGPASLRHLKRHPWFAAMNWDALMEHRYMAPPGIRERIYNFEGVAYAHFDPRAYEGDMSWANNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9C0</accession>
    <name>A0ABQ5S9C0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_010395</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21224">
        <property type="entry name" value="CH_ASPM_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051185">
        <property type="entry name" value="ASPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706:SF1">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="716"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1763"/>
            <end position="1787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1824"/>
            <end position="1857"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1531"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1829"/>
            <end position="1845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI66525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1857" mass="209400" checksum="647063EFA53005F3" modified="2025-10-08" version="1">MMPMDGQRTNCYADQSLTRSHGDNPVTLRLIAYGGERPTVAFGIVTPGSIKDAVLQLLNETDLDRVLVLEKLPSGISLVEENGLDPSLGTFSVPRRSSRVLRVSWSPDGAAALNDSLRWRMAEGLRKVRLEVRLQGTVAPELPALARSNRAAGTITAKAADLRVLGKQTSPSSVTLKAPPKTLSLARPSLVAATSGTVPLYTPRDNLQRRAAPMDPLAVNAPAMVTSKPSSAPSLASPQPVLVLSAWQAANTPPRVSSTAEQSRVLDFRRVGCTENKRERALISWMNSEVATLGTEASDIGSRAVEGHVVRRLMGEVTGKAYVYFKRNESFAAMVSKIESKIAAKQLTIRDAEHTLSDVRMRQQALDVLTSYHPFWLAVGLQTILGRALVFSQGNMLSLMRPGAEVPAFIRAHILEHFLADQELASQIRQAALKHDYWETLGARVLGRVLLMVLLLDRMVQRHDLPAGTPSLFRQDAKIKSSEQVVQEFLQPRLAGAGDVRHSLRMMAYHTEYVQHARDEADFRVHKPMDLRDGTRLAKLLDNLRRQDAVKNGNNSKPMISQYSDSAVCHDISRFGCVPPAACSSRTQAPDAELLPTMAFPQNTGKPMDESQMRNNCLRFVRFLQQQGIGLQGLAAGASACLRDSGLDGIAKYIAEGLLRVDQKITLGVLWQLAAHYKLRHHVDVRSLEREVARLRRATGQIVAEDGATSTSYFNDPISQTLLQWMRAACAPYGVSVDDFSWSLSDGRVLCYMVHAYMPELLPLSSITSPELPSSVDEMVCMTGGAEYVKLETLKAKGWTAVYEMGGAIHDESLAAVCKRSVDANFAAIHTAGEALGVPAMLSAEDYLNDGPDEMAAILYVALLGEALLRLTSERRAAYVIMEFLRRRLSWRPDYMNASLGRFKAAMQRSEAASTIQSFWRMRCQRCRYLTVWRAAKMLQAFVRRWIERQRLQKQMHAIVLLQSGWRRHAVRSRLWRQGRSATMIQAAWRGFTVRQALARMLHAALVIQRHVRTYLASNRCSHRRLVVAQANAAVKIQTAWRGYAVHRRFQQLRAAAVAIQAFVRMRQAFNRYHFMWRAAVTIQASWRMRQEQQSFQRYRWAAICLQRSWRRHMEVQYFMAACSKVVSIQACVRGFLARREASVRLQGVVQIQAAWRAQQGRCLAKRRMLELEKSTQNRAAMVLQAAVRGHLVRRQMTQYVRAAVIIQACWRMASMHHRHKAMRVATVNFQTAVRSWLVQRRLREQHRAAATVQRFWRSYLELHAIRAACTAATVMQTAWRARRERSRFLTVCRAAVHVQRSWRLRFQARAAISLQAYWRMVVARRRFIQNRAIIIMLQTRYRCLMAMQQLEGTKTAAKVTQQSWRCASARRKVARRVEAYYMDRLRREEERLLEIARHYMLSTAAAKRIQAWWRGLLVRKAFLPIWKRHKELARQQRAAVAIQSAWRAYSTRTKYRRVLHAVKIIASVLVPIFRARRELALLRSAHHARVRAAITIQKGVRRWLARWHFLQAVAAVIKIQAAWRGHMVRARNARPRLQDLRRRLEQAADEARGAPQRQLGHRTREALDVLHLPNKNLSQVLAALEIMEVSTRYSRDCCKLVTQNGGVAALLRFVKSCNRSKPYGDMLNRTLAVLHNVCRYQELIPEVYYAEDCLTVLSELAQYYRDAEEVFIPTVILLQRLTVTCDLAANIPSAILRHWEGIHQVLFRKADIERKYLERLEGQKGSDVSARESARKLVIVQQQILALEVLIGRACQALKDGGAPASDLASDQDKDEPLRRVGPGRAGPAVAAVSAQVALPMPGFKNTLVKNVVQRLANAGTGVSTVRGSAQSGAGAGGSPVQSSKSSRPFSRRTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9E4</accession>
    <name>A0ABQ5S9E4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_009824</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF67">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="691"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="1612"/>
            <end position="1649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="400"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="453"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1020"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1144"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1200"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="401"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1063"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1151"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1219"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1285"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1325"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1376"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1404"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1649" mass="167983" checksum="372178F704C4E5F4" modified="2025-10-08" version="1">MSSREAQVADRDKYAERTTKMARVGRKYGVLFELAALAGSEPAAAPPEDWAGKLLRVMSSASWADPSFLEELSESGLVRAMCRALPVLKANAANRATARSVQDLADLEESMRKLRHELALAEKRLPSHGILGAGGSACYTTWGSIGSVCTLMLQALTYSAAWGQSAAASNSGVSPPPLDALRSSHVAASPASAVGVAAGEASTTPQTTGPLSQAGSTPLAALAAGVGGGALGVSTPRGTALIRPLGGGLSLSLSGSIGSGSGMPALATEGTLLKLLGNKHVVGHAADVMLVVPEQCKAALARILLSQINAEMQATRRGLAHILQILRGDPAATSPAPSVLPKAWFATATILAAYSEGLRPYLCAAAALPNEESASWDAGASFPDAPVAALEKMASLSTSDRGMDLIDDGEHAPKTSLPGADADSPPDRAATGSSGSPEAKLGNAINDAEMADAASAGAQTTEGDDSATEHNDLSLGSGSGVVEGAVGASAPETPPARKSASGASGMPATPSGEQASGRSAQADDPRGDLVQILQDVASALCEAVTGAHGVGRMAILLRGETAAKFPNAAAYMPAALSINELMGHLDGLACVATALGASDNIRTSVASLLTNQAMKELLGAGKVYVTTVIAAGALTRDLAIDAVIPQSLRQGYLAAVLNPLETGALLNERRAPQVLAYRNSITESSYYQVMDVEALPYGVNVRFEDEQEAEGMGVVREWLSQIAADIFSPERGLFVRGAADKRIIHPSSHSRLQEDYLGYMRFAGRIVGLAVRANVPLGVVLSTGLFNFLTGRRATLLDLLQIDLQVYTTCQNILSTSGADSLELFHVWHTSDEGGGEQELVPGGARLLVTDVNKNEYVQLLANHVVLTVVEEQCAAFLAGFQDTAHHLPDDARLRRAFLAPLLLEDLNRITAGESTLDPSDWVQHTDVAGFEGEEQGNLEMFWQLVSEYGPEDRQRLLQFWTAMTHLPSGGFKALNQRLQIMKLGPSSVRHADGEPVVGDRATAVAVAGAAAAAAGITPAIDGRRPSADGVGLHSHRLDGDSLDVEEQEHSDGMEGLEDAIPNTAQQQRQRGGDSSTHGRTETSAEGFLGSQAAGDAAPVVATDPPRSEGAAIEAAMAAVEGAAGTVAAGIGAVSAQVPPPAGIHGTDGTDAFDEPDIPYGDDGEDMGEGEDGMDYGEEAEDEEFQRQLEMALALSLQDMSAAPAQGSGSENGGGGAGAEAPGEPAAGVDSAGRAPDATAEDTTQAGETPSDAAVNPGEAAAAAATEIGGISSRPEDAASGPLGDSDVTAVATATADAARGTGSDPDAVAALQTALGSGAGEPNAVASSPPGGSHSGAVPGGDSDVGGSYSNSTMPGTRGCTPTAGNSPDARAIRAEVASVAAAEEAAEEPDELGPSRISSDRASAGGAQGLDDGAAASLDADSAADAAKAAQQSGAEPMDMDSGPGGTSFLHGVEDGTAAGDAPGRPEEVNLKNTDMETDVPASGLYQGREDVEEGEGPRLPQEPLMGQNQRISEDEPDRQRAAGVLAQLGLGSGQEEWDAAEGPPLLQANAGQVEGLQEQQDTLPQATTAVPVMDAVDGAEGAADDGGGSGMATGGATSQGASARVLPLLPQARTCFLQLNLPVYADLDDMRRAFAIALDNMSFGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9H0</accession>
    <name>A0ABQ5S9H0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="182"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="237" mass="25245" checksum="149FFCEDC43C7633" modified="2025-10-08" version="1" fragment="single">SLLARSPTGTGALATANTAGGTAAAHTLDPLNEMREMEDSEMHFAEELPTDLHFLSDFTPEDAQQQLHSEGAQLRATTPTCGQPAPSLVPLPSIGAPMDHFMPPSPLHDGEEVALPDATAGAARLSTGRDASTAATPTDMFGRKRRRYSDEDNDSASEANSSLLAITDQQREQLAVPDGGEDASNNNNAENDYSAACAQDDANATGEAGQRKRQQREPERPRRRRRAGGRVTLDNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA93</accession>
    <name>A0ABQ5SA93_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyl-accepting transducer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000029">
        <property type="protein sequence ID" value="GLI66262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="45838" checksum="4B798DE51EED0AD5" modified="2025-10-08" version="1" fragment="single">LVDCAQESADEHGEVQQREAAAAELRATVVELEKRHEAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRRETAEAGAAELRAAVMEGEKRREAAEATIVQLRAMVVKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAG1</accession>
    <name>A0ABQ5SAG1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010944</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000049">
        <property type="protein sequence ID" value="GLI66912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="143" mass="14876" checksum="C5D75FBB932FD943" modified="2025-10-08" version="1" fragment="single">IVFVPTTVSASAVSTPQTRRSGTSAQSLRSIANQLHARTSKFLKFIEGIQALVSNHLTPGAVDIPGMAPAAAATTCAAVAGTSARPPGPPHTGHEDEVIKIAIAVPKLNIPMDVKLSDLGRVAAPLTRAFVRNVRNYFATARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD29</accession>
    <name>A0ABQ5SD29_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="192"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="343" mass="38378" checksum="87D43B96F6948C67" modified="2025-10-08" version="1">MGMATTFCLRSSHLKNNFQPRRQRAEVVQDLPIPVIWYLLPESSHQGSCTENSLFTAALSTANFPLRHRRSLMHLSQDSNFAIASLVCSVTSGTNSHARITKVSATTSVFSSQTALQRSTLPWASRFTLKTHFSPIFHALMGTSSSSSSSSLFAGRGRECLTVVRLGTSLIFARRRAVRRWAPARYPIPQDTTSSVFFHGELEEEVYTKQPPGYQLGGPNVACKLRLALYGLHQAPCAWYVWLRKELESMGFKPSLADPGLFVKTENSESVYILVYVDKLLVAYKSLAVIGQAKAQLLRAFVRHDLGETRFYLGFKIELGRAARTITITQKRYVSNILEKFGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDE4</accession>
    <name>A0ABQ5SDE4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="255" mass="25752" checksum="89C89CF798353DD2" modified="2025-10-08" version="1" fragment="single">GCSSSASLNARYLPAAAAGTMAGGPVAAAAVMMAGAPVAAAEWNADFRILSSESDGGGAGNAANAPTLAATRDCSPCGSRFPNQEVVDGGKAVINGADAIGAFGGDVDGNGGVAAVLPVAAVPHAVWHRVHLRAVQVPVEGDVAAKAATSAPGGIATGGMPAPVSLLPPPPPPPQQQQQTERQQSMLLVITQFDVTAEVTAQRETAGLLQEEHKVLESIFPRHCIEYLTLRGGAGRRRGPARTGPWNRGPRGHGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDG1</accession>
    <name>A0ABQ5SDG1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="180" mass="18616" checksum="51B2C4345EA75C7B" modified="2025-10-08" version="1" fragment="single">MIVLTARSERSRIASGIIPDAPCVNEKAAEEDQQQQGTVVSNPTNDGDDEADFNTTSRPKRPHIPYSQREIYEKKALAPPRRPTAYPSSSRRTTGSSDGGSTPVHQHAPISSPAYSHTRAISSPSTSTHSASYGGAGAGRRPSTTSIHGSSAGGGAGTGRRSSASRRTSATPTRGSSAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDT2</accession>
    <name>A0ABQ5SDT2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_012413</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF261">
        <property type="entry name" value="ZINC FINGER CONTAINING PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="228"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI68082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="546" mass="58753" checksum="16A8CBA2D3A1A841" modified="2025-10-08" version="1">MHIKADCATSDLVILEQIEAANRLRSMLVAQVENDAIILDRTELERDAWAAHAKSLAEKLVQARKRIARLERALAEASGQPLLAASSLCNTDDREADADEDARQVTELLYLQISQASPDLQAATGPSAQSAPASQAQRRAESAALYPASLISATAEMQAAKTASQDAVESSASSGAAAGQGDPAWATGGAGPGPGSVTFNPTPNQQRVLEALVQRGRAMGWLIQPGELAMGSVLGEGEFGITYLGMWRHAAVAVKVMRLRHSAELTTFLREVESMSYIRHPNVVPFLGACLSGPDKLMLVSEYMTGGTLCDWLHPGGLPAPGTQSGGLTRPVKPLLERLKMAHQVAVGMCALEACRPPVLHRDLKPANVYLDQRGLPRVGDFGLSRRLLEQARASLTGETGTYLYMAPEVMRHEIYDTKADVWSWGVLLSECITCAMPYSNMYMTPVQVALAVSAEELAPTAPPNVHEEVQVVLRLACQFDPEMRPDFASLADMLGGAIKHIEAQEVAKPTLMASIQSGLVGLWSGGWGKNSSFSRAQQQQRQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE01</accession>
    <name>A0ABQ5SE01_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="253" mass="27127" checksum="039B650CA8EE37A3" modified="2025-10-08" version="1">MFGGFATCYFFPEWLRCLWAPDTKFRAQENSGQIIIDIISPRAFSSSCMDAPDRRLWRPCPSPATLTTDGDCLLLDKEEDRLQSDWSYANNLTSDRSSHGPDVFDSASACCSSNGSSWSISPRSFNSPRGGSESGNDDGEDALVYAADSWGPVGDLMASDEKEALTSSYNRLLVASDVSSASAPCRITPRTSQHYITQGYGLNDFNTGAAVCEATLAGSLGANALQHAVEVPIPDIDGLLASVIATALWQDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE07</accession>
    <name>A0ABQ5SE07_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012501</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="21"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="325" mass="35268" checksum="CE29CF9CF4B93802" modified="2025-10-08" version="1" fragment="single">SSIPFSQGALLCAPDRLAVTMASFKDQGNDEFKKENYLKAAALYTQAIKEDPQNAVLFSNRSAALLKLNKVTKAMEDAEKCISLKPDWEKGYFRKGAVLEAQDKMAEALEVYQAAARINGDGVANKELSNKIRSLSKLLKAKSFTHKEDTLESLLLANAAAAIAGADAAQQQRLSEFFREMAAMTLENVAESGTTFPPSLHFLPGAAAEPHEEREAHIQAANAFGSPELLGDFVMGMRETSKRLAASVALAVVPKAAVSFPQLWMRRAWPSACGSKQQAGVFVQLEARGAGSRHIWFVPVRADKSTSPPVALNFDDFGLLPQLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE82</accession>
    <name>A0ABQ5SE82_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">VaNZ11_011891</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056759">
        <property type="entry name" value="DYH2-5-8_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF32">
        <property type="entry name" value="DYNEINS HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25007">
        <property type="entry name" value="DYH2-5-8_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0243">Dynein</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="1842"/>
            <end position="1978"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2122"/>
            <end position="2334"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2494"/>
            <end position="2662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2357"/>
            <end position="2393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3310"/>
            <end position="3379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="2375"/>
            <end position="2388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GLI67642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="4522" mass="511816" checksum="14114A085C09E613" modified="2025-10-08" version="1">[encoded data, 4522 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEJ3</accession>
    <name>A0ABQ5SEJ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">10 kDa chaperonin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012738</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF63">
        <property type="entry name" value="20 KDA CHAPERONIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="14073" checksum="78FC81085E069CB9" modified="2025-10-08" version="1">MATFKISSGCCARFAMRPSVGRRTAVRVAASITAEKASLDITKMTPIHDRVLIRPIEEEARTAGGILLPKAPPKANSDAHIGEVLAVGSDVSLGLAKGDVVVFQKYAMAEVEVKEGQILFVAEKSIMGKLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF39</accession>
    <name>A0ABQ5SF39_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012991</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="10926" checksum="4F68986EBAFF9F1C" modified="2025-10-08" version="1" fragment="single">ATSMVNITTSRLFEDSGNRTLDLERIFYKLLKRPGRDPGTFVHIVNSAFLQNSCSDVIGVAGGAIFSKNAIFIQLSSFISNICPTGGAIALSLLNPSDYVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF57</accession>
    <name>A0ABQ5SF57_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_012665</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="691"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="752"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="787"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="825"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="850"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="932"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="960"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="94"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="410"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="704"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1057"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="638"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI68304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1320" mass="146010" checksum="16146E42F43B9081" modified="2025-10-08" version="1">MGGTTTPARGDDPTAGFFNKFTFGWMFKHVSDARKNGDLDPDSMGMPHENHAHEAYDQFASNWAAEMKLKEAGGTEKPSLVRALRKSFGWYYMLGGVCKCGWSTFVITGAFYFVRSLLAHVNGITDGRLYSTTVAGWCLMAGFTLDAWLLGLSLQRMGYICMSVGIRARAALVQAVTHKSFRLNSVRADQSAAIVNFVSSDIQKIYDGALEFHYLWTAPFEAAAILALLGYLTNDSMLPGLGVILLVLPLQYFFGYKIIQIKLQNAKHVALRSSILQEVLPAIKLVKYYAWEQFFENEISKIRREELRLSFWNALMKVINVACVFCVPPMTAFVIFTTYEFQKARLVSSVAFTTLSLFNILRFPLVVLPKALRAVSEAHASLQRLEAYLLEDVQQGTAAVKSTKSSVPGVHIDNAVYHHPSNPNWHLHIPRFDVRPGQVVAVVGRIGAGKTSLIQAILGNMVKEHGSQQVGGRISYVPQNPWLQNLSIRDNVTFGEAWDENKYEAVIDACALTMDLQILPQGDQSKAGIRGVNFSGGQRQRVNLARCAYADADLVLLDNALSAVDHHTAHHIFDTCIKGLFAEKAVVLITHQIEFMPRCDAVAIMDEGRCLYFGKWNEESQQLLGKLLPITHLLHAAGSQEAPPTAPKKKDDKATPQKSQSLQLTLAPTSIGKPTQKETRNIPKLTAFKSALIYTYYGNLGLVAICFLFFLAAQTCRQMSDFWVRWWVRDEYKHFPKPKGVQDESATKFYCLIYLLLIGLFYLTMLCRGATFLWWVLRSSENIRRRALRNVLNAPMGFFLVTPVGDLLLNFTKDQDIMDENLPDAIHFMGIYGLILLATTITVSVTINFFGAFTGFLIIMTLIMLSIYLPAATALKKARAVSGGVLVGLVAEVLEGLNVVQAFSKQEYFIEEAARRTDVTNAAVFNAEALNLWLAFWCDLIGAALVGVVSAFAVGMKDTLGAATVGLAFSNIIQMLVFYTWVVRFIAESISLFNSVEGMAWLADYVPHDGVFYDQKQLDGVAKSITLPDGQIVPAITKVQVVVDDAALARWPATGNIRFEDVWMQYRLDAAWALKGVTFKINDGEKVGAVGRTGSGKSTTLLALYRMFELGKGRILIDGVDIATLSLKRLRTGLSIIPQEPVMFTGTVRSNLDPFGEFKDDSVLWEVLQKVGLEAQAQHAGGLDGRVDGTGGKAWSLGQMQLVCLARAALRAVPILCLDEATAAMDPHTEQVVQETIRKVFDDRTTITIAHRLDTIIESDKVLVMEAGELKEFAPPAQLLANRETMFSKLVDKTGVAAAAALRKMADEHFSKSQARSAHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFA0</accession>
    <name>A0ABQ5SFA0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31033:SF18">
        <property type="entry name" value="OS06G0115800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31033">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="480" mass="51339" checksum="85561724D7D46752" modified="2025-10-08" version="1">MAHPCFSGNDYLGAAMKKCPFLHNIAQTHGTEYASSLACKTSCPDGAVSAHRPVLADDYDEDDFSSTFALFHGRGGVLPLRHKSAEEPKVLYPLEHPYATAERERPRCPFPSGAPKSETSSFAIPLFAAAPLASMSMSWGSNWFNFHHLFQKHINEHRAQQRKPPQQPQQANGNHPGGVTGPGLGGPGSKSAHGAGTSGGGKCPLRRALGPLTGVVFNKHGQLSCPEPIVKMRAALAATRPVRELRPQALPIKLLAVAMTTAALNVPCGMWREHTEKFSSQWFVAVHATIPFIAMLRKAVIMPKYAILFTICAAIAGQGMGTKLERRRLMSRAKSELGGLAKASVAATACRPSAAAAERQPAPLPVASLLMSAQPLDRPAMSTPAPSHGSHLWEATQGDNQDSVVAATGRLMFPGIIDSDQQQEEQTSRQLYFRVSKMRATGGRPAGGRRGSGSMPALSISRDSLASILPTMAMFSEVKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFB1</accession>
    <name>A0ABQ5SFB1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="405" mass="43366" checksum="4EA2BF4B906AC17B" modified="2025-10-08" version="1">MRVAFSVRRLSSASRSSSFPPRGSAAESSLDAADSGSAGSVFIGAQLQQERNFSAPMFRSRGGFGSSWSHQHERQTTANLTSSTSSLAATAAWQASVVNAESCQVEDWLAALHQVDAEARGLCTDLLSRHPTLLWKDAQRTTGVVQALVDATRQRQLKRPYFMQDTHILALVDRYTPESLRARLAELHRLVWQKLDDSNVTSLFTSRTSLDMLDLDAKALALKAQALRALIPEFLPSAVLRGCPELLDVQVDDLPSYIHRAGNVVVVGAHVTTELVGVNNVVIVRSKVPSDQVIAPTAGAPASGPTTATTASTVATAGRLLRTARCGAQQLLHEQARAAHGVAAPREVVRVFCNSRQLVFAGGEQVEVRVEDPSKAQEDLSAACRMAVLQRLSVGGQATAGARCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFM8</accession>
    <name>A0ABQ5SFM8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013233</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15615">
        <property type="entry name" value="PHD_ARID4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042293">
        <property type="entry name" value="ARID4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46694">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46694:SF1">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ARID" evidence="7">
        <location>
            <begin position="624"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="728"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="449"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="538"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="582"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="538"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="588"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="799" mass="82974" checksum="0126740B7D21E42D" modified="2025-10-08" version="1">MASVPSWRIVACYGAPVGEGTGLGVFSVLLDLATPETQPSNVQVSALFNPSLEEFREAVLASRPNLIYCCGSPSHHDNKPTGTVGAFHFRDGVESGESGLLTALEEAQAEVVYVDAAVSPHFGPSLHLRGTPHVVVWPADSPIPAAVASYFTAAFLAFLINSGASPPEAFATASHGAQAHCCCSSSSSICSSPSIVSATNGTGAGNSSPPLPAFVSAERPGLPDSSSIPALVVPGLQLDPLRLLSYPGWSDVRLLAPRAELRLLLVGPAALIDSSRLSFLGEALRALLVMEVRGLKLVSRAPLEKLPRTLPGGCQAVRCQYLTCSGAKGSVVLGAPPSVLEHDGLVQHALRMTLVTDSVSLQFRLPPPGLSLPAPRSSKAVAGGLPVVDTVAVTSVWAVEVLRSVCREHRYHSLAALGVAAVGNVADGAYTAADVRRFDVLVAGSWASPPPPRPPIAMTASGPAPGPLSVATGEALPSPAAPPPQGGSESLDLTAAAASGSAYFGNDALMGYALNGHLQLLAGGGAAAATTAGDGTATATAAASPDSPDGGAGGADREELMGPAASDAAGLTLDDLTDGTGFVSATDPGVRGPGRPAGPLSDKRPNLTGDLPGYTCRRPPLSSCTEAQFYDDLVDFLTLLRGKPIDRARFPEAVLNGVSLDLFSLYREVVTRGGFRVGNGINWKGQVFPRMRNWTETNKQTGVGNALKRHYQNYLWEYEVAHPEDVTLDRCVLCNGGDTVSSDWVCCDACENWAHFSCDKRPGIGAFKDYAQGQGRVYVCPSCSREHEAGEAVKRQRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGV7</accession>
    <name>A0ABQ5SGV7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013761</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="340" mass="35644" checksum="EECD7837ED670F45" modified="2025-10-08" version="1" fragment="single">MPALVLLGRQLLIASDDVPLFALPAALFHSAWTIVLIVTLALYQRHPLCRDEGDYLPFLAGGTGAFTISSALEWSLFKVGLTGTPLETHARRAVPLLLYLHTFASVAELGFAAYGAHLLHVDTRLCKAWSPRPLAAALVGTTWAVIALNGLLTALALNPWHGLGLVEAWEARLLWLARLLCCRSRLLTQARLPDREPALRRIAQLTSHLLQHVDLTLSDVAAAMILVAAAQRERRRRALRDAMVAAAAAHSVLLGSSGSVTAWQGSGSTSGLKPAHGSHVDTVSASPRPSKVVGSPGSMDGGTSVCSLGKEGLPAPAAAGVVLLHGGEAGPVQESTPSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHW6</accession>
    <name>A0ABQ5SHW6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase C14 caspase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C14B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12660">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050452">
        <property type="entry name" value="Metacaspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104:SF30">
        <property type="entry name" value="METACASPASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104">
        <property type="entry name" value="METACASPASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C14 caspase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="406" mass="42552" checksum="2C714A259E68F12C" modified="2025-10-08" version="1">MPSKRAVLIGCNYPGTNAALRGCINDVWGMKAILEEHYGFQTSDIVILIDTDSGYLKPTGKNIKEKISEMVVGSQDGDICFLHFSGHGTQIPSRNGDEKDGKDEAICPTDMNVICDDDLRVLLKPLEAKPGVKFTFIADCCHSGTLLDHETVQISGPKDGAPPPPQFDMGALSSIFGALGQPGGRDFKNRALPFNDLCGMLSELLGGAQVDARNVRSNLGSLFGADSSVKVQQFMQVFQAFTAGNKEGGGGLSGMGLLQLLCSCLAPSADQGPSGQPADANSGPGANYAAGNLAEPNLDINLPPAGAKPPGDQQLAADTGILITGCQAHETSADACPSGNPDKAHGALSNAIQTVVRTHHQQNPGQPLTYRNLVIGVREVLSKTGFAQNPCLECSDTNADSPFILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI53</accession>
    <name>A0ABQ5SI53_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Epsilon tubulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013433</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02190">
        <property type="entry name" value="epsilon_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004057">
        <property type="entry name" value="Epsilon_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01519">
        <property type="entry name" value="EPSLNTUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="7">
        <location>
            <begin position="57"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="8">
        <location>
            <begin position="279"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="53214" checksum="EB6EA1D1388F0971" modified="2025-10-08" version="1">MPRELVTIQVGQCGNQVGCRFWELALREHAAYNTKGVYDEALSSFFRNVDTRVEPPRNLPVGDGRGPIRTLKARSVIVDMECGVINEMLKGPLGEVLDTRQLVSDVSGAGNNWAHGHHVYGPQYHDAILDKIRLTTEDCDSLQSFMLLHSLGGGTGSGVGTYILRMLADEFPGVFRFTGSVFPSEEDDVVTSPYNALLALGQLVEHADCVLPIENQALIDITNRADAAREKAAGGGGAPGGTPAAPTTAASSKPFDSMNSVAASLLLHMTASVRFEGPLNVDLNDITMNLVPYPRMHFLLSSMSPLLPPPRERDVGRQALALQPRTLDQVFGEVFSRDHQLIRADPRNATYLACGLIARGPTATISDINRNVARLRPQLRMVHWNSEGFKLGICSTPPVGCPFGLLCLANNTAIASTFTTMKERFDKLYRRRFYTHHYEQYMDCSGFESAIELVADLTRQYQELDGATRAPAITRLRPRGLSFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIN8</accession>
    <name>A0ABQ5SIN8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="530"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="615"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="886" mass="93955" checksum="7C84897F549DC96F" modified="2025-10-08" version="1">MPLSSGAARRMKALSPIPTPRADDAMAEVRSTEQPTVFKLQLRPLEDPESFKPSVQLVSREFATRYLESVGVRPSKATMDLVNRNVPLTETHIKTTLRAKGHVEDEIHISVPPVTHRYRVPAVINPAAVLQLGIGNSVLGSGTEKLGDTEGGPGSSDSLEELFNGRPGDSTAASRAPGGTDFLDMATKYLHPFNLDLTLARKSLTTLQAASRHKSPQGGAGDRDSPLVGGAGGGSGIGGIGGSSFGTSTSGRGGATGLFDGRGGALSSRSSFAASSRRSGSWPRSSSGRLMDDSGVGGGALDATRLEVLEELTQPLLVTPEVVLTLQGQLEAALADWHRHQAPEALKGRLTTEEFNRTQEELSSEGMAKLLITLVNFLHEELVRGHPSYPAQQSNARENSRTSVLAGERSSQPRSIGGSGDGGSGGGGLTGRRSHAGSVNFRGSVQLPASISIQQMTNLQKYDLGYQAYVRQKLEQRQAQGTAASNTGCRGDGVDNRSQLNTPPDTLGSSRATGLGSAGDRPIVTSAVTQAQPQQSRPQSQPQSQPQSQPQTQQLHRHHPMTADTQRVSFAVTPLYSSLPGSPQPESRPASPGPRGGSNGTSVFGPLNLPGNPRSRPNSSHQTTPVTASRNQRSASPANAANAGAGGGETTDPGLASPPILGLPPHVGRALRSSPSFALASAANTARSIHASLDAEDMAALQRERLFALHIDFASRFKALRQKRSGMFFTLPILLLSMRLCVNLLFSTLYPLWTRQPDGAEVLEQMDAALCALFDPHGYLQRALSLLQSTPSAIEAVTRHPQRRGNERRHFNDTSPLMQATLQGPKSAGARKLMKAAANQPQGLGLMRTQLTTEQRAALFQMGLQWMQQPDPVLSQIGEPHAVGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ26</accession>
    <name>A0ABQ5SJ26_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Helicase-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.530">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005114">
        <property type="entry name" value="Helicase_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33418">
        <property type="entry name" value="HELICASE-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33418:SF1">
        <property type="entry name" value="HELICASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03457">
        <property type="entry name" value="HA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase-associated" evidence="2">
        <location>
            <begin position="281"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase-associated" evidence="2">
        <location>
            <begin position="348"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="419" mass="45134" checksum="C03DBC7EB129655C" modified="2025-10-08" version="1">MLLSSRRLACLGHGNSKPRMRHCATVDRSAVSGNVRQLLDSFWEARGVLDPVHRKRLVDAAEALDAEVVHPLNSAPLSADSPLPGLPASWQLEESIPEVLAASRRLLALQQMLGGGLAPDSSSSMSRSIGAIDVVWMVVREPGLLSADLTQLTGRLLEMKLATYGMDVDVLKVIEAQPALLLSDRWRLDPESLQAAARHATASIAPVPSSPPLAADRDPFDGSGSDNTASGSGSGFVEGSRSTAGPDGTAVNSPGSGAVTSSGSVQQLVQAWQYGIASDGDQEWAVRLAQLGDYRARYGDTCVGFRDGDDAELSRWTTKQRADQRRGELAPERLAQLTSLGFHFDADEAEWCRWFSELLEFKGLTGHASPMPLVTGCDMYLINWCSVQRIARRSRVLSESRIQRLDAAGFDWTGADPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJU5</accession>
    <name>A0ABQ5SJU5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015111</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="632"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="909" mass="98508" checksum="D6B0F4B6A0ED6259" modified="2025-10-08" version="1">MYQLSGLPSRLMCFALGNEERGYSLNCFFTWTKASNSIRLSGTRLCPRPCYVAVPVLTKTFFFIILAVASVLEARLDTRRGITEEQNVSPSLTLAMERRGRLPLSSSSLTVSDGYQFVAALLNPSIDLIKFAAPVVDVPVDAWIIPGIELPIIITRNITTEGNEDGWPLLRLHNVREKMRLEGNVTWTIRFVFLHGAFGDNVYRSQGLTILSPTAPNQTSEMLLGPAAVSFRLCFQAPKYQALTLVDRPTRYPGNQTVRLDVPMTGCLDGPSVPPLLRCWASKAIMVDIAGQGVMINPASDLPVSTNIWFWMRNVTGFCEHAVDANCLAKPISEPLICYKEAEQRHQNDTDFLDDYRPQWGQSGGTQSASVPQQSAVIPTSGGGRHRKAVVGVVAGAVVASVLVAAVMAAVVTVLWRRRKRRHTDSHSVIISESTLKSTFGWRMSSPAFNNAPCPDMCPSPGAGGAGLNSIFSMGLPQLFNTDRDLSAHQSQDKSILQTVLVTAEGDPVTPNTEPKPDLDLHVRLRDANSAPTSRTEIKGSAANNNGHKPDFGTVSAGSDSADGSGQKEFPAASGTNGRQQVENDAEGDAGPQTAVTNYNNSFLAAVSCHTISAGLEVASGDGGSGGSGVVELSQVVRGKGAFGRVMEGTFNGERVAVKLLACDWAWGEPTETFTRCFAQELEVLSRCQHPNVVRLLAACVDAPRLCLVLELMETSLDRVIYGDPTAPLMPLPKVLHIAISVAAALAYLHPTIIHRDLKPANVLINDPQSDRPVVKLTDFGLARLRVTVMPTEHPDAGTPAYMAPECFDVQDNLVTHKADIFSLGVLLWEMLMCKRPWEGLANPLAVALMVAYSDARLSLSDLGEDRCPPKLARLIEACWERDPARRPAAAEVAKHLALMQQQIQLQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJV8</accession>
    <name>A0ABQ5SJV8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuole membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="49117" checksum="A43F3CCC2CF1DC4F" modified="2025-10-08" version="1">MADSEVEDEAITQLRLRQAKALRNVTLFKSPVQTIYYCGCYAANGFYSGAKWVASHPVTLVILPALVAYAGTKWAGYKQNLLTEIEENVKYVVWWVGLGVLSSIGLGTGMHTGLLFLFPHILKVCLAAETCGHFRFDTRYDMWYSSESFKCGEGPGEVVSFADLFKKVILTAVLWGIGTAIGEIPPYWLSYSAAVAGQKNVALVELEEALRPSHQRNLFQCMVARMEQWMVSFIRSHGFFGILLLASWPNAAFDLCGLVCGNFRFPFWKFFGATLIGKGFIKVTGQVVFFVAVFRRDSREAILAWLERLLPARLPGLGSGAQSPARELHLFINRSIAKYQAKVLAKSTAHLAERRWWWRRGLDVLRSRQELQRLMMALIPDTIAEVWSGFMALLMAAFALSCVNAFAQAYRMQLDDAEVIAARQRLAAAREQERKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK15</accession>
    <name>A0ABQ5SK15_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05382">
        <property type="entry name" value="CAP_GAPR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034113">
        <property type="entry name" value="SCP_GAPR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="432"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="654"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="735" mass="79025" checksum="BAB94C0B8855F956" modified="2025-10-08" version="1">MRYGTEKLRKAAVIIHMDGERRRPAGGSLDCIEAIIRISSGGNLDHQLQQHGQQQQHTATPPTNGRTTDERELAPPACSMSLLATTFITHLGIVLSDMAPRVALLQAIIVVVLSYSFHLSSGEKTAPQQLHLQGHEEQPRRQSTLTEVHVVGDSSISTDEVVQSKSWLLAVTGRWSSAAAEVISSWSRNSKMMRRLQQAAAVLPLARPPSRRQQPPRPLAPSTPQIYKPPPSQANKRSPPPKRPPSPSSPARFPPSLRSPPQPKPSPPPLRPQRPATRPPRVPLTSISPPSARPLPSPPRPLTRASSPSPRPPSLARLSPPPQPPPQPPPQPPPQLPPQPPLKRQQLPPPPPPQISKPLPSPLPFPPPPPQKLRPTIGMIVEAASPNPPSPSSRPPTRSRPPPPEDYNDPLVDESPPPPPPPAVIEEDGGCPDAKEALDWTNVFRNRHSTAGLTWNSALADSAEAYAKVLVSKCKLVHSMGRGYGENLMLWRRYPPPRSWSTGCASAVKVWYDEVQFYSWDVVRPYSENDFGMVGHFTQLVWRSTSSFGCGMAVGNVSLPLYVNGTAAPWGCQIVVCQYQAPGNIATDGAFLRNVLQPRRGEPIPAPDDISAPLNPSNSKSTRSKAEEETIIAEAGPVYLGPPSSEEGEDEDPGSSTDSDSSASNSESDSGSSSQGQTTPPAVPSPSSPPQQGTYDGEFNDESGSGDSAGQPMWQPPPAVAIYGGSGSYNSHHPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKP3</accession>
    <name>A0ABQ5SKP3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peroxisomal membrane MPV17/PMP22-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015486</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF116">
        <property type="entry name" value="MPV17-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="21185" checksum="B8636D06031BFF28" modified="2025-10-08" version="1">MAPLPAPFLRRMAPVARAGLTSCIIMSAGDIICQSLQRRNKSGALNWASHDWKRTARFGLIGATLHGPFFLWGFQMIDGRFGPATNLLTAARKTAFGQVTIFPAYVAAFFTYITLLETRGDVAAVGTKLRNSFAQTYLAGSLFWPAANMINFMYCPPSARILYVNCAGLIWNAILSAFNTQGAVAVGRQAVTASPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKW5</accession>
    <name>A0ABQ5SKW5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutathione-disulfide reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_015567</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004776">
        <property type="entry name" value="PRK06116.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF9">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="10">
        <location>
            <begin position="46"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="9">
        <location>
            <begin position="394"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI70632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="55103" checksum="12E643B215DF7F96" modified="2025-10-08" version="1">MNIAKLTSGVATRRCLRVAAPGCVGRDLGTRATSTASAPAGDFDFDLFTIGAGSGGVRGSRFAASYGAKVAVCELPFDYVSSEARGGVGGTCVLRGCVPKKLMVYASEYADDFRHAGGFGWSVPSDGLRHSWTSFIEAKRKELQRLNGAYKNTLRNAKVTLIEGRGRVVDPHTVEVDGKHYRAKNILIAVGGRPSKLDIPGANLCITSDEALELPECPRRVAVLGGGYIAVEFSGIFARMGAEVHTVFRQPLPLRGFDEEVRKFAAEQYTAAGLTLHSEVTPQSVSQQIDGRLSLVVAHKDGSSEVLADLDQVLMATGRVPNTHGLGLAEAGVALGPKGQVVVDEFCRSNVPSIWAVGDVIDRIQLTPVALMEGMAVAKTVALDTPTKPDYWAVPSAVFSNPEIATVGYTEEAAAKTFSDLDIYTTSFRPMRNTVSGSPLRTFMKIVVDATSQRVVGMHMVGAEAAEIMQGFAVAVKMGVTKSQLDGVVGIHPSAAEEFVTMRSVSRQVRPVPATVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLJ0</accession>
    <name>A0ABQ5SLJ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="4">5.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015815</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018427">
        <property type="entry name" value="Isopntndiph_ism"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="87"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="283" mass="31585" checksum="5AD6B9E58B16B7FC" modified="2025-10-08" version="1">MLSATIVSNWPIVARYSNFLGQPLEVARSSAARGQMAGASTWAGTGLSQQDFMQRDECLIVDESDVIVATANKYDCHRFTFAQPRGLLHRAFSVFLFSPDGKLLLQQRAASKVTFPSVWTNTCCSHPLAGQDPNEVDSADRIADGSVPGVKAAAVRKLAHELGIPPEQVPPASFTFLTRLHYCAGDVHTYGPAAEWGEHEIDYVLFVRPLQPVTLAPNPDEVDDVRYVDRKELAAMMNPGSGLRWSPWFRILADRFLDDWWQHLDEVLEGSKHQDYETIHHIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMJ8</accession>
    <name>A0ABQ5SMJ8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015561</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="117" mass="13645" checksum="222A7812C8283559" modified="2025-10-08" version="1">MKDPGMQSSPMLFGRKEYAVVKQAVLTQLKKKTCSKAIDSDEDRRDQVSEMAEERAYGIISEFLDPMDRPQYEECESAWDLWEAIRETYEQVTKASVMEIFHKLKNFKMLPNESIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMT8</accession>
    <name>A0ABQ5SMT8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small Arf-related GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_016096</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22539" checksum="C3382DCE87D28ECF" modified="2025-10-08" version="1">MFSLLYGFYEYIFRKDEFHVLILGVDKAGKTNVLERLKTIFTQSIGLDPGKILPTVGLNVGRIEAHKNNIVFWDLGGQSGLRSIWDKYYSEAHAIVYVVDAANRSRFEESKVALDRMLEHRELTGAPLLIMANKQDLDGAATSQEVGQAFSIERVEGRQFKVLPVSAYTGQGLKEGVEWLVETVRRSHRAIRLRVRSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMU5</accession>
    <name>A0ABQ5SMU5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="779"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="849"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="906"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1014"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1430"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1538"/>
            <end position="1719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1789"/>
            <end position="1816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2117"/>
            <end position="2140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2241"/>
            <end position="2308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2408"/>
            <end position="2453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2662"/>
            <end position="2690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2736"/>
            <end position="2810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="572"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="781"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1033"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1545"/>
            <end position="1563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1611"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1657"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1795"/>
            <end position="1807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2241"/>
            <end position="2260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2265"/>
            <end position="2280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2297"/>
            <end position="2307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2420"/>
            <end position="2448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2662"/>
            <end position="2677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2741"/>
            <end position="2750"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2962" mass="307915" checksum="F156AB30CB4F2C39" modified="2025-10-08" version="1" fragment="single">ALLQLRFPKQANLLEMLLHQTVGKDTVDRATSVPIAFSTSTVLSAPAGMVNAPVPPGALEAVLERRLHAFSVLPRPVPLGSSPAAPSRTFLPSPIAAAFQELRLCVPHRIGSHHVDLTVALPQHAARLATAEMTGGRETKPGMGMITGMDSVLFSDLVELDERGHVHGQSAEITPASEGQQERRHAGRGMRAMRDMLQPPDSQLWALILSELHALDVINKPVDALIQDSNSCATSSGSMGSQRQLCSIDLSALHPGLTASVVVLHLGARLPSELQAASSAGNAPGTHPYSGGSGSGMYCNYSGVARSPVAASDAVSHEPALVVRRHCVVAIVVVYVLMDQDKASWELWSRDGAGGGVAAIADTVATTRVREASFRGLSYSVAIGPLAAPPVVRAVLTAAAWKYEQAFPSTAAEVWRELRMVASEQHRAASNLEVDEQPAEGLAMSGPHLQRSTSIKAAELNEPTLPPISAMSGSPERICTLSSQMMLENLEAKSSAPEASLVPTQMGDIKVTDRTANCGNAASSTEITGQGSEKAQKFAVNLTAPIQGSAAASRGPSVFATRASPPDWMLGSLSSSSSSTTSPISRLNSGSSIRPANLSLPGLEEMPVASPAGAVTSGVSEPSGASLGPAPEWSNPDRQPPRDSFVPSSFVGVANAGERRAAAGSHDDDDAVLVVSPDDGASAAAVASADGPFARALEAAQAAAAENVEESVSRISRFGLGLGNGMAGDIKALALAPVPPLALAADTLNLSPTPVMAVALGEGRELAASGIGGVIGGQEIPVSTSTRPTGGSSPGLRASITRSGADAVAAAVVAVSEAAGGLGVPLGVAGGSGAAAAVAVAGSSPLAVLGSRSQHGSESSNRDGSARAGGMVAAAPGAISASSTFVVTSKSTGVSVETATAAIMDGQSQGSSGNHGEISGKSVCSGNSRHPDMGSSAGPWPQRRASVPLLRLSETALSGAIDECPTPSLIHFSSLSSLSRFKQSGNSVLGTSETDAEEQNTDVLALKSQLRQGFPAADASTATRITEPRQGPQFRSTQPGSPGASSPTANRQQATGEQLPLLPEPTVSSHLSATAASDGQSVGGGGDAASAGQPHRSVSSSDEDSAAAGRGSSGGGSLGAASLRGSTHSLVSILSEVERQNMQPAARPEAAAVTAVRENPNCGSSLAHACEGDAKVSSGAGRGPFNAVVTRVAPAPSSPSGMAQVPRLPLQELQHQSLGGIVTSPRMAGPKAAGRYAAVTCNIPPGVPATAAAAQQQHIRIASAAAAAMPGPVRISQPEEPLTASLSLLQLQGLQQLRQLDQMQQLQQLPQPPRQLVLPGSSLQQQQQQQLLLQLLQVSGEIQAAALLLLQQQLQEQRQLTQRLQVQELPQPAQQLQSGLQPGQQLHRHHHHRQEILSPTQQLCQGLQLMQHQQLPVPQTTAASLVIGTDQTTSQPPSSSQPPPGDAVSTDFITATTTSAHENRMETSVDGCARETFPVQPHVQSQAQEPHPEAGPGQARAAMVPGRRAASVDHDQSNVTFHPHLPAASAMEVRIAPPSSAVSGQGTGGTGRARVRGNGGRCTGGLELAGRPQRMEDKADGCPDGSSPQRGKCYRRGRWVCRSTPPRHPISSSSSSSGSSPSSPTLPSSPSLQSLSLGMASETVSGSGASRDGFPGFHRHKEGAQHRQSRRSRSRSRRSHSHSCKWRYRDRQHSQSPRMGRLGQSWDALRPHLPPPPGWMSAAPHRMGVSRLDSKQGQHRRAGVAPKDAEDGVSPAAVIESGTNANAAGRNGWRWFQAPGARAQRGELGYTVPHSETDPASERRSAPDQRGAPPAVDGECVDGEWNRSNLLQHGVAGTGIGAIIHSHGGGGNGDDLNAYDTAQGVSRPPPQSAISYLPLAIDAGAEALRHVLLDRQAGLPIAAYGLKLRDAVHLVVGSIVNSRHRSPAEVVAPPQSLPPQPASRIHAGSNPLFGGLPPTTITTAAATAASVTPTGNPRVAGISGCNIPLVRAAAAANAGLASSGYQGRSSKAATAIQASQYVTTSGGSVKDDAVNAKGSRTPPPLPSKCQSLPYVPDIGGDPTSSQPRMSGFDFVSVAAAAAAAATAAVMAAASPKQSTGRPGVLAGQMGEQLADEKEQLPGPPIGPLQQSPAWRGSAGGEAGLARSPFLLPIPKDLHRGLPGTTFTTGPVDSDTANLDLPSAGYIARAGNSACQQNHQDRALPEQRHLQCRAICIPRAPSRQQPRCVPQPVVHRLVKMRMQQQAGHNHECSTPQERQSPPASPHYHHQWAQRRQRRRRMLASPRSHTANILQVGESSTATPPSSSACRVPERFGAQGQGLLGLAGLAAIDQRQGTSVHVDMPNAISNCPLSVNEGRIAAEESWLLGSTHINNPFNVRDRERGALRHHQMLLLRPTASPEIGRLPLVQQTNLSPPPWQQQPPQHAPQEAQAHQQMESQKQPQQPQRQPLSPLSRAHISKHKLPFTWPQPPTSLPPTDLQQQSGSLATGVIVPWVRILSRATRLTRARRAVALAAQAARFNAEDQQDAKDAVGLLTSSLPSPQPSTSVHGGNSDYNAWSVDGDYCTIPEYPPTGRLHLHRSGRGSRALDCSTGFSSTTSLLTSAGHFPDNSSSHAVSFESVARAVKTAELVALGAVTATAMSTRSSSSRMTSLGNAGSVITLSPRALQNSNAESQQSRDSIKGKQQQQKLHKPPLALPLQQRLDLWPSSSVSDACVVDADARTATVDNLQIIRSPQQFHMDNLSRGERPSSDESSTVIAEQWPGISGTTPDPPSARNVRVPGLLRPSAGSSFRKQSDDGGGSGTMQSPDTIDLLGFLRPPTLASEPAGEGSDGGIGAITAFETVVLGAAHPSTAESWMFASPSGNHTARLFNIYNGRGWARGGGGDAAITRARYSPRRCSSSQAQTEEALYSSNSSMNFCTGVAGGPSFSSGGYTHGDYLSADFASSCVDNTRSRSETYGTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN09</accession>
    <name>A0ABQ5SN09_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IMP-specific 5'-nucleotidase 1</fullName>
            <ecNumber evidence="4">3.1.3.99</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_016137</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>IMP + H2O = inosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27718"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="3.1.3.99"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.99" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009453">
        <property type="entry name" value="ISN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213:SF1">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06437">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI71021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="480" mass="54288" checksum="5CD1B50AE0A4EBCB" modified="2025-10-08" version="1">MIQRVRTKSRDTRTGRQCGAAGVRPAAASRLRDVRVASAAPSAANPQTVSFHPHIPPTPQSSAPTNSVNGTAPSTVAEVNLGYWELLNNPESGEVFSQRARSASDAAVLRRKGHLKEQDRLIDFMRRMHETHTCEEVMLKMERWIAEHRQDPKRSRLRRLIPGIGAFFTPLKLVEAFREYDAFFALSRRKYIPPNFAELRHVVNISQVHASADTLKLITFDADGTLYADGAHMEQDNQMIAHIINLMRSNVQVAIVTAAGYPDEPEKFEKRVMGLLEAFRKLKLPPEVTNRFHIMGGECNYLLRVRPADKRLEFVPDNDWKTPIMRSWREEDITTLLDEAEAMLQEGAKRLNLVVNIIRKSRAVGIVPTQPTIYEVLEDIALTIQTQLVAKVPFCAFNGGNDVFVDVGNKLLGLEALMKYLRVTPPEVLHVGDRFTDSGNDVATRDICSVLWVANPEETGFFIKMLLKDIRKSRWQPYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNS2</accession>
    <name>A0ABQ5SNS2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016938</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03571">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF45">
        <property type="entry name" value="CLATHRIN INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="4">
        <location>
            <begin position="18"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="211"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="334"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="462"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="462"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="811" mass="81094" checksum="7D153DD5EFC5EB16" modified="2025-10-08" version="1">MNINFNSVLNTLAAKAKALALNVTDIELKVLEATNEEPWGPHGKDMQEIARAAEDPEKYNLIMNVISERLQMRDENWRLCYKGLLLMEYMVKHGPWRVVEELARSVSSLERLRDDFEYKDAQGRDHGINVRQRAGELAALVCNTERVRQEREKAARNANKYKGVSSSDVRGGFGGLGSTNYSSSSGGGGGGGGGYGYSGFGGGGFGGGTGGFQGQGFGTTESVHKPNSLHNRTGSGSNHSAGTGPSGRREEGGGFRTAGGGAEEAEDPFEATRKRIERLKAEGALQEPPAAGAPPGLADPASASAKIPKKLSDVKINPAVAATFASISIPSSSSAGNLVKLAPPPSSKPSASSQQQQYNDNNNDNSAGIGSLDLFAEQPSISQTTQGSLQHQGTSATSAGLDPWNAAFGLVESSAGSALGAAAAQDPFADFASAAPAAVAAPAAASSAGFDILLGGGVSSTGSGTTSTGATGGTNSSAPAPPAKQPVRAALPFDAFSELSDPSPAPIAGHMSTGLSARPATPNAVSSPDPFAAIASSSASLAPPLGAVAFRPAPAADVTASDPFSALSASRAAPPAPVMATLSAAKPAPAPAPAPAVCGPLPFDLFASPAATPPVRPPQPAAPAAPASDPFAALAASSTPAPVPMVAFARPAAQPQHVLPAAAAPAPFDPFSSAPAQPPVLSRPAAATPPFGAMAATPPPPAAPAAGSFDDFFGTSSPGQNGHFVAAPKPPGVTTAAATSATGDPFTTSAFTAFARPPPLPQQQPTATVSPAIATAHAVSTIGTQMAMNKSGAAHGAAGHKAHDPFAGLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP85</accession>
    <name>A0ABQ5SP85_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">P-type ATPase A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_016655</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF19">
        <property type="entry name" value="COPPER-TRANSPORTING ATPASE PAA2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="311"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="9">
        <location>
            <begin position="132"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="435" mass="43831" checksum="01104EAED4118B9C" modified="2025-10-08" version="1" fragment="single">MIRLPKPQRTSCVFQDYLPFVLPLGMPLMTRCAIIHSPPCGCCCSPAAAGPLLTADASFLEEPVMLLAFVLLGRALEARAKVQAAADLRSLARLIPATARLVLDPGVAPGAAAASGAAVAGKAGAGPAVEYLEVPTNSVRAGDILRILPGEKVPVDGEVLSGECCCDESLLSGESALRAKGPGSRLVGGTVAYEGAVTMKATATGARSTLSGIARLVSDAQAREAPVQRLADTVAGRFCFGVMTAAAATFLFWLTAGPQLFPQVLDPDLAGSGWAAVEASYHSHGAGGWGGGAAAAALDDDSLLSTSPLLLALRLAVDVLVVACPCALGLATPTAVLVASSLGARRGLLVRGGDVLERLAAVDTVVLDKTGTLTEGKLKVLGVQVALTDAALEAGSSGSGNSNSCDSPEDAVLRLAAAVESATRHPLADAVLAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPC5</accession>
    <name>A0ABQ5SPC5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016406</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047282848" description="Guanylate cyclase domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="592"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="4">
        <location>
            <begin position="639"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="890"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1488"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1572"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="894"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1497"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1509"/>
            <end position="1529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1598" mass="168967" checksum="8A7EF420E9854D47" modified="2025-10-08" version="1">MRNNVWGSLNALLRWLLMAICCVSAAPGGMAVSQDKLTSCVADFVQDIDGLYNFSSGLIGSNATIIRQQELTKAAVNFSLGCLGLQVDVARAVRIMVPMTGMVALLQRHADEFEAATGVRVILNPVLFYEVGKEVLQELTAGISDFSDGWVIDPAITGQVASLNGFKSLDDYIPEDASLQWNDISRFFRTASAIYDGKVVAIPLDGDLMLMYYRRDLFLRYNLSVPVTWDEFVQLAEQMNGTDTDGDGVGDLYGACLDIHPSICKANFILIAMAAPYIQYRGTAQGVFYDPDTMEPLFNNAAMARAFELYVRLFAVQPPTPSCDASNAAFRNRTCLLTINWGDEFRANAGGATYHDGLGADLGVAALPGATEVLDRPTGRMVPCTSRYICPYAIYTRLANGTFAFVNFAPYSAFGGWAGAISTAAPSEYQKKAYKFFSYVANSKNSWHDVSDPRSGIDPYRRSQLAPDPENLDRWRAAGFDLLPTRSYLATVNDALESGNVVLDLRLQSSVSPRLLLEAALRNLTWNRTANPNLTVPVVLGRIQTQLADGLVGSMPLTELRRQYCKLIGCVVQQPEKQVPRQPEDSTSHSQLIPLFITVPVGIVLLALAVVLLMWWRHAHRRNLFSGGVKAPGAGPATTLLITDIESSTALWELLPEHVMGNAIEMHHRIVRRALSQYAGYESATEGDSFICAFHTASEAVQCALHIQQELLTATWPPELLACQECGAASEVYLTPSAVHSEVMDMLVPYKSVQQPARPGEPGYRDNATHHRQFGLRILRKRLWSRTSGGGGGGGTSGSVGRTAATADDGISRHFSEGAIPVPDSGPSRPIGGPGQFTSPSYGLELAATGYNRLGGGGGSGGLLSVEGPQPAGDSSSGLPSAVYQRLTSMSSRLGGGGGGGGGEGSYRPPSDTAFPREAPLSIATAPLPAVVMAAPAPAEIVSWPMQRAGSRALPYSMPSGQLTGSIAAIEESTTGVSVSGATRTVGSARVLQDCIVAAWRLAAEAFPVIHIQLGSSRGAGGPGGGGAAAAGAGGLDSGTATAAPLSSSPPTGGSAAAAAAAATAARRATWRRGRTINRTASVCAFRGLRVRIGMASGVPEPSDVSYNAAEARFHYNGIGMRLTRAVQGAAAGGMVLLSDSTFAQQRQEQAAAHIADAADAAASGGSISNSGSSSLSGVLWLHMGQHLLHPDLAPQQVYQAVSPGLSGRLALFPPITARRCFAAGVLSAPVRRAAIAFVHVVGASSLLAWDAAVARTALKLLEEDFAAKLCQSYSWTDGGVEGNTAPAEADGSGQDGCWDGGCRGYIVEAYGGLLLASFSHPGQAVRCCLALLDAMPGLPWPAALLENALCEELTVARLDSTGAVSREVLFRGLRIKAGLDYGAVHATINSATGRVSYRGRVMNRASRISSNASSGQVLCSRDLYDAAAALAFATHGSSEQLPFSTISLGASALKGLPEPVELLLCRPWEPLSARHLPVRISVAVSEPSNSEQRLVPPLPPLQQPQPQPQQQQQQQRQRQLQTPAARLQESVSHRPVRRLSALLRMELQVAASPTTHAPISAASDAPLHVSTPRVSFHGLPGDPQGLLVPEDVEAEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPJ0</accession>
    <name>A0ABQ5SPJ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016568</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045670141" description="Ubiquitin-like domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1205" mass="136251" checksum="807D0DEC009C310C" modified="2025-10-08" version="1">MGSSVFVLITVLGVTIGRYCSTVSFTQSNHDERARQLFVVLPGSRTLTFSGSTDSTVGQLMDFVSSNSLFTFQPGSVWRLTSSRGDVLSDRDKHLGLYGLHNQYHVQVLGRLLGGSGRLPNKRPASSKRDNISDAWLQKHKFSRCQDPLGQEVYACTQCPFTGRTRSQLYGHMKRCRLSFPEDTNLNMDNENDFNTSDDHNEDHSSSSKDSSGHTSEVLSGDSSGHSQVQEETALPSSLVSALIHVMDDLDPANPNHRQTLEYISAILDNLCQHSYPESEDDEPDAAELGVLSQLGAMDHGQLQLLEPTNPAELLASGSTVQAYKERLDDPLWRVTVNNGDGVVVCQEARITVGQFGYAWLKEKMAGRVRDVVADRQLRFLRDICLPPGNHVPPSLYVLRKMLGIPDPRDFEQHICLSEKCRFPPLPTKDWMDHRDDTCNCPEKHKRFKMVTTSAGAHPLPNKRYWDFGVENIIQAMFSDPLFRQLRGTGRDNEPDDFYGSQMAKDIDAKTNGQLMQHANSAYDLGFDFAQVYQFKTHSVGLLLMRCTDLPVAHRSKRRFTYILTVIPGPEEPSSMDVYVSRTLDAFKEYGPYSESSLMVMGNDRQLFEHKIFLSAIFADTPANRKLSLWNSHTSATGCGHCLLKGVSGEGGGMYFPGYAVDADVNCIFDGVIPDMENAPLSARCGDPRIMVSHEMHLKRAALAMKDPQWTGIVGCKGLSPIIRELDYCNYNDVFVVPVAHAALFGVIKRFWNTILGKLGRHVMDYTILGPQRKIMAQRASHIITTNDLNRPYRDIVKHRGTWTMEDWLHWLDSYSVYILHEEDSLQSSTPLMPVVQRVVDGITKSFNIKVMWNNLRTALLHYFRYDPSDFSADANNHAAKLLKSFSIAAEHVFGIQFCTYNLHILVCRLREQENARGHVAFATEFWVERGMQMMKSIVKYRSPTVPELVLAGGYLASLWLTHLKAAHPETFRTFDEWVPGYRADNIQLNPSKRDVFLDEEDSEGSQLLGQGYIADAEQRSHILGGILRTEETEDFMARTNLLPSVLQDSDIYIFKRALKRRDEVVLSVDYARATKRQSYHFKCKYGPGGMVSESDPRVSIFIGEILFFALIRPRCDVAPVKVAVCNLHRAEVLRPKELYRVKDYSRRDATVNEVRQEILLNYFPKEVGMIDSKVIRCRVNNELSYFLTYITFSQLPTNDGGEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPP3</accession>
    <name>A0ABQ5SPP3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="938"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="753"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="906"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1159"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1543"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="753"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="779"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1198"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1208"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1240"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1567"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1630"/>
            <end position="1640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1640" mass="175002" checksum="8177523BA949989C" modified="2025-10-08" version="1">MASANECVMTNPAVDRNLGPADLSDNRPWLLFDLRFLVQAKYEKDQRQRQQQEAQQQCQRRFQSRHGITSCLLQLRAKFRLGIFTTEERRTVETALLGIVNEIQVQLKRGKQDQLRQLQHESAAYALFANDVTIGGPSCHGGGSGPKPTCGESDSNLAAATSPTGGIASIEGPDMDPAPDCHAVPARSRADGAGCRSRGSSIDGHPPSGRDSPIDARVFFSCILHRDNCVPDPNWKQKRHGKGGQPGMIKSLAGNNLPLERCLLLDTGTESSAPGEQANLMVMSAKTKFRVWQKLISLLMALPAGPDVDIRNHIPTISQRVAQVSGKKGDAAPRVLGAAKATVVPLPLAPEPPQHVSAAKLQALERILTGVLHQEKAVEKTLASEAAVEPASAAVGNNNGLASRVRQSFWVAAAETAAVEKFAAETAARRKVSANLVTAAYGNVPHVVVLGALDDAQQISIIRLLAQQMAAESGVKPSATKPLSPLQRQVVQQLQLQHKTKADPRIDTLIVSCSLAASFAVHGSVSYEQVLRPLTKEAGRMMAKEARIRLLRLAERLGLVRCSPPPCRYMDARFSVDPVLDPESVAAAALWHGRQAPKASSPPPHDGKKEEAVAGVAEADEGGAGSGSGNAPMDVAAVQTAGTSLPYSQLPLLLELRKRVWDQQHLDGADISEIIAIAKGLVSEPLRPLLTSLSMTRALQSLVTRGVLGKWTIPINGDRPIVKFYVRALPKEEHLREALEALELEILGGVDEPRQETRCPTAEHQKEFSKYSGSGSAEEQQRRRREEMEEEWAREQRQREWEQEQEEKERAQQGGECLPPTPTPLPTPLLTKLPTTADMTDSASAGAAALGGSESRKRVWINMAVAATAGGPRSSTLPGATPFYGRPWIWPTVNRSLIKPRPAHKACGLQEEQIPPQQRRESGGPGLVVSERRPLSLLHDEMVAFARDSSPGKEELTALTRGLAAIQRVAEEIWPSARVVLFGSQASGLALPSSDVDLVVLGTSRPTSRPSKDFKSSERTRVAHKLGELAVKLRRAGFIRSYEVIHAKVPIVKAVLLPLDENDRPMQNGGLHLAMDISMGAETGLTGVAMVQRAVALLPPLRPLCLVVKALLKETGLNEVFTGGLSSYCLVNMMIAHLMCEGFDTKGILVSSSPEHACPTRASTGHLTRVIGSPDSEATAKRPRRGSCADPDAEDAAGVDAGVGANMGDDDNYPGEAEPDWVGSDGDSGRTSGKGGDSGLNDDGEDDDDMELYIESAASGEDPVHGATAPCTHPHDDDDGIAVDARPPHIGVNSPVVSEERHVAPSGVALNGIAEDKLAESVMREAVEAAAEAIAVINGGAGSSSVSAGLSTAQVAYLRSLGRQDFSAVDLGELLYGFFIRFGRQLDLSREAVNIRQGGITVRGSTCGRGHKTAVTLATDDPQQPGSDLGRNSFNIAAVLHQFTAAADKLADLARAAPERLLAGEGDDSEVAQWAAVQGDGSHRSVKALVTRYQQFKILNRILDLDLALCRSEKMAATRVANHKKKIGTAARGRGAAYKKARAIKGGYQPGSNQRVGPARTQGLGVKKPKIKKNPKKGPTGRGKAPVGGARTGGSSDGSTKRGRRGSIWRGRADGSRIKGGRKASESQQGQGGRGGWVMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ37</accession>
    <name>A0ABQ5SQ37_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_016837</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45926">
        <property type="entry name" value="OSJNBA0053K19.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="5">
        <location>
            <begin position="18"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="140"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="260"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="498"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="584"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="720"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="783"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="920"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1123"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1284"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1432"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1520"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1581"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="828"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="270"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="340"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="600"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="636"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="660"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="729"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="786"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="924"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1139"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1183"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1203"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1222"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1301"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1432"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1532"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1581"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1615"/>
            <end position="1633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI71573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1633" mass="165557" checksum="915043C1E7D7CE5E" modified="2025-10-08" version="1">MDEETRSTLLRAVTAVMQREEAQKYFNEPLNSSEHSGYAICVQRPMDLGTIRRDLQLPPGESPYSSAAAVLQDVQLVWDNCAAYFPPGHEARAAADTLSGTFTQMYQAAQQKQQQLRMQSLQQQQQLQLQTQQAHFALQHNPHNQQQQQQAALGHPPPHPMQTPRQPFQHAPQPPQTFMFVPVDNAPPSQLGGHQPPPAPAAVSAPPPPPPQPPSGGAAAVTGSSAAYPGAAVMAPGPPPAGAAASNDAAARIVHPAGGPKLKISLPQHGGTGAGAGAGHGGASAFGSGPPHAQSHPSGLAAGSNGSGGGQQTAGGDNAVVVPAAESGGHSAQQNVGSDATGAPGPAIAGVPAAGAVSTTGSPNSPNSLRKRSAMGGLDAGTSHLTSNTPAKKARGPAVGAHGGTVKKEEDAEQLSTSGAVGPVGHSSVTVAAPAPAAPVSTAAGGSVTQMPGGRCDVCRRLKKGRCGTSTSSLKCEYRRLNNLPYADPITGERIIPPGYLASSEEDDEAAAAAHLHPHPGPSGGGGHPLRGGAAHSDGGASAGIPGGGHFTRGGAAALAAAAAAASSSPSLGADCDGATLASTLGGGGAPIKPVRINSGRPGPKPGARAAAREAAATNGSSPSLRGTAYGGGRTPRGATGTASGVSRAHSGNPKARQRSGNASAAALASAASGSGIVEPGTDRMQRPQSLVPDPVESEADLFLDPDEELALAAAAIFDSPPRDSCGANAGGTTGSGGGGGGGGGGRRSSGSLMIDDVLLGGLGVMLDGPLLDANDVGLSAVLQQRPPPQPQPQPEPQMKRRSSEDGASVCGAKLAGSGNATTKPQTVAQAQAALQAAQAALSSAQARLAELDRNWVVSEQQNTERKQKLAEARATKLKDLERLSITLRARQGNVEHARAMAARATELRQQLERTRTQLATMQGVSPSPAPATASGPNGANGDRTSQGGSALGLLNSSGGPAPAASAVGSAVNGIVGGGRGENSGAVGGIGIGKGSGGPRVTTANNLKAGSPPGGCCDASDFLLTDDGGTGAEPVPAAATVHCAGNSVAASSGLGICGGPGSGTGAASVATAASQGVPMHGTEALGGGSGGGVAVGNTRENGPIVIGRPVAGSCGGNVVGGAVNPTGPLPGDASLRRASSQPQTLPPVLLQQHHQQQQQPSQLDKQQTSAALQPLPHHRPEQQQHPQQQSVFPTASAASVSSGGLGITGGGGTTSMGGLGGQQQQQQQQQQQQPHQPHPHVSSAMAHLEAHSGQSTAQPGQVPDAARFASQIAGGGGGCVPTAVSAPGQASGDAVGTHGKQSAAPQLQPQHQQGQVAQQQQQQLVLQAHQQQLAFQQQHSLQQQQQQQLQQQVMQQQQMHLQQQQQQLLQQQQQQQHQTSNAQQQQQQQQQQQQALLQQHALHQQQQQHLLQHVQQMQQQQQQPNVNPLLQLPQQQQQQQQQQQQQSQAPPPPQQVLQYHQAAAAAAAAMQQQQQVLLQQQYYAKQQHLQMMHLQMLMQQQQHTMMRPTLQMPLPAAVLAQAAGKASPPQPQSQTHAQAQTHAHAQAQQQANHVAHGGQVTPAGAGAVMASVPNAAVVAQQQQQQQQGQQQQQGQGHTAQSQLAGGLMQEYGGMQTVTGPHSQMLIQPSAGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SR36</accession>
    <name>A0ABQ5SR36_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016791</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24203:SF45">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24203">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="34766" checksum="EF30843F549F4B67" modified="2025-10-08" version="1" fragment="single">MGCGPSSLAAAELNRSLRAGDGSHLLQDKLQTSPWLLSAATPVLSSTAGTPLHTACERNQLEVVKQMLCFLSSAPLPVVREALRPYCNRNDIALPMSVGEGLGIAVEMVNCKGQTPLMCACAAGSPDLVKLLLAQGADPWAGDRCGSRTALHYAAMSGRTACIEALMHDIPVRNRARQGVRYINVRSVCGLTALHYAIYFDHTSAVEELLRHDPLLSAVTSSNSYDVLVNCDPLSTPLHFAAIRGNVGIVRTLLRQYVQGPSSLRSREPRLRANHAGVLPWQIAVSYHPGNRDLVALLHPGEPLDVAMGLAAAGDESAAASVPGART</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLY5</accession>
    <name>A0ABQ5RLY5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000352</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15437">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15437:SF6">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="582"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="710"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="853"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="901"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1028"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="745"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="914"/>
            <end position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1053" mass="114505" checksum="2A04D2FA87BADCFB" modified="2025-10-08" version="1">MQVCWNRPLPAPAAYLRLSGSPRRRKSLRQVCQQTWERPQSLIHCGILPDGYLGSHLEWPVLAYGAPRLADLAWRQPGPYGRSPIATFARLVRRLNNCTAPLALWCHSDHSTSAHGNVKMVFPDLYSGRITPLKLARLSEVLRLPVMRVETILQRSPAAVRHMEPEHVAHRLKELCLALYILPKQAAFMVSKQPAYMLLTPLSEIHAKIQQLSNTLGVPPTAVLRTALTVPSILRRSTAFTERRVKAYSTLLRCTPLDVLHIMARGPEYLSDTPSSVRQRMTALGYVLRRPQCTIVAMLQARPDLAFMSGKVMNTKVNGLMCIMDKQKHHVVTLAVKLPALLRCNLDNVRHAFGGLQELLQKREGFVYAMICHAPRLLLLTPRSLHQRCAALQRCLATSPEWREQFTQLRPPGVAQLVLNRRGSLSAMMYLTQRRRAGTAHLAQLLSSSPDGGLVKVLGEGWPDFLEWMDRRRQYITYRMRQHRATMRMAAEGVARAGPAALQMSVNGSVPQLQVPLPPLKQEERGMLQRRGQHQKSLSPVAAVTSQVLSGGAAAATAIGIHLYHEAGGLEQHAQQGIVGSPHLTLQPRPADSSLKPSEDCSCSNRDHLHIKSNNLSSRDDECSLESISQQDAGAYVAAGERQQSGASTSGRQLSWLQLQAEYRRSLQSRARRKAASAFQQLARTETEAPPGIEALPPMKRIRSIRRTAQMKGTNPPMPISASEGLPASPDERGRPLTGWDGSGKASTQRSQQLAGASPALEPSSPHVQPQNASWAVAFVAAGVGATGVSELRAAHGNEPWEALHTADAALCNRDLDRLKELTCLHPIGDGRQSHNHDLSTLLSLGRLQQPNPGLAQAKLGNNGATTTDSSDGLDLEGEAFGATLTASCAAQLTQHVGSCFKRDPENTAGAGETMSTEGNGAGSSWPSEASTQAVGPHHVAEQGARQMPLWLHLEPSGIRRNVSDQGDLLGRAHQGESEVASCRRGAVPPAEVPQHGPTDEVVLVSGGGSICDIRSLGRDTHALNARGQETAGGVSIGQKQKRESTLGAIMPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMJ2</accession>
    <name>A0ABQ5RMJ2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="463"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="487"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="568" mass="58516" checksum="0FB77D8E6407131B" modified="2025-10-08" version="1" fragment="single">SGLEEGSAAVLVLPARSQPAQPQSTSDPRVQAAVLLYTPIAIAPPMVASELGSLMHEMYRTSAAEDCPTDRRIIRAQAFTHHFSALISDIVSLLLGCERLADEAEDLATAADASLPGGVAAPGEPHRAVVLERNQEELQAQGIRLVSYLMEMGLKGTARYLLGEVQNAGMQVDIGESEGAEAVCEVLGLGQNVAPACALSPCASAGEARVVGGGGSWVAEGSGPAGREAGDHSAAGGDSDQQDQTHPHGQDAGGERGRPLAPTNGDRGSDSGPAAQNNTSSRNRVGSHSLVGAPRHTGAAAARLRDVLLGFNALEREHGFQVFLGGRRAVWAQRLISSFVLAAVLPLGLAVCAVLAGHNIVNDQDVSCDNGTSEGPPAVVEVAIGGGDAGSWEANSVGTSVGCRYWAEVGDDTAASQILQRLAGGLQGVLVGLGLAAGVLALPGAMNARDEDGAKVLRRRETLVWIIHLAAVVLLVGFVGVSPETPIAGSLLWAVCAAAALVVVLQPCIVTVRFLSLRFCWTVDILAMVATGLIRWHTGAQGRRHGPERDGCSNLVHSKGTSACTIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP48</accession>
    <name>A0ABQ5RP48_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">STEEP1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1200.10">
        <property type="entry name" value="YggU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057965">
        <property type="entry name" value="STEEP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053323">
        <property type="entry name" value="UPF0235"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036591">
        <property type="entry name" value="YggU-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47525">
        <property type="entry name" value="OS07G0295200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47525:SF1">
        <property type="entry name" value="OS07G0295200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25809">
        <property type="entry name" value="STEEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69786">
        <property type="entry name" value="YggU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STEEP1" evidence="1">
        <location>
            <begin position="19"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="227" mass="26023" checksum="D422E6E324CDD43A" modified="2025-10-08" version="1" fragment="single">MPKRTTLTFTSEDGPRVDSTKLFVYHCKYSGRHAFTIDADLKKMPRRRTDGAYVIDTDEHTVKLYTADGGVKFIKRQNGTVEKQYRLNLGQLPIAYKSDLNSNLLYIMDGAVTTYSNQNARRAGRLLVPPCISRSERSGLVEMRLELEDRSHRCMLSRVTADVVRVHVTGLMANDAVHEELFDLFAKVLNVRLSQLDIRRAKSSRNRIMTVESLTPEQIYERLREAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPF5</accession>
    <name>A0ABQ5RPF5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000712</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007811">
        <property type="entry name" value="RPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05132">
        <property type="entry name" value="RNA_pol_Rpc4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="285"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI58927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="449" mass="47816" checksum="BB6FA3823F986227" modified="2025-10-08" version="1">MGSPLYAPRQHQIFLSPQSALLMVLQPYYWETVDARSSRAMSPYSLSVSECLTWDTSQSHFMHMMHTTQFMPTMPGRRKKAEEGGAAAAIDAATADETFKDLIKQAQSAAKTWGRGRGRGRNQQQRFQVTFGVGGEERPALAAAKQYGRSGGGGGGVVGGGGGGRSAGSRRVKSDGAACVDDEALPDLSDSKQDFKSIKPRPPVLDYSQYYPTILPLRVPGEEIWEEADGGPAFQPSALDLAAMPDVLQHPAEDLDLFDPEMEDQMFLMQMPGVLPLKPGGAGAVAAGDGGGGGAGPGPSSERRRKEAAAAAANKAPGCTMRDIPSGRIGKLLVFRSGKVKMQVGDVLMDVSVGLPNQHRQDVVAINSSSNACVFLGDVTQRVLVSPDVLQLLRESSVPEFARAPEELTPLMQAHRRARGYDLQEQLQQDVSAAAAAVVSGVRIKGETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPH0</accession>
    <name>A0ABQ5RPH0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7 10">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_000730</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6 10">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7 10"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI58942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="263" mass="28168" checksum="7073C35C94964F88" modified="2025-10-08" version="1">MALNMRSSLKAVGARAAKPSRNVVRVQATHRVDQCKKTDIIVSPSILSADFARLGEEVRAIDQAGCEWVHVDVMDGRFVPNITIGPLIVEALRPVTDRVLDVHLMIVEPELRIPDFAKAGADIISVHAEQSSTIHLHRTLNQVKDLGCKAGVVLNPGTSLSVIEEVLDVADLVLIMSVNPGFGGQKFIESQVAKIRALKAMCLEKGVNPWIEVDGGVTPENAYKVIDAGANAIVAGSAVFKAKSYRDAIQGIKVSKAPAKVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPV9</accession>
    <name>A0ABQ5RPV9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="188" mass="21283" checksum="0B1C1B5F4B556795" modified="2025-10-08" version="1" fragment="single">VDPKLLLFNIPVRDLRMATPMQRAVLIVGAASGLGFGGYYFSQLQDVQKYEKDKKDIERLIETERKRLTTTAQAQAEQESRISEAESQVRERQKTIKDLELNLDAARKAVQQLEQQLKGKNEDLQSKQKELQSAQSRLADLRSETERAKQSVTMGEKSLLLANQKVAEAKLLTNPLNHPKVKTLLGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPY6</accession>
    <name>A0ABQ5RPY6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001551</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="2">
        <location>
            <begin position="85"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="584"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="392"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="592"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="670" mass="72172" checksum="CE43863049614386" modified="2025-10-08" version="1" fragment="single">SVCVTALKTLHFSCCCCPCIFCKVTGESAERRSAIAPPACGVSVLPSVCLRSSAMQTRRIGGLRYFTKGELEATNPSRRDGIDAITEAKWRSTTSKIVKLTIKTLKLPDWVYETAMCYIIRFFLTRSISKNDRHLVIGGAILLASKAQESPRPVQDVAYVLLQLKYNTSQKSQTGGHQITLEQFIDGIKMAEQAILFSMNFHLNVETNVSLARGLLEPLDMWAKTNPLAEEAEDNKLKLSLYGAMMFFLNDSAMTTLSVQYPNSKIAPVALLMAAKRIAETRYACRDIPAILQRVLALGDDQAWLDAKGLSLEIVTDISEQINELYNSTPPQAAAQAQQQRQQHVPAFQGSAPTAAASGKDGDATHQNLASSALAAATTSTPLCWGRGQPSSSAAPGTIADAAAGDTPELPHTRSPRCKLESHPHQLLHAGTRVSDGDECTGDEQQATSELHAAPEQRPEAEEDVCACNRNAAGSGPCRTCTGLEDSANENCSPDGLTTGNVSGSGCRAAEGQPEVCSRTETRLQQLQSQPTQPPPSLEVSNPLQSQPSQHEKRGREDNVSEFSGSVPDDVDLIAPLYKARRLDDSQPVSDAQISQPQSQPTQAQSTPAIPTSTSSQVQSHPSQCSKRVREDDVATEFLASARVMSGIFASAAKVRRLEESQAVPVWQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQB1</accession>
    <name>A0ABQ5RQB1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(P)H dehydrogenase (quinone)</fullName>
            <ecNumber evidence="2">1.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001670</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a quinone + NADPH + H(+) = a quinol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WrbA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.2" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="6">
        <location>
            <begin position="68"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="265" mass="27770" checksum="D9F69C9A8E3F6AA7" modified="2025-10-08" version="1">MGNVCGSRAEAVDAPTAAKPTATVEPTEPAAPAVTTDATTVKPEAPGDETEAASPAAEVSNPPKKLKIFLIFYSTYGHIYKLAETYKAALETLEDIEVSIFQVAETLPKDVLEKMHAPPKPDIPLADPHRLPEADALVFGFPTRFGLMCSQMKNFFDATGMLWQKGALHGKPVSLFTSTSTQGGGQETTIMTAVTQLAHHGMIFVPAGYAAGTIMFGVEHAKGGSPWGAGTLAGADGSRQPSEGELEAVCVQAKAFGQIAKKLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQE7</accession>
    <name>A0ABQ5RQE7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001103</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the Hakai family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040383">
        <property type="entry name" value="HAKAI/CBLL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13480:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HAKAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13480">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HAKAI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="114"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="38695" checksum="9B25AC06A4EA1457" modified="2025-10-08" version="1">MTAPPLPYLGFSYEPSPRHKTHFCVSCQFPIAIYARLYPCLHTYCLACATDMATCFLCQANIARLERIPKSRDHKVFISPLTLQSFKSEEDLSTHTRRAHQQLLSSDVIRNAGRPKQEVAAQAQHAQQLQSSSQPSQLQQLPQPPPPQQQPQRQAPPPPPQQQQQQQPPHQHQQHHVHQQAKQHGQNPAPAAPPPLRMDHAIQAAQQQQQQQMAQQQQHFPPPPRVPPPQPPQLPPPPMQSAVGQSGVQQQQQLAMQMQGMAAGMGPQQAAAAAAIMNALAGGGGQHMMPQAAAAAAAALGMQGMLGMQGMQGMQGPMGMQGMQGMQGMQGPMGMQGAMGMQGMGGLPGGMPMPFPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQF6</accession>
    <name>A0ABQ5RQF6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001440</name>
        <name evidence="3" type="ORF">VaNZ11_001444</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="2 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="156" mass="16642" checksum="C9684EE151628EA2" modified="2025-10-08" version="1" fragment="single">VNTNMDDAADPRGLPLPQTQGHAAIPEAQDPLGEAVALDQELGATRPASVVTAPRSNRRHKVRGGASSGVHGSGRAGPSSDPAKSRKRGGVPLTAAVQHSDPDAKRTHTDDPLMPKWNCNRSEFERRKAANVCLRCVESHPTKDCPLLPPFVPRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQI2</accession>
    <name>A0ABQ5RQI2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="7">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_001401</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF184">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0274">FAD</keyword>
    <keyword evidence="3 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="9">
        <location>
            <begin position="46"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI59509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="283" mass="31602" checksum="96596A8FD43ED76E" modified="2025-10-08" version="1">MSMNILVTVVERNWFLLLPVVVLVLALATVLILNRPKAKKPFLNPSEFKPLPLTEKTYITHNTVRLRFALPDPNQRLGLPIGQHITFLAKGEDGNNIYRPYTPVSDDDQMGAVDFIIKIYPQGKMSQVIAKMQLGDTMLMKGPKGRFTYNPNMVNHIGMLAGGTGITPMFQVLNAILKDSKDRTRVTLLYGNLTEDDILLRKELNQLVAMHGDRLSVYHVLNSPPTSGWQGGRGFITKGMIAEHFPSPGMGHMVLRCGPTPMMTAMKQHLDALGFAEESQFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRK4</accession>
    <name>A0ABQ5RRK4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002278</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="929"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="965"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="367"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="505"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="730"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="869"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1078"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1373"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1647"/>
            <end position="1679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1739"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="173"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="395"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="783"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1078"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1762"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1776" mass="179787" checksum="B944297D2B71D0D2" modified="2025-10-08" version="1" fragment="single">MPGPPPAPAGLHSALSAAMSAAARDAVSRHALHHGPPYSKQLQQPFPLSRTRSSGAVLVPAPASTNPSSEDPANSTYPGSPWTPPPTNSDGVDLLIGQPPLDWDTDNPQGYRGPLSGSDVSDNEGNGAEEEMGTDGIEGNVMAGSSSGGGSGDGATAGEKQSEADVHSNADGTDADANVEGDPEGADDGRSWSSRYFSRDPTLEGVIADGSPAGDAGNGGGVVIPVARGQPAVEDAEVLDAEDNLYMEAEGPGALPYLPTGPVDPQVIPSKLPQQPLPSAGAAAVRDGAAAPAIRGPAGGNGIDLGRASAATAGLAGGAAAGPSPDTVVAIGHGSGVSGVITNVKHSMSGGVTGIAILPHANIPGGYSGTNPSPATGARPSRPSWAGSHDSDPPSTSGSAYGAASTGLLGTTSGGRSGGGGVSGRRALASTAERSFQGGGIAASSSSRPQSMVDLELGLVGSGVVTTGSGQAPGVQSGFFSPDRPAPLKIQIEVSAASRLELKVRGRGRPATTTAAMPPPPASSRPLRRGRPTAAATTANSATTATANNGTSVSFDQPDGSSSTSSLASGTAVDAASRSFQSSALWSSARRLRISLHRLHQQYQNYHALQGGQDPGPLDASASLAAVARLRISGHGHEAAAAPGGTGGAPMSAPEDTVADVGGGVSLEHGRVHQRSVSAPSCRPQLGMSGLRMQTDTHLQLDSESSRGLIPSTELDKTLEVGWSDLLQHTDDDGAIQPPHAARSLPVSPRLAKGRAAVGSPRRTRSAGGGAAEEALTNRHRSASTGLSPTASPLTAAVASGSAVTAASAAATKSSRSSSRVGRQSLAHPVLIMPSMAATSAAEDPDFVQVVEKARRRAAVEKQKLRAAREVAAGDGNNNKGSMRRRGGTSAPRTASEKVRAAELLFQRQAEEWARTHRQLPPRRRLAPSVTRMVEKWFSLVDYDGSGHLETKELALALQAAGVPCSESDVVELVSTIDVNRDGAVSLTEFLSFLLKELGAGKNLTTGDYLLPSGGQLPFAAMIARLKRLRVLDDVMKGGKARNRYVDMLYNRDALAAEIGLSEEVAAGLQARAVPLESSNGEDFSETASAGGGGRRGGRVRLQQLFQQYRQAHQHATSDGAEFTGPGPAAAPEACDRDFDWEGARKEDGRQLGGERSEVCFAGVDGEEFGDVNITMRDPAPAAVIAAEAEAAAAGAEGEGEGGSHECDSEYDAVAAGFGEKVSGVSYYPRYSREVSVLQGACSSQRASNDGVVVLAPAPIPVVDQPMFFKLTEVLLPVAESSAISERLGSEVCGGGGEASSFSAAAAKAVEVATATTPQRLLLPVISPLPPVKRPNTRAFKRALLDVRHSAPEYLMQRLGLAGPLEAAAAAAATAGSMSPTSDRPRGRRRTGGDNGGGAAAAGRATASFRPLASTSPDGLVAGGEFSATSVSALTSASDPVTASAAASKAAAAAAGAYFTTYGAGFTRYKRWSMYDIDPASENPDYWQAKAAALLMRRTSRAVRGVPGAGVATMSAAAAAAVAAIAAADAAKDIELYPAVPVAAATAVMVEGTEQSNNNSGKQLPPAASLLRRHHNPSQSSQSDGAAVGWHLPSLQTGLTAGGAAAAPHITLQQPGATAPLAAATPAAAAPAASVASHVSALVPCPASPWRRTADEPASPGSPTRRKARSGPHGRGLGACALGRGSAAAAMLSPRAAPPATAASAAAAAGSGAGNSPIQYVIRHQQGANPRLAAEALRRRPVPSAVADGDPLMQRLLSKSASRSPQRSPSRSPQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSA8</accession>
    <name>A0ABQ5RSA8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002657</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046929170" evidence="5">
        <location>
            <begin position="24"/>
            <end position="2014"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="7">
        <location>
            <begin position="24"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="620"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="751"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="879"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="1004"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="1134"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="1769"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="1901"/>
            <end position="2014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="79"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="150"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="250"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1247"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1247"/>
            <end position="1705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1713"/>
            <end position="1760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2014" mass="212317" checksum="5600F89D666FDC6F" modified="2025-10-08" version="1">MMAEYQALLLLALVLPSVLLVHTKEQTQSCEPCSELDFEPVCTTTGLTLPSACFASCQGLNVSTVGVCDDSDPLAGRLTPQFGADGGGSTTGTGGTSAQTRRAMSSKRTSSEYGNDDNGGGGDGGGGTSSSIDDVVTRYVHEGFSYTGHVKITTSGPSAKRSTYYVSSVRPPRSAAASAVSSRGGNDCGNSTSKSSSTSSSCTGQGQGPQPTGRRLKIRAVRVSTDGAVYMQLVPLEVDVSNLPVSGKTETKVDNGFSSSNAPASGASTTTTINGEAGSRRRQLHLQEQQLQDRWSYPLRSYLPLLADDAEASGSGYGFGETNANNRDVELGGGVGGYSHDSHSGSSQRRRKTLEMLLPRQLQQMRQQQQRRRRLIDDSYRADVRLWPHSVIGQLSFRSNNVRYLCTGTWVTPYDVLTAAHCVFDIGTSVESRDFIFEPGKIGNTIPLGSYNHVHFTFYRVEYPSSDGDGVNYFDVALIRMRAPSSSFMGLKYSCAQIDYPKTQTCGYIQDNGNFQQCDDCYYTSNGCQPGVQPINWCYTVGGQSGAPIYDLTDHHILGVLSGGLTDPLKSDYSFWTPIDALHFTSLTRWMWQPGDDPQQLAPPPEPLTYACSDAESGAVRLADGPNAAAGRAEICLIGTWGFICSGGRKSYSNAAVVCRQLGYTSGGIMTSVLYPPNDDQIAWLDPACEGTEESLGDCPFQQGSSPSGDVHFVYCDDDLEVSCWDEQGADVTSAAATGMDLYPCTKEGAVRLRDSSSVDKGRVEYCTGGQWGSICHDGWDDLDATVVCRQLGYQYGIALKGVIANGASPPGPEGMVIWLHQVNCSGSETSLKTCPILGSLGTPTCSHRADAGAACSNTRRTTPGVPYVPITGCDSGALRLIGPYASAANVSMGRLEVCYMGQWGAVCEPGFEDLDAAVACRQLGFKAGRVVTADPLPDQPIWMSAVDCSLPTIIRDVWPLRLTTCGFWGMEDTGCSYGAVAGVQCTNGNFTSTAQSCSEDYELRLRGGTTNSTGRLEVCRRGRWGTFCRYGFSDRAAAVACRQLGFGTGYVLAQGIAPRGDPDSITWNATISCAGNESSLRECTQLASPSNLGKCNTHDNDVGIQCQSSPRPPPPPVDIKATGTFACDNPGQVRLMDSIGRLAYDRGILQICLNGRWGTVCNRGFGLDDARVACRHFGFVSGLVVASSLPANYNVLTAKQPINMFNLSCTGKEDRLTLCAYDMYDSKCTHAMDVSLRCLWDELPPPPSPPSPRLPPRRAPTSKQPPSPAPIRPPSLTPPNPRFPLPLPKPTLPSPLIPSSPPPLTLPPPPPSPHPPQLPPPPPPPPSPLPPPPLTPSSPPPRPPPPTLPASPPLPSPLPPSSPQPPPPFPPPPEVPLPPPPPPPPPPSPLSPPPPPPSPPPPPPPSPRPPPLLPPSPTPPNVPLPPPPPPPPPPPAPPPAPPPAPPPAPPPAPPLTPPSPPPPPPSPPPPPPPSPRPPPLLPPSPTPPNVPLPPPPPSPPPPPAPPPAPPPAPPLTPPSPPPPPPSPPPPTPPQPPTPPTPPSPHPPPLPPWPPTPPNVPLPPTPPPPPPPPTPPLTPSSPSPPPPPPPPSPPPPPPPPPPPAPSSPSPPPPSSPPRAPSLPSPPPPPPPSPQPLPLLPSPPPPPDVPLPPQPPPPPPPSPPPPPPPPPPPPALPPPPPPQVHPPPPPPSPPPPTPPRPLPPSPVVRQPPSYRSPPSPRPPPSPKSPPPPQLRPPPLRPKPPPPARPPPPPPSPAPPLLEPCGLEGGIRLAGSVADGAGMLQFCGDDGTGFLKWGSVCGRPLRPEVAMMVCKVLGYKDGSPITDPMIPFYGYDGMYYSFQPNPPNMPIWLHNMVCAATPNFMGDCWSSTSRCTHRADVAVACGGPKPIKQYGCGGKAGAVRLVGGRSRQEGTVQVCRSVVWGTICDTDWDDTDARLVCRLMGFPNGAAVSSEGRLMNGTVYGNPFPQGPSDMKVLYSNLDCQATDETLDDCVRRAQECKYNGSRRQDAGLVCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSH9</accession>
    <name>A0ABQ5RSH9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="279" mass="32115" checksum="8C39AB923CDA0D0A" modified="2025-10-08" version="1" fragment="single">VKRQVTDLLAKGMIRPSTSPYSAPILFVGKKDGTLRMCIDYRGLNANTVKNRYPLPRVDDLLDKLKGSAYFSSIDLQQGYNQIRIAASDIPKTAFRTPFGHFEYTVLSFGLTNAPATFQAVMDRMFRPFIDRFVVCYLDDILVYSKTREEHLEHLRLVLDVMRREQLFAKRAKCFWAQPQVEYLGHIVSATGVKMDPRKVAVVRDWPAPQNLTELRKFLGLTNYFRKFIEKYSIIAAPLTNLTRKGVFVSLESWTSQCQRAFEELKRIVAEDVTLAFPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSR9</accession>
    <name>A0ABQ5RSR9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F5/8 type C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051923">
        <property type="entry name" value="Glycosyl_Hydrolase_39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12631">
        <property type="entry name" value="ALPHA-L-IDURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12631:SF10">
        <property type="entry name" value="BETA-XYLOSIDASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045748549" description="F5/8 type C domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1101" mass="118614" checksum="9189834528BFD9A0" modified="2025-10-08" version="1">MATTMCSQVWFPLLVFLSIGFINSRAQIVGSLTWNASVLLSSNYDATTALAIRDGNDTTMWQSGACFPTSYITRVEMNALYWRCNVTSACTASSGSSNLYGATDGSMTTSGASIAFSTANSYAWFRVSLPVILNITRVGIRGVFASTASNTLFVEFASGDRLAVLYNATSWTFISSTGSWTGAVSLLVTSSAAFTITEMAAQYGACYENATVDMGSVQTIGSIRTRYWPGSTGVASWLLASTDGVKYTDVVSELMGQAPLDPTRLSALTLTLASPLQARFLAVRHKVVETDWAKVYVWGIDAFPPAPPPPPPSPSPPSPSPPSPSPPSPDPSGIPWDNFAGTIRAYSYQGAVNVSSNPDPKALPHLVDGNDNTQWQSDACLPTGYTSRPNMNPLLYLCGGNSSIGAPASYCTASTGSTKLATATDTDVYTGAVIAVDPNRVAGGVIDGAAFFSAIVPGGPRVISRVTYKGFSRENVSVVLVLQPAGGAAQRVVVATLVPDINNYAWTGVPGPWEDVVEVRLESTVSFTLTEVAVMSAPCVEWAVVDMGQIREVGVLRFRHWSPDAEHTNMSVSADGVNWTIMKSFMKPDQLDTTELYLPSVIYIRYMKITHKVKEDANWRKVYVWGIDAYDVYGRWGAPPAPAPHPLTLRGMMGVNGIWGWGFQDFSNRLHRVGKGPDYYTPVASWGRNYHSVNWDVKLPTRDPKYDTMSKTGTDGQWWLDWDKEYVGWKAAGLKVDASFQFTWDVFGPATWGPNVTDTAYKLGFKFGSHFGPGRAPPNVSLDAVEFGNEPWTGFNASTYAAMLRGFARGVKDADPTFRLLPCALQAGNPFAEDGDNGNFIGARIPPDVAPLLDVLNLHVYSWFRPPELNGTMIGVHPEHPGSTMNAVNNMMSWRTFNMPGKPVWVTEWGWDAHLPGEVCDTTLCVSQHAQALYGIRGLLVLARKGVQVADWFFYANADEGTTVFTRSGLRGSNSTNFPRLPVFDAFAAFMQLVGNQSFLGVAAERTDLYAYLVGPVNASTSAATHLVAWQPVAAGDGPDEPVVAASLSLARSPVAAWRVSGAGAVSVPLSSIVQVANSIQSGRTWTLQVSGVPLVIQLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUG9</accession>
    <name>A0ABQ5RUG9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_003055</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF77">
        <property type="entry name" value="8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="221"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="135"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="135"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI60844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="615" mass="64916" checksum="8B544432B1FD162C" modified="2025-10-08" version="1">MDGFQLWDDWLNSSLAHLKDAQLLRILRPTVWCNSSSEAYIHRADLDAWLAGQPPIVRHYDQGAAVTMSAAVVPGDSDSLCTYVVRHYGDMDYCVAPTDLDTAAAQDASGTCCRRCGSSARGDEDRCVCCCSRSSNKQIDPSRKSSSCANSRDNPHDSSSGPAAHCRSDAGVKDAAISLETGPANWVSGNAGCTTSASSCSRSKESRNNTDPGLLVRLRLFSLNDYLGLASHPEVSEAAARAARQIGMGPRSSALVAGYTHSHRQLEEGLAQLKGTEDCLLFPTGFGANLAVMTSLAYAAPTSAATGGCSASHTDGGSSALAIFSDELNHASIIDGARLALRGSGASGGAVQLHVYRHNDLGHLEDLLAATPPGVRRLVVTDSLFSMDGDFADLQGLSSLRRRHPFLLAVDEAHATLVCGEGGSGAAEAMGVAHEVDLHIGTLSKAFGALGGFVACSHSFKQLLLNRGRAFVYSTSLPVPVVEAAGAALRVSQRESWRRTHVWSLVHRLGTGLGVPALSPVVPLVVGPEGPTLELSSRLLREGLHVPAIRPPTVPPGTCRLRVSLSAAHSYDDVDELIRLVQQSDVPLVRLPHLLDPRAALLPRCSGSLLLPSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV21</accession>
    <name>A0ABQ5RV21_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003780</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037">
        <property type="entry name" value="FORMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037:SF24">
        <property type="entry name" value="POLYCOMB PROTEIN PCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="414"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="224"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="306"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="390"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="701" mass="75042" checksum="3FE179B7B83DD47D" modified="2025-10-08" version="1">MGKREALCAPGAGDQVASPYIGVQLQGRWVLMCLFAAIAIPGGLAGPLAGTNFPFEPCIKTPGTSRLSVAFKNTSWDSRRRSTRVCLGFSVGPEEDCDAGALRCCQTGFNKFKFYPDPSCRRAITDIVYQTNSGRVIGVDSVYFENFGDTFIGKVTTLTFSLANANGGVLCWSLVDPCPTLLQFTHPATRDKNLFEVALYDKKVDNYECCPLGAIPTYGTDAPPSLPPAPPSPPRGPSRPSPMPRPRPPPPRSPPPPRRPPPSPKPSPPPRMPPPPRPSPPKPPSPPSPRQQSSPLVRLPPAGTRQPPPIRSEPSPRAPSPSPPPPSPPPPSPPPPSPPPPNPPPPNPPPPSPPPPSPLPAPPSAFGSPSPVALSAPRPLSPAFQPTPFLRNTQDAQQSNTRQGLIEPVVSYTQDSRTYGIFISPGVSFSDAAARCGRGGGFLATLLSVSEWSDLRSALGDSRLYMGPGDRLSVWIGLDPKAGSNAWLDGGAIRFWPQLSPPKQDGTCYTASCNLTETETPTCSWEPTPCSSVAVQGFVCKTYINVVSYINASPTQGKTYIYSQYGMYSSAAGNYCRRLGGYMASYNSQDEFNTVIFPLVTGEVEIPRSAMNINGRVVVWLGFGDPAATRWQDGSPVTFSRLNTTGPPWCYVLNCPYGIEKGGNPDACLWNLLQTCLRLSVFICELPGVHERGSGPVSLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVE7</accession>
    <name>A0ABQ5RVE7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003603</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34407">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34407:SF1">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045630685" description="SGNH hydrolase-type esterase domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="656"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="716"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="735" mass="77154" checksum="336A1E22FB4E8643" modified="2025-10-08" version="1">MGPIKDFLFILLAIAKLPLSICYQHATSPRIENLTDVEIKYANIYWYYDPAARVVHANKTYHRSGRSSASGPHISLEYRFVVPLPERLAGLAYVGHAARLRLVLERARNGQPLIVGALGGSITFGQSVGGWKGSYVKIFTDWLNAAMPPPHRQPRRRAAEEGNATAAEASAQSGSAATNALAGAPLAAAQRTLKGLSAIASVGMADVERGDTAAVKDLAAAGAIRGQAAGAEQGRVTALTSRKMLAGIAAATTGAMLDTPDATGGAKPPTEATAPQRDGGTGSYRDGHAGSWRGQPLRHDFLNGAVPGTQSAYMSSCLRYHLPPAVDIVFVEYAANDSPAPRWTFADPCRRSLERLLRKLLNLPSQPAVVLVNMYAIGAARGKYLHTAERDFMEFATYYSLPAVSLKAAVLPSAAVAGAAAVSLGAIFNGGHNHPGRGGHVVITELLITLCLSLLRSSTDSEGSDTWVAPVIDDAGAAPAVTAAAATDALAALEAVASRPLPPPLANGNYESSESTCYIEHDLQGLILAPLEGWEWTDEGRGKWGYVAMAPGKTLRLKVNTRLVGNRSADHAAASIIVQIAYLQSYIGMGTARLVCMEGCRCRPLTIDAYDKRDVSVTSMADLKVTQHPDCVLALTTKGPSQQAVIAAAGNWKNTTASGGGGDDGGSPAEGLSASREGESGAAWNKFKLMGVVVGEEPGAEEGGVTWMRDESHETAHAMQVTGKGSSATTHTHKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWH8</accession>
    <name>A0ABQ5RWH8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004458</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF95">
        <property type="entry name" value="CYTOCHROME B5 TYPE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0349">Heme</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="45"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="136" mass="15536" checksum="E1C0C65FCE28FB29" modified="2025-10-08" version="1">MDLTQTLIIAAVAVALVTFFILRQTRDYSKQLEQLDPKKKKPRQFGMYTLDQVAQHNKRDDAWIIVQHKETKEYRVYDITDYVDEHPGGESILRNIGGDATEGFHGPQHPITTYVLVEEYCIGKLADGEVPTSEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWV9</accession>
    <name>A0ABQ5RWV9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Reverse transcriptase RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004168</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35046:SF9">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35046">
        <property type="entry name" value="ZINC KNUCKLE (CCHC-TYPE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="193" mass="22206" checksum="20592D7AF821978F" modified="2025-10-08" version="1">MLAVVHCLRVWWHHLKGPQPVTIWTDNLPVSASKTQPRLNRRQVRWLAELEEFNYVIEHKPGKTNVVADAISRRPDLRVHTMTVSVAKAAIDLATTIRRDGPQDEIYTRLKEQAIKNSKEVPPFKLYKSLLYYEPRGGLRARLYIGGYLHHHLPLTPTVQTQAWPPLGKSQPLPIPERPWQSISLDLIPDLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX58</accession>
    <name>A0ABQ5RX58_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="236" mass="25485" checksum="872C7773FAEBCAE2" modified="2025-10-08" version="1">MSLRRSHGRSYGTRSLGQPGGASSTLSQITQMRVPRKQIESSKTSFPVPATPVPVGAGGTAQHTPGLGALELPKGTTKSGRNDEPPGTQDGRTSKRQKQPAHGEDALIMVLATNAARIHQKEEIQAARCRQAKSPQPSKARNSSRNATNSSKAAAKKVTNDGDVRPSTKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRRKKVKSASEDVRCTWARRQRGSSGSKKKQVTKCPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXN2</accession>
    <name>A0ABQ5RXN2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004613</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="13454" checksum="95A8289DB43BE048" modified="2025-10-08" version="1" fragment="single">MRCREYRTAVPNGQDPEKWKDGEEGQELFWRVLRVMDRPDSYWEEVEPLRVVMQSEQDEWEQRRQQQQQEQQEQELQEEQEQQEEQELQEEQELQEEQELQEEQQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXY6</accession>
    <name>A0ABQ5RXY6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="501" mass="53740" checksum="519F5446E36915DC" modified="2025-10-08" version="1" fragment="single">SQKLSTAHANRNVPAAAPSVSERPVPESSKDLPGSGMWLARGVRAADFRGAIRDQHASCNSQHDVIVDDRPSAKRFVIATNQRYAALFSASKGCRALLGNGSVAHHKAPATSTTEGSTSARNSDIYVGIDHDPVGVPCQPAPPAFPVLERLLHRRKRLSALLMARCTRAAPSLGSSQLRSDEHTRSAAPSAFVSLLCDTGCLACEQRSVPGRDHTMPGEDRARSAGSMLRPRFIATGFAVGKASARPSDGGDQRQAFDSRSRLRNTEMPGVSAAAVIRGHPAMATDAYTLMRAWRQQRAAAAAIICRAARRYLDAGAAAEKARVSAAADSLAACIKARLLAAWQGVYHRRSRLRRQYTRLVALSLKAKYVACVYPGPMAENMDLAEIHAGLVAELAAAVYAPDRTSRSRTTSNSVLDGTWDSAHGSCQHGDSSYGVPYGLVHADGWGAAAASHRRYCTAASADEGRYGLAVAHRRWWLLRAAWCSLIVHAIAPLRWRSLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY82</accession>
    <name>A0ABQ5RY82_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="129" mass="15076" checksum="9CA65D1176C27D34" modified="2025-10-08" version="1" fragment="single">KTLVFAVGFNKKRYHNNNDIVRSYIRPTYARVRWQQWAFFKSSSSPCNGGTCRGKRLMEHRRGCERYDDAVAYTAWKLQKHIKKDFGYKIWAVYNDCTTLYVTYWKLCYLFSTLTRLAEALAGSSSCGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYZ2</accession>
    <name>A0ABQ5RYZ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_005283</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00317">
        <property type="entry name" value="cyclophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="22"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="182" mass="20002" checksum="2916A257BCCC6AC1" modified="2025-10-08" version="1">MEFESADAMLEFCRSTGRTYVAFSLIQTGRELGTVVLELFTDIAPATCANFLRLVRGGQHSYADTPIHRVVKSGWIQGGDVVDGSGKGDPGFTLPDETFAVKNNDKGILGMCNKGQPHTASSQFYISLSPLPFLDGKRVAFGKVLTLDGLECLEKLENLQTYINERPVPDVTTTKWHVLYEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZS4</accession>
    <name>A0ABQ5RZS4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_005334</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18024">
        <property type="entry name" value="DEXHc_Mtr4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1500.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025696">
        <property type="entry name" value="Beta-barrel_MTR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF25">
        <property type="entry name" value="DEXH-BOX ATP-DEPENDENT RNA HELICASE DEXH9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13234">
        <property type="entry name" value="MTR4_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="100"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="334"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="631"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="693"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="642"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="662"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1099" mass="118370" checksum="A2F5191320D5EB78" modified="2025-10-08" version="1">MASGTALGKRPAVASGYVTNPGDGDGMTAPPPAKAARLSDERVARLPVGANLIEVDGKSCTHEVAWPPDQEGSRLPPPAREGPPARVYPFKIDPFQQVAVNCLEAGHSVLVAAHTSAGKTVVAEYAFAMALRDKTRVVYTSPLKALSNQKYRELAEEFVDVGLMTGDVTINPNASCLVMTTEILRSMMYRGSEVVREVQLIVYDEIHYLRDKERGVVWEESIILAPREARFVFLSATIPNAREFADWVAKTHRSPCHVVYTDYRPTPLQHYLFPAGGDGLYMVVDERGVFRDDNFQKAVGVLTETDAAGGKAKGGKNGAGGVNPVGGSESQKSDIFKLVRMIMERNYDPVIVFSFSKRECEALAAQMAPLELNSDDEKALVANIYWSAMECLSQDDQRLPQITAMLPMLQRGVGVHHSGLLPIVKEVVEILFQEGLLKCLFATETFSTGLNMPAKTVVFTNVKKYDGGVFRWITSGEYIQMSGRAGRRGLDDRGVVVMMLDTRLEPPVAKEMIKGAPDTLYSAFHLGYNMLLGLMRVEGAEPEQLMAASFRQFQVERSLPSLEARVAALEAQRDAIVIEDEQSVQQYYALLDQLLSLRASLRVALNSPRVCLPFLQPGRLIRLLPEPPGLGKPLPDFNEPPAAGGSLSGTSTEATTSSGYGSNNGGGGGGEAVLSEVTHGVWGTVVNFERVSGKNDGAADDGGGGKKPKKGVSSSSCAGDFIVDVLVNTLPPPGGAAGAGGSSGTLPRGAHVRLLPPGEKSGVPLVVTFSLAQVDRLSSVRIYLQRDLRPLDARRAGRGSLAEALVRLGSQSKSGRVPLLDPEEDMKVGDKAVRKMQSKLESVESLLAAHPLAALSAPDLRARLGALQRRNAAEEAVAAARREVKAANSLILKDELKARLRVLRRLSYMDGEGVVTVKGRLAAGLSCGDELVLCELVFAGVFNTMSLEALAAACSCFVFQEKGADGGGPKLRDELVGALAAVKDAARRVAKVELDCKMALECGPVGGSSTNLEDYVGKFRPDLMEPVAAWVRGVKFAELAKMTTVFEGSLVRAIRRLEELMRQLAEALKGIGEVALAERFEQARERIKRDIIFAASLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S000</accession>
    <name>A0ABQ5S000_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peroxisomal biogenesis factor 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_005906</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxin-11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF50">
        <property type="entry name" value="PEROXIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="25542" checksum="E1C05F56F7119B75" modified="2025-10-08" version="1">MAPTYADHLTVVKGFCDKADGKDKLTALIQYACMFISAGEPGNIKKIQASVTAARKVFRVMRPLELVTPLLIQLGYTGKQPVLLEAINKVKAILMAIYFGADHVVWAHQIGLISDKKIGERYQKLSLWSWALGSVCTIAAEGWQITAADVARKEGETEEEYGKRMEEVKKQINHRLFILIHALFQAALAAGLLQVAPLKPRTVGFLGVVASAMNCYMLLPAYPKMAAKPKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0H9</accession>
    <name>A0ABQ5S0H9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005825</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="1">
        <location>
            <begin position="234"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="34088" checksum="FD2B6610328986E7" modified="2025-10-08" version="1" fragment="single">QFTSKFWEALCKLVGMRVNLSTAYHPQTDGQTERTNRTLGDMLRNYAGRTPLVWDTYLTAAEFALNNAVNRSTGKSPFFLNYGFHPALPVWRELEVNVPAAKLFAKSFMSRITDAKSCLDAAQQRTTEYYNKSKQDVTFSEGQLVLLSTKNLRSLADGSRKLLPRWIGPYPVVRMVGNVAVQLALPADMTIHPTFHVSLVRPYRGTEPVPEPANTPAAIEPVPETWIVGKQKFYDVERVLDYRARRVGQHQRKRTVHEYLVKWTGYTDEHNSWEPARNFSQDMKPLLEEARKRATQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Z7</accession>
    <name>A0ABQ5S0Z7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Photosystem II 10 kDa polypeptide, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_005996</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the psbR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006814">
        <property type="entry name" value="PSII_PsbR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34369">
        <property type="entry name" value="PHOTOSYSTEM II 10 KDA POLYPEPTIDE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34369:SF7">
        <property type="entry name" value="PHOTOSYSTEM II 10 KDA POLYPEPTIDE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04725">
        <property type="entry name" value="PsbR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="9" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15123" checksum="5767E157ABC9BF48" modified="2025-10-08" version="1">MATMQLAPRGLAPLRPRVSSRIVVKPVASGGGKTDITKVGLNSIEDEVVKMNLMGKSRFMNNKEWKDASGRKGKGFGVYRYADKYGANVDGYSPIYTPDLWTESGDSYKLGTKGLIAWAGLVLVLLAVGVNLIISTSQLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S117</accession>
    <name>A0ABQ5S117_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005772">
        <property type="entry name" value="ATPase_V1-cplx_fsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01101">
        <property type="entry name" value="V_ATP_synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861:SF2">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015945">
        <property type="entry name" value="ATPase_V1_F_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="123" mass="13391" checksum="96D88B1858E89B24" modified="2026-01-28" version="1">MAKNANITSADGMLLAVLADEDTITGFLLAGVGNVDLRKKKNYLVVDSKTSVRTIEQAFKEFSAREDIAVILISQQVASQIRHVIAAHNKPIPAVLEIPSKDCPYDPSQDSLLKRVKLIAGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S122</accession>
    <name>A0ABQ5S122_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="319" mass="35924" checksum="3D6F0931BA5FADA9" modified="2025-10-08" version="1" fragment="single">MQYYCAAKFTDAWLQKHKFSRCQDPLGQEVYACTQCPFTGRTRSQLYGHMKRCRLSFPEDTNLNMDNENDFNTSDDHNEDHSSSSKDSSGHTSEVLSGDSSGHSQVQEETALPSSLVSALIHVMDDLDPANPNHRQTLEYISAILDNLCQHSYPESEDDEPDAAELGVLSQLGAMDHGQLQLLEPTNPAELLASGSTVQAYKERLDDPLWRVTVNNGDGVVVCQEARITVGQFGYAWLKEKMAGRVRDVVADRQLRFLRDICLPPGNHVPPSLYVLRKMLGIPDPRDFEQHICLSEKCRFPPLPTKDWMDHRDDTCNCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S144</accession>
    <name>A0ABQ5S144_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05467">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="2">
        <location>
            <begin position="38"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="738" mass="77461" checksum="5A9C1C0DBBB5ABBB" modified="2025-10-08" version="1">MRPSHPLWRRPTPQQGRCRCLPRRAMAAVNISQRDVQDYRPRLVNVSFKVEYKCSYGQSLTLVGDSAPLGNWSSKRGQRMHWSRGDVWTCNVMLPAGSDIECKYVIVDDQGRAQRWQEGSNMVVTIPDTYQGLPPQRYDVGVSWCKNHVNYRCVAAPPTNAAQAAAFFMEKLKNTCATVVSTAAAAPAAEAAPPMMAWALSKGASTTAGFNPTPLVQHVAEAHTSIFRLPSASAVQCAAIDVPALPAHQASSTPSLRKQVAANESLSQDTGNECKVATLVQAAANSVCEAHTAAGSRLYESIGAAANSIGLLSSFQLSSASSEGEPTMSITLPPRSKSPPVKISLSSSFSKLLDMPALTRNGGSNASTTGTGAVISTAIMGKTTASSIQSHAIDPVVASSSHTAHIGGGAPKPQGATNSTDKPVVSPPYATANTVNRSIAASVSPTWGMEYDIYATAYGLSYMHTSFDLESYALYDQTGPAPSSSSLPSMSPGRLYHESAHGFLSANQSCGADPLESAVQAALSSSPDTKTAREASFFPYTLLGATDANTSLALGAAAGSSLADHVSDVATPAVVSANAAASHPPDAVASAPVEVYRQVSRSSTSISDWAGRTLIEMNCGHSLIDEDSDEDETADDEAAVAQALATPAAPADMAALLTQLGTALGRSVRLRYDGIDATAQDLLELDRKIALAASKLYRQRDTLLTGWLRKETRRQLATVAATSSIRPPMPASNTVTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S176</accession>
    <name>A0ABQ5S176_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="312" mass="35274" checksum="70AD5083F2AADDA5" modified="2025-10-08" version="1">MEIARMSLPTIHGGLGPRTQPLGCSKLEAGPLPSSLPLFSAFHAYIIRQSVNTRSGHGDRRRQRSLRQRCSSRFLAEKLEYGGYCTCGEKHFLRATPAALVAARKLIERLDTEGRIDFDSLTPDPLLHIDYVWTKGPGRMFGVMVCIKPDGHGDNVDGAGMVHNEPIVLKAFSGQMSNYWHVPGWVGPVATIVNTTTLYHNYRTLTEAYSARLASLNLALQYHHHVRGQQREQQAPRRGSELSAGEAFLKRQLELLRARRKALSHELLERIQDSYRLCNFAGWNLRLLDVYAEAVHRQQRGQQEPKDEQDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1A9</accession>
    <name>A0ABQ5S1A9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_006698</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051836">
        <property type="entry name" value="Kremen_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269">
        <property type="entry name" value="KREMEN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269:SF16">
        <property type="entry name" value="PROTEIN SLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22633">
        <property type="entry name" value="F5_F8_type_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00607">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046699189" description="WSC domain-containing protein" evidence="11">
        <location>
            <begin position="33"/>
            <end position="1713"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="12">
        <location>
            <begin position="45"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="13">
        <location>
            <begin position="1491"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="13">
        <location>
            <begin position="1613"/>
            <end position="1708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="419"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1586"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="421"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1586"/>
            <end position="1612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI63661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1713" mass="172867" checksum="3BF762F6D1A3F2CD" modified="2025-10-08" version="1">MRRRTSPHTQHLLLVLHIAVFAVLLQNASVDAERRTLLQTSSWASPPPPPRVVTTNIAAGKPAFSSSVYLKNETFGPSRAVDGLIDRSSLFISQPGDANPWLSVDLGETFTVTRVIIYLRQDCCGNETIRAEVRVGGAQVPNANQITYNDVAYKMIGSGTTGAVLNITVNPALTGRFVTVQSFNGPANLSRLGVSELQVFGYPAVCSMLQYGAGYGTDALALGTFFLADEGECCQACYISSGCLYWDFEILSRTCRLKTAEGILNAPGQNYPGFFLDPNRVAGVKRGIATFKRHNGLVFTPDNVTKWIWSRPDSLFNPPPSYITYYFARTIYLPEAVANVMARVVVDDNVTVIINGKSRLPNAFWPNITNVTVDFVAGYNLVLLRCYNRQSFAAGAAAFWSPNGTILLRTDEKWAFVEAVPPLPPSRPPSPSPPSPPRPPSPAPPRSPAPPPPPPPPPPPPPPPFPPSPPPPTPPSPGPPPPPPPPPPPPSPPPPGPPPPPPPPPPPTPPPPPPDIPPPPPPPPPPPPPPPPPMPPPPPPPLPPPPPPPPPTPPPPPPPPPPAPPPPPPPPPPAPPPPPPPPPPTPPPPPPPPPPLPPPPPPPPPSPLPPPPPPPPPPPPSPSPPPPPPSPLPPPPPPPPPPPPPPPPSPPLPPPSSPPPPPPPPPPPPPLPPPPPSPPPPPLPPSPPPPPPPPSPPPPPSPPPPSPPLPPPPSPPPPPPPPPPPPPLPPPPPSPPPPPPPPSPPPPPPPPSPPPPPPPPPPSPPLPPPPSPPPPPPPPPPPPPLPPPPPSPPPPPLPPSPPPPPPPPSPPPPPSPPPPSPPPPPPPSPPPPPPPPPPPPPLPPPPPSPPPPPPPPSPPPPPPPPSPPPPPPPPPPSPPLPPPPSPPPPPPPPPPPPPLPPPPPSPPPPPLPPSPPPPPPPPSPPPPPSPPPPSPPLPPPPSPPPPPPPPPPPPPLPPPPPSPPPPPPPPSPPPPPPPPSPPPPPPPPPPSPPPPPSPPPPSPPAPSPPPPPPPPPPPPSPPPPLPPPSPPPPPPPPSPPPPPPPPPPSPPLPPPPSPPPPPPPPPPPPPLPPPPPSPPPPPPPPSPPPPPPPPPPLPPPPPSPPPPPPPPSPPPPPPPPSPPSPPPLPSPPPPSPTPPSPPPPPGPPPPSPPSPPPPPPPSPPPPPPPPPPPPPPPSPPPPPPPPPPPSPLPPSPPPPPPPPPPGPPPPSPPSPPPPPSPTPPPSPPPSPPPPPSPPPPSPPSSPPLPPPPSPPPPPPPPPPPPPLPPPPPSPPPPPPPPPSPPPPLPPPPPPPPPPPPSPPPPPPPPSPPLLPPPSPPPPPPPPPPPPPLPPPPPSPPPPPPPPSPPPPPPPPSPPPPSPTPPSPPPPPGPPPPSPPSPPPPPPPSPPPPPPPPPPPPPPPSPPPPPPLPPPPPLPPPPPPPPSPLPPSPTPPSPPPPPGPPPPSPPSPPPPPPPPPPPPPSPPPPPPPVPPSPPPPRPPTPPPPRLKPDCYVGCYVDSLDKRALPVLLWTSNNMTVNFCRELAHAAGYNFYGLEAGRQCFAGDDLGTAIALGNSTNCVWPCGGNPHQVCGGDFAVVVYKSSFPPPGSPPPPPPPPKPSPPPSPPPPGLPPGDLVGCFADQLLNRSLPMLLEVSPSNMTVDRCRELARAGGFNYFGVESGVECYAGNTLDAAWANGPKPGGCTYACAGDDCQICGGDWAISIFTTELPQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1U6</accession>
    <name>A0ABQ5S1U6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VLRF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006927</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="1">The VLRF1 domain mediates binding to the 60S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANKZF1/VMS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041175">
        <property type="entry name" value="VLRF1/Vms1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18826">
        <property type="entry name" value="bVLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52044">
        <property type="entry name" value="VLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VLRF1" evidence="3">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS52044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="144" mass="15901" checksum="47C090E81699A35F" modified="2025-10-08" version="1" fragment="single">SAALGVWLGGRLVRHKVLTGYTVRHSAGGSQARRDRRTRGGGGRSVGASLHRAEAVRLWQATAARLAEWVSELSYCDMLIRCGDARVFAHVYETRRPPAPIGPRDPRWEGAGMSVPRPRLRDLFAVYGRLSTGRVQNLLSTIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S236</accession>
    <name>A0ABQ5S236_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046259714" description="Protein kinase domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="817"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="751"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="806"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="647"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="751"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1108" mass="115939" checksum="218134A24902974F" modified="2025-10-08" version="1">MAASALFFSLLGLGLRTAWTYETLPLLKSVICQDSADLVSALLNQSVGMAQLVNDLMLKDSDWPASTILVGRNFTVEGVAARPVVIDMAFVKWKVQFFNGAFATLRRLVLLRFRSGSYNQAPGLNLFKPLPPGDSASLILDQTFLIMPICFPLAITRQTVLDQVDNRPSVFPGKDVYILPDVLPANCSNSTSSYILDRCYVYAGRYMDVAFYGMEVAQSSGLPVRSGYLLRATDTQMLCIQIAPEECIRSLGPVGCILFLGSNSSSPLAPLPAPGAARLYNDSAGAAAALAAAATTGGSTGPSKQAVVLAAVLCPALGGGLMVTLLVWVTVKLIRVSNGNDNGKGNDCDAVGGGIGGGRNCALACVCVPARNGSTSNTIKRRAAASDESRSSSVLGTQPSAAADDDVAAAAATAGGKAGTDGTAAGAGTVSVGLFPVTDRPACVDPHCHPAASRPAAGGAALVVVSPLTQPRADLKTNVELKVTRTAPAASSTSPITLACPPLLDAPRNGAHQGDGDLSRRRLQDEDAGARGPADGRSNGSGGGGGEGASEPPGPIEGENVVTLLLGVLGKGTFGRVHVGMYRGERVAVKVVELGFEVEAPDNKANVVGHGRVEKKGEECQEPEQQVTRKVMNQAAGTHRQLEEQQQRPSSSPSPQQQQQQQQQQQPKLYQQQQPHYPYRSCENNPATVLGSCGAGSSQGNDDSGRNDQVGDRPGPPTRGRGDLVGSNSSPVSIASSAALASFMTGLETSSSTNAASAGAGASGNTAKQQQGSGVSFTICPSIIPAVTPVCAPSVPEAAVADRRPLGGTAQLHPPSAEPITTLGGPDAADIDQASAQPALVAPAIDDQSVGLGNHALRQLVQEVQVLGRCQHPCIVRLLAACMTPPRVCLVLELMDTSLEKLLFGRRRGAAGNGVGEVGGDAAGNHGTRAILSLDKVLHIALRVAQGLEYLHPSIIHRDLKPGNVLLKDPDSPRPIVKIADFGLARLRVITMPTMYPCVGTVPYMAPECFDIGLNVLTHRVDIYSFGVLLWTMLTGQQPWQGCPAIAIAYGVGILNRRPPLESLDDERCPKRLRHLMVRCWDPDPLRRPAAAEIVKEMLIIQMQRPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2L2</accession>
    <name>A0ABQ5S2L2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006717</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006189">
        <property type="entry name" value="CHASE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50839">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="CHASE" evidence="10">
        <location>
            <begin position="124"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="459"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="533"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="919"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1047"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="539"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1047"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1061"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1070" mass="111154" checksum="C18A945D4903B142" modified="2025-10-08" version="1">MVEVVHLRPLVLVPGLMVLCIVLGLGLWALIAASNAEERFRHDSAQKIAIDKAIYIQTELDKTFMPAYVVATTIQQNPYYYPPQPNSTNITNKWYLENKNFDKLAGDLIALTKAGSVRTVSAIPHGIIRTMYPLYGPEDKNWRAIGKNWLNDSENQAPVKRSLTSRNLTIIGPYYLAQGGIGIVGMQAIFVNASEDDTFGIPPGPNGIIGPISDIAGMLDLTWQPQNNAAPQFWGLVTVLLSWDALRDNVTNLRDLSAQGYDYVLTRPDKDRNLAVDWSPGIDPARPPGFNSTALGSRPLIYYDLAQVLPDPVIVKVPLPNVEWTLYVSRTGGWVPSWKAPLIAMVVIISLILSLLVFVVLVSRVQQRRLLRDVVEAAGKLASTTRTLEEEKNRMQALLARHFDLIDLLEGGMAGRDGYKSGGGGGSGEGDARIDILRQKMLLTGTSLRREQLGEAEQVTIQEMLGEGTFGKVYKGLWRGTEVAIKTIVLPAKMSGKEKREKMAVMEAAISSSLAHPNVVQTYTYHIRPLRDSSAAPSVAPPSPSPSPSPSPLGGGAAGAHGSDTSPNAGVIGPGGTAATNVDGVGRTQILADAGAIVVGSPDSSVSTASISINANNSGAATAVAVAAVAGIGGGGGGGGGTGTEHADTIPAPTAGTAGSQGVGPRGVSLDQPATAMGGIHSYEVQLVLEFCDRGCLRDALDGGAFFSAEGLNYPAILETAADIAKAMLHLHMNDVLHSDLKATNVMLKSTGSDSGRGVTAKVADFGLSVRLDHTATHISHAFQGSLTHMAPEVMLKGHISKAADVYAFGITMWEIFTGGQPYRGTPGALLGHQTSKEGRRPTFPLGTPAAFKELAERCWHADASVRPTFSEVLDLLTQMRAEIPGPTPPLQSFAPLHKKMALAAQQQQHPHPEPGGIGQFGGQQGDGMQGVGGNCNGGGGSTTGSGVGSGIGMLHKIGGPAAIVVGGRLVSLNAAMHSADSGVSGGRMLSGNSAAAGGTLAGLGLPGVLETISEEGHGDASAKLAPPPTVAVVAAAAAAAAVASAAAGVGSGEPPATAPGDDENEGMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S332</accession>
    <name>A0ABQ5S332_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_006929</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="5">
        <location>
            <begin position="1220"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1675"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1757"/>
            <end position="1788"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1799"/>
            <end position="1827"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1882"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2006"/>
            <end position="2038"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2114"/>
            <end position="2145"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2146"/>
            <end position="2188"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2189"/>
            <end position="2220"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2234"/>
            <end position="2276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="41"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="98"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="355"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="608"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="713"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="823"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1173"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1924"/>
            <end position="1944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="393"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="493"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="608"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="647"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="760"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="787"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="837"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1195"/>
            <end position="1206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI63829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2432" mass="245456" checksum="4815E85A94C57B4E" modified="2025-10-08" version="1">MEQRRPGELALWACARLRSLTGAKRRHVAARRAASVAMQSDPWVSADQRNASSMTSRPRVGGASRPVTLRPNTQWFNPDEFEESLYAPQNDVPFGRMGGAGGGGARGDGGLAGGGGGHAGRGGFVSSLSGAGRGAGAAGRGRGGPTGGRTGPLVGGSRYETLGSADVHGSNYLLGGGGGSDADIRGRGNMNGIVGGGGGNAVDILARSSLGGSVSGGADILGHTSGGGGVGSIGGSGVLPLGRAPSFGRLATLPPALKLSSSGEVLELEPVDRQGAGGGAVLRRVGSGLLAGGPLPGPVGPLGALKGPPPPSQIPDRPGSITTTMTQTDGEGRTIVTKTVVTRTHSGRIRTEVTTTTSGPAHLINGGGGGGEGGGALGPLDEPPPMSPGVRLAQGSGLRAARSGGSAQTSPDAVKAAASQVGRLSGMTPAAAASSPVRGAASSGSGTDGAALQSPRRPSITDGLGGGQVSVPPPPPGRRPSQTGMLAGPPVRPVSGGRSASVSAAAPGQLHAPGTGPSANLRSTWGGATAAGGGMNAAPVAAGSAVAAARMTSPARLPVGVKPAAAGGGGVAGAAAAATAGSGRAPSAGRLRMSPAFAAAHSLGSGAAAGGGGGGIGSNGGDGSKSAGQEEVFMFGSGGGGMGLAQRPSSGRYGNTAAGQLSPPSSPEPDTWSPAAATRLQPLSGGGGRSAAAAAAEMYGATPYHLHDEDATLSHLHDDPTGPHPQALLRTSVASPVPGPRPSRAVRSPSTAGLTPMAPPEALAAAARRGPVPSPPAAKPAGTMARPAPPVAAPPPSSPPVGLSPKAAATVTAAAAAAAAVGGVSGGGGGDTTPVRTPQPPASPKPPPSRSSDGGDESASRIRQSIGKAVFAAKMMGARRNAGQCAVSLAFLRRFRARFVKERTDAATLSTSQVVQEVIKPMTASSACRLTDLNMNDLVSEADIGPPDYFISHAWSMPFLQLLDCFFNHLQGALDSTRVWLDIFAVNQHPGAEQKDDLENLRTAIALSQATLVCLDARGTPLERVWCLYEFDNTLALKGADALVLLTPGFSTREIASIFRSIDVDAAKATVLSDKHMILDDIRAKHGSTEAFNTQLKLRFLLDPLDAKPDMMALAARAGTDAAQWNLRPVAEWLASPQGPQVAVLTAAPGTGKSTITAVLCLGPEAAAEVAAAAAAGRKPERPSTAATGGRRRSSTPGMSGRRISTTGGGAGGGGVTVHAFHFCKYSDARRQDPVRVVKTLAYQLAQSLPELGRYFCGLDPGTLMQLNQPDTACRELLVMPLQQTATVRGQQILLVLDALDEADKNPQPSTGGAAAAAAAAAPSAPSALDNKVLQLLLHQLSLLPDNVRILTTIRPDPHLLGPIRDRFPNLRELTPGMLRRQDKTHSVMQTRLVERFGDACAKQLLAAGGGGGGGGGGGGEASLVYYPVVALLRAPLERVPASLPEAFGAVFTQTWPKDPALATKVKQLLEVLVAAQEPPPMSLLATLDVSGALESLPGWGVLFYERDHAVHVLHRTLIDWLEDRAAAGPHYADARRGHALLGKHLLTSRPLGVYGARYLVTHLIKAGSDPALLDAALQDLAYLEAACRTGDVFKLHAELASLPDERTTDVVADVVRWLGLNGAVLWNHPQSVTQVAAEAPRCSSVWQVGDRSAKKNAVDMLNEPYFWPLEVMALTQHKAAVPDVTYSPDGRLMVSASLDGSIRLWDAHTGEPKAALAAPPALCLSYSPGGELMATGGDGGALCLWDPSTGLQRASLTAHKDAVTAIAFGPDGALLASGGRDKMIRLWLTINCSKVADLKGHTGPLLALAFSPDGAVLASAAHDDAVPRLWHVTEGRLQAELGGHRQGVLSLAFSPAGGLLATAADNVRLWDAASGAARGALTDHIFPVAAVAFTPDGATLVSADTSHTLRLWGVGGAASGCGNAGGRPKGRGPPGGGPGGGPPVGVVPLAVLEAESGGQLSALAVSPDGGFVATASATGELHVFAVQRAAAAAAAGGAGGVFTVRAAHAGQVRRLAFSPDSHSLVSASDDCTLRLWDPRVALAVDGEGGGGGGGATQAGAVTSSVHYSRSSSLTPLLAASLGEDGTLSLWDGSSGDMVSAPQRVTGAPRPVVVRLSPDEETLAVGEPDGSIRLWDLTKGTLKARLKGHTDAVTDLAFCSLNPALLASSSEDCRVRLWNTGNGRQLSLLTGHTASVTSVALSVDGAVAASGDESGVIRIWAVGDGGNGRAAAVLKGHSGAVFRLAFCPADGGLLASGGADCSVRLWDVGGGKQLAVLSGHKEMISRLVWGTAGRVLASGSRGAGSVGSVYVWDCRTGEAVYSLHAAYMAPEDGGRPGGAGGRGLCTVVSALEHAPHLTLVYETDGGSGGGAASPAEMEAGNGLLRLRQVPLYTSFAAPSCIAVAEGRFAMAFGSHVYFYRLHETRAGVGELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S334</accession>
    <name>A0ABQ5S334_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Photosystem I reaction center subunit IV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007547</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stabilizes the interaction between PsaC and the PSI core, assists the docking of the ferredoxin to PSI and interacts with ferredoxin-NADP oxidoreductase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PsaE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008990">
        <property type="entry name" value="Elect_transpt_acc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003375">
        <property type="entry name" value="PSI_PsaE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34549">
        <property type="entry name" value="PHOTOSYSTEM I REACTION CENTER SUBUNIT IV A, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34549:SF2">
        <property type="entry name" value="PHOTOSYSTEM I SUBUNIT IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02427">
        <property type="entry name" value="PSI_PsaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50090">
        <property type="entry name" value="Electron transport accessory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="5" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13455" checksum="C1AF88A2CCEC7653" modified="2025-10-08" version="1">MCCFKPRHRETSLLSLTTISEMQALSSRVNLAAKPQRASRLVVRAEQAAAAPKAAPKKEVGPKRGSLVKILRPESYWFNQVGKVVTVDQGAVRYPVLVRFENLNYAGVNTNSYALDEVAPVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S379</accession>
    <name>A0ABQ5S379_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057498">
        <property type="entry name" value="Rtel1_ARCH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23109">
        <property type="entry name" value="ARCH_RTEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent helicase C-terminal" evidence="1">
        <location>
            <begin position="215"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="529" mass="55286" checksum="DFA995B9821C8685" modified="2025-10-08" version="1" fragment="single">MTRPGSFLFEFLGQLGITAHSLSALNAAIDAAADILAAADVEAGRTSTKTSTMALQHLQSCLNLAFSTLEPLTNKQGAPPAHKGFRVHVHLQRSWGADKLPAPTLSYWCFIPGLAFRRLAALRLRSFILTSGTLSPMDSFAAELQIPFQVKLENPHVIAANQVWVGVLPTGPSGVALNSTYASRDSTAYKDELGNALVNVARLVPDGLLVFFASYAAMDSAINHWKQNGGAGGVGSTWERIVRHKAPVVEPRESSAFGAAIEEFRAKLDDPATQGAVFFAVCRGKVSEGLDFSDRAGRAVVITGIPYAVKNDPKVRLKRDVLDEEARAICSGISGLAGEGVAPLTGEAWYTNTAMRAVNQAMGRVIRHRWDYGAILLADDRFRTPATQRNMSRWVREHVVVYEHFGKASASLTKFFKDKANFQVLPRTLPGRSTNFSAFETVGVTNGHSSSSSGAAAAAAVAAAPGSGFGGSAAGQSLLDSVPAAIDVSGIGALAATGLGGIGSRKPTATAILPTAAGGNGSGSGGAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3D0</accession>
    <name>A0ABQ5S3D0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="509" mass="55881" checksum="875A96CAD00DBA04" modified="2025-10-08" version="1">MIDLKALKEQLKLLGHDLPDEQVVAILKEMNIDFNDKPPERNDAKESPGNPTRPKGDGKDTQSLPRAGSILDSFQGGQPSQQLPFSYTQNISRQSFNYGYTSPGHDPAIGLSHARLPSSSAQLRSCTSEGKNHGPGEGFKPQAASSDHSDDCYDHDSDEVDTDDKTSASRRYCAIEPFQPTLATNDSSCIGLNRSGAAFTPGQSARCSTKGPYSSGLAHLVCGPSDIAGRLSNVTLNEPVVGSIVRQSRLQQVEGKLRSKDMETTRPGVAARSQRPGSTITAKDQHRKTHGLRNAMSYDRDTQGPLPHGRGVKEEDVEYDAEVDVVVSGPDREPLMHRAPSEDEEWMQPGGPGASGRNPQDPYAAWLSGRVGPMANRLADALLAHGQLSPQASNRSFASHATSRCSIGSKRGAKKVDRVQRYQELQQEWSQNRFLKQASGTNRGTSRKPVNFHSHFASLHAAEEAERQRMLRETRARTKKELGAATEAPTSNRRDELRWQTRMRLREQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S552</accession>
    <name>A0ABQ5S552_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycerophosphocholine acyltransferase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_008124</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021261">
        <property type="entry name" value="GPCAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201">
        <property type="entry name" value="OS01G0585100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10998">
        <property type="entry name" value="DUF2838"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI64826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="547" mass="59896" checksum="FC388C105AE682B7" modified="2025-10-08" version="1">MRSEWSFQMQGASMSCPVTVMSHGCLVSTLDMIWLRRKALEPDPYGKCKHSSLEKMHMKMVFDTVFIPLQPNLAELQRHGRVLNGMNRKTVKIHAKSSTNEDSFLNEHLNYVQLGKVLARTASETKTGCDPVGFTGGVIELCILVGVISYSPTLCPLVYLGFAAVCLPYRIVSFSSRKWAFFLVDFCYFANLACCAFLVLRPEDRQLEAAIYALCEGPLAAALIAWQCAWLLGSPDHVISVLIHLMPGLAMYCHRYLAFSRQPLLLAARIALALLGKADLWAATYNGTNANGDTNSIHITSRHAAISWTQRLKAAAAVSSGQIPCPAFPGGVGLDISMHTAAHGPSRLPAAFSGDTQESPFAQMSANTRHMLLDGASSLGSSVTCYATPAGAPPTCPAPAWLWLGAAPLAFYLAWQILYFLIVQVAFRRMILSGGYDTSYRALARRAQRANSPLNRLVRRGGVARRLFMYGLLQLAFTLVTQLLAVVTYHSFAAATIWQVVKFLVPLYLGACHQYQRLPAQQLRAAMGVWRQLVSDGQTSSKAPAIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S582</accession>
    <name>A0ABQ5S582_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_008483</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF169">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SBP-type" evidence="6">
        <location>
            <begin position="51"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="125"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="266"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="353"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="778"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="875"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1669"/>
            <end position="1692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1705"/>
            <end position="1745"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="736"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="778"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1718"/>
            <end position="1730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI65057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1905" mass="199728" checksum="FDB5DED372ECCA03" modified="2025-10-08" version="1">MSGSSDSQPASHAWDASGTLWNPITMSSHALGPADGITPKLQRPRGRSGGRTTCQVPGCAESLVDCKPYFKRHLVCQTHAVAPIVMMDGVEMRFCQQCAKFERLAAFDAGNRSCRLRLFRRNVAKHRSQVAKRMAQSGDEPEARPARQHSKHARVPSLPDLAGNIGGDAAGGMVDVDGTGQPGTSSSQPQERSGGSSGEPDTRCWDAGPAPSHFRGALVCEDWRRQVASGPMKRTKVALSDAAAVATTEIEGSRADMDDLAMALVRNTHSKQRKRRQRTVPPRPRDESNSESRTLQSHNNSQDVNDDVDATLAPLTLGLRLDRCSEDTEPSANKAGVAGATTPELPLCTSALQSVSSNTQPPPKQLQRAPQQQQQQQQKSPSLAWLKERGPARPSRVLNGIHRGGAALYDTISELVTEPAQRSDAHAITTTCEAAARRLRDLAAGVSGFGAPPGTESTFFDDSTPSIMPLAMQALSNRPDLTHSDRQTLESLLGLAPGPFMALRAMSTSTSTSLDEQLYDIMHDVMKEGGSDTGWFSDALPAEAIPVAPPSEVGRPPVSNATGALAAACTYLGTPVTEMEKRGVLAAADGGGASRRELPYNQGHPSTGYGADDARHHVWGVNPGGLGATLANPALSEMGKGGSLHSDGTLQGAGRTPASTEVAAVAQEGPMPGWAHIPQYQPQAQQQFVWAAPQQHHQPQQQYHQQYQQQYQQKRTVLHQQHWPSQQQQPVQLPRQTSTELQLQELQQQQRTLEQKLQELQQRKMQLQHQQRVMAPTSLTAPASSATPCDSAPCAGGGSRPVAAGPVGAFPDQPNPQSHQQVYEHRHRNMQHHQQQQQQHYHHPHRPGADNGKAMPTIPFSVQPHGHPHPCPRLHSHPQPHVSAPAPAFVGQPGAMPPEASPDLKQQQPRPPTVIVAPPPPSPPGAVVMGAPPGPGSWMAPSWGNIPPQVRGGGGGNGDLPCAGHGDANAHTSGATANVLAGGWGACQATESAWDDYYHSPYDTVRLSVKMMECSPYDMDPALTQDMLHQMASEGGRCNMPPPLASVRPGCVRVVFNATVRAADCKRVADSRGVQGRDCDQEPFSALSGGVMAAVRQLAMTNDTAVVLQAGNNQVVSVDKMGREVTPDSGVAVAALPLPPPVLTRASMAAVYIGARGSFQVHGSGLSGPNVEFWGRIQGVHYQLQAQHRCGNSYALIQLPTLPSCGLLVVEAAAGADAALLSDWLALLVAPTERVAAELNGLQDRLPPASMRRLLVDLGSLLDVLPLLSQPVNGVCPVLGQLGGANSGCSKNTWRSSSVDPELAGGIAEATTCCGGDSCDSPEGPSSSDAAATDAGVLSYNMSYAETLSTLASSHGGPQCQSSSTAAASLRAGISGGGVSRMSAATADGQYSGAAVHCGTSSGRAAVAAVLTSIADDSGGNVQAAVASNGSGAGGPPVKLSAYMLANLAERAAIGEASAFGGNHVGSWSAAAAGFGNPNVRRLLHGFDTSELLTNGDDETVLQQVLGTEAVRLLLVRAVSVIMFFAERMLVDSALCVVSTLSELYFAFPDLVRADLAPTIQRGAPNLMHAAVRARSLALLLALAPCGPVLGDNVSMAWQGPGGITPLHLVALLAQPYIITGLQFGVPDIREMWHSLQDDNGVSPADLLAHSLQLMYGVTSSASVTGAVRSPCTREAGTPPTPSAASKDRLSSVLRGDQRTVLRIGPQVSPLAGAGPNNVDTTSEQGSDFFQTPLPSPSPSTSLPTVLKSCDEDSASDAVPSMKSPAASSWLEETKLAAELNGALVLSSRWSNRKMKDEDESGNGAQGGVPDMTAVAEGLNRTTESCPVASVNAGDAAAGVAAPPNVPFDSSQAALPEEPHEVGAAHELKVAAGTPGMQQQGHLPGKGLPSFSGLALNIARRLMPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5C9</accession>
    <name>A0ABQ5S5C9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052145">
        <property type="entry name" value="Mediator/Homeobox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330">
        <property type="entry name" value="HOMEOBOX PROTEIN BARH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330:SF19">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1055"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1355"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1454"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1699"/>
            <end position="1770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2133"/>
            <end position="2158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1055"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1084"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1357"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1379"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1498"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1699"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1748"/>
            <end position="1770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2245" mass="235641" checksum="58A2F16C4393FAB9" modified="2025-10-08" version="1">MMASSQVFYPFHSLDDLTNCCSGTTKFYTISLADIASCGNAAAAAEAISTWFANIPTSSDPTLLHAWPSKTPITALYYVWRALRDGIEHNNLVLLASRALQQLRQLLGKTAQHELQGLSQPRARQHNQRDSIPQPQRDKNACNDQTLYQEQTDMRRESHIPPQSDAPSVHWQLQEGAKMVCFFAAQLLILAASPASAAGDSLAADNRGRGNPVAAGRPAGDRSGCSVRGRSGRGRARQGGKRVGSREGGKPGVWSSAGGDRLARALDSGSVDARAKALQHAAGCLDDLGQLVWVHRLKFSSAMEARAVTELLVSSLLLWVPRLPAKPPAAQREAYALFREHMAGLLCSAAWQQHCRELRASQPGPEETPCQMQQQLTFGRDPTGAGGVRCSYNLRELLVELVKAARDFNPSARSAAARNSGGGGFGGAFAGASAMAGGANGGANLAATLAEAALLAFDRDPSGGEKASRLLLACSLMAATPDGSNRGTSTCGRAKRPRVEMHSAGGADGEEVQLRRLLSEVLIQAVCMAAAQLDSVPVAAAVLQILSPGFTAFSGICRKSLLDLAAVMAQERTHDANAAAPSDYQVGIGGAVGERSADVNAQAAVAVGVAVLIQGCCDADATIRCRSLSHISSCLGSLTRLAEVEADASEGGTMAGRAGTALGPCEPRPTAAVRLPGARRIADDATPELVEGIGGVSGHLGGQAVIRGLLLPALKARLLDRTRTCRTRALQLVLELLSFPAIASNELGPPTGPRLKRGAVERTPMQCRPSRLASIVARWCLSELCVLLDATYGSPLYDEGNTQLLQQVVAAMCGAMHSEALLSLLLPQAPGLHKGNTSTTASEAAAGRASRDCEDGALPAAVIHMSPSTCRMLASCLTLTVGPEGVFNTDVVKPLYEIVVRYLAPVGKGHAHDRSAACSGSQSIAELPSKLPTDWGCCPAAEAARDDDEARTRCMGMMLGGTSLHCALWQQAFRELCRYLAAVEVDDREAIGDEEREEGVEESLCMGKGVGAEGEDQARRLSAAAAAATAAAHIARAARALRVLVGLQRYGPKQVAAGTGPGSSSGAVAPLPFGAEGRQRGPHTAATATATATAGKRPEGPLPGAGGAAGCSGVELLPDEALPSPAGVIRDPRDNTDITRCSAELAIVLDVMETLLAETPPEQPQRYHHHHQQEEEEGKVGEEEMQHLRQISDASDAFAWLELERKGCLLWALRLLETGLSMPGPFAAIAPRRRNCSASLGQELNSADHMNTDANCGGGSTRVSVGSGGAVSGDGGSVHRGGQVDSAASSAVPRLDLNSSEDVGRTVLSYLDCAIPRILLHGSLSAVDASYAVLCRCPGSAPVFSRHLRAALEAMRQQQQQQQQQQQQQQHTDCQQGSADGGCAGTEGGAHTPAVADTLRMPWQPPSAIPGQHQLRSRPRPPAVVPRYLILLAHMTRFYEYEREAFIDRLHEARRGDGGSDAATAPVPAKRHDADANDAAGDVNGRHHKVIDALPPDQGDKRGDEGLEPASGKRDEDAVDEDDDYMRETEETRGCREAAQRMLDRLMESSAPATHAPALLAILPKPGMAAGVHDSKPIDANADTGAKDNKYQGGYSDFGSCTAGQPPVQLQVLALRALGRLFVLSHRLSDAHGAVLEGLLAEWRGRPVRLVCEALAVAHDLIHRDPGRHRQLVRLMEELIQAALEDWAGLGSETGAGAGAGAGAEAGAGAGAGAGAPTAENSAEPECDIGKGKQQWRRHENANGLSSRKQDGKEQAEHVDDQTKDNQERQREQKSSLHCLRCTHSLCHHDGVLAALLPTAVGCYCRLQAMGRLQWSAMTYRTLAMCLTLARGRLRKQVALQAASLLLRSNSVLGNKHNNRSGCNSAANTHARIPTALALLRNCPGPLRLRLVSEALAATGAPCMVTNTNTNTITSSVDAENPLPSMLRPEDFVSDHLALPVLQMVCSGDGRDTDSAGVTTAATMASGGDVEGDIPAEVVATSGSASAMSKAGVMSKASELLDSSGGGAAKSTDAANSFMAALLLLRQIIPSARALIVMVPQLQAVLRDPWILEGFTPGAVQVLRDFVLRYKHVAGASTAEGPSEAAASCGAAAEPNTCTGGPAKVPAEGMRGGGPAAQRKLLDLLVQIQRRLGAASDPNRQRTKRSAAAAQQLGSMPNGGSGVLQYERAILAFTSCQESAKGNKNDVATGRQDHRAAAAEPVAVLGPAKASAAMATRRTLEHDSKPRIIATTAVPLAGKKKRKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5M7</accession>
    <name>A0ABQ5S5M7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008267</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046024372" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="34"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="346" mass="37300" checksum="5F17D3DAD1D341D2" modified="2025-10-08" version="1">MAQRGASPDAALLLALVAGLAVLAQGSYLTGTKFPFENCVQNTKYSPFSASFYSYSVNTTRNTSTFCLTLNSGAACISGPYRCCDPTAYINKLKLFPALGCRGSVGSVNVITPAGKNYSISSVYYEQHEGLDIVKITPLRQWLKDRQSIQGVRVCYNLKRPCWNMAMFSFNKRQVEYSLYDKKVDNYECCPVGVFGLPQPPSPPPRPPRPPPGNTSDPPTPFEPPSLPSAPSPPSDPYPPSDPRAPRPPSPRPPSPRPPPSPRPSPPPRPSSPPPKPPPPVRSPPRSPPSLKPLTTSPVPRAAPSPRPSPPPATPPPDVPFFLPPDIPDLSAFPKTDPSTVPEDTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6B3</accession>
    <name>A0ABQ5S6B3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="173" mass="17848" checksum="7367780A36366815" modified="2025-10-08" version="1" fragment="single">MGKKKGKSGSKTSAAAESSSPTAPPQEAESPPALVAEASDAPSLEASTQEPATSSDTQRDILNGSPVNASSSSSEINELRLELDACKQQISELQAKNASLSEENERLKSAQSTFAVSQAPVATPVPDFEALTQRISALKAEQAVADAAREAAWTQLKSVVADIARLAAPDGVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6X5</accession>
    <name>A0ABQ5S6X5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_008379</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008672">
        <property type="entry name" value="Mad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168:SF0">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1 MITOTIC ARREST DEFICIENT-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="280"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1014"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="78"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="317"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="433"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="663"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="927"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1028"/>
            <end position="1039"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1054" mass="114051" checksum="57CBAEB6E934CD55" modified="2025-10-08" version="1">MLAEAHSRRAAPACVRGNPCHTTKKVVVNRRWIMPFVHISSSFVAPQSGRGDFTVAGAGVPDQGIYSVGSASEDALELAATKKNLREALAKIDILSQQVAIVEELQTQMLEQDELIAELEQLIESDKRKQDNEREALNAQVAAAQECLAATTSELEEARSRLRALPSIEQLQAELARLQDVEAKLQAKLNAAQMQAARLTELEALQEQQKLLQAQLQEQRDSRAALVRDLEEARQQLVAAQAAAAAARQEAQEAQEALARKVEELAAIEADIEAMRKTMNKGGKKGRAPKVPPARGGAGKGFASPIPTAARLPTGAENHLREELKQAQTELEQLQGTLDEREAQLQAAQSAVEFLTSQCKDQELQMMDLQAKLDETSAKLKERVAAEIELQQQVAALAIRLKAEANDKTTAVATLTQQLDSQCRERMALAAERDSLAMEVEGLRGRVEDLDERRWRQDAALGELDASLQDLRSEVETAQQLKIDLAILAEAFSAKEAELEAAQSRLGVAEGREEELEKQVVLAEDSLKSLTAMLSELQTTNTELAERFEALEASFRTTSEQRADAEARAMEMSHQLVEARDQAVALREQLSVKNKALAETAQALSEAQAAESSQRMAAQTLSERLAAEQAAVIKQTRDVETLREQVVVLNEQIAFLSGSESSVKKLQEALRRSDGELADLRNQLLDAQRQAQAADNSVGEKVKDMDRLQRELEAASAALVESESALALARRAAEATDSELRIAKRQAEAAATEVASMRAQVAALAGELTAVRQDRDAASRAEFESLRQLQSELAAEAVATLELLNKAESLVVVSSEQASVSNAIDVNALLQQARNDLDVQTELWESERQRSASLEREVAALSEQLRNAALGRRLGAVEESDSVAAAGGSGSMTGTLAAAFLNARSGVSSLGSAREMEAANVKLLEDLNVAKDSALSAKAEAEALQTQVQFLERELRSRDALISELRSNGVPRGSRQADNLVQQLKSLQEENTKLKQAAARQQQLLDRTKRFFEKKISPGNDSDLSVVAAGSAGGAPPRRGSGGSAGSSPSRNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7R5</accession>
    <name>A0ABQ5S7R5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="556" mass="58375" checksum="58DDFA9D319DD280" modified="2025-10-08" version="1">MSQHAGGPNSSSWDFTAASLRAQNRIEPSCPEEHQRIVLNKAYQKAIASLESAQAVIAGVSSGLYDVEASQTDVSARLSGRSTMRYTGRKRKLSPCVYDYSDSCFTGAKADTPTQDLLPASRSELRHHQLQAVRWLASLELQGAGGVLADELEQDRRAEVAGLLSHLLMLGPYPSFSEMNNGGSTRVAGGGGSQVCMLLAPSGSLSGWAEVISEHVDVRVHVHDGTADSMAALQAECTALAHRPGAAASTPGPVARTSQLPPHTGPQTGVSSAAAASRRTAAGAPLLSSSSSAAGVYPPPRHPLTVAEAVTAAAASPLMRPNVGGSGLQHRSLLVVLAPLEHLHRDAGLLTQIPQRLLVVDLAPPEGNDGEGGSGSVCAGRGTGSGDDSDGVGKTLFRIFSGDFEVGPDSDSGSHHTAQFCSGPMGRWGQGMVLDSYHHLHLLTRMPAACRVLLSAGIPEPAGDGIWLLLQLLAPRVHRPLWEAVSELQHGLVASGPLGAQCKENVLSEIHGRLWGLLRPLTLHRRARDVDLVTMRVTATNLNLYDHFSAARTDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7T6</accession>
    <name>A0ABQ5S7T6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009680</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_uL15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934:SF11">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="115"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI65988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="248" mass="26496" checksum="45D23EE09361B014" modified="2025-10-08" version="1">MQVASRALRSSVRPFTPASLRAPRALVVSVSASERLRLNNLSPEPGSRRDEKRKGRGHASGQGGTCGFGNRGQKSRSGPSVRPGFEGGQTPLYRRLPKLRGIAGGMSAGVPDFVVVNLDDLEKHFTAGEAVTLEAVKEKILSVSGRDTQLPLKVLGSGALSKALTVHATAFSESAKAAIEAAGGKAEVVPTKAKWTKKAFKKLVADMAANGQDYEKEKLKKRIDNLKAKGMYTERVPKAKPSLPARRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8D0</accession>
    <name>A0ABQ5S8D0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009816</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF0">
        <property type="entry name" value="TOPOISOMERASE I DAMAGE AFFECTED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="87"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI66106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="303" mass="33996" checksum="3A8C75FC4BB7020E" modified="2025-10-08" version="1">MDTLQLFREELDRLADELSSKTGSLIDYLSPRGSSTRAFVAIALIGVLFSFLEVSLLLLLKPWLVKLLQQPAEAKVDQKAQLRKANDAATQAVARLVASVHHGIQVPVGMLLLLDPNFRNNRVYANSSVSYAMCYLSAGYFLHDLVMCLLRFSLEGPLYTIHALSCHMSYMFGAVTGFLHYHGAAFLMWEISTPCVHLRWFMYKAGLTHSIWYLVNGLMMIVTFFCCRIAWGYVGSYYLVNDVVGEQYRPGSPFPIAATVGYCFVAVVMNTLNTYWFVKMAAIAIAVFLKGKKVVEVTSHKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S957</accession>
    <name>A0ABQ5S957_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad21/Rec8-like protein C-terminal eukaryotic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010263</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="2">
        <location>
            <begin position="128"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="174" mass="17868" checksum="8EEAF0F2BAEB0E08" modified="2025-10-08" version="1" fragment="single">AVAAATAAAGGNSKGGGRSEGASGGVSRKSSHQAGSSARGGSGSEKEAADATPFFCLKETNGLATQVLPPQLFMSRNTKVVISIFRKQLPQAQSQAPVQVAEQHQGAGHSNGGDSGISDLQEAALSFFGLASRLKRSEAVKLFYQTLVTHTAGYVVASQYEPYGDITIRAAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9H1</accession>
    <name>A0ABQ5S9H1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">VaNZ11_009877</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 13">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22948">
        <property type="entry name" value="Coatomer_WDAD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047312">
        <property type="entry name" value="Coatomer_alpha_WD-assoc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016391">
        <property type="entry name" value="Coatomer_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010714">
        <property type="entry name" value="Coatomer_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF1">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06957">
        <property type="entry name" value="COPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003354">
        <property type="entry name" value="Coatomer_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="5"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="47"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="131"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="201"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="245"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="15">
        <location>
            <begin position="340"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="17">
        <location>
            <begin position="627"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="16">
        <location>
            <begin position="846"/>
            <end position="1241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR003354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GLI66143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1244" mass="137558" checksum="573087D9BA03CE87" modified="2025-10-08" version="1">MLTKFETKSNRVKGLSFHPKRPWILASLHSGVVQLWDYRMGTLIDRFDEHDGPVRGVHFHKSQPLFVSGGDDYKIKIWNYKLRRCLFTLLGHLDYIRTVQFHHEYPWIVSASDDQTIRIWNWQSRTCISVLTGHNHYVMCAVFHPKEDLVVSASLDQTVRVWDISGLRKKTVAPGGEDVLRLPQMNSDLFGGGDAVVKYVLEGHDRGVNWAAFHPTLPLIVSGADDRQVKLWRYNESKAWEVDTLRGHTNNVSCVMFHARQDLIISNSEDKSIRVWDMSKRTGVQTFRREHDRFWILSSHPEINLLAAGHDGGMIVFKLERERPACATHGNTLYYVKDRYLRTYDFTSQRDNPLMSIRRAGSAGANQGPKTLAYNPAENALLITSDVDGGSYELYSIPKEAARGDTAPEAKRGLGTSAVFIARNRFAVLDKATNSIQIRNLQNEITKKVPTPCATTDAIFYAGTGMLLCRSEDKVTLYDMQQRSSITELATPFIKYVVWNNDMSMVALLSKHAIIIADKRLGSAQTVHETIRVKSAAWDDSGVLLYTTLNHLKYCLPNGDSGIIRTLDSPLYVTRVVGGVVHALDREGKNRSIQVDPTEYMFKLALLQGRYDAVVNMVRGGGLCGTAIISYLQQKGFPEVALHFVTDERSRFNLAVQCGNIEVALQAAQALDDKDTWYRLGVEALRQGNYNIVEFSYQKTKSYERLSFLYLIAGHTDKLRKMLKIAEMRSDVMGRFHNALYLGDVKEQVRILEESGQLPLAYVAAATHGLSDDVERIASKLGGALPELPDDDLASLMQPPVPILREDNWPLLTVSKGFFETLAVKGAAATATSAGAAAAPGVGKGRATAAAAAAMELDESALEGAGWGADELDLGMGAENGEAEDVLEGVGGEGGSEGWEMEDLDLPADVVAEAAAAAAGGAAVPFTAPAPGVPASQRWLDRRTQLAAEHAAAGSWATAMSLLHRQLGASNFEPLKPYFMDLYTASFASLPGLHGAPSMLAHIDRTWTSDASTQSPTSPTLLYSLTALEDSLKSAYKLVTEGKFSDALRSFTRMLHVIPLTVVESRKEVDDVKELLTICREYHMALRCELKRKELRDDDMARNAELAAYFTHCRLQPVHLALSLRSAMTIFFKLKNFATCATFCRRLLELNAGAKIAEQARTVLAACEKNPTDAVKVNYDPRNPFDLCALTFTPIYKGSKYVEDPYTGARFVPDCKGEISPLGDFVRIGSDASGLYISSSQQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAC4</accession>
    <name>A0ABQ5SAC4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="264" mass="27529" checksum="F52503FE46693B7E" modified="2025-10-08" version="1" fragment="single">MDAGTPPLPVTPAYFASCCISGGAIAPAPIANADLAQAVETDASISDASGCFPSSSFITSFVGNTPFPTVAPKSGQPVGGHVKQKYRLVSPLFWLIPPVAAFSSEGGLLCTDSVHALALARAAAGRRRMMAALEEARRRFSHLSRSKLPNSWPSTAPSISTAAQELQFQREGSSREGATGSRCLDEGSEMQRPMVAPPARLLPNLPLAPRDVGILSVGYAASIAVVPCEASKSVSKVPETQQGRAGEYGRGNQEGQYEMGGRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAF0</accession>
    <name>A0ABQ5SAF0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amine oxidase</fullName>
            <ecNumber evidence="11">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_010924</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="11">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="11">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="11"/>
    <dbReference type="EMBL" id="BSDZ01000047">
        <property type="protein sequence ID" value="GLI66897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049948">
        <property type="entry name" value="Cu_Am_ox_TPQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015802">
        <property type="entry name" value="Cu_amine_oxidase_N3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008559">
        <property type="entry name" value="PRK11504.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF86">
        <property type="entry name" value="COPPER AMINE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02728">
        <property type="entry name" value="Cu_amine_oxidN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01164">
        <property type="entry name" value="COPPER_AMINE_OXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="13">
        <location>
            <begin position="14"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N3-terminal" evidence="14">
        <location>
            <begin position="129"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="12">
        <location>
            <begin position="254"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI66897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="704" mass="78219" checksum="8C377C4DEB74CC29" modified="2025-10-08" version="1">MATQTQRVKVVTCHPLDQLSPDEIQRAVEVCKEYGDGQGVGVLRFNTVMLREPAKKALLAFEQGVGPRPERIAECILIVPSASIAVEALVRLDLPAVASATPTLGPTGRGAEIESWKILEGLQPLTSPDDNTDAEAVVKSDPRIAQLLKERYGIVDVRNQLICDTWACHNAPEHLNHRRLMQGFLYLKLDGKYDNEYAHPIDMVPIVDLNLGKVVHIDMYDKPSTVNPKSVNYHPSLMDPVSKPWRNDIKPLYVVQPQGPSFSLNGAELSWQKWRMRIGFNGREGLVLHGVSYTDNGRVRPVLHRASLVEMAVPYGDPNVPYIRKCAFDVGDYGLGLCANSLELGCDCLGHIQYLDAIVNNAKGEAVVIRKAVCVHEEDAGIMWKHWDCRANHAEVRRSRRLVVSMISTFMNYEYALYWYFYQDGSIHFELKLTGILSTSVAPHGEDDPPFGVRVAPGVNATVHQHFFCMRLDPAVDDMYGGRDLVVSEVESVPLAPGVPSNPNGNGFKMVETELLSTTAAQRTHNFFTARFWSIKNPFSINPISKRPVSYRLLPAASPAMMSHPSSLVSRRALFATKQLWVTPHADTQRYPAGEHVVQSNKCMGLAEWTKKDLPLRGTDPVLWYSFGVTHAPRVEDFPVMPVEVCGFSLKPDGFFACNPAVDLPPCRDPVSTEQLPQQHDQHHQQQAVQASAAGSCCLLPSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAL0</accession>
    <name>A0ABQ5SAL0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="14760" checksum="7831D1D18E55F506" modified="2025-10-08" version="1">MDEEPPFNLALNVYKGPASIPHASAEVFGAFFLATNTALLAHMFPGKFFGSELHVRKWDPDYIAACCNEQGMRREALQGKKPHMWLLGGGPRLVNDSWERMWWDNLHWKRWKIPRTGPAFPQDMYWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBI9</accession>
    <name>A0ABQ5SBI9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inhibitor of growth protein N-terminal histone-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF42">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inhibitor of growth protein N-terminal histone-binding" evidence="3">
        <location>
            <begin position="11"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="365" mass="39996" checksum="6BC245345190FDB9" modified="2025-10-08" version="1" fragment="single">MALHSSANVMYLESFVESTSSLPTELQRILNTIKALDEKCNDLSLELQANVGVLLAMPPTHQQPTVNPEYEELVSRIGGAQKLLVQFAEEKVQLAQQAHDLLEVHALELERVTDDLDKELRRNNPDASLEYLQDFALDTTRGKTPRLEEFNMSLQQELSLPPAPAPAPKKAPAASNIASKGKRPRDEEAGGAGVGGPGGGASVAIQQPPAKKKTTVASMQLSTQPSSFEEYAIDTLPSYDEPVATEAPVPFMRPPPAGYKPSSTRPQAATGGLVRYLCPEDIREDLVGRHAELFWPDDNLWYLIEIQEVNVATREGRVLYSTGDFETLNLEETARDMHMVCMPTTAGAIALTTQLQKRSERLLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBU2</accession>
    <name>A0ABQ5SBU2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011327</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22984">
        <property type="entry name" value="DD_CrRSP7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="95"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="200"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="236"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="288"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="395"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="438"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="502" mass="55197" checksum="C5430363027EBE8C" modified="2025-10-08" version="1">MSSTYQKPINIPPAFPAILKAFTREILRAQPANIYEFGARYFAGLQGGETEAEVLGRLEGAMQGHESTSTSQVKGGLGVEEAAVMIDIATASSADLEPVLMKLFIEADRDHSGFLDRQEFTSVLRHANLRLTDRQIRQILAEADENDDDVIQYKEFLPIMVDILQSIKAKEQAKAMMHGVETMVRTEVETMLLHGLPQEELQALMLKVFKKADADGSGQLNRHEFKEALKAAELGLTRKDINLILSHIDVDRDGLVSYEEFIPVCFQVLVERFKDEIVVNDILGNTDELQQMLLTAFQEADADNNGLLTQRQVKAIFKELSYRALGLTTLQTVSLISQAPTTPDGLVQYIQFVPLAASIIRSMNDVETMKSRMSAIKAVAEAGGIQALGALDLDTLRAVLSDAFQRVDEEGSGQLTLPQVTEVIESLNSLAPDANLALSDQHMKTMFAAIDADESGTVDWTELVNFLCDALEHVEREAYIASMGVGVGAIAEGGEEEEMDDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBZ9</accession>
    <name>A0ABQ5SBZ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription initiation factor TFIID subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_011264</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07979">
        <property type="entry name" value="HFD_TAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003162">
        <property type="entry name" value="TFIID-31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051431">
        <property type="entry name" value="TFIID_subunit_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068">
        <property type="entry name" value="TAF9 RNA POLYMERASE II, TATA BOX-BINDING PROTEIN (TBP)-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068:SF4">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02291">
        <property type="entry name" value="TFIID-31kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15989" checksum="674395F596A9CF4E" modified="2025-10-08" version="1">MDASRAGAMAADNSQPQDVATIHQLLRSMGVDEFEPRVVNQLMDFMYKYTTDILLDAEVFSEHAGRAAGHVDLNGVTLAIHSRMSSYAQQPPQDRVAELAKLYNDVPLPDMVVKPGLPLPPDVKQVTAPNWQYDPTHQYQQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC20</accession>
    <name>A0ABQ5SC20_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="445" mass="47749" checksum="942F8BC8CE786419" modified="2025-10-08" version="1">MPLQHHVHRPCKSWAGVWAASYRACAHVPCRPEKINWLLKMSPYSGVSTAGLPSAHAHLEKTRLVGLALDNVDEHVSTVDWADGDVLELRLADGRHVYAEVLAGTPSRTKDARLLSQSVGTPDTAPSSSQILCGGVRYTVRMGATNATEGGAAAAPPEAAGDIRPVLVLDAVGGALSSSEPLRDLIAVPPKKHAPALRSWAPLTVRLMKPTSPQHEQQATYPGSLHGFNVLCANRVMTLRELRRRAAAEPGAWCDGSGPSDGPVSEDARAVFTAGEVAALMEGRRGVVLLQLHVGWEEHEKVPPYGPYKPLVLRLLREALEQPDIVSYASSAPVPGTGAGNPTYGLTMILCADKEPFRSWGAHLASFGCQAALEAQSPYYKVLIGHILGYRLENISHYVQEKHGQPPSSEVVAAVEQQLRRLSPKAAVLPWNTSSRGGGRKKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCB4</accession>
    <name>A0ABQ5SCB4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020339">
        <property type="entry name" value="C20orf85-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14945">
        <property type="entry name" value="LLC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="149" mass="16565" checksum="279888EFFC3AB1B5" modified="2025-10-08" version="1">MDAAYKSKSTPVADDWNWTTRIRNELGANKAWQRNWGFLLEHPSGPQMSQPAGGGGQDALTAFMQNASQAGSSPVPLSYIASKQRDSARARRQVQNDSLDGFVSNYMAKASPMARELPTKEFRKPLTTSHEYGWGRNLEVFGKLTIMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCH4</accession>
    <name>A0ABQ5SCH4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_010873</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000045">
        <property type="protein sequence ID" value="GLI66876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052483">
        <property type="entry name" value="bZIP_transcription_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46391">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46391:SF35">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 34-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="183"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="284"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="212"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="299"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="314" mass="32821" checksum="C519D3D38E72EC89" modified="2025-10-08" version="1" fragment="single">MIGEQAAKDPIGNNRRRIHHRKSASDTLAFAASAFFNELPSPMPQPFAVASMAGPAPNRSTEDGGVERPPYYTFRTGFSPSDILEVLKDMDEEELEFDVDNLCVDDLDVEGEEDVAVRDVGDADPDADADVDLDADVPGFPSHTVATAEVEATLGLAAAAAPAGPATAVLGAAIEAMEQHQPLLSRQPRQQEPQQDPQLCCSAPDTRLGSSPCGGGGGSGYVSGGSNGRRRPSGTSTGVSGAPSAAPGVGAARSSRHMRHPSDPSAWAAAPLLVDKPLSEFAGQTRANALDPSQLDPKRAKRIIANRQSAHRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCS3</accession>
    <name>A0ABQ5SCS3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011988</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046968704" description="Guanylate cyclase domain-containing protein" evidence="3">
        <location>
            <begin position="32"/>
            <end position="3580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="557"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="4">
        <location>
            <begin position="607"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="861"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1065"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1184"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1409"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1500"/>
            <end position="1524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1627"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1671"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1738"/>
            <end position="1761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2029"/>
            <end position="2094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2502"/>
            <end position="2611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2883"/>
            <end position="2930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3132"/>
            <end position="3174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3230"/>
            <end position="3250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3272"/>
            <end position="3303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3443"/>
            <end position="3505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3556"/>
            <end position="3580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="862"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="876"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="894"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1195"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1418"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1435"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1474"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1671"/>
            <end position="1681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1682"/>
            <end position="1694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1738"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2520"/>
            <end position="2531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2534"/>
            <end position="2543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2571"/>
            <end position="2587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2892"/>
            <end position="2903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2913"/>
            <end position="2930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="3145"/>
            <end position="3157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="3272"/>
            <end position="3298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="3445"/>
            <end position="3455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="3457"/>
            <end position="3483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="3571"/>
            <end position="3580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3580" mass="364121" checksum="0373F59AE65B4F24" modified="2025-10-08" version="1">MIIERCSALKPSNFIGLIVFLVTLGISAGKGAPVAVSTEAELFSALWDASVTDIILSGDVHVRSSQLWSHQVTTVPTPRPSPSPWASLPAPSNRPVPIDRQLQIRGNVSSFAAPESYLRTDAARNLRLPLLDLSDVEGWLRLFPNATLRLRSLHLSTNLSAEQPDLRPYRTLLRALLAPPPLITVDPDQQLLLTAVSLEDCLESVNVGLTQGLAEAAIHGLPTLPDAVAAAAKSAAEAATAFPSAAWAALPSPADATVQLQTPNEQLCVVGGNSGGGSNSARGPSETAQHRRAQEALLGWPLGVGPPEPEHTLQGRPWGTVVRWLTARLPRGFMGEALCWLTGRTASTAARLPGWAYIRLQLLCPRLVIGGAGGDVPATPLDSSWTGGHTGRMALSESSADVSASALKGGASCMGGSVLVGRVATAMGFQVMDHGSRLVAVTDGVLVGAWNNWANRTAGGSESGGYGHYSAGPAISPVLLLLLVANSFSASLTQVDDACWDVADGTVAACIGRTATMGITERFSPSPIPPSPLPLSPPSVPVLLQAGPGAGSKVPAVALWVVAATAGVLLVVLVPVVVLYIKLRRLQRRHIQEQGAQRAPGVGPLTTLLVTDIANSTGLWESLPEEVMDQALKLHNTCMRALLVRFNGYESTTEGDSFILAFHYPADAAKFALSAQVQLMDVMWPAEVLAHPDGQEVWVVPRSAELANGGSLRHVSHNVNAPVTLESGGATGTTMSVQRSSGAIMDGGGAVQRTTDVGYGSYGGGGGSADTGAASGFLRANWVSRVKSPKVVGVTSLHAIVRTGTVLLSGAARSLELDRMSPSELDAGEMGYGAESRTGSGLLRSFTSRMTTMLGLVPTAGSGAGSGAMATGGSGRPSSRMSRLLRMSSGAAKSSEDKSSSVHYSSERRLLSRLPASGGRRRGGVAAMEVSRDTMRSKSFGGRLLAGEGMPAMPPRPSAVAGPGSGSHGIAVAAAMGTTQSTCIPTGSFTSGAHSLLRQALSFRRRQGSFRGPSRAAAEAAAAAAMSSSAIATSATAGSSNIVPGVTPVITATTITAPCPALPASVGSPGDSRYTSSYLPPDDDSTVPVELATRRVTGQGASPSSCDSVIGGGGGSASNSNKGGLFLNGLMRLGRGPAISAGGASANRAASLSSNLLRTFPAGSMLAMAGSNTTSGPTAVALAMSSDGRRRRPVVRSSGSGGVAPAAAGSRASAEGAGGRVAVASPSGGYQCHRGAHTHGLYGDVVAGVAYGSGVSSTPRQRASYDGPLTGIGTVMAATGSTVGAMASMARETVDGRGGLSASQVGALGTAYESEVTTGPLFTSTAGAGSSNASGCLAGDEARRLSNVSTALVMDALEANGSIRPGGAPVQSMAPRVPHFRSLWIYEGARADPSRLSWAALQQSLMRRSSAPADMGSTTPTAAPATASQDLAGPLPVPVPSLPRPSAPEADDAGEGGDGSRSMPECAADRAGPAPGGGIGRFRGRGSIGDAGGAFGAQISSSLMRRRDTRGGTNSQVRGSAAGAHTLDRSSLVVNHNQLYGTDSDMEHVQIGSALPASDSIKLLHQHDDLVAEPCLELLQMAEVGPAAAHNGNAKTTGTATGTEEITLAAAGAAAAGGSSPLPASLEVAGTGSGREGHKMTSTTVGGSGSLGLISSDSAGPRSFLMLPSRTVGSPGVVSPTSGGGGVGGGGGGSSLSAALARLMRSRGRETRMHRSPTGRSAPLDSDVAALSDYNSAGMSSLAPTQEPNAVDKDMHPHPQPNVYLRRQLRASSSGTGPETPSFMHGRAHAAIAGNGRTSEGHLQAIGGFIGSSGGIGSYCRGSVGTVTTSPVAGHSPTASRTGSMTFGPISQTVARSWSRANILVRQLTRDRSISRAARSAAAGNTALDISLGNISNGVIGIPNSTGDGDDSGGRTAASGGVGSGAASGLLLAASARLAASDPEADSGAELSAIGILADGLRSRVAAGSVSVSGAVCKMHALFMASGGGAGSGDERQFTPRDLEGGARHRLSTRMVTIDLGAALATSAAPDEVLTRSPGPASAKFPESPSPPPTLHDPKSKERVGAVQLQALPHGGGGESKASARGRAGPISAPPKSLANAYVGVASRRISFLGLGLAPRSRTVERRSSATEEWQTAQADVTMPWLGGTVSQTGALSQSLQMPVECRSWRERCREDWPPAVRPAPPARCAYRGLRVRMGLHTGIVTASDVTFNPTTSAMAYGGTAMKVVKAVGDAAAGGMILMSQSTFQALVSIMMDLPGSPQAIFSGESDLDLGSDAANYCLYQLVHRELVQRIGYSAPLRNIPLTQLGTEDAPTGFCAISFMHVASASMLVAELGQPGVEALQQFKSIATGLCSRCGGYVVEASEGLCLAAFRHAAAALVWALASREALTSHPWSAEVRRWYRFALANNFSSRGNRGPRPRTGVHVGGVNVEVNAATGRMTYRGKVMNRTSRIAHKATSEQIVCSKEAWDAVEASLQRMVADIQEEQRREDEALRKVAAAAARGRATSSTVEGYGDGNGDDGFADDDMVSELGGGGGGGPGSAVNSIRALSPATTTPSLRLPPLGGGTAASTHGSPASTRASGGRQTAAGRKGSLGSKAGSAPPSARSTMVMTSAHQHEGAASDGVGRIKASALAVSLAASVRAHRSASAKQLSAAAAAAAAALSRRVTSFTIRGSANSFPELMPAAAAAGLANGSVGGPVPSVTIAATVTGRATGPASANRLLAALARVPSSPPRLPGSTPQMRPSLSSATVLNPVPCTSPGSVGGGAGNVGWTSPMGRPTSAAVAPNLPLAFQSVASEPAPAGSGGTVAVSITGGDGGGVGSPPALAPSLRWQALSRLVGPKVQLPVAKRLEARYLGSYSLKGVREDMKLFEVFWGEDEDESIGGSGGDREGDINKVEVDAGGGDGAGRSSQRRATSSPTNLGSSTMGIIMNVVPAETAAAETATGVAPPSVLDGKERMSIVSAARVGGVKSLPQMSMGTAATPNTASPTGGFSGGTRTIPAASAIAAPGLAMEAEPVAISSPEFWRTRSGKSDWQTIDTGCSVYDEDITGGVLQPADMADTAAASPAAAGAPPIFHDVVPRASTAGGVVPNGSFRIGGIRRSENGRNNVFFPAVGDFATMVPELERKRDQDNGDVEDDGGIGGGAGGGGIGGQSPRAGPAGSSGAWQQKRQVRLPPFPSQLLPQPSHLQQQSGLEAMAHRPSAQVTIWPVPAIVEPPFAHIHGSYPGSPTIQQQQQQQHDFPHYPSQQQIRPRSALRHDLQVNVTGQSVCSGTSPGSHFNGSAGDISTSSNASNRRRHPAGTLGVTFADPPLRELNQVQLQGPHGVSQRAIPCANRASRNDEFQPGAFTGQLDVGVGLDRPQAPEAAIPAPAKMEAPTVGLRPLSAPTPVRVLSSQPASIEQVMEPQPVRPQPLTAWGSSQAALGAEASSSRIVGDVHTASPSLSLSPPNEASERSPRTDLKSDHDAPRDEGSLHHEGPGLSQGRPLNPGPPQPSYGEGIPWAYRVGVGARNGEAEPTPSLVTVQQQRRAWEGTGPATIVDAAGPVVAARGALPPTSTSTQAGTMEIEEAVEDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCX9</accession>
    <name>A0ABQ5SCX9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011463</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045237765" evidence="2">
        <location>
            <begin position="37"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="615" mass="64532" checksum="97D9BCD9CAB16719" modified="2025-10-08" version="1">MARSGPAVMTHGSMVAALVSMALISLLCCGTGGASAQEILEIPNAFPPLSQPWVSPLPSLPPPSYPARFPPSPSPSLTSTHPPSYQLTSTHPLSYQLTSTHPPSYQLTSTHPPSYQLTSTHPLSPSATPSFDMDLIATARAAEGADIPGTSGVFNDVSDFRSAVNIPNNIPVNISVDIPNNIPVNIPVNIPVDIPVNIPNNIPVNIPNNIPVNIPNNIPNNIPVNIPNNIPVMDPADALARVVEAINDLTVLAYQKKQPRPPPPPTPPPPPPPGSPPPSPPPPLPPPPSPPPPSPNPPSLPPPSPPPPFPPPPPPPPPPSPPPPSPPPPTPPPPSPPPPTPPPPSPPPPAPLPPSPPPSMPPPSPPPTPVPFPPSPPPRLPPSPPPPSPAPLLPSPPLPRPPPPPSPLPPSPTPPPTQPPPSPLPPQPPSPPPPSPPPPAPPPPSPNPPSPPSPRPPSPEPPSWPTSPQPLQPAFPPPPGPPPHPPPPPPPLPPPPSPRPPPPPPPPEEEFVAFKTKKYIYILSTKQYNYNDAEYFCFDKGYDLIPYNEQKHNEATDRLCYNPGRGCWVNGKQGSYCAYIDPRGKGGSYARYCDELHYVVCYGKNPLRLRAAAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDI0</accession>
    <name>A0ABQ5SDI0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011707</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="171" mass="19450" checksum="884F417CA01009EC" modified="2025-10-08" version="1" fragment="single">MIRIFVYDHFSRRRLTVMLSRTCAPTPSPSTSLQQLLWPCRDSDRSCCPRDLHHITSHQINNTDPKVPTKIWGPVCGITNFSLASDVARVACGQRYRIQHLPWAGDVFITTTALAPRVPNYILIQAVSPGDFNPAAGKAYVTITGRKRDNPTKLQDHKYTVREPRYLGYIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDS9</accession>
    <name>A0ABQ5SDS9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011676</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045198308" description="SRCR domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="4">
        <location>
            <begin position="34"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="4">
        <location>
            <begin position="191"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="374" mass="40429" checksum="5984A4063BABF3FA" modified="2025-10-08" version="1" fragment="single">MRGAAIWLALVLMIGVGTFCQGQRNDSAPSLYEIRLVGGRSPSEGRIEMFDGQDWGTICDHLFEDADAMVVCRQLGYATGVAIEHWGGGAGPILMAGVECDYIPGVVPQTLHQCYSVGWDGVANCRHEQDVGVRCQGRVNPPPVLTPAPPRPARGMPKLPLAPKLPPRTPPPRPRSPPPDAIPPAAAKYDVRLVGGDWPGEGRLEVFDGRQWGTVCDDGFSNLEATVACRQLGYDSGVMVPSDRVIQSWGMGTGRILLANVSCSQQPRTARLRSCAYQSWENTAYIDCYHTEDVGVRCYGKGTPRSPSPPVPPYPPVPSRWINVAAGRPVYASSVLAGDPAVSLCENAVCRPSYVTDGNVSNPLRVFHSDQYDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDV7</accession>
    <name>A0ABQ5SDV7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17736">
        <property type="entry name" value="BRCT_microcephalin_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022047">
        <property type="entry name" value="Microcephalin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14625">
        <property type="entry name" value="MICROCEPHALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14625:SF3">
        <property type="entry name" value="MICROCEPHALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="3"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="1551"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="1672"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="640"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="814"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="855"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="956"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1154"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1195"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1315"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="966"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1339"/>
            <end position="1353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1760" mass="183705" checksum="B3FF64ED8A30AED4" modified="2025-10-08" version="1">MAKPQGILEGCCVHLWKRVDGDGLSLEGITAKVKELGGKVATKLTKEVTHIIFQRALGSSVKELLAEDEDLRFIYRKIERSSGRLVVVSPLWLRSCETVKARAKEARFIVPQPKKPIFLDHIERGENKRKRRKSYSARPREQVEVPDFENALFSSSQQIRDLSSFPDCQINDGRTNREDDKAMEQSAIEGEMQPPRLRTAGPPNVPTGPIKASSGLRGVPVRLQQSSSSKRGMRQLSLNFFVLPKKPRLSLAKVTEPYSAMQRPTAELEVQETCAEDKSTAAEVLPKARPEFMLGGGPSPAAPGAVADHGDGELRGQQPEWLGKRSLGGESSAAMVTIAVCEHVTKPGTPTLASLANTPLASAAPPRVQMLQVTDRGKSCAAAAAAVAAVALEPVAGAPEFDKVGEKPAAAAAATLPPGGTEARRSSTRDAPRLLAGTPLALGLIKSLGRLTPLTTPNGITPGGLVVPKLKLQGNVATLAATSGLPSGPDIPSVVKPIVESPPGLARCNRGSARTLAKLGADGLPASIPAAGHEPVRGLESETSAADTHSGAPPIVPVSTHRHAKIRGLLPPTPPVPVQMGPERARDCSKAAAAGAVELVKTESVSDPGIFGSFLGPNGSQTPVLCNAVVPATFSKAMLGGSGDGREKEAESAGGHDLDGTVEEEHGGGGVAGTAVCRRSQRRTRATALKSLHQSPVLYGGTSRQTLKGEAETASARIPAAEQGQPHEAGEPAGLVTHAEQEVAPSTLAPPCPGLTPSFKLRRSARLVSLSPSIEVLPDRDLVNEHDAVPVTDWSSRPTGSRSQRRHRQELHDFTWGGFQPPHQHPNLNPGPPPSVRKLCREVVSLLQEAPCTAAPWPEDRAPAANCTPATARKTRRRKQVADEEEPSTRSQQEVDLNPGSLQEGPRHHDAPMLLAPYDLAVAAPSPAFCTRSRTRTAAVTPRLVPAEAAEQPRKVLGVPSPPHQADGKWPEGRSEPCNNGVRRPLPQTQPEILHSVPGGNPSYGVRTAGASGLSLPVGDAAPSVLKRQGTAARSRRGALNSGLACAAQAPPGTALKVGSTGLTDLATALEAAAESPICAVVQATYAAGGDLRLEEPQLPPVGRAEGPALAPVLSLPPYSGSTVCENTPTDLSHAAAVVAGPIPALLRDAAARTNSETTGVTESHGPSGPHPMRAEPKGASPAAGGYLCVSSSIGVRSPPLPPRPSMQKPQSEVPADELYAPGKGSEDSDWTREPRIETRQGQITQCIGASSMDGSVDGAHTDRGSMGATGLITSAMPKNVTATMMIDTLAAAAKPTSVACNFLVSISRSRLRLRKQEAADQEPMAHLGGEAHGGSGGPQAATASTAAALPTSKQPAAMLRRNLSTLREAPALEAAAGVFELGGGDRPESVLLSSVEATAVLSGPSQHTTAREARDTEEGKVAATKACRAAAKKWKLLSVSGQPSATAVVNLQGVTSRSMVLPNAITGATAMACRPASTCLGEVGNSKLNRTTPAAVPDVVPSSRTKNPEAPAAWLAPALGQYPRGKDHPMRAGYRYLACTKVGEEMEATIEKAVRKLGGARQCTPGYEDGHITHLVAGDASTRTLKVLLGIANGAMFVAPSWVEASLAAGEWVPEEQHLLQGTFAEAANAVRASMQAQQQRGVGARGAPAAEGAAAAVVSVSQPAEPFAGIVAKPLTGRMVAVQNSQCNGPRLADMQKKKAKLETIVKALGGRLGPLRSADLVIMIRGSTLPQTSRRSVKVASEEWLVRLAETHIWQEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDZ2</accession>
    <name>A0ABQ5SDZ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012484</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF11">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045712989" description="HORMA domain-containing protein" evidence="7">
        <location>
            <begin position="22"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="HORMA" evidence="8">
        <location>
            <begin position="63"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="254" mass="28624" checksum="E95204C99B8DEA9A" modified="2025-10-08" version="1" fragment="single">LRLNLPFLLLCINARIKETESNPHIIFTTSYFPSFHGGPAHSQVIEMSLQCQTQLQRSTITLKGSAQIVAQYFEYAVQSILYQRGVYPSEAFKQKKEYGILLWVSNDESLNAYLSTVLMQAKAWVESGKLRQMVLVIVDASTSEVLERWTFDIETNQDVLAGGKPPEKAESEIKGEVAGILRQICASVSFLPLLDTRCSIDILLYTGNIDEVPEDWEDSDARNIKNAEIVSMRAFNTKVHNVKTMVAYKPEDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE24</accession>
    <name>A0ABQ5SE24_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="35866" checksum="126058BE0EBF97E0" modified="2025-10-08" version="1" fragment="single">RLLNVPSRLTELQRHSVRMVITVHVVPTAEQSVYHDPTYLSMTERNSLEYILLDSEYQLCLFWHRVGYHGRLHLAELAEPIRNRDRSLLCVYAAEASVGTIAATIPVVALGGVAAWRFALYGQLEYITAAMLSNYVPKGEPGPRVIQLQGGTRELYYYPKSTVQVTVVGENINKGLVEQAGLQAAVPTTAKMQSPANLGFAADTSVDAVVSVGAIAGMSESQRVAFATEALRVLKPGCPIIFVERTESHLQGWKERRDAFELVQWDVALEGQDPHAVGVAVKSAEYRAPVLSRRAEKAERERADKETRKEKARLPKKGFEKGGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEA6</accession>
    <name>A0ABQ5SEA6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="219" mass="21798" checksum="BA43FAD06CE58569" modified="2025-10-08" version="1" fragment="single">PSPSTDGSVNVESSPRPVPTPRSDTSPPSPPSLASPPPLPSLLSPPSPPSPSNDGSVNVENSPPLAPIPHYFSLSPPPPEKQTTLLLQRPPIYEVLVAAPPLIEEIGNARGNGTGNSSTQSTGGDIRFGSPPGAATGDGGTGKGSVAGAVVGGIGALTVLAVMAVGVLLRRKRKNFTPESGLMSDLMDEGPAPPALSAGPAIIAVNAAVASQALLDARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEJ2</accession>
    <name>A0ABQ5SEJ2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="243" mass="27073" checksum="0EDB4777B6EADBC8" modified="2025-10-08" version="1">MAFLGAEAQRRQHHVGLEKYKIVHHDFNHFTLGPSKGKSSANKMAFTLVEPEEFQPSFNVLYVRTQRPAVLRAPLVQVLLDTWAEAVELVRPVFRFGIPESSLKDKCKYWRDDGSSLPTTPSLQTQRLIKAISNEAFGASRLNRHHHHHIAQQPQQPQQPQQPQQPQQQQEDGAKDGTFGAGGHGHGHSHGQGGGSHIGKHHLTPFQEKEEFVETVTPWVVLGLNVAAWCYLGTSANNPTPGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEV4</accession>
    <name>A0ABQ5SEV4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012879</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146:SF3">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="46"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="479" mass="51580" checksum="55841F6654187587" modified="2025-10-08" version="1">MVQLGQAVSVSGLLLFGTTTSLFAKIVYELKANGVDGQEKYFRKPWAMTALMFMGMSFCLPLAYYQERRAKKRSAAEDPLLSFDDTHAVSPRRSHLMEIALLSIPTAFDLVATVLMNIGLLYVTASVYQMMRGAEMLFAAFFAITFLGRRLNRYHALGIICCVLGIALVGTSSIMSGEGSSTHKVTTEEMLLGMGLIIMSQAVQAAQLTFEDFFMADLNIPGIKIVGYEGLLGAVGTVALMMPIAYYMPGPEGEGIHEDFLDTLTMIGNSRALQIVLAVDMAALLLYNVSGMAVTGHLGAVFRTVLETMRTLFVWLVDLLLFYTPLGVGQLGESWSVYSWLQAAGFVVLVSGTLVYRHGDHEANRRMLAEALALRNAEAPLEAESAGPGMPYGAAASLLPSYGAMSSPLSMAGYGGMSQPIDSLVGTPSRMSFRASQTIVSGSYSRSFHAPGSYLSRSPWSRSGTVGNGLRVSTNGIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF32</accession>
    <name>A0ABQ5SF32_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012616</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="3">
        <location>
            <begin position="1385"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="253"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="340"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="377"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="667"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="899"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1222"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1278"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1618"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1677"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="612"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="211"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="382"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="925"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1306"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1316"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1710"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1760"/>
            <end position="1778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2048" mass="206394" checksum="7E569D99AED6158C" modified="2025-10-08" version="1">MTATTDTVEVQNLVFSRWIEVKHKAEPHNYIEFFLVTDTGNEVLAISAEDHGNSHYVYHSTGAFAKYGTLNSKNRADTISWLEMIVKDSQIKVDSQLAAEVADAGKPTPDNPLGLYYTQHKVEKTANPDGTHTQRFFLLDQFGNWHLAVVGEERETRDGHYLYKATEEFSPRYPLSCHAQKDVIHWLEQRVTHNLPSSLASHRKHDGSGSVGTPVGVTGPAVSGPAASVPSGTSADAWGSGYPGLGAGGGYAAAATPTSGSDGPVGSDGSGPLPRANHEFKSVQQQQQQILGSIGSPVADGAVSGHNRMRQQPQQEALGAAYHGMQGDGVKQDAGEPQLATIQPAPGTTHASQSTPTLPPQHLPNCVGALAVQGTQRQAGDQAQTATLPPQQQQQLVSSGLQHFGTVPLQQQQQQDSHHHQHQLPVDPPGHTAAVGQSTGSASPLASLQQLQNEAAGPQQHEAAATPAALPLQQGMQAPSASTPQQQHFALPGTTLQQQGTQGCQPVLAAESQGLAQLLAGLAASMPGGAPSQLPNNCPPLQLQLPLPPQLQLQGSNMGPPMASPLQQPQLQASPQLQPLQQFMAPSLFQPPPPQQQQQQQLPFPAFDPQTQLQQQQQLQLLQQQLQQRQQELQQLLQSNAAALAANNVAVGQQLLVPGGPLPLPCSAPSPGVQAGNPGNGAHTSGGSGGMPQQPQAPQLLEQSATLTSSAQAGLGAAAVQVSTSCKDFGIGSALGTGALNGPAGNALPGSVLGAPFGQLAADTITLYGPASTVAGGGAGPSRMPGQAIVAGGCVGTVTAAMGLQGSNQQPQLQLLGQAEGVVGTPGGHLGAAVVAGCGGAYGGVDALAAGGVTPLTTPPSPMRLCTVYSVGGGDGVSPGAPSSSGHLPQPVGPVTVGQLMVPGSLPPGTRGTKEAGPGPTPGSGHHHGGRGGRGSSGKHKLAGVAEVAHRRKLARLAAAGEEAVRLAQGAYLAALRHQALSEAAQQEALRKTIASWVRGQISDDEVNVNGWHENLEQILHDANRTRTMSVPFLSNESLMEITRSSVEAITLAAAVSLTAPYDPTAASSAAPPLIAQQQLSLLRPVSTPLPAGVAVQMGAAPASLANGGEGECRAAADAASADQPSTSVVCHAEALAAVSSLKALLMGDLQAAVQVPAAHTRAPAPIHGSHALATSGLDVHNNETYGGTIQMDVDSKAGEAGQAGVSAVPGVLVDLRPTVSETNEPAGLVPTASTANAGSAAPDSNPGSGHQSANAAAPAPAAHAVSAAIPPQQLPAAMSPLASPMTSIDGAGLDPPPVQPDSTISGAPGPAALPELPPQPPHAPHQQPPLPPPQPPPVSLPMVPMRPAGPVGSTAVLACAGTAKSWSFSDPYMNKHGVRIALESLRELAALCPPVANMASSNLVDTVKKFEAHPHPSVSGLAKEILKRWRGSFIHRLRCMSDSRCYQDPVAALESKIQANEIVFPSLNELLGVPRGSGAAATAADGAATPMTGVMTLAALAPGPGYTPMTPTPTGVNNGFTSGSMPYPLPTTAAPLTTTTIGEAASLISLPPPPISFTIGGAPTLMPAATTPAAGGTSTDCVTPTGGLAATAAETPRGLFDASLTRASALVSTPQLFGRHGGPPASPGLTSPGLATTEMPAQSLQYPAAASSPMSSVVALPAPAVAVAATQQQVTMQTPGAEAARQLQAAPPRSPPPLRQSQLLSPGPAQLPMPSPRVQLMPPPPASPASVGPMSTTAPRQKALSRPGAALSTAQAPSAMPVVPQAPGVAAAPPASARIPLQPPPPSNSSSAFALPSHLLSASPLVAEATTADVAALLATSAQSLAAHMQTSVAAAAAGGGAGSSLSPSISPAQLSQLVTLASTVQVYASALKASDAAGAASAWQSLVQQLRQAQALSLCHGASALGGKLPQWPLSMNPQSSLTVTGVVSAAPPQAPQQLQIQSLQLPALVAAAPEKAVPTALAPVPVAAGENLASSAPHAAGESPRQAAATQPPVAAFVVTAIGPDSRGVVNAAGGEVVSGRSGAEGVNIVEVPLLCGAEAGLTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF49</accession>
    <name>A0ABQ5SF49_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF18">
        <property type="entry name" value="ORC1-LIKE AAA ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="810" mass="84836" checksum="EF2B577E43C152DD" modified="2025-10-08" version="1" fragment="single">GGGGSWRRGGGVGHEEDGRGLVESGEGSNSKRELEGSISVSQAAANGRVIGNSAKMEGLQRNRDGGAIADASTGLHADLQGDGGGMDVYGVDSVTGSGSGVGNGGKVASVFWQGELEYEEVAVWRRDRILGRWLRRLWMSHPSTRPAIQDCLIRHLHAPFWRTVLMAIVNQQARLFGAGGGGGASAAVAAAYSACGSGRWNGCAYADSNLSRSPQPPSEPTPLELIPLTGADAFVFLYGGTAIKIFVHEVPAMRGLMCALELRTPQLLLAQAHVSATETPAEAAVISQSPVEAVSVAAAGGDVGDPTMALVVTAPTGFPPLLPLLPQPLAWGTVAVALTRRRSLRGEEEEMQDREETERRAPGRQGQLQQLLYVVHQKVQGSCVLQELLDAELSPHYLIAVAEESGHLMGKLHAAAAGKAATAAATVPAAMTAAAAAPTSVEEWDEVVRERAVWHNRYQNIWVSGLGCVSAAPDDEDEGSDSGSGGGAGSGSGADAATSSSPRTFCADGGWRCDVPLTSPWWPFVQHLRSRKRRLLRNLPGLSLPAWVKDQLPAYLPEDPAELLGFGAAPELEASASASASASAVAAAPPPLLLHGDVTAHNVIVAHPPPPPPPPPPHTGDFTTADRSADVCDSWHPQLSLVDFSDSGHGDPLYELLPVLASCLRCDTKAAAAFWACYCRHVQPPHVWPKRLVPAPPGTNANGSIQNRLSLSYCAMCYCLLHEEAELLLQRVWQWHGPAASVSTSASTSASTSVAAAVTTTAVQEGSGSEGTAEVAALGLGKVGFLDDQELAGQKTLERLAVRLWGFLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF86</accession>
    <name>A0ABQ5SF86_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012961</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592:SF24">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="3">
        <location>
            <begin position="243"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="315" mass="35064" checksum="79B79BF9087D4E41" modified="2025-10-08" version="1" fragment="single">MSSSLHQLRLASSRECSTSYSHVQRISNPLQRHVYCHKLQHRRPRITTLCSKDAAALYPSGNDGADSLGLTGRVHSEAVPEKGLGRDSEGPEKQPLTSSPQEALPKVPWGLSKVFQVMTLWLLAYIIIGHVIVPLVLSCLGVDRLELTVRNHAVLHLCLDVSQLLITLSILWSCLRPYRPRSLGLFPVRLRGLWPLAVALCCASFPLVDWLAHQSMGWFPYEPDASWASNLEHSLSVGDWVTNMAYFSVVSLCAPIWEEAIFRGFLLTSLARYMPTAAAVAVSSVVFASCHFRMQTFLPLLVLGVVFSWVFIKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFP8</accession>
    <name>A0ABQ5SFP8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040401">
        <property type="entry name" value="CCDC162"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33331:SF13">
        <property type="entry name" value="COILED-COIL DOMAIN CONTAINING 162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33331">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="606" mass="66087" checksum="AB116A811448FC5A" modified="2025-10-08" version="1" fragment="single">MDGVGQCSRHSAYVGHPDADNYSKHLLTLLAQRRELQTEDIRRLLGDAFSTAATSATSTAAATSTPASASAGSAAATVASAAPQLPPSLFSSSASATSIERERRELHVELSLGLESRSRPTMAEACKAAVEGAGKPLSGLNEAVALTWHFRARQAEAVVEQRAMLRHTAALRRQHFGHVVGQSLPAEVHRLPPYRRQLDHAGSTWQGNLRPCGCEKELALAKDYMQALQLAEADAAARLGRLTSLRCPDESDWWAALHWAACRLPASRAAERFFDRLRLLPLSHHHRFPIYEQVSGAALRRDQHAATSGLRVPPLVVTTNAESVQHLEHLLREFDPGELLPQASSASSESIPMTVATENSARHHKAAYEARRVLPRVLARHRRRWPGASVLAAALPNQQEGGGDSGGGGGGSNPASQHGETTTAHGSVAPDPLPEAVEPFSDDPACSSRDQVHVVRVMDEQLAALVPSPEGDESAGYQAVLRTLPLEVDEVLKAENDFLNDCNEALVHDRMRSLAEQHVDRWSPFATEEQSVRMTLSLANAPWVSQRLQDKTMEALYVMRFLAAKRNKIKVLHYLNAIVSLQLQLLRDEREEELLAQPFPTTAAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGS8</accession>
    <name>A0ABQ5SGS8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CobW C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013695</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF59">
        <property type="entry name" value="COBW C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="7">
        <location>
            <begin position="313"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="255"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="310"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="326"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="734" mass="75297" checksum="5CFB7CA2446DFCD3" modified="2025-10-08" version="1">MSTEAHQVNLFARYEERLFLGGNAKPKVIPAIVITGFLGSGKTTLVHHILRNRGSLRIAVLVNEVGQIDVDSKLVNAKQGNAALGLPAVDLSGGCVCCSRAGDLAAALRGLRAPPAAAAPGLSASPSFCQSSSATCDYLLVETSGAADAGPLAEALTAAGFRLDAVVAVVDAEAGRQALEHPVAQAQISCADVVVLNKCDLVAPSLGLGFGGSGMGLGALADMEDMLAEMAPGIRVVRARYGEVPLTVILDVEPVGGGEGDDSGDPPAAVAATTARDDDGFMSHESVPAGRRGTGAGGYRLASGPCTADGMAVGSSRHTKRRTQAGLATTTAHPQGSESHQHPHGHHSHNHHNLHSGPEQPVLPITTGDLGGSAASAISQAAASFGGAPQLGRHDHVHVHGEHRHEEHARLHSNFYTESLTVADRPCSLLAFADYAVGRLVKEPALLRSKGVLWFGERRGHRFTFHLSGRRRLEVAQEGPWEGPPASHLVLIGTNAAAMRRLRDAFLVEVASVMPTTKPIWALTHGANDGGPSLIGGGVGGDGGGGAGSDQAALELEAAAALIASQERLELLGCERWGLSEAPVEAPGLEGGSNGCSTKVTEGGLSAPAVSEHLVVVSGWAGGGGGGGSLLSFTTRGSALHGINSEELNAELMRRTNASGTLLLVAVTAPGRISAVTNVQARDRVISLLFACKASGAQEQAGLCVKTFAEPLLRKAYAHVHSCKCDVVCGPTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH33</accession>
    <name>A0ABQ5SH33_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_013002</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027105">
        <property type="entry name" value="Prp31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019175">
        <property type="entry name" value="Prp31_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09785">
        <property type="entry name" value="Prp31_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="217"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI68632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="488" mass="53055" checksum="7ED38E1162B3BA26" modified="2025-10-08" version="1">MATLAESFLADLEDLSEDEPGPESEQSEEEADDGMDDIEALNFDDLSGVAKLTSEPRYSEVLQRVRTALEEAASTTGGGADRRAAGPLEDDPTYKLLVECNRLAVDIDNEIAVVHTFIRDKYRPKFPELESLVHHPLDYARVVQRIGNEMDLTLVPLDDMLPAATVMVVTVTATTTSGKPLDDEALSRVLAGCEMAVQLDEDKRTVLQFVESKMNEVAPNLSAVVGTEIAAKLMGVAGGLLALSRMPSCNIQSLGAKRKTLAGFSASTAQPHQGFISSCPILAQTPPSLRSKAARLIAAKGTLLARKDAYGEDPTGTYGAEMRAEVLRKIEKWQEPPPAKQVKPLPVPDAEVKKRRGGRRLRKMKERYGLTDMRKAANRMMFNKAEEEWVDGDDVIGLGVLGKEGSGRLRIVASQQKQKLSAKAQKKFKARMYGSSGATSGLSSSLAFTPVQGIELENPSARFGMDLDTKDGTQSYFSQFGGFRSVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH71</accession>
    <name>A0ABQ5SH71_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013530</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="324"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="758"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="846"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1092"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1196"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="517"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="586"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="624"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="641"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="651"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="669"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="849"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="860"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="983"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1026"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1108"/>
            <end position="1119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1242" mass="132796" checksum="BC30A72A239DB392" modified="2025-10-08" version="1">MASNFDAHALGGRVPASRAQVMSTMAPGTAAHVVSSPPADVANSGESVIMALRHVAQQQALLAQQQLELTEAGRSEQRDFAAERQRLAQRAAELQRQAEDHDRKVAVDKEALVAREQALAARERALAAREEDHQRKVIADKEAQATREQNLERKVFADKEALASREQALAAREQALAARAAADNNALAERKQQLADAESQLQALSQKCSQEYMALLAVRAELQERLMAARLEAQAYIGGTGMGTAAGAGFGSRAVKHHQPGAAGHTSIAHDVVAAGTGSANGTSMRAWTETDVPAQTCATTDAKAAPAGVAGAVAEGEIIPNAIPIHSGSNDPVFEGRPPSKDTGRAAGGDAHGPPFRFAAERQPGKRVGAEDGGWRQEFGDAATDNGLRLGRSATESKRRPGSLRYLGQDKPRVERGPFSAAAGTSRVTQQRQVAASQSTSDGVLTVEQQQQQQPGGTGGAGAAQSALETERLAASGSTRIAVRPTTSEEAFDDRDEGEMDRCPREEEEEAAQVEAQRRMDKEKDQKIRWAWMQSQQQKPKLQKAHYQQAPALQKQVPPAATAQQLSLPRQSPPPQHLHSQHQGSSRDRDVQGKEKKRPKPDQREDDDVIVETIVETRKKRKGVDEGTKQQQDEEENSQRKIKRSKSRLLKRDQSEQGRDKKQDEDLQTPSVPVEQSNSPSPTPIASTADDEVVRAEAGRAANAAIANGGCNDRPIAAAAGGFTVQEGTSRAGGAGRPEPDTPQECATRVAEFERASTARGAGLASAEEERDAPLVPSVMGPPVQCGGSIAQVPSSASLKSEPGSMAHGTPSKPHHQSAVSILLSRAGSSDVEVVDIISPSFFRTAPTEHQSNHANRTQEGLSQQQASQQQDRPQSGPAGGSRLHPHSATTYTVQPDFPRSQGWSYGPIRSGVENFSTTAAVVGNNGPQSEPGGQAVPQPHVRLNAASVIETDRQEVEQSAASVRTIMPPPSVYISLVGQEAEVRTRKPTSRQESKGLKDTQDISGQSFGSIIAGDAGPPPPPHSSSDGTGSTKPPQQQQQEFQHQFHSHQHSAQPPRTHLVYPRNPPSRPSQRAILGDPAAANGTATTRLQEYGPVPPGPSHPHLQQQSGAQQGHAGKITGGRGVPGPFRRQRMKAAGRRGGDEGGIDPDLDGVLDEDFARTVDMLRQEQELQVYGCHIWGSTGCKLSHVLHHQRLSDTSGPREQEGQSIRWRRRHRGGGAGRQQHLTAEGGAAKPSKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH89</accession>
    <name>A0ABQ5SH89_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="709"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="890"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1021"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1073"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1366"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1423"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1512"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1700"/>
            <end position="1740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1800"/>
            <end position="1825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1875"/>
            <end position="1921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1995"/>
            <end position="2075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2184"/>
            <end position="2249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2262"/>
            <end position="2290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="715"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="730"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="753"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="891"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1083"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1105"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1437"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1891"/>
            <end position="1917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2014"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2029"/>
            <end position="2042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2043"/>
            <end position="2053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2186"/>
            <end position="2205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2279"/>
            <end position="2290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2290" mass="235724" checksum="4ACEE1591B3D9796" modified="2025-10-08" version="1">MRRPYERQSGGALKIARFVDSTGQVHSTEPAASPPGAAMAAAAATAAAGASGGKAVGGNVPDAAAAPGTGSEIDSIFPHSHGRHELKGSTQQPLSPPPPQQQQQQPPQQPGNERNHHHLSHDPQRRNRKTAEQRRQLLQQESQHHSQSHLQWNSPQKQQERQCSTTTFQTPWVDKALRTTAAEGVAIVTEAVTSAGDRNNDIIGQQQQQRRQPPSPGEPQQPQQHQHQEWQRQQSQGFETAGGGADAETEPRPDASSSWHSEGMSSDGGDSTGGLAAAVAGKSAPPPIRDVGASLKVDNNAFGSCPSRPVASPKVSGPRSDLELNMSATETTINGGSGRVGRRHEKHWRWHLTTGDGGPTGTAEGLQKRKVVGSVGGSAESLQRAAAVAAATLFHAAGTVAATHQAIDSPSSPSDEDGDGQDAGGCTARKSGDLQQQPAVGQPAIAAVGTFHKLQRERENQFGAAVAAGGSDGAAEGYNTDDDSDEEFDDEFDTGERHRRRTTPPPSTAPDFKNPHGYGDEEGRGVSGSPLSPAGRHLPSPVSRSSALGLAAGEAARLAAAGDNVELAANKGVGGAGRGMSAPTGTAGRGVIDAVDPGDSQLVAVVAATATGTSHIPVAVGNETQQHQQTAPELRPVQPQQQQMLLPQHNALAEDPSSPGVEDAVVQERRGRHKGLICCVRDCNKFARLTNTGLRRYCIQHMRQHGFTPKSYESRNQNQQRYQPPQHASGDGGCSGGLPSGNTIPTASAYQGPGSGGSGGAGAGAGAGAAGCGELLSHAFCGGGRHSNLGGDDSFTSQHGQLHVGAPNVAGGPLLDELASTYRLRGASQLPYSSVADDPVIPVPAIPGATATAAGGGTSAIDSLNATVQRHRHLQSLPVLLVAPPVSDAGLGSGPGAGSGRGSCGGGGTPQPLELQKMPQGHVLPPGPCAPNPATTAVMIDGGGVIARGSVHMPAARGPSAGSLTQQQHLEELRLLGVVTLGNGDTDTPASVITGMGGGSGIRPSASLPFATPLAILKPQRGHPSVGGAGIRSGGGAAATAAGPEGSAVLRARAAVSAGHMMVVGIETAHAGGAQEGEGGQQRSSSVNQQARVGSGAVDARIQGRSVSTEPVTTGQPSAKRIRTADGHFAIAALSDGDGGCAEAAAAAAAAASAAVVEGVAAVSHVPPGTSNTRAAGMLETAIRRSGGDASPDAAQDHAGSFLGLQHQLLLPGGVAGTSGDSAAAAAALGGVSRQQLGIRVVDVDSSLMPAVVGLVRAAGLLGGRQPPILLQAANCRDTLLPPPPPPGTLRTLFQTADGRILPGRLRPVNALVDGDVEIEDVIEGQKLNPRTRGSLGAAAAGVNIDCMLEYEDAWDELLDAAARRRGSAPAPVQPQRLAAKSESNRSGLAAATAVAAVTAAVLASGVTYAAGPAESEQLMSEGDAAGEPMRPENCFQKPKQQQQQHPWQLPLQLHPATGRQSSGQEFTVPSHIVRGPEGTAAAAITVAAAAGEQVPAVMPLIAGTGLCQQSPRELDVKPHGGRGQLYQSPQEHQQRAPEQQQQQGYRPSLQKCSDLGALREEIQQHLEHGPSKQELQRQLQRRQGLTCRQQKQQYHHHLSGKELMQGVRVKEQNTEPNKESLGPVICSTDSLPGRSGDVSHQRDAKSAPCVRSERFTPLGREVVGTEPVLGAVAPLRADTSAASAGGAIRGPFARPRVDAGGDCGMQISDDDNGRRLGSDGDGSTRGVAANSGDGSHGRGLILCAKHTRGRRSQPPLPRPPAQPFRQARCVGDDATRAGNDDAIATAAATDAAAHGDVANAISSPEDEDTTAGRRRDAPTLLSSLPRADLGDRYALQPVAGGAGGWDKGRQPPAEWAFSEHQLQLLQQSRGEMATGYSNMGRAGNTAASTRETDNHPWWTSHREQQRQQQQERHQQEQDAVAPAATLPRAHVLLGPDGSLVIVDPSDGVVLAETPAAAAAEALSDLAPTLGMQEYLRQYRRQQASQQRARVVEIQNTESGDGNPFRSTDRAAASGGGGRSSGSGGGGYASHSSVSRQQRSPPRGGGGGGGGGVARTNGTLLGDAAPPSPPPAVAVLADASSTGNLESIFAQSHQQLNRRRLVYDNTRGLNFIDGSSGGGGAARGGGDWAACDSDSYYAFAQRPPLPPRPQTELLLGCVGAETQLPASAVRPPLSLQLTPLSPHQSVVLQQKGRQIRETQQPQQHQRIGRAHPQQPEGREPDGGYGQSMQTSRAAEDEAEAGTEGSDLHHAALALSQLGVVAGRPRSAPSGLASGPVQETVDDDVVEVDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHD1</accession>
    <name>A0ABQ5SHD1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013073</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="159"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="293" mass="32577" checksum="97605A18DD53F889" modified="2025-10-08" version="1">MAADVGAGHATGGSAAGVPSSSPCPTYKRNLLAELNLMLSSPYYMLNIMLTSLYVVLREHYRHKPIRQGILFLGEVTDFESWERYVAFLCGCYAAFRFIKARSRLQGLTALLDFSQLFVAIMAMVCSPVLLAYLVIAYGLVYLLFPQPIYPLNDVLDPLTPDQLAEDVKKPGTDVTWVVLYYAPWHSACRYMAPDIADLAGRYGTDKLKFAMLDIGEYSKSAAKLGIDLSPVSQQLPTIALYEKGEEVTRVPQKKGNGALYAAGYKAKDVVRVMELDMRFTRAMQKNGEKKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI70</accession>
    <name>A0ABQ5SI70_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014338</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="210" mass="22804" checksum="FF44CDD8EE7567FD" modified="2025-10-08" version="1">MSWPVPDGASPSLPVVSLPWAGRELRRPQLVIAPNKRCRASRKASVYKLKYGIAMMAGFAVVRLAKGVVNCIPIVNVVTRPILDLFPTILVGPALGTAVMYGIEQGDLFAARHRVRDAVHHMGRELNVIVQDLSSEVQPIGPEVSRAAGALEDVARAVTDSVAELERTVIPVTEREYRRLEAHVRRAVRDYGGDDASAGRGWGGRPCVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIB1</accession>
    <name>A0ABQ5SIB1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014211</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF56">
        <property type="entry name" value="BIFUNCTIONAL HEPARAN SULFATE N-DEACETYLASE_N-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="5">
        <location>
            <begin position="112"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="448" mass="51677" checksum="D11B779339A1A762" modified="2025-10-08" version="1">MTCYIRHLRKCSSVVRAKGRFEIIVRYMLRFRYIVKHIIHNARSNIKRYAMDRIRGRTYLFRRVVLLFALSSWIKNGAALNTSIQNYLDIYKPIDYAVVGQQKVTVFPLCQCGTSTLWNILTLNSAVKWVGHGKESFFFSGEIPRTTLCDNLVEDFLTQVAVQRLKLNRTDILIGDWSATHFSCSCCPTALKSLNANLKIIVLLRDPVQRALSRFVEQKRSIRMPFHKTVENLTFPEFVELEVAALKACVARAASLQQPRPPPPEDPTQSAHGQQRPVGEEPLVGWGLGLDIVRWMEMQCYSRSNIIGWSVYDVFLENYLAHFPKEQVLVLYTDDLSTRPLELFRRVEAFLGAPQHRYDNSVTDLVFNSHDCYHWRCARKKGDINNQMSLLAVSSDEGQQQQPSRNAPFAQAVARLVDLYRPHVRRLFSWAEQGRIAPPPERWKTLYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIB2</accession>
    <name>A0ABQ5SIB2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="626" mass="68518" checksum="73FB2E504C773635" modified="2025-10-08" version="1">MSSGKPFGSSSPARVARVDAARKWVAEETRAEAKEVFSQHTRETSNLDVLSRCKKLNELLSSDVLSGKGDAKEQLKEILGILHTGPKRPAFLDKPPVTAIAGKLGASTSTRIDRPVTSQAKCDPPRDGSETGEIRTEGDRRSGHPVPRSLQRPSEEVEDIIALLNGPPPPNTAQIAMICCTTEDATVSKQQRPDGDPQLKVNEAVAEQVRRPLAVIAEHECKAKKAAEAAERERKATEIAEAAERECKAKEAAAAAERGHKAREAAEAAERERKAREAAEAAERERKAREAAEAAERERKAREAVEAAERERKVREAAEAAERERKSVAEMERQRAAAETLRLQRVQSGAAVCIQAHWRGYCGRRQVAKVRQERAEQDGAARILQAMWRSRQAAIEHRQRLVAAGILQCSARAWRARRVFDQKRVEAFMRHHAARVIQHAFLAHRARGYTLGPLRVPPGPRPQSSLGVDGAALLAKQLRLRSAVDTRLAPERVCNCSGGDDDSARQHRTVMTHMERVRQVSANERNRRCDVGERAIMVGSEHRDTAEALAIARQGFGNCTPRVPSPSSPWSPALDCIQLDSRWTSSRSASEAGGLLIPGALSCDSAFALGSRPSTALRRVQGSLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIG1</accession>
    <name>A0ABQ5SIG1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ApaG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1470">
        <property type="entry name" value="ApaG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050718">
        <property type="entry name" value="ApaG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007474">
        <property type="entry name" value="ApaG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036767">
        <property type="entry name" value="ApaG_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47191">
        <property type="entry name" value="OS05G0170800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47191:SF2">
        <property type="entry name" value="OS05G0170800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04379">
        <property type="entry name" value="DUF525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110069">
        <property type="entry name" value="ApaG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51087">
        <property type="entry name" value="APAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApaG" evidence="2">
        <location>
            <begin position="189"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="261" mass="29333" checksum="6653596FE780F9D9" modified="2025-10-08" version="1" fragment="single">GKSFFKFTPLSNNTYELTTNLAVFLSEQIATTIHTNVHNRGSHFLMASTLYRCASNLHRSTAGTSGRPGVFRQVPSGNRGLQLLPRAADTEANASFSLSELVQRRKNIELEIKLAVKEEDFKAAARLKRELEDLNSNDPVMVAKKELELAIKDERYKDAARIKDQIKQLDQKLGAVFLGDAGLLTTHSTAVTQGIKVQVQSFFLPTKSSPSEGRYMFAYHIVITNETKETIVQLRNRHWVIVDGLGKTEEVRAQWKGSTRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIK8</accession>
    <name>A0ABQ5SIK8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12432">
        <property type="entry name" value="RRM_ACINU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034257">
        <property type="entry name" value="Acinus_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052793">
        <property type="entry name" value="EJC-associated_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46589">
        <property type="entry name" value="APOPTOTIC CHROMATIN CONDENSATION INDUCER IN THE NUCLEUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46589:SF1">
        <property type="entry name" value="APOPTOTIC CHROMATIN CONDENSATION INDUCER IN THE NUCLEUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="2">
        <location>
            <begin position="6"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="397"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="655" mass="66212" checksum="82FDECFB1B77C57F" modified="2025-10-08" version="1" fragment="single">MKASDVPALKVAELREALKELGLSAAGTKAVLAERLLAALAAAEENKGADSPPKKTTSDGSGGAPDAKDVPADSSEQPKPVGAADEALASRAVMEDVNKHAAESTPVANERKGRRASGKPAAAGTQESVDIHRPEKEPEAAQNLPGSSEGPAGSRAKRRSAHAAGNTAVASEVKLAKQASDQPASSGKGRDSATLKEAIEDTAITAVKPKASGSHEAEHEEQVDYGTDDIMAVDEAPVESKTSDGQAAPQEQLKENQDTEAPVDEMDVGTENQSEAGKRGETEVAADANAAATGSSHKAAGDTSLVSEGTPNPVAVKEMVTELSPSGGGKEAAEGAKDPGTQSGVAEAVKTAPSDSTLQIASSGRKRQPIEFKVDEVEQRVAAKRAARETTAGEGSGAQDKAAGTATAADKSKTQQASTKKAPLSVAASGAAATQTKVVKAPEPPAEAEAGATETSPRVIVPQAAPVEAAVNAGSAAGGRAVKLQKLDSGGRKAGLANTVTEAVTARLGAPAAAKKLAPEPLEGPATRALRIEGFVRPFTENQVRQFLSQHGVVEDMWMPTIKKYCYVIFAEVADGEAAAIATNGVVWPSTNATSILKPSYVSAEDAADAIANGRAGLADTPRGGSGVLLASPKGAIAGTGPQVGVGARRDTLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIT2</accession>
    <name>A0ABQ5SIT2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_013844</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11368">
        <property type="entry name" value="RNase_PH_RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033100">
        <property type="entry name" value="Rrp45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF14">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="45"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="10">
        <location>
            <begin position="196"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="328"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="338"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI69262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="364" mass="38640" checksum="6B72B32F46BE6524" modified="2025-10-08" version="1" fragment="single">MSNRDADTFISKNEMDFVLKALSEECRLDGRRPYDLRKPKFQFSLDDCSATVALGTTRVLSVVSANLETPFPDQPSEGVLRFHVDFSPMASPAFEPGRPGEEAIQVGRLIERGLRLSGAVDQEALCVIAGRKVWALRVDCRVLDHGGNVLDAAFLSALSALMAFRKPEVEVGGESGTEIMVHPPEVREPLPLTIHHLPVSVTFALFQGGDMLVVDPTVKEAAAASGSCTVVINPGREVCCVHKANGIGLTPEQFTRCVRLAADKAELLTKELKAALEQHEVARVQARVRRHQQVVGLPDPAARHVMILGAGGATAAGAVGATVAAGAAASGESSLADDNMDADDDIDDDDDNDTAEEADGVAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJS1</accession>
    <name>A0ABQ5SJS1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014274</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47692">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47692:SF2">
        <property type="entry name" value="ZINC FINGER RING-TYPE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="3"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="118"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="380" mass="41531" checksum="684D832BE2D46723" modified="2025-10-08" version="1">MDCPICYQPIALEDEAYVATCYHRFCHKCIAEWTVQQRRHPIHNISGGGRGNMPDYRCPMCKQPYLYILFDCVLNSFRVEFVDDEAQRRGCVPSGGTGMALSAGHRRRRALYVTQPRQTPQLPQQQQPEHPVRVHPLQGLGQGEVQDSGPGPCPKAQDLASQGAAGGTVQDPAATVTTRAAGKRTVAGCAAPAVKMRRHDDPELRVFVERELQALLLQEDVGMLLEHVLGVVKNISGSTGASSGNASRCAVASGSGISVSVLRNSRYVPPPAKRTRTMAPSLPPRQEFERVMSTEMESFLQQKGHMFASQLYMFLVSGLSVRGYDELVFGSDVAPQGMVDKKVSEGTERVGVGSGRQVIQDIVDLVDDEEYDDDDDLSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK04</accession>
    <name>A0ABQ5SK04_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="121" mass="11920" checksum="5F460A286E71D0C6" modified="2025-10-08" version="1">MAPVAVPPAVTLFSLPRMSSQPAASRTSSSWASMAVLNSGTNRDANAGPTAVRALSVPRSTPGGAAPPAADGGSSSDADTTVGAVATPPTCARAISPPWPVAPAFVADSTTRSSSWKKASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKC8</accession>
    <name>A0ABQ5SKC8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015321</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF25">
        <property type="entry name" value="MYB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="15"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="15"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="67"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="71"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="567"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="954" mass="92462" checksum="2C34E2D3F99B93CB" modified="2025-10-08" version="1">MASDEMDMDNEQWAGDDIKKGAWTPEEDALLTKLIATYGTKNWSIVAAGIKGRSGKSCRLRWHNQLNPDVKKEPFSEWEDGVIIMAHDVHGNKWAAIAKLLSGRTDNSVKNHWNATLKRKVSTNTLRNRFLRERHTLQWLLDHPELDASRQKEGKSEVSINGNWAGKATKRAQATLPASANTTGGGGGGLSRARRSGGASRRSRSIDSGTASDEPEEGEFDARDDGNDGVAAGPGQGSMNNVAAATLQRLTSNLAMNAAALGSGLGLGSGGLSSSAVGLGPAALGAGPSGFGPAGLGLGIGPLGVLGTGGGPSASGGPPGAGGAASGLDRANSAAGPPAVGGNRQLQVLDLLPPAAALAGLGAATAGGGNSAAAKYDSNGGGAATAVGDVSDGNLLGNPPVGLHGLGLGPLAGNSSNSSGVTAPAGAGVSFSISTNANHHPGTVSSNSGLPSLRPGIGSNHSGVGPFATSPADLELFNSLPEPTRLCLIELARLAGSAVLLERGHDRGPTGGSASISGLVARPGPGLGGRISFGSADDGGSSGGRMREREPGTITLPPPAQLQQNTPGFGELPGPLPLQSGLGRPLGPLGPRIGDLGLGAAFGGSGDGGGLGSSNGLGGLPPHLAAVVGGGGANAVPALKAQQLYDALGELNGSTSLQQLQQHVQNSVVRMAAGGGVGGNSTGALRLGGPGGGGVQHGPSAADGRHAMAGPAFSCGRRDGGGVISTSSLGQLGLPDSLGGLGGVNMGTDSAAAVLLRQLTRPAHGTGGAGASVSASSLGPLGGGGGIGGLNGLGGLPLGLGGRGPLDLPADLRSSNPVAGSAGVAAFGGPTGPGPDVDLESLRLLGSAAAAAAGGGGESLTAGRPASDLRMLQPRLVAGSGGGGTGAGPLGLGDLMGGSALGGRGSLAGLQVGALQQQAALQQLAADPTLLRGLSEELAHLLSAVDNAAAASSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKD9</accession>
    <name>A0ABQ5SKD9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014681</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.30">
        <property type="entry name" value="Peptidase M60, enhancin-like domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042279">
        <property type="entry name" value="Pep_M60_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031161">
        <property type="entry name" value="Peptidase_M60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051244">
        <property type="entry name" value="TCAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15730">
        <property type="entry name" value="EXPERIMENTAL AUTOIMMUNE PROSTATITIS ANTIGEN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15730:SF5">
        <property type="entry name" value="SI:CH211-210B2.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13402">
        <property type="entry name" value="Peptidase_M60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047008281" description="Peptidase M60 domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M60" evidence="3">
        <location>
            <begin position="127"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="455" mass="49366" checksum="C2A32E9E89D40E13" modified="2025-10-08" version="1">MFIIIILPWRTAWHALTHELLCCCCCCSLALRRAQSVFWWMPRSVEAGCTLDIQSWRTMSQACTMRFSTLTTCRRRGPGVPITSWATTTSGAKCSSPEPPRALYNLFTTYALEKTGVPLASWSNYGAVSPSGRAANRAAYFTAGAMWSTDWSVWVALDTYLQLKEGFGWDLYKDLYTTYQNFTYPLADDNVQTFIQTSSLIAGMNLVPFFRTWGFPITNRTVNITASLPEWLTDPMSPPRPPSVAAPPSPPAPPTLPPTFAPSPPGPSPPAIPPPPSPSPPEPPAVPITCRMWDRVVRDCYVSPGVANSTKCGACVAEMRFFGLNPALCIECSAYGNTSAAVQDVCLTECVPQAARGNSWACSSICANELNVGDDPDLARDCSTCVLSSKDPWSCGRCIEITQCLTDWRAARHLCFSCVASGMDPMSCAMCAGKATPVARTDCMACKCISGVSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKF6</accession>
    <name>A0ABQ5SKF6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_014611</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI69904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="126" mass="13964" checksum="199CA990C1FF7958" modified="2025-10-08" version="1">MASFSARLLNFWNSSTGPKTTHFWGPVANWGFVLAGLADMRKDPEYISPNMTGVMCVYSLLFMRFAWEVKPRNYLLLACHASNETVQLYQLSRWYSWSTSSAAKPAKVKHVDAAPAAAAPSVGKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKV0</accession>
    <name>A0ABQ5SKV0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquinone biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_015092</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Membrane-associated protein that warps the membrane surface to access and bind aromatic isoprenes with high specificity, including ubiquinone (CoQ) isoprene intermediates and presents them directly to Coq7, therefore facilitating the Coq7-mediated hydroxylase step. Participates in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the COQ9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.10">
        <property type="entry name" value="Tetracycline Repressor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013718">
        <property type="entry name" value="COQ9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012762">
        <property type="entry name" value="Ubiq_biosynth_COQ9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02396">
        <property type="entry name" value="diverge_rpsU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08511">
        <property type="entry name" value="COQ9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="COQ9 C-terminal" evidence="9">
        <location>
            <begin position="162"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI70246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="253" mass="27515" checksum="DAF949F8F33BB785" modified="2025-10-08" version="1">MNRVAPLGRGCLAASSWNFLGTLGRQNFSECGPSASSYRSVCSSSSGQSVKSLRKSLLDGALKHVAEHGWSDAALIAASRDLRLSPAIIGILPRGEGELVEHFIALCNTKCAKDLEDKQHELLTLKFNEGVAKALRIHLEMLRPVIDTWPQALAVAARPSNAPHSAKLLFSLVDDIWAVLGDRSTDVTWYGKRAVLAGVYASISLYMITDYSPEFRDTWEALQRRVDEGLTVDAFMEGAVHSNTSTTGTSTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLL4</accession>
    <name>A0ABQ5SLL4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_015217</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF2">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 4, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="14">
        <location>
            <begin position="184"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI70282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="448" mass="50539" checksum="E9A29CAD7E11CC3B" modified="2025-10-08" version="1">MGELVKGRVESSLPGLRGIVADDKDQEHALVTREGPTKGDAEIDQLYAEAEDKRLSKTSLLLDVLNISNVLTDGVSAMVDDSFNKCFTSTRPEPWNWNIYLFPIWVVGVVIRYLVLFPIRVTLLFIGFNTLIVVFLLVDLTLMPSKRKMAMQRKLVQWMCSAWVAAWHGVIRYHGPKPTPGKNRIWVSNHTSMIDYVVLCSYSPFAVIMQLHHGWIAFLQKRILSSLGCLWFNRTEVNDRAVVAARMKDHVNKEDAIPLLIFPEGTCVNNEYTVMFKRGAFDIGATVCPVAIKYNKIFVDAFWNSRREAFGKHLFRLMTSWALVCDVYFLEPQSIQPEESPQEFAGRVQAMIAKYANLRIVPWDGYLKYYNLGEKNPGLIEKRRRVLADVLRAYLGKTSAAPSAIALEKEGKAAVELQSDGKGQKGEVEGVALQGWRKLCVGGTVHPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLW3</accession>
    <name>A0ABQ5SLW3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="812" mass="85920" checksum="1B107E43A01C710C" modified="2025-10-08" version="1">MALDTPPLAEVIGLLGDPKHLQREKGLHGLTEILKSGLGAEEQISVEAGIKGLLAQQPWEKKLGGLMGAMAYVDLAQPGGLLLEQLRQAAMGLLEDGEVRVRMAVGDCLKSLAKRQGISVVEASFPEVLRSISRNFDRSEEPDDEAAEGPDGQRLRTAPMEDFATESPAQPGSPLERPSSPLMAAAAAEAEVEAVAAAAEGTFPAADGKLGGKVQEATAAAAAAENGGSSGAGEKGLASSRGAAGNGSGGSLVSMLLQSSYRKVRPGRGEMRHGTEGWKCLETSMKALQALVEGCGPDIGPLLDEPLRELLYKALHHPNRFVRETGHLTMCTVCAALRGPRLAAIAGQVAERLADGMGDNWSQVRYAACVSTRSLLEWLGEGEEREALMPVLLPPMCLNRYYVAEGVRLYAQETWLRVMGEGGRAAVARHIGPVVTHYTLQARANNHAVREAACACIAELMEKVDRTAVGPHVPALFRSLVVCFKDMSWPVRDAACIAAGRAVLAYPGEMRPLAGEMLGLWLAHLGENIPTVRANSAVALAKALRAYGDEVLPALLAALDDMLLRAREQAAESSKYSGLRNETQFGVAARKARDNDPQVHSDQEMFSCGSLMARFSTSYLIRSDGCMDHGFSRDKEPWEVSDGAVHLLREVAAVRPQAITDKHFEALAELGNLSTFQHAYNLHETVWGSLRTIGEAVGKPRFKRHLRQLLVPLLADCRCGHQLAEAAAQLCVEQLRAWVGPGIFAGYLPEGVDASLVGHHAAGQPGSGGGGFGGPLRRPPVLGGLLGAFATPAGTANTQGAAATAACAQGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNC6</accession>
    <name>A0ABQ5SNC6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="10966" checksum="4057A8D2BC50E133" modified="2025-10-08" version="1">MASARASSLLPTSLSTSALTAGHPGSAHQVQASLPRWHHCFVTLSGTSFGPKPKPGQAVRRDILEPPESPPPRLAHAASPPFPWPHRSFPEATILPTCCSSLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNJ1</accession>
    <name>A0ABQ5SNJ1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015962</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF26">
        <property type="entry name" value="OS04G0435100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="469"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI71048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="580" mass="62362" checksum="2DBABF931B08073E" modified="2025-10-08" version="1">MAGDVFMGSVGGCLGMGKFAILNANHARVDTSSGARCTSKNPVYTQGFTGSLHGSSHGSKHRKSYGPRPRAGKWDELKLWQMGYRQELLRSFTMLNSLALCTSVLSFFALSDTYGKYGLAYGGPISVVWGWVICSVFSLVVAFCLAELLSAFPTSGGIYYWSWVMAPVRHRTAICWMAGWLNLLGQVAFTAGLEYSLAEAIAGVVYMHTGGPSGMGVRLSKSALFGIVALMLLVHAILNSMSPSITAYVSIVSFFWHVVATLALCTVLLCTARHLNSVKYVLTTWASDRASHGIKNPVYCFLMGLLMSQWTIMGYDASIHIVEETIDGENAGSLALVGSVTMCSGLGFVLLLSLTFAMPSMYNVLSEDNATGGHGAILQLLWDVFKQRYDNGYGALGLAYVPLVGLFFCANASLCANARMAYAFSRDGAMPGARIWRQLNRGSHLPVKACWLMAVLALLLTLPCVYNDLFFATVSAGSVVALSLSYGIPICLRIFHNKHSFIPGPFNLGWAGRPMAVVACSWILLTSVVFTLPTTYPVTPGSANYTAALITAVLTIAAFLFYAPGFGGKVWFTGPAPNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNR5</accession>
    <name>A0ABQ5SNR5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">MLO-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_016288</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MLO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004326">
        <property type="entry name" value="Mlo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942:SF127">
        <property type="entry name" value="ION TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942">
        <property type="entry name" value="MLO-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03094">
        <property type="entry name" value="Mlo"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="274"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="2263"/>
            <end position="2283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="2295"/>
            <end position="2322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="470"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="606"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="805"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1074"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1157"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1255"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1302"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1481"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1605"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1662"/>
            <end position="1738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1800"/>
            <end position="1926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2068"/>
            <end position="2090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2110"/>
            <end position="2140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2569"/>
            <end position="2636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2650"/>
            <end position="2742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="540"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="564"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="606"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="626"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="648"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="674"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="813"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1100"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1314"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1605"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1623"/>
            <end position="1632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1880"/>
            <end position="1890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1891"/>
            <end position="1908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="2068"/>
            <end position="2077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="2115"/>
            <end position="2130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="2582"/>
            <end position="2598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="2619"/>
            <end position="2629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="2702"/>
            <end position="2713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2862" mass="290567" checksum="A605CF82A6F6BD09" modified="2025-10-08" version="1">MAAGPGVTGIEGWRVALLLVGFIIVTLSFEGLIDYVEKRLKKRKGLLTAFRALKNELLYLGFLSLLLSVTQEYLARICIPQTSGSTYKKYNLEDQCAEGKEPLWPVSVQHETHYFIFAVAVTHILYCALTIALTLHKVSTWRPWEDAALAESKSSSGDVKVMQETVSRSLIRSMCTSALAAMSGHRSALELAAHVKAMATSLAAQFRVSVSQPMYHNVRLLFIEKMGLTYDFDFHALVTNGMENQLAHAVHASWPLWMIATLFLVLPNHSHVPFWSYCLVMLLMLVVGAKLVSITALLGMQVAIKYGDSVLFGRMDMEPRALVRTGPARRRMSLSQLADALGDAVDSAATDGIFAADGGGGGDGGGTGSTAGGEGIGGSAAAAATGAHVLDEALPAGTGAEVQAQVAAITALTSREEALNLLRTMAAKPMLAEHPPTQPQEQRTTNEASNQTVQLNATTAPSVATTTAADGVGRDASAEASPVPAGAGGDLHGSEIPAAVSTPKLMPSPSLLLGIAPPRWGPRPAAAAPPAAPPPAPIHQHQHQHQHQHQHQHQHPPHKRKKSLPPPPVRLPVPTPMMPPIDIAPLSPILNSVAGNPMSVTPFSVTSTASPLTAPPSSDSPAGPLQDGGGGGDGGGDVAITVMGSDEAPAAAPSTAADACPSGDGASARNDRLAASVRPPVARPPTASLVRPISAARPNSAAGTVSPLSVLLPPPGGTQPPGTVVAPAFAATMRTGSRGLNAVVAAASWANTTARRNTSRPSAGLMLCGVATSRATTALEQMEEAMAIAATTVEMSAGQHPEPVISPAATSTAGGGGGGGSAGEIRPLTPSTPGGGVAGFLASLRSSRAAHLTDDSRALLDGSSSTETVDGGFHSPRVPVRQVSRLATTMTRIARRVASPAPTETGAGVGNAAGHDAAAVAAAGAHVMDPVVAAAIAAKAAATLICQMSPRRPIRRCMSARQLMCTTHIDEEDFSRTVLRRQPAAANNNATIANCGVCAPKGKAVVLRPGGVQDDSGIGLQVGVVAADLLPASKFGALPAAAAAAAAVPQPDLHPTLPHLPKFPKFSTAGLPISIFGPRKKPKNAHRQSGGSTEGSGDKASGGDGRGGDGGDCAAKVADLSATGEVAAAAAGSSSSAAQELLSVTGVAAEMSGSSMAESPAASAKDAAAEEASQPEPSAAASGATLGVSAPQLRGVLASAFALIQVKLQQTPAGSNDSAAYDSLSALLPPMSPDTTASMIPGFVRGTSNCDGADVALSTASDHPDPSSAAASINGTGASSGCTATNIQKTYMEPAASAHAVSSAGVRGGGDGGAAAASSAAQPPAAVTSSNSLPLLSSPEQSPSPDILRPRSGVRVVRVGDTGPWISVQEAVALAMGTSALESMESGVVATAAGSATAAEPLEYGNESEYGNGYGYDDALEAPAPQRGTGLLLSNTSGVQEATLLERPLPLRDGGVGVGVGAGGLPPRILASVFSMNTSASSGSIWDSPAPPPRPSQQDPAANEMETRLSTSVAAGGGVRTAASMGGSGELSGVEVDVEPCKACGDVLTDTVAGDPPSADGPARLLVAAAPAAAQAPSSRAEADLPSTPCAPAMPLTPHALETVEEGDEEAAEAEMAEAKSQACDKPRRGSHDAGASGTTEGKIFGKGLEGYRNVGLMLLPLSSSSSPRPPSAGEVEQGGDDPNSVSCSAREDSLGGGGSGTGVDATWRRESPEQPNLEDPSDGYPTGDPDQNLESTFGGFGVRELNRTAPMIGVITALRDLPTPPRIPTRTTGSLRLGTGMQASGSAVSLMSLAPWVGDEGFGPDPRSAGGGGSEFVSRAPSEAADGQRLSSASGGGSRPDSAAVLGEEPDAAGGGEVRGDSRLRINSRSLFGGAASQDVDDKEAEEEMPPPPPPPAAPPPAPPPPSHQRLHVLTTGGGRNGAAVSVADDSSRWAVAVPPSLRSGPILLGLAHSTAATIPINSATPARALPAGGHGGGDRIVVLEGHIGGICSWDDPLAGPVCEGSGTLDESSKGLVAAVTPPPLPPLPPPQPAADGATFVSPFGGAESLRTTAAGIAAGHIWRDRAKEHDEERRASGGGGGAAGPIWHGQNSHNSLLALLGAYHSRNSGRQANHYHNHHQQNHHHQNHLGHGSDSDSDDGGCCHTIWPPCIPKPWHHPRVAPLSAPVRDLKDNLAAGVDRAKVALTNAAGDVADTVKERFRYGSRPTSEQQQHRLLQRKISDHWLERVRNMLAAHKGFENMDASAFFWFHNPKVIAYVFNWAYYENSLSIAMLLFSLILGYQEKWVFKGVQIWAVLLLLVADLLILVHSCFYVLPLYALIMPVGSHCPKEVLRRALKAGVFTRQTALIAKALHLQLKARASVAAAAATSPAAPKTSFIERFAQRMRKSSMLPPAVTAATAATAFSRAAQTAMFPTTTGALSPGGGGGWNRAMSPEARQIQLLLSPGGVAGGVSGRINAAISPLGGKLSTAFGGGGGGGRLSGIEACASDSNGDGVGNVKGGEGGASRGSKGVGGIVSVDVGHLYNTSLKVIDLTDSGQLGDLRPTTLASVAYGGDVAKPALIPRKDAVAAVSPSGPRDSSGGGDGGGSAVGGGGSLALGIAPSSWPGREARDAPLTTEANSGCSGTDVSDHGQQAGATVECVTTAAAAAGNRPSPSQGIPVSSAAKGPLNHQRAGGAAGAVQQDPTPSRLSRMMQHFRTAPPPPPPAPADPPPRDGNNHHGLLGNAEDDVVPFTGSGAPPSPCDNMMPGLTRAAAAAAAPRAQPPPRLSIQGSINNPMGLMDLSVTRGADLERRGSGDSLSSSLGRSGTVMTLMSGMYRGAMAHLTPEERRDAAVRGLALGQAAGQRGRALLDMTLVPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPZ2</accession>
    <name>A0ABQ5SPZ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="2">
        <location>
            <begin position="188"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="723" mass="76061" checksum="81A580095CCBD770" modified="2025-10-08" version="1">MSGFIFNCYGRWRRKDGKHKEPQKLRLRSIQLPGCSHRPSLLLLVQLAVLTSTLLTGAIAAPAKKSPPPKVKLSPSPPKAPPPPPPAVAAAPSSGPIFIKGRLQYRTTKPGGKWILAAVNINVNYLLPKQPVDVTNGQPIPAGRYISLTCFLPNATATACSNITNAMVTQAAAPVPASNITLSVLVMLVTVTASAQCGNRSGANVTEVRNAFLGPNGYADFFGNCSYGRMVFDRQNFKVVSVAVNCSLAIATCNEDAISVAAKQQLPANIQIGSYSQFVFVLPNNMAVTCGWVGLAELPGMQSWFTPDSLGIFSKGTVMQELLHNFGLYHGWKDGVEYDDYSTAMGSGDSCPSAPELWRLGWATPLEQLNSTSFNLDIYKTFTLPATYLGPTGVMIKIQPDWLDPFYTKNLYLALRMKAAGDIDLLDQFAGKLSMHEVSRDIDNSFVAPGDPRSSILGALIPGSSVTLFNYKLHILIGDLDATTNSTAVTICRFLIGPNDCVDSTFPPPLSPSPWPPSPPPSQPPSAPPRPPPPSKRQLPSKPPPSPPPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPSVVVPEINEGGKPPPMSQKPPPKSSNSLPKSPEIKDAPSPRSPPKVEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ28</accession>
    <name>A0ABQ5SQ28_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="240" mass="26305" checksum="C93EE59593D742C2" modified="2025-10-08" version="1">MFLKVDERGVVIALVHVDDTCVASDDLELVREVKEAIGQCFKIRDLGEARVFLGMDIHHEKNGDILLSQSRYIEQLLERHNMLDAKDRASPLAMGTRATPVTDESELLEDPTPYRALVGELNYLAVSTRPDLAYALSVLSRSMSKPTRAAMSAAKGVLRYLAGTQEMGLRFPGGQGLQLDGYSDSDWAGDTATRRSTTGYVFRVNGTAISWSSQLQRTVAVSSVEAEYQALSSAVRGCQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQC3</accession>
    <name>A0ABQ5SQC3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_017222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000126">
        <property type="protein sequence ID" value="GLI71831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="296" mass="31863" checksum="D663D516A5E31BC4" modified="2025-10-08" version="1" fragment="single">MKKFSRKWIPTEKPLQRFGKLEGEESLTLQERLSARRLQIAEKGTLLKVLLSWGAPENLHQAGAQLEAQPVPAAQQEPQLESEENNVQQCAGGVMAPSGLAGAAAAIELAYQRLDETTEAEGFEGFQVQVSDLISQLEKLRSGAAMMEARKGGLSGASTDLQGSSITAKFAIIPAASCPAMGATSPPSAVEVADAGAASIFINKAAQQPQELYQPLELTQPPKPHPKTPERRLPPEARNWGLQDCRSWAGEQHWSSWAEVQAFTKTLEPTLAEAIREASLEHNPLVAFGKEQAAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RL81</accession>
    <name>A0ABQ5RL81_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000123</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00454">
        <property type="entry name" value="TrHb1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="196"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="416"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="471"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI58420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="487" mass="52334" checksum="D4076078D1C64D52" modified="2025-10-08" version="1">MGTSWSQSESWQALVTKEEVRDAVKGFEAWQKAREDLKSSMSVQRSAPSTGPLLERLGGPDAVRRMVEAFCRKLYGDNKLMVFLEHTDMTSLRAKQCNFLTWLFNPRDQPNTSRHVRTAHLRMIKQRGFARDHFELGLTYFEEAVREQGLSEALVKEVMAKIRPYKEITFTPLARDAEDEKRWALAERLDRPLSAMSSVTPSELRLKPAPHSSRPVTPPSSLPALRQSDKIQLLSPAQQHQRQQPSVFLSQQQLQQPVQPSHHLLLQPTDAVGGETARSSQPPSPSTNTVIHASTAATSPSSTASFPVPSVTSDSAAAVFATAKQLHGFSPEYESSLSVQSVNYSGTLIRSGAAHNTGAGLRGNGSEGVGDVGYSTTVGIASPLCPFTGARLSRPATSASELPTGVTEAARSVLPLVPPPPLRLVSSGPATPPGDRIGAPSSGPLSCPSFLPPLPPPPPLASARSSMPSSGNATPQRRSNMGYSNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN99</accession>
    <name>A0ABQ5RN99_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037688">
        <property type="entry name" value="ZBBX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28634">
        <property type="entry name" value="ZINC FINGER B-BOX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28634:SF1">
        <property type="entry name" value="ZINC FINGER B-BOX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="442" mass="44228" checksum="F2534ED5FAAB5E1F" modified="2025-10-08" version="1">MRKEATGRVKEMAPKVAKPAVLSAEAAAMEQKLAELRKAMEKERAKRDALMANTGGGSIWRNGGSNSGLRTSIKGKGPGEGSAASGSLSGGGPSSSRRPSGSDAPSRQGSGSSSTRAASARTAGTESEVPSVSGNRVASARAAMNVNPPEPKGRVGGGIIGRAATGGGDGGGSAAGGTSCGTDDNPALADGAFNEADSHKSFLEALNEWRRANRGDAAAEGEGQAAATTTSISGVSSVPQRLHSARPSATLEVQTDSRSASRPSSAKPLSYFDKLVINTSSRVAGHLASGGPPETIRTSLSGQATGLARSPSSSRPPLAPGPNTTIATGGAKAAAASVPVEMSSATTTATATVTTTTNSSPSAAVVSADGGGVASSDGRKAPLPDPLTILDRLEALERQRQAADDDDEHEEVAIQVTTATGLLLTKGTRLPDEVLMPPNQEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNJ4</accession>
    <name>A0ABQ5RNJ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000414</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052476">
        <property type="entry name" value="UBAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46738">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46738:SF1">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="231"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="298"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="703" mass="72008" checksum="3BB8A3BCB5DF5E4A" modified="2025-10-08" version="1">MDLVVRTLFKTLTFNGVDANSEVTDLKNLLHKRGELEHIPIPVASKQRLVFKGSVLTFGSLQNAGVRSGDHLVLLLHRPHPNTTPRPVDTIPVPTAATIRNAVISEARRRGIENTIVDEPARAFRPFSGMARDADQHLLQLVQALQGRAAATEGRSGQPRQAGGNGEVGSAAARPPAEAGAAGARRYLQPPAAFPGGLQVGVMPPGMMLFGMPASAAPATGPPPQPPPIPEPDAQAAAQLGEMGFGEAVVRKALLLHRNDMEAALNWLLQHGDDPAAAEPLTDEQLRQIYSRGPRGPPSEPELVEQLVAMGFDRNQSTDALRRFRNMDMALAFLLRAADEGGENQPHNGSVEGNAGAAAGMGAPQARGPEASGLPGADDQGSQPEAAVGPARLDAAASEGGSPSIHAQGGQEQEGRGSGGGGLSEMSELALLDGENDDVEMLLGRLTGRLAQAAGGDLHVHEGSAGRSQTGGAGQELSTDLSGLSPGMAVDSAGDAQRGAGDEGEDVDDDDDDEGDDDDDDDDEDEEEDEDEDDFAGDDGMPTMIEQLAMGAGGGRAAMLHMPLTGGGPRLVGTAILDDPMGGIRAFEGPFALYGSVPHGGTTPAIAVTRGPDGNELVLGSAGATMPPLMPARGMMMGGLGLGGGGGLGLVAQPLDLPGLTQASGAGEDELQGMGLVQQLLNAVLRDAMGGGEGAGSTTANRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP05</accession>
    <name>A0ABQ5RP05_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">adenylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000981</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02672">
        <property type="entry name" value="CP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="265" mass="29373" checksum="8DB0C2A2847606C7" modified="2025-10-08" version="1">MLAQQRVVTPRQQGTFRSGKACVRPARRSSVRTTATLKVMISGAPAAGKGTQCAKIIDKYNLVHISVGDILRDEVKNGTAAGKKAKDYMERGVLVPDEVVVEMVKSRLAQDDVKMQGWLLDGYPRSASQAEAIEKEGIRPDLFLLIQVPDELLVERVVGRRLDPVTGAIYHMKYKLPPPEVVDRLQQRSDDTEDKCRVRVATHNANVGAVVGYYKDVLREIDGTKSMDAVFEDISAALDEAITKKDPLEAYCKDQPAADECRVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP27</accession>
    <name>A0ABQ5RP27_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000889</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HscB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.20">
        <property type="entry name" value="HscB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004640">
        <property type="entry name" value="HscB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036386">
        <property type="entry name" value="HscB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009073">
        <property type="entry name" value="HscB_oligo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00714">
        <property type="entry name" value="hscB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021:SF15">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07743">
        <property type="entry name" value="HSCB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47144">
        <property type="entry name" value="HSC20 (HSCB), C-terminal oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="139"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="304" mass="34244" checksum="4C4A769366A69707" modified="2025-10-08" version="1">MTARVLTSFICRRRTQFSSLCDRVSPLAVGSLGDTCQPLTSAPAHEQAADQVIRLWTRRFGPNLSCSGWRSVHHDHQQCGDKNNLHCNERQHEGQGASRRHLTLEEMEKACWSCDKLVKRGGLVCNGCETIQPPDETLNYFELFSLPETSFDINPRLLERRYKQLHWNVHPDKMGQKPTEEREFSAQQASIINLGYSILKSPLSRANYLLALRGISAGDSFEGTLEDPELLVEVMEAREEVEDTDDPTALSALLVRNRQQQAGLVAELSAAFRADDMKAAVALTNQLQYVAKLEQEIIKKLPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP73</accession>
    <name>A0ABQ5RP73_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001187</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045112">
        <property type="entry name" value="PPAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661">
        <property type="entry name" value="PETER PAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661:SF5">
        <property type="entry name" value="SUPPRESSOR OF SWI4 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="25"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="470" mass="51718" checksum="469AA9CAA196C72F" modified="2025-10-08" version="1" fragment="single">MAKTRRRKKRTHVASDNNEGKKPDPKTLVFKRGKHGSILADLEQDVRRMMLPNTALNLKESRRNTLRDFVSVAGPLGVTHFVMMSATENSSYLKLSKTPRGPTVTMRVISYSLVRDVQAAATRPRVPVNAFKTAPLVVMSGFSGDETLRLVTTMFQGMFPALNVQRTKLSACQRVVLLSRDKESGIIRLRHYTIAVAPSGLRKSVKALLSNREVPDLGTFRDVSEFVTKSGYGSESEGEEAEASRVTLAQNMGRGNVAARQSRVRLHELGPRLDLELVKIEEGLCDGAVLFHAHVSKTHEEAAELASRQEQRQALKAQRKKQQEANVRRKAAEAARKAAAQAEQMRKEGKLPAEKMWWEQEPIEGPGSVSEDVEDEEGNGEDEDNANREGGAAKSVPKKRRRTDGDGDDEDDDAAYFREEVGRDPEADEMVGANKGGGGRRGRGRGRGRSGGRRGGRGAQADRQQRDGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPV5</accession>
    <name>A0ABQ5RPV5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001335</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46504">
        <property type="entry name" value="TRNASE Z TRZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46504:SF2">
        <property type="entry name" value="TRNASE Z TRZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="96"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="333" mass="36722" checksum="A1E9D8E05F2307EE" modified="2025-10-08" version="1">MIQSLARAFKEQKQSLTQHRQDATSSEASSTTTPTDVPVLNPPALLPDSRQNKITVCGMELEGISISGQETCIIFPRAKVTFDIGRCPQRACFQQTVLLSHTHLDHVGGLPFHVCTREMLSLPASRVIVPKGCGAGVRRLVDVARELQNSPPLEFDVLELQAGDDHILTSGYLCRCFPTTHTIASQGYLLYSQRRKLKAELLGKSQEEIRELRLAGEDVTDTISVPEIAFTGDTTAEFLDGPTSPTLEDALKARVLVMEMTFICDNVTVDEARGKGHMHIADFVAHAHRFQNEAIVLIHFSPRYKRTDIIAALNSMLPPSLRAKCVPLLNGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ09</accession>
    <name>A0ABQ5RQ09_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001428</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="340"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="423"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="530"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="482"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="497"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="573" mass="62785" checksum="4EB7149F90DF653E" modified="2025-10-08" version="1" fragment="single">MNRAARRFRTLLYALEQQQKAPLLNYLVEMLTRYNVLQHRQFCGICPAPTLAFSHVRPCPNIHHLLFSSSALSASAPSNPGCEPVAPISRETIGQVENAATTSINMIDINTSNAIVPPMHTGLVSLESDAATNTLELWYDLLRLLHEHGDFEDKETPRDLLIQQQDDVKDAIVNFARRRADILFNLPPLEVAAVAEAELLPRDLPDRKLRNADRRLKETYVTGRAAAGHGQLHATMQDLLRVLRDWAVVPPATVDTAAPALAPALRHLLSCIRHLASLEPDPVAMERAQRLGTPTAAELVERLATQPSAEDRRKAKVTAFLERRHRLRVRDAVGRGAVLEAGDWFCSNCGSANWKDRPRCFRCAAPSNKAGNSVVTPHDIAEVYGDRPPRRQPRTATAAADADALYDAQAAMAAEAADAADAASVGSGGKASIRGGADLRSPGSGRTTRNRKAPLQLPGDEEDDEGERQCRGGGRRQLAETAELRGRPGLRRRADSDDGDGPDLELDDDQGSPYAAEIRSSPIYQRTMREYYRDEDEEKVESSGTRGWRRVDPSGSRAHGRANSLVPGSDGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ38</accession>
    <name>A0ABQ5RQ38_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="166" mass="17760" checksum="DF5D8689B6038AE2" modified="2025-10-08" version="1" fragment="single">SEPAEESVSGGGYDDEDYASQVSTTDEDEEGDSYGDESDGDDSLSDEGDEGEVEHAGLLSGSASLDLTDEEELDFYYQPIGRQRRGPAAGRTDAGASSAEAAAAGQTSLGKGVEEGNEEEEEEELGARRARHEAVREWARTLVGPEEALQLEEALLEMYGRGEFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQM6</accession>
    <name>A0ABQ5RQM6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="685"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="815"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="696"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="708"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="765"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="782"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="820"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="834"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="852"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="868" mass="89845" checksum="57F1E3BAFC48CF67" modified="2025-10-08" version="1" fragment="single">MENELISGVKLNWADEVDELEKTREAEEAAKPSEPPRPATYGVLFGGVPDDFALWEVDRMMSDLLFSIQVIGRRRVPGEAVYELDLGSRKDVDTVCRRLNEIEIQGCRVWAHAKDGQSETYEEEYSYGPPARRSSGRLDTQYSHPGYDGGPYVGGPYAGGRGYGDRQPYGGGGQRDGPLSSHSSFSMGRSDSGPYSRTGFRDFSPTRGGHPPRRSGSREEDWHRGVPGPADRFGGGLPYGGGPRGGGPYGGIRGPFPHPQMESRYDDPYGPPGGPGRRPPPGAGPDRWQGPGGGDDRWGSRLGAPPPPLHTDLRDVMAPRPAAPMLPPPPPPLPQHQSMPVIEGGERPRLKLQPRSNSTIAVSASIGEDGFEAAAVDTSTAAAAGEAALSSPGQAVAAAAAAAGLSPSSAAAAGKPKSNPFGAARPVDTLSREREIEERIARERVGVDFKERAFVSHSSAPLLPPPQAAPGAIALPAVAGMPPGAGGLAAGALVPGPLAAALPPGAPIPLVGMPPPPPMPHLIAIDLGAAAGPGGAASAFERLPPGHPHLHSHLLELAHPAQLMPGALPGVAPATAAPAPPPPQMAQAAILPMAAVAVPVVLHHHPPPPPPPPPGPMQRLLPHPPPLPQQQIPPPGLMQPPPPPPPQMQQPPPPPHLVQQQQMQPPPLGMPYTIDLHQQVQILQRPRPVESAVAVAPPPPPPPPPAPALSATMSPPPATVTAPAAAPPPHASAPERPPQHQRSVAPAATSAPGPEGGANVPTEEAALRRAAAEQETARRQASEAAAHAAAAAEASAAAAAAVKAVTAMAALTIQAPPTPSEATESQQRVLTVGDGDELDEEYGEDEEEFLDAQEHLHSSEDEQHGTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQW6</accession>
    <name>A0ABQ5RQW6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="608"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="608"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="702" mass="70060" checksum="70263247509085FF" modified="2025-10-08" version="1" fragment="single">MDPTAISEDIYAIAAGLVWTLSLSLNGFKLWTSRASSKSSWTTDGGNATVSTVGQTTARPHSPSDDIYGEIYRRRSPGPSGPSISAPGLKALLVCVDSSRSKAFEALCATEDGNSPTAAKTPAEHWASSLPIGVVGTNANYVDVGRGNAQGAPETAIDGDKAPGTAADWPTNTADHADGVDVMTASAAAVLAAVSGSSNRLNSNKDVDVDADTAAITFTPSSATDSASSGSSGSSGTDDDALAAQHLHVIRGHLVKSPGSGDMWLPLGGQIAPGVPGTICSPSAAAPAAPADPGTAALVAAAAFTLAAAVAQNRSFGGAVASVSPPAVHGFDTCRVRCNVSFVAREGEDGGMGVEGCMPECSQCEDDDEETSEEGDEEEEEEGQGGEASDVSTGGVSVVLSASQPQFRSHSLCEGIYTLRSLSLSPSAASPGTPRRFQSSNPTSERVGGGGSNGGGEEVVGPVLEPGPEQEDEVSERRLPAFSPSSPCGSLMCGEQFLLPTGATSTSAYPSDRSHVELPSQGTHGLPCIAKVAGTGESASRCGGGNCADQPDCRSNSNSSSSMDQLLGGVFGGEAVASAVAATISSSGSAGGGSDGDWPGCGNCSGSISSGSNSNTSEGGSEGDRLSVRSCCGSAEHDTLSLQLDSSEGGSLISTGGSCVQACDVAVDVGKEDLPAEHYSRGDAVSLTLAKEANSSEEAEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQZ2</accession>
    <name>A0ABQ5RQZ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyrosine-protein kinase ephrin type A/B receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46104:SF1">
        <property type="entry name" value="GENE 9195-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46104">
        <property type="entry name" value="GENE 9195-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047482887" description="Tyrosine-protein kinase ephrin type A/B receptor-like domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein kinase ephrin type A/B receptor-like" evidence="3">
        <location>
            <begin position="86"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein kinase ephrin type A/B receptor-like" evidence="3">
        <location>
            <begin position="138"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein kinase ephrin type A/B receptor-like" evidence="3">
        <location>
            <begin position="316"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein kinase ephrin type A/B receptor-like" evidence="3">
        <location>
            <begin position="587"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="722"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="850" mass="88806" checksum="D73374D966BCE3F1" modified="2025-10-08" version="1">MRPSPTFVVLVALATVATVYAQQANGPTVIPQKKALPAGFRITCYDGYELIFTNDNYTNGVLASPDAYKYPIGQCRKCPAGTATMDGFRCIPCPSGYYSDDGARECTACPAGTVPRSTAPASGTTYYQVQKLNKGPEANACQPCPPGYFQPNLAGTVCLPCPSGFVSTSGATSCTPCNEGTFHGDGRELTGSGVYTTWSMKNSADTTTGFEATTTDINKVDGIAVSSGLPDYIAIPNTCIKCPTNTYQPLKAQAATSTIGAAALSACRRCEDGWYSAPGSSSCLPCPAGSYRNSYFDGTAALTDPGSLTYNDAAEYAANTYSACFKCPKGTYAPFPGSSVCQPCPAGTNAVSTGATECTRCPAGTNSLYGTRAQQMNWDTSANAYKSYTFAFFDNADKQYKMLRTGTDANFWLAAKAEACQDNLPGYFTDVAGLGIQLPCRPGTFMDPAATDKTTCLTCAVGTFTEDFTQPICKACWPGSFASQRGMTYCEITQPGFYTNPNTVAVNATVDLTVFTTLEASGLVKGAQDPSPCGLGYYQPEYEKNTCLACGSGTYADVTGLPVCKKCQAGRFQDSTGQSTCKQCDTGYFSNYGANSCTACPMGSITPKPGTSRCAKCAPGFYADKSVAATSCKACPRGYFGPYIGAYSADGLLPEGPRGCFKCPYDTYADRPGMTVCTNCPNLDLGGGTIVPQCTETPGSQRCKPCSLLVITNATRISNFTSPPPPKPSPPPPLPPSPLPPSPSPPSPLPPSPSPPSPLPPSPSPPSPLPPSPPPPSPKPPSPSPPSPLPPSPFPSPPPPGSPALANGTGVNAPGDRTGRRLLRQTAASAEEIAEERELLKLAEDKEDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRM1</accession>
    <name>A0ABQ5RRM1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052578">
        <property type="entry name" value="PI_Transfer_CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF6">
        <property type="entry name" value="F16L1.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="75"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="299" mass="34018" checksum="0B95F442546616E2" modified="2025-10-08" version="1">MTVSDLTDHEQHLVKQLKAEVAPIVAQHACLKQFCNEHTYVRYLRARGWNFHKASRMLKATLEWRLEYKPHLIKWEEVKSESLSGKLWVYHVQDKAGRPIVFMRPRNQNTKETDKQIRHLIYTLEVASRLADKSGAGKFTWLLDFNGYSMANAPPLKVSLHCNSILANHYPERLGLACCYHAPMLFSMTWKAVQPFIDPVTKEKIVFVDKGGLENAQMAARFDLDQLEQCMGGNMPGCAYDQDKFTDKMHVHDKAVAEELEQLRLQMAAEAAVGDHHVAAENAIQGTMEGAEQLKVTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS64</accession>
    <name>A0ABQ5RS64_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002553</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF5">
        <property type="entry name" value="AMINOTRANSFERASE CLASS I_CLASSII DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="46"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="460" mass="49454" checksum="2181FC46D4C0A0E8" modified="2025-10-08" version="1">MIPRAVAETGSAPSNKSKLLSRRIDATDTPVIDFTRRMMLKRKDTLSLAQGIVHWSPPPTALERLAARMASEPASMSSYSPNEGLPVLRAALRHKLAVRNHLPHYDVIVTPGCNQAFLNALLSLCDAEDRVVLFRPYYFDHLMTMQMTGSADRVVLGECDPETMRPSLEWLRKVLEGPMPPKMVVIVNPCNPTGVLLTRAELDAISTACAAAGTWLVIDNTYEDFVFAHGGEHYCPAGRHVVHLFSMSKAYGMMGWRIGYVAFPDYEHHNKDVEGELAEALTGPIEDGTVATNGGNGSTGQPASSAPGSAPGALGLAQLKVQDAVCICASQPGQLLALEVLTEDGREYVEGHISNLAANRDLLRGALAPLEPHVVGGEGAIYLWARLPSGCGDDHAVVEWLVQQAGVCVIPGSACGMPGYIRCSYANLPPEKFPAAVARLRAGLEHLAAHGMTDMQQPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSU2</accession>
    <name>A0ABQ5RSU2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_002877</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Na(+)(in) + H(+)(out) = Na(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF197">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="464"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="112"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="661"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="809"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="917"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="995"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1236"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1291"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1392"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1933"/>
            <end position="1967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2120"/>
            <end position="2155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2201"/>
            <end position="2231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="678"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="749"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="809"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="827"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="948"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="995"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1251"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1316"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1935"/>
            <end position="1959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="2201"/>
            <end position="2218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="2219"/>
            <end position="2231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI60675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2231" mass="235051" checksum="5109A0F9EEDF6550" modified="2025-10-08" version="1">MDIIMNSNQWMRSLLSGGAPSAGSEFCLGKDVSYAHRIDNPEGWNWCLVPDGGYDAVFFAGLAIAVMCLASPRWDALVVLFFGGIYFTFAYYWNLENVSNAVMLWLGMRPADIFVFVFLPPFLLDLSVRIDFFMLKKVAFNIAFMAFVMVAASALLLIPFMLYALDLKSSGWTAAYVALFGSMIASTDAASVSAVLRSGGGPEILSVLLEGESLFNDASSLTLFEIFKEVVLEDARTTLANDLQGIVVKTIWSAFTGICIGLAMGIFTKWVFAWLQHRNMEAMAEVAWSVAGAYLSFYITQVWCKGSGCIGAVAYGLYGSATLLWGMSTKARLSGIFTQFWAVMTFIINGLIFFYVGASSVNFTIRSAQSLYTDSGGIPLVKILLYKLPLIFLASFALRFVLIFFSFKLLQLVRLSEGLSIQEVIFVTVGGLRGSLSLVLAQSAATLASTHDQNHTNRRVKAEIAIYTAGYVIMTLLINAPICGPLLTLLGLDKIRQEQLAMRGEVKNLFRKFTMTAIEDLAAGQDDDELLQGLDWAMLKLHTSFDKQLNKELPEIQPRVRWLSCGFWLSAVAAPLKLWHYLVACFRGRCNPDVVELHESEERLSKQRQDAELKRVTNINLRDLNRGESRVPGAFSMPFAPSPVRLPTALVIRDPIGVTSSETATTGGRGLTGGPNYSAASGASRRPPATVTTNALRRSASHRLSSLVVGPPGAAVGADREVSVHGGRGGFNRSSSRFLEGAVAGPTASRKSPSQLQPPNSPACGSSQEMQVAKSNDTSEETACGGENGAIVEDPQIEVLLSAPLPSADTAQQQEKPQANPEHQQCGGEGGAGGEGGDLFDPQLLALASTREIRHEASSDAPVVDAVPADGAPAGISVVAAATSSQLPAKISPHVSGGDTLIDAAVLVAGNSVTAGKDGAGPELQPQRGSGPLGPASSLPGSWARASAPSSPKLSNALSRPSPLSRGPTGGASSGNIPSPVGISRLHVSTSLSKLNSLDQQSQRQPSEVDAGAGATSEPEMTPPRTSGEGGLLQSVLVIKPGAMVRGPALPSSTRGQGASVRLVQRLGDECGRGSALEALRTDAIAAGARGLGRGSVRLAPAAMSSVEFSQPPASESEATLQASLAPFAATDRPLARTSASAPTAGRPLGRSSGVFPPAIGDGNMRSVGSEPPILSPFEFALPEDIASGAFPPEPFRAVGARGGAGDTAPLSRGSVRLGALDGGLGRSSVHAGGALDGGGGLSRPSVRQGAPGGGGGGGGGGALARASVRLGRSGGLAAVSSHLARIYDADSTSAVPSTDLRPSLANLEPPPPRASVTTDGRQQSGSTSPVCAMVGQNPTSAASHIALQVQNLQHRDSQQLLASQSQSAAQYSLGASGKHVLMSLIRRSVGQMRSNSERGGSGGNSTRDLSGRGPELARRTRFADSNTATALARASASGTLTQMNSEGEAGGSVGMCDPLLVEYRLRLLTGLKQYYKQKHAAGSLSPSAYKVLSYIADQSAHHPEQALSLWTMAKREISSGSLMEVEVAVYFRLKQWTAAAKASSATLVRTVVPRMLVPFVRMISSHTSATTLRGLETAVELWASLTQSLQTEWLQYSGAYGERLLEEVQHEADGAWAYIIERRIEAPKKFGAVQTHRAVIDVLTQQQSFVREMNEGGMIEESECRKIVEIVEKRLQLLARRGPRWRQPTVMEILMGTAVLRGVSPRVVEWIRKESTFRVYPQGAAIWDMGRKQAGAEPDGLIIVIRGVVRMMLQQDGALVPYYLGAGGVGGLISCVLESHVPGMSSAAAYAEGNALGKGPLVMHVPWPVLQVLKRFSRELRMAPYQALDLQMYRTAAVYVLDLLRQQVTQQFSAFIRSSLLTILSSQRLDGPTVRRQRIRLLLQPAASDHIVDGAIDWIGELLNTDLEIARQLLDPAAGSIPVGGANAGNHYYNHLHSHNHSHSHSHSHSHSHSHSHSHSPGSAHHFASAGLTAVQSDQQPQQNLARLAASPWVEVMYKAIAAATGAVVDIIRSSLKDSILVVLGPKQGIVQRSTMVLIQGSLEPAPITHADGKGGSAGMGGPFVASSNEFHMAPSVMVWVSDYFEGELVAQKELRVVAGPRGATLLLWGVHPEDLLLPSPTAESLGGAQPPSPGGGGTGGDTPGKASGGRSVAAVGKGDQDAMWGGGFLSVAKSIAIKTRQESSKLKPDAVAAAGAKGVAGGAANGGQVGGGCVEGKGGEPKSPKAAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT27</accession>
    <name>A0ABQ5RT27_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_002235</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051953">
        <property type="entry name" value="Plant_SW-associated_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997">
        <property type="entry name" value="MYB DOMAIN PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997:SF75">
        <property type="entry name" value="MYB DOMAIN PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="50"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="54"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="137"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="382"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="807"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="860"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="909"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="997"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="812"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="829"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="926"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1097" mass="115471" checksum="0262973B843197AE" modified="2025-10-08" version="1">MDVAQSHASEKDYERRENVDFGVRWLQDASDPADIVRGVHRNVSTSALDARSSRRLHWSQQEEMALVLRHCQLGNRWSAIARYLPGRTDNDIKNLWHSTLRAKTCRRSSLLYCYVQLVRDCPDDPAARRSAYDKAMQMSHTGVNGTSTQGTGSGGGGSAGASPRMAPYRRGSIPGSCGGLSPGPSPLRASYNGGGLVLEQGCYQPLSDISGQMIWGSIDSPAGAGGGMIAGTAGFTTGSGTTALGLASPQARQYHLAAEPQSQQLPATHAPLVMRMSDILSPPRLQQLQFSSVLSRESPQRHRQQQQMEAPDQYHGVSFSQSSFTRQQQDPFLQPPPQLFPQQQFHSQIHHQQQHQQPTSMLAAVSDLPGRTAAQIHRARFGGTFGSSHLPRSGSDSGVVGGESQRGLSTSFGNMGGGAGMGGVQQLISMHGRCNVLSTGQHYQEYQQNRPSLDAVLQQQQQEQQQQQEALFQQRSAPLVQLQLQLHHQQGQHQKDDGDLADSFFCRSHLRGPLAPLPSAGTCPSRVPASLSLPPSPIAGTSHGGFFASQLQLQPPQQHQSPQLASMTLQPMQPPLRQRHLSAPGIQPQVLVQPMHRLSSSSMAAPAPDVGVSATMETAAAATTSTAAAATSVFDSDAALAAYSTALMSTAADSLDLTMYDLAWLPSATAARQSNANTHTVTANANISAMMHDSLDGPFTVPMPPAPQLPLSRSTDVQAEEYFSMGAPQRAQARALSTPSTSLLFVRSATAGTVGAAVTTPGVGLGVAGCGGDDQVSEMAGSGSGLSALLRGISGSVSELRGDFQALHRGGSQTRPPSQQRDQQRHQQQHSLLPPAPSSAGAAAAAGALLRAGLRQQQFMPPLPSLSRDTQQVTEHPNPLQHQQQQEQHEELLMMESKDSLDELLARFDPVQPNHGPMGPYRDPPTDQSSWPEQLNPSSTSHASGSLTNALGIGCNSVTSDAPVLAAVPALEPTCGGLAPPGSQQIVPLGGLKTVAGPGQGAPGATSEGSPSGHVPAPTMSSCCSSSGNGNGVWGLFNGGSGGNGTASTVSATAVRTTVLGLQSRSLAPQNPVAAPTTDAAVTMGLCDEDFEAFWAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU02</accession>
    <name>A0ABQ5RU02_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002818</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009563">
        <property type="entry name" value="SSSCA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051888">
        <property type="entry name" value="UPF0148_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16537:SF1">
        <property type="entry name" value="PROTEIN ZNRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16537">
        <property type="entry name" value="SJOEGREN SYNDROME/SCLERODERMA AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06677">
        <property type="entry name" value="Auto_anti-p27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="393"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="635" mass="67862" checksum="54103645BBD97A8D" modified="2025-10-08" version="1">MSFGVKADTSKRLGALLLRGWAMLGENCPECQVPLMKERTSHERLCVNCDTTFVPDGQGLRPTNRPLQKLADADLQQQQHQSQENEGEEEGEVEGEQDGHGLAGDEEDGVCDYDPRADPRLGPFASPAPECQEDAEIKKSHGQSFGQTKLVECPGNRKGTLDVGATAGVPPGPASQSVTTTSGIQDRSGQGQNKPLATYGLGRVGAPSAFMATSGAPVGKPSSGAVPSAEGPEDIADLLAAKMLQGWALLDKYCPRCNTVLVRSKANKRMFCVACDAWVVPETDALQQQQQQQEQQQQQKGEEEVREKEQPQAEPPQAEPAPAVQLAIGSISAQPQPQPHPQTQPEPPPQSLLPPLSQQQHRQTQPEPPPQSLLPPLPQQQYRQTQPEPPPQSLLRPLPQQQHRQTQPEPLPQSQQVTFLQPSSPDPRVFAALRGLPAFVSAPAHLPSGAIPSGGAPYISADFQQPQQTYSQLQQSQPQPPLTYQQFFLPVAPVTTMSAGSATFSLGSSPPQQRSGGGSSFTHEVLLSALRSESQQQPQVRPPLQGSSFHAGQAQGTGGNATGLGAGGNSGNGGNKQPSSTTLLLSIGRTVALKMTEAQGLLERTPALDTERARSYIALISECLELLSKLHSLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUM4</accession>
    <name>A0ABQ5RUM4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAC3/GANP/THP3 conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003411</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF3">
        <property type="entry name" value="GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="3">
        <location>
            <begin position="432"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="861"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="984"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1100"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="894"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="996"/>
            <end position="1025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1907" mass="196856" checksum="443D331177370BC9" modified="2025-10-08" version="1">MFGGGAGAGNFGASTQQGQAPSGGFGGRGGGFGGATGRGFGGGNAGGAFTGRMLFGAPLPSAGQVSAPAPEAGSGNVGAPREPSGGAAGGTAAPANGGQQQQQRGNAVMPGRGVFARFGIAPLREGENSDRGGGVGRGVTGHAASGHAAGGGGGGRGRGGGPYGVAPGGRQGFGGSGSGWGGRGTGRGGSRTRQQQQQHGGRKMVWVAGTQTSAGGGGDMGVEEEYDGDGDGAADDGGTTEFDGNVGADLYNEGPEDAGGGGGDGEPAATAGQPGDWRSGRAVAAAIGQSQPGAAGRAVRVPVPLFNDKRVAGGDGTGDAAKLKQRQRRFGQTSEVAGAPAAGGIKPGTGFGAGALVLSGVAGGDATPPRVASRSPARVQADTEDIIGHSPSPSPPPSPLLAYLSGGGDGMGDDDDGVAGGGAALVGTCEVMCPVAERKRREAAGELNIFERLDPHNSKLTNENLIIKKARKNYSEEDRRPENLRTFRALGLTMARLRSLIAAPDETALAATIQCSPEQTLLNVQAFLWDRYREVRKEIIAQHFHTRAEMLPQILAWNEEIARFLIISSHELWGNRDFAAQLNQEQLKKVLTDLVTRFYTSAARLGVPTPNSAEIKCYLLILMMGGTIEKNGRRFRQPAEAQMYLRQYTQKELESPWTDVLFAVMATLQSGNAVAFFDLMSRAPYTLACICASHVMPMRSLAMHMMAAAMGGPHSDQPGGRRNPDAAIPLPDLARMLKLSEANTSTYAETRQALVQPGGATGQRDVSFLSSHVTSREPRAKPQHWISSKRARSGRSQDTVNAAELSPELLSFCRLVLAGSSPSPVSHHLPIGQLGAVAGGLRSPVQGAATAAMPLQQTPLTVATGGARPPLPAAALPSPSPWEYSPEAQGSGGSKAAAAAAAATTGGKGPYGSTSGPAREARGSGFSPAKAANFVFGSPTFSAGAPATTGAAAAAVTSPSFHGFGTASTASATPAAVAAPRGVTRGFGLDNATDAPQPFSQQPMQQRQQQQQQLLPLQQQQQQQQHAQSGFAQTIQPRPNMVDAGLLAQAQRAAEVERQARENLRHQADQAIRQEHERNRALMVQFEQVAASASASQAALLEAQQRAASYAREVQSLRLEKQKEEIEKQWRGITVRRCVGKWREAAQQCKRRQEVLRQQLARVSVTFNGAWARSPLNMLRRDCNGTSGREELEAKVAAEQSPTGRKRRYDLLALNGGALTSAQAAAPTDATVFRARNGTLTPHLNLGDDILPGLCRAIANSQGTGAAASGSGRPTLGSRTVRYCSFRGAAGAAKHGFRRVEEWELGNRKWLDPALQRHVFWKMLLVSGAQEMDAHCVSGFQLEAAAWIRARLSCGRTAGPRPNRRPEVGEQLTIEAARVVVSDQACNLTMCVQDIRAKSLPSGGPSASVGSVLGFNSRAASVASDASAVATAMAATSGVMMLVDGRMLETALADGSSPQRAAVKAAQDLNESWERLRKVLSNLPSTRSTKQSQFLPQGPAQLPVVILTLGSSASTAAVGNHLAAAAATAWPPAVASRLLASPPVTGSTAALGGHVKVLSLADAPDEALRLSLSHLTISAPLYDHVVTLDLEEMARGAVAEVVSELDERGTAAATATVAATIAAYNHLLTVYEAAVMETAASPSARWGLPAPEVSVSDFDAAPACWAPERISAVVLELRRLQLPPPSPEQSKLPGGSAVLPQLLGRVATWSVTTAGPRKVMMLAALAPSLRRQLRLRVSVPPLPLPLLLPAPAGTAAHETPAWRPGEAAVAPHGAGHGLAPEPPAAGAFLSSAPVGVTAADNGAGASIKGALITPGGPAPSVTPLGYVALMADVMATPTGAPFADGPAGAGWESSNPTISAVRSPPYGFGIERSVGRGNEDAVAEVPVSETKRMATEALKALLAALGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUP9</accession>
    <name>A0ABQ5RUP9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4-hydroxybenzoate polyprenyltransferase, mitochondrial</fullName>
            <shortName evidence="8">4-HB polyprenyltransferase</shortName>
            <ecNumber evidence="8">2.5.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Para-hydroxybenzoate--polyprenyltransferase</fullName>
            <shortName evidence="8">PHB:PPT</shortName>
            <shortName evidence="8">PHB:polyprenyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003276</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an all-trans-polyprenyl diphosphate + 4-hydroxybenzoate = a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:44504"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:9564"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="2.5.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
            <orientation evidence="8">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01635">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006370">
        <property type="entry name" value="HB_polyprenyltransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01474">
        <property type="entry name" value="ubiA_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="380" mass="40739" checksum="9E4926A4195C648D" modified="2025-10-08" version="1">MSVHHMATAACVQRLRIKSGTELMDSLLKLLHMGVHEQGTRAPSGDDPPSTSGRGGSPSLNLSLVLHRRMSTKASSGSETYKSSNWVMRNLPARLVPYAQLMRLDKPIGTWLLALPCFWSISLATVPGHLPDPVMMALFGAGAVLLRGAGCTINDLWDRDIDSRVERTRGRPLAAGTLTPHDAIGLLALQLSLGLLILLQLNDYSKILGASSLLLVGTYPLMKRITYWPQAFLGLTINWGAMMGYAATAGACDWSIVLPLYGAGVAWTLVYDTIYAHQDKRDDVTAGVRSTALLFADNSKKWFTGFGALMAVCLTTAGMAAGAGTPYYAGVGAMMAHLAWQVRTVDLSNGPDCMAKFTSNKWAGTFLLAGIVLDRLLGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUT9</accession>
    <name>A0ABQ5RUT9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="243" mass="24298" checksum="B0E8C2F9F2F4A820" modified="2025-10-08" version="1" fragment="single">PPSPPTLTPPSPPLAMAPPKPKPARLPPGPPSPPSPRPPKAPKPPPPPAPAPPPVPPRPPPPPPSPPSPLPPAPPSPVPPSPAPAPPPSPPEMGPGFPSPSPNPSRPDPPSPDPPSPDPPSPDPPSPEPPSPPPPSPPPPSPPPPSPPPPSPQPPSPPPPNPPPPSPPPPPPPDLPPPSPPPPSPPPPNPPPPSPLPPRPPPPSPRPPRPPPPIPRPPPPRKLGWKRTASPTSSAGGYEEGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUU9</accession>
    <name>A0ABQ5RUU9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_003709</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="204"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="177"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="556"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="756"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="828"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="984"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1172"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="179"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="588"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="771"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="842"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="873"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="912"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="987"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1004"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1027"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1051"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1101"/>
            <end position="1135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1200" mass="126565" checksum="551B4DC032A30C0D" modified="2025-10-08" version="1">MPVASHEVTRNPASSGRGQPLPAAQGPSPAQPQPADMVFSDRAAELVLLHQLGGQLVNSLGLPQGLIRLRRPEAFQVYLAARQERGQRPDEQLANEEDQEKEQQQQQQQRRQWRRRGRREELPEQQELPESLGELPDATLPSTSDAELTEAAGLMPPAMSGSEAAALLEFRRRQTRLGAGKDHSGHDTMKDGPADYGENTPRSSNQRCNCKKAQCLKLYCVCFTAGLYCGNCACRECRECLNTPENEDEVLAERSRKLAASPGAFAPKVEVTAEGETVCHKKGCRCRRSFCIKKYCECFDAKVHCGLSCKCEGCQNMPSGGNAGPEAKVTTAHYERSDINGATAATDDGTDVHAPEPRDAGQQQQAECVGAVAAGMPQLAQGPLSFAMAPGAGPFLLSLHPGYPGLAGLAFLENHRASVGMLGGGGGRDDGPMDGSGPIEQRLDGAPSMRSGKAISADGAVPSLQGFTTDLLEAKPHHRASTATRMFAEADAVLDSEDQAAPVDAPAGPRPDGPSRTPEAAATAGTCGAVTCAAADAATECAGPVTSAVVATVEPAPEVAPPPDPPAGSSAADGSGAGEGCPHQDPRAATSGNAAGAAEGQRDKGARVPAAHQDSPMKKSDIDDGAAANELSRMEDVLAYPMLLLDDPLRAEELAAQVGLDKPQQLAARTAVCVLKALLNMISSAVQLRRSNTQEDVSSGPVTAMPLASHDMLGRNGPRAGVQPAAAAASAAAAAAAAAAAAIAAAGRSYLYVPQRRDNQRPWRQQDTAGEGEGAQGGSGMRQAIAASAVGRGPCASGDTAGDAICDGGGDSTGIMPLAHGSYRFRATVGQSSPDKSPVSMSKHERNPHPRGADDATVKKAAAGGSQPPRSGARYDGKEVARQLSDDPEASCTQPTPGGGTQGHEVGDAANRGRHGGQRPLRRGSRVKRPNSLLADMELEEYPVIHRQPRLHIQTQRLCTMEPPSPKRARLFAPVRGRDGAAAVRSRAQHPGHCGRRGGWCGSEEGEEENGAPEEQSESYDAEDDELAGKGRMDRQPYARTHSAAVLQQPRPYQTSHNQHLNRLRAGSATGDRSAGPRPRQFRPVPSLRSRVVSHRRPLTVEDEDEGEGSDEYGDEDEEEEKEGEEQEEMEDEEEVAGKLCGARNVRTMTREMLAMQALFDLRSANAVLPVPRGAPHRQMSPVRARSKTTPLGAPRVPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV58</accession>
    <name>A0ABQ5RV58_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine desulfurase</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_003380</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02006">
        <property type="entry name" value="IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010611">
        <property type="entry name" value="PRK14012.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="10">
        <location>
            <begin position="61"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI61099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="460" mass="50404" checksum="CD3506C6FC80B7D5" modified="2025-10-08" version="1">MLQRIGFQILQHGRMLPAVLSQTSAALRRFSAVPQPVEDAETGSVPIIRKKGISLRGVPLYLDMQATTPMDPRVIDAMLPYMTEQFGNPHSRTHLYGWESEEAVEDARAKIARLIGADPKEIIFTSGATESNNTAIKGVATYLKDKKKHIVTTQTEHKCVLDSCRWLQQRGWDVTYLPVQPDGLLDLNVLEAAIRPDTALVSVMAVNNEIGVIQPLKEIGAICRSKGVYFHTDAAQAVGKIPLDVNAAKVDLLSLSGHKIYGPKGVGALYVRRRPRVRLEPLMSGGGQERGLRSGTVPTPLVVGLGAACEVTQREMEGDLRHVRRLEERLRTGITSQLQGVVLNGPADPAHRYPGNVNLSFACVEGESLIMGLKEIAVSSGSACTSASLEPSYVLRALGVEEELAHTSIRYGLGRFTTDEEVDRAIVMTVTHVNKLREMSPLWEMVQDGVDLKSIQWAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV85</accession>
    <name>A0ABQ5RV85_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003809</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1031"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="803"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="805"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="1058"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1347" mass="141214" checksum="199231929A2817C2" modified="2025-10-08" version="1">MPCSWLWATDTLFVCACGAPLAWTIRYCVRDAAASRQRPTAEYARTYLARRVLLQHARGLRAAQLCAGIFYAAIMSYVYLIVSPDKLTSAQIVGLLLSYLYVATTCWCTAIGLVPYLEKTLLEFNPVVSIVSVGSLALFTHSTGTTGLATYNGFLSKVLAYDVLWVMEDCPLKAYLPLHLVLALMHATGDLCNGSQWPLPYMALGYAGLGLILPCIVSGLLLPANSQAKVPGALHTALAAAVVGYLLLDQLLRAAPLVEPLQDLAASGRSLLARGVVSLLDAPLEGTKYSYGLPESTGLYVTWSTLVVLLMSALLAVISEGDSTLVSGTASGTLLGSLSKNSAASALLAMLRGLSTPKKYAVLAVVAISLFQGVGIISIAAGLVMHGPETFVRSAPPVLLAFMVGVVLQVVGVLYVLVLGSSTDSGSALTATVAGIASVETTAPATVVVSDTETISRSTSVGGVTTPLMMGKSMLTRANVMAQETLLDSSGGLGGGGPAANGAVDAYSVLSGLSRGGSERTSFQDNADENAHDLRTRIEHLANFRTFMQGLDQQPGVYSLEDFIRGAATAMRGIFPGVAHASFCLLNSTRNCVNMYTFLGDGCRRPPRINVAVAPGTLIYHVLHNCQPVFINNVAAFPGPYADVQFMRFVLNLRSVACLPLVVAGSRVLGVLRLGFEDWWNWTEHEKSIAKQLALVLSSTSTGMGVNVSGMQPVAQASYDFRDEGIAGVDSDADAAVNMLHGVESGVPSMASFSRASDGAGLAFARTQPLGATLNPQVAQYQAYGDGISGVGGVSAPLRTYGSNSGSGGAGRSGGSNAAGLTRRIRGPSRSALQYAGTSPTAGPGLGAGLALGGQPYPAAPGSLGGFDSGAQLGTNFTANAVPAGMHSHRSSMDYHGISSGGAGGGYFGGFSQGYPNAVGSMGDGGNVPGAFGQTGPGMATAGYVIGSNDGSGGVSSGAGGQYGLRNFSNLSCAGIQVGQLMQSSSGSGQHLGGEIDESWLLQQPGSSQVRRALAGQAQAQGGGGGGGADLKMLEVIGRGGFGSVYRGFWRGMTVAVKVLEHEDELLPCTGSSGSQSKVDTSSRRAALLEGAMTSTISHPHVVQTYDYRVVHLDPQSSLNGITRSRVLHETQIIMEFCDRGSLQDAIEQGVFVVDGPRGARGGTNDPEVDMELIIMTLAEVSAAMEYLHKHRITHRDLKPKNILLKTSNKDRRGYVSKVSDFGLSQVLPDTNKTQVSTKYSGTVTHMAPELIEDGKVYQQGDVYAFGIIMWEIYTSSTPYQSMAHPQIMVGVVTHNLRPKFPTNCPEWYKSLANRCWRKDPKTRPPFTEVTKSLLAMLPDKSWSATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVA9</accession>
    <name>A0ABQ5RVA9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sister chromatid cohesion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003892</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23958">
        <property type="entry name" value="SCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026003">
        <property type="entry name" value="Cohesin_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12765">
        <property type="entry name" value="Cohesin_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="8">
        <location>
            <begin position="1382"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1355"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1574"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1770"/>
            <end position="1857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1355"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1574"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1796"/>
            <end position="1816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1827"/>
            <end position="1837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1857" mass="200032" checksum="1B66A00D3A5B76EF" modified="2025-10-08" version="1">MAGPCFKLDLLSFDDPAILLPLPISAKICCVSLQQDGSDKLDEQMSQNLQLLEQLDTVLTGTDISYVRPVNLDVVNIPIPSSSLAKSLVNRNPSLFKYGVAVVEEDAPEAAVTTHGNPSVMAISAHNSQLDPPGASGRHGEGAVSGPDNLELGTTSGKFSVKKRARRAVREEVYVMRSDEAKAAAAKLSSFLESSIAAATGHVTASGSDDEDGQTRVGNAKGRGDLGNRQQLCSIVHLEVLKDLHAQLLTAQTQGYLHLVPHKLLRKLLLMLHGIAEAGLNQLLLDDTELKSPHLARVTTALEAMLCELVIYATPKLPPSLNMEKLLNCAVELTNYHLQSNVLALHDARFKGVYRQGGTEDEDDLVKSAKVLRARSTGGSTAGRGSSRWTAILAGQLEQALSLLAKVMSLIRMDVDRLFPLMRISQQTLAVASLRALHYRALGLVVSMFKYYPLHRETVMGKVTEELLSHPSTGKHVSRCFPLQALDSMTNIQMSSALVMQLIQVAAELPKVDTSHLDALVPAKMHMHWSDLFWHAVVAKLPSTRAAKTDMSADLKAWVEALVEDLLVALPLPEWPSAAVLLRRLICIVRKDRMASDAGMQLVWIELLGLVARGLYSLARDAEAPEAVRAVIAAVDAVIVAAGGEITENRLDKNEVAQELLLEHLSSNGGSSAGHDANNSADAWGLDASVAGSAKQLIACDMLLAHAKAVERQQGHKLNDKQTNDIVMCYRQIYERQRSCLGVSISGLCDLDRNTAVVLSRWLVAKSVLGCSRVTLLSWLAAAGGSGRTQKEAYAAAARAKSIKLLGGAIEVDMRVLYMPEVQVGIKGALNDDSILVREAALDLIGKHICRSADVAAQFYDVLASAAIDAGLTVRKRAVRLLWECCVRCPEFERRIEAMLLIVSRVTDTEDTMRSLINKICAEVWFLPNFQLESEEGSSNVSRGAEQRAANLGELVMAIYCRMGSAISIPVHSTTPIVVIIRNIVGEEGKSEYEAVRRGAREVVDVLLARSLELQGEQDAQPQPTTSGRPEGHGPAEELFRCLLALHVLCVADVTLLLRPEDPQRAIRCLAPYLKEMPPGPTDNASRRKAERLLAVLAIVGGCMTSLRHMDDKLADEIVQDLRSIVCRVSHVQAVTVACQCLCAMASLKPQHRNTVRMEASTCYNFLDNDLKKEVTAMPEKQRLYPRLLYTLGTLCRYGADILDNAPEGLKHPTCAAAMDLVLRFYKLLEDNIRAKDTALQALGFIFIARPQLAVNVHDVGPVLEAALDRTAPASIKARCLSSLTDLLRSEEDVLLARQAAARKEAELAMQATTLPDAENRALARRNGEGDSSSVSSGIIQMLWEKILALATDTTPAPASTTCGQTPPPTPSGTAAAHGAVVRRRALELIEVVIRGGIVVPWEALPALCALATDPERDTANRALAGLRAVVAANTAFVAGQVPRGVTEAYNFHCKLAVLERPGQSPAPDKCRPLVEGLSGVWTTIFQPDKALKAKFLTALLKPLDEGCSLASGTAAKSDPYLLSFMTYIIAELPYRKMDELLAVLVRINEIVSRRAEEVLARFQELREAARPAKAAAASGTHTSSSVPTASSTISSGNAPSGLPGVVKASVALSLLLLLKQYLRLSYEVTAERIAKYEPTGQQRKVEEKFAAAHNGKLRFNASKLRVDVAARASGFVAGPGTSAMAATAAAGLTAVAGTGAAGLVSVLEPGVEEVYKVLKTLMKQDEHDYKQGVESLAADAEAVGTSPGQKDDLRDVAINLEELMAVPEAAGCGAKKPPLASRRRGRGLRGRGGLESTSRGQPWLSSISGASTSTKRPSKRKRICSADEDSDDDDDGKSDSDLVVEVPHKARPAKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVH2</accession>
    <name>A0ABQ5RVH2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="512" mass="55568" checksum="D70ACD898C64FCCA" modified="2025-10-08" version="1" fragment="single">FAVEALIMCKEFFASLTLRPYRTTTIAPPTVFAFVHSFKYQGSSFLLAAKGAPCLAALCKAVQLNPTEPSNGRFESTRVNSKGGKYNGKSQAGNQRDVLTRTDSSGRLRGTLTQRPHQEPSQQKVPRHNVPAQRPNTGPAAAKQHSKDSTDRFGSLGKWLARVREEIKLADKQRTPAAAMVKKEGSAHVANPEMAAVEAVPMLLPQQPDDELRALGSRPLPNLGGATPRFDPKMLPEVVRVFHRASMRLRFTTQLPQAVATYVATASQRGALDLATYDVTFCKDLLLYLGHAQVVNRECLEEVRKALQNRWSQLSPEDLVQVLAALGRLNDTDRIASVGRSGLGASRLLRMLVDAEPCVIPLLEDVGSSNAKPGDAGDSAAAGLPTVGALQLALVYLSFYRSATSGHQHFATAIMLQHNLEMCLSRVDPVDSRQAPADELGRLVAGMAGVTLLRSHGLALQLPRFVSVIMKRAAELSQPVRALLLDLPPQHWAIPPNWKKTLQSTASAGRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVU1</accession>
    <name>A0ABQ5RVU1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF36">
        <property type="entry name" value="DYNEIN HEAVY CHAIN, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="713" mass="77345" checksum="E7704949F4EBEB36" modified="2025-10-08" version="1" fragment="single">VANLPREILHVVVVSAAPLVTYPEVRNLLGEISSKGIPVPSAMLATRGTGEGGGNVASRAAGSFNAEVLRGAVDVRSMMTRLQLSILNDYNTAMHHPAMMPYRGHEVLWGMVSRFTTEAYRSSGMTPEQLHADLTATSTWLSEMTNARASVIVGLVTLEMSALQRAALPVIQKAHSGIMLLLGGLLVNQSLSLSSRLAEYTNSMVARPSQLPEFVQYVEVIWPHLDPMSTTRQSISADLDHMTRLHVIYIESGAGGANGRASTPTLYHPLYWAASQNLDDDDELADPGKPPSGRKVRATWENLQAVVARYLDEARAAADYFQAAAVLMVTHLKTSLIESGNALQILLMQLRTGLSTDASADAREVSTHLDSLAEGLRTLAASAEQHSSWARLLTHPSTTSLVGSLESQLATARGVLTARCALWDALAGWRLFLDKLYEVPCTDEERARLAAELQEQVGRIRMHASKITSLHTDDDLTSTRTVTPAEQKLGAHLSLLVKSWQDILPMAQQLVHPAVKPRHLRMLLGWLSAHQTAAAAGTSGASGGGLFNPELGSPLRMMQNPYAALPTHVRQDDAEVAAAAGRTSFAHLVTACSESAATRALVMRISQVALGEAQLVSWLRSIQERMQAIPLTYSQVRTWNSFTPTNLPEVLASLVALVAELGGVQRSPFYCGVLREYERVEQLLGGVQRSLQDAAACLDKWTYLNQLLHNPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW38</accession>
    <name>A0ABQ5RW38_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CSC1/OSCA1-like 7TM region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_004347</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1455"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1519"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1560"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1722"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1748"/>
            <end position="1766"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="23"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="1230"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="1480"/>
            <end position="1739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="213"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="562"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="778"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="929"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1143"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="580"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="794"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="935"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1001"/>
            <end position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI61840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1829" mass="193839" checksum="4CDC28AC4D62B462" modified="2025-10-08" version="1">MTVNISKSHTAFEFPYVVSDNQFKTSAILNLILGGVSLLVFIALRGTLRSVGGVYQRRRELADLFLQAPPLLLCGLHQAWSWVTTVMCMSDTAIVRTAGFDALLLNRTILMGLQIFSVLTLLALGILLPTYYSFDDVVTHRLHRGTNVAELSRTTISNLPPGHAILWLPTVATYLVTAYCCWVLLVHCQSYAELRMAYMLCLDAAGAEQYQEKREVRPVRRPHEGSVGLGPGRLCDGNPSGSFQERNGMIRSDSSRPTNSFAAAPAATSPVASARGSLVSLHYLLPVEATMAAQPQVPGSASLGGPDLGGQATGDCSSPARSHLARMTGLHNHELQGLTPDGLRSNLSNGSLQAAAAAGLQVAVSVGPAAHRASGLVLFPYDTTSAAATVGTASAMEAGRPAATPAPHVPRSMSLRVRLRLVAPWLRHVVVRAAERAARHKAADNLLAWVNPLKMLAKDREDWARQQLAADADPDPSLPPLPSGCPPPATAAYAGLLVASEDMLMALTGRGEEVTEPHSRSPCFRRAPRVHWSESVRELAKSLGILLWPDCGLNAESSLGEKVSQARSRANSSLRGAAGRQEETGRGEHATCSHPVALGSSGEHHKGSAFTAKPMVGAKAAVTTAQKVFHGGGGGGTMGIQSHGTGMAGQTGQDAANLRKAVGVGTGGSLRGYGSKSHQAPPVYRYWAPAAEQRCFCRDLRRRQRRQVPWQQPPGTPLGFDTVSNPVFELSTAPTATCSAAGSISGGGGCKVTAAVGMNQSIGTALLRSRVQGAPAVVLNEGSLDGGGATPAEVGEEEEEEDDDDDEDDDEDEFEAGETGRPWTGWQQQPLVVGRCNARLRQLMKVRHGGRSHLVNCHHYAVLVRDVVLRPMERFNAIGLPVVRSAVEECDPLCTGDVELGRAIGGGAGSISATVGAALSVVNPQSVSVKHARRCSSSGGSSSSDYDGNDRRGGRRRARRYGPEIGSTVLGTLSSDENGSGSEGGEDSKSEHNPLLTGNDGSDGGEGSGGGLARAPQHHRLLLQPTSDLPIRDRAARMIHELRNHRKRNRHRRTLPIPTPRDGDGGVAAETACAAMRPSDGHVGARTVAVELSPACARGVRDADGGCSGEADDGPNRCDNGTPLLSVTTSPYRISRRANSAIVSLQAGPPSPRERAGREAGQGAEEPRCPSHEPVSGPGVASCGVASAIGLDDLSGVGSNGRHGVPTAVAAAPERSSCIGSVPAPLPLMEVPYDDSEEAAAVAAYYTQLFPDSFQRLVPVCNHRAVDELLFQWTTAMGNLQAALLAAHVAVRSTAMASRASTVAVAAASLSSSPSSPAASAATRLRSVAAAAALAALTRAPAMPASSGQPLAAPPDLPPPGSAHVAAIRRAAGAVRQLQDRIVIERARALRNPTRRAYIALFSSQRDAAAAAQCSPLAPPSRAQPLHFRACAAPPPDSVYWPALWTLPTGRAARLLASLPLLAVVVFPIGVLTGALANLPVAVCGGSAELNALYWPWFCNRQQSHSMYARLAKSLFTGLLPALISLGWNAWVLPMVLYLLSAIQSRCVSLPAMDRQMSRWFFWWSLLNMFLGAVVGGGIFQQLGAYLQNPGMLLLRIGTALPTTSNFFMHYTLTKGLYSNWLRVAWPHLGSMAGAILRSLAGAGLPRSWQDAFLIHSPPGYRFSSFYNGIFQTLMVGLAYVVTAPLIAPIALLFFLTAYITWRYAIVYIYERQYESGGQMFPVVFSHMTGYLLVAELFTGAVLLTNGAWIQATLLWCSITPGLVAFHRLCVRRYLGPLEHSPLSLAMASPPASVDPAVYMPPALREGAVGWYPESGKVWEQYGIPKYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWC4</accession>
    <name>A0ABQ5RWC4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="262"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="334" mass="36145" checksum="785CFD9E5B62C44B" modified="2025-10-08" version="1" fragment="single">GLRPSNQPMVSDGTLLGTMTLQKDLKPFSSSTRLPYQTTNGGPGVESSTGSNYFLMAPVDISMADAYNYNVPADVRTGEDAVCLFSSSAAKSGLIIYCNLNPCCPGGEYNPYDLLVVDRARVDPRHHYVLTQSGVTQMLGGNAQELQPFHAWQRDRNLFTVLRKLVFFKHNEVFQAFTQWRDAVRVLKFRARQRFLTQQLLVLSPTFHRALLNVRRMTLAVSCDTNGTWRKGPEDRWIELEPELVAPSIMPPQRSSPATAMSSHQPSTSSSQQPPLLPPRQASLPRLAQNSSSGSGPAAEASAVGAASSSRGQSHSGVSGAAPGSAPALLGCEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWJ8</accession>
    <name>A0ABQ5RWJ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004566</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10098">
        <property type="entry name" value="RAPSYN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10098:SF108">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="7">
        <location>
            <begin position="156"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="626" mass="63593" checksum="AFC19A721BE93877" modified="2025-10-08" version="1">MVSGSSSPWLYKAAAIASTVALAGGAGYIIYKYVLAAETEAEPEPAVSMQELLTGGAAKAAPRSSRATGIPVRRTPSPPKPRAGPSLPTRKSAANKVTSTAAAPEEISVHAVPSTEDSTASAAAATTSTTEPAATAATATAAAAPSKPATTGLKACACCSQPLKSGGLPQRCSQCKSVYYCSIACQKRHWPEHKPNCTKAVSKLEDASATAQVPSPEAADDTTAAETATAADASGTPAIAGAIDASSRGGGSSASAGGLQGALVEVLRQAAEKGAGTLDGAFEEAVMHFLGGNPSSALSAFQGLLVAAKEQNREEMVIEAQKWLGHTNHKLGNFNAAADAFKAAIEAAKAAGNTAAQVDSAVGLGNLLKVAGQASQAAEVLKEALMIAQEANSAGMQSDVLVALGNVVMAADPEEGLACLQIAVKLREDEVAKASETGDRAGMATGMMQAAAAMVNMAAGLFATKRYEQAKQAYEQAMEIFELMEDHDKVIQVLINLANLAELQLDRPGEALEYRSKLAAALKDASHPGIAGGAATSPCGVCKDHISIYKARSRGDEHGPMLLLGCLHVHHDDCFKEWCDKARGAAAVVAAAAARGGEEAEAAVASPGAKRAAVCPTCQAPVPVMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXK5</accession>
    <name>A0ABQ5RXK5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004728</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="5">
        <location>
            <begin position="486"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="211"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="941" mass="105238" checksum="C4CF6A19E377E1ED" modified="2025-10-08" version="1">MSDSLRLNMARGTIKKYILPEDLLQFAGNTTARDLWVALAKKHIPNVRNFRMQIQQQLKDFEMLSGESIDDYFRRFYKIGYKASLISMKIEPEDALLHLIRGLSSDYDGVKSVLRHMESIESVDQLQDMLKTEEGELRLQRNKAHEVSLMSLSRTHDGARVGPSQGAGAGNADGGRSRKACLYCNKEGHFIAECRKKANDEQRGIWRPALKGLRQKPQNPPALAAPPPAPPPVAPAAQPPAAAQAAAPPRPAQGQRRQLIARVYSSQQDDWERELWLFDTGAEVHVTGDKDKLRNYQHCGSDCKVWLKTANGSVVRPEGHGRVMFRSSCFETDVEVDVYHAPGIMGHILSLKALEGSFGPNVVPQFSGVLPHVEIGGAPAIELEKINGLFYAEDSSYNSWMGRYGSDRCLSGKKVREELRKVKLAITKQDSNLGVLWHKRYGHLGFDALAKAASSDMVTGFGASVDDVVAAKGQFCKPCAEGKQTRQPHPSTGNVTSKRLDMVHMDLCGPLPVKSWNNAKYWVTFYDDCTKFSEVRCITDKTVVPETVKEVIKLWERQTGDKVRRIHTDRGLEYVNEELASTLRDMGIFHEKLIQMGFEVSNANPSLYIRRDLDGQICTYLLVYVDDVLIASHDEQTVLEVKEEIKQLYECRDMGEAEVFWGLVIKRDRETHTLTVSQEHMIHELIERYGMVNADPRSIPMSPGTILMRAADGEVLDRSRYPYIELVGSLMYITNCTRPDIAQAVGVLTRHMSAPTEQHWRAAKAVVRFLLATPQCGIVFGGERVEEGLIGYTDSDYAGCIESRKSTGGYVFTLHGGSVSWSSRLQRTVTSSTMEAEYMAASEATKEALWLRLLLSELGYSLRPTTINCDSQSAIKITKNPVISGKSKHIAVRYHMVREQVARGAVVMEDCRSDDMADILTKPLPLEKFAKHQKSMGVCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYK6</accession>
    <name>A0ABQ5RYK6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12381:SF56">
        <property type="entry name" value="B30.2_SPRY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12381">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN U FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="518"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="564"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="741" mass="80152" checksum="2453255F4FE11E58" modified="2025-10-08" version="1" fragment="single">MDTEGGASPFLYSVCLNPLDCNLDAVLSIDRLSTHTLSERGCGYCWSGVRGNLGLFARGKYFFRVTVGPIRPVNVYNTTALTTASAIRVGVSRFQTAVEHLGEVPFSWAFCSNGSRVSLPAASTDAASADGSKDRGVGGIQYPAFEAGYGPKLSVGDTVTIALDLTSPKPMQDGRAEVPTAGRGPSGMAVSGNAAGPSNPSLAAPIAGTGAAGGSGYSSDSGSGEGDGTVWMAVNGAPLVRLFSVPLPNHPTESYFPHILLRNISATADFVGPQAPEAQQQLRPQCHQPLPPDLLKQGFRPWQEALFLTADCVRTMSSGPLPSTSECEVIVLTGLPGSGKTTWARRHCEQHPARRYCVLGMQLVLEQMRLACPRTRRLDYVGEPEELYALLADTLTHIVKQAPTVPRNYILDRTHVTHNSRRAAVAPFRAAGFRCVSMVVVVSEADLHKYQKLAFENEGKMVPDEAVARLRAEFTLPHPHEGFHDVKYAMLGVIETFTTVNRQRKEAQAWMAWKFKTGSGAASGSGPPQQQQEQEQQEQQTQEAQQTQTQTQTQEVQEAQQTQQTQTQTQRQPQTQQQMQTATKAQAQPRKGRCPRKLSSRPKPSLPHQPPMPPLRQPKPPPPIPPPVLPVYSIHQSQQQVTLEQLLPPQRQQQNSFQQQHQLQSQVGGSMLASGLGGPSSTVVFTQPSSVSGPVMCNPHGSMQPSMLGFISGHMPAMDMPHVPTSMHWIGLQSGDMGLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYT9</accession>
    <name>A0ABQ5RYT9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HhH-GPD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_005366</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359">
        <property type="entry name" value="A/G-SPECIFIC ADENINE GLYCOSYLASE/ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359:SF18">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="10">
        <location>
            <begin position="161"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="54"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="330"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="57"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="425"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI62690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="495" mass="52565" checksum="1B09F48CF1DF7906" modified="2025-10-08" version="1">MCEATFAHNLAHLYSKSCIYYSHIKIHLNHFTKPTMPRGAKRAHPDPAAALEGAQGPLQQQPVNSPPCTATSPVGTPTFSPSTQPPNSTLPESTLAPAPTPTPSKKKPKSLPAAPSFQPAIADQLRAKALKIQQQLSVLYPSPPIPLQHGSAFQLLVAVMLSAQSTDAKVNIVTPELFGKGPDAAAMAAMEVSEIERIIRVLGLAPTKARNIQRMSQILVEQYGGQVPESFAALEDLPGVGHKTASVVMSQAFGHAAFPVDTHIHRLAQRWRLSNGKSVEQTEHDLKALLPELTWRDMHLQMIYFGREHCPAQRHDTRGCPICSWAAAPTGQSGDRDGNGGSGSGSSPSKIGVMGAALQGKAIRGRAGRSTQQYLTADPESGHCPLQDVKLNGSKSAAKRSNRPSAAPRETRAKYPVDTAAGKAPTTTTVPPANCTHSAPAAVTPGHSGDDFAKSRGPFDVFRAGGHVGNSLPARPKGMITRQASRRLGILGRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZA3</accession>
    <name>A0ABQ5RZA3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase 61 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_005011</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049625">
        <property type="entry name" value="Glyco_transf_61_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007657">
        <property type="entry name" value="Glycosyltransferase_61"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961:SF124">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04577">
        <property type="entry name" value="Glyco_transf_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046106209" description="Glycosyltransferase 61 catalytic domain-containing protein" evidence="4">
        <location>
            <begin position="30"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 61 catalytic" evidence="5">
        <location>
            <begin position="313"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="615" mass="69995" checksum="53DBC68CD664FD13" modified="2025-10-08" version="1" fragment="single">MCCYEMGLGCRLLAVAIVLRLLGPHAVVAQALNEVANITGTSELSTQTEQPSDNDEPEAPRIHFKFRGREWHPESFGHTKCSKPIPKINPAGNIFDCLILRNVCLDQGVIIYQDSRYHPWTSEARLPHFNITDIIWNTPSVLGIGDKWKSGKNRYQFPLMRPWHHMEESEDVRRPVFSNCTTPLLFFHHFPFNVAEVYRFAVNNIYFMQQRLQFFDDHITLVPGSPPMSAVPSFTRFWLQPFSRYAVTSLGALSQRWPAGSPSAPSASTGEGEAVRCFSRFILCKLTLKKPTGAFFDAGQFVAEYYAEKAQHLTTNYTQRLTAEVPLDDQQDDTVLKVVFAVRSKSHKDIGRVLTNEDELIERCNRQELNFPTDPTVSIFRRVRCFRHIFGVDNLYDMWLVRQADVLVGVHGSALTNAMFMRQGSSLIELRPYGFSGRESWPNIYMKSQTRGMEVFWFGIDVMNETLSSPGDFEADMQPIYTRAKGCLARDRNLAVPWEAMAHQLLNVAVVSRDIWRYKVLRYSHSYYITHDIKPLELRGKDKRVPMCLQTWFTDPDDERRRAAAIPLTPEGAQKAAAAEEALLAKALLEEEARANAAVHAAVQDPDQVPQNVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZZ9</accession>
    <name>A0ABQ5RZZ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rubredoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00350">
        <property type="entry name" value="rubredoxin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048574">
        <property type="entry name" value="RUBY_RBDX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48136">
        <property type="entry name" value="RUBREDOXIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48136:SF1">
        <property type="entry name" value="RUBREDOXIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21349">
        <property type="entry name" value="RUBY_RBDX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="1">
        <location>
            <begin position="44"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="142" mass="15561" checksum="85248AEAA9ADBF46" modified="2025-10-08" version="1">MAAMQTTSFLGNRAAFKSSVRGSVSRQSRVSVNAVFTKNKVSSKVAYVCLDCGYLYDEPTPFEQVKSYSCPVCGAPKRRFKELRGNSLRNNDPKSMVARKEKLREQIAAEGGNPDEGQNEFLIATSAAIVVTLGLLAYLTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0P0</accession>
    <name>A0ABQ5S0P0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-dehydroquinate synthase</fullName>
            <ecNumber evidence="4">4.2.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006110</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08195">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00110">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050071">
        <property type="entry name" value="Dehydroquinate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016037">
        <property type="entry name" value="DHQ_synth_AroB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01357">
        <property type="entry name" value="aroB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43622">
        <property type="entry name" value="3-DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43622:SF7">
        <property type="entry name" value="3-DEHYDROQUINATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DHQS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-dehydroquinate synthase N-terminal" evidence="9">
        <location>
            <begin position="172"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="3-dehydroquinate synthase C-terminal" evidence="10">
        <location>
            <begin position="286"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="474" mass="51428" checksum="48E799BC698FBAF5" modified="2025-10-08" version="1">MSHTSLYFKSYVKSGSYSARGAVKASRVHAVPDAAPAHGALSSAAPSSSTSACTSNYSRRISTVCRGVNRRMVQWLRERQGGNSAGSHRFALQAVAAPERASVSMKTVNVDLGDRSYPIYIGTGLLDHGELLRKHIPGKRVLIVTNETIAPIYLERCRKALLEGGQLQVDDVILPDGEEYKSIDVLEKVWDKALECRMDRGTTFLALGGGVVGDMTGFAAACYQRGVHFVQIPTTVMAQVDSSVGGKTGVNHPLGKNMIGAFYQPRVVLVDTDTLATLPDRELASGISEVIKYGLIRDAPFFEWMEANMERLLDRDPEAIAYAVERSCINKAEVVAADERESSEGVRATLNLGHTFGHAIETGTGYGVWLHGEAVAAGTVMAADLSYRLGWIDKTLYDRIVALLARARLPILPPPSMTADQFRSLMAVDKKVLAGKLRLILLRGPLGNCVVTGDFDPAKLDETLNHFCSFGSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0X3</accession>
    <name>A0ABQ5S0X3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein L37a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RPL37a</name>
        <name evidence="4" type="ORF">VaNZ11_005755</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="101" mass="11345" checksum="A938952168648DED" modified="2025-10-08" version="1" fragment="single">CSYKDIRAKMSKRTKKVGIVGKYGTRYGASLRKVIKKMEVSQHSKYFCHFCGKYSMRRQAVGIWGCKACGKVQAGGAYTLNTASSVTVRSTIRRLREQVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1Q9</accession>
    <name>A0ABQ5S1Q9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_006343</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="25"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="761"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
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            <end position="1550"/>
        </location>
    </feature>
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        <location>
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            <end position="1594"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1628"/>
            <end position="1660"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1661"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1694"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1828"/>
            <end position="1860"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1861"/>
            <end position="1893"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1894"/>
            <end position="1926"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="2015"/>
            <end position="2037"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="2090"/>
            <end position="2122"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="2123"/>
            <end position="2155"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="2158"/>
            <end position="2190"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="2191"/>
            <end position="2223"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="2224"/>
            <end position="2256"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
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            <begin position="2282"/>
            <end position="2314"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="2577"/>
            <end position="2609"/>
        </location>
    </feature>
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        <location>
            <begin position="2610"/>
            <end position="2636"/>
        </location>
    </feature>
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            <begin position="2781"/>
            <end position="2813"/>
        </location>
    </feature>
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        <location>
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            <end position="2846"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="2945"/>
        </location>
    </feature>
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            <end position="2978"/>
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    </feature>
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            <end position="3011"/>
        </location>
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        <location>
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        <location>
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        <location>
            <begin position="1964"/>
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        <location>
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        <location>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="395"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="453"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="878"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1171"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1355"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1371"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1752"/>
            <end position="1771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1968"/>
            <end position="1990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2399"/>
            <end position="2412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="3186"/>
            <end position="3201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="3275"/>
            <end position="3291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="3317"/>
            <end position="3344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="3402"/>
            <end position="3415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="3557"/>
            <end position="3577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="3596"/>
            <end position="3624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="3638"/>
            <end position="3653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="3710"/>
            <end position="3720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="3738"/>
            <end position="3749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="3886"/>
            <end position="3908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="3967"/>
            <end position="3976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="4137"/>
            <end position="4149"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="4293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="4293" mass="425799" checksum="31D03CE203A1C183" modified="2025-10-08" version="1" fragment="single">[encoded data, 4293 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1Z2</accession>
    <name>A0ABQ5S1Z2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006839</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052881">
        <property type="entry name" value="Keratinocyte_PR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003882">
        <property type="entry name" value="Pistil_extensin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48138:SF2">
        <property type="entry name" value="KERATINOCYTE PROLINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48138">
        <property type="entry name" value="KERATINOCYTE PROLINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01218">
        <property type="entry name" value="PSTLEXTENSIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="5"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="866" mass="85403" checksum="79E7D4D949F1FC74" modified="2025-10-08" version="1" fragment="single">CDLLLQFNVYSECVVIGAEVKATINGVPTKVGPFFDEPYDGPNGSAVLRVTQLGLNMSSNGTKICLTLTANRYGRGCTTLEQLCIPPNGMAPGSCLTAMFDSTLDCCPISRTGTNLKLPQPPSPSPLAPSPSSPSPPSPSPPSPSPPSPSPPSPAPPSPSPPSPSPPSPVPPSPSPPSPSPPSPSPPSPAPPSPSPPSPSPPSPVPPSPSPPSPSPPSPSPPSPAPPSPLPPSPFPPSPVPPSPSPPSPSPPSPVPPSPFPPSPLPPSPAPPSPSPPSPSPPSPSPPSPEPPSPLPPSPNPPSPLPPSPSPPSPAPPSPSPPSPSPPSPAPPSPLPPSPSPPSPAPPSPLPPSPSPPSPAPPSPSPPSPSPPSPAPPSPSPPSPSPPSPAPPSPAPPSPSPPSPEPPSPLPPSPNPPSPLPPSPSPPSPSPPSPAPPSPLPPSPSPPSPEPPSPLPPSPTPPSPLPPSPSPPSPAPPSPVPPSPLPPSPAPPSPLPPSPSPPSPEPPSPLPPSPTPPSPLPPSPSPPSPSPPSPAPPSPLPPSPSPPSPKPPSPSPPSPAPPSPLPPSPSPPSPAPPSPYPPSPSPPSPAPPSPLPPSPSPPSPTPPSPSPPSPAPPLPSPPSPSPPYPAPPSPSPPYPAPPSPSPPSPSPPSPLPPSPSPPSPSPPSPAPPSPLPPSPSPPSPAPPSPSPPSPSPPSPAPPSSSPPSPFPPSSSSPPFSPPPSPLNTPNSSCAMCIDVLVDVAHIFPPYQFDAATCDRIQKRISFDINEQVIAQGLTPLVQNFSVDPSKCSLNKVSICGTFASEGDAAKLESWAQVQAEQFWIFAASDVCQPTMYGYTFQITTDACLAISEARTCTPGPSDFPFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S260</accession>
    <name>A0ABQ5S260_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="783" mass="83158" checksum="004025FBA038C18B" modified="2025-10-08" version="1" fragment="single">MACGNRLLASGSRRRAGRLQSLASTLQGTDLRFSGHFIYSTLPECPVVGNPLRCTNRNRVMDWLAAQGAVEAADTSRLHIPPLRPHELELLESSDPLYSRPPEAVLDRQYVSWREEVSVLPDGRHLKQFWLVAAPGRGDCPDRLVVTAEDSHRRDRRYTYKALQEFGGFVFENGNCAKEWLDFIVGKLSRPAPRTVKITQTSRRRAPPNPGRRSHPFSRPLASPTSTSVSPLALPLVPGHHTSSSGGDSGATTGPSLPVPAHKASSLQENFGGSSGGGGGSSGGNSGGGGGGSSPTAVSLPASTKLGLPPACWDSQASGPVGHPPHAAAFAQELRQTEATSPSPSPQQHAQQQHQQQPQQIQVGEASPTAGGCAWAFPISPWVPQQQQQQQQQVWHRLASEDTPGLWTPRRTSTGALPGLRISETTSNGLERPDHTVSARQREQATVSPEGTQLRSPHPAETRLETHLELHFPGLSPLDATNGPVTSRRPRAAAAPCLPSDLQIAGQDHPMDEQDFAEPPSMDPVPEPVQPSPPPRQTQMHEPPPPPPLPPLPHDDLEEDEATRELTQWLLYGDDGNDDGGGAGDICPSDVPAATAQEVLGEPAGGPAASDAVAAAAAIAAPSWACMTEEDSVSCCRVFSPEPGDPADSPGLLAMMMDCCLVAPDPEPDFDPYTAPDVRAAFQRSSNGLLDWVTEVPDAETTAAFRFWAEQLAVFAAFHAALPSTATNMGISPLALKHTHLRAAAQPPMPDHAACDMDIDYPIQPSPPVGATAMRQAAAGPNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2C4</accession>
    <name>A0ABQ5S2C4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_007184</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10230">
        <property type="entry name" value="ASKHA_NBD_HSP70_HYOU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF3">
        <property type="entry name" value="HYPOXIA UP-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047052475" evidence="8">
        <location>
            <begin position="22"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="567"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="897"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="591"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="603"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="646"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="934"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="955"/>
            <end position="1070"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1070" mass="116656" checksum="2503920E7FAB046B" modified="2025-10-08" version="1">MRRGVALAVACVALLAQVATAALMSIDLGSEYLKVCLVKPGRTPISIAVNEMSKRKSPALVGIVNGERLLGEEAFSFAVRYPEFIFQRARDLLGKDPDDPTIAAMLSEHGLPYKVVPHPKRGVASLQIGEDAIYSPEELVGSILYYARQVAEAQAGGPVLDAVISVPAFFGQRQRQAMADAAALAGLNLMGLINTHTAAALQYGIERDFTNNSQTIVLYDMGSGTTEVALVKYSTYTVKEAGKPRVYNQLEVRDVDWDESLGANLLDMALAKHFALQFADKTKLKVDVTALPKAMAKMRRQVRRTKEMLSANSAAPCTVEELYDGRDFQSSITREEFESLADDFFNRAVAPLKRIMERNGLQPGDIDAVELLGGGSRVPRLQAALSDVLGGRALDRHLDADEAIALGAGLLAANLSTSFRLRKFGMVDMTMYGISLTLDHVLLAGGEEEAVVEEPGGEPFMKVRNLLPYMKKLPNKRVVKLERLAGDPLRFSLAYNITTPHGLPPGIKTPELAEFEVTGIDGVIQRYNTSGQVSLRFEADYSGLLRLDKVEASVEYEVMEEKIIEVPVDDGMNTTTSAGGGEASGTPAPAKDADEKGNKADTESDTDDNDSSEGVEENGGNGKDSDEGGANGEGDVEAGYEAQDRPQGSQNGSKDNTEAPQTNATANVTGADSSNTTKSATTIKRIQVPKLKVAKVPLKVGGRGWLSPPLTDTDMSEAKQVLATFVAAETRKREVAKARNDLESYIIAMKEALETDELIQKVSTEDQRDSFRTRLTDEEDWLYMEADESEGAQQFIYRLKQLREIGEPIKRRAEDLEMRPKLVQELRNALELQRSIVKAWPDTKPWISEAEREEISKLVSGLEMDLEAKVAAQSSKADHEDPAFSVSELVQAWERHEKAFNKINNKKKPKPPPEPKPAADTNTTEGAGKGQGAAGDTAGDTTAKSESQGAGGQGHETDADNKGATKDEPKKAGRNEDTQKETKDSKSKKEEPKKDSKSKKEEPKKDSKSKKEEPKKDSKSKKEEPKKDSKSKKEEPKKDSKSKADSSAKGSGKKEEKKSWSWNKKGDSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2P5</accession>
    <name>A0ABQ5S2P5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small-subunit processome Utp21 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006952</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059157">
        <property type="entry name" value="WDR36-Utp21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007319">
        <property type="entry name" value="WDR36/Utp21_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840:SF12">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25171">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25168">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04192">
        <property type="entry name" value="Utp21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR36/Utp21 N-terminal" evidence="6">
        <location>
            <begin position="33"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="294"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="529"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="612"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="WDR36/Utp21 C-terminal" evidence="5">
        <location>
            <begin position="797"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="718"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="859"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="505"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="728"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="767"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="870"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1060" mass="113595" checksum="439D17638CF15B76" modified="2025-10-08" version="1">MEVVPLFKPFRALGYITDNVPLAVNRLGKETFVTVSVGKAWQVYNCTKLSLSLVGPQLSGDIRGLACASDLTFAAVGSDIVECKRLHRSGVYRGAHAGHVRQLLVLGRLLLSLDGVSEKDSRLVVWEIGKYDEPLRVIEFRGGFQPSCMCHPDTYVNKVLVGSEGGEMQLWNWRTGDLLYCFKGWGVPIRTLASSPALDVVGVGLADGRAVVHNIKFDEEVVSFANAAGTGLGADRLLGGGAGTAAARGGVGGGACTALSFRTGAGLPLMAAGGGGGAVTVWNLEERRLHTVIRDAHDGQLLSLHFFPGEPLLMSSAADNAIKHWVFDSADAMPRLLRFRAGHGAPPTLVRHYGEGGLRLLSGGCDRSFRVFSVHQDQQSRELSQGHIARKAKRLRVRQEDLKLERVVDMDACEVRERDWANVITAHEGDPAAYSWRLVHFSRGEHILMPPLEELSGCPAAAVTSVALSRCGNFGIVGSASGRLDRYNMQSGIHRGAFYRPAGPEGVGGGPEAKAGGGPAAMQQGKAKRNAHNSAVIGCAVDGSNRRLVSVGFDSVLRVWDFRTQSLAAEVALGSPATRLVLHPVSGLAAVALTDASLRLYDIEVPRLVRRFRGHSDRVTGLHISSDSRWLLSSAMDGTMSVWDIPAAQCLQVMKLGSPVTSLSLSPSLDLLATTHVNRRGIYLWSNQALFGDPAAIPHSTLAVPVARIALPTISDGRNTRVRSTTEERDEAEGEEADIDHEDEDGVSSENEDAEMRDVGAGARTAEAVDGRDGGADASRSKPYELRDPVSGAPLPLAPELVTLSLLPRSHWDSLTRIEVIKARNKPLQPPKKPEAAPFFLPSLPGLSANPIVVAAEGQEQSGQGVNQEAADEDMEEAEEAEAGPNSRVIRLKRRGGRRAIGNAAMSGPVSPFVHQLRLAAVAAEAAAVANGGDCGTSSFVDCYGPVISLFRKMTPTAIDRELRAMQILEGSCDEQSEIRDLGALLDMLIASLGARRDFELCQALLNVVLTQQGDVLCSRSELAPRLQRLRGLVAANWQRLDSLMQHVRCMVGFLGNLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S371</accession>
    <name>A0ABQ5S371_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007381</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="5">
        <location>
            <begin position="11"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="441"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="651"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="725"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="656"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="725"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="882" mass="94717" checksum="1B07B3148055CB57" modified="2025-10-08" version="1">MELRKRRPTARFGDAVTLDVVLNIVERCRHIILFTGSGISAASGMSMFTTSNGLYERARKKFKVQDGIKLFTYSFYRQRRAEVQDFFAQIYSEAQASRAATGHHAMAQLLQLGALQRHYTLNIDGLSEVVGMDTWHHERNPQGSTVEMHGNIRQLVCPTCHAVEPLSRHNINVMRAQQPVPCASCGQDQLRFKVMLYDDGEGDCITPEHVFDVLEEDLKVCDMVVWAGISFQQSASVEYFRRVRHMLGTLGRLNAVPQVLINPSEEPYFNLLSAMSNPNELQLLDLRETSDIAFPAMAQRVQQAIMCEDDEYSKSVGVHMSSVNNGVHPGAGAFTAAAAAARIEPTQAAAMAAKGEDLGPAASECAAGATELRFGAKTTEVPVLSSIKLEAGYSDDVGTRSGILSEGCLVNIEVPVDATASASGPEPEPTPVRSCHIARSARVSRSAEKVEEGEAFFADDADDEDYIEGVEEEEGRCRRNGRGRGRGRGSASRGKGRGRGRGRGGGSAARAEAYLSASSSQLDQRPLSPGQISLGQLLGVTPGTGVPGAVSSERSLLPLVQPGLRLAFGGLPQLQQQQLTQVAQHPQLPLFQPLQKQEMLQQGVQPLQQLQQFLQLQQLAQLRQQQQPYSLGLLQRLHALHQQQGAQQQSPALNVQLAQSPQQQQQDQHVQISQQAQGATSLQHIQQLSQVQFVSALSTLPGSVVGNGLLDAPQSQLLGQDALTPQQEQPHLSNEAQSVQAHDQPAQHERLVGFQQRTLLQQNTHGGSQSGLNVSGVWPDWFTPGNGEIASRSVAALNPVTSSAEAPDPAWGGSGMGLLPLAAFGPSSVAQALTTVVSNLEAPMHGVFGLGGHCGALSSGATLNGPSVALPVEFARSKADTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S396</accession>
    <name>A0ABQ5S396_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_007427</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="458"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="11">
        <location>
            <begin position="71"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="512"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI64229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="538" mass="57387" checksum="225E521BE1F300EB" modified="2025-10-08" version="1">MSASPIATAISSCDALTKTSLGDIGLNKDEISRLCSDGCRDFQDCLNVYLLGQTAATKAKAFEVSDSLDVAFMLYNSYLVFMMQLGFAVLCAGSLRSKNCMNILLKNVLDSCVGAIGFYLFGYAFAYGLKPHDASNGFIGNWNFALSYTTKASHEDSDDFATFGWHNFFFQWSFCAAATTILSGAVAERCNFVAYLLYSFFLSSFVYPVVVHWVWSPHGWLSASNTVHDGNASILRVGAVDFAGSGVVHMTGGFASLMGAWIIGPRVGRFAADGSVNEMKGHSATLVVMGTFLLWFGWFGFNPGSNLVIASATATRIVSRVAVCTAMSGGAGGVGMLLYKYVTTHSWDTVGTCNGVLAGLVAVTSGCAVLEPWAAIICGLVAAVVFCIVDHFTLYVLKVDDPVSAFPLHGGVGMFGVLFTGLLARADYVQEVYGSTEWGSTAKEGKRYGILYGGHGQILLAQFVEVLSIIGWSCFMMGTFFYTLNKFGLLRVSVDAELAGLDITNRLKSGTPACNGLPENSLPGSSGERQQGEKGLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4T7</accession>
    <name>A0ABQ5S4T7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BOP1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_007501</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028598">
        <property type="entry name" value="BOP1/Erb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012953">
        <property type="entry name" value="BOP1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08145">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01035">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BOP1 N-terminal" evidence="9">
        <location>
            <begin position="180"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="456"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="536"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI64281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="555" mass="61171" checksum="4E72BDE96C488597" modified="2025-10-08" version="1" fragment="single">MSKRNAKREPVREADSDIEDELEDLNLEGDASSDDADSDVDADGDEDGDEEETGTTSDAGEDDEIDDAILELMAAQAERAQRKAAARMPNSEGDKAGARRQPPTRTAGKSRMATAAESVAEPDADAAVAGAKEAAAVAAGEAEDPEDADEDGRIPDPGSDSSEDERPNRNTVGAVPLEWYKDEEHIGYDIDGRRIARTARKDKLQQLLDRNDSKKALRTIYDPYNDEEITLTKEELAMVMRIRNGQFPHLEVNPYEPYNDYFTREREVMPIVDRPEPKSRFVPSKWEEKKIVKLVRAIRKGWIKVEPQVRQDPDDPDAHQPPAYLLWADDGTAEGAGPGKSGSGLTYIPAPKPQLPSHADSYNPPAEYLPTEEEIAAWQMADIEDRPRQLPTSFPSLRAVPAYTDFIKERFERCLDLYLCPRIRRKRLDITPEALVPKLPKPRDLQPFPTTLAVSYEGHEGPVTSIAPDPWDGQWLLSGGQDGTLRLWEVRTGRCWRTWRLGAGPVAAVAWCPNPSLRLISAAVGNCVVLMASGTGPEGAEGEAEQVLQAATSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S591</accession>
    <name>A0ABQ5S591_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008241</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="502"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="532"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="577"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="640"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="757"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="780" mass="83684" checksum="5CDB5D86A904459A" modified="2025-10-08" version="1">MAFLPRSSKSFEMDHGSHDSVDRRPDSCVRLTTDEESKSPRTASQPTVPTDLANLPNIGDGGNTINNSREEQSGRRHNNGVDKSRLPSLRYTVIDVPPWHVCLVLGFQHYLTMLGSTIVIPALLVPAMGGTPKDHARVVQTIFFVSGLNTLIQTTIGDRLPIVQGGSFSFLKPAFSIIAIIKSTMEFRSEHDRFVYTMRELQGSIIGSSLVVMAIGYSGAMGALLRFVSPIVVAPTVCMVGLSLYSAGFPEVTACVEQGLLSIVAVIIFSQVLKKVEVPLPRWLLPSRFPPLLGGTDASPSSPLVDLGRTSSSGRRRGGGDGGSVRIFELFPLLWSILLSWAVAGILTAAGAYDNTSPERQASCRTDNMQAVNVAPWLYVPYPLQWGMPIFRPASIITMLAGTLAAMIESTGDYYACARMCGAPVPPPYVISRGIGAEGMGCFMCGLFGTGNGTTSYAENIGAIGLTGVGSRRVVQAGAAIMLFLAIFGKFGGMFASLPPSIVSGLFCCVFGLIAAVGLSNLQFTDQNSSRNLFIVGFAIYMSLSIPYYFDSYTSVHGGIGPIATSSHAFNDIANTLFSTPMCVALICAFVLDNLVSGTPTERGLKHWSALVGTVETHKRAAARPDDGVAEDAATDMDVHSVTPTGGPCTGAGGTLRDAGDYSTDSGSDDDDPSMDPRNDVRIKAVYDLPHFLQLVNDRYILPRRRAAQLGLRRLSRVVRQRWRRLVRRMLACCGSSAAWIFRGARCDRSVRVPTSASSYATSDDDDATLPGTAPYSNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5V9</accession>
    <name>A0ABQ5S5V9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="156" mass="16586" checksum="707C91FE5E97404E" modified="2025-10-08" version="1" fragment="single">CLSGCRQFSKPCMSLRHSSACPLKAAGSGPCVCCALERQLREMLVSADGAALSPDEVVSVLHRISPAFKPGRQQDAQEFWVKLLEMLGQEASLCALLRSSAAVDVATHQAIRVAAAAAATCEPKDLGSVECTERIFTGMCQNGRRCTKCQQVTSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S633</accession>
    <name>A0ABQ5S633_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(+) diphosphatase</fullName>
            <ecNumber evidence="4">3.6.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_008863</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + beta-nicotinamide D-ribonucleotide + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60876"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:60877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. NudC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.22" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03429">
        <property type="entry name" value="NUDIX_NADH_pyrophosphatase_Nudt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049734">
        <property type="entry name" value="NudC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015376">
        <property type="entry name" value="Znr_NADH_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001299">
        <property type="entry name" value="PRK00241.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF6">
        <property type="entry name" value="NAD-CAPPED RNA HYDROLASE NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09297">
        <property type="entry name" value="Zn_ribbon_NUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="11">
        <location>
            <begin position="259"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="439"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI65320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="498" mass="52973" checksum="280E75BCD6800344" modified="2025-10-08" version="1" fragment="single">PPGAPPMASTTSGGALPKGRISMADMQSLLPVTISQQLLPPQHFSGNPLDRTYDRRLKFDGTSKSEEISLVVVAGREVCVRESSSGLRSSSSTGAAGGGTGGTNTTTTTTSGGAVSGPPAADLQALLLNATDPELDLNSESICLTWVFGDAAYQLPLYLLGQDRTERWTFALDVSDCRTGFMEFLRDTCSLGAGVGLSDLRAVLPQLSRDAAAIAGQATALSQWHQSHKFCPRCGAPTAPVEAGLRRQCTSAPHHKLYPRTDPVVIMLVESPDGRRALLGRNKKFTPGMYTCLSGFVDQCESVEEAVRREVFEESRVLVAQVAVVGSQPWPIGRYGGCELMLGCMAKARSYEVLVNMDQMEDVQWYDKDELRAAVEMYDVMGAPSEADAVAEPPMSVAQLQEHSLEQLGFFIPPPLAIAHHLIRTWALHEGPWFPTAGGVGGGGAANGSHSHPHGYPHSVPMSTAVTDGSIFTPGAQGQGQGQPGGVQEPVMYARGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S678</accession>
    <name>A0ABQ5S678_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008787</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43021:SF2">
        <property type="entry name" value="CATION_H+ EXCHANGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43021">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="7">
        <location>
            <begin position="31"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="498" mass="52851" checksum="7E99BD8A14C0C00F" modified="2025-10-08" version="1">MSDTGASTALSSPVFQHFLVACSVAVIIAPFSYYMGIGARMLKLPQITGYLVSGIVCGPYLLGILSTESVVDLNIIEGACLSIIGLAAGAELHLSELSRSKKQVLGITFGISAVTWLFCYGALDYTSGLLPKLGELDAPHVVAVASLGATLMMARSPASAIAVLKEMDGKGPFSSLVMAVVVVKDVVVIIAYALNMEFIRVSILPSATGSPHLLGMVLPLMSVMLSITVGLCGGLLLSMMLRPHSLLGAILPSMAPAATFKVKQLVILLVSTLIFNMAHYFDAEPLLACVTMGLFMVNRRHERADKEKEELHMMVSQIMSLTNVAFFGLAGASLKVVAMKDMFGAALLVFVVRLAAIFIGSWLGCYSTSTSAEYRRLFWMSMITQAGVAMGLARLTGTRFPDWGPHFQTYMMAVIMLNLMVGPPLFRQALVRVGEAKALLLPIKGSGVVAAQQLQLGSIIIKENGAGVSSSEGGLVHQHGNGLGGEDSSSKSDRDLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S693</accession>
    <name>A0ABQ5S693_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrase SAM-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008527</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="41085" checksum="81BF816D53C95310" modified="2025-10-08" version="1" fragment="single">TVPHYALDMRLATAPPLVTGLLVEHLQAAAPGRDHSLRLEQIATVIMARIGTKTWQSYASHFSAFVRFCIGEGVDFLPASRYTGLLWAQFLAARGTVQARTAQPYFSAVNTVHDLLGFPKPCDGDNVLLSAFRRGWERLQVSLTPPSALVLAFSAIDAWSLYERLPSVPVASDLFIPLLFVVLSFCLFLRPDSLLSVVWARVSLVDGESTFQYKPLHWKGRLVAPELAPVLQFPLGNLPHLRVALSQLFARDTSRLWPVRQTTQGAERWFGRVLSQCGLERLRPVHTLYSLRRGGASWILSKPSAAGPQVPQRCGSTTSTWGFVAALMRSRFLALSRPGASHQWRDSFSTAERGFLFLWQNLVAGANAGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6Q5</accession>
    <name>A0ABQ5S6Q5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008386</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FUN14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI65017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007014">
        <property type="entry name" value="FUN14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346">
        <property type="entry name" value="FUN14 DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346:SF10">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04930">
        <property type="entry name" value="FUN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045435114" description="ABC transmembrane type-1 domain-containing protein" evidence="7">
        <location>
            <begin position="27"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="21911" checksum="0408766FB549FFE7" modified="2025-10-08" version="1">MRFHFKIRGWVWALLQVAVIIVTCYGCNRALSASCVDGINCDVESLHNKTAFQPRRSTRRAKEARIEAYPGAQVTAFIAEAWPIVRQLGINGIVGYACGILVRRVIQMMVFGLAFVISAVQILAYFNWVTVHWDRINSDLAKLLGEGRDVTSSESTGFDAPTLFAMAFERLATIFSTGLPSLAGFATGLTLAFMPGMALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6Q6</accession>
    <name>A0ABQ5S6Q6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CobW/HypB/UreG nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051927">
        <property type="entry name" value="Zn_Chap_cDPG_Synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603">
        <property type="entry name" value="COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603:SF1">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="2">
        <location>
            <begin position="372"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="570"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="882" mass="92159" checksum="CB2BE6649DEE53B6" modified="2025-10-08" version="1" fragment="single">MVRLHELALGDTFEDLLVEVWQLEGVDPASKPRDPVYSALIRDPSAYCRLIYRANNNCKPSNVKRGKFYRISGKVSSYRGRMQIQIGSSWGRVEKVNGEEFVCTLPYSPATDLSAMERWYGALVVHASCGTGPGVKHGESIPRLRAYLLSTTRQQEQQQQHQQQRRQQPQPLVSDGAVVGLLPMPPTPVPCTTAAYVETERGAAHRALRDVLRVAPDSVRILPLPPVLHVQPATEGGANPGAPAFFYIYAAVLQSSDEDLKYVFGEARGGGWADLDQISNVSAGRSPAAWHTALTRLVEMTRLAHAAGLVDLHLPQGSDMAAAAAAAAGASEETPPKVPAAAVVAAAAPVAADTVSSPPPSLPTVPEKRLLPVTLLSGFLGAGKTTLLRHILTNRDPNMRCAVVVNDMAELNIDAALVTHGSITTATVGGGAAAVAAAPERLVQLQNGCICCTLRGDLVQQIAEIAADGQFDYCVVESTGIGEPMQVAETFEFPLDGSDPAAALALEAAAAAAGAAVVHGSDSVNGADGAANSLSAPRRLADVARLDTCVTVVDAANLLDNLHSLETLKDRQESEKVHMHGGGRRDRAKVRDGDAGADSAGAGCASTTLTGRPSGGPAKGSSCQGGEECDAAQKQGHHGHGRHGHGHGDDGDEEDEEEEELERNVADLLLDQLEFADVILLNKVDTVAPDQLPRLMALVATINPSARLLPTLRSEVPLCEVLNSGRFSLERARDGAGWLKAIREGTDLVPETAEYGITSFVWRSRRPFHPARLHAFMDKYFVLQQPDWSLADGGDGISGGSGASEMLHSGPPPPVAEVACSGGAGASAVSSTALLGDEVAAAAAKAQEAAAVLAGVFAGFNGGAGVVAGSKAIAGPDFSLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7J1</accession>
    <name>A0ABQ5S7J1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008961</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051601">
        <property type="entry name" value="Serine_prot/Carboxylest_S33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248">
        <property type="entry name" value="2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248:SF14">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="98"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="378" mass="41515" checksum="1C9D8E5F54F04121" modified="2025-10-08" version="1">MLRARPSLASMRTQLRSYAGRKEYHGRQARLHCSAGLITASSPEAVAGASSRLPPSFLRSVSYDDLDLDTSLAYEIFQGPLARWTHPNNQQQAPPTAVMLHGILGNRKNMASFAKMIVEGFPSWQVLLVDLRCHGESASLPTRPSGPHGVAQASSDVLALLQQLRLFPRVLIGHSFGGKVVMSMVQQFPKRLPRPVQVWVLDSLPGQVRTGGGTEGGDHPGALIDFLRSIPMPVANRNDLIDVVVRQGFSTNVARWIVTNLRPVRSGTAYGSGPLTWTFDLDGIAELYNSYENEHLWELVKRPPEGLSLDFVKAERSTFRWGGTDEAAITEAGHRVHLLPNSGHWVHTDNPLGLYDILAPSFGGSSDLKQWRSSAVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7N2</accession>
    <name>A0ABQ5S7N2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ferritin</fullName>
            <ecNumber evidence="8">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_009405</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the ferritin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF75">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0408">Iron</keyword>
    <keyword evidence="2 8" id="KW-0409">Iron storage</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="10">
        <location>
            <begin position="151"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="53"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="201" mass="21395" checksum="6E376C8432B016F2" modified="2025-10-08" version="1" fragment="single">MQLIAPFATLAMAPAHEPRLRGSLSAGRTFGTCASAAAALHTVQFRSRQRRSLLNTPAAVPPLKSEEPDGGVGAERNRGSASSDPVVQRAAAEARQAPPAVQRGHRASTQTGLVFEPMGEEVSPLVADLDAQLMDPAAAPGLSARRSMARSGYPVNLEAAVNEQINIELTMSYIYLAMYSFFARDDVGLPGFAAYFRHNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7S7</accession>
    <name>A0ABQ5S7S7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051412">
        <property type="entry name" value="Formin_Homology_Diaphanous_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691:SF6">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="638" mass="68491" checksum="18DF529B4235C3E3" modified="2025-10-08" version="1">MLFEFCLDMLKGGCCLRKVLNLFIACLILALAFTSRVRGARKLQQDGLNDEDILKGAENFNPQTESHMKAIEAAIATLTAEESDSLHRMLFTTAATYPPSQKPNAPRPPKASSPPPPLPPGLAMGLQDYSAKFVNADITTELNITVEESVQQFISRFKNITSHFFNISADDIVIRGVYKGGQRLTRRLMLARAERTWTHHQLAAGLPPGSNMRLLIHVRSYAADGTSIQALRQAELALLYPSFASAATTTAPNAAAGIADDPSALTGIGDRALQEADDGGVVIVEFTVVQYVEVSLPPSPPTSPPLSPGQVAPPNTPPSSSSPPRRPSRPPPMHPFLLDRLADELGATKVYITPRPPSPSPLPPSPMPPSPIPPSPAVSSPVSPSPEPPSPLPPSHVPPSPLPLPPLSPSAQGTDTTINVTDILGQGSIVSKLNRGDTVLAGTVVWYDNANFRSFRKPHSALNLIFADKRLPCAVARGVCAPCPRAWAASRDSRVVPIYFREPIQLHTIKILQLQSPGVIIVELLPWPATIIPELPDLAPLSGTLGAPVWNVDHDPSDCGSDLIIRLPMERSGINLSVPPYGTQGNLPRGLRGTAAGGLLITVKDQLPKQKATVIESVRISGRILYPRDSRKFSRVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8L4</accession>
    <name>A0ABQ5S8L4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF45">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="41663" checksum="6CDB2C6C0A766CAD" modified="2025-10-08" version="1">MSEQLAIYRRCRRQYPGTAACVLLPAFCRRKAAASLRGMRHVHRFEPGTVLFNGVDWEGTLAPWPGTSYSVDVWYEAPDGGAVGYGPALSALTLSAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVAEMNALGLSPESSDCPQVTLADGGKHPIVGRITLSLSIGPLRVVTRPYVLQGLAETAPYIMGSSTLRQYRARIDMETATLHMCKGTLSIKVPFLSFEHVTPKEGETEAVPMVNFAVTAMAARIESELVGRKEAVRLLRKGAHGLLLRPRVSCCVTTAQISADPEVEALLSQYEDVFKDIPGLPPMRPVDHTMPLVPGVQPTARPMYRLSPLELDEVKRQVTDLLAKGMIRPSTSPYSAPILFVGKKDGTLRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8M6</accession>
    <name>A0ABQ5S8M6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyl-accepting transducer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000029">
        <property type="protein sequence ID" value="GLI66267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="43711" checksum="271F681064193230" modified="2025-10-08" version="1" fragment="single">LQLVDCAQESADEHGEVQQREAAAAELRATVVELEKRHEAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAGAAELRAAVMEGEKRREAAEATIVQLRAMVVKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8P6</accession>
    <name>A0ABQ5S8P6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000030">
        <property type="protein sequence ID" value="GLI66280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="106"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="1854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1893"/>
            <end position="1981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="708"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1645"/>
            <end position="1661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1662"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1738"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1758"/>
            <end position="1828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1829"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1966"/>
            <end position="1981"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1981" mass="200020" checksum="0E324F53625FC601" modified="2025-10-08" version="1">MAKWGQRFPALVNALGPLLGNYVFGNPLRRRTSGPRNRDNITVLFVLLFAFAFDGDICYGEVEVVAAATQGYKAREQARRPWVLNGKGEQQQGRVARHLLQSEDAFPCQDLCSVGCSESPWELSGVTRMVRGIASNTFCLQLRRQGVCEASPCCDLLSNGIDSLSVSVASMGRCNVTSVLRVFIGGRQVRPFVNYPLQYSDGVPSDVGLSIYDMTTLGLSADSLEVCFELRKACADWTDFCRPGIPSSKAGCRFAVNEANADGWYMCCNTCPVGTSTPTESSRTAPAVVIDQLPTAKSPLNRRPAPSPPPIVSPPPPKQSPRSQPPVPVQSKGTPSQLSKRPEPPPDLNRPRPPPPPSPPLQPSPRRTPPLLSPPPPPLPQPPTALASASKPIPAASSPAPPRPTSQPFRIPTAPLPLSPRPRSPPPPAPLSPPPRLLLSSPSPPQPPSPAPHPSPPPPPSPAPHPSPPPPPSPAPQPSPPPPPSPAPQPSPPPPPSPAPQPSPPPPPSPALHPSPPPPPSPALHPSPPPPPSPALHPSPPLPPSPAPQPSPPPLPSPAPQPSPPPPPSPAPQPSPPPPPSPAPQPSPPPPPSPALHPSPPPPPSPAPQPSPPPLPSPAPQPSPPPPPSPAPQPSPPPPPSPAPQPSPPPPPSPAPPPSRPPPPSLPSLPTPPPLSSPLPPPPLPSPPLPPSPSAPPPSYPQALLSPPSLPSSPPPPSPPPPPPPPSPPSLPPPFLPSSPPPPPPPSPPPSPPPPPPPLPPPPPPPPPPSPPPPPLPPPPSPPPPPPSPPQSPSPPPPPPPPAPPPAPSPPPPPPPPSPPPPPSPPPPSPLPPPPSPPPSPSPPPPPPPPSPPPPPSPPPPPPPPPAPSPPPPPSPPPPPPPPSPPPPPSPPPPPPAPSPPPPPSPPPPPPPPSAPPPPPPPSPPPPPPSPPSLPPPFLPSSPPPPPPPPPPPLPPPPPPPPPPSLPPPPLPPSPPPPPPPPAPPPAPSPPPPPPPPSLPPSPSPPPPPPPPSPPPPPSPPPPPPPPSAPPPPPRPSPPPPPSPLPPPPPLSPPPSPSLPPPPPPPPPSPPPPPPPSPPPPPPSPPPPPPPSPPPPPLSPPPPPPPSPPPSPPPPPSPLPPPPPPSPPPPPSLPPPPPPPPPPSPPPPPPPPPPPSPPPPLSPPPPPPPPPSPPPPSPPPPPSPPPPPSPPPPPPPPSAPPPPPPPSPPPPPSPPPPPPPPPAPSPPPPPSPPPPPPPPSPPPPPPPPSPPPPPSPPPPPPPLSPPPSPSLPPPPPPPPPSPPPPPPPSPPPPPPSPPPPPPPSPPPPPLSPPPPPPPPPPPSPPPPPSPPPPPPPPSPPPPPPPPPPPSPPPPLSPPPPPPAPSPPPPPSPPPPPPPPSPPPPPSSPPPPPPPSPPPPLSPPPPPPAPSPPPPPSPPPPPPPPSPPPPPPPPSPPPPLSPPPPPPAPSPPPPPSPPPPPPPPSPPPPPSSPPPPPPPPPPSPPPPPPPPPPPSPPPPLSPPPPPPPPPSPPPPSPPPPPSPPPPPSPPPPPPPPPPPSPPPPPSPPSSPSHPPPPYPPRPSPPLPLPFPPPPQPFSFPSPYPPSLPSPYQPPSPPPPPSLPSPAPPPSPPPPPLPPPPPSPLPPPPSPLPPSPPPPSPWPPLPSSAISSPWAAASLSPRSPSSPSPQATPKPPLSMPPSASPPPPQKGWGVSAPPPPSTRYPDGGVRPLKGPRPNRQPPKPSKRIPIRAAQPSPSRRTPPPPPPIPNTRPLQGPRVSLPPPRPKPPSPLPPRPSPRFPPQPHPPKPPSPHPPKPPVPRPSPRPPPRPKPPSPRPPPSPKKLNLPPPRQLLSPLPLIQPSAFRRPRAPFPPRLAWVRPPYATRTAQDLNPSFTGSQPPETVQHLLPPAEEAWVAPPYQPSGVGKGPSMPKQRSAKQAMSPPAWQPPPADYYYAPPATQPPPEGDLEVRQGKASAPPKPAATEEWEPPPPLDYVDTPPPSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8Y4</accession>
    <name>A0ABQ5S8Y4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_009646</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.170">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006602">
        <property type="entry name" value="DM10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040193">
        <property type="entry name" value="EFHC1/EFHC2/EFHB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086">
        <property type="entry name" value="EF-HAND DOMAIN C-TERMINAL CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086:SF9">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06565">
        <property type="entry name" value="DM10_dom"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00676">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51336">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DM10" evidence="10">
        <location>
            <begin position="118"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="DM10" evidence="10">
        <location>
            <begin position="302"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="DM10" evidence="10">
        <location>
            <begin position="483"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="615"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="218"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="682"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="732"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="807"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="690"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="746"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="814"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="829"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="909" mass="99801" checksum="13CA691A2686BAC6" modified="2025-10-08" version="1">MRRAAGYNSEDSRQWGPSLPNLPGVGAQGPMEPRPRGQTLRFVNGYRIAEDVQNLDPMNRMHKWNGRVQDMGPTPPFALHEAGHVQASQKEAGGQGDNRSSGGHTSQAARVPNWITYDRKVLGFGAYFQEDVQYSPLETWRVRKVTIKYYLENNQVEIVEARELNSGLSQGVLLRRHSALKDDGSPLRWSDLRVGDQLTLYRRTYHLVSADPATRTFYSEQGQEQPADEPYPEGPYDQHRKRLESSRSGRPPTRGSAGSPPRGPPLPADEESRSLPASPRGPGALFRLLHLMKLTPEDEARDGAVLRFVAAWDNTAELFGDVLPYAVFYYVSDGTFEVREVNRRNSGRDPFPLLLKRSRLPKVLPPVHGRPMSPETRRRLGIPYYDWRDLYLGATINVYGRRMMLYECDETTIAWLKKYVPSLTPEQLTPIQVDFDPFGPKRPPLSIPPNISGIGSEEDSLQNVLHLVPRPVVRPYGDYLLKGDKVLRFAAVMVPLGPGRPLVGPHDAERKFILSFHLGDGTLSVFEPRQVNSGMEQGTFLERTRVPNPATGQPYGDADMQVGAILEVFGRGFQLQDADTFTLEYMEGQSYRFPCADYDQVRSKLANWLALYPDQLPDQLKSRLAAADSNGSGYVTKYSLYDILTSLGCDVTPHEVITLVRQLGADRQGLLAEQLLAALELSPPPAEAPPPHEPEPQARVEEPTQQQEPPVPFPAPGLRTGAWPPFRRVTWGPSPVSGTGPSHPVNSANAASTTAGAAGAPYPRLGPTPGYPRVDIAPAHGPSSFNYHKLNAGFGNYGRRSSTLSAVAGTGTADDDRQYPRDTPEKNWRASSKNGASAPFGSNWTAPPASVDMTPAATPNAGGDSGSAGVKRPGGSSFLATGIKQPASLWQTTNMAFGGVGHATSFQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S948</accession>
    <name>A0ABQ5S948_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_009881</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="12"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="264" mass="28243" checksum="E008FC7537E8EED5" modified="2025-10-08" version="1">MSGGMDPSEYECAICQNLLLDPVVGPCGHDYCRSCLERWRATKSSSSSTVACPLCREPVPGELGVCVRLRETIQRFFPEEVKKRRLEVGERTGNLAKAARQQATQRGATASVVAPPVGAVQTQRWPPRLGVYVAPASLPPTASATPQPQARFIPLSWATAQTPQPLWQTPVSLSPTLGASSGSATPQLLSQARMAPVSPVTPWMATASAAVVVDPAQPMPSGAIVSPPDTWMPPASLSFSLGWHDPAETRGRRSLGRRRRLMSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9D8</accession>
    <name>A0ABQ5S9D8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_010348</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF8">
        <property type="entry name" value="SD21168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047284904" description="Sulfatase N-terminal domain-containing protein" evidence="5">
        <location>
            <begin position="25"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="6">
        <location>
            <begin position="30"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="193" mass="21691" checksum="538EF6B4973C8943" modified="2025-10-08" version="1" fragment="single">VNVKGKMKRFLNVMLCLGIMTVSASVARRPNFIVILVDDLDETQDSSGELAMPATKRLLRDRGVTFTNHFVTTPICCPSRVSLLRGQLGHNTNFTDVLGPHGGYAKFRALDLDREWLPTWLQKAGYKTLYTGKFIVDYTIRNYDPPPSGWTVFDALVHPFIFEYYSPAFSLNGGQPRMYPGGWSGRWVGPIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA26</accession>
    <name>A0ABQ5SA26_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_010639</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="55"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="291"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="600" mass="64139" checksum="746A9E05263088C5" modified="2025-10-08" version="1">MELGERRSKVDAFHARFNAKVARKASGNRVASSRPAQPLPPQSFNYDEEDEDFVCGGCGTAIQHGQSLWCGACKCTAYCNIECQRVGWKRRGHKAACKLLARAKALPPADLGRNYVVQLKLVGRQGLTDWAGHLQREGQTELCSMHREMAAVLAPRERVLFALEARSHFMLKFLVVDPSKRLEAHDLALFTLQAVLEPTADVPTLNMPREAPQRPYAIWIPGGDIADSACVDRPMARPVGQLLAGAGLTTSTWYVGGGFCAEAMLRPDVYGGGSQCRGGSLINTFVALDPDGYTEDEEDEDGDGEAKVSGSGAPHARGGGGGGAAAAFPSAAAFPPPAAFHSPLRVLMIGGLHLFGPDAGEGNDPFLTATAGALRSDVGAWVDVAMYKKGKGNQPAQRALAQRLMSGDYNACVVLGLGSGDSFRPRRPTYPWGNGPWRDVLTGWVRGGGVLVLHGERAAAAVMQEFFGLPWTMDRAFYSRTDHKLNVANPLLGDLASSLPRDYNVKACMLYDVPPWERIYATALGARTHSLPLMEGERVEPDKTALAAAQVGSGAVVFFGDVNWEPLTMATVAMLTRRLVTTCVQRAGDGGGGGVVSVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA60</accession>
    <name>A0ABQ5SA60_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35744">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35744:SF4">
        <property type="entry name" value="OS04G0464600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="476"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="60650" checksum="43EBF72DDAF2F1BB" modified="2025-10-08" version="1">MLESVAGSWGVLALPSASRLFIMQRKSRAIRTCTFAANYAAVPTAQRPRGFMPIRRTAGIGVGITWLHLHAHLQHIPASTRRSLFHLGGPDESLHTTPERWGHSPVQATGTSAVPDPVNSRFPDTQTTSPLGAESQTVSTWQRKRRRLVPVAPAPADPTVAITASEADRGLQPKRHQPKKTGEAGSRAGSGAQANAGDGAATAATSGATAAAIPAGAGAAGTRAAATGLLAKGQMRSPAPRERMAAQAGQHIPPLAVPHTTAPTRPHTPADGTTSATTRGHEGRSEDLVLSTGPVYVFWDLDNKYPETLDYRGLMDSMRKVLHQYGKVVEVRAYANYHTLNFVPDIWQEAMLRTVEAARVEEELRCPLCGAKVRGPESKLRTHFRQLHQRQHEKQLAHKPAAKKYIQSDAFARYQKAAKGVLSAKRRGYGLEGILSGLNVKVRPVPQGPQKADVLLQGEAMELLKEAGQRGAKAASAASASATSTTTTTSAASTSSAAPSASTSTSTPPCGPPPPPSSSPPVLVLVSDDHGFESLLKLFSSAGWRTVTVSNTEFKNAKERIPWRSLVWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBV8</accession>
    <name>A0ABQ5SBV8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine-S-conjugate beta-lyase</fullName>
            <ecNumber evidence="3">4.4.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cysteine-S-conjugate beta-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_011608</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.13" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.60">
        <property type="entry name" value="Hypothetical oxidoreductase yiak, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006238">
        <property type="entry name" value="Cys_b_lyase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043144">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_ah"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043143">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036111">
        <property type="entry name" value="Mal/L-sulfo/L-lacto_DH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003767">
        <property type="entry name" value="Malate/L-lactate_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01329">
        <property type="entry name" value="cysta_beta_ly_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF50">
        <property type="entry name" value="CYSTATHIONINE BETA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02615">
        <property type="entry name" value="Ldh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89733">
        <property type="entry name" value="L-sulfolactate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="826"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="826"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI67413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="911" mass="96031" checksum="E5C6A8B6F647C0D0" modified="2025-10-08" version="1">MSAIGIHGQGNFECAVSASGTRLAIQPRPVHSRAAPSRPVGERLRCTLPISPVKQQTFRSASFMALSSAGQPSVKQTEEVPIAELRTLCTKALKLLGYKQDEIEILNDVLFYAQLRDNNQGIIKITSGGLNKSPEARPPVIEKETRLSALINGKNSAGMLVLHKATQMAIERAKNQGFGIVGTNHTPSSTGALGYYVDLISREGLIGIVLAQSPEFVAPHGAKQPIFGTNPIGISIPAQDGAVTMDMATAAYAWFGLLEARAAGRPIPPDVAMNAAGRATTDPNEVLQGGAIRVFDRSYKGSNLALMVELLAGPLVGAAVADKLEERNWGNLVVAIDPGLLGDKEEIKRRVQVVLDRVRGAERLPGVDEILLPGERGNRKASARLAAGVMPLEVNMLSQLRALAAKYDTQGSPPGSPAKLAVATRLVHPKTKVVDPYGGSMPPLWQTATFAQPSATTFGEYDYTRSGNPTRTMLEEQFAELEGGDRGFAFTSGMAALAAVCKLVKSGEHILAGEDIYGGTSRLLSQVVPTAGVSVTNVDMTDLEAVRAAIQPGRTKLVMVESPTNPRMQICDIAAIAALAREAGAITVVDNSIMAPVFQRPLALGADISMTSGTKFVGGHGDVTLGLLSVKGQELAKRVYFLQNAEGAGLAPFDCWLALRGLKTMALRMEKSAANAAALASYLAKHPLVRKVNYPGLPQHPGHALHFRQASGAGSLLSFETGSVEASKIIVEATQLFKVTVSFGNVNSLISLPCYMSHASIPAEVRAARHLPDDLVRISTGIEDPQDLIADLDQAMTLAMEKSGIRSATSPNGAAAASPQSTWATITSASSNNNNSSSSSTSGSNGSTPATVTYSFGNGTGNGNGSKPAPRVSVTGIVPASDREQWLLDRIRSLEAQLQEEQGATSQSHAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC40</accession>
    <name>A0ABQ5SC40_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Urease accessory protein UreH-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052776">
        <property type="entry name" value="Chloro_ReproSupport/MetalTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33876:SF4">
        <property type="entry name" value="CHLOROPLAST PROTEIN FOR GROWTH AND FERTILITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33876">
        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="38345" checksum="67A7555887A06C27" modified="2025-10-08" version="1">MLAIGSSVTAPRRVVAGVQQAALPSRNSVVCRRPTQPLAWKVFSEHSIRSLTCKTPAAAFAAAPCLPTFEERPIEHHQVGIFEALQKCRHIFSCLAFCAVYAYLSWGLPAGPPFASASLAIGDAPATGLTSTAQSAWAGLAAGFLHTLCGPDHLAALTPLTIGRNRFAASALGALWGFGHSTGQLILGLVFVVLKERFHDLVPFLSKWAGTVVPLTLIAIGMMGIYESFFEKGDAHAEAEAEAVGLALAGGGSVSASRSSAVSSGLKAGFATYATGIVYGLQPDALFVVIPALALPTKLAAIAYCSMFVIGTVSAMGGYTLLIGTTSQALIKEQPWLQKHLSTVASGIAIAVGLLMLLAGMGMEVPFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCA8</accession>
    <name>A0ABQ5SCA8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nucleoporin p58/p45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011744</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024882">
        <property type="entry name" value="NUP58/p45/49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13437">
        <property type="entry name" value="NUCLEOPORIN P58/P45 NUCLEOPORIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13437:SF2">
        <property type="entry name" value="NUCLEOPORIN P58_P45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="469"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="51025" checksum="C5DFE0DE606E3D1D" modified="2025-10-08" version="1">MAFSFGSTAPATASFMGSLFGVPAASSASAFGASTAPAFGAASASTFGVSSAPAFGAASAPVFGPSTAPAFGAAPSPVGGPAAAPTAPNLFGPMTTNFGTAGLFGQPTSAPATSNALVPAGGLFSAQPQQQAQQPRPGLNYKTKFEELPPQIQQELQKIQQQISLYRDECKALDNDPRLYDTLSMKQNLDSDTAALRQTLQSLYQAIVADDEGLNSFKERVTVLLRSTEAAIRLYQRSKMWRDVSQASAPGSQQQLLIQSIQEQMSQPVVLPNPYLELAVMGFSAAIEQYHSCISELERVMQAAVGNGTVDEAAAVLNLPTLVSHMHDYFVHVAARMERLHKEVARAQEAYLARQRARGDYSNPFEEARPLHHLSAGPAKLAGGIGYFGSNGPAGGAAGGVGANVNGFGGATNNMPPVQTSTVLVLGPPGGNTAMPSGGGGIFGGLSPGAFGSAASPFGAAAANAAGAFGTPLQQPQQQQQRPTAARTGSNNKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDC4</accession>
    <name>A0ABQ5SDC4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="15917" checksum="3B9B55F0E5253F07" modified="2025-10-08" version="1" fragment="single">VGYGPALSALTLSAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVAEVNALGLSPESSDCPQVTLADGGKHPIVGRITLSLSLGPLRVVTRPYVLQGLAETAPYIMGSSTLRQYRARIDMETATLHMCKGTLSIKVPFLSFEHVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDF8</accession>
    <name>A0ABQ5SDF8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="375" mass="40544" checksum="07462BEF39AF10A3" modified="2025-10-08" version="1">MLLFASEGTLALQSGTRLAQRTGRVPALSVPRKWTKENTDRAKIAKYTRKGTLRLCTSYRTRANPDDLLLREEINASENSTTISTRKLSDGLLSREDNADTSLPVVPRVVPEGLLSREEESYSESRAPNLIRIISAGLLLHEDNSTAASGASVAGRDVPEGLLSREEGSDSETSNVRGRDVPEGLLSREEGSDSETSNVRGRDVPEGLLSREEGSDSETSNVRGRDVPEGLLSREEGSDSETSNVRGRDVPEGLLSREEGSDSETSNVRGRDVPEGLLSREEGSDSETSNVRGRDVPEGLLSREEGSDSETSNVRGRDVPEGLLSREEGSDSETSNVRGRDVPEGLLSREEGSDMDARRSAVDSTGCSSGARWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDU8</accession>
    <name>A0ABQ5SDU8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018971">
        <property type="entry name" value="DUF1997"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34131">
        <property type="entry name" value="(RAP ANNOTATION RELEASE2) GALACTOSE-BINDING LIKE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34131:SF3">
        <property type="entry name" value="(RAP ANNOTATION RELEASE2) GALACTOSE-BINDING LIKE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09366">
        <property type="entry name" value="DUF1997"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="281" mass="30577" checksum="B7F71495C4D17CD9" modified="2025-10-08" version="1">MLFTKHRRLMVGGAGGRERNLAVGAPCSVSPRLETFFKKTRTKDDLIDPAQIGATGSASAKFQILPQYESLQSYMLLPVEQYFVLDPTQISHLDGNRFVLTVPRINLFNIWLEAVVEVSVISYPGSKRAGPRVVLQAENCRISGSQVVESLRLDQRFAMHFVTELTWSATPVAAPVPAAAAVAATATVGPAPGTAANASTLGVMNGEIQASSQLTVWSEVVPPFHLLPRDVLVTTCNGVLRGLVSSLLPLFVRMLAQDYQRWAKDPAYRAERATRGKTFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE92</accession>
    <name>A0ABQ5SE92_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="395" mass="40805" checksum="D4FECCBFAEBC6F0D" modified="2025-10-08" version="1" fragment="single">MPTLEAGGDVATTITTTTTTTITSATTEVVEVDALVDELPAGTPATTAAAVVETISNDNALPSPPPLSATAAALDAAAAGATLTSLRLLQRIQTALVATLRGSRRVAPALAVKAPQLACHPALFLAGYAADRAVAGAQPPPLPPASGRGHRSQRQKQQPQQIRAQEHYAEKEVQQESLRVLMAEASKLAATTSSVVVLQLLQHGGSRVALATAAHLGHAVLQPHPELHPQLLLPLVHLLAFSTWAERVDARGLLSRRFEPHQALPTRAALCAWLERLNGPHKGPFLELLMCALLRAACGCRRGALPPGLIAVSMAEQDTADDAAVQTGTGTLSHNGSGEPAAGDAAVPRGPSTSCGEVTAAAHCPVWLDSGTATLVTERPGKSPQPRPGRPGPCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SED5</accession>
    <name>A0ABQ5SED5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="363" mass="39344" checksum="6CDA322C5A2C024F" modified="2025-10-08" version="1" fragment="single">MQQGCNWHVAQAICSLFDSARDADCEIVFYLEKNVWLGAETSTVLPRPGSDGVAAPACTRILLGEPLPAHQFVLRYASERFGAQLARWSQQEENNDILQCTTAITDVTAHHGQPSCIEILQEQQSNMIFAMMLDQQSVASAEEDAAPFSPPTPPEQQQRPSMMAKQVAFSTDETLLASRKESHSRPADLECTPASESTPALARPSTIKRLGNSAEPSRLRIVSSGGPPKLFIPLGSADEVPSARAAIRFAYSGEVVAGSIREVLEMRRQGAYLQITGCTAACDDVIQRRLITDAMGKLAELSGCEAPSVHSSTSSGAQSSAVLELFVCEALWPDLEEEPSFADMAEMAKRLLVAHFRTSLHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SES3</accession>
    <name>A0ABQ5SES3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="480" mass="49714" checksum="BA56342DFDA13BD4" modified="2025-10-08" version="1">MPHALQPSPGEGLALDPLAGSGPASHFNLVTSCAPPLVFGSRLPEDLNILGSEISESSTMPAVSDPSGASVSALGTEAVLSCNAPYGDTDMPECCTYTPQQHTPVCASVLSALHLLMGANFGPPLQQQLFPIPGQPVFLVPEALPVLSSQLIQFLGGIPGLAAPSPIPSMPAWGMLHGIGSPTQFPYQGPAYVGSTGFWLHGMYFPSYEHYIAYRRRTIAYNFHFFGIRPYFRPPRGLPRPRPPTSGAKSSVDASAHSAVAGGALPPASSGYDTLIPGSHAGKACEDASASPCGSPGSSSTCSNGDSDDIGSDISHVDPDTDVACGMQKVAARLNCRHLSGSRLKAAAEAGCPGQRCGHQTQNGTTIPAAGSLSPAVIDSSCACASNVEQRLMSSCAGCAPVTLPCALSPIRCFNSAHSNNSVVNSPRDEVLEDVRLANMFGPAWSSAVAGICPQWLELHFRVLVEKVVGDAILSVEDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFY1</accession>
    <name>A0ABQ5SFY1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="542"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="571" mass="58670" checksum="1C1C1AF5511B1194" modified="2025-10-08" version="1">MEGGAKTAPLLGAVPLAHTAIMAGTAAAARVVPPAVAAHSAYGGTRAVVRGTRHAARVGGPPLSWPFNMADDLLEYALHVAGRIWNTWLALVLLTAILAVRIVGRTTRALAGAMAAAAEGPAKRMVHHRQVARDRLLGSGYDPGSRGVFMAVPGPVMHLMLPAQVGANLLSSGSAHGGEHSGAGLLSYILAPAVMAVASSVAAADVWAWRIRRLFVGVSHIFPGSRTKGLSSLPIRAGEEHVGVDVSGGAACAPSSYPPTASIAAPVAKPASASAATAPEPVGAFRLIPPIPVAYPGIGENRGITAATIATGTDDDPFTAPADAFAAALDLMGTATRGGLGNGEDSPQHRRSRAHDLMPYDLVQGMDYGADAEGCEDVPNELQPATSMDKDRDRQRSINLDALDTGTAVGIRSRHEPHHHHRQHEHDMSVRWEGLKKSSVGSGGGDGSSDAPLQLRRSEVVSNSGALTPPDAATGPSDRQYPAGTAAAADVEDDGSSDFGVRGRFFGYTPMYSGLNDGLGAAAAPQSQREDLHSETHGSVGGALASLGGKLSCSGCGDVYRDPRDEKEGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGD1</accession>
    <name>A0ABQ5SGD1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="397"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="47881" checksum="2250B0612DAD013F" modified="2025-10-08" version="1">MGLLQLGSLVLIGGPLMAQAGLLATITVYSVGHFFNDINGASFDSCLQTFIYQNKLPIKFTDRAVVRRAWTTSAPNSGLAKSILDYSIVFLGADMQANLATFTNMLQDRLLWYDLLDAAQAGCSAEALYRSPSTSTNSSSPRVEPLNQRILAICTSNSIATVLAPTAGSPCTFFLPQQVCSPPPPIPAPPRPPAMPGIPLAPQPRPLPRPGLPPGMGTCTLIIKALRPTAWPPASACSQLLSAVGALCTFNVQMQTGRDWMCFDDGSKNGQLIIVGTAASRGDAQQLAANFAEHQVASAIIILLGGMRCGSVLQLDGSSCGVRARYDSAEQPELFSCFLPPPLPPRPLPVPPSPSPQPPLPPAPPSPALPLLLLPKPSRPPLPEPPTLSSPQPTPPKSLVVPMPMRLTRPLPPIPQGITGLSQAKPQAASFKERPPPSTKRWPPSPPRKQPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGG5</accession>
    <name>A0ABQ5SGG5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="125" mass="12189" checksum="8E82D58234EDBAE4" modified="2025-10-08" version="1" fragment="single">PVAATRLPSEMLPRTPRPSLDESYVPIAGSSGDSGGGAAAGTNDQSGPMELMAGAEAGRTAGGGNGAAAVPTTDAAAACVWPEGMGLADAAAAEELAMAPVLLSGEAAVVPHHQPQLQPPYGDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGN4</accession>
    <name>A0ABQ5SGN4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA replication licensing factor MCM6</fullName>
            <ecNumber evidence="13">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_013636</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="13"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17757">
        <property type="entry name" value="MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008049">
        <property type="entry name" value="MCM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041024">
        <property type="entry name" value="Mcm6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF43">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18263">
        <property type="entry name" value="WHD_MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01662">
        <property type="entry name" value="MCMPROTEIN6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="15">
        <location>
            <begin position="343"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="664"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="675"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="709"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="729"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI69091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="892" mass="98547" checksum="7BDCB0904614FEDE" modified="2025-10-08" version="1">MSRQDAAFADAVDWTPELVETHFLGFLDTFKDTDDDLEPHYLNALHELKAHDKGTLYVHIGHLNAYDQGLCSYTIAQFNRIEPTLRKALHAFIRTHEPGLVDGTDSREYYVALVSAWPQRMRDLRTSKLGQLTAFAGTVTRSSEVRPELLFGAFKCMECNTVIRGVPQQFKYSPPVLCTNPTCGNKSHWSLIREQSVFCDWQRLKVQEAVEEVPAGSLPRTLDVIMRHEAVETAKAGDKMVFTGQLVVVPDVSALAAPGEKVQLKEGGNRRDGADGVLGLGKGAGGRELTYRVMFLACAAQPADVTKGMVNIRPDVEESSEAIISEYHDGGESMLAMARDPHIYQQLTRSICPSVFGHDNIKQAVLLMLFGGVHKKTSEGINLRGDINVAIVGDPSCAKSQILKYVANFLPRAVYTSGKASSAAGLTASVVREPENNEFAIEAGALMLADNGICCIDEFDKMDVKDQVAIHEAMEQQTISIAKAGIQATLNARASILAAANPMGGRYDKSKPLKYNVALPPAILSRFDLLHVMVDDTTEATDARIATHIINVHRYQQNAFDVPYDTESLQHYIRYARSIKPEITPEARVELVRSYKELRTDDAAPGTQSSYRITVRQLEALIRLSEAMARVYCDKEIKPQYVREAKRLLRASILKIEQSDTLLEDDMPLPGLQRQPEKAPEAQIERGEAPTEEGPYGNDENMPPGVAVGDKRGADAMAEDRQPKRMRTYPSSGTSANQSESGEGATAPAPVTKEPVRVSAQKLNYVKSMLAKKMVEVHNQYLRQLPAYKPGESAETSDDGAPLPESGIRQEELLQWYLDTEALKGKVPNQAAALQELEVVSKIINHLIRKGDTLAVLSRPARQPGEDDASYLQRMQAERVLQLHDNYAPEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHH3</accession>
    <name>A0ABQ5SHH3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Formamidopyrimidine-DNA glycosylase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_013258</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.</text>
            <dbReference type="EC" id="3.2.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08972">
        <property type="entry name" value="PF_Nei_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="entry name" value="MutM-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049332">
        <property type="entry name" value="Fpg-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="FPG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="H2TH_FPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22993">
        <property type="entry name" value="FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22993:SF9">
        <property type="entry name" value="FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21218">
        <property type="entry name" value="Fpg-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00898">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="N-terminal domain of MutM-like DNA repair proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formamidopyrimidine-DNA glycosylase catalytic" evidence="11">
        <location>
            <begin position="43"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="330"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="421"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="447"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI68767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="505" mass="54792" checksum="5EDFDC71170EADE1" modified="2025-10-08" version="1" fragment="single">LFTKLVLDFRKYQVPGSAVLWSVLTLTLTAIPHSALAQIQAMPELPEIEAAKRLLERGCVGKRIEKAVVANDDKVFEGASPDDIGRALEGRRVVAAHRKGKYLWLELDNPEGPWPLLHFGMTGGIVVQKMAVTKYKRIHLESDPEDWPPRFTKLELDLEGGAKLAFVDARRFARVKLLVDPPSQEPLSKLGFDVLNELPSVEQFAAAVRKRVARAPGLKIKALLLDQEFCSGIGNWVGDEVLYQARIHPEQTAASLDDTALAALHSAICSVVGLAVEVEADSDRFPRDWIFHYRWTNKKPSSVNGHKIEFVTVGSRTSAFVPALQKLKVNGDVSDGVDGGNRKARAKTKAGGASGQSKRGRGSSKKAASSESEEEEEEEEEEEEEEEEEEEEEKRDSDDGGAKAIAAKHSRKAAAKPAGVGKEKGAKADRKGSKKAGGNKAAGFGTECEEDEEEEEDSEVEETPKKKRPRATKTAAPAVKKASKGPAAKGGPMQSAGPRGRKAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIK0</accession>
    <name>A0ABQ5SIK0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DEAD/DEAH box helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014406</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="220"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="463"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="13"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="517"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="526"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="691" mass="71879" checksum="7593FAEB4602BAC8" modified="2025-10-08" version="1" fragment="single">MVVQDLAAGFRSWPGHRRSAAPAVRPAGQWPQPAPTSLRSALHTTSPGCRRVPPIPRTTCSTGGLPVSFHQHSRTAASATRDNSGDASSGTSDSRRGGSGEGGDAEGNPWGDGGNLYGYLYDPDSQPLGPGGRSASLSALVANLSAIDDTNALQTALAAAIQSEDFELAAAVRDQLRRVAGGGAAAGRIDWAATGMPSWLADRAQRLGYRFPTEIQRRSAPALLAGSDAVVASETGSGKTLSFLLPLLARMSYPPDIFMDEMKGPQGLVVVPTMELGVQVALTAFRLLGGNVSAGRPGDAANMFTYFGPKGIKVRGILNKEEVVMAKSSLTYTAQVHLLVATPSALREAVEEPQPARELLRHLKVLAVDEVDECFSAFPADMDELMRLAADPGGRGNSLGTKPQVIFVGATQRQDVLQAAVSRGLLREPVSIQVGREGTVPTALSHRYIVVPDARRLAALARSLRADLAAADQDAAPARVMLFTNSPAEAAAVAEPLRSSLWSDHRMAVLVPPGTILGQDAASEEPPGPASSPSAAPPTPNRGGNSGARGSISSRVGPAPSASDGGAGPGAQSIDDLFAYNPIRALHSFRDNKSTLLLATGAAARGLDLPGVSHVYSLGPPPDATQYLHRAGRAGRIGSTSGGVVTTLVTPEELPLLLEIGRQLGIRLVEEEEASGEVVGMLPRLQAPPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIW8</accession>
    <name>A0ABQ5SIW8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013979</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="2">
        <location>
            <begin position="136"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="541"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="624" mass="67884" checksum="E506BBCD1AB864F8" modified="2025-10-08" version="1">MACDVPSLRRRRSWQDRGRRSKWNVMLLLLAVPLAGNGIAAAMVRSSTSRVQGRLVYRAAHTRDIWKLLAPSGVAYKLPSQPLDSIGDQIPSGSTIALDCTSDMRSDGTECLSISNAEVTRAAAPVKSTDLVLQLLVIVVSLTGSCTRAAVPVDQVRQAYTSASGYINFLRNCSYGKVAYSTEPGGVTVIETTIPCNQAILMCEEDAVAYGAQVAVAQQHGVNFLGSFNRFSYVLPKGISACFWVGLADLPGMNTWYSPTDDGIFNKGTVLQESLHNFGIYHGWRGSFEYADTSTAMGSGNSCPSAPELWRLGWASVLELLDNSTLPAGVIKGYTLPATSLGPTKNMLKIRPEWLGQSYTMNVYFALRLRMGGDQDISDEFNRKISVHYLDKSIDNNFGVRGDPKVTILGTIASRSTTNLEDYKLLVETGDFDEDGSKIPLWVCRYEVRAQECVVGIGPKSSPATIEVLASSQAADYDSYRDASPSPPRSVRPPPVSRQSPRQPPPQPPPQPPPRPPPRPPPRSPPRPSPSSPLLLPAPSPRVQSFPRSQSPWPPLPSQRPPFKLRQPPRPLPSPPNPQQTSSSPRAPVRPSKPRFQPNSTAPPLQPKPGRASHPLPSEQRGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIY9</accession>
    <name>A0ABQ5SIY9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_014264</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045395254" description="Guanylate cyclase domain-containing protein" evidence="9">
        <location>
            <begin position="24"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="10">
        <location>
            <begin position="1174"/>
            <end position="1306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI69601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1388" mass="149985" checksum="837ACE79B865BDE3" modified="2025-10-08" version="1">MNKMLLCLVPLSGLLLAACLTAAQQTLTFHIGATVGRDGSCVPFYRGTALGYQYFFDWLAKRAPIAVTDASGKTYLLRLQLRLISHNCTEEGRAAAMSSLINGTEFEPPVHLLLGGNDESALEDALQAEAASRILMHCCTARDTVFHLGLSHVYGLFTSPSMYTGTLLRTMALRGMQRVAVLYNRDSLLQVELCEGALTQLPTLAELRPEFRAVLIANYSALDADSPGFWGKMGARVASAGADVLVACDSMNRTSLLVHQLRRSGYRLSALWLASSGNVSEFLDYLGGHREYALTTVQWMPSLPYADPDFGTAAQYYAAFTAAMGQQPSYFSAAATASGYVLLTALQRVISTCSLEKARANVNIVDTFMWSEGALSCVDKFQNTLDIKGKSGTDVLRNVLSTLNMDTFYGPIGFNGFHQNIYHPVIGAQVLSGSLVAVLPLDVADKALVMPIPVDPPKAKSWITTPTGISVVTLLFCIGAACMALLFLVCRRRMGHHLHAHSVELLLDDIQVDIQPHINSDGSCEPGEGMYRGTRVKLVVANELLLWSRSQRAWKPDNQGSPLTSVFESSECSGQRQLAAPLRSYALPVAAETRSPAQRSKSAALEDTCVDRTSQGSMRLTGSEGFGLFKQQSERFIRNQIDNVTPRTTVGLVSPANCPGMQNIRNQPVTMPGLLSRRARRQILALVWRAVRLQHPRMCPVLGIVWEWPGLVENGGKVPVVVRQWHELDSLERLLENETVPLSLMTKATIAQGVAEALAYLHAQGPPIIAGPLEASRIIMDKNFNPHLFLRLQNLDPEYINAAMASADGSRMGKMRALSVFGARPLQLQQSATVSRAAPTAQPLKAVPSAASDPTTSTAVLHFTVTMPMDDDGSRVHPQLQGIGVGPYQQQQSRITSTSVLSDVEQPAMGTLRPAGLFASQPRYRPSKEQDVYEFGLLLCRIFVVASPEDSGRLRFANIASAAIAPVTATGGDGGGFDCDTSVAGVSAFLSTAAPPVPILQTVDPEQLDMVISEVRGICPELGNLAWACTQPNSGLTFPTIAKALEQVVIPALTGSDGLPRGLRRNALSMKVYGSAGSGGFGGGSAGCGSAPRQEQDLELRSMSSGVGRSGPSQLMPRRSGSVTWPDHLRAVRVNHHHQVVASDDLIFDIFPPKVARALQAGQTVQPERYDCVSIFFSDVVGYTDLCGQLQPGEVMDLVHRLYSRFDDLIRELRLFKVETVGDAYLAVANLRWPQPDDHARLMAQFALFAVRAANSLLVHPERPELGTVHVRVGLHCGPVVGSVVGTLNRRYCLFGDAVNTAARMEHNSMADRINCSAAFAALLREQWPGGARIVSRGILAVKGKGPMETFWVEQEQTTETVLALDDVTAVAPPVASGALIPDAVEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJH4</accession>
    <name>A0ABQ5SJH4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_014523</name>
        <name evidence="9" type="ORF">VaNZ11_014545</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="8 10" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000088">
        <property type="protein sequence ID" value="GLI69819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="32"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="391" mass="41905" checksum="82F80730062EA88F" modified="2025-10-08" version="1">MRICPLIQIRTIRRCPCAGSLKRASVLRRLAQGPDAPGPVSKTDPRSPQPQQQQQQQQQQQQQSEPQSGVSPETLTTRIAKLVAEVVASPLFYLVAGVLAIKLVASTGEDGATIFVFAALPITALTALSKSSVGKQVQQQLEAKLPELQAGAERQRLAHQQARERSKWYGTGRPRLPGPLGSAEHLMGQTAGDAGFDPLGLAREPAAFSRYREAELLHARWAMLGVVGCVVPELLALRGVDLGEPVWWKVGASKLSSDLTLNWGGIEGFRIAGKQGIGLIAACQAVLMGGPEYARYVGIRSLEPVGVFLPGEQNYPGGGPFDPLNYAADADGFVDQAVKEIKNGRLAMLAMLGFFAQAAVTRQGPMQNVLDFIADPAGNNIFTNVTRLLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJL1</accession>
    <name>A0ABQ5SJL1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1163"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1212"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1232"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1250"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1278"/>
            <end position="1287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1287" mass="137888" checksum="285C0487B4253CF9" modified="2025-10-08" version="1">MNIGRKFVPFLESRIKMSAFCPHSSCGKLKSISSLHSASQALALCHSKQQRLLGVLGLCLLRRRATRTVRCGNVVPNTNAVHSKGGFKVAAVSGLDGFRTAGLDESGDLQISADGGSSDQLSGREQGNQRHSLDKEFLQLASKPQPRARARPSHTQSTVGDSSPDSDCFGRRDADDVTVSRQEGVEGVAEDMQCCSSSLNLQHEPGPMGLKAKTITVRRKIIERGFNLLPEQSLSAPLLPRVGNLMPSTGGRVAQRRLAQLNKATKRILRVSRNASTEASSGDDADPDATAASAPSTASTLLGTAGRQSYGHPPLSSVNSDVAVPPDTPTQLSIASSQALSPSALGYRRASASPSIASPAVGLAASAEAVSTTSSTYYELPIPGSMPRPTRAEVERLARECNDLGALRILYGMYGVRLAREELVLLVNQLAEVHDPSYMTVKLWTATQAMIVELLQRITPMLKELTMQEYVMMLAGVAKLRYVPPSDWIAAVLTQSKPRLYNASPSHLAHVLKAIARISVDPSDVKSAATWEGWMRRFLTAVQRQLPTFRADEMVKTLVALSELRYRPPPEWMHKFSAALHSRLDVLEAHSLSEVMLAFAALRYQPEGPFLRAYYSQLYARLPLADDQDLATYAQAAALLDRVIRQDFMQEFLAEVVEKLPTFKPQHLANLLNAMGRLATPTTPDRVRVPPGWASAVADHVGANLSGFSGSALASAAWGLSMLGHKARPQFLDDLLSASYGKLHTCGPTDLILLVTALGNFGFEPMPGREALWATWTVEFERFAGRKVYDTRGVCDLMDAFARVPRPLVPAPGGAGLRPRPLHPDFVAGVLRSTRIYQLRNTSPSRLAALAGALATLGVRPDFGFLYNYAQAARVMWTSFNTRDYATLLGAMVHIGTPGLADSRWAADFLAITLCRISEFDGPSLTASLDALRRLPKPEFTPSNQWLAAAANRAVELIRSPPPPPPPVAVAATELAPVPAAAMAAVSATVAPLPVLAPHQLPGTVSGVSSASSSGKPIASADWWVLPMRPLTPELLVVLVEALAALGYRTEPKAPLVQIVRNLLSTTVARLQLQERVSTGAVATIPPTAGEIGALQHLHRQELFHQQQNGSMAVAAAAFERAKAAAVTAAAVAAGEVPRPPLDRQQAAALEVTLGILLRLPLPQEPPPAPVAPASSGSMMPDLPRTAIRTAEDLAAADASFATKRVLRAQKRANRRPAQELDELVNPATANSAARGRSSSSDNPTGNSGDENREGSRSKLDTHGVVGRPGVRMPLYSPESSGRSPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJM9</accession>
    <name>A0ABQ5SJM9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Plastid/chloroplast ribosomal protein L34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014627</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00391">
        <property type="entry name" value="Ribosomal_bL34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000271">
        <property type="entry name" value="Ribosomal_bL34"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01030">
        <property type="entry name" value="rpmH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00468">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="13758" checksum="A521DFC01233D827" modified="2025-10-08" version="1">MASLMAARGAFVARTAAPIKCMASMRSTMTSRAIMPIAPRTAQTSSFLGGSFAGSSLVRVAPTRGGLLVMANNKKSIACTKEGTNRKRRRTSGFKARMATKNGRKVIKSRRAKGRHVLCPASEGKSGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK03</accession>
    <name>A0ABQ5SK03_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">argininosuccinate synthase</fullName>
            <ecNumber evidence="2">6.3.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_014386</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="ASS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="entry name" value="Argininosuccinate synthetase, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.470">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048268">
        <property type="entry name" value="Arginosuc_syn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048267">
        <property type="entry name" value="Arginosuc_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001770">
        <property type="entry name" value="PRK00509.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587:SF2">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20979">
        <property type="entry name" value="Arginosuc_syn_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="Argininosuccinate synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginosuccinate synthase-like N-terminal" evidence="8">
        <location>
            <begin position="41"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Arginosuccinate synthase C-terminal" evidence="9">
        <location>
            <begin position="213"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI69697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="441" mass="48807" checksum="12D556F092B4D265" modified="2025-10-08" version="1">MLGLKLNQRCVSSRAKPASGPVRGRKACVSVRAAASGKVKKVVLAYSGGLDTSIILKWLQDTYGCEVVTFTADLGQGEELEPARAKAEKMGVKQIFIDDLREEFVRDYVFPMFRANALYEGTYLLGTSIARPLIAKRQIEIAKEVGADAVSHGATGKGNDQVRFEVGYYSLKPDIKVIAPWREWDLLSRTKLIEYAEKNDIPVPQKKRGEPPYSMDANLLHISYEGNALEDPWVAPEESMFTRSVSPEQAPDQPTTIELAFEKGNPVAIDGVAMSPATLLTKLNELGGANGVGRVDLVESRFVGMKSRGVYETPGGTILQVAHRAIESITLDRAELHMKDDLMPRYAELVYNGFWFSPEREALQALIDKTQEFCTGVVRVKLYKGNVQVCGRKSPYSLYDKVIASFEDDKGLYNQADAGGFIKLQALRLRTLGVNRYKLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKI8</accession>
    <name>A0ABQ5SKI8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014667</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="34079" checksum="8AA8EE0C3777B579" modified="2025-10-08" version="1" fragment="single">MSEQLAIYRRCRRQYPGTAACVLLPAFCRRKAEASLRGMRHVHRFEPGTVLFNGVDWEGTLAPWPHLSYSVDVWYEAPDGGAVGYGPALSALTLSAGHSLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVAEVNALGLSPESSDCPQVTLADGGKHPIVGRITFSLSLGPLRVVTRPYVLQGLAETAPYIMGSSTLRQYRARIDMETATLHMCKGTLSIKVPFLLFEHVTPKEGETEAVPMVNFAVTAMAARIEPELVGRKEAVRLLRKGAHGLLLRPRVSCCVTTAQISADPEVEALLSQYEDVFKDIPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKK4</accession>
    <name>A0ABQ5SKK4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014689</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056508">
        <property type="entry name" value="HPAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044845">
        <property type="entry name" value="HPAT/SRGT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31485:SF4">
        <property type="entry name" value="HYDROXYPROLINE O-ARABINOSYLTRANSFERASE RDN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31485">
        <property type="entry name" value="PEPTIDYL SERINE ALPHA-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23452">
        <property type="entry name" value="HPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14295">
        <property type="entry name" value="PAN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Apple" evidence="7">
        <location>
            <begin position="116"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxyproline O-arabinosyltransferase-like" evidence="8">
        <location>
            <begin position="222"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="62380" checksum="55D4AD56AC140D1C" modified="2025-10-08" version="1">MASSTRVIVVAVAFTVVATFYSLSELHRMSALHASSETKTFVTTGDNRVLLGGTTQDTVTSSPSRVTVSTGLAGTTAATSPVASATTTVGGSAFTTAASSSGGSASQHKCFTYEHTELWGDVVVWGTVHKKSNTGECCDACRSHQPQGDEPGCNVWVFCGDQQRCGAQYQQCWLKHLPHPEASKPAKQGPNVYWTSGTIDVDINADPGAQLQVQTSGAPRMFHTITSAQGAAVHWQVRIHYYWWKKRKAECGKTGKCEMGGFTRLLHSGAADDLMDELPTVVVDPLPQSMVEHSWYVVLNRPYAFVQWTQKVKIPEKYVLMSEPDHVFLRPMPNFMRNDAPAAFPFFYIEPAKAENAHITRKFTGNISQRQLEEIAPIGNSPTFMTFDDMKRVMPTWMNVSIAVFKDQEANSVWGWVQEMYGFTIALWLNGIKHVDLFLHMMAQPPWDQEMQMANGKPFYILHYTYGMDYKLTGEFTPGKFGEWRFDKRTYGSRPPPRHLGDPPKNMKNELVRALINSINEATAALPCWDTYAESGKASYDCDEMRTLADPRFKTDGKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKN8</accession>
    <name>A0ABQ5SKN8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014973</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000091">
        <property type="protein sequence ID" value="GLI70186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="20119" checksum="CF18AE7B471FF257" modified="2025-10-08" version="1">MMAGRDDPSFVGLACQPEPDTVPRMFDRDSETIDTITAQDPLVFQAPIHLTTPHLAALTVTDGIADQRGLLSPQMQDRANGNEVKWFTIPAPKLFTLHDMNVERDIVNATHFYLRDIWKYLQLVRGGDHDEVRCLFVGNTLEGIARTWYDQWITARENITFDELIVALLAHFIPQVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKQ3</accession>
    <name>A0ABQ5SKQ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015013</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF58">
        <property type="entry name" value="RECEPTOR PROTEIN KINASE-LIKE PROTEIN ZAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045630709" description="Guanylate cyclase domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="2628"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="4">
        <location>
            <begin position="607"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="743"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="808"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1176"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1657"/>
            <end position="1678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1760"/>
            <end position="1800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2161"/>
            <end position="2211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2235"/>
            <end position="2263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2578"/>
            <end position="2628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="819"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1192"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1230"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2168"/>
            <end position="2201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2578"/>
            <end position="2588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="2589"/>
            <end position="2607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2628" mass="271696" checksum="58AB4480EA5FFEE6" modified="2025-10-08" version="1">MQKLCLFLVLTACVTDCASRSDELALRHKGNAAKAVWKEDFTLKGVSTHKVGLRQQALRRLQQNADIATRSCDSACQKDQRNALSKLFEVWGGSTWTQGDKWLSNSTVCNWYGVLCCLIQPVGPVGAQDLMLFNTEQSDCPPEATGVLGLSLSSNNMTGNITDVHWAAFSSTLLLLDLDDNHLKGDITTLSSGGPSEAGGLALLTSLQYFSAGGNELHGSLDLFLALRNLSALILHANNLSGTLPEGLMMHPSLEFLDLDNNQLSGSLPNITFTSSNHQRSISLSSNHLSGAFPEFPKTSAAAAESLTHLDVSNNRLDGHLPPYLAWLLLDYLDVSDNRLSGPVAHLLQTSWALLMLDLSNNSFNGTLPSDVRCRSVHSLDLSHNNLTGTIPASFANLHSVVHLLLNDNPRLNGQLPDKLPTLTQLSYFDIRNTSMQAEKPGAAGLPQLVTLSESTFIISSDPDYPTCPTPLLRGSAPVGIAPVYWQFDGCTCKEGYTGSCTRGLPPGAADGNSSGGNDGGNSCSSGSGTGDPIIGMSCVPVHGVSEQAPVMPGWGIALAVLGGLMMLTLLAILAYHLVPSVVRYRTMLAKRMPPGFSRRAGGCVVTLVLTDVEGSTELWEWDTDLMAAAIDLHDHTLRSQMSKWYGYEVQTEGDAFLMAFHEPLDAAGWCITTQLALLNADWNRELFRHHKACIETLESLQPISQTHSCRISQTGAGAATGNAPHVTEAGLLTARPFLPPGIILGPGPQPTSAVGGPSELSSARGGLTPQRISVGGNDDASAAAAGSCIVGVGGGVSGGLGGRDGAAAASGPGGGCLQDNNRQSKLSSEVKNHDATLGGMANAAEFADWTRYTRLGVLYRGLRVRMGVATGVTDAVTSHAITQRMEYSGEVYRRVQAVADLPHGGQVLLDANTFNAIHNNLNDLGIRAVQILTRDKRGSNSGTRAAFGGVRVGDRRRVARSIRNRQSLDLAYAKEAMEPPGLAAAAAAADGDEEVCPGGAGAPSALSIATSLRALSVGGSWMRGAVGATNIATPINSLRSALGSGMGYVSSTFARGSSPLPRQPGEAEPSGRSFRMGFISFGRTSANHQNHLVHSNYPNHYINNANSNYNSNSNNDSNKLQLPGFQAAAEAAAAAAGGEVRSGLLSGQSAAVEPCGIGGGSSYGPNVGRGLLVNRSGSGGSTGGRALQGLVAGGGGGGGGGNSGGVPGPVLIPRQRAPSATGTYGGLTRHLERVSQESSCKDRDEVPDPDLQLVDGPNRDDREVGSGSGVAQPQASPTGRRRSGGDGGGGGGGGLAAALIVGDVPMSTSDIDSVHYSGSPRDLRICHLRDVPTGPVSQAAPATRATAALNTLAREVIQRRSSMAPAISVVNMGVYELNAGCSLEYVNITQVLVPGLEERARLGKPLDSARQLTPGYFNAPATSVAPLGSGLWAGLRQRGIFPLVTLVFCSMERFTEMAAVNRDLSMNVMAAYNDCVRRSLLACNGYECQEQEGNYMIAFAKPSEALEWCLMLQELMMEVGWTSAMLRLPAMQEELHPLTGTVLFRGPRIKAGVYQGMPTRVSPHSTTGRADYFGPLVNRAARYCHAAAQGGQVIVSRCLVEEILRELLQGGLLPAEELPTEELPVRQVAVIDNSGVVLPRWCMPQDVMGSGLGASGGGTATAAAPGSTTAGGSGGNASGFGQRSGAFSKLVVPLVDASSAGENELAASTTGSLMVLPEFPGSTYGVSGGVGGVGVGVGGGVGGDGVGGGVTRLRRVRYQLPSVSRRRLPPRSSNGGGSGTGESDRGGADGVLVTSTQPGGSAVPALHHALTFEPNGVAAALDASACSSASLTSGSASAAAGATAAAAVSIGATLDARGSNGSAVPRGGFTTIEVPLSELAGYGISPANVPRRMRAAVEQALAETRCRVASQQREHSQNQSRLGAPYTAPLLPSRPASRRMMGVPQSPATAATVGGGAAAAPVGPIPYRSSLCGVAGSSGKLLVGGGGAQSVCVTGGGMAAATGSAIAAAASLPLQPQVLGGPLSHSQAGDLPPPQQQYHLPGAHVLPQQQQQQQRVGMRGLEPSHLELTPQFQHDLHQQLLVLDSRGSTPGGGGGGRPVDPLAAAAADALTRPVPPLPIRVPVSQRASFDLHNNYMLQHQQQPVPQLTLALQARISAGPGLASANGRPSPSRSPFPRSAAYQLQQGSTQQPSSSLQQQHPQPLPTPPQQQPTLRAHFRAASGCVAPWGLAPSRSSFGRRGQGPGPGLLLPQRQGSFSGQDTQPQRWRDSVMAATAGASATLQSVESMLSHEHSAAFHALWEMATDEGGLPSSSRAEADLQSAMVTGLVGFDAEPGLGLQPPRSAACGGREAGPVPGAPRLPAALRDGPSARARHRGSFMVMDSTPGCLPTLQELCLRRRVGPYKWLWAHELVVEDLGMFRFKGVAGKHSIMSVSTAGTSERKLGSRVKKSKGERVEPGRGLLYRIVLQPVEVSPPPDVGLVQAAYASEGSLPGPRYAPPIASAPAMPRMPYSCGTGPLEPDTQLSPLLPTMGSLGNSKPPNPMPAATTAAVAQPPAPSQSVMAARWHPAVSNSDVISNSGGVSNSGGVSNGGGVSNGGAGCRGGGPVAVDASLGLETNNSPGAVPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL20</accession>
    <name>A0ABQ5SL20_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015276</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="243" mass="25898" checksum="FB683AFF93C33006" modified="2025-10-08" version="1">MALMMKRSAVAARSAVSRKAVVTCVARQSWLPGSEIPKHLDSPAALAMAGNFGFDPLGLGKDPEALRWYQQAELVHSRTAMTAVAGILIPGLLTKAGALNVPEWYDAGKVAIESSFAPFGALLAVQLFLTGFVETKRWQDFKKPGSQAEKGSFLGFETSFAGTGDNGYPGGIFDPLGLAKDADKLADWKLKEIKNGRLAMVAFLGFTAQKFATGKGPIDNLAAHLADPWHVNYASNGVSVPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL73</accession>
    <name>A0ABQ5SL73_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit Tim21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014939</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TIM21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.320">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit Tim21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013261">
        <property type="entry name" value="Tim21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038552">
        <property type="entry name" value="Tim21_IMS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032:SF6">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08294">
        <property type="entry name" value="TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="290" mass="32328" checksum="38772E450027AE3B" modified="2025-10-08" version="1">MQSTRTRVPLLLNTAIAFQRDLAIWSNVIRASERGMATQGVFGGLSRNSQRQLALIGEVLRSVSPCQQRFTKSALASQTRLLWLAQHLTLNEVPSLQNLRHYAAATNAQEQSKQPKEQVYDSLELTEEKINAITDKIPQRPMGVVEGTSYTLIILAAFGVLAFVLYHVLTSLIFEPTAITCFNHTLERLKNDPRITVRIGGADDIRAWGTNAESRVARQQIPHHIYKDANGVEHVRIQFHIRGPSGNGVVNADMYKDAQGQWQYTYLVVDAYPASGSGTVPQRLVIVTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLL0</accession>
    <name>A0ABQ5SLL0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734:SF4">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="503" mass="55884" checksum="547B3A56CE8A30E6" modified="2025-10-08" version="1">MVEKVGVLIVGAGPTGLGAATRLQQHGHKDWLLIDAAPEAGGLACTDVTPEGFLFDMGGHVIFSHWNYFDELLDSALGSGPEAWNTLQRVSYVWIRDRWVAYPFQNNISALPKEDQIKCLTGLVEAKVTNAIAQGRPKNFDEWIVRVMGPGIADLFMRPYNFKVWAIPTHLMQCNWLGERVATVDVDRAITNVINNKEDAGWGPNAVFRFPTSGGTGAIWKGVAKLLPEERQRYGQKVVSIDKDAKVVTLENGHQYQYGALVSTIPLDLTLTWLGKEEWAKGLQHSSSHIIGVGIRGECPHGTKCWLYFPESNCPFYRCTVFSNYAKLNCPADDAKLPTLCLGDGSAPASSEPKEGPYWSLMFEVSESNYKPVNQENVTLGGTAGTWSDAVRDTLIGAINTQLMKPGDEVVSLYHRRIEHGYPTPSLGRDEVLEKALPWLQQFGIWSRGRFGSYKYEVANQDHSLMLGVECVDNILYGTKELTLAYPDIVNAKKNNELLYLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLQ7</accession>
    <name>A0ABQ5SLQ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Heme oxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015484</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002051">
        <property type="entry name" value="Haem_Oase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016053">
        <property type="entry name" value="Haem_Oase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10720">
        <property type="entry name" value="HEME OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10720:SF0">
        <property type="entry name" value="HEME OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01126">
        <property type="entry name" value="Heme_oxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00088">
        <property type="entry name" value="HAEMOXYGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="29004" checksum="DB2F9710D5BEF1CA" modified="2025-10-08" version="1">MADSETLSKRFNKAGKSIHSLSDSLVNAKLIALLTDKQLYAKALSRFYYVFASLERALDEGLKSGDKDVEIFKSFFNKRSIYRTAAFRADLENLLGADWEVACGHKSTPLKEYEHHLALLAATKPRLLLAHSYTQHLAITAGGQALRRIVRKHLGLPEGMGTSAFEFKEDSSNTLKTRFKSLFDEWGRSLSEEDVQQLISEHLKAFQYNNAIIRAFPIPSTAVARGVLRITPQPLLLVALALVVLLIGLFIRWQGTRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM51</accession>
    <name>A0ABQ5SM51_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="203" mass="22486" checksum="76662E0E09B1A2C8" modified="2025-10-08" version="1">MQFETSTYKQNETKCATVMPEGTNSLEGTENQQQDDSLLRAPSLHDRLAAERHVPRPVPSLGLEDAGTLDLSEVAAAAGLSPEEADRLWREAQDEVLAELKGEIIQRQASDHVSERESPTHVHHRYHRRHFHNDREGPIIGSEGALIKFARVAAAERHESVRQVLHSLRPAHVDIERLDSKEQRDEVEVSGDAAAAACEGLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMJ9</accession>
    <name>A0ABQ5SMJ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_015772</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16449">
        <property type="entry name" value="RING-HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050776">
        <property type="entry name" value="Ank_Repeat/CDKN_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201:SF16">
        <property type="entry name" value="ANKYRIN-1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="132"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="165"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="213"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="1333"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="472"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="525"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="601"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="630"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="856"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="972"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1099"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1210"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="545"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="608"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="630"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="697"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="972"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1106"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1219"/>
            <end position="1231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI70745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1395" mass="144407" checksum="087E450018ADBD17" modified="2025-10-08" version="1">MNSDLDTSPGQPHGAFTVKSQLPGCCIYFRLPFFTAVEEGDATEVLKFLREDALWLKVSEKKGKRTAWHIAAAKGHVEVLQALVARTREDDVRLIWNLPAPLSSRIRTVTTGRDAPGPDERVFALVNCRPTFGPTPMALAAQHDHPEAIKYLISVGADPWLGDNEGRNPAHYAARTGAIRALQVLLEEEKPLTSRASNRNLPGTRYVDSLDTFGFSPLHYATWANHKSVMMLLIGFDANIIARSLIPHDEYNNLPPGSSPLHIACFLGDLERVKLLLRAYYETAADLLPSQTAMLGTTERRRRARSHPDPRLILTRTQRLPFHIAARAGHRHLLDWLDPSVPLMFLFGGEDDVPGTSGEQGGLVNLVGVSRLTVIAAKALHQALLTSLDVAADDIRRYEEEAEARRRRREERRLAREAKAAAQSLKQGKEPPSADSGIRGRIRRLIQRASRDGGGGVGGGTAVATADAGGRTLSGATAGNASDGVPKAGWRPWRSRTRKGRRKLALSGQGDGVMAVAAAAAAGLDRNQSHGAVPRSPRRRRRRSTLGTAGRLASAASLGGTATTAAGADGATAASAVNANRDDGATAASAVNANRDDGAAGSRELEKMQSTQAGDSGVLNSSLRLRLFRSSGSQRSQQQQQQQQQAQAQAQATQQPLSSPGPDAGGTAADAQENPEATVAGTAVGGSGTPESSGHGSTDGGGGGGGGGGGGGLAASLTRTMQRLYRGSLRRQSSGATSSDLVQAQLNERLNRAGITIPEGPDVAVAYFIPGREPRYNMPTQILLPAGGAFNASLQSSGTAIQPASPTAAYPATRTAEDNSSFTSGAAAPAVAARDRGLVGSRPSSQSTSTLIAELLNGRRGSDGETSGSVSNNGGGDGVIAHLAGQVSVSGGGAASVSDDGAAASISAAATAAEATMCPFWVETNSNLDFQSQAPVSRQGVVDLPPIRSAGTSRMPSLRTSIRLPGAASITAESELESPAPSALLPPLSLPPTRRPPSDGVAASGTTDPSQLLEVVAVCLNPAEVNGAAGDGGGGNAGTTAIVATEGGDDAINTTSRRSLSSGIGRVGAGVLQLFNSLRDTLRGTSSRELVAVLPIARTSPAQSRSRAHRRNRRTRTPNSSARRGRKAEPSQAVAVVAEGPATIAGASPGGRDGTGPAASIDLVRSPNTSHHHGSLQSHPHGSLAARGVRVASGWPHARLSLYSRLSGSTHQDAAVPGPNTSSVNASSAGTITRRRMPFRQSPAPSGEGGTQHGQGTTTTTTPEANEDGVDDAACGVAEAPAATTAPPPMDSVPVMVPAAAALSSSFSSTSPTESEGDIDAAAAVEDGNEDVCPVCLDEAPNLLVQTCRHHLCMECARDLVKRNSLTPALCPYCRGIICGFKAQVAVGQAAEKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMT6</accession>
    <name>A0ABQ5SMT6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="505" mass="53518" checksum="D4DEE23D8AF06EF6" modified="2025-10-08" version="1" fragment="single">MRTWKNASPARLRCLAGSSHGFNFHADPYGFIERVCSFYSVAVSKSVSADTAASISSSQLTLLNHLGTLPSVARNWDTRTVEAELRNVDAASFAELELSRSEPTAVQRVLAKRHGVVLPVGASRLAASATLATVQPPSIRSLAVLYGVLEYDGDVPASDAAARQLVRRLALERPHSHPLDTDLSPVARSQVNELFNRLGYIGRLPYSYGSAQLMTVCLRTILADVDDEARLRPLPVLDPRQADLIRYLRSRRAAPGRTRGMPAVLDVDLSSHPPVSRLAAARNILQLLQNHELCATPTGTAVEALQALGHVGPVPPTTGETLMLASELRDRCFRPKPAHMAALHAAAATFSGAAGSAAAAAAAAAAEPDSLAHFKAMLHVLRRADTDAATPATRTAQRHLAELRRRTPASSHAAAAAVAPWSDGGAAAALGPCPSQLEVRAAIEDLPLSETLMERLFDLGWQGPMPATHGSALALEEYLVHHKNTAATSPSGASPGPSRNAHADT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMW2</accession>
    <name>A0ABQ5SMW2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015771</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14650" checksum="3DE1633F1673EF9A" modified="2025-10-08" version="1" fragment="single">GERTCKACYKRYLRHKHYGRVAPLPLCIGEEEGDACTADAVEQQSTAMTGNPEDMLGGYVGPHPGRGDFGVGVGMAVRRCYECGTTRTGGNSWKRHKLIDGEMTCSACYKRFMYKGHGSYQRQTPQVMYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SND9</accession>
    <name>A0ABQ5SND9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="25275" checksum="336204C30D8BE0C1" modified="2025-10-08" version="1" fragment="single">RDVEARNVLAAGSYHMRRDENVPAYQSRFEALVTPIADLTESERVFWFRRGLTKLLAKWCATDLVGAQIKSYDALVAFAKGAEIQLLAGEEEVKSGPRANAVQVQDPLGEAVALDQDLGATRPTPTAATRRSNGQRKARGGASSGVRGTVGAGPSSDPARNRKRGGAPSSAAAQRSDPNVKRTRTDDTFMPKWNCNRSEFERRKLANVCLRCAENHSTKDCPLMPPFVPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNG7</accession>
    <name>A0ABQ5SNG7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016792</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="202" mass="21288" checksum="C5521C4921CF8C4D" modified="2025-10-08" version="1">MTSVEPSTDTAAADARVDILPSGEALRVVSGWLGMSVEEASLEAGPSASLQTAMPNRRQQFLGLGAKYLPHHKAVETATALDLKLRKRLERGLAARRLQEEEAHEAGRGGALTCGPKPSPGALVGVQMGEGPGAKRRARHPGKRTPEVGQNDSSDEDEYKAPAMGSVAITTSSKVAREAALLYAPVDCGSGKRKKAKGRREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNJ9</accession>
    <name>A0ABQ5SNJ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-mRNA processing factor 4 (PRP4)-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016847</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.110">
        <property type="entry name" value="Pre-mRNA processing factor 4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036285">
        <property type="entry name" value="PRP4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846:SF0">
        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00500">
        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158230">
        <property type="entry name" value="PRP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="4">
        <location>
            <begin position="38"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="258"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="300"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="342"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="384"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="444" mass="48687" checksum="CB688DBA2E1BF603" modified="2025-10-08" version="1" fragment="single">MADVFALPESGNREREAHAAKLREFELRRKIRQVVVPTDDGKVRQLLRQLGEPVTLFGEREMERRERLKRIIAQRDGEVELDLPTTEQLLSEEVAVQKELFYTEGPDELRAARLALARFSLVRAKGRLLGAKRRREDPEALPVVSDTQATAAAAVRTIVQQSSELGDTRPISHCQFSPDGSSLAACGWGGALALWSVPGCTKRWTAPVPAEGPRLTGVAWHPAATHGSHSAAAVNLASAASDGVARLYNGRGELLRSLEGHTDRLARLGFHPLGRHLATASFDMTWRLWDIETGLSLLEQEGHSRAVYAVAFHPDGSLAGSAGLDAYGRIWDCRTGRSVLLLEGHVKQLLDIDFSPNGYYVVTGSEDHTAKVWDLRKRGCLYTIPAHTSLLSSVRWERTAGHYLLTAGYDCVAKIWNARNFSLLKVLAGHEGKIMGADVSPTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPE2</accession>
    <name>A0ABQ5SPE2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="841" mass="89022" checksum="325FE498FEC1E40E" modified="2025-10-08" version="1">MLKGFLLRPSIRLGGRRHACCRPRCRGRAASLSAPPSGSSTSALRESQHSSDSVARLGALPCAVLSCAPSAVDRQGSEHSLHGVGNNDSSSSDFQVWRFKVATYEEGQHLHGGVRTGTSPATSHDATATTAGNDAHAIATASTGTGAFSGVNVDVIASNSNGSGNNRTPPSPLSPDRGLRDHGDGSYSQEEPRKDNGSSSVGTRGPCVATSELPAGSDLFQRLASPEVMTYDSRDVKEIPRSETNSTASSSSSSTSNVTSGSSSSHSGGRQRRRQLRLPAERRWQQHGGKNVNPEAVRLGGRHPEARPMGRHRQTDDDNSGDEEGGERLGARSGSSSSSCIGSSASPSTSPSRSRDRTGLACGPASGDTDEFVHLAGASLEHSVGRRSRGGDRSLHTVPSWLEARRINQAIIEAGAWRQYRKILQLVECHVRDFDRINTATALHRLGSCGLQPGSWEAKRVLTDPRFSRLMETLAGDYLTSLTAREVSNCLWALAKLGYSPGRHCNAVADAGSANSHGGSSCGGRSDDSGSSSNRDGGTTGNGGSCSSPGLLEGLCARVEMCLASLGPQEVANSLWALGTLGHHPGEHLLRGLTNRLLEVLPRAQPQEVSSALLGVAKLEWNPGPCVLDRVAHGSLTQIRKFNTQELSNTLWSLARLGHYNEHLQAAIFHQAVRRVSDFKPPELSSLALAAATMGLGPRGGEVGDSAREGGSYWRDGAGASSRVGGRAGSTGIPHQYFQKGLMPEQLLRTLCDQAAATVQVLKARATPNSPSSLHSQPLSFSALLAPCRLRGFACQRVLGLDWALTYNIVRPNRGSRYVDLMSACPPTRTALCFFEHPFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPP2</accession>
    <name>A0ABQ5SPP2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016870</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028928">
        <property type="entry name" value="CC2D2AN-C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056290">
        <property type="entry name" value="CEPT76/DRC7_peptidase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052434">
        <property type="entry name" value="Tectonic-like_complex_comp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20837">
        <property type="entry name" value="CENTROSOMAL PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20837:SF0">
        <property type="entry name" value="COILED-COIL AND C2 DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15625">
        <property type="entry name" value="CC2D2AN-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24656">
        <property type="entry name" value="CEPT76_peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2028"/>
            <end position="2048"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="1382"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="887"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1402"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1492"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="458"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="887"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1402"/>
            <end position="1412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2051" mass="225246" checksum="E7905F7DE6C44750" modified="2025-10-08" version="1">MAAPVEDYQGMYPQAAYPYVTQPQGVQPRPAQLFPTVGAQPQPVQYQTSPELQQYQQPRYNQPGQSPQPQQQAQTPPLVGSTAGGYQSLHPQAAGPYDPQAQGSQPQTVGFLPTVGAQPTAFQSRSGPELQPLPNNQAIQAPLLQPQAQAPPVIRGIASDANPIQPNNTGGVPPADVPPRRGVFSFFRRGQSNQAQQQQQQQPDVEPPTDSADPELGGQPASELDRQQGSVAGSQAVTGQGGDQAPGPDGTQHGAASASVPDRDVLQIAMVSYEHSYAHREDSPAVAASVLLSFDVGRFSGAPLALPERLTAEREGRFAREELGLFRERQCVLSEHSLNKMEQRITKGAPLDKALLAQMDSGDAAAAEQQVAIMERRRQQQQQQQQLTVGNLPTVTEEPEGQQGEAGGQEQRSEVLKLPPPSSSQPAQQPQPQPQPGQMTDTPPQPAGAGGEASRDVQPQPQQQLPEMQPDGQRVVPRFDPATLQPHTTIMDPMYAFQWLDENGLMVYVPDPLRRPGRERPARRYDQDKEMMRTYVRRPVKAASLRGETRLYRLDIELGRLEFLVHPAMAQEDLLAAWLLSLFRDFKRREAVGLVMFYANKLAALEDSMLGAREKLFALQATEAQSDEVLDAYAMLAKIEREATEMRQLKEEEEALLNRTVRSMEQLYLQIQGLRRQQGYALTNLELVVHSKPPQELAWQQGVLTRERLDVLLIAAEMEEIAGLPKFPEPLPVPDLADFPLPNVNSSPKARGLGTLPLGLGMGVGGFPGPGLMDPLAAAMPTTAQKPFVFKVRRCETMCAAHAGLLSDITTRLEFLKNRPSADPEEPHLKELEEQAALVGRAPRRVSMTSEEQLELHLKAAEMVKKGEIFVSRMPQLLPILTINQRDPQQPQVQQQMTTPPQPGQFQQAPGGSPDAPSRAPMAFPLPGQLAPAPPRPGLKYGGKGRYYVKLFVNGFCVDSSEVTSLGENFAGDFKDTFTIQVSRFPESISVMLFERHALRDSYIGQVYIAIPGLAGSPHVDAQARPYQFTALTPFRPRLPTQGPGPVPLMPDTHFHDSSYNFNTIFPAGVLYVRCGWVSDRTAPGTRLADTVQPNTVITYDNPLAERPAAGEQAASAAMWNDIEAGKSLMPPRASVPIDRMLRRAAERIGGKANRAQIMRWLNEHVIDPNDPRNAPLLELLKTHEANASALGDLFRLDIFPDVAMKEDRAAARRMNVLQRRWQAGIYRAPDRSLLKGVRSVAIPAPLSTAETDKLENQYLDQLALLEQASEGTQLALARVMMARHEKAMQLQIGAMLTNSIKEREERIRAFALRVHAAAARVAGGLLMARRFRTEDVVNDAPLPQFKLELAVITQLLAPRRPLKRTRKLVKSLMTSVPRETQLMVTVQRAANLPARMVESGGHGAVSGGAAGRGRNLRRPGRFGGGAGGFDEGEGTVDEGPSAALSAADREAQQRNCFVEIRFRSLVQRTEAVPGEFPLFNEQIDLDVFRDDGSDQQQKGAEEGSGLEPSPSALQANNDLITFNVFDELIVEPSETAITRRRRAGDDDLSDPVRLPERERRFLGCIRVPLSAVYQMQVLEGTFKLEAPPVVLGYVQTSDRPPTLTVHMTMRPRLAAPAGEVDERVVSSEQAQVNRHAQKWLAALLARPECRSRILKVMAVDADGAAILLCRYIAATPLPPPLAQLAVAGGNVAAAGGVETLMLKVARFVSHVPYMEDVGIQKRRNDIWTTNAEFLHLTGGDFEEHAHLLAGYFLQLGLQCFVVMGTAICGARSHYVLTTGQPATSPSQPQQPPPSLDLNPALLRLWNPVTGTVMAAKDPTGELREVGQLYDGANIWANTQPTGRPWEMRWNLGAVKDWHPFFGVALPPREIASLQTTPLYEELDSRFYEELESRVEKRVEEALSKARSTFVTKPDNKVSRLLKSLLREMREVHEKIAAASLKASAELAETGGQGPTDAPAYLGERHKLIQGLQASHNERIRREARAEAAHGHILAMSFSDRYLDAVSEAVTNTGIHRVADERVKFSTAVYVEPLGAAFVCCIWIYVVAVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RL20</accession>
    <name>A0ABQ5RL20_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000001">
        <property type="protein sequence ID" value="GLI58381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="157" mass="16416" checksum="7F8F7017064B97A8" modified="2025-10-08" version="1" fragment="single">ITNATNTTNATNAITAITAIATAATTDTYATTATAATTATKETDAKIADTASIDAEPPRSPPQPSSPPPLRKQQRFTIRIETGKTLYEIESRWLHVYVDTFATTITTAAIATTITTTIATTIATAGHYRRQPATNAVTAITATIAITATKPPSTPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLR5</accession>
    <name>A0ABQ5RLR5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="20130" checksum="2C3AE839E5B74BBF" modified="2025-10-08" version="1" fragment="single">MTVERAPYIAQQPVESEGSGASDHDIAQQLMHLEDMLFLSQYEEAAQASAELVSQFSERGKADDALLQRALMIAFQAHFFIGRHSGVDAMVDKLGLTRVGVPTFMAWVLLLLESGMASTARRRVEQYMSTVMALIPEQGREASSHSAIDDAAVTTGPQPGVDGCERQGQAPTTAAKVAESTADKSCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMH1</accession>
    <name>A0ABQ5RMH1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclease associated modular domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000524</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003611">
        <property type="entry name" value="NUMOD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34199">
        <property type="entry name" value="NUMOD3 MOTIF FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34199:SF2">
        <property type="entry name" value="NUMOD3 MOTIF FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07460">
        <property type="entry name" value="NUMOD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclease associated modular" evidence="3">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="582"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="714"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="513"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="287"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="618"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="635"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="660"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="720"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="780"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="812" mass="85269" checksum="143B8FEB4464F518" modified="2025-10-08" version="1" fragment="single">MEYAIGMVQHRFVPRIAKGCKRLHLKSLAIRHHTLTTCRSILPTFSLRLCIAKAAHDSSPAELAAEVPVPSQNAKTEELDEKERLRRQRISLANSGKVAWNKGRKHSPETIAKIRERTKQAMLRDEVVEKVKEGRARQLSQPVNQGIKERIRSTLLAYHAAKRLGDTSASEGSDDGSLAPEVVGAAPNSEMVAAKSKRRQSSKSLQPSTPVNTPEEKAVVHEVDTHTGDPTDDGKQATQPAKSKFRAAAVAARSANADHDAQTPLAPPPVLPRPKRRPAATSDSDIPAVAPLATAAAGEGAGEVKAPERRPGPPHGVKRSPEHRAAIAEAIRRKWQDPTYRASAIAGIRRAAGSDTGARAGGAARRHQAGNRAKLPERGLGAGINAASVSGETGTEAGSRRGKARKSNVSEEELPLTVRSSRVGRAGTRGGGMHKSQDGAVHSPSSTNSTNGNRGGVSVGNDEDSSDSSDTATSASSGGLPAGGGSSHSVSPAARLAHVQRLVRQVLVAERLVANTENLLRQFTQRRHALRNDPVIRSQADEQLRSVTATLQQAKGKLELLRAKVPPDARYNEKGDVWFAPHVPASSSGPQASRPPRAATGAGSGSGKAAGSSWGGIAHEEETGDNRGTEDGVAGRRSSNSSTSSGSGGSWPGGFTWQGSGNGNGNGKGKGLVVPATVVDVEGSGVDTNVGADPSGKVGVTEYWGSNATNTVALSGNGAHSDRWQSDYQHHVHSCSRERSGSGKGNGNNATSGLKGPANSDGLNGDTVPLDPYNERYYTDLDEEGEDEDEGEDDEDGFWWNSGWGHSPPSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RML5</accession>
    <name>A0ABQ5RML5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000419</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="952"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="584"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="832"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1112"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="707"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="724"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="769"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="839"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1120"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1151"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1184"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1197"/>
            <end position="1213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1224" mass="124070" checksum="728688C51CE1A558" modified="2025-10-08" version="1">MDKPLDNVQLPARIDDSELGYAPRYRQSAESSDDLDEPLAYKMQYNSVYNVTDGISSRSIHSSGWKVPHVAGKASSDLNSALGPAAFFTALEPGTLQPGGSDNSDDEDSLNRIAGYTRSASLSARTAVETAAAIAARTAALSERLSFRLRTESEQQVLHTQISTELTTSVDAKRNNRDAALTVAPLVATDVAVEAEAFPSSLLNQEGVAGEGTDTAFFQGTIQPMQAWQPANQASPGPPQAPPGPDPPQSLPICEVPGNQAEFEGSGSYLAAGDGTQVQQLAMPSEEVEPYFRNPTLPSAQASAWADTIPGAPSVLNEGWPPASAGSVPSAPPTWRKSEVRSFEDVSLDLATGSAADAEAWQAHLPRPSSNGSVMGTVGLPLDSAEVVATIEHPVFDDPDVSGRDHTVPCPESKPLTALAATADTPATSNADQLLDGYRIAAAAGLISPELASLLGLVPPLAETATAAEEGQNLVAEEEDTGDSPVRAAALDGLVSEGKSAAVGAALAAAFFPQPAPLAFTGLAQQVEGDPGAAVPAGDTAVVGGHTGGRNFSTAALVQHDHAGDPFDAADTDDEAAEFIVRPPQTAHRASASGPSSAEVRPGYPKEQEFVVPNTTAVAQGLHYALQPGPQAGVEPQSLAQAARPPEAAATAAAAGIGVAMVGGAAVAAAAAANQQAVSVVGGTLRSLHGSANSAISVSGHTGGVTAPAGQAGPQALQQQQPQEPLPPSLYVRPPAPGGPCSPQGLTLPTSMRSSASPITAPGPQQPLQQPPPGPQLRPAGPPPPHLAAQAPGFGPQANFPAGRGPAGATLPSAVAANSVVAPGATPLLGPYGGAPWPQGGPGPGPYGPGPIPPRPGLPLRPLLHPGAPLGAPRPGMPPLPGQGMGTVSLGFSQAPPGGGMGPGLRPPFNGETMAPPFGGQVGVLRPPLASGAAGPRPPHVAWFPPPKPSPYPGLLYTEGRDTLGRPVVVLNTAMLPAKSKKNDVLQYLLQELQPVVKGDYVIVVLSLAMGVKASSVSSTWALGAYSSLAKPYRKNVKHIVLVQPSAWARALLTLAQPFVSKKAAHKVKKVDNLVQISDATGGEVKLESLGGRFIREIQYGLGAPPLSGLVPTPGAGVPQRPPVPGPMVPPPRGPMPPVHPAQPYQGPPGSYMPPRPMPPGSGIATPVYGPGGPRPATGGRIQGPPPPRPQFPPGTSGPGGMGVGPQPGGGNDFGAAVSPPVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMT8</accession>
    <name>A0ABQ5RMT8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="4">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_000639</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22962">
        <property type="entry name" value="DD_AtENO3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF56">
        <property type="entry name" value="CYTOSOLIC ENOLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="9">
        <location>
            <begin position="88"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="8">
        <location>
            <begin position="225"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI58870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="526" mass="56815" checksum="6C0B8888FDB72939" modified="2025-10-08" version="1" fragment="single">QMIILQTCGVHQVCSKSQRPVFCLPIAKGRRAAVARSSRVTMSVQEYIEKHGLEKKVEEVLNLCVKEKPEEPLSFMAKALQQLSPPEIVKVVGRQIIDSRGNPTVEADVHTRKGLFRAAVPSGASTGVHEAVELRDGDKSKYLGKGVAKAVENINAIIAPALKGLDPTKQVEIDNKMKELDGTDNKGKLGANAILAVSIAVCKAGAAEKSVPLYKHIADLAGNGKLILPVPSFNIINGGSHAGNALAMQEFMILPVGASSFAEAMRMGCEVYHALKGLIKAKYGQDACNVGDEGGFAPNIGSNEEGLNLVNEAIEKAGFTGKVKIGMDVASSEFNTVDGMYDLDFKNQPNDGSQKKTKEQMLELYKEFCHKYPIVTIEDPFEQDDWEPCGKLTAEGICQVVGDDILVTNPARVKKAIEAKAVNALLLKVNQIGTVTESIEAVRISKDAGWGVMTSHRSGETEDSFIADLAVGLATGQIKTGAPCRSERNAKYNQLLRIEEELGADAVYAGEQWRFIAWEAEQPKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN50</accession>
    <name>A0ABQ5RN50_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000803</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="427"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="430"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="659" mass="68908" checksum="2E679C3B88251F85" modified="2025-10-08" version="1">MSFMTSTISHSCNEALVEAADPAMSPDLPTSLFDVYRWLRSELDVASRRALRECSKAAQATHDREEASIHAKCSLSRSEKVSVTEISNSISGILRRGCRPDRVSLLFDQQDTDAQSRGTAAFACFPAGDVGSVTQLVMQRFNLTVAAATAAAAAAPRLASLQLIECADLGSLPLLVASTRYTLCQLSLGGCGSWPGGVGDALSTCPNLEHLTVLLPAAITSATPTGTFYSSGLGPIQSDDSTAAVDSIPFVPVCSALTKLTQLRSLTMELKLPSYGDEHYEGTRAIAAAKAAASLARGISALTRLSRLEVGDGGALPHPEWRMELLHSLAGLPRLGEMTWRRLECDPEVSFALSSSSSLTSLSVEKFCTVAPVTAVALAAAATAAAEAEMTAANATAPGSSSVPLPRALASAAVAAANAAAASAMLGTGRPTGRGGSDDGGGSGHGGLFSSITTQKRQQQHQQQLKLLLLPLPPRLRVLRARYSGLGGVSLAALAALRLPPALTSLELRNPKLEIGDGDVNQPSAARLLPAAALALGMGLSKLATHCSDLQVLRIINETRQRYPVAGPFGWEEANGGDDGAGGGGHTPWMVNLGKLHHLKRLTLSGLRLGRVDAETLARHLTSLEELYLVPLGSVSSAAVKVFRSMKLRRLSLPFDRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNJ9</accession>
    <name>A0ABQ5RNJ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 9">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_000972</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 9"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF47">
        <property type="entry name" value="MALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0520">NAD</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="10">
        <location>
            <begin position="31"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="174"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI59145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="340" mass="35342" checksum="7471D534B2A32500" modified="2025-10-08" version="1">MASQSSAALLKWAQAASRGFAAAAASGPGRKVAVLGAAGGIGQPLSMLMKMNAQVSQLSLYDIAGTPGVAADVSHINTKAQAKGYDKDGLAEALRGCDLVIIPAGVPRKPGMTRDDLFKINAGIVRDLVEAVGKHCPGALLNVISNPVNSTVPIAVEQLKRMGVYDKRKIMGVTTLDVVRAKTFYAEKNGVDVASVDVPVVGGHAGVTILPLFSQATPKVTMPQDVLEALTKRTQDGGTEVVQAKAGKGSATLSMAYAAALFADSCLRGLNGTPAVECTYVESTITDAPYFASKVKLSTEGVDKVFDLGTLSDYEKEGLKAMMPELLASIEKGVQFIKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP41</accession>
    <name>A0ABQ5RP41_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051083">
        <property type="entry name" value="GrpII_Intron_Splice-Mob/Def"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025960">
        <property type="entry name" value="RVT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34047:SF10">
        <property type="entry name" value="GROUP II INTRON-ASSOCIATED OPEN READING FRAME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34047">
        <property type="entry name" value="NUCLEAR INTRON MATURASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13655">
        <property type="entry name" value="RVT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="136" mass="15703" checksum="51593A4D8C7C217B" modified="2025-10-08" version="1">MIRYEWNEIPWRKLEIKTFKLQKRIYRAMQQDNIKLVHKLQRLMLASTSARILATRKVSQDNRGRKTAGIDGKIALTQRERMNLALSINLKGRSKPLRRIWIAKSGTNEKRPLGIPTIEDRAKQAPPPPPPPPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPG8</accession>
    <name>A0ABQ5RPG8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052658">
        <property type="entry name" value="TPR-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15544">
        <property type="entry name" value="OSMOSIS RESPONSIVE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15544:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="139"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="214" mass="23809" checksum="5813371B96266A24" modified="2025-10-08" version="1">MCVYLDIFNVNTYGKRLVPVLRHIDTFSPATQAHSMAHSAKRQNLGRILQARLQAFADESSEEESDPIALAADLKERGQEHATHGRFSEARSLLGQAVRLVPGRADLHKLHAQVLMEMGYTWEAVRAATRAVELQPEWAEAHVTLARAQRNLGEPVLAEASFKRALEHEPEDADIVSGELREVRALATRQRTMGEGVRAIVQSESHEFNKDTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQD3</accession>
    <name>A0ABQ5RQD3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="33822" checksum="05835E226D0596A6" modified="2025-10-08" version="1">MHIAMLKRFLSVRIGGQALPQLLRDADGRTLLTGDALGCQYCSIPVMDQTLSSTSAPTLPPFLRELQDARHPPRSSIIRAIDKQLHGGKSSTGDDIDDKAPYDGVSRLLLESNGVTRANLDTLRLLQRYNIGMSLLRGHDILAKVLNVDGKRVLLDTGFTGLSEVPRSDVTVADVFAADGQLPARLFTTDVWPGDLLRLRVECVYTPYGDMQLSAVKDDIEQRRRVIWGELQRRMEQQKLVAGRVLNECPGGFAVGVAGFVAALPAALAATATTKLVGVRQEFQIIAMDSARQRITLRDPRLQHFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQG9</accession>
    <name>A0ABQ5RQG9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001461</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10289">
        <property type="entry name" value="GST_C_AaRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02799">
        <property type="entry name" value="tRNA_bind_EMAP-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053836">
        <property type="entry name" value="Arc1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF33">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21972">
        <property type="entry name" value="Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="6"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="TRNA-binding" evidence="6">
        <location>
            <begin position="270"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="403"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="45357" checksum="C5BC6EB2D98A0AD5" modified="2025-10-08" version="1">MSYESGPVAAARFVARCVGSSEQPAASTSGHVQLATGNGSVLRGLATVCTHLVRQSAGKHDLLGSTPELQAQVTEWLTWATIELVPLMDDRLLKLNQHLTSCTYLVGGSATVADLVIYGLVHPAVVSFPAAQLLHFCNLLRWCDLLQNNADAAGFFQPRLCLSKPRYMAPAAPAPSAPKVPPAAAAPAAAAPANVAPAAEAKGATAAPTKEKVTKSAPSKPAAAATEPSLGPALDAKGGKKEGGKEAKGAKKDGAATTLPAVKAGEEDATIDMLDCRVGRIVKVDKHPNADALYLEEIDVGEEKPRQVISGLRNFVPLEQMQDRVVVVVCNLKPAKMRDVMSYGMVLCASDDSHGKVEPVLVPDGVPIGERLTVEGFTGNPVEEINPKKKVLERLFPDMKTNEAGTPVYKEKPFMTSKGPLSSSVPNGWVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQI0</accession>
    <name>A0ABQ5RQI0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminopeptidase P N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001760</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF1">
        <property type="entry name" value="XAA-PRO DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="6">
        <location>
            <begin position="81"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="553" mass="58372" checksum="34376BF473DF2FFC" modified="2025-10-08" version="1">MLLQRQALGLGARRALILSHPGQLCRPWSRGPTSPVVPVAAAQSSRAAVISTVAPPAPTPAAAPASAAAAEFSQRFQILSVPEQFRQNRQKLCREFLGGGLLYLEAGEAPPRNGSDVMHRHRSDSYFVYITGVELPGYAALLDPENGHFTLLAPRLPEEAAYWVGAVPTLEQLQEKYGADRVCYADELKGLLAARDSPTLHALPATAEKLGAKVPAGCRVLTDLLPATLDRCRAVKSDAEVGCLLAASVASGAAHMDMWRACRPGLREYQLEAVFSLSSRCRGAPSLGYPSIVGSGPNAAIMHYEAADGVVEPGHLVLVDAGAEWRCYTADISRTFPALGRFEGAARDLYGTVLAAQYAALATLATPPPSGTGNGASLQDADRAARVVLLEGLREMGLVRSDAGGGSWLEAALEVKLDRVFMPHGIGHHLGLDVHDVSETGPVPKGPVQPGHVLTVEPGAYLIPPLLARAMADPRVAPFVNFDVAGALIRSGMGGVRIEDNVLVLSEALPGVTAAGLQGRLYNLTVAAGVVKEMAGVEEVMRQEWRPEAAIMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRB5</accession>
    <name>A0ABQ5RRB5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001846</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S41A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06782">
        <property type="entry name" value="cpPDZ_CPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07560">
        <property type="entry name" value="Peptidase_S41_CPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.44">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004447">
        <property type="entry name" value="Peptidase_S41A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32060:SF22">
        <property type="entry name" value="CARBOXYL-TERMINAL-PROCESSING PEPTIDASE 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32060">
        <property type="entry name" value="TAIL-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00245">
        <property type="entry name" value="TSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="166"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="568" mass="60357" checksum="C5785D6DCF5A964D" modified="2025-10-08" version="1">MRSLVTQRITHKNAPASLRCQERRVAPARVWLPQAVPKDISSVKNTSDVWDFKDFAKRIAAAALGTTTALSLILSPALAASELVRLPASTDPSIFAAQRTLVEAWTIVGQAFVDPSFNGHDWESELRDHMMTAYSSSSSSEAFGEISRMLEDLGDPYTRRVPPEEYAAFRVSSDGELQGVGMLIANEPINGHLLVLAPIKGGPADRAGILPGDEVTSINGMSMEGWNGEKAARLLRGRGGTEVRVRLARRTGGIPGVPARPEPLPAVEYREVSLRRERVQLSPLFFAALPPPALPPGTGALMPVGMDGRVRQPDGPIGYLRLTSFSSNAAAEMREAIQELEAAGASSYILDLRNNPGGLVRSSIDIARLWLDGSPVVFNVSGREPDEIQSQLVDAPGSALTHRPLAVLVNSRSASAAEILSGALHDNHRAVILGDTNTYGKGKIQSVFELQDGSALFVTVARYQTPNGTEIDRIGIVPDHSCVLPGAPASDDDEGSGSSSFMAGLPMVPGSEVALLESLMGDSCVVAARDVLRDEVAIVANAARVASDIVQPVAMQSTPQTTILPPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRK1</accession>
    <name>A0ABQ5RRK1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_002260</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="846"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="258"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1167"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1214"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1410"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1505"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="270"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1214"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1226"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1260"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1297"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1320"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1416"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1522"/>
            <end position="1533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1555" mass="158275" checksum="1709C04E3F1E32D7" modified="2025-10-08" version="1">MTDADGGHPPLWAQRDMTISDTPAMAAAAAAAGGARPRSGSRPRRRSHSNAAQRRWASINTLSTSTWQVAFGGGGPTAPPAYGHLMASEARPHLQSRTFPAGGNPTKSPNYRVAVQGMAEASQLQPTKGGVDGATAIATTVSSATVITAAADLDTSAPTPYTSEYCLMDAAASGCEPVPSSSPTRGGMGGVQPPSSAVPDLNPCEMSALAIADAADAVAAAEKPRPIGRYGEPWVASAIAAAEASRPKALGSDVAMDKARPLPGESQPGMGAGGDGAHGRSSRGGGESRGGTGNGYGGNGASSPPILAPRGQVLVTYNSYCSDASRDSRAPSFESNLNAPFTSNTSMVDRTKAHDASVALLAISPSRLGGGFGAGIGGGSTVTSAALSSRIVTFAASLRTAPKTYSAASGTWGVTARAKSDRRRLGRYIGGSGATFMASSLSRASFSQPGPRHSNMFSSAVDRSAFWTVRYTNGDSSVSDEALYGIEPCADDPDSTFGGNSRQRRFVSSCYNSGGGGGGGTAATARDKVSRLRAVSEHARTLIRSVGAYRRIASQRSCAIGLGMAAGSTAAAAAETKQATKAAGVAGVVPYINEEIQACEEDAMPTAAVGLTDAEVVVTGGLSPGGAIHDSANAAEQQRKPHGQGSFRLERASGTVLGAQHRKRSSLMRRGTEDYETTGAGAVEAMVSTNTAEANVTDGAAGAGAGAGAGAGAGVTGDVELSSECWHEVVAVGIVDPASGRRGVVVMQRDVTAKVVAERHVAQVSETEHRLLEQIFPRHVLQYIMEESGVCSAYAEEASAAELDADLERDHVRRSATPPLDAASIARWRPHVRDCNRLAVWHPQVTVLFADIQGFTPMCKVLPPDVVMSFLNTLFTRFDSMLDMHHVYKVETIGDCYMVAGGLIREDEDGMAAVQGGGHVDPDQAANVFAFAKAMLVAASAVSLPTSGAPVRVRVGIHSGPVVSGVVGTRMPRFCLFGDTVNTASRMESSGVAGCIHVSEECFQLLRQRDPDAAMKGWEATAGVEVKGKGLMRTHLWTPEHLKAQIAITVPPAKAAVNTTGTTTLPTGITTFSATTTTTVATVATTANTVRTTTNSAAAFAVCPDPPEQLQKIPHKPVLGTAVAANTDTSRAVSLPSLLPSAATPAPAAPLLGHAWTLLVSRNKGRQRERDQALGHRVTSVGGSSGCGGLAESSMSSALTAAIDTIAQHGAKNSNNTTSLVQQPTGAASVASGSGASGVTTPGDSAGRGDGHVSDGRSQAGCDGRSASAGGSADVAGVGGDGHRRGDWNGSGGEPSNLDKDPRAGGEPDSSKEAGPQINGMFTGSCHTTSTDLRWSSRHAMDLLDTIMCTTNNSVALGSTSTRRALVGASTGGSGRGVHHMLLRDDSQAPAPAASAAAAAAAAPAAAAGAPVRGASDARQHRTEHRLDSVRNGKSSVQGAGTSVGVINSGLAGSEAGSVGGQNANLRGLNALEMANMSTASSVMLVELLGMGEFLAPVVRRGRVGHGGESDKGGTGSVDEGVGIGHGGGGGVNGRSIFYRKRRNSLSGSLPDKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS32</accession>
    <name>A0ABQ5RS32_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="712"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="735" mass="74653" checksum="F1EF6E764D78E4AE" modified="2025-10-08" version="1">MASYGEVGAVISGLESEYVRSRKFLADLCRSLEKHGILKPSDRIEDSSVARKQNDLPDASNQSKDVIDPFANYDLGKESSETSVCSESSTSSPQLGGELHPEPPCSKCSSQSPAYGLNISTGNHGAGDAPLAAATSSPARHRHISGRIYKKPSVVFSSCPSTEPLTQDYTCTERFTAAHSCNQDHASGLQHRHSVMGAGNSTVAALYHEGPPSLALVIPPQIPRSGARHRRSSLDFVPAPPAHLRKWLDATASPCQQQQATTSSLSLSLGAAMASLPPPSIVSGNVVQERPYQIRRRSSIEVLGCSAATSPANLSNAVSATTPVAMTFGSNSAVNHPNSPSAGCGSGCGGGVSILSRPCLAMATMKRSSLSVCGNHPLQQQPQQQQQQQCEHSPPHPKQQPPQQSSAMESCPGVVAPAASCSSPQVPAPPKMGIERSASAGGGQSGFGLGLPAGNGSNHLPPLHVAISASLPTAISSPGAKGGVTELGGSGIGGRSSSYSSSGSFAPAAGVMRHSSFSGRPPSGCSSAAPASALRPPQTVLEFSAGGSFSSRDTRVRSGRFSRCSMSSVAITSAAADSAGTSANAAAVATALLNHVHVVQDKDRRSISSGEVSERLIPRLGSLTFTGTNLVDGSGADAGPGSDGGSPLSARYSRRGSGLSCGGGDAEAHATICNGGGGAAAAAPATAMTASSFAAALTTAAAAAATRLQHMTPVLISPRRSEATRARPSPSGQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTS3</accession>
    <name>A0ABQ5RTS3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="8">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003270</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF35">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="9">
        <location>
            <begin position="30"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="492" mass="53639" checksum="61369F5F61F63638" modified="2025-10-08" version="1">MTGAQDDHINGQADTPAMAAVFPEAHKPLAAADPELFALIEDEKVRQWKGIELIASENFTSLPVMEALGSCLTNKYSEGQPGARYYGGNENIDKIELLCKRRALEAFGVSPDEWGVNVQPYSGSPANFAVYTALLQPHDRIMGLDLPSGGHLTHGYYTQGKKISATSIFFESLPYKLNPQTGLVDMDKLEEKATEYRPKIIICGASAYPRDWDYQRFREIADKVGALLMVDMAHISGLVAAGTLASPFEYADIVTTTTHKSLRGPRAGMIFFRRGPKPVARLLKGEPEGTAYDFEDRINFAVFPSLQGGPHNHQIGALAVALKYVATPEFKQYSKQVVANCRAMANALLKRGYKLVTDGTDNHLILWDLRPEGVTGSKMEKVCDLCHITLNKNAVVGDLSAMNPGGVRIGTPAMTSRGLTETDFEAVAGFLHEVLEVCKEVQSNTGKAIKDFVKGLDGNARIADIRARVEAWASSFQMPGFTVPDVVAPVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTX0</accession>
    <name>A0ABQ5RTX0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyltransferase small domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002865</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="26745" checksum="3A3CE0BAFBAB494C" modified="2025-10-08" version="1" fragment="single">SMVELQIAEISKTHWNGDVYEPSDDTFLLVDVLQQHAKAWINKWPFCCVELGSGSGYVITSLALLLRQLAGPSFGHPAVHRRTCQLLAVDHSLAAVEATRNTLQAHKVTEVDVLATSLLGPLVDRLRGSVDIMIFNPPYVPTPDEEVERSGIASAWAGGYRGRRVIDRVLPLLPELLSPRGELFMVTVTENDPTGIIEEMLGFGFLGRIAATRQADEETLQILHLWRQPAGLDAGAAATGQERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUV8</accession>
    <name>A0ABQ5RUV8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="138" mass="13740" checksum="EEA5E7D7BF894100" modified="2025-10-08" version="1">MAAYGLRPLKCAPGVLRGGSPPTSPLATPPPRNISGPLPLTLMSCGGGRAPGTAPKPTVVPCHKSGSIYGVQGALWCPLTWTARGTAARCTRWAHPSPAVASQAQSRVCGDGASGGGGGGGGSNVELYRATAATRRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV26</accession>
    <name>A0ABQ5RV26_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF38">
        <property type="entry name" value="ARSENICAL-RESISTANCE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="76"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="190" mass="21710" checksum="5174F886D8943B32" modified="2025-10-08" version="1" fragment="single">CSCFSFHLVFIAQHAPALLYFARGVAGFFLRSFRRRFFVPDFLRSISVTEMAIAIRPTNQYLEPLQLANVLRHPTQKERILVLDVRDEDFAGGHIRGAVNAPSEEWCNEAHISDIIDKHLDGKDMVVVHCMFSQQRGPRCALRLAKQLSTRPGPAKPIVYVLRGGFNGFVSQYRHDHDLVEGIEDAYWKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV45</accession>
    <name>A0ABQ5RV45_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_003432</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RETICULATA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021825">
        <property type="entry name" value="RETICULATA-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31038">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31038:SF10">
        <property type="entry name" value="OS04G0524400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11891">
        <property type="entry name" value="RETICULATA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="85"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI61148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="410" mass="43324" checksum="AFE7BE4824549A3E" modified="2025-10-08" version="1">MLQTDVLSRAVGRKPPVVLRRLIVCARHVPVMCSLGSGLKAQHVNEQTSSAAASSVLSPLVISLNDAVLLAPFVHPIGDRNLRAEATAGGDDGSSLGGGNEGGGGGGGGGDNSGGGKGDGEDDDRVLALSEVEAFAAEKKISLPADMLEVAKKYGLRLSVLNAFVAAQGFLLSGLICRTIPYFRDRILADPLFLFKVGAEVVIDSGCATVAEVRKRGKEFWSEFEFYLSDLLVGLVLDVVLVSLMAPAAVLGGVSRAAMANSPLKKWLATIPSAVFEASVPGVKKYSLTQRVACLGVKFLEYSLAGICCGLIGQGLANSLMMLRRQLHGTKEDDVPVPPLFKTALVWGLFMGVSSNTRYQIVFGLERLVDMTIARSVPQVAYGTTVAIRFINNVIGGENFIDMARWAGVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV57</accession>
    <name>A0ABQ5RV57_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UGP3-like C-terminal hexapeptide repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057388">
        <property type="entry name" value="Hexapep_UGP3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="UDP- GLUCOSE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952:SF14">
        <property type="entry name" value="UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25441">
        <property type="entry name" value="Hexapep_UGP3_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UGP3-like C-terminal hexapeptide repeats" evidence="2">
        <location>
            <begin position="767"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="UGP3-like C-terminal hexapeptide repeats" evidence="2">
        <location>
            <begin position="878"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1050" mass="111919" checksum="9E10C6A495E6733E" modified="2025-10-08" version="1">MTSRGPIGASISGASSSQRAAAPMPLQSLPAPCAIRIPRRGSSAFHITGRNVARSAASGQVPQPHQPRNPSPPAKVPLTAAAATAAQAAAAAAGLNHTVHQRHLELQQQSPSAPILSGTVTSYEDEYQLRLDSELPLELQIVRLQALLERLRTAGTSEDKVRHLWAVDSVAAFACDPRNEAVCTALTQMTSAQQLLLLSLPAMLQEHVLMEPPSAPARSPEALLPHLTSLAARLARVDQFYDSVGGLLGYQLKSLQLIVAGMQDKSARNKENEQHTAGAADTAASDAAANPVAPPPTQISFRVPPGMDLAGEEGSGVGVAAAAQGLASLPFMAEIYPVGGAGDRLGLMDEVTGESLPAAMLPYAGRTLMEVLIRDLQAREYLYFRLTGRQVTTPVAIMTSDAKGNHDRISRLLADINWAGRSPENFRLFRQPMVPVVGVEDGRWLLSRPMGPMMKPGGHGAIWKLMWDEGVFDWLIKDHGRRAALVRQISNPMAGMDTTLLALAGAGFARRSGGAAAFGFMSCERAVGAAEGMNVLQERRRWVPEDGSSASGGGRWVYEYGVTNVEYTEFERLGLNDEAVSANSKTSVFPANTNVLYVGLYGAKKIVAEAVARADGEQLLPGLIFNLNKKVSYTDPLDGSTRQVTAGRMESTMQNLADYLTDRFDRPMGPQELAATQLSTFFVSNLRRKVTSSAKKRRQPGSARIAQTPDGSFYDLQRNAWQILQRCGLEEVPEPGSPEQYLEKGPGFIFLFHPALGPLWDVISQKILGGSMTHGSELVLEVAEARLQQLHLDGSLLVYAENVMGHMEAVGLPAAAATKEHGNTLYGAIGAAAAAAGAGATASATLSVSSIHNGTLEVPLPRAVAHGGALQQQQAAAAAAVMGGADTAMSPAPGGAGRLRFGPRCGRVQLENVRIRNKGINWQDPDNVYWKHQVSRRESCRIVLLGQSEFEAHDVTLSGSQTFVVPDGFRLTVTAAAEGTGFRTQLTPLRPASPAPGFEPSWEWIYSMDTSGAVRLSYVGAALRSPAAGMVATAMAAAELRGQYVLDFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVD6</accession>
    <name>A0ABQ5RVD6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-ascorbate peroxidase</fullName>
            <ecNumber evidence="3">1.11.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_004010</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.11" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044831">
        <property type="entry name" value="Ccp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002207">
        <property type="entry name" value="Peroxidase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356:SF8">
        <property type="entry name" value="L-ASCORBATE PEROXIDASE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00459">
        <property type="entry name" value="ASPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="9">
        <location>
            <begin position="114"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="333" mass="35463" checksum="2987A05C6FCE3473" modified="2025-10-08" version="1">MVEILQRTPCSATRISPTAGTTSRNCPLVRADAARQRLVTRRNDLDSVVNLSLTRRELLGASMPLLALIGGAAPVGLVAGTPPARAASGLVTPSSAAQAAMNKALSRAIPKAKTAVALRLVFHDAGTYSALLGDGGLNASIQYELDRPENFGLKRGWRLVEQVRSDLRGTAAEGLVTDADLVALAGAFAVRLCGGPDIPLRIGRPVATSPDPPGRMPGENFSAEQLKANFSEKGFSVQEMVALSGAHTLGSKGFGDPTRFDNEYYLALLRRPWNNPNDSMASMIGLRSDHVLPDDPECLPYIQRYAEDQNAFFADFSSAYIKMTTLGVPGWAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVD9</accession>
    <name>A0ABQ5RVD9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="111" mass="11518" checksum="6158FCEFC2BF3E9B" modified="2025-10-08" version="1" fragment="single">TRGSAEAAADKTSNIGEKIKSGIHGVAEHVHDAAAAVVDRARAAVSSSHDTAQDVHDKAAGAVWDIAEKSAAKASLAADDLRTATRDAYSRGEEAMDEACRRMASVSKTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVL0</accession>
    <name>A0ABQ5RVL0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003383</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045439465" evidence="3">
        <location>
            <begin position="17"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="576"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="522"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="802"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="536"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="853"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="920" mass="93235" checksum="962A297700C5FE44" modified="2025-10-08" version="1">MHLSVVLACAFVLVFGAAPLYGASDSSTLLPRPTPVSGIGNATAALAAAGLLGVPLRVDYRDRDDAFDLKLLSVATGMCSLSGGCNPCTYRDSNPCQVADRYGADPSLCHLPSFLGGNVTSDTLCGIGATVDVTFTGGGSKPIATLTAFRKRGGSLLLTVTARCGWVLLPFVSTTLNQTGNSTPARVIMEGNASYLGSSSYYSRQALGANGEVERYTCFTTVLKLRGLSSSCQALNLDLNLKLRMQRLNIVDRNATISNPECQVGGAEVEATALLKMRVPQAYSNSGSIKAAAMSEEEAAAAKQKIFTNGVVATTSYFASVDPRDPQATSWLQSLASQFPIVSAVPSSETLSFLIGSVRKDSRSSQVPASDQAPLLPEALSLETCSRQSITAVTEAVVSALAPKGITEKDVSDVVCLQSPYTEVAATEVPPCAGYTSVLYNVTLNITLGPERVSGTTSSLLKGLNAVSQSSSSSGTVTPASPLQLADALVGAQLAAPWVCLSTEGQMRMISRFQLDVQLPKGPGVVEKTTTDSGYTGPAATTTTNPGPAANPDTAVARSGNNSSPATPDGGSKNTFGTVAVVAVAVAVALVVLVALVAFFAIRSVRRSKAAAQAAASAAAAATATAATLAAARGRYTYGTGALSPPPAGKLAPSTSSLSHMKKLYGEPSGLYAETSYASTYDGLGKAASPKADGAAAGIGGGAAAAVATEEGVMPWAENPSSLSPATSAAALEPFAAAVEAAAMATAVPAPPVAPTGERYTIFSGIGGMAPSECSEEEGDDAQLASNRVPMSPISLAVPPSAAGRVGPVTPPAAAIADGSQSFNARSPTSPPSGQPTPLRRNRTLKFPAAISAGSNKSNSPLAGSKNEHNVGMKLGRPEASGTEIGGSVAVGSTVWSNPMYNAPLLSCSSGDATPPASEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVU7</accession>
    <name>A0ABQ5RVU7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003693</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="167" mass="18185" checksum="BF6DDEF560BD265B" modified="2025-10-08" version="1">MNHADPFAGSHLESVLFQNVNMHTLPLMSAQAQFPDTCDQDKLGSKGHTLWGNLDHGRPHAIMLPGAWAMGDGLVLDTDAYEDQSGRAHPSLPWNVESISSQVARLACSFRSSVGGYIADLTTAWTGDPNRDDGAMDNAEHTTFIEGEIAWMPRASTCFVTADKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW20</accession>
    <name>A0ABQ5RW20_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Membrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_003609</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the GOSR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF1">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="102"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR028865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI61414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="227" mass="26218" checksum="51D22157A51817FE" modified="2025-10-08" version="1">MSDLTSLHSQAKRLILLLREGLERLEALEGSTTRPPGSADATSYLGRDLKAKLGDLTRISGEMDSIWRMQVIRENSSKRDVWKRKVEQVSEELDSIRHALERHTRRESRREAEQREREELLDRGESGRKAKQEMDEEALVAGSVQRSKRYLEEMYDAGANILVNMAGNRERLKSAQKKALDVLNSVGLGESMLRLIERRQRLDMYTAYGGMIFIVVVITFCVWLFWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW83</accession>
    <name>A0ABQ5RW83_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004409</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="129" mass="13295" checksum="909649191241DA2E" modified="2025-10-08" version="1" fragment="single">GMSHTLISESGKVVVRVDMTLTELVLPPREQNGTFNNSSDSSSGGEGAGVSGNRNGTFKEAPVIRSRSILLMTQLPRAPPPKSPSPPNVSKSSIGSSAASLRTSNSMANTLDTAYGLTSGSKTTNPFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX27</accession>
    <name>A0ABQ5RX27_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="505" mass="52482" checksum="BA40E873A810B192" modified="2025-10-08" version="1">MESQQTAAQQSIQSTMQGLVCGAAFVNHCIHPQFQQLADVQWKIELSAKLAAILEKLRERFSKSQNVCLCQTWVGADGFDAAPSNSLEVTLWADPRLAIVLDPLFEDLRAKSCQGPVFFRNSMPGKVWRSGAVQIVQNLRILPPSLHPRSQLSEAALERLAEALYIPIYDLSRPARGQVCVLEVLLSTRATEAMLVADVISFIGNLLTALHLSLANPVQQPVRRSVLSGRRPRAPDSDGEASNDEGASPSGRTSDPQRQQQVAGGPQSRQEAQAAQEAGPGPRDVRQQCLGEDGGRGGTATRSALSAEAGTGVGSCHHSASDVCRGGASMSTCGSRSGEAHANGMAPVVATDGAAGIAMAGAAAAEAGHVEARCVRGGPEAVPSCSEQPTLPASPSRERLVRPGATGHMAASSVLTAGGPGVHGLGCNGVSAYPGSESLQVPRKLRKVLDGPMQRTKSVVSVTALSQGAAESEDDADGNRSGQGQAPCKGTGALAHCLKEAPAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXF2</accession>
    <name>A0ABQ5RXF2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004868</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045080136" description="SRCR domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="4">
        <location>
            <begin position="35"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="389" mass="41922" checksum="AF1A5B09BE0FC46F" modified="2025-10-08" version="1">MVNLEMGAFAVAVLLLISTSGGYAQLKNSRNKNGVRLQGGTGPMGRIELSSLNSWFNNGSGPAVKPAWNSVCDRDFDDELAQIMCVMLGYSYGRKAYDPKFNYRKDPNPDGRIGKIFCTPLLPASRGLRGVTTETTTTLSETTIGSRGSRRLINNRDPRVARSLFEPLRGSLNSPPKAPYMCTFRAGSCDSRGPLAGVQCSHTPLPPAPPPPPAPSPPPTPPTPAFPPPSSYIKLVGGRPVDKPEDNVEPNLACDGDVDCVSFGRLELKVPSTSASNTTIWAPVCAIEDPVLAMSVSYIACMQLFDWPDPRSDGIIVMVPELNPAFKVPTGPVSSASHFNPTNYNGWINITGAGPEPFMPEKVQEFQYTISTTPCRSLLAVQCQVFNTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXR2</accession>
    <name>A0ABQ5RXR2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14825">
        <property type="entry name" value="TRAPPC2_sedlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14802" checksum="5E426FA75A82C403" modified="2025-10-08" version="1" fragment="single">IVGQEDHPIYEVDLTGPKEQQTQYLHQFVLHSSLDAVDEQMWMSKEPHLKVVDRFNGLNVTAFVTAGNTRFLLLHDGRSDESIRSFFTEVYELYLRVLLNPFHAPTTRIMSREFDRKVKLLAKRLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXT5</accession>
    <name>A0ABQ5RXT5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004374</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="953"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1272"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1281"/>
            <end position="1297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2032" mass="212649" checksum="107A73F4157A4DD3" modified="2025-10-08" version="1">MSKDADETLALTGNPPRMPQKQHEFLSWLLVTAQWQVQAPALLLDWSSDGGDGQPSDAAEATSGAGDATGCRPLPPLPGTLCTDSLATVSRLILFLLLREPCIAATDESWAQHYLHQVMLLAPSLRPVDLVPTAKLVCHLYESHPTLLARSAPEWASDGLGGDSGAVSNGPLHALVASACSSIECLSHSQSTAMYSSITALAAKPQVREQLYSWTAMGLPAKSSHCLVAPDIVLLTRATPCELSRVASSSTSTRSRTSSGGNFRSCAKTAVAVASAVRAHMHGDDDVVDAAGSLRALLREPSREEAAGGQCGWNVAARDLAPELVGGFREDARSTRSPIEVPTGCRSGGAVAASSRPAASGAAASATGVVAIITNEAEAALDPAAATPLAKTGAMAEEAAAIAVAATQLPRGIAAMAAALHSTVHTGAAAVGETTASSELSAAGANSTIASRLALQAWTPGAPPGALTFLRVFARSSPQRRDDELVLLAPPRRPLQKKTATSTAAAPVIPAPSPAYTEPPNATPVVMRNLPSSADAEGNNSAAINGALQALARLLRSFAEARVPLPIQQLQEVQTAWRRHLHILALNGGGAANSGGCSTTPPSVADVRVVAELLLAVAAAAPRQASVRRTAVLELATTLGASGAAALRSLPPMQLTACVHAVAFAASGGGGDGSDSSDDVGRIGRIGRGEMARKTTALVRAMEPLLEEFQRRMALSGSQQLHHLLPPQCVAVVCRALSLLSYGPIWPARLRRYEAVVAAATNSQLSTATLSPYRSSSSSPSAGAHLRRAIAAYLPPLTAAAAPPPSVVRYGVWVLRHLDLQVGRNWLERLCTCAPRYLRRMHPRATADWLAACASRCHAPPRPAMRRAWLHLYYTWRLLNWHELYEVVWSAVRLGLRPPRFLLAAACQSVAAAAAAAGAGAATARPAETNALHGDTAGGALITAAAAVGSDCSSKPHAEPVASERVAADGGARRRASDPGGGSGGQRTAGCKALQIASISVRLLWAVQQDERYTPTPQHTAALCGLMAAVWGDLAAGERTAALWALASLPPLSSSERAVLESGLGDVVVRHLLASVTGSDAGGVSNGHCGEAELLTAVDAAAPGGPEGSSRVEGGAARSLSASDMARLVWALSRLRQPLPRHLSEHLCAQLAVAVTTAEMPAWSTAPMTMEMETIMMTEASYTTSTAVSMKDSHRAGLRPKEVVQALYGAARLLLGTCHAAAGATAVASLLRPLLCATLATDPALLDARSLADALGTLSRFRRVLAHHRPSDHPFRRQASLDGSSVTLMSDQNSDVNQRARSSAASRASVSSDAGCWGNDPWVVAALERARELLEQSAARGLGDRPVPAWCALEVLQAASFLGAPVGPQLAAAASRLLMMHLPGMAPAQLADVLGCCAAHPALPAELAWEAFQQLVRVETHLAAARGDDLEPGFETAANASTWPPLQPDELARAVMGAPQAMASLISGHSGQALQVHGIPESMAHHKCDLQVSNASLVPGQPEYWEEREVWLPPPLQQRRLLAAAVAKLACRPGDPRVASMTPDQLSMLVQGVVAAGAAPGVAWLGWVCGLMQPRLKEASQHGLLRMCKALEVAEMWPGQAWAQACLEQASNLVKARRMLRATERQIHSALLRLRALGAATTTARKCNGSATRPPAADAATTATASLRADALVAAETLPVACGPVSSELGHKIAPAKAAVDSIAASCSVRDGNGTESHVGALVGQPRSTARLGDLLGAEARQAQRLVYGLHDAFAAALQLVLKRGGLDENTLGGPHNPQQQTASWGYEEEKRLPQRPTSDAFSQLLLVYRLVEARTDRVMGLSLKVAALQFAYALQVSNSRQHQHREMALQQTGPRGLQGPARRMELLRNGFDQRDDTGAEDCVSLVRAQATESTLIDRAFCGGAVKRGQVRERGGVLSRRTLLEHTSAPVLESAESLLPLATYLREQQMAPPLWLGRCSEGKVSRDQLQVHAVAGEQEVGHRRPTVWGRVGGLRGSGRGAARGDDSGTLEGGDILACIVHAKYCGLLHLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYF0</accession>
    <name>A0ABQ5RYF0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_005121</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF79">
        <property type="entry name" value="PROTEIN SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="633"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="729"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="282"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="796" mass="82790" checksum="272A0F2070E71C49" modified="2025-10-08" version="1">MSVVSAHTQPVASDFSLVKTFPPLCLGSHRWSTSWKHQLITTHILSRSIGKHNGRFVSSQFRGQHGSRVPIGVLARDGGAPTGTLSTVVDPDIIVLGLSAGTHSSVAPVGSTIGTPVSQPSAHLAQVGEPGAELINPSSLVTAETLAGDANACRAVQQGEEENVGYINQSAEGISEQGFAGSTTNLSGHSAIAWSASSAAKLSTAASMYVDAQSASSLITGSGSGADAMPNSQPIVTHAVVDGSIGSLQNAHSERKQTFQFTVLWDRIAAALRSLQWEKLWALTLLSVSYIHQATTGFALPAMLPMISNELHLTDIQGALLTTGYSYLYAVALVPIGLLADRMSRPKLLAAGLALWSVLTCTASTCRSFGELMLARVGFAAAQGAQNPVCFSLIPELFPVNKSTALSLYNCAIYVGRALSFAAVLLARHLHDNDQAHLAARGGLRMETRLDHLDRADLHSLSIMHTAGDMAAATPDKEFNFPLWEYGNELPDSAWRQVLRWIAVPGFLIGLAMMATLPESRQVAPAAPPPPPLAAVAAATSSTQSTVAPTAGVALPAPPLAEKFSATMPAAADAAAVEEPAADTAGNGIMALLRSPGFMSVTLAAALNDVGSYALIAWQSTFYERVYGLESSAYAPVLAALLPIGGILGGVGGGYLADRLSRCGRRVWVTAGATCVAAPCLAVSCLAPTPELSYAALLVGFALSEMWRAPSAVMARGIAPPSMGSTASAVYLCVRNLLGGLGPLLVAGLIGVCGLQQAMMVVPTMYLASGFIFAEAEKLYDVEMEAKAKAGMVLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYL2</accession>
    <name>A0ABQ5RYL2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004859</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026050">
        <property type="entry name" value="C1GALT1/C1GALT1_chp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033">
        <property type="entry name" value="BETA1,3-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033:SF50">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045160797" evidence="8">
        <location>
            <begin position="31"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="241"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="558"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="568"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="789" mass="84153" checksum="313B117423BB5C91" modified="2025-10-08" version="1">MQARGLLVRPLAIALLILLAFSCNRTAAKGREFYKKSAATGPTFKAEDFIVVINTEQARLQLALASRPLRAGLRTFIAVDNLTLAEELNQTGYKYNETYAYFPNRNITGPERSKPGDTRWQAAPFMAHHHYGPTYKWILLGDDDTLWFMLGVKRLLYGYDHNMPYAISDHFGDHNARGFFLPSPLAAVCSPCHWPDLLRAHHREDLLPYSSAVLTRQEVATWDPREVDAWGVGKMKIRISRGSGANTDVAGPERQQRGSSSTPQRRAAAETGSGDIRNQTTSTSTSMQHLQYPVWGRRGQEPINMTAVLDIAYYSTAPGFEPAAMALSAATAAAAASAASGPTDLLPPPIPPPGCPCRPAGGCLHRARVCRGRGRGQELCSRTLVFKIGFRGNRSWCRHSWAHGGAGVVLSLGLMRATAGGLVEACARATQVHSCDMNLAMCLMNPAMGLNGSMAGGGGHEFNNSGGGGGEVAAEGLAGGPSTGAGTGSSGGGYMFTHPGSAALWGANWADPRFILFDNPVYQRVLHDPLGLLDGRVVCGRDARVAATAAAAALALRAGPEVEEEGGGEGRRGKGEKGGSVGTAPGGERRRLLLAVADEDAGGNGLSDAGGTGWSDVQYGGEMVGGDGVPGRYAAKMEADVGNGDPGDDDETVAIRQHEQHEDRVFDFAFAGGASEGRSRNPSDDTQHVVRFGRGLRKVQERVAVAVEVASRRGAAGGGQGVRLKRRDGDLEDINDDKAICGWLVRHAVSLHMHGRSYASYNDAARAMTVVVRRHMSAVRRLRDLQQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYR9</accession>
    <name>A0ABQ5RYR9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005503</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="132" mass="14889" checksum="5A90A4B3BADB34DA" modified="2025-10-08" version="1">MAKPNDNEPKGDGLQSEELRLVTLRPPWTGHGTSGRRKPMAENVALVQRVLAYSKMEDKAIGDHLIFQVAPEVYQHQGLKSVYDRLRCQANPCHHQLGGEYWALAFKAFEHLHAVYHRLEEAQMLASGSGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ37</accession>
    <name>A0ABQ5RZ37_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004924</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="617"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="783"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="892"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1026"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="748"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="617"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="789"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="803"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="901"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="944"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1038"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1051"/>
            <end position="1064"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1131" mass="112352" checksum="A8CEB1123A759E75" modified="2025-10-08" version="1">MFRRRNIKEQEQKDVLEKEVAFWKTEMEKVVNEKNIWVERYMKASEDNEMLRAELFLVQQEAQSTRAHLEVLRVRQESRMAHSPAVGVGSSTGTPQSGRAGLAAAGPAVGGSFGPSATSANTSPSKYGGGGSGIGVVSPCDRSMVQHLEGQVADLKAELSRAHRQVAAAEAAAHSADMRVALLEERLVEAVQELEAARRQTEFMRATAEAHQREANALRSEVLSQRNINDETANETTKILSELSSAQQRCAKLASELSIKEQMVGLLKLQLGDFAATAAVPGVNSSTAGLASQLAAAAAPPSALPSRSVSLSSLGWGLAPLLPSGRTSSGGLGGGGDGVVPSAEAEPVAEVQRERPAALAGGGGGPNVVEWPPKVLNSPEDGGKSPSLIVLDGDDGREGSSSAGGSSAIGGCDGSVWTAAAEAAALGGFPGSIAIPASELTVCCGRSANGTLSPSGPGGSFKANGQVSAAIAAGAAADVTLSAAKIVDAANGGSEATAEVPRGTKLAFPKLTSVAALSGVAPSSATAANPSAAVAVVETAGPTFQEAAMQVDPSPSHQPPAMLRKILSKGFAAATAAATAGSPQVHPLRPHLHYHQQRPANVPQLQLGSALRGLGSGAAADANTATPTADNSSTHGVRVSTSPAAVIGKRVAAENAKEDEIEAPALKRTFATGMASTMTGTAEPLLSCAPMVAASIEEASEETTEVIVAASAVAVVAEATAAADIAVMVIVDGGERKPVPSDGGELGQGLAETAAEVLKEKVENEEKEANNAAEMQRFEKSCAGGSMSHGKENDDRASEPSKTTSDTASAPSIPSASGALVGTEVTAAGGAAKTAAGPAVLAPRAFRFPGNRAAAAAAPVSSTPPKAVGSATAAISTVSSVSGADGIPRTPFGFAARRSSGGSGASDGGGPAATAAAVVTTPPPPSAAEPVLATSTPGGGNGAAGTASASRGGVSNTLSLLKRFNSGGLGSEPSRDRSPAPLAVVAAEGPTAGSSLSAVAAAVRRISGVGATDVAATLTSGQDTCLPGNTLNSAMEPPAQPNQPTPPKRLTTGTGVSTLLNKFNSVSGSGTPPTPPPAPGSVSAAIGTATASGSGITTVVVPEYGIGGPATRVPASAKIAAMRSAYEKNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ67</accession>
    <name>A0ABQ5RZ67_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_005779</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 12" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="14">
        <location>
            <begin position="106"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="377"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI62928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="481" mass="53982" checksum="59378386F776C03D" modified="2025-10-08" version="1">MADGGEPPPKRQCVQNLQIDSSGSLLPQGFASGLVGAVQQESGRDTHGSTSLTQQVQRTAENLISEPAGPGPLSSRDRERIATGAYSQREEALIKREQDGDIAFRYVLNNNDPQNLIYLVGLKNIFSKQLPNMPKEYIVRLVFDRRHRSVALLKRNGAVIGGITYRAFHAQTFGEIAFCAVTSHEQVKGYGTRLMNQTKEFARTIDRLTHFLTYADNNAVGYFEKQGFTRDISLERERWQGFIKDYDGGTLMECVMHPQICYIGLPDMIHAQRIALDDHIRRLSHSHVVRPGLTHFIDTATAGGFGRSSRYSVIPIGDIPGVREAGWTPSLASVQQQQGVGGGGSKYRLVLDRGRPPADPAPENLQVFLRSLMEYIQGLEESWPFKEQVAVQDAPDYYDVIKDPIALDVMEERLASGCFYVTLEIFNADLRRIFENCRFYNAPDTIYYKLANKLESLVNQYLTTHLLFDEQEEEAANPAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZF3</accession>
    <name>A0ABQ5RZF3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005298</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="240"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="631"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="748"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="836"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="915"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="676"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="189"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="636"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="658"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="748"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="856"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="915"/>
            <end position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1002" mass="102435" checksum="2DAA781963A193A4" modified="2025-10-08" version="1">MTWFFQKEDRSTLRAELQKARDELHQNKDEFVKLLRDRESDAEAVRRMLAAHSSELTRVKATFEEQKSVSQSHLEDKAKLQLELIDHLKLLRLRDERLGLLRRDLADSEFEVDQLRVALRCTTLYTLQLEADKQAAEALIQHVCAELDRVGAVVAASLDAARGQRDIPAALSGLPPSSEAMNPAAAPLSCAASSSSHSSHRTSSCSSNSSSSTGGRNHKVACCCPNKPAEAEAEAEATIATMRDSKEVDRQQTERQRSEKSIGEDDSDTVAAAAATPAAAPLAAATTSLEMSVAAGGLLSLLDVLGRLPEQLWSVRQQLHGLIVHPKVAAAAAAKTPTSSTLNETATAGGCIGVDTAAATGSGHATETFNGCGNGGGGAPPCELATPRVPPPTVVEALLQLCGLAEGKPRRTGNETLTEYDAGIGSLTDWGHRRHAATSCAEPAVLARLMMGPRGRRDGGGGVKAVGVAAATASPPASEPLTPHQRPDVLAGLSEALMGWGPADGGIRNAILVQGRRMAGAASPSPALSLLSSPTRCSSVYMPTSPAPSPTGAAKAPSCLMAVAPPCGETKKSGHLGGTGLQASTQPQGCGGAGTHNAAVTATGAAAAGSTAAGAGAAAATAVASAVLDSVPPRNVPSSHPQTESYQRQEGQEGLTESHPQLRQQSAGNEQGHLYVRDLDSELGSLRLTNEALQRQVAELQRQNDLLQLRQLGGGSEGCGDGGFSGGGVLGSGFGGPLVSELFRAPPHQQLHSPHPLPSHAFGSSPGGRKAAAAAAADRQQHAISAAAAATTSPYNSPLSAPYYYGSLPPGQQSPTAGFAAAVTRAPAVVILPRGSVAVTDPGPTPPRPGRPEAMSHQTLRSTNGTCKTLRTRNSFASGAAHNQTATAAGPYAGGAPTSARNQQGVLGQEVVLSRRDGNHHPDGDGAAMTLNAGKQHDSSSGGSVPPPAPPASAAATLVAAPLPLSGALAAQVPAPPGVDPALWELELLVACSKEAAAGLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0X7</accession>
    <name>A0ABQ5S0X7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosylation/Crystallin J1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005801</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF17">
        <property type="entry name" value="SELENOPROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="41758" checksum="60C423283C1A8E8F" modified="2025-10-08" version="1" fragment="single">MALRSLSVGGPKVISPAAQTRSVPKVHRQVKLHAAAPLAAPAPTMTDSSVKERAKAAILGGLLADAASMPLHWIYDVPKIQELLAGTSRETTPEFFPEPSCPFYKHPYGSLSPYGHELIPLLQSMAENGGELDENKYAEALYSYFTATEPEAYRNKSIRNVVAAWEEGKRGREVGDREDFQANCFAKASLLLARYAGKPELREKVEAAVRVQQNNDKAVAYGLAGALLLEKVVLGHTVEAALSWAVSGGGLPDDVRVLVERALATRTQPLRELTYEPVSYMAEMVSKHMALRDSLPPFTLAVWCNGPACGNPAAFTNVAMAAAQYGNKYVDGVRANLVAGGDNCSRSLLIGALLAAESGLSALPADWKSKTTKFAEYESLVDKVLMAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S149</accession>
    <name>A0ABQ5S149_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Retroviral polymerase SH3-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006308</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0229">DNA integration</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Retroviral polymerase SH3-like" evidence="10">
        <location>
            <begin position="54"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="206" mass="20861" checksum="64514D2C3917D30B" modified="2025-10-08" version="1" fragment="single">MLAESAVPKEYWHEALMVANFVRNRSPVVGKPKTPYELLTGVVPDVSLLRVFGCVAYAHVPKGNRDKLDSRAQKGTFLGYEANSKAYRILLGDGRIQITHDVVFLEDQFAFSNHVRPELEVFSIQDGPSVPVAGAAAGNAGQAGGAGVVDAGVPAATHAAGAPAGNAGVAGVGATGGAPVIPAAGAPAGNAGVAGMDDGEVPPLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S152</accession>
    <name>A0ABQ5S152_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006656</name>
        <name evidence="8" type="ORF">VaNZ11_011065</name>
        <name evidence="9" type="ORF">VaNZ11_016292</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="7 10" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000074">
        <property type="protein sequence ID" value="GLI66975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="470"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="697"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="704"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="742"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI66975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI71184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1352" mass="149256" checksum="BAFA4D0C55ECDD0C" modified="2025-10-08" version="1">MGKDRLDEPLDLHNYSTWKLRFQLLCAELGYSRALLQAPIAEADVIISDKVKSVMAKNVKNHHLQTIVRAANAKAAWDALAATFASTCNTRKVALRQELSDFKMANGEELPIYISRARQLQSDLLDVGHAVADTELSTIVIKGLPSKYHVVTTALTMREGELDFTQVVARLMAYVIPDEDNNESSSAFITHRSGGKFNPTSRHQTSKQRSKSNAICHYCGKPGHFIGVCKKRLADQQQQNNGRGGGNLQRREPTKMAYAVGSESRSDAWHLDSASEWHITYDASELKDLRAVRPEEKFTLVGYDGSRHQPTHVGQYTMWSNTVKGLQIELKNVYVVPKATVKLISAGIFDERGATILLGNDKALVQAKGRTILRADKVGRLYAIRHHAAGRSVGKGRSCAAVGSEGSKAALWHRRLGHLGYKSLERMCREHMVTGMDLDRASLEAARSRICEPCIYAKQTRGPFPSTGHKAAKPLGLIHLDVCGPMPETSIGGSRYVTTLLDDCTGFSTVAFTDTKDAVRKKVKIMIEALENMTGRRVKEARTDRGTEFLNTVLGAYFGEKGIIHGTTVGYSPEQNGAAERLNRTLLEKTRAMLAESGLPQKLWAEAMATANLLRNVSPVVGASVTPHEAFMGVKPDISHLRVFGSTAYAHVPKEKRNKLQPVSKKGVLVGYEQGRQYRILINNLISTHSTVKFDESTVGDRYEDSDEEDVEESGGVPLPGGGGDTPVNTDMDQGAAEVNHGASTSSGQTAAPQRHAGIPAAQFPRMQNTAQSRGGNGGDAMTGEGPVQQQNHNNDGADVNMQERRYPLRERKRLIEWRHEPNGRVTFGRINAAIEEEVPEPASYQEATDGPYAEEWRSAMDEEISAQLTNGTWELAKPPPGARILPCRWVYKVKRAEDGEIERYKARLVAKGYEQRAGIDYGELFAPTSRFASLRMLLAVAAAKAMPIHQLDVSTAFLNGELEEELWMQQPPGYESADPEQACRLLRSIYGLKQAPRCWYMKLAAELDKLGFKPSVADPALFIKKDEKEIVYLLVHVDDIIITSEDEAMIRRVKTEIGQAFKIRDLGEAKVFLGMEISRGAHGEVKLSQRRYIEELLQRHQLVNAKPRKTPLPPGTRALPAEDNNKELVDNTKYRALVGELNYLATSTRPDISQAMSVLARFMGNPSKVHMGLALGVLRYLAGTKELGLCYDGDGDLTMVGYSDSDWAGDPATRRSTTGYVFLLNGAAISWNSQLQRTAAASTVEAEYQATAAAVREALWLRKLTRDLGLRTQAIQIRTDSEGSMSLGNNPIMSVRSKHIDVQHHLVRERVLRKEVALSHCSTEDMVADVLTKALGEIKFKKCITAMGLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S166</accession>
    <name>A0ABQ5S166_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein SYS1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_006643</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019185">
        <property type="entry name" value="Integral_membrane_SYS1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09801">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI63645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="18074" checksum="6FA7ED2B6AF0E1A5" modified="2025-10-08" version="1">MASKTDWETKTIFKKLILYLLGYYIVYYVWSIILVGALHVDWHQLGKYPFRIQRSEFSPHRRDDALGAWLAMVLTYICCMGLTYGVVKATRKSWDYVVTSSVLHLLLCIIINQAFPVNWIWWLTILLASFILSVVSEVVNYYLRDMREIALDNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1J5</accession>
    <name>A0ABQ5S1J5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05255">
        <property type="entry name" value="SQD1_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="84"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="478" mass="52759" checksum="A5999EBEAC5BA746" modified="2025-10-08" version="1">MQCPAMQNPRVGRRSAKAAAQQGVACVHIGGRVFASRVSGQRKDARAASAASRAKSVKVHATATIEKPAGPTVTPSNGGAGTRVMIIGGDGYCGWATALHLSARGYEVCIVDNLCRRQFDLTLGLDTLTPIATIHDRVQKWAEVSGKYISLQIGDICDWEFLSQAFTFFQPDHVVHFGEQRSAPYSMIDRQKAVFTQTNNVVGTLNVLFAIKELKPDCHLVKLGTMGEYGTPNIDIEEGYLTVTHNGRTDTLPYPKQGNSFYHLSKIHDSTNMLFTCKAWKVAATDLNQGVVYGVRTEETMADPLLINRYDYDGIFGTALNRFVVQAAVGHPLTVYGKGGQTRGFLDIRDTVRCIQIAIDNPAARGEMRVYNQFTEQFSVNQLAEIVSREGRKLGLEVVVQNVPNPRVEAEEHYYNAKHTKLVELGLQPHLLADSMIDSLLEFAVQYRDRVRMELIKPAVDWRKTGVKVNTMGAAVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1U2</accession>
    <name>A0ABQ5S1U2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15465">
        <property type="entry name" value="bS6_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_bS6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS6M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="Ribosomal protein S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14225" checksum="486C784E76319CD1" modified="2025-10-08" version="1" fragment="single">ITYTSLIPKASHKLKAGMPLYELLCLARPGPRAELVTLIKSLGDAVYKNGGFVTQLKSFGEQHLAYDLRKPFEKYDSAHVWQMDFVSSVDGLRPLDHQLHVSDKVLRWTVIKRPLGSKPAHSALADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1V0</accession>
    <name>A0ABQ5S1V0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Clusterin-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006981</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLUAP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019366">
        <property type="entry name" value="Clusterin-associated_protein-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21547">
        <property type="entry name" value="CLUSTERIN ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21547:SF0">
        <property type="entry name" value="CLUSTERIN-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10234">
        <property type="entry name" value="Cluap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="303"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="303"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="316"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="375"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="50420" checksum="37217C5C55EDD380" modified="2025-10-08" version="1">MSFRELRSYSEVMKALGYPRLISMENFRTPNFELVADCLYWLLQRYYPGVEISDDISTEADRVKFLQSVAQVMLTKARMKLNIKRLYAADGNAVKELLKLATLLYKATSKAGDIDDDATEVVDVNASLKGFNPKEIKMSASEIIKAGAALYDALGQEPELREHRARAVAGHVDTDFVDRSIREAIAMVEDNIHSLENQMEDLERNEKTLDNKIEKKKQELERQEKRLSTLQSVRPAYMDEYERLQGELNELYQKYLDKFRNLEFLENELETYYDAEQEKMEAQERRLKKMQKRLKEEELRILRGEQEVDESNLHFDDDEDDDEDDLSGEDGEDEDMDDDTAYQAKGAARRGPVVGGGGQGGARQQGRQVMGNLDGDFEDEASIDEDDNTDGGDVSRAGSDTGGGGGYGGAAYGAGYGGYGQAGYMNGGMDEDDGGHFIQDDDDDDNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1Z8</accession>
    <name>A0ABQ5S1Z8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_006668</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07859">
        <property type="entry name" value="STKc_TDY_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="121"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="11"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="525"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="648"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="528"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="572"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI63639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="673" mass="73600" checksum="CC0D1E3FDEBBCBEB" modified="2025-10-08" version="1">MRLADRVKCWLRKLGPSDNAAEAPAITVEQRNTGEETAASSLAGAGMAVGATSDSSLEEPPAPPTSVQPPQELPPPPAAQPQPQPASQAPPQASANGTNGKSVLDTRDFFAEAVHAEANRYTIKEVIGKGSYGVVCSAVDNFTGEKVAIKKITNVFEHVSDATRIVREIKLLRLLKHPDIVDIKHIMLPPSPKDFRDIYVVFELLETDLHQVIKANDDLTPEHHQFFLYQMLRGLNYIHSAKVFHRDLKPKNILANSDCKLKICDFGLARPAFNDMPTTVFWTDYVATRWYRAPELCGSFFAKYSPAIDIWSVGCIFAEVLLGKPLFPGRNVVHQLELITDLLGTPSAEVIAKVRNEKARRFLLNMKPKPGIPFELHFPRADRVALRLLKRLLAFDPAERPTAEEALADPYFTGLHCVAREPVAQPLSKMAFEFDRRKLTLEDVRELIYREILEYHPKMLADYLAGKSCPSFHYPSALESFKRQFAHLEAGGSLPARGQLGSRIYPSSSLPKEWVAEFQAEAAKYGSSGTGSQGGAGPGGEGAATAPTSTAARQLYSHDADGLETIGGDPQQQKDKFEESDGRSGLRGHADGGNVGQQQQQVIFQDQQQHVMYQLQQIYQQQRCGGRVVSAAATGTAVSAASHYRAPAGPPAAQAGPGHGPGTFRSESYSTCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S277</accession>
    <name>A0ABQ5S277_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein acetyllysine N-acetyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.286</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_006835</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the sirtuin family. Class IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF12">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="8">
        <location>
            <begin position="28"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI63761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="286" mass="31774" checksum="D8217FDFABCF4E12" modified="2025-10-08" version="1" fragment="single">MSLGYADRLKNKRNLGGQLGAKEYHQSFDEIKEGVKSLAAWVADSKRVFVFTGAGISTACGIPDFRGPKGIWTLRKKKIPISADLTPFEYAKPSFTHMAIAALVAIGKVPYVCSQNVDSLHLWSGVPRRQLAELHGNCFAERCTKCRTEYTRDFQMETVDFKPSGRLCSQPGCDGPLVDNILDWDTPLPEDELEEAVRQAEEADLALVLGTSLQIQPANEIPTLTRDEGGKMVIVNLQKTPKDRKANLIIRSRVDLVMALLMQELGLQVPPYIRTEGLVVEHHLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3F5</accession>
    <name>A0ABQ5S3F5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Galaxin-like repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055284">
        <property type="entry name" value="Galaxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056601">
        <property type="entry name" value="Galaxin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34490">
        <property type="entry name" value="PROTEIN CBG12054-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24748">
        <property type="entry name" value="Galaxin_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galaxin-like repeats" evidence="1">
        <location>
            <begin position="166"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="387" mass="40806" checksum="DB0F2670E00A8AE6" modified="2025-10-08" version="1">MCRRALTPSPSSPAMWSSFTPAAAAGMYRRLSRLLLPAVIILTFTLFMQPATAATCGNTTYDATLNVCCSGVVQPKKGSSTQCCGKVAYDPTVSVCCSGAAWYNLDDYTECCGNEAYDPTVSVCCNGFMWKIGKGYTQCCGNEAYNPTWSVCCMGVVQPKKGSSTPCCGSKAYDPTVSVCCNGGVRSKIGSSTQCCGNVPYDPTLSVCCNGGVRSKIGSSTQCCGNVPYDPTLSVCCMGVVQPKKGSSTQCCGNVPYDPKLSVCCSGVVRSKIGSSTQCCGKVAYDPTWNVCCSGIVQSKRGSSTQCCGTVSYDSTQNVCCSGVVRSKIGSSTQCCGKVAYDPKRNASICHRTQAKHEGKRLPDSQINTIYEPTCAPVITLFECNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3U5</accession>
    <name>A0ABQ5S3U5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DEK-C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037647">
        <property type="entry name" value="HIRIP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15410">
        <property type="entry name" value="HIRA-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15410:SF2">
        <property type="entry name" value="HIRA-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEK-C" evidence="2">
        <location>
            <begin position="4"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="562" mass="60337" checksum="F73CB20F93591AB7" modified="2025-10-08" version="1">MADKISDDRLRREAKELLDGKAKTLLDNNQLTMRIMRKLLEEKMGLAEGSLEARKKAICAFVKEALALEPDTAAAAATTKAPSTKGAATAAAAVPGGSTRSHKENQKPRGASDSCDRDEKAAKGGGKAMQADPDGPSDGAAPGVGRAKGRGRDVEKVQRRSGKKRTALSDSGAGSSDSDSSRERDEEEAGKRQKKRAKKTARGSSGPVAGEGGGVAKSKALSSRAIEMMKDVCKRATIKIGPSLYIRNKTVSELENALSELLARHGLNAHSRDKEIAQVKRHLDKSRDLEGIDTSNIISVDSGRPRRAAASSINFRQMFKQQKVDSEDEDEEEEEEEENDQPARKKRPTAHKASKSKARRNDEDDEDDEEEEEEEQANSQSGSDREAEEDKGEEEGAHVSARGGKATAAANQEKGKHKASALQKRSKGRNEVDGASEHSDDGGDDQAELSEELVDVTRGLGKGRGGAKADVRADEKGGPHQDGSDVSESAEDGLEDSEVEEAPGAAKKTVTTNAKARRVIDEDDEVEEEEEEGMEGGPSATEEEDIEEDTDDSDFEADDDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S420</accession>
    <name>A0ABQ5S420_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutaminyl-tRNA synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="2">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_007164</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="9">
        <location>
            <begin position="53"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI64011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="450" mass="46896" checksum="3CB970B0B2D813EE" modified="2025-10-08" version="1" fragment="single">MQAGLHNGLRSGSLRGLAFKAARCKHTAVSAAKSSVIRDIHDKITSKECTAVEVTQRYLDAISKTDPKICSFITVDADQALSQARAIDAQIATQGTEGIKVLSGVPLAIKDNICTQGTRTTAGSQILRDYVPTFDATAVSRLRAAGAVFLGKTNMDEFGMGSSTENSSYKATRNPWDPDRVPGGSSGGSAAAVAARECAAALGSDTGGSIRQPAHFCGIVGLKPTYGRVSRYGLISYASSLDVIGPMAHTVEDAALLLNAIAGADAHDATSSGQPTVDFAAGLLPAASLQGGSRPLAGRRIGLVVETLGEGVAPEIDAAIRAAAEHLESLGAVVEEVSMLTFSLGLPAYYVLALSEASSNLSRYDGVRYGVRTAGAADLPSMFCGSRGEGLGGEVKRRILMGTYALSAGYYDAYYKRAQQVRTLAQREMNFALASFDALLTPTAPSPAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4H1</accession>
    <name>A0ABQ5S4H1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022227">
        <property type="entry name" value="DUF3754"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33645">
        <property type="entry name" value="AMINOPEPTIDASE (DUF3754)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33645:SF2">
        <property type="entry name" value="FAMILY PROTEIN, PUTATIVE (DUF3754)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12576">
        <property type="entry name" value="DUF3754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="598"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="745"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="816"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="946"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1280"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="745"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="980"/>
            <end position="1003"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1377" mass="145746" checksum="4ED562FC9F59B488" modified="2025-10-08" version="1">MECTRCRVWPHGHESRVFRRSSDTWRAWTLHLTHLPAKLHQGSRAERLCRCDQASPVQATPVSGLPPLPPVQHVGTCVQVPHQTFLDMGSTSDRFWTLVTQTLVPPPERRDMTVLLACLESVYRAEYQQLAGEAITAIAAANNAHVRPDGTVAVPQAEADSGLATAAAQLGEQDGDQQQPQALHRGNDDGEGPLPAVAAAAAALAKSYGIRTGTINALTDSAGSSLVEMDTGGVRGCTAGGEGDGSAAAIAADLDDKAMRQLLRLAQRAGYQPLTVRDVRLADSLNTDYLSQLFIKGSTGRLDGALVQDYVDSSLPAEARPVLLLQRGYGRELQAGRLLLQKLDYLQTLAVGQAGSWASGAAAVLAALLFGTFEEGGPRVASLFVDTITGTEATADRRGEDAAARGNVDSPEVTVTAAASTTAAAVEPGDREDANELNDEDGNLQAAGAATAIKEVWIDCDNDNDEANEESQVFLMENDEEGLANGDAPEAEAEAVKSATQAGGLVVGAAAALAAATAGAARVSLKAQRLGAEALPKGSRQVHNTWQQLEQQQQQRAQGRDPLNPEDGEQAEPSGKGPGELQSSFPWLKRERGVALHRRADGRPVVGTLSAQGPDPQNSAWEAGSSSSWAPEVLRGRVGRPRGAAVATSLLGGSLTAATTAAAARSAMKLQAKGELDVPGEATGSGSTTAAAVGACSPVAFASAAVPSSPLKENDDASLAPDTIPSGGSSVIADLGEGNVIVRSSNVGLGSSSSSDCNSSGRSGIRRLESEGNAEGEVDSSDRDCRLRVAAETSAERAAASGSVRGNVELGMAAEAKPSTHAAPDSGPETSADPRAAMEEPQPTAPPQSRRLETALSTVYSALSNWGLVPRTDVIPLTSFNSHFPVRERPGRQPLFVCKVSLGDALGGTDWLRSGLRGVLDGLLREVQLQEPTFREVMVLYRPAHSRMAPTREPPLQPKLGLWQRQVTRRRPQGSSQQLDDDRNLSDGKDEATGREAGSGRERRERHHHVKRGLESGWLRRLWRSWRGVNEEEPAIRRPPIQIRIYRDIPLPTWKVVLPEKLLQFRPLDLLRVDIFSLAGLAGLAAQARYDSMLVDAITFGSAAVLIVRIVLGYQRMSDRFRSVVNELLAEKALAGQEGAVEAMAMAAAQQQLRQAALAYVLLLHHCRPRGHDLRQKQQLPATLGASGRSMAATRDGLEAVDTPAMATTAQLQGLAEWALAHYSGVKVRFNARQALHELHRLGLVVRVTQHENSGGDTSDDDGGDVDIDKAIFNSIELHGTSEAGKGSPALEGAGVKDSGDWSGAKSTSAAAGHQVPADGCGCGDPAADSDATDSRRSADVWWAAVPLRDAVPVVEDHWAGLLWRRVDGILSRRDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5W0</accession>
    <name>A0ABQ5S5W0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein root UVB sensitive/RUS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008791</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006968">
        <property type="entry name" value="RUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054549">
        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770:SF29">
        <property type="entry name" value="PROTEIN ROOT UVB SENSITIVE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770">
        <property type="entry name" value="RUS1 FAMILY PROTEIN C16ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04884">
        <property type="entry name" value="UVB_sens_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein root UVB sensitive/RUS" evidence="3">
        <location>
            <begin position="157"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="460"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="554"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="755"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="824"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="460"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="560"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="591"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="609"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="762"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="839"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="914" mass="99018" checksum="53893EFC0EC08293" modified="2025-10-08" version="1">MTRKQFATRKASLALLHAEQLPNAIELLPDFGNDLRFASSRGIRVLVKSGVGGAFSVCRQHRYHAAFCSVSAASGSPSDTRACAGKAKAPGGSSSGQPAHGQQPASPALERLPVAIKKGDQLLPYVWDGQRITAMSEAALQDADVTTGRLRNWFQGLQRQLKEAFLPDARDVTPDYWEWLRWRLTQRFFSSTMQNFSFSALLMATGLGAKKAFAASAAINWLLKDGVSRIVRMSVSTSFGQTFDADLKRMRFITSLIFTACMAGEFVTPFWPQHFVALASISSVGRAVGLSAFVATQPAFQAALATGGNLADLTSKNQAQHMVMDMLALGVSAGLTWLCRGMPRGGLLLPAIMYPICAAGDLTCIWHELKAVQLRTLNRERAEMIIERWMRRGAVPNAAEISAAESLVLPSDVWRGLMPLRIGPLDRIAPAGPERLRELLKEYDGEEYVLHVKRRCEAPAMTSSSSPSSSSFSSSSPAAASASATSSGGGMTGGVDWRLPSWFPSGVLLGSDGGGGQPEVVVSLSDRAMPHDIAKALLHAAYLRKAVLDHFASRHSEVHQHHHKQLHHHSHHSQQRHPHAAPQSPEDLLHHHNHMNHHHNHYNHHGHKRDLTNRGDDGPDVICRRDVGTSSHHGRKNNEHNGRGQFTGVQQLLHHRHGQAHQQQGLGGGAGSDGVGTAAVWTGHAHDHMHWDFHHHVLYHHSHYHTYGGEGMELGGERRHPHSHHVPVSGGLGTNEGPLTAAAVAAAARTASLSLSRRHGNGASNDSRSSNSTGDVAFGKVDASGACCPGPCQTLSEAANGQHKQEHLEGTHCGPVGDCGSGRFGGGAATSVPRRSYPGHSTDNRSSTCNGGVAAVELPRWERLDEATWVAMIKSSRQEADRNLTRLLREAEQAGWKLNPFMLNTTERVQFVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S786</accession>
    <name>A0ABQ5S786_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospholipid:diacylglycerol acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008503</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="897"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="669"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="907"/>
            <end position="919"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1047" mass="108832" checksum="183C833C7A784E6E" modified="2025-10-08" version="1">MAKQRKKKDAATNGISAGTQGAVTRTTATDPPTINSRPAGAPTSPGAPDIRNADGACDVDTTPSDASARHPAGAPAAASPYRASPHEQNQHYEYAPTGVPRRRRGRGGPGPGAGPVPVPGAGPGGNCREVGGGAGGGDKAGSTTEARRVTTSDRTASGGTSKTLTVKGHNSGTSTVVASGHGGHGGPGGSGSGASFPRRPHRLWRVLQWPLVFGVTLLALAAVHQRLIEEAPLTELFQDGIVGPFARLNVTLPRPSLDALIASTGINVSALIGLTDALTDTLNDITGTIIAGRLSLVSLLPQLPAFNLRPPMGLGGGAGDTEAAGLRPGMVMARRGYRAKHPVVIVPGFVTSGLELWQGLPCGQRYFRQRMWGTLAMVQAFMTDPACWFQHMELDPRTGLDPTGIKIRAAVGLEAVDYFIQGYWVWGKLVEALADVGYDSNNLVSMSYDWRLAIPLLEERDGYFTRLRLAIEALAEVSSERVVITAHSFGENVVRSFLTWVEADHPGWVEDHVHAVINIAGTSLGVPKSISALLSGETRDTAQLGALAGFLTSNLVPRATRTRVWRTWGASYAMLPVGGPGVWGNASWAPDDTPEMRAEKRTYGAMVSLWPHDWETVLAAAAAAAPHQREALTRLVHNVSEAAEPPPPPSVKPAAANPVEVVADDLDDSSSGSSSSGSSSSSSSSSGGPMARITDTLMRLVSSGGSDIDPADYLNSEHVTRLDVSGFLALLREVGGSLVDQHVREWAAVDLAAAAAGTQGDSIRSRAAADADSDTPQAQQVLGGARRLRENVRRRIALFPDATRTPLPNAPSTSIVCLYGVGLPTERGYHYLRPPPPPQGSASPSVAAAGAHGIAAEVVAAILSATVDEVSSTAAPSSAPLAGDAAVAAAGGDISLGSSSSPPLPHNKNNFSANVESSPDSISDLSGGWTILKDISNPGSSLDVGVQLSDGDGTVPLLSLGLMCRHGWRAGGSLNPGGMRVVTREFKHRSVSLLQDARGGPATAAHIEILGNEAVMHDVIRVAAGRLDELDDVIHSDIDRIAVAVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7K5</accession>
    <name>A0ABQ5S7K5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049804">
        <property type="entry name" value="Choice_anch_L"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038133">
        <property type="entry name" value="choice_anch_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047322159" evidence="2">
        <location>
            <begin position="45"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="771"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="796"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="826"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="916" mass="95102" checksum="79453897D9A42125" modified="2025-10-08" version="1" fragment="single">MRRCRGAAAAEAASTATGISVTRLFGVLLPSLCLLLATADHAAASLEGGSHDVLTSDGSAAAAKIGIEAASVRHSGNRGVATATSAIRTRRLGHRRRLQQIGNTCGSYNACPAAATAAQFLAGPGVITRNAVFSQGSCNPDSSIYGAALQQQWAIVPNNATWAPGHAIQSTFPQGSVVLSTGGTSTINCNYNSRTSTTTVIEGSLESDADLASLVPGQFLYDTVALEFDVTFSSAGWFTLSYVFASDEYAEWVGSAFGDVLGVFVTPSSDGDDSNTTNVAQVPDAGVPVLSSTVNAVNNNFSFVSNQLGDVANKRPLEADGYTRKLLTAPYEVAANQSYHIKLAIADTGDQLVDSWMYIGGGSLTLVPKLVESYDIVAPTAADCDITKFVSLTAKPAVSGATPMLFIWTFIANSTCLPSIEKSGASLRVPLNEFVEGATYTVRLTAFWNGYWETSSPRTINIASGCGGGYQPSCVISSSNTTASAVVAPAPPPPQPKAALTTLPVAPNGATVVVPWGGTAIVPCQGIVVIETRGINVTALLPDPKPNSAIFTWGVYNGLDPEQTAPFVFPVTSFSLVSGNVSSAAPIIKANDLRQTYATKRYLVRVYVGTQMAWQDEQGSWGGGVGSLAPGQTWLQVNGCPIAPSRTPPTLTFGNVALESVTLRCSATVQLDGSAPRARGEQFNVPKRPGRFTTLIWIVTLTGQNPREWRQESGANETIYLFDPLRLLVSTGLPYDVHSTFTVEVQARTVAGDLLSDSKWQIKSILLPCNGPLPPPPPSPVPPSPKPPTPFPPGTTLPTDAASPRPLTASSPSPPPSPLLSTPNSLPSPPPSPPPPSPLPPSPPPPSPPPPSPPPPSPQPPSPPPPSPSPLPVASPSPPPPSPPPSPLPPSPRPPSPPPPSPSPPSPPPPSPPPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7P1</accession>
    <name>A0ABQ5S7P1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lon N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008896</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46732">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE LA (LON) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46732:SF8">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE LA (LON) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="2">
        <location>
            <begin position="339"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="542"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="655" mass="67391" checksum="58D632BFEF1C2226" modified="2025-10-08" version="1" fragment="single">ADRPAAGTGTAAPAGRAELDDGMLPSSSPYPSSSSEFEGHFQRLLLDRDGAVTSSSNSTGGGRRRRPSPGSAAALSRKGGGEGDVSGSGGQRPSSAAAVSDAEDVAAATTGDVAAEVANHLLRLQSPFLVLNGKLHTSSGALLAIYPKVPARDAVTMAGTAAQQQPADLRLDPASCDVMARLALGSSQLVHVVLWGSTLQAISPSPTGPEPFAPSPAASDNPFYWQPAHVTLPGLQVLVGDSDQGLRPDLDDPNLSDSFPAAAAVTTAAAVVIPPAAAEAAAVGGDVPGVAGKGFLEGSGGQGDGEVDPEDQNEVADGEQLKMSTSALLASLRGGLSYLPVFPLEGVILFPGQSIQLRIFEKRYRLLARACVQQGAAFGVCWRGRGTTAVVRSYQTAKDGAGDLMVLLEGGVRFSYDADDLTVLPASYGLNAAMRAEYVVDEPPGNEEDAAALRATAHTVIDSVAGALQEATGPYAQQTARTFANALHFASSNSDSSTSSTSSSSNSLLAAVAAAGEVSSPRPHQQHHQKILAHSQQPQSVSSGDGASDSTSCGSDGGGAALLEEDLAEAKRVLDAETASLLSLYLAPHIPVHLESLRREWFTSRSALWRLQQEAAWLRSSPRVAMAVASSVLRLPQDHPLRIMLGLSRVAVVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8D5</accession>
    <name>A0ABQ5S8D5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Solanesyl diphosphate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009930</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02749">
        <property type="entry name" value="prenyl_cyano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI66167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="44193" checksum="BB9E23CC491FE43E" modified="2025-10-08" version="1">MGLYNMNIPERMSTPETVSCSSRVVSTCPSRHCFLATFSRNSHSARPLVRTCVYTPTRIEGQTALKLEAQVVPPANLLNEILAPVKADMEQMNHNLKNVVGNRHPMLMAAAEQIFGAGGKKLRPVIVFLVAHSTAQREGLTELTEKHRRLAEITEMIHTASLVHDDVLDECNIRRGKETINSMYGTRVAVLAGDFLFAQSSWFLANLDNLEVIKLISQVIADFANGEISQAASLFDTDITLEQYLEKSFYKTASLIAASCRSAAVFSDSPVEVKEAMYNYGKHLGLAFQVVDDILDFTQSTEQLGKPQGQDLASGNLTAPVIFALRKSTELLDIVSSEFVEEGALERALELVRETGGIEEARLLARQQADMALAALECLPEGPARRSLRLMVDYVLERIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S918</accession>
    <name>A0ABQ5S918_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009598</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006356">
        <property type="entry name" value="HAD-SF_hydro_IIA_hyp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01459">
        <property type="entry name" value="HAD-SF-IIA-hyp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF90">
        <property type="entry name" value="OS08G0542600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="399" mass="42882" checksum="ED273D2C6FD4A05F" modified="2025-10-08" version="1">MQVNVHGLSLSLWPWTSRKQNYHPTLPLYTFCPAHRSKQETLDTRRRRAFSGTHQPLTTNGTPSADACDTMIQITGLSEVSGRYKALLLDQFGVLHDGRRPYPGAVEAVEAAADSGLRLIIISNSSRRSGGTLDKLAAMGFERRCFEGVVTSGELTHRYLTLRPDDWWSGLGSRCLHINWSRRGPASLEDTGLQVVADPRDADFFLAHGTEALSLPGGGVEERPLEELKALLRGAAEEAVRARTAPPPLVVANPDVVTVDGDDLVAMPGSLAAVYSQAGGPVVLMGKPSPLIYMACGEMLGLAPSEMLAVGDSLEHDVAGALAAGVDSLFIAGGIHARELLVEREGSGESVRGQQHSAQKRVDVEALQRLVKNLRPAVSRVGAARDVKVPTYVAPHLVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9D6</accession>
    <name>A0ABQ5S9D6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000034">
        <property type="protein sequence ID" value="GLI66542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="143" mass="15290" checksum="CE823D6553407620" modified="2025-10-08" version="1" fragment="single">QLEKLRSGAAMMEARKGGLSGASTDLQGSSITAKFAIIPAASCPAMGATSPPSAVEVADAGAASIFINKAAQQPQELYQPLELTQPPKPHPKTPERRLPPEARNWGLQDCRSWAGEQHWSSWAEVQAFTKTLEPTLAEAIREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9L1</accession>
    <name>A0ABQ5S9L1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enhancer of polycomb-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009886</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enhancer of polycomb-like N-terminal" evidence="2">
        <location>
            <begin position="68"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1130"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1214"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="535"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="707"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="739"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="818"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="829"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="863"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="922"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="953"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1132"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1240"/>
            <end position="1260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1260" mass="132742" checksum="61975418DBE7DC70" modified="2025-10-08" version="1">MIGRTIRPRPLSYTERIPVIFLHRPKHPDDDSPPIDPALEEFLRNCEDERERDQNGLLRGLETSRRRRKNRHHRQQQQQAEGKTKATAGIQRSAAGPSGPSARSDPHQTQLQQQQIDVAAAGSVTASLAIAVPTWREISDFASNEEAMARADTRKAAEAGPSVPLLAGILPDVSPCATTQSNMGLDAAAYIIYQDLVRDRRAEPALLQYDMDETDEKALRAINTRRAPPPKPGGGGSGNSRTPPTTRAVTAAAAAAAAAAAAAILDEDSFEALLGALERAHFEALQQRKELWQAEVKAGKVPKLPSPVKILPWEAAVAAVKLHQPPLPALPRALYNHWLKRREEEGGPLLEYLWYEQPWKAICFQDREPGHEGEQGDMPFMATETKNSTRSAWNRIRINKREAYARLMEVRDEFELLRTTADQVRKRERLKKHLLKLYRAELAAKLGLNFHSKHNIGAAAAAATGTTGIGGGNEVPGGPNSAMAGTGGRGRGAASAAVKIAAGETEVTATLAPLQRRGRASEPPPNKRRRVSTDAPPAAAAGAPRRASRKSEGALAADAQTIPTTAAAVKAKMSKPRQGAEAHGTKKAASPPPLPSSSTSTSSAVSESRSSSLSHDSSSSGSDSDLEATEAATVASRGRKPPAGSGVGARVRGQSRATEGSRPPTRLRPGGRMEAAAAVDCVTEDKSTASTGGDSSSLVEGEESDGSDGPTSSSSSSLSPGSSSSLLSDGGDEAEDSGSDGTSSRSGGSASGIDVESEGGGETSSSSSEEEEDGQKERQATGHKRDRSRNQSGAHCTGRVKEARRGCTGGRSILPYDDGTEEDEMEVDGNEKTETEQQEAGFEHKRNSGGRKWQQGSDDIRRKSGARGRIARISKAQETQQQKRQPPLPQKRRSTSRVQTRSVAASQLTGAVEAAVATRTAPSHTVSSSPVPSSSTTRSSSPTRSDCGRRQNPLSSSPEPSRSTSRSPSPQKGRRNSAPIADIARGKRGAIAQGARSRRLTITAITERPAVRHRVRFSTKPANVVLARGSGSSKGSNVDPALKAPRPNRELLALLATQHSGVSMAAAAAGGSAPAGVAADKLSRVHTAGARAALAPEHLANGGAPPAGAAIGAATQPKCPQARLSALVEEQATGRRADTSSRKVHHARGAAPSLQSLPDEGKRPLEHQSGTAGAAAHSMRVGSASSGRKKTKTGWRKELAALLGKPFAVTTRFWLQRNQAQQPTKGREGSGGGGSGADGDATRESSRRMSMLRERNSTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9N7</accession>
    <name>A0ABQ5S9N7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_009871</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF9">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="564"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="706"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="229"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="306"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="808" mass="83244" checksum="2D9603D46E38CB78" modified="2025-10-08" version="1">MEALCSRLRELAQDCIDKHLAASAIFYADKLVTFSNNSPGDIYLLAQAYFAARHFHRALSLLRNAGAVDLGGEFTYLAGCCLAEAGDWEEVIALLGDDETIQEPQACEGSVLGTAHGHGGGGAGAGVRVQAGMCCLRGRAFEALENRGRATVWYQTALQLDPYCYDAFAALIDGHLLSNEAEVALVERLSGGMVEGDRWLGLLLRAKCKKYVQPEFIEAALRELEAPAPEQLAEAGPGPASHQQPYAPPSGPLHTAASVGGGSGGGAAGGTGANAFPILFMTPPRAPSRSGGGSGGGHAPAPPGGADIGGSGGGAGAGSGGGGGEGSGSGTRRGARGGSRRGTRGSTAAAVPTSQDMTAGLRRDEGIDDASVRTTPRPHDHPHHHSPQPFHVAAAGEQPPPKGATQPHAAESRGAAGKARSMSPGPPPPQSNGVYGGGRVSSGGGPFGCGLSGNLDVIACRAELLFHRGDYEAAYALTRPVLCCGRDPYALQLLPVHLAAATQLAAYGSEARSDLFLLGHRLTEEHPELAVSWYAVGCYYLAARQPEAARRYLGKATQLQKGFAPAWLAYGHAFSAQDERDQAMSAYRTAARLFPGLHNPHLGLGAEYCATANLPLAERALLAAYDICPNDPAVCHELGVLMYKCGQTAAAAMWLDRALQLLPGGRPTVHWEATLVALGHCMRKLCRFNVAAECYSAALALAPASPGTLAALGYVAQLAGDPRVAVEHYHAALALRPDDPFATDMLRLALQEFADAAAAAEAAEGKEAEAAVAAAAAEAVAKTVNPVAATPVPAVATAVAPQAVPSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAK5</accession>
    <name>A0ABQ5SAK5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000051">
        <property type="protein sequence ID" value="GLI66930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF190">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="966" mass="100331" checksum="1F410C92F6515BEB" modified="2025-10-08" version="1" fragment="single">HNHISPSFAPSLGTDSSWDRVPLSFLRTAVIMDSDLERCNWNLTNGQCGAWNGSQLGIDALHLSERAFQRGSNGPDWTALPEGVLLNISRFLNPHTVSNARAVCSPWRGAFSLKVHTARLEISEHRAAQARAFIRAFIRAFPSTTEIVLDITFRRTAREKIQAALEEHFLALQDFGFQALQLNLDLNSFNERKIKLLDVSLLAAWNGLVRLTLQESGWRSQQLRVDAATVFSSLPALQELSVVCKYSGQAKAKWWRPPTHAERLAARMPTWPPAAAAAPAAAAVVAAGLTDSTAAAVVAAAAVAKGRGAGGVTSRGDLLRPGLGPDGLGRATGMVLLPGQLNVVDEVMEDAAGPVRLQLPPPLPPSQRAGIGTGMSVCPVVTQHLRCQMTQNQSMSAELQKTLKRITPTLQSHVSALTFEIVEETYAEPSRCATPLELSALPGLKSLTVLSGAEDALSHSKLDLVGATGLETLVLFDVSCGCWCYKKPGHGIPPRLLSAPPAYLRSLTVVGFVVNQCQNLHALSSLTCLEELRLEQPDPDCLAAVGGELLLTWLPTSLRKLKLHSVVLPPCGVPMVALTDVQTGSAGISLQPAHHALYVGGSVAAASGLATAMAMAPSALAPTASFASHPQLSLGAGMPAWLGGGGNGTAAVAGGGAAAQFLYGVAPVAPLPQLRELYLSKCKVPTLGMLGVCSQLTALGVINCLSCSGMLPVTAAMPALRFLAVIYTLAPEQEHGGGGGALSNMAPAAFTTADPVPAAMTACSGFNGYTSAPGGFTAAAAAAGITVTAGALGCNGAGCSTGSADLAGAAATVMAPAAASALPLHVSGLPLPPVVWGSDEQVAAVAACSSVTSMELILPRGSAGANAVAALASMPALMELDVSLPCGLSECILGLARLSRSSGGRLRRLTLWVDGRTCVNRTKAQQLQAIQRSLQAHMPDTLVEWAGAESRQGSWTGLEARVFNPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAP7</accession>
    <name>A0ABQ5SAP7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000039">
        <property type="protein sequence ID" value="GLI66729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="711"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="753"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="799"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="598"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="609"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="652"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="919" mass="96978" checksum="16B4E89B9C2C6D61" modified="2025-10-08" version="1">MSICISAASDGPITPGHRAGCHSEHSEVSYIPVSDALEELSLRHASDKQLRSQEGGREAHSMGDGRAFDSQSWFSHQLQKLGASWPVPVAAVASILVFTCMGYTWAQARPGAQTILLTSLLQFCWAVMSSQEPRRFLRFAQLASSFLGILVILPKRDVYGTRIWAAAFDEDGGVILPGFATSLAKLFGLLDTKDTGDNGYALADDADLARALLRLEVSLAVMVVIWLLQTVAASYQHATRGGTAPAIGGGSTGFTSAKQSACKSHFDLPALDIQTPCCVPELWLRNNTRAHNMIDVRREIGIHSIYVAPIYGLSSTGHPDHCDAELQQQLSKASVAATYIQRVLLLGACLAQCTVAVIIIGSWDALAGRLDAIEEGVSATRELENLVPSADSRGTSWVSLEILCIAMSAPLALPSLLRSRLLLHTAIQLRPTAGRSLGLLLLDVNAAKLLLSTSVGVLGGWMSGALAWQLSQVVTMLFGVMKLCLDGFKAQSQLVYNVPMGGSGITPVEAPARLMLVVPEGPSCEDAHLGFISIPECPSARALSPLFTDSIGRLGGGLLDRGSSGRSNIDGGPVALATGNCGDAGCTITIASGNTMVSSSGNTMRSPYSSGDSLASAADADQHGQPVGGSTGAACTTPGAMTPHLARRPSLANGSNSPHCQQQHLNMPPASPACTGNIASARPSLSNRTFPSVIYSRGCGRGVGALHSWDLLLGSLGLLTCMLWVALFAPPIALLGDEAVEFFEGLMDTGIGAIATLAMLTSIATLSTVAGTCLVWCIASGELRQRRRLLPIESRRWECLTWACGLPLGVAAFSALSWALGDNRNAVAYLAAEILRCTSLWPLIWLQEPWLIGGVDVVGGCVQHAGCGVTCCILRWCGLRPSYCGVPGGRCDERWTGPGQQVVRVAVRRARWPVAVDVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAS7</accession>
    <name>A0ABQ5SAS7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010458</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="157" mass="17008" checksum="BD0EEB8AC15CC62A" modified="2025-10-08" version="1" fragment="single">LRDSMIMATSPAIITVITYGQLVLHQNPQSKAYLGDWVTLVDDARRGGILDVIFVYEPEKCRRMLEELCREGSSGLWKAVVRVPYNFKNRLENNSIRNPAGSGRVRLHGGGRGLTTIEDVVWDRGQHVGCDTTSGGGRCGAIGASAVLDRLGAPEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB02</accession>
    <name>A0ABQ5SB02_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010598</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="305"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="381" mass="42766" checksum="D12F4B813245DC02" modified="2025-10-08" version="1">MQIFVKTLTGKTITLEVESSDTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCK4</accession>
    <name>A0ABQ5SCK4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000047">
        <property type="protein sequence ID" value="GLI66906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046338967" evidence="1">
        <location>
            <begin position="36"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="128" mass="14333" checksum="8630827F5983B368" modified="2025-10-08" version="1">MYNCLYDDYAFSIVKAMKRVVLAVLLAVIVGTASATDPDQNSWSCLLRISRKCNGTYATLFESKYYSNNVTCKTNVKLALEAYNKAACPDTNRALMWTCISGNDFRTGQTNKALINDWTAFLNEAVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCN0</accession>
    <name>A0ABQ5SCN0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="350"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="541" mass="55709" checksum="3BF0BDAC7ECDDC90" modified="2025-10-08" version="1" fragment="single">DEHRPLQRHLYGLLAAVPLCAANGGGGGGGGGGGGAAAAANGGGAAGVIAERGGGACGCALSTAGCSQVIPSFAFSRRLTAPRLYWGAQYQAQVPPPPPCGSAAAAAALQRRPPSAAALARMGVPLHAAQPLSAAAAAVAARTDFETAEAVRAARAALRTTKVRGGGRSTLAAAAAAAAAAAAVAASKAEAASPPSANPDLSPPVKMEVDEQFVVGTRYDSDPQLDVRDGEAANGQGESVGMTSVGTGSPSGLKIVEAEEQAQKREQEQAQGQEQGQGRQMKQQEQSADGDGDVSGPEAGILQREQRGQEPDVLNGPPEGVQQQETTPTPMLEHQQPKMQGVGSSCATEQQTNQQQPHHNQQQQPQGKGQGHAQEEEQREEQEAPEPGTILFPADGGALGAEVRRQLEAAPDMTARHAVMDAALRHVDDTLGNMARVLGVDSMGARVRGLWSTEAEEAFAGLMGQRDTKLGHLIRGAHRPNLQHDAAGVVSYYYNVWKTRATREADAWHQERERQRDRESGSQLSSETEGGGGGSGGAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCW1</accession>
    <name>A0ABQ5SCW1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">VaNZ11_011698</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF68">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0243">Dynein</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="1512"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2143"/>
            <end position="2295"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2497"/>
            <end position="2654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="79"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="481"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="659"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="875"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="2962"/>
            <end position="3017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="501"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="675"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GLI67481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="4191" mass="470588" checksum="2C45F2019DB39758" modified="2025-10-08" version="1">[encoded data, 4191 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDR8</accession>
    <name>A0ABQ5SDR8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="951"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1008"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1029"/>
            <end position="1041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1074" mass="108306" checksum="D9EF8D0B3C21E8C2" modified="2025-10-08" version="1">MITKPAVQPNENNTDKRTSLDASSTPLEPPQASSGRWSVQAAEAAIIAAVQRRFSRIGAAQWPQSPDHRATTVITSPLSPRLSAAANPERCGDPASPSRSVLTAGGVPDLNASMGRTSQAQEPCGNRKPGAAAAELPVDMSKGGGCSAATTIVAASTNPPPLYGNSEILPNAASSAPFCESVSIFITPTGAQADDYVPKTANDTDAATGFSLGTPVPAPGLKPPPGPDPVLLPGIHLSCGPELQTADFAGLMTTAAACAQASWSALSGSSSAGEATLDCLWTGDCSAAAHVISALPPQRRRRLHHQQREDDSHMGSFGVHHGGGGGQEAAGRGSEDAASDSPKRYGPNGGAGPLGTYSIGTCCPAAVGRYGTLMISSAGWRCTELYDMHDSLLLSRLSARRVVAGGLVGGAGGGGDDGNLAGIGTSTRMGLSWAVDSAFTKGDSKPQDGDLQAVGVPAGEQSAVGKEEAEDEAEEEAAAAAGRSPGPSSGMAGKSQVLISHLNRRPYEVTPTVSRTDQNSRTDPTSCHPADAVQARCGPFGGLPSAPRVQAQRPGPASTPLPQLLQLHLPAQTHVQHRLAAANFYYSGGGSCGGGGGGHGGGAAPMGGWGAALEAMLEGVAPRADYYSPPVAPLAPAANGWRTPGANSAARSAAGSIRPSNAGAMVERSGRGTMMASMRRLAGQIKSRVSSTDVSGIMEVAPSDRSSHLSIHSGGASATANPGGAGSAGTMYHRGLLCSTSSLSHQPANGATLSLDVNAASVQSDAVFGAELAFQKSLGVALPRAPCKLGSGHSLTQGSMGCSPDSCQGTTLCASAASAAGRAATAPATPMPVGTFGADYWQEGMRSKVSYTLDAFGEREARASPEEGPAAGRAGAAAATETDFNVGYVGGEPITAVTTLSVRQADDVDDGCVGGSHAAQCRTTFYRNSSYTSRGGAAKDDETPVAVVRGASKSAKSHQVPLPCKQPSASEVKGSGNSEQGDAGGCGGRVFYRLICLLGAPKKRLFKSSRARRSRSTTHGLSSWSGSVRGSGGGGGGGGGTLATRLPTSDIVEEASRRDRLARIAPLAAAVFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE97</accession>
    <name>A0ABQ5SE97_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012606</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="287"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="320"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="363"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="408"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="190"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="552"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="590"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="685"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="870"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1061"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="628"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="722"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="762"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="790"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="873"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="937"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="976"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1103"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1117" mass="113277" checksum="523C9E9260D06F71" modified="2025-10-08" version="1" fragment="single">MMFVMCGLLGCLSQAFKRKASTTPSGANEHPNHHHGDRYHNGDRNHGRSAQPAIIMGMSVGSAHPTASNLPVVQATVVERHPASPGPLNMTAALPAPTPPFEADPHGGAVASTSAIAAATQPATLVKLERGAPSRRTPLHVAAERGQLHVLSAIIENLCCSATAALLEEDSNMSGSGRGGIDDAAQLRGGPGYCGNGDRGDGGGGDSFTTYMRSSATENVEGGASGRQQQQHRQRGSGHRLGSGQPQHYRSNVSMVEEFPIARPGAGGAPLAPEQYIQFHLNMQDAEGITALSLACRYGHVDCCRLLLANGSARTLSDARGNTPLHYAALRGHLGVLFMLLDEFMEPGQEEEFNNYVDVRNHAGCTPLHYAVWGRQSGSVQVLLQHGADVMPRNSLPAQELTPPVMVVGSTPLHLAAARGHVEICRILLKTFAERVLEPLQLSEDDPQPPTLASLDPRVQANGNGHMPYQVAQRLGFYPLSMLLRPSIPILRLFSDEERAVRFYGPAPLRAIAAEALNKKLLTELDTLAAAVVAQTTVVGVRENAAELVRQQPAPTAAGNGGAGASNAGDAGTGLQRPVSAPATLEQLAAAAAVDAEEEASGGSGGDRDADATAGRYQRHHSLVADESSATATTTAVPRTSRTSRLSPSASPAADGGAATSSTVTGTGQRATPLRQLTVEIAVAGGTLDNDGRPSGLARRPLVSEVPSPGPAASPPGSAGAPPVPPRTPPFGTPIAAQVLSPSGVSPRLMSPITRTPPSPSTGGGPSFGSGGSSGASPLAIQHPSPPQLLLTSRGSSARRSTPPLTSNRSSNAMLPAAAPLVAALSRPPSAPRANGLPAESSRGQLMLSLPGQPDAPELQAVRILPAREQTNGEPVSSSASAVEPAAEASGSRPTGDGSGVALTAGGGAPVDGGAVTAYSPRTPARQPSAGTLHARSPTGSPTGASATSTSLGITQGSGTGGVNGSITGSPGTRPASSSLATMSSSSLTVMLEVRTSGTRRSNDGGRPPGLVRLRSNPLFEDVHGVNSMAAASLSTPAGGSGSGGGVIPTAATLSSMPPVAEVASEGEEGAPSEGGPGRLTCASVTPFVSRLSGTGAGDDRSSGNGAPTTTMETVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SED1</accession>
    <name>A0ABQ5SED1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012638</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF533">
        <property type="entry name" value="HSP70-HSP90 ORGANIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="139"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="207"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="68"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="284"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="338" mass="36376" checksum="00555D478C39F2AB" modified="2025-10-08" version="1">MGKRRGQGEPREDQYGDFGNSQDKQHFSGFSSGKGLTGGGSAGGSRKGGDVTFQRQMPKFLQKYSHLMGKTAADEEEPVVFGGDGKEGAVGQGPNAEEEAGEEEENIEVEALKRAMADNPELAREFEGTLASKLKQAEATEEKERGNALFGRKMYDEAIKSFTRCIELDPSCEVYYSNRSAALAALERWDEAAVDARRVLAMKPNWAKGWARLGAACMGLKLYGDAREAYEKALRIEPDDQVLRRACEKAETMELQQMREGKHTFKKMRTGGATAAATAAAAAATATVSSGLVQGNLRDPPPPGGGGGGRGAGGDTHRPRSNSNKKILSFGDEEDDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEK6</accession>
    <name>A0ABQ5SEK6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_012754</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="267"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="603" mass="65247" checksum="3984FB0A21C86B66" modified="2025-10-08" version="1">MSTRGREMDAKLAGSRSFVGTLKRLFMASHRSEDGAKVGEATSPSQTPTRAPHITSPEAGRDSLDIQLTNASEANGTFPSPFSSRKSLDRQLFSSNSGPLISTFQRNISVAKAPLHHSISDSCENSSVLTNSQRLLTDDAGWPFEQPQKQLQSHLGRGARGSPLKLAARADASLPSLYDSPSRTSLDVQSVAISSPALTGASARPKHVLPVPLPSVEAPAQREGHQPPNGAVDMSAGVSPSPTTTLLALCPGATPAMRRRHWSLEDYEITRRIYKGSSSAVYKATCRRSGIPVALKVYSLTRVPPNVLHMIVREIKIHVELVHKHIVMLYGAFQDEKRLVLVQEYAGRGDMYGIYRSLNRRMTEAQLTHLVLAPFMDALSYLHGRGICHRDIKPENILFSNDWRLMIADFGVSIDLNQERAVTRAGTLEYMAPEVERCPLKMLPEENKENKDLAYTTAVDIWATGVLAYELMVGFPPFVVDNATPAGGAGPGAGASGVSTGRGGMFLAEYANRRTLSFPASTSSHAREFISLALAERPEERPTSLQLSRHPWLAAAMQQPASRRSLGQLNSFNVVPMQPSRLGSPSMASGPNSSAVAAAALAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEN6</accession>
    <name>A0ABQ5SEN6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_012239</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47444">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47444:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="72"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="89"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="143" mass="15655" checksum="E9833B94227C5223" modified="2025-10-08" version="1">MGGIGKRKVGGDHKHRKTFKQQRRSQFQERHIDQVWEDVRKPPSLVHNGKTGPLGTTSTAELDEDVPGFGKHYCIPCGKYFNSATALASHEGERPHKRRVKLLNTTPRPHNQRDAEAAAGMGAPDNGPKLRVGAAAGVAVMDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEQ8</accession>
    <name>A0ABQ5SEQ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012477</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009959">
        <property type="entry name" value="Cyclase_SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07366">
        <property type="entry name" value="SnoaL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="184" mass="21312" checksum="72D5FBB874DF61AA" modified="2025-10-08" version="1" fragment="single">EQYPMMRSFNSHHSRTLRRFASARCTARNTDATLSRQHIALERARAYYEDVWSQGRLLKLNKIMAEAHEQYDMVWQRNRAGLGRKTMMRGILAYRSAYPDLAFRVQDITYSEQTHSVFVAWTARGTNLGFIRDQPPTQEVTEFSGISHLIFDDGNQIKASFVYRQASAGEERYFMGDNRSVLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFH5</accession>
    <name>A0ABQ5SFH5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="410" mass="42957" checksum="B483FB32DB883CD0" modified="2025-10-08" version="1">MVQFLYIAFQDALRRKALRGDVVPRWLAAAVAIPWVCVLSSAVALAGWSLWVVKSEAARQSTHNIVKGFGLSLTREPWPLWAPVPVSCVLLPVLLVLLLSAWARERIQRRVALGGTPFSDSATSESGSESASYTPSTRRGMRPQEYFATAGTAATATWLLHAWLVTVLVCMGVWAAAVVTLHTATKAVMPYRSALKSTTAAVGESGLKNLVLERLNSDVTARSAAVATRGSSSATGLAGLNWGPHGDGDGWGPGGCPVTCVDLSALSYYLGDETCCCDTIGGIFAAEAHSAAASHSLRLALIGLGLLWASLSWLLVAAGCQFAHTESEAMLCWGLSSPCAGALIQPQLQRGELSRQASVQRLSSWLARVMGIVDVSGGDGGRGGGGSGEEVTAAVRDGSREPLLDSNNTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFK6</accession>
    <name>A0ABQ5SFK6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013172</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF80">
        <property type="entry name" value="IP06716P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="4">
        <location>
            <begin position="2"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="197" mass="21824" checksum="E76590EF8F674DF0" modified="2025-10-08" version="1">MAPVVLNIYDLAPQNSWTYWCGVGIFHSGVEVFGVEYAYGGHDYDYSGVFATNPRDAPGQVVYRESIPMGETDLTQQEIHHLVQRMGNEYKGNNYHLLQRNCNHFANDLCRQLVGKEAPTWINRLAGIAVMLHCLIPTSWVPPLQTPSAAPILGDTAVIRREEDKALLSPAGQRSDVYGERLQPAAMLPSVASGTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFQ5</accession>
    <name>A0ABQ5SFQ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013263</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF6">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="149"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="341" mass="34501" checksum="C2215745A662C685" modified="2025-10-08" version="1">MYGYATGYGYGASGYGGGGGGYGGGGGYGGGGRGYGGGGGRGYGGGGGRGYGGGGGPRSDGGGDDAYQEGKLIFPGLSEHTTKEGLEQYCSQWGQVEECTVAGGEGVVRFSDPTCAQSLLDSEHVIDGAKAEPRAALPRDKAGTGERCQKIFVGGTGDITDEELRNHFSQYGEIQDSVIMRKDGVTRGFGFVTFADPISVEKVLVMRQIIRDKTVDCKRATPREITPSAGGGGGRGGGRGDRGFYGGGYSGSYGGGRGSSGYGRGGAAAAAAGSYEEGIGDGMGGYGMSGYGGMASMAYAPAMAYGGYGGYPMAYGVMGYGGYGRQTMARNSVGQQRFRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFT1</accession>
    <name>A0ABQ5SFT1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012688</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010297">
        <property type="entry name" value="DUF900_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36513">
        <property type="entry name" value="ABC TRANSMEMBRANE TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36513:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05990">
        <property type="entry name" value="DUF900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="540"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="767"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="964"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1171"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1293"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1327"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1480"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="560"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="787"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="861"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="889"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="993"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1022"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1299"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1349"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1399"/>
            <end position="1415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1633" mass="170672" checksum="A7BC76AA24613936" modified="2025-10-08" version="1">MEEVSRAPAGQAHHAVPDSPVVLNLNIVLPHMQYRKKLRFFNGIYTPSNVSRVFFMWLVIFVLWTFQSGFIERTTKSYDFGVVLSLSYSFPNGLTPGSTNAIILNIITYMLMAFMLIMVAVQVNRYDPFMQALGVACVVLFGVVLSRRSHMPADVAQDGKTLSGGFFLTQDLVMQIFVWVGLAYMLALFAVRFATPMAVRRNWLLFRCGITQRHDLTDPAAVASLIDFPPGYSLTTERRLVACVIRYISPWYAPMMGHHESVTYVGEVDAAGNPTGYGEWRDTHWHGEHLTGFWHRGIPTGPFRSRETGSTSGFVNLRIAYFRNRGEALRKAACLPQLKPLQFGIVACEVSTSGAWFKSLPRLRELLPPVDIAEGLIAEEEAAIVEGRIAPAASHRAGLAPRTKSAQTCAPQPLPRLCTPRRSTYSGPDASSTGVSALFSRRLPVHIAMRGPDAGQQSRLAAASYPDAETPLELQQPGGGDGAVKGTPVEPAAAAPTSTSDNVGAVSGGNVPKDTSQGASGVPVTAATAACPEPTLTLALEQGNSPVSSAKLRSGRSLDSATRSAATTTTSTAYTGTTPTTATIAVTTSTATTAIVATAATAATAITDITATSAPTSTGPGRSHSAVLVYPVSGSAPSQVQRGPPSTRIPTLARLKSALSGRAHDGVGLEAGNAPPASSCERGAPLRNLSGFLQAEMALQAPSLRHPGGAAGITLQPSITTLQAAAGERIAAPSAPADAAPTASVFFNGMGHVPRLVSMLMPMQPAQTTTVHRRNPLRQSPPLVVGQGAGPDRGSTGGSGRAAGADTERKNSRWGPGWGSETDVGLRHRPAGAAGAAGLPPSITKGSSQRLRWRLRSGFRAGASPKYGSSSSASERTHYGRRSASPQHPGSMAPGASVASPSGPAEAAAAAAAEDGDGPVYHRALISFIRSSQPFAVVGGHGSAAAVGTHGSSWPEGAACSGGVARSGHGSLQPIDESRTSTCGSGPELRTPTHGSPSGSMHMNAPSERSSNCQRDESLQQGTTAAGKATPATFGDMPEMKRREARPPPLNFTFDRPPEAVLFVHGLGVDLHMALRHYAQTMALIRFPPYITPVVFSWPSSIPVAYFWARYRGAEPPATGLSLAAAVRALAADGFAGLHIMVHSMGTRVLANALPHLEAILGEDGAATTTTTAAGNGGNTGSEGYSCGPDASGNGSGNGSGDTSTRMKLRTVTICNPDFGLKRFVEDIGPRLRALCPHVTVYGDTADEALGLSEVVNALARPFVNAGAGIADAGNTVPALVSEGGVSAVHRDLGEQDKQPVQQRASNASEMLRDTSAAVVRAAAAAVKKTQSGSPDEPLAAAESVSELPAPPPPPPPLPAPAPAPPPPPPKSAQVPSPPQPGRRTNLPPPLLLEMQAPQPQPQPQPQPQPQPQPQPAQLEILEGMGQGAGDTLGAAKVAAVLRSNVAALTAGEAGEATGLEQPNGGGSSAATAVCPPAGRQVRDQMDHGAASESGHGSGHGGVAASAITGAVNRSVADLSWWSEQVVNYRCRLHKPPLYDWLEHSLGRRIYQIQDPTTELPLDIDVIDLSYLSSNVSSIRHTHFALNREILDDLWEVIVLDRRASQRKSRLEHVTGNVYSIAVAPSFLSTQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG58</accession>
    <name>A0ABQ5SG58_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013388</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="43"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="455"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="491" mass="52259" checksum="126ECD7FDCF3595F" modified="2025-10-08" version="1" fragment="single">MQLSGSIPQQECENDSATGTSVRSLSETMFGIHGNKIAGKYDIKKKIELYKGSISTVYKCLLTGGKPVVIKTYHKAKMAEKHFHKLGREIAAMRALCREVGKKGVVQLLDTFDDTSGIYIVMECCEGGDLFKRLMLHGGKLPEQWVCVEVIAPLLRVLERMHELHIMHRDIKPENIFITAEGHVKLGDFGLAIDWTKELPFSRSGTLDYMAPEVLMNPATHTQESPEITIAQLQSKNIRPYTAAVDVWAVGCLAYELVCGRPPFEVEDEKQTASLIIYSNTIHFDPMATPAWADFVTQALVKDPRIRPTAAALLSHAWIRTNLERAATVPAAVGPAALGAGLFGRFSSTPRTPEGTDMQPTSDRSGMLRASPRAAFTAAAASGSGISACGTAPAIGDGSSVGTGATTSCVISAGGSSGATGINSPVAHQQQMQSQRVCTPSNTAQGFRTAAAMLATRGSQQSPSTPPSQPSTTSTSATTTPVTGSGTIYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGJ6</accession>
    <name>A0ABQ5SGJ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013632</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="171" mass="20089" checksum="93F62CA60653BE1C" modified="2025-10-08" version="1">MEKDREKDRERDDEKEFDRWKKEFAQIPEIILREYWEAFKLFDKDGNYLIIKEELATVMRGLGQTPTEEEICLMMTEVDKDGSGYVDFGEFVGLMTRQPSPVDPEEDLRKAFRMFDRDGNGQINAAELKHIMTNIGETLTEEQVDDMIQEADIDQDGMINYEEFIRVVATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHM3</accession>
    <name>A0ABQ5SHM3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="3 6">1.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013465</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Plastid</location>
        </subcellularLocation>
        <text evidence="6">And non-photosynthetic plastids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3 6"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05333">
        <property type="entry name" value="BKR_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011284">
        <property type="entry name" value="3oxo_ACP_reduc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01830">
        <property type="entry name" value="3oxo_ACP_reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009466">
        <property type="entry name" value="PRK12826.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF2">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="7">
        <location>
            <begin position="81"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="323" mass="33272" checksum="78884F426E1D0A90" modified="2025-10-08" version="1">MLASKQSQATARSVRAKEPVMAAALRASAGSAVRPFTVASSAAMHRLGVNTGCVARTPRSGRGRSAVSVVAMSGDGQAVKPVCLVTGASRGIGKAIALALGAQGARVAINYASSAASAEEVAAAVVAAGGEAMVVGANVAKREEIERMFKEVSDKWGRLDVLVNNAGITRDTLMMRMKPEQWDAVIDTNLSGVFYCTQAATKVMGKQRSGRIVNITSVVGLVGNAGQANYAAAKAGVLGLTKTTAREWAPRGITANAVAPGFIASDMTAAIDKKYEEGILKGIPLGRYGQPEEVAGLVRFLALDPAAAYITGQVYNVDGGMVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHM7</accession>
    <name>A0ABQ5SHM7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF18">
        <property type="entry name" value="ORC1-LIKE AAA ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="883"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1124"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1284"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1339"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1443"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1647"/>
            <end position="1680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1710"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1822"/>
            <end position="1851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="883"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="897"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1284"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1343"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1461"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1715"/>
            <end position="1725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1726"/>
            <end position="1757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1764"/>
            <end position="1784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1792"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1823"/>
            <end position="1833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1851" mass="190623" checksum="834137A2BC100F31" modified="2025-10-08" version="1">MEANRPKNPDEGKVAALAEVDAQRATLSMRNAYSTAAKVTMPHGCSSGDTDAATPTQGPEPHPGRFEHATPLATGVGGPTAAAASRRLWQQMQCDDGGSEEEEEYGGTVPLSGGEDEGGCGGGGAAAAIATAPPKLDVQSPQQRDAASPMAIDVPAPTTVAAGEAEAAASPLAGVAVSPGDAASPSGGGGASTAEAGSTGSKKNKSKGKKKGKKGLDMDLDLPDLDSEPDLDSDPELEHEADDDDGDGGDDDTEDEDADDGADGRQSVAPTAVFTGPWNPTAVRLRPSDREPQVVSGGGGGSGGAAGGASDPGNSYNSSKLCRSPSDVRDTFGDVMTSTSDDDETQRVIAPGGQHRRSIQKLSAAAGRASRAAAVAMERPDSGGGAAAATGAAADPKPSVADLDAPGLWADDEDYIYKDVDPNDLDIGIEFPQMDYKAEDLELPPDDFWAEGEGDEVVMGEDGKPVRRVRLSAAALAEGEAFIGLEGLVSWGRDGTFRGTITRFDPNRTRLMWLMSYEDGDEEWGVVTEDRSGWRVHGGLRPVSWIGHPPPPTDPLNIAAAAADAIPAPVTAAVQRQTAVQAAPTFVLNLMGAEIEVAVGRDGQTRFGSIVDFDPNRPRYKWYIRYVGYAEPAYGGTGEWGCLDDGKTTWHTHGYKRPVMSVSGCAGDLVPVAPSKPTAAAVAAAAAANTFTPRKGPFGRTGLGLLYLISASEPEPDGKYRVPVMALGSIRGAYGSAAGAAAAGKGAAAAGLALGPPPPGKPERVSSMAMAGVPDEYGATLVGAKITITVGRGQGHKRSGTITEYDANRPKYRWRLTFDNVGEPAHGGAAEWGMLSDDQSLWLAHGYKRNITALSGVPERKSRPADPPQPLVAVAAVASPALLPPRPMPSPRPAVAPTPDADGAAAALPDGTDADGRQRSPTEGPETSAGGAATAAAAAGDEKLKVEGADAIDSELDATLAAVAEAAVAIMFDGNLEPAAKLGIETAELDLGVCEVEELGTNLIGARLLVSVGRGGARRSGTITDYEPNRPRYKWRLVYDPEVLPVGINASANANTSAAATPGPAATPTADGAASAAAAAGTPSAAGDAPAAAGAGTGTTADGAGSRVEEAADASAGAAATGAAATDGASGAGGSAAGPATEATGTGAPYGAVVEGEWGTLSDDRTAWLCHGYRRAILASEGVPVLPPAYITAAAVAAAAIAAAQSPAARKRRPGFIDEQSAASALLTLGKRARREAKAAATAAREARQAEIAAAGGHVILVQQPATAGDGAAVAPSGGGDAIAPGAATPAAPAANNDGYTPGGEEDADRVKRVKIEPGTGGEVGVTYIRMPLSKVNSPFGPTGMGGGGGGDGGGGNGGDGAANVGPVSPIANPEGVVGLEIEVDRGDGKHRGRVLSYDAKKPRLRWLVRYHDGNQEWGTLADDLSTLRVYGFVRQITWTSRPPAVLPKGTKGGNTRHRSSSIARASPAAPVGGSAAAGGAGGGAAGAQLSGGGAALIRTSARRLSENGDSDWVGGTGSGYGSGGTDYRYIFRRNGMCRAQITHQGCAIISPYYDTPEAAAAAADFYNYKLRGTAAHLNLGLTAEQRAVLDHLTLEQLQSYIKGRGDIHDLSKALLQYAGEAGQRLHHQLQQHQKMIQELQRQQQQLLQQQGSQPEQQQQQQQQQQQQQGQGQGQRQDGQQQQQQQQLLQQHLQQHLQRHIQLQQQQLLLQRQQQQHHHHHHHHQQQQQQQQQQQQQRPQQQSEQHQQKGQQHQRQLSVEDPEAMTAAAMAATAAAGYSRAASADPHDTPDTVPPPVVPPPPPPPAEALDYPEEVQIQVEVKVDDDDTDEDPTAADGVGPWDEVMADIGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI87</accession>
    <name>A0ABQ5SI87_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="5">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014111</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="5">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD/DEAH-box helicase" evidence="7">
        <location>
            <begin position="369"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="773"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="92"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="183"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="502"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="547"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="674"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1103"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1139"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="136"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="517"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="693"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1154"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1198"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1223"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1235" mass="130873" checksum="F27109857227A3E8" modified="2025-10-08" version="1" fragment="single">MYGVVRRHGWFGIVAPSTLPDLSSAQSRFPGKQLIELSIHHTDQQSYSGLRPSALHAHLSGMPGRLPRAATPAPCNFGTKNPNLSLRSAFHRRAKRRKNVSSADDTDNPDMWLGYPPAVASQSSPGLGSLRGEGLGSLSSSTPSFESPLSPSTARALARGFREGELALASAGPLQVWHCGSRSGGLWKTRPPAQQNQRVHKAKSTGPPQSLQGPQWREPQAPVPHPWQPLRSPSAEPHPDALRHSAGHTKPSPPSSSATPMIQNPSLNAIEVCRLPSTASLLPSESLLGPSSDHVTPGAHAQLPGGDKNDEPASTIVTHAVGSGSLATPSAGADARASPGGGSGAVNDGDNIDVVLLGPAGRPRDLSVLVAAVERLASQPAGRGCIGCVVVSPSRERVDALHEVAFTLISERGGGPEAMHVMAVHGGTRFREDVSGLRSQSPDFLIATPGRLIDLLTTDEVELSPIFKSLRVLVLDGGEVLASRSFRPQMERLLRLLPNTALYTPPRRRGPDRLRPTAKTGTGEGAGAAQAQLAAPRAVTLLAAAGGIPHPTERRPPPSPPLQSPAAEFRGAAAAAARMYPGNPVLGQGRQPRVGWMTAGSGGAATQSGMLPHPELQSLIDLALRYGVAIFKLSSVGAGVGGEDGHWYDTAVSNSDLGPVRTLSATVIAALGSGNTQREGQDMDSDVMGQGVSHGSTNKAKSSSQVVAQPLDLTEREAASEAVPSTSSVLKSDPVFVDSPTPEKVAPPPLVPLHVLAVPYEEHLPYLYVIIRQHMLSEGSHKIVVQFPSAHLAKLYAHLFQALGFPAATAHSRMGQAARQEAVRDFAAGHRGLLFCTELAGLDAGCGLTMVLWVGLPHRPSQLVQHMATIQQAASVAAASTAAGAATGAAPGGLLGPMDAQGLHLQTAPVPTNACIPTAGAVPSTTGSASEMIATAVPGAQFPAVTAAHLPLQPARCLLLLSDVEKQRPVILAAAEAMAAAKLGRVLPVQPGGLSGPLDSVSRRIQQVLPKVSPSLKLRGLEGLLGHLWSSRSGAAASLSSCVAGAGLGPQPEEQLGIWARRFAAAMGMPQPPLLYRHVAARMGLRSIPGITLVDVHAGRPPLTGSQQLSAPHHRGSEHYYHQRSISWQQRRLQIQRQRQRQQQIQQTLRKAQKREQMQERQQKQDEERQRRKERQGQQQPRIERPKLHPPEVIGRFRWFRALKRTQKAQAQAQSHAEPPADVQERFREGERRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SII5</accession>
    <name>A0ABQ5SII5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Multicopper ferroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013933</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF486">
        <property type="entry name" value="MULTICOPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="341"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="534"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1160" mass="125164" checksum="B21A8C9F5D29C84D" modified="2025-10-08" version="1">MISAPAKEKQPEMSMTVDSEPNSVFGSGTDDVKPGQTDVESPADVASTGKATPFPAAAATADADGGKRRRVLWIALAAFVVVAVGVGVGVGVGIGLKKKESNIESVLSSVELGGVPGRTYFIAADQVSWNYAPSGRNLCYNEEVAAKFLPTGTTRVGGTFVKALYREYTDSSFKTLKPRTTEWEHLGSTGPLIRASVGDVVRIVFKNNLPADISVNMAPHGGVVAWEGAGRKSAGTAPVAPGATTTYLWLVPPEAGPAPNATVSSRLWLYRSSVDPTTHDNAGLVGPIIVTAADGAGPDGLPNDVDRELVVIFQVVNERSSNMLPYLDSHLTGGVSFTKMAMNGFVWCNLPQSAISLNVGQRVRWHVASVGSSDGLHNYHWHGHTVELNGHHVDQFTAIPGATYSVNMIPDEPGTWMFHCHVNYHMDGGMVNLYTVQGKAAPLLSTGVERTFYVAAEEVEWSYTGPNNTNMCGDVPVAFSDNVTGHQFVDGPWAKGTDSRLGYKLTKTLYREYTDATFTTLKERGPEDAYLGLVGPMLRVVVGDTLKVVLLNRAAINVSMHPHGVRYSKASEGTLYEDGTTAAQKLDDVVQPGQTYTYIWQATERSGPGPRDPSSMLWMYHSHLDETAETYAGLSGAIIVTDPVRAVNATTDPRPIDVDRELVVLFTVTNELESSNFMENLRSRLGDGGALAAHEDAVESLIADAGFQEHMLKHGINGFMYCNMPRLNFTQGQKVRMHVLVLGTLEDMHTPNMGGPRFDYNSQHTDSIQISPGGMISADVVMNSPGEYELQCRVSDHVTSGMRAKYEVFPDLRVVEATTPTSVVRKYYIQAEPVDWDYAPGGYQCNGVVDAGVQSGSNTYLLKTSYTMGSKYRKAVFRAYTDESFTVPASMPSYYGTMGPTLVVEVGDILEIHLRNNLTDLSYPVNIQLGGGLVPRGQNCKEVAVGQEACVYRWFVPSSAGPASDDFDTTVYGYTSTLDVAGAPNAGLAGALVVAAAGVLQPSKLQPGQLVPAGVDLMVPLYWQVMNEEQSPYWDINVAASGIDVAQIEKTSLSDRFHEGNLKHAINGYLYCNLPGLNIPYGSTVRWLLVAYGTEGDFHSPMFGGQVTKVDIRGFSTLASLMPSIARAADMRAVVKDTWLISCDVHDHYAAGMLAKFTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIP6</accession>
    <name>A0ABQ5SIP6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxyvinyl-carboxyphosphonate phosphorylmutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905:SF5">
        <property type="entry name" value="CARBOXYVINYL-CARBOXYPHOSPHONATE PHOSPHORYLMUTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13714">
        <property type="entry name" value="PEP_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="36653" checksum="DED3E9A18EDC3A0E" modified="2025-10-08" version="1">MKLSSNVTASRAPLGGRSLRRGVVCGATNGSNGARTTFHRLIEENGCVLLPGVYDALSAKIAARAGHKGCFVSGYAVSATVLGEPDVGLLTSPEMARKAGQIHGAVPSLPVIADADTGGGNVLNVQRTIRQLITSGCKGCFLEDQAWPRRMGHMRSKEVIEMEEFAAKIAAAREAIGDSDFFLVARTDARGISAKYGLEEAIKRANLYSDAGADATFIEGPRGEDELRIVGKETRGLRVCNMLEGGTTPLHTPTELAEMGFHLVVYPLAALYAATRTLLTAYETLAAKGTTRDDMAGLAHFDEFNEIIGLEERIRTEERLTKREGSDKLHVRVRAPVKELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIY8</accession>
    <name>A0ABQ5SIY8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="59816" checksum="82345649D47FF6B5" modified="2025-10-08" version="1">MEQALRFLNAMVNAKGSEGLLPRSTGGTGGGGSWGNAGGGAASSAGGTTTGGSGGGGGSRNKIITVPAHVLAPAKGKGGKAGGGGGSPPTPPAGSGVSTEAAAKADVVCREGQWQAHQHKTCRLHVLARTPHTQAACKVSASRISTDPQGLFRDMCRKLQEAQQAGQVPRDLSGSVVQAQQFLARAAAPSMTVTIEQAQLQRIEDTLQSVLAMQQLPSSAVSEVAAPVQPAALAWRPPAPPVPLAAPSIAPSHVTATGVHAGPPAAVYRPTHTPVQSPLMPPPPPHTHPPWQPGTWPLNAVAPTGSSAPGDAAVMPAPAITMPFAGYSVFPIGATVGERMPMGFPQLPRAAVAGAAEVAHALRPPTPPGAGDATAVATIPDPRSELRPDNSRLDDIQGWLLAAPAGLHSFRRRGLPSAALPPPYASPVAAAVSHSSAAAPLCVAAPLPYLQQNSAAEGLLVEGQLPSQVLVDTGSAVVLVAQRLADRLELQLDPGGEFVVTTAAGRSHLKGLVAGGLHVVFRAGTPDAFAANVPALVVADAGAEAFELLLGMPALGPHQCYWIWRKDVSICGCGHRAATPSTSRSSYGGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJE3</accession>
    <name>A0ABQ5SJE3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">THIF-type NAD/FAD binding fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014886</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004281">
        <property type="entry name" value="PRK05690.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF102">
        <property type="entry name" value="ADENYLYLTRANSFERASE AND SULFURTRANSFERASE MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="2">
        <location>
            <begin position="103"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="465" mass="49191" checksum="2EF680C31B58F6B0" modified="2025-10-08" version="1" fragment="single">RSRVVGSGIGADDVGSSEEDRASGITTTDATLTRGAPTTTAAPSMQFPPCLATSPSSCTPPSSCSWQQQQQEQGPGDHEGTLRPQPPRAAPRHYLSRAQVERYSRHLLLPGFGVAAQERLCGGSVLVVGCGGLGSPVAMYLAAAGVGRLGLVDHDVVDVTNLHRQVMHTTSRVGLNKAASGRITCAAINPAIQVEAQTRGFTPANALELVERYDVVVDATDNPATRYLVSDACVVSNKPLVSAAAVGTDGQLTVYNYGSDGPCYRCLFPEAPAPENCSRCGEAGVLGVVPGIMGCLQALEAIKILSGVGDVSSKKLTIFDALAGRFTTVRLRNRNPTCMACGSSPVITRITLPSYDYVLFTGQPPDDGPPADLELIDPRFRLKPPQVARLLAAQMEMDVEATAWQRREAVMAEPEAAIGKAGPGLVDSFSPWEREVEQQGRALQGDQQGQQEEMHDTVNEIVDSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK75</accession>
    <name>A0ABQ5SK75_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="129" mass="14196" checksum="2C3BC9E97990C524" modified="2025-10-08" version="1">MGLPPHTPPVAESLEQPVVTAMGFESISPDGNVSALPIKLCCLLITNTYLVACMGFFWFVGLVSIYVGGAGMNNPRLENLLALSFWLLGHVLLYIGPWKRETLALCVQMLVLLYCVLPMSWFSCLINSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKG2</accession>
    <name>A0ABQ5SKG2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014904</name>
        <name evidence="3" type="ORF">VaNZ11_014907</name>
        <name evidence="4" type="ORF">VaNZ11_014910</name>
        <name evidence="5" type="ORF">VaNZ11_014913</name>
        <name evidence="6" type="ORF">VaNZ11_014920</name>
    </gene>
    <organism evidence="2 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="2 7" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="232" mass="23970" checksum="72B23A3BFB0AB7E2" modified="2025-10-08" version="1">MQIPMKMIPLEAFCYDAYGLYDHRPWTPAETEDVGLAVASTARGCNEPGPIKTVKTSASMEAVHDPCTSTQLADVQHRNGPLSRKRRFEEMAAEQQGLAGREGVVAAEVAVATEVAVVAVDGAAGAVGDAAAVAAGVETGVEAGLDAEAEEGQGEERATRQRVANAPDGKPVAVFRGQRPRPRAASVMKVWACGIMAWAVPVVAAAAVATGGFGLGEAVCEVLLAEGMAGWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKK5</accession>
    <name>A0ABQ5SKK5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014732</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="146"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="427" mass="46336" checksum="D0B9B00B8081B3AC" modified="2025-10-08" version="1">MGCGSSVPVEAADQQDQQQKHSQQSSQGQTSVKQGAGNADKSPEESTKAKTTRARRLSYISNDANGAAVNAPPGAAAPATSGPGGRSGNTRARRLSYVTNDPNATPANLKFSGEADGNEEGELEGADEPTVQGQLAPEEQERRTLRVNVACMSRAGREPGFKKTNQDNCFAFEKYITEDQSLFGAMDGHGPHGHLVSGYVKQHLPIILVNHLTLEKDVKKALTQGFCEVDRSLANSRIDCEFSGSTAVVSYLKGKTLTTAWVGDSRGVLGREGKRGWEAIDLTTDHKPTAPEEKARILKSNGRVERLVDEMGQPMGPYRVWLQYAWIPGLAMSRALGDVLAHQVGVTSEPDHTVMELTPQDKFIVLASDGVWEFISSKEAVEIVAQYDSAEEACRQLVDEAYQRWLTEEEGVVDDITAVVVRFIHPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKT3</accession>
    <name>A0ABQ5SKT3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015532</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1143"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="775"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="550"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="780"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="800"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="829"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="868"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1228" mass="126522" checksum="DAF2242DC394DF47" modified="2025-10-08" version="1">MVSDSLGPQRTLRLENAAAAAAGVIGPQRCSEAAGAGSGCDGHLSPPSTLFISNRNCLGITLMLPLPPPAVAVHGQAAPHDKTGPTPNPQPSSPTSSSNQSLAPQSHPRASIPSGLQPCCSSPPARRETAAQGPSQHRQRHQQQPDDAGKIDLEPTPRRQPQQPPLPPPPPPPPPSQQPFPSGGQIHDLICLTGQESSSKNPSPAAGDRSADAEGNPYYFRDGSRRRSRRRRSRRRRSSSSSSSSSRCRVRHQHLRKAAAASHSACGGITGASTGSLSRGRHHRYGFNTLARLRPLLHYAAAGMLMLAACAAAYGPPFYLSPSDPLSMCPTLQTDAYLNSTCKLKYSDSTAPCNVMVTCPDCLGGSTLPTWTVFVLPDRSRTKANATQILCPNFNLNDDNIAELILIFKLGDSCPPDNSAIDCRYVPSPPPPPTPSPPRSPSPPPRPPPIPSPPPSPPVPSPPPLPPAPPVPPSPPLPSPPSPNPPWPPAPGNPPFPPRPSSPSPPSPLPPPPPLPPLPAASPPRAASLPPLPPVPSPPSPPFPPLTPSTASNTQLEQAVQQPAEPAPEPPSPPSPPPSPPSPPSPPSPPTPPSPPTPPSPLLLPPPPVPLPPPSPPPLSPPPSPPSPLPAPPPPPSLQASLTPLSLPPLWPQDPSVPVLPPPHPSPRRPPSPRPPSPPPRPPPPRPPPPPAVLVSVPPRYQVSSYFGITSLPTNARLKAVSNNIATQLGLDSGAVTTTVASAFVATVYSVYTSSGLTSGRHHRSRRHLLQTSSTALNLPSPPPQLPLPVPSSTPPPAVSPTNSTLSPSSLTNSSGSPPPLTASSTATPAPPPSASAPPPPLPPPTSTSRPPPPLQASLTLAAITIPAFPPPQPSPQPSPPPSPPPSPPSPPPPPSPPPSPSPPPPVPRPPPPPRPRPPPRPPPPLAPALLFPSPPSPEPSVPLAPSPPPLPPSPPGCVTCSQLLLQIMLPNICSQLDVPDCSTIRLSCVDPTANVTSSFPLLPSTATGYGDNCSSRLTTVFYINDMSQQASLALKLLTTQVAVPGFQIVSPDSSSELPVSSTLLVNTVNQGATALTVSDQAVAKSIASTLGVTGGQVFFITGPEGFTNGSSSSVLTAVASPPPPPSKDCEKPKLGSLCGAEAVGAIVGIAAGGLLLVGLLVALAVCLINRGRQPVVPVDDYAWVQKYAPVTPGLATGGYSPPLFRASPYSMDLNQPAYYYGTAVIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKZ4</accession>
    <name>A0ABQ5SKZ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polycystin cation channel PKD1/PKD2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_015604</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013122">
        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002153">
        <property type="entry name" value="TRPC_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117:SF54">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL-GAMMA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08016">
        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="855"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="893"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="929"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="970"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1031"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1155"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1315"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1347"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin cation channel PKD1/PKD2" evidence="11">
        <location>
            <begin position="928"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="178"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="435"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1204"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1522"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1605"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1669"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1803"/>
            <end position="1865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1944"/>
            <end position="1975"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1566"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="440"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1217"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1525"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1605"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1617"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1706"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1750"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1851"/>
            <end position="1862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1950"/>
            <end position="1975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI70665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1975" mass="213455" checksum="53958B1F6578FBB9" modified="2025-10-08" version="1">MSHGAVGARGIPCSSNEEVYPSNNWPTRSARFRAHPSSEDSICNYAQENAGIHAWWTSEGPFSGSILEPGPQGIAESTEKRVTAFTQVPSNVARELSAAAPSMTSMTNPPELLTLPASLPISTAPSVSGHIHKPTPNRVQFFDVLGRVPGEVPATPTTRVVSDLNETFGRSFGFSTIQAPSSPERPSRFATGTATTRRLQSSRQRALSTTSRQGPMCHLGPLERPQEVAKLRLEITLQHSTTIEKVSAAELTSFAVGETPFVNGNETYRATVRAIRGTSELRRGAFYVPSEYTDAAASGSREVMSGVLRHCHRGTQVLVQLFVLFPSLMRPPRWLRKDFTLVKRANVEIDWQLPRRTQFLLYCLRRLDHAQNQSLSVWASKRRFGVGEQFTLYGAEGRAEPTDWPYVLVLKFYERREGPPPKSMINMSREGPLRQAAISGSGGATPSGGGGTSGDISLSPGHMSGAARAYDTLSGVTSAMSFGGVKRGNVQLDALHEVQQQLLLRAQSSDISGPIPIPESGLITWDSVLEYTLAARDGVSLRRLIDSHYLDHLLKSQDTVDRVEKVALSNNDPSLMMMLYDHTTIEMDLRVLAFLVHCWAIQPLTGRAANANANLTAFDAGYDDFPDIFDGADDLGGTLAARIEEFLVKYVLQRRNPLSATAAILRAIDDEMALHEENRRIMRLASTRFHSFHLAMLKRLNRYADVRNIRLLERILQPADMPNTVNEVSPLQVAFDSRDLKFMTESMIELYVKVQWLGRELLALTRHEDPRALGATNPLLAYAVLKSMGFAGKGTMDPMVMAASRLWHLYTFTSTAFFESPRGRWAMHVLFELAFLGLYQWQVMYPNWDTGSLRTLHLVFLTFIMGNLLDMAQFVHYRYGSLARIRRSMADPWNALGLVVNGGLMVLTCLKLAQDFGAPGIPPYKSADTIHVCMATLAVFVWVRALGMAVPVYPSLGPLLNTVARMMEEVIAFLFPMLIVMTGFSTMMAAIYQDLASEYNSFTQAMLQLFSSMLGNFDFTAFDNLTTVQHLYGIIILVIFLIISAILLMNLLIAIITNRYRPQEVEAESQFKKAQIVSYYQTQVTRNLVCSPFCLPQLMLKTVPLLPSGRRNKLSGAARSVAVLGLVPLDGVVLPDDGSDRPTGAAEIPHLVFLLTLYPIMAFTCIMLFSLYTPFAVIQFASKMYIRLVKQYVKRMEERHRRARLKANIIHVKSHRRDAPERSKKGKMEKQPKLGSPSPSQPGGATDVESGIAAAAAAPAATATAAATVNAAGAAGASVDRSSSTKGDSSKQYQRLKKELQADGHAADMGLQHTLGYLVVSSVLTVVGLVFYVLVLLVMMLVGWASLYLWVAKLAFSLYNTLLRPFVDLVRARQRQRRLSPVTVAASALDVANGVRTKAATSRSAPGALSMTGCGSGASAGGSAISTLLGGSGGVGGGHGSGGGDGSAAGCDESDQLYYKQSKARIHWRHAILKVIAMKNERYLTRRDVLKALAASKFRPHMVGWNLRVDLRTSSQDIMESQMQDLEDDNDPWDDDEPQQGGDDDTMEALILEKTENLTSMLKNAVNLQQGAMATLMERLQQLTEEVAALRADGAGAAIAAVGDADDGVSDDDDIDGGGRHGDVARWRPPPPPTRPPSEWGLAAETGLKSEEPSGADSNHLIGLGVRLVPELPQPSPGSAGEHSLKGKKLSPEAEGEGDDDGCGLTAATTTNDDAGADSASASTALAAAAAAAPAEASPPPSPPGAARVPRLRPKTAPPRRAKLTAVAANVVPAESMATATLAPPPPLKAFDEDKMTAPIQQHRTTARPTHGTPARSSLPPRHPKLPATAGLAATPSNVLHAPGETPSQISSTHGSVRRSSSAGDGLRSLQHQMTLERLLNSSGGPDVLLAAIGTVHERHDGGGAGGEGITPAAKPTYPTILEPIRNMSPAPAPAPTPAAAVVTVVTPPLLQPLPPQPQPQPQPQPQPQPQPEPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLM6</accession>
    <name>A0ABQ5SLM6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_015635</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methylthiotransferase family. MiaB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01864">
        <property type="entry name" value="tRNA_metthiotr_MiaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006463">
        <property type="entry name" value="MiaB_methiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01574">
        <property type="entry name" value="miaB-methiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43020">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43020:SF2">
        <property type="entry name" value="MITOCHONDRIAL TRNA METHYLTHIOTRANSFERASE CDK5RAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00273">
        <property type="entry name" value="(dimethylallyl)adenosine_tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="9">
        <location>
            <begin position="97"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="234"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="8">
        <location>
            <begin position="476"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI70694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="571" mass="63421" checksum="43AF38523C2C6636" modified="2025-10-08" version="1">MMFRSVLHHPQPCGSVPQNVLLRAARGPPPFKPCGNPIAIRHRLQMHPLTAIPGVDVGGLTASAILDFDESEASTYCGSEGQVLVRKAPENIAKNGKKYFIHTFGCQMNMADSERMAGVLEAVGYECSQDAGEADVLIYNTCSIREKAEQKVYSALGKQAKRKRNHVGDLKIVVAGCVAQQEGQTLLRRVPELDIVMGPQFANRIHDLLDQANDAQVCATEQVAVEEDITTPRRESNITAWVNVIYGCNEKCTYCVVPYTRGAEQSRWPDDIRREMLALGEAGYKEVTLLGQNIDAYGRDLPGLAADASGRRAWTFTDLLRHVHDVPGIERIRFATSHPRYFTDRLVRACADMPKLCEFFHIPFQSGDNDILRAMKRGYTHERYRAIIDNIRRYMPDASISGDAIVGFPGETEEQYLATEQLVREVGFDRVNTAAYSPRPNTPAAEWDNQVADLIKQDRLNRLNKVVMDVATERAQRFRGRTLEVLVEGPNPKNPRQAFGRIRHNKLAYFDGDGNELRGKLVMTRIEECNAFSLFGSLVEVVGSRAAAPGTLPLQPPPLLAAVPQLPLAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLZ2</accession>
    <name>A0ABQ5SLZ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015586</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="97"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="288" mass="32724" checksum="25D33627D36598FC" modified="2025-10-08" version="1">MPLHSATPLHARVTARLAAWKAIGADTRTLHVLKHGVPVEFLGDRPPPAFDLPSFPVTIEQRRWWLEQEEPRLIALGAISRVEAPQAVKHVVNAFCVPKPGSGSWRLVVNLKRMNIAQKAYKCRYESLCTLRRMGIQGSWMVKVDLADAFYHIPIREADRRFFVFRFCGVLYQVNALPMGWLNSPYWFSKMIRNVVRFWRDPLASVGGGRPHLKPPLPPHQFYPPAWCGRPARLGVRCFRTLMISCLILLPSSKPVLEPSGSRRALNSWAYLAIPRSASGRRLSPFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM01</accession>
    <name>A0ABQ5SM01_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014803</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000091">
        <property type="protein sequence ID" value="GLI70176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF31">
        <property type="entry name" value="OS01G0873500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="222"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="470" mass="50670" checksum="74496C2B5737DE58" modified="2025-10-08" version="1" fragment="single">MGKDKLAEPLDLNNYSTWAYRFENLCAEHNLNRALLTEPVTDEEVAASERVKTLLVKNVKDCHLPVIKASPNAKMAWDGLAAIFAANKHARRSQLRQEYTKLAMKKGEALPVYLARVRELHTDLLKVGQATSEQDMTYAMLTGLPQKYEVIVSILTTQSGELTLSSATAQLLAFEARVGSMHEASTSSSAFVSAGRGSSATGSAPRRGGSHVRGGSREPLQCWNCGKPGHLERGCTAPRMAQRSGSWSSAPRGPGYGSGGLERTRMGGHGEAAGGPSLGSDRRGYGRHDHVDGAGDRGNASPRNFAFVASGSDRLKGWYLDSGASHHLTYDADQIVDVRELAESEKFEILSVNGGIIRPTAVGTLRLIPGVNMHARVTVEEVYLVPDSFANVLSVAALDKQGGSVCFEDGQVTMRKGGEKILTGQRLGDLYALSCFSIPRSASRLRYTQPFGGVAHGKSASESMDLWHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM32</accession>
    <name>A0ABQ5SM32_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">R-SNARE, VAMP71-family</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_015974</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the targeting and/or fusion of transport vesicles to their target membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="12">
        <location>
            <begin position="7"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="13">
        <location>
            <begin position="126"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI71058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25512" checksum="295C21D0C9F64182" modified="2025-10-08" version="1">MPLIYAFVARETTVLAEYTPYSGNFNTVAIECLQKLTNPESKFTIACDRHTFNFLVHNGYTFCVVADEAYGRQIPFAFLERVRDEFEARYADKARTAAAHSMDRTFGPRLKNHMEYCMEHPEEISKIAAVQKKVNEVKDVMVENIEKVLERGEKIELLVDKTDDLRNQAEQFQRKGRQLRSKLWWQNCRMKLLVLLAVLVLAVVIFLLACFAGGNNCVKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMJ1</accession>
    <name>A0ABQ5SMJ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="43980" checksum="189E61BE65E459EA" modified="2025-10-08" version="1">MILAEGSWPESSWGQAHKLVKIPSPWPSPPSERRRQPGGETMESLIASPPSPRSDDHNGWTLVERPLAEGVTEAIDIRPRAASPIPELAAEALSEREELQDGMKSPESERLADDVLREPFQAGGPLLLQVAAPPPAPTVEEPATPTAASGPHSGSCGDLSCSHFHANDLEHVTRFSDHTNLASGGVGAGCVSAAGWGFDRHHHERLRAVNRLSGEGALEGFQDPQDLLFADSAAPGAAARIGCGVATDVSADDESGGVINGPFGTVSLGWEALITNVWSDLLRELEDAKALVTMTAAALWERVQEGRMAVEDWATAVRESARKSAKSMGRPGCPLWTLIGISGLAAVALAALATQMVLNRRLTSQLRQRDKDLARLVVKILNLQDALQSASRAVPVLRQASLGRFTTTTLIGMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SML8</accession>
    <name>A0ABQ5SML8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CreA protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010292">
        <property type="entry name" value="Uncharacterised_CreA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37952">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37952:SF2">
        <property type="entry name" value="PROTEIN CREA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05981">
        <property type="entry name" value="CreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046892193" description="CreA protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="22292" checksum="86F78738DC66F260" modified="2025-10-08" version="1" fragment="single">RNVHGGNTCASIAAFLAGASLLLDAGLAHAGGRGQVSEFTASGLLFKDVVEVTALEDPEIQGVTIYFSDFKRNIVDKLSKDFFSDPSQASLTCALTGPVAIKDVKRISSFEGEEVFSEQKGLNIFKNKTLRVRRVYDPERRTVIYVAYSTRLSTATDEGGVSTGRYRTSMCAVTLPPPASAPSLVPVTSGSPNATTTTATAAEVLFSDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMM8</accession>
    <name>A0ABQ5SMM8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">18S rRNA (Guanine-N(7))-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_015594</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. BUD23/WBSCR22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039769">
        <property type="entry name" value="Bud23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022238">
        <property type="entry name" value="Bud23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734:SF0">
        <property type="entry name" value="18S RRNA (GUANINE-N(7))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12589">
        <property type="entry name" value="WBS_methylT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="9">
        <location>
            <begin position="58"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23 C-terminal" evidence="10">
        <location>
            <begin position="204"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI70657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="31463" checksum="A1F1DED6C15A386F" modified="2025-10-08" version="1">MGKGERPEHMAPPDIFYNEDEARKYTTNSRMIAIQAALTERALELLALPHDGVPRLLLDLGCGSGLSGEALSEAGHVWMGLDISAAMLDVAAEREVEGDLVLNDLGHGLPLRPGSFDGAISISAVQWLCNADRRGHDPRKRMKRFFETLYACLRRGARAVLQIYPENHQQAEMLVASAMKVGFSGGLVVDYPHSTRAKKYFLVLMVGTSTAVPQAKGLDGSEPEEEEEAQIKMAGRDRFKRRKTGSGMKGRDWVLRKKEQMRKKGYDIAPDTKYTGRKRKHIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPR3</accession>
    <name>A0ABQ5SPR3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thiamine thiazole synthase, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Thiazole biosynthetic enzyme</fullName>
            <ecNumber evidence="6">2.4.2.60</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">THI1</name>
        <name evidence="7" type="ORF">VaNZ11_016179</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:55708"/>
            <dbReference type="Rhea" id="RHEA-COMP:14264"/>
            <dbReference type="Rhea" id="RHEA-COMP:14265"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:90873"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homooctamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">During the catalytic reaction, a sulfide is transferred from Cys-265 to a reaction intermediate, generating a dehydroalanine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.60" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000102">
        <property type="protein sequence ID" value="GLI71106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03158">
        <property type="entry name" value="THI4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027495">
        <property type="entry name" value="Sti35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00292">
        <property type="entry name" value="sulfide-dependent adenosine diphosphate thiazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422:SF3">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="156"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="346"/>
            <end position="348"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="2,3-didehydroalanine (Cys)" evidence="6">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="392" mass="40739" checksum="9D77ECF211057916" modified="2025-10-08" version="1">MEGGTIRCTLPPSLPTGSFLASELLHCYPSEPKMATMIAQRRAVAPTTAAAPKRGARLVARVAQPLTQPGSSGAQAADASMLARAGLPPTTTPYDDYKFAPIREAEVNRAMTRRYFKDMDEFAESDVVIVGAGSAGLACAYELGKVAPHLKVALMEQSVAPGGGAWLGGQLFSAMVVRKPAHLMLDELEVPYEDEGHYVVVRHAALLTSTLMSHVLKNPNVKLFNATAVEDLIIKPDVQLGGARRVAGVVTNWSLVAQAHGTQSCMDPNVIEAGVVVSACGHDGPFGATSVKRLARLGMVPGGEVPGMGALDMEVAEGAIVGGTREVVPGMVLCGMELAEVDGSPRMGPTFGAMIVSGRRAAHVALAVLEKRRRVSAAAAAAANAAGEMVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPY2</accession>
    <name>A0ABQ5SPY2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Globin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_016851</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00454">
        <property type="entry name" value="TrHb1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF5">
        <property type="entry name" value="GROUP 1 TRUNCATED HEMOGLOBIN GLBN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI71585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="46645" checksum="2B66D024138604AE" modified="2025-10-08" version="1">MGNTCIRAADGITHLVTETEIREAVRGIEEWQKMQASTVKSGAAGQNTYGPLLQRVGGLDVVKKVVELFYRKLYADPRLILFLHDQDVMHLRAKQSAFISWLFGPPDVPYLGRSVRIAHLRIVKQRGFIGDDFDLGMKYFEQAMAELGAPEAIIGEVMRRLRPFKDAFFTPSPKDAEEEARWVTEEREKEEAARQHQLEEQEEEAYHARERRLQEQITLAHQVAEEAEAAEEEAKEGGHNAAVAAAKMEELSAGVIRPSSRGSQCPFTGGSRFAVASASGAVPAASTSTPARPATPAATAAASANPSSGCSNGAASLPLASPGQTVAASVAVSVPVVSIETSAADPLAGPSLPAAQLQSRPPEATADRPGSITITDQSALTLAAALAAVVTAPTIATAVPDPAVDLAAELEQIGRRSLFDGGKASSERVPLMPPSQEPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ58</accession>
    <name>A0ABQ5SQ58_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="50256" checksum="16174F50D324917F" modified="2025-10-08" version="1">MCKGWVSWMIIWVIGISFSRLAYVTSSQDTVAGQVKQRKLGSTDSSLTEKSLHGLYQRPRRQLGESNPGLAASYMDELYRDYSTARKELVGDFKKCNRIAVVYMCFNRAEVIRRSLPAVMRSEGVEKFDLFISQDGGADYIINQDLHFPESSSIAYIRHKANLCTGLHHHFVKTFTFDIMGYDGLIIIEEDNVVHPQALQLLAQMIDLSIDQREIGIVSLLDMDNSAFLDDRKYAEGVIRVNGTMGHLWVYGFHKSRYEAVRSDLYDYFNTIKGQDYGSKHEPPLSDKIKALLVSKGFPADGELSQDRFFIYSLEKSGYRLRYQTMFRFFEPIGYLGLHHRHTEEEFFAMFGRGMFNGRIRPQAAFDVTKDVTVVFDIKAGVRDHINRVWLKYFPNEVHDKLVVNTFNSLMEGRLNGVEAMADIKNAGIKYLASKSGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLN3</accession>
    <name>A0ABQ5RLN3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="28702" checksum="69E8153CE0DAC66C" modified="2025-10-08" version="1">MPESPRATTSTKMSNSIDRPPMQLNHIELCRRPLRRTVPKPQGDKTGKSRLRGRGVPPPNGKEWEYHPSKQFIPGKSFASSLGSIMRQQGDSVTDQGTPRTSVRMSDRSHESHLSGGGVEVVSPVKPFKPLRRMGSPGPETARVLGIKANDTRDAALPTYALLGTARRDVFVTSRPTTAPACVLLTKTGLRSGYEKGRSAYDDVVQYRHSGGICPPEYKMLAERPAAPQLGPSSNTQARSSFLDSRSSYQINKMRGQMSDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMI7</accession>
    <name>A0ABQ5RMI7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carotenoid oxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000407</name>
        <name evidence="7" type="ORF">VaNZ11_000409</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="6 8" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF138">
        <property type="entry name" value="CAROTENOID OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="193"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI58664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI58666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="70281" checksum="C09B0D6C06075DDD" modified="2025-10-08" version="1">MIQKRVRRTTPITVATLNDVRTMFTSLLHEYAYWVDESWVTGTIPPELYGTYYRNGPALHVANPRYRRHTMDGDGMVFSLAFKDGKAYFRNRFVRTKGFLKEQAEGRPLYRNTFSRGSHDSNNPWFNPFNLSFKNVANTNVLPWAGQLYALWEGGLPYLMDPTTLDTYRESRMGGQIRSNMLGAHYRIITEAPDETHPPAKTAMAPRRHQSQHAEQKPHYSHQPQQKWPGASGGASPSAATATCVGADGSQRLVAFSNEYGYGGAFVVFYEFDESGKLLHETKEKLLGVDIALIHDMVVTEHYYVLVLGSIHLDPFKFVSQYALGLCSIAEVLVFDPKLPTRIMMFPRPGRPSRKALTPHVLTTDPLFVFHHVNGWEEETADGGSVVVLDCVAWDKVSFDMGFFDKHGQRVDNFVGGSRMQLTRLTCDMYTGAVERRKLLQRTVEFPVTDQRITGRPHGVFWCIADAVDHPVFWGPPQGVLRVEVDPGHDLTDVQIRSTAARSRLAPPRRAAAAARGEAACAASGVAVDSWYFDERTFPGEPMFVPRPGSTREGDGWLLLATHSAKTERADLHIFDAEALSAGPLATLHLPHHLPVSLHGAWDTTYRGPDPHDSSVPRWQDLGSVKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMJ0</accession>
    <name>A0ABQ5RMJ0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1067"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1237"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1067"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1244"/>
            <end position="1257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1455" mass="152395" checksum="C8B8E52F33845F39" modified="2025-10-08" version="1">MAHDVARVVQATESGKEAADTLVVPEAPKWSKLIICGDDKEPYKFRWSDAGRHGVLVLRRWVFLQHLSKAGHGQGASPSSVSPYSGPARCVSLVQDGPDCGYLARAAAAFQEAALTELQRVALRELQDCAAVMEPLDQPGVVGDGSMTLAVLHMWRRCPMWLSLQRAPADLMGDLNDALVGELAQGSRAALHNVMAALIDGWRLGLAQLGDQLARRVEQGADHVLGQREDVSVAGGPGSEKRAENGIVKWLLWWRLYLWLHRQMEVWAKSQRGAPLLDPHLCSDSNADIDAKQEGRLSARHVDRWPAHGKSCHHPPVPPSSLAAACVDGASGGKTHGHAGRHHPAVYAGDEAMPSGAVPQPPPALLASFLSAYARRVAKATLELSDTAVGAPRNPLAPRCPSRTSANGSLSGGRQQLRWACLQMLRQLGEVLADDLSEEDVVCRIQQLTVTRGDSNLTGSHKVAPAFSAASALLSRPDRATLSASDRGDSWLNAPSRPTSLLSSEPSRKSALDAVALAPVPSRNGPVVGAHGGAAELPDTAAVLPVDVLSELRPERILRLSNSPGLLSLQLQLTQSLRSSVGEVLPGPNRPSAATEDKSARHARRAALLQGVCWSLHQIHRQLEKVYCVCCLSGLTAEEMASLCDREFQGLVGSRCAPVLSPCCDLQRLPVEPVSSRVEAVSTAVGAAALAAVSARPLPTADSTWALRAAAFTHADAWVAARPTKKCSGSRSGDCHRGADSGGAAAPACVGTASVTTVKGRITEASGINATWACEVERLTSALLAGGLHRKCGSRDLVAEATSACASAAIAEGGVNHASGDGGVYSQCDHASAGLIRGALLLYAGLVRLLGTLLGAAPTINPRGMPLYRWAKPDPTDVVAEAAARASVADCAADTPATVTDASYTSSASAALTQPITSGYLHNGALELHQGHHLGSSTARSLLFHMAVSACTVMANRLVHVTKTHAPGSGANLRPLCVAHSDTATLWSAAHALLALVTFAAVGSSQVGNPPPRFWRCRRTPFGGASQSLPRGETVPREVGEHSSSMSGAVMDPHTFPSCSMESALAAAGTGAQGAQGSARRPSRKLNWRRSLRRIMPYRTERSSSHDSSQSLLRDRSASGQGADLGQPIAGTQRKSVRRACWAGEPIEPVPLPAPAPLCSIAAPHLEAAEAAHARAKTAPLLRRWPFSETTPAAVHGPNVAVGGGSMCSGLADTRNHWKKPDGSSGGSGNTCIVGMGSSHTKLGGSKRSQGGSGMRQQQRLAGQGLLVLSDAELGLMQVLHEACGQLRAATMRAGDLVAAEMGQRAAVICRSSAGSYDSQVLPSAAQQVIASLLKPLREALEGLHPVSQSELLTRGFSAVVQALRQHYLGGIRGGGRDKRNESGLAGQELGKGLTLLEAELAAGWRQLGAAFGCDGCQVDTPVAAWVAGDYEFAKARTEMDALIQSLRNDIGTMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN25</accession>
    <name>A0ABQ5RN25_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="609"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="696"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="885"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="925"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="963"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="474"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="644"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="703"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="925"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="935"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="996"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1054"/>
            <end position="1066"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1158" mass="117906" checksum="9B96E58AE246B2B9" modified="2025-10-08" version="1">MVNNVSEDDPGHGLACGARSFDCFSYVDPTNYERVGVKLVLQACICPAAVLAANVSLLDGEDEPTHAHGIPCADTFAIGPRGLEEYRRMCRSQLACGFMCMGLPVIYTENLIQRQRIRSKYGLRDPTSLSEIGAGCCYPCSIFQSHTFLYVSTHRPSKPRPGVHASHGPLSGALRAMHRATSTSKQRSQDVASTVRQETHTAGASASCPKPIEPPRVSLHHSCTTPEKRPRQEATATAATAASGIQEAPAASAGGCTASTSLTAAAAASAAAQRVPSASCIASGFGETPDLMVTSPRHEAQTNTRRAAHRIPAASPASAESRSTPASSSIPPPEVTDSPPVGAAMIASTPEVEDDDGDEVSSALTDSASMPIPSALPVCTMALSAAKFEFSPPQRPQTSVNLMAIEGPKQSQHMSQTRQRHEACRLQALEPAISAGQPPVKPPAMETVASGMSASDVSLFSLALEPGMPSLPQPASTGAPTPSSGATTASSAAVRAGPGPAPTPLPAPPLPTPGSSRDLHPAAYDTITSSKLSSMIDHAMLNGSGSATGRGSGSAAGGGGTALSSIDPAGPWDIYRHSVMAPIAEGDAEEGQRSIGKVAVHLPVSQDEAYATPDSDAPGSVASASAQRMSPTKSRTFPRHVKVPGSSAEPSPPGSSSLRSARGGRRGSGGAFGTNPFTDPVCAGGAALTALARMGPEGGSQGCGAASSGAGRLGNGLSSRKLRLHGAPPAPRPSPPPRAATLQLERAASTDGVRSSYQRVTYPDGADEDDDNLEDMSASADNFSLTNALPSQLLLNGAVGQLPQRRGSSQGRYGGVAPERPVMAIAAAGVAPLPAKQRHVATGLVVRDDDDDEVNFASIRPSFIDGPEMANLKLVLRAAGEQSADGSEAVSVAPTGPPPPAGHVMMPSSGSQRVVALSTTVTALQQRGSGSRRYSNVPPAVPSSSSTALGRGAVTDAAPYASYCGDATSPEGITARDSVDEDASSIIDGPVSSDEGGSVLVAGPSGAAAAAAATRAPRADPDDDPSAAGDASVSVAVAETRHVGGTPGDLAESTARSASGLSNGESNVGYESVADSVCASQHRQKHWVNWMAQSVGMSMVTSVANSSRAVFTGYGRSAGGPMGKACVPEPASSNLSVNQRIPRPVVEESSNGLYRQWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNQ6</accession>
    <name>A0ABQ5RNQ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="9">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_000592</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="9"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18015">
        <property type="entry name" value="DEXHc_RecQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="103"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="301"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="613"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="721"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="25"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="634"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="749"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="771"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="822"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI58828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="853" mass="93094" checksum="4F468D7ACBBA33DD" modified="2025-10-08" version="1">MAEVEDLTYEGGESDEGELPLTKQLEDIEEELSEVKSQIEFLIQQQDQLRARRDQILRQIDQERRAPKADWCQENFPWSGKLTQTLHDVFGLREFRPLQREVINATLQGRDVLCLLPSGGGKSLCYQLPALVSSGLTLVVSPLLSLIQDQVLSLRALGIDGACLTSLSSKEDVAAVYGKMDRGELKLLYVTPEKIVSSKRFMSKLEKVHQAGRLDRIAIDEAHCASAWGNDFRPDYKKLGVLKQQFSQVPILALTATATHQVCEDLKTILRISGCEFFRASVNRPNLLYEVLPKPAGASDAIAAIVAWIRKHYPRGESGIVYCLTRKDCETVASELAGGGVSARHYHADMDPGPREAAHAAWSSGEVQVMVATVAFGMGINKPDVRFVVHHSLSKSLENYYQESGRAGRDGLPARCLMFYRFSDALRQAAIVCFEPTWQPNLQAMMKYAAAGPSGGTDGSSSCRRAIIQRHFAEAPAECRCMCDNCCIAAGPVTSKPRDVGRHALALLGILRQQQAKEKKATLIQLVDLWRSCKDPGVGKEAKAMSRDEDEAMVAAMMYAGLLELEFGHTAYATNTYLRASRRGIQLLEASESGSGKPLQLLMPVSQAIAGPGGSASVGRKGTAAAAESAEDVRADDDDENDGADECGCGDSVLRASLERWRAQRAADLHLFPHSILSAEQVVALSAIQPPASTDALRAAVGVRRYELYGKELADVLEGRYKPPAARPPPEPQPAAAKAAAKRARDGTTRASTDVPTAAQQKRQTEADVVMLEDDSDEEMEKTGAAKRKKNEKNEKSGARATSGRQKSSRLPALKKVANDSGNDHSDNEHGGDEKDDDEDFETNVPLAKRRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNQ7</accession>
    <name>A0ABQ5RNQ7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="453"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="728"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="818"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="983"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="845"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1211" mass="124444" checksum="508DA01B2E3C7BA5" modified="2025-10-08" version="1" fragment="single">PPPPSPPPHPPPRLNYHRRSEGDDEMELRERANSRGSVNGCHRNTTGYEAPRRPPVAEQLNGVGGSWEAVPDHRRCGRSTCRREHSERCHTTPRQQESRDRHRHHHHARIHHQPGHSHHRRSHSTSRSRGKCLDGVGVTAATCNPSMGVAPVAAEVEAAALSAPVAENPIDIASAETGANTEPSDVSSQSAAQVATLPRPLVPQLSRLQLQGSGANASTEYVRATLESTMLRIGGGAADAVAESTSDAARPTALSSTMAAPRSAPSLPPVAPATASLSMSSVVTSAPVGTAVASMPVIGAFTPGSTKTSSTAPLLPQYAAQAVVSRMVEFGEERSAATEAEPKAMLVSEAANAAAAAAAAPGVVEAVNGAAAVPGRYPRKESEVEERASVPLPVSLSSPATCLEALNDKVVDGKAYEITQLAASDGAENAPRQRSVSEDHLLASAGPVTEAAELGPSAADARTDQEVDGEGVGAAATQPSRLCPVAACGPPPPGRLTAQPQQPLPGFGASGDVSDVGITRASEVKCPRSSAVTVVAAGGAAAESPEDVLQRISAGGGATTTITTTATTTTSPRSCSNVRPVVPARLIAPVATAAGPPPWYGAPPADPARHEGLVPHLAAAAQEASMMDTTSSQPIQVPSLQVIMTGPTTVVAAASPPTTVATFMEAEAVAHRNSSLLRLLSTSHQYLQQLPHMQPAHGPPVVFGLEQLLSRSATAAPTNRRDDAIVHPETALGDRGAVTSSSPQEDFTWGEHPASAAGYTRGDYSIRGLSSRRGSATGRGGSDNGSGTGCAPLEAAAAFTAAHSGVSCGGDSAARMAIATKPPPLPTGQPAAQQQFHHHRSLHSQGQQQQQQQQRYEVPDAQQHYRHPPGQQHGQEEQRPPPRCWPANSSNLGVGAPSWQLQGLHHAADAADVPCAPDLSTTCRAAHLGYGRVAQDYSNSSGSREKDAGRDGKYGHLTLVDGLPAGDGASTLMRPVISQLQRPLAGGTAARGMPGHAAPGSSAPPPPLPPLVLPNAMTTGGGTVHVLPLAGGTGVEVTVKLVGLHAVEPAVPSPSPSYGEIGPGRQLEKQHFPTQRMLVNDSLVPGAEQPPSQGSGSAVAAPSPVLVKVRLPPGRPPPSAHQYSQQPGRQAYFGNVDVGAGGQAEIASLAGATAGARGSELPWLGGALPPDRMDKRHQGYVPNGGTGAGAVRVDLSHGRFLQSMRARGVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPX9</accession>
    <name>A0ABQ5RPX9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_001227</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNU66/SART1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045347">
        <property type="entry name" value="HIND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005011">
        <property type="entry name" value="SNU66/SART1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152">
        <property type="entry name" value="SQUAMOUS CELL CARCINOMA ANTIGEN RECOGNISED BY CYTOTOXIC T LYMPHOCYTES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152:SF5">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19252">
        <property type="entry name" value="HIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03343">
        <property type="entry name" value="SART-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="75"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="264"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="371"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="514"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="782"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="382"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="407"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="514"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="863" mass="92211" checksum="599ED31D61201142" modified="2025-10-08" version="1">MEQDGAAGGEISLSIEETNKLRISLGLKPLRVDTQPNQAEKERQNHEAKKREEAEAQAAELRRKIEAAREKRIMESKLRAVRTLGEADDGEDGDMATWVEKNRRLEQQRREEAARKSSAVPPNKRRQRDSDDEDNEGYRATELEGLKVRHDLTEVGEGETVIVTLADKPLLDEKGNLREDEDELENTLLAEEKRRRKALKAAKKEAKPLWEEDGKVRTLLDKYDEEEEEVVVQLGAGGRLERDRLRRQEDVRKKLAEGNAILDSVQQRQGTGAGGDYFTPAEMAAFTKPKKKKPKSERRIRARSAEPAEGADILATLEAQGEEVGVSGAGDLGRKADRGAAAARAEAAARAAAEEKRARFDAALEKANWASQFLRPQQKGKSGSAVGPTATAAGVGPAVGDDDDGADRELRESLERARRLAQKKKEEDAARVKTEEDGDEGGGEGAAARGSSAADSVEEVARLAAARREQEGKNKGIGTLFADVAVIKVESGTAGGEVLTETMEFVRNITAAPAPAAAPAKVAAAGGSSPATAPAAPDTSRPGAYADGEGSDIDDDGDARMDDDGGDGGPSGRGRARSEEPAGPRTGTWIAAEDMDVDETKEAGGSRRRGRELSEGPSGGQERTPLELADVGITGERSVGRGLAGALGLLNDKGALKEQVEWSGRNNDKSKNALQGLDDVFTGGRNQDRVARSVEAALTIRDKYGRVLTPKERFRQICYSFHGIKPSKNKIDKQMQRDAEEIAIKKAATTEDPSKELDRLRAVQRVTGSAYVVLSGKNAGLPAVLPGSAGADGRADSDDEGDGTERRRKPAKGSMPPPGGPVHPTAGVARGGGVGNLTPLLGDRKVEAMLGIRKPPKAPGAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ17</accession>
    <name>A0ABQ5RQ17_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001594</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045512558" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="27"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="1020"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="971"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="975"/>
            <end position="1001"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1166" mass="121338" checksum="DE900828D83320D5" modified="2025-10-08" version="1">MSYIWFTLAAIVMAATLGRGANTVNDFPYCSCQTYDCECSPYTISLVNQTAVDQNTTRFCFQVGFSPCNLGRPCCRGLMASVDKLSFSIRPECQKEKLQRITVGKQKMISWNSWRSYAHYNGAGFDYELKLYDLNGTSSDFPGTRICVFATAPCSTLQQLCKGPNGLCQYSFAESKDTFYCPTCTKNVAAAVPQLLFPPPLPPAQLLPTPPPRSLPPKLPSPSPSPPPAPSTRPPVPNPAQRPPTTSSPSPIPMPPPSPSTPRPNPPNSPPRSQPPPPPSPWPTSPSPPPPRSQSPSPNPPSTSPSLPSPLPPIPRPPSPLPRGPNPPNPLPPTPPLLSPLPASPPPPNPLPPSPPPPSPLTPSPSPPSPPPPSPLPPIPGPPSPPPPNPLPPSPSPPPSSQSPPSPPPPSPLPTSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPPPPSPLPPSPLPPSPLPTSPLPPSPLPPSPLPPSPLPPSPLSPSPLPPSPLPPSPLPPSPLPPSPEPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPEPLSPPPPSPIPPQPPFPSPEPPSPDPPSPEPPAPLPPSPPVPKRPLSPPRRPARPPPSPRPPPRPPPPQPPSPRPPPPSPPSPSPPPPSPLPPSPEPPSPLPPSPLPPSPPSPRPPPPSPPVPRRPPPSPRPPGRPPPSPRLPSRPPPSPRPPPAPYQPVKMCFSISIQNQPDPVPDKPFVIPTDSCTQLQAAMVNDFMAAVLMAKITLVSRFTADLCDSSTLQVCAAFRNPAATFAIDDEAVILGAKYMAQILGYSCTDQTRGYTLLYQASAAADSEASISSNSFFYQSMYQCSEPTPPGIARIATEAVRPLHLSLGSLVRTSGSAWQWQPWPPSPPPPPPPPPPPPPVPHLPPPLPQSRLPSQSSLASLQLPLPRCAAKPRNTPFRFSRLSVWSALGHDDAVEICTTLKARLCAGSCCQMDISQIAIPISNTCTPQLLGATVNDKAVTYSLARVDSGYQELQVTGLDSQLSPGSMVCMLVAAGECSSSSTLCGEDGCLVKAFSNNGKCCASTTIQTEASLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQC3</accession>
    <name>A0ABQ5RQC3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001709</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="624"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="919"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="961"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="992"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2000"/>
            <end position="2021"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2033"/>
            <end position="2055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="825"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1156"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1306"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1428"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1516"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1683"/>
            <end position="1708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2153"/>
            <end position="2250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="832"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="847"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1156"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1309"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1367"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1386"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1434"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1518"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1684"/>
            <end position="1707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2154"/>
            <end position="2167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2173"/>
            <end position="2205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2220"/>
            <end position="2232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2250" mass="244061" checksum="BB21B3F1067F4DEE" modified="2025-10-08" version="1">MGQGLIEGPIPSTWLMMTKLRTINLTGHPNFCKDWHRIVSFQIQSYVFLTPYGAVWGLPKRYYGPINFGNKGFQWNITAFNMDGLGWQWYDPSTREAGYTNVIAPDGKCCWDTFSDQFAARNYTLEGPSGTSGPWDYYGLSYERAQLCEWSAPYMPPSAASPPAPPVAPMPPDVPSAPPVPPAAASVTIVSPPRPPLFPQLPGISPMRPYAPPQPLHPPSIPGKWAGAWSWRPNYPFQSPSSPQPQPQPLSTAIVKPPAPQSDTPIECTMYSNQTIFAIPAISTGIFYVAIAVPEASYQYCKFCGCVVEYIALQRGSSSYYRELTEASKQVNSSSGTSAGKTVVGDRVGTDTINDMNAYSNSTPPPASKDSYVLTIISQGFTLSRGELKEVNINSPAPPPRGSGNDGSSSELQRTGPGLGGAWSLDPGVNGDYLFKIQVGGNVWYRWVLVDVDPPFVSGSLLVLNKKATFEGSTTTDMGKGRWQYLLVVLNMSEPVRNFNLMEALQLNDSTLIRIDCFESLDTASIKVASTSYKMVESVGLPEASQSSISASQETPLSDYYIAATYGRHYVRSCLAVLYAADDSKPSITLPRGAVVDLTGNPSVTSLFLQANLKDSLSASADRLAGVTSALVGGILASTAAIATSAACISSVPSHSSLLQSAYHIQMLAMSVNLASRGISEMYEGLPEVLRWSLMGIQGHLPALDRMFSSNASTNTTDSVSKVLGLDMNSLPPSPPASLAVPSHNRSRLLLADVNASLGGLPPKPTLPPQLLITQQSQQLPAMVQQTPTADPYPPSSPLDLMAVTQNVLIEWLQTMMAFNMLASSSNHSEESNTQQQGSNSTQQQEVSRVSKNGSESSGLNSYVIVGGGADSINDVSIDADGRVMNVSWIAPAEESPTTSSINTSATHERATQRLTSTLANAGLVIGSVLIVHLILILLYNRFVGGELNPILEFPRVEVDTAGLILVALTFYSCCTFGAFDKLWQTDQITAVAVLLLLVLPYLLLLWWITVCRWYLEETPPGDRCGARHHCEAEGQQQQQQTGPGNIVVASGTVRVPDQEVKDAEGYEGHRRTARLGPQWPLIHEVNAFSTAGTPNLSNSTTPKGREAQVPARAPTALRFGQNSHFLLKIFKGSKFKNQGVQSPHQQPAVLSQGVQQQQQAAVQASTVDGSDGPGYRPCRTSSISSRQKPAAVMNDQGPSSMDGQKLLAPVAESVLRGQENAVSRTGSLIFGHKFRMGDVSTVVPEARQLTTRELSVTNKIAGRHLSFSEGNYRAFVQQEQDAMNSSSAFLRLRQHSLVRPSEIAPLVQQPQQGHSSRLSVPSNCTRSLRPSLRKQVNFDADEHMDGDGNSFHLPKRPIIPTRRNLNRPDDDERGSLSNQRKDNADAQDECCDPTEIEDYDDDEPGTQLRFSPLRFVHFDIRPPLPHGTANGLSAAPSPSSASIIKGRDAPAWNLESVKGIRSSRSTTLESRELKPAIWKAAEPHELALSPTTQLQDPVPDEQQQQFSIQLPAAAGALRKERAEEGKAAGPAGKSPLNRGLRILSNPKQSYPDLATDGEDSRNHHSWRPRPTSFLRPTMSSQVDVSRLRSFSGITSTHASVKSLPIATIEALESMQLGFKVQLPADNDSLGQQLSRGNRRSVLTPAAGPMAEAIEVEKQNGETPAIGSDDPCFLLKAPRHKISASPSDQDEVPSTSHPRRPSFNNSRARPISQFKKFTHFVTAVVRTPGMPVMVLDAPATSSGILPSLDQANFGMYALTPGVMLGEHIVAGTDRCKNPDMVSDQACATRDKSKDHRNTNSSGNAIGDMLWWPAVAQQEMDASHARPVCESLETPAASRLREDSFMLGNQPATVDKRLLALYPGKMVPMYLMRYNPDCWPLRLCIAPPIYLLARFEFMFEDAISRFNAGSTRFGRYRIHSPTQQCRRSTWSMGGMQLGRETKMIMSINAANVTHKIFCAAAFGLLGVYTRSVLQIVLLIVLQSAITLQLLAWRPYINRAQLLSETVCHMGVMGLFLCAAALLNREPDNHASTTWIMIGCFFLTAGSVIGYTMYTLWKSIQSITTKVLKWLKDRLGGVRQPSLTLNGLAIDNMADDNNYNRQDSSIIENAGEQISAQRSKPMTLSYVSHAWSPAEGPNASVELPSLSRVMMFQTRRSSDCDEGGRRDSRPISGVLAGGRDAGGGSGVLGGGRDAGSGSGVLAGGRDGGVSVVRTAEDEIQGRSESSSGRNNTELMRQKTGSHRRPRSSIVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQX9</accession>
    <name>A0ABQ5RQX9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM227 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029417">
        <property type="entry name" value="FAM227"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14922">
        <property type="entry name" value="FWWh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="99"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="338"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="382"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="704" mass="73175" checksum="580A65A914B4A191" modified="2025-10-08" version="1">MSSALAPQTPSATNGENESIIQLLEHFKDDDGRVALLVKELQELSNRVQVAELATVDPRLTTAKGRQGSGTESGSPRGSSGERLRGADAFADAVNRARRTIYHGPKSQQSSRTSGGTGEAAGGAGGRGQVAAAGRVEEATLPDPPVDPSQPLPLPPGGHEWEALQAAVNDSVASFGAAAAKMGWKKWALTRAHSALLVSLFWWVVAAAFRADHPLSRQLQDFAFGHFCRHYVTVVLNLRGKARDQYQQVWIEMMAAAALYLLAEVFPRSQHKFDEGLRGLITKQLRLWTAGALPGDMHPSAHADAHDASPAAFSYAAAAAATAPPPAAGNGYGGTGGGGAVSSSVGGQSRTLTLARSPPVTEAQVPTFNHSGKSTPHRRASSTRLSPESSLRNSVSSRTALGSTTAPSGVGCGDNWGADGGDKGSGHGYAGPQAFVFTKNSPLMVQMLVGANGSSTAAAAAAADDSGGSPAAPPIIKGLMKHTIGSTLARLHRALDEASDEELSYSRLAAAASASATDAVTSYEASRRAAAAEAAAMRRGLVEARGAIEARQRAALAAGSGQLKQMSDRLSGYLQNDVDRLMAGKPTSTEPASGGDVPRYLRPTAGQCCGGAVGESPSIKPARLMAPLYEMRRVEGNIRAALAGGRHSSTDTVARVTAVTAAREAANAADWRPLAQYPVGTKDTRRRKMAANVLYGLDLERYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQY7</accession>
    <name>A0ABQ5RQY7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="517" mass="57979" checksum="A555C78E4648551D" modified="2025-10-08" version="1">METHSPVVCKLLVCCPNEEKRRIDAEINLSVVDFEQLLTDAAGAKSISEVSVQVFDSEDEDGKLVAQQVAPVQLLSRCTPWLKKEISAPFKGKAWNQAQKKLTIHVSLKQPSVQLVSPEVSRISIRKDHDRGEPLKRSRSYEQAAINKAVRDNVPYNLDSSLKNPRVLIAKDIQREPHLQPVWDKCFAAGFAQVADLQPRVTVDCFKKKWQKYATDYNAQRARYEKVKATHKEELACKPEDLEKKPWKKLWNKESRAAWRSFKYAQPDNPDAGPSERRIAMEDLNVTDRQQELINALVQRKQIESSKTSFTVPATPVPVGAGGTAQHTPGLGALELPKGTTKSGRNDEPPGTQDGRTSQRQKQPAHGEDALMMVLAANAARIHQKEKEIKAARRQQAKSSEPSKARNSSRNATNSSKAAAKKVTNNDDVRPSMKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRRKKVKSASEDVRCTRARRQHGSSESKKKQELLQEEVSKDEKSEDEQDDATSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR27</accession>
    <name>A0ABQ5RR27_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002053</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="184"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="252"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="319" mass="35409" checksum="51888C0C1218F5EA" modified="2025-10-08" version="1">MGCAGSRTAVFVKESPLTEALERKLVEAVISTKRRKSFVVKKSSFNNLMLQMPKLTAGFKTIREAYVAICGPSGKVTWGTFCSASGDKLGLEPNSESMKEVLSIPDVADGVLVSHPDLILFYTIIFLLDGDTKRRSILSHKVHACLDIMEKSFMFFDSSADGLIERKELAHAMKSGTRVFGLRYSKTLADRLFDQLDWSRDGHITFKEFLIGMERIIIETAGDDEEDLGDEEEDLRDLDADWDEADVKQMKVTQTQTQTQQKLVSSPSEEAAGCRKPEQQQQQQAEDKMSALGALEREPVLGGSDEVLPFTISATGAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR92</accession>
    <name>A0ABQ5RR92_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_001725</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. UDP-galactose:UMP antiporter (TC 2.A.7.11) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF8">
        <property type="entry name" value="ADENOSINE 3'-PHOSPHO 5'-PHOSPHOSULFATE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="337"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="337"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="377" mass="40079" checksum="2DA4EF8AFAAEAB1C" modified="2025-10-08" version="1">MVKEVHDEGYAVVARSDVEAAQAEASSKPAHQQKPPRSPTAFFMALAVGSIATALGFSTLQEKVFNIPGFTYGGWMTFITYLTYSACGLAESAATRSFKRNASLRDYALISILAMAGAYFTNWALNYLNYTTRIVFKSCRVIPVMAFRSLVVGQRYSALQYGAGGLLVVGITLFTAGDAEGTPNFSGVGVALIAVALLCDALTANLEERQFFRIKTPASHAEVMTYLSMFAALESFMVLCVTGELQAALVHSSQHRETVPTICAFSVLGYVTVCLVLLLIKHFGATNAEVVKSMRKVCQVILSFLIFPKPMSWKYLAGGFLVAVALYALQRTGKKPITDGKEKDKEGRRAAVSAVPGGTGAGSGAGDHAAGGAVGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRM2</accession>
    <name>A0ABQ5RRM2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH:ubiquinone oxidoreductase 12.5 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_002313</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA8 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016680">
        <property type="entry name" value="NDUFA8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13344:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13344">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="12403" checksum="2AC998B93C778AE6" modified="2025-10-08" version="1">MSSPVRVPYSHELYAMSRHIVFRAEKEHKAAIDCKGKHDWPEDCKPESEALVRATNKLYKEIMEKAPNEFKEYAQCLDWYGLRFARCRDKQAAFEKAFPIVEHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRY2</accession>
    <name>A0ABQ5RRY2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002467</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009244">
        <property type="entry name" value="Mediatior_Med7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044888">
        <property type="entry name" value="Mediatior_Med7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428:SF11">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05983">
        <property type="entry name" value="Med7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="244" mass="25636" checksum="1BB3A7A92E7384DC" modified="2025-10-08" version="1">MAAAANTGAAMREAYPPPPSLFNLYGPDDGVSPLPPPPPPIPTPADVAALRERKIELKVLGNPLKLSEDLVPPLTTQTLYRHQSDGSIDFKRELRRLSGELMFMFLELLKTVVEKPSGYAPQLTSVNVLLSNLVQLSNLMRPYQARATLEATLLLQVATMRNSINRIRQQVAAADEVLAGMARALVDVEGDTADSAARPSGGVGPGPGPMAVPAGGVGGTGTRFGIGSDESLAGTGVIGMDMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS24</accession>
    <name>A0ABQ5RS24_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="307" mass="33992" checksum="81328954BB762D91" modified="2025-10-08" version="1">MHVPFVSTGHRFAASLRPSHSHQLIRAAVPQGGQETTPETQYTLTAASTPPPVKATAPDPRPFFAAKTPPSQAPPSARTSTPTPPTTSLPATAPAERNGELLARRKQRVTAEIVEAARKARVAATPEQISAGLDTLESLVPGFTPNLDTLKASEWARLAADAPAVASKIILLKSHYPRLDLSRVLVAQPKLLLQSPEQLDRSAQQVRSLLSRAKDLERLLAAEPALMEPRALISVLITVTKWYYLEKDPIEVLEADPDLVRRAQDYDVPFEPVYMDEKGNWTAPLLNYQEKRTEWQKYIDQTFYKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS88</accession>
    <name>A0ABQ5RS88_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="197" mass="21697" checksum="77F7828C2EA87D14" modified="2025-10-08" version="1" fragment="single">QIDRHLPLPTSATIEGGMTRQQLWLVQAPPGCEQAPWDVIALAALSSMDYGRHCLRAATREHRPQGPSTHGRQQQQQTHTAEQGPRQQAITDFFNRSGGFPQEEEEDANTHPIISTTQPAMDTPTQTAASPLDRARARTFADFWARLHSFAALGIPHYGWDQVGPQHPILAIVGTQMRCADPVDITDHGAARTEQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSM6</accession>
    <name>A0ABQ5RSM6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="696"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="781"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="889"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1075"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1125"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="508"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="810"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1076"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1126"/>
            <end position="1139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1294" mass="138626" checksum="BCAF0060A36090F6" modified="2025-10-08" version="1">MQICYEMIISIIHIGYRTWRWTFTFVVSAQYTAVRIAISALRGLIRLVPRTAYWILSRFGISAKRCRTSNPTINVVVHNQGLPGADISIVSTGEAIPSQAFESCIGQASEGKWEPTDAVPAELVKLRLVTDSVGNVHVSVYVPTKRPNKDSTTECDTRRSNSIRSRPSTSSLRSSSSCQLPPTDDITEIVPQKSESVVMIVTPHLLRAPAAEGPETAATFKASSFGRTTSACPTAQAGVLITTAGSVDFDEADETSKSCDCWCWPSNVLKMLRQQHSPSRVLPLSLHKGDPVVDERQEALSQPKPSSSGEDEELEPLLLPLTSPTPEPPKAQQPSHAQQGGEEHQVSLPTLKMQTSAGTPSLQDDEKSQMLPLTSHPVSCKPASITFDGPSDVPCYQANDETQLEAHQDKLPSGLTRTPQPAGEPTNSTQIPLKLVDPQMSELEESWGLLEQQPVLSSPPSAQPAAGGDELKEEQKSSASSAAEVEQQPVLSSPRPPGGGDELQGGMVEQEQQQEEPAVVEELKPASIDVVELLEEEQQTSFTVQQRISLALDQQLLSLSLVELQAIGSLVVKQQQPHPTESAPSPPNNEVHQLQQEQSRSVTMTEEKPKPSSLSSTASTCVKEKQKSLVVEDQQPRPTIGVKLLQEHDDDVFKAVEKFMTQSSALPAAGQQEVRASTVEQLLLSLPAVAAGQQTSPFLQPAGDDFEAVQVPPPPTSSSSSISLSERHHQSTPLLSPPAETAEVQAPSSPITAGQKHVTLLRRRQRWLLLCRRVLPAAPAVTQRPPRGVADEQSSSAKLSLPDVDEQSTPHHPSQATDNLASFPPTLSFEIEMWDPSSEAPSPPAQSSSVLLGMNLDLQEPSHSQPPQGKESLPIPSTLSLEVKILEPTSEVADQEQTESPPFNNGDDGEADAQKCHTLPSSTLALEVQMLEPFLSSLPSSAAERASLMPAAWSLELKLSEPALSALGEHEPTPWPPSPEPYQVWAPSSPTVEQNPMSMSLSVEVKLCRPASSSSVAPPVEQLEAPLLLLRPRSSSSAPKQEQESSLLLEALPAGEQQSAAAGVPTTLSLEIKLQQEPSKSCSEEQQQQHPMPSTLSMQVKLWEPSTPQQATLVEHCCSAVQSTPQQEGGELLQESQQPSEERVRSPMARLSPALRSVIAQMCVSMLCMTIIPRVLPVGGRLLAYVSNLAPFRGGRQIAQLAAGISTEVAAAAGLQTHQGVVAVEVGAIDATAAGNKGTAFISSSPAGANRGVASQFDLDNAFSECNSKWCVIVRPPGGTVQPPQPAAAAPSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSP8</accession>
    <name>A0ABQ5RSP8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_002766</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0229">DNA integration</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="11">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI60618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="178" mass="19973" checksum="A47B79C4D86BE227" modified="2025-10-08" version="1" fragment="single">MLDDYSGISVVQPIRLKSDARDFIKKGIALLETQCGRQLKCIRTDNGGEFVNVRVKEFCTQRGVKHELSVPYTPQQNAKAEHLNRTLLDKMRTILAESGVPKKWWHEALCTASYLRNRSPSSGKPKTPFELLIGTVPDVSHLRVFGCVAYIHVPKGQGDKLDPRARKGTLLGYEPNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUT0</accession>
    <name>A0ABQ5RUT0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Golgi apparatus protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_003321</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001893">
        <property type="entry name" value="Cys-rich_GLG1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017873">
        <property type="entry name" value="Cys-rich_GLG1_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039728">
        <property type="entry name" value="GLG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11884:SF1">
        <property type="entry name" value="GOLGI APPARATUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11884">
        <property type="entry name" value="SELECTIN LIGAND RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00839">
        <property type="entry name" value="Cys_rich_FGFR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51289">
        <property type="entry name" value="GLG1_C_RICH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045750663" description="Golgi apparatus protein 1" evidence="10">
        <location>
            <begin position="25"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1130"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="97"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="153"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI61059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1172" mass="124281" checksum="D5AB43BF68AE97BA" modified="2025-10-08" version="1">MAARRLWSALCLLAAYCTIAPVGASLVNAMLGDIHPRSHRSIQSAAQEVSSPDVPLNGPCVADIVQHCRHLLTEKVKAAISTKANSEQTAAAVGSSGAAAGKPINGNPNVVQQAAGGGANGQKNASGGTTTAIASGAAAAQMQQATVAEGAALGSSRSVSGGTAATEKTGGEGSGSAVPISNSSASNDIKDAGAAAERTSNTSTAVATLRPQQQQQVVSRRRRQLLAGEVDVAAVAAGNAGSAVSAELNAAAAHSDDLDQALQLLGLRSELGTRAMAAASAPVAKCLLSAMHASEMSISPGIPSVRDECKAEVRSVLIQRASELRYDPPLLAACAHDIVTHCGDVASDSVAVIRCLRNAKQQLLPLCRTAVTARQAAAAEDLSLDPELQRSCGVERDTLCLEAGWGEGAAQACLLAHLRGSLPQVFSEIIFPAIGAGGGGDSSSAKRSASSSNSGSATAVELSANCSAALVSRLMAEGEDIRFNFQLSSTCAGDKQVLCRDVRPGGAAVLRCLESHLDSPNMSEECSLALGEVRQLRSLDVRLDHTFVANCADDVRSLCDAAVAEQLGSGPAEVPFGLSAPFECLRSRLELVRDSTCRRHMYESIVAAYTDNRLDAGLMRGCHQEVAVLCPQHPPRALECLQEKIDKFSREEAPAVLKGKVSEQCMAVVLERRTQAATDVAFIPDLMEACSKEKATMCSAPGMEGVRALDCLVDRRNVPGFSERCGEALREYLTEAARDIRTMAGLNKDCGPEIRTLCKGIQPGEGRVISCLRDQRANITGETCRQQVMRLLGFIVEDHRMDVGLTEACTSDVQKYCGGVEAGEGQVHDCLRRSADHLSPECRAAEEEVERLEHEDVRLNPKLMRECPLAVSGFCADVPPGDARVISCLQTNMDKGHFPAGCRLAIRQLTDRAAVKYSLNFRLRQECDDDAQHLCPEAVDELGSSRAAITGTGGAHREETTLACLARQSMQLAPGCKSELQALVKLSLNHYRVGMPLTSQCDGDVMQRCQVDQVAAPFLQNGFVLSCLAKHAAHLHKPCWELVSTMDEGQFKRAVAAETTAWQQSQSQSQPGDVSHLDEKVLERLVADLRKELEPRIYSNVHEQINRKAVVVARNVTGALVMTIAPRVNALLHTTTWLLGLTALGLLGGLLAWRKFLSRGGNLLVVRKDGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUU5</accession>
    <name>A0ABQ5RUU5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045131">
        <property type="entry name" value="CISD1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13680">
        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13680:SF5">
        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="101" mass="11098" checksum="A08AE027A7373386" modified="2025-10-08" version="1">MASLRAATVSRGCMAVSSRRPCVFVRAEGQVNPSIRKSEEKVADFVKASELPKPKAVFCRCWRSAKLISNTKRRACRDCGSCLSPDSLTRPGRTKAILILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUZ8</accession>
    <name>A0ABQ5RUZ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="1">
        <location>
            <begin position="91"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="165" mass="18807" checksum="E653A3027E00F84B" modified="2025-10-08" version="1" fragment="single">RCVYLRVPGHVRACINKILTSREIAVETGSEWQPRCYKCKQMGRLRYECPQLRNQQQQQNNRGGRRNNFVMISTAVELEQARDAIQEYADWKLDTGAALHVTDRMEDLTDFVPVENGPTLEGTYNVKQQAVGYGTVELVAFIGEEVLSLLITEVWYVPDLGHSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV29</accession>
    <name>A0ABQ5RV29_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CNNM transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_003332</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="11">
        <location>
            <begin position="20"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="222"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="334"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="421"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="910"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="955"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="340"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="472"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="495"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="533"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="914"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="963"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI61069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1024" mass="104477" checksum="806036BEB7F5F4C0" modified="2025-10-08" version="1">MYGFIYRSLLSGCDVEVAEEHKFNALYLFVCILLVIISGLCSGLTLGLLSLDIVELEVLKRSGKPREQHYAEQIMPVIRNAHYLLVTLLLCNAAAMETLPIFLDKMVDPVTAIIISVTAVLFFGEIIPQAVCSRFGLAVGANLAWLVRGMMILTFPFAYPIGKLLDFLLGSEHHTLFRRTQLKALVDLHGADTGLGGKLSKDEINVITGALDLTHKIAFRSMTPLDKVFMLSLDETLNDAVVEAVLESGHSRIPVHRGTDRHDIVGLVLVKELLSHVRKGGQDVPVSSLKMRTIPRVSAATAMYDMLKLFAIGRTHMVVLVQPDEEQLEEILDEVAPDRSSSSSSSSSSSSSVKISNRSSVDERLRLVPEDVPLSDAAAVAAGFGAGAASSPFIGETTVASASASAMEQGYAHQLTAQWLSGRPESPSAPSPAHGPVIVDGSASPKPGGPAGGGSSIIRHSIKTVKTFVLGGKDRNGSGGGADHDNVSHHHGHHEYHGNDKKERNREDKDKDRDREGSSHRGSAFRRSRSRRTSSSSSSSSSSSSSSSSSSSSSSSGDPHSHMRTGSVALSDNAPLIAAIEALENQLEDNRVLPGQPIGIITIEDVIEELLRVEIMDETDQYMDNEKSKPAMDAKQEMSMALPERLQAILGLFSARDASNNLARISGMRHWQTIEAVAAAARPTGTSAAPGLIASAGGGPGGGGGGIVLAMSGAAAAAAAGGATGGGLVHSASFHTTNGLSPALPAGVASLSSSVIGGGGAGGGAAAAAGLAPSLGSSSAFRRPAFLQAILGAAAVGTATPVQSSIGAGVGGIAANVAMPSSNSIASYVSMPLTRHKRTSSGNMLSNLGSLTAGASGGGGGGITGKSTTASSSNLTAIASTAGGGGGGGGLTLSGMGHWLFGGGGQNAPAHTGSVGGHTAHGGGGGGGSVASPLPARLVVGHLVGTTSQLGAITASLPRSSGRHGGGSTGAGDRGTGDLEHNSVSIAGLSRLGCGTTYTGKVSEENSRPAVRLGPYELARTNSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV66</accession>
    <name>A0ABQ5RV66_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="180" mass="19622" checksum="C9D7E5DE25D150DA" modified="2025-10-08" version="1">MSGIGVGLEFVKRESVVVTSVDQQQDSCLEPSTPTSKMNYSTDEVYQQQDVVNCTSNRTGTEIFIFPGAWALGEGLFTEADIYYKQQQKAQHGLVPSSSDVEDFGTKALRLAGSLGENLRFYLSEFPGHKAASLCKNTSSSCLTVTVDLSPANSMTPASAESKAHSRYPWFVEADSKKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVL4</accession>
    <name>A0ABQ5RVL4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_003738</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF26">
        <property type="entry name" value="OS04G0435100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="505"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="643"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="647"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="672"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="868" mass="92086" checksum="D2ED84DAC386296D" modified="2025-10-08" version="1" fragment="single">MNRSKSRSDKDGLHASSFRRQGSVSRGAPPNPIRRRANTDEVKLLMMGYRQELSREFTLFNCFGCSFTALSSLTALGGTYGFGLTYGGPVVMTWGWLVVTLFTVSMGLSMAELASAYPTSGALYYWSFKLAPRRLRNLACWTTGWILTLGQAAFSASNSFTFVHLLCTAIKVQFGVSLKSVHQLLLLLGSLFIVGLLNCGSARLTAFVTTFGTFWHLIALAAFCVTVPLLAPQRQDAQYVYTSWQPHHEYTGISSPVYTVLVGLLMQQWTLTGYDGAAHIAEETQHAEVNVPRAILLAILAVGITGYAFVLSLLFVRLDYQLLMDPANETDGGNVVLQILIEITKSTYGSASAGVALFTIPIVGTFFCSYQSIANNSRMLYAFARDKGVPLASFAKLVHPRTKAPIFGVAYMVVLTALLAAPMCYNDFVFPAVTSFAVVGCYLAYSLPVLCKVTTGVRCFLPGPFHLGPRLSYVNNVVSLLWVGTVTVLFTLPQIYPIKPINMNWSAPILGLAMAFALGWYYFPKYGAKKWFIGPRANLGQFNDELPSAAAHGAAAHGAPQNASAAELAAAAAATAAARLSLVHEEPEVEQSDRVMTEVGAPTPAAAASASGTANTHILERRSRSNSCMPRTSQHQLQLLHAGNKPSRATPSGSTAAMLQAPKAPGDDGPVAATAAATSAATEGIAFRDPPGSDRGNSSQGNAQHSGSLGNRGRSGGLGSVCLGSPQHRGGFISSTTTARLRRQSALSLVDGPVSCGSHIVHLVQDSGLLDESAFQAIANAYGTGERRSSTATTMWALDGRPTREHERGAARRMSMINSRPSPGGGRMDGPWAIWANWTAGGGGGGGGGVEPCDGNAVGVSNVSWVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVW5</accession>
    <name>A0ABQ5RVW5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hemerythrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003876</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12108">
        <property type="entry name" value="Hr-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="31716" checksum="4A6C6516C57FFA6C" modified="2025-10-08" version="1">MKAALTRLKPALIPGVSLEFTRLDQALDALCMSHIDLLKTLQVSPAWPYPAALDGWKLAHDAIRYDMDAIMAALETSNSSVVTRRRPLPDWQVQLLKSLMRSFYHHVHRQIRHKRCTLFPWLATRVILPPKIMDYDEPTLMALLDRVRALCENLNPGASASVKMAVGELHAAFMVLRTLMRQQMEREEVLGLPLLRKFFTAREAAVVEKKRVSQMKFAELAWLLRPLAEAEKRATLTRLGIPGLVQTLVLLPALRRADRAVLRAHRELASGERLRRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW07</accession>
    <name>A0ABQ5RW07_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004302</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="41"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="338" mass="36977" checksum="3B051457C924C31E" modified="2025-10-08" version="1">MATNMMVLPMKSGPAYSQLPTTIVVNDAPPREILGIPAAIVAGVFYCSASASMVLLNKHALASFNFTAPNALLLFQCTLAVVLVKLCEAAGWVRPLQPLNRRLVTLWFPVTCIFVMMLGSGFYALQLMGIGMFSVWKQLANLTTALGDVFIFRRSYTWPVWTCMAFMIVSALVGASTDVRFTWAGYNWQVVNCLLTSAYALCLRNVMDKVPQHTTDGQKMDEFSMVYYNNLLSIPPIMVLMAVFGEYDGLMRQPALAMPAFQMVAVLGGLIGFAISFSSLWFLSQTTATIYSLIGSLNKFPIATVGILLFKEPTNAKNLASIIIGLGAGVAFTQYKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWN8</accession>
    <name>A0ABQ5RWN8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="395"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="429"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="768" mass="79281" checksum="BF95BC0A72631E4A" modified="2025-10-08" version="1">MQIPQDFLGNDDQKQYQAMVTQYGQVDCSLMDVCPPRGLSENCTSLLSPTGSNNEAIELNAPMLYISQAQRHSTGIELFDFQEGGQGARPRSLASLTCNGYASPSSGDSTPTSKDSEVCSITGYTAAAIQNTEDCVSCTFGATALSLDFSKDEQHPAEVFCGNLPVREASSGVERISTSAPGEDEDGPPYLPREIHPFDIQLARMLAAVSQATNTSPAQLLQVLLNVAPEDYPDLVNSVVESGSSCVHLLACHLHNLRTMAEEAIDDSSAAAAAAEGTGSSGGAPVTATPAVGSSGKPSATGGRKGGVGTQQGLSHESDSEDKTAQEALRASISDQYRRVVLRLVETALVLLNGPPSAKPPPPPPPPPPPPPPPPSALQNRNGGGSRVRTEQLGRSRSVPSSSAASVPSPSPPPLGAPTGGASRCGPGQKAAAQRSSLAQSAGGLSTTTPSAPASGPLASEQLLAATDSTLAARGRSLSAALPASVVPVPPAAAAVVRDRTVTTGSYTGTGKVAVAPQPLSASSGKAPTVAVSTACLRGTQVRGPEVNVPLQRLQMGNPSGEPRHAAAMMRRQQQQLQLQQKLVLQRQLQLELQRHRQLQQQQQQMQQMQVQQLQLLGLLAAANADAGVHGLPFDMAISPSNMTPSALASAQAEALSIASAAAKLMQAYDGMSSVNDVVVWQPADPLTALQWQLPAVPPAQNPAGLDLSFPSELNIALRQQTQQRPLVMMQGNLLTAGVAGSHAATAAVPYGGAPAYASAALSGAWMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY29</accession>
    <name>A0ABQ5RY29_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="462" mass="49537" checksum="1E6369D33D0FA808" modified="2025-10-08" version="1" fragment="single">MSGGSLRRPMGFFAPFNTWWSSFFLQNGRRPTKTDIEMWRAGNPTWRPELTLEDIIQHSKGRRDPEKNRQYFVEYRRKRKDCEKRAAKQGRAIERAAPKHFVAGEPWEESTSLEKADLESTDEEAAQTSYPRRKQPFRCCRVKPHEQDPRSQACSSPAPITPGMDGSSADPDVVEATTAAARAQLGDSDTGTANSLNAVCAHDYHYDTSGDGTKVLPEAFSVAISAPHLPVQSRADVPTRIPVRRTRGPMVETQLRGVTTHMQKRGHGVVRVLCRRGNGSSSALKSFCNLATAAATVASDTFSWAIAGPAPVAAPSASWYAKSSNVALGYMHLEGAANISGAGAPAVPTHLETPRVGLGCVIPAPAGAHSSTFSPPCLVAGKRSDSAEPSWMLMPASLAENGYLTACAPPLGVEAVSGLDAMATDISSLAFECAADVSADVAFPTLPFCDHRLHGTCSMDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYU0</accession>
    <name>A0ABQ5RYU0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL45</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L45, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_005248</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL45 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051975">
        <property type="entry name" value="mtLSU_mL45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28554">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L45, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28554:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tim44-like" evidence="9">
        <location>
            <begin position="143"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="296" mass="33867" checksum="6287FF04644CFC1C" modified="2025-10-08" version="1">MSQLHMALGVAGIGTALRNAFQQTGGWYSRPLGSLITAQPSTSFAAAWATSQTRSLAGPANFAGFDPWSFFDYRKFIYPDVYDPNEALLCNRYKTHGSVVERYVPPKPFSHWNPLLDPELDRRTKYFNFRLYTSFVCYRSLWRFSKADLQTRILTAYKSVNLALATGDMRYAEPYVTESMLLRLTGEVSRRGRAVRVEWRMVSEPQPHDIQLVSGAVVQNPLKQGRLHFVQWTARVPSRQVVAVYDARGNLIAGDPNKELDVVDYWVFERPIIKAWMVPRPGPQGADWRLLDRLQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYU8</accession>
    <name>A0ABQ5RYU8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1243"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1011"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1168"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1223"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1250"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1338"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="304"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="393"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="523"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1178"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1223"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1346"/>
            <end position="1360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1584" mass="164240" checksum="F79BF47F9F861055" modified="2025-10-08" version="1">MGGVCCKDGVDKNEDNIGGLPGPAETASAPAPAPDKASVQASGASEPVIELGKKPESQPVVKEFPTETRTALVPIPQPTAAANTIDAAQVILRNGAQNTAPGDAVEVQQTSTIRVDEVATSAAPAVPPTVSPTAVNSATFQTTTPASGTAPPLADAAGTTGLETKLKHHFPPSPQPELESQPQARSAPQTEFHLQSLGVTEAPLPIRSQILTDSVQASESGPRTSAAMLHVASTGAKATADTVALLAHRMTEAGLSVTRNSSVTTTQPEMETAVTDAADEEPARVKVETSRRTNNQPGGGIPGDGSSSVSYSHSATSSQQPGSSLQQQGSNNPTEPTPMMMESSAQAPSAAGSSNQASNPSAGGSKGADSGFMFGAPRAREHKNERKTRKTGAKPSKSQSSGSNASGAQPPPGGSEAISGSKASGSDAMVGGIGGSGIRALGSSNQDVTMMGSSAADPSLSSMDTIGMSTPPVGAAGTGSLSMGPSGGNTDFSSVNRRIGKVSRNPAPDGSGARPNSRVRSNTSSLNAASSSAAGGGAMEATGATFGSTGEPLVATGVPEVVPEVEGEGEGEGEAEDGEGGGNGSDSSRRSSSEHLSIMSPPEGSDVENVLMLICNIFQLPVALVAAYRSANIFIRSTALRLTDDDQWRYSLAQWSMLNAYELRDVLLVPNATLDERFREYYCVKNEPKVKFFMASPLRGSSGELLGTLCMADFQERNLEPTNLLVVNNLQTLVIRQLERDMALAQERVENEVAKVKLQQEMMRALDHFEKCIMFVDTSKPGWEVLYTNLAWEHVTGIPREHVLGSVMSDSFLDESRVPRVHVMGRPLQDYFMALGGAALDWSSTTDEVASRSEFTVHRVAVRQKPDKLVSLHLRLATEEATGELEGLLPKGLPGWVKARGNEAHNYFVFVEEVNPQAGTSSTVYEALAKHVEKVPGLELSHLLGKGGFGSVYYGTWNGMPVAVKMIDNKLTKTNTEGVSLEALMGQELAHPNIVKTIKYVMRTTLNASSVSGSTSRINSARPVGLHSRNRDGLGPYSGGIINSRHGSRNVLLPAVGSSGANPGANGANGGAAAHPTTGMMSYVTSAQPGLSGMTSAQMAAAADLTSASGMQHLNRMGTTMTSDATTVAGGGSAYSNQLPYGSSAVDASGGMLPREATLAAAAAAGATEAGQGSNHPGTTATSTTTSGLTAVGPASVQASSTSTAAGPPSVASAGGVPANQTCISTTASSRPQSATAPVADRLSSQRTALAAARARVSDSLSQSVRNDNSKPLPVEAALPEQLPPLVTPAAGTSSITAAYPPNAPPPDMQNLTAEQQQAAMTSRFSLAFSSITSGGDVLVPGKGSRSPSTSSDGKTQLTHNVNSVGSAEDMGEVWIIMEFCDRGSLQDALDRGALKLRKAGGEMGETHLPMMISTACEVASALHYLHDKGIVHGDLTAWNVMLCTAQDGQQDRSGRNFVAKVADFGLSRTLDVKSKIKTRTYGTISHMPPECLISGIISKATDVFAFGVLLWQMYTGLRPWAGMNHGQIIYNVGMKNVQLLFPPGTPPAIADLSSSCTRTNPAQRPSFNEILDKLDNIRASLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ10</accession>
    <name>A0ABQ5RZ10_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="210" mass="21765" checksum="904E138BAC7630EC" modified="2025-10-08" version="1" fragment="single">MASLFALAGGRNRVNTYSGLLNPRPIAVAMASGSQAQQAVPAVGALPDAVDQHTGRLDALANSGNETTLNCIPSMGIANEPVNVFAAAPPVPATQTPSRRTRDGQAILPIVEQSPSTTHSAAELPRDVVEYDAMEASDGEPPAKRVRAHAPQAAAAALSTGPSSASRPHQHDKRPSSGGRGNTRGDHRGHQPSRGGGIKPQSLMVDQERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZU3</accession>
    <name>A0ABQ5RZU3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="568"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="605"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="652" mass="69029" checksum="0FACD8E5655BA796" modified="2025-10-08" version="1" fragment="single">MCNGVIRVAEVEISRTLPQDLREDNDYLRRRVLLKAAQQAYHVQSTSSLMAPNIQIMEDNNCPTCNPSELAQTVTIRPKGTRCPANTGSPPLAVNYADISQPISAGNAPMAATNLLPCLFLDVDALLGRHVSSGHGTTVAALVKAPGTAPTAAVVNATAAAGAHLGRCQVMPVNVSDSDSLKLGNSLLNSGSPRWGRSLVDEDRENDIGAFNDCGSRVRGYANRADAGAAPRRQTLSGFDPVISDATGSTKISLTAAEAKTHTMSTAAAAPPPPTPRQCTVQRTEQNGVRALKRTIPGFTTRPGARRFLSAMKPYFALYAFSSSFNCNIDGSDKSADEWVDLALDLIDPLKTFPVHRCSQWPSATPTHGSGLQGCASQPCQARSLAQLVAAFPRVGVGAASADTVIVTCASRDLYGVLSDNVIQVVPYDSSSKRYDDVLDHIAAILVRHRAQLHCKQSNNSTCCHSPIQNNPGIGLKAGFKSANGKVRHVLEQLGLSPGPIEPSGVANQGLLSQSLAAASSATLAWCNLSRSSKRQGSNEGSCDVGRVVAATGAAATTAAAAAASSRRWQRVKRGSRSPPEARSWLSAIPEDSEGEDQLSERQESSPQWQQQKQIRPKRVPPSPTAAFNPSSNPMALRQLIVIPQALPLPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZZ7</accession>
    <name>A0ABQ5RZZ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dilute domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_005951</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF6">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dilute" evidence="3">
        <location>
            <begin position="3075"/>
            <end position="3319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="368"/>
            <end position="2319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2395"/>
            <end position="2440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2571"/>
            <end position="2591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2615"/>
            <end position="2681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="2">
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    <feature type="region of interest" description="Disordered" evidence="2">
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    <feature type="region of interest" description="Disordered" evidence="2">
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <dbReference type="SAM" id="Coils"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS51126"/>
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            <dbReference type="EMBL" id="GLI63181.1"/>
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    <sequence length="3765" mass="408254" checksum="74E5FAA7AF293832" modified="2025-10-08" version="1">MSRPQTTENESGGDQVPPEEDASLTSALDHLRRLVSDLENDVGRLSQALEAKELAAGKSKDVPELGAANTAAEALPCSTSAAEGRANTSGGWSAALSRTNSYRGTAVSAYRVLRSVLSLTPGYVGQTVGAIFKEARATPPLQPEQSPHPEVPQQPEQSLQPGQEQESHIVHTTAQVHTATMELSGAATPEIPSSLGLTTVEALGPTGVVAGPDINTVGARETTVVGRQCTASAAPAAGGRTAGAGAAVVLEVKATEAAAILKERGASTIAADPAIFEGRPAAAAFVATVSSTASPAKEAMGSCSKVVHTQAETKKATEGIAGRKITMVESAFTSATSREVSTMVQQVAAPQDHNSLELVDAAGNSHIDKHKEKQGTSASHPTLRKMAPTNYNADGEKTGRQDDTSKVTSAAATTKDKSKKQDEERLTKTDKTAMKEEEEQQPQKLEKEKANSAWAVPISLTKSAPGKNDADGEQTDEDEDSSMFTSAATGKKDKKSKKQEKKDKKEKERLEKMEKKMSKKASKWEEDDAEGEEDQQEVLELKEEEQQPKKRKGKADSASGVPNIEQQQEEQKLQKQEMGRPGGAASATAMIKEKQQDTKDEQQGEKQKQEKEEPPKKGEQQLQKQEKGRISTAQATPANEEKEKGKAASASADPIIQEKQQDKKEGLLKKEEQQPQKLEKEKADSAWAVPISMTKSAPSRDGADGEQTDEDEDSSMFTSAAAGKKDKKSKKQEKKDKKEKERLEKMEKKMSKKASKWEEDDAEEEQEINEQQEEKEELQKQEKGKAASASADPIIQEKQQDKKEGLLKKEEQQPQKLEKEKADSAWAVPISLTKSAPSRDGADGEQTDEDEDSSMFTSAATGKKDKKSKKQEKKDKKEKERLEKMEKKMSKKASKWEEDDAEEEQEINEQQEEKEDLQKQERGKAASTSADPIIQEKQQDKKEGLLKKEEQQPQKLEKEKADSAWAVPISLTKSAPSRDGADGEQTDEDEDSSMFTSAAAGKKDKKSKKQEKKDKKEKERLEKMEKKMSKKASKWEEDDAEEEQEINEQQEEKEELQKQEKGKAASASADPIIQEKQQDKKEGLLKKEEQQPQKLEKEKADSAWAVPISLTKSAPSRDGADGEQTDEDEDSSMFTSAATSKKDKKSKKQEKKDKKEKERLEKMEKKMSKKASKWEEDDAEGEEDQQEVLELKEEEQQPKKQKGKADSASGVPNIEQQQEEQKLQKQEMGRPGGAASATAMIKEKQQDTKDEQQGEKQKQEKEEPPKKGEQQLQKQEKGRISTAQATPANEEKEKGKAASASADPIIQEKQQDKKEGLLKKEEQQPQKLEKEKADSAWAVPISLTKSAPSRDGADGEQTDEDEDSSMFTSAAAGKKDKKSKKQEKKDKKEKERLEKMEKKMSKKASKWEEDDAEEEQEINEQQEEKEELQKQEKGKAGSTGATPCIEGKQQEKKEEVQKEEEQQPQKLEKEKADSACAVPISLTKSAPGKNDVDAGQTDEDEDNSMFTSAATGKKDKKSKKQEKKDKKEKERLEKMEKKMSKKASKWEEDDAEGEEDQQEVLELKEEEQQPKKRKGKADSASGVPNIEQQQEEQKLQKQEMGRPGGAASATAMIKEKQQDTKDEQQGEKQKQEKEEPPKKGEQQLQKQEKGRISTAQATPANEEKEKGKAASASADPIIQEKQQDKKEGLLKKEEQQPQKLEKEKADSAWAVPISMTKSAPSGDGADGEQTDEDEDSSMFTSAAAGKKDKKSKKQEKKDKKEKERLEKMEKKMSKKASKWEEDDAEEDQEINEQQEEKEELQKQEKGKAGSTGATPCIEGKQQEKKEEVQKEEEQQPQKLEKEKADSACAVPISLTKSAPGKNDVDAGQTDEDEDNSMFTSAATGKKDKKSKKQEKKDKKEKERLEKMEKKMSKKASKWEEDDAEGEEDQQEVLELKEEEQQPKKRKGKADSASGVPNIEQQQEEQKLQKQEMGRPGGAASATAMIKEKQQDTKDEQQGEKQKQEKEEPPKKGEQQLQKQEKGRISTAQATPANEEKEKGKAASASADPIIQEKQQDKKEGLLKKEEQQPQKLEKEKADSAWAVPISMTKSAPSRDGADGEQTDEDEDSSMFTSAAAGKKDKKSKKQEKKDKKEKERLEKMEKKMSKKASKWEEDDAEEEQEINEQQEEKEELQKQEKGKAGSTGATPCIEGKQQEKKEEVQKEEEQQPQKLEKEKADSAWAVPISLTKSAPGKNDADAGQTDEDEDNSMFTSAATGKKDKKSKKQEKKDKKEKERLEKMEKKMSKKASKWEEDDAEEGTSTPAMHAAQEKAVPATDQGDGKRIEEGEFMLDQSNINLNPPASAHTGRKLAAAIAPKTLSHSSVVVSTAPAEPVVQGTLISWDELLDLRETAYCGDSDSNYSVNGDTPPPAPGSSMAGCQSDHEERAKAWNKSPISENGSNDAAIADADGLGDRLLGAVVADLFNRSSAGDMTEDDANREGELLVSAASAFLNGGVATVSSENLPRRDGGRGSMCTSVGQLSYVTSSSLSGSDACPLSQPPLHRESCKSQPGKAVARLPCWKEDYRASDSAITVRRGHVKQPPPQPTGRGRSAVLADRHECLLAAVAEALPSISPVSSARSSSAISSDGEDDKRKISPRTHGAACPWSTTSAAVAVSSPSPPPIKAAPAPASGSTFSSPPSDSAAVSFSSQYSASLHVGTGAAGTHASHHTHQRSTGKPSDHLLLPPALTSPFSSPILLRPLVSPFADHPPFDDSSAASDIRTGGFSSGVESLPSLPSESLFRTACASPFGPTTSGSPSASTPVYGTARGTPLNDSGSMRVGESPLLTSGYLPWSGRFVYHPTASGNQYYATDGSVGLSDTETETSSDEDSSRTDDSSSFISTDGEGDSASDNEAVQAPQTVDERVIAAVSASRRCRTTKVVPAASVAAKPCRGWGEQAAACQQRGEQETQATRRVQACLGFRRGRPVAALVLFRYCLHMGALEDEPSNAQFFNRILVAWRKKMEVGKADVKCLAYWLSVSISLAGMLDTSRRIPPQVGKGSGDSGGSASAAAGGCGKGAVMGGGGNAVSGRDHNSSPANPVFLEQVQDFIRTVFKALYDNVQQQISPSIRGCVWGPTVTPPPSVMPLTASQGNYQLAHKGNRNGTPSASSYAGYGDDVASGSRPCSGAEASHQSWLDVLGVLDEVLWKMESNYTSPALIGALLRRVFRQLNVAMLNSLLSHAEACVLTNGLYLETGLRMVKIWLKARAGRSCGGRDAWEELQHIRQAVTFFLFPRRNTEGIIRPLLTVQQLLRFATSYKDDLYEEEQILPDVLKDLAAEVDGEPYEEQHLHPHPAGAAHSVPSVAADTQGGSSTKGKNGKKAAKDEKAAKDERKNAEGVVQDDSVLLPVLDVSTLSAGELRELINAEKFTAKDLYGSMSVPAALRDGHGGGPGAFAFLQVDLRARVLTTAASPPTRKAHKDDPVAIAPRPSSAAHATHLTIPSTADPVTSWPPRERTEQLGAHHRFQAALKGSGLAMVPAAIAAVASHRSSYLLTSGAAPAAAPAAAPAAAPAAQPPLPTPVSSPPPRFARPVTAGAQVYVRAPARPAAPVEPQGCAVGTTSRATVAASAAASAAAAEAARPSPQPVVALSPVRKSAASAVPVPQSRSSEVSLFYGQSLVSGPPVPPAPVALSLASLASDIMPPPASTAIKGVRMALGGGEWQVRGEQQEHSLGSRSRQRRTDSGSDGSSSAGSEDEPHFMLGSSKYTTEQLLRVFGSPLEMQDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0A6</accession>
    <name>A0ABQ5S0A6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arylamine N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053710">
        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF0">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="35022" checksum="1581762A58E3AED4" modified="2025-10-08" version="1">MSPSTCFDFNAYLQVIGVSPSQLIHGANPDSLKLIYSNHVRTLPFSSLPIVCGHPVDPDLDVAGIADRMLVKRWGGVCFEHVALLGAALQWLGFDVTYLGGEVWHDGWTAPNSHASILISFRDENRFYCDVGFPLRPLEPLQLSSGKEQLQPDGRCFQLESDPGDSNCWCLQQLEQGDFELRHRFWLQPRDLANFSGPARDLADPSSRFMRGWLLAICRAEGSLVTLATGLWAGSETPEDKAKYVCRKEVMGGASIGNGCGIVQPTQHIKHTTLLARGSSSLRSLVEREFLIKTKQLLACKDFGTEEGKEEDGDGAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0R5</accession>
    <name>A0ABQ5S0R5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anaphase-promoting complex subunit 4-like WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006423</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45282">
        <property type="entry name" value="OS03G0858400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45282:SF2">
        <property type="entry name" value="OS03G0858400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="120"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="319"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="6">
        <location>
            <begin position="370"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="51"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="461" mass="49910" checksum="5D67E820FA3F0189" modified="2025-10-08" version="1">MDAETAVPLSQNSQIVLAVAGGSVLLFIGYLIYGLANCLYSIASILGSTRNAKNRSKPSKQQKPAKAKGSDTDEPLVSAPTKERGPDLKKLIKAEKSAAKKKNTPKEDQMATHDLWVNTLKDHGDTVHGVAWSRDGQLLATACEDMILRLFDLSDVSNKNPKFKRVRTTRIPLGVGFGDSPDAVVATLRGTPDAMMALYQPIVKQPGEPPSYDAKWTVGNVHGKEPALLGGMASVPSDAAIGRRGFVVSLSTKKDGKVYDLQGREVAVFEPNALANHDLAVSSDGRFVAAATFSSDVKIWEMKYSREGEFRGLIKAMGLTGHRSQVACVALSFDNSRAATASKDGMLKIWNIDVRYELSEDPKVVLSIPMPLPDGRVYQHLAFGPPGSDLLAASYEGTVQLINLKNGELLERIQAHEAPITSIRWCPLPKKMAAEAGGAVAFVFATSSRDKRVRVWRAPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0U2</accession>
    <name>A0ABQ5S0U2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E1 ubiquitin-activating enzyme</fullName>
            <ecNumber evidence="4">6.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_006018</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.45" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01491">
        <property type="entry name" value="Ube1_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01490">
        <property type="entry name" value="Ube1_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.180">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12550">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2660">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.60">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, UFD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032420">
        <property type="entry name" value="E1_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032418">
        <property type="entry name" value="E1_FCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042302">
        <property type="entry name" value="E1_FCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038252">
        <property type="entry name" value="UBA_E1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042063">
        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018075">
        <property type="entry name" value="UBQ-activ_enz_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01408">
        <property type="entry name" value="Ube1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16191">
        <property type="entry name" value="E1_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16190">
        <property type="entry name" value="E1_FCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09358">
        <property type="entry name" value="E1_UFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="12">
        <location>
            <begin position="950"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="9">
        <location>
            <position position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI63113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1077" mass="116960" checksum="DB59EDC4D3278E32" modified="2025-10-08" version="1">MSKRAAEGLPDTPSAKKPTMSKSGAPDGGAGQVEIDENLHSRQLAVYGREAMKRMATSSVLISGANGLGVEIAKNVVLAGVRSVTVHDTVTVSLSDLSAQFYLCEQDQFGSPSRGFCVHLCRGHQDVGRNRAEACREKLQELNTSVAVHAAAGTLTDDFVKQFQVVVCTTATLAEAKRLDALCHGVGTAFIWAQTRGVFARVFTDFGPSFTVYDVNGEEPHSGIVASVSSGEPAIVTCVEDERLEFQDGELVSFSEVVGMEKLNTHGPFKVQNCKAHSFELAVDTSEWGEYVRGGIVVQVKEPKALVFKTLEKAISEPGEFLLTDFSKLDRAAQLHVGFQALDKFEAETGRSPRPADASDAAKLQSLAEQINASLPDGSAKLEAVDGGVLAKLAHCASAEINPMAAMFGGVVGQEVVKAVSGKFHPIFQWLYFDSVESLPDMEQLAAAGGVGADEYKPLGTRYDSQIAVFGRTMQRRLSALKLFLVGAGALGCEFLKNFACMGVASQLPGGAEFGVVTVTDDDVIEKSNLSRQFLFRDWDIGSSKSTVAAAAAQKLNPGFHVTPLQNRVSPDTENVFDDKFWQGLDLVVNALDNVNARLYVDSRCVYFCKPLLESGTLGPKCNTQMVIPLLTENYGASRDPPEKQAPMCTVHSFPHNIDHCLTWARSEFEGLLEKAPREAASFLGNPADYAKAVRANPDASARQQLEAVAEVLLDNRSTTFEQCITWARLRFQDYFHNRIAQLTYTFPEDATTSTGAPFWSAPKRFPRPLIFDSADSAHAAFVQAGAILRADVYGIPRPSWATDARKVAAVAAGVDVPAFVPRAGVQIETDPKADRTKSSQAGRTYDDEAVIESLLTKLDAAAQQLGTTLKLSPIQFEKDDDTNFHMDLIAGLANMRARNYSIPEVDKLKAKLIAGRIIPAIATATAVATGLVCLELYKAILPGKPLEAYRNTFANLALPLFAMAEPIPPKVTKHNDLTWSLWDRWTLEGDLTVQQVLDWLKGKDLNAYSISCGPALLYNNIFPRHADRLNKKMSELVVTVAKMELPSHRDHFDVVVACEDDAGEDLDVPLVSIKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0W5</accession>
    <name>A0ABQ5S0W5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="555"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="773"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="852"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="971"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
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        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
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        <location>
            <begin position="197"/>
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        </location>
    </feature>
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            <begin position="563"/>
            <end position="574"/>
        </location>
    </feature>
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            <begin position="652"/>
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        </location>
    </feature>
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            <begin position="671"/>
            <end position="680"/>
        </location>
    </feature>
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            <begin position="806"/>
            <end position="818"/>
        </location>
    </feature>
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            <begin position="855"/>
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            <begin position="872"/>
            <end position="882"/>
        </location>
    </feature>
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        <location>
            <begin position="890"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="926"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="994"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1031"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1031" mass="104485" checksum="DB346B1740C619E0" modified="2025-10-08" version="1" fragment="single">MKGYNGDHYHQTLSVYPRAEHTYRPRRSETSLEAARTSGDLDPARPALSAPPTRLISSATHQPSDHGNNVALLGSNRSSGMQLWPAQAHVPARPPATYGGATSDVPFPANAPVGDGNPTAAPIRGSGLEAVQMSLQDMLAAAARIQGELMASSSSDRPSTGPTAAAAAEAAEPAEAANAVVLSGALDGTPGMALTSTAPCDDASASTMAIPQPLPPHLAQAAPQSRGPLEALRDELFGSLSLTSPRLSGRPPMAPPASPTAQPAVLPASASASTTPTRMVRSAPGVVDTIAFSSPTTSVVAGTDEGGVSVVPLHEEISALTAQAGNSYADLSSIYSIPDSAINTTARTPMSFTTAAVSELDTPSSLRSRDDEYDKSFASPNSSERSPQGMGISAGRHRPSGRLEYDRITSGYDDYVSPASWMSLREQHSTSQAKGKSTVSCPGGGGGGGAMMNGNGDGSISSTPTSRPVPWTLDGGIELFASGTTPSPAMGAALPEASAAVAVAVAQSAASYDSSDVEGGQISASGRSEYAQIAASHRQAQNRIDVYWLQQRNQQQQEDGWVSSPDSGSSRSDPVMGAAMMAPPPPPSPEPEPEVATDASMLDIATQSLGQPHTTPTGFGSVLVTVVGADTGRGSEQNVNSDRMCRSLSFGVQSRSPSVVSSRTDSRYGDEGGGGGGGGAVPPPPAGVNSKYQASQRVVDMTTMGLRARETGAPLSLPMPYGRDAATAREVKGCGEEQQPKQHGDACYPFCNDTAHGHYDGATFAKPFFSPESTSGVGGSRTAPGHGGGSGSNSSSDGTSFVEGVSNGGGGSIIGSGSGTTTSLPTAREDVSLAADDGGPDKREDFHHDYRQQREEEEQAQEGAGQEEDNEGKEAQEERRMLEVMTDLLSAATAPGGTAPGSAGTPHHGHGYGYGYSNADAGSEGVLQQTSSSPRMASKSVEDANAGGGGGIRPSVSSSAVSVTDSVAGTENIQPLPIGRPPSLATALAPVSPPTTAGGAAAAAPSIDGMAAAGVLAAAPRPPLPRSGVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1F8</accession>
    <name>A0ABQ5S1F8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="284" mass="29629" checksum="C59B8FF8DF7918DA" modified="2025-10-08" version="1" fragment="single">AATTRAGGGSSPGGEAVVSGPGGSGPQTLSNLARAYVANHAGSTGGHGPNHIVAGIGTGAGARIVYPTAYDVAPRLAHDTHESTMLTSELKNIGSPAGAPGLWHFINAGTTLPHQRGPQQASSLLFLSSPSDTIGSFHGADSTTGGAGAATTAEVALPSGPIPGCTFEFDPYQSTADQATEAYDANHASIFTTCSPVHSTARHPSRLWGWALSSLRLVGGLDTSLVPPSQQHTQQAPQHHIQVQQQQYQMQQQQQQQQQQQQQQQQQQQQQQQLQQQVVPGGFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S266</accession>
    <name>A0ABQ5S266_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="652"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="829"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1136"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1231"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1351"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="445"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="484"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="829"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1136"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1195"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1409" mass="141017" checksum="39F9183FC671A34F" modified="2025-10-08" version="1" fragment="single">PRQVQFTSVGGAGSNSSGGGSVSVNGGGLIGSGSNISVGCFSGTSAGSNGRGSDWSAGGHRLQSSAGSVSCWAPSINTDSSGPGPSRRSRAVSSPSRLGLEAAVAVAAGSAAAAVTANNSGGGAGDGVWGTAPYGVPYPEAGPQYPSTYPAPYPTQYPGGGLAAPLPERADGNALPGNKRGSAAAAAEGQRRKGAKQELGLRWGGQTPSYTNLADAGAVSGGGGGGGAAAAGAELQGEGPSYLELAAQAAKLREKLSLMAVPRQSLSSQQQHLIGATGEMGPQSPTRTNGDATRSRSPHYDLIGKSKQEQNPPPQQQLWPGAPAAAVVATAGRATSYGGGEAVGVAAVPRAAMADRAGSAPVDWHAALQSKLLPYDVVRTRESLIDAAATLWAPAPPGVYAAPPPPQPGSGGGGVTRSGTTMRQSRGPSPSGIRPAMRRSSMGVGVAPAAGITASAAARSAPVTPRRANSPARSSLAAPPPQPQQQQQEQQQAPPYGGNAAVPSKSPRAGRAGSSGSILDRATPHPSGGGAAGNSGGGGVGGSRSGGSPQPMRPASPVPSEPITIVRPFAWQERETRLWLDELGLAVTSAEESLPLLDNPLRNGLLLAALAARVVGSPLPNGVVTDPPRDVRSARTNILCALDHLGLLAAPWGPPPDAERRARAQAATASEVGSGGSAGNTTAGGRSRSPSPARHAAKGPTTGSSGIIAIKALGSAGRGYALRLKERQLRAGLGLVAEVESVLAGSADSIWGLLNFIRLAVAPHYLHLRGTGNGTCAASTAAAAGRRPWGNAVALSDEPVRRTFDVRGGVGPDGTTGLGPAPAVVAAAAAAANDPPTSRSGRASPGRAAGATADEGGSQPAGVTATAPSPCLRGGGKVAVVGGGRNRVTGGPVADPRGSSHLAANGNGHALGTLSSATGGAVTSPAATENGLLYRPLWPTLAALPYGPAEVTALESSLLQWLQEAGAISRREAAQGFSAMLPMFEDGTFICWLVSNLGRKPIVGAHRRPLTEAARRTNWLKAIEAMRTIPGMSRRFLAYEEALIHCERPLLTGLLEDLHRLAGGMPPAPSKILPDAKPYLPYTVPEPVVRTLPPEHIAVNCGSVVSSPTRTPTPRRASTGVLPMAMAMGGSCAAMKSIRSTPSASPPTGTSSCQRQGSSGAAASGALTPPPQHQQHPLFQKAAPLSSGDISSAIEGKDKEETRRDGADADAAGPATIDIAVSAADGIGGGSGTWAGGSEPNFPREAYNNSPCRTNNKRGGRNAAAAVIAASARCAAAAAAAASAAGAGSAGGAGRGLITDIMVTPSKVLPPDQQQHGMRRPVSASVIAADLLHGSYPVAAWGHDGVDVRQSVPGGTTGNNDHNYPYHHQKGKALRSSSVRGSRSVGGALEACSSSPAISPRLRLVPSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S286</accession>
    <name>A0ABQ5S286_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="179" mass="19339" checksum="C8E852819F249B7C" modified="2025-10-08" version="1" fragment="single">ASAADSPACVYGCDASYRIRLASYDGAQTLVSVRVVQVSLRYMLLYISYRYYQLDAITTISELLYVPIVAPWTQPAVSLYGSPTADIGDADSVSSWPPAATDPRDTAWGLQLLGDGGGSRRLGEQPAATWSAEQLPYTACSRQFSLTIIFKMRPPVARIGRWHLATMQLSPDAAGGGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2M5</accession>
    <name>A0ABQ5S2M5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="550"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="771" mass="82684" checksum="E248FC8EE6EC4A8D" modified="2025-10-08" version="1" fragment="single">MSSLHWALPVNQPTETVFSPAFPSIDASLAGTCGGLLGIRPLLQLPASRRLSLEDKSTEAQVIRKSVSFSLRARGPGAGYNSASPVQQSPPSQRPDDHPEQLNSSGGGLGEVNEDVVRRLFRAVQGHANSPEPQPKAYPGWVPQPQQDKPKTASAEPLPAQNPTPAVNSIPALDPTQSNGINLTCKGPGPLLRRRDEAGPVSPQLEGRNLARMRPAISCASSVWYPLTPCPSGGAGFAAADMDNEGCASIRGGEVHSHFSTSAGPRHPLAIRQQQQPTGPQHEERHGTRSQQHLRTHVTGAELPLPASANTSAVQRPASAAASWDRRQVMPTQAASKRPSPSAMPAPASSAPVWGFVKPSSAGIRLEPPIEKPRPCVTRTFPMAARATSALPMMTETAVGITAAATVERSPVMSHRPTQSPAANSNACPTSIGGSQDATFEQVHMLLASLNLPGIKAGLALGSDSPQCVGLISEAHEHQSGDLAITNTFMDRVTELTPSGMAIQHPPGHGGQREVWAGSPTANGRAAEDIGSMSGESRHLEAVVRSVDVPRGHQPPMEDARIGVSQGPKMDNSRWPFDRMLAARGSLGGEWFIEALRPACCAWLQSVARIAGQQRYAELHDMRKLIQQIQEARLERRHVEAYLMGNRLGIPRARLARLHELAVASGASPAVTSTLLSQLEDRLRLKLGLKALRQNAVVRTRLLELLQSRHETGGPLAVAALQCWRRAAAGRLIMRGRVDTMITTRRRLNARRVLSAWRATAHRSGELRQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3Z7</accession>
    <name>A0ABQ5S3Z7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YchJ-like middle NTF2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007421</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048469">
        <property type="entry name" value="YchJ-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33747:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE-ASSOCIATED CAP C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33747">
        <property type="entry name" value="UPF0225 PROTEIN SCO1677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17775">
        <property type="entry name" value="YchJ_M-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YchJ-like middle NTF2-like" evidence="2">
        <location>
            <begin position="96"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="239" mass="26274" checksum="5374827B18190EB8" modified="2025-10-08" version="1">MINKHSFRVFGGGINSKAPRRQRKATDPAMSSPRHVHTVVVYAAKGSKSTATKGFGAPKPQSSKGIAADARCPCGSGLLFEACCSPYHQGQAPTSSAEALLRSRYSAYVAKEPSFIADTTHPDSPEYTGSRASYISTVKQTMRRLDPMQLTILTSEPGETADEWFITFRLKRRIKDPEVKGSAPEVDELTERSRFIRAKGRWMYLDSRFIEEEGQPQKAESGAAPTPEAPAKKGFLNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4D8</accession>
    <name>A0ABQ5S4D8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="1">
        <location>
            <begin position="158"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="325" mass="35188" checksum="2C10638184FF598E" modified="2025-10-08" version="1" fragment="single">PPPPPPPPPPPSPTVLPATLPSTPCEVCIFAELLPPAIDVRPFRYDADTCAYFQKNISATLNGFIVSKSMAMYTYFEPDPDRCSELIVSTCATFSSMLDAQQLSNLAKAMLAQWTAAAAGGSVCRPELEGYEVSVYTSDDSCLFFTSSVGCHLPAAVFPNRTRCNATQGTLPVVLDPRYYLRPELLPDQPTSSFCFMFGAIPQDQIVPSTCGVANDKLTKVEWYANQTMGSWIKSISLHPHAGSMLLIQPSWAETGNTLRAPSINWTVDQANGAMICINVIKPKTLDDICLGGLPSQCYGSLFGTSNTCCPVYRTGLTRMFAGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4E6</accession>
    <name>A0ABQ5S4E6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="120" mass="13154" checksum="FAEA80009609DB65" modified="2025-10-08" version="1" fragment="single">DTDAVYLSEPAAEVQKAAAVALFEQHDPAYQALVVKLLVHVSVNLGHKMRPWALCRLMSEAAMEDDGKEEDDLRFSLLPEKATRNRATGGAGSCLRQKWPEVLGGHQVENLPGDTGICLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4U3</accession>
    <name>A0ABQ5S4U3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Coatomer subunit zeta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_007748</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPI-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14829">
        <property type="entry name" value="Zeta-COP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039652">
        <property type="entry name" value="Coatomer_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043:SF0">
        <property type="entry name" value="COATOMER SUBUNIT ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043">
        <property type="entry name" value="ZETA-COAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="13">
        <location>
            <begin position="11"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI64474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="184" mass="20560" checksum="11C93AA829894EB8" modified="2025-10-08" version="1">MATMDPTLPLVKNLLLLDSEGKRIAVKYYTPEWSTVASQASFEKALWNKTSRTNARAEAEIIMFDNVVVVYKYVGDLMFYVTGNQDENELVLYTVLQAFYESVTILLRQQVEKKTVLENLDLVLLAIDEIIDGGIILETEPSTIASRVTMRGADGEQAPVPITEQTFSKALASAKEHLARSLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S568</accession>
    <name>A0ABQ5S568_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dehydrogenase E1 component domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007863</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="2">
        <location>
            <begin position="149"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="2">
        <location>
            <begin position="388"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="512" mass="55467" checksum="574907A21A3056A2" modified="2025-10-08" version="1">MRSRGSSAMLAHVVKTIGAFGQVKDTSSATALAFSISCDRIDTSIRFYPCSIGPELWGMSLRRHLHFCSSQGQAAVSVGSPSSSTTDNFLEVPGGRLPYTSQLQFVGGSETPAPTIPCYRTIDSTGQDVPDAHVPYPISQELSLRMYTCMARLQTMDTMFYEAQRQGRFSFYLTCQGEEATNIGSAAGLDNKDMVFAQYREQGVLLWRGYTLEQFANQLLGNAKEPGKGRQMPIHYGSPELAYQTISSPLATQMPHAVGAAYAYKMDRISQVSVVYFGDGASSEGDAHAAFNFAAVLGAPVIFICRNNGYAISTPAHEQYKGDGIAGRGPTYGIPTLRVDGGDVRAVYNAVVAARARALGMPYNSGGVTSAPGTGGGGLGGGSETSLGPGPVLLECMSYRSGHHSTSDDSTRYRTSEEMRAWRARDPVARFRTWLVRRGWWDEAQEAALRRTTRVQVLEALDAAAQVPKPNLSTLFTDVYDTMPPHLEAQRAQALEFVRRNPSVCPSDVPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5U8</accession>
    <name>A0ABQ5S5U8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-tyrosine-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_008775</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46377">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46377:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="18"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="76"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26528" checksum="444FD567DBC457C8" modified="2025-10-08" version="1">MAGTDVFHNLSTAVQLNNPQLIIPGLLISSFQFETIELQLREQGVTHIVQVGIELRQSHKGKFEYLHVPIQDLEGVDLVEHLPRVFEFMDSAIAKGGVVLVHCMMGISRSASTCIAFLMWKHRLSFVQAAEQVYAARPFISPNPGFVLQLMLWERLGMDFALWEGWSYHKFSGAKEQLGGLERCMFDTVMRDAGVNLANVSEQRNLGNSVFLVPSPPPKRRPMPVKWTQRFCCIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5W6</accession>
    <name>A0ABQ5S5W6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008378</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="22"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="303"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="446"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="682"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="744"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="303"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="514"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1153" mass="123138" checksum="B035C2DDE35F74F6" modified="2025-10-08" version="1" fragment="single">MALTPVLSSAPNFSIDQGLEYYAGKTELLDWINHLLELNLTRLEQLTSGAVYCQIIDAFYADIVAIRKVNFYAREEYEILPNYKVLQAAFNALGLTKEPNVAKLSQASPPDLMEFAQWLYKSFKGVSTLSGYNPSLRRQLSVKGGTINVPLPACLVNTTFEFSRIDLRTSGNWGATTPQTQESNRGAGRANRSLGSTPGTGNKSSAGKRTPPYLNVASRYKDGLAVPPDHFERRSPRLSPGSTGGSQRKRVMVSHGSTQTDFPEEDYRPMTAADVGTEVSWPRAEQRDYGQQCEDMLVYTGSLSPSGSTASPSASAPTPHALGSETFSFGRSRSGIPSAPGIHFSPRTTSSPTTSVQLEEREPATVGASFEDDGRYGDNGSPATYKQMLQTPEMNGEVTAAIFCGKALDREDASACGAPEQVAEEGILNTFRHAAAAASFAETADTHGAHGEALPGAASASACAKENDEQDERSPDATGQTGSGEGAAEEDDLGAAGGDPSCSPSALPPRERLASPGVASSPAAASVAASPSPRAGQRWRNSPTVLPADVASLLASPVARTRLASPACLRDMAAALDVCAAALQAVDKALGSYDTLLTEAVHSPQPSDPSKPASQDAASALSCSPALCGKVRSLQDALQTLLEHTVLLLSDVQAYSQATHTTEQHHQPRSRTASPSAALEMVASPGRSAPAGTVPPGSPGTGNTQATRNFLAEAAVLLQRAHFHEDRLRTLSSRTEEVLKMPVEEPGATGGVGSPHPDRGTPASSGTAPQRHLTDEQRKLCSTAQLDSHLKAVGIAQFALVQDIHKELAALTATYFRHTGITIGGDGGSTGGGDGSTGGVSTGSSRKARVFKLGEYQLKQLRNGDIYKGRYVCNKKNGEGVYHFINADVFEGEFRDDRMDGHGVYTFRHEGRYEGQWRNAVYNGTGAETFAKGSTYHGEYTGGLRNGWGVCRFYNGDYYEGHWGKGLRDGSGMQQCTDDSNFVGDYQRGKRHGHGVYSFPNGDRYEGQYFEDLPHGHGTYHFASGQCYQGQWQQGKKHGWSVYTVDNGNRFMGLWSEGKPIWVQPLNGDAAAKASEDNSRSLQQALEARDLAVQAAVDARKRAASHWDMASPIQTRLRSVLSQAQASASLAQQARRKACDLAARLDAAVTLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5Y0</accession>
    <name>A0ABQ5S5Y0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_008852</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="379"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="199"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="242"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="497" mass="53207" checksum="AF2D0EEA768DF424" modified="2025-10-08" version="1">MPRCTGTNDERLDIISDGHQRPLQDLRLGCHAMAHRAERLSAPLATSLHPSSPSVSPKLTYQSAQSSPCLSSPRPFTASKHGLPVTRDTLLTEPYRLAIEPDLKYRAEILHPQFPGSVLQPACSIHEATSCKKSETAHGPQSPYRVIPLSGPTASTNPQSSPSLDLSWQNLAAMYISALAGHTGSSPAFASGSGSGFGFGSQSSFEREADTVPVPAATSPWSNRDPAWSPDLRHQDLQQQAFCPSGIPGEGPGHEHEGVSGPRPRARQSCQPEEGNSGGRSGSAVLKTFLQCPSRGGRWRGRPFDALRLPPPRLLHATQTRQKVEGRGPGWGRRVGIRAVPAARELEELVEDEEVAGEEGMATAAAGRDAAAVSSPPHPQQFITIVAGALSRTAAQVAVHPLDTLKTRMQVQLRNPQLGVWRAVMSCPGTRAQALAAWTGAAGARDLLLGLGAAVAGILPASAVYFTAEPRIRSWMAESIANGKWVRFRSFRFDRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S608</accession>
    <name>A0ABQ5S608_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI65015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="643" mass="66753" checksum="1196FF07BB4DDEE9" modified="2025-10-08" version="1">MPNVEEEFGKLNDDITRFLKEEGCVDSGDDDIEPYMAPRARPGSITPLGVRVDRGAGLLGFNSATSTIGEMDSVLTNIRAWKQKHEREMLELRQRNEHALSQVAEAVTQLEQASPLSSNTSREAVGTDTSAVATPDLWASLLSPSRQRDFNHAKVRHEEQQCQQQQKEPASGKEQASGLPPPVQLGEEDDEDLTTLLRERQERRDRILSGSATAKLGLTSKTAQAHIPVQGQAFGYPIADADAQSQQSTATRGGSGGGSSQSMSTTDQLPLVSVLRASMPCPARSQHLLGSAPSERPTTAARRRAEEAASAAVFRGQQLMSELEAELRELEGGERGRSRTDLRVSFTDRGSDVMGVGAGTATSVGLWEPGSPRSGTSMTFTANGVLGDGGNDEDADHLASSATVATAGYPHVEFLLDWSAQLQGVLAKMDALQTGYQAVAAAAATATRRPGSGNAASAGLGARVNSPGSGGTCASTAAVAGWGTGNPSGASMTSRPGSARLRLEPLPRGPCLLSSGLTGQEAATGLARIPSPSLPSSSVSAMADPALAVVAGLEGSGQGRRSRSRVRGLRGTAEGGEVCAPALKQGSVTDNRDDTRNSSCEREAAESGAGPMACAGHRIRQLRELQRQGAGGTITGGATGGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6F2</accession>
    <name>A0ABQ5S6F2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="377"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="403"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="440" mass="47583" checksum="A646C50116D58809" modified="2025-10-08" version="1">MEELERCLASLDGSPSAEVLARARSAAETIKQELEMHKEAAATRQKTGQDWQTIASERETETVRLRAEINRMRQAIASSTQAHGSQAEEIASLQAQLQEALAINKAQQRQAVVTTSAVDGPNAELYRLRAELEGRTGQLQQMEADLANQRAVAATRLNEMQDTFSNKITEMRRMHQQQLEQAVAAARAAAASEAQGRTGPRAGAAALEQQNAQRLQRAEADVASLRQQLEAAQAASTELQGKLKQAQSDAESSRSQLTSAQFGEKALKARVKELEGQLAKTQQQVKEQQKNESQPPPPPPPPPPAKEAPAVDLSMLNMMEGQLGRLSEIIRSREGELAQMRAALQAGLDERKELLQQIEMLQTALLQAQQQLQLQQQQQQHYGSAPGSSNGSINGGGGGGSVSGLSSPKSSSGAVPRGLPLVGQHKPNRTGLQSKPRFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6K7</accession>
    <name>A0ABQ5S6K7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="135" mass="13313" checksum="627BA3585A8002E0" modified="2025-10-08" version="1" fragment="single">MTPEQYFSQHSGLMSGGNTPLGAVHPGSMPRQISAQGAPGGPDIVGMLSQMQQDLASVNARLNSQGPGGASVRHVGGALLDGTTMVAAADGATGGSGLPAFQYGAVRSGRGRVEPLPTPPSVAGGGSTSAMVDWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7Z5</accession>
    <name>A0ABQ5S7Z5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LamG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="36003" checksum="5EB621CEFB1B7186" modified="2025-10-08" version="1" fragment="single">PPPPPPPPPPPPPLPPPSPPSPPPRPPSPLPPPPPRPPAPPSPPPPRPPLSPPAPPPPSPPRLPPPPSMPPAPPAPSAPPSYDTKTIMFVGPAASSCPAAGTVTVVQLGNTSTALAAEVFSAGGVSNGTLAPTLVGGLVYLATLASNTSLPDQPWAYNGLNNQSCALLLNHTSCGSCAKVTYNVPAALLAPIKASRAMSIEAKLWLTKWVNYGIDNADPMFGFQQAWDRYLKLVSDKWQMGRALFMAPAADTTAQGLFSGSFVNRTHLEAALTLGSWHHVALSINETSCALYVDAALVATPVKCNVSALVKYDSAATITVGGFQGYVSGLRFSSTWRTTVKPVPLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S892</accession>
    <name>A0ABQ5S892_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009319</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="217" mass="22702" checksum="A1A4A1F7A28E6B41" modified="2025-10-08" version="1" fragment="single">MNKHLCKHPFAAARPRVRCSATATTTTTATPGKVVDLKLWKPINLTKNNLIGAQGGETTRNVDGVVYFVSYDASKDEIVVVDRKLGVRFPAAADDQNRVRIDAASPLPHAVAGFNVEAYQPVLRADSPNAETINGRVAMVGFLGVAATEIITGQSALQQLASTSGAAAAAVAGLLTLAASIAPAVMGRVPAAKVVANEADSFPDRPLPYTWNALAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8A4</accession>
    <name>A0ABQ5S8A4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_009860</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="958"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="320"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="433"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="667"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="795"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="843"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1008"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="436"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="674"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="689"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="711"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="813"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="900"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1042"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="621"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI66130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1324" mass="136921" checksum="4C48698EE9C4D73F" modified="2025-10-08" version="1">MVGPAVNRRFPLRSVHAAFTMVSVANSLPSTSHNCISTNYQLHRPSRSQATLFLLVLAVVIGCVPFPPASVASASAPAVTAAAVAALMKVSTATQLVDAILDNGIPSVQLIANVRVDPSVWLATDPIIRFTNFTIVGADWYPILDLNFAENKIHLQPGVYFVFGQVELRNVRTRSGFELDIFDRSPNATVRYQQTVYFCFSCLPPSALVSAIYKYPPASLPNDVRNASWQRTQDPLQQSYGTGQAVLFATASTVMPTTGVLGATSGGGYELQAVDTLFVCAEPIDPAECESARGMDHCLSRKIEAMAAVDDTWRKLEEAAAERATATEAKAASGKRGGSSEDNARNSGAGITGSGGSRHLPVAAAVGGALGGTALLAAVAAAVVLHRRRCCHRRIDGAMANLTSVGGNGAPPPFEDSMAVKIVMVEEIGNAGDGNGDSRCKALPRSRDQQPSANGVAGNGHDQEAVPVVSSAAAAAAAAAAATAAAAAAATAAATATAPQSSHPHSPSHRYEGDAADSVVSTLDLQGTYPEVMAALADGGSLSISTLGESYQVPSSSESPSQWPRFMSMAPAMSSKSAGEERSQPVVVVAGITIELTNVIGTGSFGKVYKGSWQGRPVAVKVLQYGAELCRSVHSEVLLSQTLRHPNVVAALYFAQVLPGGKLGLQTSGGAGGATTSTDSSGAGPTPPPDRRSSDRTMVMEKPEAGDHVRTSGTGTGASPAGSYGVQGKRLNGCDGCGGGQEDAAAGRYNRSISLDLVQGSETMAQTAPAEALTGGSESGVVDFKAMSLPPNVCRSSGTSCGPDGGRDGGKQVGSNQGLPAPLSSQEAGNATAHLAGLAAAAADPTAGTAAETLGPRRDGRPTSTSQPGSERLELRGRAQPDNAPLMPVRLSELGKQEVTELSESAPDDTTGSSHAGDADTDATLAAAAAVTTAGDVAERCRSPLDVLSRWDEQRRRQEYVERTGAKLIGPEVVVSSNGFLMQAGVADSGGNACKTVVALMARDKKAPVTCEDGGGVGRDGSEASAGPRAKFASDGTGTTHSPSARCSSPTTPSPPSQPRHPLTTGPSTPADAAAVALPLEALWLAAYTQPAAGGSHGFLVMELCAGGSASAWRAASWSGPDERPDLVMCESERARARESVLLRLALDIAYGMSYIHSMGICHGDLKLSNVLLARSSDASALSRANPNDMLAGWVAKVCDFGLSRVLTGGRTHVSTRPYGTPTHMAPELWTKGHMSQPADVYAFGITLWELATGERPYKGLNAARILHRVLLNGDRPIMPLWLSPSYTRLVTSCWAQSPKDRPTFGEIVRHLEAMLAVLAAVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8H1</accession>
    <name>A0ABQ5S8H1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009811</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11030">
        <property type="entry name" value="CYP105-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002397">
        <property type="entry name" value="Cyt_P450_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46696">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46696:SF6">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00359">
        <property type="entry name" value="BP450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="48455" checksum="3F91BB43B5458380" modified="2025-10-08" version="1">MAALSMARVLKHLSAAPTLGASRSVRSTSAGSRQLVMTRFVSDNTSFPFSRPSGGDPPTEYAELRKTCPIAKVKLWDGSKVWLVTKHKDVCDALTNDSFSKVRTHPNFPELSPGGKAAAASGRAPTFVDMDPPQHTKYRDMVAHAFSREAVEVMRPGIQRKTNELVDEMLRKGSPLDLHEEFAMKLPFKVIYEMLGIPAADHAWLSSNVAVRSSGSSTAKDAAAAAEDLVHYMDRLVRSKMQNPGDDLISELVNKQLRPGHISHEELVQTAFLLLVAGNATVATQIDLGVMTLLAHQDQLAEMKADPERLVPLAVEEICRYHTGSSFALRRVAKQDTRIDGQDIKAGEGIIALNQSANRDETVFPDPDRFDIHRNPNPELGFGYGTHVCIAEWLARAEMQEAFAALFRRMPNLRLAVPESQIRFSDPTRDVGVAALPVTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8Y2</accession>
    <name>A0ABQ5S8Y2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="512"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="700"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="749"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="908"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="972"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1161"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1242"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1354"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1532"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1802"/>
            <end position="1825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="645"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="714"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="749"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="911"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="972"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1249"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1395"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1536"/>
            <end position="1546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1554"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1825" mass="183753" checksum="C1D3173BD0CEAD45" modified="2025-10-08" version="1" fragment="single">GPRIGGGVNDNPEDIYNSDIVHQSSRITGQKTILGSSADAGFASGSGGGSEHVLMHPVAVKGSTPEYAGCIGGLVRGGNSSGDGGGLAAGTRGINVQANVGVEGDSTSATRDRSSEPATSQNELLTAAGARTDSTLNTGNDPTQAVKEGGFGPDGCHELTSASAGTARGPAFTDDDAASASASASASASGSAAVPYNGCFGPSGRMMMKASASGSEAGEVRATWPCQHSPGKGAAESAVVAAAAATAMKIGALGLAFPQPLPVVQEVHESGSFSHDAASREASIKGRDDGQGNWINGNNGGFRALGDDSETDKAGTGNKPGGVGSSYNTDPLEEFSYGVHSCHPDLRSVPLPQLQLPENGSGASVCCSGRAGDISRRDSIGQTQLLQHLPVAPNPALYVQQSSTEAAVRDGQIPYDRPSALYNSTPLPVFNTYGPRMTHGSLGATMESDLERIASWVHVGNSNSSNPTSPTELGTIPDPKQLATVFSTTAAIDMQIRTVVGPQLNSIELASQHPSLSNSNSNSNSPRQSDGVAAANGQPMGPHSIGRSSRFFSMLDRATPPVRRYPFTNFSSSQQMQNEGTPVARDEGTDTDAGAMGVMQAQIAALRAHLLNPNANPFPDILGGGGGAGVGGAGGGLLGSTPSHHLPKHGRSSGRHGRYQLPNVRAAIVRVRDAAAAAASGTTNRQEVAAAAATAAATGGAFGTAPRTSPPPATDSSSTTAPAAKGNLSPRSVAASVVAHMSRRHLSGGNTAFQESAPPQTLQDDPSATAAADATARVTPATAATAATAATAAPCVFFTEPQSLMNVAKTVRYGNGIGRPYSAGAQPALALGSTAFSSMGSKGSQPVLLRHSVTLQASGDGGGSGGSLHQQYCRHHEWSQHQHLYYHPQDQHPQQPQSPAVLEPIGEVKQAGDGSSDGISSTGGSATAGQQSLAASGPNTSPPHLTPNRNPVAPSTAASVLSFLKTALLSSNRSASTSDFRQTTTGGASGGGTTAERTDEKRFLTASSPSGGGGAAAAVPNFSAASSPPEPLQGHAVATPPTIAARRISIPQTSSLGELCTTTSLTTTPVTPVSITAAATAAGVVSGAATAAATAAAGSTGNTAQIYGLTETSTSTFGNAALVSPSTATAAADYFTLQHQHNFEIRGTVITLPVTLTPPGVFGRRQKGSALPPSRRTSMDGGDRGAHCLHTTASTYDGRLSLPLAKLRSCGNELDAQGRHSNNNMNNNYHFYYCNPLPRESAADAGRQPDGGGGGGGRNLENVYISSSGGGGGGGDPARRIVSHQSWGHARHNAQQHLAALSSGAGRHPTRTNTGTTTGTVASASFAEHPAAAAAAALPIAAAGKAAKVPPPLLFSGSPPNSSSCRGGGCDAEVGSNSSPGGLWRRSRLSNCGTAGFGASGGPSPGAGTPASSTAGGGIMRGFRSRRANAFASGGGGGVWRGGQGDSNEHLGNLLGRAADAAAVPYSKSSQLEKVALHRSVLHGGSGSGGGGGEFDFDGGLRTADRLGGGGSTARDAIMGLSSMLGVQVGYCDGSNDGDSDSDGDGDNAYISDRGGGGSGGGGGGDSGGDAGGDSGQSALEMLDESNITLAGIICARAGISAATADIAEPLTMMSPAVPLPLAAPPQLRASTPSTDRTTPGGGFTCDNTASATASDAATIGFPFQQRHWQQQQQQAYATNFDYMAPGVSAPLPGKMNAELHPSPYTSSAGVQQLATNSARLPVPLVRSSLKGSGRLLPVMPEHLAEAEVEARTAAMALPGRRSPAWVIRRINEAKQEKLVSEVSAWLRAALEQRGSRRNLEDITWNPASTEAEGSSGPGRTAGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9T0</accession>
    <name>A0ABQ5S9T0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="223" mass="23600" checksum="615C9B1D00C195A2" modified="2025-10-08" version="1" fragment="single">LPATNFRSQRELSSRPAGPTARTQKGEDPSEAQIDALRKQLSEIQASLAVLSRKETDRSNSYPVSSSAGYALSPSISSTAKQVLHHLKVREQDLRLGIAMALQELNLERPDGGKRLPEAEQHYRAVIASLEAMAASHQMTDAVASPGASSGDGSPLGPASPPPVHSRGGSSSRRPLDGDVDPQITRLSVIGNLAALLLEADRPHEALRELDRVLEEGQGPGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAB1</accession>
    <name>A0ABQ5SAB1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Uroporphyrinogen-III synthase</fullName>
            <ecNumber evidence="3 7">4.2.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_010844</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>hydroxymethylbilane = uroporphyrinogen III + H2O</text>
            <dbReference type="Rhea" id="RHEA:18965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="EC" id="4.2.1.75"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the uroporphyrinogen-III synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.75" evidence="3 7"/>
    <dbReference type="EMBL" id="BSDZ01000044">
        <property type="protein sequence ID" value="GLI66857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38042">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38042:SF1">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="8">
        <location>
            <begin position="52"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="287" mass="30304" checksum="CAF50788EE36D4BD" modified="2025-10-08" version="1">MALQSYRQSTWSGAALARRQTACRSRRVVVFASGAPARRKVLLTRESGKNEKMRDALSRKGYTCLEMPLIEHTDGPDRALLPSLLSEAAEQYDWVTITSPEAAAVFLEGWEQAGKPKVRAAVVGGGTGDVLLQGGITPDYVPSKALGKVMGSELPRIPGGTGRVLYPASVKASTDLQDSLAAAGFEVHRINTYNTGPVRSVPESLLREALTADVVTYGSPSAVKAWVGLAGLEVANSKVNACIGSTSAKACASAGITENVYFPDSPGIEGWVDAVDRGCRDLQLAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAC2</accession>
    <name>A0ABQ5SAC2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000044">
        <property type="protein sequence ID" value="GLI66867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="191" mass="21277" checksum="196A055B495DAA55" modified="2025-10-08" version="1" fragment="single">LMEMVDADYEMPPTGALGQSVAPALSANNFHDLMEEPAVSSLQLMASNTPSAKMSREKLPDNTHENCKDRGRESRKEMQQLHSQHHQLQQAEGKGPGAGGVEAAFTGLQHNDQQTELEQLCARRTMLRNATELAVMRMVSHVCIVQAFAVYDNVIMKRPPGAVDSFVLRRLDPKNPDRRVPHLHRHRPGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAC7</accession>
    <name>A0ABQ5SAC7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010884</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000045">
        <property type="protein sequence ID" value="GLI66888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046771080" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="42"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="280" mass="28792" checksum="767E17571F0F0AC2" modified="2025-10-08" version="1" fragment="single">MARMTWLLVAALAVAGAAGAYAQYYDDDFLAVRDQTGLIPNFPFRDCSTRIGPYRLAPDVKQYGGGKYCFTIKVQTQNCSNACCQADLFKIEFNVSDSCMGILPAPEIKATINGVPTRVGAGLDRPVNGRNGTAVLRLTQLGLTTTTAADAELCLTLKANRAGRGCTTLEQLCATQNGVPRGTCTAALYDSPCVCCPVSSAFGPSPPPPAPPSPAPPSPDPPSPAPPSPLPPSPAPPSPLPPSPSPPSPLPPSPPPPSPPPSPPPPTPPPPPPPPPPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAI0</accession>
    <name>A0ABQ5SAI0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="501"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="646"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="727"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1002"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1076"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1199"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="646"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="692"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="727"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="787"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1413" mass="150695" checksum="81758EB42CDAF242" modified="2025-10-08" version="1">MIPQPDQQKLASLEEEISSDILLPEDLIDLLDDAGIEVDDDGLLSDNPTLTSNPRELPDAAAFPNDTLNLTTTAKCDGGSAAAQQHPDALGDLLSRDLDSDTIGFGHHRRLSSPYDQPSAPRECRFPQAYFPLRPPHESAAACSSRSTATLPQQAQQQEQPLDQLRWQQPAAAPSDDHLMAPTLIMLQQQNSQLVPPSSNPAFGDAIDDGPNLSMSFCHPGSGGSGGVRLENTSPIKAPVMQSADAAGTSGVQHVDVLHLFSQPMQQPMQQLQSSPFKLPQPPQPQQPQPPPPPPPQQQPPVGQSWRTAFHHLPAEQPYFGGSGQKLQHGPSTSTSYIWTADVPLPAAQGLQFLPWMPFKAVPRDFNNRSGDIGGGSVVSQPITPPCIDDILECWWRELGGVSDWAAGKVVWMEQQKLGERQHEELAFISDKCCWARSQIQFPGTLVEKLWPNGAGAACTASKGLGGGGGSNQGAHSGLKCQTLQFVGPSELRMFGERQGISSNSGAKQRFARQLKQETATQQQQQERQEQGGGGTTVGLYVIDKYTYTCSGKPNSSPQVEKVMLAQYVGDANQEDLKELNARNVKLKGRLTNSDEWHSGIFGLKGSTPSSYVRLRWKAWWPAVYTVKAPSGRSKAAKATATAGTSAAAASGGPGPSMSGPSRQVTTCVIPIPGSPSTAALCGQGCPASPPESMSQRGLAPQTTRHLASLPPQPCAIVQIAPLQHVHGQHAQHQHRQGFGHGLPQQPSHGPVGQGAASMFLLPHSSVSEQWGQPSPFPESTRRDDQVTTGPTVGLGPTPSSQDMCMGSITAAHYQTALPGPQAVWFPGPVQGCSGLPVQGGLDLAEQALWDRPTYTLPQQVQTLLQPTVHLQQQQQQPFCQALHNNISEVVSMRPPDSQFTGRLDGDGALTAARICTAATEIGYPADASSVGVGAPIVNTATASYVAPLPSQAAAYGHSSEHKPPQEGIRRAAAENAGGMVHGGSGLSEGLRSLCTDLITSDVPSLAPGRSARASCTTTRGLLPGGDGRPVGAGLEAAPFKPLPSNQQNTVHHQRDQQKDPCATLHKAWQGEEQMHTLLESTTGTTTAPRSMKNKRRESNGSNGSGGGGASPSGLAVRRGAASTPRECEVKKHVEAISMFLQVFLKVDMDWTNVLRSVLKSFGTVRNILQLRDCHDQGYGLFNMMLHAAIDIGAEGSPRGVVPGGGSTGDVSSGSDNRSGSPGLEQRLIQAFEVVLERLSEPGTDIPTGEAHKMLLTQQDRECHDTPNHTCARFNLLAVQHWLLERCDAAMLVVPTKDGWLPLATACRCGNVDAAVAYLRRAVELGLFGGQGSHSGPLSPHTQQFATQVLLSDLTPVNKHYRSSNGRRVLEQAIQKVWPHGGAPSVFEGVHVAEDLKLDVEKLYNDLKSVRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB36</accession>
    <name>A0ABQ5SB36_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010739</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="170" mass="18618" checksum="82F38777EF910C37" modified="2025-10-08" version="1" fragment="single">MFENIAGRIQLLCGAVARLQNQHRKSARYVNFLSSSKILQLFMISCLISWSVLCCGSFTTRKMAHSNEVGQSVDRELSEHPYSSGFGDNDENNPNYQLYSNHFLGRHSDQVRHNSLELPESFMSAVGTAAAPTDVPDQTPPSRPLSPPTPLSPPLPPPPPPPPPPPPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBF3</accession>
    <name>A0ABQ5SBF3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thioredoxin reductase</fullName>
            <ecNumber evidence="7">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011080</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="8">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI66984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 7" id="KW-0274">FAD</keyword>
    <keyword evidence="2 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="70"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI66984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="402" mass="43262" checksum="EB87C00ED8FEBBB4" modified="2025-10-08" version="1" fragment="single">ILMANLKLVLEFVRFSSTFAPSVLPLARGSLHIQPPPGGIFEAATRHFFYPKQRHFASSSSKMVEHLYTKVCIIGSGPAAHTAAVYTARAELAPILFEGFMANGIAAGGQLTTTTDVENFPGFPEGILGVELTNRFRDQSARFGTKIYSETVENVDFSKRPFTVRTAEKEVKAETVIIATGAVARRMDFPGSGEENGYWNKGISACAVCDGAAPIFRNKPIAVIGGGDSAMEEANFLTKYGSKVYIIHRRDAFRASKIMQKRALDNPKIEVLWNSVVEEAYGNARGLLGGVKLRNLVTNEVRDLELAGLFFAIGHQPATTFLGSQVQLDDDGYIVTTPGSTTTNIPGVFAAGDVQDKKWRQAITAAGSGCMSALEAEHFLSAHPLPEGEGEVAALGETNGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBM6</accession>
    <name>A0ABQ5SBM6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011494</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="4">
        <location>
            <begin position="182"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="110"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="301"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="321" mass="34654" checksum="CDFDBD124F52CE29" modified="2025-10-08" version="1">MADLAVLRTAKQALDEGLVNADDYEFIKKGFLRAQQIKAGLDAGFIKEDDYVQARDSFLHSLDFAIVGNSSSVPPSQQSRPSSPKGASAAVQPLQPITARLTSNNPSLKFSLPQLRECPPGTQTAGPSAPQPMAPPSGLGSRTNSGQHILAAAGTVPIPTDLPSARGSRVSAIASNKTSMSGICVNEQCVAIFNHIKSKSAYKWVTFKVNDAGNEVVVDQLGAADATYEQFLNVLPESNCRYAVYDYAYQNADTNQTINKLVFVNWAPDNSTTKSKMMYASTKDFLKTYLDGLGAELQATDTREAGESEMRERVHQAITRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBZ5</accession>
    <name>A0ABQ5SBZ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Demeter RRM-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011084</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI66988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044811">
        <property type="entry name" value="DME/ROS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028925">
        <property type="entry name" value="RRM_DME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46213:SF13">
        <property type="entry name" value="DEMETER-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46213">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR DEMETER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15628">
        <property type="entry name" value="RRM_DME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Demeter RRM-fold" evidence="4">
        <location>
            <begin position="2324"/>
            <end position="2363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="174"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="377"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="580"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="694"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="906"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="944"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1011"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1280"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1493"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2070"/>
            <end position="2131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2449"/>
            <end position="2476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="185"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="377"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="906"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="918"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1294"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="2080"/>
            <end position="2089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="2100"/>
            <end position="2113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2114"/>
            <end position="2129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2464"/>
            <end position="2476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2484" mass="262106" checksum="18B3D51C68D77F04" modified="2025-10-08" version="1">MYSNQTFTDSIDLIRLTHVDRIEAGYVPLRFPGSGTPPCTVPRPGSTNLQYELTRVGPKQGLLPTMLLHGKTSRELVSEHLHADSDSLQFRIGRTSAPQPSQPPLQQLYSTTPRTRWADRMVSEQPDPINSLQHLGREQQQQHRPLLPQHQDPQHAYHARPLLYQVHLQQNLQQRQLQRQREQQHQQLHLQRPQEQRQQPQQQPRQQQQLTKQQQNNNHFPKVSRILQEDPGRIQIQIQLPALTANQHQEASISRMDCGDAVQGVFAADTKHPGVAPANARARRSERALALMATESAGATTAVDAVVRQRILRLGRTTPWNRELLLRLLLAHAAPLDAVPPVLGALTLPLPLLQCVQQQQQEQQQLLQQQPLTLKPQQSSLLQQQQPQSQPQQPQQQPQQQPQQQPQQQPQQQPQQQPQQQPQQQQEQRQLRPRRQAVPPVQLLQSSQQQQQQTQPHEPQIEEPQEPRPMGRTPAKKLTKRARVLREGQLSLIVYGYDEKILWARRVGWFIRAMRGVLGDRTLRSRWGGSVVDSVVGTFLTQNAADVLSSAAFMNLVAAFPGPRSRAFFAANRPAAVAATPGSATQATMRGPGAPLRSSPHVAHDPASPPAASPSAVTAAVMADSTSYSSYWIPEGEDIVDWEAVRTAPDEVVAELIRCRGMHQMLVKRIKAFLNQLHDGEPAVQAADTIVEDGTSKPGAEAAHELPRSKKRRGGAAASATSGFVLVKGGGGGDVCGSGAAASRGDGDGSIERKGPRISLEWLRDVNAEEATKYLMSVDGLGRKSAACITLLTLYKKEFPVDINVARVFARLGWIPIEAEATLEQLDSYPQEPEVHKYLHSRLMAFDLETLYELHYQSITLGKVFCQKRAPNCSSCPLAAQCDYALLGGQRLKVNSPGTDVVLQPPQPPLWESPLPPHHQKKHSQRKQLKLPVAPADVSMVSYTSRTTTQLTERSRSTRSPADCAEGADDEIRPQPWPQTVPATAAVVPSAAAAAQRQREVERLIAGLRAHSPARDGGGALSHGPPGATDGPDAVDGNVGASTLDVTAIDSTCAVAAAVAGCPLLAVSVASMSSLPSLPAPPPTLVNVQLLKEADAPVVAGGAGRAIGVTMAVTQPVTMEAAAEEDEGEVAMDTTGMLTMPMPSYPRLGSSVRPTPLPPKVAWTAHPSMTSGARGVDGSVELTATAAPLAKVEDVVPPPPPLPPLPPATPIKVQKSKYGGGDTHAPLRRQLPPCTSRALFGCTTYGSTAHGATEEPKSTLNAPRFDDEGHQVKVKVKVEPAATEPTSKSTARRVASADVTPAAVAAGPPEPTKELVDAPPSSPPLLAPPVAPATPMRKHKTMYGGDGVGGGVAESSWRQPAPLGRPARFSCAGAADDDDDNDVNTEAMAELLEECLIVTAPLAGTCKMQTRNIIAAAAASTVPGQQTEISDKALTMVVSGAAAAASAYDDMTVAADGDGRRGGHRRHGTKRRCVVGVDGSGSGEQMSPHTVAVAEAHPPPPSQSRSSMVATPLPQEPASRQRPSRHIRPPTRFAGPEFITFSLRRKVSRGEVHARANGRASDGGDGRAAAAAACASQCSCRSPIARDSRDASGTASEAAVAAAAAAAVATKRAVMAAEAVAVVNAMAVEAAGSFDGLPPTPPYYEELHPEVTTGSFPTAAAAENAVAAAAGTAVMDGAVASEAAVNAAVIANGTVTAAAATALKSRTRAAPAATPERLRKCSRTAAAIATRMAPDAADDAQITELEAPSDPVGPVPPTCPLAKTVTVPTATDPPGGEGPVQASWRQVDRILEIIQRYERTAVSDRAGTAGCTGGVGLSHVNDNLPLAAALEVLLTEAPAVLADGAAAIRQRYLELSVAVHPDKCRHPLAADAFAALTRALSVARRVAGDVAAAVRPGGGTLARAPDLLDDRIASISPGDGGAAATTSATNAPLYVVEDDGTIRIPAAAKKPMMTTAVPSSAVIRTSSHATGTVITPLKDLDSADLFRKRPYEGFHVGATDTALDALATAVPLPVSGSSPATAGPLDMPLSSVRVRAANAAPELRTRRLVLGLKVSDAVLRTIAPELPWPSSYGSEVVRSQPPPPPPPPGELTLRPQLPSSSPPPPPPPPPPPQQQQQQLQTQDPQPQTSMDAENESMPYMLVLLESLVAAPVLKNAVVAAMAAPTAKCTATATSSALPVLTALEVSALPPDPSVTPAAVPTENGTAVIEAAVRPLCSLGGGAPFEWRQRAAAAAVTTTKATATGTAAAAAAAISLERSWQVVAPEVAVLPALRRLSQLSATVTAAAAAAAGRIAGQVDAQHDASAVSEMAAEAAALYDTELRAAVLMPCRAALRGGFPLNGTYFQTNELFVVDHTARQPLRFRLGHLLGTGLLKPHPMLEQRQPAAAQADVHAAPPLATWASAAVYFGHATSSITRDMTAADVAALFEEGAVCVRGFCVSSGKPRPLSTFLGPPSSLGGRRAQSRKDGGNRHRVIDGEMLKEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCN2</accession>
    <name>A0ABQ5SCN2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10175">
        <property type="entry name" value="MPP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="142" mass="15119" checksum="F713DAF22DBBD47C" modified="2025-10-08" version="1" fragment="single">MAQQKTSGFSSKILGLRFMQRAAEKRKLEEDTGAAKTAAEVAGENAEMRKAPLQATAAAAADEAAWPQPSSSGRCIVMYEPVPLPGLTSRSGRMSFGPSEGKSQTLADRAPANTAATGSQPSISDSDMARTFRKPPEMRTWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDV8</accession>
    <name>A0ABQ5SDV8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">IPO4/5-like TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011915</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041389">
        <property type="entry name" value="Importin_rep_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18829">
        <property type="entry name" value="Importin_rep_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IPO4/5-like TPR repeats" evidence="9">
        <location>
            <begin position="115"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1127" mass="121625" checksum="C7A89177BFB9DB34" modified="2025-10-08" version="1">MDPAAAVAAIAANPANFSELVAQLQDADNERRKAAEAVFEALKEQPDLCVTCLVQTLRTCASVEARLFCGVMIRKVIYYRSDQEAKSPVLWDKCTSHVQAVTKQALLEALVQEPDRNVASKVCAAVSDLGNLIYDKAGWPELMPALLGMLSSNQQIQISNGLSVLSDVAPFLAEHLKSQLQDIVNMLVTFLNCGNKDVTVAAAAASTSFIEAYEDSQARQLLSPLVQPMLAVLGQLLSSGDEDEARDVLEMFIVLAESCARFLRPHLIPLVEAMMRVAGANNLDANTRQLAVEFLVTLCEAREQSPGMMRKVPNLARSLFELVMGFLLDIEDDPGWHSANDDSNEDAGSGELYDPGQEYLDRLALSLGGKAVSDAAAPLLGSWISDAAWQKRAAVFICLAQIAEGCSKIMSSASYLEQLSRMCVVGLGDSDPHVRWAACQALGQMCTDLGPELQAKHHATILPALMRVMEDFNSPRVQAHACAAIVNFSEGVETDVLPPYLDTLILKLLNLLQHGAKLVQEGALTALASVADSSQDLFNKYYDTVMPLLMHILTTANAKEHRLMRAKALECISLVGMAVGKDKFRTDARSVLGYMQSVQAAGMDPDDPLSSYMLQAGARLCKCLGSEFIEYLPLVLPSLLQSASADPDVQVVDEDEVNADDLPDDMEAISMGDKCLMYRSSILEEKATAVNMLSCYAEELKEGFWQYVGPVLKLVLNGVEGQSPLIKFYLNEEIRRSAAALLPSLLRCCIAAAEGGIQGATPAATAEFLRAAWTPLLEALRKEPEGDIQAVQLDSIAEIVEMVDKSLLAPGSIDAAFKVFDIVLEKADKRRAERASRRSTEDFDAEEAEAIDAENEMEEELFDQVATAVGAFLKKFGDDVLLLVESLLMTRYGAMLTDKSRGAEERRIAICLVDDLLENSAAGMAKHFNNVLPILLEATQADHADLRQCAVYGLGVMAAKAPVEAFRPHAATVAELMASIIRHPDAKNEDNDMATDNAVAALGRVLAHHAEALGPDGGAAAATLWVQSLPLKADAVEATAMHEQLVQMCEAQDPRIVPHAAKVASVFAEVLGGGTTYVAGPVGLRMAQLLHRLQGAVPAEVISSVLSTLKPKQQASYQAYMAGTVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE81</accession>
    <name>A0ABQ5SE81_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein yippee-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012587</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the yippee family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="44"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU110713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="143" mass="16084" checksum="3773B71E2A8830C1" modified="2025-10-08" version="1" fragment="single">ARIHAAVRKNCVLIYISFPICNLPSLAPENGMGRPFKQYLSGPRIYSCATCRAHAADHDDIISKAFQGRHGRAYLINNAVNVTLGPKEERVLITGLHTVADIYCTSCNTVLGWKYEHAFEESQKYKEGKYIIEKAKVLKEGNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEK8</accession>
    <name>A0ABQ5SEK8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012651</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044571">
        <property type="entry name" value="P4KG1-8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800:SF11">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE-RELATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="8">
        <location>
            <begin position="189"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="32"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="297"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="805" mass="86660" checksum="6E9EF8C32F3ACF7D" modified="2025-10-08" version="1">MSNVAVEHPRAFPTTAGFRVQGGFELPVQLAPPPTSTPMVAPATSNASSFLTPSQQLPKLSSVPPPSADKSTGVQFGLLLQRNLGPSSTLLGSSLASGAPMCKPVDAASELVKALSTGVPDSPAPVGWKGLLEFSETSGCSGVLGPKTSSEDLDSILNRDCKIEICCTPHTSHAVRRLVKSVIRGLRACQEPEKAVDGMGGTYFFANDSGKKAAILKPCDEEPLAPNNPKGYVGRQLGDPGWKPTVRVGEAALREVAAYLLDHDSWARVPTSVLVRARHPVFCYQSTQTDKLGPASVANSGGASGLSRPSSQACLEGPAEAAEAISPATPVLESLLPMKLGSLQEFVTHECDTSEMGPSRFSVRDVHRIGILDLRLFNTDRHAGNMLVRTQRSAASASAADLRRSMVVADASYELIPIDHGFCLPETLEAPYFEWLHWPQTMLPFSEEELQYIRNLDVERDKGILRQELPMLRPECLRVLEVCTTLLKTCAAEGLTLFDIASVMTRPFVDSDEEPSELERICAYARQCVEYTQELNQRGEEEVESCEELSDVVTEELLLQGDLMNEVFGEALHTLPLRGSTITSIKEEGEDMLFELDVDTGLRYVRKRRATAAAAGSSARRGKAADSSPDYSPCSSLSTTDECLSIQPAGSIESSDVLNGLSEAEVANASPGGDVMAIDGGGGAIPDARATVIERAVPMATSVHVTDGFLWRKLKSQQAVKRANASKGGVLTCPKRGCMAPQVYPPPVAAFSPERMSACFSGMTEEQWMQFMEVVQAQIREALDSGRWRQPAPDISFMALSCPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEN7</accession>
    <name>A0ABQ5SEN7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012759</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10450">
        <property type="entry name" value="GIY-YIG_AtGrxS16_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049578">
        <property type="entry name" value="CAXIP1-like_GIY-YIG_dom"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="49821" checksum="904A8C499D4DAABA" modified="2025-10-08" version="1">MSSFVQSLDRMLSIPNRSQASPALVRRQHLKRLPFTSICSVLNGSADRSTAKHSPTRRTRYLGCRSAAVLGAAPSAPMPSLSDLPICAVINTQGMVLPDMPEGTRGATYAIFDDKQKLQYIGTTTDLRNALRTALGRRPDKSYYYKAAALPTSEPTALQAVRDAWFSELGGQPNGNRLAIERSMWQQPVDAGAISERGRKGAAEEKTRQLLAALRDRGCKEDFTPNPTLLLEGQVDFLPARNLTDEELAAEREAEAARMRGRRSCSAIVDGQERVFHTSYLLKFPTNGGFMLDVSVLFDGRETQHRVIVGTKYYEPYGLAPEAAVEAGLALLLRAKVARATEGILLSSQFPVNYFALGQVEQWYGDEFRQEFERITGGVQLPDGNQDAWRFNRVHDYGPMRVESAEQLSLAMGQSGRTELAEAIDEAFGGGVNVAGEEEDHLPEGQGILDAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGW6</accession>
    <name>A0ABQ5SGW6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045395255" evidence="1">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="102" mass="10223" checksum="1A90897B414A1D28" modified="2025-10-08" version="1" fragment="single">KLRSVCFKAVAMGIVLDVSLGGHVPAAAVMAESETLQCAARLDVAKAVTNASGATETCASALLFLAVSALYQCNSSNSNSGFSGILSGLSSEEPSVTAHMTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGX4</accession>
    <name>A0ABQ5SGX4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="146" mass="15078" checksum="71496BD8847751BF" modified="2025-10-08" version="1" fragment="single">AGSGCRCPRRRRRGQPLRAGSGSAGRWRAQRRRQQVGRASALLPIELRCQRGGGLEGIQLTPPTGLLPLDEGSGFTGDGSRVPGGSAGRGMEAAQPAKRRAAGRGAHPVPAITLGEGAEPPEPRPSSSGDRVVPDPHPDPAGVDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH55</accession>
    <name>A0ABQ5SH55_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="690" mass="74234" checksum="8F1EFD9BCA360E6E" modified="2025-10-08" version="1">MSVAAAVPRGHPPLLLDSTLVWARSAAFTSPQAAACPSLLTSPTCARSPAIMMTAVPPPAVAKLPSYSCDEDAACDAIGRGFGFDSDADGPRVGFCPINHSYSFWGRPRDLVVGQSEPKHCGCLHVAPPGGGRGDSDKAAPEENSKTQEVAAEGLISRWHSHFHTSTSQAVPTVATAIIRGDNGRRRSFASFPATRTSSSPSETATAAVLPAMKLRQGSAGGGGGPHAALTYVPAHLAPQQPSSPALQPIPLPNGRSSNRESVSGGGAGAATPSRRPAHLVKPSAMTSQICAAQRSRAATRIQAVWRGFRIRRLYLRLIVWRLRPPPQQQDTYWGIRELPPIPDLPEVSLFTQYGRPSQFRTVKRRVWFDLEGPFWGTAAPGSLPHGSQSQSQQQGQQHQHQQQKQRQQQKQQNLEQLAQSEVVGSVAVLPPTTPEARRPRPRVPLRLAVPPRAEADVPMSEVERILAGGFWRVDGQGVSGAPAGARETSRSGEGEEGLSLYMRAKMRQEEREAEDALQMLSYEPHDLMHKLGSLKERTMERLGSLGRTIGETLSSGLLLGGRGHGHEQRAHGQQQSQEHGLWHWHWHTTDKEQAGRGVQARSLPRAGRWGISGAASGRNKLYCNGGNGLLGGGGGGGSGIEWSLRLPLLTVVGADGQSHTEVPPGAVAIPIAINRQRYRNRSHVTRMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIS5</accession>
    <name>A0ABQ5SIS5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Exocyst complex component Sec3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013920</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048628">
        <property type="entry name" value="Sec3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019160">
        <property type="entry name" value="Sec3_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20654">
        <property type="entry name" value="Sec3_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09763">
        <property type="entry name" value="Sec3_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec3 coiled-coil" evidence="6">
        <location>
            <begin position="270"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec3 C-terminal" evidence="7">
        <location>
            <begin position="734"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="552"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="671"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="872"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="880"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1118" mass="120326" checksum="8A4DC9DA458F85BE" modified="2025-10-08" version="1" fragment="single">MSGKGSIDAGLLREAEALFANSDEILIAAFHVLKVKQQGESTGAQLLKTLGGKTFLAKEAPQPRLLCLTVRRSKHSRSFKPAVHTCKPPNENDRTSAAPPPLVAGAAAAAAAAAAAGKSYPMKSITEIVVPAYKYEHNPVHLLELRFSDSGSYSTRGEIVNQFKLRSHEELQLVLALTVNLLRQYSSKLPRFTGVDVGELDTWWLGHQGAVLPVLGAFAREVLLPGGGSDGDGEDRGGGGGSRVLLSAKEERDLEELLDMFAMGVGDVAEFEARLAAEHEALEAANVHSIMVTVSAVEAVVAQIRKTQGLLEDLDEYLCVFDTKLRHMREDIAAIESSNNILELQSRNNTRLMNTLQGLLTDLRLPADAEAALESPPFGKDRRLVTITRAAWELISKLRRMDPEAPGCLSMYLLQMRVVQEARESLESMASRFVARALAHLRVVFNASVEEVVAALAANTDKASKLVPPSHDRLRAACLRHSELVRVVGALDPASMTRLHAAYSQALNMLLRKELRVSVAELRKAASLDMQANPIDYNLTSSNMARTGGVAGSVMGSRGPGGSVLGSSEDNEREDTGSVAASDTFGLGGIGFGTGPLSSGSRHSRSTFRSIVSRSGPYDPLNMAIPLHEAWQGLLEGYLPHLLAEAEGAAAFLLLRKDDVKESPAALEGAVGGTATAQDPAAISDKAAEGTRRRRKKGRGKSAAAAGDPTAVVVTQDDYGIGGGATARSHRGPLTRQGRTVLEAMLSGVDAEFMALVDLTAKSHQVLCLPMMSMATQCINALATHGSVALPLRLVLAACHARLRSHFDRFVDQQQAAVESYGSRLAASGSIKSHHVVNFIAQFALMAHHMEVMLSYSIDGLLSQLSGGAAAPEGDGRAAGGSAAAAAGPAAGGGDGGSEGEEEDEESSRRRELLQAAASWQVRPEVDAIYARLVGLMFSSLERIAAADAKHGDRLRAENYAYFVEAVRPLVRHVAALEPYMLQAETKQEEAIQRYVAAQLQYTVLWRLLEASERIDQLLQVISPGEVQYQAGFSLADMRSLIAASMKDVDKKVQKMYARVKKHLGNSNLSFRVWERIYEKFLERYECLEKQLTTCYPSLELSPTVETLEDVLRSVGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIU0</accession>
    <name>A0ABQ5SIU0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="208" mass="22791" checksum="1889866B6F8894B9" modified="2025-10-08" version="1">MSAEWKQPCWNVIAEPGGPITCVLTCCFPCITYGINVSKLQDAKDQVMCAGDFTSACCLYCCIAGVGCPCIVHIPTRKYIRNKYNVKEPEHGMLEDALMTWCCSCCAIIQDYNEIAASEGASEGTIFSDLTSAKDAATNDIKKTAKTAAKVVEHLDKKDESDDKKDDADNNDDNNDEQDNTHDNDNNNNGKTNDDKAEENAVRLGENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJR4</accession>
    <name>A0ABQ5SJR4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_014573</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.110">
        <property type="entry name" value="Pre-mRNA processing factor 4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004098">
        <property type="entry name" value="Prp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036285">
        <property type="entry name" value="PRP4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039979">
        <property type="entry name" value="PRPF18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007:SF19">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02840">
        <property type="entry name" value="Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00500">
        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47938">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158230">
        <property type="entry name" value="PRP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="9">
        <location>
            <begin position="81"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="39"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="153"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI69856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="469" mass="51790" checksum="1F9B5090FE93D9C9" modified="2025-10-08" version="1">MDALKALTSKLKKDVQDVSGSHKYVKRSQLEEARLQKIREEEAREREEKERKRKLQLNEDNLQSSKAPKHDDVPPESPTVLTREEVIRRLRAHGEPATLFAESDFDRLKRLRKAEAELAVNMEDDMTGVAGKGNALIELQRQAQEKARLRALGGSKPTEGAGGTKAGAGPGNGKGPAAVPDKGDGEQADGSSAAGGASAEDATLEAFKRAAAELAERRKEEAMPVDDRIAKYLKTWMKEWEDDLERRPDVVKDSSSGIQATFAFKQTARNMEPLYDRLRNRQITDELLTGLWMMVQAMRNRNYLHANDIYLKLAIGNAPWPIGVTSVGIHERSAREKISHVMNTNGQAHIMNDEATRKYFQSIKRLITQLQRLYPTDPSRSVDFDPVPDPGKGVQGDGCPKLALIEAELRGELPLALPTAPHFLDQDGSVRVPEKWSSILNRFRESSPSLAGVGLGDSSEGSPALGAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKE6</accession>
    <name>A0ABQ5SKE6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015383</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="250" mass="26591" checksum="17088B143EF30614" modified="2025-10-08" version="1">MAAIMKSAVRSSVRPTVAGRAARVVPRAAIEWYGPDRPKFLGPFSEGDVPPYLTGEFPGDYGWDTAGLSADPETFKRYRELELIHARWAMLGALGCLTPELLSKYAGVSFGEAVWFKAGAQIFADGGLNYLGNENLVHAQSIIATLAVQVVVMGLAEAYRANGGPLGEGLDPLHPGGAFDPLGLADDPDTFAELKVKEIKNGRLAMFSVFGFFVQAIVTGKGPVENLSDHLANPSAVNAFAYATKFTPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKP5</accession>
    <name>A0ABQ5SKP5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_015069</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malic enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF32">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="5">
        <location>
            <begin position="143"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="4">
        <location>
            <begin position="333"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="70354" checksum="11468F921A68E766" modified="2025-10-08" version="1">MLYLKLRRQLSHPVLALLQSQLTQSVSVRFIHLGDGDPEPQSEGGIPPYVPSLQPPAYVIRKAGFDLLKDPWCNKGSAFPKSERDRLGLRGLLPPRSLTLDLQAARFMEDYYHPRHLIPPEDVKSGGVTPEMARRWKVLQELQDRNETLFYRVLFEHFTEMAPIIYTPTVGWVCVNYHKLYRRPRGMYFSAEDRGDMAAMMYNWPQPDVQAIVVTDGSRILGLGDLGINGLGIPIGKLDLYCAAAGFSPSKVLPVVIDVGTNNESLRADRHYAGLRIPRITGPEYFEILDEFVTAAMARWPNAVLQFEDFSIEHAGPLLRRYRKDHLVFNDDIQGTAATALAGLYGALRVLNLPAAALKEQRIVCVGAGSAGMGVVRMIAAGMQKQGLSFEQAASRFWVLDYNGLITIARSSVAEHVRPFACRDPRHEGESLLSVIKRIKPTILLGLAGAGRLFTEETLTAMNEGCPQQRPIIFPMSNPTSRMECTAEEAMRCTGGRAIYASGSPQTPVELPDGRTVAVSQANNMYIFPGVALGGFLGQTRIITDAMLMAAAEELPKLVPKEDLEKGLVYPRLAAIRDISASIATAVALAAGEEGRLRGRMRDKFAQGPDAMRSFISKSMFYPDYKALVHLPNGLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLF2</accession>
    <name>A0ABQ5SLF2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015552</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="48"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="591"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="728"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="651"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="728"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="793" mass="78401" checksum="E3A9A0098071FCF8" modified="2025-10-08" version="1">MRLGMWSLPRATTSAKPCRAAQRARRTYQTIITTSATSIVNASITRRTHYQVLGVQTTASLDDIRTAFRRLAKSLHPDHNKLPGAREKFQEVKEAYDVLADISARADYDRELRVQAMAAAAAAAQTASGGRRASTTNVRRNRHTGPAKSQAATRPASGDDFDVFTATSASPRARSCSGSRCSPNGSGNDGYRSIFSEPQRATSSGGNNSNNYRSVFNGSSATAPVASSPPLPPRMAATPQEAAARSRSSQAGAATPGSGYKSIFDSGTPPRSASSGRTSGNAGSSNPCIFNGRAISPNLGTRKQTANYAAPAPATAAPYGSRTLSTPSSLANSSNGSRIGINAGNGTGGASEASNTAAASAASAAPSGQPAVPHAVADTLMSKPSFAPGAPGVGAAAISSLGGNSQQAAMGPASTSYTPYTVSPSIPGVSNPNPASGAATTNPAPNIVHSTAADVTSSPSGSTPHRSKGAAAGSGGPTSPAAAAAASIGTSIGDVASRLAAMTAVAAAGTPRSGSGGSGRGDYVSVFTAAAAATTQINASVASAPVARSSSPGPMINGGAVTDGTALLDAEAASTASFQVVVMPKVVLDKSGGRNSGVESASQPPLAASPPQPLPSPLVQQPQREPRAAATEAQLGVDDVPRHSRSQLTDATAASGAAATSSSALATEAEAVARLAAAMAAAAEAKAAAAAAEAMAAEAAAVAARAKAAAIAAEAQLSAVVVASAAGSALPPNLSPTTTETSTSGTVPTTADDDRPQGSEGTALPERPNRACINEVVPQVQGFVRQVGAFFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLH7</accession>
    <name>A0ABQ5SLH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1279 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045866">
        <property type="entry name" value="FAM210A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009688">
        <property type="entry name" value="FAM210A/B-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377:SF0">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06916">
        <property type="entry name" value="FAM210A-B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1279" evidence="1">
        <location>
            <begin position="14"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="146" mass="15706" checksum="10EED0F069CD5ADF" modified="2025-10-08" version="1">MASSSLASRFNFSNLKAGLKKYGKTGIYTYLGLSTCVTASFYIAIESHVDVRSLLGIKKDCGGSKLQCQSTSSYNPPWTRAEDEGVGKEPSFVERVLVGKGSNLALAFLCSKMLIPVKVPAAIALTPYVQRLIDRLAGRAARSARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLH9</accession>
    <name>A0ABQ5SLH9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015368</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055401">
        <property type="entry name" value="CEMIP_beta-hel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052387">
        <property type="entry name" value="Fibrocystin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019316">
        <property type="entry name" value="G8_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46769:SF2">
        <property type="entry name" value="FIBROCYSTIN-L ISOFORM 2 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46769">
        <property type="entry name" value="POLYCYSTIC KIDNEY AND HEPATIC DISEASE 1 (AUTOSOMAL RECESSIVE)-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24606">
        <property type="entry name" value="CEMIP_beta-hel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10162">
        <property type="entry name" value="G8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01225">
        <property type="entry name" value="G8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51484">
        <property type="entry name" value="G8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3183"/>
            <end position="3208"/>
        </location>
    </feature>
    <feature type="domain" description="G8" evidence="7">
        <location>
            <begin position="809"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="G8" evidence="7">
        <location>
            <begin position="1834"/>
            <end position="1986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="732"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1744"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2005"/>
            <end position="2039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2283"/>
            <end position="2304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3025"/>
            <end position="3062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3136"/>
            <end position="3175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3218"/>
            <end position="3249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3312"/>
            <end position="3350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3376"/>
            <end position="3447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3469"/>
            <end position="3522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="732"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1744"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1774"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1806"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="2016"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="3034"/>
            <end position="3045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="3158"/>
            <end position="3175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="3224"/>
            <end position="3247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="3330"/>
            <end position="3342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="3384"/>
            <end position="3396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="3419"/>
            <end position="3434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="3512"/>
            <end position="3522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3522" mass="363242" checksum="B4224A6B2D935B21" modified="2025-10-08" version="1">MGGVWSMVWIDGDQVSITSYSSSYGAAQYHLRAGLHKFVWLVYQTTDNDGGVWFGTPPGNQSGTPGASPGADLTKVTPVPPGTRLPVTVIINGAQSVASCAAAALDLRPQPGSQQTLDFEVPTVELSHGSEACSLVYSTYMTPTLVAGDGAAASSGVQLSLPPPAEAASGVNWVVHVYGSFLTPDPLIDVRVIVGIWLCELFNATALPYSVSAGNGAAETTQLDCTVPPLPSGEWPVAVTVGGLGAARPPVTNAPSQPVAQVRLQVADPWVAGQGTAPECQVSLFGGGTITVTGTGFVAGLRDDASGREMSVSWDASSSLGAGACVSTPAFTCTLFNLPTPPVAAIATSSLPISLFGSTTSTSAAVQIGRYSLRSLARATTMSQYGSYPNDMLYIGNSSARSLLTWRLRVTTTPAAVSSGAPVVDSGQSEVTGHVAMCGSLTPLIYDLSPLVITSDTGGGSLTVSWQIATTQLTGASGIGGVRVGSLSEASLEFETSEEAGGQVLYCTDPRVISSITNDTFYTERFTCQLPPYMPGAIYTVWLCLYPYGCGYFPSNWTVMSSVAGFESGSVMEGPTTGGTEVYIHSAGHSLDPSRIRARFGNSTCTVMTTNQTSFGCVTGPLPYEEIAAASASADPTAPLMRTLFVTPSLGTPEIEVPSAVFSFRQDLDIAVLAVSPTRGPLSGGDLLTVTGVGFLSASGVRVFIGDLECGSVKVLSNTSLTCVTPPLFSLGSSGRSGSSGSTGSRGNSSSRDPMAGLQVTAMPASSKRLTTMAGISGQGSNSGGGMGSQPSAVPTALFRYMDLWSAPGTWPMGRVPQQGEDVVIAQGTTVLLDVSPPALGSLTLYGTLVFDDTQPSLELQARSILVDGGGLLIGGPGEPYSSRATITLLGNPGDSGRYLYGSKALAVRNGRVQMTGSPRAPSYATLNATAGPGDTALLVNGPLNWQVGDQIVLFSSSFMADEVDEAVVQQIVPQSGDVVQLVLDTPLSYTHLGAVVQPPSSSQPALPLDMRAMVAVLSRNVRVRGDETSVDTMYGAQMFIHTPADLPRAVVQLSDVEFVHMGQAPALDSIRYSLHWFQQGDVSGNASGNVASAAASWLRSCAIHHVFARGVVLSGTHGLLVQRTVVYDVYGHAVALDTGMETGNLFEGNVAGLSRVSPALAFEDTTPAAFWVANPNNTFRGNVAAGSDHTGFAYVLPGTLPYTASASFSVEEVFDAVLPPGQTPIPPGGFNSNIAHSHLVYGMRIHPEYYPMTSRFGFGAIDQLKVAVFQGLVAFRNGFKGFAASSVGLVQLRGAALGANGGGPICPLSSSKDNAAQAEIAWVADPRPRLEISLQDFAGVYDSTVYGTLSEAAQNGTAGRWLAPLDRRVVGVATHSSTPSSSLSNIFDSAMALVNVTFVNFHNSSLVAAAGGTGTGSVTSSSEGQQQQQLLPFLSALESCARCMPGQGGATAFTKGIQFLSYTAAANSSRPVLSYWSWAQQGVYEDLDGTLLSPTTLGLPEAWGNGTTANISTTAAAAFPPAPVPWAGATFHSAIGNHFFDPAECLYFCGPMGQCAANTNYGAICAPRVGPFRRVFLDLLPGADVYDFTDRELVVVDSAANRSMVVPYNHWTEDAAMILVPTRRQYDIHWRLMNGSSLDTTAYTVKHLDALNSSHFLYIKTTHDREYDCVAVNNFYADVRPLPSPPLESTTHGSSSFLPIDVTGNGAAGHEWSLTTFLAGNVSDAVEVKVFDCPAGTCGCPAHPPPPSPPEPPSPQPSLPPAPPSPYLFNPGAPLPPHPSPAAASPPSPAPLPMPSPAPLPPSYGAPAQPSGGSSGIQSPPPPSSSARWSDGSTWALRSGGKPVAYDNVTIEEGWDVLLDESPPPLASLLIRGSVRFDPSVNVDLRVSRVLVVGQGSLSAGSETSPHPSLARILLTEPAPSLSSAPPPAASPNTVGPVRRLLATDNVNGSDPGLSLRSKVLAVLDGGRLELWGRRMARRWLHLAVPAAAGDNVLVLDIASSASSVGNGTNGSSKEAAEAAEVGKEEGESSTSAGLSTATPLGWRAGDRVLVTSTSYNQYQSEIRRILSVVPYSNITNDTGDANRTALVLDTPLEYNHASRIKVYGGATDPWVDVRAEVALLSSNIVVAAGEGYDSSSVVPNAWYGSPIVHNSTVGVNITQGAQLYVGNGGVVHLNGIAIEYCGQIGEEMKPCLMYDNPLSSANGTFSDGVSTAAHSYIRQSAIVSGVGGNVLVSGGNLTLDDNVFFASYGSSNVHLLTSGNTLTYNLALGAVAIGTAANKTVTSQTGSTGNGTAAVNGSSRSTTTADPRLANYLIQKPNNWVQGNVAAGSDRFGFVLYGQPCSDVDESAHGATPPPSPSSATPLTHGSVLDNVAHSCLAGMWLRSSAASRAANCTALSSFIAHTNWDFGIITMRGIDTDVRLYDVKVYDNKHAGASLLVRRDMFTTPRVAASWRGGLLAGRSSHDVCALCTAPSDAGCHQRLVAGVSYNRELPYSPVLGFVAATFAQGFSMGPDIAPWDAVQGYGLVYGHTNISNVIVADFAGSGGCGGGEFAFANNPAAPDAFHPHYFSGIRVLGTNISGPDQGLFFLTGPNPAWRNSADCGTSLLTINLTSTSPEVITLLSNTSSNGSVVYITATTSSQTTTTTTTTNANGGVGSLTALAGNGSIVTPLVVSLNCGGPAHVFFGDMDGSLLGEPGAVVGGYPMGPRSAPVAQGSLLTLPDSSGRQTCSYSSDYGSYICTGSSVLGPHLFVLASRDNNSETQGPDAVLFESEGAVDVVVPAMDQSWCFGSTECRRRLSAFWTYLPGNASTRVTFIGVPPSVSETWLPSAAPGTELLLSIQYPANSTARRFIWMADTGRLQQASAAPSLYDGSSHGSYYWDPAEAVLYIKLMGGGSSLQIRTESAAVVSLVLDMHIEDFTSERRQRFLTGLKNATGILQSQLQILRAVAGSVQVNLVVYGDPQTTSGPLPDPWLPWSAAASLDVTSQASLVSQFAGLTAEQQSELLAAAQAAATATATDAAANAADGFSSASPPSPLPPAPPPSSSGTAGSVQSPPPPVAAGIPSDLMSALLRLVSVLSGPNASTATGAPVQGLPQLVAVYGNSGGDLLSDPGVAAKLQADVSSVIQAAASAAAPPSNAIPSTGPPPPPVTAATPSDVTHGSPTTPAEKNNTGSGNRIRLEIVLPVVLGGVLIVGGVIVAVVILYVVRRNTRRGRGTGAFTAQQHSVSRSHPSSSSPPTSQPVVPSIDCAGGGPFAPAGTFIAMSAGLASPSAVSFRSASFMSSRRASSPAGDGGAAATFSTAVQSHVGSHGASAVFPQPMPKSLSLTQQQQQQQQQLQPQGGATSAGVAANPLASAGVPVGEAKRPITVPTARAAGGASHPLANSSCDSGSYLTGSDPAALGPLRPPPPKPFTLVDSPRAQPRLQLQQQQSAGLSDGPSTGFVDADRVRGPLMQSQSQAGSQQYWALPPASFCGSRGPASVGSTALDDAGLRPGSGPGPGSGPNPVEGPVKIENRRQNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLJ4</accession>
    <name>A0ABQ5SLJ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044193">
        <property type="entry name" value="TRL33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47571">
        <property type="entry name" value="THIOREDOXIN-LIKE 3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47571:SF1">
        <property type="entry name" value="THIOREDOXIN-LIKE 3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="48"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="145" mass="16662" checksum="9D459E775F79E8B1" modified="2025-10-08" version="1">MTGYPTYSWPSRPLQPPTVSMVADGTFKQPQPKDRPAMIANHMAGIRTDDEFYGYLHEYKAHTAVVQFGASWCTKCAEFFPTWYKLSKEFSQLKYAVAQVDTLKDAVAGVKYSPSFHIYRNGKLVDRVLGKEQQRLEDHLWLHSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLY5</accession>
    <name>A0ABQ5SLY5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015399</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045041027" description="Thioredoxin domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="10"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="139" mass="15317" checksum="8735F9C8D7413979" modified="2025-10-08" version="1">MSRSALLAVLAAAVLGAAFASEFTSPHVVHLTSSNYEEKTGDGKVYFIKYYAPWCGHCKRLAGTWKELGEAFKDNTNVAIAHVDCTDDRDVCTSAEVRGYPTLKVVYKGEEIEAYRGSRDKDSLKSFLEKVVEKLTSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM49</accession>
    <name>A0ABQ5SM49_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_015650</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF11">
        <property type="entry name" value="PEPTIDASE S54 RHOMBOID DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="8">
        <location>
            <begin position="144"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="257" mass="26780" checksum="5D422AC8873D194E" modified="2025-10-08" version="1" fragment="single">MQISISSHNLKTTIRRHGNLTWEPLASCLSRKAPYLRPADPNWSATFQPDASASLSLATRSTRSVRTAAGRDISITPWSGPPERRPHKHGSSNITPQPSGDGPCLDMSSGIASLIAFNAAIFAGAAFAKLPPLAGLALYNSGGTWLQYGTSAFVHANLAHLSRNMFMLWLWGSTLRPVVGSPGVWLAYIVCALGSNMAAAYALGAKAKAGTALLGVAASGGCAGVALAALALTARPSLRWLMGAVLAVQFTFPALLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMA0</accession>
    <name>A0ABQ5SMA0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047165164" evidence="1">
        <location>
            <begin position="27"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="111" mass="11713" checksum="A14D5D963E196E86" modified="2025-10-08" version="1" fragment="single">MARSTHLVSVSVAILLLVVWCKPAYTQPLVGMDLSGFSGSLGPLSSNIPGVAMLDNIGTGETIANVLSTMGVNNRVIESIVTPITNSRDAANTVRDMIMTNVANRRDAVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMB8</accession>
    <name>A0ABQ5SMB8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="492" mass="51752" checksum="500E1DCFBDFC19D3" modified="2025-10-08" version="1">MAFAHWRLSDLYVKNGDKAAKDKPLPAPSAPPFSSDGKALERGHMRSEGRSVSPTSSRAAQRARMHEPEEGCPGLLIALESADDVACGNEPGPSRCPGIHPFRYLNLEMCPSFSEQQNKMAFLDRHYTLQRVHSAAEKRKSGLCEVSSLHSVSERNDEAESGSFCDDILPGPSDTEFLLGWDLTKSKMWLVVGLLLKAARLSALPADADAYTNGRNPSALERVVEEEDSPDSTVEPPDVVAACETASRPRGALDRWRSVMRRARNMSPSVFSTPLGMGFTRNPSMSTLQSNRSFSGRLGRRSSIGPASSPAPLPCGGLDRHHVAYAAGLAAATSAAAAISHAATVAAAGVSISAGPTSASAALELGTKALGNCGGLLVEKVQGVAARHATAAVAEMVLGPQSCEVTRADLVKWVQATVAGASVMFFIGNLAAMSDALMHLDFEHNFTGSAKVVLDLVTSGSGFVENCKDLMCISVAIGVEVAGGSNTTGFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMD9</accession>
    <name>A0ABQ5SMD9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015533</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="985"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1059" mass="111732" checksum="2E7D5C776A60EA97" modified="2025-10-08" version="1">MTLYGKLSRKNEFNLTAVRQEPLSTPFSWAGTLSSSCQYQIDDLRHAPSTFTAFAREHQVDHADHIAAASRARCDTESQDVKKSSYQTQQPHHAGTLGSGRGMCCNSGTQPGFPTEDPSTAPENEHLVQRSAASSLRRQAGRHTEHDINAIRGSGSGDRGSCQSVSRSTPNASEARDQPSSDELQARREACLSQEDAILAVCSCSSDSNCRNVCSVDDLEARCAAVTSVAIVERISFLGTAPPETCHLQGSLLQREKQPPEGKQTISSSNAVRCATREARCCSSAAADTSSRDLSYAATAASRLPWWQVISVGQWPQLLSSPSHRLESFTWQLAGVQTSRIVLLRRLLLVVAALLTAAAMPTVAAQCPPMPQLSSWVTNNCTYSYQDSLCFFSTWCLKCVSPPDMAFTLLDLKLCSQDNKLAVDAGCDGISFCVAAKPSAPPSPPSPPRPPLSPFPPGLPSLPPPSPSRPPRPPPPPPHTPFPPGAVMPPPGPPPNRPPSPRPPKPPPPPPPQPPSPPQPPPPSPSPPPSPSPLPPSPRPPPPPPPPNPPNPPPAPSPPPVPPYSPGSIVPPSPPSPPSPPPRPPSPSPPPSPLPPPSPSPPPPPPPPATLSPSPPPIPIPPSPPPPPPSPRSPPPTPPSPPSPPSQPPAPPKLPSPPSPPPLPSSPSPPSPNPPAQSAPPPPPLLFPRIPPLPPFPPFPPQPRVPPFPPLISLSPPPPPPPPPRPPSPSPPSPPRPFRPPSPAPKTYTINQGYQLRIGFGVPLIPTSSQLRAIQSAVALEFSIPTATVNVTQFMQYITSVYVLVPILPTQCDTALEVYVKSTLCTELALPNCSYVDTECVDDYGLRLPVPFYPTDDTNPGGPAVPAGGVEMAVRILINEETNNVQLVLDLFQKTITLSGWTVINPAANNLTESSSLRVIVRNPTSSSTPTLIVSETRLTSSMAGAIGLTVTKVIIDLPGVVEPIPSAPKDCPKPHLGVLCGADAVGAIIGIVLGGLVLIGLLIMGLLYAQRSKMTKVVVMDDFAWARKYAHIVPANIIASPYITQYGVTSNSPANIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMQ0</accession>
    <name>A0ABQ5SMQ0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="697" mass="73038" checksum="44AA81D3B250A76E" modified="2025-10-08" version="1">MGTVLIQHGEHGPDGGPPPRAYFQLIEGKLHMPTIRDYFRLANVALDGVVYPTDPDGFTFSTFAPSTQARVTGDPLPGGPRLTRVLLIRTNDMYGDHDQQGGKRRLTQVEENLGLWTLPPPEAQPRTLSELAAALRSFLQIESNVSWVFKYRYQNELVPIVSDSSLVVAFDYFRAQAAQVADDEQDIHQLCRLYLSANEEMAPRDIIRARITGILDAAAAGREATTPPAPAGPHSGPLPGTLQLPGADSTTVAMLGSHQQAAAAALAAAGFQYGLSVPASSSFELEAGAQGGLMGDGDGDDGEDGDTQGGGGTMSSLLGLSRPSGSEGVLGGSMGSSSGQAKKESKTRHADGRFKNEAQHQKKMADMSRKIAELFEGQIVQMVEVVDHKKINCLLCNHACTAYSAYHTDCVRKHFLRHHESALRERGVDVARWRKEYEVSQKVKRLQQQQHEVATIPIVHGIVQPLQTLAVGNLLAAGPQGLQQLALPGLFLNGLGGLQANVTVGTDAEAVQRNLQAQLLSLAAAGLPVEVQLPVIAQQQAGNGNGMAGVSQMSTVSALLLQAQAVSHDEQVQIQAGTMSGLMNAAAVGSGGSGGGAPGGLVSQLSGALGNTNNSPPGGVIALAPMARMASGTVTTEMPPPPPPLLLPPPLPPAQVQPQAQVQVPMPGGPDSTAAGHRLGALAHVRSSTMLTTMSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMW1</accession>
    <name>A0ABQ5SMW1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015774</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPIRAL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039613">
        <property type="entry name" value="SPR1/2/3/4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33403:SF31">
        <property type="entry name" value="PROTEIN SPIRAL1-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33403">
        <property type="entry name" value="SPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="3">
        <location>
            <begin position="10"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="274" mass="28794" checksum="8F85C14515E0249A" modified="2025-10-08" version="1">MTPAEVDRMLRPLTLKDLRLLCRGRGLNPAGGKEQLEDRLKQHMLTTGNFSTQVPSDGPEQAAPPGYAVAPQGYQQYTTGVHSNNYSRPGGQQNVGNFITDRPSSHVIAPPGGHSTVQLGGFAEQQPAAPYHQAPPLQAPYAVQNPNVYGATSNDMRGGSLANNYSRPGGQQNVGNFITDRPSSRVLAPPGGNSQISFGDYNAPPAPSYNVPPGHGAPYGSPAPPPYGTNSAEGRLANNYSRPGGQQNVGNFITDRPSSKVLAPPGGKSQISFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN56</accession>
    <name>A0ABQ5SN56_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WDR5-like beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015940</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059122">
        <property type="entry name" value="Beta-prop_WDR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25175">
        <property type="entry name" value="Beta-prop_WDR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR5-like beta-propeller" evidence="4">
        <location>
            <begin position="16"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="58"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="100"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="142"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="185"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="359" mass="38400" checksum="560BCF9D8613A313" modified="2025-10-08" version="1">MSKLSAVVEFRCQQVLEGHSKAVASVKFAPSGLLLATASADKTVCLWDVTTGSRMKTLSGHTCGISDVAWNPDQRYLATAADDNTLKLWDVETGACLRTLTGHTNYVFCCNFDPPGHLLASGSFDETMRLWDVRTGKCLREVPAHSDPLTAVHFAYDGTMIVSSSLDGLIRLWDTQTGHCLKTLFDRDSPPVSFAMFSPNAKYVLCNTLDSRVRIWDYERGRTIKTYEGGHVNKQFCMTSGFCTTDLGHWIVTGSEDSSLCVYDLNKQTVLSTVPGARAGGDTDAANMATADRPGGAGASQHGTFHGPSCGTGTGGCTDGHTAPVLAVNVHPSLPLIASGAHSPDNTVRMWMATEAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNF5</accession>
    <name>A0ABQ5SNF5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="363"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="248"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="386"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="43261" checksum="2ECC96357A62610A" modified="2025-10-08" version="1" fragment="single">MATELVSFRQGGGSAAAAVSPVDSENRRALDRAVGLHRTRIAEASGQQQVDHRALEGVHNAALKEAAAAFGTQARGSAAEKEVFLREKTASMAHILCLNKHAELKARADKQLEAACKSFAESMSAAEGGDLTAAVSQLRGLLHAYGMDASLGQDRFMWAAEALAGRVVLDLVALVRAGHERLAEEKAAHSQLKDVFEGDSELSRHNQAVAMVADVVRRQQLQGAEQGMAALGKRLAEESAKLSEGSAVATARDALAAAEKEVEALRRRLAVVAADCGLNAEKMVERHQADLAAVKEELREAVLAAKVARVARGTAVHALETLRAELATKTAEAEAALQEAEQAKVALAEAQCTLAALQRNGASSPLAGSDEMRTQPQTPPLGDATVGVRRSGRPAAAVRRAAATTASPAAAPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNH1</accession>
    <name>A0ABQ5SNH1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
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            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="557"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="683" mass="71409" checksum="89B1929DC382CCE9" modified="2025-10-08" version="1">MRTVLLHSAAGGELAYIDIKACSSRLFLERVCFRSPLRNCLASQARSHKRRLSKSSSLKTSTLASTNRDGSRQVATTAGNNQQRTRPTSSPGGNGGNDPDDDSANSSFQIGLDPFSQQLITSAVTACAAGTVAASANLDLISALRLDADALIVALQLSAPVLLLLLTVVSPRWAPPFKGSELERLRHNYYMTAQVLQLYLDFDDEVEDSDRSTSSTSGWEDSAGGGDERARVRRRLRLEQTVGWGTISSGLALLQAFALQPWNPPTANSVFGLALAGRSAQQLANEFLVRGAGWGLLSGWLAEELQSADASDGILFFGKVFGGPETVKYVAGLALVALALPGALWEARAARNFVRASVVGPVRDAAIFCTGNGVDEPAYCDPKGFAMMAARRPANAASVADVVTRTRGWRSGPDASYSSVSSMDLADLAEEGGQEVKMPGETQPMEQLTVSRQGKAAQTAAAGAAPQLSSPSSPSMVTSPPSTGTSSAAPSTPSSLQPRAQCQLPEHAATHSLPSSAEYSRTSTSTSTSAAAPSFLDGSSSSSNAGSGNSREAAGLSSTPSYSPAPTATTEGATTGTFSTSSPSSPMSSSVLPRVGPTLSTSSTAFEWDSEDMSVLLRVETFQDRIALWSSLYYQLLAAFAINGAFLASDCNLMASFVAAWLLRTGPLLLSELRARAAANAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNT0</accession>
    <name>A0ABQ5SNT0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Origin recognition complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_016911</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the origin recognition complex (ORC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ORC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007220">
        <property type="entry name" value="ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056772">
        <property type="entry name" value="RecA-like_ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056773">
        <property type="entry name" value="WHD_ORC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04084">
        <property type="entry name" value="RecA-like_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24882">
        <property type="entry name" value="WHD_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 2 RecA-like" evidence="7">
        <location>
            <begin position="135"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 2 winged-helix" evidence="8">
        <location>
            <begin position="384"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI71637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="457" mass="48390" checksum="E9DA27B64DB5E7DF" modified="2025-10-08" version="1">MAAKVSTVPAAAPPSQRTRGGQSSLSAAAVAAAAAAARGKRNAVQNKESDDEEDGGVNGEAEEQEDEKELQGGTAAAPGRAGTKGKQSLVAPTAAESRKRLFRLLERRIGVAAAAADGGGLMAYGSLPVKNPNKKAKLLTDLKVQFDDWHSRLRSNFSVLLYGFGSKHGLLDEFARLLAGPRNRGDAAVLVFHGYNNRCTPREVVTGVAGALMQRTFRNLGSGAATVSAAASAGLAAASGGGGGGGSGAPTALRALVDDIRREPRERHVFVVIHSLDGPGLRGDVSLLGELAACPQFHVIASIDHAMGPLLWDSRDAARFRWQYFNATTFLPYSVEISKAPSLLLDVFKSGVVTAGAGTVLRSLVLGAQEVFKVLAEHLLEDDECEGVAFAHLYRMCRERYLVRDEGVLRQHLVEFVDHPLLLVRPATDNSGELLAIPMSRADMQAVLEDLQASRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP22</accession>
    <name>A0ABQ5SP22_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_016249</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Transcriptional regulator that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the type IV zinc-finger family. Class B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04370">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47172:SF24">
        <property type="entry name" value="GATA ZINC FINGER DOMAIN-CONTAINING PROTEIN 14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47172">
        <property type="entry name" value="OS01G0976800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="11">
        <location>
            <begin position="36"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="10">
        <location>
            <begin position="215"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="255"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="753"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI71149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="888" mass="91898" checksum="D2CD7A5FBD4C223D" modified="2025-10-08" version="1">MSGPSQVEFFFCGPPLTTDSGVAARTDYAGFNLNGVEYKLGDCAYLYPEDEDLPPYVGRILACFHDRSGRASDPHCVEVAWYERRAHLEPDFQGGNREYEVVALEETDVNPIGCISGKAVVFTAGDYEEARQMAKSMDVDDWLFCRGVYKQCDSSFVPFLELAGNMEAMDGKGHGKRGSAGMLGHWEGVADSDEVKRQRVVEEQPAASVRRSGKLVSGRTCVECGATQTPQWREGPAGPKTLCNACGVRYVRAQQRANKRSTASGSVRCGYGSGGRQSRTSKVAKAEAASARAARATAANSAEEASHRPQRQAALMAASKTALYARTGVFPLSSVNLQLQHVSGNGHDTAMQAPQQQPQQRQQHDANACGIDGAFGQPPVAAGADAVVRPETPNCSSYDSCGSLQEGVTVEVVVGPVDGDAECCGGSGAGIAAAAAAAGPTHPSAVAALLPSSPPSEFPGDSGIMLPSVESIMTVAPVLALATDQLQGHQVPTLHTSANAEAAAGVLRASSGFILGPDPASGDMLFATARDCRFGGHTECFPAESEQVCPQHTVECGPPTLSGSCGGVAVADSIPGGCASLKGCRAARVVVSAPVSPSVEGSGSGGFDCSDYVGDRLLDAELRGYGSQLECGLAAGLDDEYGPLVEVEVDFGLGPASAIADGSLFGDFDHSHCDDDDDNEIQPHPQLQTQFHTQQQQRHNGDSCECVFNATDLPAVAARVAAFCPDLAPGTDARELLNYMPMLAQVAVTPHEACSSTPTGEMPAAPASAATTDEDSALPEDAVAHLVLLGRQASRAATEAMAADAAFQAVSQVLEAHQEAARKAHEAAFANLGHLKNCISTLSGGDPLAVAEMGGHLIDTGAGGGLGSILGACCCSLDAGDMVVAVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPW7</accession>
    <name>A0ABQ5SPW7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="696"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="743"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="779"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="811"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="899"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="963"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="899"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="910"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="980"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1056"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1056" mass="106626" checksum="AE42E59476F3AA04" modified="2025-10-08" version="1" fragment="single">QNTYCYDIDGSGAASSGGSGGGGDGRKIEGCICGGGGDGAKGGGGHRGDGDGLGGGGDVFCGFTNGFCFAQAGGDAAAGPLSAQHSSSTRLGPFSRGQHLPCSPTEAAVQPLSLNPSSAVRLLNIISKGSNISGASMEPPGGDSKLAGGISVIGSREAVDVGFGGEPRGGIGATSIGSPAASPACPVHHCICKDHHTLRADSGSCEVLHTRLHQLFDGPPPPCSLPVASTDPTLHPQLPEHSSGPPQRHRLQCPYLSPALQSKEQRFVQYNSTEPASPCTNISTFAPASPLAAVDSTGPMIIGRPHNAGSRTTLGGQQELNSGSGGAGSGSCNSGGGGGGRTSETMESAAPMPSPSPSGRIVKLAEKISPAPRPRLAPSCSAIRSQLEGQPVPAAFNPGPVPERRVPTRAFNGTTFRTTWSAVDFEPLDDFPSNSAMSMLAGGDGVADVGQPRNSCSISRKSASLQRRRLETVSGRGDGEEAAAAVVVVAAVAAAVPASSVADGDGDMFLAQWRAVHSSFKVDAPEAEVKATEGVRTGDVSGDFGDGADVLMQWRSSLDHPPSSSLVVGGSTALDRYAALTAHSPSWKYTAMYDMHDAKLLARLMTNRISLQRGMMIASCTGAVSRVESRQGAAAAVTAAVVTPRWRDHDPTLSSLAVTGSVPFLKPTAAVAAAAASTIPVVNSVGFDTFMANGGFTTHREKSSPARRGPAGGSDSTGAPSTALSATSHSLAIRGSGIAAAQAGELTSTRRSAPFAPPLQPPATVTFNAASAHPVGAPALAASTSTPRPSHGADVSAPTDVAAAASASAAANGCGSNATDGGSPRCRTGSRSSPYSRAEPDKQRGSRFWSRSSFRNLIGWPSNETTTWGSTAAATVAGTGGRVALGDRGIMTAVNVTGGGGSCTSAGEGQGHEGGSERRQRPTAGPQQERFLVSWKDTLQALRYQAIHGDLTPRSCNFSPRAHCGHKRATSSVITLDSSNGGGGGSSDGGGGGGGGRGGNRQGTKHMATVGAVWRRNVPSVATAARVCVAATSVTGRLWSALRSHPNRKSPPSLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ23</accession>
    <name>A0ABQ5SQ23_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047325499" description="Reverse transcriptase Ty1/copia-type domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="15060" checksum="1079DA9D0278FFE6" modified="2025-10-08" version="1">MPGKVLHLGILCVIANGLLSGGAMRNLGQLQAGSMWRQPQHRPVSGRRLWMTRLAIRVSHYLANGTWTLERPPLGTKLIPCRWVYKEVKRNQDGSVERFKARLEAKGFVQQRGWIMVSFLHQHPVAHLFEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQK7</accession>
    <name>A0ABQ5SQK7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_017203</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000125">
        <property type="protein sequence ID" value="GLI71810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="268" mass="28988" checksum="E574D1937E9F8F15" modified="2025-10-08" version="1" fragment="single">PARSGHTITSLKMMLLAKNAVAVRPSGVARRSVVAKASSRPLWLPGSTPPAHLKGELPGDFGFDPLGLGANPESLKWFRESELVHSRWAMAAVAGILVQEILRPDVFWYSAGSQVESPLGPLGLLAFEFFCMHWVELRRWQDLRKPGSVDQDPIFSQYKLPPHEPGYPGGVFAPFIPGDLAELKVKEIKNGRLAMLAFIGFVMAAQVTGKGPIAALQEHLADPWGTTIFSKAAVVPGQAVAPPCKIPASVPYNGIEIPTPCFLQGLWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLM1</accession>
    <name>A0ABQ5RLM1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="432" mass="45906" checksum="FDCD388525E318AC" modified="2025-10-08" version="1" fragment="single">HAENCDIVIATFRSSFLWFRRCLLDYMLTLMCARQNDLVIAAMGLLCELFAEKTSELLHQLMQSTHIIGAFVKMVQGWGKQARNTTPSGAANCELARGMLGLLNTMATAGGTKVCRSLDKGGVTALCLQLVEECPQQQSLVRGAMALLSVMVLQVPAQRLEFEKNQGVQRLLQLLNQGMLDLSVRGMAAEVLSSAASSSPKVADTIRLQGLATVTDLLASYLHEWLVQGVEDPCSESAEMESSVPTPQLPPCNTEILEPLLRLLATVTTSSTGEVPLSDQGLKLLGVAVRAGLAHTAGEGYKVATAAAQVALHLSCKTDVPLSPALCGDDVTEPMIEMVDLACRRAAAGDVKDARILHGGLCAAGFLSKVLGARLRSNDVQLAEVLNSDLLIKLVTLLGRSADAVLVAVNAMEPCYTEPITPEGLGSGLRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLS9</accession>
    <name>A0ABQ5RLS9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000154</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="160"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="228"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="40364" checksum="EE4EFE8061A574E4" modified="2025-10-08" version="1">MVFAMKAFEQHSCKMKPQVTYQQLTSKRFLCASVPVSQACNSSVRRQSRPDRQPEPQPQPPTLTGPRRRNMLLALIPLLATDAAFAFDAGPEPLPVPVIDPSKAPDQSSYDPMDPDLREAAALLQRALNAPSVDEEEALWSEVIKRYGDMDKTWVPDVVGRAWGNRGNARSRQGRLREALSDYNAAIAICPWSVDPVLNRGVALEALGRFEEAVVDYRAVLAAAPLDPAGWNNLGNATGGLGRWSEAVEYYGRAAQLAPNFAFADANRAVALFEIGRTDEAIREMRTLLRRYPDFNDMRAALAGALWAIGKEGDAENQWARVDDPRYRDSSWLRRNRRWPPRMTESLEAFLKLTSLPAARES</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLY1</accession>
    <name>A0ABQ5RLY1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="41772" checksum="ACE9D814AE06CBE0" modified="2025-10-08" version="1">MAEEGEVSALVCDNGSGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFFNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILMERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSALEKNYELPDGQPITIGNERFRCPEVLYNPSMIGLEAVGIHDTTFNSIMKCDVDIRKDLYNNIVLSGGTTMFPGIADRMTKEITALAPSAMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKSEYDESGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMJ8</accession>
    <name>A0ABQ5RMJ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll b reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000568</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052625">
        <property type="entry name" value="Chl_b_Red"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24314:SF21">
        <property type="entry name" value="CHLOROPHYLL(IDE) B REDUCTASE NYC1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24314">
        <property type="entry name" value="NON-SPECIFIC LIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="52451" checksum="F4984A0B06EE11D0" modified="2025-10-08" version="1">MQRRTAANPHTGSHTCAATSPDISVRVQARRLLSSRRATALCRASDGDSAASTSGRSDLMKQLLRGPRPDLFQAHPKMRQLHHILRGNHSHLRGLERPLGIVVLPAYVWGTVSSLLGLQLVLPVLSGAAVGGLFAAMNRFCNRRVWPAPRQPITVIITGGSRGLGKAMAREFLAAGDRVLITSRTQEAVDVAVSELREEVAAGTLGTRGSVDDDEDAGGGKSYPQVVGVVCDVSEPAAVAAVEAAALKAFGRVDAWVNNAAYSGSFRFMVEQCDEQIEQVVYTNLLGTLLCTRQAISLFDRQREGGHVFNIDGAGADGFPTPNYAAYGATKAGITQLTATLQRELAGTPVRLHTVSPGMILTDLLLEGASTANKQAFNILCEHPETVAAFLVPRIKSAVARDSSGTYTRYLTPTSVLMRLATAPARLGRFFDGAGRAVYPSERDRILGRHAKATARRQAAARRQNGGLALAYNLSVLAGVLVMLAEAPMLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNC4</accession>
    <name>A0ABQ5RNC4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000903</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF63">
        <property type="entry name" value="20 KDA CHAPERONIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="238" mass="25990" checksum="B908B740A847C3DA" modified="2025-10-08" version="1">MQTCISRRGVFGGHAVSRRSSVVVRAEAIAVPAPFTKVDPKGDRVLVRVAEEEVKTRGGIFLPPSAIKKPTSGEVVSLGDGRLGNGEVRPFYLKQGQTVVYSKFGFMYSDLKLSNGEEYILIREDDVIGIMPRANPQAEDVPELQPLADRVLVRVEEVSDVTLGGVILPDSAKERPLSGTVVRTGPGKYDKDAEGKRKAMTIHPGDKVLYFKYAGDNMETPEGAKYVVLREDDILCKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNE1</accession>
    <name>A0ABQ5RNE1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_000598</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000:SF642">
        <property type="entry name" value="INACTIVE LEUCINE-RICH REPEAT RECEPTOR KINASE XIAO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI58834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="110" mass="11412" checksum="5149B79B1DC28BE2" modified="2025-10-08" version="1" fragment="single">LTGPLPGMWAGLRSLRELRLRGNNLSGGCLPASWGGGLGSLQDVDISHNPQLCGDNLGASSSSLPASWGRLGFLRSLALQNTGLVGTLPSEYSSLPRLVQLDLSVNNISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNK2</accession>
    <name>A0ABQ5RNK2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centrosomal protein of 19 kDa</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_000652</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029412">
        <property type="entry name" value="CEP19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31539:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 19 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31539">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 19K CEP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14933">
        <property type="entry name" value="CEP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="130"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="208"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="373"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="215"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="448"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI58883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="460" mass="48424" checksum="943E9534A73EA552" modified="2025-10-08" version="1">MPNTASSQTLNPAVENGGSRKETTAESEGSYKAKRYAVKFQPPCIFLEYEDTTRRRRVRAVKLNGVQPNVDVDRLTKKVIRSFPRRLDPTSVKYDQVRRLVVSLVDFLSAGTHGANGVVAGAGAAANGSATMRNGTSSHVPSRLGPGGGTKVQGRLSDFNDHANPANGGASVASPLPSLSPRGVDPRIGPAARMAMAGISSNYRGSVESTMSRFSTSTASPSDSGGSTSEEETDEGKRRNGGGLASALDDLDKEMAGLGGSTSELSTRATAGGPGGVSSKLEGDFCSSPSSTRDRPLPPLGTSISPAPSRMGSMDAQAGAAMVADSCAKTRFAAEAEKLVIGLEVTNDLDLNKVTEVELKLAKQAMEEDFKKNQLRPGDPGYVYDKQEEFGPPTEVNDWDETDDDDGLSEEPEVDWADIHASVSKPKGTFTSVQVNAGLSSEEESAGRNGQSPYNSNDWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP18</accession>
    <name>A0ABQ5RP18_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA-editing substrate-binding complex 6 protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058917">
        <property type="entry name" value="RESC6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26188">
        <property type="entry name" value="RESC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA-editing substrate-binding complex 6 protein" evidence="2">
        <location>
            <begin position="317"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="835" mass="89633" checksum="94548BE8A493CA66" modified="2025-10-08" version="1">MLSYRKIRSHVSGCRWGLEQAGNVPRSAVVGRLLVANATRLRAKAEAEDEETVFSVPSGLQLKTRRSRATIPQPAPETASAIEYTGTRLHQEPSVADAAALRDATATASTSTSTSGYEPPFRPQSSLKQRKLSLRSSAASTAASAATAAAAATISTSLSGILQPEEDSPGPYLHSGDSPTDIQSISLTDHDVHEAAAALGQEPLQQRRRLDEQSQPQHEQQLQPQHEQQPQPQAAHPLPAGVTPERDREIHNELRGCEEWRDVLDIVADEAACLSVRTAVQALTRLNVLTRGSVSERREIADHPAFQTLKEVLYSQVSLMNNVQLSNSLHALAQLQVKLPELLLDGYAEAAAARVDTFYPRDIATLLAAAAAMRWSPPREMLDTMGFRSMGLLTGQEFDARSIPTLLHSIALLGYKNPLLAEAAAQRLAAPGVMASLAPQGVANAMWALGRMDTYHPPAVEAALSAFSANPMSYKPQEVANLLWALTAFRHHPEGAFVLFARSLLQRQDGNQIRAADLATLLYAFALFNVGPGARVMERVEACAMRLLEEAAAAPQPPPQPRASPPRGGGRRGSISVVELAHMYWGLALLGAVEGPLFGRLEAELGAAWEREGPEIPQTLLRVTFQGFLASKLESHRRSHGFNELALDALKRAWVAGLGADSQKYGRGRRVDQLAAILDTLKVTYDKRRPTGDGLAIIDIALKAGPERYLALQVVDTADRCANSKQLLGQVVVTGQVLEKNGWEVRHLRASDLDSLTPRMQTRFVEDLLRGMGVRVRKASAAAAAAAAATSSSGTSAAADGLARYGGARGAVETAVKEGGAGAAASRALKTRSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPM5</accession>
    <name>A0ABQ5RPM5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GPI transamidase subunit PIG-U</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_001352</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009600">
        <property type="entry name" value="PIG-U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS CLASS U PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06728">
        <property type="entry name" value="PIG-U"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047480865" description="GPI transamidase subunit PIG-U" evidence="11">
        <location>
            <begin position="26"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="480"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="521"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="553"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="594"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI59469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="65042" checksum="7AAA75B928F3A46B" modified="2025-10-08" version="1">MLHAAFVGTSARVLLAWSSIGASLAWRIEVSTASNSNLELREGLALLRLGVSPYSGTSCRTPPLALWLYGALADHDLLYILPNTILDLLAASLLYRLASYLLNYSRSRGKSVETSEDTPVYDSVNGDCFAVKGSFSGFRSALPSALAWCYLLNPFTLAASAGGTTSALENLALVTALYGACTAQPALAALGLAAASYMSLHAAVLLLPVACLLAYGPEDVVTPLRLLGPCHNGSALHVGAPGVGVGAALGSGDGGTAGGEDSPGKAKAALVKEGKLLEEQQQKLARNEMDRAAVGEVRDSPSGRATLETRSLPQCSAPLPTPPSLPILPWRQLSQCTLLAASILSLLVCASDLYLLSVPRRMGIGVHADADNGNTTTCILGAAKQLITPPSTSTGTAAGEVARRLVAAGESLASCWAVRVYSSQVLLDDATPNIGQWWYLAMEAFDDTKTYIRVMAHSLLFALAPPLAMRLGVRRPLALFLIQMLCLGFLKPYPSVADLGLMAPLLLLLGRQQLAALRLRLLLPASLAMLWVLGPAMQRMWLSYESANSNFFYSITLLYGLWQVVLLGQVLGLTLWVDRLARGKERLLEGRLDERRQEAQMSSAAPEADGAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPU5</accession>
    <name>A0ABQ5RPU5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="235" mass="24862" checksum="19F83FCDA6EAF6FC" modified="2025-10-08" version="1">MASTSDERGGPLDTFAEDLGRAVLDAAVSGNLSAPALLAVTTETGLHPDQLATLIAQTTEIRTQLGKYLLKLLNLLKSTVAVNTNMDNAADPRGLPLPQTQGHAAIPEDPLGEAVALDQEPGATRPAPTVTARCSNRQRKVRGGASSGVHRSGRAGPSSDPAKSRKRGGAPSTAAAQRSVPDAKRTHTDDPLMPKWNCNQSEFERRKAANVCLRCMESHPTKDCPLLPPFVPKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQD5</accession>
    <name>A0ABQ5RQD5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Saposin B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36058">
        <property type="entry name" value="NUCLEOPHOSMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36058:SF1">
        <property type="entry name" value="NUCLEOPHOSMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045787917" description="Saposin B-type domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="33977" checksum="DF14BCE3604F43B3" modified="2025-10-08" version="1" fragment="single">ICVSKMIRLFHVLLLTFIALASAQFGGPKRPKPKAPVNKDDIKYIKCQVCEAMAKEARNIAKDLGQLAGAKKVSEADILERLEKMCNPDQNEGDWITTYDIVEQGADLHLKDTGMVGKCKSKCRTIARTCELIAEDLDLTDLSAMLYKGKKRSAVTNWMCYDATDVCSSKPPPVPAGRPAGEPHEPLDEDELRNMKMMRDMEASGLSGSLYSRDTLTQELEEMQDMYGDDPDFAQVMKDTGMESFVARKPEEEPVGGETAVDSSKVTKLQETAAKAADNIKEGAAKLVKGAKNFVGKLFGGSKKGGKSGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQV8</accession>
    <name>A0ABQ5RQV8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001983</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037494">
        <property type="entry name" value="RAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040858">
        <property type="entry name" value="Raf1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040781">
        <property type="entry name" value="Raf1_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041358">
        <property type="entry name" value="Raf1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35299">
        <property type="entry name" value="RUBISCO ACCUMULATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35299:SF6">
        <property type="entry name" value="RUBISCO ACCUMULATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18579">
        <property type="entry name" value="Raf1_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18578">
        <property type="entry name" value="Raf1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18087">
        <property type="entry name" value="RuBisCo_chap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubisco accumulation factor 1 helix turn helix" evidence="4">
        <location>
            <begin position="133"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Rubisco accumulation factor 1 alpha-helical" evidence="3">
        <location>
            <begin position="205"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Rubisco accumulation factor 1 C-terminal" evidence="2">
        <location>
            <begin position="330"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="516" mass="56059" checksum="CCD21C315A524F31" modified="2025-10-08" version="1">MVLPSCPSLAARSRCHRVAVHARFGRDNGLILGPGGPNNNGNSNQRSKEARLIIPGQQRQVPGGRLQRLLTPDKQQGGLVGGDSSFLPDDSTLGLVSEQGGLVGGPPTLNKYRPPAGFMNETLPDDASVNIEPQEMLNKLRARAGHWHELAKFIPALNSKGYNSSVIDEMTGITPAEQNKWVVSATVYESVKAAPSVRADVLRRFDGPGVDLLYPFRFLSAERRVAAAQYIVEQDLDPAMCEVLARSMKEYERRVPERAGFTDHPADCLAFKYLRDALECRKREDAMVKLELGLQVALSDGARQRLLELMDENPDADAMDSPGVAASLVLLRLSPDELGVRPVCVPGELGQVQPEDLQAAPRTTQSGAFGIFSVSMETTSETASPSGRALSTSWNWVALPQWRALSMARHPVALTVRDCAAVQAVLLGSKAKTDDDKRRLQGPGILVVDKAPETFEQLDETAWYLGLPEGCRTLQMVDGKRAAVLAARSSLFASVLFLARPPVKEVADRGTDLLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQW1</accession>
    <name>A0ABQ5RQW1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001821</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="cyclophilin_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
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    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="488"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="641" mass="70818" checksum="5A14DB4CA924C0AA" modified="2025-10-08" version="1">MADQDEDTVGPPRPLEGADEEQVDDQEQDVGPAPPKPKKRKILEFEQQYLDVLPSAAMYERSYMHRETVNNVAVTSTDFIITTSIDGYLKFWKKQPRGVEFVKQYRAHVGAVDGLAVSGDGTLCMTLSRDRTVKIFDVLNFDLIVMLRLSFTPGCAVWIYKKGEAKARLAISDLNSGRIHIFDARSGSDASEAVVEVHRSPVTAMRYNAPLDTVISTDAKGMIEYWCGSSYGHPDGGRVAWSSKLDTDLFDLTKAKATALSLEVSRDGQQFSMVCSDRRVRVFKFGTAKLRRVYDESPDAANEVQRSGGEMFQLEDIDFGRRMAVERELAASAEETHGSPNAIFDESGNFLLYATLLGIKVVNLVTNKVARILGKVENTERFLTLALYQGAARGKGAAGSRLRGLPTAEQVDKLLGASDPMVVACAFRKQRFYLFTQHEPTEPEDPTQPGRDVFNERPALDDLIVAEGGAPAPGHSLPHGARLHTTRGDITVKLFPDECPRTVENFTTHARNGYYDGVLFHRVIKGFMIQTGDPLGDGTGGESIWGGEFEDEFHKALRHDRPGILSMANAGPNTNGSQFFVTTVPTPWLDNKHTVFGRVVRGMDVVTAIERVRCNKDDKPFEDIKILNITVMDTVDETGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR28</accession>
    <name>A0ABQ5RR28_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Pesticidal crystal protein Cry22Aa Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_002033</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032179">
        <property type="entry name" value="Cry22Aa_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013122">
        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16403">
        <property type="entry name" value="Bact_surface_Ig-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08016">
        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="2215"/>
            <end position="2236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="2270"/>
            <end position="2301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="2409"/>
            <end position="2429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="2450"/>
            <end position="2469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="2528"/>
            <end position="2551"/>
        </location>
    </feature>
    <feature type="domain" description="Pesticidal crystal protein Cry22Aa Ig-like" evidence="8">
        <location>
            <begin position="606"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin cation channel PKD1/PKD2" evidence="7">
        <location>
            <begin position="2403"/>
            <end position="2549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="225"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="290"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1435"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2886"/>
            <end position="2930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2948"/>
            <end position="3022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3309"/>
            <end position="3329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3375"/>
            <end position="3449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3528"/>
            <end position="3855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3888"/>
            <end position="3920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="3938"/>
            <end position="3997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="4039"/>
            <end position="4215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="290"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2907"/>
            <end position="2921"/>
        </location>
    </feature>
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        <location>
            <begin position="2977"/>
            <end position="2989"/>
        </location>
    </feature>
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        <location>
            <begin position="3006"/>
            <end position="3015"/>
        </location>
    </feature>
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        <location>
            <begin position="3410"/>
            <end position="3419"/>
        </location>
    </feature>
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        <location>
            <begin position="3537"/>
            <end position="3546"/>
        </location>
    </feature>
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            <begin position="3553"/>
            <end position="3565"/>
        </location>
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        <location>
            <begin position="3633"/>
            <end position="3652"/>
        </location>
    </feature>
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        <location>
            <begin position="3660"/>
            <end position="3672"/>
        </location>
    </feature>
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        <location>
            <begin position="3686"/>
            <end position="3699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="3728"/>
            <end position="3738"/>
        </location>
    </feature>
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        <location>
            <begin position="3748"/>
            <end position="3758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="3892"/>
            <end position="3906"/>
        </location>
    </feature>
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        <location>
            <begin position="3938"/>
            <end position="3966"/>
        </location>
    </feature>
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        <location>
            <begin position="4054"/>
            <end position="4066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="4098"/>
            <end position="4112"/>
        </location>
    </feature>
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        <location>
            <begin position="4113"/>
            <end position="4122"/>
        </location>
    </feature>
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        <location>
            <begin position="4129"/>
            <end position="4142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="4166"/>
            <end position="4177"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4215" mass="448951" checksum="5B566D4FF07A20B7" modified="2025-10-08" version="1" fragment="single">[encoded data, 4215 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR35</accession>
    <name>A0ABQ5RR35_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002090</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI60025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="60"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="486"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="502"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="607" mass="64758" checksum="642150B7FA408274" modified="2025-10-08" version="1" fragment="single">MEDVKHRAEALALSSPLLLPYERQIVEEATQDDCLIITAAGLGWQRVAAVLLRIAQHARTAGTPSPPALGPQDQQNLQRQEPPQNFDQSAASASTDPSAAASTGKAPGPIRGLPGCVLVLGASPWQRTLITEELARHDRSLPAPVDITNEVPAAERVQLYRAVPGGRPLFITARILVVDLLARRLGGREVAGVLLLNAHRVSDTSGEGFAVALLRGDSPAGAPGSCWVRAVSDAPVAFNQGFNKVEKVMKALHVRRLHLWPRFQEHVRQCLDEHKPVVVEWDQELSGSMVLIQAALLEVLEGLIRELRRTNNKLDTSELVLENGVLRSFDEIVRRQLDPIWHTVSWKTRRICADLRTLRELSALLLSTDCVTFLAHLEGLRQSEGVRCVWLFHDATHAIYEQARRRVYMYRQPTAGVDPGKAEAAGTTGSSSGGGGAAEVAAVLEEPPKWGLLVDVLGEVQRQRREMRALRDLHPAVLAAAVAAGSGSGARPDVDADADTDFAQSGGSSQDNGRDAAAATPGQRARATLGTMGTTTGSVEVVNLADEDEDDTEGWKAADIAGGTAAATSHAVKRPSFGPGADAASADVAAAAQSLRTLLEDLGLIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRC4</accession>
    <name>A0ABQ5RRC4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="209" mass="23217" checksum="E225C9E811F6EB60" modified="2025-10-08" version="1">MGDAAVNVVATVTMGVSSILMPKVVFKGDNWNEFRRDVENCFLYNVWASALTSLDDPATFKALIVLRRQVAQEYQIELSDCENAKVAWDHLKNIGERSSSAKLIVLMRTLHSLKMQSEENVYQYFARGKELRQMLADAGEKIEERVLVLALLTGLTNRFRTFVDIIMVSGEIPKLEVLLPKLVAVESFESQLGASGDGALALFSGSGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRS1</accession>
    <name>A0ABQ5RRS1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045359354" evidence="2">
        <location>
            <begin position="24"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="167" mass="17571" checksum="4F04E1E3BBF7F007" modified="2025-10-08" version="1">MASNSIVLGLTALLFCTWSVSLAGIASVQDKCTSNWAGQLAGINGFSNGLACMSYFRYYWFIICLEAVLIFGLGLALAAGAYAKTRLSWLGLFAVATLLYIQTSDAFLSLDSITADWGGQTKHRVRVMIAGSIMTATVNCMLIVALGIIPEAASTPGTNAEKSAVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS44</accession>
    <name>A0ABQ5RS44_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_002557</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF375">
        <property type="entry name" value="PEPTIDASE A1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 11" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="522"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="13">
        <location>
            <begin position="100"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI60406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="611" mass="66862" checksum="AD6070F1C162129E" modified="2025-10-08" version="1">MYIDIILYEYATIYFLVTDLLKSVADVEGEVPKMIVATVLFLSLVFNAASGEGVVSSSSKRPRGGLMLPLYQRSHPSWRRHLLRSGALPVQGSVREVGYFYTTLLLGTPARDFSVIIDTGSTITYIPCKGCAHCGKHQDEWFDPDASSTAKKLACNDPLCNCGTPSCMCTNDRCYYSRTYAERSSSEGWMVEDVFGFPDAQPPVRMVFGCENGETGEIYRQMADGIMGMGNNHNAFQSQLVARGVIDDVFSLCFGYPKDGILLLGDVKMPARAKTVYTPLISNLHLHYYNIKMEGISVDGETLPLDAGMFDHGYGVVLDSGTTFTYLPTEAFRAMSSVIKTFAESHGLQPTPGADPQYNDICWKGAPDDFQNLERHFPPAEFVFGGNARLFLPPLRYLFLSRPGEYCLGVFDNGMSGTLIGGVSVRDVVITYDRRNSRVGFTTMPCNEVAADLAAQPSSSPMLGNVTILAPPKPSKPEQQSPPAEVRGNFTKAQQDATRAADVKQVWQNQAASMATFNINHAILISTIAALGALFMLAEVMLLGRNTLLMQASAVLERIRGVIGRTSSREQANEAETTLSAREKITRNMDVEKASLPFTDMGKNAKPSSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS55</accession>
    <name>A0ABQ5RS55_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rab3-GAP regulatory subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="824"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1186"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1365"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="829"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="840"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1207"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1369"/>
            <end position="1382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1452" mass="150123" checksum="12D4860C64B71B7B" modified="2025-10-08" version="1">MHMALTTYQTYHAGEVYLALRRDFNWELLTPHARDTMLKCMHEEQPPKDKVVPLSRNCVDTTNDILRFYSWLYRVACDASPAKVAANNGLTCDRAFSGGRLLSYRYRMLIIVGGNQASTNVPKPPLLPPPLPLQQQQQQLKSRGKRGWEEMETSYHGGGGGDDDCFCNVLPRRGPGGGGGPSGIGRGPGGAGCSGDPATRRDELPNESRLHEATELRPLVSPVRLLLGAGAALWDVFPGPSRRLCITSPTSSASLSPPANCTLPPLEGCAVAVYGSCILVHGGISPAGEATGELRAFRLRPDLCRTGGYEMVGRVRVQQHGEGGGGAGGGAGGLPLMVAASRPQSASWLPALTVNATAAVEAVTDTLASAGDGGASRPCPPPSARHSHHMAVDSATSRLWMFGGCRGEMMSIASGGGGSSTSDWRRHDATCQQSLPAPFVRTSAEASERRADLVPCCFWAQLPAPRDAMPASSGMSTAVAAAVTTNDDGKVGGGGAAVTDRECADDDFFLGGDVVVSKVEWHCLPLRSSRRGGGVSYGNGARGTTLGPDSPDPTRVAAIAVQDGGLFVLSSGPGPAGGATAAEAAKIRSSAAPGHSEIRWWLHRIDLQTGLCELLGAPTASPVAASGGVNVAAAAAAAPPSQPPLYHDDAVAMADDILPYIYVYGSRQLCSGPAALSGGVFVPSLHRVSLDGGSGGGGGSGALGAAAGDWEAVELSGTARLHVARKALGTASGGTVILVSGVRDVRKGVEAQVQAVVPVSLKGGSAVVSCTANKALLASQGPQNSDRHVFIATALARCRRLIAERLRRAAPGVVRFTNPNAAVRDDGCRTGGGGGGGGVSRNSSSPSSSSRPCVALAAALSLYSRGGLFDEAFFEELDGDDGGGGCGMTMPGHDPLAVAAVVAWVMGRTHVRLDWEPHFLLHVFRVAHCWELAALQREIVALVSRLAPLLPLQHIPAALGLWVEMRRLGAVGMMASSSLFSSSSSLAAPAAGSGVRVEAELRSRLIKLASNCSLRPDNLEEVMEAVMAAEAENEAEEDTAAATAATAAAGREVTSRSNSSPFASLKQACLKMAEELLTPGSGGSGSSSCIGAPQVKGARAATRFACRFQIRLLLFQAMRWWRRRLFTCPQDAATLDHLLGLLSELKDMEKEGDAAATMRGPRVAPAAAGSQQDVFAPRGALNRLRVPPAPTVADSENNPLEPPPAAAPASSAPAPAPALPAAAASFGRCKAGVAAVIGATVVAEAEADARQKAVEADEEIEEDEEGGEDAVPLSCQLRGCLWGELFRCLRPHTALAVLEALDRGRCGSEATTAACEIWLYARQKAYDMLAVLSPMPRLQLPTKLWTGGARAATTHTAVVCSGTTTSGASGSGGGVGSGGKGNGNHVHTMTTSWGRGRGGPTWDGNGASAPPPPPPPHVTSLLRKAQPLSRACDAWQRAQEEEVLRRGGRGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSB0</accession>
    <name>A0ABQ5RSB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Btz domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_001941</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CASC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018545">
        <property type="entry name" value="Btz_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044796">
        <property type="entry name" value="MLN51_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46837">
        <property type="entry name" value="PROTEIN MLN51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46837:SF5">
        <property type="entry name" value="PROTEIN MLN51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09405">
        <property type="entry name" value="Btz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01044">
        <property type="entry name" value="Btz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Btz" evidence="14">
        <location>
            <begin position="70"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="41"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="182"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="510"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="752"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="256"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="295"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="432"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="757"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI59902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="776" mass="80670" checksum="7602D36914772752" modified="2025-10-08" version="1">MSTSILEDEAVLDVKVPTRRRRASDDEAEEVEFHDAAEYAASVADSQAVDQEEQGADEPRSEDGYEGIIAPTAEGAEDENVGETEEHPSEHDQPEDSAVDRKKAKLREPFEVPTSGAFWLHDDRFGGEEPKAEPSRPGGGAAERKARSDTEGRWLHDKFSTLELDDVAEDDSSDNVFLQYRRGRGRGLRGGGRRGGISGGRSPLTAGSRGRGVSAGRGRVGRPASNHIDEIISDLGNSGALPSQGRPEIQEALPQRPQRAPRAAKRSAAAPPSSYSENSGAEAAELAAASASPGPGRGRGSGARGGRNGEFSGRGRNERSFSPDAGHRERGSEPDFGSGGRGRESSGRGRRREDGGRASSVLGRDDSGRGPGRGYGRGMDPYESGRTLEAIRGSEVISSNGGTYESRRGRGRGAGRVTADDYQDRGPGSGRGQSSTQRTQQAGQQQQQRRQERSVDEEFPPLPSSATARKPAAAPSPNADMPPGVGGNIVAMDAAATATQGVRRYSKMAGKVGGGGSSVPDSSAGTGGGAAAAPESVHARGAGADGGGAAPRASKLSAAAATFEPSPAAAAAATGRQLAASPPQPALMPQQPVPSVPAAFGAPLQPMTVVGVASEQYSAGMPPQHGQHMPYVQSQQQHTQAQAQPVPGVYGGPMQQQVTMPPQGIAGGEGSPMAPSLQNMPYTSTGTIMYGAPSAEVEAWYQDAYQKALAIGMDPQQAYSAVQGYVQGLKMAAAVVEQQRWQQQQQMSGYAGVLPPQQMPTPPAGPAPYEAPKPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSL4</accession>
    <name>A0ABQ5RSL4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyl hydrolase family 30 beta sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002327</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="16002" checksum="2A958EB59AAAEFA3" modified="2025-10-08" version="1" fragment="single">RYASHPGSPTGFKNVGMTPRWISTSPVAFAPIGQQFVVTLWQNATFHRAFQVSAEQLQAATAAASAQGGTSVAALTSVADDVATVFLNDRPLGQAAPLPSQSMFPTPTELLRADHNLIVVCASSAGGPSVVVVPLIAANGSVLVHTDAFWNWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT86</accession>
    <name>A0ABQ5RT86_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="542" mass="59333" checksum="00E37A511319CE3D" modified="2025-10-08" version="1">MQRTFNWVPKVDPVLEVLLQAGASPNGRNSLNMTALMLVATSLLAQLECHVAFSSCQGEDLELERQVAQAVAVAAATARLLLAAGADPAVEMRGFGTRWDAGSRSAELLPAPIRFARYVLHLERYTVHQAFEVMRHTLDGQIQTTGSSGSAQCLDEFAALLLPTEAPGAAAATNAGVYSSGGLSTAPPGDGPSAAAAAGVVGSDTHDAKKPAVVTTGGYANSDGFYVGKDCGSSLDTCKEATPGFRESDRLRDDLRPQQLPQERGQHPPERRQQYLQPEQARLQVPVAARDSTWPWIQAMSNIVWAVSQVAMVETAPGRRAFLRQLVREAPFCLPPMAVVSAAQLRAEGCIPRYSRRRELREVLPSTPSSLSPPEPAYQPLAVDDLPPSAVVVFVSHRWLGRGSPDDDHRTKLKQVMAIAAHMSKRLGVPEDDVYLWLDYSVIDQDNPMPGVQALPVYIACCEEFVYIHHDEYWQRAWCLTEQFMHWKLATTERKHALDPHTLDLTSENAWERPPDPTYGKLAVEADRVALATMTSILPYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTB1</accession>
    <name>A0ABQ5RTB1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U6 snRNA phosphodiesterase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3'-5' RNA exonuclease USB1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002722</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027521">
        <property type="entry name" value="Usb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522:SF3">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09749">
        <property type="entry name" value="HVSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="317" mass="34354" checksum="7564D3138C6E7E09" modified="2025-10-08" version="1" fragment="single">RVEMYQLLQGYASSDDDDATSSHQGQDETVANTLTANHDFYEDDIINKRNQNCGSGLRSSRCPITKRPKLESGLQLTALTRVLPQSTPAPAHMTEPTALRTALPNPLTLLEAPSTEASQDLLATAAEPSLLLTSALPGPPRVRTFPHVIGNFPTVVLLPVPGMDDALDAVYRRIRRLLPDLEPMTHDPPPESAGTGPGPYCRTGERSVRGGKQESPGPHVLQVTTVQPHRGYHISLSRTVAIRHPQIAPLTALLVERLKVIGSCFQVALAGLRSFTNDEGSRSFVSVMVTHGSSQICSMISQVDTVFQQHGLPAFHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTL6</accession>
    <name>A0ABQ5RTL6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="133" mass="14000" checksum="77EED8378D3B5F56" modified="2025-10-08" version="1">MCAVGKNTRRCIWGNLQCVLATDAGALEQSAMCAVGKNTRRCIWGNLQCVLATDAGALEQSAMCAVGKNTRRCIWGNLQCVLATDAGALEQSAMCAVGKNTRRCIWGNLQCVLATDAGMAKRGTRRGTGTGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTX2</accession>
    <name>A0ABQ5RTX2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_003006</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="479"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="706"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="713"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="751"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="774"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1361" mass="150299" checksum="4C42B9E7E9BA69F6" modified="2025-10-08" version="1">MYGIQKVFTMGKDRLDEPLDLHNYSTWKLRFQLLCAELGYSRALLQAPIAEADVIISDKVKSVMAKNVKNHHLQTIVRAANAKAAWDALAATFASTCNTRKVALRQELSDFKMANGEELPIYISRARQLQSDLLDVGHAVADTELSTIVIKGLPSKYHVVTTALTMREGELDFSQVVARLMAYVIPDEDNNESSSAFITHRSGGKFNPTSRHQTSKQRSKSNAICHYCGKPGHFIGVCKKRLADQQQQNNGRGGGNLQRREPTKMAYAVGSESRSDAWHLDSASEWHITYDASELKDLRAVRPEEKFTLVGYDGSRHQPTHVGQYTMWSNTVKGLQIELKNVYVVPKATVKLISAGIFDERGATILLGNDKALVQAKGRTILRADKVGRLYAIRHHAAGRSVGKGRSCAAVGSEGSKAALWHRRLGHLGYKSLERMCREHMVTGMDLDRASLEAARSRICEPCIYAKQTRGPFPSTGHKAAKPLGLIHLDVCGPMPETSIGGSRYVTTLLDDCTGFSTVAFTDTKDAVRKKVKIMIEALENMTGRRVKEARTDRGTEFLNTVLGAYFGEKGIIHGTTVGYSPEQNGAAERLNRTLLEKTRAMLAESGLPQKLWAEAMATANLLRNVSPVVGASVTPHEAFMGVKPDISHLRVFGSTAYAHVPKEKRNKLQPVSKKGVLVGYEQGRQYRILINNLISTHSTVKFDESTVGDRYEDSDEEDVEESGGVPSPGGGGDTPVNTDMDQGAAEVNHGASTSSGQTAAPQRHAGIPAAQFPRTQNTAQSRGGNGGDAMTGEGPVQQQNHNNDGADVNMQERRYPLRERKRLIEWRHEPNGRVTFGRINAAIEEEVPEPASYQEATDGPYAEEWRSAMDEEISAQLTNGTWELAKPPPGARILPCRWVYKVKRAEDGEIERYKARLVAKGYEQRAGIDYGELFAPTSRFASLRMLLAVAAAKAMPIHQLDVSTAFLNGELEEELWMQQPPGYESADPEQACRLLRSIYGLKQAPRCWYMKLAAELDKLGFEPSVADPALFIKKDEKEIVYLLVHVDDIIITSEDEAMIRRVKTEIGQAFKIRDLGEAKVFLGMEISRGAHGEVKLSQRRYIEELLQRHQLVNAKPRKTPLPPGTRALPAEDNNKELVDNTKYRALVGELNYLATSTRPDISQAMSVLARFMGNPSKVHMGLALGVLRYLAGTKELGLCYDGDGDLTMVGYSDSDWAGDPATRRSTTGYVFLLNSAAISWNSQLQRTAAASTVEAEYQATAAAVREALWLRKLTRDLGLRTQAIQIRTDSEGSMSLGNNPIMSVRSKHIDVQHHLVRERVLRKEVALTHCSTEDMVADVLTKALGEIKFKKCITAMGLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV25</accession>
    <name>A0ABQ5RV25_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="79"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="817" mass="85079" checksum="120D76E7650967FB" modified="2025-10-08" version="1">MDFELAPDDAMPEAPAPRGKAYVRDRYKIPGWNNPPGNLVVVYLEACERHKVPANSAVIAQLQRRVRLMQPGPWQRITPPEQSSSAPPLAAASGTASPPGGASRLSPSRSSSPTVSYAPSPSPSHSALPSNNPSGYIHSPSQPLSAISSHMAVAAAAQTSQELRDLIQAHVYPLTLSPAMRPGTTGGTRPSSENHAETVTAAALALAVGPPNGQMYSAKAVAHVCQLVDPRTTWEQQLGSAGLPPNSRIISEEDFSRLLEAAAASASLGELRQAALEVLSRPVGHGTLDLEGLAGALFDYLDVSGQGSIPSSELRAAVSAFSPAAAADLYGLMERFPSLARPNAELVSRKECVEVLVALAPSLAPPGSPVGLVRDKVEIAINALLDSKIREPFGYDFSRCYLGPRGLLPVLDALSFDSSFTQLSLANCGLGCSAVEVLVDWLQGHPSLTSLDLSNSLIADRGGLALVRLLQTNPGIVELGIGSTHIIPSRPSRVHHDSLGPSPCEDGAPLLEALAANRAARRSSAAEIVRALRQHGPELRAMCYSLLARDGSVSSSSSSRPGSLPLPPAAAAAAVRCATPPGGPPDGRVSMTALRAALREVTSEWGFTADALDEVLHHERLLGTATAGRTADDSGRLGVTYDELMQFLRSEDQVVRVAQAFRNHLVEAKQIFFALAPEPATASSISVAGVAGPRTALSTVRQAIVAAASQPGSGWDVSEGDFEAVLSPAFMRAHCRAAAAGTARPLALASGGAPVGLPAALRPDGLLVPEWSVAPAPGGGSESAFTITLPMAAALHAGDMLISWPELANTLLYLFNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV42</accession>
    <name>A0ABQ5RV42_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="267" mass="28224" checksum="6E39E96C22DD6788" modified="2025-10-08" version="1">MAHSCRAPSHRGFACRSSHGRKVVLVRAQTDTPKAPAVVSLMTTAALVLTAVPALSPPELMLLRSARADDTSEDLMTPYQRRQRELEKRRELLRQAREQAEGRAGGEVAAPSTAEADAAVEAANAERRAAASQLASELKNSATAYESSTATSRYRGFGGFNAAPAPEPAPAPAPTPAPAPKREVEVKQAATVVVKEVPKVQPRPQKRRGPLPLFLAELLVLGSFAGAVLAVTKYSEQTSKAFALAGAKGKELYAAAEDALAKAQQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVH5</accession>
    <name>A0ABQ5RVH5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_004049</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the thioredoxin family. Plant CITRX-type subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044182">
        <property type="entry name" value="CITRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47834">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN CITRX, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47834:SF2">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN CITRX, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="90"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI61630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="241" mass="26028" checksum="FF9A367E14A9BEA0" modified="2025-10-08" version="1">MSTVLKRQPASIISCPGARPRKYCMVLKVASAIPSNTSSKAVSTVDAALRKRTGSFVNFSATAVDFYRVMARLEAASSLPPETLSGMSPAVPILPAPQRKAWSGILEGMFGRSTSRNQEHIETPDTTSDFDNDSPLTHAVVMFTATWCGPCSVVYRELKLAAGRLQDRPPTAIIVVDVDEEKELASQLGIKALPTLLYLSPDESRGPIFSQGPVSASFILDALDRAAVFGGRDVMAKWRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW76</accession>
    <name>A0ABQ5RW76_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAP30-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012479">
        <property type="entry name" value="SAP30BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13464:SF0">
        <property type="entry name" value="SAP30-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13464">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN HCNGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07818">
        <property type="entry name" value="HCNGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="28611" checksum="B811AE5971746AB1" modified="2025-10-08" version="1">MKRKHEAVGALGLGGYASDDEEEDGSQDHGPEDSMEDKLENVDDKRIFLPNYLSRESLDLNEVESLEAGSLDDQRRQCPDGLETSGAEVDGGKGGASGRLEPDNPLSRLPPELRDPLPTTCSEELQARFAQYLDKLRTKGISLTDSITHRKDFRNPDFLQKLVDHFHVQEHGSAFPPEVFDPESLPTEDTIDQLIKALEREQERRARLREQQSAAGTARIDFTKGSGAFGHIPASNSASNLAMAQAYAQAAVARVVGTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWG2</accession>
    <name>A0ABQ5RWG2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="1">
        <location>
            <begin position="52"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="163" mass="17624" checksum="F39D1EFE962A8482" modified="2025-10-08" version="1">MQLSKFSSTQNVRCPRRRCPNARRPGYLHVTAVFTLMDGQGQSMTYNKAQSIIGSSSSCDVKVAGPKVAEQHAAIIQKGKQTFLKPLVGEDMLDDSRSWKDGVALRPGVSYVLASGCRLAFGTPETCFTIGFEEGTGSNPLVEMMMKGMLASSSPEVKRALDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWP2</accession>
    <name>A0ABQ5RWP2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="142" mass="16153" checksum="8E50F70F7CD41CC1" modified="2025-10-08" version="1">MLARGNAGCYVSSRVSRKGVLVQAAADNQLLKRPDLKRPESEPKRLFSDAASLGAPNDPVRITVEYQRQRAKEVRQYFLDQRVEEIRERSKAFGWTPKNEISNGRWVMFGLLVGMLTEYATGVDFIDQLKLFVSYLGILDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX33</accession>
    <name>A0ABQ5RX33_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004710</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF140">
        <property type="entry name" value="ZRT (ZRT), IRT- (IRT-) LIKE PROTEIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="424" mass="43468" checksum="C36B1841C12211DA" modified="2025-10-08" version="1">MVRRSYTAESQERFWFDILSAIVLFMTAIGGAWLPKYLCSHGTHLSSSKSLAFHLGNMLSAGVMLSAGFCHLLADAHRSLLFLGRFPISNFLAATGYIITLLADQVVQFVSEQQASMANKGDRSNAGAAGTGASGGGDTPSGPILRNGGYHKLPSGAEMTSIVASDKVVTTPEGAITTAVQRESSLISKTGADPELALVASVADAHGHFSGGLANGRSQNGLSCGNGSVQSRGHSNGGKDDGDCAAGSIRALNRSQSTGGDCHSAGVVVLLGGGGRRLSFATAVLLAAALCIHSVLEGMALGAQSSMRSTEDIMVAIAAHKGLAAYALGASIMESGASSERFWTVIGCFSLATPLGIFAGYGMSTRSANGTSGAALSALASGTFLYVAFMEVIPKELADGKHRMAKMAAFLLGYSLMSLLAVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXU5</accession>
    <name>A0ABQ5RXU5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methionyl-tRNA formyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138:SF5">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="1">
        <location>
            <begin position="60"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl transferase C-terminal" evidence="2">
        <location>
            <begin position="304"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="58759" checksum="98E32453F8080F43" modified="2025-10-08" version="1" fragment="single">MAKGATGISRLAVAFAFARRKWQQPLTSPLGGVLNFSTCAGGGERGRIIFLGTPQVAALVLQELLQAAAAPDSNFEVAAVVSRPSGRQWLEKERERQARAAAMTATAAVAVAGGAVGTSTAQTTSAVNSGQKFPTQMASLDSQILQQHNQKQQARLPNWSRHGPSAVEQLARSRGVAANSILCPVSAKEAFLDIPKYGTLNIHPSLLPRYRGPAPVTRALQDGCSVSGVSLVFTVLQCDAGPILEQTQVPIDPDIQAPELTEHMFKLGTQMLLRQLPRLLSEAVTMRDAVPQDEALASYAHKVGKLDGLLDFRQSAQNCHDLVRAMAGWPGSRASLLVENKTTGSLEPIEVSVLRTRVVATLPLPSGHAGPKPAAISTTSIPGSTAAANTMSISSKLSGQLPAGTRGQLMYTSTGELFVPCGDGNMLSILEVRWGYWRDVGHLVKSYGGKLHLGTVEGSTVAGYVCGADGTKMRVLIPFPWHNDKRMSQERRPFRRSLQCERFHILRDMSFVLPLPYPNCLTTPFSRVPIPHRPLIRGHGFNPASLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXV1</accession>
    <name>A0ABQ5RXV1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004481</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039267">
        <property type="entry name" value="Lsm11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21415">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21415:SF1">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="2">
        <location>
            <begin position="200"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="421" mass="45006" checksum="0F3351989FB402BB" modified="2025-10-08" version="1">MHLRDMDKDPSQTFQQPHTAHTSPQLPPPPPPLLPPPLPRTAAASPHNAADAARPAIHPPTHTESSEEGEVTWAAEITGLEAAATTAEVAVAGSAPPSPSLDFLSPAFDSLRALLTPGLQPPLPHVRPMDNVSQCRKLLPPEVPDSLPLEKKPKVLNEETRKKHLRFKESAVRLRERIRSGTTHSLDRVLAAAAAASPGPLDVLRRWHAAAARVSVTTRHATGVRGRATGVLTAYDRFMNLVLRDVNERYTVPVRRVKEYTRLVPVAAGAAAGDGGPGSEGSDAVSAAPGSVSAPSPLRMGEVTRIRIVQRREVRQRQLGQVFIKGDNVVVVHLAPVEDGEGGAADLTALPGSALKATAAPAATCPRLCCRVEGREAPRAAWHPNVRDSSPAPWALTMRLTNRRAMRDSSSRCSQLSGQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXZ9</accession>
    <name>A0ABQ5RXZ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004679</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040626">
        <property type="entry name" value="Pepdidase_M14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF45">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE NNA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18027">
        <property type="entry name" value="Pepdidase_M14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="5">
        <location>
            <begin position="519"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="242"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="851"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1011"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1084"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1204"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="321"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="851"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="875"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1030"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1215"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="776"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1255" mass="132122" checksum="98B64E742986149A" modified="2025-10-08" version="1" fragment="single">MESHSGRTSSKLNMDEGGRHNMLSALPPPPMPYGVPYFNQSSINNSPYGHLAPLLRKPDAASLQPLPPAPPPPQQPQPRPQPPLDITRTPLDNVRNPSPLPLDPVRPPQPLPQPEPPRRTVQTYRKRQHEATESPFDFGAWHVPGMLAHPEGDIPNLPPRSGTGMRLRPARLPPCSCIPPSYPEPLVPVDPMLPQVLRMHDQRRLVNPSQVANVVVFDCIERPPSSSEPLVPAAVEGAAAAGTTIGPPGAEGEAPAAAGGSSVGTATSGEGSGSGSGSSGQATGMGTGGESAVDAASGAPGGRVSGEGSQPRCPADSDGSRATAGGVTAEAGGAGEASSAGAGEAAAAASRGAAGSASCSYTRTTLYDYFEDMPAWYTPRGTSDDTLVFESRYESGNLRRAVQVYPYEYDLILRPDINTRGHTQWFFFSVTNTRAGCRYKFNIINLLKEDSLYNDGMRPLVHSTKAQAGRGLGWHRAGSRISYYANTIRRGRSGKTYYTFTFTLTTEFDNDLVHVAHCYPYTYTDLQRYIRSLELDPLRRYHMRRETLATSLAGNAVDVLTITSPTDDPEALKRRKGVVVSARVHPGESNASWMMKGVLDFLMGPSLDARILRDAFVFKIIPMLNPDGVITGNYRCSLAGVDLNRVWNDPSRKLHPVIHATKLYLKQLLDEREVVMFCDLHGHSRKRGVFMYGCEKKMPRDQNPAFPGWPQPGSVGGQTSIPWRFQEKLLPLLIQYNAPDLFSYQDCSFKVQKSKSGTSRVVGFRELGLINSFTVEASFAGPISGRLARQHFTTNHLEHQGAALMMALLDYWDPDAYGLGDLLGQLDFMHPANGEAPPKFITTVDGQLLEMEDDEPPTDSDDESSDDGTRGGAAGPRTRIATGGRTVQLGPGSLGPLARDAQGASFMQQYYSAYGMANGGAGSNGGGISGGGGALLSDLAGGMQLRGPGPGGPGDKVHSDSEMVMDPKKAKRRARLALQRAQLKAEQAKATEGLAACLPMMAALQVQSPTSSFAAEGPGNRALVGGSGGNDSRSNSFHTGSSLRPGNGPGGGRGASTPATPHAIEYAKQRGVYRGDAPQWTVPGLNAAYDMPEGSASGPPSPSPQAHRPQSPHRGTSNGPLSGAGLGRLSTSTNAFNALLYSQPTASGSMTSAATAGGPGGSILSTSPGVFATSTGTRGTVSPLPLRNSGYILTSYGSMAALAREDSGGSGTGVDPGVSPSGSSISGRLLNPLPTNTVRAAAAGSSGSVALGASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYW3</accession>
    <name>A0ABQ5RYW3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_005515</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040072">
        <property type="entry name" value="Methyltransferase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048641">
        <property type="entry name" value="RlmN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544">
        <property type="entry name" value="23S RRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544:SF9">
        <property type="entry name" value="RADICAL SAM SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21016">
        <property type="entry name" value="RlmN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="232"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="647" mass="67926" checksum="D877B0B939751D29" modified="2025-10-08" version="1" fragment="single">LVRQHARSPRVPSASALREIVDEINRSNTHESDGRLSVKSLTLHELQQWFVAQGERPSRADQIWRWLYGNSGSRRSVVTSPKPSIRESSKSSPASGSGLNLERRVSNGCGDAAADGERGFGAPAGRPGFEGVCGLLTGTAGPTGIMYFGGGLRCLTDAAEPAVDGFSAAFVTRFSSRLSFNGGLQLEEVTPSLDGTRKLVFRVTSGAAAGGRIETVLIPWYRDYDPRVGRQEQPRYTLCVSSQVGCAMNCQFCFTGRQGLLGNLTTAQIIEQVLVAQQLLEDAAIRRPPATAPSPGPAGPGAGPLLAAAPITNLVFMGMGEPLHNCPAVFAALDILTHRRGLCMSASRITLSTVGLLPQLQHFLDQYGPPAAAAAHFTSFTPSAPPSVPAPGMCSSRQLQTPRVDMGSAVAVAEEEGAAATTSSSASTTSPRISLAVSIHAGTDQLRAALVPSNRRLMLSTTPSSSDIVHGVVAAAAEVANMFPLEDATGDGGGGGGDGTLRGLSALCAVLSRHYPRTDRRPLKAGRYVLFEYTMLGGVNDRVEDAAALLRVTRHIECSFNLIMFNPFPGTMYVPSTPERVREFLGVLRSGGRIVHVRHSKGDSSMAACGQLGDVGPRGTGRVAAKILPEAPALAAAVTASVVAKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYY8</accession>
    <name>A0ABQ5RYY8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="139" mass="15576" checksum="798201DDA7AF1D45" modified="2025-10-08" version="1">MAMIFSPSPTRCLGSTQRHELRLGQQPTHDTVPWRFWLSSQREATRQCHLISPFTRIPVRQKWILLAGAMDAMPTWTLDQIAGLVFGAVMIAAILSARQVDVMVAKAQRRQLGLCEECGGVFEPGNCKQQNCPAKRKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ21</accession>
    <name>A0ABQ5RZ21_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MTM0417</name>
        <name evidence="9" type="ORF">VaNZ11_005639</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="8">
        <location>
            <begin position="42"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="239"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="280"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="437" mass="45441" checksum="27864431D8F5C2F8" modified="2025-10-08" version="1">MSARQGFTGIGESRPGIGSRTVTRNLSTSGERNENAMMSDEIRSEVRRIVPSLTDDRFKGQSGKIAVLGGCFEYTGAPYFAAFAALAVGADLSHVFCSTSAAPIIKQYSPDILVHPYFLQRSDLLQQVNQPLPSGALGGLSTNHLIPAEQADVAAQTAAAAVHVAEATAEVESWFNRLDCLVVGPGLGRDPLLLDIARSVILRAKAAKMPLVLDGDGLFLVAREPELVVGYTNCVLTPNLNEFRRLASTMGVSLHGPNNDRSSKLLEVTAHLRGPTLVSKGPVDAICDGKVTMICNASGGAKRCGSQGDILAGTIATFISWTLTFLDIARQSAEVEVVILPEINPMVLASYGACLVTRTAAAYAFAARKRAMVASDMLAQLGSAMEMLFDTAGGTGAAGAAGPGVPGQGLLQAHVPAPNASISVGCPVGVLELPGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ73</accession>
    <name>A0ABQ5RZ73_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_004981</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH 2/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065:SF3">
        <property type="entry name" value="FI20425P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="10">
        <location>
            <begin position="69"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI62362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="337" mass="36290" checksum="A10A93E3730BF572" modified="2025-10-08" version="1" fragment="single">KKVSESTTATTGRFELPFQDSLSCCSVLDTCLGVASIILVFPIVQRVVPFYFAALNTVPDFGMDDSGESSCSETHQCRICWGEAGDPSLGLALVSPCKCAGTLNYMHVKCLEDWQQVLRSQGQYRKARHCEICRQPYELTTASSCGGSSGGSNGRWSYVSRLLSRLSHQVLDVLNCCRSYTDLLYRCWKLYILGSSMVGAARAGVAGLRAGFGMGRALVEEQTGILLSLLSAMGEHFGTPYAELLWCQAVACVAFALVSELFYTSLLGMLAGGVIGFFRGYLSAVYASVSVVVRGVTQSWSLGRGLAKAAGLALRLPWGALRAVTALVAKRLPVRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZJ1</accession>
    <name>A0ABQ5RZJ1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="15309" checksum="7D65876CFF82D2FF" modified="2025-10-08" version="1" fragment="single">QLMPCVGGGSSSAAVEGRSSLLAVAEAARRRESVSSIPTIGSVEEDWHDARSVASTFSIMSCFNTSAAVNSTTTLHPHEGDGAERHHSDLQHSPVTHVHSCQHQPCAQGAVTAKVVAEVLANNTQEMRQQGGAGDAPSAAAAAAAEAAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZT1</accession>
    <name>A0ABQ5RZT1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018790">
        <property type="entry name" value="DUF2358"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31094">
        <property type="entry name" value="RIKEN CDNA 2310061I04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31094:SF2">
        <property type="entry name" value="RIKEN CDNA 2310061I04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10184">
        <property type="entry name" value="DUF2358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="40993" checksum="3F1B204D93C86C6B" modified="2025-10-08" version="1">MLFGLRGSNLPQGRPGVAGGAGVRGVVSALKQQPGELRGEPLPQRLASKYEPCEPSTSSLADFGARVTLERLYLWNAVDSALGGMPHGTFLVTVDDRPQRTRNQDRSEDYFANVGDAIRCLREDIPMLFQRELNYSIYRDDVVFRDPRNCFKGMKNYQLIFWSLRFHGKIFFKTLYVDVKRIWQPEDGIIKMRWTVHGIPRVPWEAEGTFDGISTYRLDSHGKIYEHSVDNILLRDPPMATNPPLLAGLNLQPLAPQQPVPGAWCKGAEQAQQHWGAYVYVQRMVASALQRLESDYREAEPAAAVAGISASPPPLSAHAAAAAHTVYPRLSTAGAGGCASAANGASSGAPWGASGGSGSGGALGTEDGDVWGRSVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S016</accession>
    <name>A0ABQ5S016_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_005854</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052201">
        <property type="entry name" value="LRR-containing_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24111:SF0">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24111">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="349"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="464"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="768"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="824"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1070"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1134"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="492"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="824"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="856"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="871"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="930"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="952"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1070"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1087"/>
            <end position="1102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1306" mass="134519" checksum="B4988E32C9365A83" modified="2025-10-08" version="1">MDRLLEVLTGLAPLPPTRFVGHEGSIDDDSWTHLLEALRHNREAVGVRLRGCGLSCTRASQLAEAMVTNTGLEVLELPDNNIGELGVQALVDALRLSNCTLKSIDLSGNPCYENAAHVLQELDSLLKRNRVIKKLPKTPTVGSNLPMCGGLASISVHHTPANALPTPQLAQAFLATGLTSNGFSFELEQRLQHLEEAEATIRSELEHVRSFSSQSEVWQKKIDAAGQQIAAILSLVDSRNLTLEARLQTMEHWRASAEQAWAGQSKVLEDMAGQLLGLMRRVQALEAQLGRTDVFNGSAVSVRSSAGPVNLDAALSSAETLNAVKIWQEMTALRERLLKLEDESANAARTKVSLGERPPCTAVEQQQPQEQQQEVRAVESTPANAATQQGPRPVTGNNGDDSIYSRGQQHAILGGGKPACKSTGAPSSAETGHREQLTRSASASELSSVDNLSPALCTHDLDATGSTATGLSPSPAPMGKKHGGSSKPWWKKQQSQTARTTGSLQDRRGVGVKPLQVPNSPILWHVNTFAVSPGSCAITPTRERKSLFETGKVQPLPAGAVLLSNCSHGCIDMPDIVDEQADPGECSGFVMYAARPARMREGMAQTTCSSAAQRYAPFQSDPRDSVDMSTHLRAPAMNGGLEEVSASASAAAAPYHEVPMLWSAVTPGRPGPAHDSPGTPRGSTFSVDADTVSLSVIMAGSAARPRAPAVATTDFSPLAYAISTKDPVEPEADWSTGRPRSDKVKQSLLGAVPELGSVALAASSCSIHAAKDGNGPDPTEVLGTREAPPRSPRTEAGVEVQAALDMIDAPGVTVREWAASVSAGPSAPAAPAASTGNSTPTSKPCPATSPTPSASNARQGQQQSMSPPLGSVAARRTAAPRASRSGADSHHRSTVPDTKVAGGASAAGAVLSPGRYEGHSNASRPPHAPPHQQAASVASTGAAGVAISVSSRPRDPSGHEREHGVHPRAASSSIVSAREYALPLATEDVAGAGSVGSACAGLKGNASLQFMRSGTCTAPGGHPPHPAGPHTAAAVYTGHSAGHVSGPGRAILPAHSARSFSSHRGEIGGGASAKFGGAGPGNRITKSGVSSLHRSSTSTNSTAVTGAMCGASMNTNRSAISVAAPTIAAAIADQSLRHGSSHRARRTEGGASMDAGPTSGHSLAAGPPEAEDSQGHDAPSTGPLVAASVQAVDIVARKVSQSDGGVNGGNGHDAGHGLREICKEHSTCSLDTGEAAAVAQAQSATDGSAATVFQQPQHSSRSYRPGVRTLVPGAAANVAAPHLKGGPARTSIRNVTSVAGATKRWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0A4</accession>
    <name>A0ABQ5S0A4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006192</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05167">
        <property type="entry name" value="PI4Kc_III_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045495">
        <property type="entry name" value="PI4K_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19274">
        <property type="entry name" value="PI4K_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="7">
        <location>
            <begin position="1694"/>
            <end position="1897"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="6">
        <location>
            <begin position="1983"/>
            <end position="2263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1826"/>
            <end position="1849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1879"/>
            <end position="1903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1879"/>
            <end position="1898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2279" mass="241325" checksum="B7DF34EA02FCA3CE" modified="2025-10-08" version="1">MSMISLLELRSSWAQSLASINPVDEEKVALLLRLCAQQPVSDEKNVSLGSSSARNVATNILPLVGFIDKSGGKRVDAIMPEVIRQLPLLATRWTEPCADSSALAHLFEEMFTILHRVLYNKDVPVKLRKALSGVVGDLLAQLVKTLLHMPTDVPAGAAAAAAGGGALVQPTAPAQQQQQQPAPLPGTSPAQLIMRALTRSQAFAVGAGVPLLPAEAKVLLSWMVENVLPAGGKPASSSAMAPAPGTRAGVGAAVGGDEKLLLLMAQLVRTMLMGQRDSTAGDGATALGGVGSANLQENERQLLTTVLTWSMGLVRGVMTKLLQPVTGLEVLSSTLLAGKQLPAAPAQPQTAGAIRAAARLAATAMHGLLLRSVGTAAVGGPSPAGVLTPAARTQLMDVISMLLDMSESCVSLAMVVGSKMAGADKVLDMAAYILEDCSSLIITGAREAATFSTASAAPLTNGTAGPKLHPSTPAAVTSLDVGTIFSRLKAILLAAAALVDYESGTDAKANWRNYWSWDVSRPLTASYVRGSRMPGWGAQRLCCNVAETLVCGLAASYHAGMPTYARALLLLPLSLPDTGLTAVGGNNADAGAGAGVSGRDANEALVLSLAMGRLFVLLRGDEELACSMVPLLTDVLAQPATSPAAVSLQASVVQALAAIAANSVTSERAVTWAYNQITDVLLRLLLEARHPGAAAAALAATAEGPGAEALSNGLLKLAKGIRHAPSVARRDLRNRLLVQFSELGLRAGSFNRDHDSVRAEMGRLLPAIAEACEGLESSALSASRFSMGSLAAQQQLNNGSAAKADLHMIRLLRNLWLYVALFGLSSSKGASPEQLDAAGRIAAWTPVLMLAEAGASETDMTEQLKAELGERLRLAGDALASPARLRSSLQSLLDVPLGGAPQPPPDANLQAYLLAVATAEVCRVKYAPLKADATASPISVALTYLQAADPVSSEAAWYAKIAKKAFEVYLGRLREETSRPSSSTATTASPTDDGIAAGVAAEEDHRRASSYPSGRNPSYAGLSFIARNRDANGVPNSVVADLAWPGEATNNTGGAASAAPVVVRDIDGGDDAANGRAASERASGLARVSERCLEILAVSLIRHLGSAGGHEEGVAPQVATVSGLLLRDLLDTYQPVYWSYACMEALLSELDSEESLDHPLSEVHGANNLSAQAAQGTASMVLAPGMGGTVWRWLHTWVLEAARIAPARTEALLRLFLDETSISSHALEGDAAEGAVGALDGLAPPLSLSSSANSRARAAALRRDNDLMVVTAEARRQAPVGAGAGGAGSGTDALRKKLFFSGVIAGVQHITGGHAAGPGAVAELTLSGLEEGLAAARHNFPLLRQRYLGAAAFLARRAAEDGEEESLAGSVVHSVCYRVLQQLCMAPLRRFRPEMMYLATFAWTWISTAGPSWLVPLVSRAAAAWSATVDRGMGLFDGAWGKKVIAHNHAASSVAAAEDVHIADDDGAAEWELLKGLSCHHQWIAFLYEMWASAGDRTDAERRALTSVYDRLLHHSLLAPEERLCSHPAAVAPLFRLLQLTLSYLERASRSRPWGPPQALLFDRVLRAGLIWFSHPVAFTARISQGEAEEQHRAVADFVTILEPMLKNPPPAAPVAGPVYSAKRHGRCAMDPAAWSSRPSVSEAVWGPAAKGLRMSDAAALLLLLCRAEIARLREWSRPRMGPTVAPGAPPLTAAHVQTAWKVSPQLAVAILQRFPTPLNGGASEAQRALTELVLAAAADPRVQALPDAAMVLATDEAARARKPQLAALATWAPASAVQGLQLMCGPAQHHKGIKAYALRCLRAASPAKVVFFLPQLVQALRSDGDVDNARSWGGGGHKGKGADEDTQDGATGQYLLATAARDDKFAHQLMWALATEEKPPPEEFTPEIKRSGWEPPKDTGLWDPAEKLKRKVLAQMTPEQRAYWEAEDGYFNKVTSISGILKKLEPDARRAKIKRELEAFGPTRADLYVPTNPDCDVLGHIPESGAPMQSAAKVPILVKFKVAHNQPPPLPPLNREVACIFKVGDDCRQDVLALQAITILRDAFQKAGLELYLRPYGCIPTGYGRGIIEVVPKTQSRAAMGELAERGLYEIFVSQFGPPNGDRFKAAQRNFITSEAGYAVASYLLQAKDRHNGNLLIDSEGHLVHIDFGFIMDISPGHNMGFESAAFKMSHEMTQLLDPGGKRNSESYMRFQEAVVRGYLVARTVAEPIIASVALMAESGLPCFGYGKPLVNLRKRFHLEMTDAQASAYMRMLISDSYDKWTTGFYDFIQNMQNRIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Z4</accession>
    <name>A0ABQ5S0Z4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal RNA-processing protein 12-like conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006530</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012978">
        <property type="entry name" value="HEAT_RRP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057860">
        <property type="entry name" value="HEAT_RRP12_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052087">
        <property type="entry name" value="RRP12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25772">
        <property type="entry name" value="HEAT_RRP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08161">
        <property type="entry name" value="RRP12_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRP12 N-terminal HEAT" evidence="6">
        <location>
            <begin position="9"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="RRP12 HEAT" evidence="5">
        <location>
            <begin position="358"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="658"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="971"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1140"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1266"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1365"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1432"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="980"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1168"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1365"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1387"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1397"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1451"/>
            <end position="1468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1485" mass="152445" checksum="6A24794AC080F038" modified="2025-10-08" version="1">MASDYGGDADGGVATEVAQRYGRSAQPESQQVVAVLRAITDVIRNEGLSPSPSSYFAAIMSALEQPSTQGSQQAVLAMLTLLAVVMPRLPNHVLRSRFTSSATLIGTLLAAYAGEAAMAKAALGCLAQVLAAADPTAWLAAVPAFNQLLNFTTDARPKVRKRAMEGLVETLAGLQRSSALGAASELVLKVCQRVLPGPAAAAQSAAAASNKQRSAAEEAIAAAVADALHMIAALKGALPLLAPQTAKAVSELLVKLYSLRQPLLDRHTTEALSALAGAGGRGEDAITRLAPGDLAGLLTVVIETESLWVSSGRDAGLLSALGRMLEGGLAKLAEVDPAACAARLPRTVHALVPQLAAEQDGVRYGTFEVLRAIITDCLTPDVINAARMTRGSAVGAGAGPTALASLVAAVASALSAQYQDAWGMALPVSGALIEKLGELGAQDAAAPLLERLGAIVAGSEDAAAAADIPDSGAGDDDGRGPATAAYGNAAEATLGSAIGALGPAAVLQVLPLQIKEGLAGTAEPRTWLLPLLRRHVRHAPLGFWFKEMLPLAQDLGARAAAFSAAGGTAAATMALKCRTLELQIWHCLPAFMSWATDLPNVYDTVVKTLAAAFNNRPDLHPPICQALQRACAQVRHVLVTEGRGPDLVGYADPTAHGPSGAAAAGGHGGGGDEDDDDGAATAAATAAEDPAVAAVHGGAPPPGYGTAEAEAALAALRAFNKQWLSVLCKAFYNSEIGTSGPYAAAIAAMSAVTDTAVVGGYFRAALQKLSKIIMDEAAEVPPADIVTEGGTDPSARRNTFLELALWLSYGLPDAAVDVLFKATKTAIAHIQSSVQKRGFKVLAVLAEAREPWLRANLGEVLKQLLGSDAAATISAARRYRLRLIRPVLVAMQQPGVELPPGILRQEGEDEAMAGAEGDEGAGTLSDVGQKKELAAQLVAEIVLGTKEANKRTRSAAYDLLVGLAHVMDELDPGQLTGSDGENEEDAMEEGDGGSAGKGGLYEFFTMVMGGLAAATPHMVSATVMALARLTYEFAAAMQNLVASLLPPVLSLLRSKAREVVKAVLGYVKVCAMRLPVEILLPHLGPILEGMLVWAEDSKNKFKLKVRVIVERLARRCGFDAVAAAMPAADSKLLAHIRKEVAKRQRRKHRGGSEAGSEAGEGDNRSHRSGATSGRSSLARTARASHWGHTAVFSSEDGDSEGKGAPGGFGAKSVRSGAADRGGAASLAAQKAPGRGGGVRLAGGSGDPMDLLDVGASRQLARSAAGVRTSVDAGGSGRGDDFNRDPGTGKLVINEDEDDGGGRGGNKKRRHTGMGVDGYDSDDSDFDDLRGVADLKYALKKAGDSRSVRFAEAAKSVVGARSIGGRSVGAASAGGRSAGGRSTASGASGKGAGGKGAHSGDRFRAKKAAGDVRGKAKVEPYAYWSLDRRMLNRRRGKQAAASDKLASIVSGAREGAAKGAKAKRQAAAAREAKRQRTGPPAADVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S162</accession>
    <name>A0ABQ5S162_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="426" mass="43882" checksum="E34D40AC518270F0" modified="2025-10-08" version="1">MQRSCLLHPNGSSKLTTSALSGKRACYCRVLRRSCVTAATRSDWRAEIEGSDSGSDKSATTTTTATLQSPAEPRPAASAPPPAAAVPPSPWSQSGPSSRAPQQQPAATAAGAAAGAAAAAPTATPKPEDAKREEPLSLPAFDPTALFSSPKNQASAFTALAATSAILSVLCVVEPQAILHVALPEAYTSDLDVTFLRTAGVTLAASAAVEYSLKHAAESQLLKSATYQRLMAGCALKSVAFLAVLAANTPSTLQLWSLPVWLAYVGTAVVAGAINLSVISNTSGKGLAPPPLDLNVPQNPTSWGYATCTALYLLTVLACFAPETLYTGDSYSAVTPLLKGVWAPGFLLAAVMSFVLKDASDRGRLGASTFKNLNLGLAALEYGYSIIFGSAILSDQVDIDLPAVSNLGGSLLIATFALYTYATAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1L5</accession>
    <name>A0ABQ5S1L5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006663</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045945505" evidence="2">
        <location>
            <begin position="28"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="550" mass="57358" checksum="62D4E32633EC8DBB" modified="2025-10-08" version="1">MMVEVLHDSAWWTVLLVGVTGPPEAVAAAGRGTTMAAHGDDGQTGIASGVILHGRLLDGKVAVSVPLELSRAVAVWDPNMGSWSAPEPHSFLPARPHHFCDVMLLEGGSTAAAVLEPLLVNRVEAHKEAAEGARTELEHAEEGASWAAAVEAAGEEMLRAFSLEFTLLQLDGAWLWGVVRERLAALQGASHPEQLTPPRWAAASAATTARVGSEGCGNLGQGHRRRSHGVPQEGSTLGGSTGGSRRRTGRGADRERTGSIGVEGNGAVVMGSGKPAGNSGQGDATDLLDFTGIDPGTFRQYDTRPDPNKGGAGLEKAAPGMIAKRFSALQSAADPSMLRVYVPGERLTGTELQLYARAYGEFMHLYGSQLPEGADRRITTAAKQRCRQVLHSSLTLPVGGDAEERGADLTPGAASNPAKGAIATVAGEGRQRGPAKRCRTSTEGGLAEGSAGNLTIKSEPDKDGSREFSPTAAAGMFTASGRRRSAAAAMVGSARKGPGRRSMGRASDVAPVMDFSCIDPDTFRQYDTRPDPNERGVGLEKVAPGMILSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1W2</accession>
    <name>A0ABQ5S1W2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="671"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="765"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="829"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="777"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="874"/>
            <end position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1099" mass="114422" checksum="AA84BA41197E9B73" modified="2025-10-08" version="1">MKPIDGDAGGMGRRCTCPCVCGARKFNDKQPFSLPPELAKALDLPLTNVVGEYSMPDWKKDMYSELRDCIQEAVDYAFVEWKMPDEAKVLLDGSALKGTALDDSDMDLVVRTTYRVPRSVKLKAHQVIKAKLVEKYGSGNVSFTQKRKSAEFVVEAHRRTAAAATIDASDVDDVDDDQSVQDELRVEWKAPAPAADWSRTTVVAPKCTKLYADVLFENATVGLPLANIQTFPGEGIKPVQMMKLFQRKAGSLPPLKGYVIEALVQHVLECGQPSEPNGAPNPLPTKSVSQAFYMTLTLFLVEPSARDLDNRVCHNLAVPSGFLFDRGDFDCWRGRVKPFMRDLIMLATCLSKDTAKRLDADELFRRVLEPCTPIPEPFQRSAPWKAPSAAAATSAAASPGGGGSCSSSQPGGEAHTDEAMPFSELVAKLKPDVEKACVSICPKDEVNPMYDLGPWEPDVFLWGLETGPQSTHGSVAKPGPNVAVSEGPEAPAPAPAPPSPPSPPFNSQLSSLSPADLDSQSSFAVRGFAVDPAGAAIAGPSGAQGSPPRSTRRSFTSEELLAAINIISPPPQIAQQRQNYDGTFVTVSNVSAGPCVDVDVVRSPNSSISSGSGGASSFAASASLSCSGAIDHPLASLFEHPLAEEARRLCYMYRYPNNPQVGLLPPVPGAMAQPQPQPQPNGCSGQLHPRLAPSKDPPGLYQAVERAALATSSPRPALRPASWKETDIAGSTAEPAMHALGAPASSHDPETGFIFSLSSSIRESLSRSKPGCGGSSGNSSNSSSSSGGSVNTNNCDEICGAAGSGSLAPWPRSTDRSAIGRSGLAAEIHAPSSATGEIPGATSDCDPRDPAPAAPYCSGSPIRAGIGRTLGNSGKNGDGGRGGKYGGSDSSDEGSNGGGRGAVPPELETPAATVQVEWAQVAAPGLPDQSARRWTDSPSIPVSTLRSAAATPTPTAAAAAAATAAAAIDTVAALTVTHLHPMHIPLCAHAEVAAAEAEAATAPELDALMQPSTCVHNATAELLRSKVIPRLLERLQALLRDAQERRIQRPLTAVAADDESGDNTPTTANAAAATTAANAANAAAVAAASDAIVRERGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S275</accession>
    <name>A0ABQ5S275_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_006598</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041246">
        <property type="entry name" value="Bact_MG10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13062:SF12">
        <property type="entry name" value="ALPHA-2-MACROGLOBULIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13062">
        <property type="entry name" value="COLLAGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17973">
        <property type="entry name" value="bMG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="2192"/>
            <end position="2225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1360"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="2196"/>
            <end position="2206"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="2215"/>
            <end position="2224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI63593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2230" mass="237273" checksum="C1BE276B5685447E" modified="2025-10-08" version="1">MSRPGPKHCVKYSKMVQNGLIQTNVALSMLLMLAILPHADVVLSARAARKLTTTGATATRSIMGSLPLNITVTSPVGLALGWNAWVSNLQDITVVFSRPVIALGQDFENDGNVKKVVPFRLSCNWPGKLRWVTTSIARYDPSEPWPSNLQCFVLWNTALKTFDGVPLQLGSIPVKSRLYGPALYVSGTSVLSDLAENVTAGQWYSGALLADDKAPEVPPDGHIQVFFSDKVHLQAVASSLQLLPGHMPDANLTEPNPKLRFTVRPCTSPAWWASPQGSSLNINTTCADVEVGPSLKPDTWYTLRLPSGTKYNVVSGPLDRNLDLSFAGLRRFRIPFSDPVPENATAEPRLTVTLRRWDMSLPHGLAAGITSAKLGSVFRVCERVPPTKANPVGGCTPVPFNLTLVGPTIARIAVAGAQPAKAYRIYVSGSSAIRDGFGMPLEASSSEIGMAYVPYAFASPSLLSSMLAVLEPEVVDPLSQWPYITRAPSPKEYGWPAGGYPVNISTFNIDVTNQSDVLGLLATMELLDGWMATNILKDDRLGKPDLVTTVNKNLTTPGTGAGWTKISTPLGSSILRLIYHCCIPRSAYYAESTGKVVLRTDLSLSVVKSGQYLVAWVTDAVQGGGAGPVAAAQVYFYSTSYNYYSSIDAKLQASCTTNTNGYCTVNITSETLYLKLSALAVVTSSNGVRSALLPNSGFASAQPILYPYTFSAVLDRRIVEPGDSLKITGFVQTTSLKGLSPPNNSYVVLSVTPDFNPSVSEPSVKALVPVSNIVGSFHSEIKVPAYARMDNYQLKIWILTNRTSAPDPTDNTIIHGAEFLGDASFTVSSPRLPTAELEVVADPWVQPNGTIKVNVTAVSYLGASVGGSPVTVAWSVSSVSTAHGVLQLTTDSLGFAAGSIDLAPVAAAVALQGLTLSGVVNLGFEWIGPTRERITKSLTVTIADAAVQISFITSIQTDIPNIPFTVVATLTSSADGSLINDVPITAVLSPKAGATCNTTYSCTISSGAGFFGIRASQSTAPPCRLALPCVGVFDLRVCANVSGSGSSTPSEVCSNRTIGRNVSQWLAGPLSKQVDPVLQLDKPRGYRLGESPRLLFENPYSGARVLAVWGTEFDIRTKAFTQLPDGVPFNDTIGPLGPECIFSCTVTIVLDVPRAGATDLPVLPPSNQLSVATLFDPRAPHSHVMRAKIDIVPDNELPVSVSVGPVAGAGNLSTVTDSSGAVLPVVEPGATAQISVNVGSTALDGPVEVTVYGVDKAFLDILPYDLPKPQLDVLVRLYQSVDVFTLDAYRIAPGAIRAVFDKLIDRLTKLDPWLPLETSVVPYGNGGRDPVDTSDDDYLAAHTSPITLFNGQYRPDMIGDVNDMGGSSPLSPAAQRRPSPKRGAGASNGEVEKDTAGAPTVTLETVRKAMQFIVTPLFSYSLADSQGRADFNFTAPQNLGAFVIRAYAAAGRTAKYGSAEGEVVVRRRLSLTPSVPKFVRVGDTFEAGVVVTVGSAPATVNVTLQVLNQSKSSLKSVGPISQTVTFSRGDDLQMEVRFNFTARAVGTASLVFAASDSALGGGKDSLQVEIDSEGQQGDVWLATSFALASNASATRSNSSWVEGIDLPAAVPGSGGLTLVAAVGYIPAVLAVYDNLQKTEESRTDPNAQPAMMWVTLPAVLSYYSQSPTAAQAVEVAQAVQDLQDLTSQNFGLQWVLPEKWSGWSQSRTDIYLNTWALFLVNFHTNNLQNVSGPLGSVVRDCYDTWKRALEYQVINDIYAAQVNGYEYDDWNTLAWIRLALGASWDPQPAGFYNASYVSEVVSLSRSLAAYRNKTTSGQFTRETRILLSLVLLSTGQTSPDPQLVNETLLELTSTFRVQGRTAYLAWGPGSPEPASLQEQVLALLLFVRSGTNNQLVPKLATYVANSLSSSGGGGLYYYYAPWTIQSVAVAVLTEYDVLRGSSKPTLDLEAEVNGLTVLEASFRTNKMRPVTNTTAWEDMPAPPRGQLRGLDFQVSGSGEVTVAASLHFVPAALPSFPAFRGISVDLAIQLVDPLTGGVTGPRVSSVPLGSVVMLTLQISTPDNLGAVTLSIMMPGGLEPLDPNIVPDAGSSCIVDWFSGGVLDYFYPRSWWWPICPSRETRPSLVTFSYAALRSGTSYATIKAVAATAGTFVVPPVRVSVDEQPEVMGMTAGGSFRVCAACTSATPAPPPRVPKPCPEDCSQNGVCNLRTGVCVCDDGYTGVDCSSRPMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2C0</accession>
    <name>A0ABQ5S2C0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_006604</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045277264" description="Protein kinase domain-containing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="485"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="534"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="609"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="874"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="564"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="880"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI63598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="898" mass="95499" checksum="4B69CF6E82DFD75B" modified="2025-10-08" version="1">MSRVGFIVLVSVLLVFPSRLCFTATTSPSAANKSNLTLWCRTGEELVAAIANPVVNYAVLVTDVFVTEAHFNPYTKPMVVQRNFTIMGYDPFPASWPVLDLGFVTAWVQVDPGCTFGLTNLALQNYRQGPHFQGPGYDIFIGPTFLRPARQPPSPAATVATAGAATASDLPGPLILQWNSSLIQRTCLPNSIYNGSVPNLPRPGQVPGNQSAVRLPLPANCSSHPGASTMTRCWPAWGVYSDVASKSYQEVSPGQWAPAGIVLHLLEVNYMCNTLMDEECAARLGTVGCFYSMFPRGISSESSSISRGISSGSGGKRDASEKVVIIASTLGCFLLLLTVTVAVAVFLNRHRSDAAGTAMFGACSFPPGVWDTGNCVGRNRAGSWSVPDHLPSPAACVGVCVETVPPGPQQAVDADVAAAAEDVLMPLQQRTTNPCRQRQSHFQPHAGARQSGAQSPGDMALVTSHTPLSPSIDYDVQVMGGELEVDPGVVLGKGNYGRVLQGRYQGERVAVKFINSGLLGDGCFQHPGVPVDVGGKRRTDAAEEPGGRGPGAGVSWKNDDDLDGGTGTGTGTGVGTGTGAGTGTGTGTGSSEVYVSAAPASEDCGALHHDHHYQQQEQQPGDAPRPLSRQHSSELPVWPQRAFVQEVEVLGRCQHPNIVKLLAANMRPPRICLVMELMDTNLERLMYGDLKRPGRQLPVSTVVHIGMQIARALSYLHPTILHRDIKPANVLISAPDSPAPVVKLGDFGLSRLLQTALVTANPEAGTTPYMAPEVFDTNNNTITDRADMYGFGVVMWEMLAGQRPWDGFTHLQIAVSVAVLGMHLPLHNIGRGNCPAKLQSLICSCWEHDPARRPAAAEAYKTLALVQEGLTRLESRQPETEAAAPPTATTTVLLSPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3I5</accession>
    <name>A0ABQ5S3I5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HSA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007739</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044798">
        <property type="entry name" value="EAF1A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46774">
        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN EAF1 A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46774:SF3">
        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN EAF1 A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSA" evidence="5">
        <location>
            <begin position="33"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="389"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="220"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="572"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="808"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="994"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1068"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1190"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1468"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1587"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="934"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="355"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="572"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="654"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="813"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="834"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="994"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1016"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1190"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1213"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1275"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1469"/>
            <end position="1488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1612" mass="164964" checksum="8F1FE378AC5086BA" modified="2025-10-08" version="1">MSATADQKQKEDALLEKASALKAQWKSLAAAFNFPCEEPKRQATHWDYVLREAQWLAEDFMQERLWKQAAAMRLAHDAANYDRTKLKTVQTRVTFECAPEELGRVQGAVAEGADGRDKALPNGGPDSDPTAPSTPDREAELPGLPLCDHLTFTVLASAEELMEAVIRQLEEEDVARLTAYAELVTDYQVAYDAAQYTRSSATLSDGEDMPIGRKFPVAGLKKGTGARPKLAMPAGADDTDSLGEASPAPAHHARAGAAGGGAAARRAGPAGAAALRAAAAATDDDVHPNKRKRKQIRDFEDDDAVVPSGPVNKGLKLKTGAGLKDDKSKKLKSPAKPPGATHAAGSFGAGREGVGAHTGAPKGAAAGGLKHPLLRGTASIQDGGLRGLPWTGADDQVLAAIVTEFSQNWSLVADVMRSANSMQGVYRRPEMCKYRYALLVKAWQQENGEDGAAEAQHNTLSLAIMPKQQAKEVLYQMLPVSDATLRRHQEAVANLHLRFRTKRHAERQQQREMYRTRHEQHTSHYVVSQNVLQANGGRVLNPLELALGPSIPDSAAVLTAAAAAAAMQGGAGHAQATPSLGQQQQSHLAGQQPMAPGMPPQQTQQPSQQQPLQQQPSQQQQQPGMPDAGQAAAAATATGAMQPQPGIPGGLLPGMGQVPPQMQSMQPLQQPGQAGQQQMQPGAMHAGAMHPAQLAAMQQQQQQQQQQQQQAAAMGQNAAAAQAAAAAAMAQQNARMQNAMLQHQQAMQQQQQAAIQQQQQAAMQQQQAIQQQVAMQQQQAMQQQQQAQQQQAAAAAAAAAAQGQPTVGQQALGMAPGAPTGAAPPGQPQPNSMQMAAAAVALQQQQQQGRVPTPPLQSMPSLPGMQSVRASNTAMLTNQQALIQSQMMKQKMMQQQQAQAQAVQQQQQLGLAQGASAGVGAQPTPQQLAAAAVAQQQQQQQQQMAAAAQQAMQQQQQQVAVQQQQHQAAAAAAVQQQQAAAAVAAAAAAAAGQQQQQQTQPGQPQPGQLPGTQQAMPSSAAQGTQQGLQPGNMQGQLQPGLVQSQPQQNGIGNATVAAAAATAAAVQLNKPPTPNPIPGASGAPGPGTQGNAATAAAAAAAAAMQRPPAHPTQLQMQAALAGRLPGMLGAMPGALPGTAAMNTAMNTALAAQMQKAGLQPGVMQNAASFQALQKMQQQIQAAGAAAAAGGAGRSPMMAGGLPNPGTGVQPTIAPGALPGQPGALPVQLAPGQQHGLPGQVPGQQGLQQSQQQQPGLQPGMQQQGQPVGNPGPGQIPGLPAGMGTLPGGLQQTLQQQQQAALASMQQSMVALQQQQQQAAAAAAAAAAGQMNGQGGTASKWPTGLTPQASAQAMAAMQQQLQQQQAHLLPQAAQQAAAAAAAAAGQAQSMGAPGSLPGAQQQQQQQAAVAAAAAAAAAAQGHPNPQHAALVAQHAHLQQQHQHQQAALAQFQVQQQAAAAAAAAAAAAGGAPGQAGPGGLAPGQAGPGGMAASSLPPNMAAFINQNPAALLSMTARPGLQQMPTALQQQLTMQPGPQPGGLQPNLQQQAAAQAIHAAAAAAAAAAQQQQMQLPGGGPGTLAGGMGMVQPGMVQQAPGMQSQQQATATAQPTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4C1</accession>
    <name>A0ABQ5S4C1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_007514</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="242"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="519" mass="55108" checksum="D40F0382BA9664C4" modified="2025-10-08" version="1" fragment="single">GNVAQRLSEDLTRLFARLQLDDGDEWMGAEHLSSCQPVACASEFGPKWSDYWSDPYELSDRASALEHSLRRRSICSAQFSLDADENHSSCTATSSHVKLGSALRALDVSVSSEHTAEGPLGAPRACAGGVSRRLEQLPWGADFLPPYMSAFGETSGEVSRVRGLPPVPSLITLSGDNKSFTSTAPSTAGNPLVLGDSFSPARAPAMGVGLVAGGPGADATHISAVAQCSPLRGVVKVRKTEVSNEEKIGEGAFGQVSRADVFPYGDVAIKWIKPGHIAKHSDAFLVEADILANLNHPNIVRMLGVVTSPPAGDNAGDCNGGAATGVSDPVVGIVTEFVGNGTLGQVVRSPNGRLSTRQCARIALQVAVGMAYLHSQSPAIVHFNLKPDNVLIQGEGENMVAKIADFGLSKPKHGKYVSCPTPRGTLPYMAPEVVLFLSVVLLSPATSAAPAAPPRPLQQQRQQLQHLEDVLTSSWQPSLPPKQWDQHPVSPPLHPLQHQDPRFLLFPAAPQQQQHPLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4L9</accession>
    <name>A0ABQ5S4L9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="638"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="882"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1089"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1227"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="898"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1323" mass="138072" checksum="0A4FFA35C51DA322" modified="2025-10-08" version="1" fragment="single">FERTINKHARYICYVANIINLEVVPFVSYNFSRMLLQCRPVQWYSADAYICKRVQITNVSIIRCDGLVQLQQRSDIFRRQLVRRPATSSSSTAAIPNSPFDIARTLRDLAKASLVPRDHLARADLDVRIDTLATQFRSTTADGTLANNRDLGSIVWSLAKLSTPCCCGHAIALLTALTSASSSSPASLQSASNQTLCKALYGAALLSRNSSTRTAIARISRGNSSSETPILPTHWSALLPPARALAAACVPRLASFNMLDTSNAWWAIATLASLPPPPSAAFFGASIPVPSRTGLASSKMQKQHKHQRHKVRTPLDGEWEGKEETSGVFPLPEGLLAGLATAAERHMRTPNGCSTQGLANMLWGASRLGYADERMLSAAVALLKPQVRTLASTDSRAFATCLWAFASLHYVGAEGLLSAASRPAAADVGAFDDQSLANLLWAYGTSGVEAPKLFAASADELARRAIRMPIEFLAICLWAFATVNHLDPNMMAVVAHSLTGLSSTPSPAVAMGLAAGKRAKHGRHAMNNAVAQAVPRPAGLVPTADWEAALAERLAACKGRRTQAVGNLAWAYAALGARHDPLVWALAGRAAAMATALNAQELSNVLYSIARLVPYFRETRPDVLEVLLQEVDRRVELGQGQHDQAGTNRRSRRNQLPREQESTTKCLRKQTYSAETVLVEQPATSGLMPHSPVSTTSEGTLSPAQRFNPRDLAMLAWSLAILAPASPTLLRILKAAVAAVDSSSTDPTSTTTDTGDAADAGVTELDELQTQPYNLFGLVQLYGANYAACLAMTTLDAIGGSADNDLQDPKAMVMIKMDVKERYGDRDYSFSKGCGDGGCSDGGGGGGGGRTMLVRAAAAAMLQAQRAAEAIGGDQARGISKCTSNSEGHNTGKAASAARKEGEGGRRGGGEGSMSGQSRVLKEMVRFGPLGACPPLFRACRDAWFLNTASTKPSDLQSAVAALLRNHRWGGGGSDGGIDSSTRGSCGGSGAVASQTRGVALVAEEALTSDRMLRVDLLVQFNGWLVLLEVDGPSHFAVELNGKPIDTANGDSSSFRGAADTMRTLGGTGSGCNNDGAAQGADIGATAYSTSCGKENEDGDPDFNGSDDSGDEEGDRLVTDGLGEGNPARGNSKHSAAAAMALRRQGLLRPLGDTVFRNRFLVASLGLLAPGLQEGASVVHASTGVNDPWVIQSLLVQLSPGIRPAAAAKVDGKAALAANSESVAERHGCAGQGQGQGRDGDAVGPTAGVAGTGGSDGGGTACAACASSGGAIDTNSIGNGNGCGLVRGVAVATLSYAEWNAAGSTPGDKWARCLQPLLAQYLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S557</accession>
    <name>A0ABQ5S557_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008443</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI65028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046461021" description="Pherophorin domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="656"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="71693" checksum="F521C1B7C3951609" modified="2025-10-08" version="1">MFAWAMRSATAIIQLLVVSFCVCFAAALQRPPTPTHPVRESFSADYEAQSSSSAVSIPQPGMPVSRPTISPLLHMGPSTPPFGPLSPSSPSPKPPKPSFAGSPKSPRSPLPAGPSLPFPHPLFLPFLPEMRLPTPPSASTPVPASPPLSPVAPSPPPPPLPSAWNRWPPRPPRPTLPVLSAFPSRPGQPFPPSPRPPRPSPRPPLPRPSPPSPLLPPSPPHPPRPPPPDPPEDLLILEPPLPCAWVTLYGTDFRSRRFLFPPTPDPVASALPDLVYTVEVTGVMPVSAVAVCPSAQPCFRPDLADGNPTEALYLQPSTGSLSGSGAAYYSRSQRLELRGRDFLQGVFVRARTDGSHRNYTIEVCARLPQAAPCSNFFGIMSVMRTSDAFSSEAVLSPNRISPPPWSQRLAQLAAVAADSPAAILTSTCVPPPGISNLRKVVFSWGWDFGGLPPSLASSGFVAVYRRYPLTTMSPGSYAVKFMKPENVSLAVIVRVKDGNPDVAGTLAEVREVVAASEATFLTIPQGSVIDITFLFLIPPLQLLNNIPQMSAAIDMTWRPSPPSPPPAPPLPPSPPPSPSPPSPPSPSPPPPKPPSPPPRPPRPPRPPSPPPTPPPSPPFPPSPPLPSPPNPPPAHTNLQSADQSGSSASLRRLRRSYGVVPTATLLGFLFVFVLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5J0</accession>
    <name>A0ABQ5S5J0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008609</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038791">
        <property type="entry name" value="Cfap97/Hemingway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23035:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23035">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 97-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="410"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="160"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="419"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="530" mass="53595" checksum="D15844C45D1A456F" modified="2025-10-08" version="1">MSSDLDDELGGEEEEVIAAPDDAYDDDDFDGVRGDAAADDEPTRAQTSYAADDEYKDDLEPEFEPEPSRKAATVLDEEPSYQDDFEQSMAPGLSKMPDEEEDRDATSAAMDVSADNSVVGGAARAPGSAVLAKSTSGASRSSSRPGSATAAAPPKPSPAAKPSAAAAALKPTAAPKPAAAASTKTAAVAPKPAGAPAAKPLPVPLKPAVSKPAAPAPKAAVPAAPKPAVPRATAPAPKPAAPKPPAPVVAAKPSVPARNMVPAAPAPVQPAATGVGLSGQKVKLTFTGPPNTQRAAPTVKPDYSHSNYEVNKINSDNVKLCNRLVEISRAPPNPAYNNALSVGSAAVTGKLAPANVAAATVNRHRKEDEIAQENLALYKRLQAVKPSSSVNREVLNKEFVKTQAYGENARKVKVPPAHNTSTSGATAGANATKLASSGATRQPPPGSAEEVYSRPSGSGSAGGGSRGPPDGRISNGEAAAAADLSTVMEGPEGEVSDGVYAAAVAAAPNLEPEYSMDPEFDQAPSQSFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S624</accession>
    <name>A0ABQ5S624_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008719</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050966">
        <property type="entry name" value="Glutamyl_endopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15462">
        <property type="entry name" value="SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15462:SF8">
        <property type="entry name" value="SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047481884" description="Peptidase S1 domain-containing protein" evidence="2">
        <location>
            <begin position="31"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="67493" checksum="AFA72ED208B65E35" modified="2025-10-08" version="1">MLAGDMMKLPGFWFLCTCCWLAGILVSIQASTSGGCSSCPLTYAPVVDADTGLPFYNECVAKCQLSPGANLVPAGSRRAAAADRSASASSQPDFLKLVPAPTAEASKDGGEVTSDTILRFTRQGYNYVGRTGLSAMLHDDKVTDKDTVTSGDANNTATAALSRTNEHLRNQDGATNGPTVTLRVIYPQGDLYMKVWNQSELKALVKKHTGPKSDSARLSLASIPAVEAADSDDNIPAPPMQAVSGIPSRQSARRLLRVKALGVDDRGEVPSPSYPYTAVSLLSTGCTGALVGPTGRLVLTAGQCLYKYNNDTKSPVSWYSNVTVAPSFHNRTVAPFGWIPAEGADVQVMWKSEGIWAANIGILRLAYHCNLPGSFSFGVRCGPLTQTLTTAGYPMDKPQFTMWMQSTVVTTDMCSGGEEKVFPFDAADGQVGSPFWNSENSVRFVLSRGSCCSNVGGAVAITPTYYFWMLDYTETNIVTTPVYNLVGGANRDQYIRCDAKSLRCLGTKTSSLSTSFYAYPVYSTSKYALRIPNTSLCLSLLQTNNISTPSVGLLKCNIDTFTFPPNSLWNFVTLDNIAVALTDSGFNLLWNGDTRQLTAVQNCALLSSPSQCKWYLEKIVSAAKKTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6M4</accession>
    <name>A0ABQ5S6M4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="256" mass="28677" checksum="00763E87B2D26ED7" modified="2025-10-08" version="1">MTDLTSTGPKESYNKTLRAAWQFTNKKNDALQEQVVGKLGIVAPCPVHDSGADMATSDNARSRVISSKTYAMGRTGLTLPWLEACQRFSSAGAVDGAKYLTSCLSKQLKDIWGLSQSQLELCQVTVRRIYYLYYAPYETVVYVHSGRLAGTLFGNRTEAAVCDVSVRSDENGVWYGRVQLLLSMTHTQETHDLVYVRWFEAPEDWNRELHLRPLKWHRKPGAPHASFGYIPLESSLSVFALFGPRGRLWGNVGPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7E0</accession>
    <name>A0ABQ5S7E0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009465</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="161" mass="17759" checksum="AA8F2A3A347AE91C" modified="2025-10-08" version="1">MRGAGLSTSDPWLMASVLPEDDKRESSTFITHRSGGNTSSSGQVQGSRQKSNTTCHYCGKPGHLVRVCKTRLAHQQQGNCGHGGGFGLPSVQKPNKKMYAVGIKLRLDAWYLDLASEWHITYDMSELEDVRAVQREKMFTVVGYDGSRISQCTLVTVEQHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7J2</accession>
    <name>A0ABQ5S7J2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008630</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02663">
        <property type="entry name" value="Peptidase_C19G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF733">
        <property type="entry name" value="RE52890P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="7">
        <location>
            <begin position="58"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="421" mass="46669" checksum="A05BA3E211F5F753" modified="2025-10-08" version="1" fragment="single">LLLFAEHPSWFSLDTLLIVHTGKKSGQTVAPGAGAVMGANASKLEKALAEAPEDERYYGLENFGNTCYCNSVLQALYFCKPFRERLLKYAAALPPTTEENLLNCLAELFNQINSSKKKTGVVSPKKFVNRLRRDSELFRGHMHQDAHEFLNYLLNQSCELLEQEAKANSQQLQQQQQGGAGKGAAAGGGAGAGAGPAAGVAAVSPTQAQGCVPQPPITTWVHEIFQGKLVNETRCLHCETVTCREEVFMDLSLEIDQNTSLTSCLRNFSSMEMLDRDDKFFCDHCCCLQEAKKRMLLRSVPPCLILHLKRFKYLEAQSRLRKLMYRVVFPMELKLANTTGDDPAAGDTLYNLTAVVVHVGSGPHHGHYVSLIKSGPQWLFFDDENVELITESQVQSTFGSTSEYGQNNMDHGYILFYERAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7Z8</accession>
    <name>A0ABQ5S7Z8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="130" mass="14877" checksum="636707C9032084DE" modified="2025-10-08" version="1">MQQQQQQQQQQQHLSGLRTTRPLRGYRNCARTTIGMHHVITRHPPLNLSPFSECRAFGSGGGKGPEGFLKKLVEDAHRHAHRIIDCLTPKHEYDFADVTLIFISTMAFMWLAAGLYRVYAFSYYLTVSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8I8</accession>
    <name>A0ABQ5S8I8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Adenylosuccinate lyase</fullName>
            <shortName evidence="9">ASL</shortName>
            <ecNumber evidence="4 9">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_009929</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="4 9"/>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01598">
        <property type="entry name" value="PurB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047136">
        <property type="entry name" value="PurB_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013539">
        <property type="entry name" value="PurB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006764">
        <property type="entry name" value="PRK09285.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43411">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43411:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08328">
        <property type="entry name" value="ASL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="11">
        <location>
            <begin position="79"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Adenylosuccinate lyase PurB C-terminal" evidence="12">
        <location>
            <begin position="398"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI66166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="528" mass="58568" checksum="40A9E7229B0003AA" modified="2025-10-08" version="1">MNTTLRSSSKTGLMGRETAGRRQPYLAVSRICPCRPLATLAAPPPASIPSHNAPKAASTPAQSPKLELSELTAVSPLDGRYGRLSGPLRTIFSEYGLIRYRVLVECRWLQQLSRIPEVTEVPPFSPEANKLLDRLCTLEGCSTEVAARVKQTEKTTNHDVKAVEYVLKEHFRSNPELAAVLEFTHFACTSEDINNLSHALMLKDAVHNHVLPTMDKVVSELGRLAKDFADVPMLSRTHGQTASPTTMGKEMAVFAYRLQRQRDQLAAVPYLGKMAGAVGNYNAHMSAYPEVDWQAVAQEFVPSLGLEFNPYVTQIEPHDYIAELFSAIIRFNNILIDFDRDVWGYISLGYFRQRTIAGEVGSSTMPHKVNPIDFENSEGNLGLANALMDHLINKLPISRWQRDLTDSTVLRNLGVGIGHALLAYASTLKGISKLQIDTARLAADLDNSWEVLAEPIQTVMRRYGVPEPYEKLKAFTRGQRVTQESMMTFVDGIEGLPELAKDQLKKLTPANYIGNAAEQAANLHHYLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8J9</accession>
    <name>A0ABQ5S8J9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_009373</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="202"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="144"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="324" mass="34433" checksum="BD1F720B3C71191E" modified="2025-10-08" version="1">MSYEPPPYGGGYQPPANHGPPPSDPYGHPPPSYSSYAPPPYSYPPPYSAPPPSYQSGPSGSDEVRTIFVTGFPADVKERELNNLLRFLPGYEASQMNYSKGSAQGFALFGTGAMARAACDQLQHVRFDENSSLRCEMARKNMYIRDMEPNKRPRPNAPPTGSYGAPGGGAPAYTARTQPSFGSGGGRSGGDHGSRDDNPPCNTLFVGNLADTVDENELRNMFSNAPGFRQLKLMRGPKATLGFVEFDDVPTAMAAHVAQQGAILASSDRGPIRVQYSKNPFGRKRDVDGRMIETPNTREGIVPATVGSAPYSGGAAGGDYGASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8R6</accession>
    <name>A0ABQ5S8R6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_010113</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF254">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN SHAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="472"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="14">
        <location>
            <begin position="281"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="75"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="669"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="789"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="676"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="790"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="803"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI66333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1063" mass="115399" checksum="7C0C7FB8F0D03AA5" modified="2025-10-08" version="1">MISSVATAAAAEQIIQERRRRQLALAEEDNEAQQEPSPSSSEESILCEEAPTIHQTVDTFDSKIMYTEFTPDALGSLPNGLVTQQQETDRAPVTPASGQSKAKLVTSPTDAEVPGQVDLQGPAAMTVPPVAAPTNHVARLVDDVLHLTNERFGASLRVQLKLADEVLQGVAEAADESERGDSITLTQATHLISQDNLSATEVTARGSPRFAAEVEDVYKYDLMENNPNWKSFLTSNTGVKAQIWESKRQAATNCWGSLLYNRRRGSIYMTLSYPDFSPLAYWFGVFMICIILLNTITFCIESMPRYENTKVYSSLMIVDYVCMGIFTVEYMLRLLCCPNLRRFFWSFSNFIDLVAILPFYVELCVKGTSTESEAFQTRIVRLLRVFRVLRIVKSMPRLRHLTLVSDTLRDSTEILIMMACLLLVVLVVFGTIMYFVEPSTFDSIPQAMYYAQTTLTTTGYGDLYPVKPWGRFVAGAAMLLCMVVVSLPIAVIGGNFSEKWSDYKEFRAAVDRSSRVYPTARELRGELESLMPFSYRYEKVLDDVMRRLQECEVASEKQLQIVREEIAAARTALEHGDGGKVGVMSTTAAENRLFHVAKNMMRRVRHGSSGALVRHGSYTAVPPTTAAPSMRGSAADLIEMADSTVCANSLSQGLQQNIDLRGSAALPERDEAQQEAEVGGAAAAGTGSAGLRRGRKFKAIVTSVIRNQVRGQTAGGITMVSEPGLGDNTVAVASLGPPKIACGPTHLATSSGPATAGALSAGALDASAHSSRNSGGPSLPRESARFCKSPLQQNESSCAPQLHVPPPPTPPAPPPPPPPPLQQPSVTIVSHETLGSSSTGAGAAVSPRTSLSAFKRHLSSSITLLFPRKSEAVNAEAVAGAGLDESVPTATPQLQVEWSVLEDAGKRLELAARTCSRAQNLRIRQEAFAAQARILRTDEMPAKFEKLDEQYRLLRCWQDELGPMAERMWCLRDALDELAWGLQEGMSEEERKYSPASREARGMADAVLGAVRRKPGADADATNRGGSVRAAASAATTILRPEGVVDVSTRTGSTRASVTSLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8U8</accession>
    <name>A0ABQ5S8U8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="19903" checksum="44DC0571A01CD8EC" modified="2025-10-08" version="1">MKVRWTSTGIKRKVPVEDKSTDGDDNNDSYNHRRKKVKSASEDVRCTPARRQRGSSGSKKKQVFTMGKDRLDEPLDLHNYSTWKLRFQLLCAELGYSRALLQAPIAEADVIISDKVKSVMAKNVRNHHLQTIVRAANAKAAWDALAATFSSTCNTRKVALRQELSDFKMDFFTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8V0</accession>
    <name>A0ABQ5S8V0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="216"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="375" mass="38969" checksum="040F0FE72C0D7760" modified="2025-10-08" version="1">MEALVQLSEVQRAFVQFCGVLQELILNHQVAHSVVQSLHNDLPALQPERKGPPRNATPSSFGTGAAVGDATSWRPSSSSFSTLEHLQHLAPRLTPTAAASGVGIGSLGADPLYHIASECQDNYVILQNAAHRSRVLLSRLQQLHQSALAALEQQSLMPADGRTSDSCYGGAPDPDPNPDPDWYSEVERSYTGLPTATDGTVFSGFDVDAVTYTQPAVGTTADGGTAATTNAVSTPGPGPLGMPQPPSNTSGRGLTNLAAAAGMTTPSDAVQRLPESDLVIVMGELCRGASLELELLVCIASSIELQTPADDLAVYDIMLKLQPYTDERIVAAALVQRDLLLPVRSSEGRGEGPSSGQHGKGGGGSSRSRGGTAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9R3</accession>
    <name>A0ABQ5S9R3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia- and flagella-associated protein 157</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010531</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="416"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="114"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="423"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="481"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="61360" checksum="A184E28900A3A0DB" modified="2025-10-08" version="1">MEPTRMNSLRPKDLPTSGEALQTFASPSNQDEPLPLLEIHEDLLVRAAVDSATEALQRELTATLLKLEVQSEATIRAHEIIKELEQEIANFHSEEMTRVAEHELLMQAVAESARERSQRDLAVMQEDNEQLQAALSTSFNEVQQARNENKLLAAKMATTQQLLAVAETEVHELRQQLDEKLKIADDEAQEMHQRYCSEVLALRAELQLEKETVTRLQADNMEAVAQLQAAKEAVTRLQMEMAEAVTQLQVEEKTVAQKVVVDGSVLVESRIEEDGEDVVLLRARCGALRRELGSTRAQLASVTKVWEAAAQGRLAEIMAEAHSAGDAGEVKNDEDFWARNSEVHALHTLVTSLRATRLYAGAVGAGSTYGDNRADGAGSVGMASSAQLATSHRRTRLLQLRETLGQMGELLSGAEATVKQRRQQSSLQQLQQPMRLPSPPSPPQPQPQLQGQGQKGSQASVWGRVFSLGGSSDRSRGPHKSGSSASSSSRSCTDSCATLNSCGANSLHDYSSKCGHSMGLRSSTESLQQVTTVGTVADELADLYDLYQIPVQPNYEFNTWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9S0</accession>
    <name>A0ABQ5S9S0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="64"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="218"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="741"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="862"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="939"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1106"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1491"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1581"/>
            <end position="1625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="759"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="879"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="940"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="951"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1074"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1126"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1168"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1303"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1326"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1402"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1494"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1593"/>
            <end position="1602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1763" mass="174386" checksum="BC088562077D0C6D" modified="2025-10-08" version="1">MTNEGKLAAETALISDAVSLITKDRAARRNATRDEVAGWILSSPESRTSEQVSSIVSLLSLFPKLRTAGEACLASLARQVAAQQLSRGDVLFRSGDSADCVYLLVDGTVVMIDQPPYHHHHHHSHGGGGQKRRSSGGMGTGGDAREGRPEDVLQRFNAWELVGDEPVLAGIRWPCRAQVSSSRATVLVVPRHLFHETIALNVRASFNRKIDFLARLPVFGNMPRRVVERLTPFFYEMELGPKELLCRQGDPADCMWAVVNGRCYVVIDPNWSPAANRPPETDPKKTTQVAAVQSGQLFGEMCVFGNVRHRTATLRCATDVLFYRLKRLHFLRVCPPAQLEELRAIAEAKMMLLVQAQREEQGAAARINRVLRGHSLRALHPKHRAQLLRGSVDRCPASPSGRLQPLSPTAKTSLHSPAGGGATSAAELGAIHSGGGSGTASGEGDVWSDVETPKPRDQKSSSVLDLDADVSHSPWATISVAAAEFVRPSQQRAMTPQMPAAVAAGTGLGARLETVLSHFSHVELVDGLGYSDDEDHGNSGGGGGGARGAAGGGGGGGGRGVTSASASSHRLLPSAPTVPAPTTVAALPFGWGYTFLRPTESGSGNGVAGGRPSEAGGGAGGSGGGLEGRLISSKAQVAVQVSGAKSSVAALPLAATLSGTPRVSESAVTLVAAGASSLLSESKGSAPSRFLSSAAPMHYQTSTGAGDGNSNCTGGAKAVGVGSGAAGGAASAPLSVLIASSDASGDGQSPLDGKGTDNAAGAGSDSDSGGSGSDTNEAQYSYRGDVDAADADASDATQQLLKHVPLQLLPLVDCSWNFGPCSVLNVLYARSSHSGAVAAAGGGGGFGGSGIASSRMRPSLRGGPIANANGGWDGRRTAGGDGGASPDAGAGGGDGMGAYTSPEPGLVSVPHLVIGRCSTPDTLGPWSARERVTEAAAAAPPLPLPAPIGPPRSVSPGAGGSSSDPSKAKSGCAFGGGFGNVAAAVAAAAAGGGVRASSSTAGSGLAPLPPAISTAGVRARAVPEQQQQQPYKQYGGTRRRCGPPGPTLGGPHSSLFRIMRAPSELPGSRRESHAATTSAPAPATSAATAAATSAATAAATAAVAIGESSRGGGTPSSRRPRFSNHTSSPDASSPPSQSASPGPGSPRRTSSEGLGAAGAPMGRLGKVPSGTAAGAGAAPIDAAAASQSASTSSAAAATAAEAPPVAPASQRCGDSRGSSNSVSAVDEHPGGGGGLSRRVRPPPPVQVAPPYSQNSPVVVKRTQQRHLDLTTTSGVSNGFDLVSPSADGGVAGPREAAAPQEDQPLSYSDTQSCAASTLRATAPGSVPSPLPGRPPAPPPGPFSAPPTGSGSFKKPFARHGSAVSTANAVGSRQKDEVTAPPAEAEDSVPSCTSYGSLTGDGVESGDGAGGGGGADKGKGGTIIIDDDDDDGAPSSTFTTTDGVAAALVAVTDAAAGGDAATPDGAIEGGNEGGSGFPASAISIAEPLRNEEYGTSGTVSSSKQISSTRSTAPESLRQHTYGDAWNAPPPPPNTWAVVPLTAANAADTSPHASIGPLSTPFLQYMRAAAVAAAVGVPASPSAHGISGGGSGSGNNTHLPQLRTSGMGGGGGPLGPAKRRSTHHSSEATVAQSLAAHRNVLAAAAAALSTLSPAVGGAMAYGAGSGPSPTANALASLLTKGGCRTSLPANVRQPNVIVKGGFVYRAATCKQRLSELVNGVSGAGGAGSGMGGHGASSGGGLGAAAAAAAAAAAAGGGGGFGGGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA55</accession>
    <name>A0ABQ5SA55_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010588</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="168" mass="18105" checksum="494D43ACAE067E89" modified="2025-10-08" version="1" fragment="single">AAAAVTGSDLNILAQSEVGLARGRTYWSRRSSAEKVGYAGKSPIPQAPPRHRKLLRTRTGSCTESSSPSIQPLLISLAGAPRGPGGAVLQSPSTYHDAEYDQVEAPSPAGSYMRPFFTKSFRYARDARPGLAWVSPFRDRTDPHCHTETFHSVGSEAVNNSFSLIGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB22</accession>
    <name>A0ABQ5SB22_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1088"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1280"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1314"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1387"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1486"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1544"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="578"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="611"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="625"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="700"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="764"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="797"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1100"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1156"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1187"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1280"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1292"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1323"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1344"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1409"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1447"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1486"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1514"/>
            <end position="1523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1544"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1555"/>
            <end position="1571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1650" mass="161968" checksum="2B8C2C5321002E23" modified="2025-10-08" version="1">MTAGDAASLSVEGLAAPVTMNDRRVIEALPTISALGGIAAVAEAAVAAAAAEAAKADLECIESDGGNDGDDDGQHDGGDSSGGTSGEINLNADEIMSGVFAPPPLETLPILLGPGPFPLGPTTIHDDELDNGAGRITPTTGGGELLVIDAPTPAAAVLPPQRVLTPRHGDLLDGEPDSEPPSAYQSPTMSPGMMTEKTHGYGMGVSGGAASSPSAAAKVAIPAAAAEKMMVLWERRAAAAAAAASEGADGEGNGGAGDVGAAGCSSCGNNNLTLLRKVQSLRSPGRRGLGAAGLHSIQSRWRRVSNAFTGIVRAHKLVAADRSEFVCDNNSALMQAQTGELDVALEVSNARDLDKINYAHQGNRDMYSGDMIHKRRGLRTHPQVMEAIRAWWRWLPKSATRVVVVMGCGGDEEDEVFAATLDEPIYSAMVVAVTMVLLPRFGLPVDERYDPKEDWLDDNKGLPYMDFPNFFDALFELADMWSEGVGGPEYAELLRFLLHGVQDMERRQGKFGRLLKRFGHKPAPQSLHGTAAAVAATAARGAGTPAKLQAKSKIGAGGGGSGGGGGGALKSSPRASIAGYGSSNSEASGSNSNGGTNDGSGGSAGGSHQYGNDRRRHDRLGDSPRSPPPSSPAPPGGKAGRLSETPRGKPDPRVSPPPPQTPTLSTPSQVSALLSTRSSSDDHLLSAWQSQRRLPPMGSGSSGGTNGGGGGSGADGTEEPRAPPPRSSRSPAQMHDYEHVDYSNIPSTLGAYLRQPLPKPPPKGGGGGGGGGGGGGGGGGGNKGMDGRRVSDSAAGAGAGGGGGGGSNPAPVAAAPISKQSSDNDKAGDLASGGVGSVSGFPPSPSLQSPAAIAANGGGSSIGGNLSGQHSSHTVFGGNWQLGEGFLAQAGVDVATAAVGSANGGGPGGGTWTYGNQTVSAPNGFGGSGSPLPRRGPGVGGVGGANLRNAMSMDLPDDMPYGQLGSPGSLYAEYMDAVMSRRALRQQGGIGGSSGGAAAGSPSAAVAGLAVLGAPPPLGPRSLQHAVLTVSAVGSSGNTQRESLSPDKLFATNDPWVTTAGGGASAPGSGKAVAPIKRPSRLALLNVTDDSVGGSEPPFSVQSPAASTELSLGLSPQAREAPPPSQLPSPAMTALSEEGGRGEEGGWEGEGEQEWQRQQQRQQQRQRTGDVGQERSMLPSITTSSSDRADRTDRGAQGRRDSPSSPGLPPPPSSPSSWFSSPSPMPGPPRNTRHAFLCAIAPSTSPSTPSAAPGAVMATVPLRATPSAGICLVEISGVGASGRGSGDGGGAATATDGAARSRGMGPAPILISVNSSPALTTHGGAGSGGGGGAGVLPSNVGGSRSPGGGGSSGGDRPQALAALAAAAAAHLPTINHHSSAMLHDMGVGAGAHMHGSPPSRFHGQHQAPAGGGGGGGGGSGGGGNVAAVHGNPSAPPPPARVSWGSSPGTGGIGGSGNGGGNGAAGGGVLLPHLSLDSRVGISSNGGVGNRSVGGAGVGVGGANGGGSGSRGFEMPPPPPPPPQARRDVVQASRAQVLSMAQALLAQQQAGSSSGAPGGGGGGGGGGSGSSGGSSRLESLRPLDGGLKRAQCGGSFLTYLQQGLHSSGGSAAAATSAAAGSEGGGGITSGRGHLASAPRAAGPVALASLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBW3</accession>
    <name>A0ABQ5SBW3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011344</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF123">
        <property type="entry name" value="OS04G0645500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="280" mass="30746" checksum="75CE9339A3626196" modified="2025-10-08" version="1" fragment="single">MSKARDVFRSNESWERWNTHQTTMVEQEVFGSVLRFVQDPSSEHLGTTVWDASVVLAKWFEKNIRKGDFSRAKVRGKRAIELGAGMGLAGMAFAMVGADVVLTDTADVLGLLRINYENNLSPAAVRLARAHQHGTWADSAGSVAVAELDWTKPEQVRVSPLNPPYDFVLAADCIYHETLTEHFHRTVMDVTNEKSTVVVCNELRSHSVQGRFMELFTATHTIKSVAHSKMDDTYQHPNIFIYIMKKKKKLSSNGKEGGGGGGGGGDGSEEEEDCPGCAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCA6</accession>
    <name>A0ABQ5SCA6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="565"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="581"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="606"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="76023" checksum="8662E84195732E7C" modified="2025-10-08" version="1">MQNSVDVFITEAELAEGRRAKRTTCMKVDPEALRPVNCVSRSGISSGTLFTRSDPQRTGRYLTDNEMATMFNKFVGPNSLNNAAVRLEQEERNASTQPSPSASTSTCSKASRTTCCDSGDRTPPPPAASLAVAMAAAGCDVPSQVHPPAMGEARERGANAAMMKYVSPKIAKKYGVQQSIKPAGPRAAAAAAARAAGGRGVAGRGVVGASTADRLQAADSISSYSGGDNLPPRPQSASFKTISRDQAQRVGSHFVFGGSTEFPAPGKYNPKYGATDKAQHAPLFSKPVQVVMGTDGGAPPSGVARSNRPGSAPATAGGRGSDSSRPASAPTHRMADDNIDPSAPHDAQAAAVHLQPGAVAAPQRRGLSRSLSSQGPHPHLTAEGRAQLLHRHEELFYAAHRVHKQRPTGPPGSQPFKATERKSLYDTVPVSQLFGEYYKEGYDSLTRRSARPSSAFQTQSQRQYSNGPLGETGPTVGPGTYGKELPGTKTGRHVQAGHPGLTRTPDWSRTTPRPTSAPPGRPPPSSALCHGYYGPDGPIISLFPDEPEYNEINAVRILQERTSQRVPGLPPRAPSQNQPKQQQQGAAAGAGGSASQSTRSPSQLGTAGGGGGLSAAGPGTGSKEPILVYVDPTRYGELTSSGLAKRVPGGTFGTATREASCHAMRVIAKPNVAPPAAVMVKPANNLSYDYNIETEGHRGRPPAWALPPNRNALSQQWMAAAATAYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCC8</accession>
    <name>A0ABQ5SCC8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosomal protein L2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_011487</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_uL2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="7">
        <location>
            <begin position="109"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="6">
        <location>
            <begin position="196"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="294"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="41854" checksum="3761866017CD4189" modified="2025-10-08" version="1">MLRRLAQSCANLLTKQTYSVLEFAQPSTSKSLFGSGHEALQSGGGSLLQLIRSKYTYKFKKAAPLPGLIYWKPTTPGLRHKITIDYEALGVYTGPPTAALSRHVEKTGGRNHTGRITTRHRGGGPPKVLREVDFDRRKLDGLPGVVQRVELDPNRSGFLVLVKYSPEGELPFLRYHLAPAELKPGEVMMSGPGAPVKPGNELPLRDIPIGVPIHSLELKPGGGARVARAASTSAVIQSKQEHYAVVKLPSTEIRLIPLDCRATVGLVSNHLQNLVNLGKAGERRKRGWRPSVRGIAMNPVDHPHGGRTNGGRPSCTPWGVYTKGTRTRRRNKGSNGFILVRKGGQLIEKFVQARKWRARLKLEKLKESGGGGARKAAASAGMQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCX1</accession>
    <name>A0ABQ5SCX1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011199</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="491"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="216"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="737"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="739"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="767"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1263" mass="141379" checksum="3B9BC25133453419" modified="2025-10-08" version="1" fragment="single">EDVLAAIKPNDDKLARGTIKKYILPEDLLQFAGNTTARDLWVALAKKHIPNVRNFRMQIQQQLKDFEMLSGESIDDYFRRFYKIGYKASLISMKIEPEDALLHLIRGLSSDYDGVKSVLRHMESIESVDQLQDMLKTEEGELRLQRNKAREVSLMSLSRTHDGARVGPSQGAGAGNADGGRSRKACLYCNKEGHFIAECRKKANDEQRGIWRPALKGLRQKPRNPPALAAPPPAPPPVAPVAQPPAAAQAAAPPRPAQGQRRQLIARVYSSQQDDWERELWLFDTGAEVHVTGDKDKLRNYQHCGSDCKVWLKTANGSVVRPEGHGRVMFRSSCFETDVEVDVYHAPGIMGHILSLKALEGSFGPNVVPQFSGVLPHVEIGGAPAIELEKIDGLFYAEDSSYDSWMGRYGSDRCLSGKKVREELRKVKLAITKQDSNLGVLWHKRYGHLGFDALAKVASSDMVTGFGASVDDVVTAKGQFCKPCAEGKQTRQPHPSTGNVTSKRLDLVHMDLCGPLPVKSWNNAKYWVTFYDDCTKFSEVRCITDKTVVPETVKEVIKLWEWQTGDKVRRIHTDRGLEYVNEELASTLRDMGIFHEKTAPYTPQQNGAAERLNRTLEECVRTMLADSGLPQEMWSEAVITANTLRNVSPTKGSNLTPWERFIGEKPDVSKLRVFGCPAYVMIPKQQRHKLDPVSVPGQFVGYAPGMSAWRVWIPKDRRVRISCDVKFDEWGVRGSMEFDASESEEEDVSGSDPEADEGNEIQEESPPEQPPEPAPQPEPELDVPIALRKSQRQNTKYAKSILGVNVDIPATYEEAIASPLVDFWIEAMEEEFASLQEKGTWSLVEKPADKQALGVKWVYSVKLDKHGVVERFKARLVVKGFMQREGVDFEEVFAPVSKHSTLRVFLAMVAADDMELHQLDVKTAFLNGDLEEEIYTVQPPGFESGGPGLVCRLHKALYGLRQASRAWNKTLHEKLIQMGFEVSNADPSLYIRRDLDGQICTYLLVYVDDVLIASHDEQTVLEVKEEIKQLYECRDMGEAEVFLGLVIKRDRETRTLTVSQEHMIHELIERYGMVNADPRSIPMSPGTILMRAADGEVLDRSRYPYIELVGSLMYITNCTRPDIAQAVGVLTRHMSAPTEQHWRAAKAVVRFLLATPQCGIVFGGERVEEGLIGYTDSDYAGCIESRKSTGGYVFTLHGGSVSWSSRLQRTVTSSTMEAEYVAASEATKEALWLRLLLSELGYSLRPTTINCDSQSAIKITRTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD86</accession>
    <name>A0ABQ5SD86_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF150">
        <property type="entry name" value="OXIDOREDUCTASE TDA3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="47"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="459" mass="47497" checksum="923BA13C162CA526" modified="2025-10-08" version="1">MIVRAAFGTTRIQTDGPRLKRPPADRRYASKAMASGVQGGNERTVPRIVICGGGIIGAATAHYLAEKGFAEAITVVERHEPACAASGKAGGFLSLGWNDDSPVGPLARLSYRLHEQLATQLGAERVGYRAVYTLQVVGVEKPPAGGSSTRLRGSTKLPDWVDGHVVGMSEMGDPTDTAQVHPHKLTTALLESAVKRGSRLLQGVVEGLQLEETAGGDDTGVEAGKGACRVTGVVVDGVVIPADVAVIAMGPWSGVAKEWLRTGTGSVRGGVGAIGKSAPKAGAECSATRSLLPGLTISGQKYHSVVLRPHKSVTNHMLFTGFKYASGRVVEPEVYPRPDGTVYVCGEPQTLPVPPSAAEVQVEEQLGENIRTVAGSLASCLAQAPVETLQACYLPCAPDGLPVIGPVPQCQGAFLATGHTCWGILNAPATGLVLAEMIAEGRASSCDVTAFSPSRFAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDJ0</accession>
    <name>A0ABQ5SDJ0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_012210</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RETICULATA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021825">
        <property type="entry name" value="RETICULATA-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31620">
        <property type="entry name" value="PROTEIN RETICULATA-RELATED 2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31620:SF15">
        <property type="entry name" value="PROTEIN RETICULATA-RELATED 2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11891">
        <property type="entry name" value="RETICULATA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI67998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="319" mass="33270" checksum="09B937AB065CCFE8" modified="2025-10-08" version="1">MESLTKPIQLRAPVARPSVHRFPVPTQTRRTLRPSQHVAALAGAGGGDLGGKGGSGGGSGGGGGGDGKGDDSSSNSPGGEPQKGGLFRGWEERVAYDPEFPIKVLLEQVIGVSACVIGDMSARPNWGLNELDFIFSTLVVGSIMNFSIMYLLAPTAGAAASAAATPLIVRLVGDYYLKAWCAPGGNCFEAGSFTAAQRLTNLVYKGAVFAVIGFFAGVVGTSLSNGLLALRKKMDPDFKLQNEPPNVVYNAFTWAVHMGVSSNLRYQVLGGLDPMLVKVMPVTVFRIYQAFIRGANNVVGGMSFATLARLLGSQKAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDW1</accession>
    <name>A0ABQ5SDW1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012456</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF169">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SBP-type" evidence="5">
        <location>
            <begin position="63"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="166"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="401"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="490"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="546"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="981"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1206"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1302"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1769"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1996"/>
            <end position="2019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2112"/>
            <end position="2132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2266"/>
            <end position="2287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2344"/>
            <end position="2385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="214"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="490"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1215"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1305"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1317"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1769"/>
            <end position="1783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1784"/>
            <end position="1803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1804"/>
            <end position="1814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2351"/>
            <end position="2361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2375"/>
            <end position="2385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2669" mass="281046" checksum="2BDAA70B34D1C1C3" modified="2025-10-08" version="1">MDNNRQKQMLGTSEQMGADQPSDEHRASGFQGPQQRHQQSTQDGEVRPQGIPQVSPVITDVVGNRCQADGCMEDLSGLRRYFRRYHVCETHIRSQVVLIGGRQVRFCDQCSTFHPLAFFDGQRRTCRDKLEQNRLKRRIRKAQNVGQQGPAAAAALAAITAGVVPGGGGAQQSDDADSDDQPVNGGMLIDGDDAPALRAQGGRNVARKRQSGISAPAARRQQPAPRQSAGPDVTKALKAQHLTPGHQTRAESQSPESADEEGGRRTPGSGCGPRDDTAWAAMEVTTETGVARISVSNPRVRHGEAGFASDWPRVNNHDLRDQEHLQAAHGTVRNSGMYSPRSQTEHLGPQGSHALRSQPFKGQHVPLPSYSQQQQQSLATGNWDIAAGGVIALERLPQPATQGTWRQHGGSMPNLPQEPSESPTLWPPAGKNRSTWHSVPAIALPTSGTPSDSHTVTERALRDGSVRLTTGPPVSTAGYEVQPRVPQATNGAGFQLSNSVNGSESGFGGTVGHPGRMAGRLRSHMDPGVGAPAVTLLAQVMRTELELRRQPPRTSQPGPEPISPATLAMPGAGGNGGTDLLPGTDGLTPAQQAALRLQLEAGLLSSSRNMVGASQPQPQAGANVQKQWMAVPLDAQQQQLHQQEPTSYGLSGPPIWTGNGMAPQGALWAPSQRQHFASAHVVGTVTGAPGTGLFSVGPHSMVGSRRMSAGSSVYTADEEAHAMGLNEQEVSSLAMEAVAVAAGAHGGTQQQPIEEAPSLLNTQSFKEDIDRDNAKAQIWETQHRQQQHMQMLTDQQHLQKQQQVQIVVSIPNHQQQLQAFQGEQRVTLLDHIQQEESQRNFKLPHQHTQQQQQQQQQTSLLQQHMREQGLKRNANFGGMDTWQTTQQQTVQSRSQTQPGNQNAPANFFGHLYEQQRLAVPNAYPLSAFHMQGPVDDLDGDTDYANLQSQQGNAASVRTVSHGANGKAMTVAQLPPLGGGGALVTAPCPPSPEPRPQHTWPCPSGVWGSTNSNAGAPRPAFEAPPPQQPEDSSAVQQLGNSLAGVAPSDQELILSTLMKFAAHPPRAATTGQERGLIGPPPAHDVQGRGFDPASGTIGGNIYALQQDRHTASQPQPSHSAAAELIPRQDAARYNPKPNNVPDGTQQVALGDNALQPLDSVAIMEALSEGRGSLQLLDSVDFAQMHDLRPNTIPAMEAAECGAAGVQGGPPTPPPHGGDGFAGYHGGIGRAPVEEDRWLASSSDRQIGGPSWRQMGRNVEHYMNGGEWISLPAGEGLRAWERQQQPQQQQDRSSPYSALMASDRCMQQQQNPQQQQPPSYRTQSWSQQQPEDLGEGCRPHAIYRQPCNTDGVPFTGPGRGAGEQTMSHVALKFSHAHPDSLPPDLVQRMQDMLRGGGAAVLPPTLREGCVEVLVEVLHNCSISELCKKLFHLPEFDSATCASLDHAASAAISASEQLRAWVGAEVFDACERVTMQLLGGPVLDCTAGALPKLMLWETAMQQAGLGFAADWPNVLRLHSGPPVLVADAVKAQEIHAHGPAELESRPEVVRLNVRIAGGACPLGAAEAVALISEGRAAFSAEDQAAEAICLEAWQQPSAARNPSHCAAHAAPLLPTASDGVVEAQLLRDLSKPELRSDSTFAFASAVVSAELRNNIQGSSLPQRKGSPAGTAGVTEPPCFAIVASKEGGTEASENSGWDSRARCLNSIATGPAGAPPKIVADAGELLRSCFLMAPLPSSQPSSPVIVLQRPPTQQHRLHGCTSGLLTRGQVGGITSQREQEQQQDSPRQTEQKEQLIRLRSRSKSDDGTTPSRNSNQIRRAFSAPPRVLAPPPKSKSLSVQLSVRRRAMPACTTGNIALISPAVVPGATRGCADVLSGRVSLCSTATLTTAMTLHEKYSCRSSSTALTNATTLYDRMSLRSDWSCISTLTSFGGVTGNAGGAGGGSCSVGGAALFTVASRTCEPLVLSTIFGIPGDFEQTLRSGTSIGIDSISGNSPVAPGAGLSMAGGSPNDNGSETCGKPMPSQPAGLLAEAADDTVVAHHAESRRNLLQTCEHPSFKVLLPESASDDDGSACELTVPTPRLARASVFPSPMVAAAAAAVAAADQVAAAPAQLCDGSSDQRGHEQSASTGHRPADLKSGLQLLRVRLPVLPRAGLVLLEATGGHAVASCWPLLVVETAAQQKELVALWRQLIDKGQEDAWSGLVADLGLVLGILPRRAPACELDLPQGAGLVPREAGSTDAQQSAVPVLADLKPEAASVDARPFPVSDAELSTAASGGPSAYGEGDADNRHDTAIVWQLRRTSAVAANWTHGNQDRPYCDSLRSVESAYQESWGTFATHQSAVSEQRIYPSPSHGATLASTSSGGSGSGGKDTRCGNSSDSNSSDSDCSTLFASDPELCDIAALVVGCSGVVDDADRLSNAPACAASTSPAVNPDGTSAFGHRVTTDSGAHNEETYEFGAEGNPLPSVGCVCVQQRDEPSLASDLGSVCDAEAEAEAEAKAEDDPSVVAVTKYLAGLRTVDDACEDTVLARRDSNDGGLDRETGIDGCDDKGVPTTAAASTGALVGKMAFADGNEVHGKPGFQTAGTAGTLKSPVLPPAAAVGLSKEARAALQAAVSRLQRRMDRRRTPALWELLARVEANDEMKLGTGSGIILAAAAAPAYPTKHTSSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEF1</accession>
    <name>A0ABQ5SEF1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052145">
        <property type="entry name" value="Mediator/Homeobox_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330">
        <property type="entry name" value="HOMEOBOX PROTEIN BARH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24330:SF19">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="265"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="376"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="815" mass="86373" checksum="2FD1AC4B44CD7990" modified="2025-10-08" version="1">MEAQADKGSLNLSSLIQGADHLPAKIELVLHGTSCRVLGCCTASATNIMVMCNCAACVTRSAVDMFRHGGRIMSLDEWCQHTTLGGASFERISVRQPTANYLVPLPAWLRAAALHIGGDTLRRKQLTVFWWNAAPDGPAPTVPPPPSQRLSEPKRTSGAWRAATVVGFNPHTGLFTLKYHSDPKKQVSQIFLPLALIHFARMAPPAGTSPRNVAEGCTPLPALLLPSPDAVATSTPGKLICPPAAVPTHPVLLLELQMQQQQHQQMLQALHQHQHHQPQPQLQERQQQRLPQVPHVNSLVSVQQHQQPNQPQLQLRQQLQQYYLQQQQQQQQQQQQQQQQQQQQQQQQQQLEQPMLLEASDGGTWVQEWLRKRKAAGASGADSGDSSPVRPGSRAMSPVTGFLMGRSNSASPVNLHVLDVPAGGCGMTGPGGMIAAGLSPAPPQLPQLHSRPRRPLLDGQVASAISSSNSSCGGNGGGMQRGGYQRLISSFGSTADLNSVTSQPSQLPNNCAEVQPLEATLVGMEAVVLGGDSPVPSAKSMPMVRSAVGNVVTAAGAGAAVPQPLSGHDASAGFWSISGSAGSCWDPLLALKGHQQRQPQLQQSTARVGVNSGSNCLGMRCAGGITPMSASSVLNADFEGMFDALDSAWFRHSEDVGAQIVPAQRADSGPLPHAIFGVFPSHPSAQFPQQPQPQLPQLLPPHSSRPWWLADEALPPGAVVSLPKRARMMSSGNNVSSCGNASFIPNNNIAACTNTFSGGGGGNGGNGGNCVSSSSSGMHPHDCDCDWTSLLLEARLFDSPTPAPASLPGDMNTMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEJ0</accession>
    <name>A0ABQ5SEJ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_012151</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF285">
        <property type="entry name" value="V-MOS MOLONEY MURINE SARCOMA VIRAL ONCO HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="412" mass="43078" checksum="7125589CFD8126A2" modified="2025-10-08" version="1" fragment="single">PEHIAKHSDAFLVEADILANLNHPNIVRMLGVVTSPPAGDNTGDCNGGAATGVSDPVVGIVTEFVGNGTLGQVVRSPSGRLSTRQCARIALQVAVGMAYLHSQSPAIVHFDLKPENVLIQGEGENMVAKIADFGLSKRKHGKYVSCPTPRGTLPYMAPEVFFGNGEVSEKVDVYSMGVTLWQLHTGEEPFMRLSNHEILMGLESGTLHLPIPSTCDPEWRSLVETCMDPNPDNRPSFREIILQLQAILVQEQWTAKKSCSCAEVAVAAAVAVLDSDTIGRTLPRLYEVLLSPATPAAPAAPAAPPLPLRAFGETSGEVSRVCGLPPVPSLITLSGDNESSISTAPGTAGNPLVLGDSFSPARAPAMGVGLVAGGPGADATHIGAVAQCSPLRGVVKVRKTEVSNEEKIGEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFI3</accession>
    <name>A0ABQ5SFI3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="647"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="656"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="746" mass="77744" checksum="8C96E51A43786100" modified="2025-10-08" version="1">MAAGGGVQFLQSNVEDGAKGVAAYKPQNTEVIRKMLREQQRECQALHANPLFKEFRTPGSVRAKKFGKGYAVIEDAGMPQPFADPKSIEYEGQARMFELIKNTAVKEEKVRQGVWASFGAPYVPLGDPKELEKSLPELNRDKMGSRDGQRNGPRRKNSKQSSQHQQAAATVTTAPGGGQPAAAGGSTSIGGGGSRRRRAGSSANAAADARPGSPGSAGYDSDSLPGYGSSADADSHLSPAEAAIRAATREAEDTVNKQKSVMRFSVNDRIEASFPASVLTSANVRAGRARASVGAFVNRREAEDEVTDPHRAYGQPLTVRRQLMPLPPAARRIINGGGGGVEESVGVGEGSTFFLTQQGGQAAQDEERRRTSLENGAIASTSAPYDGTAGVSNGGSGLVPGGQPPGGADGTFMPLVEPVVALRGDESNPVYKWHNDFMAVRQSGRAQLQASLFARAQGRASVRTEPSNAAGLSTTLFGVLNLTQGRMPTFASGGKVVSPYARLEAYQAAQEAARRNTKQKAVQALDPSEIRILQRFYDQLCALVEVQRMSDPLCLMVVSKVKSLLEAGVYLHKPMLVAVLEHVASFAKGAGMMRYNKYLLAVLTFITKCVGLDVADLEDAVEQQGVSMVVYGTPVVPSKVTSDIVAALGGPDDEGTGSSTASGAKASGGGAPGRTRSARAHVKHGGSGGTVQAPISIPVGTTGEGTNGEGTQGDAAPKTPEPLMPAEMLDSLAAALTNGGDVVGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH04</accession>
    <name>A0ABQ5SH04_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_013812</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI69237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="110" mass="12619" checksum="F97D1AD3D872F3A3" modified="2025-10-08" version="1">MLGASRDRKGFSRDKVDIEAMEQENDRDLDHLADRVALLKNVTHGINKEVDDQHKLLDNMDGSFMSVRGLMGAVTDKFRLVYNDKSNKRLIYGVVGVATVLFFVWYLTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH73</accession>
    <name>A0ABQ5SH73_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="3">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_013241</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Host nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TlyA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004538">
        <property type="entry name" value="Hemolysin_A/TlyA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00478">
        <property type="entry name" value="tly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF35">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-1048">Host nucleus</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="14">
        <location>
            <begin position="131"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="92"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="555"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1214"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="555"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI68749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1350" mass="144185" checksum="D7756E816A3D565A" modified="2025-10-08" version="1">MLQTVRSLDRGGHGRAWSALYAQSRFLAKQFVKVNIQQRNSKVLCLSVGHHPAGLPTASTGTPPLTRPRAAAHRCRATEYCNSNSFHDESQCGANSNPAAELPPSPAASPSAACTLASPNPSTKRGPKQRLRLDEYCMQLAPQYSRNVIQSFILQGKVRVGDKVVTKAGTPVVVGSAGPGVVIDAEEPKYVCRAGLKLEKALAHWGLDVRGLTALDSGQSTGGFTDCLLQHGAARVYGIDVGFNQLSDKIRQDERVVVMEKFNLRHLGPQHLACKVDIATLDLSFISVLLVIGAVTRVLRPDRGRLVVLIKPQFEAGKKHIRAGGIVRDPKVHAEVLDRVTRGILAHGYELVGTTESPIKGDKAGNTEFLAYFIRRPELAEAAAVEVAEAETEAEIHDYCPYLYVPSPIRVYPIDGNGGSSDSGGPGATAPAEWRTGELEALRSHWNSRLPRESRLVSISSVVTRPVMYFRPAGHTGAPYLRLTLRPGASSKRAAAALEGLMSGQEGALLERQLGMRFVDKALHEAEVKLLMRFLCDSALSAGAWFYLPGGPIPTPTPTPTPTPTPTPTPTPTPTPPWAGGGGRAPLAESSGLTHPPYYIRVAPSARISTCGVEVEAPWRALQSLTPDATQLSDPDWCPEIVRRAGAVVGPGAAAAAAVAPMDPRLAAAVLAAHDGRITAMTIMTIDVLAVPYDTTNRVPVTAKDDPVTCIGCDVRMYGTAVGQQAAAARADGDGSRDAVGPYRVVFLLAGTDLTHGSPFPDVALQGAELRCFDSERDMLMAWKEWVLLVDPDIINTFQVRDTLGAIRDRFERLRLEGGGVLVSRLMAMHSGGMAATASPLTVKSVVMYSVAWVKRQGRMSSNSNQETFQADIRGRVVVDVLRQVLTSQTLATFSLVDCCQSLLGETLEVLGPFHLGRLAAEAQGRQALPAPPPAAPLQAAATAAVCIASYILRRVDVVVRLTSQLATLPEAFEMARVTGLTIPQVMYNAQMIRTWSLLHRYAHRQNYIIGGRQEAGNLIESPFLMHPIENHTAGLYRTPVATLDFASLYPSLYRAYNLCYTTLVHPDDVDTVGRERCGFTPTGHAFVKPEVRQGILPTILAALMAARAATRAKLKATRYDAAASAVLDSRQKALKITANALYGFTGAAASPLQCLPLADSCLAYGAAACRAAIQTITNALEEATVAAASTPPAAATTVANTGVDEGNATLTSKPALTLGAPAPSSGRELGDSEPSPLGLAVAAAAAGGRVIYAQTDSVFVHFPRASPKEAIELGKKAAALVTSSLPYPIELKYEKVMAPFLLLHVNRYAGRRMETEEDAASPEARGSLLVGLGQLPYTVYHLPYTRECP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHA8</accession>
    <name>A0ABQ5SHA8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013732</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053325">
        <property type="entry name" value="H3-Acetyl_Activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35706:SF1">
        <property type="entry name" value="EMBRYOGENESIS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35706">
        <property type="entry name" value="F14O23.11 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="16873" checksum="351A1168849C0B21" modified="2025-10-08" version="1">MYRRAATIACQLASRYINSLFEPGVISRAAVNASPTCSSIVCSTRLRPTLQSGLRTFSTFEVQNEMDAVNELFVAAREEIEFAKEEAETVYFNESVQAARRAVEACMSRWEGLLAALPDEERNRVMRSMGLKIAQLQAEYDEVSKLHLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHR2</accession>
    <name>A0ABQ5SHR2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="274" mass="25417" checksum="6C1068A1C41C7672" modified="2025-10-08" version="1" fragment="single">TANAVAQAVSQAATSSPGTTANAVAQALAQAQASGNANAVAQALAQAAAQGGSSANAVAQGVAQALGAGQAQAAAKAISVAAGGGSSSAQAASQALASGLGAGGGEATATAQAAAQAYSQGNGSAVAQALANAISQASGAGKAQAVGRAIAQALTSGDQGQASAFSAALANATASGCGAVAQALSEARASAASSGKSQALAKAVASSTARCLYPTCDPFFTPTDCCGTDNSASISAGKCGCLGGLCRYTLVRSDPPTWQSAFGALPGQDICVCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHY7</accession>
    <name>A0ABQ5SHY7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Senescence domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF18">
        <property type="entry name" value="ORC1-LIKE AAA ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="456"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="888"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="464"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="589"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="904"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="962" mass="96507" checksum="16A70AC30FEC291B" modified="2025-10-08" version="1">MAAAAPYTALTDSSDGRSRGRQQRPGPGSTFQSLTSPQRPQIARQGQAQPYSGGRTMPMYIASPEAELTDALHQLELCAAAGRAAVAAAEDDYGSQYIVRGSTRPHGRSPMTVAGPRAIAIPVLSPVASSYGSYGSGGRYLHEEGMAEDDVEGSSDAGSSRNSNNEHDEDGLGATADSSGTASGAGLGSRYPDKVCVEENPGAGLIVIHYPAGDTVSRRGGLNMRAPITADGHPPGAAQANNQHDLDAMRRWDMQPVNEVAGGRSCRGRSIALLPGPALPSIPAAEELATSAASDQPSITPPPPPPPPPPPPPVANGAAGAAAASASSIQDGSFLLPEAAVTAPAGLFPGTTEPSRQTTVVPALACASAAMLRLPNEVSYTGRVRRLQQPETAGKDLSGVWAEAAIAAATARPAAAARRRQSGHVTRVPGPGAGGSLVAPAAEYPLPPLWRRESHAERTEARLAAEAAAAEAAAEAAEVPGGGGQEKTESGGQRIAVRVGGLSSAQTTAAQRVAGQNKGGGPGGKRAEPITQWGGVQIGRTVAVAVPGLPAANASTSTCGVTITGAQPARLVVLPATSVAASAAAPAAPAPPIATQSAPSASTAIGSAAPSGSLDVRAESGVHQHAAVLQRSSGGEVRLDGDDDGDGGPLLDRFGMLSDEEGDEEEDGDGMMDETGVGDDEADGASHKSTQEAGGVGLRGQCKTVQGPRREIALGDSSSDEESPWGVEDADEMGYPLRRPGGGAGPAASAGRSAPDAAGANDAAARQNVDTSMRLVNTAGLVARCNGSSSTVGGAGGGMGTVSSRGALLTAEAVTYLSTAAAGHSAIRLTGQSVVTATNTSSGGAGGGVVSLRQVYGLAAERERVIDPRSIPPLPLTPPPAWLVRPHVSGHSAGGCQEAHGNEAPSAGAGASVSPANGERRQTIGRVEAALSQAETRASVNIVRHDSRTFYFSEHPSYPASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIU7</accession>
    <name>A0ABQ5SIU7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014602</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1028"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1097"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1182"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="736"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1247" mass="129858" checksum="A3E4A89C855EEB53" modified="2025-10-08" version="1">MNRLSAGCEAGRNSWIKSKVFLSSVSNTGDERGTIFRQAYWHLVFVLLQWFANISNSAVSSASLLLRRVQGVTQSSSDGLCRTAQHANSAYIICKPTAGLSCLQRQLSVAAKPVGPLASSDAAPQASETTITTTTASDLLECKSAPRKTFSFSSWRVVPVNFPSTPLGKLHNTIQPVPTGASHGLIHGQHEIARSILSCFATRPFVLSSLMLDNGAQHTVTDSYLPVERLRSMQFKISGREPEDLPTHFTEWLCALAGACGCLLSAVYIRKGCIELTLDIENAGMVPERQEGEEGQLEGRTLRLNAAASAVPTLPSAAATYGASGDGTTGSRETAATNSPKATQGQQHQQPKQQQRGVIRIGSGNENRFDLGGTEGAMALAQRLHDALGGGVGSGGMTEVRSRAAPVRRPSDGRDVNGASNLATGVAVPSLPQQQQQPASVVRTVAFWQSSFVPPLSYPAISRVRPRVIVVQAGRQVPAHRHGVSSTPGWDRGAAGVGAASVGAPMATLHHAGMAAVAAASHERRRESPRPITLLVEISAPPDVRLAVLGGAVGTPAMASHPVSASTDSIGASPAMPASVASIPIDTPDIELLVRCSGRYIPIDISLAPSQGGVATGDCDGEASAPDAEYISGGGGGIGSTVMRSYRITLLESPIVTGGVMLLDLRWQGRPAQVVPVVLLDEQDEQLQMELAAVAATWTGPPHELDGLLYDVGVWLRCMALRRAARTATQAMLGVGRSSTQAQTRGPGPAMSAAVASGRGCSPAARGEPPFLPVLGASLLQFAHAHGLIGMARRLQVDMRDCGYTAPFRITSMHHVAAAVEAEAAAGTVAGDGHSLSPPEATRQGPSALPELRCGMDAAARGRTTPTRPVVEVGIAPSPPSVTASGAVNRVLASESPSAVATLGSVDLHPMQRSASPAATVVTVGQWWFIWEVISLPPSVQRRRGSGPTLVEEESYLPTTFEEPLGAAVYCLFHLLDMLGLLELLIHLALTTHQQRGNSSIPAGAVVDTSHGGGGVGAATAALSHGTLMLLVVLTSLPVILNFSLQLLLPTTTMRRVAPHAKLVWHACLVARRVVLLWGGLPSIYPRSQQGYGLGPGVLVMEGVVVPAACLLSPWAALLLAALKLPLYVLAGHTSAIGKAADVAAAATTAEVCMSRGHCVATAPSRWHGDAGGGGALLVESVFRALVVTTMALLTTLACHVSLRMRTRKLGQVDAGKGVRRADGVQAEGKVVATAAVEGVAAARTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIW1</accession>
    <name>A0ABQ5SIW1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13214">
        <property type="entry name" value="PH-GRAM_WBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044852">
        <property type="entry name" value="WBP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31606">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 2, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31606:SF1">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 2, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="216" mass="23967" checksum="BE801C691235A1F5" modified="2025-10-08" version="1">MSANPALVRAPHGELCPAPFPNEVFVLRRDGVQCEIQGVQTRVKGWQVRGALYLSNVRLVFVAMNADESGLLAFDFPLCYIRNDKLNQPIFGCNNLSGQVWPAVQHGGPAGELPPHDFKIYFKEGGIGTFYHLYYTLSERAKKAFDEANGRNNNSDANPPDYVADLVSRAFVDPNDPSTVYLAQQPVDESQRLASAPRYAANYGEDEVYEPMGLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIY2</accession>
    <name>A0ABQ5SIY2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="272" mass="28596" checksum="054740409F4F48F7" modified="2025-10-08" version="1">MMGKQRFKGDLKFFVSFDAPPDRVPPQASDVLSSLPGLAAVKAAVISGLQSRGECVSGAPTTPLTALADRIMTVADGDDGGAGGGGDGNSVVTRITELAMAMAALIVGSRGAATTLPSQQPPGDDEDENGGGGGDRGAGGSAAVAGMMARMCGFGGFCGVPWVLGALRAVHPSRRRIRTVHRDLPGWSSHARNHPVLTTHGSPHVHEWFYLQCWCPWCWPTASGNVRVFEATFRILRCISLPAFACPQVKRGPWCRVIGHMTLARIIAATVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJV7</accession>
    <name>A0ABQ5SJV7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014558</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="851"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="616"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="797"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="898"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="940"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1029"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1068"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1140"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1211"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1375"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1560"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1689"/>
            <end position="1730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1744"/>
            <end position="1769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1807"/>
            <end position="1966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1992"/>
            <end position="2068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="898"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="910"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="968"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1217"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1244"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1328"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1404"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1457"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1479"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1569"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1600"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1612"/>
            <end position="1632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1747"/>
            <end position="1759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1808"/>
            <end position="1818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1873"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1917"/>
            <end position="1944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2018"/>
            <end position="2031"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2193" mass="223940" checksum="A592960D6AB70915" modified="2025-10-08" version="1" fragment="single">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</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK00</accession>
    <name>A0ABQ5SK00_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DEAD/DEAH box helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014504</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17923">
        <property type="entry name" value="DEXHc_Hrq1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18797">
        <property type="entry name" value="SF2_C_Hrq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055227">
        <property type="entry name" value="HRQ1_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957:SF3">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="MZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22982">
        <property type="entry name" value="WHD_HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="1093"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="1656"/>
            <end position="1812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="228"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="285"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="360"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="688"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="911"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1037"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1442"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2275"/>
            <end position="2294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="360"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="921"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1041"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1061"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1456"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1493"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2282"/>
            <end position="2294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2294" mass="243020" checksum="4C47C70350C866AF" modified="2025-10-08" version="1">MPAVAPRAPHCYGPGAVGYLGVTMQKAMEECSVPASMITAKAVSTSAPVAGRLVQEPVAVAGLAPTGRARGAAAVAVGTSFGVCTSGRTAGLVRTSACAAEVGAARDRMIESEGPAGDAVSPVLESAVSNQGIREPPIAPSISISLPIPPQQLQQQTSSQLPVERERIHVMVGRVNVAAAAPSHDLFDELKAELEAATLRSDEVAGRQREEPADTAAVLAAKLAETAGSFPSASKAAADSRHVRKKAKQANLPPPQSQLPLQPFKSVLGGSKHKDVVRDRINEGLQTEQQQQQQQQKQSSQRRGEATPSQTAPTFAGDSAVPQTAGRRPRRAAAEEAINGLAGTYRQGCSPPRWREKLANNERDCESDDGGRGRGLGYGRTAHRGEERKDNTDAEWSAEEALHSDDSSSHSPGKQGSGAYGDGDGVGASMSQRRRRQRSRCGESAEAGKLKRRRGCSAAVVVSGIDASGAGDGTITGEAVPGVSNGTSGALSGMCGHRPRRPKVVYPRSGPLFQRAVADCVLPLPPSLERLQQIFRGVNALYTFLLGQQVQMTWDNSCEALRSQMPNLNITLDDLLLAAALVPEVLMTKIVTQGSVAADATCFKQSQHGAEKASAAGGCGSGGGEGSLRTLLAAQDWRTDLVDRAVHLDRLAEADGTAAGTTQGTSGGVLVELIDPRRASPLLYEEAPAAEAPAAEKPGDCGDGRGTGRGADQAKELVGDGDGGDKGVGTSSPEKVVKRRQGERRRRAYLARPVAPPVILEDGRRLRFATNVITRRLAAFRSGLVSVLQHLHADFVNRLAPTERSGTRSGIATIPANIDSAGNHDSTYSSETAPVTDAMAARPSQATLMAAAAVPACTAVAAATAANTAVDPLPGIPPWDPAARRDWHPLFSAFLRRDGITFQQLRSAAAELRARRETRAQNTAGRSGSQPRHGTAPRPPTLLKQYEPCVSSAPLTPLGLLHHLQELPWYKGQVVHVEVLPARPAAVARPTAPLASPVLRALKSLGINEGRLYTHQVDAVDALLGYDRLGFNCAQQVPYPHHPHHHPHHHGHQQQHRQEVEQQQQQEQQQQDGVPLPGQSGVGPGPAPGLLGPYPRSRGRHVVVATSTASGKSLCYMVPLMQALSEDPDACALLMFPTKALAQDQLRAIRGLIEAAFGDHEGAAMPAVEVYDGDTSMSERPDIRQRAQVVLTNPDMLHATVLPGHRQFSRLLSKLTLVVVDEGHAYGGVFGCHTAMVLRRLRRLCNHVYGTDPRFVMTSATVANPLQLASRLLGVDQTEITLVGPVRDGSPCALRRFVLWNPPLTERALKAAEVKTAVVAAAARAGSRMTRTEARAAARISRNAERARQRQLLQEARAARVRASQLATERLAGGAAGAAAQEEMLQLAAAAQPLQQLKEALFGGSPRMRRAAERRLAEVQTATVAAAAGISTASAAAHLAVGGSGSGAVAGTPEPVKLKEGEPDETKYPVRPVGAISPGRATKPVPMPPSRQAGPSNPGSGTTGQLELPPGMMQDVAAMAAQVRAALSAAVDVNIEGNRRQISSLVSLRPVVSTSDNTAPVSNPVAAVAAASAVDSMASEIPPTAQASRPLILRTSGLAAAAAALGLPLPEGGGAGAADSRVVRELLSRLPGPEWREKHGAAGTPLEHRRDSPIVEMALLLAECVQHGLRTIAFCKSRKLCELVMAYTRETLVACAPGYEGRVKVYRAGYSPAERRAMEADIHSGRLLALAATNALELGVDVGGLDVTLHLGFPGSVASLRQQAGRAGRREQESLSLVVGFDGPLDQHFMRHPEHLFGRPVESVQIDVHNPDVLQQHLLCAALEHPLLLDQDLPFFGCRMPDIVARVLAASLICRHPGAPFGSTSGGGSQEWGGIPAGGNGGRGGGGGGWDDWLSVPLIYSGPKDNPASAIYLRSIDPDRFVIWDETTRMPLEEIEANMAFYEVYDGAVYMFQGRPYLCRQLNLSERVAIVRPADVKYYTKVKDYTDVYVTGGHVAYPAPTPALHRVMGAPVSGPDPAGGPGSNSSHRAAAAVAAPVLTEVEKRAPCLASAVPNEAASVTATATAKVGTDTSLLGTAIESMSATTASCESALVVVRYLGFHRVWQGSNRLFDTVDLFLPDVIYETQAAYVRVPPSARHECSARNLSFREGCHAANHALLNVVPLFLIANARDLGTECDNPYDARYRVERLLVYDKHRGGMGLSVAAAPLFPALLERAHRLVSECSCPYAKGCPQCVQHLECRNYNAVLSKTAAEVVLRHVLEQERAHVEVEATGRRGFRGTGRPERMDEKKGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKY0</accession>
    <name>A0ABQ5SKY0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015589</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025755">
        <property type="entry name" value="Ribos_uL4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14374">
        <property type="entry name" value="Ribos_L4_asso_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL4 C-terminal" evidence="4">
        <location>
            <begin position="275"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="410" mass="44880" checksum="6FF1E2FB12C4FFEF" modified="2025-10-08" version="1">MAASRPLVSVINVETGEAAEQTALPAVFTAPIRPDIVRTIHTNMAKNKRQAYAVFMKAGHQTAAESWGTGRAVSRIPRVPGGGTHRAGQGAFGNMCRGGRMFAPTKVWRRWHRKVNVNQKRYAVCSALAASALPSLVLARGHRIEQVPEVPLVLSDAVEGIKKTSKALEILKKVGALPDVEKSKDSKSLRAGKGKLRNRRYVMRKGPLVVFANDEGISKAFRNLPGVEVTSVDRLNLLQLAPGGHLGRFCIWSKAAFVKLDKIFGTTTVESEVKKGFKVPRSVMANSDLARIINSDEIQSVLKPAKAAGPKHAPLKKNPLRNLGALLKLNPYAKVARRIEIKRSARKAVVRAEKLEKIAKGIPTGAKKDKAVKSIGKAFYKKMLVESDYTGEDYDQFSRWITVQKQTKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKZ3</accession>
    <name>A0ABQ5SKZ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RPB5 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014941</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1340.10">
        <property type="entry name" value="RNA polymerase, Rpb5, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005571">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036710">
        <property type="entry name" value="RNA_pol_Rpb5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03871">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000747">
        <property type="entry name" value="RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53036">
        <property type="entry name" value="Eukaryotic RPB5 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb5 N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="5">
        <location>
            <begin position="129"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="23846" checksum="41129D4AC1C8A0A9" modified="2025-10-08" version="1">MDNLTRLWRVRRTCLQMLNDRGYLVSQEEINTSKDQFRDRFGENPRKDDLTILVPKQDDPTEQVFVFFPEEQKVGVKAIKLLAERMKDEKVNRAIMVTAVKFTPFAKSALEDMRPKYHIEHFLESELLINITEHILVPEHRILSPEEKRTLLDRYKIKETQLPRIQVSDAVARYLGLQRGQVVRIVRPSETAGRYVTYRFCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL63</accession>
    <name>A0ABQ5SL63_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="161" mass="17106" checksum="ED3F049929B46B6F" modified="2025-10-08" version="1" fragment="single">QVSDLISQLEKLRSGAAMMEARKGGLSGASTDLQGSSITAKFAIIPAASCPAMGATSPPSAVEVADAGAASIFINKAAQQPQELYQPLELTQPPKPHPKTPERRLPPEARNWGLQDCRSWAGEQHWSSWAEVQAFTKTLEPTLAEAIREASKSGGVHVALH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLR9</accession>
    <name>A0ABQ5SLR9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rubisco LSMT substrate-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016032</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF9">
        <property type="entry name" value="RUBISCO METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rubisco LSMT substrate-binding" evidence="4">
        <location>
            <begin position="323"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="454" mass="49979" checksum="2E47F5B43FDA1D90" modified="2025-10-08" version="1" fragment="single">MLALRVHGPMLGGAMHGGYRARSRSGRRVSGIPRSTAVHTASELVDWLKENGAKIDAVEVKTLDIPSAGRPLDVVVAGRSLGAGEVALSIPEHLCVTLDRIFESEFVAELLTTDKLSELACLALYLMYEKKMKKKSFWYPYIKELDKQQARGPQAVESPLLWADQELDSLLKGSPLLPAVRQRQAGIRKEYEALDTVWFMAGSLFNKYPFDLPTETFSFELFQQAFAAVQASIVHLQGVPIAKRFALVPLGPPLMAYSSTSKNMITYDEENRAVRLVVSGPVEAGRPVAAWCGPQPNNRLLLNYGIVDEHNPFDKLQFTFTLPTSDPLFTAKRAVLTDAGLATQQNFDVSVSRPLPPLLLPYMMVALATTPEQVAAVSFAEKTPPGHDRELEEAALAALMSYVQRRTAAYAHPLWRDLEIINDPSSSPRQKVAARLTKIEKSILAAAVEALSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLS6</accession>
    <name>A0ABQ5SLS6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histidine kinase</fullName>
            <ecNumber evidence="4">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_015764</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal/bacterial/fungal opsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001425">
        <property type="entry name" value="Arc/bac/fun_rhodopsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
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    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF72">
        <property type="entry name" value="OSMOSENSING HISTIDINE PROTEIN KINASE SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01036">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01021">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="14">
        <location>
            <begin position="347"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="15">
        <location>
            <begin position="994"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="16">
        <location>
            <begin position="1166"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="584"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="628"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="748"/>
            <end position="773"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="922"/>
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            <begin position="1380"/>
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        <location>
            <begin position="1469"/>
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        <location>
            <begin position="1520"/>
            <end position="1543"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1775"/>
            <end position="1825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1850"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1936"/>
            <end position="1965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="600"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="651"/>
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        </location>
    </feature>
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        <location>
            <begin position="1380"/>
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        </location>
    </feature>
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        <location>
            <begin position="1393"/>
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        <location>
            <begin position="1438"/>
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        <location>
            <begin position="1521"/>
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        <location>
            <begin position="1790"/>
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        </location>
    </feature>
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        <location>
            <begin position="1803"/>
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        <location>
            <begin position="1863"/>
            <end position="1872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1955"/>
            <end position="1965"/>
        </location>
    </feature>
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        <location>
            <position position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008130"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI70739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2188" mass="232337" checksum="EA5CC67159D74AE0" modified="2025-10-08" version="1">MYFHIKKGPGRRTGSMLQRGGSTIQRQRENVPAPYVAQPSLDDNCEDSEAFHGWVKTWAWTSACVFVYIAARWILDACIPGDPLASAELFKQVPLIVYQMSSTAFFTALVLNLTSLLFEDNATKRQLALLSCAIKGAACHTDMLLVTGRASAVYDAFGGIVIPQRYAQWLVTTPTMVYILSKISDFTPLQTATAICLDVIMVISGLMANFAPPYINWMMFSVSMLSFIGVLYMMGLMVYSAVKAHSSHTARRSLLFIYMCTLLIWSMFPVAWALHALRSATPHGEYLNVFANFMAKVLFSSSIMYGNYMTIAQRRLLAQQAAENAHRVQIIHELKDAVMRKDQFMSLMSHELRTPLNGIIQLSDALVRGAGGEMNVKGQHFVKTIKNSSNHLLNIINDILDVAALKEGKLNIKHELCSLAKAVDHVVDIVAPLARKDVVIERFVDPHTPFIIADFSRVIQILYNLAGNALKFTHQGRVFVRVAPSADGRAVTLQVADTGIGIPTDRINGIWGAFEQVDMSVTRKYGGTGLGLSIVKQLVEAHEGKIEVQSAEGHGTTFTVTLPVLQATTRPSLEVQVTESLTKCGHTVPHEPACRRRSQRLASTSGRNPSSLEETISQLARGTRRHANDIASPKGDSSDASGNISGSGNGPEREDVLYRRRTQELERENLMGELQRRTEHKRSMEEAQLASRQLVLSGPERQAERDSRSLERLELALGRGAPSHAAAPSVAAGGRGSCSSGGFATAAHRSISGGGSLRRRSDTEDSTPLRGLAGAGGSVGSFQAWRGDAAMVSRQSQSPDMREAMATRVSGCSGYGGSVTVSAGPNSLADAYEQILIARASADSFSSNQLLVRKLSAASIGGNAKPTMGREALRISTYGRAPGGCGSSSAHAHSGPISATSSHLLLKALDSDLYRTGRESFDDASSIGVDSESDAPRSGRRYQKGSASTCRSSKLVTNAAPLNGKNAAAASVGTPPPGLTLDKLPYSNMYGIMQILSVDDEEVNQIVLEEILTSTGYHFARCMDGLEALEWLCASETMPDLILLDCMMPNMSGHEFCATLRKVVPGNVLPVIMVSAKSDEDNIVEGLRSGSNDFVRKPYQREELLARIETQLRLKSDSWWLAELVNNVDGRETESMKLLKNILPESIIARMQQGQKFVADSHAHVVILFSDIVGFTSLSSKLPTAEVFLMLSNMFTAFDKLTDRFSVYKVETIGDAYMVAAGHDEDDDKALKGTPLMRVLGFAKAMLDVVRNITAPNGERMRIRIGVHCGPAFAGVIGMKCPRYCFLGDTVNTASRMESTGFPMCIHVSENVYQHHPNPEGELLEVGEREVKGKGRMRTYLVRVGAWEVALRDLAARQQAVAVASLQQQQQQELVIAQSTGQRQRQQAPDQGAASVSSCASDSSGLPQPPVQPSPFHVLPNGNIAASGFRPAGQLTLSPSPSGCDGSSNSSNDHNNNASRFVHLLSTVTEETSLPPSPPPPASAAVGLPAPQLRLPSFSEHHGAAALAAGGGSVGGLLRFSTDSASSHGPDTSPAGQRPRLDVPRSTFGSEAYYYDESSENGAASTLNGMISASVALRSCSGSLTGLLSLPTSAVTAPSSALQLAPGGHHPGGGGGGVAAVGGGNHKDQQARDNFLVTAAKLGLGVSGAVMAGAPPSLPREGIQGALNRSGLAPAADCTAGSAEAALEGKYFGCLRQVASPGCKGPKEDGSALIDYLEQRIASLGTQLTVEASKRHQLQADLDAERRRAVAALQHVALLQHQAAAVAAATSSTATCSSHPSATEGAAPIAKPPNCNPQPQPYPLQHLHARGNPSSNSSGGDNAVPIVRRLQPPRQPRQGRNQHLFLPVHGKPQQQQEEEEHQQEEKGQDDPHSGSSESPDAEVVIVGPADDDVDSASVPGGAARAGGDTIPEPAAELPPASVLVPDPPRFPLLLPSPEAGTSSVLTREKQPRSRAGGGGGGFGASGMPGSPFILADIEWHRQLQEGGGGTAEQVARCLPRRREPRLTTGALAAKPVIVDAAAATANPRRLYGGGFPLAPGGLFGLELKAAVVAAAAAAGGASGGGDCSTVQNSWLLPRYSLDLLFEELGLLPYLETFRREAIRLDQLLGMDVGQLERLGLRPLGYCLRVREAVMDLARGLLRACEEVAQLSQPPPSPNTSAAAALVPQAAAAAPAATAGGVDATVISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM81</accession>
    <name>A0ABQ5SM81_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein ENHANCED DISEASE RESISTANCE 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000102">
        <property type="protein sequence ID" value="GLI71097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045096">
        <property type="entry name" value="EDR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009769">
        <property type="entry name" value="EDR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12136">
        <property type="entry name" value="ENHANCED DISEASE RESISTANCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12136:SF41">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY (PH) AND LIPID-BINDING START DOMAINS-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07059">
        <property type="entry name" value="EDR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein ENHANCED DISEASE RESISTANCE 2 C-terminal" evidence="2">
        <location>
            <begin position="768"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1116"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="714"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1196" mass="125419" checksum="41F249766BE1DB5F" modified="2025-10-08" version="1">MASSEYGGPDDAEIPLEEADVYKETSNSNYVVIRRLRLFRSKFITFHNFEENDEDRKHWILTSQCDLSPRTAVEIREPCTKRVRPRGQWAITTLVKPKSIGTRGKSVRLYMFTLSWPTRWAAKGYTSFSFGFEDVVEARHWHMRLSDCLEAIRAEKAATSSSQSGILGISSSGQALTARLSTGNGGTGAAGSYGRSSNSILIAGSQAPQAPGAGVGPSTGMVKGGSMGRKRISGDGPSLGTDTPAVQMAGLTSAASSPEVPQRRQSASESAKRTLVYDSNSGTAATDTPPPPSSTDSEGSYEELEDPAVSGCVPCRQPRGTSRGDVYHTKQRWVPYKQVNGVAIYHHPQNAGDAEAGVGGEFMVSTVVRGSPRRCLNALTSNCGTTTILGPAIVQEVLQRRQAVDDKEVQRIVVQAPGLAGVICAPRELVVETVLKTDETVPTLMLMFNSCEVPARFVERKAGEQQQLLQAEEAETCRAASEGGQRSWWRRPIAARVRGGYTIAPLSENTLDQSQEALVTCIIKVDLGGVCGERSWFRLASDLAGWTDAFLDRMLMAVTLLRDEVEQARFVVQPLSMMASSSRAKDRDRDGLGSNADLSGIGAQPGRSGESHAMLCAVGGTAGMRRRLRLSTTAAAAAARAAVARGRSLIDAERISFVDGTAPEGAAAAEVPSVLGRGRISNSAEAAGTLAEAEEEEIGGEAGAAHRHEGAAGSGAAEAAAGETSDAGHGMGTLPHTYETLEEVLQRMRSLSFLDLSVWDNLHQPGKDAPFKIRGPTYLKDRKKIPAGFSKFILGSMDVIQQPPGTLHEHVARFIPAIRQSGAPFSVVVHLVIPGTPLLGIVATFVTDRHPATLGTPPAQPMEDEHDWEPFDFVLRKYMSGSTYARNHTLKLIPHIADGSWMIKQSVGTTPVILGKQLKTTYYETPQYLEIDIDISANNVASYVTGLVRGATRSLVIDMGFVLEGTTPWELPEALLGTLRLYHLDIRCAKLIDVSKEIPLRPANMAPPPPLRGHPYTTSVPTSAISGVMSTGATAVGEVSGLCSPGPGSGTGVATPAPAVAGAISVGSDGFGSAISHGNGNGNGGTGSTAAVAVATGVGAGQSSFTSKLTGVNASVGGGGGTSKRPASGFIGQGSAPANSPGSETSSNSGVGSRSVSAHQTPLPPRAFASVSASGVSTSNAAAAAAAVEATASLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNS5</accession>
    <name>A0ABQ5SNS5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2510 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="651" mass="66104" checksum="67A9D3FC9A027B28" modified="2025-10-08" version="1">MDSNYACYGGSGAPYPPPGPADPNIPTAYAPGASQFRQSPSWQSMDSNKGRTEQSIPAAQPPKPWYGEPPSWDQAASGHGITAGSGPQMPPPVYQPSPGPGPSLPVGPESISAPSDAESYYGSNRWTWDGRQWRPSTHTGQQYYGTAGGQQQGGVGNDGKGTQWTGWNGEMWVHSGTEHSGSFGAGAPGGCNDGSSQMLPPYYHHPPSGSGQEPPAQQPLLPPRDIFAAAEPYGQIVAYHPAHPASSQPPPQSGASRRWGSEGPGDSKGFRAPSYQPASSSSQWNHGAGGTHGGPRDVGFPPAPSKLTGKRPRPVLNSYTADPRNRCVARFQAVEVSGEAAAQAHKGLAHRGIGSYDRPGSPLVSKAAVEHALAKLTFWVQALPKGIKPDLAQGASIVMAATTAPKSATTGPPAGPPAPSGSGGAGASQQVRQVPTSPWSQAAQDHSPIPGLAAASGALSQPLHNTAPMPSAAQKDSSCAPAPAALQTEDLSRGGGPTAAPGSPPADDSQMASVTPAAAPTNAIGMKYSGHAAVESDNASAGAADAPAGDIAAVTTLAGRAAFVVNTAGEGGGGAPSSSRHTLPLELPRPQAHEQPRPQLQPSDALGRPLSSNLRVRRQGLMMSLEKRLLAQGSAAASSSPTASPPSAPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPA8</accession>
    <name>A0ABQ5SPA8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016683</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="118" mass="13163" checksum="61D03A797D3F94A0" modified="2025-10-08" version="1">MMPHRLAEETAFVTPKWATKAGDYTVMNQVSAVLQQWFRSGLCWPTQQRYKNGIEQDHPGSYLHTYGTVRAVLTGPGGRLPGRRVHVLNDSTTVQSIATKGSKGTHLQASGPQQWWTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPI5</accession>
    <name>A0ABQ5SPI5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016649</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17041">
        <property type="entry name" value="Ubl_WDR48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051246">
        <property type="entry name" value="WDR48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021772">
        <property type="entry name" value="WDR48/Bun107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862:SF14">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11816">
        <property type="entry name" value="DUF3337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="71"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="250"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="805" mass="86914" checksum="F764DF1033863D50" modified="2025-10-08" version="1">MYSNGAVPRARKERKVSVVFRHPNDEKHCAGVEAVLCPVVPNHKLLFTASRDSTIKRWDAAGPEAIMEASFDGHADWVNDMAIIGDLLITCSNDQTVRLWKAGSENGQHLHTLSYHSDYVTCLAAAPARSMVVSAGLRAEIFTLNVETGKHTQLYPRSQQSASPNSLSSASSVHAHVSVPSVGVLGSEDGPFTGGSVSNSPFSSSNLAKEQPGSVYALALNPTATVLAAGTSEALVRLLDPRSGKKITKLRGHLDTVRALLLNADGTTLLSGASDGTIKLWDIGMQRCVQTYTLHTDAVWSLLSPDDSFAVIYSAGRDRALYRTYTHTRTSELLAVEEYPVRALALDSDVGLDVAGRSSVGLWAATSASHMNLWVVPRDLPPGSAAGVDTGRAFSVARRSQGLPVNMRNRMSLEQNRQPIARLPEMVIPGTPGLVLHEVLTDKRRVLTQDMRGHVGLWDVLQGAQVASFGKVDFSSKIRELWEPHSVPSWFTADTRLGCLCITLEPTSAFMAEEYGVTLEYGNVSDDFKVNYGKLVLDCAFAKWRYKLAASLSMHLQGVPAGVGLQGTGAPGNLLHSQMQLQGGGLAQPGTLVAAGSGSGSGGLPEGSGAQPYYLDIWPKYWTCRALPAVICSRQDGTRWRRLISSFDGTEVEPAEVPTWVGDVVLRNANVTPKEAKCAFVLLPAEGSNLPSLPQSKLNAPRILQVHKVANYCYGKLQDQGVPLVVRPFYLRRPPYAPESPPESCKGGEPPALELTCNGMAVPYEMSLATIKKYIWKRSDDLIFHFRIRDPEHPAPAPTISGPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPM5</accession>
    <name>A0ABQ5SPM5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_016846</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051855">
        <property type="entry name" value="eIF2B_beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="164"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI71581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="525" mass="55866" checksum="D17EC8B1E84BE6CE" modified="2025-10-08" version="1">MSEKKEIDDIVEECCTVFRRRQIEGSLPCAKKTLDVLRLIVTNKKHSTAAALIDELRNVGYKMQTARPVELAIGNMVRRVLHIIREEAKQEEFDDVKPTLSQPSLQETTRNQSGGLLSQALKGRLQMPRGPSLSNLLDAGIDPASFNPSLAPIQESPADRLDVVTERGPDPEVDSQAGGLSRSAARQNKKTSQNWKGKHEVIESINELIDELDHIQGSITTQGVEHIHANEVILTMGMSDTTLLFLKEAAKKREFQVVVAEGAPRFDGHLMARRLADAGIQTTLIADSAVYAMMARANKVLVGAHAVLANGGVVAPVGLHMVALSARRHSIPFVVLVGLHKLSPLFPTDPDLLYNEFKGPGDVIDFDVVGDAFVQQQQLHNAAMAATDCSGLGAAAAAAAAAAGMIPASVGGCAISGASGGSPAGAVTALDSVSSTSFVLGGLNGTSPPSMAAAAAGGALRGDPLPGNVHVPTPLFDYVPPHLISLFITDMGGRTPSYVYRLLTEYYSRDDYFLSREVFGKDGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ86</accession>
    <name>A0ABQ5SQ86_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-structural maintenance of chromosomes element 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_016774</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the SMC5-SMC6 complex, that promotes sister chromatid alignment after DNA damage and facilitates double-stranded DNA breaks (DSBs) repair via homologous recombination between sister chromatids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the SMC5-SMC6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NSE4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027786">
        <property type="entry name" value="Nse4/EID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014854">
        <property type="entry name" value="Nse4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08743">
        <property type="entry name" value="Nse4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-structural maintenance of chromosome element 4 C-terminal" evidence="9">
        <location>
            <begin position="215"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="310"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="346" mass="39305" checksum="BCE3CF61C771554C" modified="2025-10-08" version="1">MAQVAGVFGISREPYRQLRNELREQKQNLYRNKEVLCDISTNRLDEAVKCLEQVRERGDALGDAFRKPKDVSAQAEIFRTLAGYGVVASNKLVQQQQGRGPIELVRAFRFGFVHTRDPQRDGESEPHAFCWQELAAATEHLRRQARGMHCVLGALDAKPKERKAPVQRQKRKEVAELQRPDVQEVIPAEDKKETDRNMEVMWAVIKAQADPMVLVAKLVCNQVSYGQTLENLFTLSFLVRDHRVGMKRVPGKGLFVWALDTKRADAASRQQQSKPPHQFVVTLHIDDWKKMCTRVAREDCLMPHRDYGIPDGVQPTGKRRQGHGACPSPNTRQRRAEKTGVGPGKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQM3</accession>
    <name>A0ABQ5SQM3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_017090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000120">
        <property type="protein sequence ID" value="GLI71768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="28097" checksum="7BA8240258B5D054" modified="2025-10-08" version="1" fragment="single">MLDGRTGLLCFDPLCNLFTPRRPLPSIDLSNHVVLFIPSSLREMSEQLAIYRRCRRQYPGTAACVLLPAFCRRKAEASLRGMRHVHRFDPGTVLFNGVDREGTLAPWPSTSYLVDVWYEAPDGGAVGYGPALSALTLSAGQLLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVAEVNALGLSPESSDCPQVTLADGGKHPIVGRITLSLSIGPLRVVTRPYVLQGLAETAPYIMGSSTLRQYRACIDMETATLHMCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLK7</accession>
    <name>A0ABQ5RLK7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_000248</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02173">
        <property type="entry name" value="ECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="14">
        <location>
            <begin position="80"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="14">
        <location>
            <begin position="284"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI58512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="448" mass="50091" checksum="D0811308D807EF00" modified="2025-10-08" version="1">MKIFSSLLDALKAKDAGFWACVAAAATTAVGIATYYATKSYEIYIPIQYQYRPGTISGWLAKALAKHSKSRRKRRPVRVYLDGCFDMMHYGHANALRQAKAVGDELVVGLINDAEILRCKGPPVMNEEERYTLVEAVKWVDEILRGVPYDLNPEFVHELFTKHRIDYIIHGDDPCLLPDGTDAYAHAKKLGRFKMVKRTEGVSTTDIVGRMLTCSRVNHFISDDEPHPLAKSFSLGTPREESGEEASCSDASTRTTLSKFLPTSRRLVQFSNGRVAPEGARIVYIDGAFDCFHPGHVKILQAAKAQGDFLLVGIHTDEEVQARRGPHLPIMNLHERSLSVLSCKYVDEVIIGSPCVMTDDLIKTFNISTVVRGSISETSMLGPVEPERYAFPRAEGIFCELRSPSDITARNIIHRIVDKRTAFEERNARKVKGEEAYYTGAKTYVQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMA5</accession>
    <name>A0ABQ5RMA5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_000149</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI58433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="119" mass="13423" checksum="9C5CE9C9C526E794" modified="2025-10-08" version="1" fragment="single">TGYTNKVDCWSVGVLAYELLVGQPPFAAPTAQETLRLIRTKRVEYPSWLSTESINFMSIVLVRDPTQRPSIAELLDHPWILKYSRREGGGLARTRNRIQQRRPTVDGGVLVRSMTAPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMS3</accession>
    <name>A0ABQ5RMS3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="140" mass="15069" checksum="DB66DBF495595F95" modified="2025-10-08" version="1">MPSASPFTITPPKACAPRKDGRLQASSGSIQGRRRQLSGQRQLGGLDHRDGVNGHPLRCKHATGVRGLSRPVQAPSILPNDAQLVYPPLHDVAALDRPFGRLLRDSSHLRLVYKAICAFNPNSCDLSNAHGSFDAVCLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMZ3</accession>
    <name>A0ABQ5RMZ3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000706</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="168" mass="17872" checksum="176375194C7C89A6" modified="2025-10-08" version="1">MFNSQTGFVSNFVEQDGCARTVIVLLLLTTVGCVSYTWGYYEGIANAQSNLMLDKLHEMQLLQMSAAHNGTGAQKHAIGHGMRLAEVQPGKPQDKGEAPDKPAADEVDKAATHAAAALEEAATGSHGRKASDHAAAQLRGSSSAAERDPSAVKRRQIRKAKPGPHEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN98</accession>
    <name>A0ABQ5RN98_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000827</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044715">
        <property type="entry name" value="WDR86-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489:SF11">
        <property type="entry name" value="WD REPEAT DOMAIN 86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="141"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="228"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="274"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="94"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="458" mass="48611" checksum="354DCC70D236A36D" modified="2025-10-08" version="1">MDDWSGEGRGSYRGRPSGGFGGGGGRYNDNYYRGRGGRAGGGYYDGSGPGGYYDGPQGARGGRGGSRGGYQDYYQAGSYQGGGGYDGGGYRGGGGRFNDGGSRRGFRGGRTWNNAGQKRQQQQQQQYNSNPPPNLALLKTIQAHTGAITCLSYDQATNALYSGSKDGKIKQWDCNSGQVVHEENLNGQVTAILFVQGFLFVAYVKGQGRDQDGIINVYNTAAGKTQMIPGHRGYINQLMVANNLLFSCGQDNSIRVWGMEGEAFVCKQILDKDKGGHTSPVHSFEMINGFLVSGDSSGTLKIWDSSTGSCTQTLPMAHAHIITSILQYGNNILSGSSDSTMKVWELTNPPMPGAVVKPEPIDSYFTDDGASRGGGRYRQGGVPNAILTMDGSPDNKGESMLAIATIKTGVSLYNVDQGMTLLGTLPRVDVVRAITNVPGAAIILGDDNGQVHIFSWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNM1</accession>
    <name>A0ABQ5RNM1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000974</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01190">
        <property type="entry name" value="RIBOSOMAL_L36E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="99" mass="11242" checksum="60E1135D19E19632" modified="2025-10-08" version="1">MVASGLAVGLNKGHVVTKREQALRPSRTKGRLSKRVKFVREVIREVAGLAPYEKRIMELLKVGKDKRALKVAKRKLGTHIRGKRKREELAGLMRKMAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNQ4</accession>
    <name>A0ABQ5RNQ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044690">
        <property type="entry name" value="CAS_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34209:SF1">
        <property type="entry name" value="CALCIUM SENSING RECEPTOR, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34209">
        <property type="entry name" value="RHODANESE/CELL CYCLE CONTROL PHOSPHATASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="243"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="338" mass="34604" checksum="A7A6CD1C1021C342" modified="2025-10-08" version="1" fragment="single">RQFLSLTRRMAQVQLMKGASRATMRPARSAVCAFAQQRTPAKSAVPKSLAAGLLASSSFLQSSAARAAEADQLDTTVETVVGAVKATGEVVKAGISTIQTGVKILKDGYDVAAPIAKQAVDTVAPVVVEAAKKTVELAQPAIDAAAPAISNTVSDVLESTGVDLSAVTNAAGTVGDVAGTAVSAATPVARGVAEALASSDPLTLGEYALGAVALYYLTPFVFGLFRGFAGEVTAAVALDTVVNDPSAVLIDVRAAREKEASGVPDVPGAASSKVLEVEFAALEDKKLRSQLKDPSFIEAQTTALQIASLRRIGTGSKVILLDRYGPQAEAVARELAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNX1</accession>
    <name>A0ABQ5RNX1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_000486</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005177">
        <property type="entry name" value="Kinase-pyrophosphorylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003742">
        <property type="entry name" value="PRK05339.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31756">
        <property type="entry name" value="PYRUVATE, PHOSPHATE DIKINASE REGULATORY PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31756:SF3">
        <property type="entry name" value="PYRUVATE, PHOSPHATE DIKINASE REGULATORY PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03618">
        <property type="entry name" value="Kinase-PPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI58732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="644" mass="69996" checksum="A9E4C1FDF952F905" modified="2025-10-08" version="1">MAHTAAGRQLHGASHAHPVISASLYRSLVTFCTTRRRARPPPWPRNASSGQFPACPRAQAKDDVQGIEPDVLAEPMAVAPRPLESLDDMAAFVERTRQEEENIRRRREEVELQLLRMRQQEAVRRQQAIKLRVASERANAAVLREQGGTTTLGSILVPVSAPPPELELPETGAVPAEGTMSAATSAVAATPTGASISASNSALGTTIPKKKLVLKKSKRMTAVAAAAGLHRVSSPPQQGSGAARRASNGVMAGGSDTGLALAGAPSGPSGSVGKALLSREELIEKRKEVSTATPGGCESSDEDCGFDPVIVPKPIFIISDCTGESAARTVRAALNQFEMRCQTQAPAQIMIYRFVESVDKMMSIIREAAKEDALVVYTLVDPKAVKIVQTACKLQNVRYVDLWTALLDTMEVHLNAVRSGVPASLTETRKPTASLTDEYFRMIEAVEYTRKMDDGAHPQEWVNADLVILGVSRCGKTPLSIYLGQRGYKVANLPLIPGCPVPRELNEIDQTRIVGLIIDPNVLATIRRNRVNIMGVSRAQAIDYAEVKKINTELDWARKLYNSHPDWPVIDVTLRGIEETAARILKMLNDRRGDASPKWVEAYHAKQHALAMTQSPSAAEESPYNIPEMIYGAHESTFLMQGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP06</accession>
    <name>A0ABQ5RP06_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="108" mass="11028" checksum="7DB939B21990DEDE" modified="2025-10-08" version="1" fragment="single">MATQELSVGARSPQVTVLSHNPLYNQRGPADPRVTWADTPPSLSGPLHTSPGRPPPTASPPASCSTNRGAKLRTSPDLDEGESLRQPGSSGACSPCAFPLPLGASAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPH1</accession>
    <name>A0ABQ5RPH1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_001273</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13250">
        <property type="entry name" value="PH_ACAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF160">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN EFFECTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="306"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="8">
        <location>
            <begin position="538"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="843"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="874"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="628"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="506"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="962" mass="99744" checksum="5A7AFEE7CD98915B" modified="2025-10-08" version="1">MVTQMPFSDLEDTPIFRIKVSELEAGCRRLRDRVTNLVGHYRRYRDALVALCKAQSAFVGGLAEFFSGSDGEEMGGGMAVNKYISTLGESTSYLDLLKIQIEFAADQISAEWLDDLLVAARDSHRTFERSSSDLEDVTAKCLALKKGTRREVLDKATAELAAARLVAEEARFDVARRLAAVEARRRYSFLQLLLDSVGAHHAALRSGSEMLGRLTPLGDTARGLVAEARAAEGEVQSRLSREAERCKAISEQAAALAASSLSFGDDSGHGPVQMTAAKSGAALAAEAALRVTRAAQAAGDPNPPVTVIRQGYLLKRSGGAAAGSGGGGKLVTGEWKRRFFVLDSTGRLFYYSQKDTLLNKIRGLESHQPATTCVNLLTSTIKMDDEADPALRFCFRVVSPTGTLALQAESEPDRAAWVAMLQVVISALLDAMASAPPAGIGAGGAAGAAITAGSLARPSSNYSVSFSGGGSGIPGSPGAAQGLVASSGASAFLQRQGSGGWEEMRGSNAGGSAPSAQAGSLAVGTGTGAATVDGTVVEAPLERLRRVAGNTHCCDCGAPNPDWASLNLGCLLCIECSGVHRQLGVHVSKIRSLTLDVRVWEPSILDLFSRLGNTAVNAVWEARLTQLQQHAQHQNPRHRSAGPAAGRVGGGPSSGRAGVDDTWVWCEEDEEDEGHGAYLATNLARKGLAASPASASGFGAGSSLWGRNSGGGNSAGSGEAWLLAKPHLRAPLAEKQRYIQAKYVGRVYVAPPPAHILSGPGGGPQQLGLLLWHAVEAGDAQAALQAFAWGADPAAHVRGPRAALLIQEMLEAAGGVAVGGNGSGSMPPRPHAGGGVLALTLPPLHLAAADGCLPLLEVLLQNGAPIDAVDGAGGGHTALHYALLADRYNVAKLLIRRGASLTVADSAGRRAWDLVVGVKGRVADEELFLMLNGAGHEAAPAAAVIFLPSQTQGVQPDGSYHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPQ2</accession>
    <name>A0ABQ5RPQ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_001277</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF254">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN SHAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="13">
        <location>
            <begin position="111"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI59396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="588" mass="65707" checksum="1EBF05A5FBDD9DEB" modified="2025-10-08" version="1">MRGNSQEGCIEAAQVAAGFRYGMETAADAEFDGAAGLYSGHDMAGLGIRIQAIESLPEPTRRYLHINILGKPLPTWMKWDNQPKINRFNYLMSRLHTVMTDTTSSVYAMAISHLMVLSIIISVLCFCLETIPAFQPERAPNVARAFTWVEGVTIQIFALDYLLRFVSTPDKVKFVVEPFNIIDLIAILPWYVVTFVGANFDGTTVFRVVRLLRVFRVLKLGGRYSKLLVVLHSLRKSMDMLGLMVFFVSLCIVFFSTLMYYAERGVYNAELGYYSRPNESQPKPSPFESIVSGFWWAIVTLMTVGYGDAFPLSAGGKFIASVTMICGVLTLALPISVIGATFSSNWAAHLEEEKNKPVLIKKVAAATSPVLLHLQRLLNRHLEDMEILISLNRNSELALEDASSQLHHTLKTTKKHLHTEAKADLRLRRNALRNRTNTAAITPPYHQQYGYLSMNHVKDRYREQLQVMSETAELLGRRACHVSRMEEVCAHLVDPELETIITRLSKKHADLGFLLDKHEMKPSPLALLEAELNDLKGYLGDCKAANAAVLSLTPATAAGAVMIEFRTSIPPRLTSPGQRLWSMGITAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPZ4</accession>
    <name>A0ABQ5RPZ4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903">
        <property type="entry name" value="GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903:SF1">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="884"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="908"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="924" mass="91490" checksum="E2522B5C77A1CB80" modified="2025-10-08" version="1" fragment="single">MVFCCFGRKLAAASLHQEFRDLSPDKASPGTRDSEVTEKIFAVSSGAQEQQAVQDTLSTLRYQLATVQGSWLARLHACVESVLAACGPVGPVCIFGASPCGSVLVLLGTAGSAGANVIMSPGHVLPVPQPWLSTSVGGCTGSVAVMAVGAVSSTSGGLVILGSPAGAPCPPGARCAGRSDVLMWQCSHLQGPAWEAHVLLPVAHPHNSLKVRTDSQPPQPEEAPQSAPDEWRQVLDAMAGEVVGVAGGGGGGGGLADPVGADKLRELPAALRAYLVPLRLGTDIIGALLMTAPTAAAPPPPLRPTLSRELLMGTAAAVAECCLGPHLKDIQRVTLAACELATAPDLQGLASCLSSAVTEALASELHVDFSVRLAIVPYVPGMRGTPQYGFLLTEQLAGTTTQPQLPHVTGGIHQNNVLGGNVAGSDLLSRLPMPHGPVSRAATRSLTQQAFAERLFAAKVSGGFPSGGAPCVDPFSGTCTTGQPSPPQLPLAGLVAGGAGGAMASGAHSFHLTGACGRRLGGDLPGRLWKARPFCLESTLLASLAAKVAKSATSAANGVSCPPAAVTGSGIISPTASISHCPLDDGGASSGVPSPMPRCVAHAHTITGGYSTYMTIGSSLVTQNCGGGGGGGGGGGGGGGAGLRVYRMGGTVVPHVHAYVNDTDQPSADVVLLLRTAVTGLGMSTPGLRGGCGGGGGSYPALAGSGTGAVGSGPGGGPVSLVVVMGSVALVTPGDANRGAAMATKAADSYGEDAVSTAAAARPLGRVTGNGMGLQSPSSSLWPASVLRTVGSIGGGGGGGGGGGGGSGGFDVCLALYLTSSDRLPPSLLFAARNRSQVLLELLLPAIGACLSESASAELQYLWDAVSGSAGGAATNGGMSALLSRAPSVPTGRLQPKGSPSAAEEGNMQPSHLSPHVQQSHQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPZ9</accession>
    <name>A0ABQ5RPZ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Basic proline-rich protein-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="23427" checksum="519D02A75D3CC699" modified="2025-10-08" version="1" fragment="single">PPPPPPPPPPPPPPPTPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQU0</accession>
    <name>A0ABQ5RQU0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="180" mass="19071" checksum="1A56121F69DC55C3" modified="2025-10-08" version="1" fragment="single">MTAIPPLSVSGDNSTGACGTGNATALGGTDRGGPGWTGLCQLAITPAGRGMGHIFLPGILNRAVGCGRVSRGARCYGLALRPVPRGVPADPCTEDESTDLLCARLTDFASGEITDVLVARTGGDMCRLAPPEDWLLRQWFGNDLESDEEEDGGAGLNAHRAKWEEVGEWEEVTEWEEDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR86</accession>
    <name>A0ABQ5RR86_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 30 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001885</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI60035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17310" checksum="27F56B3B3B0966AC" modified="2025-10-08" version="1">MIDTPLDDPIAEKLRQQKLVEQADYESARDLFGCVSTLKPLESYLPKTLKEFEDFAVEIASRHVVVHRESKHYKAFVKALMRAAVEPLGPEEVKDLETCLAGVRSDKAKKKKEEEEKKKGVKKTLNMGKQGISAGLDDYVYGDDEDNSDGDGFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRB7</accession>
    <name>A0ABQ5RRB7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sin1 middle CRIM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006886">
        <property type="entry name" value="RNA_pol_III_Rpc5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES III 80 KDA POLYPEPTIDE RNA POLYMERASE III SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04801">
        <property type="entry name" value="RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="45963" checksum="29C52973A08A7D9E" modified="2025-10-08" version="1">MDAMGVDDDDPVVRELDVYVCNEFAGSKTQLGLLQHPLRPPWRPYEYHKAKAMRFKPQAKRMEVDLPLDTESRNYNDVIEDIKKVNHFTIRSTLAEERINLAVGTIRGGKLLIAPLEFAVQLRPTMHHLNITSSAKDKNKEDSEDEEDDEPKLHAVEVQVQKRETERQAQARKSSYAYITQKEEEEPFVNLNVHGEDSDAAVKIWDLFMSAKDIDHGTSRLDRQVYLRAIVPPPASQANPSGLSAAPADAAGTNTSTVVVTGAPSVTGQLSEAARAALPTALRALFKEHTVCSLANVRFFLDNSPVGAAAKEAALLPDKVLHDAVLSLGNVASMHRVYVVTKTGDEKTDPLRKLVSELLEAKESFKRSEFNDLARTNNMVFTESQYNRVLKQWQQPREHCGEGSPVLAFLWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSE5</accession>
    <name>A0ABQ5RSE5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_001886</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Plastid</location>
            <location evidence="7">Chloroplast outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM50/omp85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI60036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.310">
        <property type="entry name" value="membrane protein fhac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF18">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="5" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="9">
        <location>
            <begin position="161"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="520" mass="55678" checksum="139B7D8B6ABA6EF6" modified="2025-10-08" version="1">MATAASAEGRPFAQSSDELSSEAQRKVEDSVSASQSVDYTALYESIKDKPCRVVQINQHVEGFNGRTFRTRPNVIERELEPIHNAQTLSEVHEQLDAAGRRLKQLGVFSTVEFLAHEEPLDDPSACTVDVHLEETNWYKLRAATYVEDSERTFELGASLTNALGAAEHLGANFEYGTENSHTASVSYKQPRAFGFPALVELRGSQLFRNNQKTSSYTEQLQGAVLGLKSLNGTHTVEYELGWRRLLDPSRSASRAVVAQMGDYLKSAVRYTALVDRRDMAAGGAAAAVGGAGWAVKASTEVAGLVPPTGSSTSRGGSGADSTLRFIRQQVDLAANVPMDETGAVVFSLGLSAGLLLPWGEGGMSRPSCIADRFFLGGPSSLRAFKFKGAGPSDARRPPAGRTEAETTQAATRRDALGGDAYTSIFASLMFKLPHPALKLLNLHGHAFVNGGNVMPVSGMGCTPTALLSEFGSTFRWSCGAGLVVPTPLGRFEANYCIVLAAQEHDRVRRGLQLGFAASNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSJ5</accession>
    <name>A0ABQ5RSJ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002684</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="114"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="150"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="319"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="523"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="661"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="951"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1071"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1157"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="677"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="968"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1091"/>
            <end position="1101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1257" mass="130493" checksum="E9619441DC892D84" modified="2025-10-08" version="1">MNDFEDAGTLLPYESDKRSELPILDFSRLAKLHEEFPLHHQHKMHVQQLPMHYSGQASPPAQPNKRMRRLRFARLAHLDSSTKARVSRSVQTAQVKSWLHAHGYVVKAEKLHPAVARVIEEWFQLVDEDNSRTLEHHELLAALKEAQIPCDDATINEMISLMDVNKDGVIDWEEFEVFMTEEFAAGKSLLSGEYLLPSGTSLNFGVMIGKLKRNRLLHDIMDDPNRRNRWAAIANNPDALGRELATMQEAAEVTSLTLEHLKRSEGRRVASNNPRTQLTTALIRTMDEGRNVPKTLEPGRNLAEKLRCGMSRRSCDMPQCDVGTPDSPNGQGHTPRGACPDPTTLPLTPNGSEGAFSACSSADTLMQVHLQAKRNHHNAPGGAAAAIDDGTTPLEAPCVSAGGGGFGGTGQHRSATQGFGCKSAAPQSAETVARYSASQQLCQSTTAPPTSLPQPQSQMSLGNVASLARWAEYEPRASEQHNKAPGSDMFAANTCLSTAATLPQPRKNDGTANAGWSGLMTSSSAPCPDAASAHGNTMYQSPNRHAAGPSRRGNGTATADGCRSVSPGHAPGPALVPHRPRNSVLQLASTPHYLVASTASSRAHIRPASRVISQVGLMAKELRGTPGRYFVEAPGLPPAVLTATRGQWSNTVAIAASTASGVEGGRMRDSGDGFGTGSQTASTSGGSTTTASNTASSATGAGAGGSATTSCHLADLRPASSPPQPFLQTQQSHGNGCGTLPFLTQAVHLSELQDVIIGDDTEASSTGGTVIVSQNARGMVIADGGSITGENPAAIATASPPDAALRAPIRSLATAAWEGPAPLQPLMASPRPQTASLPGDLAPACTDGSSSSRARTSRLKLQGSADSDAVGADIPTVNTVATALPAPQPSGLHDGPLPGAVGSGGVGAITAAASTGASKALKPTSIMMRISPGFPPYADVNTSRYSATGDVDVTTINGGGGKSDKTPRSHRRFSHGGPRRGSGGSGGTRESTGDGAPSVMHNSHVFGPNTQSHRVSVGAEGGVDALAPLMPPSAGTTARYWHFRSDDGESLYGGENKYGIDDRGHVWSLRRQSPHKGALLKGSDDNGAGRARTKARGRTRASRSGDGGDGPTSVAAARQRREAVSASAVATALLDALPLSSNTERVGFPGNNGALVSGAASARDSELGSGRSRSGGGGGSQPTDAGSSGTGRTGGSNIPGRASLALLGPPEGRPPSRARTPLNTRGDREDFIDSSWRAATASLLQLQLTHQLNDPCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTI2</accession>
    <name>A0ABQ5RTI2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="111" mass="12445" checksum="28C2708D454F183F" modified="2025-10-08" version="1" fragment="single">NTSSYPYRGQWDVRPGTLLDRSELTARAGVAFGLPPADAYQSGRRQGDEVRYVLERRRLMSEGRVRPIPALVDRPSLLVQPWKVQEAASRVREAIKGGVFYPMEGCRGDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU10</accession>
    <name>A0ABQ5RU10_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23808">
        <property type="entry name" value="UBCc_UBE2W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="9"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="160" mass="17872" checksum="22CBC0CA0C0D2F21" modified="2025-10-08" version="1">MATKTLSTVAQRRIQSELNEWLHSPPEGGVLESFEPMTSWVVLMQGPEGHGRLYADEVFRVKIEFGEQYPLDPPEVIFLAPAPIHPHIYSNGHICLDILYSGHNGGWSPALTMSKVVLSLRSMLASATEKRKPPGDAEYCARVGNRSPKLSKWVFEDDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU47</accession>
    <name>A0ABQ5RU47_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003042</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF360">
        <property type="entry name" value="ULTRAVIOLET-B RECEPTOR UVR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="200"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="202"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="264"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="316"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="372"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="418"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="470"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="524"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="665"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="757"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="960"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="766"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="776"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="791"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="848"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="971"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1000"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1018"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1041"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1066"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1079"/>
            <end position="1103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1103" mass="112465" checksum="D6BE8B96267CBC54" modified="2025-10-08" version="1">MSFSSFTATGIPPAVSAPETTAAKSWDISAALAPAPAPRCGGTDGVVNGNGLTAAVAAQPRQPTAQPSRQGCSSLSPTESQSSLHLGVLDFGQQQHQQQVQKQQLLAQQQVQLAAALPEQQQQQQKQIQLLQQQQHCAGFTDLQAYQANGSMPQGGSSSESMGSLAQYGTSSSAGQLMLAGPGSLCAVLPYYVRRPPIEAVVFGWGVSEDGQLGLAAARENVIAPKNTPQVVEALLGVQFSGRSFSRTPLVAGSRNTLALDAAGQVWSFGWNDRGTLGHGHRNRETKPKRIAALKGIRIVQVAIGGWHSLALSDRGTMFAWGGNEYGQCGYVLPGQRDITEPMPVDLGLGTNQRVKQISAGGMHSLALLEDGTVWQWGENWGDFSMRPVRTPRPVPGAVSVAAISCGAFHNLALTAGGRVLSWGMNDYGQLGNGSTTYSTAPRPVLDMDGVVVADIAAGGWHSCALSSAGEVWVWGRGEYGRLGLGDRSGSSKLRPQKVRGLEAHVVSQVAAGGSHTLALTSTARLFVWGRGAFGRLGLGDPERDCYHPVECALPGGTERWRVAAIGAGGRHSICLAIPVRDTPTPRWMDEDDALLTSLGASMAAMGITSVDLQTQLAAASAFGSFLPQQLQSMQLQRIGSEEYDRMSEPSLYDRNLLFDRVAVHGGPSPIRTQRGTGGTADGSGTADGSITAASSVVAVAASSSCSGGIPASGYPGGGGGGGNCTATGGAMQTQLQVLPPPSPDMRPLRQSTLTEWPSVESSGVALSADSASIAAPPPTPADPPSPPPSSAASGSMATSMSPPPPLTLRPASISSPPGRTLMAAVAATADPPPGPTAAVESPRAFSRPKSPLRLPSPASMALPPTASYGMRRSASAVGALSHLTQLLPEPAAVAAAAVLDADSDLAPPWGLAAAAAAVAFATAAAASTAPSSVHGGSAGGGCGSRTGSVTGMARYRTVFGGGGEEEDEATQAAEAAEAAAIDNDGSGTGEEDLRGDLNAQDDTDEDQVYDGSSVQHPRSPSLMSRPLSPPMPLSPRRHGSFSAHAGVGVGVGSSRSASPALMAGGSSGIGGGGGGAVVGSPSSRSLLSSYGSPRLPSTMLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUK8</accession>
    <name>A0ABQ5RUK8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34131">
        <property type="entry name" value="(RAP ANNOTATION RELEASE2) GALACTOSE-BINDING LIKE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34131:SF3">
        <property type="entry name" value="(RAP ANNOTATION RELEASE2) GALACTOSE-BINDING LIKE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="690"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="772"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="422"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="535"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="704"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="820"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="840"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="977" mass="98101" checksum="53C392205C0DE8FB" modified="2025-10-08" version="1" fragment="single">GMITTRRCIEICYHRQALWTFKPEGKMAHRPVTRSARENIIQITQPKFTTAKCCSRRSVIVHSRQRNFGTESLEHAIQLINKFIFEHEQRRQGRRSRPGEAKGGTEVEVQPVSKPMTFRASAKTEKLVKEPPGPSSFSRSLAAYLALPLDQYSLLDPGWISRSPAAPDVFVLRIPLFDLVGLELQPQITVRVFADLRNGQVHFYADQFKVGDPRFDADFKLAMKATLRNKPVPTIRPLRSIKRMWARAGGAGGQSGGAAGSGSGRGSQAAASAARRGSGDERVSPDPSDDSSEESGGAQVVDAGSSFQGGGASGVSSTLVGIADRINSGPSARGRSDSHAASNGAYEPSGVLDIGLEVSALATSGARPTPVVQPHVNVTREDDEGTAAGIDPPSAAALSAPAASHHAGLGKCQDGTVIEDNTSGTSTSSKSSSSSSGRRSLIWHRTAVSLFDKGFGGGSTYTSTNISTRSLGSASGVAGVETGAVVGGGDEDSGTRGWPAASYGAAHPATTAAPAARAACSGSAMSSTSLTAVQTAAGTTSAPSHPSAAAARPPPSAAGPALRQPVEAAVVAKVAPPPVLAAAAPLAPTSPPNSSSLPFIEDSMLYVPASQWGRMPSGQAGSGASQTGSTITVAAPSSVMASMAKANGTTLQNSSAGGTDEPEFSACLSTAASGIGFIEAGVGRAGRVVSGNTSASGAIDTRSTRATSATPGAAGTTGVVNEAQAWQGGSSTVSAVAATAGVAVIDSTDRNATSSMRPLAFAVAPASVTDTVPHTPSGGGSNPCMGAAGSGPSTQPPWAAAAHDPSGPAGQAVAEKSDSDGSGSRRSSSPGSFTADPRTLTPTLTTNQQDSNSAARLAIASGGGGAAASGADEGATALLVGSVDVAVTVMLPPALSAVPRPLLGMAGSIIARYAISSLLPSFLDLLVADYGRWSSGQTVAARAAPVGNLAAVAAANAAAMGAAAHGARAPQGQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUU4</accession>
    <name>A0ABQ5RUU4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="135" mass="13623" checksum="7FAA42C5F4B99674" modified="2025-10-08" version="1" fragment="single">MQASNLANAVSALAANTFQFVGRIAANASQANRWEWLSRLTQPPNAAVAGGIDLPNQPSPIPRQGGTASPEAPTSPIYAPSPFYVHGPAVVSNHLTPGAVDIPGMAPAAAATTCAAVAGTSSRPPGPPHTGHEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVJ8</accession>
    <name>A0ABQ5RVJ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">aspartate--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Aspartyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_004020</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04320">
        <property type="entry name" value="AspRS_cyto_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="251"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI61602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="547" mass="60622" checksum="919C1FC3A327CB9B" modified="2025-10-08" version="1">MSELGVEGLSIEDSQSSMASTTSAEAKKAAKEAAKAAKEAAKAERAQQRGVKAAVMTQPDPEDPLREQYGDYAMVQSKTQSGRTWDKVESLSKDLSEQKVLVRARIHTTRGKGKSAFLVLRQRTATVQAVLFADDQTVSKGMVKYATQIPKESIVDVEGVVKVPQQPIEGCSQKDVELQVTAIHAVSRAAALPFELVDASRSEEEVRAAAERGEILPTVGQDLRLDNRFLDLRTPANQAIFRVQSAVCQPQLFKDTLLVEGFQEIHTPKLIAGASEGGAAVFRLDYMGTPACLAQSPQLYKQMAICADFDRVFEIGPVFRAELSYTHRHLCEFMGLDFEMAIFEHYFEVLDVIEKLFGAIFEGLSTKYAKELEFIAHQYPFEVPTFKPLRLTFPEGISLLQQGGYPDVDPFGDLNTELERTLGRIVKDKYGTDFFILHRYPSAVRPFYTMPCADDPAYSNSYDVFIRGEEIISGAQRIHDPDLLVERATACGIPLDTIQSYIDSFKLGAPPHGGAGVGLERVAMLYCGLNNIRKTSMFPRDPKRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVQ4</accession>
    <name>A0ABQ5RVQ4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_003891</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="232"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="265"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="410"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="618" mass="66437" checksum="DE515EF5A42DCF77" modified="2025-10-08" version="1">MVTKDADSKGGATNRPVAMTSKTSVSNAAVSRTAASPSRLRAGTTAKPAAIPAATSSASSKPPGVKTSASKSGSSLKFPATASKPAGASSSSKSGSTTGKAGSKGGAKGSAKSGVSKGSAEHEAALEAADPGPDPAEEAARKAELEAKAAAEMEALRRAAWQAQMEYERKQEAKRRQQREEEAKRKKEEAALRKSLLEAAYEGEDDQVNAGLSRAREAGYLRPDPVEAADGHGNTLLSEAAAGGAVRTVQMLLQLGADPNSRGEFLRTPLWRAAFLGKEEVVPALLEAGADPRIGNEGGELPEHVAPPAIKEIIKAWDVAKTDKLIQQIESRRLECVAAVQAERAQAVRGCEESLAAAEEANSRVQQALKHARMELEKRIHEYDTCVEEKKPEGLTQVALDQVHLAEQVVEDTRRRAVEAAEALDEARLRLRREQQQQLAEGGAEAEALPGVQVAVRALNDVLIRDVGGFIAASGKWPLVIDTSGQASVFLRYQDTNYVNALNSRHMEPHMLRRSLLGSIRYGKAMVLDLQDCDLWPEMPRMFDQVQPGLLATLMDKSLLAGERYASLIRAEDGEEYDKNKFQESRIKAFGFLVITAARIPNPQLLAQTYAVRVMITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW80</accession>
    <name>A0ABQ5RW80_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Erythromycin biosynthesis protein CIII-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010610">
        <property type="entry name" value="EryCIII-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050426">
        <property type="entry name" value="Glycosyltransferase_28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050:SF13">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE UGT80A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06722">
        <property type="entry name" value="EryCIII-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Erythromycin biosynthesis protein CIII-like C-terminal" evidence="1">
        <location>
            <begin position="80"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="211" mass="22357" checksum="ABE1A1E203A75467" modified="2025-10-08" version="1" fragment="single">FGSHHHVQSFGFSMHSRLANLARPWTDLWVDRKPIYIGFGSVTLAEGRALALEIREAVAATGVRAVVSAGGWDCLGIAGGNGSSTGSVTSDILVVRDVPHTWLFPRCAAVIHHGGVGTTVAGLLAGCPTFVAPSFGDLFLWGELCGRAGVGPMPVPIYKLTRIDLHYAILVLLSEDARDAAKALGGRLRQTDGLAAAVQHMYRGLTVETSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXK8</accession>
    <name>A0ABQ5RXK8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Letm1 RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_004577</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF1">
        <property type="entry name" value="MITOCHONDRIAL PROTON_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="706"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="10">
        <location>
            <begin position="688"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="341"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="907"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="456"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="745"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="71"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="341"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="929"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI62003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="944" mass="97900" checksum="D59811FFDC83740B" modified="2025-10-08" version="1">MDIRRGQLSRDAVASTSGRHVLQRPSPMGLPSHRLPWVSPATRPNLSQHYVASTHVSVVACKAAPGESSAIPPTTTTTTTTTITTIPTPPLPPNGAAVAATVLLPSQQQAQPPQQPPSAAEEDSRHTSVERVLRLNTLVETRRLYRNIVRAEYLLNLPSPLYTPPPVSAGEHAEWLAASGSSANASGSSSDGLMDLDSDRLSNGSSSLAGSFSSAASVPRILSGPDEDKALKTALDSVAEMALVLEQLKASLRSLPAGFGTAIFPAATASASEETVREVARQNVLGLRERLAGEVPLLPISSDAAIHYIESRNVKFNRRVALDELQGGGWIGISGSVGNGSGSTSGGGGGSTSGGAAAPGGLPGADLTGGGSSLRATPLERLEAAERAAGEIIANRIKPALKRASEQDLLQVVQDTGSYLKGLWVRLNGGSPAPGRWGGWKGVVADKELPRPEGTQQRSELEISRLSLDLEALEKRLQEASKKRENKLRKAGLQGRVQMAIQLKGLDAEVLYLSSLLAVRTLQLEMEYVYGSLEAEALDIFNGLHSGGLLIRDGSTAELALLVAEFALLDEQLTLMAAALEGGSAGGATASDDGAAAAAAMGPALINDEVLSKLAVEIPDMRSRVGVADQVVFGGQGFSLTKARLQVRESLDKVREAITFLTRGFKLLGSDIATGARLFLKAALGNTLKPREVSALRRTARDLLTFIPFTIILIIPLSPLGHVLVFGFIQRYFPSFFPSQFSSRRQEIMVRYEELERQLLEAQAAVEVAEEEVELARAREAVARLMAPEPPAAPVVEAPAGGGAAVAKGDDVMGAAPAASAPVGGILRGVHEAGDGAAAVAAAAAGTAATAPVAVGSGRVAAPPVGSTSALSSVDEATLHEAAKKVRILVEQLDEIRDDVHLVDPLSEATGGEVTGGGGGGRKSKLAHGGSGGRGGGDGGQGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXV7</accession>
    <name>A0ABQ5RXV7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004589</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF33">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="571"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="677"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="720"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="953"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1007"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1160"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1280"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="496"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="601"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="720"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="953"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1025"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1281"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1301"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1337"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1359"/>
            <end position="1374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1374" mass="146197" checksum="B2893A223CF01D16" modified="2025-10-08" version="1">MSGHPQQPEPPKQIASVNIYRQDGGVAEVDQQQDVWAELYGIVHGFAETVQVALQAFTPSDEKAVSAITKKRLALRSIQGLKQSQDGIGALLEHLKALQAASQTWQRSMTYASKSHQELMEQVESTTQELHKTSDRLRGVEAQRLAAVREAELLRARTEQQEDVINEARTSLKQREEDLETLQFSVHELQASSSAALQDLDRLRQAAAEAEGRAQAATAALDEAKGEATKAHSLAERHQQVVAKLTADNLVFLMQLKKAEADLAAANQDRAKLRLAAEQQRGPWFDQVRAGVEERVRQALQHSQELEARFERREAEHTAHQEALREQLTKLEEELHAAQNHAQVLQQRLESTNDSKDRAENRCTAAEITAREVQKGLENLAAENRVLQESIRSMRQECTERWVKEQAAIGQVDGLEQRIEELHQTLSQQTAQLAALQRQLDTQTGINRQLMARKEEVEWQLMAAMAKIDGGTVDQPAPLNLRVSGLLQVGGPNNEHQCNSTGAQNPRSTGLGGLAKTTDAPAEAAECHSAFHAASAGGAAEALRPASVLKDCSKGPYKNLSASCDVPVYSTSQGAGDGDSRSSPFTSKPSRALYGQPQHAGPSVVVNGGPGSKGSSPPLAHNLHEKGSIWRESLRTPEATLLIAHDDLATFTQRAARKDLHAVVTSHPGMLANLYRQQQSQPRQHGLREDTVTSGQSNSGAVLNAPARWASLEAASTVVSSPPSSVALSTASGSPEKAPSLLPSEPSSREPGAPAVTIRTVSNGRRQQPSDVRHVASASAAGGDVGLGHEQIVPNAQGVITVSGEDAVSYTHDGLGRLERRGSLHDGEDVWLANVVSTSSSNDSEFFFSAGVVIQHAAPTDVSHNVHAGAQQSQLHQLQQRLQVQLQGTSAVTVTGSPGRHRPFQDAEPRGSLQAQACARAIPSAAYVLNDEEDIIRARAAPQRNDTGRVVITSGTTMRSVTSTPASAGHDIAPAREALAGAQDGSDRTDLGIGVLNIVKCDAHQESVSRSPMHPALSGKAIQGAPCSPKPASSSSPTEPTLTEASQRMSSEHAPAVCSVDAARPFLRELASMHAGTARIPVCVQAGPPFYATSGPLERNDVAVMEHWMHSTGSRTYTAPVLPLNLKTQELTVLASPARSTTSMRSPRSPVHPAIILVADRGSGESAEGRGSARKTATGPDENSGGLSSRMGAGVQAVQPDIVAGVSTLGTATISDLASGLLQVVASVSSFSESAVAGLAEEGLGATARPRVSFHGLTHDSQAAPGGRSDGSTSAIVGQAMGSSMSSSTGSLVHMSGGSGTQQPQHKPNSVKSVRFSDHESDDQVAQPSRRQRESQESQGIGASAQTPGCSQPHSWAKMSRDPRERSRIRFEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY67</accession>
    <name>A0ABQ5RY67_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47342">
        <property type="entry name" value="PROTEIN PTST, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47342:SF1">
        <property type="entry name" value="PROTEIN PTST, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="1">
        <location>
            <begin position="206"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="288" mass="31706" checksum="96ED905427028EFC" modified="2025-10-08" version="1">MMQACNTRCLQSRVDPQAGYIQRLTGQVRFLQPVCRATPQTLADARSSGVASTSGRSLSVKEKLEVLDKAKERRLEALTQDGLNPYKGGAPGIVDVRLYQSAVESQKRMSAKLAEANAHLNYVERRIEEANKDLQDAAAFLKKVAMEFGTTSRLVESTAAAVRFGVDKEDAVAKLVKLCERLEALEASVQAQRTSFSKRVPKRVPVAWVGVASEVRLMGNFDGWTRGFELSAASIDSDGVIRTFEADVPLLPGRYRVKFQVDGGWRLASDWPTENDELGETNSILVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYL0</accession>
    <name>A0ABQ5RYL0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hydroxyproline O-arabinosyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_004964</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056508">
        <property type="entry name" value="HPAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044845">
        <property type="entry name" value="HPAT/SRGT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31485:SF17">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31485">
        <property type="entry name" value="PEPTIDYL SERINE ALPHA-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23452">
        <property type="entry name" value="HPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxyproline O-arabinosyltransferase-like" evidence="9">
        <location>
            <begin position="290"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="199"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="637" mass="68717" checksum="317443B5ECB5BD0C" modified="2025-10-08" version="1">MMKPGARGAPKRSSYNTFITIVCFSIGLLGGYLILGSPRTADHIVAVKTDAGLSHRGSFAHESTKRVWGTRRELLTPRKATAGGSSSLIGRLGASGGLPLNTALGEKPPAQGEVSKEMAAALKQQQSDTSERVAPAKGDVVKEMATALKQQQSDKSETVVQAKAGDTKEKEALMKAQQSDTSERKTGSAETNAREEQAKTAVTGGAATTAAKTATTTAAANTTTAAASTTTTATPTTAAATVSSTAAAAVSPSPSPSPPPPATSVKGVFPDANTWPITPVKEKMPGNTIHTLFTSNGSPYQNIQARIMVGTYNMVRKMPGGERLVALTRILHRTTPDEVMDEIPTFLAQPLQPECDKWCWFPVADRANAFQQWINAAEKDPSLIKAPWLLLLETDYVWVRPLPDPGDAYDRAIPGWSFGFDYIAPSIPIIMQLLKERCPDCDPKQVPNSGPAPVLARFSDFKAATPIWEELSKWIETHEEAKKMLGWVREMYAWDIGVAANKLNIKNVGPPSSPLISQPPHDRVLGNASMYHYTWGTIYKKPGVEKEIWMFDKRTYTAYEHQLKLPAIPMPPVWTDDIVLQDNLKVTKELHDTVVDMISHMNQAIAELPDLTDKVNKQKEAAAAAAATAAAGATKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ49</accession>
    <name>A0ABQ5RZ49_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Hydroxyisourate hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_005543</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+) = (S)-allantoin + CO2</text>
            <dbReference type="Rhea" id="RHEA:26301"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15678"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="4.1.1.97"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23736"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="3.5.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; urate degradation; (S)-allantoin from urate: step 3/3.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05822">
        <property type="entry name" value="TLP_HIUase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3330.10">
        <property type="entry name" value="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.180">
        <property type="entry name" value="Transthyretin/hydroxyisourate hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014306">
        <property type="entry name" value="Hydroxyisourate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018020">
        <property type="entry name" value="OHCU_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036778">
        <property type="entry name" value="OHCU_decarboxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023418">
        <property type="entry name" value="Thyroxine_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023416">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036817">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023419">
        <property type="entry name" value="Transthyretin_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02962">
        <property type="entry name" value="hdxy_isourate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43466">
        <property type="entry name" value="2-OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43466:SF1">
        <property type="entry name" value="2-OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09349">
        <property type="entry name" value="OHCU_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00576">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49472">
        <property type="entry name" value="Transthyretin (synonym: prealbumin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158694">
        <property type="entry name" value="UraD-Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00768">
        <property type="entry name" value="TRANSTHYRETIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00769">
        <property type="entry name" value="TRANSTHYRETIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase" evidence="9">
        <location>
            <begin position="12"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Transthyretin/hydroxyisourate hydrolase" evidence="8">
        <location>
            <begin position="196"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="34955" checksum="150D2A4E29EED316" modified="2025-10-08" version="1">MALFITYDQALSCCASTRFAQQLSAEGPYADLDALLTAARRIWWTQTGVPGWLEALAVHPKIGDKKGMEGKPEAFATFSRNEQAASSQSLNTAVSLELADWNQRYLDKFGFIFIIFAKGKGAPQILEALKKRYERLPHEELQAAAQQQMKITELRLCGLYGVSDSTDPVATRTQRRAEQVLTHLAPAPGGPLRSPITTHVLDTASGLPARGLPIALLKQDEVSKAWETVGEGVTNDDGRVGTLLPPGNYITPGRYRMFFDTSTYLAACKLAHPAFYSEVPFYPEVVVEFIITPDKATEHYHIPLLLSPYGFSTYRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ71</accession>
    <name>A0ABQ5RZ71_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydroxyproline-rich glycoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">HPRG1</name>
        <name evidence="3" type="ORF">VaNZ11_005773</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="629"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="639" mass="67855" checksum="54DE6000029DEBC5" modified="2025-10-08" version="1">MRPSRFVAQAMRFDTAGITATQLQAGEAPRRNASKRASKHIRPVAAILAFLVLAAPLATHAELVATITFYSVNGGFDNGDCDLLDSYLTGILIDKNVPINFVDPSAYRTNCTFTNYSGRVKSVLRYYPIFDSLNALENLIQFATALRDELLWRALFQLVLAGCSAEAFYTDPAVVTGLTANITVCTNNPTSTVLPPNAGALCTYFLSQQLCSPPPPPPVPSLPPGASPPIAPPSPPSPPLPSPPLPPSPPSPPRPPRPPFPPGAGPCIIITKAVRPANWTVTTSCNQLVNSMYSFYSFNAQLVPDRGFRCIDDGSTNGQMIVVGTAATPQDATLISDNFRRRQVIGATILALGGMKCNSAFQLEGTTCNIVVRYDSWMNPELFGCYPSPPPLPNPPNPPPLPPPSPSPPGPNPPPPPPRPPPPSPPPPSPIPPSPPPPSPPPPPRPSPPPPPSPSPPPEPTFLRIKITNPEFNAIYRMNCTALRAAVEVSIQATGQIILNDVSCNVSYLTGETLIRVQMMDPIAAAQAGYALQGFLTTIVTIGRLTCNSLIEESVSTSEFYLSMSCYGMPVLCCPKPPQPPPQPPPPPSPRPPRPPPRPPSPIPPLTPSAFSNSPPPPPPAKPPPPPPAKRRKPKAGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ99</accession>
    <name>A0ABQ5RZ99_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="1">
        <location>
            <begin position="6"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="142" mass="16436" checksum="03D48A653B41666C" modified="2025-10-08" version="1">MAKALYDQWITARENFTFDELTATLLARFTPEAQSRDIEARCMLAFGSFFMFPNETVTAYQSRFEVLVISIPDFSEMDCIYWFQQGLSESVTRQCVTDLIGTRVQSYVELMPFVLRAEMRILARQNALRLVPHVTAMQTQDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0A0</accession>
    <name>A0ABQ5S0A0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannose-P-dolichol utilization defect 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_005988</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MPDU1 (TC 2.A.43.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016817">
        <property type="entry name" value="MannP-dilichol_defect-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 LEC35 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226:SF2">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="201"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="257"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="218"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="259"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI63086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="29797" checksum="E15AD641E928327F" modified="2025-10-08" version="1" fragment="single">SVPQIAVILRTGSAEGLSLTSNLMELLCFTVTVAYNIQQGYAFNTYGEVFACWIQDAFIVGLIFRHMRLSWSIIAASTAAFAASCFWLFSPMCPIHLLSALQMSTVVVMALGARVPQIWLNVKRGNAGVLSPLTCLLNVAGCVVRVFTTAVLTRDGIIMGGCITQLILNAILLYQSIATPGKIRAVGTATATTAATATALAVTDPPSGGAGTTRRQLGGSSQGAAASTAADGNSAATGNSGNIAVSAASAVVAPAVSPGQGGSKLGEAGGSGGSGDSGGSGVSSSGGGGGALPMPVRAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0V7</accession>
    <name>A0ABQ5S0V7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_006497</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051292">
        <property type="entry name" value="Xyl/GlcA_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270:SF52">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE PROTEIN GNT13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046929177" evidence="8">
        <location>
            <begin position="27"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI63512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="500" mass="56823" checksum="F8F91B73BD6906A8" modified="2025-10-08" version="1">MSMVSHSRVVALVFMLEAWIICAVGADEANMLRSCWNAKYNPTPIMFEEWISGHPARLYMVDAWWSSRRNSIPLTIFTMMSLDRLSMLESQCRGYIGPISAAVYIPLVLERDPSLGSDFKATSDHDDGVERHGYGRDDENDDNYGDGDSDGGEGDNEVANVLVGRRRHIQGGYNESHTDPVALARAKLQNLFHLMEAEAARAAGNNSLPGCCCQLRLMLYAERMSDSPLATIMPTNSMRNAAMLGATTPLSAMFDADLAPCTNINDLVQNASWVQYISKETTRPSICIPPAWETRGHLQPNSKNVVVQRAITGIKADVLDMWARSELQVFQVNVCKHCHKTFDHSRWAKEDMPYTVQHTLGFEPWGILWRLRDPGYDERFRGWLYDKQTHVEALARKHRYTFTVLPDLWVVHRPHKKVGIVQLYRRSNTTGEGSREVEALLRPVVLSNGKNTTVYTAYRHYMDSMRRTLRSALASRQPYEPQQNAQFLHCKSVLPWWQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S121</accession>
    <name>A0ABQ5S121_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006014</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="7">
        <location>
            <begin position="515"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="460"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="716"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="474"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="490"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="738"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="783"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="808"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="818" mass="91816" checksum="5FE8C188B3438593" modified="2025-10-08" version="1">MQATRASTADRSRQGGRAQDRDREHDRDREHYRDREHDRDRQHDRDRDRDRHRGRDHDWDRDRDRGKERIRDRDRDRGRFRDRGLDSERYQQVDREQGRERELARRDADDSPRKRRRQDDGPVQKRPEEGTAGGKGHGDAEAAAADAKMPAVNGADGVAASGPTEEPVPRKAVTLGKTGGVYIPPFKLARMMADQGAQDKSSPEYQRLTWEALKKSLNGIINKINVVNVKNILPEVFRENLIRGRGLFCRSLMKSQAASPSFTPVYAALVAVINTKFPEIGELLLSRLILQFKRSFKRNDKPVCTAASKFIAHLVNQGVAHEVLALEVLILLLEVPSEDSVEMAVDFVREVGAHLQDVAPQGLHGVFERFRAILHEGAISKRVQFIIEGMFALRKAGFEASGYPALKPELDLVEAEDQITHELGLDDQLQAQTNLDLFKVDSNYVEEERKYAAISREILGEEEEEGGAGGSEPAADEEGTDGDDEDDGELGSGGGDGGDGGGGGIHDETATNLVNLRRTIYLTLMSSFDFEEAGHKLLKIGIQPGQEIELVTMIIECCSQERTFKRFYGLLAQRFCYLNRSYAETFEDCFRKQYSVIHRLETNKLRNVSCLFAHLLATDALPWSALTAVHLTEEDTTSSSRIFIKYLFQELSSTMGLVKLNQRLNDPAFIPWFEGLFPRDSLQHMRFSINFFVQIGLGGVTDVMREHYKAAFAAQTAAKQQEVTRRMLGNDDSDSESSDSSSSESSSGSSSSSDSDSSSDSSSSDSDSDRYDSRGRQRRRGHSGRDGGKEQDGRKHREREKGGENPRGRKSKDSRRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1N5</accession>
    <name>A0ABQ5S1N5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23011:SF28">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="96"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="204"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="593"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="839"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="903"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1097"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1261"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1448"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1538"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1596"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="839"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="909"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1097"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1330"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1395"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1538"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1567"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1596"/>
            <end position="1612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1637" mass="167003" checksum="F58B5FE6E7199721" modified="2025-10-08" version="1">MPTTKEAAAAAAATAANDALVGQAAVAAQQAIFARQAAAAAAQRAEFTYLTGLPPNRRTVAQNTRLAVFLGNAPRTQQAGPTALHALSVAVQVIEVPRGGTVYRINDQSDGFYVLVDGWVLLYDVKDKDEAEEDDGVVDRLGRLDSFGEEDLLSSSRRAHRADVCADSSAVLIRVPPELYRKYLQHLHQPDFEDKVEFLGQLEVFKSLPDETLRKLAPCFAQVELHAREYLVRQGEHAESMYAIASGQCSVLVDPGFRAEQGAEPEVDPKKAMQVCTIGPGNIVGDMTVLANVRRRTATILCLTDLVCYKIRRATFIRRVPSMQLEGLRQVAEAKLRITERHLHISVSGPGSLAPGSKALTLRDKLLRGHDLRQMLPISNVPPTELRGLAPRIQNETVADFITNSLAAAAAAVAAASNGGGCSGFAAAGSNSGATSGGNTLGAASSVASVAPSSAAVTAAAAVAASTAEARLRFGWHQKSTGLSPRGRALAQMTVATSHGVGGTGSGGGVGVALTDRTSAQAAKLQGRNSVATCASTTADKVRHADINFSWGLGPGTLINSLLDRAPTSGGITLHRVYATDTEAAAPQQALGNESVNSKSDGGSGCPPVPLVPLLPTQPLPSASAAQLRPTSSRQHVHHLSFNYGRSGGSGGPGLRGSASAVALLQPRWGWTSSPIDASYAPYMAPSSFGTTPGGTAFSVVGISVNVTGGSDGHNVLVNGGGNANGNVSVGSAIVGPKLRRGGVRSAHNHSSATLPYINGDKTQVVQDPSHANRRSFSGGYVAAGGSGSTGVAQYAGLQRPGPLVIGAAAPAGILPPPQSLPALNPSLVQLLQQGAQSQLLQSASSPQAWPAQQSTPPQPQQLQQQQRCSGSEGGSTPGVGFEKRSAIEATVEDPGSGIIASTARSQESPSSPFNNPSSPARSRHGDDFLHQDQRCASGHVTRGTSVKIRRGGSAAVGIGDEDVSSDIEALTPSRRDGARRTRSMCKARFSRQMVTQNSSVDALVVEAIINALAKDIMLATQATEAEGATSAEAEETALNGGALAVDWPLGGGTVPGQGTDGVEGPEAETGMGIGLGGEVGEGLQEPEYTPDAGVDVGGAGDCEGAGEGGVADQGGDVSEGAENSSPVEGTEERVGSAKGSNTVGVAKAGGDGVSADEEEGQAAQAMEDTTAVQRDEIPEDVVEAEGDDQACHTVCEAEETNTRGAELQPVADTPAADGADAAALQEASLPEDAVEDALAVASIDTPEPEKPATPIAKEWATAEHPEVDSQTEVDAITLANGQAVPGTPSSPAQGDANDAGSGTDASPFPNSQKRESWAMSEGSTVSMDSDSHQHKERPEQQQREESGVLQQSSMGAAHDQISSDLEGFSGPMSGVFAKATANASVAESDKQEPDPQLASQDAQQPAPQAHSQAQGSTSGVIYPGLVSRPPTTLQLTLPDAVAAALHARPGSSLSPNRRQMTPPVAPRPYPYVGPFLSFKKSPQLPLNLIAPPFPSAAIQHSKAPSFTLRGSSTASHSNPANFASPAPVHAAVPAPAATVASSGTPPAAGPATPTAGVPAAAYGERQGVGGSRPSGHGRGVVDGLGSISAVVGGGGNGGGAGGFHAGGGSSGTRGQLRKGGALYRPLTGPRNSSVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1V6</accession>
    <name>A0ABQ5S1V6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S49 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006143</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S49 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07018">
        <property type="entry name" value="S49_SppA_67K_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004635">
        <property type="entry name" value="Pept_S49_SppA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047217">
        <property type="entry name" value="S49_SppA_67K_type_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047272">
        <property type="entry name" value="S49_SppA_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00706">
        <property type="entry name" value="SppA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33209:SF1">
        <property type="entry name" value="PEPTIDASE S49 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33209">
        <property type="entry name" value="PROTEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S49" evidence="6">
        <location>
            <begin position="224"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S49" evidence="6">
        <location>
            <begin position="486"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="796" mass="84552" checksum="C0D5999972D4B80A" modified="2025-10-08" version="1">MSMNVNCRPGAPVGVAISTYRLFPATRCQAGGLLLPRHPAVSNLPQHLRSPCYHRALHRGDALRCHAEQQSDGTSSSIVTVSQQEAAAANSSGPTDPQLDDTATAPAVPPARGVAAFPAVVLEGEELPFNAPTERDRFWTNLKLAFALPWRRFKKDSVLTFTLEGEVSDQSRSFFDSGLSIPAVCGALQKAALDPRIQGVAIEIGPLGIGWGKVQEIKRHIDYFRSSGKYVVAWMKQGAEKEYYLATACSEIYAPPTAYLSLRGFVTGGAFLRGVLDKVGVEPQVKRIGAYKSAGDQLLRRDMAPEQREQLSAIQEDVVEEFLRQVAAARGKTREEVVAFMEEGVYDMDKFKSGGWVTDLWYEDQLLEELKMRTTGLRPREGESEKEKEKREKTLAAPLRRVGLRKYASVSPSAFGLAGGKKRIVVLRTAGAIVGKSTGAAITPDGVIPKLRALAKDKSVAAVVLRVDSPGGDALSADLIWREIRQLDAVKPVIASMADVAASGGYYMAMAARAIVAEPLTITGSIGVVTGKFNLAELYSKIGYHKELISWGRFAKLLADNQGFSEEEEELFDASARHAYASFRNKAAASRGMEVEDMQRVAQGRVWSGGAARKVQLVDALGGIHRAVSLARHAAGIPADEKVTVLELGKVRPSPTVLLGGGAMVGAVLLGLLRGQSPGQALAVATGAATLASTAATAVEGSMANTAAGAAATALPALGLVAQQALTMAAVLQPGRCAYLLTEVDPLSVGCSSGALMGGAMSGGRAVTADSDDENGFLSEGLQDAGLVLEQWLRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2S4</accession>
    <name>A0ABQ5S2S4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="24"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="153" mass="17583" checksum="80E5472EE6A499D0" modified="2025-10-08" version="1">MNIAQKAYNCLYKSLCMLRWMGIQGSWMVKVDLVDAFYHIPICAANHYFFVFRFCGVLYQVNALPMGWLSSPYWFSNIMHNMMHFWCNPLAAVGGRCRRLAPPLLPHRFYPPARCGRPVQLGLRVLPYLDDFFVCVCFGGAGPCRGPVGQREH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2X5</accession>
    <name>A0ABQ5S2X5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_006935</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07835">
        <property type="entry name" value="STKc_CDK1_CdkB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF548">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE A-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="57"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI63833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="365" mass="41540" checksum="012826B5D517DF94" modified="2025-10-08" version="1" fragment="single">SPSYKRLLEVCYLLIRFLLLYRYLGFCRTAFGKLAATVRGPLVGLSSTGPDLVMDKYERLERAGEGTYGVVFKARDRYTNEIVALKKIRLEQEDEGVPSTAIREISFLKELRHDNVVRLYDVLYSDRRLYLVFEYLDLDLKKEMDSQRVKGNMGLIKVYMWQMLSGLAFCHSRRILHRDLKPQNLLIDRSRNQLKLADFGLARAFGIPVRAYTHEVVTLWYRAPEILLGSKTYSTPVDIWSVGCIFAEMVNNKPLFPGDSEIDQLYKIFQLLGTPDETVWSGCSALPDYKDTFPKWRPQNLAAAVPALGPHGVDLLSRMLVYTPQHRITASAALDHPFFDDLKSQMRSSGAQQAQHGGGLLAVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2Y3</accession>
    <name>A0ABQ5S2Y3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39444">
        <property type="entry name" value="SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39444:SF3">
        <property type="entry name" value="SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="313" mass="35304" checksum="9D70689B280D269F" modified="2025-10-08" version="1" fragment="single">MNDLDKDPVVDFTGHFLKRSSLKGHKENQGVSREIKVRGQLAEQRCIAEGKGRSSGSESKRKSEVPNPKEHFTHLENSSASHKKRKTVSEDETGGRTKAKCASKGLWPFRVDYNDHFESPLQAVQDIEPVLQLLSVKLKKSKSSLTVYDPYFCQGGIRQHYEQLGFKNFIHRNRDFYKDVDSGNVPSYDILVTNPPYSSDHKERILEFCLRSGKPWALLLPNYVATKAYFSELLDSMSTPAAQRPFFLTPPARYNYEHPEGTGHAESPFFSIWYIGLGEHTETIYKACKARLEPQSNDSSIQSAAAAGLGGVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3F7</accession>
    <name>A0ABQ5S3F7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Programmed cell death protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13788" checksum="518BD0C5019D53BA" modified="2025-10-08" version="1">MQAEEQQKLAAMMAAQGGQGAPSQEDLEAQEEAKRSAEEQRRTMLAACMTNEARERLARIAIVKPEKARGVENMILAAAQRGALSAKVSEERLVQLLEQVNEREGASKPKITIQRRRPNLFDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3Q0</accession>
    <name>A0ABQ5S3Q0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007776</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07833">
        <property type="entry name" value="STKc_CDKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF111">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="671" mass="71227" checksum="199FD232775D7F73" modified="2026-01-28" version="1">MNKYEIISIVGEGAYGVVLKCRNKETGEIVAVKKFKESDEDEIVRKTTLREVKMLRALRQENIVNLKEAFRRKQKLYLVFEYVEHNLLEVLEEHPGGLEHEQVRNYVYQLVKAVGWCHQHNIVHRDIKPENLLISPSSPGGVGKLKLCDFGFARQLPPPDVSITDYVSTRWYRAPELLLGSTHYGKEVDLWAIGCIMAELLDGQPLFPGESDIDQLYILQRLLGPLTREQHELFLRNPRFNGLKFPDMRNPETLERKYAGKLPHDALSFMKGLMAVDPAQRLTCSQALAHPYLAALEEKQSGANPSANRVTSGGAAETSSARVAGRKASLQMGSGGGAAGDPMDEDMPSPPATSRHENMDHDMSDNESTASTIAVARRKAAAAAAAKGGSGGSGHDGRGNASFRGSGRRDINEMHAAASATLGVMAATGAPEMYGGRLDSAGSRAGTPQGGPGSSTGGKSVGYAGPGAHPQPRQSHLGVAGERFSASSRSTQQGGMPKQSPGRHNNSPPHSHLDSGSTYGGSTAGRPSMGTNQPVLYQTNAAAGASKLSRAPSRGDPWPGGPAGGQPGRGNIPPLAPGGGPRVSGHWGDDDVLSVSNMHLRTSADDVASSGYGGYGNGGRAAPRPAPASDPNPERPYSRGMLGGGGAAGAYGGNQMWPQLSMQQQRNRGNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3Y7</accession>
    <name>A0ABQ5S3Y7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="264" mass="28041" checksum="2654B92A33E7A383" modified="2025-10-08" version="1" fragment="single">MAGPNSLSIFTLRVPLLLPKLLVMVAMVLSVVPLVAKCHSSRLSSRRNLQNTDNAWTTPSQQPATQSLLFGNRSRAWYAVGLLPGPLDLAADSFSNTYDGTGQPRNCDERTILAASVQMFGLPQATQSFTLVAILNPPLREPGALQGDLMTAGGLRVQWDLSANVIRIFASWDASASVVAVLPSGANKSASWPFITVSLSRRADGALGICFNGAPMTLQQVRPTTSSQAPGGWLLNTPLGSGWSLVSTDRLQLGDPRAATTLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4I1</accession>
    <name>A0ABQ5S4I1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008201</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="39"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="317" mass="33822" checksum="443B157126F94371" modified="2025-10-08" version="1">MQTVLAKSSFMGARPFGVAGKAKWTMDKKLPFSLPSFGSEKKSGTVAAKPKAGTATTKPAPKATIKKVASPTGTRSGGVGYRKYQGDALWLPNTVRPEWLDGSLPGDRGFDPLGLAKPSEFVVIGIDENDQNAAQNKKGTVEAVVAASPDEVSSENRLAPYSEVFGLARFRECELIHGRWAMLACLGCLVAEATTGVSWVDAGKVELDGASYAGLSLPFSITQLIWIEVILVGGAEIYRNTETNPEKRCYPGGFFDPLKLASEDEDRAFRLKTAEIKHARLAMISFFGYSVQAFTTGEGALGSLAKFADGLNTGKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4L0</accession>
    <name>A0ABQ5S4L0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="363"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="616" mass="62091" checksum="5BA8431A09751204" modified="2025-10-08" version="1" fragment="single">MSSGGSDGDSVTSSSLLGKGTSAMPKLPPRPSATKERSHIAECTCGESERSADGTSNGGESVTAPVTAGAARVAATEGPAPGSSNVIISASSGPSSRPTSGPSLPPRTAVARSKTNATVTTSNSSTGTSVNFTNDSPALTAASVSVAPAASMTKSPVPWGTSGVDNGSSSGGGAGGSYTTKAESPPVTSGKMGSASPTGSGGGAAPAGLRGLRKAPMGGLPGLPPRPAPATGPVLDPDEPESLAEVRRSIHAVRVQMISAALRLGYDHGNALVKQVLYRLLLAERLKAPWNRPGRRPDPVAAAAREAARREEEALKGMEPRPSSSQGGAASTSAATSSSSSAYTGPGGGSGAPELPPLGFTVKIMLIGLQGSGKTQLAYALLGPMGRPHTAVHPFTGSTNRVTVMRGSAHGIGLEFIDTPGLSLAPGGAARNCQVLQQIRRAYQAHRPDLLVYVDRLDAPTPAAGPATGPGAASIGGAGAELAVLRNLTIALGPAMWLNTIVAFTHAGAPPPSSSRAPGTHRSGTNPAPAHTGPSFENWLEIRSHALQGVIRQASYDERLMNPVAFAESHPSCPTNTAGQPVIYNGMPWRSHLLLMLTSAKLLADTEALLQMQGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6H3</accession>
    <name>A0ABQ5S6H3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="842"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1024" mass="104267" checksum="2CB83D1649B523A5" modified="2025-10-08" version="1">MVTNLAAAHDLLKSIAAANAAGDPDATADGTALLLRLIQESEIEEADAAEQVLCFFLEAASPATPEGESIRSLLGGCVLEVLPRLMQLATVSPQSATAGAALVELLANGMPGLPTAGPYCREVCSPRELLTALLEVYDLHSDADPDAPTHMMLLRLLDLIPRLVAAVQRRRLPVALSCLAAVANLAATEGQKLTVVREMAEAGAAAVATTAAVAGGRRAAATARNGGRSRVSGDGHQAEVEAEAEAETIMEIWHEEEMRLVAPFVSFAQQLQDLVLETELSALPLTSRHTTADSNAAARGAAASSCERRRLSAWLLALFGALSPAWGPIPSAGGGGGGGGVAAPAGSWCGTQAAAALLNLLLGTYLLPPPSQSGQQQQQQQQQQQRRPGISVENVGGSRGVEHAASSGNGRGGGGDGGAVDWSSLWAAAVAAADSDMEVAFDPELPLLGAATILWLATAASAPTAIATPTASTAFATATTASATAATATAASARGWNCPAAPEAREGDPLGALKVLLEACDVLLRYAVAWESAVQLNRALQLLAAGSARVRRMAAVGAAKVPVVAPAATATVIASAATATAAIADAAATTPHVHTVELSGTIGGELTAIWASHPLPPLPSPLPLGIQTNDPWVTVANQLASRSGLVSANELIDVLDEYTPRLGEREQVLRPLADRLASTAAINVAHGSPGPGADATRTLAHTALQLLLDALSPSMRALCLAYLAAGPSPTVSALALQRLQREIARTLAVGHEPAPIAGGTRGSAGGGDAGNYAAFTGPWVLLLVLEQLLQLVQQVNEAEPSEAAVTTLTHHADAATAAVSVLRFLGLRQRAAEAALAEAATPKELTNSGTCTAQREPSREATDESGESTTPILAGNGTVAPQLNVVVAARGAVLAAVYNAAVRPLSGTIPAALSILQRQVELEEGAVGMAASPMPMPLPSPSPTPPPSSSLADGAFKPMTALPHTLPLTQQQQQQQQGVLGGRGADRFAGNVAVVSDVVAGLTALSRLQEVIDYVVELLGHNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6M6</accession>
    <name>A0ABQ5S6M6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_009041</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TPPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="EF_hand_dom"/>
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        <property type="entry name" value="TPP/p25"/>
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        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF66">
        <property type="entry name" value="OS04G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05517">
        <property type="entry name" value="p25-alpha"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="71"/>
            <end position="106"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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    </feature>
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            <begin position="319"/>
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            <begin position="370"/>
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            <begin position="778"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
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            <begin position="1148"/>
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            <begin position="1226"/>
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            <dbReference type="ARBA" id="ARBA00010994"/>
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            <dbReference type="SAM" id="Coils"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
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            <dbReference type="EMBL" id="GLI65565.1"/>
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    <sequence length="3870" mass="379090" checksum="F34800B1B57A27A5" modified="2025-10-08" version="1" fragment="single">MVGNREASRRKIGVADDADGPAQVAEPEPGPKKIGESLKGPDPGGLEPPGRSISPRTVPARQEASPERALPPSVRYADMFRMYDTDNDNSIDHTSMVAAFGQLGLLNGLNPKLLAQVLNLRDSEAAIRARCYPPHEFISHCERLSYWQARQAREHRIKVASKVPGIPRGAERAELLRRVYHRYAVSPGQGRLVSDADYNEPRMTSIQLQKVVSDMGLMQPNGPLSIVSVDLVFTKCKAPGTRKMGYPQFLRALAALAEESGADVWSAANTLGIKLRPSGPPPMDAVFGAGYRYEDDPGTYFADQELPVDEFGTVQQGLPKLPAQQGTSLPDRPPITKPRSKYFSQKNLDLTGAAPSEGVTTSLPNGLGLASLASKGRGPGGGMGSPGPLGNGLSPLPRQAFGNATDNGESLIKEPLLGGGTMSAGRTLSGIASLPQGQAVLAAFKNDGSSDPLAGKPIRAFETPPSGEGDIVGDGVAAQLQKRLEAMERQMRAQAAALEEARATAAAAQLAAAAAATGGKGDVASLGELGRGSATSILERLAACEAGLAKEKAAGDRREKELAAAAAAAAQRDKELDDLKAAAAQRDQQLAEQRKQMEQLKALGTLAGVAASAGKDGKDGKDGLAGEKGDGVDLDRLTALERRVAALEGKLKSVDEDAAAVRTAAEAAAQRAEELEAEVAAAAAAAAAAAAAAAAAVTSTSKAETAGEGDSTSSSGGDVNGAQRVSSTGRNPAGDVSGAMTTGASSGSATAADDVARLAASLPAALAELDRRIKELQQNRPAETAAATTASDKKGGKVAAPSSSTAIAANSVAAAAAELAAATGAITSRNRQLGDQVKALEEQLKQLLANVGKLAEQQQPMEARDGAAGAMGAAGAVGATGAVGADGVNGAAGIAAGGRSAPTEQQQKSAVAAGGPGKAPAASKAARDALLAGIRGDINAIQMALGEILKNAARRGRGGAAGAGAGASDADLAALAAAAAEDREKLRKLQDLVAALQKELAGSGARANEAFDAVKAAKDDAAVARAVAADAAERAEQGQLEAAAAAAAASAAAAAAAAAIASADGSGDVSAAGMGLMAITDPAGASEVSVGSRAAGFGEGGKVVSGESGAGLPAGKGAGSSAATDDIARLAASLPAALAELDRRIKELQQNRPADEGPATTATDKKGGKVAAPSSSTAKAANSVAAAAAELASATGAITARNRQLGDQVKALEEQLKQLLANVGKLSEQQGDSKDEGTASASDVHKAPAGGATANSISSDQQKKGVATAAAGAASKTANPAGKAARDALLAGIRGDINAIQMALGEILKNAARRGRGGAAGAGAGASDADLAALAAAAAEDREKVKKLQDIVAALQKEQKGAKDDAAAARAAAEAAVERAEQLEVEVAAAAAAASAAAAAAAAAVTSTDDGDMAMRQLTSPTGTAADPAWASGAPTGGRTAWSDATIGSKTSAGPLPGDSSSVAMDDVSRLAASLPAALAELDRRIKELQQNRPAETAAATTASDQKGGKVAAPSSSTAIAANSVAAAAAELASATGAITSRNRQLGDQVKALEEQLKQLLANVGKLAEQKGSGQDAGVPTAHGGAAGGSSNTSGTATSNASGHDAQKKPIIGKAARDALLAGIRGDINAIQMALGEILKNAARRGRGGAAGAGAVASDAALAALAAAAAEDRERLRQLEALMAALRTGHKATQDDAAAARAAAEAAADRAEQLEVEVAAAAAAASAAAAAAAAAVTSADDSGDVSAAAAEQLSSPTQPAADTVQSSKAVGQSSTAVPAGRTDGSRKDAAVSSGTSKPGVLPGSDVTDDVARLAASLPAALAELDRRIKELQRNRPADEGPATTATDKKGGKVAAPSSSTAKAANSVAAAAAELASATGAITARNRQLGDQVKALEEQLKQLLANVGKLSEQQGDSKDEGTASASDVHKAPAGGATANSISSDQQKKGVATAAAGAASKTANPAGKAARDALLAGIRGDINAIQMALGEILKNAARRGRGGAAGAGAGASDADLAALAAAAAEDREKVKKLQDIVAALQKEQKGAKDDAAAARAAAEAAVERAEQLEVEVAAAAAAASAAAAAAAAAVTSTDDGDMAMRQLTSPTGTAADPAWASGAPTGGRTAWSDATIGSKTSAGPLPGDSSSVAMDDVSRLAASLPAALAELDRRIKELQQNRPAETAAATTASDQKGGKVAAPSSSTAIAANSVAAAAAELASATGAITSRNRQLGDQVKALEEQLKQLLANVGKLAEQKGSGQDAGVPTAHGGAAGGSSNTSGTATSNASGHDAQKKPIIGKAARDALLAGIRGDINAIQMALGEILKNAARRGRGGAAGAGAVASDAALAALAAAAAEDRERLRQLEALMAALRTGHKATQDDAAAARAAAEAAADRAEQLEVEVAAAAAAASAAAAAAAAAVTSADDSGDVSAAAAEQLSSPTQPAADTVQSSKAVGQSSTAVPAGRTDGSRKDAAVSSGTSKPGVLPGSDVTDDVARLAASLPAALAELDRRIKELQRNRPADEGPATTATDKKGGKVAAPSSSTAKAANSVAAAAAELASATGAITARNRQLGDQVKALEEQLKQLLANVGKLSEQQGDSKDEGTASASDVHKAPAGGATANSISSDQQKKGVATAAAGAASKTANPAGKAARDALLAGIRGDINAIQMALGEILKNAARRGRGGAAGAGAGASDADLAALAAAAAEDREKVKKLQDIVAALQKEQKGAKDDAAAARAAAEAAVERAEQLEVEVAAAAAAASAAAAAAAAAVTSTDDGDMAMRQLTSPTGTAADPAWASGAPTGGRTAWSDATIGSKTSAGQLPGDSSSVAMDDVSRLAASLPAALAELDRRIKELQQNRPAETAAATTASDQKGGKVAAPSSSTAIAANSVAAAAAELASATGAITSRNRQLGDQVKALEEQLKQLLANVGKLAEQKGSGQDAGVPTAHGGAAGGSSNTSGTATSNASGHDAQKKPIIGKAARDALLAGIRGDINAIQMALGEILKNAARRGRGGAAGAGAVASDAALAALAAAAAEDRERLRQLEALMAALRTGHKATQDDAAAARAAAEAAADRAEQLEVEVAAAAAAASAAAAAAAAAVTSADDSGDVSAAAAEQLSSPTQPAADTVQSSKAVGQSSTAVPAGRTDGSRKDAAVSSGTSKPGVLPGSDVTDDVARLAASLPAALAELDRRIKELQQNRPADEGPATTATDKKGGKVAAPSSSTAKAANSVAAAAAELAAATGAITARNRQLGDQVKALEEQLKQLLANVGKLAEQQGSGQDAGVPAHGGVAGGSSSTGTAGDDGAQKKTAAVGKAARDALLAGIRGDINAIQMALGEILKNAARRGRGGAADAGAGASDADLAALAAAAAEDRERLQQLEALMAALKKELAESGARLDEAKDDVAAARAAAEVAAERAEQLEVEVAAAAAAASAAAAAAAAAVASSVEDGVESPASAAPAAAGQGSLPSRPSADGGRSSNPKGGSEKTGSVGKKAAGSGSSLGSTQVAGTSKGGQSGAPGAAVVATGAASSAADRSSAADDVSRLAASLPAALAELDRRIKELQQNRPAETVAATTASDKKGGKVAAPSSSTAKAANSVAAAAAELAAATGAITSRNRQLGDQVKALEEQLKQLLANVGKLAEQQGESMDTVTGFGESTGANASVPVTQRTTGAPGAGRGSKGAPIGGTRGTTGASGANEATGTGGIKSSIDQKKTGDAITGSGKAARDALLAGIRGDINAIQMALGEILKNAARRGRGGAASAGAGASDADLAALAAAAAEDRERLRELEDLVAALKKELEGNGARVDEALDAVKDAKDDAMAAQATAAAALAAIAAAVSKGEEDEIAADAKVGSAPGGKDGKDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6Q3</accession>
    <name>A0ABQ5S6Q3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_009171</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF29">
        <property type="entry name" value="AFG1-LIKE ATPASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="93"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="598" mass="63411" checksum="9C90EFABE2B20C5F" modified="2025-10-08" version="1" fragment="single">MRVASSLLRYCRNPSHILPNAGNTGAHLYAFSTRAHDAPGGDATDNALSNDLLSTTYCQLLGRGVLLPDATQVAAVHALQLLQNAVLQYEKLRSSSSGEGNARGEFRPRPEGGVSDSSSSSSSSSSSGVVVSSDSNNRQVGDIKIPATAGGGEIPVVQIAPKPRTIRGAYLWGPVGSGKTAMMDIFASTTQRCLEAAAAATAVHPPHLDSAVTPAAAAAAAAAGTAAPHLLLRSCERHHFHEFMQSVHGRLHELHKALPRVVARSREGRMVYRYAEPDEDPLVTVARELGSRTAVLCLDELHVTDVADAMILARLFAVLLVDFDTKVVFTSNRPAKDLYKGGLSRKYFEPFVQLVDEQMVVLRVAAGVDYRKMAGVTVKAAKAETRKVEAGVVRVGNGCSTTPDAEREVDESALRKGRGDYWYFGFGASKRLHSQWRQRAAAAAAAQSARKTDGAASGDGGGSGPLDPQLGDVGVPVTVPLAYGRTLKVPCASGDAALFTFEQLCGANGLRAAVDQGGALAAPDFLALCRRFREIFITGVPQLGPADRDEARRFVTLLDVAYDNRCQLSVAAAVPPDTLFAPLLDEARRQGVNPRLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6U3</accession>
    <name>A0ABQ5S6U3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008712</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051412">
        <property type="entry name" value="Formin_Homology_Diaphanous_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691:SF6">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045872035" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="50"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="410"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="569" mass="59665" checksum="F8A69DE8DC9B8AD0" modified="2025-10-08" version="1" fragment="single">VSTCTMARTAYIALLFATALAVGWVSNADPEEYYEEYVAQGIQTGTISLFPYRSCNTSNGAYRLAPVWVPLGGGKYCFTIRVNQDAQSCSGACCTSGLHKIEFNVSSSCLVAGSSVTATLNGVPTRVGPSLDKPPLGPVGSTALKITQLGLDPVTAQGAQLCITLKPNRARQGCTTLEQLCSSPGFPAGTCTVATFDVACDCCPVTQVIQARPPPPPPPPPPPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPPPPSPPPPVVPMYRYCEVCVAAKLVPPANDVRPYRFDAATCATIQQNIADAMNAALNVSSISPVFAPFSPNSTNCFNDTVLTCGLFNGLEVSTLESLFSEVSGLLPSFIEVAAGGSVCNPRLEGYSVEITTDGNSCLDVSQSASCFLPPQPFPNCTCNTTQGVLPFLVSPSYYSRSSPFFGSSVTEYCFGINTLPTANIVPSTCYTANDKLAKIEWYTDDAMRSVVKGYTITPAGGASKKVFPSWGALGTSTMKVTLNWTDLEAKGGLVCVAVQKPYTMANLCKGAPGQCYASLFNRDNSDYCCPIFRAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7H3</accession>
    <name>A0ABQ5S7H3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyltransferase</fullName>
            <ecNumber evidence="2">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_009410</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyltransferase 77 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052636">
        <property type="entry name" value="UDP-D-xylose:L-fucose_XylT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032:SF1">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046770262" description="Glycosyltransferase" evidence="4">
        <location>
            <begin position="22"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="5">
        <location>
            <begin position="108"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="375"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI65791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="404" mass="44879" checksum="488256E907616A58" modified="2025-10-08" version="1">MFIRLATALVLSVLVAQGVESKSGNHVAMMIQSHFLQHGVRLPTRAFREAVKNAAINVSHLSHRPSSLIILAMANEEALKRTVPIFLDSLCRVKVSGGTDAGKTFDGRLVLVAWSREAFSMCRELQANYSHHCVRDAEHAAGKKSLGFHTDGFNALGFAKIKYILNGLSAGHDVMFLDADIIMMRDPLPYFYDRGGDMYAMMEKCLLFNETASIAAYEFVRHEKFTPPLNIGALFFKATAGVTRCVYNWIWDMYGQVAFRPQVWDQDIYGVLMPKCSYRFSIRLQVLDPRLFQSACYPRCGCTYHDANVTAASLGKSVAGVPPVTGHLCDPGLWGSWLLRHFPCSGTTDQKAWHMRQFADGLVRGEPVHITKHWANEKKVGNGDKDGRKKVAEDDAGVSTAEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8V2</accession>
    <name>A0ABQ5S8V2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009682</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF123">
        <property type="entry name" value="PROTEIN HESO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="61"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="626"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="906"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1126"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1184"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1293"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1347"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="681"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1129"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1192"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1219"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1303"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1347"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1369"/>
            <end position="1386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1413" mass="150812" checksum="D8F19E69BD275246" modified="2025-10-08" version="1">MASPGIYGASYGTSLGTGSLGAQTSRTQLTVETAIDRNWQFDVGEHKQHCSKDGSEFLEQGLQTLAKSREPGLHDGYTRHATIERLQELLRDPQVFPQPSQLQLVPYGSFLSSCYSRSSDLDLALMGTVSEAVLGRSEGLSAGAPVPLERVTREECVLLLQRLANALESRQLTRGSVDRNPLEARVPIIKFEEANSGIECDVCVTTRGCDFKASIMRLLHGLQPSLAPLIQLVKVWAKHHDINSAHFSTLNSWSLALMVMFSLQTYPGGNLLPPLWRVFHDEEPGGPGGAGRPLQDKNLPLDMMLVVAEARCGDEGERLMAPVWAHYGPPGLLEQLLWFLSCFTSIMNQWKDNAAHRNWRVSTWLGRGYVSRFQKAYLAAVEEPFDCNDNTARSLGIRERNENTLPYVAWVFGHSLHLLRNVKGVEDGARVLAWLFGPEALPSVGLQLLKPSGVSKAFLRRLEKMAGGGRTRNGEEGEAEEEEPDVRMADFKRRLAFRALLDAMRMGSPIMTLDDWRLHHSTRGGRPPFEWHKLAPLTPPLTSPPQPPPLTSPAPPKLNANPQSPYSGQLGVESGFAPRLLHQSMSPEMWRMGGRADQDVGVVRDPGGRVEERRWRSLAEDLDSLFPRPQGLQRGELGSLHPPQSQHQQHEQEQQQQHDLALRRPGFFPPAEALNGFGAPNGSSFASNGQDEVRMGGSNGLGASLGATGAFGGGGGAGAFGGFGGFMNPAVRGVTAIPEPLVPTQRQSLDQLLIPPPPLHQQHQHQHQQHQKDVLRQSTQQVFIPSQQHQHQQQLGPGPGFIGSGLPDGLPQAAGDRAMAGRSILDALRTGSAKEGAVVAAANARDAVAKAGLGGMTPAANSGAGDVANPATSISTSISTSLFGGAAQDSDRPVSAFSFHSGIRVQAPQTQQVPKQQQQQQVPGGYPSLSSPLFSLSMSTSGNSQLLQWESSQSVPQPQPHEQQQQQQQQQKQQQLNLQAQPDQQSQQNHLELQELLRLLSVYPGNQSSNLLLPQPQPHQQNVMPFLGSQPQSAQLAQLSQPSQPLAIATAAGGGYPLAETNPHTRMFESSEIFQNSTASPGLGVYAPVGVDHCPESTPHRPIHHAGVSSYDPVVSPTGLAVQSPQYYASQSPHTSQHQQQQQPRQEQRPQAQAQQQQVISPAGRVGFGLPYPFSSIFGGAAGGGQGGGDAGTSASTVSTSMGSEAFGPGHNGRVHNGQQQPQTQQQQSQLGFGVPGTALGLLPGQARLQPPLPLSSSVPASYSIFGQHQPQLQPQPQQYVSNAFGFHDPKQYPGGLGDGGVSTRNGATSSSTAAANFPAPAPAPAPAQHNFGSVGSTYNLSATASGSYGNTKASPRVSSDGSTKQTGTGTGIAGTGTGSGNGNGNGNSSLPARANGGQTFVAAAAPRRPPGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8Z1</accession>
    <name>A0ABQ5S8Z1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="14093" checksum="A9C4CC9F590D6B42" modified="2025-10-08" version="1" fragment="single">AILRHVKQLLFLLTSGAAHFVGGQYISPLHIVPQGIATMTLGEAYLKDILRPPPTGFMPENVAHPYQKSFYTYATKKLFPKHWYLLAGFTFAVTLYGQLDKLRDAGKKKSYDEAVLAGKQPFTAGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9E1</accession>
    <name>A0ABQ5S9E1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3'-5' exonuclease</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Werner Syndrome-like exonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_009434</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051132">
        <property type="entry name" value="3-5_Exonuclease_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620:SF109">
        <property type="entry name" value="3'-5' EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13620">
        <property type="entry name" value="3-5 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="11">
        <location>
            <begin position="1100"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="228"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="269"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="475"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="525"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="583"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="641"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1166"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1232"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1479"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="533"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="583"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="651"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="666"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="682"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="696"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="708"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1177"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1238"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1490"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1501"/>
            <end position="1510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI65811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1510" mass="156437" checksum="AE65909FA1293533" modified="2025-10-08" version="1">MLSQHLLAEDFEEVERVQYAAERSAQEPAVIGPSYGIPNAGPLADNTNVRASHCAWVAAPHVPTSDITGIEVEPRGTWHHDDVSVAAHSKVEASDSYRTVSPLPVPAYHCSTCAPPVTGYQNPASSYGQEGRTNLNASTPSNCPCTGDGILHGGNGGSPKLPPAKRQSVAPNVEPCWNTAGNCALKLNLDRRQVVFTATVTEAAATTAPSLLTSSEAVPLPWGTSLTSDHLAQAPSEASRARGSYSEGPLQINAKPGFGAAPNASWLQRQSALPSASSAVPPADLPQQAAPPPYPSKQPYQLSKSLQYSHQHNTFGGPHGLHPCHPTFTRQQQGPQQQQDTWPVAPQRQSHGQSPQTWPQQQVEFGWEKRPMTGTAEITAGRWQMHLETRSRRGHTAAQHVISSALGGTYDQLQHQQPQPQPQSLSHPQLHLQPQQHLLMLPSCKQDDEHHTHLPQTVSHSCAISAASTASPVLIQSIPPPNATSFGEGAARVSDTGTGSGSEFVNASMELMRQPSPAPTLICQPVAPDCTGSEPNLNPGSRSGSEIKQPSQFLPQAQPQPQTRPELCLHLQPQYVCPLAHGSQLTRSVQSTRKAPGPPGQRHSHPRRGTQQPMSLPAKVLCVPPPPAAALVGMQMPVGGQRNVPAGGAGPMPLQQQLQGQQQKPSNSEQRLSNRQHIHLPNQYQSLPPSQLSCNQPQKAPEQQQQPPQQPPQHPPSSPLPRHRHHQQPQHGLYQGDMSPRWRAAAPAALPGSPEAKCQLCPNKCALRPHAPKTLDAGLCGDASGARGGAMGPFRATATATPSTTTIARASASFLPGSSYRPAAPEENQCSSCAYPSAAAATAATEPLNAATAANAVAYPPDFCLLHAAVGNSASGVPHGGSSAVVTAAGAAGDGQPGGGIADPAAVAIGYGTETLENPAACVGACVSGIGHCGYRSSATTITTAVSAPVIAAVTTPVTTAVGDPSSGCEEASGRKVVITGFGGTSHIGSAFGGCTGTRGGGPGRRLPASLAAVTCSAPSNTALPPGIAAAASMPAAAKGNPAARGVGCPGAAALAHAGDINTCPPSLPSIAEVRAARAAAAAANGPVAKQPVLQFEGRVRYAATAVEVDWLCRELLAARPPVIGLDMEWRPHYVAGSPANPTALLQICYAVPAGHPGYLALGADRPPSGRHHGQATTTTDNNNNKSTIDAITTDNNNIIIIDAAITAASGCVGADSTAVITSSTTVCGGTACGASEAPRRDPGRDPSNDTDKAAAAAAAADTCTGRHCCCLLLHIIHSGVTPRLRALLEAEEPCKVGVNITGDANKLKRDYGVEMRGTTELDLLANDRVFQHIAHVTINTEYRSRWSLSALVETVLRRQLPKPNNIRCGNWEKRPLDAAQQRYAALDAYAGLAVWAALLRLPRRRKAPLIVPPVASASAPGPELSLEAIAADLATGSNGADGHAILGSNDSSASAAIGGSVVARDCIAAAGHVADSAIRRNGNKVSEPGDAGVGAATAAARDRSPEVDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9U2</accession>
    <name>A0ABQ5S9U2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">IQ calmodulin-binding motif family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_010476</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000037">
        <property type="protein sequence ID" value="GLI66640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051185">
        <property type="entry name" value="ASPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706:SF1">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="196"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="331"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="347"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="491" mass="53766" checksum="D8E197DFECEE9EC5" modified="2025-10-08" version="1">MDYDALSKKRDAAAQRIQSAWRAHRGRKAFLRMRYAANRIQAVAKGWYVRKQVQDQRGRQQLDQRFQEVMEKHREKMRVLEEEKRALIALPGSEMEEYEQRRFIAAVKVQAHWRGLVARRKLAQSPERIRREQAARRIQAAFHDVVRARRSASALASVASSSLSATAAATGAAGSGLSPIRHSPGALRQSAASVLLGSPPMQGSPTSPGDGRSNVSGGGGGITVRSPPRESRGEDATRAGLHARNFSVMGPRRYKELHAQVEGKLTAYVALAKSRGSRRPDPRVAEARLAQLLAQYNRTAPERLAAVAERHRNLVAMDTLCAQLDQVRPLADLPPDAAPQDFPRPARGSERAERAAQSHALSLAEAKIGNQWWTHLRALNTQAVQDALLAEDEERWEALDARFRRKWKDVEDQENRRPDTVEEDLRSKAGVGAAAVAAARRAAAIQSKEDEYAARIARAVVAVPVGAPEPVASRNGALGTSSSPEGAEGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAA7</accession>
    <name>A0ABQ5SAA7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000044">
        <property type="protein sequence ID" value="GLI66852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="360"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="751" mass="77130" checksum="50E091DC2176EE62" modified="2025-10-08" version="1">MDIRAMDAERLLQASKAYCRWPNMMPVRLRGMGTGAYTAKILGGFEFDLGTIFVACECERCAQKPALVGVDVKEEESSIMSVLEFAIHCGVVDLIEHRLCEMDSETVQLAASRMLPEMLFVLDVPEGGGAAAAAAGSCDAGSCTHSFGVLPLSDFLRQSAEAAGGPLLEGRQLGVFVFTSPAGAMTAKDVVSEGAANQLPNTVADAGGSASLSIGRGVEYDAETGALRVKYDTGPLLELCQDEEEEEGEGRKGATDAWIHVAVCHVVLDPDLNPDVESSRRDVDVGGTGHHAAALARRGLCGPFRGCLAAVPAQWDSPRLDPLLQRHLQPPVDNPDAVRSAATAAATAIGALPPSPAQPASGAKEGGATAATAEVPTAATAELGSKDSPATRGAEAADVAATAAAAASGAEEMDTDMLDSGSRNMAGLAVEGLTASLTLAALSDGDDGGGVAAAATGPVAAATIPTGAVEADVEMQLLPNEALEKAAAAGTDSTAAEVQLLQLRLQQLAEHRIGVVGSSGDSGGCRGDSERGVYSAVSAPGEGDVAAPSKQRSASVVAEVAEAEAAAEARRTGPDICREAAQSAEGSGQSVDGAVTVVLTDQAKAAAMEAKRAGRDDFREAELSALLERAVRFRLRKAAPPPPQREEVGGGGLGPSEAEIHNVEGPIYADGEEEEVEEGEGGEEEEMDAEVVALCGAINSLDEAMLQLGLRGPIRTKMLLCVWMMRSWQVMRGRRGTRGALHGGKAELLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAL9</accession>
    <name>A0ABQ5SAL9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="550"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="418"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="708" mass="75124" checksum="9D0EB19C8B00C164" modified="2025-10-08" version="1">MIYDCVLMSPGQQVVTSPSVLAESLKSGDDFIKSSVAASTTGAVAVAQHPRASSATRREEHRAFDRRVSSDIIVTSTKHVAKPWKQERAAIERRGHSQTGEQGGELRLTHERLQQYAAAHARLTAPARPGPRSTAGSTLLRKITNLGALVPSPQQPQQLGPVSPTGAVAAAVVRTISSAPVLSPSALSGARLALTTPGVPPPGMGAPTGARPGSAQRPQSPRQLLQRLVSFPQAPGSLQQQRQQGAPPPERAFGPRAADYPSRHASPRPALPSFGFAASGGAEYSWQPPSHQPHPPANAKQRVTSARGPNSGWPQHLQIAIDSPLGSPAGVAGKGQGYGGWDALHSPGYVEAATAAAAAAAAAALGIRDAKIISPPGSPDRPKSPLPAPPLQQQQQQQQQQQQQQQQQQPKHQYAIGTTNQTQQSQQQSQSTEVLPHLPSAQSHPQPPQHPFPPYHQSHATDAGLPQDGGSPHSPAKDRRLRPWTPRGGRLQRPQSSGPGTVRWGPVQMEGPQPGFAQQGRAGDVVVVGLRDVPPSQLPFARAVMEPEDGLEGGQQAGRVSRSSDNGTHASWWHMPRGDSEASYPLTAVTDRNGLGEDDMTPSEAAMWLSRMAELEAEVLEEREFRRKYEEELRMLAAAATAGGRGSRPSGGGPMLWQGAAMRPRGGPKRICAGLYLSRGTAQCVALLLVFFDSSGRECVCIRKCPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAM3</accession>
    <name>A0ABQ5SAM3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_010601</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00159">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017178">
        <property type="entry name" value="IspG_atypical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004588">
        <property type="entry name" value="IspG_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058579">
        <property type="entry name" value="IspG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058578">
        <property type="entry name" value="IspG_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00612">
        <property type="entry name" value="ispG_gcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30454">
        <property type="entry name" value="4-HYDROXY-3-METHYLBUT-2-EN-1-YL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30454:SF0">
        <property type="entry name" value="4-HYDROXY-3-METHYLBUT-2-EN-1-YL DIPHOSPHATE SYNTHASE (FERREDOXIN), CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04551">
        <property type="entry name" value="GcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26540">
        <property type="entry name" value="GcpE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037336">
        <property type="entry name" value="IspG_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IspG TIM-barrel" evidence="8">
        <location>
            <begin position="18"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="IspG C-terminal" evidence="9">
        <location>
            <begin position="569"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI66671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="679" mass="74897" checksum="9BBB73361253AA40" modified="2025-10-08" version="1">MLLNKYCESVFQSQRRPTRTVMIGKVPVGSEHRIALQTMTTTDTRNVQATVDQVKKCADAGADIVRITVQGKKEAEACMRIREQLFKDRYDVPLVADIHFQPTVAMMVADAFEKIRINPGNFVDGRKSFDVINYDDPREFERERELIEETFTPLVLKCKQMGRAMRIGTNHGSLSARILSFYGDTPRGMVESAFEFADVCRKHDYHNFVFSMKASNPLVMVQAYRLLAEEMYKKAWDYPLHLGVTEAGEGEDGRMKSAIGIGALLMDGLGDTIRVSLTEDPEYEIDPCKRLAGLGEHAWGSGLGVAPFTETTRDTHTFKRRVGDLPVQREGEDLDFRGALHRDGTVYSAVSLADLKQPEITYKRLGAKLAVGMPFKDIATSDSILMREVPPSSDKEGRRALRRLQEVTTHVIAPLEALERDPLPGAIALVPLKQAAHGSVTLPEGSSRWAIEVDGTESEEQLGALRELGPIVVLLNVADGVSRLHASRRVFDVLKRQNVTAPVVHNIRFPAGTRRDEIVINTGSLVGGLLVDGLGDGALVECPGEDLDFLRTTGFGLLQGCRMRNIKTEYVSCPSCGRTLFNLQEVTDQIRQRTGHLPGVAIAVMGCIVNGPGEMADADFGYVGGAPGKIDLYVGKEVVRRAIPMESACDQLIELIKEHGRWVEPPVVEEEAKELVATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAN4</accession>
    <name>A0ABQ5SAN4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006074</name>
        <name evidence="3" type="ORF">VaNZ11_006108</name>
        <name evidence="4" type="ORF">VaNZ11_010200</name>
        <name evidence="5" type="ORF">VaNZ11_010206</name>
        <name evidence="6" type="ORF">VaNZ11_010207</name>
        <name evidence="7" type="ORF">VaNZ11_010220</name>
    </gene>
    <organism evidence="5 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="5 8" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="148" mass="16140" checksum="1543F0373DC31EA2" modified="2025-10-08" version="1">MRFLKPPMYGSCWKTMTTRVAVFSWRSVSRFSVFGRDASTAATSTDMFGRKRRRYSDGGNDSASEANNSLLAVTDQQREQLAAPDGGEDASNNNISLKSITALHARKTTLMRLAKPASGSGNRGSARDHVDVVAQAVVSPWTTLGRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAY2</accession>
    <name>A0ABQ5SAY2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39113:SF1">
        <property type="entry name" value="MEMBRANE LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39113">
        <property type="entry name" value="MEMBRANE LIPOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="222" mass="24686" checksum="1BF5E2299B65CEBB" modified="2025-10-08" version="1">MACSCARASGISMRIAIFFNLTIGVALIIFGCKVQNPPKDFAPVMFVVLGTFNLFASVLGFWGSYHKKRVLVGFLICGGFSTLLQIALILALLFAFESVAQKIESPKENRAKYDKVAAQLNVARWVVLGFVVVEVFTLTMAVLLRFVIKEEQPYNSFDAETAQQRSSTLSSLARDIEKFASKSKTMGERAYDKVRSKMAAKYGNYSSTDADWKSKAKISWRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB23</accession>
    <name>A0ABQ5SB23_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_011303</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF37">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE FTSH 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="542"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1014"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1026"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1098" mass="120144" checksum="DF369B27AB3F4516" modified="2025-10-08" version="1">MQCLAAGSAWARACNPCRRCPAPVLAAPPHVHHRTSLVLGISSRRSTILPKCRHATPTIASASREDRMQEPSQKPSFCGREAVQKSGLDKFLLSIAKIFGVAGLVALTSLSGPTRAAHARDRAVQSPLPIATLAHHRNPVLSDLAAAAVTTAAAEAAANTAAVAAPADFADMPGTHGTVETAYKAFLERVTAQVLADNPGAQGQPELLKRLVRARALGTPLSFDELMRSRVPAPDDVPNRNSRQEVAEQVKAIMDQYDREDFDLGIKQFMLEAKVKAKLEAASRGTSRDRAAPKDYEEALAAELFAAEAGAAPKEKAKTEDMVDDAFTTEVVEEALALFGDANSVKTAWRTQDVLRELSYTQLWSLVEEGHVARLRFFGQERNKVMVTTKASAPGGVRVSKVVLPPDPELLDHLVTHGVVVDEGAPSEDDRLRASLFIQILRYTVPFMVISGLFWILHTWILDPVPNKFRRQEFIRYRREMLHVASKLNFRTPAREVRIDTTSPDFIKWDDINGIDEVKNEITEIIEYLRNPALLRSRGVARIGGVLLAGAPGTGKTLLAKAIAAEGGVRMFTCSGTDFYDVYSGVGARRIRETFDRLRNSAPAILFIDEFDALGAARGAQASGDESASIINELLVQMDGFEDNRGIVVLGATNRPNAIDSALIRPGRFDRIIYMPLPDAAGRAKIMQVHARNKAVDPTINWYEVARAMAGFTGADVMGLMARAARMAARQGREAITEDDIYAAMENKTVEAMVENTSAGGGGGFVADDVAPDNPEPIPPQLRKSISVYEAGKALVAYITPEYEEIARVSVCPLNIISGFTLFVEDEDKNVNAILTRSELEARMVVSLAGRCSEKLVMGEGNVTGMGSPDLFHANLIAREMVMSMGMGRRTGPVDLLRVMGAAEGAPGESTLRAGPEVSEEEPFYYHTSDISTEQARVALSEVVELLEAAEAKAMYGLAMNWKPFQALTQALLDRGTLTGKEVAHILETNGVIHFPDPYTGGFGWDPDGSLRYPFKPETPVPTEEDLQRAKRKREEEEEAAQPKLTGALAKTWFAGTEYDAPRTPDGRFAYGWHWNMPYSIRKTLPDWYKKELERYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB49</accession>
    <name>A0ABQ5SB49_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF80">
        <property type="entry name" value="GRIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="653"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="728"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="190"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="298"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="368"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="493"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="668"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="751" mass="78921" checksum="6525A6EF312E3248" modified="2025-10-08" version="1">MHAMAGQSSGASGNSAASASAIVSGTRSAGGGVMPSPSPSAAAAALRTVSATVGGAPYSSAPMPPLHAATSIDAKADLASGLATAAAAAPLGSGAAAAEAAAALTPPRSAAERMYSAKQLTEALRSGLQAVKDQLQVLAILDGTALSWPGVSQSVDDLDFLTDMVAQTTNRLEAANRGLQVQLDGERAESASLKESLATASNRNDDLQRQCNVVHRDLREVRVQLEDTKRDAQQVRQELWTVEKQRADAASELERTKAELEQLQGELRELRAADAVQAAATTSLKEELATRRADVGHLKQLTGELRSANEHLRQQLTAAQEAAAAAQGTLSHVRSEKLHYEGEVGRLGEECSRLSAELDRNAREVVRLKEWQDVLKKVEAGKLREEQEAHARTRAEAEKKEALWRADVRAREDRLTSRDAELDTQRKANGLLQLQVKDLQKQLSLCQGERTAAATARDEHHATVGKLQAEIQRIRLDAAQCDKERAVAESAAKEAAAADKARLQAKLKEAGREATLTRALSEGWQRELREARSRISALTNELEAVRSQLSEGKVEHVRSGAAAEGQVVALRVQLAAREEEVRRVKLDASSAQKQLEKAVERLQREVDASAAEVARLKGGEDAALFTRLPPVGRPATIHDELEALRKALRDRMAGPMQGPTAGGGGAGPLPMSAPVLDSHSPLTSPTGGGGGGGSSLPALVVGSFGTAVGNGLGGGGGSSASAAALVMTSGAGGGGGNGVSERQPHLRNRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBS1</accession>
    <name>A0ABQ5SBS1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD_REPEATS_REGION domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="522"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="858"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="973"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1171"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="427"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="880"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="903"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="937"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="973"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="983"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1008"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1302" mass="132994" checksum="9CE105D59414431C" modified="2025-10-08" version="1" fragment="single">GAGAVPVGAQRTPEGPAGGGGVGGGCRPTTAVVPAEALTVRQRGNLQVLHRSLCTQATWLMHLGVVEEAAWLLLMRLVAALSAAKSEWLHGVRPGHLPQQLLNVRSALRHARCEASLQQQPLRLHMLVKALGHCCIGPGRLWMGFGMGAGWGQPSSPPHCWPLKAGRRTQPLGVYWASPSTPPKRGNYHSRLKPSGQPMLRQQQQQEHKEQAQDEQQQQKQQTCQLRVLSPPLPPPQQQQQASLAGRGRSLRLSLDIPFNSGRADCRAEGEYTDPRVARLISTPTATTARHAGGSAGNASVRAASGQSPESFPATPTAFRASFTSAAGINAVGGGAVIEPVIKRERRAANTNTGGPLSPTSSTANKGSFRYASAGAASSGGSAGNGYSVKYERHLAAAAVAAAAAAAAALPCRARLASPGSLWTGSQYHQQQQQQPQQAGSRPNPASRCSVALGSGRPIVALAAAGGLVLAAPQEPACFVLSCQQDGSLRVTAKLPLSHLDAAYADKLAISPDGGTAALSVAPLTMPPRTPLPTPPRLQPQPVQQHRPSPACSEPRQVGAGALPAAATAAAAAAAAPDTIRSRVLAVVDVRAGRIVRELQPASHSRINALVLSPTANLLASGCAASWARLWDARLSRGGGGGGGDGGGGGGGGRCAPVVSLATRQGGVSALLLDSCRLRLYTGGRDGTAAEWDLRKPTCPVVVYGGHNGWVTSLELLTPSTTLTENRHVERCWGTVGEISTTTAAAAAVATASTAAATGTCASPHGHDDINLLRALDPLTYPVENWRTPRRVAQRPMGSCIDLYDVVLSWPSPYLVTGSTDWTVRIWSPPAAVVAADGAAGAAGVNCSSAPAAVADTAERSAVPTPGVAARPRHGRTRSRLGDGDAGWRGDDFSNVDDTDTGVGVGVGVGGAGNGNGNGEGGAWWSTEEGNRRNRGSSRLSPPSLSSPFTGWAPGAGGAGSCRGGGSDGGCGADHGDGGGGDNDIEVLDSSDDVCGQSGGHNSDTRMDGGGGPGGGSAGGGGDGGGRSGVKPPQPASSPSWRMASPSPSSTSTSTSTSTSPAPSTPTSTSSSWQQQRQQDVRYCRKPRSVLMGHGGAITALYVDKSAGVGAGRCGDAAASTATTPATIYTGAKDGSVGAWTADGHCEQLFPAHRGAVTLLATVPSPASSSLTSNNFAVPTSPHPHPHGKSSHHNNNNNHFHIDEMSTDVKLRRPAGTQPAGGCAPKPDQAVPLERLLVTSGAEGSAGLWALTHGSLGYSPLLDRNETGSLARVAAWDAARGVLLQGDECGVVRAWVPGERRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBY3</accession>
    <name>A0ABQ5SBY3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_010995</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000054">
        <property type="protein sequence ID" value="GLI66949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF8">
        <property type="entry name" value="SD21168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="118" mass="13436" checksum="4AEEA82429A18B38" modified="2025-10-08" version="1" fragment="single">VTFTNHFVTTPICCPSRVSLLRGQLGHNTNFTDVLGPHGGYAKFRALDLDREWLPTWLQKAGYKTLYTGKFIVDYTIRNYDPPPSGWTVFDALVHPFIFEYYSPAFSLNGGQPRMYPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC79</accession>
    <name>A0ABQ5SC79_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010759</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046774245" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="44"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="58"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="283" mass="29550" checksum="58571BEBC128A3C9" modified="2025-10-08" version="1" fragment="single">MIDSNQGFALTAARMCGRFRPISGVCFAALAMAWLSAVPTTVADSGSGRTLMTTYDFPPLCNCIRTLNAAPFVLRASSASSVAGAQRFCFRIETSSFCDPKFKCCNGNQGVSKIEIDVASTCRPSLQKVTLNGKPTSYEFNAQLSVLRINKINAEAAKAPNTTVCLFLSDGTECNTLDTFCTIGGGVCKYALFNTAKDCCPVGLAGFTQPPPFNITPAGTHSPPPPPVVPLPPPPEVPVPPPPVVPLPPPPVVPLSPDPPSPPPPSFPLPSPPPPSPSPPSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCK0</accession>
    <name>A0ABQ5SCK0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011885</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="53"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="86"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="152"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="185"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="241"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="322"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="497" mass="51576" checksum="BDD865DD7F89930D" modified="2025-10-08" version="1">MSVEVRAKTRSKPNTVHYAAETGDIKLLEHFVREGMAIGNPESVTLQSREDVLGMMPLHVACENGELEVVEFLVKKRVDVDAGDNFKVTALHLASIENHADIVEVLLKARADPKPADVEGDLPIHWAATKGHSLVIELLARKGSPIDPPNKKGWTPLHRAAYNGRKDATLALIKLGAKISGTTSDGNTPLHLACFMNQLGTIEKLCQAGASQRLLNKSGQRPYDLCITDAARDILKSLQGFEGDEPAEGPAAQGAHAAAKPKDASVASGTRGKPVVPPNLAALQISGSKKIAHDGFPSPVGSFTAPRSEATGDAGFIQTALGETGTNPYTGMSSGVAGSSNRMSGRGGSVGGGAASGLTDLGRPPSQGVGSSPAGPSGLAAHPTPLAGVLRPLRAPSPGGASSSVIGSVNVGPLRPRSSGLTGSSGIGEESSQWTSGMAGSLSPELLSEDLQDQIKEENRRIAASNDVLRPKSARPVSANKKFLARYNLDKLNLFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCQ7</accession>
    <name>A0ABQ5SCQ7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000068">
        <property type="protein sequence ID" value="GLI66966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="422" mass="43303" checksum="96D245EFB072B50D" modified="2025-10-08" version="1">MLSVVVLSAGHPVGCGAVGLSFCRLWCCQSVLVSVVVLSAGHPVSCGAVSQSLCRLWCCQLVILSVVVLPVGHPVGCGAAGWSSFWLWCCQSVLLSVVVLSVSPCVGCGAVSWSSCRLWCCRLVILSVVVLPVGHPVGCGAVGQTLCRLWCCQLVILSVVVLPVGHPVGCGAAGGSVLVSVVVLSVSPCVGCGAVSWSSCRLWCCRSDLVSVVVLSVGHPVGCCAAGWSSCRLWCCQLVMLSVVVLSVSPCVGCGAVSWSCCRLWCCQSVLVSVVVLSAGHPVGCGAVGQSFCWLWCCQSVIPSVVVLSVGHPVGCGAVGRSSCRLWYCQLVILSVVVLSVGHPVGCGAAGWSSCRLWCCQSVLVSVVVLSVGHAVGCGAVSQSLCRLWCCQLVILSVVVLSVGHGLSLVPCAMCHVSCVLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCV7</accession>
    <name>A0ABQ5SCV7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Borealin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="38633" checksum="6B660379B3EB72E5" modified="2025-10-08" version="1">MPRKRKAQHGVPLISDEAKRGRNKQRIKNGDDDALEDKNEGGEEEAHDVVKICKEQVDLKVEAFVDAVQQRVQMLRADADAIIRKIPKQVRLMPAVEVFALTPTESAAGTLQDVLSRLQALQQGSHDSKIVDIARIVSASLAAVGEAVPAVASAPQPVDAGDPCAAVQEGQEQQQEQCGTLSSQAPAASPNKLAQAAVLMPPPPARLPLGAAILPARQPRVDEVFYSANGSPIHFSQVTAPVTAVKGMLSCLPDMTPAVGANTVIAPPTVEITGCTFRRMAPTRGRVKQPTRAITVKTKDGKQFTVDDLIGMAAVPQAYHDEVRQLLKADVQDLNALMATLEQDNDNTSSATAGASHPTRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD65</accession>
    <name>A0ABQ5SD65_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011924</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028061">
        <property type="entry name" value="Fis1_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028058">
        <property type="entry name" value="Fis1_TPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14853">
        <property type="entry name" value="Fis1_TPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14852">
        <property type="entry name" value="Fis1_TPR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="183" mass="20289" checksum="35C25905B6E8130E" modified="2025-10-08" version="1" fragment="single">HLPSVDLDFVKHAEDEYHSIFNTGKSEEVDAARFRLVWALVHSTHRGHQSRGLELCRAKLREQAKDKEYRYFAAVACYNMGQYIDSRRELSSLLQVSRRGLTRCRFRLCFTASQSGFGWGTSRLPPGGVPAGLGGGRYCARGAYGGGDRGGTGGSGAGHLLRIWPEALKQGCRQGWRLNKAMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDH2</accession>
    <name>A0ABQ5SDH2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011955</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045278">
        <property type="entry name" value="CRS1/CFM2/CFM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846">
        <property type="entry name" value="CRS1 / YHBY (CRM) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846:SF7">
        <property type="entry name" value="CRS1 _ YHBY (CRM) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CRM" evidence="9">
        <location>
            <begin position="311"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="40"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="152"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="282"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="890"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="893"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="903"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="972"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="972" mass="107427" checksum="E69F3E26A55435C0" modified="2025-10-08" version="1" fragment="single">MHLGRLFPRGFASLNLGARRFLASRLTPSRIVRVCVAADGNSDDLPKQEHGEARVPGSRRTLFQDAAPPAPTESTTSMRELLDIHAQSLQDSGTNLETRLPIIPTHWDLHVQSPLEIQENRLHSVRTSLRRETRGPARAAVSREFRVAERRLHSSVRRPRQEEQVETEAGEDHLGGRQQQRQQWVAAAAKEYGDGMWSPKRPKTTNRKKSKEPSPEAKERAAEVAARNAARYVAAHTAYKRQKAVAAAAAARSDDGDDDEEDAPEGEMEGEEIFSLENQIRDLQGQPGGRGRQRDFTEEGGPLQGPKLTHDLPPEVNLRLLQTILMGSRNASMPEIPLGTSGITNGVINAMFLAWRGNELVKLRVRDKRKVAKKYLPYIRQVCDLIESRSGGVVVWRSGRSIWVYRGEDYTPSEPGKLKEELLGKTEVPIYFQELSAKQPLLGQKVTLPQGTDGYLTRGSAELLVEDPAGAPAPAPPQGLGVIFLHDAFGAGSPNTQQHCKQVCDMLASVVGPGTPVLMPDLTMGDKDIWRGPVWPPGKPIFERQNGWLANGARGNPEAVAPYVEAAVSYMRGPEVGASRIVLVGTGWGGWLALNMLAADATKLQIEAEVEAEVKAEAATGAAETTPAAAAATTTIGRTPAGVKGGKVAAGKSAAPDPRMLGKAALHMMEVMKRELPEEAGLLQRGLWMLYGPEAKGYRQAKVLARYGGRRPRQVDPDVYSGDGLIFSAGVVGSPSHFFGDEERMALAMSVPLCFVCSKWDPMEKFQLYMMPPLVDQCAFKRFGKITPGFWGKQAVWSDPVQARQATQATELAGWFLRWHMANDETAQERVRKFCMMGKSGVGEKQTTNLGFPWLEQLLLYRGHEKQLEQEKKLKRQELGLPEVLTKKDLAKKARREAKKKRDAAMKEQEAGREWRPEREAGRPGGRRDDRDRYDGRKGKEYGGRDDLTSERWEERGEDGGRGDWSRTAPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDH8</accession>
    <name>A0ABQ5SDH8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011570</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14014">
        <property type="entry name" value="STKc_PknB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="591"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="941"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="631"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="850"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1192"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1272" mass="137384" checksum="4EA2A7F3C7B0F9E6" modified="2025-10-08" version="1">MGNVFVVGNGTSTWDVSASPLSFADNSSVTVSCLRILLEQPGPEVTQPLPINYMTLLSPSFIRRSSKTQLFALTIRDVEISLSCAALLEWQHYLCGVQLPGRTLVHGNTISILSWMGNRTLLEHVNMTCEPGTAGFSPACSMAVVDVVSDILDASSRLQDPYLPLVLTVSRDLSFTDSHWPDNGLRITANVTLSATLGSNVTISFGLRTVLFTVDKNSSRTAFLSFRGCILVDLPLSYLPLNHPRRPFGVLSTALWPVYREEVAMGLYNCTLILTQEEFKFWSYWLSILVSPIPGVSVMGNWTFANDVQLGKSGIDYIHLVNYMGRYTTLMNVRYQVSNDVYVTQQRNNILQITNNEVVPPTVVFQARDSDDLLTALSSPSATAAQHGQYIILTGSVTTLDDTIWPVTGAPIWQPTIITTWPSSWVALDLRLIKDAFVIMPSSLSSVLIKNVVLLNLCAGDSVDEFAGLTTGLWAFKFTNWRNTEQQRISLDRAVVVVSREEVGYYIDATVNQQKTDAWISSFQVDSYDTTNRSWVKFATLATARFSAQSVNITNTIPDTLDSDSINLTIVQGFPWNSRVTEQPSEKKRDMLMFALLVITLPTALGAALLTFLLMKRRRISRLCREGVSQRKLAERHDDDGEEDVSDGEEHEGETPRKQEPPVCCFPLSFQLRKSKFVNPLFADAGRAPAEIVLTGVLGPVALARSLVPVPPEASMLATNASTSTAAGSDLEFDGIAATGPELLIKQAMTENASAGTRSGGGSQALGVLIAVPTELRRGAEVAGDLAAAVTGSGVSPLSGSCLSGSGPQLEHKQRSSCCPQLASADGPYVAAAAAAAQSAPGNCGVIAGSPHDRLSSDGGGVGSSSPQEDEFRLSPAAAAAVLATGQAAASGCGEDANAYTQRQGLLGGTREASQGASRNPRFLDMQQLDRLRHEIGDRHLEVQHSLGWGGCGVVYKGVWKGLQVAVKTILLQGDSKQSQQILTEAAISASLQHANIVTTYLYELRPLDEVVPGTGNMEIQLRSTSSDGVQPQDWEHMGCWKLYIVQEYCELGTLKAAIDQGYFKSLAGGSPNLAFMLTVALDMALGLEHVHSKNVVHGDVTVSNVLLQACPSRPQGCIAKVADFGLSVKFDTSQTHGYHLYGGTPHYMAPERVRGVLSKRSDIYSLGVCFWELYCGTPPWRRNAAGAHHRGYGGGTTSSSVDSGGHHNQHLHPSFGDSAPGTSLSDSFRFPRGCPPEYSTLVHACLSVDPQARPTATTVASTLQRLHRRYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDM7</accession>
    <name>A0ABQ5SDM7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="101" mass="11810" checksum="F6A6035DB944C6F9" modified="2025-10-08" version="1" fragment="single">YHWCCYGFEFVGKAFVAVAARDLPPRVTRQDRSYKSRARHMDSFKQQQGIPGNISIHYKPCVSPARHSGYRQPRDAHLRHLVRPVRYSGHQLLQIILRRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDV0</accession>
    <name>A0ABQ5SDV0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_011876</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="14"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="14"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="126" mass="13781" checksum="244812BFDCCAA48C" modified="2025-10-08" version="1" fragment="single">IVTHGLLRFLQNSNHGAKPCVRFTQRGYCKYGDSCIFLHVPHKTLSSASGRSGFDTERTPQLGMSEVTMANNPPETMPTPQPPPASRLHLAPAGPAEQAGPAKVLFRAARDAIRRDDFNVLRSTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEE2</accession>
    <name>A0ABQ5SEE2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Autophagy protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011970</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for autophagy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Conjugated with ATG12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ATG5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.190">
        <property type="entry name" value="Autophagy protein Apg5, helix rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007239">
        <property type="entry name" value="Atg5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048940">
        <property type="entry name" value="ATG5_HBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042526">
        <property type="entry name" value="Atg5_HR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048939">
        <property type="entry name" value="ATG5_UblA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042527">
        <property type="entry name" value="Atg5_UblA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048318">
        <property type="entry name" value="ATG5_UblB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040:SF2">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20637">
        <property type="entry name" value="ATG5_HBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20638">
        <property type="entry name" value="ATG5_UblA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04106">
        <property type="entry name" value="ATG5_UblB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="3 5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Autophagy protein ATG5 UblA" evidence="9">
        <location>
            <begin position="22"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 alpha-helical bundle region" evidence="8">
        <location>
            <begin position="136"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 UblB" evidence="7">
        <location>
            <begin position="592"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="216"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="334"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="533"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="252"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="640" mass="65345" checksum="C0BFD6C1AD4C2694" modified="2025-10-08" version="1">MAMRIYSTDGKTPMKQVQAQTWHTKIPVHLSLAPDNISSPTGVRPIYLMAPRQGYLHVVASQAWPHLQHVLPSIPGRPGPPRPWFDNMGVPLKWYLPCGVLYDLLADLGQLPWRLTVHYTYPPDILVGWDTGATPQAQFMNSLKEACYICRGTDGSGAVMRMGSTAQEELWSAVQAGNVASYQSALEGLKMVPTPRQGHPPNIPVRLLLRRGGASASASISNTAPTTRDALRSTSVGDVSPGPPRTGLPADAAALPSAGTTSSPSGAAVTMSSLGSGGVWESCIMSTSRPVPAQLGSGGELTTLAHLLHVVLPHIFPPPPRSASAVVEVRTRPAPQVEAMTPSPSPSPSGATEEASGTVQVVGRLGQDRDRDRDRGERQELADVRAEAISSERGSGGWLGPVGEAEGSCVAAGQGPTETTEAALGGGSAARSPGQGATVTIPTEPGGAGGGNGGSAARDARAAAPGPGLEQSVEQISGEHRSRPVGVGAGIADEKVACGVRAKGLACSEAVVSAAAGPLREADLVAATSKGLGTWESRETGNQSGQSVEGGGPSVPSPDVSAGPASASAATMAAGPSGAAGCGNSVAASWPSYRILVGGITPPLAAPIAWLHAAMHAPDLFLYVVVRMDLEDGSLVEADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SES0</accession>
    <name>A0ABQ5SES0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012803</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="196" mass="19940" checksum="57C9785BC3EF69DC" modified="2025-10-08" version="1" fragment="single">ATAAAAGGVLEGAAVGPTGGGALWPQECPVAGVPRFVVAEPPNPHQHHFGANPPSHGDLIGGCGGHGDGNGEGGVGGRVMVGGGGGGGVNDPIRGAHRIYAGSHYEQFNTLGAILGPMGWSWDMDTGELPPLSLPLSMPRLPPPPPPPLPPPPQSLPLPRLLLSPPPSLDLRRNFMPYSNDDIQMGNDLITSDGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF44</accession>
    <name>A0ABQ5SF44_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptide-methionine (S)-S-oxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-methionine-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012785</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050162">
        <property type="entry name" value="MsrA_MetSO_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799:SF2">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="8">
        <location>
            <begin position="21"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="142" mass="15424" checksum="4108986BDD20A743" modified="2025-10-08" version="1">MGEDEPCRLAPEEAPPGLKLATFSGACFWCLELGYQRVPGVITTSAGYTGGSDPAPNYLSVADGHTGHAEAVQCIYDPNECTYDQLLDTLFEVVDPTVCNFHDGKQYRTGIYFHDEEQQQAAAARIVALNDQLKAGTAPSHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFF9</accession>
    <name>A0ABQ5SFF9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012453</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="11722" checksum="D126378D95060C1A" modified="2025-10-08" version="1">MVSLLNNVQMKQLPSILIINTSEWHIDSSLLSNNRCKAGPNQCFRDHIAAPWHLARFASRSQLESGGCESSNAMGSPSTRHDEPQSPVEGCISSSPAWLKKFSISTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG02</accession>
    <name>A0ABQ5SG02_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013395</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="entry name" value="NusB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054728">
        <property type="entry name" value="RsmB-like_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF61">
        <property type="entry name" value="NOL1_NOP2_SUN FAMILY PROTEIN _ ANTITERMINATION NUSB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22458">
        <property type="entry name" value="RsmF-B_ferredox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="NusB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="343"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="612"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="437"/>
            <end position="443"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="489"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="559"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="686" mass="73079" checksum="1F2BF3832DE36EFA" modified="2025-10-08" version="1">MDGGDINRNRNRGRILDQDREWGRDRDRDSSGSRGWSDERPRHDREGGSHKRHSASVSDTGSGRSSTKNGTQRQDKIPPPQPTPVDRKMARSPRFLAVQQLVRVEEEGAYVGLVNGSPVAPARLRDKTNRVEVDADGEGNGNGEVDMYGGDDFTASRWRRAEGGGGSGGGRREAPLDPRDQRLVKELVAGVTRFRRRLDYIVSNLSQQTPADMESGVRQVLRLGVYELTERHLAPYALNEHVQLVKAVSGPGAGSFVNGVLRSAARHVEAGTLPTPESRTAGAAGSSSSGVDGGGGGGGGGGGGGNPGGGREALRLLALQHSHPNWMVARWLERFGQEGAEQLLRRNNQPPVYTVRVNTLRPGASAARLLEQLLTVGVQAEPSPLLPEEFIRIRSGLQKLLAEGFVSDGSLFVQDESAGLVVTLLDPQPDEKLLDCCAAPGGKTLFAAARMQGRGSITALDISAPRLGALRRAAELAGVSGMVNTHACDLREWARKAAERAAWLAEMRPAAPAAAAAAAATAVLTSGSGGAAALPGSRESWSQLGQPMTPSLYDKVLLDAPCTGTGVLAKRADLRWRRTPEQLRELVTLQDELLDAAASMVAPGGILVYSTCSIEEEEDQQRVRAFLDRNSDFQLDLVTCGTAGGPVNPPRPGVPPEVVTAEGFVATLPHVHDTDGAFAARLCRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG18</accession>
    <name>A0ABQ5SG18_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PI3K/PI4K catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047443296" description="PI3K/PI4K catalytic domain-containing protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="536" mass="59443" checksum="C002AF5413082A42" modified="2025-10-08" version="1">MARDGGIIAPLRVVVCSLASLCIFWAASIAAETLPHINDCYLCQKCHTGKPSGRRMLAQGSFRGADDASASRRLASSTDTSAAETSSVDLQRGLYALASLSAHAGGSGRSLRAQAQTEQMYVPADFKFQIMPLKNEIDMKSSKPNCTECYGCDTQIDPLQGVVTAAHGQELRAEVGASPEWLFLADLPDTQTTGKRAVVKVWCMPVDKVHGTFSWRCGNTGEGAKAVQFLLAQQKVMEECGLMDVTIKVWPGRVNAVLPGHGLHVWWDGLWMERAEGLSLNQISYKRTRNFVTGSLKTVMGEQLNRTLIVRAALYDLLTSQCDRHSQNVFITESGQIKLIDNLQALQFSWVNCAMDSIFLPGSQKNMVIKWGGNVAFKIRHAQVRRSINPMVVLDYRCYVEGGRIGKNYDPKMQQCLTKLRDMTPKQIQLEYGFPYVSSAAILQQRASDMISKGFEWTLRYGKPRSFPPKSYRPQQPCCGLNVTPKGLLHCAHPWPNRVELPYGDPQSGGEWHRPYPDPGSHVGGTFLDFPQEGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG97</accession>
    <name>A0ABQ5SG97_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013165</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="402"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="519"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="605"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="646"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1225"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1338"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="540"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="670"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1374" mass="142601" checksum="CA8C026AF38B0B6D" modified="2025-10-08" version="1">MERYEYIGQAGSGAYGQVWVCRVRPLPDGEEPIDGLRQGMLVACKVFRQAHTDKEIMRLALREVQVLRAIKHDNCVRLLDAFTSKSGKVYLIFEYVEGCAYKELCKYRTGLPPPALKLMVFQLCLSLRYLHGEKIVHRDLKPSNILINNDGVVKLCDFGFARFTSCIDPGDAERLSTYVITRWYRAPEVLLSLEYGPAADIWALGCTIAELAVGEPLMPGTSTVDQLWRIMRVFGPLPPHMAVQLVQDPRLAAHCVPPRGRTLAMRLPHAGARLVQFLEACLRLDPTQRLTADELLRMPYFFEIPQLVAGTTLEQLVARNTQTRGLTPRRRVPPSPQPPVTGVPQPQLQTVITKSAPRIVSSSSFGPQVAVAASASADAGVSAAENPKMRFLQVVHVGSLNEQRRKQKDHHHQHVPGAEEEEREGEGENTAKATQQEQRESVAAASYVATVRAGTASAIGSASATGTCNTTSPQSISAIMPTTPGVAVAAEGGGVSADAVIDGKRALDVTIAQLQSVSCPATSDNDLNELRLQPPTPAENHQDREKSTEGSDAEASLADTAAKGGNSTAADAAGAGAGNPGSVSTQLLSLRNVVGAVAVAAAAARTQHPTMPSGEPLRGLLPQLDVAGGSEPRRLQHQEAITDFQHQEAEPQHGWSPPSLQIGLAPANGGSAGGGGSGGCTSQEPSLAQGIIRGYSQLQDAAAAMSSGARGNPEAVLAALLYGSCSRQPSGGGGGSIGGGSASLHAAARVLGAMEAQTSSASVAAAVTMTHGSVGERIGLGSDASYPSLRGWAVPANQIQNCLALAEALACTDLSTSLILGASAATATVESMKNSIELQAPRVLQNQDELRALELSGCAVFSEPKESTRQQTSSATGTAMTASGDISGVATVVASAALPMTAFGAPEPWVPTGPKRRIFATGRHRNVPHSATVATSFGGSFGRRHQPDLVISASQQQDLVAAGPQSCSDHIGGDAATALMIPDANPAAVVAAAGATLSGVAPLPLTRTPTCSIALSTLLAGDLGEVMTPMGAPQLPSCTGRTYPHRRPPNQVGAVYDFTMSPLGSGTLSPYLPATGARVTASSQLDTKPTLTSSAHLLRADDPIGELNRSTTMCSPFGCSTIDNATSKAASAYLAASGVGGGSGTIQRSCHLDAGEAEAEAEAVLSDGRLPAVSSGRCTAAAQAAHRPRSQGSSLFPKAAAALLRNRRDLAQPSRCFSDRNPCTAAPVSDATSEVSSGKGDMAPGYVPRSTAPGMTSRCAMITGSAPADPAFGAPSGFSGAMKAPLGLTAAVSFRSVRSCGGKYNKENEQRQQPVEMGRERVRMGDPAGHACTAIAAAGSRHSSGGDASAVTTGKDGKGGLLQRARRKLQRLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGI7</accession>
    <name>A0ABQ5SGI7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="674"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1014"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1014" mass="104085" checksum="66E198BFB936073D" modified="2025-10-08" version="1" fragment="single">VAAAAVAAGVAKPSARASRELRLLEPFGWDRRRAKHPANLDLLGVPRAGRQSVAAAAVIRSGGGGGGSSGSGSAAARATGQSTGNGGAAAGGQQTPSYSGGQWAGGTATAAAASAGGAAAHAATVMTPAGNTHRDEPEHGQQLPQPQIQPQQRNMAAHPVASAEMASLSDNVGFPTASTAITPMAVDAPRTGGGGGSEYGRQYSRAGTEDAAAAAAEEEDEEDVVVMRSGFGAAWRQRGSGSGSARRRRAVVLDGSEEQDVMELPLPPPLLPLPLLGTPQASPDVRPLVQPSANGRTEPSGPDKRSARSPALQDRKHPTHGSMGVGKTAPIEIDRREVDHGRISGATGEEMVLEQVNDGGGPSTTVRGVGERDIEPRTSRGGSRTPSLDLADGAVSELPPPQQRPQRPKPPQQKESQQQQGTEGAEQVLRNLRHEAASLRDVLLMLRARRPQPQQTTTAAATTATTTTVAPQQPNGTSVATESEGEGHRDQLIPAEAKEEVELQQRLLEVESRVRRAERALKQMKPWDWDELKDVHPPNVDKLAPAEAKRQRRMPKHYSPAKVYAEAQMLRTRATRGGSGGGAAAVAVAADGDGWELYDPAALSPVVQNQKLPEIGGRGGNVGNGTTDMGLRNSSGIKRSAHGLADGNNSLFQMRSPVKRQRQSAGGGDTSNKGSRGSIVSTSTSTSRSGGTAGSGITSSSDEDLPIGCIAATAANKDGGAGAVKPSAGAAAATSLVPYPLKPGGTSAAPQIRFCDEGTNQREPKTASPVTRQLPAKRKVLGLKSDPAVGRGLNPEDRNVDLAEDLAEDCVAHNGPSYPESVIAVSKAAAEAAEAAEAADLDMQRRLQMLMTVPAVEAATMVAATVITAADISSHWLPEITAGAAAVPVTAPAIGGSGATAGCDRVQPPPTEHSIQGSGAVADGCGCDKGEALAWRRLSAAPLMANQPPSAKLPAAQWFIPSSDTAAVVAAAAIHGQSTPDLFAEIRQMSAWTPSELMAAAALPGGNSYGSDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI38</accession>
    <name>A0ABQ5SI38_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013417</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044515">
        <property type="entry name" value="ABTB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46231">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB/POZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46231:SF1">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB_POZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="404"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="545"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="581"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="761"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="820"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="617"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="659"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="672"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="706"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="717"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="772"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="825"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="839" mass="85873" checksum="ACA0755877A26E58" modified="2025-10-08" version="1">MEQITLSAERIISVFTRKLPGVGQQPLVCTEQGIQLLLGFDEVNLQGACTSIRLGPPLPLTTRSPEGGTAPYTWSTASGDSDGASRMPLYVVSDAGDVGNGSLCFLSTRDAIWRLDPTGLMELVAGQPGCPGHHDGPGGSAQLRSSLIMSPDGTGGLFFSDGNREYVRRARLPALSHPSTSGMTATAIAPTSLPPALAVAVATMPFRIPEWSLVHALTYDYVANVLYVCTPSTIYHAVGVGAAAGTVAPGLQAVVGSEEGPGCLDGQGRTARFGSICDAVVDSRGCLYVIDMEGPNGGAVVRKVDPAAGWAVKTLPLACLGNLDRAARLTILPGGWLCVYEWGGSKMWLMQMGLQPSQPVPPAAALLQPAAPPAEVFGAASAGPGPADLAEDFAAMLPDPWHIADITIMVGGRAFHAHSQVLIARSEYFRRRLLQKGQSGIAAAVVGTGNDGDASAPGGAPAAVPAPICAAPDAAGRRVVPLPDADPEAFSQLLRYIYTGNMEFPKPLLRLVLELANRLLLPQVVASVQQQLKLSQQHLPAQLPPLPAAAVPSASTAPPDTAGGAAPPGLAGPCIVGGGGLPAGSPGATPSPVPGPHAAADFPPQPAATPSAPRPAQVQTAPLSSGQVPSYGPTPTVPMTITPAPLPPTAEKDSDTTPSAPPADYGPPGARPQPQQSQGPQTIQPVQPQIMHSSQPQPQPQPQPQSYPSYPYAHHPGPAPPPPAFTPPPGAYNLPPQGYAVQLAGGPSQLYGATLPSATFGAPPTASGYSPAQPPPLPYGTQGPPYPRPLGMYGAAPPPSGFGALPLPYQPQGYPAPMYGGTPLSLPPPQGGGYFPPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIJ2</accession>
    <name>A0ABQ5SIJ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003882">
        <property type="entry name" value="Pistil_extensin"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01218">
        <property type="entry name" value="PSTLEXTENSIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1194"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1205"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1292"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1359"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1391"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1423"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1480"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1526"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1566"/>
            <end position="1580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1793" mass="184598" checksum="CE343CDC4790F1B8" modified="2025-10-08" version="1">MLQPQPDSCQKVGARRCAAPGMLNDHVSGALGNADLHPRELGPFHAWSTRASLAAQYYGWSIPSQAVIAHALSASYTCTSLHHSHSNVRCKVSSISAPWRPRCRPQLPKTRCSLFLLWIPVVLLLSAWNQGAADAQMQPSPGSGNPILRPPPPNSNSFRCVMKPLTTGPAQAEAAAVVPVVGVNGSFPSGFSSPSSCAHFPLSSDESDCSCESSPYSLVWGSDVQRGQRQFHTFRLYALPGSSASATSTSRGDLSWLTIQLDPSRAGSCYKVNSSSIVQPGTLGQVLKAGAWFTPDFSFSGNYPVGLPVSGMIGYSLATTASSAFLELSLNMMDLRKRLGAATAAVPSSDVPPDPLTMGEFTLAKCGAWPGESLCASRVSSSKAASKCLVAFVWPGVLQTQLPPQPSLPSQSTPAENVTAISPLVVCFSLREKDLSPPPPSLPSVGPKPPQEPPQRLSPWPPPSLKQPSGPLGNISVSPPPSPTKAYVQQPWSSAPEPQSLLKLRSPNPDRSTPPPTSQSPPPRPSLSPPPSLLLPPSPPLSPMSPLLLPPSSPMPLPSLPTPPSPSPPSPPPSPPPPPPPPSPPPPPSPPPPPSPPSPPSPPSPSPPLSPPLPPSPPSPPSPPSPPSPPSPPPPPPPPAPSPPVPPLPRPPPSPPRPRLPVRRPPPPSPPSPPSPSSPPPPSPPPPPSRPPPSPPPPSPPPPSPPSPPPPPSPPPPSPLPPSPSPPLPPPPSPPPPSPSPPSPPPPSPPPPSPPPSPLPPSPLPPPPPPPSPLPPSPSPPSPPPPLPPSPSPLPPSPSPPPPPPSPSPPSPLPPSPSPPPPPPSPPPPSPLPPSPSPPPPPPSPPPPSPSPPSPLPPSPLPPSPSPPSPLPPSPLPPPPPPPSPLPPSPSPPSPPPPSPPPSPLPPSPLPPPPPPPSPLPPSPPPPLSPPPLPPSPLPPPPSPPSPPPPSPPPPPPSPPPPSRRPPSPPPPLSPPPPPPSPFPPSPPPPSPFPPSPLPPSPLPPSPSPPPSPPPPSPSPPPPSPSPPSPLPPSPLPPSPMPPSRRPPSPPPPLSPPPLPPSPLPPPPSPPSPPPPSPMPPSRRPPSPPPPPPPPSPLPPSPPPPSPLPPSPPPPSPMPPSPPPPLSPPPLPPSPLPPPPSPPSPPPPSPLPPSPPPPSPLPPSPPPPSPSPLPPPPSPLPPSPPPPSSPPRLSPSSSSPSSSPSSPPPPPPPPSPPLPSPLPPTSPSPPPPLPPSPSPPPPLSPSPRSSSPPPLPSLMPASMPPSPQPRLLPQLPPPRPHPPTPSSSLSFPPSPVPRPPSPTPPPPLPPSTQPLLSLPPPAPARSGSPPPLPQPVTGALVAPPLPPPTPAFLSTDPPSARPPSPTPSPQPSPPATPPVTGLRPPIPQPSPELSRPSPSLPQLLPSPPPQPERSPSLQQPASAPIPEPLSQPVPPPYPPAEAPSPLAPQPLPPKPRRPPRRPPSLPLTPAAPHIPKPRAPKPPSPPPRPRLPPPPVPQPPSPRPPSPKPRPPPVKLPRPPSPQAPLPQRFLSPKPTSSSFPPPPVHLWSEDDSPPMMPWSFGGPAPFSPDEPYAPDPPAADIPLGPDGNWTLGCRVDDDGVIHVDNDKFPFGLFHSGVCSCEAAPYFLRFGPDITYRGLTYYTFRLYPKPCNESIIIDGEPVCSTIRNLLNKIAFRLEASSTVCFDNVTTTIDKAANWRGVRFWNDSSPEQPGTWIQARAGYTTSRRHIELYIYGLRGDYVSGTFMMLKCNAMGSFYNICKRSFAGMCLYSFVDTPGHRFVTVCKTQGLPEPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIR7</accession>
    <name>A0ABQ5SIR7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013823</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DONSON family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024861">
        <property type="entry name" value="Donson"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12972">
        <property type="entry name" value="DOWNSTREAM NEIGHBOR OF SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12972:SF0">
        <property type="entry name" value="PROTEIN DOWNSTREAM NEIGHBOR OF SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="68924" checksum="A8E8A6163022ADC7" modified="2025-10-08" version="1">MTHYSSLVQPHMQTEAASRDAGCPGPATKRPRVEEVLQQHAPREQLLVSELMGWRGSSQESLSTMGTAAASIGLTDFRTLYARQPSQQHLAQQRAPNAATTGLRTFTDVASRSAMTPDDVGRQELLGALRAAYNTAGPSTCSQLGRSGAAQHALPRPQDATGMDSATGLCNPGATAASGANALEDLTLEQLPMDWGIKRSLTFTSFTRFGIHDRAQQAPPRTRWRALQNIVNSDLDTIGDEVLQYLSSQLSWRHPEVLMDASAFAAARSASAASLIGAGGTALANATPGSIGSGSGSWLAQRLVAWRSALHSLYGTYRGGDCNVFYVINSAARNPFVALFAYKGVRGSSELCAVVSQSTRVLRGRMLAAGVPFSMPLEKAAGSIDAAAASRAAGGATGNAGYEAGPAAAGQDGHPDEDVDAVLCDKGPQASPAQTGAQLLGSSVADNSRGSLLLLRGPRAVHGLYDFLMNDTALPELGGRLNGSGAGPGDEFNDLPLLLAPVPFDGGAVCRPTIKALTLNGKLAGGSVAKLGLPTTQPQAGASSESESGTVLHVLQLSGLLAPWTVRRFCSLMARLQPSGYNATLEAEPCSITLNSRVIEAHPGSDGSDSGTAAQVVRPTFAQGGALTLEELVAWQQPVVLPGGRPVKVVMCNDVRRMSVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIT8</accession>
    <name>A0ABQ5SIT8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF37">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE FTSH 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="491"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="989"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1165"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="989"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1003"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1165"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1185"/>
            <end position="1198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1216" mass="134031" checksum="8DEA9CFB2E10AAD0" modified="2025-10-08" version="1">MQAAGTYRPPCSAPSATSTSYSVQTRRNCHNYGPPGAWQGRSHRGRDFPLWSYESSCSHPTRWAVRRSAAEGQHELGPEDNFKGIPKRQLAAMALSPLAALSLPLLNDPVWQQSFTTYGERIRSVLGGPQAAQLAQQVDEHATFMDWTYGLTGVDLSAVWDPELWIRFREAVARNEPAIFWNKLIDRIQYREVLPQAGLVGDMRISYAKFLELLRDQRVKRLVIYGDMRTAVVEVPHPWSASVLGHPSTHPFYEDNDHMRVSLLTPNPAAPEDVTQWFCAEMPEWDMEKYRFYVDLPGDFWESGVLQAHLAATRTTGAVWDPVSRQYVLPYKAQKKVFQVTTEVQLLDPQESWDFMSWLLHPGRLEFYEKAACVAIALRLLGIVIALSTGSQLFQLFRVSWGKLRGEGKGKGKAKDPKKMSKQEKKESQWERLTSSRAREFMTKDEKSGKMRDTGVRFEDIAGMDYLVTEMREVVRMLKGDPAYTRVGARCPKGIIFQGPPGTGKTYLARAIAGEAGVPFFSSVGSEFVEMFAGVAAARVNNLFYNARKKSPSIIFIDEIDAIGRARSTLGADPGSMERESALLAMLVQMDGISSKTEQVLTIGATNLAQELDAALLRPGRFEVVYEVPQPGPSARMAILRYHSRNKPLDGDGNQLLLKVAEATQGWSAAALANLMNEAAILTVRRNVPTISLPMVLELVEGINWGETAPRIPESVAKDRLALVTAAKAVAFALTPGLEPIKAVTLWSRRRGLGPSVMFVDMNDKADLGLHAEETELLEYRTHHKTNAAPLGGQALGEFLHVAGLLIPLYAPRAAEVALYGKDAASLATAQSLADCFEIAYYCVRNSQVHPRFKSMPPLHTTMWMGRDEDGRWRRDPLAIGFDEELGYHKLTLTLLKASWRRALRLVASRRTAITKVAAEMLAAEDERISGERLVEIIESTPLDALDGEGLDAKAAAVVVEEAGNEFLPLLKEVLGEVPNIIMTGEKLVEDEARERQEKAKTESSNSSPQTSFHVPGAGSMGMVSFRGAPSTASSLSGAAAGPPPLLLDDETLIAVSRAVIGRLDVVDLVGRNTAMEAAERVRDALLHPQTRERLKAVRRWAEDPDGEFPPPPVTEREAEAMGPDGPLYGRLGLNLEWWSNRRSDAISWSAMEMLFNERQRKYFAEDADLPRDEDTSEGRKKKAAAASAKAQAAGAAATPPSGGGSTRAVDPPPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ20</accession>
    <name>A0ABQ5SJ20_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="764" mass="82995" checksum="1F35D7E6837E4C9A" modified="2025-10-08" version="1">MKILPSAVTRWFHGKGYLSWDPKKGEPPQYVMDDMRRVLKSANSADRERVLRHIAHLAGQGRRMQSALGRGHVTGDVCVCLQSGRSTVQIAAAEALSTLCRRHAENCDIVIATFRSSFLWFRRCLLDYMLVLMCARQNDLAIAAMGLLCELFAEKTSELLDQLKHSTYIIGKLVKMVQGWGKQARNTTPSAAANCELARGMLGFFNTMATAGGTKMCRSLDKCGVTALCLQLVEECPQQQSLVRGAMALLSVMVLQVPAQRLEFEKNQGVQRLLELLNQGILDMSVRGMAAEVLSSAASSSPKVADTIRLQGLGTVTDLLAFYLHEWLVQGVQDPCSQSAEMESSVHAPQLPSCNTEILEPLLHLLATVATSSTGEMPLSDQGLKLLGVAVRAGLAHAAGEGYKVATAAAQVALHLSCKADVPLSPALCGDEVTGPMSEMVDLACRHAAAGEVKDARILHGGLCAAGFLSKVLGARLRSNDVQLAEVLNSDLLIKLVTLLGRSADAVLVAVNAMEPCYTEPITPEGLGSGLRSTGHTYLVTRKVVADLRAAVPLQLQLLMNLAAGPFESLQVLVHLGAAAAVKQLMRWEDMKSKAAHILRLLDELDLEYKPFGDWGDGPLTAAALSSLQLDPKPFVTRGVSSSKLLKLREHDLQTRFGLDQVGIGKVALLQDGHKMFTEIEAMDKKSSGGSISMGNMEQYLVAKLNMREANAKTFSMEVFEEMQVGQMAPASFLNFIKVYPILRERLDDLMSGQRDRKRARKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL13</accession>
    <name>A0ABQ5SL13_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000092">
        <property type="protein sequence ID" value="GLI70190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="147" mass="15720" checksum="D4B52AAF7CD8C93C" modified="2025-10-08" version="1" fragment="single">DLISQLEKLRSGAAMMEARKGGLSGASTDLQGSSITAKFAIIPAASCPAMGATSPPSAVEVADAGAASIFINKAAQQPQELYQPLELTQPPKPHPKTPERRLPPEARNWGLQDCRSWAGEQHWSSWAEVEAFTKTLEPTLAEAIREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLC1</accession>
    <name>A0ABQ5SLC1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010903">
        <property type="entry name" value="DUF1517"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053023">
        <property type="entry name" value="FLAP_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33975">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTE BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33975:SF2">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTE BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07466">
        <property type="entry name" value="DUF1517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="402" mass="43096" checksum="3533A3E994280711" modified="2025-10-08" version="1">MMHGCLASLPLRSRDATAPTMAHAQQSTVVPTVAKRTDTSMSSLLKASVHIRNMAFTALASVALVVAPCDHAEAARTGGRLAGRRTAVVTRKRTALGHTGSPPRSARYRVPISVRLQALHPILSAPDLATNVDLARPPHLFGDIVAYPDLAAGIDLEALPLWASLLGLFAANIAGLALTIGVLRNLQDSGEPITVVKLQVAMLERRPQLQSKLRELNSMIQVGQQGAWLILEEAILQLIQHQGTIAYASVTQEQLSSKKRAYAVFGEIAEAEAAKASREEAVVRHMGETTDEDDSVGSAAGGGLVGGLIGSLRLGHGKPVKELTVITVVLAARGKLDIPSQVSDWPSLRHALQQVTGLSSDRVMAIELLWTPRNETDYLTREQLERDYPGLVHFGNVEVSHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLG5</accession>
    <name>A0ABQ5SLG5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">4-hydroxyphenylpyruvate dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_015381</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the 4HPPD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08342">
        <property type="entry name" value="HPPD_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041736">
        <property type="entry name" value="4OHPhenylPyrv_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01263">
        <property type="entry name" value="4HPPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959:SF1">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009283">
        <property type="entry name" value="HPP_dOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="VOC" evidence="11">
        <location>
            <begin position="38"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="11">
        <location>
            <begin position="210"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI70471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="433" mass="47509" checksum="5505FC3046C83E2A" modified="2025-10-08" version="1">MGFSRASTTGEREGGVKLVGYNNFVRHNPRSDKFAVHKFHHIEFWCADATNTFKRFQHGLGMTLVAKSDQSTSNTLFASYVLQSNDLVFAFTAPYSRKCAALSDGTPLKHYRTDEAYDFINNHGLGVKAVGLVVEDARVAYEVSVANGGKGVLSPVELTDAATGTTQVIAEVLLYGDVVLRYVSGSFTGPFLAGYVSIPGSPAISYGLQRVDHAVGNSTDLIKTVEYIIGFTGFHEFAEFVAEDVGTVDSGLNSMVLACNNEAVLLPVNEPTFGTPRKSQIQTYLEQNEGPGLQHLALLTNDIFTTLREMRARSEVGGFEFMSRPSDRYYQDLPKKIGDKLTPEQYKEVEELGILVDRDDQGVLLQIFTKPLGDRPTVFIEIIQRLGCMREVKEPETGAVVGEEQAAGCGGFGKGNFSELFKSIEDYERTLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLY3</accession>
    <name>A0ABQ5SLY3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48148">
        <property type="entry name" value="KERATINOCYTE PROLINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48148:SF2">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1497"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1200"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1570"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1801"/>
            <end position="1828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1964"/>
            <end position="1984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2055"/>
            <end position="2098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2142"/>
            <end position="2265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2282"/>
            <end position="2358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2403"/>
            <end position="2436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1217"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1238"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1582"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1818"/>
            <end position="1828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1964"/>
            <end position="1977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2162"/>
            <end position="2186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2252"/>
            <end position="2265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2295"/>
            <end position="2304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2310"/>
            <end position="2334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2343"/>
            <end position="2358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2436" mass="248323" checksum="1D5036845E7D05C8" modified="2025-10-08" version="1">MERKWGVNVLDVTSDMKTLRGGSMPLLHWLCLAVYTHILLSASFSNARSEPSKLRISKPLLQQLPDDGYGNLDENSYAENNDYPLPQAEDLSMHLRSVNPSGTGAPRPPVAETTADYIARPWLDRVPVTGAIAESMTHFGPYRSSQLADRPYHTAQVGSGAATGVDPSSGTFAAIDQTSHAASFTDTADGISGVSDGTKRTKHRDAEMKPGSVVGVLGPAIAAAGGYLPLPYRRQRRLGTDRTSYCMTAPSGMDCYPCPTYDDPCLTDDLFYLDDTKCSMPYYFFQGNVDDESICGDEIRPTNVGQMDLDTTINYGRNGSVVSNVGQMLVFMQINGLLTMTVRLSCPWLAVIDPSRRGAALQMSLTAPNGTKWTRWINVTETGSQFVSCASFSVQVKQLDPNIPGDNGCDARPYVATVAFLTETYMGYSSVGDDPATCSVNDVATTGTGVFYSLVYTPIQFRPPSPQPFPPLLPPPPPSPPRPFVPFAPDVPPDPPPATPPQSPPPPSLPPPPSPAPPLLPPAPSPSPLLPPLPLVPPAFPVPPSPPSPPPPPIPPSPAPLPPAPPPLMPPPPAPPSPLPPRPSPPLPSPPPPSPKPPVPPSLPPPSPPLPPSPVPPSPLPPSSPPTPPSPPRPPRPPPGPPPSPQPPTPPLPPVPLPPSPQPPPSPPPPSPPPPAYPPMVPFPPSPSPLPPSPDPSPPPPPPPSPPSPAPPYPSPPSPPPPPSPYPPSPQPPSPSPPPSPFPPSPQPPSPPPSPPSPPMPPSPYPPSPSPPLPPWPPSPDPPSPPPPAPPPSPPNPRPPKPPSPRPPRPPPPPRPPPPSPRPPPSPPPSPPSPPPIPPSPDPPSPDPPLPFPPKPPLLPSPPQPPSPLPPTPLPPSPPPPSPPSPPRPPAPPPSPFPLFPPRKPRRPNLPPRPPPPPRIRYPDPPSPPPGPPSPPAPPPEPPSPPPRAPYMPRRPWPPPRPPRPPPKPPRPPGGAPSAPPPPPPPPPPPLDAEPRAPIQSPSPPPAPLSPPVPPPSPPSPPFPLLVQPGCLHVSAGNGKMYGDGVLLDYGNLTLGQYDSHTLRLDISLDRYFAPGDSTIGLDLLLQPAELPLPKGCPAPSDLGYPGAGGFRTSTACDSRMIQLLVDVSGLFPCFLSSGSVLSENFLMWLAVNTSSRACSNETSDTATVWGGTTSTRLVSAGCAYVPLTLTCDNSCALPPPLQQTPDPGDVQISQGSPPPPGFPPPAVQPPRNLLPATIPPPPPPVLRPPPATSQPEPLPPPASGGLPVLVAEAVVTGGTLSTNSSLPSVEGVVIQLNYNYITATARFKGDSPYASCDTGVQSSLTSVLSGLLNFPAGNISTACTMESGSSSTAARRRRHLSSQSSAESNTTVCTWKVRTDITFKMDPAIPVEGFRTSVYRALSNGGVDGICPLGLPQGSWVVTGTVVRGISSTGGPYSDCRSLSGAVTSALQGAGANVTVTGCTAGQYYGVIPGGAAPPPPGRAAAAAAAKAGPPMAMVAAAAGGAAALGALAAAAIWYYRRTKQKISFDVASEAEPGKADDNAATAIAAGANEGTEGARLWAQTITSPREAGGDAGAGSDGRPSPSALSGASTAATAAAGGGIAGKAMSQARGKTASVIALPDPDPDFSGDSIASLTLPAGEERELAAAAAAGAGGGGGAVALAAMAAHPLAKPLPPLPPIRTAMAKLPELDPGIARRKSFTNKPLSSVTAAPHARKSALPALAVPTAALATAGPNPLSKPVLPTLKGRDASSSADAAAAIAAAAPLPGVMPSATVGMAAMTEYDKELAAAHKMSAESAVLESSPAEGLSPASGANPPSQGRVSNTGLYVRMGSAGAAAGAATLKVPTLPDMPGEAMEAAGAAAAAGAGTSAMGAAVGAGGGGKRRRVVSWDGIGAGAGASSKSLFTRFFGSMFGGTAAGGGDIHPQHAVQELPTLSQLDEEAATASAAAAAAAGGAVTARRGSRFSGSNTSHSGSGSGGAPFSATQIGIVLSPVAGGERTSHGPRADALLSQTAGWDMATGATGTAATMATTSGGGGGKVSSLPPLFLKQNPAFKLPRDDDSPVVSEGDADGTGGNGTIRFKRPGEGMHGGLDDDGAAAAAADGASLAAAGHRTVHFNRPGDASNGGLHEDAAAAAAAAAGSGAPGANGSRTVRFSRPGNNFNGGLDDNGDATAAGAAAKLASSGGGRSVPHFSRPDGRDTSGMDSPAEAAQDIPQLDDKRRRSDLPPNLRDFQRPDMGDHNGSMDPEAAAAAAAGPTSAAVATAMHRHWQIKFAKNDAGNAPEGEEEDVQGSLQEGTQGQSAGGSHPHDNRNVRQFKFAHDMRSRSMPRDEFDEDVLPDEEKKEGLPEGASEEERRMYAMHHAARGPMYGDEKIGPVDQIELQREMRRRALLEQMKNSAWTGTFAPDPSPDASGNGSGIERVIGPKSPLGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM46</accession>
    <name>A0ABQ5SM46_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="228" mass="24479" checksum="A896A58A69161A8F" modified="2025-10-08" version="1" fragment="single">QVSDLISQLEKLRSGAAMMEARKGGLSGASTDLQGSSITAKFAIIPAASCPAMGATSPPSAVEVADAGAASIFINKAAQQPQELYQPLELTQPPKPHPKTPERRLPPEARNWGLQDCRSWAGEQHWSSWAEVQAFTKTLEPTLAEAIREASLEHNPLVAFGKEQAAAPAISYVQLKLMELCGSNRWGTASIKADSLEGPRKRRPFLAAFLRAAKGITEPCVQRGGIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SML1</accession>
    <name>A0ABQ5SML1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPIase cyclophilin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044233">
        <property type="entry name" value="CYP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47511">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47511:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045867156" description="PPIase cyclophilin-type domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="2">
        <location>
            <begin position="40"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="246" mass="27855" checksum="8AF073E682C0E092" modified="2025-10-08" version="1">MDWQVYHKKRMPFLTIIFSLFAIIGTVAGDDEFTPAMADERVVFQTEYGDITFGFWPEVAPVTSAHIFKLARMGCYTSNHFFRVDKGFVAQISDVLGGRTIPMNDEQQNEASKTVPLEVVQGVKHHAGVLSMGRYDDPNSGASSFSVLLGDAPHLDMQYTAFAKVTEGMETLRKMEDLPTKREGIFVMPLKRVTILATYWYRINHPEPSLTMPDSHKCEDDLAELVLRFTSQAEELQRVRKKCLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMU3</accession>
    <name>A0ABQ5SMU3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015780</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="134" mass="14787" checksum="EDC732D728DAA94F" modified="2025-10-08" version="1">MTRSGLRKAGMRRDSQHAASGIGADEHLARSWRRVASTHALQLLIAPENAKAGKRSLTSKLKGLLMLIAALTLVMMHVAHAAATWLPPSSLPPFLLAIRKHSQLLFHIEFTLHGTAIAAFLHWFSLKIFKHNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMU4</accession>
    <name>A0ABQ5SMU4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CEMIP beta-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055401">
        <property type="entry name" value="CEMIP_beta-hel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052334">
        <property type="entry name" value="G8_domain-comF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47687:SF4">
        <property type="entry name" value="G8 DOMAIN-CONTAINING PROTEIN DDB_G0286311-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47687">
        <property type="entry name" value="G8 DOMAIN-CONTAINING PROTEIN DDB_G0288475-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24606">
        <property type="entry name" value="CEMIP_beta-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CEMIP beta-helix" evidence="1">
        <location>
            <begin position="15"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="13707" checksum="6C4268552695D3D3" modified="2025-10-08" version="1" fragment="single">DFCAGQGPDLAHPADAAAGVFYAANPNNAFKNNAASGGFAGFNFPVLPEPIGDHRWMRATLNPSKRPLLLFDGNTAHSSGYFWIMAGGIYVGGKLWINDADSGKLYYSNGRYEMDTRDDTGTSLVFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNC5</accession>
    <name>A0ABQ5SNC5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GPI-anchored surface protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="522" mass="55325" checksum="BEF319AB44D666D6" modified="2025-10-08" version="1">MSLATSRAVELLGRNAEKIPVHKHLSYPHNYAQPSVAAADAGSCDDSHQKDVNPHARAVLSCTETAETEHQNSESLPVIAEAEPEFFAPPARAPEAGRSMCPSPAFNLDVCPSFSEQRPQGTKPLFIERHNTLQRVHENTLTDGALVAPRMLDTGHPIGPAGGAPYLPSWEISRSQLWMMTAASIGKIDTLPPTGSATTTTTTTTTTTLTTAQGSAIGLDRSSSVRDASTATSTPFACRFTQASVTQQVPGLSGLFRGKSMIHRECPATPHGMGPLPTLLTPSFTMASLAARPGGGKSSRDGVVGELPSLEPEWECFAAVPELPVEGTTGGSEKGEDEDPLHHHHRQKDDVAFVAGIAAATSTATVVSHAASSAMTGLAGLLSQMSMSGSTMLKHLGYNLLNVGSSLMVSRLQSMIMNTSTMKAAEAVLGAEAPEVSREQLRQRLRYTLAGASVMWFLLNLHQTTDVLTHLDFEHDFVTSTKVVLNLATSGPGLIKNCQELLQVSFAVTLGVGKRVMGADEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNP7</accession>
    <name>A0ABQ5SNP7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F5/8 type C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016267</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051941">
        <property type="entry name" value="BG_Antigen-Binding_Lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713:SF6">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713">
        <property type="entry name" value="FTP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22633">
        <property type="entry name" value="F5_F8_type_C_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="779"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="945"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="1350"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="1577"/>
            <end position="1718"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="3">
        <location>
            <begin position="1734"/>
            <end position="1821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="908"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1106"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1297"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1467"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1839"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="732"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="911"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1106"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1299"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1470"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1845"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1863" mass="203576" checksum="0F168689E87AFE01" modified="2025-10-08" version="1" fragment="single">FLYASCNSYQGTCIVLLKGIDMASLRHLGLLLLLCSYRIFLKTEAIDLSHYIRSRFLGPGALKSPKPDPSCDTSPARSLVCQVESTFLRLLPLSTGALVEVANIVRSYPAAADWFFSRWKDGSLAGIVTEAFEDIGSPASKEVLHPMLRVFLQTETGSVITDKVLDSATQAGGAIDQLLVLGQFLLSYLRHWGAHASDPSREVLYAISEELVNEEGRLTVREVVLDSLSVARTFVKSVLDAVRLDGSGLQEALKLSKADRRALESFLDSLLKLLDLAEDAVNAFYDDENIPPPPRPPKPPSPPSPPRPPSPAPRPPSPPRPPNPPPSPPSPPQPPKPPSPAPQPPRPPLPPSPRPPPPPSPPRPPFPSPPPPEPPSPPPSPPLPPDSANIALYSWAYSSSSVDTYWSSTSNYAIDGDKSTIMDSWPDFKYRWLSINLFSAYDISQIVIWKRQDCCLEYLAFTELRLGFQGIYSISDTDAIRNNRLVWSQNGTGRGDTPTSPYIIRLNPPVMASWITLQSFNPSTRLTLQVADVQVYGTYSTKSWPLSPPASPPPSPPRPPPSRPPPPPKPHVNDNLALDKPAYSSGRYTPAVRAVDGDISTAMDTWPDMVGRWLSIDLLAEYDISRIVFWNNDDCCWMELDGAEIRIGHRSIVAWADTPYLSENPLVRTLHRDDVNYYSEDPWSKYVVLELDPPVMGRWITVQSFSPFTYISLRVAEMEVYGILSTSPMPAPVPPSPPVPRPPPVPPAPGPPKPPSSYGGNLALGKTAYSSGGYYPASWAVDGNISTIASTYPDSYYRWLSIDLGARYDISRIVLWNDDTGSCCYNELLYAEIRVGPYSITSQDEVYSSINKSTLVWTPTSSSIGYGAGRSYTMDLNPPVMGRWITIKGAGYYTSSYLRIVEIEVYGKPTPSSPPPPPSAPSPLLPPSPPVPGPSRPAPSPPMPRPPPRPPGAFGGNLAMGKTAYSSGGYYPASWAVDGDISTIASSYPDSYYRWLSIDLGARYDISRIVLWNDDTGSCCYYELLYAEIRVGPYTITSQDEVYNSMSKNTLVWTPTSSSIGYDTGRSYTMDLNPPVMGRWITIKGAGYYTSYVKVAEIEIFGTLAPPSPPSPPSPPVPSPSPRPPSPPTPRPPPYPPGTHGGNLAVGKTAYSSGGYYPASWAVDGNISTIASTYPDSYYRWLSIDLGARYDISRIVLWNDDTGICCYNELLYAEIRVGPYSITSQDEAYNSMNKNTLVWTPSFSSIGYGARESYIMDLNPSVMGRWITIKGSGYYTSYLKIAEIEVFGKLGGSVTPSSLPQPPMPLPPRPPRPPRPPSLSGNLALRKPTYSSGGEYPVSQAVDGDISTTASTWPDTSSKWISIDLRANCSVSRIVLWNNEDCCWADYKDSEIRLGFRSITALDDTSYISYNQRVWSSWDEPNYDTTPWAKYVEVILNPPVMSRWVIVQSSNFGDTPLRIAEIEVYGTIEPSPPLPPRPPLPPSPPRPMIPPPPPMPPRPPRPPLSPPPRPPLPPTPSPRPPRPPSPPLPPSPRPRAPSPPFPPSPRPSPPSPPVPLSPPPRPPSPPPLPPPSSRPPPSPPSPALVNIALSKPAYPASSNFWKAATYAVDGNYTTLMDTWSEKAYRWLSVDLGAIWDISRFVIVKRQNYYSSYSTVDEVRVGYRNISVLNDTWAIQDNPLVKSITSNTTAAEVIADGAYNYSKYTVTLDPPVRGRWITVEGQLNKCDQQVSTGPVCGALSATLSCPSGLYILSVISAFYGRSDNTTCPLGGAMRNTSCSAPGVSDIVKYNCLGTNTCTVSMSSLEDPCFGTYKWINITACCSTGAYLQVAELEVYGTSNTSQLPISPSPQPTKPTPPRPRPSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNX5</accession>
    <name>A0ABQ5SNX5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_016210</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="7">
        <location>
            <begin position="4"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase zinc-binding" evidence="8">
        <location>
            <begin position="170"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI71128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="27596" checksum="1A1398775910B9A3" modified="2025-10-08" version="1" fragment="single">LQEGRPIAFTSKKFSGAEICYTTGEQELLAVLHALKEWRCYLEGRPFTLKTDHKPLTFLQSVPTLNRRQARWMEYLARFNCTWEYISGKLNIADALSRHPLLHAAILAAPVVRPRSEEVLIATDLAERLKVAYNADAWFADPANVENLRKQNGLWLRTEGAPTQIVVPNDDALRYDILARFHKDPLAGHPGSKRLIDLVQRSFWWPRLAKDAANFVQTCSLCQRNKALSDPSPLRPPPPPPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP26</accession>
    <name>A0ABQ5SP26_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="168" mass="16919" checksum="6353812C823ECDA7" modified="2025-10-08" version="1" fragment="single">IIATNSSDNGADGGSGGGSGGSGRGNSGGSDRDVSGGDSDRLSGAWVLVSNTTRESHPGCTYGNIITNMQRVGALGVIFSASPGNEVEVIKPTEVDFQQDGLYGATTAYGKYNRQRQDQHLPAPLRRCRAAASTSIVGGSHDAAGVTLPMGRTSGGSGTAAAEGEENM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP33</accession>
    <name>A0ABQ5SP33_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_017077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="148" mass="15903" checksum="B5580BAD4C6FEBE6" modified="2025-10-08" version="1">MSAEHDDTPHTSLASQPDFVVAARAPDRGLETVGVRATQEPLFLQTPAHLIVPHSAVNTNMDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTDKTPGHSIADATRAYVRDAKKYLQAVHGGDNDGIRCLFVGNTLEGSAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQU7</accession>
    <name>A0ABQ5SQU7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Limiting CO2-inducible protein B/C beta carbonyic anhydrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_017071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040703">
        <property type="entry name" value="LCIB/C_CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38016:SF1">
        <property type="entry name" value="LIMITING CO2-INDUCIBLE PROTEIN B_C BETA CARBONYIC ANHYDRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38016">
        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18599">
        <property type="entry name" value="LCIB_C_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Limiting CO2-inducible protein B/C beta carbonyic anhydrase" evidence="2">
        <location>
            <begin position="28"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="381" mass="40996" checksum="BAA20964E9AF6CDF" modified="2025-10-08" version="1" fragment="single">PATAWATHERAPGLTERLAEVTKHFPTALSVDDFISRVEIALAGYGFTGDNSIAMSNLCRDESCLILEDKIESAFGSCFSTHGLGGVLTCGVIGMKAGLSHSPVVGGKERYVFFSFPHIAIDSEGKVGAVSRPNRPGASAACGALIACLGDLKRDGLEAHVRAPGVHDPLEPEYSILKQRIARRLAYEGVKPQEMDLVEITKAAERVISTDLEYLISKAVDPHKANYAVFTGVQIHNWAADLNNTDVPSLEFVGVGRSYVVFNGEKIHLDLNKVPALSPRQLQILATASTADGKAATAAAAGKLVQEIPRDYLLKRLGGAQSRSHVDSGYEPSWGSYVTTQQREDPHVGAPQMDHPFEAADAANQEGRTSTNTSFFWRGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLA9</accession>
    <name>A0ABQ5RLA9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36792">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36792:SF5">
        <property type="entry name" value="SEL1 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="120" mass="13465" checksum="9C87B258ABA891FE" modified="2025-10-08" version="1" fragment="single">MHQSSLQSVPQTRASVKGVPTRPMSSSSYPSGRPKSSNKRSVAELERPGSAQAAPFQQQMQPEPIPLKYVVQEAVKRWFEDTLLEAQRGDVKQQALLGEMYKEGYGCQKDVRAGKEWSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLH5</accession>
    <name>A0ABQ5RLH5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Raptor N-terminal CASPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_000217</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat RAPTOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029347">
        <property type="entry name" value="Raptor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14538">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01302">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Raptor N-terminal CASPase-like" evidence="6">
        <location>
            <begin position="83"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1215"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="677"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="773"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="900"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="773"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="792"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="917"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="952"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="968"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI58486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1553" mass="165203" checksum="DB8D92D89831DFF1" modified="2025-10-08" version="1">MDEGLGEDAAAVNDSLASSLADELSRRLTNSDPVPPPPQTENGEFTKTPPRIFCEERHDATSDDADLRAVDHGLVSKWRQKEKLKTTSVALVFCLNIGVDPPDVIKISPCARLECWVDPLSAQPAKALEHIGKNLQAQYERWQPRAKYKMHLDPTVDDVKKLCLSCRRNAKNERVLFHYNGHGVPRPTANGEVWVFNSRYTQYIPLSIYELQSWLGTPCIYVLDCSAAGLIINAIRQLMDQRSNDALRGMAGMYAGGGGGAPDHLKEIIVLGACGSTELLPQNPDLPADVFTACLTTPIKVALRWFCSRSLMRHDGLTKDLIDRIPGKQTDRKTPLGELNWIFTAITDTIAWNVLPRPLFQKLFRSDLLVASLFRNFLLAERIMTAAGCTPVSYPRLPPTHQHPMWQAWDLAAEMCLVQLPTLSSDQQADFTPSTFFSEQLTAFELWLAHGSRDKKPPEQLPIVPQVLLSQVHRLRALVLLGRFLDMGPWAVELALSVGIFPYVLKLLQTTSSDLRSTLVFIWAKVLALDRSCQVDLVKDSGHQYFIRYMDSVDGLIDLYSRAQAVFVLSVICDGHPKGQALCAHNQLLGVLLRWLRSLAPVNITYGPPGHPLLLRWLCLSIGKLCEDIPEFSLHAIREGAADILVSLLTSPLPEIRAAAIFGLACLIHSCPDPSDPSHVHHSGGPGGIAGGANPVPEHAGLTLTPSEDRLPAERLIAGAVTQVVYDPAVLVRAELAVLFARFVRGHGAAVREAMAVQQRRLEEVVAQARAAGVSADDGSGGYRPRSSPGHGQDGSGAGGGTGPQEGAVTAAAQQHHGLYSRIAEAITMLALDPAPKVARYGRDVLRIAQYELSFASTGGTHFAASGSLSPRRGDSSVGSFAAKLKPRSWRSSFTIAGLPNLSRASTDPHDPHHGGSASSSYNPSTSVPGATAVGGASSGPPTAMMRRMPGAGTGTATGTGGGGSGGAAASTATSTSASPPSPGRDVPLAYSRHPYTLRIVTDQPSMGPGAGSSSVGGLSDYDQMHHGAHQGYSDTRAHGGSGLAGAMLSGGQDPSLAGALPVGQAPVAVPKSLIYGLSCDYFSRPMLEPQSSAWRESDGAKLCPWTAPVDPERKTRRRAMMDVAAAISKAMRTPKLREQLHSIESGPEPVSALTFHPYHQVLVSADVRGMLKAHTYPDNKLLNSFHVTNGAPHGESRSSLPSRVVFLHHINEADGPLLMAGSADGAVRVWRSYLNQGEQRMATALQAVSIHLVPTLSFPTVYNWSPSACHLFASGGKQPDYIYVWDLLREHCHSVLPLTGTTIGTASAPGGVVPPGVSWPAGTGGTASGGTGVEQLLLSSLDPNLLVPVCTDGTLRIFDLRAPSAPRMLLQPLGRAALAGSVLEPGGRQCMMVVAAEKGEMRWLDLRGGTGGGLGGALAASATEELATDSATTNTIIDAAHTYKTVQAHTKGGVCALAGHTLAPLLATGTTSQVVKIWTDDGDVVGTLRPGNSSNLLAPHKVGPVTAMAFHSYQPVLAAAGMDHCTVYELYDPGAADRAAVAVRAERAGSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLZ7</accession>
    <name>A0ABQ5RLZ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="3">
        <location>
            <begin position="7"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="269" mass="29961" checksum="A43DD764160C5BB2" modified="2025-10-08" version="1">MAGVHKQCMTRLQKEYKCLLKDPVPNIIAHPSPANLLEWHFVLEGAKGTEYEGGVYHGKLVFPPEYPFKPPSISLFTPNGRFATNTKLCLSMTDYHPESWNPMWSVGTILTGLLSFMYDTQATTGSITTTKQEKERMAREALAFNAKNPTFRKLFPNWVEELSRRTAAEALPVEPQTLGTVPERMQSEQATDQRQGEPPTKPGDAGALLRWQQQQPRPGHHPHQPFGNTVVTLAIVVAVLAAVWVSASSPGRDMLRSWSAWPGPDRSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM48</accession>
    <name>A0ABQ5RM48_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000288</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="642"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="225"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="391"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="656"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="688" mass="73843" checksum="BFFF07EDB9BCC8ED" modified="2025-10-08" version="1">MPLGCFGPSSGEKQLQAELDRTTAQLSSKEREIADLKRRNEEAFARQAQAQRAAAEDFERGANLLQRQVEELTKALQVEELKAAQASDKLKAALTEVGDIKRQLEEERAAARSAEHARATAAAVQAQLADYEKQIRDSHAHLEALTQELAVARKEAAAASGDASLAHEDAANAREQLVKALADQSKAITEAEERAAAVAQEAAASTVAAAAELAEKASISNRQQVEFLKAQLKAQQIRLEAAEQVEAELPALRMQLQDLQQQLAAVHTESSSSKELQRNLSERLKDFQSELEDANLIRQSLEAERQVLVEQLEQLTADRARLEAQMQDLEASLLDSVAPAVMSASSVPAAEATAGMALAQGDGTECAVPRSAGGSSEEGETGLARLEVLSTKTLAAQLLETQEEARDLRKELERTLVGHAEEEESLQMEIVVLKGQVELVRAALTAAESRIRELEVAQSLKDQLGVQLENTQHALEEARSSVEDLTQEIVGLELERAALRSREDEKQAEVTKLQQQLKEAQMRAVELVSANADLQVEQAKLRSEVLSQGLLSDSNTQMAAKLLTDYQALKHEHAKLLAEVRRKDETMALLQEQLSSLTEIGLDSMLRQIDHVQMRKPAKKSMAAAAPVEAMLAAAGLAGKSTAAAPHSSSGGGSTGTPPAVTGAAASTNGIATPTVAIAGPNIEVADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMA1</accession>
    <name>A0ABQ5RMA1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF100">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="59"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="697" mass="73896" checksum="06226C1FF690EC95" modified="2025-10-08" version="1">MTIITKMTVFDTKNWIVRPLSNVGKGLARSRMVFKPVVPAAGKTTAEKTTRSHDTINVMTYNILAQKYAQSGRHNYCPSRHLRWDYRKALLFEELEAYCSDIICLQEVEAEVFTGELQPWLAERGYRGHYLARQHGENVQGPPEGVALFYRTDVFELLQQRSFTFSSVSTDPPAPGSLWLSSSDSEDSEGTSVAASAAATLSVTTDPAAASAVPAAVVPAVPHQLPLEAGRGSPLLFSDSDAEPETREDDDRAAPRVAETERGPDTVAGTAAKIMAACGSQSQPCTSCNGSSNLAVAVSSGVLTSSPAGAVSAYISGQGGCSTSTSASSTSTSSSCSSINELNGGGGISYGDAQLGSSYAERKKDRGRVATTAASDAGTVPPEARTGVVTGNLTSLDEEPDPRSSSPLSLEDTPGAASSSSSSSSSSSSSSSSNSSSCSTSSRSKRGRGNNEFWRVFSRRREGAILALLRHRSTQRLLLAAVTHLFWNPAFPDVKSFQAAVLCQEMAAFLQRHARGGNGSGPAGDAGVVEEVPVVLSGDFNSLSHKRVPDVFDPKIPPGRDGLVSGVYTLLTRGSLPTDHPDHPASRRRLGETSNTDFRGLDLTTAGLRLASAYCLANGREPPLTTRTTTFAGCLDYIFVSPQHFDVESTLDFPYDLPLGSYDTIRDPVSDVEFPPIPNGAFPSDHLSLVALLKFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMC0</accession>
    <name>A0ABQ5RMC0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000335</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="509"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="565"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="610"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="674"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="421"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="529"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="613"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="693"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="702" mass="72984" checksum="B81098F127F19E4C" modified="2025-10-08" version="1">MLPSYHHGGSMPGMGSKPPRRPWFQDVNGSLIAILVLAVVLALEHFHFQSRLARVSALEVSLATQLKDVEASMKQHTDQLRKMNPKLDEIDNTELSLKRLQEEASKATSGLADVRATLAGIQRANITAQLVQEVQQQWQNLTAIASQVDELMSDNDTLTDLGSRLAMLQSNFTKLASKLESHNSALKTLADQLELHQNSTLSALQQQTVAVSEALEAHKALQERVDNLNMSAAAATPNAVKVGAIGGAGSPADGGVGMGNADISSITTKGVLAVREDNISNIARGREQLSGIGNGTAASFQLGRPVDGWNTTTGVAGAPKLAVGVNNTGTNQAAAEADVSSVDEENQLVREHVTLTLDDGEESSNANNKKGGPNSTTAVTASAPISATAITQASGSTTAADTVQYDNTRVWTGWAAPKITEQNGTTAGLPTDASSNKTAAGQGAAGTVTEADVKAVAQFGTTASAGVGSIEGSANMSKAVITTGTAQFTKKTAGNSTVALNELNALVSAAGSPRNEAEARKGSTGSTGGTSRTGTTTAMSSEGNYSKAVADDVWGWQSDHQVHERVSSAELPRGHSGLERDQGQRTRRKVVRTGRNQFDRMHEQFAKQFQQHHGSDDAVADADRNSTSEASVRVGAKSTFEGTGEGAASKAVDVLATVGAANRDEATSVVITGSGKGVTSSVQAAGENSGVLLDEEEQDRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMS8</accession>
    <name>A0ABQ5RMS8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000641</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47026:SF2">
        <property type="entry name" value="FLAGELLAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47026">
        <property type="entry name" value="PIGMENTOSA GTPASE REGULATOR-LIKE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="359"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="397"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="442"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="545" mass="58327" checksum="58BE792F1894035F" modified="2025-10-08" version="1">MNYWYAPKSDPQRQNASELPTELIAAIAAPWRSTKELEAIHAKARRLAHYDVAGAAQDVLVTRQQQESKGAVKALRRGQLMVQLQTDTAALEALTAVEKMWSVHLEQLRREASAAVRALQEKHEDALTKMRLEQVMKAKEMGIRPERRSKELLNLRRSESVLAANNNFHEAAKVKAHADELAAAESSNAHMAWLLKSHGEQERLLARQRKELEALRNRLDERLQDVRAARDSDMRKLEQKIKVTSSHQQRCFTRERNFLEQALKGDTIDTTTTRIGRTLADALPTLAEMAMAAAKQQPLANVEPQAAGNSARQSSVKPAESTPSTGYLGPSGTSPAGVMGRGRSVVLAAAPSVRFAPTPPREAWAAVTKSDGAGADSGSRAEQGDKSDSGTLGGGSSPQSRLTTASSSSSSSSSSSNSRGLGSREGSRSWSIKSQEHDPSRQTRLTSATMNEQLKAEPIQTAEPVKGPSSSAAVGSGTTAAATGSGSSALTEEALRAMNQATGVRLKDGHRGTSSLQSSSFKSNKPYAGGVSARTVARPISAAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN14</accession>
    <name>A0ABQ5RN14_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Component of cytosolic 80S ribosome and 60S large subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000738</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_eL31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13250" checksum="956BC13BE9F49D6B" modified="2025-10-08" version="1">MAKEKSRSKEQVTREYTIHLSKRLYKTSFKKCAPKAVKEIRKFAAKVMGTTDVRLDVKLNKAVWAKGVKNVPTRLRIAISRRRNDDEDAKEEMYSYVTVAEDQSTKGKGTVVVQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN57</accession>
    <name>A0ABQ5RN57_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="171" mass="18700" checksum="BA54989FBE1A303D" modified="2025-10-08" version="1">MDAADRAYVTPPPERLLFSVSPVLGQSALAEARHMQYPAHLPTMQSTAGQAGNQATHQVTRRVAGVGARCIAGVSSGQDSCVGWSRDLLLRQAALLPAISSVEGLKGSAQFQEYIRNTPRGLLVAKQCQDKIETYQRLRQLDPDAFRQLFSRDDIQVCDLSVLSITYLLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNH1</accession>
    <name>A0ABQ5RNH1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000796</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044978">
        <property type="entry name" value="GRV2/DNAJC13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045802">
        <property type="entry name" value="GRV2/DNAJC13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025640">
        <property type="entry name" value="GYF_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36983">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36983:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14237">
        <property type="entry name" value="GYF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19432">
        <property type="entry name" value="RME-8_N"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="1817"/>
            <end position="1880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1013"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1458"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1929"/>
            <end position="1959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2413"/>
            <end position="2470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2569"/>
            <end position="2622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2832"/>
            <end position="2854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2960"/>
            <end position="2983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1458"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1485"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1939"/>
            <end position="1953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2423"/>
            <end position="2435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2570"/>
            <end position="2580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2835"/>
            <end position="2854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2965"/>
            <end position="2975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3068" mass="316923" checksum="76141CD9D95E0DEB" modified="2025-10-08" version="1">MDLLNWHASPQQKPANCLARYVVTKHSWRGKYRRLMCITPDAIVTQNPESGMAITNTYAFSGDSDVESISIGTGNDAEGEFTINARQDKKSKFKPIKFTCSHRSRLLTDLFHSVAAAHLRGRCGVAQRLIGSPDHSPGYEWSGGQWRPTTLRVTGYGLELVDMEGTPTWRLDYCMMSSVAFAPLVPEPLPTADGIAGGSGLSTTSSYGLAPTPEGPFAVFSRCSRAPRLLACRQRDGVLHYMAATAQQRLGLHIIADNTSGSCPSALLEAVARSERQAFGGAGEAPVAEWEVARLCPRYDAQPPLAALREPGMQARCRPAECLDPTAQQAAAATAAAAVSYSPGAHTVPRRLAVTRSWLLERDATTYDVEERRPLGSVSALLRFAEEPGLLGVEWADGAAPSLYMAGAARDGVLAALLDMAQCASGRPVPVLPGFTPTGDPIVGSAAAALSCHGAIVTRDVETERWCMEQLVARARAAWPHLSNSGRMFYSADIEFSSISPAPSTLAAATLPTSPRHQQQLAKVDAAGAGHGRGHAAAPQMPLQPQTGIVLSGQLSSATASDSARTAAAVHLATQTMLDVVYDFNASIPYAGISAGTKVDEQALACVLALLHPELVPGPVPLSHLTADEAKQSIAALQALQRLVSSHTLAEMFLGLPTAVGRVMAAYACGHDGVAAEAARLLTRLWAPAAARVGAAPWSGLRAAPSLASLLADPDDPLSGCDPEDLSLARSAKALCFGPSIITSRCKALMWPLRGGCSTDSSGSSSASAAPAAAGSNPNGVVRIGRPPSLAALGASAAGGGVGGMAGALLVACALEAVGAVVVEPGCRSTDNRPLTILLQEAAELGPPLFALCHHPAPRVTQGAALLMRAVAESGAASAAPMRQAALRHGAVLHHLHAALFAAPITTAAAAAGGGSSAAAGEDGGTASGRQQLGRALVASWCDEYAPALALLRRIFPPGLVRYLNAPRTSGGSGSGSKPVALLAGATTAAVGAAAAAVPLAASYPSAAGAAEASTAPHQQLQRSSASGMSDGAAAHTSAPAPASGSNSILVSPSSPRSGALASSTATGIAPPSSRTGAATSSSWGFKGNWEAFWEAATRDHCHAGLIWHAGCRQELREVLEREEAGLRARRQQLGGGGSGRLQLGWNYEEFGVTYPSLAKHVAVGGIYVRLLLEGADTAAVEKVPQPRELFNSLHHAFLCAADPVTQGFQNAAAATAVATAAAAVSAGTSTAIVGGVTDASTAVAAAAAVADAAVTADQELSARAMAAVYHVHAGTIGPTDGLRHLVHVLDYTFCRKLRHALLELVVALLSPRCLTAASGTAGGAAGAVTSPAARAARVNSYAFMEAGGLELLTDMVAYAHTGGAAVEGGGGAAAASSATGGGAAGGAWGVTASTAAQRQQQVGAQSGLMLTSVGHEDMPKEWYFYPCGVILPAAAAPTVSPSPGAAATDDLLGEELSTPTAASASVTGAPGSGSGSGSGSDTLLGRKLDETGRAGPYTRDEVRQLVSRGSVDMATLCWATGMQRPEPLGSIRELRWMLARGPGRFSPYAAAELALQLLQRLVELQPAVDEEEGLVLQPLPRAYRVLAGPRCLPHLCQAILTFHPPIVSAICGLLDTLLTPHPDALARLHLTGVFYFVLAYPGSDLMAPARLLHRTHLMQDFAGLTGGAAAAAGLPLRQRSYLGHVLPESLLYVLVTRGSAAFAAALCGDHGNPELIWTHAMRRSRLLPALSSHLGDLPLRLAQRCGTLWDYSPLPPLSYPELEGELYCHRYYLRHLADTVRFPSWPLVDHVPLLQSLLVEWRAELARQPLSLSEAEACAVLGLRPTYQGDNAVAAISEEELRSAYRALARKYHPDKNPQGRPMFLKIQAAYERLQAGVAGGQGPQPWRIVLLLRAQCVLYSRCGKDLAPYKYAGYGLLLDTIRGGTARGSTTGEKPAAGRAATASASSAAGKANEESPESVAPGSFFAGEVLDQVTSAAELCWLTVLSCRRNAEELSRCGGVGVLAELLSRAVGVLPLDAAPTDPTAVIAIAALRSLAGLAVVPEARAAMATMLAAAGPSAPAAAGLCRDLVRCCRLTRCHAAVDAALVAISYMAAAPELQAALLRRGALQALVPLVLQYDSTLPESVRVTLAPPFAGPDDSATLPLLALPENAPARSSTPAARSLHAVLAAQALSRLSGLLPAPHSSPPCAPARAALAALLTEPLSGRLSEPDPRPLLGVLSGTHETCRVIWNPAMREELLRFLDPSAHTATDQDHELETPGQSAETRAAEAEGAGTATAMAALGAHAAGVSSGDDLLSGLCSFSHAALAGELVVGGVFVRVFNDRPAELPPDPSGFCKDLVRYLFDALLPLPPGSLPSAASLPPLVAVLAATGHLLDAEPRLLGVLASRSALSPFVSCLQPAAYQALTNPHLHTEPQPPESSEQLQSKQLEQPTEEGQAANTGPAGGPSRASWDGSADGPVGHWRPSASGGQPAAWYWANRLAVAGMALLSRAAQHSGCLEAFADEHCARLLFWLLHRPPSLEALAAALRLLRSVSGSAPCAVVAALQGGWLYLLEVLLHRGPWPWQQQQQQQQQQQGPTASPETAIAAGPTAPPTGPAAGGKDRGDGGRGAAEAAEAAAADDARGEAAALLGRLMAHATHGSLIRLSLQRLLPPGLVASIAEGPPTVVLQALTQRVETPECIWDNEMACNAATQVAVLASQLRGCQAEGQYDWALPPGAAVQYDKLRGELFVGGAYVRLFLKNPKHPLRNPSKFAEGLLERYLSELASATRDPDMLVLLAAALVALLRSHSLLAEQLCRGGYVSKLLAQLGTLTRNLQPSQPPILSSAVGATSSAADGTPPAAAGTGAGALSSPPLPRAAAAGLAEAYGSALRILHQLCDSPSVAEAFATASPPVVQVLCDTMRLGAAARVLALEALKRALAPVNRQRDALVLQALQYGLIPELLAYLDWQSSRGGPTVGPGPAANSSSAPGGDGGGGGGGDDDVAVLRVLAVDVLKALQLDGLHSQQVNNLLDGSAVWQAYCHQRHDLYLPSGATLQGSVVGLLTAPETARFALPPPEALGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP99</accession>
    <name>A0ABQ5RP99_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000963</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="10745" checksum="5BD00577CC3BD8F4" modified="2025-10-08" version="1" fragment="single">ALPPVAGGDLAWPGAEGWRVLMDEMQVRLTDMSLVVGYTNDSTAGAPGGGGGVSAYNTSYNSTGTGGGGQNIIRDVGSGVIVSLHRPLMIQPDRDAADSAVAADLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQF4</accession>
    <name>A0ABQ5RQF4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001772</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_bL12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_bL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_bL12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_bL12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_bL12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00855">
        <property type="entry name" value="L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL12M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="Ribosomal protein L7/12, oligomerisation (N-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL12 oligomerization" evidence="5">
        <location>
            <begin position="39"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein bL12 C-terminal" evidence="4">
        <location>
            <begin position="97"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="164" mass="17183" checksum="21ECAE67BC8E8DC8" modified="2025-10-08" version="1">MALCMQTRSIAGVRSRTSAVRSAPFRGSRMVVRASPAVNEIVDKLKTLTLLEASELVSEIEKTFGVDASAAAPVAFAAMPAAGAAAAAPVVEEKTTFDVVLEEIPADKKVGIYKVVRNIANIAVNQVKEFTATMPKVLKEGLSKEDAEAAKAQLVEAGAKAKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQS1</accession>
    <name>A0ABQ5RQS1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF135">
        <property type="entry name" value="CSC1_OSCA1-LIKE 7TM REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="436"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="54139" checksum="9F307B1A3EFF9656" modified="2025-10-08" version="1" fragment="single">MKRTEPKVASNPNPRFPVEADTGAARRFLVEAAKQAARERDAALKAEQSENLHIGASNEKIASYRGIGLGIYFKLLEWLFWMMMWMNLCSVPYIITINTSVFTDYAHRYDHNYGVKVYDSFELASFTFAAIVDDKKDNGTLEYMNTEIGKSWKGPMRKGIFLTWIALVDMGSTVILSCLVFVLSVRIRRWWVRQKRETREVADYAVIVGGLPPYIIATEVAAYFERHFSRTAEKDKVMEVQLVLNYDKVFKACKVVHCNELYMNRLAELGDNTKGVALATPPQAIVSIQTAAAATAAATSAAAALTPSASVTLEMPPLKSAACPAVAEKSCSSSTGLKQPPGKAPEVIIREQEQFVRDLVQGNKLRCRAAIVTFNTEQMRAEVCKMMPSGWWASLFMRRDLRMSHGGRLWKVWVRRAAAPDDYRFENMATRIRTNILIQLFTGFIMFIFVVICAAFITWLSAASNKEARVIKWDMTELRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQS2</accession>
    <name>A0ABQ5RQS2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001734</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786:SF2">
        <property type="entry name" value="TRNA (CYTIDINE_URIDINE-2'-O-)-METHYLTRANSFERASE TRMJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="TRNA/RRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="6">
        <location>
            <begin position="358"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="269"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="329"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="525" mass="54921" checksum="8E18B1D47B80AA60" modified="2025-10-08" version="1">MFLLIAKDPMRFSPHNSRMSGMTALTLGTRAIRATPSTRHVSTIDWSYYSPFACKSDPRGRTKWYGLYTEEQYQAQRQSEERARWAASVRTLTNHQSQISWSDSPLDPPALLGGVTVVIISPRKPVSVGTVARACGSFECEDLRIVMPRQESYITRRHAKSASKGAQYILYRAGHYASIADATADCDIRIAFTRWTQASSPHVCKVDINGLTSHPAVQRVITQPLQLPGGDAAANMPRPPPSAAWTEAAAVAAEAVEARLMTTAVRSAAGMSGSEASLDEGYDFGPQWRPQGPEQEQQQQCCRPSGTVDGSGGYSNGNGSGQTLGPNCSTSDSTSSSSGSGSSSNSSSSGSSSGSSTDVYRPPRVALVFGREELGMSDEEVDSCEVCCSIPIGRLQESLSLSHAISIALCALYQARLQHLSSSTDVAASPLPAVGLTAAVAIAAEGDVTRCATHAQPDRPTSIGPPSPSVPVDTAAVAAVGASELGMSVGCSDGTYSSCKAVPVSYIVDVSTGAVKAAAATGTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRA9</accession>
    <name>A0ABQ5RRA9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Coatomer subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_002043</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COPG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1480">
        <property type="entry name" value="Coatomer, gamma subunit, appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032154">
        <property type="entry name" value="Coatomer_g_Cpla"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013040">
        <property type="entry name" value="Coatomer_gsu_app_Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037067">
        <property type="entry name" value="Coatomer_gsu_app_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF0">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16381">
        <property type="entry name" value="Coatomer_g_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08752">
        <property type="entry name" value="COP-gamma_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037093">
        <property type="entry name" value="Coatomer_gamma_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="13">
        <location>
            <begin position="38"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer gamma subunit appendage Ig-like subdomain" evidence="14">
        <location>
            <begin position="633"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer subunit gamma C-terminal" evidence="15">
        <location>
            <begin position="781"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI59984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="877" mass="96393" checksum="8429AC3AE7AE934D" modified="2025-10-08" version="1" fragment="single">MAATAQDSQAPLRGPEKKDEDREDETEFSPFYGIDKGAVLQEARVFNDPQIDPRRCQQVITKLLYLLNQGEVFTKKEAAEVFFGVTKLFQKQDINLRRMMYLCIKDICPGADEVIIVTSSLMKDMNSKTDLYRANSVRVLCKIIDGQLLAQIERYLKQAIVDKSAVVASAALVSALHLLGNNAEIVKRWVNEVQEAAQNKNNMVQFHAVALLHALRASDRLAISKLVTSLTKANVRSPLAQCLLVRYVAQVIQDSQPGANGEQRPFYDFLESCLRHKAEVVIFEAARAICNLRDVTARELTPAITVLQLFLSSSKPVLRFAAVRTLNKVAMTHPLAVTHCNIDMESLISDQNRSIATLAITTLLKTGNESSVEKLLKQIGSFMSDIADDFKIVVVEAIRSLCLKFPSKQRALMNFLSGVLREEGGFEYKKAIVQSILVLIQEIPDAKETGLGHLCEFIEDCEFTYLSTQVLHLLGQEGPKTKEPSKYIRYIYNRIILENATVRAAAVSALANFGVACESLRSRIIVLLRRALSDNDDEVRDRATLHLEQLEGKAGGPEAVQPRVDVNLGAMEKALADYLAGPTDKPFDISVVPKAPMSTRGVSALGLPSGAAAASGAPGLPATSAARKDPTAEYAELLRSIPQLAPLGEVFKSCEPVRLTEEDTEYAVFCVKHIYESHIVFQFNCTNTIAEQVLEDVSVAMDLAEAADFEEAFTVPLDTMPLNGVGQCFTVVSRQPGAIATGKVVNILKFKVKEIDPSTGEAEEEGYDDEYQLEDLEVTPADYVKPTLLGNFRKAWEELDPTTEKEDEYGLGTREGLQEAVEAVIGILGMQPCEGSEAVPPNARSHTVLLAGIFVGYQQVLVRMSFGIDAANNVAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRD1</accession>
    <name>A0ABQ5RRD1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002206</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF4">
        <property type="entry name" value="NUCLEOLAR MIF4G DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="5">
        <location>
            <begin position="909"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="282"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="113"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="360"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="374"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="398"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="437"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="500"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1136" mass="117843" checksum="A5E0C5A0DFAFB5BC" modified="2025-10-08" version="1">MYGTQGRGGGRGRRDRSFLGRGQGGGGGRRGPALPFSLLRELGDDTSGGAGGGSGRGFKFHGDGSGGGGGGNARFRGRGRGRGRGREGGRGDRGRGGMQFGAGGGDISGSYGADDRRKRTADRPADDASRGYKRQRQEKAAHSDQRPTKQRLMSTDNSGSGRSGDPGSSGLTRTLAAMKQQQAISGAGNHARPGPGSGFGSGSGSRGTSSKTKFHELLVDGWESRNGREAFLEELRLQAQLAKKLGKSKKDALDGMDKLMGFLSAGSGSLLPKYLDREARQEAEELLTKAGSGSGTGDQDPGLGSKVQSKGAKRPLSSAAAAAREVAEILDRESDEELELFGVDPDSNRDDDDDLEEEELLGRHGSESRSESGSEVEEGEEGIYDSEDGDDGGRELASESDGEEDGGMDDAGGEDDYDDDDDDDDEMEEGEEDDGYDMYGQLMASESADEEDVPASDDAAADEEEDEEDEAEEVEGGRHLTGGHRTNVKAASAAREETGSGNGARGGDGAEPAAAEPTAAVAAVAAQQRYVPPALRAQLAAAGGGGGGGGALDAARLQVERRVTGLINRLAEANLQSISREVAELYSTQGRRLVSEAVAMQILGAAESGPRASEQFAAVAAAFVTAVAAQAEAQDLAAGFLAELAQRMEAARSSGDSLAQANLVTVLAYCYSAGLVGPGLCYSLLGILKERFQELDVSAMVTLLNAVGLQLRNADPGLMKEFVLGLHERAAQLGRDGLLSRRAQLMLDLIIDIKNNKTSAAGGAAAAADVSLGGTATRKSKAAAAAAASGAGGGGRRGGALAVLQPSVAKWLQQLGVEEVSLRGLTWKKLLAPNKKGMWWMPVAGEVLDDPLLLAAAAGVGNVAGGSSAMAAAAAGAAAGEGSITAAAAAAASTAKLLQLAAAQQMNTDARRVVFVAIMGSEDCTEAHEKLLRLPLKGDQRREIVRVLVNCCLDEKAWNPYYAFLAIRLCGGDAGAGAGGGGSGSMGRAHRVTMQYCLWDKFKEVESMDVRQLTHLAKLAALLVARFTLSLSLLKVVDWSDLSAKQMLTWRIFLQHLVSSCKTAGDVKEIFARVAAQPKTLSGLISGLLLFLRTCFGPWVATKLPPGPESDELLKRTRVAEKELAAGAATAAAVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRH3</accession>
    <name>A0ABQ5RRH3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_001651</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18572">
        <property type="entry name" value="ABC_6TM_TAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF19">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="381"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="476"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="256"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="571"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="823"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="850"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="876"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="900"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI59706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="913" mass="97632" checksum="2B1C7ED6348F0248" modified="2025-10-08" version="1" fragment="single">MATVRVILICLVALLELGAVTWCSLWPLRDELAALARARGWWERVNFTLGNLDVMLLAFAKDLTLLVLVTSTPTHTTRRTGRGGPSPDAANHSGVRRAISGLCGLHAAFLLVKAAAVAVNAPAQLWPAAGGTAGSTSVGLVFLYVSIAVSLVASLASQGLGRGVVSEREDAWDEQQAAVEDEEARDPLRAPLLSGESTTTTNGLGTRAVAAADGKAGSTAGGAAKDAVSKAVAAKRKQRTLMELLRLSFVDWPLLVVAFTAGSLAALGSASLAYLTGQMVDFASIELDRSQFTHTTVLLLVVAGLTALFTGTRGGLFTIANTRLNVRLRKGLFHALLQMEIGFFDTNRTGEIVSRLAADTSTVADQVGLNMNVMLRSATQAAMVLFFMFAASWRLTVVTFILVPVVLTISQVYGKFYSKLSKRVQAELATANAVADEVLSTMTTVKAHAIQDSAEAAYAAKLKDFYKLQLLEAGAYSLYAILNTFLPNLVTAVVLFYGGNLVLAGVMSRGALVSFMLYQQSLTSAFQLMGDVFSALTAAVGAADKVVELMKRQPAISSGGQLVLPSLEGRVEAQEVVFHYPARPEVQVLNGLSLMAHPGEVVALVGPSGGGKSSVIKLLERFYLPTSGRILIDGHDLGQFHPKWLRRQVALVSQEPILYARSIRRNIILGLEPEDGCTATPSEEEVVEAAKQANADDFITAFPEGYETSCGEKGVALSGGQKQRIAIARALVRKPRVLLLDEATSALDADSEAVVQEALDRVMHGRTVIVIAHRLSTIQAADRIYVIGKGAVQEVGTHNDLLAAGGVYAQLVRRQLTRPMSQANCLGGSSADLRPSVSTTSLSSLMPEDNSNNSQPQQPEQTTRVTNSEEQHEQQHQPQPRRSPASQPSPQTQMEMQEEDQQHQHQPGARDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRS0</accession>
    <name>A0ABQ5RRS0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002379</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000007">
        <property type="protein sequence ID" value="GLI60285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="193" mass="21192" checksum="C9F6D90C9EE0A463" modified="2025-10-08" version="1" fragment="single">ENRFNEPKWKRRGVGILWKSQAVRDMSGREEGRKVSTKPASLSAGNVAQRLSEDLTRLFARLQLDDGDEWMGAEHLSSCQPVACASEFGPKWSDDWSDPYELSDRASALEHSLRRRSICSAQFSLDADESHSSCTATSSHVKLGSALRALDVSVSSEHTVEGPLGAPRACAGGVSRRLEQLPWGADFLPPYMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRT6</accession>
    <name>A0ABQ5RRT6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002224</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045277258" evidence="2">
        <location>
            <begin position="22"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="335"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="605" mass="60655" checksum="8D7611FDCA5F2E8F" modified="2025-10-08" version="1">MGPPRPKAWLAVLVLVQQCRCRVQRWHRRWEPRGSNSVWSPPGGLLGRRSDGDVRGVESGDEEVGQGVEEEARAGTPVGRLGVALGRAAAGTSAGGASRQGSGHRDEGPAGSGRQLRVVLFPAREEEDEAALMMEEDVRERENEGANVADGLHGGPSHAAVAAAATAAAAATSEVSFGHTESDKENNPVDLNEDAGKEDLPPRQGALGTPAHRQPLALQQQTQQYLSTPSGATGGITAAASAGRMQGPGGRASVAAGAASGASAAAAEEGSRGQQQEGVWGLDSGYGGGAVAEAMDVDGEGHNAVVRAEQGLAGGMDMNFGPADFAHGQIRSRGSQRTSAASAPSAQQAAEGSGAANAGDAAGAQGPAPQLATPLGSRRQVPGSAMRLRSQTRSQPSAQQGGHQHHAVGLVAGTPLGVGAGGAGNESMSHSEGRGLGSMLQRRQRPRFIDDDGVDSGGGEAGCGVSGGGYNGYSPGALSAGRSASQHGTGSKGRLCRSNLHSIQEEVQVGGTPVHLAAAGPGPGAAQPISDGRLTSIDPHTTGALRTALASGSGLMSDVDWSREQDESLATRTFQAFNIVARAAAVAAVAAAGIDGAAGYTAPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRY8</accession>
    <name>A0ABQ5RRY8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Iron hydrogenase small subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002484</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.950.10">
        <property type="entry name" value="Fe-only Hydrogenase (Larger Subunit), Chain L, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050340">
        <property type="entry name" value="Cytosolic_Fe-S_CAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003149">
        <property type="entry name" value="Fe_hydrogenase_ssu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11615">
        <property type="entry name" value="NITRATE, FORMATE, IRON DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02256">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00902">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="Fe-only hydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron hydrogenase small subunit" evidence="2">
        <location>
            <begin position="431"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="494" mass="52674" checksum="A83DE6D3F954BBD3" modified="2025-10-08" version="1">MQTALLSSRAGAYVPQPTVPSYGSKSVPVPCTPFRKCNSATPPHLKNKHYLNSGRAVLAAAAPAGTAAEPHWKQALVELDKPKDERKVMIAQVAPAVRVAISESFGLAPGATTPGQLAESLRRLGFDMVFDTLYGADLTIMEEGTELLSRLQAHLEAHPHSEEPLPMFTSCCPGWIALLEKSYPDLIPYVSSCKSPQMMLGAMVKTYLAEKRGISPNDICMVSVMPCVRKQGEADRDWYCSSGAGIRDVDHVVTTAELGNILKERNINLPELPDGGWDEPLGSGSGAGVVFGTTGGVMEAALRTAYELVTKQPLPRLNLSEVRGMDGIKETEVKMVPPAGSKFAELVAARAAAKAVDEAAASAGAIKWDGGAGFSNDEGANGLTLRVAVANGLGNAKKLLTKMQSGECKYDFVEIMACPAGCVSGGGQPRSTDKQVAAKRQQALYDLDERATIRRSHENPAVQTAYKEFLGEPNSHKAHDLLHTHFVPGGVDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSJ7</accession>
    <name>A0ABQ5RSJ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002745</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF517">
        <property type="entry name" value="RNA HELICASE NONSENSE MRNA REDUCING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="2">
        <location>
            <begin position="318"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="3">
        <location>
            <begin position="542"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="741"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="751"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="822" mass="88608" checksum="D34AB79CD3B851B7" modified="2025-10-08" version="1" fragment="single">MGPEQKRKGPVRRQRQRHAARLPIAVRRAVDAYVARYAPLVQMELEEERRQVEERLRLWPMGRLRSEGLVLTDLRAVRRGSYLGKAIVRLTAADGGELPFHRFTSGTLVTLCRVNPLSERTTEGLLLDRGRWEVRLVMDSLPPGLVEGLWRMDKGVNIVTYQRMAQAMQSLYKDPHAAGTAATRTDTGDYEGENDDDEDEAGDDENIAEYGGPSGQEQRYGHGRGGGNTGYGNRFGRGRKHRRNGKRRGQRRQQDEEDEDELPPGTHLGDVLLPPSPAATAAAAAQPAPLIPEVGLRWAAAHVAQPTDPRVADPETSLNVSQVRGVAMAMRHRVVLLQGPPGTGKTTTIVRFIKHIKHTMGYPHPILACAQSNVAVDNLLEGLVDLGMRAVRVGQPLKVRESLRDATLDARILEHPLLVDIDEQERQLRALRQRLPSLRGRDRGLGHRDASLLARKVREMRAQMMADILAGADVICSTCVGAGGDALQDLSFPLVVLDEGSQCCEPEALIPLVKSCRHAVLVGDHCQLPPTVQSQGAAAGGLSKSLFERLMEAGVPASMLQVQYRMHPALSAFPSARFYGGRLLDGVEPAMRLPPPLPLPAPDVAGPIMFVDVPGGREEVSQGGSKSNRAEALVVVGMVRHMLATGISSDDIGIVTPYLAQVQLLSNLLMPHVPGGAAPLEVSSVDGYQGREKEVMVFSTVRANPAATLGFLDDWRRLNVAITRPRRAMLLVGNADTLRGGGGGNATNRYTSATTTTTTTSTTTTTAAAEVEVTEATEAAVGSGSGFGSLAPSGGGCGVWRPALESGGHWAAYMSWLEGLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSZ3</accession>
    <name>A0ABQ5RSZ3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002820</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="665"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1092"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1305"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="526"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="923"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="923"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="940"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="981"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1468" mass="158787" checksum="49DF546586B376FB" modified="2025-10-08" version="1" fragment="single">QMFNTIVRREVTAPGSSWRVQVSPPSRLQPLQQLPGIASELLALTTALNSRPALLKVHLEVKENEDEGRERYSILVDLWPTPSVSGLLEVDSTGRIRTVLEEHTRPAGLLFGVPSTSLVGKQLAELLNLPADRVKPGDLLSLNNIKKSSLKSSSADANNVKVGPLHVLKGIRPDGIPINLGVQVVGKPGPGHVLHVILKVHQAPMLPAAATTLVNPSPAIPVAAERRLGASASLVKKSLTLATSAVTEIDPTGLFTELLAAGDLATPVITTAVPSSVRPSSGDRRPAISITAATTAATAGNNTKCNDEAATIAVVVPNEEGSLGSTFLAQQQGPPLLSPPILLGLPPVPAVIASPNRTQHLHLPVGVVRPVDGNDLSASATPAVGMSRRILSMTDDTMMKVVDLAASGMNKSLMITKEAISGDNIGDGGFFTRRNGPLAPPQGNNRRPPMRSGTPPPGQSAAEDNSPSCSGDQDGNHRSSSSKLDGDSASDVNTQEDGSGQPVRFTATPASERVLNWVASKGVFYQNSVPLGGNKSEDGFRPSDEGARSKDDDDYSPRQGGPIDIKSLLRKGMQDTDPPDDKSKPDAYGGVAYNYSPPPPGPRPNREMGGMTQDDDAASEGGQSAMSAQSDNRTDYKRGKRYRKLVKLMESGQTQQVQQRFRSHALLTVAILAVVHIFCFALTIYSILTKRKSMVELGRTGQAQRYMHSILTDVRSLDVIAKNKSLPTLYTRADIEPLITRISEGADEIQVRLREILNSHHSRHSPVHDLLFGTKRAAWNGNYRNGSNQYTELTIWDFSTRFIAMAKNVEQNLEAWMANNVSIADTIPGQFLIKSGPDLWSATRKVLDALMYLAVDDARWVDNLQLVFLSTEGVAITSIAACYLAYLLRALAAQRYKLYNTFMIIPAGLARTLALQNTSLELDDDDEEDEDEEDDKHSAVESTPSDAESSPGGNKQKKRHATVNLERNTESHIQRSGEYSMLSSAKRSENHQGPSSRRSKSKGLPPTEGTKDLVRQQHLRLSQQASLDLNGGCSAGGGKGYIRTLMQRMGGAFRRNISSAVTPLPQSEGGQPHLLAIGTSAKRKLKYDSHEAFMVVTPFVLWSLLVIVIYAVCVSRIMGIVEVVAVHSVSNFLAARTYRAVFFSQELVVNDDPALLASHRAALAGVHKVVRDAWYTLQLGQYAYIATGPGTEQFPYVKEGMAYASPELTDIFYESGRCHRVEGTCPTNHDYRFYQITRTSLDSMMQQYLMQLESLARDQNPYSPGISSPEFDFIYNVGTKDLIGGASEIEETHLNTIMHLFHGIFVLHIMLFILFWVIFGGFLVILLFPLLKRVSRERRRVAELLSQLPLELDVERLVARALGATLNVTSSGPAAGSIFQQVAAAAGALPAPPVGGQASTFGSFQPAAVESSGDREEPDTVNRWKAIIRSASQQAMAANRRSNNGEAAAAGISNGAMARTNFSASKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU15</accession>
    <name>A0ABQ5RU15_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein LTV1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007307">
        <property type="entry name" value="Ltv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531">
        <property type="entry name" value="LOW-TEMPERATURE VIABILITY PROTEIN LTV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531:SF0">
        <property type="entry name" value="PROTEIN LTV1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="408"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="491"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="525"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="58163" checksum="084BA4498F48FCD9" modified="2025-10-08" version="1" fragment="single">AHTAAAGAPAAMSTRARSVGSRRVARSVASTAQTHGSGLGPSVYVPAARLMPPKPDVKVVDARRLVVRPKGHDEAAAAATLAEVTALARHTELHGRRGRLYQDLDELEKSMVAMENGNDPNRAEQVEGDEWAVWLDEFVAGGGDGDDSGAVGIATAAINDDDDAGSGAAGMSEDPEEEDEWVLRHRHQQHLEEGKSGGHGSEGAATAAGGDGGGGGGGALSLASSYWRRPERRDRKEGFEVLDAQFERLAVEYDDADIGALEDGRYNDDDDDEEEEEEDHDHHVDGAVAVRKRSSIRAVPPAAAGTCKLSDFTDVLDEFLNEHRAAEALEAEGLRPIQASDDVALAASKPDPEALAATRARMKRQMEAQLAVGMGADDDEPVQTIVVQGVERWDCESILSLTSNLENHPGKIVEPQRRTGGKGGGLIRLSVKTGMPVVPGGMAGDSRGRPATAPAIQEDEEEGASGEGSGSGDEEEEGTGSVLDPGMLTRRKGETAEERKARKAAVKEARRNQRTAKKEMKTMFRQQATRAQKQTAGRSLAAGSTYVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUN0</accession>
    <name>A0ABQ5RUN0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="261" mass="27571" checksum="62AD24ED1C07AC13" modified="2025-10-08" version="1">MEPLLDNLTLAPGAQLFELDPPHYTSSYGRRTEAIFKHNVPPAPMLIAPDNGLVLYERVISTKAEHQMLDKLTKPKYYHPTPSIRKGGKGGPLGTAPSSGSGSSGTHPKNAARRPSSGSSAQHQSSGSRSAGPSSSTAEPDSRRTGGVSTTAPLGRETAQHRTRSFSAGRALPGATGHVKRKPLYPEDGPLKDYFSKERASVTATTVFTWGNRYKIGDCGTGRGVTGEGSTIGIAGRGSGDADRAAFTSYTPMQQKPPLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUN8</accession>
    <name>A0ABQ5RUN8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">U-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003677</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052085">
        <property type="entry name" value="WD-SAM-U-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46573">
        <property type="entry name" value="WD REPEAT, SAM AND U-BOX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46573:SF1">
        <property type="entry name" value="WD REPEAT, SAM AND U-BOX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U-box" evidence="1">
        <location>
            <begin position="330"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="428" mass="48154" checksum="FDC09FDADCA6AB7F" modified="2025-10-08" version="1" fragment="single">MARLRTARSNCYSQHCYGQRSSAGMTSICDICISINYLVKLLGEAAWELDNNCGLLEESRKLHHYLRRVEALISDIKPYLRGVRAWGSLRDVEGILSSLLQEILEAARKGPVVQYLQADRILKRFTTAAAHLDAALGHLDDVASGTSDGQNSGGWSGDDSGSRCEPTSLAVTRFSNEIQAELCNVRGLLKSAEFNLSAGHLANLQRFRKACDGLHEHFRNMMLSYSDMMEHTMQGQFLSKESFRTLINELRTEVEVVQQQMLQSVQLRCGKALCSGGCECGPGMWRGRAHPLHSSAQPEMERAQQGAADQHYLRMLMDVVRASHVNTDCEAPLDFLCPLTHQLMQDPVLLHETGHSYERKALEQWWASGHHFCPRTGLHLRRLNISPNHSLRSAIEQWRLCSDMHLSFKPVLNTLISHKQEAIPQEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVE9</accession>
    <name>A0ABQ5RVE9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_003193</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="193"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="524"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="459"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="462"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="750" mass="83932" checksum="681C392AC0C94649" modified="2025-10-08" version="1">MAVEEEKKKKKDKEEKRDKEEKKEKKEKGDKPKKEKSEKDKGDKKDKPEKDEGDKKEKPGKSEKKEKKEKDGPSSSNRPVSGRVPPPPPGRGPSARGAPAAKQPANDYLAGIDLPSSEEDDDYEERGRIDEEATRAPIMAGTSRDNKKLADKERKALEAAVRAKEDALREDENVFDVSYEGMGDEVSATATDVKVQNLTIRAKGKLLLENAAVTIAAGRRYGLVGPNGRGKSTLLRLMARRQIPVPLNIDVLLVEQEIVGDDRTALQAVVEADMELMGLREEEKQLMEQLQDEEKQPADFDHDTAQDRLNEVYERMAQIGAASAESRASKILHGLGFTEAMQKRTTQSFSGGWRMRISLARALYIQPTLLLLDEPTNHLDLRAVLWLEEYLMRWKKTLIVVSHDRDFLNSVTTDIIHLHDNKLHQYRGNFAQFEEMYEQKRREVNKAYEKYEKQLKAAKSGGGKDAKAKAEKVTTNATKSQVKRRGNPGDDGASSSAASAPQRWSDYTVEFHFPEPTELPPPLIQLIDVDFKYPGRDDFGLKSVNMGVDMGSRIAIVGPNGAGKTTLMNLLSGDISPVDGESRRSHKLRIGRYAQHFVDALSFDENPVEYLLNKYPAAGLKPEGMRAMLGRFGLSGQHHLTPICKLSGGQKARVVFTSIALMQPHILLLDEPTNHLDMQSIDALCDALSEFEGGVIVISHDSQLLSRLCEDEERSQVLVVEDGKIRHYPGDFEDYRGELIREITAELDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVV0</accession>
    <name>A0ABQ5RVV0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003857</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43574">
        <property type="entry name" value="EPIMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="33"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="384" mass="43279" checksum="0B576A0236071C66" modified="2025-10-08" version="1">MAADTVHEQYASVSKLAKYPFEPYWLEKKKLKICVTGAGGFIASHLAKRLKSEGHYIVACDWKRNEHFEEEEFCHEFHLVDLRLFDNCKKVAEGCEHIFNLAADMGGMGFIQSNHSVILYNNTMVSFNMMEAARVCGATRFFYASSACIYPEFKQLDTQVEGGGLKESDAWPAQPQDAYGLEKLVTEELCKHYGKDFGIDVRIARFHNIYGPHGTWKGGREKAPAAFCRKVLTSTTEIEMWGDGKQTRSFTYIDDCVEGILRITKSDFTEPLNLGSTEMVSMNEMMEIAMSFEDKKLPIKHIPGPEGVRGRNSENKLILEKLGWEPTVSLRDGLKLTYFWIKSQLEKETNSGVDASKYSHSTIVQTGAPVELGSLRKADGQEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW14</accession>
    <name>A0ABQ5RW14_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PspA/Vipp/IM30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007157">
        <property type="entry name" value="PspA_VIPP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31088">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROTEIN VIPP1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31088:SF6">
        <property type="entry name" value="PHAGE SHOCK PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04012">
        <property type="entry name" value="PspA_IM30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="123"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="287" mass="31627" checksum="5BCB1147484C4AD2" modified="2025-10-08" version="1">MQLTRVRLGPAGSQRSAFRPAVVGVRRQGRKAVVVQANLFARVSRIVNSWANNVVSNAEDPEKLLDQVVEEMQSDLIKMRQAAATILAQQKQIEAKYKQSQTTADDWLRRAELAVQKGEDDLAKEALKRRKSYQEQADTLKAQVDQLTVASNDVLNNTRALEAKMSEARSKKETLKARAASAKTSQQIQEMMAGLNTSNAVVAFEKMEQKVLAMEAQAESTKMLVGSDTIDNRFKALESGTVDDELAALKRGMLSSSSTPAALPEGRPIMDALDLELEALRRKARAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWD4</accession>
    <name>A0ABQ5RWD4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004439</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14951">
        <property type="entry name" value="NHL-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045302">
        <property type="entry name" value="NHL2_NHL_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46388">
        <property type="entry name" value="NHL REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46388:SF2">
        <property type="entry name" value="NHL REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13905">
        <property type="entry name" value="Thioredoxin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="253"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="2">
        <location>
            <begin position="728"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="138"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="946" mass="100188" checksum="769C5731A9B6D074" modified="2025-10-08" version="1" fragment="single">INNIICFLWARNICSFNFTPWWLIKYCVAVDAASKKPLTTRTDSVFIDRIHTYHLYEFYSYLHLAKIMQHNMLAPGQPTACRAAPSLPFIYKVNRSNLPFQKCRSAPEERPGGAPPGPTAIASTTSSIAGSPHDSPEPASTSPSTQTEKPVSAPTLSSITGSSDNRIEQGWLNQPFLLPAGYRTTRRDLLKLTSLAGAAGCLYVAASRAKAMSFASPQALINALVPPPAPKPLEEGTDRVSAFKRYIADLERRGGGREVPEFPTGSDWLNSAPLRLSDPSSTRGSLAGRVVVLDFWTYCCINCIHVLPDLAQLENQFAGAPVTVVGVHSAKFDNEKDSSAIRAAILRYDISHPVVNDRSMSVWAALGVSSWPTLAVVSPRGRLIAMLSGEGHRQDLEDLITAALQYYGEKGQLDGSTPLPLALERNKMQPGAAASPLRYPGKIASDVAGGRLFISDSNNHRILVTDLNGRFIEQIGGNGPALRDGSFDSTALNRPQGLTFSPRRNQLYVADTENHAVRCCDLVAKTVVTLAGNGNKGRDYRGGRGGSAQPLNSPWDVELDASEDYLYIALAGQHQIWDLELSSGTAALFSGSGAERNQNGPTPFTTSWAQPSGLSLGGDGSGLMYVADSESSTVRVVDLGSGGSSLKVGGDPLFSDNLFRFGDKDGFGPEALLQHPLAVLSSQNGSVVYVADSYNHRIKALNPSTNEIVTLAGSGTAGFRDGVGMEAQFSEPAGLCLGPDGTVFVADTNNSAVRILDPVTGRVSTLALTGVPEPRVDPLAAIASGAAAPLAVPAGFQLVRAQYPLAVGTSGNSLNITVRLPVGYHLTAGAGSSYYCQVLAAVPAADATAVIVRPSSGQLPDTMEPSVTLAVSLSSSSGLGGGGRGDDGRLLLRVLAKVYYCQQNDVCLFEQICFEVPLDLGATRGASTVALRYDVVPAVQPAFVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWY2</accession>
    <name>A0ABQ5RWY2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Copper transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004627</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045474027" description="Copper transporter" evidence="8">
        <location>
            <begin position="23"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="65771" checksum="EBC45BEA12317655" modified="2025-10-08" version="1">MAPSHPVSLFLILSSYITFGTAQSICNTHTWDSRVLHEYCTVAGGAAYGSPACSIYRLCDKSSAASPFLGPGLCSLDRLFVTLCADRPELPECTTSLHSTSASGQARCVRATALKLWSTEAVRGALVALCSRDSGSGVTAVCKVCNGDKCDTPDLLLAYTALCTENPTATDCEPWAAFCAKETPAMAAALCLSSDATVLAGTGRAHITLRSTTAVQVSPPPPAEPCIQDATQASCKAYTYPKSSALADIDRLCGSMPDMPGCAIARACESGQHHVADKYCLPFVLLATLCHDMPGMRGCENYKALCKPGSVVQQCIVEGPVPHAPTWSQARKAVFAACGDHPMGGCANCTSNNCPDPLASLADICHEMPEMAACNGFWGFCEAAGQQDVAEWCGEDDSKYLPSMLMYFHQRTQELLLWKDWRPRTQGQYLASVLIIVLMGVVATGLKTAKGALVLQWNHQRALKGEELLVVSVWMPRNDQITEIMAKSAITGVSLTLDYFNMLIAMTFNVGFFCAVIAGYIAGTLLFGHVLENYAAILHQQRRDVAAHLKRRRLREAGVVGGGSGSAVSLLANGNGVNGGAAALGVHMMEEEEEEVEKEELLMVVEGEGDCNCVHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX29</accession>
    <name>A0ABQ5RX29_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PI3K/PI4K catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004542</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031559">
        <property type="entry name" value="SMG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF124">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15785">
        <property type="entry name" value="SMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="50"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="5">
        <location>
            <begin position="2654"/>
            <end position="3016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="820"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="921"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1381"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1575"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2050"/>
            <end position="2131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="3486"/>
            <end position="3515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="3896"/>
            <end position="3947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="3960"/>
            <end position="3985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="4015"/>
            <end position="4067"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="2991"/>
            <end position="3060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="820"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1408"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1458"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2103"/>
            <end position="2114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="3896"/>
            <end position="3905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="4028"/>
            <end position="4042"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="4542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="4542" mass="471000" checksum="B523EE1D4FEC7B18" modified="2025-10-08" version="1" fragment="single">[encoded data, 4542 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXQ9</accession>
    <name>A0ABQ5RXQ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_004582</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17328">
        <property type="entry name" value="MFS_spinster_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF79">
        <property type="entry name" value="PROTEIN SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="456"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="43"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="485"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="496"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="513"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="547"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="571" mass="60019" checksum="7E5BAC5509BCE928" modified="2025-10-08" version="1" fragment="single">VTCRVPYLGKSRALCTGYECIMVRDRIDKMTATQPRWFTPLRLLGIFCLTNLVVYLDRDTNAGLIASNGVNGSARTAENPGGSGIQGDFNLSYFADGLLPALFMVGLLLSSPIFAEAVKHCSAFRMLGWGMAVWVLAVVACGAAPNFSFLLVARAFVGVGEASFVSLAAPFIDDFAPAAQKARWFAAFYLCIPVGFAAGYIFGGAITTVASWRVAFVAEGLAMTPFVAFALAAQPLQLRGSKPADGTHYPPSHNQPYGHRRWRAIVGEFLTDVATVFRQRVWVSVCAAYTAYVAVLGVYAYWGPKAGRALFFGADDRGGSADLVFGGVTVLTGVLGSVAGGLALDRLGSTLRNATLLCAASNFMGFVFLLLAFITSRSFGAFMGIFAVGQLVIFLLQAPVAATGMWCVPAELRPLGASLMTVSIHLLGDVPSPPLVGLLQTRLAAGKDTQQAAEQWRISLSIFSLLLLVSGVFFLIAAWQSGPGSDYRKQPVGAGDAEDNNGDDGEGNEGREGAGGGVGREARRGGLGIVGDVDVDEVPDARPLLMGGESTGSFSSSSSNGGRGKIPDAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXV3</accession>
    <name>A0ABQ5RXV3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Potassium channel tetramerisation-type BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004392</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18316">
        <property type="entry name" value="BTB_POZ_KCTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF136">
        <property type="entry name" value="GH08630P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="2">
        <location>
            <begin position="103"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="570"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="715" mass="74966" checksum="FA2E6E77C46764F1" modified="2025-10-08" version="1">MRRVSQRSTWYGGTYVAGSPSILTTPCARVLARPRVHSGAVLLRVRAVLEEPHGAAGAPSSGRGGGGSGGSRPAKQSSIRWARRQRMLVSEPRLGSRDDDLLELNVGGKLMQTTRSTLQVISGSRLASMFRKGGESALRYDSRGRIWLDLDPPLFELVLRYLRETKMNGGNRRGCLPAVQSADVVPFRRLLLHLGLLPYAGATIPIAVGREPTGGEVETPLPPTIHPHATTSAAMSAAPSEQPGRGTTPEGHFPKHHHRHQKQQSQQQQHPTDAQIENSADFINDTATCNVLSSVPTTRGNVKGSSGATGSGCGSGSGVGSRSEESHHPGDQSGSQAEPQGDGTIGCQRENGVPSATFRTSASTSGSVCGSVSPSPSPSPSPPPSPSPSPSPSSPAEGAASGGRNLAAGRSGGSNAGGFRLLPPRRGGDSGGGADRAIMKLSPPPIRTVGGVSQLFYISTNYNEARGLRVSVHTEDDVRAAAAAAAAPPVAATQMPPPAEPFDIFIDTTYGEGQISLTFVSRSELLPGGVFLGLTSRAQLDRKGAQIPCYGWKSNGCTYNDHTGGGRLGGSSSSRSSSMGTESILHDPAAGTTGSKGPRAKLGPTGAAPESGGGASLRMPQPFGSSEDAPQGPEEQTPLWRKGDSVELIMRQGQQHNQLLLKVNGQAVDVINRLPSFRHWSWYVGLWAGGDISCVVFQRDQYAVYWGDYAVKRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYE0</accession>
    <name>A0ABQ5RYE0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01299">
        <property type="entry name" value="Met_dep_hydrolase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051781">
        <property type="entry name" value="Metallo-dep_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057744">
        <property type="entry name" value="OTAase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135:SF3">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="2">
        <location>
            <begin position="207"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="614" mass="63908" checksum="67769EBE5E3BCB78" modified="2025-10-08" version="1">MLKLRLLTDGLLAMRLPTSRGGDRPDYYSSNSSDNLMPEGLCINSFSRACAMRKALQQFRATTTTTTTKSPPASSFSALATGAGLYEAPWKKPCLVQTSNVPTEPEEQGAGTAPAMLPSEPPAAAAAASPASTSQPRRPFLELPLLLTNCRVLDVMEGGYMEGLQEVLIQGRLIKEVRPMGQLAAEAAGAEGERVAPAVEVDCGGAVLMPGLCDAHVHCTAVTADLAGLMSLPESYVAMKSAHILAGMLARGFTTVRDAGGADFGLAQAVEEGLVLGPRILFTGHALSQTGGHGDFRGRGEEVCACGAALRGIGRVCDGDAEVRRAARDELRRGAHCIKVMASGGVASPTDRLTNTQFSEAEMTAIVEEATAAGTYVCAHAYKPSAIQRAVRCGVKSIEHGNYLDETTADCMSQRSVVLVPTLVTYHEIARRGVASGMPEELVAKVGDALEAGLRSLSIASKSGLSVCFGSDLLGDMHSAQALEFELRSRVLSSPAILRSATVTCAWLFGMQNSLGQVRPGYIADLLLLRPGLDPLADVAVLATPGGAAVAAVFKEGLLAKAPLLARAPAGAGCTAAANLDVKRVSSEAVDGDGCSSSVMMDGWADLNQRLLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYH9</accession>
    <name>A0ABQ5RYH9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47852">
        <property type="entry name" value="OS06G0298400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47852:SF2">
        <property type="entry name" value="WW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="319"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="635"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="862"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1027"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1229"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1333"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1432"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1539"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="262"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="635"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="887"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1033"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1076"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1451"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1470"/>
            <end position="1479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1569" mass="156867" checksum="09045A6CDA8B61F4" modified="2025-10-08" version="1">MKRGGHPAKKARLELDGEEEELDPIAAAMEAKRLRLLEASKGTDAPTSDAITARPLRVDSAPDHAGGSGGAAPLFPLVTTTITRIQRQRPTMARTTAAASAANAGLAITVVEKPAESGDGGGDGGGALAALLGYKDAEISTDDEIEDLAVEERTGSGGMVIGSGAAPGPHTHASADAVAASASLLGPEEARTGTSAVAHGREGGGGGGGGGGTRRAQEKKAAGATAAAGTGAGVQDSMDLELASFMHELEASGLLGEGDIAGGDEAAAGPTGTVTATAGGGGAAATPAEPETVAAASPDSKSEDAAAEQKVLGYLIHAPDWSKCLDTGSDKVYYWNLSTGEVAWKPPHGCDPDLLLPPEDAKAGDSGGGDDGGSAVVVDAPIVEAVELKGSLQPTGQRVGAGGVSEGDEAGAHAEAASVPGPSLVEAEDGVAAVEVVLAGSLHGGVEEASKPQHDEGPADMGVEQSIAEDTRAGDSASGADGKGAVSQCSFGAQLPRLAATTVSLRPSGDTVITAGDVHMEAMDEGGGGVGKVMASSSPASSITTSATLHQGPSAGGVECRADQEVEVGQGIHEEEEASGAPAAADRSVDADAIRPSTKAGPAATAACRKPRTQAGDASRGPLGARGVAVGAASASAGRGNDTAGVGASAGTGAGTGAEAEAVDRSAVGTEADTELLQAVTDLLLRPPQPHIHQVMAALEAEAAAAAKCFLQDLPPLVRLAVEAQVRAADWRELSAQQAAAAVTGQPADAVCWPTLERHIVSRVKELLEALPAANAETQRLRLSTYSVTAPAGTIDRLGTDAATTTVAAAVAVPSEGDLESEPASRQVGEWAPCLAAAAMAEDVLLPSRGAGLAGEISRSVAETGASGSRIAGSGDASREGGGPGQADEEDMEEGELPETATRRSAGCTQGVMAGGNEADLPPQGLTTESGAAALAVATTSATGTPTAELLVYGGSVSTAFNMQGYYNWPAHLYRYDMSYAMTTHPYQLGSFQQDDVATAPTVAQALAESVEAAGVEPPLPALLKDGADVAVPPLPSSPAPLPAEPPPPLPPLPPGHDEGFPPLPLDVPPLVESASTDDGDDMIIEDGGGTTAPGVSPPPDTVPPLPPGFESAAAYIDMYGQNAYDAYMSQMYGYAYGMAPPPATTPAGHEALQYVNAYLGYGGAPVVDHAAPAIAPPVPTGVEVATAVPGLLAATAVGKPPLPQLKRKEYRAEPVRNPVAVGVEDAEDVGDTDHGRTRISASPAPPPIDLLNMAGVPSGLGGGGGGGAGSGGGVGTSATSAVAAAAGGVVKRKVAAVGGGGGAGTGAAGGTKRSKGLGGRMGALVQKWSAAQKQLEAEEEESARKAAEAEDPGAQARKRLAELERWKWEQEVTGASRDNANFAPLGDWRSRFPKVVQQQQPDGDADAEGAAARVQPVAAVAPLLPADDATAAEAAKKMRKAAKEEGRREKKEKKGKKEKKEKKEREKAAATAAPGAAASASLPSVSTIAGAQHQQATVLNFKTKPDLDALSAGLPPGWRAMWDKATGDVYYGNLVTRVGTGMGAPRTRTGVASRCGPRAGRVHRPGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ14</accession>
    <name>A0ABQ5RZ14_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046653141" description="Sulfotransferase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="33908" checksum="6FF35D530EC62A6E" modified="2025-10-08" version="1">MYLFVVLFLGVTTLVRPIQPFDLYNAENVRYADFPRRRAGPRLATEECVFVLATPHSGSTALMDALNQIPNYLIRGENWAAHYNLFNAYQRMSSIPQSSGQSYIHWREHRQASFHTVKSLYEAQAYHRKLPFFHEFQIDRVLIATRAYYKVLFGHFGNGVVAGFKETRYVCGTAFGSNQCSEQFSAYLHFLRRTCIRTKVFLLSRTDYSVHANPDIFESLTGGQKLLAERDLERTHQLYDDYVKNYPEHAFRVHMEGMFDHKRNATLARNILTFLQEDPSLPIQFTRMPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZI4</accession>
    <name>A0ABQ5RZI4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="300" mass="33117" checksum="6A82E59DD83EBB37" modified="2025-10-08" version="1" fragment="single">MVPWSISAVKNGDVQEPSRVPFKPREAEQVLSEAWMRHADSKSGINSKPSTYRPQIGLSRYQFGDGEYQASVAFFQQHHERQAEHEPLPAMLELVQTTRTLARTMRNRLTTGCGQVGSGSGSGGGNGGGCRSGPMSLASLRRRHSAGTHNNIAAQMFNSSSGGSADGGADECGRGRGCNPERDPDDSEGVQEFNDSDSDSEDDEDDEGDDDDKEPDGGGMAEVSVGSSNVNSHAFGNPRRRRRGRRRWRHRCYFLSATPRCPSSSDPLRDAAIRSFRWCRQRRRRWSVLTATKRDATRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZT3</accession>
    <name>A0ABQ5RZT3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein tyrosine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PKY1</name>
        <name evidence="10" type="ORF">VaNZ11_005772</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="2120"/>
            <end position="2424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="877"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1037"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1472"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1538"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1579"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1708"/>
            <end position="1744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1760"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1837"/>
            <end position="1856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1889"/>
            <end position="1922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2457"/>
            <end position="2514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2570"/>
            <end position="2625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="880"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1049"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1085"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1472"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1541"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1590"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1722"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1771"/>
            <end position="1784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1837"/>
            <end position="1847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1889"/>
            <end position="1903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1904"/>
            <end position="1921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="2484"/>
            <end position="2508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="2594"/>
            <end position="2606"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="2147"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2625" mass="268317" checksum="346932E92C3062A4" modified="2025-10-08" version="1">MELSLAAVSLAVVICSLTGEFAWTRVLLSACALACIFGAFLVELMLSKATVLANSWLVGPEQVLSFGAMFLAALASRAGASEHISHLALTTLFWHVSLPKHDRKGMLVLVAVLQFAQTALVCGAHLAFRPLGAGKYIFIVSAGVVGILLPVNWLLPRLLSSAHTPCDPNRKSTAGDSGPASSAAGERFGNTSAACACARGISGFDRCHTTDAISPVPGRASEAAASKAAQLSRLIALDSGVRLRPLGTSSDGYNGRARSLGFRDSAASTQVSSWEFRGREGPQGRNGVPAPPYCQPETLSFPAASTATTACQTDDELLLWLFTGASTVPSAADEVRDEPRASYSGIMSPINLGFQFAELWSSTAKDGANVGGIRVGGGSDGLRSQDGGGGNSGGGDGGRSGGVGKYDSFTSVWSRCTDGVHDMVLSARASSGEGEGPDNLATASARHLEEWRYSNGSQQGAKPGGQQQPASAASSSSLVAGGWSQMPWHGYAYPACAKQSSLGVSGAGAPVALTQMHPRNTGVRGGGGAAADGSGIPGDGISTGFLSTAHLTQHHSQQLAMHSQQLVQPHRVFLRTQSSGSGRTSGIFGNVALALGSSAAAAAAAAATAFSGTAFRSVGLPADQRGMTAAATVLGERAAMGAGGESVSGPGSGFGSVSGSAAGAASLACRALVRQQRLQRSRTFNSLSPERSRALIGLARTDAGAPSQLPPPDLDEGQLGSVAVSVPSPYSEESDEEAVQRLRTLRALRRYQPELATSLESFAALLRRRELDGGGVVNRASRSFGLEETAMPMAGAAGNVTAARRSGFPTVAAAAATGGFPGDLVAMGMGEEMALCGSGLRRHVRRTQSSSGLLCGLAAAGGAEVPHREPSLRRGGFMHHQQEQHPHQQRDHRHQSQQQQQEHQHCGRQGTRHHASTTTNIPRRSVQLNGDLEAEFLAGVVAAGPLPQLIDASELTLLEGLPAIQAASAASQAMPPPRTTSSDGCRSVVMDAHSELSFEQHLLVPAAAASGAAAPYSHSATSSRRWSCASSSISSATVGSRGERMSMSAAESVTHGLSTGDGQKQGATGSGGMADYVRRAHQVQRSRSAGETGFRSSDANRRGRQRHWSQLPHQEHRRASLQQAWQTMIAEGSLRRATAAATSAPLPVTARMSDSDGVLPYGADPHGPPAVDGACGSGASAASVSPTGRSPTSPFVISAGVTSTIVSGRHSSGDGSGPVTSLFARTAGGLDSAVVDDRSVRLCKSLSERSLQQRLTTSHHYRRVGRTKSRFALLQDPTDGSAGGAGSLTGSSMQRISSGGSMAVRQLENVLEEQEHEQEEEQEDGPLAAAAAVASLRGSVAAGSRSTNDAGERGPSATVVATAATSLIKSERLKSCSGRNEDDGCGDPAVKPDDGCGAVDAGFETAFAKAAAAFGSTTGDDVVEGAVLAASGAAGAADASGTRSPRRTAWRSASELSFASVVMRRSRPSVGFSVTSMDRSTSGGLTPQRDGRDRRSLDSFGESAQAVETWLQEGDDSIPSRIETSVLSIDGATSLSPSLLGSGGSHGGSGGVAGSSRPSTSGMMLTDGIMRQRESIRLSMERKSSVARSRGGGSSISGRLSGGSVSGDGSADSDEELLDVLADEAVLERTRKVSRAVLRRLQEQNHVSVPCKTASFRFAAPIIPSLSSSAPLPTTSTLSQGSGGVPAPLVPLPTAAAAMEVTSPFTVTHGTQDSVARPPPGGAAATPVAAHPKNPVPDDNIAEGILLPPLAGPVLPELERERTRERPVGHSSTALGGDAASASGSPPPPTASGHQSPEMPAVVSAHPAPLSAQNLQHHTRHWEATSPAACDPAALALQLPSPSPSQSGPMDPLSDLDASPSAALIAAFNTAAETAVTAPAAAAASAVGSGSSAAPHQSGVESGRSGGGASGSGAGGGGGSGPVPRSVIAARLSMPLLPFNNTDATSALVVAAGRNSTTSALSRQTSNNTIGGLSACESTGSLADAAAALLMQSGDDIASASISATAAAASRQQLTVEIQHNSALSRSPSVLISGSNAGRSIVVRLNSGGGGGGGPGPLSGGSVAMFGPRQTFAPSTVRQPSKLSGGAALSGGDTVELEVEEAVLANRRMQDDRGVLLEEIAIDKVLGFGSMGMVYHGRLYDTKVVVKLIEHGTGVLGKEKERGRLARVEACVSRMLLHPNIVLTYDACTGSLQPGRLAAKLGRGGGGVGARGGRQRFMTVMVQEFCELGTLKDAVSKRKHGLAAWSGASPAPGSLPLLYQVALDIANGMKHLAALRIIHADLKAENVLLQKMETSNDRPHGFCAKVADFGLAMVLAPGEDEIRQGIHGTVSHMPPEAMKDTLFSLATDVFSFGVVLWELYTTESPYRGMAPHEVVEAVCVRGERPGWPQHSPPDLVELAEDCWHMDPHQRPSFADIVERLRQLLAPTRGPPERCGSLTSGITEESDELEMALRQQRRGAPVTSVRGGGGGGAVSDTGSDFVGLPGTGGGGGGIPTQGSSRGAGAGAGAAEGSLGVPRAAPRDPLGLLLWALCQFPAEPPLRVAGPSADSEQWVLQVEDMVLKSRQEHEDLPAAGSGTGMGSGFTPAAGSVPKLESVSEVSPDSSNSGEAAGAVASTTPVVKPQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S014</accession>
    <name>A0ABQ5S014_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="55317" checksum="C36C47DFF1556142" modified="2025-10-08" version="1" fragment="single">MEPLNISQPRRRTMDNSMLGFAGLFGAVNPVQAGELNFPLFVPNYQVHRGQNAALELLNLPHDYLEMLVLHHVPQSSLGALRTSCRQLKVIADRRLTQVTVPYDNILRHSFWRTSALPTVTTVVLEMPARVAAVGAGTSITASTAAGFSGVSAVHDTGMLGPAATAAPPHPQTLLPPLKPLLQPQGLVWQHFQPVPLLHTTPVVAPLPPPQAVAAALAAVAAAQPLSSLLPPVTFSGLRRHLPGLRHLKLRNVYFPSRDAVAGGSSVASATAAAMTSTAAAAAAAAAAAATGTADYPLDFSDILSLSVEGLGMRSGAMWRMQPANPFPSGLEPSLARAASSLTSLSLSHVTFEGHSGDVLGCLTALVDLSIRSCNNFTSNGLTVLGQLTRLTSLAYVDVFYKTGIPNEMVVLSALTRLRSANFSRCDLYTDEVAAWATWGPTLTRLEMSSKWQMTLSAVPVLLPYLTSLEDLDLADHCLDRRMWRTVSSRMPRLMTARFSWVNLPDRDDTDDEGDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0H3</accession>
    <name>A0ABQ5S0H3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CobW/HypB/UreG nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF46">
        <property type="entry name" value="ZINC CHAPERONE YEIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="2">
        <location>
            <begin position="6"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="573" mass="59609" checksum="F167E73EE6D05536" modified="2025-10-08" version="1">MKLPIPVNIITGALGVGKTTAVTHLLGHKPSGEKWALLVNEFGALVIDAGLVEAGIGLDNGEEARRDAAGGSRNLSSTPAPGIVVRELAGGCLCCTLSGPLGVAIAQLVRQAKPDRLIIEPSGLGHPAGLLDTLYGEHLRSALDVKAVICLVDVRAAAAAEGELVGPAGDSGGGGEGAPSSSGSSGGGWALGTGLPAAASLETIQDQINVADVVVGHKADLASPQQLDAFGCWAEQLYPPKAQVVLASHGRIDPALLDLPRAPVFRPMFNPQTHRHRRRAAQRAVKAHTPCPPHSEVPPGATAAAAATPAAPASATDEPSAESLASGLATIKLTADPNPDPDLGMPDDLVGTGGQAEALPQLQLKPGQPLRFPSAQPHKSYPQSRLPLPQQGEHRHHHYNHHQQHPDVGSGKVEGVDVAGGEEPQVVSCGWLFSSEEIFDRGRLGAVVSELWRGAARVKGIFRVGKSSYVVPYFRPDGNVELRPVAYRRESRLEVIVLIPASRRDVIPQDMRGASSPLADAEATVAMAGCPALETSSVATEPERGPVRLEAAVAAADWDAVERALLGALQRRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0I9</accession>
    <name>A0ABQ5S0I9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046299364" evidence="2">
        <location>
            <begin position="25"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="143" mass="15436" checksum="8CB6D19689710DE6" modified="2025-10-08" version="1" fragment="single">MSLLPVTSEMRLLLLLGVLAPTFALPEVRELQFAVNEHGFQNEGALHQQQQSGSLRTQQRQATEVTSTSLDNIDDDKSSSESTDIGLRVHPDGISHYAVSSGLTRQLLQGKQVLPSPPPPSPRPPPPSPPPRPPPPSPPPRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0L9</accession>
    <name>A0ABQ5S0L9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="663"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="876" mass="93977" checksum="F956824F193655C7" modified="2025-10-08" version="1">MDAAALARENTLVQEASKLYPVLELECEGKRAICLGGHENIIDSVNVLVEGECILISEWVLEHCLGLRNGTTHRPQEDWMLTYTKPYVTAYDNQPVPKMPLLDWCRHMCEKNGIENLRSGTRLLVFWWDMEHKSVHKLESRGKTWWPATYSPKYDKHGIWYSARVLKYDRATASLHIVYDYNSPADDLEVGVVTLPLNYVHFGPAPPARGGQAQVFPLPWKPRTTYSQPNPAHQRAPPAKEDDSMWQGTGASVLGQAQGRQIKQARRQPKEQDECRIPLGPAEKEQRISTGKDDTGSRQQQPVDCLILSPTVPSQQHEPAQKPCHDTLVQPQERVELREQQPQMQAPASTSGRGILRARHELDNEQKDFTERSSKKAKVASKLSCPGGTSAGKNEDVQGQKQPASGVKSALNNSRNYGSHAAVAGATKIDGSGLADIQAQLEGQKAITPAATKHLLPPELKLPKADRTSGASTATDTSALKVSSSLKKGAKPGCNTGVRGECFTSVANDGKIVEASKVSPGNGQQQQQGHWKSEQSNHQKLAPDTQQGPSSAVAGVLSANARLSNSQMRGAPGTPVDDTIMCPAGGSSRHGPAATGAAGAKQKTVNTLIPPRLHLGPTSRVYWPRSYPGISICRTADMLPQPAAVRLPHPVCEVLLRRVHKTSRRAEAASKAGAKLQQPQSRPPLGGGRGSHDEHLKQQGQTILSDMDIDDDVAAVNDHAAAVVDAVGRAGRASTAMDGDKPAADLACDDMAHHSGAPAIPAPAAPAPAQNISMAAASTGGVKACAGGSSSTEGGDYGKNGNVLDDDGHKIFQVRPITQHPELMASPEHYGSDGSDAAYAYIDMMEYAVKMPSEERLDAYETLAREAMASSLAASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1A2</accession>
    <name>A0ABQ5S1A2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006338</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF54">
        <property type="entry name" value="SPHINGOSINE KINASE RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAGKc" evidence="2">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="310" mass="32203" checksum="580932628522CB40" modified="2025-10-08" version="1" fragment="single">IVAAGGDGTLNEVVAALIKVNAPRNIAVGLMPMGTSNDFAAVTGIPQDLESALRLCCQYDNLKQLDVGLLNGEVFMNTATLGASSELGSETSHEAKKLLGPAAYLVKGMARLTGYAAVPVNMRFPAPLESQQPHHKEMVKEVVVGAASEGPAAAAASEAAPAAGGGPLESQQEAQPAGKDEPHVVRSSSASAGRGKGQVKGWFRSWKKTIEVREEVLNLTAGNSRQVASMLQACPDALLDDGLLDVTYITGGAVESATALVGEILTRGIDGAQPGNIHILRVPWIEVTLEAQDPHLTWPGNRDGEPVPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1L3</accession>
    <name>A0ABQ5S1L3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)</fullName>
            <ecNumber evidence="3">4.3.3.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006487</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by PDX2. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively. Also plays an indirect role in resistance to singlet oxygen-generating photosensitizers.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00343">
        <property type="entry name" value="pyridoxal 5'-phosphate synthase lyase subunit PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="PdxS/SNZ N-terminal" evidence="10">
        <location>
            <begin position="8"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="294" mass="31445" checksum="1892D9199D9B737E" modified="2025-10-08" version="1">MASDKGTFAVKVGLAQMLKGGVIMDVTTPEEARIAEEAGAVAVMALERVPADIRRDGGVARMSDPAMIKAIRAAVTIPVMAKARIGHFVEAQILEALGVDYIDESEVLTPADETHHINKHNFKVPFVCGCRDLGEALRRIAEGAAMIRTKGEAGTGNVVEAVRHCRAVMGAIRQLQTMDDDELYVYAKEIRAPVELVRQTKHLGRLPVVNFAAGGVATPADAALMMQLGMDGVFVGSGIFKSGDPASRARAIVQAVTHYNNPIVLAEVSAKLGEPMVGIDIREKGFNSYAQRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1P5</accession>
    <name>A0ABQ5S1P5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006522</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="7">
        <location>
            <begin position="131"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="436"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="655"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="664"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="779" mass="81541" checksum="90AAD61586054729" modified="2025-10-08" version="1">MSADQEQPQAKRVRLSDATSSQQHISAPPQGAAPFGAQGPGAGAGASSSSTNGPSSTTGATVALPPTLASAPYQHHNQDEAKPSVSVALRPLTAPVSITAATAAARAAGSGSGSTAAPGAVAGSMKPTSFIADARETVFFRAVSGATADAITAEIAAGGKAVHVDFLHQHFGDSEQIRGYRGLKITIWIHVRTYHTWVDIQFAGKRPGADKLGPIFDGAFPSGYCRSQDQFVATAAAAAAAMPDLLTLGDCVGTLPLPPPTYGIKKAEAADSSGDLSEVSVRRFQVATAPPEVKALHARLEPLLLFTIDGAQFIDADDPQWELLLPVARAEDGGCLVLGLTTLFNFFAYPTSCRLRVSQVLVLSPWQGLGLGKALLKLSYDLAKSRRCADLTVEDPTPNLQRVREKLEVEMMRGLPWVVRQARKCLDAVARNETTWPGWEEAGSRQSSSTTGAPQPQPPAPKQEPKAAAAAAAEVADEISEGGEGGESRADTSVHPLLADHAFFAALGAAPLPNMSLPAAVHAEAIAAAVARWQQRAAAAEVVTADAVAVKAAGESGGGGVGEGEDGQAAATTAATTAAAALTPTPSFLNAICAELKMHRGQIRIVWEALLWCEADAVNRPGVRAAVEQLIVQRLESQHFCCMGPAAATKRLVEIPATDTRRRRTEEDKEHEAGGTGSSGADDAQENDLGTSFFMYRPVGQVAADNGSGSADTAVVATGRLNLTHVTVEDKARRMEEILQERRLQLESLAAVLSASKINNNNNNSAKMKKLQLLMQKRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2T6</accession>
    <name>A0ABQ5S2T6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007405</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="29"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="342"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="450"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="468"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="483"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1291" mass="137918" checksum="A63278A8AA3ACD4D" modified="2025-10-08" version="1">MSSIGLALLLANPQEDYTFLLRQVPTPSLPEEVLVVVMLHLDLHDVLACRLVNKSWSRAATSGVRHLDVPCKALAATVGADRVERADAMLRRMQQVWSATEAVTLSGLAAGWALKEELSLQLLMNHLSCWRNLREVHLRGMPVFPVATLPLPRRLAPPHRLGRPAPPVYVHSGRADGAEAAVRSGVATVTATTSSDPSGVARSQNTSRDMRVRRADAGTSVCGDGGYNGSSTPGPWRLECLSCCLGLTRLEVELVPVKQQLSPTSPAQFHGQHSLGCCQAAARACGLADSQSQQQQTIRQADVAQLVAGQAALLEGLTRLTRLTHLSIRLLDLPAQSIAGRGAVAAEDSESEQESEIADLDEGGDEDEDNSASEYVHDIDDAGIDYASDLEMDLELGQDLVLNSDREDGNAPAVDGVKSYRFAGNIAMGSTSGGFTSNTLIPPQDGMNGCMVAADADSGVGQHEASRRNPQLHDTQTLAQPTSQSSDGSDSQGYGSSEDADGYKRSSDGDDVLGNGGSWDAAAGLAAARPASRRRRLGPTVMESPMGNWIPHPAVFVRMFSRLRSLRLTGCNLCGGGALASFANTLTCLTSLSLQGRLGVSHKDLAALSGLKDLHDLTLSKVDLLYDPRDPDDCPVALMAELYCALQQLRNFQSLSFDVDPGGSSSGIHSAYLLPLYDSPIPRLRSLSLSMILDSDYSFHMLAELTSLTALNLSYVTWPLALQSDDMTLLAPLTGLRRFSMQADPSERHHLISLSGRVVYELAKAWTDLSQLAFRGQIRADIPGGPSLEFLASWTSLRDLSLSAVLDEDDTDCRLVYTNNNNNGNRGHGDRFTRDPTCASGGLVGAGAAAEILSFFYNDDDDNSKAAQDAGALDLGVGLARCLPPGLTRLALSGLRLGGTELLQLLSGSLKGLTSLQVLDCGLTPAHFMVGDCAASQLQVLVLNDSHPWRACSSRGHEPKDWEGPRGYTATAVAVTRLPHQYPGSTSRCDHSTGPSCLTCCPPGAQPLFLNRTSRVYSCGSCCCCCLPNIWTSWNCFGGPQTQIAYAPHPLNTPPSGANSGKMAGVAASVQCKPSGRPTGGQIVQQSQWKAQERLRVRQVLPQCQQRHLAYCSCMGVSCSAEGVANGDLKKSDNDSLGRETADRRNEGKPVRFTCLTAYDDWSYADGGSVAELEHEGGCGGLGERTCSCWRQCLIRHGLRSLGALRKLEFLLLGFPVDELAAMDVLRPLAVLTGLRCLTVHACGLQATEAREVSEQLGRLAQVRSLHLAGPRADSAAAQGVIDAARRLPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3J5</accession>
    <name>A0ABQ5S3J5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007163</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family. ADAT3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF156">
        <property type="entry name" value="INACTIVE TRNA-SPECIFIC ADENOSINE DEAMINASE-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="178"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="230"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="370" mass="40009" checksum="A80C18B3CC5E72FF" modified="2025-10-08" version="1">MAAEDIAFEMAVLIPSKTELPLRTVLLAVAAFPNKVGNLLMKALGAAAPLAELKHLKRVRKAPDDGALLEAILCALDDGEVLYTQHGGQRLRLMHGAALAPQTRQQWETWTKLWPITWRIPDNGTPVLVETPVEEHTQQYFERHMRTALQLASVSCVDNAALIVDPDSGAPVAEAADRSAVHPLQHAVMAAVQAASERDLALWPPVLASVDPYSGSGVNAMDVIPDANGSSEEAVTLRQSYGESNLAPDELRRELNGGTSGKDAGPGSTRDGDEAVEGLACKRQRLDTGPQGPQASARSRPYMCTGYDCFVVREPCIMCAMALVHSRVQRVIYCQPDATFGALGGCRRLHACKSLNHSYEVYRLGLKREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3R1</accession>
    <name>A0ABQ5S3R1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dynein axonemal intermediate chain 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">WD repeat-containing protein 78</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_007767</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Dynein axonemal particle</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF12">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="840"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="133"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="304"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="458"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="764"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="29"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI64501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="992" mass="106217" checksum="AB0311D1FD84DDC6" modified="2025-10-08" version="1">MSDQKTPTKTGLPPRPTVPGQQGAGYRPRQSSQWSPGAPRASTTGQPRLSMSGATRGSFRKSTRQSNRSQGGAETERLQVVIDGVDRTPKPLLSLNPTVLKGVQNDTTTPNSESSEFFMDRMSTAAFTKAGWYSNADSGSHTPKSETDYDYETEKKDRERKAAEDAAMAASYGDEGGGNESARGPVREDEQPPAPVLLSERDLERSVYLTLHETETIFIWQAAGVTVAMDTEEAKAVEEANRRYANLLHTKQATDKYVDAEAQTLVALTKSRDVQSAVLVTRSAAVQATAWDISDTFRALDEAAADAEDDPRGGTVSSSLPPISQMAGGVAKYTGGPKQSGGDASARGTTAGGLGRTSMFTGGTSFMSGPGNQASGHMSGLSSNLPNGVPVEQTAPAPEPADPLHVLAGLPEALMLMDNAINQNNYLQQLLLYRDIQPLPGIRSSVANAMRGAVSGGGGGAGLGDDADSDVLSHTGGAPGRDTRPLSAAPSDGGRSHHSESAATRSGPHIQPRLSNPPGQLSRQPSERSIPYSRGMSLRDGHGGGDARPARHSHGGGGGGYAASVAESNWPEELLQELADLGDDAPRMEHLWDWVCPMTAGKNVACMGWNKINPDLLAVGYGSYSFPSGNAQTGADGAASSEPKGLVAFWSLKNLQHPLWSFEVKAAVTALDFSTYSPNLLAIGLYDGTVAIYDIKSRQGTPSMESDVHSGKHSDPVWKVKWLDHGPERDEPLVSISTDGRVTQWSIAKGLEFSDLMKLKRMARRGAGGPPSTSAATAKENGAKAAAAVPEQQDAFISRLTSGMAFDFSSRDERIYVAATEDGWLHKCSTSYSEQYLESYRGHMGPVYQVQFSPFKRDMFISASGDWTIRMWQEGRDMPLLTFQASTTEVNDVQWCPTNSTVFGSVTANGRLEIWDFSLSTVKPVMHQKTTMKLSCLLFAPSYPVVVCGGEDGAVKAYRIFNVSHEYDTLQEQLERLDECIRANVMKSQAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4V4</accession>
    <name>A0ABQ5S4V4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007598</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 4 ARMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="303"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="561" mass="57916" checksum="B9A82EF38A12A146" modified="2025-10-08" version="1">MMRTRKRPSKADAAADFAESDTQEKNSIAINCKTSRSTSQCLSSVSSSTASSDAEAGPCPPGRGAKPGSPHARHAVVIDHQGAAGKVVHKPDGTVIWEPAPECDDVGGRRNKEWLDAAMLGVGTMLLYMGYLSGGLGVIQGIEAVEVATRAARPNHLTTRVVTEIMYRNVAGCFAGVITGLVMGPVLAARSVAGALARPALVGFLLHQGVTRITRWRSGVSGLLRKLQSRDSSKVLEALRAIIAALRRNPRFAREFDKLKGVEVLLQRLAAMMPDCPILHLIAQALAELCKDQECRASLVAAGGVPRLVALLQNPNPVVSGFGLSALARLADNPLAVEAIRDSGGLNRLVELTAAAVPAMSSSAIVVTNPMVMGKDQSGAVTAVQQTSSAAVLTAQPSVIPVPVLPTAGGAYDRRAVLLPAVKLLQALSLDPASKAAIGVAGGIPALLDVVARSEPRSETQSEAIAALHSSLRGCPDNQRTLAALPQASEVLRAALAGYGPCWHVAKGDLHAMLNVLARLQGVQEAGGFVVVQSQVAAVAVRGAAAGPASGAVIAATVGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4Z7</accession>
    <name>A0ABQ5S4Z7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007781</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="34488" checksum="2557A60C5A550282" modified="2025-10-08" version="1" fragment="single">MSEQLAIYRRCRRQYPGTAACVLLPAFCRRKAEALLRGMRHVHRFEPGTVLFNGVDREGTLAPWPHLSYSVDVWYEAPDGGAVGYGPALSALTLSAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVAEVNALGLSPESSDCPQVTLADGGKHPIVGRITLSLSLGPLRVVTRPYVLQGLAETAPYIMGSSTLRQYRARIDMETATLHMCKGTLSIKVPFLSFEHVTPKEGETEAVPMVNFAVTAMAARIEPELVGRKEAVRLLRKGAHGLLLRPRVSCCVTTAQISADPEVEALLSQYEDVFKDIPGLPPMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S527</accession>
    <name>A0ABQ5S527_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008424</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21218">
        <property type="entry name" value="CH_PLS_FIM_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056343">
        <property type="entry name" value="CFAP47_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053879">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058952">
        <property type="entry name" value="Ig_CFAP47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45912">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45912:SF3">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24529">
        <property type="entry name" value="CFAP47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22544">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26579">
        <property type="entry name" value="Ig_CFAP47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14874">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="1573"/>
            <end position="1684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1039"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1831"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2056"/>
            <end position="2082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2574"/>
            <end position="2593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2919"/>
            <end position="2943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1039"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1840"/>
            <end position="1852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2062"/>
            <end position="2073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2932"/>
            <end position="2943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2943" mass="322642" checksum="1026006B6E71502B" modified="2025-10-08" version="1">MSQLQVSPPALDFPNVEPGQVLSTWVTLKNLDARTRTVKVRPPKSKYFALREFFKPTCLAGGLETRFEVFFDGDKFEADRQSVQDKIVIQTETGSYELQLKATAPKAEVRILGDLIMGLLPSDSKASKCIQLVNDGAAQAPFRVEWDRNLPITVEPSIGVVEPGDVPVDLVLTVTVGEPGSLASEISVIMGSITKKIMLTATIIKQSFDLVDSSGALLSELDFGSLYYGQTSEREVTLFNNGPVEARYIISYGTLAEMRSKVDDEAAANADPNDPFADVIMAARQRQKARNTGDNPFHITPLSGVIAPYSKASVSVRYTPTAPQASKGFNCLVPSSDQALRTFEYFAVVELLGLPTKMKLPIRARGMPGGLRLSSQSLTFGDVDTHSWADQLLGVTNTNSQMGARYQMIKSTPYFSVDPSSGSILPGATIQVVVRYHPKAMGKHKGKILIKVLSEQGETIQELNLELSGSSLRMGTKGPLVGGTDKLPQDFERSHNFVDEEQAMLSTLEARKKSTRLLTKPWDKPELGDAYREISNKETMSRAEAQAAAAHKEKYTAVMRAQRTAREAQRKNGRVAENDVNLGMDTRSGLMAPEPKLNRTIDPLWRVDSTKEAAPRMLRQLGQGDLEGAHQWKARPVSEQERIDCSRELSKDELARLVVGPRMVDFGKVSMGSTNTRHFVLHNTLDSFINVVLDIKHVEMLRDTPCISQVIPPGAKADFQLVLQATEIRSVNEKIEYVVNGNHVLSFPLSADIVPVNLDFSAEDLNFQFSLDNWDEAVEKILILDNPHKFPVSFTVESTHPSFLVSAGGGGVIRSQSSVEVVIRWSPDLMGPPKQEGFLIIKMVGGEAPKRIYMTGELPEGSVRPKDKELFVSVVPVGIQQCAVLQIKNHGSRDTAFRFQPNDQLLAKPNGGRIAADETLDIEVFFTATEPGVMSNALLLEMRGGNIVKIPFRAEAVVPNVEIEQDEFSFGSVFVGSSSRLPLTLHNTSQVAATLMCDLVAHPDLQLLCPKDAWSTADYEACPLEAIGATGGQVLGSRFGSARGSRNGSRRQSSSLGFRSMSHDGFKYRITINPGKELPLLLDFRPMEQRERDVELAFNLVTAGGNAVPPVRKVVTGRGMNPRIILSKTLVDFANKVVIRSNQMKAPYVIDLYVTNQEEKPVVVSFGEPFPAPHSAGLPVKGIYTMDLTPMQPLEVGAQEMVGVQLRFCPRDSRAYEALIPVYLDDTTDEPYLHIEVQGVGQYPRLSFDMRECLLPPVPLGLRSTATFYVINNGYDNLELKYKLPADEGHLPMEVEFPEGTIIGLAKDRLPVVVSFMSPKPMSFTANIDFMDEDGKRFSMSVTGTTDNSLLTHGAFMGVNKSQLVFSHEPGMPVFLEEAAYVLPPPHMCLGPETSCRGVSRYLNATTTRGPFDNLGHQMMVSRGKLLLEIVEMLSGKTVPGKIFKLSNNRKEAVRQLLQQYDAVQVFLKSHGCLLNAVKPEMLLEYEDFCRVMNERSTAAPDNSDELEALDMWSEVEANFQVVSAQAWNSMVLQIFKVFVLGRVTLKSLRAMPGSENLELPPDSSIIGSNFYSVAESALLAWMSAHCTREFGPKFQRITNFDSDLSDASVLFALLSGYWPGLEKKKKVLRLDCRDEQDKRENAEMVLKIMTDLGLPYEITVDEIVNPNPHDMMVFVLYLHHTLPQFVPRSCIDFACKLGETQCKELELTNPSKKAISYSARLEGHRDFSIESSSLRIEPKISAKLVIRCTPTNTLSQTSRLVLMSKREGGVHAATLVFQLACKVNTRAPLKRVRTEGPLYELQMFEFTVTNPFPADGDFTIQVLHEPAEPLPKKEEDPAAAKISPAAAARSRAVGKRSGRKAQQFETPTAEEIAAKWGRLWPDAFGLDRNRLRLKQNASERIKGFFLPFSMTTAYCTVLFRDKDQGEFCYELVGEPGLPSPIMEVKGNVPLESPQQVYDLLLPFQNSALEAAKRAFQEKHPLAKDKEQVALLKQDPFKGNEITYVVTQTNSLMNAPSHISLSARSLLIYATEPQLPASPPSGGALQSTASGATGIVGGASGAISNRSGGQRNPNVLPPPEPNTLRMHLSPIGTGIYPTRIMLTSPLDVRVIDLELTAQTMTQNFVLEFNTSARQPITQDIPLVNTGDTNMTVSATINGNTWSGPREMIVPAGGTVMYPLQFRPLLPGDVKGSLELAINATGERNTYTLLGRAAEPLAEGQIVIECQARKPATKQFNVPNIMGSSTEYKVMCDLDFMVGVPQVKVPPGLASQYKITACPQRSGTYNGIITFTTADGQYCWFTLEVRASEPPEVGTIDVRAAVRTAVVVGVTMANPLRKDMELAVRYSDVALCGPSVVSLPGTQEPVNFEFFYAPLLPGTSSGSVILVNDDVGEFRYLVNMTADPAPPDQLPDMTAELGRSSRSPLVIENPLDVAVLLSATSSNPRNFAVMPSMVALPAYGSAELMIEYTPSTLDHEEEATITVDGDVVGTWEYNIRGHGELPTVMEATTMSTMLGGQGQTIITWRNPFPEAASVSVQLRTDEPPGVFELLRLGPYNGSSSSKALTGINSSSANSIHGGVPGAGPGNGAPNLSGVISLGQRSSTLTVQESTVQPSGTLNVPLRYTPAALKTCSCEVLVAVEGSSYSLNTLVWRFPVRALTEASLNGVVFRYKCKARSRIEETMEVVLTGLQKVGPEDEFTHEMVLPTEHRAFLEQSLMVEPVGPSPVRLSRPDVPLRYKVQFAPQRMLPPTSLELVINKSTGGRWRFEMQLQATEPDLDGTLTIEAAVGATSYVPLRLYSSSQEPQPFTANFPSDTPLVFNVVPSKGLLPPMPRPGEPEPEPPLQVSYNCREFGKVVRGRLHVQTSDMHYSYELRGRMPTYVPPSPSHFTPSVDNRLDPSVETRLSLGRPTGKNYVASNMRATKGTHDGGGQTGPNNATTAAKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5X0</accession>
    <name>A0ABQ5S5X0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF54">
        <property type="entry name" value="NUCLEAR TRANSPORT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="2">
        <location>
            <begin position="24"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="794"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="801"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1224" mass="123943" checksum="41A75D373BDC2EDB" modified="2025-10-08" version="1">MNEQFRAAVAAVYGHDPARRQAANIWLDAFSRTPEAWGCALSLLEHTSNASVEQRFFAANLLASKTRSDWAGLDPQQRSQLAEAFGTTLRNMLLPPGALSPSAAVSLEPVLLERVARLAAAAAALGGLATAERHLTLAQELATAAQPMLGSAAAPEANRSAGEALLRCALLVLQQLAEEAEALDSVRRKALLAGPDGLGGAGGLAAQRPGVVALTRDVVASAAGWGTSVGSPGVSFPLLLSAVRCLSAWVWLDESGYADGGLSSGALQAMSPGLLPSALSLLALPPSGPPTPSPLATSQAALREQQHATSLYAAVSELAAELLGSGSRTAAAAAGGEAAEVAAAAAAVDVLISIGRPLATAAAAERAAEAAAAGGGSGNGGEVGAALAALLAWSRVAVAVAERNAGGLTSGPGERAVQLADCVVGAWSACPDCREVAEVCADYFLAVNTVPTAERNPALTAPLYSALLPHVLRAVSYPAGFQDWETEVEEDEEAFHWFREQQAADLLETIWGQCRTAMVSQLVAEATSTGPGGAAAAATTAHGGGGGARGPHVSDWRRVEAALFCLRAVNVPVKAAAMGGRAGALDNAQAAATSAELQALLGRLFGDICSPSGRVAGLLVVPWVCLAVSRLIGEYAAWLGKTQDAPLQAAMGLLIHALSVPTAAPAAAQAFRNCCVRGADKLTSDLRVLTALATAAMAVVAPPPPPGGGAGAAVLLGLEERSAVAEGLSRLASGLPAEAAVDAACGLIAPCVSRAQSVAAAAAAAGGPLSTATLAALAAELGLMTAVVRFLEPPPGPHRIPPTSSSSSPSLRPGAAAAAEDHPALRALQVAWPVLSAVAGEPQCQTDPGVVEALAELYKCSLMSTKLAGRPLLPPLIGAMLGVLRVRPHAAVLECLAAVVELFGEVAHSGETRNAQIAALDGCIQMMGALMANLSAQQQASSGAPAATATSPFPSDCSASELAAAFFSLTDRYLVFARDLLMTGQGAAALPALLEWVCDVIVSMREREPVAAALSFLSHLLSAAARLAAEETSGGVGGSGGDGATAAETRARLDALFGRCGPRLVHSLLVCGTDTCPPQLMRPLAGCLMGLITLADAGTVAAPPPGVVSEPPPISPGDSELRRGLLGWLLGSWQLPPLAGLIQRGWLGTGGEHALLFTSLMLRGHYLQADIARAFATAPGAVAPTIAAANMRQLPRGRLDALVTDFFRLTRGEADADVLLAYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6R3</accession>
    <name>A0ABQ5S6R3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045080142" evidence="1">
        <location>
            <begin position="20"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="450" mass="46462" checksum="1FE7A0FFE74D7D87" modified="2025-10-08" version="1">MSSLVSSLLLFLLLRRGSCFLEMEAIENGNKLPKDPLSGLGRHALNQVLQSRSVRELLSTTSQQTCIWQPPKRSIASNGICGVNEYFILTMRGAPPSTSARILAYTFYKLHVCSSYSSEPDCVANSSHACTWDGGAYAGGAGDSGHCHLSMSAVFDPEVLRGRLYCEGSLLDAAARCALAAPGPTSCASASASLAASGAAKGGVSGGCIFANASQLANLTNVARRYLRLASLVSYTSPDLEGPAGVGDGLCVAGWLWQQSTLASLQSTLANDPAYGLLQRDLRGHCDGTDWLAKALAGATAICTAAGSSASVADSQRDSCLAIQGCSWDDGSSTCEAGYEMLVDALYDMNDAWIRALSNMLNQCNAASSSFACSAAATAMDVDPTLMPQLANVLTDQSWVMPGSRKGQGSSAPRAGGAVTSWLLLLVSTLTAVLLGRGGGGLHGGGVDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S701</accession>
    <name>A0ABQ5S701_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_008427</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI65001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039999">
        <property type="entry name" value="LYAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014898">
        <property type="entry name" value="Znf_C2H2_LYAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13100:SF10">
        <property type="entry name" value="CELL GROWTH-REGULATING NUCLEOLAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13100">
        <property type="entry name" value="CELL GROWTH-REGULATING NUCLEOLAR PROTEIN LYAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08790">
        <property type="entry name" value="zf-LYAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51804">
        <property type="entry name" value="ZF_C2HC_LYAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="119"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="325"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="491"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="176"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="326"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="442"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="509"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI65001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="626" mass="64178" checksum="CF9D414D3975E32D" modified="2025-10-08" version="1">MVWFHCEDCGDTIKKPKLVNHYRSCSASRFSCVDCLQTFTKATVNGHTTCVTEHEKYALAATKPGGFAAGGLQTNREGGPAAGAPSDPTGLEFLSTRPPWKCSVCNVNCTSRETLVNHAAGAKHKRRSRAALAAANGGNKAGGNGSAANGGGASSRAGDGGAPAAIGTRAGKRQKSSSSSSSSSDNTSSSDDEKAENHGTLAKRQASGARVTVKGNGVLAKRNSSNSRSDSESIDDSSSESDSDSDAPAAKKQKVEAAAKRPGRPPASAPSDSSSSSSSDDDSDGDSEDSPTKRAAGQKAGCTAGSACKSVQAKVGSNVIATLPPAAAQKKQVSAKAACDSASEESSSSDDSGSAGQTGQLQRNVIGSEKGTGGGIKLPRLPSNSSDSEESSDSDSKGQPQDLEKRVSIRAAAPESAPAQKVNAPSYKNPSTRRRNSSSSGDDSDSDSSSDDDKDDSNRGRSATGKGQGTGKGVQAKVVAAAPVLAAAVNAAKAKKHTAPKPGSSSTSDSDDSSSDDSSDSDAVKVRSPNPKTDILHLSLQNVKKFARLTAALKEALERKAEVGRGVLLRLVRKHLLAESELEVTDVELRKVRQQLVAKGVCELVNQTRLTKPKVVAAKKSRKSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S839</accession>
    <name>A0ABQ5S839_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zinc/iron permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009635</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045891">
        <property type="entry name" value="ZIP9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16133">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39 ZINC TRANSPORTER , MEMBER 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16133:SF0">
        <property type="entry name" value="ZINC_IRON REGULATED TRANSPORTER-RELATED PROTEIN 102B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="487"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="608"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="240"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="315"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="366"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="370"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="629" mass="61442" checksum="14A0395AF56BBD25" modified="2025-10-08" version="1">MPGPGRRILCVRCNTLPPFSAHHFVRKKAVNLLPSARSVGIHNRRLPSPSHFASPIRTRYSSGTQSYFRRRVFVSTVALPVATTASSTPTVIIASAPTAVHAMASSVSEGAGALWATCLVLFAGAMLTGLPPVCWWVPSPAGKAAPATRIAALLQGPSLRMLSAGLMLGSGLGVVVPEGFITFVEGEPGGGSGLPEWSAGAGLLAGFVCMLALQLWLQGDGHTHAPAATAIYVATAAPTAAGGSEGVGTGDRDRGPEGSGYGRSSAGWRRRWDGGYSALPSAAAADAEMSTVRFPSGPSVAATAVAVSSGVTGSMELTRRHGGTLSAAGGGGGGDGDGAAAAAAPSAHVSFSCALEGVVPASALPKPGCAASPPSSSHAASGVGDGDGSGNGNGSGGAAIAASSSLATPGSVTSGGGVSSAKLALGGLLIHSAADGLAIGAASLGGGGGGGPSALSLSVAAAIMMHKLPVTLGLTTYLREAGWRRGAVLQALLGFSASAPLAALAAYYFLGMLLQGTGRGTADDPSASGNGVSGQVVALAVLFSGGTFLAAATQHILPAALAAAAPTVAATDMGLDKHLTNVEGAVLTEEETFHVGGVGRGCGTSLPLLWLAVGSLLPLLIAALLPEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8G6</accession>
    <name>A0ABQ5S8G6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14494">
        <property type="entry name" value="PTP_DSP_cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057023">
        <property type="entry name" value="PTP-SAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22784">
        <property type="entry name" value="PTP-SAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="2">
        <location>
            <begin position="188"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="317" mass="34125" checksum="8718D0295FDB9664" modified="2025-10-08" version="1" fragment="single">SSKHLKEHRACSANCSQTSCTNRVLNARHTGCDVNLDDLLPPIRRGASSKNVAYKSGGMLRSDAYPQLPPLPKLPAKEMAGPTPWSNWAIPGRVIAGAYPASLDDAETEKILTTMLELGINTFVCLQAEVNINTPEHAWRAGHGLRPYIKDAQKILSKAHEQGNSKITQQKIDFLHLPIIDGNVTTDSAMNRLAEDCMERILRGERLYIHCWGGHGRTGTLVAILLGRLYGLPYTNALRYTQAFHDSRVYPQGVRSPQTPVQRAQVRRLLASQSAGSISSPGSLNPMSAARGIAPVRAPVSSSIGVGGKPIGAGAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9N1</accession>
    <name>A0ABQ5S9N1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="291" mass="30657" checksum="0ADD46A2BAD6AE7E" modified="2025-10-08" version="1">MPKVANHNDAAKTGKTSSGFLDLDLDDVIEIVINSDDCHDDDFMEPKIGIFNSGHEVFVVCGLSKAVPGFRPAVTCNGFLASLSPTSSGYTIVPRGADQQLSFKYEPPSPKFSCRQPSSISYRLQLLHGSQCLYSLRGLSKVLRCQRSAHALEWGPVHLVFAAAADLDTFLATLKYVDVEEVAPVMEMQQLTVAPTAATAGRSTSVCGCSPSRQFSHAMQRKEGHRGHGSNHAHGGGSCGCPSHSCAPFFAATAGAAVLAAVAAACLLTQRGTGPPSGRKRVAECPASVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9V0</accession>
    <name>A0ABQ5S9V0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sfi1 spindle body domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052270">
        <property type="entry name" value="CACF_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22028:SF9">
        <property type="entry name" value="SFI1 SPINDLE BODY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22028">
        <property type="entry name" value="SFI1 SPINDLE BODY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2201"/>
            <end position="2241"/>
        </location>
    </feature>
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            <begin position="2457"/>
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            <begin position="2201"/>
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            <begin position="2222"/>
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            <begin position="2272"/>
            <end position="2286"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66401.1"/>
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            <dbReference type="Proteomes" id="UP001165090"/>
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    <evidence type="ECO:0008006" key="4">
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            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="2590" mass="296352" checksum="2E7968DAF6F3717A" modified="2025-10-08" version="1">MARAFQTWRQNTSTRRISRSERDQALHNWAVATSFWAVHLLRRCFNKWRTLAPRKALMAMSIWGDNSRAGCFRRWLVFTRFLRSKGAQGVRLWRGRLIQRCWRRWRVFTWARQRKARAIKRARKLWVERTLRSCWLQWQDFTSDRQRRVLASEKAIAFWSDRRLQSCWLEWRSFTTTRRRLTLAMRRALAFWSDRQLLSSLLEWRQFTLDRRRKTLHMSRAIKFWTGSALQACWRQWRGFAAASRCKALATQRALGFWSARVRLSCVRSWRRYTIDRLRKAEATDKALRLWARSSLAACWSTWLSFTAKQQHKAVGLAKAVGFYRDRTFRNCLFEWSLQVESARQNMHLAETMRVRRLLHKWQLVASSLAKLLTAHDTIQQADRRRLLLASMQTLADAYAARDEASKRLRLALVRLQTRRLRGAFNKLRAVSIRGQRKRERSDMALEWWSSRCLARAWTAWRKAQESSIYATEAARDHALKILRPLLRNAFYGWLGWHEGKMMRRSREVDACRTLTSRKQHLVLGAWRGSVLDNIQLIAACQSIMRNEVHRLMVAAVRQLRREHDHARRAAEGRRVVQGGAARRCLRHWKALGQARQAQDELRIQRLRVAAAQRIRRILMAWQLVACLHAEHERLVHVCIIGMNRRWAAAIIRAWRGTVLDSVTMRMKMLAALLAWERGLKVRAWRAWNALLAASRASACRFAMAARHYRTRRLGLCFGALSTAWRAYRERLVRALRFRTLHTRGLVAEVLAAWKELVEVLKWKNMRTRRAVTHARHRLLAGSMYGWYYITRHRVESKHTVAVAVLHWVRRRRAATLSWWRAWATTHRALRLRGEGYWMVRAGRIKAGVFLRLRANVVRNLRIAAAVTHRNRYMARLALSGWLLRTLLAAEWVSRLQSVGTQLFWKVLEDSLATWQAYVVHRRRIHVMMRTAMHYWRLGRQRSALDSLRWHATRRQIMRAAVVRGCQRVASRVLRAWCTEAHYRRGLRDRFALLQFHTQRGTLKRSLDRWRIFLVVLAFRRDQNIVAVQHYLRVMMWTAFRRWYLYHMHLRHATERAQAILRRWRRRDAAVVLAAFAENVVFRRQMRDAESVFRITCSRRVFAAWQEFLGMKRATAFWRHKRLTGIVAAWRQYVRWRRGLRAIGAHVALRWRSLTAALVLATWRDLTRERQRLMELGFILATQTTRSALSALLLAWSEYATRSTWLKCIAAAIAARLRAKTLSTILTTWHVTAVRRAGLKRIAAAVTARSRHAALVIAFGFWYWHALNASRLRQISEQLSARTQRGLMATAMVAWCSHVGRAVRLRASHEVIATRVRLGALRATFFTWRGRTAYKVHLKSIEHQIIAKRCAYTSARVIGLWHSYASYKAHLRVAERKISRGLYVREAGIVLGEWRARAAYKARLHRAEKSVTLRIRMSALEDLFSSWRNWTQYKARLATTGAQVAWRQRRSWVIEVYSAWRSYAAYRAKLRGALEKVRTRRHRRMLAQACTTWRLYMLRMSALRGLLERVLMRATGLAFYGWREMVADAKRWRAIQEATRARLEQKPALADTACYAAARLRLWPLSLAFYTWYDNSKECRYLRGKASDAIFTYAGTLLRKALAMWLAYALRRRHMRQKLNRFRAVAKTKAQKATFRRWQSLAQYHRTLRLASRVLKEKVDGQTLRSAMQLWRATAARWGGLKGILARSMARLLSMAWDGWIEETIAGRERAQAVQVIGTRWAHRILADYLAAWRRLVSAVKAVRRLLARMQRRRKLWSWAAWQEAVFQGALVRGGELHRQLRAQRVLRGWRGVAEDVRRAGKLKAQMQQRKAHKVLMDWRRLWLARVCCRRVYYRRVLVGWYERTQELRQARARLWHATHVILYGSMARCFTVWRQHTQAMAIKRAVFVRKQRALADALRRGDELAARRNAELTELAFRAWRWLAIIYKEVQRRQAVATARMAAVVWAGWRKYTRVRRAREAKKDTVLRRRLLSRPLAAWRKASAAAKAKLGAAVMHRRTVLLESAMCAWFSYHDHVAARRAALRRALAAGNAAAAAALRFRAWEAWCEIMMRRIAVGELVEQAIRRRCRRSLQLAFDLWLKYTQAMHSGGIDPGSPYLTPRRRDADRRLVIRMAAMAGNEQALVGSDSVPSGGVLDGLYPSSGLKHAMERQQEVAAFLSLARHTSPSAGGAGGGSRGGSGATAGAAFASAMLAERQRLRHLDRDRRPSHDSGNGPSGSGSGTASMAGSSHASSITSTLSSGANGADLADLALQVVQVAPGLSPQGKGKSRGPSSAGQPQYVTGTAVQRPLNTFRDSDTGVGGSPYDDRLDLRALYDVAGTRASNVPPPPAAHGYAGPQALFGGFTPGHLPVDKLTPLSRGTATFPNPSATVVQITPRRIEFTAGRSGTGAYDIAPSYYGYVTASRSQQVRRESDSDVSTTSSDLLPPAEVRTRAAATAAADDAITAAATAVAAVSSWRSGTASREPHSSPKGRSWPRGLAPQATGGKGRFGGGMVATMQPSSTGLQRPAAAAAVASGGGKGAGMLQLGACGHMSGWRTSGTSQGSAGGISGLHASGGGAGGMPSAAAAVAPAVPYRGSILDRRAVQQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBN1</accession>
    <name>A0ABQ5SBN1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037">
        <property type="entry name" value="FORMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037:SF24">
        <property type="entry name" value="POLYCOMB PROTEIN PCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        </source>
    </evidence>
    <sequence length="3603" mass="360984" checksum="02618A7532500560" modified="2025-10-08" version="1" fragment="single">EDDLVRLIQAIPPVSSKPPPPPLPVAASAAATATSSPTVLLVSAPVGCGRTALVTAAADRLVAAGVWTCAAFSDLRCATGPEEAALEMALALNVPFYAADRPAGVRLLSWLRTYGSQLRQGLVLDNVDVLLGVGRDEALAMARGGCTAATAAATSSAGCGTATATSAAPPASGRSSVENQPATVTDSCTADFDDPDLPFEDPSDSPLSPTEAMDRRRRVLRLLRDVVLAAPGLQLVIISGPGVTLELLQPCVPQDLSIRFQSLGLMSQGAARQLLRSALGLRNPSNGGLAGGRSSGGGGEGEDAAAALDGQIDAIVWACNCLPGLLRMVARAVRCDVHNLATLSSWAVDALRHGGEGGDEPSEAATSIFSTRLVSQSLLHLPPDARTVLNCLVNLGCPLEEEDVTAAAAFLGHPGSVRAGLCCLYDRGFLTFQDLNQRHDVVGHVRQWMVQYGGFMLGPARHGLSMAGAPPSAPPPGGKTTRGGTTRNRSAVGAATSTGGSAAAAINDSGHPQSVAGDAAGCSSSSVEAVPEPVSSRRATDAGHSLRCTASDRRSSAGARGSSSGGSNAAGNTGRHGGGAREKGIRLSVLVQHLDGLLSQVSELYAHGCRVAALRVFNNSRALADRALLLVPAWINRISPSSATAVAFATVMTRHLNNICRHVLLPGQLARVSLDLARLVMWLPSPHKRLTAYVTAARTHIHLMALQPAVQQLTAAEALAVQLYPALAALVEFNVAAAAQPRAKSTGGGGGGGGGGDNGSGGNAGGGLNGGSGGGRRAVTRVGSASVGGGGGGMAPGRFPLSPRQRPGISRVPPQVAQLILAHAELAAAQKRYDTATRHLEAFLELLAASGMAQSLEGAQARMQLGDLYLLRNRPPAEAMVQYEMAATLLMQLYGQSHFRVGRAMLAAARCSPENAPGSPVDGAAAAAAGHRPHIGRRTDGHSMSPAKSYRALEMQLKDCLYLQQLSQGKFNLDVAEVEVARGALMIGRNQPDDALSCGLVALGIVLEILGDGTPQVVECLALVALALRLKNKPAAAAAVIRQAHAVRIYAAMVAAAAYDATTAAAVIRPRPTQAVVLESLADAASSQLPLVPRVQAAAVAATGSAAAATTAPISADGRERPSVQNSADGSERSQKRHQRSPHTRGTPRTTRSHGGTTLLHRSPIIQVPLPDGMEPDGDDTAGSPRSTAPGRNPSSLLNPSPLLVLLSPEDGDFGTRDDSVNARVAGGSPRCLSDSPGWPSVQRIFSSRMVGANTVAASVSGGGANSSGGGDGGGNGGGGTLVHHVSYPPPLAPVDSAPVGFDETSPSPHAASSDAKWPMNWSPAASPSSRLDSGADRAAVAAATAWPSGRSEHFASATAASGGSAIMATPPKQVAPPGGIGPTTDCRKPVLQLAAVEEEGDESSLVKAGRVSSGSAMARLDSVVPPEMWADAPEEFAAAAGKEREAGSLTAIAGAEAEAVAPPTPTPSAPIGTAAAAAAPPPLPAEARDEALLTASRDIFLEGVGIQLPLSLPDPSAAVPSTVTGDSATTATTAVTATTATATDTAADNIDPWPTPQRARLLGSSHFPDPLHPSRPLPKRYTSPTVNSSSISDSAGGPYSPGLSLPQPPLHAAAAAVTSSFDVTAATLATAATLAAAVASSASAPSNSNMSTPPGGGYAAPSDATPRSGVPLAQMSWFAYSPTEGRTAQDSRALRGGIVFPTPTTTPGQSKLEHHKAPWLSWATSCPADSSPNTPRPRELRFSDPVDVPKSTSAGSGPLIGSAVAGGAAAAAATGPLDYTSGSAEASAEAQAGDFQEDWAVAAAAAAAATLSPIRSRFTVPVVSTVHMPSPPSPPQPPPQDQHQHEDAAAEAPEGRQDNGRGKYEGGEGGMGAELEVEAVGGKAVLTEAVGNCFVEPPSPRRHSTLASGHGDSTPDLPPPPPPPPPAAVSQSAMARATLYDLIGSAAAAATLDPTPLPTSSAADAAALTEIGVFKRTDLLVAMRAAERVEMAAEGVVGTSTSTPSPPPSPPPSPPPPPPPPPPLLDEALIPSRPGATAITGAAVAAVAEKRTDTVDALSASIADGVAAAAARPEGQQDRLNAVNEVPGCDGGGGDPHPEDLRNLRSEGRIAAQQEGAEVGEEEREKEWVEEEEQEEEEEEEEEEPSLPGSAGAGELAAASSPTRVGVAGGGSGALEAPPSEELLVGEGHSGVATPLSASAGVDVFVSPSPTNTGSPMGHSSMGHSPMGRSPMVQSTLDKHLVFTGGAAAPFPSTAWEQQPLPPPLLNRQQSQMAGGDCTKPDCSSTILAGGEGGYDTEAANVAAAAGNDDSDGISSRKRPHPGGVGSVVFGTAPAATLAVAATHNNPPIAAPGLLLPPSDSLPGCVGSSATYVPGGCSGGISAAAAATTGGADIAAAAVSSGGRATEPAASAAAAAVPAEAVKGPDSPFESTVGSSCVDGALYGIDYHPGSGVGHLRTATLSPSPAAPAPPSSPPAGQGATSCPPSASAPVAYLPHNAPSFERAITSFDAALVIPEASIVSVPGVTGAATRTTEDTTQHNQSQQYHHYHHNHHNHHNQQHNKDNQKQQHQQQQPLSGAAVTSGRQQQLPEAGFESPQESHSVMGMPPLGLTLPSLESGLTDCSPFLGLVAKSTAEGLMHDKDTADDRWEEGRSKTPGQRQGNQGTKQHDLHSAAAAAVMAAACQTAEIPNGTMAATSAFAEAAIAAKATTAVAEAKIASAFCSRDATSGEVGAKSSAGTAQISVTSSKALGAFGSMVTDGDGGHLGGSGTRPRSESGNVLVAAPAVVVAAAAAARAGASDPAQVREDDKQVDEDRGMGMEAVAGAAWQSATASRASSPKGRTSGGGAAPQLFAAPLSSAAHIMASDRAPAAPLSPKRRVISGGGSVGSGGGGGASHPSGFSLPPRSRVSSGGTSASTTLTSSPKSRFRSWAAPASPTSPRTRLAGTGAGAGVGVSASPILRKSRTYSGGYAPRSSSTASPKSTSTSPRDRTRSGRTTATAVQRHVPSYSFFRTADVDSDPKTPGAPRPPSGSAGKPNGSGESCRIPLPPSGNGSGQRADRSSSRIPTPTQLGGTVTTAMTAPPPSPPPRPPRHRWTVAAVAPAGMIVSGSSSSGGGGPGLDVQDPAAAAAWTLYSSPAYGIHDTVSSPPRADADATDTHDGAVTSPAARYAGSYTAVYLMRAKAKSIAMAAWGMGSGKSDGSGGGTDGGGAAAAAGGSGGGGGGGGSSIVQQSSVDVGLLAAGAAAAGTPCDPRDGVKMHNPLFVKSSTRSLGGDVTGSSYGFEQDDEEAAGMLMHSGDVLVGVRSGSDGGTASDSRRGLEGRINGGRNRTISGSGRPSGIDSRTTRTNSISGTMPPALGVSGRLGSGSGGGSSVLLGGGGGGGGIRISSDGSFGAGRNSTGSGNGNGTGTGPQSPHGGYSTSVSRRTTATVGGRSTGSGGTTGGANYTGGRGSSRISMAGSSGSYGAMHSNSTVGGSGSGTPFLARNVSRRSGGGSGVSRPRNGVGSHGSSSRTSVGAASPQLPRNSNNRNSSRIGSYSAIHRRITITTAVPSLPSAAAAAGITTSYVLPPQPQRQPQQQQQQQQQQQQPQNSLPPRRGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC08</accession>
    <name>A0ABQ5SC08_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Fibrocystin-L</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_011397</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055401">
        <property type="entry name" value="CEMIP_beta-hel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052387">
        <property type="entry name" value="Fibrocystin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019316">
        <property type="entry name" value="G8_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46769:SF2">
        <property type="entry name" value="FIBROCYSTIN-L ISOFORM 2 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46769">
        <property type="entry name" value="POLYCYSTIC KIDNEY AND HEPATIC DISEASE 1 (AUTOSOMAL RECESSIVE)-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24606">
        <property type="entry name" value="CEMIP_beta-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10162">
        <property type="entry name" value="G8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM01225">
        <property type="entry name" value="G8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56988">
        <property type="entry name" value="Anthrax protective antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51484">
        <property type="entry name" value="G8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PA14" evidence="12">
        <location>
            <begin position="1439"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="domain" description="G8" evidence="11">
        <location>
            <begin position="2206"/>
            <end position="2326"/>
        </location>
    </feature>
    <feature type="domain" description="G8" evidence="11">
        <location>
            <begin position="3075"/>
            <end position="3205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="4053"/>
            <end position="4079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="4119"/>
            <end position="4212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="4062"/>
            <end position="4077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="4125"/>
            <end position="4158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="4159"/>
            <end position="4170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="4172"/>
            <end position="4181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="4189"/>
            <end position="4212"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="4212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI67179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4212" mass="450137" checksum="29D5867D55EA81EC" modified="2025-10-08" version="1" fragment="single">[encoded data, 4212 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC18</accession>
    <name>A0ABQ5SC18_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011506</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18013" checksum="9D174543CC4616AA" modified="2025-10-08" version="1">MGGLEFVGRVVSNRMQKTVVVAVSYVVWIPKYKVYQKRIARHKARDESQSLVIGDIVRIRKSRKYSKEVSYAVVETLRRASVYDSTAATAMVTALDAARETDWVQVAQRRLSEADVRLMKLREMYEREIGSGISLSGGVLAVSNSGLGGTGSLGAGASGAAGERGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCB1</accession>
    <name>A0ABQ5SCB1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024224">
        <property type="entry name" value="DENND6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677">
        <property type="entry name" value="LD41638P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677:SF0">
        <property type="entry name" value="LD41638P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="848"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="366"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="852"/>
            <end position="867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1001" mass="104691" checksum="C376DDDCD7A3E587" modified="2025-10-08" version="1">MNLIPSSHQWIVGICSIIFDIDVGQRIEHLVPEGCLSKEEQHDVAFHSFPDSMSMELHARTSIKDSTFFFRARRRGKLPNGNLAPPPVAESGDGESGSGTDGSAASSPGTIAKAHADSDNAKHGEEQTNSSEGDERSKEERFLYGFVFCRQRQDASLRRGGEQISVVVLAEHPLSSVLQPLSVVAGHQYFGSPGKGALQQVYDEVCRWPCPVAGAQLQLPVAFTPLTARLPAWNTLPHPSTLTAPEQYGSNTLPFLRSTSSRTVPLPAGPSLGGTSYRRIPSQTDDGAAHVLVGAPGIRTSGGMSGALSTASSAASGLLSPSRSAPVLPPLGSPAATAAALAASPFLSSSGVVQAAQIAVAPAQQHLERAGSSGDGADSTGLANQRMSSSAEVSLSPSGIAPSSSGGGGGANSAGNLSLSGTNSSTARGEVAATRSLRQISGLLAEGGCAPAAVPIYPVVAVVGPGGGPAPAHQRSRSSVQVPSRSSSYAGALDDVAQTTHNSNGGGRTTQGQHELPEDQGGSRGGASQGYRRTPSTSQIGAVDGAAIHGAFHEVDVYTPLSAHLPRLWQLWEMTLLGRALLLIAPTPGETSAGVAALLSLVAPLPYAADFRPYYCIHDTAFSRMASGVLPGPEARDVPTLLGVTNLYFIRALPHWPNVLSIGKREGAVAAVQPSGSLAASISRANAAVQALRQRTQGASVLMSGHMEALWSTYKPLCRPDQALLQKLMLPKPGDVKSKVARIALVNSDLIRRHFAELTTAFLAPFSRYFEPDPANDGRVPGWNSEEFLFGLRSGGVPLPQPLLDRVGSPSAAVELYARFTSCLNFAAWFAARRDHVAHLIAPSWPRQRGTSFREGDHGSANGRDDSASGSAGTVRGKAMAVMPEGGDEDEEGGIASWFSSAAQHLDEVKLIGLFFSVEQQLMEAQVEAASPDAPDEAPATVSRLQREMTMLFFGGRMPEELQLTCVSSPARRALLASLPLTAGQAARMQQLLDMLQVGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCD8</accession>
    <name>A0ABQ5SCD8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
            <ecNumber evidence="2 6">3.2.2.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_011129</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>9-ribosyl-trans-zeatin 5'-phosphate + H2O = trans-zeatin + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:48564"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16522"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:87947"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>N(6)-(dimethylallyl)adenosine 5'-phosphate + H2O = N(6)-dimethylallyladenine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:48560"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17660"/>
            <dbReference type="ChEBI" id="CHEBI:57526"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the LOG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.n1" evidence="2 6"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00730">
        <property type="entry name" value="Rossman fold protein, TIGR00730 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31223">
        <property type="entry name" value="LOG FAMILY PROTEIN YJL055W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31223:SF70">
        <property type="entry name" value="LOG FAMILY PROTEIN YJL055W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102405">
        <property type="entry name" value="MCP/YpsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="285" mass="30586" checksum="6F1E5D0E41B4315E" modified="2025-10-08" version="1">MRSSCVTDRNILLSVDRKQNCCGASSRVSRIQKLCELKTPNLCPVSTRSSGLGLIPTPTVGSQIMASRELRKICVFCGASTGSDPVYMNSAKQLGEYFVRENIGLVYGGGTVGLMGEIARTVQGGLGDEGVMGVLPEALAPREVSGALIGKTHIVADMHTRKAMMAQHADGFIAMPGGFGTLEEFLEVMTWQQLGFHAKPVALFNVNGFFDPLLTFFQHAVHEGFVRAHHHTVIISADPEELVQKMRLFQPPVSLVAAALNSTVPVGLDVSVSSRQAEQADSSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCJ0</accession>
    <name>A0ABQ5SCJ0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011114</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF495">
        <property type="entry name" value="HELICASE SENATAXIN ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="3">
        <location>
            <begin position="580"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="3">
        <location>
            <begin position="799"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="3">
        <location>
            <begin position="1105"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="4">
        <location>
            <begin position="1158"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="748"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="907"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1517"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1726"/>
            <end position="1753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1784"/>
            <end position="2032"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="997"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="908"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="932"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="942"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="959"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="978"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1518"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1563"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1785"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1797"/>
            <end position="1829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1847"/>
            <end position="1856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1918"/>
            <end position="1969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1976"/>
            <end position="2032"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2032" mass="211554" checksum="12BCB438B7D9DB43" modified="2025-10-08" version="1" fragment="single">TPRGGNANNPFAVPLHDRRLLEPQPDVVNAANAAASSTASAQSARISPPAVSWAAAPADTGKAAVNKVDLLKSIEAAVWEAALAARSGAGQPSTRAIGAHPVKKPRRTALEPGGGSGAGEPVKGRARPVNTGKADKARKELLGLLSRGDGGKRSEGGGGDEISGRGGLGNGTRSRGWAVSGGNGRIGGRTAGVMPAPDPLLQQPMPSLRAQHQQQRAAEAAATAEAAGTAPSASLEAASKGGGVSTTDPAPAQTGTGFTVMDDGFFSQSPCNGETPAGATAGPQKQRVGLGLLATVGLTAATRARGVVHLEAAGLRGVGNKAKALPPLQAVLDMNQVVRDVIQLPMGALLGLDGRGGARRDLPLTTSKAALARGTVPVHFTSLEQYTDIFRALLMEELSASVRSSVEELAAGGAPPSGVQGIVTSVARTDDVHSLTFRAEECGLTTDRRNMCRPDDLLLVTSKPLKDAKAVAQPPGPCVLAVVREVTKEGGCSMVTVQACLNQAYGGIGGLMLPSSKWNLIVLHSLVPQFREFTALCYLYRVHEPMVRFLLNPKPPAPPATSWDSSGLPPALRAGLAATFNDSQRKAILSAMDDSFFTLVQGPPGTGKTSAIMGIISVLLVRHELSGSSAISTVAGSCSYETAAQATTVAPGGGSRNGKLLAAAAAAFGGKRVVGRSTTTAGPAPAPAGPLKKSSAAKRFEADMAAKAVAATATGSEPLKDRSDAAAALHGTVAREMSVPMTAIHQRGSASDRLAPSALQGNDICGGGGDGVSTGPASGRQGVPAVPQEALDAFDFATQPRCRILVCAQSNAAIDELLMRLALEGVWRADGRRKPPAVVRLGRAEVVHGSVHALHVDSLAEQLAGRHRGTVEASSNLEKLCREVSDARREYDEAKAELAQLRLKLTEQESLQRQPAQQARNSSEGDDGNGEDGSSGSYSDGSDGDDMDLCDPEAEMDGPSDRRRVQREMRQKSQVGETGCGGGTDGVHGDGGSNENADALKAQVNTLELRVRHLRSTMEATNRNKLRSSRDLERQRKEVRQAVLLAAEVVVTTLSSSGGDLASLCSTVVTVATTAATASVTGAALGRSGRSAGEAAPTLAPGAYRFDALIIDEAAQALEPAALIPLQLLRPGAKVVLVGDPKQLPPTVLSRAADAAQLSQSLFERLQRAGCHVCLLSRQYRMHPDISRFPSSFFYDGALQDGETITATSRSAPCHNHPLFKPLMVYNCAGQMEQGGSNGGGNKGGSLLNRAEAILVAELCTGLLVHFPDYRPHVAILTPYRAQLSVLRSTLQASLRKARLREDVASILARIDIVTVDSYQGREADIVIFSTVRAGGTSSLGFLTDVRRMNVALTRARRCLWIVGHAETLATSPPWLMLLRHVATQKSLVSVRPPVSSLLKLPPDSIEPYRISEAALDRMLEGGSAGSGGNRSRMAAPATATATAAAATAAGPVTEPMAMAECSRSKRPGNPISLVQVSETNPLSAPVMTGRAVPGPGPGPAVAADRPRFGVSRLVPANKDHSSVPHHSDKLRFGSGHVGGSGCAPPEAATAVSNIEEVRAGSSGNGSGSGSGKGVGKRRAEGSAEQTSGGADAPPDRPRKHFAIDVNDAPVRTDTAITTGAGRRQAIDGAAETDPNRSGAVHSRNQAAAAAAAAGGDALRGSAAPICQRPQLLPQSTDARHVDVDSTIGVRALAGNTSTNPGGDVHVHARMEFANPPPALATVTETTAGQAGPDAVPRSGRSAPEHPLQATSPGECAVAVRACSTAAKGVQHIRLPPRISVTNRASAAAPVHEAAPLQPPPPPTLSPPSLPPPLPTSPPPPPPPPPPLPLSTLISSLPPLPPPPLSCSPQMPSSQPHRPTAPGATGLPAVSRQDNPLSGVERGADCSRRQNTTHSYSAERVGRNEGRRQPQPLPGTASRHEQDTMVRDSRDKERNRPVDGVEGYRREKSASRADVQGRTRDHDVAGGRDKSRRSRDRPDELREHGSDCNREHNKIRYGEQERVRLPETHRMHEQERYKRSKGDRDRDRDSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDA9</accession>
    <name>A0ABQ5SDA9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CW-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_011374</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042778">
        <property type="entry name" value="ZCWPW1/ZCWPW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011124">
        <property type="entry name" value="Znf_CW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15999">
        <property type="entry name" value="ZINC FINGER CW-TYPE PWWP DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15999:SF2">
        <property type="entry name" value="ZINC FINGER CW-TYPE PWWP DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07496">
        <property type="entry name" value="zf-CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51050">
        <property type="entry name" value="ZF_CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CW-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="338"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="474"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="383"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="434"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="510"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="531"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="568"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="594"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="653"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="678"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="689"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="743"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="781"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="834"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="853"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="908"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="923"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="970"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1014"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI67161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1050" mass="108578" checksum="665C36E1855A642A" modified="2025-10-08" version="1">MPRGRKKKEEQALDIQIHEDVWAQCDGCQKWRRLPPGTSLEESQTWYCNLNPDKKYNSCSLPEEDWQNSGDKEISAALGSELKAEAAKLALKKAAGHDIGAGTGSGRGRKRQTDNDASWGLLPKKMRTLEVETPTATSAMAPAAVVSHAPIATSVPQMTPQVPVATAPAAGNMTFPSLQDELKALLNGCTRILNNGGTWEPTRSAGHMSQEHVIIPQWFWAGMQHLVPRAAALATAAVEVLTALQYSASASQQSATTANSIQQAELYVRLSLMSAASMLLPVVKLAMQGVDLAAAVATATKPALPQPAQSLAVTPAPAAASTPSAVAIQTPTPLPAAVGGLFSAPSQTPPPGRAAPDTVSMSMSGGGGQAGRSLPPRPPMPLPAAVAVTGTSAPAPAPAPAATSPRVAPAATSPSVPQPSATSPPAAHATTSPPAAPLPPPPAAAAASTLSASQSPKAIISAATTNVPGATAVTSGGSGGGVAPVAPVTVGGGASSQRAGVSAAGSLSRASSPGTTSTAPTDTGPGSSGRGESRERREPCDPFDSREAREPRAPRERQRDPAYERHLQGQQQQQQVAQQPHQAPPSHQGTGRPGGERDRERERGADRERERERERDRRDGPLGRDREYHRDYSREREGSTYPPHSSSHHSHAHYREQRERERAGPGSPRQPPHHGYGADSGGGGGGSGRDWHDGRGERRDSRERERTYDRQGPPPQYGYSGGSGGAGRGHREGPHGHHGFGYRGDRDRDYYGHEGGSRGGEREGYYRGGSGVPRYGGYPPDAHRSDRDRGRGGGSGSQDWGGRERDWDPAYDGNGSYYEGGTGPAHYGSGPSGGGPPPSQRAPPPPPYGGDPYGQSGSYSSQHHGYPHQGPTPQLGASGHLSSPNRDRGPTSRAPPSTYQPSPHHTPHPPGVGQPPPTLGLNTATAAAAATTSGARPVPLPPSLASLPPETVAKLLQDQGHTVKSVHVVSPTQAPTAGPSSRTGAGSAPSPYTSGYQPQGTGTSAGSGQPMTVAQIRAAAQKQQQAAGSSQGYVHGSGGTRGPSAFGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDF6</accession>
    <name>A0ABQ5SDF6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_011837</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="5">
        <location>
            <begin position="111"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="5">
        <location>
            <begin position="309"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="195"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="276"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI67676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="577" mass="60198" checksum="ADC47F10BA17F509" modified="2025-10-08" version="1">MALPSTTEELLALLDRNRWSTVAFRRRLDIADWPWELQLQYQTPSRPQQLKPNLQQQQQQELQQAAELTEPPQPGTATTIASSSHLERAVQVNIGDVVAREAAVAYGKIHEHVRHTPLEPSPWLSSLAGSASGRSCVALLKLESEQHTGSFKVRGALSKLMSLPPEARSRGVFTASTGNHALAVVYACKSLSEQSPHPAQIDDAPRAAASNMAGGRPSVTWDIEAQPAVGSGPGAAPVPAPGAPVQQPQLSLHKGPEGARNRVNGEATAAASLYSAAANATSDPERDDARGEQDSSTPGLEAGASDSSAFGGLRPQLYIPTTASPYKIRKLMSLGAELHLYGTDCLEAELAARSAAKKADAVYVSPYNDPWVMAGQGTIGLELLATRRRGQLDVVLVPVGGGGLIAGIASVLKAADPAIQVIGCQPAVSDVMARSAASGTIEPELADTHDGREVTLSDATAGGVESGSVTLQPCSNFVDEWVTVSEAEIAEALVGLLEEQSKLVEGAAACALAAFKRLAPRFAGKCVVVVCCGGNISVASLLRAIELAKPAAMGTTKVTGISRNARAMAFSGSNDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDS7</accession>
    <name>A0ABQ5SDS7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin protein ligase</fullName>
            <ecNumber evidence="14">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_011881</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the BRE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="14"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16499">
        <property type="entry name" value="RING-HC_Bre1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013956">
        <property type="entry name" value="E3_ubiquit_lig_Bre1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163">
        <property type="entry name" value="RING FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11 14 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="824"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="10"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="174"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="311"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="424"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="602"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI67633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="877" mass="97043" checksum="F74FF5587712B818" modified="2025-10-08" version="1">MDSKSTEQKLKVLSHQNQQLAVQLEEKRKASKALEDKVAAYEQKEQEYEQTLLCVNRLWSQFTSAISHLCSSLAGHVDLRPPATPQGAVAMRDPFLQRLVSDSAQAKPFVDFQKRVDLELTDVEHALQERARGCKDALAAVLDAVRSIHARQEDLSAKMCEIATAPASSPSGLQQLLQAENVRLDSEAAALRRELDAVRAQQMCTSEELRLAEDRRIEAEERIRQLQNELADTEQELSNVQKKYFTLKNSGGGAAGGGAGGAAAVAASGGGGEAVAASSGGPSRQGSLLSALPSGAAASVVEGSSDMLDEVAELQALLAKRTADLEREREAHLRTRRELQEVQARVSDEAWVPSTRLYSMAQQQLAQLNEALVVRVRDAEALVRERDEALREAATKASLAASEAHLRSKLAGLERSYRELQFGKADADRARAEAEATLQRERARGGNTKTVSELQALVGSLQSQIASLQSQAKLNKLAHERLDASAREVVEATSKLSARDTELQRLRDALGRRDIEAEEARRRELALKEAITDLRAFVDVLTTYGNDPREVVEVRASEASLRTQVAELKQQLQGHPLHATIREVTAAEREARGQLDLVAVEADELRLQLSKGQRQAAEVEAQLAHSRSECELYKQEIEVTVNAFEELQAQNARLVAALAERDEANNAVVAERLKLSQQVPMLTEAADAARGEAERLQREVSELGIVREALERDMTRLATEMAQVKEQLRANVTRLENAGLEVRQKSEAVLALQQQLEAANRQMEFKRQALEEADQKHVKEKSKRQRIEEEVKVKVDRLKKLQSPAGAARELQEEVDAMRRLLNCNVCHERQKNRIITKCCHVFCDVCIDRTLTARNRKCPGCGIVFAKGDIKSFFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDT1</accession>
    <name>A0ABQ5SDT1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012374</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="535" mass="57230" checksum="CD21FF5AF473DEFA" modified="2025-10-08" version="1" fragment="single">MTEVPNIFLVPIYMLRVFSFYTLPMGLSANCLHHLAISVVVMSVPVWLFCVFGSSMTRVQIIAAIAAAVIFPKLARSLTTKLRSLRFWWQPNLCHTRWMQPTPEAPEAIQAILKLTRKGRVNCTQGRNHASASHDPSVNGISRTSPSSIATAGAVVDPLKSSYFTGSATTNRYPECRTTGTILQNTAAAIATYASGPETKSRNEFGSKSTPLQASLQPSTSPFPTPAEAARNKALGSPRVFEARFSGSSLKAPVGLSRCTIVEDAPFPPPNTQVVMELHEKTLRVGGRRLRYRGYRPADRTLPLLLQAPVGPPGLFTAGQSPFVDVRGGRNVHYCYSHTQNLLAYGPGTTCSTRNPPYPPTQSDPHQPDVLPASLVANCAGMAVGTGPPAPAATVARSSRPLPASPHLTAPYPSGATATPSHVNSYEPPIHMHPQNNLNRHNHHNYQDSRHSLSLSIKVPWAQPSDLPAGGGGLLESLNEALASGSGYMVVGAVVRPGCILLQVDLMAASETGPDLLRGVDGVTTEVRRRCGPDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE54</accession>
    <name>A0ABQ5SE54_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012493</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="543" mass="58725" checksum="02580C66AC4629CA" modified="2025-10-08" version="1" fragment="single">EIKSQGIDILIKQFCGLFLFVHLVTVAVMTFSICSQPPADTTEFCVAHKVCSDSRCPPSASPCDRCRRSCNILFGSWGEHELDSLLKYGSSCMGDISVSIQLVNKQAPDRLDRCFTAARDSSGCNDSDGDSHVPYHEWILCRPHVKLPPGLPRPPRQPPAFSPCSAFTSEDDLEDDCEEWYNRNVSKEKYPDWLREPQQGTSDASPPQPPSSHLDGCSNSNLDPDDRWSNRNVAAGFFASWLASATDGPQALEEQFCQQLFRKATEFVTVDSDMLESSIGIPTVEYAHTAAAIRPAAADACNPFSCFSTDIPDSSVSRGNSDDDGFCCEALSSPIGSDCRTAATSDDDSRGSSRSCSSLNLGSLDLGSGGGYSSCDDEEEEEAGRAVGMEKDEQLLRPGFEGSSRCCDVGLNQSPCKPVDTSLDVSTPTLEGDTGDVSLVRLVECTSWELEIEAQQLRLALLDVDDGYEDLVLWDGLRQCAGGAAAWGCGVLEGCSWEEYAETTVSALQSPWIKTQLDGLLDDVVAGAMFGNGSEDEDEELLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SES2</accession>
    <name>A0ABQ5SES2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047011669" evidence="2">
        <location>
            <begin position="22"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="419" mass="43347" checksum="26B8D91B5EFFC855" modified="2025-10-08" version="1" fragment="single">MAQKVLLSQALWLISIPEAAARLHGQHARGRISISEMSLRSPKSSSGATTPGMLVGRLLTEAIVFARNAITRFANSERPNAAALNPLAFSESLIQSQQDVLPSLSSFQRSLKAATETTEPADSYELDPTDFVDSIDEVAALAGIAACHILLSIGHSDPRDSASSASDALRGLKETLRAATTALGEGGSGSGVAILQELGLGSAPFQVCLLVVLAHNLDRLRQIAFSQMAVCRRDAVAVVKLLDELTSLAYGSRSDCAIAAGGGGGGGLSGRLSIVVQLSDREHVSRKASPSGAAAVADGDEGHGPFLQALREIAGELRSSMSASGNGSAAAAVLETVLQAAYRRTIEELQRTGVSLSPGSEDGIPDKGTASIDNRHLSTSMVQLSRVDSWRSSFSSSGGGAGGGAFNSNAQQEMPCVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEZ3</accession>
    <name>A0ABQ5SEZ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="6"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="275" mass="28937" checksum="E97706C049FA34CB" modified="2025-10-08" version="1" fragment="single">MARPQKALVVDDLAVNRLIIGTILQKLGFEVLEAENGLQCIDVYNQERTHLVCVFLDLQMPVADGWAATKGIRELESRTPDLCHLPVPVVVCTASCLDDLVDNGQTVAQRAVSLGANGAVRKPFTVCAVQRILDEYAPQWRAAISQDVLEAPPPASPNRLSTLSATAFGLPTAVATPISLSSGPSCQAHKSYPFWSSGHCQSPPPLMQHHTLFTSVPCMTAVAAAATAFDTAASDVPSNASAGCSHSALSSAWCGRGTCDVGTCRGGGTREQEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFL0</accession>
    <name>A0ABQ5SFL0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CCDC113/CCDC96 coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_013202</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051885">
        <property type="entry name" value="CC_CF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025254">
        <property type="entry name" value="CCDC113/CCDC96_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15654">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 113-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15654:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13870">
        <property type="entry name" value="CCDC113_CCDC96_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC113/CCDC96 coiled-coil" evidence="6">
        <location>
            <begin position="295"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="286"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI68717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="515" mass="57068" checksum="B07C3AEDE34E9F60" modified="2025-10-08" version="1">MADVDDAQGDQYGDDGGAGSEDARGATADSMGADGQGLDAFEDGQGDYDASPLGTPLQGARRGSAAAGGTGGGGGGDSDDDMPMPGRLEALSQRSRSADSSSQRYSRPGSAVINLRGSRPNSAAIAQEIEGFEDDFMERVEEEPEEEMMEEEEEEEDLGPSIEELIARAQQEQARLMEANDALQRRARMALDFRNKGRPPVNRDLSRLDGAATRYRAALRQWIEILEERDSVESHYQTTIFDMKSELEERIKRADDISKAYKHFRLEVAKSAEHSKTSRPISEKLLAQMESEDAAKEEEVQRVRLKNIHLTNQLRRIEQTLRQKEELAEGLHLIDFEQLKIENQSLNEKIEERNEELLKLKKKTTTTVQILTHVREKLQFIEKENAALASQLSALEAELADKRDRLGRAKAERDTLRAKGRKIKESGSNITSTQLLDDIEIQKEKRETLAAAIEEAQRQYAELSDSIQRTNTRIMTVAEELQQAQAAAAIIAPGMGAQAGRTATGAGTRSITQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFL9</accession>
    <name>A0ABQ5SFL9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="220" mass="24126" checksum="5EE27F50CF41A716" modified="2025-10-08" version="1" fragment="single">REERECRFCSTVLPPWQETLLPKRVKPAPEAIVNVHAPDGSLHRIPLRPGLAGRSRFLRSVRQALSLPAHAQLEMSFEVAVPAIEGCTRPTLINTRDFSVASHIATVNAGVRRAKWEATGAGPEDAPGREGEMEGAHVGQEAERGPGQQPAGGEGTATQSAPTPFAGQPGQEGARQDDREEEERDYERGQRKHKRWPAQQERGVSAGPSTRRGITRRTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFR9</accession>
    <name>A0ABQ5SFR9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoenolpyruvate carboxylase</fullName>
            <ecNumber evidence="4">4.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_012369</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>oxaloacetate + phosphate = phosphoenolpyruvate + hydrogencarbonate</text>
            <dbReference type="Rhea" id="RHEA:28370"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PEPCase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.31" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.90">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00595">
        <property type="entry name" value="PEPcase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021135">
        <property type="entry name" value="PEP_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022805">
        <property type="entry name" value="PEP_COase_bac/pln-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018129">
        <property type="entry name" value="PEP_COase_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033129">
        <property type="entry name" value="PEPCASE_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000584">
        <property type="entry name" value="PRK00009.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30523">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30523:SF33">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00311">
        <property type="entry name" value="PEPcase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00150">
        <property type="entry name" value="PEPCARBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00781">
        <property type="entry name" value="PEPCASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00393">
        <property type="entry name" value="PEPCASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI68046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="967" mass="108950" checksum="400E265F34B31F22" modified="2025-10-08" version="1">MNFRVSSERRAGPGQFLNVLRDDDSLLRQIFFDILRHHHPNLASKVDVIYALSQAWCTSQSDSDFELLVKYITDLLPEERILVASSFSHMLNLHNLTEEVNSSQIGRAVRLGEMDSPTRDTNHSLLKLTTVNGLTTRQVYESLCNQTVELVLTAHPTQALRASLLKKYAIVRRELDTLRSKRMSQHEKIETMQAIRAAVQAAWRTDEIRRSKPTPQDEMRSGLSYFSTVIFDVVPVFHRHVDTALEIMGLPRLPLDKTLFKFGSWMGGDRDGNPNVTAEITRDVVVLARLEAVNVYFRQVENLMFDLSIWRCSSEMKELAERIAAAESREAARVAEERKKRNYADFWAPIPATEPFRVVLSHMRDRLYNTRQVLHQCLIHPNMPVRGALEEAGAYLDIEDMARPLQLMYDSLIMTCDQSVANARLLDLLRQIRTFGLSMMGLDIRQESTRHTEVIDAITSYLGLGSYASWDEEKRLKFLTDELQGKRPLMPPGMDMNPDVKEVVATFRMLSELPHDSLGAYIISMAKTASDVLAVVLLQRETGVRPALRVVPLFETLEDLNNAPATMTTLLSNDWYRSHINGLHECMIGYSDSGKDAGRLAAAWALYETQEKLVSVAAKWGIRLVLFHGRGGTVGRGGGPTHMAIRSQPSGTINGHLRVTVQGEIIEQQFGEKEVCFRTLDLYTSAVLEAALDPPPAPRQEWRDLMSLLATDSCDMYRSVVYRTPEFYDYFMQSTAASELGRLNIGSRPSSRKQGGIETLRAIPWIFAWTQQRLHLPVWLGIGEALEKAIGTGYQPVLQDMYANWPFFTSTLDLVEMVLAKADVRLSAFYERTLVDPELAPLGVRLRSLLAKTQALILIVVRKNALLEGNTPSQMSTPNLDEKIRLRSPYVAPLNILQALSLKGLRKFRELGQLGVEYNPSDPEIIDLLSRDPHKKGEGAQHPFVSAMDDCLMITIKGIAAGMQNTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG28</accession>
    <name>A0ABQ5SG28_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35100:SF1">
        <property type="entry name" value="F15H11.13 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35100">
        <property type="entry name" value="FOLD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="321" mass="34634" checksum="02A08F95F4AE2E2E" modified="2025-10-08" version="1">MVSSAATAQVQALDLVHLPSFSSLLESIASSNLAWTKHDGIWFLKREESRNIRDTESRGSSAPGRETIASTCWKAGLRASTLRAVDLKASLLGVVSVGGLIATATQAALSPRGPGTCDRNMCERCCNITTSIPYVMCGCHAIRHRCTASGKTWGASLIGVGIASAVFHGSYGSFREWGRRMDFWTISAASNLMTRSLFPCVPASVTAAGILATPFKPFLVSFVNTTAMELKFLAAARRNPKLRGPQRLHAVCCLLGLGAFALEDWKPDLPLVHSVWHLLSSTAVATINHLLADVEEEERCKQGRSGQQRPQLALQMQPLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG54</accession>
    <name>A0ABQ5SG54_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013366</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="519" mass="56921" checksum="AB6DF2A476C77EE9" modified="2025-10-08" version="1">MPSVVGYGTLRPGGNPDACNPSLSTGTWKAFTGPSSRAFSMLRTKRYAMTSKHDAKGAKLQCNGMDPVCGQKGVQINARHIHEECRLAISTAVPRDTSKALSAWKRQEFDAASSGVLSNNPSPSMRVTRRVTGGLLLAASAAILGSYGAPAPFCGVAFAQTQPRLQLPPCSGRLNGQLAAALTRAVYSSVVRLQVMDEADFQVENFKLREREYKYYVDANFKQLPRIPDLADNTGGLSNSAYFNFIQYCLWKVVYRHISTQQDREAFARAVGAEFLANLGSMPEQWEQTGGFQADARTPEAVICAAVLPFLEVLRAGGYICRYQLVLGSHPGTWPQDWMTSQPAVMYADAGEAGEVGEAGLPNSGGEFLFQLKLHRPADILSNVALRSEEDGAWPRTVSCCLMQLLEQRGLCSGTVEPTSGAAEDQQQQCGSSSANNVIMPLSAGAASRNGTSFSTRIGGWVPRASRTRCCYFLEIRYSRWLWTSSPRRWYRIGVYGSRRRRRQTRRDDDRAANSKFAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGN7</accession>
    <name>A0ABQ5SGN7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_012748</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130">
        <property type="entry name" value="CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130:SF171">
        <property type="entry name" value="OS01G0895300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="9">
        <location>
            <begin position="314"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="536"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI68346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="584" mass="63674" checksum="9B449A9159A969A1" modified="2025-10-08" version="1">MTCDDVQGGKVEECTLPASWDPGESSWSHMSTSILWALRHTLRYALNHSHQSISTCIRWVQRLPPAISVIQPSLAEQPTLLSPSLQASKITSYMIERVGVPLQTQHPSGNLGGCSIPSQDSGTFKQTYFRSLYNQQLQASPEDLCARSWLGYQCTQLVCGRIRIHWSLGGDPPSGDGCAYQQNETQLLHMALQTDMTQYVALSWPQKTGRMAPADAVIGFLVSSSGNYNIGAFKITGESASEVQPDEAVTITNKRVVANKTALTICFTRDITQVGHGAWVNISVQSSLGMNFVASIHEFGKLHSLNPDYKCGADVKFVRPVGGPDDGLWRPSNVSGDSSDDVLAERLFYMRAHGALQFTGWIALVPLGILAARHRWIFAPVSIVGLWFQVHRTVQVVAVVLITAGFVLPWTSFDSNDEQQVLGVDHETRMSSDPLLSSHETLAITLMVVLGLHIIVAIIRPKPEAPRRRIWNMVHWWTGRGLALIAGVNVVIGITLWRRVSGGNGVEWIVPLVVLVTGWLGLTSWLERRAPTGLGREGSYAPPTGDGSALFTGVLPSSPTPSEVPSPKGGVPLLPLTRTQTSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGR7</accession>
    <name>A0ABQ5SGR7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="185" mass="19422" checksum="9BC937E5B72B970A" modified="2025-10-08" version="1" fragment="single">CAYTLQTVRLTGRKRKPGVSPHVNASAASPPPLTPRVAMAAAGATAAPPGCLDVDMDTGGWSFVGGATLHCPGGAAGRKSGRHENWLLSDGADGGITYLPVLYDDEEHSAVLLPLADPPVNGGGGGERTTRPPGAEQAPVWFRYEYESRGGRRGQNFWDKGLPEHLEQAIRDVAGEEALRVARGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH68</accession>
    <name>A0ABQ5SH68_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDB1- and CUL4-associated factor 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">WD repeat and SOF domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_013806</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat DCAF13/WDSOF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007287">
        <property type="entry name" value="Sof1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051733">
        <property type="entry name" value="WD_repeat_DCAF13/WDSOF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851:SF0">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA U3 SNORNA ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04158">
        <property type="entry name" value="Sof1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="111"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="375"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Sof1-like protein" evidence="10">
        <location>
            <begin position="408"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI69271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="499" mass="56426" checksum="D311366BFDD1C468" modified="2025-10-08" version="1" fragment="single">TTLISQDRGILLQISAVLQEALFFPVNVAPSEGGGPLFVSWRRRVTKEITMKVKLITRSEEDYTRERTSDLRKVQRNYDPLLHPLEKAKEYTRALNAAKLDRIFAKPFLAALQHDDGVTCLARNPKLVNSIVAGSADGDIRIWDIPRQRTLRRLVGHTGAVKGISFTPDGQTCVSASTDATVKLWKVPYAPFEAGVVQDDAEAVFEFHGKNAFRGIDHHWDRSSFATAGVTVDIWDHSRSEPIQTLTWGSDTVTSVRFNPAEPDVFASTGSDRAITLYDLRRATPLRKVVMQTRSNALAWNPMEPFNFTAANEDCCLYTFDMRKLESALSVHKDFVSAVMDVDYSPTGREFVAGSYDRSLRIFSVSGGHSREVYTTKRMQRVFAVRFSGDATYVFSGSDDMNVRCWKAKASEQLGIRLPREKHKQAYNDALLERYKHMPEVKRIVRHRHLPAAIYKAAKTRRTIVESDKRKLQRRIEHSAPGSIVVPSERKKKILAQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH77</accession>
    <name>A0ABQ5SH77_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase subunit beta</fullName>
            <ecNumber evidence="14">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_013019</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="14"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18110">
        <property type="entry name" value="ATP-synt_F1_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF82">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0139">CF(1)</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="207"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI68556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="606" mass="65136" checksum="1367DB2B95E4651C" modified="2025-10-08" version="1" fragment="single">AGLTNNFGYSYSFLPTNSGLCVGRARMLSGTTRFAATCLRQAAQGRSTALLNAIRAFAAEPAAAASADVGFVSQVIGPVVDVRYDGELPSILSSLEVQGHSVRLVLEVAQHMGDNTVRCIAMDSTDGLVRGQKVLNTGAPIKVPVGRGTLGRIMNVIGEPVDEQGPIDAAEVWSIHRDAPEFTEQSTEQEILVTGIKVVDLLAPYQRGGKIGLFGGAGVGKTVLIMELINNVAKAHGGFSVFAGVGERTREGNDLYREMIESGVIKLGDKRGESKCTLVYGQMNEPPGARARVALTGLTVAEYFRDVEGQDVLLFVDNIFRFTQANSEVSALLGRIPSAVGYQPTLATDLGGLQERITTTTKGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRSIAELGIYPAVDPLDSTSRMLNPNIIGTEHYNVARGVQKVLQDYKNLQDIIAILGMDELSEEDKLTVARARKIQRFLSQPFQVAEVFTGTPGKYVDLKDTIAAFKGILEGKYDDLPEMAFYMVGGIHEVVEKSDKLAKEVAARKDESKKAKSAEALKDVPSLDKLVSEIKEEVVDADDGLEEDFKAEAISSENMVLNEKGEKVPLPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHH7</accession>
    <name>A0ABQ5SHH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013908</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF213">
        <property type="entry name" value="OS03G0770700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="16"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="132"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="279"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="586"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="798"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="849"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="962"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1103"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="591"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="963"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1109"/>
            <end position="1126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1126" mass="118039" checksum="28A1D3B70245A192" modified="2025-10-08" version="1">MEALALVAKASSRHSLFKGVTLYKPTSKWRAQISHGGRTVTLGDYSTEEEAARVFDRACICKYGKDAVCNFPVDDYKDEWEELLSTTIPALLVKFKEERQRNKINRTAAASKSASMPAGFSALPLATESALQPLPRPRQPAKITQATSRQLPPQPHQGPPSQPYPGLSAKGTTLGSAAPLGIEYCFEGDCSGATATVKHGTQGPMGHLNERDRKRADEFLSRGALPVAAKAAMAASHGIGHCQRDLRHQAFTCQHRLAMSSGEQKLEVDEPDSADFAMAASAPAPSSPTGVRRRATTRPARAEVSADGATGTSSLMALPRTSSSPNLDHLAPALWKAHSLPNTASALDWSTPEAAALTEEIMAAWDGAAVATLQEDGAIASAQRPLSLPTISRVHRNTRYPASWNRSEDHNPAEIWEGSIAAEPMRVAVASQQRVVHVVPAARRPPPVEEFRAGSKLGKGAIGDEPTFKVDSVARVSSDRLEARGSPLLAATRRRVGKRPDEEADAEVLEAEEVCGSLDAAAPAGPSAAPMANAGRGTDRGNASPIDIAFDPTYTATMARGAFRRTRPTEWQAGKRMRPAAAAAAEEEEAMAPMRAGEESLGTTTTTTSAATEEDCGRPSSQGYVEEAYGSARHWHGPLTPAAAAAWCAAAAAGAGVPAATAALPRGMFWGPVWPQRLVEQEPYAPAMGRGLPSARRPCSANAGPFITAPTAAATASAAAALAAAEDLSVEELHQVLLSALQRREQHLIKQQLQQQQSIRWQQQEALLRLVEAAGLRPVLSMDTVALLDELSATASEDKPMAWHGSSGGAAGGSQSRKRAAGDGNGASDKLYEVGVPDAQMRATRQLVAPPLQLPAPPQQQRKSSGGGDMAAPPPRGLLGSAVTPDSPTSSSSGGGGGTRDVRFLADVATPATALPAATTAVTTTAAPANKSHHLSPTVPLRTYADSDVNLGADCAGPPATNGDCSGSGSHSSADRSDVHNSALDSKAAQDAAPAAAITPNGGETAATGPLARPCQKRRLPDAPTYLVRERERAPTVRLSIGRDAPYDSYAGTSTAAIDDGHSIQVPLLLRVPSSVQQQILGMLQLQLADASQQSQPQVVRMQSLGTRQEGRSAAKARRIELVSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHN2</accession>
    <name>A0ABQ5SHN2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_014085</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="9"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="109"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="234"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="336"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="373" mass="40159" checksum="2D63DCAD9C906C4E" modified="2025-10-08" version="1">MAATQRTEKNQWNNMIAGGLAGSVAVLFLHPFDVIKTRLQVQDGASLAVPQYRNAIDAARSVLAQEGWRSFYKGLTPALIGSGVSWAAYFAIYEAVKAWHCRWQERDRLSPAWNMASAAQAGAMVCLLTNPIWLVKTRLQLQRLPLASAPAAAGVAAGGAAAHQILPYNGFLDAMIRIGREEGLRGYYKGLGPSMVLQTMHGAVQFAVYDELKYVASRFGRSSEEADRQLGSGELSLFAASSKLTASVTTYPSQVVRSRLQQRMDDGRTLVYNSASQVVRLTWQREGLRGFYKGLGPALLRVMPQSAVTLVAYEHILRLLDAASLQEAATVSAEAAAPTSSPIPVSPSALASGPDVRNVHATGRSQTAPAATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI89</accession>
    <name>A0ABQ5SI89_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045120178" evidence="1">
        <location>
            <begin position="22"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="199" mass="20945" checksum="BA1804FC8A0F7354" modified="2025-10-08" version="1" fragment="single">MATPQPCRSLVLLVDALHASAASTLRLALIRFSNLLALGTASTDRPTDVGLALILRSGTSVQLQVVYKPSRFMLRDFQAAVHRIRENEAWASPDTRKLVLALRSLVQHLAAAAGGGAGDPGLVPGVQVLQAAGIMRVVVVAHCFKPPAGGLLMKVLDEAAQNLVSVSFLSLNTEHSLELFRLGPATATAIPSAALPDMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ10</accession>
    <name>A0ABQ5SJ10_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014091</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033443">
        <property type="entry name" value="PROP1-like_PPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932">
        <property type="entry name" value="ATPASE EXPRESSION PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932:SF44">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17177">
        <property type="entry name" value="PPR_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="304"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="PROP1-like PPR" evidence="5">
        <location>
            <begin position="311"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="340"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="375"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="410"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="445"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="480"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="515"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="550"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="4">
        <location>
            <begin position="1025"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="187"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1130" mass="120554" checksum="76A09F830DF4CF93" modified="2025-10-08" version="1">MWQIGQVSQKQHNNQRTPIQQNHSFAQQDAAAAASSSASWVRPILPAGSSGSAAGSQQYAHSGGMLLPTSFAVLSPAQLSIASLHQQATQLATSIPQQQQTMHQHLSIQGRPVQQPWTVSQAWAVQQPQPLLAQHIQQQRPLQQQQQQLVQQQQQPAQIPLPHPAGGRGWGGQRLPYPRPGGQLTDNYAGVPVGRGRGRGVGGSDAGRGWRGGPGGAGRGGAVGGPTGGHSDAMVQQLFMVIRQLPAGQAPQDAVADRLAAMDGRSVAMLLKQLANNGLAMLAWELFDWLRSLAPSHELVRLLDVFLYTAMISLCSSNRCDMEVALALNREMVARGVPRNVHTFSALMNVCIKAGQHQAALDVWRELQEAGCRPNVVTYNTLIDAYGKTGQWAEALKVLARMKDEGMDPVTRTYNTLMIACNSSNQWHEALAVYNQMLANGHSPNTTTYNALITVYSKAGRLDKVMETFQEMEAAGCERTVITYSALISACERDGQWELALQMFNQMLREGCIPNIITYNSLITALAAGSQWERAADVFVQLQRQGCEPDVVTYTALINAYEKGGQWRRALQAFDRMLQQGCRTDHVVFSAVVDALWDSGVTVAQNRAALIHCAAMATGLVKPIITTAAVLATTPAAAAASAASGGAGPSAVPSRPPGAYSTAAATADLSAAGPAASESGGMSEVRELHLASMATGSAVVAFLAFLADQRERTIDLGPDAALPPRVILRFSRSRGSREGATTLPLRDVLHNWLQTRRSPFKLVQDGVAAVSQRLEAPGSDMAAWLLEDPVAVALLPYTRSSIAGALAGAVRDPLVVALEGFDDEVVQDAELEVSCRDAFEKVRRFEATHCLSVAAMGIAYLGRRRELATDLLRVAATLQLPRDVTHDAVLLLDRLMSAAPQLREDLFPMAAVTALRILAVSAATGQQVMLVPGDTNDTSGLRGNGGGGSSAKVEAELDARLAQVTGLPTLGFQSMELNIHSALVGDMDAISALRCLRLYMHRLGTEVEVQEAEDIQQDRVGPSLQVLIDQAVRDSEFLNYRPSATAAAALYCHRLRRGQTPFWPAAVASLTGYADLQHPELAAAVGAVQRLIMELSAASAIGVASSIRVVGDGSSSGAGTAQMPTLIGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ18</accession>
    <name>A0ABQ5SJ18_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="532" mass="56685" checksum="B1474F7E467DCDC9" modified="2025-10-08" version="1">MDPLDVDSSAVLADLAREYERSCLVITQIEDRLKERFMACSANGTVEVEHLVPENALDPPAEASENDASVTGNVGFSVLAKRAAVRMLGSFSRKLQLPKRASLGCSEAAEATRRISVVDMSAAALPKRSLPRRAASFYSSGSAALQSTTGLNRRAPVNSLRQQLQQELEQQQHVQTASDATSSPAATSKTVSSNYGPVTTIMLRLNSEHIARHRQNGSEPPKSPHQTVLPDIHALPLRSVHSQPLQSLQLRGSTRQQLELQDSIVGDGLMDIRGDTFNQLPQPLGDDGTRWRSMSRRGSDYIFSPLGTAATSTGPQQQQQQLRQQLQQLHLQTPGSPAPSAAASGCSSPATGASSGLPTIPAAYGGGFYFSRPIRRSSSNNPLGLRSSSRTQMQPSWATDELEVFPACSVDDSGGRSATVPLIITERAFPEEEEREGEIAPRGATVDLDSLRFLSPVPDTGLAAAASIPMGASYSRPAGGALPRVRVNSPVGRTRSYTNIMMGTSPTGAGSQLRTVLSTQRKVGPATSQLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJB6</accession>
    <name>A0ABQ5SJB6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014839</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029602">
        <property type="entry name" value="IFT74"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31432">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 74 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31432:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 74 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="152"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="644" mass="72208" checksum="CB017FC96F6F7E48" modified="2025-10-08" version="1">MDRPSSRGALALSHSGGLGKAPTGSAVPAPDRPMTGSRGPASGVPVGVRPPGGSIIGGVPPGTAMRGGPGPTGGPPGTAYKRLGTASQRPGTGQQAATAAAAARAGQSLQVENRPITNHGVSGMKTAAAGVGRQVLDKNYFMNELRQKRMEIAQVTQQMKSDLEALERKQAQYNSMDKRANDLSKEVKILQEALADYNTVLDKVGSQTPIFMIQQEHTALRDRNEQQRKRVDEVLTERLNLESKAKQAETKMAEIQASMDQRLNSMPPSQRQQYAELISEQQQLQADSKRFEEVLEDLDKQLQASEGELARNPFKQRSLQLQEQIRALTERKYELTEEERQSKRSPEELRADLMAKIKRDNTEVENMTQQIRDLQDQIKKMEERVKSMGGATSGAAAAEEKANREKFEELLAKERDLNNFMDGFPSRKAAKMQEKQSKEDGIVAILEKVVKMQGIIGSALPSQKKFKEMQDELEYKKMQLENTQTTQERLKEELSMRRTELEKIDTLEDKIKLELTQLADRQTAMEKEMTEFGSVEEIQRKAQLTRERMEGLRSVLLKRKDLLRSIVAEKGLKFQAKRAQLQDHSLQVQLEKMEVKLKTLNAGVFEMNEFIKAKESETNYRQLASNISSLVDELNVLVRQGVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJQ5</accession>
    <name>A0ABQ5SJQ5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="473" mass="50407" checksum="0D16123DCFD800A5" modified="2025-10-08" version="1">MLSRAIKAHVSMLCVRLHPPLPPFPAVSRLVYWSFPSPSASPSPMQTEPITAHIWVNFDHSAYLRCLQTAAAACRRLDLRQLHVSWAVSEALCRISGIPTGPLPAWMWPRVRENPKLKLLSDNNNNDYGDHDDDGDDGDGGDNGSCSSNEMGTLSAKEFLTELGAVVITAAEEAASVPGELQPVRYPRGTRVRRGSGGGEEHITEDAGMGGVRGQQQHMDADRQILKGLVVTVLPPPVDPTASPSGGGRRTPTRPEMASAQDGDETACDMMQPTGEGGEGGCAAGRAIRDGPPCGSPGARRREAAHGQHQVMGRIDVCRVGPQQSEHFAHDQVGETAFDKDEHPAVQAAVQMYCVPHSPESLEAAHERYRAVLRCLMATAAVTADAAIMHPSDAGRIQGSPGNVDTDPACTTSLKGGGQKELRPPWQALWRAGTLRSVDDPAATPADDHVEGSGSSPLRRTRFRAAKAPAGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJR6</accession>
    <name>A0ABQ5SJR6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46565">
        <property type="entry name" value="COLD SHOCK DOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46565:SF20">
        <property type="entry name" value="COLD SHOCK DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="243" mass="26560" checksum="42C99DA4B8373A75" modified="2026-01-28" version="1">MGEQLRQRGTVKWFNATKGFGFITPEGGGEDLFVHQTNINSDGFRSLREGEAVEFEVEAGPDGRSKAVSVSGPGGAAPEGAPRNFRGGGRGRGRARGARGAYAAYGYPQMPPMYPGYYFFPADPTGRGRGRGRGGMPMQGMIQGMPYPGMPMPGVGMEPTGEPSGLQVVVHNLPWSCQWQQLKDHFKEWRVERADVVYDAWGRSRGFGTVRFTTKEDAAQACEKMNNSQIDGRTISVRLDRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKK3</accession>
    <name>A0ABQ5SKK3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Piezo non-specific cation channel R-Ras-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014963</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13167">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13167:SF25">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="495"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="521"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="571"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="611"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="664"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="690"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="898"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="924"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1168" mass="120590" checksum="5899E7D0A09C7729" modified="2025-10-08" version="1" fragment="single">MKRYTGRALIAAAALATEAYNSLSPAGTIFLALFLVWGGLATRSSLAPPREGARANLVLWSFASAAACVALLLQLLLPLLPYGGNGPGSLLWAVLGLRDLWHCSAWELLTIFAPPLLCALMALAEAQDAYTACCWGATVSSSGGSHGVAMPDGRSSGAPAIATDASAPDHEVSTTGARPAFLSPQQAAEAEGSSSVAEPAPECSPAEGMGLMDHRTVSRVVHSSGSVVGTGGSGTGSAPAASPSGYSAAACMLILLAALLWPAVFNLPWLLLASLSMAMWALRLASSPSMDAPLLRLSQLYCGAALGALYVWQLRTPDAWPPSIAHGGRALGLYRFHDTAVEAHVYNLILQTLHAAALTSLYAALGFAAGSASESWRRRRSRHVSRGLATSPATAAAMAHVGRSPAKTALREPLLPPLQAILPACGASAAAFTRASPPAQAPIREGIAGAGSSGASSDRAAPLEARSLGEGQQQQHPLGSRYHHSHHQDNQNPSGGGHALVAHVAGWVVAAVEALTSHPSVAAVALAGLSMVEVSVLGGALLAVGMWALLAPGRYGRRMLLRVSPALTMLLLTWNTAVYVVTCLSASYPHLLPPVLHSLGLFMYVSPPPVVLPLTGQALAIVAMAGLARAACGANSPVSSISNRSSGGGGVCAGIGDNSARLTLLLGLYHILHVLVPSSWILLGSYKVDILHGVYLLAVMLYCGATAVRLIPHPRVGAILPDLRTIGDEGGILNVVDEDVEDGVTTSAAAAAITAGSSGNGVVRAAAPPPLPAAPPAHRLLRLYASMHLLALYAAMCGQLPGLGFLQPEAWADVLRLVGLWAPRRAANCLPLLGALVLATAHAVAGKVLTATAATGGVTRGSQLSAPARPLQQLQPQPPPITAPVAIQDWMLALGASLGSEASSVGNLLISVLLYVLVLYDMRIGLLGLGYLALGLPLLLMPPRRDQYVALLRLSDHRSGQQHASGQPQHLILSTWHSRALLSARIRWLAPAALALYCALDLGTSYATAMLAVYGPSSGQDTGAAGSSTGKIAEWWWRLVGLLYGGGAPAASGTPLVLTLARPSSLLLAMQLYRWAFAASAVRNWMRRNGCCPGASGNESEGERRLTALGESLVSSEGVSGGAATATLPQARRLAKQASAAWFVKRWIILNVDLFSGTLLFAAAMASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL15</accession>
    <name>A0ABQ5SL15_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-lactamase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014578</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012223">
        <property type="entry name" value="TEII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11487:SF0">
        <property type="entry name" value="S-ACYL FATTY ACID SYNTHASE THIOESTERASE, MEDIUM CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11487">
        <property type="entry name" value="THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="3">
        <location>
            <begin position="21"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Thioesterase" evidence="4">
        <location>
            <begin position="624"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="494"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="965"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="529"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="970"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="981"/>
            <end position="990"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1018" mass="106425" checksum="0FF760E4823013DE" modified="2025-10-08" version="1">MDFARPDAIEAFLHLHVYKELQEAGVRGATVCVSKLGRYSFTLPTCYGFADASRGVVLDPRSSLFDLGTCAKLLAALALLQLLQESGLTVTADVNSMLPPSLKIDTSRYGTVTVQHLLTHTSGVRRGNGGAGGNPDTRAAAAAEDDSSSAAAAMGQPQRPSSGRPPPEGPEGHTQVPERGADTLVMSYLRAQPVYCAKPPGTYRPCDVDYMLIGAIIEHLTAEPYDTYIKDVLLDPLGIRLPPQPGEPQGGEGGAAAAAAMTTVLEAADGTAGDRPSVFVHVRQQELDQARPQPRAQDGDPNQGGGPMGPSTSEGAVNAGGGAGMEVVPLREAELAMEKKVLARYDPPPLTPVGGLVLTAVDIGRLLPLFLHNSTAAGGDQDGAGSGGVGRSLLASLYEIRLYDFTKDVGVAYGGLVALGLGHCGDRVVSCESPPPPPSTREVALTAQAAAQAAAQAARAAAAEAAVAAAEAEADGSPVEAVAASGAEQPIADSQLPQDQDKDSQQLSQQQSQERVGERDQQPRPPAGLSGGGVSGGGVSGRGSAGGGAGTFNSWGHPSLLLVAPDSGFALLVACNTAGPEGTTFCRNVAAKVLERYRPAELPRSAVRSWLAPVPPPRQDAIVHLVAFPPSCCLNPITFFDGWSLSMPDYVKLHCMQAPGHGTRRHESCELRLTKLVPPAAAALLAGLPADAPLALFGHGLGALWAFEVARRLECLYQRQLLHLFVCACPAPACFPPPMRAAGSAGNSGAGGEDGYVRRFQSWWLPEQQRQVLIQQKQQQLMHQLQRPGSGDAADGVAGGDVDIPELYELHDEMFITACRNHPRFPRQLRNDIKAILDHLPLLRADIEAESRYTFLPPAPSLCPSVAAVLASVQNRADDEMRISQGLLSCPITVMIPENETALGPGQTNGWEQVTTGLVRFQAVAGDYHCLEDRKTRGQVVAAITSALGRQMSVHTPWLAKLRPERAGGRWAGTGGGRPSGGSSRPSSAVGGYGGSGSGASSSGPVLTLHLPPMYPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL17</accession>
    <name>A0ABQ5SL17_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Farnesyl diphosphate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015530</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039702">
        <property type="entry name" value="FPS1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525:SF0">
        <property type="entry name" value="FARNESYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525">
        <property type="entry name" value="FARNESYL-PYROPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="40928" checksum="B9FD5BD1FBD5709B" modified="2025-10-08" version="1">MAEPTPKKARVGYEHTREDFLKVYEVLRDELLNDECLSSANESAQAKAAQEWFKEVNDYNVPGGKLNRGMAVYDVLASIKGAEELTEEEVFKANALGWCIEWLQAFFLVADDIMDGSITRRGQPCWYKVPKVGMIACNDYILLDCCIYRTLRKHFRGHSAYAYLMELFHEVTFQTSHGQLLDLTTAPIGSVDLSKYTSDNYMRIVTYKTAYYSFYLPVACGMVLAGIENPAAFDLAQKICVEMGQYFQIQDDYLDCYGDPQVIGKIGTDIEDNKCSWLVCTALQVATEEQKEVIKVHYGRKEPESVAAIKALYVELGLEQRFRDYEATSYATLDATITEQTLLPKEVFSSLLAKIYKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL60</accession>
    <name>A0ABQ5SL60_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="100" mass="11176" checksum="719769D6EDEBA4B3" modified="2025-10-08" version="1" fragment="single">AIPPDRFNDLVTELEALTSTPSPISPPLELLSTHDTSEYYFWADQGRQLIDSIPSSFDKVEMAVLTISEVRRHRATFTSQESTAFTCFLLVTSNKLGVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLG9</accession>
    <name>A0ABQ5SLG9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015205</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045793279" description="Peptidase M20 dimerisation domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="7">
        <location>
            <begin position="130"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="416" mass="43510" checksum="67FA1763CD94C948" modified="2025-10-08" version="1">MPLLPVLFLSHLDVVPVANETLSNWTYGPFSGAVKEGYVWGRGALDDKVGVSALLEACTLLLREGWRPQRTLYFAFGQDEEVGGEMGAALIAELLARRGVMVDIVYDEGGSVGADGFPPYTTRPIALIGTAEKGYATINARVSAAGGHSSLPPLDGSSAAAVAARLVTSIDRRPPQASLVEPVTSLLAALAPSVPGLRGWLFALAASGLGSPWVERLVAEAFLAHDAPETAALVRNTAAITGLNLGFAENVLPPAATVRINFRMLPGSNISALMDYLHRAAGRDLLHLTFEMDGGQNASLATPVTSATNPYFQMLKTALQEVYQLADGGGRVDVAPTLMAGGTDSKHYLPISRGGALRQTPVSLNRTAGDGRRVHGLNERVSVGDFGRAVCVMRRLLMLVGTLPAPAPASAPAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM25</accession>
    <name>A0ABQ5SM25_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="99" mass="11101" checksum="D9688E572DE92B8D" modified="2025-10-08" version="1">MVLLLVEGRKLQQDNLLCQWDSMYECYRRMGRLVERQTGRPAAMARGGKGPAAVAYRSDNDSAVWQVEGPATHSHNIESLLGSYSPSGPMIHGYKRSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM45</accession>
    <name>A0ABQ5SM45_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_015472</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF963">
        <property type="entry name" value="AT08303P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PB1" evidence="8">
        <location>
            <begin position="683"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1005"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="34"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="317"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="783"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="783"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="806"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="830"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="860"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="1032"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1338" mass="135383" checksum="8EF2A574D4249795" modified="2025-10-08" version="1">MACFGMDYRFHSDFAAWPVPVPYSSQWQSNLRVGTPAAGTGAPPSPPAPLWKPLHPEPETLSPVIPRHQQQYHQQQQQQQELARNQMQQLQELQRQLGEHQQLLSAVIAQAAEAAGTVPAPAGPTDAVLALPPGDAALATLPDATFVPGAYTALQLAQVSGAGQGIGPGLASLAPAAPPPARVTAAAATGTRGRGSLAVPAAAAFPAAQSGSPFSAPTTVDATTAAQQLALSSPAALAALAASGGLAAPTSITAAADALAAMSIAVRQHQQWQQQQREQQEQYAAAAKAAHAVLYPPQTHLQHSLLEQHQQGMVLPQFPSSRSTVSNASEGPPSSGGSGSHPYTSMGSLQNSPCGAGSTPSAYTQPRAASAAAAAATAASFGEGVGGVVEASDTSSNSAAHSEESAVSFTRADLVIPASGSQPAQTVAVTVATTSNGRQSVTLPAAALSQIIPMSKAAAGAVLLPSPQLTGLAAASEATPAAVGAEHNSPLTSSPAATATTLANAAAVLPYPATVTNLVGTTAAENPAALLSTASAVTGSSAGGINPCLRGGGVSGTTPLTLQSLQGTLASASLANSLLRPSAPPYGLQAGVGVGLMAGESNGGNSVSAWSSPSLSSGMGLGSGLGPPGSEAPVALAVMADTATADTATAAIAAATAAARQGVAKDVKVKVLLNSGGAFSRCPSGVWEYNGGETRLVAVRRTETYRELVSAIARAVSMNTNDEALLILKYELPGLPEQLVDLLTDDDVANMWEALEEYCIADQKPTFKLKILVQQAGQLCSAAGSRTSRSGGAGAGAESSGRAAGDAGGGGSGFSTSGSGGGGGGGGEGKASSGSSCSAGAQQQRSLGPPAPTAAIGSAAGRGSGGGGGGGGGASVPDVAAVALHPLQRKSGGGAPHLPSIPEFDAMIAGRVAGAPTAPPNLAPPEPGTVQAAATRAAAATVVAPASGDGGGGGSIMPYDTVAASEREAEQALLRSEIGSLVTTDPRVSLSDLESKLEIIRPEDLQIIKFLGSGGYGEVYLCRWHSCDVAAKCLNPSLLMPDTVGGSSTMGSDVVTEMLREAAMLGGLRHPNIVWVYGIVLPSGVREVKAKRERLDAAGDIDVVRMTAALAAGMPIVPGVVRPPALVTEYLGAGSLRAALTRGSDFLRSDMVRVKLALDAARGMEYLHLKRIVHFDLKTANLLVGFREKSPTCKVADFGLSKQRQQTYVTGVSSLRGTLPWTAPEIIRTPRAVKDKADVYSFGVVLWELWTFREPFEGINYHALLHQISTAKDPVRPSMPGCADWDGPEPEEPAPGWVGLMCRCWDENPDARPPFTQVVSELESMLAALKSKRRATSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMC3</accession>
    <name>A0ABQ5SMC3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CASP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015982</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012955">
        <property type="entry name" value="CASP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043">
        <property type="entry name" value="CCAAT DISPLACEMENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08172">
        <property type="entry name" value="CASP_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="813"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="CASP C-terminal" evidence="4">
        <location>
            <begin position="469"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="CASP C-terminal" evidence="4">
        <location>
            <begin position="762"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="132"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="447"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="593"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="878" mass="93292" checksum="6B7EDEB10D0BC233" modified="2025-10-08" version="1">MNFLKTLDQALDFERLEKTVTERVQQAALSVVEEFPDRHRAAPTGALLTITPNVLVEAREVSEIWQAYEMEGRKDEWEAACEELKVLGRKDETAHIALQAWQRRAHDAEHKLYQLYSSLLALPDPAPFLFLLSSLAARVQDLEDLNRKLQVAADQAAKDDAEVTSLIDKHQRLLESTKELQEELARRDARLSEMSAAAAEAAIARQEMSLQAAEMARLQRSLRSVKEEYEATQQSLFELQSRLENLAGSRQQEAELASEELERVTKRAAELELERRQLLSRLETAQQAAAATGGGGLMDAGSVGAASAAGLGGGGGSGTGVAGGLLGGDYVLLERQLAVREEQVSALSSSLEAVQRGAADAASRHALELATLKDQLATTRMALERLEREVARRPEPKAYQELRRRVEALHSLVDVQMQAEGWPGEQVEATLRDPSTQSVTTILQERSRKLSSEVNSLKRQLAERDKQIQDLEGQMKELESDLQRRAQLVAQLEEHLAAAAGAAASAAGGGGAAAATGTAATTPLVGGGGSEPRMDSVLLPQLSGSLGIIHSGDLEASPPTSSLVAAGSSDAVAAAASHGIGGGTGGAGGGSLLEIVVSQRDRFRQRMVQLEEEKSQLSDELHKTQQQLQSCRNDNVTLFEKIRYLERFNQRAAAASRTTVVRVDAAGVAQPSVEELAAARTNRYQCGPFALEVGGPRRGGLDVAGTAGGGGSGPGASGVTKYGGSGSGVGVRSRGARRGGAGALACLGGDGDDVEASGTTPESRAARAYEARVNPFAEFQQSEAELRVRNLQVHDRALLAGSRLLLGSRVARMFLAIYTVMLHFIIMLLFFYATTPCPAQAPQLDATTLVSAMSAAASVTTGAGNSAATGTLTSMASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNL0</accession>
    <name>A0ABQ5SNL0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial inner membrane protease ATP23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016845</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="23573" checksum="2BC81ACCC5FAC811" modified="2025-10-08" version="1">MGKISKVSGGGIGGYSKQETADRSGTKKVGSGIAAAASRGGNVTASGGGGGSYSLEIREGCVALMRFRRRDSMNVLLDPISNAMEGVIANRLGHNFALSDTAKDHVAFRKTCGLHKQVKYVIATLDGDAHSIHHEMCHARYYLDQSYKSTVMQVWSGALTSAQRDAVMSFLNRLKYDAVAHVDEFQAYLVTEKSNFFGMDLADAQQQLQASFPPGSWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPE3</accession>
    <name>A0ABQ5SPE3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="18980" checksum="E5BDA53B7B9B0B4B" modified="2025-10-08" version="1" fragment="single">MSAEHDDTPHTSLASQPDFVVAARAPDRGLETVGTRATQDPLFLQTPAHLIVPHSAVNTNMDNTAGPRVLPLPQARGNDDIPEVMRLSIPVPRLNISLTDKTPGHSIADATHAYVRDAKKYLQAVHGGDNDGIRCLFVGNTLEGSAKDWYRNWTNARGNYTFDELVAALLARFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPH0</accession>
    <name>A0ABQ5SPH0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="148" mass="16420" checksum="698C9CC16E60A790" modified="2025-10-08" version="1" fragment="single">RLRHGWKAYIDSPAEKAFVTKRLSDIASGPSSTRTTAGGGASSSRQPAPREFQLFWQALLGLPPIVAGSFLTLPSNVYLLGSMCWGSSLLVRHCYRGIFDRMMELHSSNMKRQFLITGVPMNSSRLGCFTSYTHRSHSYLFRDVVGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SR57</accession>
    <name>A0ABQ5SR57_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_017193</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000125">
        <property type="protein sequence ID" value="GLI71827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF62">
        <property type="entry name" value="SERINE_ARGININE (SR)-TYPE SHUTTLING MRNA BINDING PROTEIN NPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="10"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="108"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="188"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="200"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI71827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="286" mass="33874" checksum="37C19B31A783D218" modified="2025-10-08" version="1">MARWDRYGGRAIFVGNLPIDVREKELDEIFYKFGRIRMIDIKKPARPPGFAFIEFDDPRSAEEAARRRNNYEFAGMRMRVEIARGGESGGAQQPLRIGYRPIRNTMGFRLYVKGLPRSASWQDLKDFVRRVCKPLYTEIFKDHRDNVLGVVEFESKEDMKAALRKLDDSEFNNPFDKGHYVRLVEDLEERGRSRSRSRSPRRDRERERPRDRSRSRSRSRSRSPKRESPSRSRSRSPAAKSRSRSRTRSRSRSRSHSRSRSPEADNGMEVDRDRKEERDEDERRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMM7</accession>
    <name>A0ABQ5RMM7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Plastid lipid-associated protein/fibrillin conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000594</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039633">
        <property type="entry name" value="PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006843">
        <property type="entry name" value="PAP/fibrillin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31906">
        <property type="entry name" value="PLASTID-LIPID-ASSOCIATED PROTEIN 4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04755">
        <property type="entry name" value="PAP_fibrillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0934">Plastid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Plastid lipid-associated protein/fibrillin conserved" evidence="5">
        <location>
            <begin position="89"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="352"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI58830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="390" mass="41526" checksum="0CFDA01BD16754FD" modified="2025-10-08" version="1">MLSLRPRNAMATAASHKPSLLVHAKLCARLPSVVRRPRNNTLVCRTGGIINESVASNAFKNSESNTAISLGGPEPAAHHLDEARHIVDEVLEAIKETDSGLQISPEARARVDSLLYRLEALGTTQQPRPLDNPLLWGNYNVAYTSVGRSQERGEPAGGRFRGRIGRALFRTAGLFQSVLKPDIATNKVEFRLFGFLPGYVGLRGRVQPQGEHGDTVQVIFEPPVLSIANSLHLKIGKPSSVVLSTTYLDERVRLGKGSRGSLFVFTRGGAADAAGMGQVGLQRTSLTAAAAFTAFFVSLFLGGAALWVSGQLVLKAAALAMWLLGAGMGAVVRNGGLVMDREDERMVAAAKAELERRRQEQEQGRGTIRESERVDGTGGGGSASATPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMR5</accession>
    <name>A0ABQ5RMR5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="167" mass="18219" checksum="1FF33333C2EDAF66" modified="2025-10-08" version="1">MPSYWGVSTQQNQLLWILFACFLITGGIMLPLGWAKLVPCVNTYNKCIRNYYEFTCRSDRDECAAQWARIWVVGAIMLGLAAVPLCCFSCCSGPPTKVTNTQPPTAVATQMGAYPQHPAAPSQPYPGRTEIGYPVTVTGLPQNGQGLTYHYPAPTETVQGSGRYPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNB8</accession>
    <name>A0ABQ5RNB8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CHRD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000324</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010895">
        <property type="entry name" value="CHRD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07452">
        <property type="entry name" value="CHRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045949917" description="CHRD domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="CHRD" evidence="2">
        <location>
            <begin position="61"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="189" mass="20302" checksum="375B0EE819E76A19" modified="2025-10-08" version="1">MDKHVPAPVLWLTVFVLFLAVGNGAPVWPDGPYGSLVAVAKLIPVDKSLVVRGSVALVWPRPNMGNASVALNLTGALNQITMAHIRLNATGAPIRLDLLPRYPNTAPVPLNPPLNFRGTLGFTVPFDYLRVGAWDPDNDHMNFIQLLQKGLLYVSVQTVSLPRGALRGRLECKPPCMFSIGGGAAHPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNU4</accession>
    <name>A0ABQ5RNU4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06168">
        <property type="entry name" value="LSMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034110">
        <property type="entry name" value="LSMD1_Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF5">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 38, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="56"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="166" mass="18951" checksum="B90647B8EC1B2471" modified="2025-10-08" version="1" fragment="single">RQQNVHHHIQVNNILQILIPILINKADLLVVRVVVMGNKSLPSIGNEPRPQSVQDVRKLLYRKFRVTLISGRVLEGDFTCLDRQGNIILSNTFEQVTTASGRREERHMGLVLIPIAQQRKIELQATIDEEMSMLKLVEHYAASPREDQVVLVMERDTALAHRDSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNV5</accession>
    <name>A0ABQ5RNV5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mg-protoporphyrin IX chelatase</fullName>
            <ecNumber evidence="10">6.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_000805</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>protoporphyrin IX + Mg(2+) + ATP + H2O = Mg-protoporphyrin IX + ADP + phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60492"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.6.1.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Redox regulation; active in reducing conditions, inactive in oxidizing conditions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Porphyrin-containing compound metabolism; chlorophyll biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The magnesium chelatase complex is a heterotrimer consisting of subunits CHLI, CHLD and CHLH.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">The magnesium chelatase complex is a heterotrimer consisting of subunits CHLI, CHLD, AND CHLH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Plastid</location>
            <location evidence="10">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.6.1.1" evidence="10"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.80">
        <property type="entry name" value="Magnesium chelatase subunit I, C-Terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011775">
        <property type="entry name" value="Mg_chelatase_ATPase-isu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02030">
        <property type="entry name" value="BchI-ChlI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039:SF9">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLI-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="10" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="10" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Magnesium chelatase ChlI-like catalytic" evidence="12">
        <location>
            <begin position="159"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="ChlI/MoxR AAA lid" evidence="13">
        <location>
            <begin position="309"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="386"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI58984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="433" mass="47044" checksum="9A7DB4C8386C5BC7" modified="2025-10-08" version="1">MQTINGQRAITTARRCRAFRQRSGPALVVRATAAAAPSAKNSKKPNFPFVKIQGQEEMKLALLLNVVDPNIGGVLIMGDRGTAKSVAVRALVDLLPLIDVVEGDPFSSSPTDPKLMGPDVLDRWRRGEKLPMTQMRTPLVELPLGATEDRICGTIDIEKALTQGVKAYEPGLLAKANRGILYVDEVNLLDDGLVDVVLDSSASGLNTVEREGVSIVHPAKFIMIGSGNPAEGELRPQLLDRFGMSVNVATLQDTKQRTQMVLDRIAYETDPDAFAASCRPEQDKLTDRLQAARERLKQVKISNELQIIISDICSRLDVDGLRGDIVINRAAKALVAYEGRNEVKLDDIERVISSCLNHRLRKDPLDPIDNGTKVKVLFKRLTDPEVQRREAEAQKAKEEAAKKAKEGGAAAGAGRPAGAKAGSWSGLGVPTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPE8</accession>
    <name>A0ABQ5RPE8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D-serine dehydratase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001308</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DSD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06819">
        <property type="entry name" value="PLPDE_III_LS_D-TA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.20">
        <property type="entry name" value="D-serine dehydratase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051466">
        <property type="entry name" value="D-amino_acid_metab_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026956">
        <property type="entry name" value="D-ser_dehydrat-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042208">
        <property type="entry name" value="D-ser_dehydrat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004:SF2">
        <property type="entry name" value="D-SERINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004">
        <property type="entry name" value="ZGC:162816-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14031">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01119">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-serine dehydratase-like" evidence="3">
        <location>
            <begin position="299"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="430" mass="45758" checksum="E157B55E3F47CE14" modified="2025-10-08" version="1">MRLISLAQQTFVRRFPSRYGAIRCSATISADRASARIGDRLSGVDTPALLMDLDAFERNCGKLRRTMAPFPGVAVRPHAKAHKCADVARLQLQLLGAKGVCCQKVVEAEAMAEGGITDVLLSNEVIAPKKIDRLAGLAAAGARVGVCFESVNNLRQLQSAAAASGTCLDVLVELNVGQDRCGVDSPAEVVATARAAASMDNVRFAGIQAYHGGLQHVRDPQDRAYRVGKVVERTRAAVQALVAAGIPCETVTGGGTGTYRLEAASGIFTEVQPGSFAFNDADYSRNVQAHGSTGEWEQSLWVLTQVMSVSPSRGLAVVDAGTKAVSLDSGPPRLPPHYTQLYGATLEYASGGDEHGKLLWPQGAYQLPMHLPPLGTLLKLQPGHCDPTVNLYDWIIAVRSEQQFSGADVSREDSQVVVAVWPIRGRGPGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPN4</accession>
    <name>A0ABQ5RPN4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCDC81 HU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026295">
        <property type="entry name" value="CCD81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040673">
        <property type="entry name" value="CCDC81_HU_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14362">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14362:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18289">
        <property type="entry name" value="HU-CCDC81_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC81 HU" evidence="2">
        <location>
            <begin position="121"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="278" mass="29749" checksum="CA998E2CF0B66412" modified="2025-10-08" version="1" fragment="single">MFTEVDLFHDCAVKHNDIAPNQALGEHTYAAVWSALRGWLQALFARCRGAVFPTFARIAWYRAGGQMEDGAPMLRPIFVPSDAYLRAHLLHTGSGVMRASTMATEELNLEPCEEYSAMKIAIKHSCSLNKDVVFCCTRHLLHQLGEALHSGRPVALDLGVGVLSGRDRCLSFRFHSQYLAHWPATASSDRSQRQSPGVPLQPGLQSGSVSTGGSSTGTCGNDRIGVESDGGRGDAWCGSGCAGTTGSTSPGRGEYFHSNPGSGIQQQGQGVGDRGQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ10</accession>
    <name>A0ABQ5RQ10_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="302" mass="32840" checksum="15778E412F8F3321" modified="2025-10-08" version="1" fragment="single">MFGVVTSPPAGDNAGDCNGGAATGVSDPVVGIVTEFVGNGTLGQVVRSPNGRLSTRQCARIALQVALGMAYLHSQSPAIVHFDLKPENVLIQGEGENMVAKIADFGLSKPKHGKYVSCPTPRGTLPYMSPEVVFGNGEVSEKVDVYSMGVTLWQLHMGEEPFMRLSNHEILMGLESGTLHLPIPSTCDPEWRSLVETCMDPNPDNRPSFREIILQLQAILVQEQWTAKKSCSCAEVAVAAALAVLDSDKTDRTLPRLSPSPQLQQQQQEGLLLHFSSPELHQQQQGLLHLHLHRLHRLTTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQR6</accession>
    <name>A0ABQ5RQR6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-phytyl-1,4-beta-naphthoquinone methyltransferase, chloroplastic</fullName>
            <ecNumber evidence="4">2.1.1.329</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Demethylphylloquinone methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Menaquinone biosynthesis methyltransferase ubiE-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MENG</name>
        <name evidence="5" type="ORF">VaNZ11_001753</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the biosynthesis of phylloquinone (vitamin K1). Methyltransferase required for the conversion of 2-phytyl-1,4-beta-naphthoquinol to phylloquinol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>demethylphylloquinol + S-adenosyl-L-methionine = phylloquinol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40551"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28433"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:87844"/>
            <dbReference type="EC" id="2.1.1.329"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Plastid</location>
            <location evidence="4">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.329" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01982">
        <property type="entry name" value="MenG_phylloquinone_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032904">
        <property type="entry name" value="MenG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001244">
        <property type="entry name" value="PRK00216.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="300" mass="32430" checksum="D3BEAAF092380E06" modified="2025-10-08" version="1">MKTSIRSKYDSTGFHSWYARNFRPTPNSIPLAGGRMCTRIICTASGGDSLPGPRGAFSPGQEAAARQGLFNRIAPVYDELNNTLSFGQHWVWKQMTVKWSGARPGGKALDVCCGSGDLALLLARLVGPRGEVTGLDFAAEMLEDAAARQEAQRLEGMAPKAASITWVQGDAMSLPFEAGSFDAATMGYGLRNVADIPAALSELHRVLRPGCSAAILDFNNCTDPIPDAVQAFFLEQLVVPAARRYGLAAEYEYLRPSIKRFPTGSELERLAREAGFAVARHYPISFDLMGVLVATKGRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQX8</accession>
    <name>A0ABQ5RQX8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002029</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="308" mass="33530" checksum="D1B6202008916ADC" modified="2025-10-08" version="1">MPLHVIQLTGTGGSIPSTSYNPTVLLSAHRKERVTSGDPLYHGFARPSLCPINRRPRLPALRASDQNQTSIDDKGTSGPAVGSPEQQPSGDASFREPSNSTEPELASMWETIKKDVRKELNPEEAQVLDSIKVDDLLGDTGDLMKKLADEQLGPVLQSLGVSEEPLAFFVDLLRLGTTLQLLSAALLFYGMELGVGLDSGEALRCVCGLTAGYLSRPFFRVEQLLWPLYDWGLRILAPGAVYEVTVSREESQSALTRLGLAVAVCSFVPQLLLGWDTATTVQFVLPLAAGWLLFDITYMAALLFKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRE3</accession>
    <name>A0ABQ5RRE3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="670" mass="70414" checksum="E571039679D97AA8" modified="2025-10-08" version="1">MLSAGRVQQQARHIWVALGCSRHIDAALSSPQEHSSSSFRATSVASGDSDLVSSVGDADLRRLPVASFSGRGESSNDEIGRRSSGKIHCVEQRTRTFPSASIRRSTSSIGLMVAPRWFASGVVTAFHHHQPGWFTPTMWTSGLISASPSRRMYHSWYYGTKLRNRVITSASSLDQLANLLRHEGDKLDHINLVVMLRQLQLLASDAARKTGPTWASAPEGSGKVAAAVAVASSRAVEQQVEALVARTVRLVRRRTKWYDPRNAAAVVHCTAALGYTDGRVLQNMTARTIARLNEAYSRDLLLLLQGLGRHARLSASPSLENSGVPAPSGEPAAKTTARAGTVKALRKPKYRGPPPQLLKGVWQFLLAKIPTGRMPPENIDGLMRYVAALPRNQRPLDRDLLTTVETDLRVRLSIYAPKPLAGVLHVLASERHPVAPDLLDAAADQFVVHADTYGTGGAAARFLTAASALIQDLSRRGGGSGGGGDASKAAVPGGGEGTGRAAAAGPTEAAGLASETASSSAAGGGDGKGLSPGRWLAGKPELLALCLKLMTRDLSDPAPNRLVDWMAVLIRSEVPLASLASAATQTTALGRSALATPPTAGAIASSPAPTANAVALPVGYNSTAVATAAAAFVKAHSITVEKVATDLSPKAIAKVRKMYDTYGLTYPKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRH4</accession>
    <name>A0ABQ5RRH4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046141872" evidence="2">
        <location>
            <begin position="45"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="366"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="465"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="511"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="552" mass="54878" checksum="30CF9EBD739413AB" modified="2025-10-08" version="1">MLALGDHMFHKSPGMARARWQTAILWELQLFCLVASGLPHIVSGGQVALRGLQEAAANEAPQPPGSPTYPPYPPYTPNGPPTPSPTPTVPPSPPRFRRPQSPRQPISPSPPAAATTPPLLSPRTPPSPPPTLSLRPPPSPSPSPPPNSTLHSPALPLLAPSVFNLSIPPTGVPAAVLPPSPSPSLPSPSPPPPPPSPQPAPPPRPPLPPSPLPTAPFPPFPPPRPPIYAQSTKSTDGALIGVMVPLGLAVLAAAGFVSWKLVQVLQNGGTGRSGAVRVDLESGGRVPGRRGGGATSAGGASTSRSANGRAAAAAAEDTNPLFGFAKKARSSGPAANGNAGAAAGGGRAGRQPQRPPVGRRTHSPDESGSGSGDTTDSDSGSDQQDSGATQDTVRRGGGRGGTTPPPAARRPGGAAAPLMTAFAGAAGRGRGRGPPGPPARRPAAVQESDDGNSESESDGTDNEVSAPPARSRGGAGPSAGRSAPMAPGRPGVGPMAGRGRGVGNPAGRGGARVAAPPSSESSDSGESGSEDDGPPPPRRAPARPAPGRTGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRP8</accession>
    <name>A0ABQ5RRP8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="233" mass="22807" checksum="14C94F53E1B99F8D" modified="2025-10-08" version="1" fragment="single">AEEARPCGVAFPAGCDFSPLAPASYFTPPPALRPHSRPSSPQALKPSSPQALKPSSPQALRPSGPPASPPALQPSSPPASPPALQPSSPPASPPALQPSSPPALRPSGPPASPPALQPSSPPALQPSSPSALQPSGPLAPQPALQLSSPPALQPALQPSSPAAIQPALQPFSPPALQPPSPPASPPAPQPALQPSSQPSSPPALQPSGPAASPPALQPSSPPALQPSGPPAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTP6</accession>
    <name>A0ABQ5RTP6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="104"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="283" mass="32007" checksum="D29DEE2893306536" modified="2025-10-08" version="1" fragment="single">ENDSSVTQSSQADAQKPSPVDVSSVITQVTSVNSHRPTPSAFRVATSPPAKTWRKRFRSLLCCFAPQSQGYYRPQEGDFGVHRFVPPQPPRVHREVLIGPKRSDDLDKKTLVLDLDETLVHSSFKPIPNPDYILPVEVDGKLVDVYVLKRPWCDHFMENVCARFEVVIFTASLAKYADPLLDLLDKQRLVRWRLFRESCFPYEGNYVKDLSCLGRDLSQTIIVDNSPHSYVFQPANAVPISTFIDNMDDQELLELLPVLKELEDAPDVRIVLGANLGLRELSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUZ2</accession>
    <name>A0ABQ5RUZ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003841</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="88"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="387" mass="42993" checksum="506F8241F4CD743A" modified="2025-10-08" version="1">MGSRSSTRLALLAARACRNAGSKCTPLAMGEINAHDGVGARASTRNLCTLLRRSPTVSSQASCSGRLSIWDSCPLRLIHSTPATAATDYYELLGLGKGASDQEIKKSYYQLAKKYHPDTNKDDPAAAARFQEIQKAYEVLRDPEKRRLYDQLGREGMDRMESGGQSGGPEPGFEQGFQGGFQFGGMPFMNEIFEQFFRADPRLHAMLNRVQMPPIRISFMEAVKGTRKVLDIGLRPGSTRQIEFDIPPGIDTGDMVETAVDMPGPGRRASARMVLTIPIEVQPHPTFRRDGCDIATALQIPLTDALLGTTVRVETLDGNVELIVPPLTQQGDRLRIRNKGIFSPRRGVRGDHYVDISIIMPRILSIRQRELLLQFQNEEERKRTSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV73</accession>
    <name>A0ABQ5RV73_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003689</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="270"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="306"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="342" mass="37656" checksum="BFFEFA5C706F4554" modified="2025-10-08" version="1">MQLRPAQSSIGITHHTKCSTVPVLRTLPYLRGAHLLRCHASNDKDPKTNAPDEKQPNEAIPEVSGKLAVLLEELQKKGMDSKKAKVMLKKWRDMGVSDPEQLRKLLVRTSLRPAVTVGVQALLDGLACWGGFYTAGLIADSPPFAGQFALQLAASFFGFYYVLQALLNLSTVSGLLLTAYKYGTNSVELLAAVQQLAGPPTGLTVLDKAQVAVNTLKVLQTLDEIAELLKNMPTTAPQRSTLQNLSAYLTLAHAKERLGFDPARYGLSEAEAGEIAYVFSTYDVNDDYRLELSEVKRLCQDLGKDLADDELKEAVRILDTSKNGFVELDEFCAWWTKTQKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVH0</accession>
    <name>A0ABQ5RVH0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="113" mass="12484" checksum="3FFC2B2398792AC7" modified="2025-10-08" version="1" fragment="single">EGFPDLVAWWKFDEPNMDPGIFGHHTVARDASGHGNDLQLETPPQPRSAIIPLPSSRESLRTGALEFRNGLALNKHMQGFPGRSFTVEMWAKGAAVQDLNNLQQLSSQLLSYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVK6</accession>
    <name>A0ABQ5RVK6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23330">
        <property type="entry name" value="P300 TRANSCRIPTIONAL COFACTOR JMY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23330:SF9">
        <property type="entry name" value="PROLINE-RICH PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="502"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="721" mass="75900" checksum="26685C993A53BC28" modified="2025-10-08" version="1">MLVLIQHQIRAVRSGPFPQKLSSPAARPGPVRRCASPMDPPPPPPPPPPLPRSPPPAGGDGGGGGGRLPPSGSTWLRRIFQVTAGMLGAAGVLAAAMPSVLSTQWGLQYAVEALNAKLQPPGRVEVAGAQLSWLGGNELQCIKVYDSPRGGQILLHLNRVSTSSSLWHLLTGSGSYNVVIAKPWINAVYDARLSEFKLVNFLEKVGLLKPPERLVPTEQPKSQTDSQNQQQQQQQQQQERMLQEDCGKQGAAGAQGQAAHERQGQQQQKQQRHSGKLIEVLCRVNSKMEMNADVRVGRLSLVVADGMLLVPEEVRQILGPRLYVSGAIGDLELRQWAEEVGEDVTWAAPSAASSSQGGDVIPAGKLLLPPPPPAEVVQQPRHYRPGVLQVLSDHLRGEMRLWDAADHSLLHEPATASLDLTPALSRLALAACNPVLGNLVEVRGGGRLRGTFNPDGGRLPYQAATVEVEPVSLELGASAMAPRLLRDLGLSDRALTSVGSPATDVATNTNTNTNTNNTTATKSSTAADGAGVGDGDRAGPLRAVEMSSMRVHFHRNGQVRTDRVDMRLGGGGGGNRGGGGVHLEVWGNADLQHDELDFTLGVSPAPLLAALGLSAEGLPSGYLLLVPVRGAVSEPRYDVAAAAVRLAQLAARQRAVQWAKQREAGDGGGQGRLGALVSGLEALAGAVRGPQEMLAAIDRVMEADMAGVPPPPPPPPPPTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW15</accession>
    <name>A0ABQ5RW15_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDP-D-glucose phosphorylase 1</fullName>
            <ecNumber evidence="5">2.7.7.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_004281</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specific and highly efficient GDP-D-glucose phosphorylase regulating the levels of GDP-D-glucose in cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GDP-alpha-D-glucose + phosphate = alpha-D-glucose 1-phosphate + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30387"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:62230"/>
            <dbReference type="EC" id="2.7.7.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GDPGP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.78" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026506">
        <property type="entry name" value="GDPGP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058865">
        <property type="entry name" value="GDPGP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058866">
        <property type="entry name" value="GDPGP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20884">
        <property type="entry name" value="GDP-D-GLUCOSE PHOSPHORYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20884:SF8">
        <property type="entry name" value="GDP-D-GLUCOSE PHOSPHORYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26216">
        <property type="entry name" value="GDPGP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26217">
        <property type="entry name" value="GDPGP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GDPGP1-like N-terminal" evidence="15">
        <location>
            <begin position="197"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="GDPGP1-like C-terminal" evidence="14">
        <location>
            <begin position="425"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF26216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF26217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI61782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="573" mass="61094" checksum="D16C654DBE618C3A" modified="2025-10-08" version="1">MAATCELCHPSGALRPTASKPEATIASCLDLCVPVYTFSTRKFGDDIPKLARVDSFPVVPDCFGGCDPPMPHGDTAPHSGPLPEDSFNSLPVHMSQRIRQGAPSDVCALETVLCIAHDANAVLGSAETCTTGGPSILTVPLAENSSIKGPAVGRRAQPRRHPSDVRLPHHYDSLSFAADNSCGSDISLQEAPERGVLEAALMAMWEDRADRGILRYDVTQCPTRVLTGWHGFIVQLNEGRATRKRPTELTVDQVLQPFDPAKFNFTKAAITEVLVAFRPIVDGQRQPKAQAVAPLRGGGADNSNLVLVNVSPIDYGHVLLVPRVLDHLPQALSTETVLLAMQFARELGSPNFRVGYNSLGAYATINHLHFQSYYLAMQMPCETAATVPLPGALAAPPVPGSGEADAAACAAGALQISRKRPRDPSICMSAGSVHVSRLQGYPVNAFVVEAVEDHANCDGGGLEAVAAVVGTAAERLQAANQPFNILMSSEGRRVFLFPQCFAERQAAGVIPLDLLQTGVNPAAFEIAGHLLLKRAHDYEEATEDFAIRLLAQASLSEERFLAVANMCFGSGCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW28</accession>
    <name>A0ABQ5RW28_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Meckelin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004273</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019170">
        <property type="entry name" value="Meckelin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21274">
        <property type="entry name" value="MECKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21274:SF0">
        <property type="entry name" value="MECKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09773">
        <property type="entry name" value="Meckelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045474031" description="Meckelin" evidence="2">
        <location>
            <begin position="24"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="510"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="548"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="597"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="671"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="711"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="954"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="992" mass="110312" checksum="1B716C8BD227E851" modified="2025-10-08" version="1">MTAEVWFSLVVYLGLLKLQTSYAQTSLAIPLQTTCPVLNFFDISSLQCASCPNGAFPDKTQRACVSCDATSGEAYNFQSGYSMVQTWAPAVLQSAQVCACQAPPANSTAVVASAASNGTFFQRCVLCPTGTVANSLNQCAPADNKTSLPSTPAQQLQQALQQLNIGLTLSAAVQASISVPTSSGSSQLTLNIQQSRPLAELLGPSALTCKQGLGADRAACNAVANLCALTLYDSTSGACRMYDALVNLFVANSVGASPPPPSTAARRVTTNFKPETGSMPWLYYDGVGYTDDPHMDLRVQFSGGNVAEGQVSLLNFVLSSYLLNGTWLGYNMWTSEFQLCGAPKGDGSRWYRVGWNYKNSCSIKLEALLAEVAAAFTGAGDTVMHDLFLQVGTSTLYPIPVLISNMITETTVENSGAVRRFFFVDGRLGFSAQSQGLQAVQYAADMQLNIVLRRSPRDSIYVPQLTVTYSAWETNTLSTINLASDTINAQRPASFSVTYSNEPDLNSRFWYAWQTILIVFEVVLAFPAMMLSLFRYIRKKREQADLDFLVYIAISFADIGSFALAMVLLVVSLYYLILYKLQEEVYFLMLPDGDLENFKITVILAIVGQSLGLMWMLWRQLRTDVFFIDWEKARKVLSKEGTREEPAPVSCWRMLMVANEFNELQTVRVSYPAFTLLMMVMILEGANFIAAADVTPDASDYRNYYGVTTSIILRFGVEAFLFLVLFLGQYLFKRLLYYPYIANPVTQFVDLMFLANISTLIFDDMHSGYYIHGRNQAQHSDTTLRELNQELLKEEEGLTAQRGLVNSTANPKLADNQHFLMYITDAIRKTYDAKMLQLVQQATSDARNRKGVVNTFLRGSGRQRDAVLSAYQDLMLLFKQMIEDVERNQATQVSDPTYLQMILQLPPENAQNNPAFVHDYFSSFTTTIFIGNEVRLYVYEALFFCAIDMSLRNVALSALLTFFMVRVVSYLRAMFGEDNLSQKTLVDRHFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW36</accession>
    <name>A0ABQ5RW36_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutaredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="4">1.11.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glutaredoxin-dependent peroxiredoxin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004271</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[glutaredoxin]-dithiol + a hydroperoxide = [glutaredoxin]-disulfide + an alcohol + H2O</text>
            <dbReference type="Rhea" id="RHEA:62624"/>
            <dbReference type="Rhea" id="RHEA-COMP:10729"/>
            <dbReference type="Rhea" id="RHEA-COMP:10730"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.25" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02966">
        <property type="entry name" value="TlpA_like_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050553">
        <property type="entry name" value="Thioredoxin_ResA/DsbE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42852:SF18">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_47, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42852">
        <property type="entry name" value="THIOL:DISULFIDE INTERCHANGE PROTEIN DSBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="13"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="229" mass="24915" checksum="1DE0FD25EF1633C5" modified="2025-10-08" version="1" fragment="single">IITHCWQYWDRVMVVGTQCPLIRGLTYVKGGPLPIGVKSDALKRVFVIECWATWCGPCRTSIPHLTELQHKYKDKHVYVVGISSEQNLQVVKKFVDSMGSQMDYAVAVDTGGEVEEGLMMKAGARGIPHAFVVDAGNNITFSGHPMDPTFESVLRTAAAAASDRGAGAPTDRQALPLVIASLNELLEMPVKALKQILTERGLSTSDCVEKADLAKKIAATCSNVTYYKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWE0</accession>
    <name>A0ABQ5RWE0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046696707" evidence="1">
        <location>
            <begin position="19"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="203" mass="21246" checksum="EEFFA5CB267B8EF9" modified="2025-10-08" version="1">MNWIVLTEFGFLLPNVVGERVSCDDEANNVADLEATVRGEVRNRSLSLSLAPQYPVILVAGAAVNTQHAAVDDADPGDIGCPGGSGNDVAEWKTSQAQLCTGADDAEDEADDGCDGALKAPRSNLCCALEEREEVGSEIAAVGYEAEDTQTCFGGKSNDGGGEAGGVVRSRWRARAVARIPVPAPDQRQACKRAHSLVKHNPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWI6</accession>
    <name>A0ABQ5RWI6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine-threonine/tyrosine-protein kinase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="32586" checksum="FC8FC4A61EF893A8" modified="2025-10-08" version="1" fragment="single">TAMELLRQCWSDRPLSTEALRQLRSVDRLGGHLIPGLRLLARDVEGSAGQLRLLHEVTEGTRPAPEDADSATSPRVSDADAGTSYFQERQSILQSGGWGPNPRGLLTASEECRALGMSVGRMPVPAWLRLGHYKRIEVPEPIPVDSGYVVAVEQQLSALVVGPEADSRKTIPPPYPLIDLRESNLGGAGSAAGGGAPATAAARDGDGAAAAPTPLHLEMHRELADSWEEYHSAPAVEDYRLAAGSAMCIIDLAVRMQLECARVWTRGIVAQGSDGAMQCTRQVWWHRYRLWYRLPLRLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWK0</accession>
    <name>A0ABQ5RWK0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004588</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF289">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE VIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1314"/>
            <end position="1664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="879"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="994"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1138"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1182"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1221"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="879"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1154"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1222"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1249"/>
            <end position="1270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1673" mass="169376" checksum="7D54EC86AF4048BF" modified="2025-10-08" version="1">MAPLEGVNGGDLSIPARSPISMLSRLKYLCLCGAPAEQANAAPAGRPPYQALRKTGASGLSRDAAISNDTVESPIGFINSTVHSARGPATGSEDDATLACVDGQVLETKVDNPPGLLATMTFGAPPGSVASQRLSPTNLEVDTIATANVVSLYHYCHSSYAINAPTLSSPSANGQTSPQPETYIDLPKVSGSPTTLSPIPLGPPVGRMLRAPQPAAAGSVLADAVDAVGDELAMATTSPYTTAAVPRSRTLQTSDVSIQMGQAQEAVKTTTISIPSAASTTNTPSRVAGTQSPSSLPPSAPFGAQPPPYSSVPLPGPPETVRPLLSWTDQGRAAGWRFADNTSAPLPSLQLRRELAPLQGMEDTALLLQQYSQGTPPSAQELPNWQRSAAEVNGPLTSLAPQGGMPSMGTAINAAAVAAAATGAATGMVAAAAAAVATPFLVATAATGSRAASGTHHGVSGMLSYGYGNGGSGCGFLDASSLLADGRSAPYSGQISGTLSPRLASFQYSPGVNAAVISSYAQPPPSPPRSSVGLMVPSRVSLSGAGSVLSLPIETSSLFDVTAPRTGLLPEQTALEMSMQAPALDLWAAAAHVSLTSRIAQGAYGVVHRGLWHGCTCAVKLMLAAGTENLSSQLTEVFFCKALSCHPNIVQTFDCNVWRITADTLQSVRSFCTAAGASPFGAASPFTMAGGGSASASAAPMDSPLLPGAMFLHSMTSGWFGASPSALLSAAPSGGPSPSSHLGAYGLAGVAVPSSQASLDRHPGMLLGQGSTTGTAMEGASTAAAAAKVVYQGVPFSAAEPLVTAPGMDQVGSLASQTHAAGMPPPAAVIDGNTAVAVGAALPGLIGLQQRVWQEDAGAGTAGVGNLAVQAALPSSSLSGCLPSQSQASPQSPLKLPDAHQLESPPSDCMKAKGCCPTQLALSPGLQGKDAESELPSLIHIGEFPIASPLMPMTTSQPLPAGSVAPSANPSDLVALQPPRLSPRLRELVAGNVPAEATGDGSCSGCEQPPTPPRHPRPVSPPPQGTSDPFAQRVIQQLQQQQEGMWRQELEEQTRHAEGETMQKNRQIHAQSVNISQLRHGAEDNGSISRRHLAVNLAGALTSSDPEPAGPIASEAATANTALMPLEALLPSSVHHVPPHHGAAADSGHRNVIGSSPSSPRGAAAEAVATVSATDPLLFSAIGGTEGQTSQDLARRSPASGLHSASDRAFVPAAAIAARPPSGNTGHTLIESNWPPLDGVANHSPSPSPDLSSNPDLPSSNPPRGAGAATSAKAAASILFSHAVSRGSSGIPVNPQVAMSMGMGAGLAMSMGIGLCSSPVSSGVLPAILQPGSVSGTSRQLSERAMNLSIPGGPPGGGETGGGGLNVISPWPLQARSAVAMGDESDAAAAMAGARNLAQVLNLLGARDGQLAVVVVMEECDRGSLQQLLSKAAATVAATALASGGAVPASNLYAPFSGGGRYPLPAALRALALTAKEVALGMAFLHHNNIIHGDLKPGNVLLKSSRQDQRGFVVKISDFGLSRVLHSSGVTHQHLMATCTNGSSSGGGVSAGAVGETDGEFMGGALDGTVPYLAPELINNCQRSKASDVWAYGVLLWQLVTGHRPYDGLMQFQIMAAVSSGEQLLSWPSGAHPALVHLGRACLAYNPRDRPSFAQVVRRLQAFEAELRAEGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWM8</accession>
    <name>A0ABQ5RWM8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chlorophyllase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017395">
        <property type="entry name" value="Chlorophyllase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428:SF14">
        <property type="entry name" value="CARBOXYLESTERASE TYPE B DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428">
        <property type="entry name" value="CHLOROPHYLLASE-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07224">
        <property type="entry name" value="Chlorophyllase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="458"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="523"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="61487" checksum="26B3E5C960A14DCB" modified="2025-10-08" version="1" fragment="single">MINKNRLIRQQETAMQNQQYLRKYHNFNNSTYATHSGSVNPYRRPTSIIAAKVVGSPVSLVAGARFQLPLPQGHDFKWTRANWRRLAPRSLTNRGTTHLDGLQCAWLVSRRPARTGNALCRAQEAIDASSHSRSVAVSQYLVRGPYNISPPVQLRFLVGQDRSGALDCNVSVYLPQGAQQAGAPGWPMLVLSAGFLLHSGMYGSYAADLASWGLAVVLYDVPELVDDVTMVSVLGSILNACTSNVRVGPYVDARAILLAGHSRGGKLSVLAAANDPRVKGIALLDPVDVTNMTPTGPGYPSALPAMRIACGPPRNIPTLIVGAACNTGIIPSDANYSRFLASCPGPCWFLELNGAGHLQFLDAKVDLLSAFVQSGPTPDEAVRRVSKAAVISWALEMAVPLARGEAANIRQVLKRLKQTARLLEFQASLSWSFKGFDVVGSPTSSGAARSGNRATDRGGAAAAAGTSSSSHQSSTSSSFGGTPFPGVANNAQQTEPGREPQRGPGYYDTQPSASATQATSKTQGQRGDSVGGDSQSSGRGCGWSYEELIRMRARELKTILVERNVDCSDCFEKGDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXH0</accession>
    <name>A0ABQ5RXH0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAP" evidence="2">
        <location>
            <begin position="912"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="999" mass="107717" checksum="7086329342CBA84B" modified="2025-10-08" version="1">MILTELLPRCNIPQAEARITTTSTWLESQHVSDAARLHLISARRKYSCSCVAAAQPSAGTRDAKKRSPQAFPQIHRGQRSQRNQTNWQHHQSCNQSILDPRAKKLVEELGLLGWQCKSLRQSQLPPGNLESAGTLTAALLPYVPGLPAADLAHCCWALAQIGPAGARDPAVMKFVVAVQQELLASTTAVGDASALSSSRDEPAGGTCPGKGGGKELESPTGPLTHGTPGHDRSAAAVEPLPPRRDRRPRCAVRLSELDPKHISKLAWAVAKMGIRPTTAASAVRSTEVGGSLADAAAADGWRELQLHWWRELGLAAAAAIRRVQMPPQALCNTLWGFATCGQRHPELLEAAAQCIARGLRTRYGPQGVASLFWAFSSLGSANTNTWVRGVPAAGGGSFNDVYDQMYDMLIRLAAPLLDVMTMQHLSNVAWGLVMARLHVRQPAARRLMAAVCERATELTSAAAASAAAATAAVALPDDWPAAVPQPAVVLSNLRGLMIADVEEEEELGGRDGSFVRHLTHRTDLQWYDPQSMSVLAWSLAQVAPPGHRLYVSYLEAVVSVALSVRPGLVAFGDQDLAMLLMAHFHVRHYSAALVSGVRQEVLHRLQQREMDPQALTNICYCLATSNYYNEELYEAVATAAARSLERYGPALASRLVWSTSRARHYSPQLLAAFSNYIRPRLAECSPLDLVAAATALAQFHHYEPGLFRALAEEALSRTATPSAVAATAVAASTRHLASSPAATAAEGAAVAVAAAASQSAEVASAAGGGRRRLTLAGSRQLPPRALVNLLWSFAVLNHVDEALLAVLLERLRGLDVRMLGVPAVMQLCQTQVMLQDLYGNIIKPPEVLTQAQMELARATWQRVSSAATTVSRLQRDVVSCLQRLGTNPRCERVTLDGLFSIDTSLEWGGRRVAVEVDGPSHFTCSRPFRPLGRTLAKRRCLEMRGWRVLSVPGHEWRALGGHPEIEERYMRAALDGVLWRTHVLEGLSPATPSSPGVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXH3</accession>
    <name>A0ABQ5RXH3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004229</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44464">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44464:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="445"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="571"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="617"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="660"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="703"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1023"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1239"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1672"/>
            <end position="1701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1038"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1687"/>
            <end position="1697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1854" mass="189439" checksum="5CC52213F2510B5F" modified="2025-10-08" version="1">MRPHVCIPSGCQQWFSKTIAVGVDKFAYMSSLAVYVYSSITYLPLAILAKHEERLSALALSPFDSNLLACAISNQEIRVYDVDTELIVCRVSYPSPKGKATIIEWHRTNPGVLLLAIGSCVVAWDFTGAADAGAQGHANGSSHGRSGIGNVHSVSSANGVAAAPPPQPSSPPLSSRPQSIPRSMLMRLRNARGPGGDGGGEVTGRWVSLRDVSAPITAMDQCGGGGSSGGPGTAAGSTLIAVATADSRIFILELEPSGLAVRGELRTITLPDTAAAQSVFSGSGGPAAGLAGIGPPSTVCWEPLACNGLLLIVGRSGVMALYDTTTNEVVLSYGKQPATASRFAQFLPSQPGNFLLSSSRSGALQLWNVSQPQPQRLIKLGGGLVQCFALMKSTMATITNGAGAGNGNGDAAAMGKPRTAVLVSFSDGAIFSYDLDSQQVLWRQEGGHTETVFDCRFCTTDPNMFATASFDSSVRVWDVRTARCVKLLLGAEGILYSVSWSADGKLLAASNDAGVIYLYEYGRGVLIKALRQHTKQSLRVVFHPYKSTLLASASIDGSVLVYTVDGETVRALRHSSPVSCIAWSSLAPSLLASTSEEGELFVWDVSRPAHECLVKTLVKHNSRSFNVEFSPLLRNYLLSSSNDRTARVWDVSSGDCLVVLSGHTAEVRALAWHPEVAHLCFTGSWDASVRVWDVRTGNCIHVANDHHADVYGIACHPRRPFFMVTCSRDTTLRLWSILGLTPNLLPQALVRSISSLRAMGEEAAALPLSAAPIMSGQGSLELADRLGAVSCTPLERFAAISEFFLPPACIDVFWDIARYVRYGSGGGGGDSGGGGILGHLPVRQRPAAAATATEGCGGGGFAGVCGDDSSSLHHWTTAKMAMGSAANHLELAASSKARLGSVGSVRRDELLREAAQQHLLAGNVEHCCELLAEVGDWERALALAPVVGMALWQRLLKQRLQAMAEAETQGQVSDLLPLYLVSGQVPELVDMLSASCLYDTAFNVAAVQASGGYAGLNSGSNGGAHGGSGGGGRERCNSAGSPASTNGGDLLPPLQRPPSPNISSLMSQSVAAGLANLASLTAPLGLERVRSSTADLSSMTSATTGGIPVATFNGGSSGASSGLGGMPLPLPLPPSAGSSIAAAVSPLPPLMGRGGTLPSLNRSPSLRPPPLTPIQMSSAVAAVAAAAPSSPLSTHFFIASRPSSPPTNIGGSVGALAEAGIMPLPVPLASPAIVTVPSAQPAAPSSPRMPTPPSGSRLASSLTIRQSRCAAASGGGGGGGGGTAVADACGRSSREAFDRAVRVRCAQARGFLAQGLPLQAACCALTVDDVVGAVGLLLRGGQEEMALALVSGLTRPIATFQNAGGPAAAGGPDGGLASVAYGVGVESISISLGGGGGGGGTGTAGSGGTEAAAAVLRPQVLVAAACKREAAGDYLTAAELLKQVPSRTDLRDLLAGRCHAHADPTTAARVFRCLGLKEPQHYAAAAELPGALATDAAAGWEGVRRLLLAGQQAAAADRALAAVRRLVESGERVAVLSPEWQQAWGVINSLEASALGEATWARVFCHAAYLGALLALEQGYAGVGMYLVRVLEDWVIKQPDAELPVPQARLAAEVSEALDECLALPAYGSPGEVHEALLELVLAPPGSSWMQGMLQARLAKLESRLPLAHLSSTPTSAGVRSIRSPLQGGGGGSGGVEGLMSPSASSSSLLARRIGGTSFNNSVRLSSLELGASSGRFSIALSALQPAVAAAVAAGGGGGGVGGGGGSGAGGAATLVDLARGPVVVAAAHVPTRSSSSMIATGGLPMSAVSGVAIRGAAVQLDDRTTWLSLSEALALRRVMLYSPLGTGRLLLVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY80</accession>
    <name>A0ABQ5RY80_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004573</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF461">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="473"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="65"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="505" mass="53532" checksum="71E116BECDAD843D" modified="2025-10-08" version="1">MAAATPANLESYYAAHENESLAKPLLGDEAPINEQVSPESAELGHALDPRRSQGGHSCKAAAGRQCTTWQTTAALLTLQLGWGLWLIPCDFARLGWIPGLVTLFTLALSTAYSGTLFTRLYAAVPDAVLFGDIGAAAAGRKGRVLVYVTIYSLDATRCIILHLAATQSLYHAIAPLLEEALPLWQCSVIVGVVVLLLGQVRYLTQLSSFFMAGTVSQMIALAIVIYDLVVSARSSQEGSNSGTIPPGGVGGGLATGLVRHSHSPLIGDDFLNGNWAPVAMAVLNLIFAYGGQFAFLELITSMRSPGRFSDAVTLCTAIMTALYGGFGAVGYWSKGSSVHGIVIFNMDPSPLAQVAAGFIFLQALAQYLVNLNVWTHNLLVLISRRASRTTDDELWAEETHAKSTGDHSSSHWLAATAFVAAYSAVVAVAIPFFCTLVGFVTSVTYLTCAYTLPAWFAIKLLSGKLGGLEKAWLTSLIPVSIILSSIGLVASVKTYITEAYGGEGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYS0</accession>
    <name>A0ABQ5RYS0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="275" mass="28830" checksum="1A8B084B5241D80D" modified="2025-10-08" version="1">MGHADKLTNYPRNMLSCAKVSNRRRMRLESCTTCSYIGVGGCPGNSNMRRGGNGWKESSMQDYMSPFLCLGGSGDGRGGPGRHNFKGGSSGGGGNNNGRHSNFSGGSSDGFGDKLWSCSRPALLGAFVLFTVAGAFPGPSCATPGSLVNSSSNAFHIPSIGGSGKKSQLSSSAPASGGGGLSGSLERASMERSNISSTSISPTRRRSKAMHVFCKGSTLVVPLTGNDSKEVEIPLPRALLPWQRARIVEAPIELSEREARMLASRQNRVRDSLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYX9</accession>
    <name>A0ABQ5RYX9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="328" mass="35072" checksum="E3DE92F8F4D02CEB" modified="2025-10-08" version="1" fragment="single">KSLHIPATCRIYLSRRPNFCHYHIFFFLEPLSSLRSVLSSYLYSNLIDQLLTMQTISSRKRLREEKGSFSGKRCLRRDTADIMMDGASLVALAIMREARRSDNSDTSPAAENFDEDSPEVPAAGTVAGQKLGVPQYPLYGQRAVSWSGRPISVHPVSVAPSSRSLPRLGNLSMLELQQQLMLLGSLEQELLAELRSARREHDARVALLGGGLGPSNKIAANAVLNAQSTFHSQRGILQQQQEALARTPAFASASDAIPLSALAGSEALASSSREAMPPPPPRARRGPRQANLGRLARPETQDGVAAAQAAAAAGPTGTGSTGVVLGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZN0</accession>
    <name>A0ABQ5RZN0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005277</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF8">
        <property type="entry name" value="POC1 CENTRIOLAR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="70"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="154"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="196"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="238"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="280"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="387"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="495"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="396"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="561" mass="59591" checksum="4C45250EDA07FA59" modified="2025-10-08" version="1">MASGGARHGLAPLPAESQVEDPTLERSFRGHKDAVTSVCFNHNMKQLVSGSLDNCVMVWNFKPQLRAFRFAGHKAGVYSVAFSPVHALIASGSKDRTVRLWQPTVEGKSTVLKAHTGTVRGVAFSTDGRMLATCADDKTVKVWSVATQKFAFTLSGHQNWVRCCHISPDGRLAVSGGDDRTVRIWDLNSKRAVRVFEDQGGLINTVAFHPDGTCIASGSTDTSLKLWDLRSNVLLQHYRAHTGPVTHLSFHPTGSFLLSSSLDTTLKVWDLREGQLLYTLHGHEGATNGTAFSPAGDYFASCGADEQVMVWKTNFDRYLEDYTAAGIQKVPRGSEPLISDSTVSVAPAAPALVAGAGGLAPSRVAPFPSPAHIRAQPPAVIAATEGSVPAATQPSGARRVAGGAGRTVPAAAPSPPPPQAAAAAAAAAAAAALEEYEVEVPPPLNLADLPESLAATLQHMVGQLDVLTQTIALLDERLTRNEDYAQRLDSKLSTLLQQQQQPQQPDSNNPQVVMQQQQQPLYGGGRFVVAKLGAGAVGHGADCDESAGVMYDTVEQYSDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZV7</accession>
    <name>A0ABQ5RZV7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Flavin-containing monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_005918</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="860"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="905"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1087"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1239"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1299"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1376"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1455"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="917"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1094"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1108"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1377"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1474"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI63152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1522" mass="157343" checksum="7481CF0762CB8C1B" modified="2025-10-08" version="1" fragment="single">MPITIIIGAGISGLQCASAFLRLGHTVTVLEREEDVGGVWLRYTAFDVQVPREFFSFPDFACPPELEPEEYPTGSSLLRYIIAYAHKNQLYRHIVFRATVTRLHRLSGQWQCFFDLRTGGPGREAGQGMGGANGSGGGGGGGGGGGGVVVHQQRITADFVVVATGLHSSPHMPLFDGHHLFRGRILHAQDLPGSTADLRQLVSGRRVLLVGASKTAVDTATRVSAAGAEDTVVVYRQAHWPISRYPFHGIHFTQLLHSRLAASMLDPYYNAGVLGRAKGWLTRPLRRLFWRTLERSLIRQQHLSGDLLPTLPLDKDLLSGGLVHDGKWLQCLQDGTVRAIKGEPLRFAADGLVLRSGDFILADVVIMATGHVKDYSFIDPDILSKLGVQRDGLYLYRHVLPPLVRNIAFVGAANSLNNPLTSYLQIKWLVACLQQRVRLPEPHDMLKDLQRQTRWQRRKLTPQADRASMIGIYSHYYHDRLLRDMGLRTRRKRSLWRECFEPYTAADYQDLRENAESERRGQSTSAAAPEMSMALGSSFKLSAEPSAASPSELYDVTPAEHSVGFGFMPSMRRGAAPSRGSTATDTAAAAAAAVAAAGLTESEIGTAEAEARWVTMSGTAVHDSRPAGSATGYSTAPPILMPTLSYNSRTGVGQLVSTRLLTLYEQEALNGRAASAFGSGPAHGRLGVSSAVLTPPETPTNFVSPVSYGVGFRSVGRPALYVPPSSGRSCLMDAAALAMMDGVIGRALLDNPPKLSSSGAAAAAAAAAAAAGMATMTVTAAGQHPKRHVIAGASGELPALAAALSTASGLTGSNVAAAAVVTERARDEVHIGMARARTAPAATTMAAAVMVGARSERGPNVTGVTVSGRGVEPGATRASTSAVTMSASALASASGTVSAAVEVDRDQKELTSDEGPVTTSGPLSTDNDSSSAEAIAARVMPYNMEAAAAPGSNGGNGGGDGAKVRTLPHGLSASFMTPVLAAPAAMGSATVVYHNPVSDIPMSSLKEPSDGGGGGGGGGMGDGKVLHDEAEACGTPACLRAVDSDAGGFVPCRCDVTAAPGDDGGGAAPAVHATVAAAAKTSIQAASTRARLRSSGPPSAGSDAPGSFTESNAEIQGRTRSYSRTHSMADGRAAQDGSGGRFTPPPPAPRSLVRLVTTRHQQGTAAAATAAPDTLAADFTASGPSSVHAISVGAVRVRSSTAGALRASRRTSGLLNESDDFLGTPPSCFEGSMEALAKDHDRDDDGQGSRSRAAVMTWRQRRSQRYPYNGSDTDDYDDEDDDFDEYDDKYAYIGTMDTISAKPPASAPQGRRRAAVEDGGGGDGRPTFSSAATNVVATYAGGRTSGGPQGASSEPIAWALTDDEDGGSGVDVDGALDAGGGGVGGGKEQNEGPRGRATGGLSYKPTSLLKPSASFGATINRSTSPHDTEMSYRTSNAVGSRLASAAAVSAAAAAASGGGSRGGSTSFPDPRLTLGGGGSGGSGSNGGGGGTASSALERYAARMTAAPTFRMSLTNIADMKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S041</accession>
    <name>A0ABQ5S041_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="391" mass="41062" checksum="727794C347A5EA09" modified="2025-10-08" version="1" fragment="single">MARRWQEATWIESLDHLPGLNLMEKARARVIILSKPREDRELYLEGTYAEAAATIKALLKETRVGSFNPGPVSLPGGSGSTASRPAATPTASASIEANPVPRLDPVSATVLPIATKTNGGTCTAAAMTITSTNNTRSSSDGADDADPASSTAFKMGAINAEALSRVTAGPCATSRSLTNSKTPPAVAAIPSIVPFAEARPTGAPPARNSNLSTTSSGLRVRWANDQNSPPNPPQTHPSEIRHDCIAEDRSPSPPPSVPWWRSADPGASSMVGYKGCKGLDFASPSTSSSSSTTSSPRQSTAALNLAIYSLRTGISEAYDAVSRVRLNQPPIIFHSAADGTSQFGNAAQQQHRHQEHRKQLCKHYQTQEQKAIATACCGSPFAPAGAYNLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1P6</accession>
    <name>A0ABQ5S1P6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="2">
        <location>
            <begin position="91"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="278" mass="30660" checksum="373E436A480D3DC3" modified="2025-10-08" version="1">MGSSPGLGSGSGPGSGSGSGSGSGSEVAFPTPQPRTSGRARKPNSLLKDYVLLAGAKEVIEPKTLAEAQAREDWPHWKLAMDEEMRSLTENRTWDLEELPQGAKQIGLKWVFKLKSDAHGNVERYKAWLVAKGFTQKEGVDFGEVFALVGKYATLRALLAVVAEQDLELHQLDIKTAFLNGVLEEEVFTEQPPGYHLGDANMACHLRRSLYGLCQAPRAWYVRLRSVLEQIQACSLRMRMEKRSTSSCMWMIYLSPASQSAWLSALRHSCEGLLTCVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1Y5</accession>
    <name>A0ABQ5S1Y5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF948 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006436</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33825">
        <property type="entry name" value="CHITINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33825:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="39379" checksum="BF908D48BCD258F4" modified="2025-10-08" version="1">MAVNVACSGTLRWRALGRATCLLCPLHRRQLAALPLLGSPKRATVSRLRSRAPSRQLPSDETPSPTAAQTRRPITASPAGYIASKPSKRNHKSLSRCTRPVVITTAHHAAALIAATTTSSPIAASIGLPPAAIALISLLPSIGAFVLCLAAAALLLASVPAVWALARAAHQAERLMAAVESELPDTAASVRLAGLELTDCVQELGALGGELTRGVRSTAALATAAEAGVRGGVTAADSAVRNHVVPALAKVERDTRGVVEQHLITTAKLSYTRPLVAQAMAAARRVRMVLAVGRLAGGAVQTSSIPPCRLGTPHVTRSLNVRHHTSSDWTSRRYGGSWRLCGTSSESKIWSSTMTRNERSSGCCPSATGRTPKRARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S288</accession>
    <name>A0ABQ5S288_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase 4 regulatory subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006792</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP4R2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015267">
        <property type="entry name" value="PPP4R2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16487">
        <property type="entry name" value="PPP4R2-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16487:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09184">
        <property type="entry name" value="PPP4R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="465"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="54551" checksum="9B6AD7583EA39746" modified="2025-10-08" version="1">MATQAEPMDAAASTGNEPAEPERGGAPSESVAPSSAYISSEADLIAFTRLPLEERFLTSQLRGVLAESALTGRIRYKWPLLRPLVDFVLEQVLTAYDAETRVEVGPPGPDSVSETIERFQKLLSAFSDAPWTFQRLCEILLEPKRQYSRLSKLVLAIEKCLLVTTEQPPTDPDQLPPPPLCSSLGPVNTNPAPVYTNAHHGSHAGHGAGPSGSADDSAGFTNSLLDVRHHPPHHLHHHGNHVGTPEHIEEDHWPEGLPEGKGPFKAGGVGVGLHGSLRPVGGPAGSGHGAPSGAASTSGSGNGSPGGGAAVHDAHGHNQQGGGSGNPPVAGAAVVAPPVAASGSQAILNALGAVGTNCSGGATTVTSNASSANHRGVTSGTAAAPLADGTGLDAMDVDGTAGPARVSDRKAAGNVNAPCLKTDGSGHQHQQRLHAKGGIEDANRQGAEGPDGTLQSPVAVLKPVGGAAARGNDGEGAAQGGLEIGEGPSTEEQGPGEARVAGESGTDPGGALATVLLGERKGKVAEADVGTTPVAPVGIPPPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2D2</accession>
    <name>A0ABQ5S2D2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007176</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF408">
        <property type="entry name" value="OS09G0560450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="842"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="897"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="997"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="219"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="390"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="650"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="697"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="774"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1138"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1400"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="238"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="428"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="656"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="713"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="774"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1158"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1520" mass="154721" checksum="85276CC46A6193C8" modified="2025-10-08" version="1" fragment="single">MLQNKIDPWKGHRAESTHSRDSGSKGQVAGSSGSAHAPRHARSAQAPADGPGPASISPNVSVSVEEERSGARNEGNNGGKVLPHVDVPGRQTRRSPSASQTPTPAATSSRAPPRTGGVASVSPAASLKPSLKAGISRTVRATGRPADIEALAVHSGGVANRASHAASAPQRSPSPDLMPRAAKASITAPISPVSSLPVLPKLSMSSVTGTDAAMAATSITGASAPTRGASGSGSRSGSRGGSRGSVGRVGGGSRTSASAIATVTVTATGTTTGGGANASRPSAAATTTSGGPEAPGQRPAVLNTLQALREEAAGLLSAAGALAPGAGAGAGDRVAGSAPAFSGGGRQAPNSPSPSRLALPFLGGVGSNSMRASRGGLPFLPLFMMPASGTSSNPASPLRGAHPFLGHGGKNGRSPGRGRPRRPKRVAGQQQGSARLNLSSKTGRRIPDPTGSFEVITTAPGVIGEVQFSQEVWCMDLWKVLEGGCNQQRIWTFGTPAISNAAAAAAQIMAASGVMQTTAAPGGANGAAGGASGDGGGLGASAAAADGYTSAQPLALLNGVVFRAVAAGSHHAAAISSEGFLYLWGSDSRGQLGRGWPSTQHIAGHTLPREVQPPIEASLEDLQGALPVSLVQGIIASSTPRQQSSRRLMVLDDGGDSGGVGGGGGGEEAVGSAVGAEAASGASGSVGGGSGGTAGAMHLFPMPSVHSPGGGSASRSRLHSAASSYSSYRQQSDNRVLAAAASSVTAGLAIPGAATAAIMAAAAAAASAEGTSIGAALGPGPTPSVDPDGDPDDLGSPVGGSRRFNGPGEQPQQLHMYRLKMGVPVRVVACGALHTLAALDGGGVMGWGDNGSGQATGKPGVPAVRINTPTRIPEFEQTEVVCLSAGLQHSAAVTTSGSVWTWGSNRHGRLGTGLAVPVSIVDTLVTTPGRTSSHNDRDHFPNTAAMLAHAHHSPMGLHDDVQLGLTFLPTRAKFPAGVVVCRVACGYRHTLALDSEGGVWGWGSNATQSLGLADFVDRPAPVRVPGLPPCSAVSAATVSAAISMEGHLYLWGSDSYLNPWVHRNRVGGPQGAAMTPRSASLPLIHRVRWLGALRFRSVSVGARHATAVSSYGQMYWWGDEDMLPASMQYGSSRIMAKPPEPQDFLPIPRTEHIGDGSVPPPPPPPNDPRSPTSYFSGSSALPNRLSAFRVFTYRPVGRFSDGGSRSAALDSTVVSFSGGVATSPLPHESHSGSSFNGYLGLGGGPGRDEHDRYGLVVRLCVFNDRIYVSIRPRRPPPLILGSAGGMSYASRPTSVLGGRLTDGGSVAAMPMGTGAPGAVGSVGARSATPAGSAVGTTSIWNGDEQPVLVACGEGHTVCLCGQTRHQTTLDETRNAMMLMLRQYARHWHAVRRTAQARLLGGSPVRVRSRSPERRPSSGRSTRSVFGVNRAQLPLLQDVCKDPFPYIELDERPTDPRDELCTLDMVLVFCRQAGLIDNISEYNDVIELYRIQLTNKHARIPPSLKHAVELIGRDFSNNPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2L6</accession>
    <name>A0ABQ5S2L6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">beta-ketoacyl-[acyl-carrier-protein] synthase I</fullName>
            <ecNumber evidence="2">2.3.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007101</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.41" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF297">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="5">
        <location>
            <begin position="76"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI63989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="548" mass="57316" checksum="01D15A5CEEB1C92D" modified="2025-10-08" version="1">MHGWISHAIRFKRLPACPFHYKLPFTCLNHAKVTSQQMGIWFPLFEAMLPNTCRATSRLASPDPAYGFGPIPQLPTRRVVVTGIGLVTPLGVGVRETWRRLLAGESGLRQLQEVDFPESQRSALGSLPCRVAGLVPEIQLEEACNRMGLVEDVRRSSRFMVMALVAAEEALADARWRKGDVDVHLPDGGVRRRRVTGVVAGNGMSCTTEVAEAGALVCEGRGRRVSPFFVPRILPNMAAGAISIRHGLHGPNLAPSTACATGGHAVGDAFRIIQRGEADVMVAGASEACVDAISLVGFSRLKALSTGFNDEPERASRPFDAARDGFVMGEGAGMLVLEELEHALARGANIYAEVRGYGMSGDAHHITHPHPDGLGAVLCMQRALEGSGLTARDVAYVNAHATSTPTGDAIEQAAMLSVFRSGVAAATAATACKAVAAAPAGTAAGGPEAEGLSSWGTATAPRVAVSSTKGATGHLLGAAGAVEAAFTILALRDRRAPPTRNLERPDPSLLPGLVGPVSVQLPTDRPIAALSNSFGFGGTNSTLLFATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2T1</accession>
    <name>A0ABQ5S2T1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_006821</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052575">
        <property type="entry name" value="SSU_processome_comp_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046523">
        <property type="entry name" value="UTP20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011430">
        <property type="entry name" value="UTP20_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695:SF11">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20416">
        <property type="entry name" value="UTP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07539">
        <property type="entry name" value="UTP20_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 N-terminal" evidence="3">
        <location>
            <begin position="675"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 N-terminal" evidence="3">
        <location>
            <begin position="892"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 N-terminal" evidence="3">
        <location>
            <begin position="1239"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20" evidence="4">
        <location>
            <begin position="1848"/>
            <end position="2120"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="1">
        <location>
            <begin position="2420"/>
            <end position="2457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="572"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="944"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1015"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1058"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1202"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1302"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1762"/>
            <end position="1811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1975"/>
            <end position="2006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2675"/>
            <end position="2819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2966"/>
            <end position="3011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="572"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="599"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="623"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="961"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="981"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1207"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1773"/>
            <end position="1784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1791"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1802"/>
            <end position="1811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1975"/>
            <end position="1985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2675"/>
            <end position="2685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2693"/>
            <end position="2714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2730"/>
            <end position="2741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="2748"/>
            <end position="2765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2766"/>
            <end position="2780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2789"/>
            <end position="2799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="2800"/>
            <end position="2811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="2966"/>
            <end position="2977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2978"/>
            <end position="2987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3011" mass="320635" checksum="ACFF6912C38314DE" modified="2025-10-08" version="1">MKEPTSQTQMVTNQQQQQQQEQQPDFAGSALLLLRDLCYLLQPPQHLNADAVVRPAVGLPLLLTAQPHGVRLAAHVRTLATDWQPETGTDPRVSAEVWAAVSLLPHACESDFQAVAICKQVLEAASVALRNRPSGPRSTSRLKQVRGAGGGGAECAPVGGTVAAALDAEGGLLLIRATATELLAELLQRQQQSGLSVSLVKVAEASVQWMLDRLDERPSTDSSLGADYASLRAAGSLLSAVRTRTACGGGAAAAAAMGSGAIIEMARGLLSESRLRQMVGRLAPLLAHESQPLRAVVLRLLCCFDQPALEALGPDAKDPTATIKKQQKQQHGLSSAATISTGPRCDLLEVLYGINSQPFTIESGRKWAVAMSRLKTYLEYNRIPEFLVPAFAYGMVGVLYIRFSPLWQPASEALAACIQFQSQASWPIVFSFLKASQEELLSGDFPAATATSGRDGSGGGHCGRGRRRSAAAISAAITAAPVARELPPSQDPSLLEKLTDRYKTVVLSGTPQAGGGMTDPQNRLSHVLRAMAMAPYSMIEGYSKDWVPLFLYYTAARGSQLNDGEKDLEAAGDTAAAAATRPQKKARTREGAVAVKTHGSIDKDGGDNDEPALKERRESHGLAGCSSDAGATDAATARGGKRKLPKNRTAAAAGAAELPTVNAVSVLSRIGGKTWRAGLLDWLRLLADLRGVRGLHRWEELQRAVSSHLLEADSNIQVAVLRCLKPYRFRWLPAEMSDRLGRLADDATLREELVVFPLVSGADGGIGEESRPGLIPLVVRVLFPKMRKRSGRLGGRGAPGSARAAILNFLAGLRPEELRPLLELLLEPMEAAFTSPPPEAAAAVAPRVGAGATAAAVEELDRYRLLPPPWWSAPMLTCGLGWWLKAVNAAVLDALPARRKVGFLNSLEDLLSHLGHGLEPFLPTMLAITLRLLTTATAAITAAGGGGGVGKDEVPPASEPGMDEQLDEQDRDAAEASSPDVTDDDNEDVNVDDDVNDLNGANSFIDIDTARTGKMKNKKAFQGSRGGGKDAVTSSKQRIKKAEVEVEVEVEVEVEGRGVGDNDVSSDGDDDAAAAVAEPSDPGPMERHREIRSSALRLLAQVWLRFPASTSTDYNELWGVFLPAVQPLIPRLQFEAASSKEPPLLECVLALSLSPGLARVLGDMSTVPDETLSLVRPASAAASAATAIATATRGGCEAAAQAAADAMKQDSEEKGDPRVGDGSGEEAGRKEWQVQGMGSALIRGVMAMLSSDRCTEIVRVTVLDVLDAVLGCGERLLHRVLLPWLPDVLKALNVAISESWQRDSGGKRGSAGRGKIHRRGQSSTSHRELVILERLSAFVADSELARMLADTLVALLTQAAARSQRRRSSGKAAGRLDEAGITKALAVLAALWGRLRAGDLPDASVERYCEMMSWWAIRLTGREPRAQLAAAYGAVARLLPRMAVPAELLTELTAWSPTSLDDIDYDRRLAAYGRLTAVAWSAMDYVQALPLLLTALYDLRNANDLSLRQAAAAAVANLVTALSALESAAASSVDVTADDGDRVAATAVSIATADPPESLLRLTGRVLYPQIKSQLCTSSLAVRQEHLTLLRALAVALPRRFADMTQLLSHDDIETDFFNNIAHLQVHRRTRALTKLTKMLKAQGGGAAVTAETPVGLPPPPAPQPLRRCIMEIIVPLLQQFVEEGADGGGGGKKQSDIDKEANVTDAAVTALGAVAATLPWSQYEQLLNQFIRIMRTRPAKPHVRAVCAILDNFHFPLPDTEAGADGQPGGVATTATTAAATAARAQKRKQSTEGENLDKSTDDDDGDMGEDEVYSAAEEAAAVQRALLVRVLPALHEQLVERKRGEDEEVASVRAPVALALVKLLKLLPPAAEQAELPRALQGVTNLLRSRIQRVRDDARAVLVSMMAELGPRYLPYACHVLRASLPDRGFTAHVIGYTLHAVLEAVVNVSHAKNAAAAAAAAVAEPAGGDDAATAAAATPSGYDIDDDDGAGCAEDGKGEEEGGVGVLDDSLELCLPLLEADLFGEVSEAKEVTTFAVQYKEAKRCRANESYQLLASGITFSTHMKLLMTLVTDRLHLAGQPSIRAKLVQLLQFAARGVRANPTAGPKQVMALVVGIMAGCLAREEAARARAKAAVGPAFSAAAGLAPAAAAVAAAVVEAAAANRDVNVERAALHETLVLEFCLTLLHSGVRRGALTGRSSELMALLDPALPLLVRSLRSRNAGCVSLALRTLAHIVPLPLPSLHVAAPAAGKALNALLRKVPNIRHPIAQECFRLLAALLRDCEAYHPTTAQLRFLLRWAFEDLGEAGAQPTAFALLRALLSRRVVVPEVYDVMDRVQQIMVRSQSAPVRNACGVALLQFLLDFPLGPQRLKQHVGFLLANLEYEYESGRLQVLDMLAQVVAKFPVEVLQAHSDVLLMPLVVRLVTDTSAKARAATGDLLRALLSRLETSQLGKVVSYCRGWLERGGAGGGGGGGGGGAQDAALRRAAVQTLGFVAEVEGSKFGRRFLDVANGVLRILQRQVARGVDGDEDQSASLPDDESADADAACPGWQEAYYCLLLLEKMVAQASGALAWPTDSTAAASSLVPELWENIVRLLLHRHMWVRKAAARLVGYGLATPRVWGGLMVSQSGRAAQLALSFYIQLECEGADEATCTQAVKCLVSLSVHLHRNEANKHHNKEQIGGRVSQQDQREMREQQDQRKRAREDRGDGPHAPGSGDGTGKRARGAAAASNGSINGKDWRDGETEEEGEEGTEEREDGDEDAGKGEQAEGEAEGKEEAEEEAADTAVGAATDSNGADESDEEDGDGPDGQRQTFTLNGLVRRMVRLADDVRAERICQRLAALRWIAAAATALGAADVATYLPVLLRPLYRISDAAAASAAERHSGITTTAVARGGRVPEDVRQLAEEVMAHLRGLVGSEVLLASYNAAREYVRGVRAERKRRTAMHAMVDAAAAAAARRRNNLRQTARRKRKMEVLKRERSAKQLMQTGRRRGGGGGGGRGGKRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2Y9</accession>
    <name>A0ABQ5S2Y9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006951</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045139">
        <property type="entry name" value="Aladin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14494:SF0">
        <property type="entry name" value="ALADIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14494">
        <property type="entry name" value="ALADIN/ADRACALIN/AAAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="622" mass="64634" checksum="C1A35184CEB9C0C6" modified="2025-10-08" version="1">MAMHASPLPPEGTKTICENHYALIHVHNNTDVSGDPKMLPSVLLSDEASLELPLELLPQGPPVYPPDPSSEGGEGGISRAALYRWLVSQAQAQGLVSLVQQLGDLVAQSEPVRRLMALPLTPYIGKYAASLGPALRGTMQFAWNVLRRFKADPTPRAIAGPLEWHPRQPWLAAADRWGRVQVVDLGPSLQATGRDSRGARPEVLVSGPGNLRAVLAHEALQHQALCVSWSPTSPGLLAVGSTGLVGLWAMAGEARPPLAAAGRVTGPWLRLLKTAVAGARVTSLSWSPDGRMLAAASMNQAGLQVWDVATGTYVCVGPAAFDLVRWSPCGNYVFAAGHGSRQFYIFDALKWWWQRWDIEAGAGGAAAVAAGANVATARATSQAASTTRVPSDVVAAAWAPSSASRVPILLLALSGCKADLLALHLVEEPPSLQAQVLPVALPELHRVERGARVAGADLVGGSGVNSGGLSDTRSVSSPSVVDMAWDQSGARLAVLLGPAEMRGPHSVVLYSTSLAPLVQADLIGLARPSERLCRSASAAASATGDAVAADKTAPLQGEAERASIGNTDPASLVALPGALKAARLAPFSTVGGSGASDRAPGPFCVISARVGERGIYNLPCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S327</accession>
    <name>A0ABQ5S327_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="142" mass="16615" checksum="E5AAED80F9F09EA8" modified="2025-10-08" version="1">MFSFIYLSIYPSIHPSIYLSIYPSIHPSIHPSKQDPSRHILRRIPPVFKPSSLSASIYSLLHYKCRHALYLHVVSDNELLKRGSRSVTYSAGCGCMYLYMHVCLCLCTMRENPVLKILAYQRWQDRLLDRARQERRHSCMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S333</accession>
    <name>A0ABQ5S333_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="333" mass="37086" checksum="5E906836FEC992CA" modified="2025-10-08" version="1" fragment="single">MPIHQLDVSTAFLNGELEEELWMQQPPGYESADPEQACRLLRSIYGLKQAPRCWYMKLAAELDKLGFKPSVADPALFIKKDEKEIVYLLVHVDDIIITSEDEAMIRRVKTEIGQTFKIQDLGEAKVFLGMEISRGAHGEVKLSQRCYIEELLQRHQLVNAKPRKTPLPPGTRALPAEDNNKELVDNTKYRALVGELNYLATSTRPDIAQAMSVLARFMGNPSKVHMGLALGVLRYLAGTKELGLCYGGDGDLTMVGYSDSDWAGDPATRRSTTGYVFLLNGAAISWNSQLQRTSAASTVEAEYQATAAAVREALWLRKLTRDLGLRTQAIQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S413</accession>
    <name>A0ABQ5S413_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="102" mass="11176" checksum="5924CD526542D16D" modified="2025-10-08" version="1" fragment="single">LAVLASARASVSIVSRRADTYEQTRTRGTTLLRPRVGCCATTAQISVDPEVEALLSQYEDVFKDIPGLPPMRPVDHTIPLMPGAQPTARPMYRLSPLELDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S463</accession>
    <name>A0ABQ5S463_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008496">
        <property type="entry name" value="TMEM222/RTE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20921">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 222"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20921:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 222"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05608">
        <property type="entry name" value="RTE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="303" mass="32503" checksum="6ECB4EA21B41D968" modified="2025-10-08" version="1">MVAHTEGCSRSGGRPFALVWSPIPLISWLMPFIGHIGICSSTGRTYDFAGPFTVNEDALLFGRPTRYLSINPAQAAKAASGRYGGGLPNVTALGRLAAEHGATEGGLAVEVLQGAAPPMAFPAQDPPGRTGAEFAAAAAVAARPERVYSQSLHGGTRLGGDDGVCCGNPGLADSEQGLAAAAAAATAVRTWDHRLTVAATWYRLLNYNLMTNNCHCFVAHFLNQVGYRDGGWDMVNLAALIFIRGRYTSLVGLMNTWLPWLVVVCVGGYFGQLLFLYSYLAVCTPLLTWFLLYTWCCWRDLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4I6</accession>
    <name>A0ABQ5S4I6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_007587</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01372">
        <property type="entry name" value="KISc_KIF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="12"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="525"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="600"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="717"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="429"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="566"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="652"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="104"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI64348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1002" mass="107132" checksum="2371C16BA29C3F4A" modified="2025-10-08" version="1">MAEQEGGEPSSTIQVCVRVRPLNPRERDEGLRACVSFDEETKQVVLSAVDKNTLLQLRGATAKGYAFDRRYGQDITSDAIYDDCVAQLVEAVFKGYNATVLAYGQTGSGKTHTMAGGQGIHGVIEEGITPRVIRHIFALAEAIRKKEKPGEKTQIMAYGLELYNEDLRDLALAGGRDEGAKGWDGKGGSTSALKLQERPVGKDGRVVPEVVGVTEKVVSNGDDLLSFFNLCMENRSTSSTKLNDRSSRSHAIFTITVQRTIVEVLNELGADLKAKVRTSEFTSKLHLVDLAGSERVKRSGVTGKELKEATHINSGLLALGNVIVALAGDDTTGTGKAGPGEKKKSTHVPYRDSKLTRLLQDSLGGNSLTVLISCISPSEADFEETNNTLKYANRACKIKNQPLPNRFTTLEEDLLPVIPQAGMGASLGMMQLQALLEDHERLKEVRERRERERKEKEAKRAAALALLREEQMKAKGFQRLRATQYDELRRRLAVGAATSIPTDQVIGDELLKGFGTRLRLSNQMTNSVDSLGGGGIGSAGPASARRPNMVRPASSRSRVPPTTDESPSGTPSTSRPRPGTSTAKAVGKSSFAAWLASQEAGAGGEGGEGVASTADSEYGGTDDFVDGDGTDDYAAGEHGDESMFADEDPADGAGGDANGGDGSMFAEEDPAGKGEEGSAPAASKSKFRRAQKPGADGAGGPAVVGGSEASLAELDQGATSGNVEGVEPNESGGVEENAGAEDGGGYDLLDEGEVPKLLARFKVNQHDTLAVFKACTKEDFKDVISLLPENTPLPASDKMTYLLARLEVNPGRDMAAALLTVATGEAMMKEAVGPAVGCLMATFGTNMIASCIFDFDDPEDLRYYGVDERIELWGLQIPHTSVRPALAEGLTEEQALAAAEATKDNTITWSSVDRLNMSIGLLYALCNHAIENGIRCGFCVPRPQLLKRWMQAGVKMEQVKTSLTLIYPQTAHDYEYYRASTVAWFMVAEVKAKLERVLGIVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S529</accession>
    <name>A0ABQ5S529_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053334">
        <property type="entry name" value="Chloroplast_Sensor_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48206">
        <property type="entry name" value="CHLOROPLAST SENSOR KINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48206:SF1">
        <property type="entry name" value="CHLOROPLAST SENSOR KINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="426"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="902" mass="93347" checksum="A5B8B9348821756D" modified="2025-10-08" version="1">MVVAGFRIRTPQPCASPPCAQQDWTWPFQVVLLRVFGSRKKTHSRIFGGGLHASPSFERKAYALTSDTAAIPAAESVSADLNDMCTLQLGMLYQLLSRAGTSYSSMRCIMYARNFTSVGPGQVKLVRVAQFPVEPAGQGEEFQVQQQLLLHTEQSWALQGGSAAVDPSAMIEECLLHREVVALPSSSSLVFPLVDRGVLVGLLVVELETDRMNGTDGSSGGSNGGAEGSVGFPDQNGPSAALATAPQERGTCPLPTNLLRGLTDEELQCLRLAVPLLAKACGMDARASWQLAQSSATAATARGLLREVQRPLSTLNTFGKMLVPRLKDGDPDKDMANGILVQGKRLQDLMWQLEDALHGPTVAGSAGLGPSAAHSQFTPAALPGMPGSLTTSLSAPPFRSPSALPRDPNPAVQELLSLPQPRPAALLAASSSQRQQQRLEGPSPWVQDSPVKGSLQYRSIDAAIGPAELESASPRFVSSRGSAAVSPGRVTFVDRSLLSSPADAASSSSTRVPMTGVLAIRQSPLYTSNGQSSTEGVGQSTPGMRPGGRLGSNRSASTATIDVEMDGCDANGSGARCSSSKTGLLRSEYSPGSAQRAGEAVGAVDAACGWGPTHHQGAGGYMVAAVVSTNLATALAGVLTAACRLAAVSGIGFIVNSPLSAVLPRRSSAPPKSGKPRPHPSSIKDAGSSPIMSTSRSALLSNGCQVRASSVVSPCGDHSTPAASGPRLIPRPARPLLVGVSAAVMQKVVGYMLDIALQCTPRGGQVCVSARQDGAGVQVQVLHSGRMDLQRLHVRSSHMPRLLTAHDPRDAGAPALARPAVAVMPRPAVGMGKKGVRSGSSSGGGTHVGSGVLSVEMAQEIVQQAGGHLTVSHPFNMVNAQSGRLDVGTSVEIWLPGPGTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5I0</accession>
    <name>A0ABQ5S5I0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MDM20/NAA25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019183">
        <property type="entry name" value="NAA25_NatB_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF3">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 25, NATB AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09797">
        <property type="entry name" value="NatB_MDM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="708"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="798"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="964"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="419"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="807"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1216" mass="125112" checksum="5011246A5EE62D85" modified="2025-10-08" version="1">MQQMPKPISQIIFSGISVHLETWVVQRLPRQRRMSRRPKDPDAFERKVRPIYDALDSRNWKGALKLCQQALQKYSDNELIKVLKAIGLERSGKREEANQVVDEVIALNPIDEQVLRLAAIVLRASGRLSDITQMYENAATAMAPRGGELALVLQHEVFGAHVREQNFVKQQQVALRLSKAAASVAGHAAISSERYGWWVVISILLQARATLRMTATGVPAPAGQVPSVAMGPEKLLALAEGMMARQAAKDGRLEGYDALMVYVDVLLAQGKASDALSLVSGPLGASALRLPAERLQLRAVLSALSGDLPAASELLREGLRLNPDDWGALQLLLDCMLPGTAASVTRGSAPSCPPTFRPQHPLVLISGGLAEQLPPRGLAVTPDEACGTAAVIEAQAVLQELVDLVDLKEQSPSSRGTPGDVGPGAGGGNGYKAMTMRGPDLALVDLAMRRHRAAAAAAAAAATATDSNSSNGSGGGSGDIGDTEAAVIDAVLSYYRKYGSLVSCAVDLRTYVSQLGAGAASRLAEALETEADKATALASAAGESGGAAALTSLRRRVCAAQIRDDLGLPRLERCGEGVELSKELLDLYGTAQPLQAGLDERERGAADELPALAGAALMTAAGLATSDAAAVPYMLAAYGAFADAVRVRPFGAGMRISLAALAALLAAPVAAAAHMYKLDIKHIQLDTLGAQMLLTPLLAWPHAGTVSMADAESSPPPSSPNGDAQGNSQQLLHRALRDTNALFADHACDAGESIFTAYGHGMYTKVLEFTAFRERLAAAHTLAVVRAESGLADCFRSSSSGGGGGNSSSSANAAGADSGGAEAIPAVDAVRSASVAAAGKLNPDNLPTASSLRFNWDLSIRPSWLPPGEAGPSAAVLEWWRSRAAGEICGQGYGRCWWAATGSAEAPSSAAAEWRTCQASAAAHRWLLPHCIAGALGASGGGVLNLREALERLKGLMDEQLVKSSNDASTAGSEADGDSREGGATAGLASCTKIDAEALRRLDVTLYGAALAVRDCLGSGSASAAAVGGAEADAACRALGELVAAVRRLVSLAADELAAAAREAAAWGGVLPGGPLAAVSLLVQEHLSVAAPCIQSWQSVLKAVRKRRAKSGNQMEGAAQHLIDAVTKTAEQLAAAAQDCVDALTAATGAAAAGGEPLPAAAAAAQAVRFLKAQGHAAASVSESAASALEALVQEQRITVGLVMKRAAAVAAALLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7B3</accession>
    <name>A0ABQ5S7B3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009428</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="111"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="656"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="876"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="147"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="990" mass="105021" checksum="1C2F7C80B67B6044" modified="2025-10-08" version="1">MEQAQGSEHLDTSHVDWSFLPPTAMHVIGTLLPPSSLLKARLVNIHWASCLAALVDTLYIVPEYFLNEQTVTEVHKAFPKATTVNLDLQDTFMAVPQAVKDSLDRYTSQRNGQFSAAGTGPEDASSTSSEVCQTDGALESGRPSEDASGAAGAPTESAAEPPASPVAITAESSSQPSIAPPLAAPSALQMDSSIPASQPSQPYQQGVGARVAAFSVDFLRRSGTSGPEVSNGVTLAAPSSMSGISGAHGGGATAGLPVAGGPPGLAEAMPAGTTAAGGVGAGAGEAGAPLALPVPGAPAQSPVPPPTVPYSAISIEPLHSIVPSNATHLRVRLPDSLDVQAHHADVSSSWDYVSALLAHVGTGLSGLSLSTAPDAAHISMLSPLTQLTSLTMDEASHSTWSADHLPIVGSLTGLRNLAIKIPPFRSSRPEPAATPPPAALASWTALQRLTKLHVSALRYSFHSSLVAVLPHLTKLEYLALRAPQTRLPWPGTDKPLFANIGTLTGLTQLSLALGDHNMAPQDWQSMTTLTRLSAIELSVSEGSDGLNVPMGLEFPALKSLKLRYPNSQDLVRIAMWSGIQRLDIKFPEHEREWHQEDAVVELLDFRTKLLSTAGAAVICSVLDNAPAAHLTTSVALDSSGNLGHASVVALMGQMGASPQQQPGAGGTMPPTGAGNTAGLTSWWSPLLPLLALPNMVDFRCSAKEGLASHNPILFDEIVSGLCAAWPGVTRLCFKGCINVLSQPGGWDSLKQLTKLQQLELRHHPPQEGEPPADHNISRATRARFLRVEPIRLPATSLPDSLQTLLLENVELRRKETDCLINLSSLERRTTCRHFPEFVGMPLQRLVVSNCCVKAKGITSIASECKGLTHLVFAVYKVSEEADLKELQGALRQMVRNLTQLQVLKVRVLAGCITQDLVQDMVFYLPYLQRLKLTSSDGWADTALFLPLTRMSGLRKCSLAYFKSAEAARLLRAEFRTVLPYCKIKAKHEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7L4</accession>
    <name>A0ABQ5S7L4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="323" mass="35968" checksum="6D2E0C646232B8BB" modified="2025-10-08" version="1" fragment="single">LLRSIYGLKQAPRCWYMKLAAELDKLGFKPSVADPALFIKKDEKEIVYLLVHVDDIIITSEDEAMIRRVKTEIGQAFKIRDLGEEKVFLGMEISRGAHGEVKLSQRRYIEELLQRHQLVNAKPRKTPLPPGTRALPAEDNNKELVDNTKYRALVGELNYLATSTRPDIAQAMSVLARFMGNPSKVHMGLALGVLRYLAGTKELGLCYDGDGDLTMVGYSDSDWAGDPATRRSTTGYVFLLNGAAISWNSQLQRTSAASTVEAEYQATAAAVREALWLRKLTRDLGLRTQAIQIRTDSEGSMSLGNTPIMSVRSKHIDVQHHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7U2</accession>
    <name>A0ABQ5S7U2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="570"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="765"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="824"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="903"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="984"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="594"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="612"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="660"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="907"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1012"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1026"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1026" mass="105030" checksum="340C3381E9459B30" modified="2025-10-08" version="1" fragment="single">MSGDRALEPGKHATEAAPSQASGAAFADKAVSALTAKQTVDARESGLLMDKSIDGVAQIVNPGSEFAGFAESVRQSGTIGADAPIGPCDQRPGQGTSSAPQIAARSALASCPMKQSGLLQQDPASSAAPTGNDAQESGRPNLFPRAPAPSFARSAPARPTAGAASQPPPAVGATGLSGSRPPAPAAQGTAKNKQPSVANAGLAGRNRSRGHSAPGDEERGGSGGAGAAAYSSESDNPLDAGYKDDSSDEDYVDDDDDDEDYGVGARRRGRSTRPAAPTAAALTRRGAAVTAAAVATAGVAAATTGSERKKPKWAQPLIRPENDRVRSLRSSQPPPGPKSPPPAPPAGGGKSKTKSSHRSGDGKPKSKARSPRNGDGEAGTPGVDHRSAEEGAEGRRRSRSRGPSANCEGDEDESESGSGSGTDSGSESGSGSGSRSESDADDPASLIREVAEDAARIATMELTAMWEFAAVLEFLTTFRGVLCGHGPDAAPGGDAAATAAAAMLSSVLSDGSWSADLLADELVRCVGAPGSLLGRLHLGLLQGVARRSSRLDEATWPAVLNHRIATEYRMLHPGRHPRDYYKPTSPLPPPPPQQQQQQQQQPQGQGYGQGQGPEQQHGTATTEATEAGGKGKGSGLPVRPKRAAAAAGSGSAGASGSGRSGAAATVKGSPGSAAAGQGPAGGGGGGSGNGGKGSSGGVDPTLPFWAEKGREWRAYAVLHPRDRMLALKLLCDLRLEREDLKGAIDALVLAPRPGAAAAAAAAAKAGRGGGRGGGGRRRGADGKLPPAALTVDDVRKRPLGRDSSGAALWVVGRLPDLQMRLYREQPPSWPKPKKPRAASPPPAETKGGCGKGKAAAADGSGGRGAQKKKKGQKAPPPYLLYELPPEAVAGRWELLGMGAEGLGGAAEEMRRRSKKQQDRELADKISSELVSVLVDEAERRERKHKLEAKLRFTLGSDYYETRSRRTRKEVDYSTAAYDKMFDEALRGDRLPAAPGNTPGNNKRPRGGPSSEAADEGEDLGEQEMHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S850</accession>
    <name>A0ABQ5S850_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_009655</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03758">
        <property type="entry name" value="proteasome_beta_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035206">
        <property type="entry name" value="Proteasome_beta2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="193" mass="21343" checksum="60E61BC614B4AB29" modified="2025-10-08" version="1">MDFFVGIKGKDFAMVCSDTSATQSIISIKQDEDKIVPIDDHKVFCIAGEAGDRVNFSEYIIANVKLYALRNGTELNTKSVANYTRGELATALRSSPYHCNLMLAGYDKETGPALYWLDYLATLNKVNTGGTGYGSYFTLSLFDKMWHPELTVDEAVAMMEAGIAEVKKRLVVAPPHYIIKVVDKDGVRVVKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S875</accession>
    <name>A0ABQ5S875_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_009082</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFP11/STIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022783">
        <property type="entry name" value="GCFC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022159">
        <property type="entry name" value="STIP/TFIP11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045211">
        <property type="entry name" value="TFP11/STIP/Ntr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329:SF1">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07842">
        <property type="entry name" value="GCFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12457">
        <property type="entry name" value="TIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="G-patch" evidence="9">
        <location>
            <begin position="244"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="46"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="167"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="552"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="416"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="552"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI65527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="934" mass="101518" checksum="AACB1C5468CDE9CE" modified="2025-10-08" version="1">MANQNDFQHFERFDMENDYEGGQWINGEFYYSKKRQKRMQTEEERIYGIFAEDSDDSDSGRRGRRGDRDRERDYTKPVGFVSSGKIVQDTMKGDDGDGDEEGPLRAFGPAQRPGSVGPASEEEDTAERTNFAHAGLGLGSQGLGAGGGVGSSAAGFVSGGGTCGGLGFKPGASQGPSSSGRGGGGDSAQDDEDEEAVMPSAFGKRILEKAQQRLKKEQQAARSEAKRATTLNADPAFATFEKHTKGIGLKLLEKMGYKPGEGLGRNKQGIARPVEAQMRPKGMALGFGDRDEPRMELPQPGGSLRLGAQPGGRAGGADGAARDQDAKTVAADVGGPVKGAWKKKAKDARVRREYRTADQVLAEAAERPLQAQPILDMRGPQARVITNLEHLNEKAVTAEGDKTPMPELQHNLRLLVDLAEAAIQKLDAKLRHEQDTATLLAREKQRLEEEDTRQAKACERLAVVTAEVEAVRDLPASTPSGELAEAYGKLRSQYREEYIMYGLSQAALAQVLPRMQALLRGWEPLSEPTRGVAEMRTWKALLQSDAAGAGGPLGGSYSGDGTSGGAAGGNGSGSGSASDDPYTTLVWEVVLPPVRSAALNGWQPRDPEPLLKWLEAWEPLLPPSALGHILEMLVMPKLRRALGDWEPRQETVPIHAWLFPWLPYLGQALDELYPHIRHKLDVALQAWHPSDNSALVLLSPWQRVWGQADWDAFMAKAIVPKLALALGQMDINPAVQQMEPWMWAMAWKDAISAHLMASMLERVFFPRWHGVLQHWLTHGANFDEVTRWYLGWKSAVPQGILDHERVRAQFNAALNTMNSILEDGTLPPQPAFAAPYDVAGAYAAAAAAGAAAAAAAAASRDLTLRDLVMRFAEENCVEFQPKAGRTYEGLQVYSFGSVSIVLDNTSGVIRAQLGPRWAPVSLEQLQQLVVARRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S911</accession>
    <name>A0ABQ5S911_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">U2A'/phosphoprotein 32 family A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_009562</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the tilB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056496">
        <property type="entry name" value="CS_DNAAF11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003603">
        <property type="entry name" value="U2A'_phosphoprotein32A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23602">
        <property type="entry name" value="CS_DNAAF11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00446">
        <property type="entry name" value="LRRcap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="U2A'/phosphoprotein 32 family A C-terminal" evidence="10">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="231"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="373"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="377"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="414"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="455" mass="49626" checksum="1589D3EAD6ACE664" modified="2025-10-08" version="1">MGRITERLIRQRAEHNEGLVSTLEEVALHQQNIEKIELLGQLCPKLKILYLQNNLIGKIQNLHKLKELEYLNLAVNNVTKLQNLQRCESLKKLDLTINFVSKAGLLTVESLRANLHLEELYLLGNPCTDWHGYRQYVVAKLPQLKKLDGQAIKPSERIAAQQVLPELEAALVVELRAEGVDVEAAGAVEDDSLYDETGAAVAEVPETGYVDEKGELVRPWCAATRILEHREQEAANRDAEEKRREGTAKPSLDSGPPRPQRHEAFPEIADTDRIYQKNEGKWEFSLEEAPDGRSLVLEVAVGRFLDTSLIQTDLQPTFVRLLIKGKLLQLLLPCEVRPDAAKAQRNMTTGQLVITMPKEDPLKPVVDVAKLRPKVPGTSGTASGYPASSGPSSRESGAASAGASGGRGVRLPGGAMGTTKGAGNGAGDFTIKEVRRAAVTAVMGGEEDGDDPPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9V8</accession>
    <name>A0ABQ5S9V8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055325">
        <property type="entry name" value="CF161"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24274">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24274:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24569">
        <property type="entry name" value="CFAP161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="402" mass="42973" checksum="735B22F38175D0A8" modified="2025-10-08" version="1">MRDVTRFNPVCLIGNWAEDRELQRTILKDLLSRKGTGTLKLDAFRQRMASALAEVNLTRVADDPYVHFGDVVQLVHVDTGCVLAGDPADADTRPGEQACAATAAPDVRAPCCRNSLIILPYVPPKTATALEPSYSDSTVHYGQKVRLALHPGAWGDAADAGGGPHPMCLFSKPVSTTHASRYARQQLVGFTARVDSFECAWIVVTPDPNLRAASEGVEVAIGAPVLLVHCATQKPLCLEAARYPNDYGIELEVSGRSATVNGLKLSLEQLAQGVQKGFLPKGEQTDNFWTFVGGTKVEELPPARSSADEAAAFMDGLVTELSARQGALSLLERKLVTLENNHQLLPAEDFKLVLRQVGSQLPEDGIAALINRYAPGGKAGASIDAGLFRNDLRAAATAAGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAL7</accession>
    <name>A0ABQ5SAL7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fe/B12 periplasmic-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051030">
        <property type="entry name" value="Vitamin_B12-ABC_binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42860:SF3">
        <property type="entry name" value="FE_B12 PERIPLASMIC-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42860">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="344" mass="37199" checksum="66FC8B81CBDE933C" modified="2025-10-08" version="1" fragment="single">VLSLLPGATDTVRALGAANLLIGRTHECDWPELQTLLIVTSDKLGEMPPAELDAAMAACGSALPTLGWCGGGIALLDQGLSPYRTDVEQLMVLRPDVILTQMQGLGPDLTPDHYQSSLEQLLGYRPTVVQLEALEMEGVWRDMRSISEALKLGREGLDKIRTLQRRLKAASDSARGRPRPRVVVVQWSDPAFAAGGWVPQLVELAGARDVLGRVEAAATFTAQQLADARPDMLVFALCGFGLLESQRLATQVLEQLTAECRTAAEVLHRARVIVTDGRRVFSRPGPWLVQSLEVLVEALHGEAQEYGHEGQLWALLLREPAQDSVDVGLNLTRGSSVLTPVGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAR2</accession>
    <name>A0ABQ5SAR2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011086</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI66990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044669">
        <property type="entry name" value="YneE/VCCN1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281">
        <property type="entry name" value="UPF0187 PROTEIN YNEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281:SF19">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION CHANNEL-FORMING PROTEIN YNEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25539">
        <property type="entry name" value="Bestrophin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="642"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="642"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="672"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="697"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI66990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="734" mass="79162" checksum="F40C7DD550F2388A" modified="2025-10-08" version="1">MALKPGPSVPIDPETCRDGRDLENGRDLEVQPLVQHFSYDPNSRWHRLKQAIIEPPKSTVSIYADIPFNHAEWKRHRNAAWRHRPQPILFMRVAGGFWWQLTYTFIVSLCVGIYHHRVKDVTAASSKLSVPFNLTTFALSLLLVFRTNSSYSRWWEARTVWGAVVNLSRNFARQSMLWLPPKLARVAVRWMMAAPYLLKCHLRFNASVRENVSHILMPKELDWVLGWTHRPNAAAHVLATAVTEGRLDTNREKLLMELVDTFIDCVGKCERIFKTPIPAAYTRHTSRYLMIYLTACPLVLWPSLGWWTLIAAVLIAFLLLGTENIGVQIEEPFRVLPLDDMCRGIEATLREMERQHSRLGQQFNSWAGDPAALSRTSSESSLDSFIDGPADGPSEPAALDRFHNIPPPARLAAAAQALLLRRPSLQSGAGTVTGSPSAAAAAAAVTSGSVDISGRFTTANSGELLAMQHQQHYLFPSGTPPPHGLGAGGGAGSNLISGLLHHVMHSLGGSGGGSASSGGAGAGAAAPSPNILRNRSGSAPAAAAVSFSDSAELNPEYIGSGDGAANHSHNHMRFPHHVHHRHRNLPQHPADVPSTALAVGTVVCTGPVVLSPSGSSRFARVSFERAAPRLSVDEGRVATATTPFARCSQQRQGELGQRHECREPPPAPSPSALRQEQQQPEQMNEGGVQDGAVHGRTSGGSGGENGKSGDGDGIVNGSGSAAVAQGDVVSERER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBF0</accession>
    <name>A0ABQ5SBF0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_011168</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="10">
        <location>
            <begin position="106"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="302"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="383"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI67230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="421" mass="47249" checksum="72F96283FD384E1A" modified="2025-10-08" version="1">MGSISDCGGNSVSAPEEPPRKLLEELCAKFILTAPKDALNKDKLFFLVEQAWWYYEDKVRPTSSINLKHYSSYTTFAEPLFRKCEALSPLRPNLQAYLEDYRRYKQSIPVYGAILLDAAMEQVLLVRGNKSSMGWGFPRGKVNEGESESDCAIREVLEETGFDIRSQLRETDFLEVTADGKRHKLYIVTGLDPNTQEFEPHSKWEIGAYAWHRVDALPATSDEASQVYMSPDGVRHRFFMIHPFVGRLRKWIEKRRRLNLKGQQQPQSSWSTTSSAASSLQIPLRELQQVLSAPLLSPSIITGGAQHPEASPTSITQPVASPPYTAEQQQQGRREHKASRLAQSTWTTQAATPPQLHGQKEDVANPILQTLIQQVQNRLGELQVQQQQQSPPSFSPTGTGSTMSNFRFDRASILGCFAAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBT1</accession>
    <name>A0ABQ5SBT1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46699">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE STN8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46699:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE STN8, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="137"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="497" mass="55447" checksum="A87EB4042D2C103B" modified="2025-10-08" version="1">MQHQLPSRSGLRSASQHSREFKPCSFNFKNRQKALHRPVVSFQATGLELASIADAVQSVVTAAAAAAPASLRPAVSTIGGDIAALATLSPTLPGLGRLTALYYSLFSKPNPFWNIWDFYVGAPLTDNLNSRWSSEDFQLRDKLGGGNFGITFEGLRLSPEDQGVTTRSKLTAEQKKRRVVLKRVNMDRQGVRSDFLKTGTLAKGSAETGMVEAYMCAKIKRNPIAAASCAEYLGYFTSTTAEGAFTKGSQWLVWKFESDATLGDALDGKLGPFPSCLEEFMMAGKKISESTPQDKRDINVIKGVMRQVLTGLRRLHRLGIVHRDIKPENLLVTVDGQVKIIDFGAAVDMCTGINFNPLYGMLDPRYSPPEELVMPQNFPRAPAPFIAALLSPFAWIYGRPDLFDSYTAGVLLMQMCVPELRPVANIRLFNTELRQYDNDLNRWRMYRGQKYDFSLLDRNNKAGWDLACKLITKRDQYNRGRLSVGQALSHRFFMPEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBY8</accession>
    <name>A0ABQ5SBY8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_011363</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF83">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="420"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="607"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="145"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI67151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1067" mass="120302" checksum="3BB279037D229DFC" modified="2025-10-08" version="1">MHSSRGAAANAALEQQQQQRQARELVRKSATYGVMESDEDERQGEGKSAVAAREAIGPGRGSGAIGYGRSGTQAPPPQQQRKSAAADTTDGAGGGRGSGKHFRARRVQFTDGGEDKEDDDMVVPASSRPKRKWELEEEEDERQENEEQRQERLKEEARLRDQEEKAEFEERLRVKDEARTKKLAEREPRLSREDARREELKKRYEAEEENRDSLMPTLREVSRQEYLRKREEKKIQELEDELEDAKYLFAGVPLTQRELDDLKYKEEVLRLAKEKQRLMNQAAEDGYRMPDSFDEPARYTSRYDVAKLRYSEPEGAAARANPHAEQEAWEAEQMKKTHVTKVGAQDAGAAVPQYDYIFEDTMDFVKADMLAGNIEELFETAEQRRKKEAEARAAAAKSEREKIAVGRSQLPVYPYRDQLLKAVEEHQILIIVAETGAGKTTQVPQYLHEGGYSAAGKIGCTQPRRVAAMSVAARVAYEMGVKLGNEVGYSIRFEDCTSDKTILKYMTDGMLLREFLSEPDLASYSVMMIDEAHERTLHTDVLFGLVKDIARFRPDLKLLISSATLDAEKFSEYFDYAPIFKIPGRRYPVDIYFTKAPEADYIDAAVQAVMRIHVLEPDGDVLVFLTGQEEIEAMEEMLRQRMRGKGTQLAEMIICPIYANLPSELQAKIFEPTPPGARKIVLATNIAETSLTIDGIKYVVDPGFSKQNSYNPRTGMESLVVTPVSKASANQRAGRAGRTSAGKCFRLYTAHSYENEMEDNTVPEIQRTNLGNVVLMLKSLGIHDLMNFDFMDPPPAETLLRALEQLYALGALNDKGELTKLGRRMAEFPLDPLLAKTLLASETYKVSQQIATVCAMVSVGSSIFYRPKDKAVYADNAHRNFARGGVGDHMALLAVYDGWAESGFSTQWCYENYIQVRSMKRARDIRDQLVGLMERVEIEMVGDPTDIDAIKKAIASGFFYHTAQLRKDGSYRTVKHPMTVNLHPSSSLNPPRREGQPIIQALPRWVVYHELVLTSKAFMRTISEIQPQWLVEIAPHYYDRKELLDAEGAGKKVPKGAKARAAMDVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCC5</accession>
    <name>A0ABQ5SCC5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI66982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47650">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47650:SF2">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="425"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="670" mass="69646" checksum="894C95AB401EE794" modified="2025-10-08" version="1">MEGSHLRNHLSSHHVLQMKEELNAAVSELESALLEIKKARILQQFVAHNQPGANSGRLNPDPGPPPPLAAEAVLPAGPQLSISRALISLPAAGSSCQFRYMDGRWYAGRVLGPGRQAGTLSVAFEVPTRPFMLDPVDVMEALVRPCSDVVGAPGPAALAASRAGPSLSAAWATQQQMKQKQGHRQGQHLMPDGGSRRSFQGGLAVGRKALALLPESRLYVPVEVVALDVGRATATVLPLLQQEPHGLPGGSSGDGREVIVPLAYVTRHAHVSSPMEAGRGGDGEDGTESDGDGDDGSRDGSRSSSSVSALLRQPIEAEESCEEGDEREDFAGDGDGDGDGDEDEDMDMYGSDVGGGDTGADHDDDDGRSKTLGSFLRPHGARGGAPGGGADEGDGSLDVFGHARLAAAAGALDDAALLADWESHSRGVASRLLVRMGYVRGQGLGRRGQGTVAAPEVLLLPERKGLGAADKERVRRVGGDGGSGAGTKLHGGVEKRRSKRGGVRQKRKRAIIASREARREVREQEEGAAGGDGLFGFINSSLGDSSAAARIRKAQVSVAGIAAAAAAAPSTAGAAAIACAVAAASGGSRTQRPPDRQGLMTHHDNVAAIQTKMSRLQEMLGRHKDNRGLRAQIEAQIRIVQAELAAAQSAAARASKAISDKDVEKKWLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCM3</accession>
    <name>A0ABQ5SCM3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011743</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="201" mass="22776" checksum="0C6AB780EF389321" modified="2025-10-08" version="1" fragment="single">VVSGRQYTKVRRRLYQEGRTVCPGLIAYEELELDDVPINIDFNQLGLVIFDSQPLSSTNANAVVAAHGWKGALAFSQACRTLHDVEIDHQSQEIYMQVDPNETMLLVDYDKDDADIKLAVLASPSTADKGYDLTIRGQNVNKMSELFVPAVPKLGGRYHVSRLYELDDLQYGTRFEDIEIHRYHLSNPRQYTIQGLTRIMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCN9</accession>
    <name>A0ABQ5SCN9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011496</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF66">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="676"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="274"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="405"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="492"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1004"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1150"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="464"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="512"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="705"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1550" mass="158484" checksum="6A472F9E47B7756A" modified="2025-10-08" version="1" fragment="single">MAEKTAETAAAAAAAAAAATAEASSGARGTGVATADPAIMASGTHGSGPCSAPGLARDRPCSAPEWHRTRFQEGGDTGYSEANGCAAPVHTAAAVAVSAPVEERLEGVPTIRVLPQDPNNCSGAGRGCSSSGSSCGPASAASAAAPQNPTGSVGVGGGFNAMVAGGRVVVGRTTGRPPHLRASSLTSLLGAQGYQVMSPAAAMSAGTFRGPPAAAVNASTAWPSPTAAIGASGQAEAPPQVSIQQQVSFGPCNYSHQQQEPLTLCAVGTSVRTSVSQEAPVRATSANQEAPGKTATEDSNGTGAGAGGVGGSGVGVMESRHVTGWASSPQPPPPRKPALPPEARASSAAAARAAAAAAGAGSAVADTVTAADGAVVPVRRRPTSLRTAPTSQPRDLLHQVRRAASVDGMGSSSPIPHDEAPSPAGLHRRPRVNIAAPGATEHHPVEETARPGARVKKKSSAVGASGGACGAIKGSGGSTAAKALVGRKGSNRGAAAAAGGSGGGKVGKAGKRGFSATKAARAQAPAGAEGPAASAGTVGRSGAAAAVGTAAGAWGSTDNGRPAGLGVAGGLLLRRVAPDLSVIPEETGSASGSMGASEVGSGQMTQKIAPQGVDTQGAVNTSNDATFSGGLASSVMPAVAAALLSGSIPETPAFTRIILKQKARRGVPRELDAESFQLGRILGSGSFATVYLARQVDTGAKVVVKRLDRRPGGRTAQSEAENEVAVLNRAGSHPNLVEFRGWYRDPRDGVMCLVMGYCSGGTLAQLIKNPPASEAGRGPGGGGDAAGAFGTIVSAGGAGGSAALDGGALEADALAAAALQRASYFPEDVVMFWFVQLLLALHHLHGRKIMHRDLKPDNIFLASKRRVIKLGDLGVAKQLEGTFELAITCLGTPYYMSPECLASRPYTYASDIWSLGCVLYEMAARRTAFEALGLPQLMFKILRVAYDPLPPQFSRPFQQLVNSMLRADPDDRPTTQDLLSHPFVRRHLKRLMDISARKVTSRVPADRVSAMPPKVKASEARSAVKKLERASGGGKRRPGANAAAAAVDDLAAGAGLRPFSTGGVGGGGAGGRVNSPRAYGGAVATGAGAGCSGASGGGARVKNVLQWDSGARYEARRQQQVEMRFRAREFEQRITREQVRQQRRERDPAVRAREAELEALRQELLARRERKQAQVAEQEILEARLSEPHLILGDEMAAFRTRNKLRRTRLGLASPGAESPGFNFDEGEEADEHYFQDGDGVEIPEEYDFADNEGEDEEQYEDGEHNASGQSAVAGVGDDGGDCATAGDAVIPSNDGADSRVATMPSGCGPGDDAAGDSVGRTAETTVAVVGPEAEAEVPLISDIGQELIDQALLDSLAEEERQRASVLQNGSVEWQQALPSLTGRELYATTSSSARAVSMARSVSSASSRLVNAENGAAGGASMIITPRGDPAMDVTTQMHQHAVERQQEQQPHQVSGPLDEHGPYHHEQLLRLPGTLRKQHPRLRHFLQQQVLEATRKAASGDDEGCTDDEVLENLDAAEAASAQTWGTAPLGALALATGGEMDGGISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD67</accession>
    <name>A0ABQ5SD67_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04329">
        <property type="entry name" value="RNAP_II_Rpb7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04462">
        <property type="entry name" value="S1_RNAPII_Rpb7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="176" mass="19753" checksum="FDCAE8537E30CB0B" modified="2026-01-28" version="1">MFYYLTLHKSIDIHPKNFGPKLREVIREKLIAETEGTCTGKYGYVVAVTKVDDIGRGRIRQDQSGFATFDVTYGCIVCRPYKHEVLDAVVTSVNKMGFFAQAGPLQLFVTQHLIPEEFEFDASGDNSWTSMDQTTDIKAGTHVRVRIVGIKYDPTEVFCIATMKDDYLGVIGKDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDF3</accession>
    <name>A0ABQ5SDF3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAND domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012257</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20404">
        <property type="entry name" value="Tudor_Agenet_AtEML-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039776">
        <property type="entry name" value="Pds5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000770">
        <property type="entry name" value="SAND_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663">
        <property type="entry name" value="ANDROGEN INDUCED INHIBITOR OF PROLIFERATION AS3 / PDS5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663:SF0">
        <property type="entry name" value="PRECOCIOUS DISSOCIATION OF SISTERS 5, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01342">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63763">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50864">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAND" evidence="4">
        <location>
            <begin position="834"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="86"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="332"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="396"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="516"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="727"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1015"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1068"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="332"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="431"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="533"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="727"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="768"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1222" mass="126514" checksum="7CCA3D9C29FE1F56" modified="2025-10-08" version="1" fragment="single">MGANKRWKNSVRKLGMCNIGGGSGAGASGSTSSSAVTGWISLGRFMDINGIRCDQDALERSSTQPAPPPPAFVPEPIVATAVAQVDGRGATSSVGGSVAEEQPPPAQPPSAQPLTTQPPIAQPPAMGTSTPPHAQVVPVASYSQLGPRHRAVLFPHLPPNIPRIMPPELQGDEMVGHRIRVYWPLDKAYYSGTVTYFNAGSRWPYGVDYDDGDNENINLNKENWHLDERDRKPLPPALLERILTMSIAAVAANVHPGATVAVPGATAAGGLQLSAIPRHPTVAAPAPGLPSTSQSPDSFRRCAHHTQAATLQGEEENRQLINSPAIADVGGSAAAPAIPGSGPVSPLVGAARSPPPGRSLGQQPGAGSAMSSHQALAAFGAHLALALQTRPCEGVGQQQQQQQQRADVGNLVEGEGQGAGGSLVCGERQRGPPSQNQQQQSQLRASIKVQVLEPGQLSEPLRPLLGGATEIVEVKAGWREGREEVVHGGVAGAADLAIAAGNGSDDDASDMGMVDDRGTPTDPRVGAVVPRTLDSRSDPCRQQKQMLEQREQEGNNQELDPQRPHHRAAYGAVPLSDSTVRGGPEGTFGDATEADGPQGAAGSEMMGERLGAVVDTEVAEVIESVATGMAAKQHVVSARQEVAEAGLVRGLVLASADAAGDIYQRTGSATGAGGHVNLDGAFLINVDGGDSHSGDGDLGINESDIQSLLAEAADGAGQEMEDLELEKELPQDQGQKKRGQERDLETVLHVDDGTGRTATQDESEEPERDMPREDSELEARRDAEEDGAVGLAAGSEESDRAVQASGRQQGMAGGNKPGGCTPVATRGPCAGQAAATVAASGGLDGALLVVCNHVMGAFLLLHQLVACNCSECCDRTEGKRMCWTPIGFEEHAGMRSSKKWKNSIRLGVQRDASATAQGVTAGMALGEWLDRRGLVVTPRKERRRQIDAAEVDRCIQAPVTVNLTLLSVATKPAAARAAGPLAGAIIPVKGSGKNGGKTGGVDTAVSDKGVAMAKTALPPLPPLPPQSATTRKRGHNPVSVMAPGVDGSGGANGADADLQAAKRLKQVKSVQRRTGSGVGGEAAASLAGTRGPATAPGGLQRASMELGAVVGDAKTSKAGDVGTDQDSSGAEFGTSGYKVLRCSTPPGYEIFCLVPGYQIDEVSVRAYDSGKLIIRAQPHDIERAGLWGISPLCHIVQLPRAVRAESAQALMTLHGQLYIRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDG7</accession>
    <name>A0ABQ5SDG7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012111</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045041019" description="Right handed beta helix domain-containing protein" evidence="3">
        <location>
            <begin position="37"/>
            <end position="2657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1727"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1848"/>
            <end position="1873"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1912"/>
            <end position="1941"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1961"/>
            <end position="1977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1989"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2030"/>
            <end position="2051"/>
        </location>
    </feature>
    <feature type="domain" description="Right handed beta helix" evidence="4">
        <location>
            <begin position="423"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="829"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1198"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1432"/>
            <end position="1559"/>
        </location>
    </feature>
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        <location>
            <begin position="1590"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2218"/>
            <end position="2268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2335"/>
            <end position="2371"/>
        </location>
    </feature>
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        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1198"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1446"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1517"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1539"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1592"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1643"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2223"/>
            <end position="2233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2256"/>
            <end position="2268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2344"/>
            <end position="2357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2657" mass="283507" checksum="3CF3286B5CE7FE34" modified="2025-10-08" version="1">MQPSWRMYCTSFKPPRPKICVITLGLQMLMVLGAASDSIFTGSNDVLVPPTPHTYVDNSEQLRTALWWNYTSLLLAPKVYDLSSTLRLTSPVQLRGTVGQDGSRTVFDCGGGDRGALELLAGGFVITNITVRNCGGAAPILIDLRNSNNTRKGEISDCVFTGNAGYLAGAVAVMNDSSNVVDLKLRGCTFFNNVASKARGDYTYDYDYALGYVRRKVLGASALALGRGAYHISHCNFTGNLPVNTLYGIRDSTVSAPLVLTCWRGPAEFLLEDSVIKSNSGRQSGGIYFIGHPDTRTVSTGCTLTLRRIVIEDCWGYQSGAILASCVVKGSMCSLNLLDTQMRKNNANMSGIIMAREYGMSVAAVNLTMADNYGGAFGSVQVAAIFNESRFINNTGPTFAAMDVWGGLFTAQNTEFLFNMPLTINWFKSAAVRMSNSYGGRLLNCTFEGNTVHALLVYSAAVEVFNCTFRKNRAVAGSTFPYGAAMMLYGSTYHNIINHTVFEDNVALAGGAVMLSSTQDVSILNCVFQRNRAVSGGALEVDIPVVKVTVYNTQFINNTAVGLSETLFGAGGVVRYDAQNGSPAGNSSHTPDDYEYDYGSEVSDSQGKSELRVPVESCGTGGGGAACVRGATGSEVSFANVVFRGNKAYMGGGMYVATGSRCQQLVHCYPLKLGPNVTFTDNYADLAGGAVYWLYEKIMHTECARTDPSLVPPAWPGLLDDLQSHRFTTISQATIVASANITSAVAAIPEAVSQSPPQSNEVPLPAPGAAVGGDGGAARVAQRYMKHVMPCDSWSRNTVGRGGYGPHMATSAFFHLPMIRWAEDSPSVIAKSSSMPPPAAPSPAADSLLRPHRRQLQQRRKVLQNPTPSSVLVMPLVPPPPAPMFPSGDIIPVEVHIFDYYGQKVTLSMVDSPSSVLVTCTSLNALGQKAAEAVNGIANFTQLRVRDRVDMTYSLAFTARTAIREMGEVAANIGIRPCRINEELLATGDACAPCIEGFYAIPEQITISSRTEVASAASMASLQPSSDVGSSHTSCMSCPEGATCSSRHLGGVIVPRDGYWHSSLWSDNVMKCQYEDACMYSSRITDLAAFQTRHVNGEANGASNASEKYRAMECATGYKGNLCASCEEGYGRTSPYHCSRCRGMWVRLGFGLLVFGITFISIVATIRAALLSHPKDDGDLHLQSDLLLSVGVWDDTGPRSNTAGASNSNTPGEGGSINPFEDLGVPRGDNEQAVNGQTMARNCDVRSLGSPPEVELQGVVQAKDAASGVNGSVAAPLTAGAGASCGAATESPSDVTPEGCHEGIVVQRLRTLKQASSEQVLAILKVPSGQMASQSSVLLQSMATLSGRQSQASKQSYEAQQYLQHHASNESRWNHIRQNLGRAVGSNPAAVPADAPNCGSRPLLGMLPLPGKMNFTSVLAVVSKVHHDAAILGNGSGPSPHDAASQDLQQQPWQCSVQSGMSGFGGQGASPHGASPICSSLGRGATAAEEEPRPEDEWSGKEGPPSSINIKHSFLTEQRQQSEQQRGGVGSGSLRRRPSLASPASLLVQTHSEDQLKRGEINELPMPVFRNPRVKVAAADVQCESFSRVRAGDGGAATPRGGGDLGASEDSGTVQESELDWSQRGGAAATQEDDTAGAARALPRSRRTRIIRGRNRHKRNDVISEHATVMKILVSYLQVVSLVKNTPVYWPRTLVNYFNVASQASSAASSLGTLECSMRTNVLPKSVQMFMITILSPLYWSVALCLFWILRTAWLRYRGKLADTNTALSYLRTRCLISINCIIFIQYPSVVSQVFSLFSCMQIDTGPPMPVCVDPWDVATCTMDKLSVGAYWTQDLDQRCYRGIHLRLCLIVGVPGVLLFAAGIPLLSAWWLYLNRDRLEEDEEFYALYGTLYQEYEGEYFFWESIVMLRKFAITGVIVFAGIYRWQLTLLLALGVIAIALMAQAAYRPYISDTMDELERLGLVTTLVTFYLATYFMDDTLGSSVRVGLSFAILILNAITVFILVVAILRELYKAAIYKLVSMSDARGDLPLSFVVRIGLLYLGWRFRVIWTRFCIWLHCMAPLCFPLDLPHGSNSDNEGGSGGEGGLGGASNSAGRRAASAAELRCAWDQHHRVRWIQSVENLLNRVIIAAASGTPDRAGGDEGRLWRRIPSHVMQGVRHAAQSVNVLTARLSFQFSGGRLRSDRSFSSWRLSPDRVASVTAASPLLEGNKQQLQWRPLDLSSPQDGSTSQDPAGGRKQAASCHGYRHEDGKPPSGGATAAASSATATRPNIAAVTTTPMASEAPATDFQWLGTCILSAGAAGRARRLSNLQAHAVSLSATTVDLLQSQSAGPSILDQRGAARRMDSRVSDGDKPSPVHVPGLDAGPSASGRMDVTRAVQPTALPPVIVPAPAPAPAPAPAPAPAPAAAAAFGMFPSPFLSASMPVASCWEGRRGQRFHDIGVLTSGKAAARESCLRQSGRLTSPSLLKAMVLKAYGRSRGMGPRGDDKDAVTATACPAAVGTAAAPSLFIKEALRPASSADSARSARSTGGGDVFASAGASAFATTTASMTEPGGMSGEAAVACTIAPAGTQHGRAQERRTGAGRSAARLPLGFLSIQRWQQVEDVEMVVAAEECLEGEHEAREESGREEVIAGNGGLGAAWKRLPEVCVSMVEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDP7</accession>
    <name>A0ABQ5SDP7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14523" checksum="6A563C4E18DBC7D7" modified="2025-10-08" version="1" fragment="single">RASGSSSQLLAHLGTTTKNRDAQRRFRQRQREAFASMEARVRAQEQQLQSLYREKRMVEQHNEVLLRQLRAYEQNAEQQQQRQQQQEEEQKRQQARPPADTGHDDSYRPQLCCLCGSAIMQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE66</accession>
    <name>A0ABQ5SE66_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="114" mass="13104" checksum="68742D7940E0CA54" modified="2025-10-08" version="1" fragment="single">MLNAPVLKKLTQFINKTHREFGDWTLLRWDHRAGDTVLYNNLLHNVVTRTYKAAVKAEDDDEAMELHKRGIAVARLICERRPTAVVDFQSREAAMTPLEVLLEEGDTEVDDWLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE78</accession>
    <name>A0ABQ5SE78_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012581</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="542"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="760"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1060"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1154"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1239"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1326"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="786"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1161"/>
            <end position="1171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1438" mass="149112" checksum="4F780FF76AC62EFB" modified="2025-10-08" version="1">MATLNEGLSLVDDIARELSFYDGGNYKQITEQTIINRCKDLLEKVKTNIMAKDPDGIIRAEKLSSDDWRRISLLVGPLARCFTASSPPAAPTVKTRHASAVAVAALALLSSCNQQSKVIATSALENGVVDAFVSTLRNSLRSQVNDRVVKAMLYKLIESLWGFTLYATTQLQQDMVTKDVVNVCLDLVTSATVEIESKLNASKTLYNCLSSHGRVVTELRASKVAALNHLVQQLGSQVPSPVAAAHLTPTGPSSGTSKTISMPQMLPSIMSWIWFLPSYIVDHLIESLAFILNRVVVLVWPEGHSGIPLLEQGREEPSAGPLHVAQLSLPNRSLQKTLQNLFRSIASGIADKRLVSILRGARLPSVMHSPAHSEDLEGAEAEEEAGSTGLPARLVKVVREGRPDGNETRVALELLDELFACDANGGHPELADDALAAGLLASMTEMGVLHSLHTEAGIAGVSAYVRLTLKLIRRVPYTPGLGSRLHGSGVPASVAYVAFWPTSRVSLKLEALQTLYELVQLDTDMTEHVVSMNNFPVALTNTLLGGDPDTRKPAKKTGGSSSKKKSGSGAGTAVAAAAAAAAAAATSTAVASEISVTNRRDHRDQMQETAFNILTVVLDEARESDKVWETVVESGMLPRLVQHSCAFPMQPALLAELLLAIGITFKDHQDDIASVGPLQAACAPHILPLLIHEEPVVVAAAARVLRDILIDMARYRSDSTDVGEGDEIFKALVAAGPSIDTADVAAAKAAAAAAAAAAVVKEGEPRPSPTKPGLVPCKEEGGSPNGGPVVPPGVSGTPVDESQRSSAQGDGMMEVDTGPISGAAPSTVVAGFTSAAGAGDGSGPDGGGAGTSVTILDISDTAVVAAATVAGDSGREPDTLELVDVTAQGLGLMCAAIQTCMATVLQRPRGVTPASASSGLRTAAVATMQVSSTAMSPTKGGGGAVQTAVLTAPPMVVEGPAYEALGALLLGCRLLGEACLHMPPHGNVRKHLARAFAYARPGAEELAVVLEALPDIAHGHLQLLQDCRALLEVLGKIPVLNVALLPKPKVVEEEDANLISAPVPLPGSKKGRPTGAGKRASQLGLAVDGTLQPTQGPTKRQRTASAAALAAAEDTAPRTRVSALATNGELTATGSTPVPRGAAAAAGAVAWNGSGLPPEVHSTTGTISQNGKAGERTSMEGADAAGGGSQAQYESEGPGTGARGSGLPSSSAAVAVQGSGAASGLGIPLPVVPRTMRASQPERVIGQEQAAAQQPAPLPESPSMSLENQDCQAGPSLAIRTAVMAGGVDAKQESTVAGSAPSIVNGVPDAGGTKGRVNAAEFRARYLEMKTQNARMIQELENLRLAKEESEALQRKTAAMLSSRNELLARALQELGDKHQEVEDLRKQLENMRAELQGAQQTVNSLQSAMTGSAAELEQLRAENADLKSELANLADLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEZ2</accession>
    <name>A0ABQ5SEZ2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="138" mass="14176" checksum="1CE9B667E80679D4" modified="2025-10-08" version="1" fragment="single">MCAVGKNTRRCIWGNLQCVLATDAARGPRHILPCFPRAVEGGVRVLAHISRGTGAGAGVGAVCGTPERVAVDGDDDKDGDDIGGRGTAMATWPLTVTGTAIMVTLTGRDGDGDGDYYSDGYGDREGEGESVTLTADMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFT9</accession>
    <name>A0ABQ5SFT9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056843">
        <property type="entry name" value="THADA-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25150">
        <property type="entry name" value="TPR_Trm732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region" evidence="1">
        <location>
            <begin position="137"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="378" mass="39343" checksum="25C836C673B7BBB8" modified="2025-10-08" version="1" fragment="single">QLQEAFQLLLEVLACQDQYAPGVLPPLEQFKRQVVMQLLAVPATRKGRYVSLGFMAERGCALEMLRDHPSLIPETLLAMQCDTTASAASSFLKTLLAAARTEANASQELALEAAAACGASSAGAGASDTALAASSPWVALWRGALLRVLYGKNEKLRAYVSVHALPVVLDLEPACALELIHATLVDPAPEPVPGTSRVAALVAVLKAARGMGLVADLDGLMGRAGRRHLEMDPEALLTAAVGHASEAVRLDALELACISSKMSDPPGQLELSIAGRWLSLCMRSTAQGSRNKCLVLVTKLIARIRMAVAHIHNKMASRGGPAHPSSSKGKGVQVIFCLDTNDEADAPEAGAAAAAAAAAAAAAATADPWSAETAILKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG65</accession>
    <name>A0ABQ5SG65_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46873">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46873:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14295">
        <property type="entry name" value="PAN_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Apple" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="1">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="341" mass="37212" checksum="ADB512F445A05EB9" modified="2025-10-08" version="1">MAPAYSRSGPLHAMRIRLGTILTGFTLLLAGVAVVVVYRTTFINAPFLKGSKLSELDKTGSTALLKTLAVAGMQCRVQWGVELWGDALVWGDLHHTESEAECCAACYEHHSTAARGGLDKGTNSSTCNTWVFCGDPQRCGHRYGECWLKHHPALPPADAPLADGNTMWNSGIVYDDVGLAQAYEKYLKLTMHTKLGDIVVTLMPELAPNVVRELRREVLVIAQRGNGCGNCKFYRAEDFGIQGVILNPGCYIGTPDKGHPNGADPKRKIPPGYVCRAGMAGSPHFFINFFEADWGDALCWGRVDDLSLAKVISKRPIKVKATPNELSMLADELSFKMTLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH37</accession>
    <name>A0ABQ5SH37_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone deacetylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09992">
        <property type="entry name" value="HDAC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF25">
        <property type="entry name" value="HISTONE DEACETYLASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="2">
        <location>
            <begin position="844"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="909"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1179"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="911"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1210" mass="125133" checksum="77E026627E1C254D" modified="2025-10-08" version="1" fragment="single">MVFGSSAIPLHVRYHHAGSPYEPALATNASTLHPGGFECSTPASPSRNSDSHAFAQLAGTNAAHLDGTASPSPSSDSAAVGGAGVAATPLADRPALGRFSASFPQFGAHSMSPLDPVSASVIYSDNARGAAVMTGFPVMPYEQATVSGNEHLMVSVHLFQREELLQGSVPDSAAAAADAVLLAAAQSTPRQESCFGEKDAAESGRFPTQSVSPPQPHCIAIPIATGRPSTPQPQQYTPHGMGAPAAAFPTRPRHGSATTISYGLSGCHPAGHGENAVVGLGTSPTGSLSDHITRTASLPLLMCNAVIPAGTSPAIPIAIAGVGASSRTRATTIHTPLPSSGGGSASTAAQRSITQNVDHQFNRQHFVGHHQQQPIYLQTDGKARISSPVQFVGSVSPSAASFHSTFPGLSLAAGGGGGGSSGEGGTANGEEVCLPQRQMQLHTEVDGGAAPAAAAVCGGRGPIGHVGAGTTRGSDEAGTQFNLMEGESSPTSGGCEAVGGVNCRSGAVKADTTTVMAAEAAARDTDGDEEFEDAVEVQHDDGDHVGFHDRVIMRGDTAVPPPPPSTAVYPADQHHRLQRCNYSSVEPLGLDTAILGDVVRGGGSEGRHPHGLAEKLDHDAQHSAPLASLGTGSAGVSPMVGYHRRPFDMDNSGDRILMQMGAQGRGHGEEEHGEIDGASMVDALDEEDVLDGDFGEDDVLEGEDEDLLDGEEEEDGGAMVFGSAAMTGFTVSVPTVPAAPAPAPALYPSAYPHEQHFCVNCTRPFWGTLCRFCRHPVDTEVVVPELLEGAVQSMLAGQQQQQQQRHGSGGGSRGEGVLLAYDDRCRAHLEETSPGLSHDQGRSHPERPERVAAIMTRLHGAGLLSRFDRITGREATLQELVAVHDVDLVSELNARTEEAARMAQVAAARQGAGGPAAGPGGDDSDMGEEWSMPQRSAHVLDCYFNSSTAVCARLAAGSAAEVARRVVSGSARHGAAIIRPPGHHAESSVAMGFCYYNNAAVAARAAQAAGARRVLIMDWDVHHGNGTQRIFYDDASVMYMSTHRYDHSCFYPGTGDATETGSGPGVGFTVNVPWNGGGVHDEDMLAAFRYVIRPLAAEFRPDIVIVSAGFDAAEGDPLGGCRISTAGFSHFAAMLSALAPTVMLLEGGYNLIATAAATEACLRVLLGEQPLPLQERLQARAGATSPQPQPLDPAADPWIMSGLEGVSDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH42</accession>
    <name>A0ABQ5SH42_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="144" mass="16457" checksum="DE272D449F9E0CB5" modified="2025-10-08" version="1">MSNKWWENSRTTTGRGIFSQYNPDSVKEWVERERKLPRQPPGPCPTLEQLRTLQKPPPTPDGFTTREPRPTTAPVEQPLKSPTLPQYSHHHHFGWLPPTPERRIGGSFVDQKPADSGHAYSYGIANGHNQRIHNTHVLISTRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHL2</accession>
    <name>A0ABQ5SHL2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein arginine methyltransferase NDUFAF7</fullName>
            <ecNumber evidence="7">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013949</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NDUFAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF7">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="118"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="213"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="268"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="295"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="551" mass="55465" checksum="30CC97E98B62ABB7" modified="2025-10-08" version="1" fragment="single">CVHSWMTMGRPPRLQLVELGPGRGTLMADLLRGTAAFRDFASSLEVHLVEISPALRALQWAALRCSSSSSNSSASASISPNGNRGGSGDVAVASEMQFLRRGVAPTLAGRAEGAAAALSTQLSSAPSPPSSSSSSSSERAIRDRSAAVAPSTPDCGLSALSGCLVHWHASLDAVPDGPVPALYIAHEFFDALPVHQFIRDSKRGWLEKMVDVQREDDTNNGGGGGGGGGGGARDDGNNNLDLDLDRNRNPSSERNSSSPNPRAVVTLASFSPPATATGTESSASVGKDAADMRTGDSASSGSGSGDSDGVCSSWHPNTSSLSNTSSSGGTAPAAPCSPAGSGLRLVLSPGPTPAAALLVPRRLGGLPGDQAQALDALEVSAVGMATAERLAHRIGRHGGAALVVDYGREGPPYGDSLMAIRAHRGVGILDGPGSADLSAWVDFGALKMAAEGAASAAAAAAATAVAASTPVSPPPSPVRTAGPVSQAAFLRGLGIETRLKWLASRASNLAEAEALAAGCARLLGGSEEGGMGVSYQVMAIHQADLREMAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHT4</accession>
    <name>A0ABQ5SHT4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_014141</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF7">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="7"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="172"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="586"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="647"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="697"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="978"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1016"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1288"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="593"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="697"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1022"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1288"/>
            <end position="1305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI69507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2224" mass="230792" checksum="19E82D32F15FD095" modified="2025-10-08" version="1">MNAPPQWALCAFCGQDRKAYSGSLGAFLGPLDDGNRSGVGVYVHRDCALWSSEVYHSSDGTKLRKVGQAIRRGRQLRCGHCGERGASLGCRLERCNVSYHLPCARASGCAFFTNRFLIACPRHMAEFKHELPPEGRGQEQGRGPGDKGGAATRRPSPCPWLLRISFGGHLRRQAAHEGPDLRQSNPSPIRPTARHLQGHTQPCRGGGGAEFNGQQDQRAGAGGLPSKRRKIDHDAVARHMAVAHAALASARCATAAAAAAAATEVDDDEAVFAVKERRRHAKDIGKLAPVILGRRAAAGGSDNGDGGGRGLYSRGFECVGGLQAVIQSLREMVLLPLLHPGLLEKLGVAGAAPPRGILLHGLPGTGKTLVVRALAGEVARTSPVPVALFARGGADVLGKYHGDAERTLRLLFEEARRRAPSIIFLDELDALAPRRSISAGGADQIYASVVATLLALMDGVADRGQVVVIGATNRPDNLDPALRRPGRFDREVAMGLPSREDRAAVLRVHTVGWRPQPSEDTIERIAEATEGFAGADLAALCCSAVMAALRRTAVSAAATAATALVSPEPASEDVVEFLLHTREREHQAEGVRQCAQQTHGPTQQQQQQQQRKSQQPECGDQVGAPGLPKARAGELPVGRLDAAKEEVAPVGTEGPASVMAAHGGAPTASAPSDIEQLNCRARSSDGRDVCMVSEKEGDMNSDREVQGKGSTATGYGFDSQLRPPVVAVSMPVADGRLSEPVRCKVQAAGSHGRRTNHSPVLAQAVCKRFQEAADVEQNSANVEGVGGNRSSAEVGTGHHTAAISGGAATLPRQKKSFVSILRCVGEMFDGRTMPVKNADAQDAMATDVVMMKPQTTAEAVSSAAAIRTTAARADANTGVTKDVVPAATEAHDYRSHDVDATATRQLTMNPAEWRGWERAVDGGPDQAAAGVAGIAEGATTFCSPTSADDMDLEAARTIAAATAEPQRRSAQTCFARNPQQVQGPAVHMTDLGGNGSSATPLDGRNHAHQAGAAVLGQIGSRNDLHADGETLKGGRDAKEGSEAEGKENDRQDPIDDLLAGVRVEDADWELALAAAPQPCSRRDAAAVVNAAGPPPAPPAHTAALVAPAVAVALLWLMSTGIAVPEPILATATAVVAATSWQPDAGPAAGSVVESAMGSSDTSDPFSVITAFLRLLISVGAVEGPAPATPNHDNVDYGSHAAVGNGPKGNVPGVTGAAPTLVELSHAGSGHTALGGSARLPRVLHHQPFSLPHTQPQLSACHLLLCGRGDMGQDAVAALVLRLLSAPHSLPRQSPQQPPGGHQPLRNAGPFGLLGSSGAAAVHTISLPAIVLRAGGGDCIEDTAAGVSSLASEALSPTHGSHGQLQVLYLPCIDTWALEQRIQINKDWDSDDGDADAATEPEIQGCEDAIGDEAEIDGDEADAMAAAAVAAGAARDSGRCDLTIRAGKSQKPQQQQQHWLEDAELPHADDDDKDDDDAIEAEATLRSRTCISPAWSVFMQQVSAASIALGRPNLNRELRGPSGFLVLATCAVPPDQLPPEILEFFGTQQSQGQPERAEAAATAAAMTGWAAGASWPVGGTKPYGVQQSRPTCRQTLTLLPPLRAAVEAAEAAAEAAVLPTIRRVLENCVLQALRDAATVTHDTAADDCVSGGEVGGHKPVARVAPAGVGRTQHLEAAGSLEQGGRCGINNMESGFLSPIDGSVSLSLNATELERGRRLCRQVKIAIRCIASSMLRRGSGCHLAQAIVVQQLSCGNGATAPSSRAILGAADIANGESHLKGDVVELRLCGGGSDCRATRGQATEVVSLLDVAQRAMEGRIHTLEKFREEVQCAAALIRALRNGSAARPHQPGVRHIRPWPGAASRLQRFGGASGSNCVGYSRAVAAASMWEDAVVAACEAASRSLQLDKPENRRLMEIALACYMNGQYNGSVGTAAAAASPGTCRYSAKERAGLGLPLNAALTATGHADVSASLLPGLGSSEQGSLGTAPFGQQAGRPRDNSIAAFNYVALRGAGGDGPAAGGAVAGEAAPSDASRHGTAAAAEATLERLAAALVARVCTLLAHQGGLRRLLREGARGDVAGELYPAEEPSRIYSVCAQPCAADRSCKQEENNLLSSGEGAGLVSMGRGMRERETESECNVAALVSEQVRQVTVKILAICECACRNHSAGKEPEARGTGNAYARWVHSVIAQAFVNVVSVDMREGEAELLAAAADQLVAVIKAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI08</accession>
    <name>A0ABQ5SI08_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">S-acyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_013591</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF301">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="181"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="431" mass="47807" checksum="BD072D9CF6EF6349" modified="2025-10-08" version="1">MMINSYLKDQDSTDDDDEALWINPGQDNLPTNLPAVSPWRAPNAYNRNVRTKALMRAVRGTAIWGLIDIKVILWFVVHVSVPLLLLYIDEDARQIFSKALGRDQSAAGEDARGLSTEIPQDPGSGGVRWALLRPERLLLLVLLGLMATALGLFGSLYASNPGWIHAGFPAVDLALDSGRGPCPQCGEVPSLRSHHDKRTGQCVHKYDHHCWFLSATIGDLNHARFVWLLFVEVALLTWLQCWLMPLALSCGMMQSRWLSAGRRAQTGSPPSPLTSCPRVPSWDRMVVLWLLLAFGAVVLVLVMHLALIHGYLVATNQATLELLKGPRLPYLSRAYASLPPSRPGGGCKTGMTSDSHGEVSLTQIVRQHWQGVTPPRPFDEGLLRNLRIFFLEPKPHPYRYRTGITELALQPVATSTRRPQSAAWWPTSMTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIE6</accession>
    <name>A0ABQ5SIE6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_013831</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF6">
        <property type="entry name" value="AT11025P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019693">
        <property type="entry name" value="VAMP-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="9">
        <location>
            <begin position="5"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="173"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI69253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="257" mass="28185" checksum="32113AD534F9FD5B" modified="2025-10-08" version="1">MASPDVEVTPSDLRFRFQINKQLLATITINNPSSQRVAFKIKTTAPKKYVVRPSSGVVEPSTNVSIQVIMQAQKEYTAEFANCKDKFMVQTTVLEDAEQIEKDTFNKEVRKDLKEYRLRVTIEGPAAPPSPVPEANEADDEGVAVRVSAVPPAAPAPQLASSPAAEPAVDPRLRNAVDNLHAATVENQQLKAQVDRLQRERDELRRQLDIFQLQGGAFKTGASGPQQAATAPKPQLSMAIVLLVAVVAFLVGHYMRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIZ1</accession>
    <name>A0ABQ5SIZ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014658</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF3">
        <property type="entry name" value="FI21236P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="11"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="142"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="87"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="220" mass="24051" checksum="AA017EA91267208A" modified="2025-10-08" version="1">METQETIRLGKRCFVGNLAWKTSWQDLKDKFREAGNVVYTNVMRDDDGRSKGWGIVEFETPEEALHAIQNLNGAELGGRRILVREDREDRDIKQADGAGEAPRAPRPGRGAGRGAANGGRGGGRGRGSGGAVAEGTGESSGLQIVVQGIPWAYTWRELKDMFAEIGSVERADVVTGYDGRSRGYGTVKFTSKDAAEAAVTRYHESELEGRRLAVFIDRYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ17</accession>
    <name>A0ABQ5SJ17_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014705</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="10"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="455"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="497"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="672"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="774"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="838"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="782"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="796"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="872"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="898" mass="98911" checksum="CA415C2154530145" modified="2025-10-08" version="1">MAMEIIKVVEEAHESPVSSIAYNRVLKELYSSADGDKVIRVWDAKTGALIRTQGGHKGAVTCLAYSSTCKLLFSSSIDNTIGIWTDKGVLLQMASADIGPLFSLAWDSKRRYLAVGGNAVVLLFKVDLLEARKTTQQQRSVASGLKDTTVTLDPPQILKRLYPPFRGPEFCHTDVVSCMIITETGKIVTGGYDKCMCMYEYDKLDKPKEAFQRVKKCHTAAIVSMAYDTYTNSILTGSIDGSMKVWSMEGRLLDKFESINDQPVAVAYVPSTNMYWASGRFGRLVAYDPRAPANVTQYVQESSGLDRYNVEYMYAPMGTDMLLGASKQRQLIMWQYNHMGAYRTFRKHEDWVEGVIVVTPAKERPDDEDEETPPDEIFSCGADGKVLRWQLDAEQNCDVYVCMEEWQLHSKNIYCMVYSPSLNCLITGSEDNTIHLHYLHSVVPTYNDVPLPTDFTDHEARISGLALLRNNLLASASFDKTLRIWDLTTMKPVACVFNAHDSPLQCLEYSEERDELATCGMGNKVKIWSVRKPAQVKHVLSLDHADGPDANGEEDPSSSWTAKTNMNWLTKSELAPTATGIVLETIQQANRDVPEVTQVRWVQFRHCWVTAADDDTIRLWSPDGHKLQQFTYTGGSVQCIYVDNTNKLLVAAMLNRAAFVYDLDDPMPLARYAGHTDVIRGIGYVKDCDTYVTASWDKSLRLWFRPKDRKAAGGAAGAQGGEGGATPNMLLLEYDDDEEHFVSEYEKAHPLEMPKALTDINPMKLLQGMGVYDDDDDAPAGGRKKGRRGHKMVPEADLGLDPAAPDPPGSLGSKLDELGRSLLNEINVIAHKAAARAAAGVGPGGPGDLDERASTRSSQIPSGLHTRNGTTRPGGGTTGGTAGTTRLGTGTTGRGVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ95</accession>
    <name>A0ABQ5SJ95_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-phosphofructo-2-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF44">
        <property type="entry name" value="6-PHOSPHOFRUCTO 2-KINASE_FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="4">
        <location>
            <begin position="85"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="707"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="780"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="782"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="851"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="877" mass="94408" checksum="D5DDF10A951F6925" modified="2025-10-08" version="1">MYEIWRSRSSLGFQRPKLDGTQSPVFEASTPLGRIHSATVNFKDISIGPEALELAEGDIEEDDRHSEDSTNKSAILLKPPRGPHRTKYVIILVGLPGRGKTFLCNKIKCYLNWLGHHTNHFNVGSYRRLQKSDHEVQTAEFFDAHNPMGIEARNRALYAALDDLQGYLDSDIGQVAIFDATNTTVERRRLLISRFHGRFQYLFIESICTDEKVLEQNYRFKMQYSPDYQGMSMQQSLSDFKERIHKYEEVYEPINDRNLHYIKLIDMVTGRGHMDVNRISGYLPGKIVFFLMQVCKAGMAQARRIWLTRHGQSEYNLTHRIGGNSPLAPAGEVYARCLLEMLINRLPLNEEQKFSVPVSVWTSTLCRTIQTARHLPFTKLRWKALDEIQAGTCDGLTYEEIAQNFPEEFAARQKDKLRYRYPAGESYLDVIQRLEPVIIEMEREKECIIVVGHQAVLRAVLGYFMAKPLESIPTLDVPLHTLLELRPRPDGTMDFEYIHLSSEAAAVMEANMAAGPPPGPLSSGSFSSAQSPMLLSPIFSMGRNDPVAATAAMAVLTPLGGSAACSALDAASATAWSPSKISSSPFVADAAASDVMEVVTPSETVATAVIAAAGESDNTVVTSPTKSHHSRAPSLTAGMGGAVMSATGNNPSPSFHFAISPTDAVAATVVADGSGLESPSTRSAMLAAVVADSRARKVHVPFGATMGGGGSEGGAPPDSLLAGQLPLGSGGAGAGRPLSWSPATGAPPRGMLGSAPRAGSGETAASFFRDIQQVRRMLAVETDGEGEDKETASADDEGNEAERLASGVEKAARKLSAVPEEPALTLNHLLDRPEVQSPSDKGMPCVDEGGTKVGGDSGSVGGEGVNVGDGGDGASSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKG3</accession>
    <name>A0ABQ5SKG3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar ATPase assembly integral membrane protein VMA21 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014694</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019013">
        <property type="entry name" value="Vma21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31792">
        <property type="entry name" value="VACUOLAR ATPASE ASSEMBLY INTEGRAL MEMBRANE PROTEIN VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31792:SF3">
        <property type="entry name" value="VACUOLAR ATPASE ASSEMBLY INTEGRAL MEMBRANE PROTEIN VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09446">
        <property type="entry name" value="VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="11391" checksum="5B9C994189AB3ECC" modified="2025-10-08" version="1">MAPKAAKGAGVAQGPQYPAGAGRALFTVLIFSGLMVTCPFLLYFGSFWGYFDSLYALTIGIPAPENRAVASAIAAVLGVNLVVGAFIFVAFREVTDDSDTPQAKAKKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKK8</accession>
    <name>A0ABQ5SKK8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rhodopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="16425" checksum="021A22768A0B6B07" modified="2025-10-08" version="1">MSHPPPGYPGAPPPGYPPPGYGYPPPQPGYPPQPGYPPQPGYPPQPGYPPQPGYPPPAGYAPPHGAYPPAHGAYPPPHGAYPPPHGAYPPTGYPAPHGYPGGYPAHPPAVVVHGAVYGHPMKHKGYKYKGMKHGHYYGKHKKHKGFGKFGKFKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKN4</accession>
    <name>A0ABQ5SKN4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_014631</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058796">
        <property type="entry name" value="COPS2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25983">
        <property type="entry name" value="COPS2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="9">
        <location>
            <begin position="241"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI69979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="441" mass="51079" checksum="BCCA5F7AC3212643" modified="2025-10-08" version="1">MSDDEMEDYGFEYSDEEPEEEDVDIENQYYNAKGMLEGDDGKDAIDAFKQVVDMEQGKGEWGFKALKQLVKLQYKLRQYAQMMDSYRLMLTYAESAVTKNASEKKINSLLDFMAGASDMQVLQEFYEATLQALEKAKNERLWFKTQLKLANLWFKKQEYGRMNKIIRELHRSCQREDGSEDPKKGTQLLDIYALEIQMATEQRNNKRLKKLYQQALTVKSAIPHPRIMGIIRECGGKMHMHDHLWSEAATDFFEAFKAYDEAGVGRRVQCLKYLVLAYMLMESKVDPFDAQEAKPYKQDPEIVSMTNLVAAFQRNDINEFERILRTNKRTLMDDPFIRNYVEDLLKKIRTQVLLKVIQPYTRVRIPFIAQKLNIPAPDVEQLLVSLILDGRVSGNIDQVNQILEVGGRQDGARRYTALDKWAAQISSLHSAVQNKLVVNMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL02</accession>
    <name>A0ABQ5SL02_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11296">
        <property type="entry name" value="O-FucT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="706"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="812"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="718"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="876" mass="95766" checksum="0A70EFEA71B9CA2B" modified="2025-10-08" version="1">MLAVRLNRTAVIPVLIDNGLQRTGNNIFASANNQIPFSDMYDQPYFIQAMAAAGLRVMAPEEAPPITSYTTVSLVPYGWSATAPLAEQYSDKLHVAVDCPLLKMAASELTASDWEFMWAVLDALRPNPQAAAALDLALTRLVQRQRHSSREGRGEVRAEAATGSAEADAAASSSSSFNFLHLRIENDWVAHCQRWASIQDGMVRDNCFNYTEEIGSRLALFGLPNSTTLYVGSYWADVEESRRGKVFAQLAAAGYRVITSADLFPAGLGDSSGAGGDGALTRNERTQTETQSHGGEEARKEAGGLDPHRARGGREYKAMLDYFLAMRAERFIGNSVSTFTALEILERRRRGQWAAYYNGGNIPLASLMPGLHRLPWVFTYNSWSPDYEYMLRGAVRSALATRSFTPYCIFQGNDSSPIATWLQAQGVRLISHTPAWTEKLLEKARVRVKDNVHHSHLFKTPDMLVATFQRVDLPVVPVLDQYTYVLYTDADVYFRKPIRLDDFGLPLPHSVSMSYEFVDMFPYNAGVIVANLPTMRQNYAAFIDTMLANDNGLFYTNYGPADQGIMNKFYEADLRGRMLNQVFNAKPYNSFDPNAFIVHFHGPKPHDLLSFMTTGKCDFFSLCESAFLNGLCTYTKEWVQWVYDDLEALRLEDACAWLGTPALAQLLKQKWGIQEGQGTAGAPTAVSDAAAAVHVANRTEEVIQADGNDIEALSGKDTGNTAGSGARTGTGTSAPSASEAATSVSPNLAAGAATTSADSGGGSKTVQGQQQQPFPEDVQRLSQMRRALMQSVARQEKGEGAARREAVVGPPNHAQQLHQHRHQKAAGAEANDGPKDGGYKDGGQSLTDIDKFNGISADEAALEEAWQTLPARSAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLD1</accession>
    <name>A0ABQ5SLD1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">chloroplast protein-transporting ATPase</fullName>
            <ecNumber evidence="3">7.4.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_015744</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O + chloroplast-proteinSide 1 = ADP + phosphate + chloroplast-proteinSide 2.</text>
            <dbReference type="EC" id="7.4.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.4" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17928">
        <property type="entry name" value="DEXDc_SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3060.10">
        <property type="entry name" value="Helical scaffold and wing domains of SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1440.10">
        <property type="entry name" value="SecA, preprotein cross-linking domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01382">
        <property type="entry name" value="SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020937">
        <property type="entry name" value="SecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011130">
        <property type="entry name" value="SecA_preprotein_X-link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011116">
        <property type="entry name" value="SecA_Wing/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036266">
        <property type="entry name" value="SecA_Wing/Scaffold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036670">
        <property type="entry name" value="SecA_X-link_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612:SF11">
        <property type="entry name" value="PROTEIN TRANSLOCASE SUBUNIT SECA2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="SECA INNER MEMBRANE COMPONENT OF SEC PROTEIN SECRETION SYSTEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21090">
        <property type="entry name" value="P-loop_SecA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01043">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07516">
        <property type="entry name" value="SecA_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00906">
        <property type="entry name" value="SECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00958">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81886">
        <property type="entry name" value="Helical scaffold and wing domains of SecA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81767">
        <property type="entry name" value="Pre-protein crosslinking domain of SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01312">
        <property type="entry name" value="SECA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SecA family profile" evidence="14">
        <location>
            <begin position="171"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="257"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1535"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1551"/>
            <end position="1561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI70720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1629" mass="175406" checksum="9A64BD20369C254B" modified="2025-10-08" version="1">MKDTCLRSGLPGLRRAPSSIAPPGSSRSHETWNSPKPSTGAQQPRVADVSVRAGGMDARPMVLAPPPSQRPPPLRLLQQQLPQHNPHHLLQSLPHSIPAGAQAVPYSFTCRGGSVVFLGPLHHPLARPTTRRVAVGALSEAAAATGVSSGELQQQQQQQPPKPEYNLPAHWQEQMARAPLGRKRVLQRYYADVIAINNLEPEMRALSNAQLRAKTGEFKRRLAGGQSLQSLRVEAFAVVREASRRVLGMRHYDCQLVGGMVLAEGRVAEMQTGEGKTLVATLPGYLGALTGRGVHVVTVNDYLAARDAAWMGKLYRFLGLTCAAVQSTSSVVAARAAFSADVTYVTGQELGFSFLRDNTALSPQDLTLRDDRFHFAIVDEVDSILIDESRNPMIISGKGSSDTRVVQLVDKAVRRLWSQVQSDIAAEVAAHGPPGSLSDADLQRLEKGLKSRYYTVDEKSRTLSYTSTGTHLIFLYLLDEGAQFADSYPGVHSLWEEDVPWGRLAVTALTAYELYINGKDYIVRDGEAVIVDPSTGRIRPQTRWQGGIHQAVEAKEGLRVQAENLVTATITFQLFFRQYEWLSGMTGTAQPAAAELFELYGIKVVPVPTNRPSRRVDHPPRLFYDKAVKMHCLAAEVMQAAEAQRPVLIGTTSVQESELVLNYLMQTVYPAITAATTRRAAAAARSASTAGSFASALSAAVAAAGPKPRITLLNAKPELVRLEAQVIAQAGLPGAVTIATNMAGRGTDIILGGNPEGLTKLALMRLVYRRLLKQEERETLPMLPLSQLDPYDTDDVGALSRISYSTPEAPHAGLPRPLHEVLSTAILISFAAKHQTEEGEPALFASGGGGSGGASLSFGGSMTGSYGGSLDELGGSGGAAVAATGISYGETAELVGWVMDRAEVLRRKVRGALRRTYRDHAGGLERLNYTLCVAPVIESVLAERERELYDKQLAALSAAAAAAGVVRGAPAGPGSSFTDDDEATAAAAAVATAAAASGGEDDGEEPMAQVWALRAALHLWLWFDQECARYRDEVRTAGGLLVIGTSLNESPRIELQLRGRAGRQGDPGETKMLLDCVDPLMVMFGMDKVSAMLTQLGSQLQTGPDYLEGGPVDYFVNYVVRWQESMFQNMRLETKKYDAVMEEYRHNLYTLRRLVLCGGDMQRTLIVYVLIQRYVDELVGRYLDPGQAPARWLRESVRLDSLPGAPETQPDQLPVMVSPLQAVLICLRTLVNPLGRTTKTLVDFSEVTEAAAANPTVRKMLELFGVANPRADPRDAAADGVDTQYIPVYMQVQELEVLAPEQLRNLAEYIAGTGPLEWPVPAAVEIRGNFKLQLQLRTHRKLFGYGAVRSAGIMPSEVSAAAAAAQAAERLQLSAAGGLIQRPRLRGPFAEKVAVLRNYLGELLIGCYEARRLAARLTFTASPSLGFTDIDAEQQVCAFEQQTMLLWIDALWSCFLEDTARLRNAVNIRSTNGSNPVEEFRMEANAAFLALLDNYRDAVLDKLLVPDFSIYAPLESEIADTDLVGRGGLVTQVPTEEQPAAAAADVSGRSNGDGNGNGSGNGNDMFENVHDNHLSFVVSGEAVDKPNHNGHGNGAGAAAAAAVTADYQRGGSTSTNCGVPVGSAVEGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLG1</accession>
    <name>A0ABQ5SLG1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyl hydrolase family 32 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35279">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35279:SF1">
        <property type="entry name" value="ARABINANASE_LEVANSUCRASE_INVERTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="367"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="46912" checksum="6C2CA5F830D4B731" modified="2025-10-08" version="1" fragment="single">PRLRSLAEGAASHPSSQPSELQPAGLVFGQGAPGSWDEAAVGAPVVRCYVGDNEQRWFMWYSGRRSDSPAAAAGVDALVPSSGSIGVAVSRDGIHWVRGYDAVEGARGEDAAADVGTVLSPNQDWWTFDTCHLIPGDVQVLSNSSVSSGVGVYWMFYTGGDFEPVVLPEGFPGSGGRSSSGERQAVEGLRLRPGLAMSQDGRNWARIEADHHTGALFDVGAAIEKTEGLIFNGGMQVLAVGPRDIRMYYSAYDTSRRRFVVALATSPDGFKWIQKGVVFDPAAFAGAGAASDDEDDGGGAFDALGPAALSIVRDIDNRQFLMFYEAVATDNRRSIGLAVSKDGSSWRRYPVPVLEPEVLAGPGAGSSGPSDPWDSGDVGSPCGVSMSAGRWRLYYGGRQHSGKGPWQGIGLALSVEGGATFEGVPVQYKRRVPRPESAPQPVASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMC9</accession>
    <name>A0ABQ5SMC9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="668"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="797" mass="82496" checksum="40D788A12A063472" modified="2025-10-08" version="1">MKTLLVGTAASGASGPAAIVAKVPAAAVAVNGNRHVRDVIRAATSSPPTGGLGQSESRVQRSADGGIAPDGDGPGTRTARRHRGQGRGSGTGSVTRNGDGGGGGRAQGDGADAVAAGMEALSAAESNSGNSCTVSTGSRGSRRGGGSAGRGKPASAPSGMSLSSRVRACRSVEELVGLYDTAQREAEAAARSRASSAGAAAAAAGLAAGAAGLAAGTVWMFLAAAGSLVAGWRKRIPSGYRTTRLGEAKEMRTQLSRLLAEACGVVLWAERLGPAKVCLLLSLMVVAGGRYPGVEQHLGLLVEDSLNSLEEYSSGEVALAASSFARLHYFPGTGWLETLLEATSGRLSSWRPIDLGFLMWALAKWRTKLPATWLQEYYTAFEGCGERVRERELAMVCWAVGVGAVGGAPRPPDSFTNRLMMEVQVRLPSFGSQGLAHIAWGLCCCDVKPPRFWLRDFTMAAKATLPYANNGMSFSNLLWALANWQGELHPGEELLAACCKHSARWLCTLDSRGLAVTLLALSDLGHIPPVEWVGLALETAHRKLTTTDPSSAVAILWAISRFERQRAATAAAAAAEAAAMAATGANAAAAAAVPLPPRRRRLTSLTPEELLLVADDIGFGFGLDADGRTLGSAGTPLALRPPRKWLVRYYPLIKDFAMAAAGVVPTAASRRSSMKTSTPLTASLGRGASIPDSTEVATDAVATPSRFAFAGLDTNQMLDLAVALADLYVYPGDTWMAAHEAHMALTGGGGGGSGRNGSGSGSRGSLTPYQLKLLMASYAALDDTAEEYAAPRAGEMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMI1</accession>
    <name>A0ABQ5SMI1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycerophosphodiester phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="422"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="648" mass="69515" checksum="4118D40D5BD04EC6" modified="2025-10-08" version="1">MPVHGTFASIRTWLDHSFSVLIKFWRELSARTRPHSLLGAQARWQHSPPRHQQGRLQQMALGGVLAMFVLALLYFDSGSSRRRASTAVVLPKSSLTSEGQVPLNIYPKNSAADDKALCRALSQMQVCSHRSSLGQSDKVDEAPLLERMARLAASGIVCYDIDVTATADGQLVVGHPSHIVTELRSAGRMVAPGQLDTMPWSEYVSAGLDKRHPLLQEVLAVFTKIVKNSDLGQDVGYSGGVGRETAGAGNDAASEVVGATAAGTTERGEADGGVGGGEVGSEAAQSAEAVALTDILHGGIEAVAQGVQQEEEQLRQQEGEEGVTVVPTQGQEQKDGLDKHSEQQKQKQRRQGRRPPALGAQMQQQQQQERTKSTDVVEEHQQQQKRDQQQRRQQVGQGQGRRRLHVLQEHGGDKAAARQQHQQKGADAQGQEQEQGQGQGITAGWDHPTALLLVELKDSALRVSVAEAVHNATQVLGIQSRVGLWLPRVGRVEGPSGEQSGGQGDPIQTQTQAVLERLQQLGSGLLKVLGLPDISRLTNGTRLPLPLMLQHGDATTYDMFGPSIMHSDAALAEVITAAEGRPVVTWVVDTIDEAMRVARAGGGLIVANEPLLMRSRLHAASTTCSGAVDGEAARAEKGKKAWAVERGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMP2</accession>
    <name>A0ABQ5SMP2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015927</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="129" mass="14119" checksum="C2E34CE2A935F46E" modified="2025-10-08" version="1">MDLTEECGTSVVIRGWYTRHHGLKWGGVGVETPPRGLLSQKPGCGANDRTRPRRGTGAFVVRPVKAGSGREAQSRFPDRPWIDVLSKGDMLQDTLSAANTLMRQLMAAAQGALVAAALDTQLWQQQMRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN68</accession>
    <name>A0ABQ5SN68_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OCRE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015992</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16166">
        <property type="entry name" value="OCRE_SUA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039905">
        <property type="entry name" value="CD2BP2/Lin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041591">
        <property type="entry name" value="OCRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035623">
        <property type="entry name" value="SUA-like_OCRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138:SF3">
        <property type="entry name" value="CD2 ANTIGEN CYTOPLASMIC TAIL-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138">
        <property type="entry name" value="PROTEIN LIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17780">
        <property type="entry name" value="OCRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OCRE" evidence="2">
        <location>
            <begin position="512"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="563" mass="60059" checksum="1102450582A88104" modified="2025-10-08" version="1">MKFPSIEIDKRDRDEHTDTLIKSLLDEEKASKKQRRGSPQADVGAKSAVPARKGKTFAEIEAEEGGPIKESLVGMAHTKRALDRKKRDLEVGDTDVVTNTERYEEDTLEVEQEDGITFEPFNLKQEREEGYFDEDGNYVLRKQGGEEGDDEKDAWLNSEEAKVVSDEVRRRIENQRARAEEAARRGPLTERQIAQRKADMAAMMRPGETITRALKRLGGSGGAAAAGLTHRAMGKRERARLLAQQQQQQQQGADDAGGSSAAKDAKAAAARQQFVHMTELADELLEEGELEIYDATQEDLLRSARIWLPQLAGAPLPPPSSSMSLLAGGGTSASAATKHANDTGDEDEDMFADDAEDGGDGGRKDMAPGKGAAAAAAVAAVDQDQDMFSDGDEGEDTNCGGGRREDAPAATDSGNRDGVTEPGEPGGQAIATAASSVAAAAAQSSEGDTVDYASWPIKELKRILQERGVDPSGFVEKGDLVSKIKEVVDGVGPAGAGAGGIGSGNVSAFVAPAGYVFDPSSGYFYSEESGMYYDINTGGYCQAASGQWFYLDPLSGTFLEWKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNB1</accession>
    <name>A0ABQ5SNB1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-dihydrouridine(47) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="3">1.3.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_016639</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5,6-dihydrouridine(47) in tRNA + NAD(+) = uridine(47) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53364"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:53366"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(47) in tRNA + NADP(+) = uridine(47) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53360"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53362"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Dus family. Dus3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.89" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="14">
        <location>
            <begin position="85"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="447"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="476"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI71425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="488" mass="53504" checksum="BE50A1F7FC01D07B" modified="2025-10-08" version="1" fragment="single">LANLRSPSRVVCLPQQPPFPTAAARQGWSFTRRSGIDHVLPEDERIVDASGPSSSGQAEFDYETPGWVRTRNATFVEQLRGKLLLAPLTKGGNVPFRRLCLHFGCEVTMSEMSFTKPLLKGDRVERARLYKASGESMFGFQVACKTISDGVAASKLAKEAGAQFLDINCGCPIHEATRRGMGSAMLRKPRALAKMVAGIAAESELPVTVKIRLGENDNKINVDAVVGFLERAGAAAVTVHGRTAEQRYKKAADWDRVSDVARSHSVPIIGNGDILTHYEAHARLAESGCAAVMAGRGALIKPWIFKEFKEGREWLLDTQERVGVFRLLVSYMKEHFGDDERGRRKAWYFFPWHFDFLTRYRPLPEAVYGAQSRERPLILTRQDLTDARVGETPEGLSWAERLLRCEHPDAHGAMADALWEAGSDAEALAALERIGAERVVCWEDDVRSGGRDGDASRGGGLGVGSDGGKAGIQGKGSRDRAGEELERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNL2</accession>
    <name>A0ABQ5SNL2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="540"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="689" mass="71537" checksum="E617AFBD5746063B" modified="2025-10-08" version="1">MSQLKKSIGAAKACFTTVRTLSTALQALESVSIYLDHDLPLESLSYSTSAPERRLALSPSAVGWAITCYHTRDSLSKKPIPGLSTNIAAIRRQFPIDIVNRGRPSFSGWELSIQCLAQRSKHTRAGQTHAGSKRSSGAGEQTVSGTSTEQQRPRRRLPRFKPSRKIMNTASLRELGELTAAEVAGWNAKELAAAARRAVSLWRHEQPGAAARVQRMQRLPLLPPLVLTELSHAAVVNGGDSSTLSSSTHSTTSTSTTGCGGGGGGGGSYIDGRNEKGPVLGSDGGCSGGTAELPSKIALRAAPMPAAPKPPPRELTESAPATVATELSAARWTLAGAEADVLSAAAPGTAASPVFTVQRSLLPRRRLTGGVYGASVVERPLRPHEHRLAQLVERLVQAANGRLAEGFGSRELSSFVWSLVTLGYWQPPLVPLVAAFADYGAERLRAADPIAMAIVLQVLAKARAAATAGQTTATKMLELVPATAAAAATSLPRCLPSCEPRTLAGLLHSCTVLLSPSPNSSRSSLRGAIEVSEIPFLEGSTESDATPATKSVSRHESGSESRQNGSEVHLDGRTTEALAAVAREVTKECVRRRFIGFGPVSLVTAAGALTALCRSPNPGLSPNPGLGGGDDGSAVWALLAAAAVSPTAPQMAGASPQQWTMLLRAARAQGGPSKQMLLAGAAAAGVSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNT3</accession>
    <name>A0ABQ5SNT3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046380282" description="Pherophorin domain-containing protein" evidence="1">
        <location>
            <begin position="39"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="18652" checksum="E9C230DF600D949F" modified="2025-10-08" version="1" fragment="single">MLGLWFRSCQSYLPVGFRRIFKLLLLIWVSAPSPGVLAVKRPAPPPPSPPKINMTLQGKLLYRTNGNWTLMVTLSNTYMLPSQPVIAAASGSARAVPTGSWISLVCLLQSSNSRSCSSISGVKIDKVAAPAPTTNVTLKVMVMVLSLTASTECGSRGGANVSEVQNAFVGPSGYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNW0</accession>
    <name>A0ABQ5SNW0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_016465</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="6">
        <location>
            <begin position="470"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="697"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="704"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="742"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1352" mass="149344" checksum="BFAC28534B159673" modified="2025-10-08" version="1">MGKDRLDEPLDLHNYSTWKLRFQLLCAELGYSRALLQAPIAEADVIISDKVKSVMAKNVKNHHLQTIVRAANAKAAWDALAATFASTCNTRKVALRQELSDFKMANGEELPIYISRARQLQSDLLDVGHTVADTELSTIVIKGLPSKYHVVTTALTMREGELDFTQVVARLMAYVIPDEDNNESSSAFITHRSGGKFNPTSRHQTSKQRSKSNAICHYCGKPGHFIGVCKKRLADQQQQNNGRGGGNLQRREPTKMAYAVGSESRSDAWHLDSASEWHITYDASELKDLRAVRPEEKFTLVGYDGSRHQPTHVGQYTMWSNTVKGLQIELKNVYVVPKATVKLISAGIFDERGATILLGNDKALVQAKGRTILRADKVGRLYAIRHHAAGRSVGKGRSCAAVGSEGSKAALWHRRLGHLGYKSLERMCREHMVTGMDLDRASLEAARSRICEPCIYAKQTRGPFPSTGHKAVKPLGLIHLDVCGPMPETSIGGSRYVTTLLDDCTGFSTVAFTDTKDAVRKKVKIMIEALENMTGRRVKEARTDRGTEFLNTVLGAYFGEKGIIHGTTVGYSPEQNGAAERLNRTLLEKTRAMLTESGLPQKLWAEAMATANLLRNVSPVVGASVTPHEAFMGVKPDISHLRVFGSTAYAHVPKEKRNKLQPVSKKGVLVGYEQGRQYRILINNLISTHSTVKFDESTVGDRYEDSDEEDVEESGGVPLPGGGGDTPVNTDMDQGAAEVNHGASTSSGQTAAPQRHAGIPAAQFPRMQNTAQSRGGNGGDAMTGEGPVQQQNHNNDGADVNMQERRYPLRERKRLIEWRHEPNGRVTFGRINAAIEEEVPEPASYQEATDGPYAEEWRSAMDEEISAQLTNGTWELAKPPPGARILPCRWVYKVKRAEDGEIERYKARLVAKGYEQRAGIDYGELFAPTSRFASLRMLLAVAAAKAMPIHQLDVSTAFLNGELEEELWMQQPPGYESADPEQACRLLRSIYGLKQAPRCWYMKLAAELDKLGFKPSVADPALFIKKDEKEIVYLLVHVDDIIITSEDEAMIRRVKTEIGQAFKIRDLGEAKVFLGMEISRGAHGEVKLSQRRYIEELLQRHQLVNAKPRKTPLPPGTRALPAEDNNKELVDNTKYRALVGELNYLATSTRPDISQAMSVLARFMGNPSKVHMGLALGVLRYLAGTKELGLCYDGDGDLTMVGYSDSDWAGDPATRRSTTGYVFLLNGAAISWNSQLQRTAAASTVEAEYQATAAAVREALWLRKLTRDLGLRTQAIQIRTDSEGSMSLGNNPIMSVRSKHIDVQHHLVRERVLRKEVALSHCSTEDMVADVLTKALGEIKFKKCITAMGLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNW2</accession>
    <name>A0ABQ5SNW2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hexosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016342</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047322170" description="Hexosyltransferase" evidence="2">
        <location>
            <begin position="29"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="612" mass="68543" checksum="AF75AE432F7B7855" modified="2025-10-08" version="1">MRSMQRSMRPLIAVLLCLTTGLVHTGSAQKSRSNDHLILKGIGLLPHGVASEEETSIDTDGAPHIEKVFIHRFSHILENRTERSKFEKALQFILDAEEHLSSTFNAAPRTAQLLRDHVSGSAAALRAIQLARKLSSQITSSEGAGFQAKAATETLQQVAIQLKDLSHASCHPITRRIILATLVRLQLLLHQSPLSDEAKVLKDSPDTPRLLFHICRLLFNLHRQGGAAKGAIEFLHISKSGGTSMCSVADRNGCTAESVTNYGNCMVRRFDDRPRWVSAEVHNQSAPLDGWRWFYRYSVPRGNRSCEYRDEYMQRMRFTLYSNEFAVHGGLEGRNSDGDDGRTNGGDGRASGASWHSTHLCPQFLNVVMLRQPLERLLSHIKWIIKVYRAEYGRRSEAFFRARDAEYWRAFAPAAVDNYYIRLLLGEGVFYLPSGTVNTTHLEAAKLVLLQYDVVLLLEAGDIDELWLRMALGWRVGLSSAHARVASSSRTTNELIPFDVDDLMAANTEDVQLYDFGAVVHQLDGFMYAVMLAAGIQPYSPYNYLEASDPGVGKRVRCGYVTRKQQLLANVGVNISELHRAYDSEFPRILSMDERVRAPPPTPTPPVQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP53</accession>
    <name>A0ABQ5SP53_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="99" mass="10974" checksum="E08318077539C23D" modified="2025-10-08" version="1">MDHSVSCSETRIIQIAEGEIAEGEIVERMLPRLPDWAHARFIQWEAGFFALYFPGGPIPLALLTGSRFSPILAHEFNLKLPPPEDAGHPKDDSQPEGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPF3</accession>
    <name>A0ABQ5SPF3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016778</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF39">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="4">
        <location>
            <begin position="495"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="293"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="411"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="103"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="767" mass="81175" checksum="C0663196903BB951" modified="2025-10-08" version="1" fragment="single">LEQGVSHAAAKHSTSMAVQLGEALVARGQPGPPATASVGQLCSLLRNSLGPRCGFLCLGCPRNLETEMSKTQLQELQSTSNKAFHALKLLGRVRAVLLSTQVDGDEDDEDGNEDGTEADAGGCAGRRSTVLTRTAGKGSTSGGRRRGNKGRCKRRRVGDPCNAWAKGVGYGGGGGIGGSDELAAVKAAQERQREADDEVRSCLKTLLEVISPSRYPGGGDGGGGRALASPPPLLHAALQYGGLPYVLRLLFQNDSLLDMGQREGMYREAMALVKCFTNSMSLLELLVVPASDPRDTSGPGAVTPPCGATTSDGRGAGGSGAGSDPPLSTIFESLGRRCAIFKRSAQELADGVEEDIAALGLALELGALAEEVSTSASMWRSSIGKAHGALHAFLPDGIEDGDAAVLACSRGAGSRRDGRNVPLAKHGRRGKRPAGGAGPSSAAGDVLSTHAAQRSEYVSVMRPLQFQEVSLLDDHYFRRDAESSRSSAGGDVMRRRLKRITGEISGLATGLPLDWEASAFVAVDDNRVDVLRALVIPASDTPYANGAFVFDIYLPPEYPNVPPKVQFLTTGGGRVRFNPNLYQDGKVCLSLLGTWTGPSWDPAMSTILQVLVSIQSMILVSDPYYNEPGMESEASTAAGRQGADDYANQQRYNTLAFSILPALEMVTQPESKSCSLPGGSTFRDVLTQHYRLKRAEVLRQCDEWMAEITASASASASASASSKAIAFHEMYQRNKEHLRNYTEAILPKIKSLLQSLCGEHCSTDGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPP5</accession>
    <name>A0ABQ5SPP5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_016012</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAF13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07978">
        <property type="entry name" value="HFD_TAF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF5">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="234"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="241"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI71091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="273" mass="28156" checksum="58305B5ACCC1821C" modified="2025-10-08" version="1">MENPAVLGTPPSASKGRGRKAGAGRGAGMGRGASAIKDISPVKPIVASGTAPTSAAVPAPAAPVSTAPGGGVTGTNAAATAVAAGLGPAGGIVKKRGRVPKAKPASDFPAAGGDSGPEDAPDASMPYRGTFTKDLSRLMYGFGDYENPIQETINVVEDILVEYVREMCCAALNEAARNGKLDRDRSSGTSKLKVDEKDILFLVRKDPKKYTRIRELLDMQLLIKEARKTLDVEKISEGDIDTGTTGAPGAPGAAEEDPGEELPDIPDEDIEMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPX9</accession>
    <name>A0ABQ5SPX9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="136" mass="14967" checksum="640BDC3FC33D09B2" modified="2025-10-08" version="1">MDMDVSLNLEAINATLYSDDGEISPASYHEEQTPLLQNLRRSSGVDIATQTFFTFEKATQTPSRRTRDGQPILPIAERSPSTTHSAAELPRDVVGYDAMEASDAEPPAKRVRCVSTAGCRCRRRHRSFLSVPSPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ90</accession>
    <name>A0ABQ5SQ90_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_017196</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000125">
        <property type="protein sequence ID" value="GLI71829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF8">
        <property type="entry name" value="SD21168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="6">
        <location>
            <begin position="32"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="11817" checksum="16728087C55E2DCD" modified="2025-10-08" version="1" fragment="single">TSLNLKGKVKRFLNVMLCLGIMTVSASVARRPNFIVILVDDLDETQDSSGELAMPATKRLLRDRGVTFTNHFVTTPICCPSRVSLLRGQLGHNTNFTDVLGPHDLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMI3</accession>
    <name>A0ABQ5RMI3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4079 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000544</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025067">
        <property type="entry name" value="DUF4079"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34679">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34679:SF2">
        <property type="entry name" value="OS02G0122500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13301">
        <property type="entry name" value="DUF4079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="15829" checksum="5436D8FA74695912" modified="2025-10-08" version="1" fragment="single">GEEIRDLKKGLPAAGPDGERPPSPTDTLIAQKETERKELLKGEYRDKHWWWGSLLLASGVGIAIEGCVNTYMRTGRLFPGPHLYAGAAIVGLWAVAAALVPAMQKGDQNARNAHIALNAANIALFAWQLPTGLEIVGKVFQFTSWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMP3</accession>
    <name>A0ABQ5RMP3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA-binding S4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000611</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017506">
        <property type="entry name" value="PSII_S4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040591">
        <property type="entry name" value="RqcP2_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03069">
        <property type="entry name" value="PS_II_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13633">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION RESCUE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13633:SF3">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION RESCUE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17774">
        <property type="entry name" value="YlmH_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="2">
        <location>
            <begin position="173"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="248" mass="27255" checksum="BE42E2308AB8BA4C" modified="2025-10-08" version="1">MGYRVDRLPATPHRCRLYGRTDRPHGRRVLAMGGIRSGRALQVGAGPGGAHRAAPSRPPTARWRRSLAGLQDLRTAEVKQSKVKGNFMFDPAKHPDFLGAIIGTGVVREKIGDILVQGESGAQILVDPELVEHFEMSLVQVRTVPVEVHRIPLSELSVRPPRQEEISSVEASLRLDAIASAGFRVSRTKMGEVIKAGDVRLNWRSVSKTSVEVKAGDVISCSGKGRLEVRAVEVTRKEKYLVTLLRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNJ2</accession>
    <name>A0ABQ5RNJ2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF80">
        <property type="entry name" value="GRIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="880"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1827"/>
            <end position="1879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2486"/>
            <end position="2507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2519"/>
            <end position="2547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2663"/>
            <end position="2725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2957"/>
            <end position="2983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3365"/>
            <end position="3386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="178"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="502"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="931"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1129"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1489"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1884"/>
            <end position="2027"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2064"/>
            <end position="2126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2155"/>
            <end position="2291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2320"/>
            <end position="2354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2559"/>
            <end position="2625"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2810"/>
            <end position="2900"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3028"/>
            <end position="3286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1846"/>
            <end position="1858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2684"/>
            <end position="2706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3386" mass="361754" checksum="92040BF701C48706" modified="2025-10-08" version="1">MAGMPSSLSVGAMPAARSGILPREPSFGGFGTGPSPAAVASTSLHAQGGLRQAGGALGPEASVASAPGQVYPPPPPAPLHTNPLRHSTPGLHGPPGFVSALRADAFLGSAGAGGDISSALLRQQAMVTPGPVGGGGGAAANTMAGLVGLNRGGVGMGMGITGYVHSMQHAQAAFTVERTALEARCRAAEEALTQSERRFSVEVERLQGRVSTLESGLAAERQLCGQLQEQLQEELKRLRVATEAETAARAAELAAKEMLAASNHSLELERRRAAEAAQQSTQQAGELSSRVAALEAERAGLEKQLLQEKDHASTLHKQLRDAQRQEQALMEKLGASEGARQQQEMRLEAAAEQVAAMRNELQLLNAKLAARDKQLLKHVQEIEETQLAALRSELAAVQSRLQRQEGLYAALRASVAAIHQALGPQALAAAFGYSESLLSGFTAGASEYGAVADRLLSQREVAEEDAAAVVLRVRELVTQTRNLYMASEKREVALTSETDQAMSALRQTNAEAEARLASCNTALRTATQRVEELTGNLQDSRNRCSELEGSLQAAQHARQAERRRCEELQTAVGEMQQEMAALRTRAADAEGAQRELSASREQALQLQHQLQELTVLAQRSTDAAEIKEERIRELRAALAAAMDTTTAAEGQTGLLIHEKQQALEALDLERRRSANLEAVAQSTEQQLDKIRSRADAVEASYRELCQQVVVAAKEAAAHARELQQQQQAGGVVVPPVLSGASPRMAASQLAKAADDSAAQLTALEGALERLARGSSMSAAGVTPTPVRGSWAESPLGSGFVQLNDQAALPQVVMRLLLGHIQQLASQQALAVQYLSTAQQHLHNAVHVGEGDGLPPLSSASKGAVAAAVAAHRAAGVAGTAHDADGSAAGTPAPAMQTPPLAPLPWSATLAVPLHELARKVAAASEEVLEAVTDISRREAEAEQRVDEITRELSNARQEAAQVAVLQGRLAACEVELEHVKATAQAEFGRVQGEVTRAFVEADKLREAALAEAEDRVRAAEKAAADAAAQSQAQAVAAEALAEARVRDAEAARSAAVALVTSLQAELAQVRSLLDSSRAEVHGMQEEQAAGLRHVASLNDHIRRLGEEVTRWRKAANPRTRDRLVLLAKVRVLRRKRTQLQESLAAEKHRANQLSSAADARRERIRRGLIQMRQLQIDNVQLSSRLQEAEARAARADSLVSRLEERRISVDQDRKSQTEKAAKLATQLADANKALESARRAEAATRKDMDRIMREQLEPQQRRTAAVLEELERAAEALQESQANATRLLQQGEVLRAELVDVKRGAEADSQQAQQRLAALKKEVTERDATIASLRQQVAAAQASAATAAALLPAISSGQLGDAAAVQSHLIGQLGQLQAANAELEARRTAVETELEVLRVRLSRSEAAGTAVALTPGAAVSGTSPATTVSLPSSNRTLVDLIVPGHAALTPHGATVLASSAKIDDGSLSAKAFSARDQDSVITGVKDQRIHELRAALTSVMSERSALQRELAAAREEGSAEFEAQAAHVRMLEDEVHRLAASLETAEKQLEEVTESARQTMLATEAKAAERVEVVQSQAREHLADMERRLREEIAGLEKRARDGERVAAEANARGEERLRQAERAREQLAQMQALHASAEEAVRQLSSQLRELQQGNSSNAQAAVAEVQQLQATADSLRLEVALAKDAAETYEAEIRRLTTQNEVAASQCRTMASQLQNLAEDLATARTRISTLQQELVQQKDECETLRRANDALQISLAAAEERHVDSEAQLLEAEAALSRQQGLVRQVQVENERLHEQFGELQQQLQQLTSERRAAVAAASAAAAAAARSPSKGGSPGFVSSSRGDTRSEVGSERGNHNINTSGANGPPGRREAPSPLGGLEARLLEAAVTDLQAKLDALQIELVGVEARAKEAEADAELLRTDLDGAKARQRAAEAKAGELQSRLEASLLGSAQGVSALKEQVLELELHLDEQRDREQAAQAALMRATERCDELQVALAEAERRAEELQHRCEDKELEVGVLQAQLASASAQRLAQQVEAQQLGVTHGSSGQTITSALELELHEKTHLVDALQDDIQAANARAEEAERELSELRSFVEAMRGEAQELERQRQSAALEAQLTAEANARLRADSSKLADRLSKVQNDLLEAQDGRDKTSSLLRAAEARLAEERSEGESLRRQLEAAASQGRGADLLWVQRVADLEEALSAARKQAAEAEDAVVEAAAAQVRSAGLETRAQDLEAALAEAETRAAAAADAEARIAGALAGQREAHRQEVTTLQLQVQGLQGRLAVCERQVADRDARLEGVGTQLHALRGHTEAFELQLKEVRLELRSALQENEALQQKIASMDQTVHNVEGGASVSGLSLSTSSHAPQQQLVGMLTENRDLLEKLSSTHSKLKQARAKLAQWEQELRSKDLEVERLRSQVAAAGGMVATANAADSIIEVLRSELQAYKAALAASQEEAAASAAAAAEAQARCEALSQRLQQDGERRASHSQASARAVEYASPSMLERLHQQQHQYQYQGGGSGSAAAPASAADTDAEDTQNTLHALMHKVRSQRSEIDTLSTELQAANAKRTELAKEAATLDQRLAEAEIRERDLAATAETLRQQLTLTQQQLENRQMMLQQLTSISYQGQKGQGQQQGHKNILGLLGYPSSSSGSPQVMWGTAGDGEGPEDGPNSASTSPRSQATRPYRSTITLSPSRGGAHPSASGGGGAASASSSYSQHQLLLAQQHQQQATKLERVVAMWRDACRAKDARVQELQADLATFSSQLERARGDASVLTAKVLERDQTLTSCQHHLELTRDSLGAQLQAAEEKLEAKAQRLAAVEDELQNREDELRDVRAQLQAAERSLMLVQAESGQQREVMAKTLADLAAATRQVAALQAEIQDLQQRNAVSRSGTQQMVEQVHHLQDQVNRTETMVGRLLFAARTALSMASTTTTARTAAAAAAAGGGAGADGTTARADSDGRSDAGGGRGGLGSVLSLASSGGSLGGSGGGGGGGGATALGGLPSSLRLLEELILALSEELQRRNAALVAAEAEATGLQGRLAAREEDAKGLETRLKSAQDSLTARLEEVSRLQTALRRKEADCEAVETEISAQIADYQRLKQRLLQQERDSSRLSSDQEALLRRSDELEATLAAKQEECALLMKKCAQLEEQLQAGRSAMTALQERVRTLDAHSEALQRNNQTAALARQDTAAALDAARADLRAASQQAAAYREQLEMRNNEVRELNSMLQAWEAMRLGKDAQIAALLERCKRHEEDAAEKARTIEALRRKLQMPGRMSLSGIAAPAGAGAAASTTGIGMTPTSSSTTTTPISMAYQQQQMQQQQQQPSLRASSGNMALGARHIHVHMHSPAVVTSPPNSGMSVTGPHAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNW6</accession>
    <name>A0ABQ5RNW6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="296"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="409"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="530"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="761"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="919"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="991"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1237"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1290"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1392"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1446"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1551"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1591"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1704"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1761"/>
            <end position="1817"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="209"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="318"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="637"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="761"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="936"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1008"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1392"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1719"/>
            <end position="1729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1730"/>
            <end position="1740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1817" mass="184437" checksum="93393A1A119F746C" modified="2025-10-08" version="1">MKESEMPTLEPANLSAKGPGQEIMDEISSAQQYVLDLQVLFYSLKTVTANAQKEAHDMAENVALASAGAAANGPLTGYVGEPSSTGQVHGSHGGNGTGSAVVTAALQKHIEDLTQQLESERKERAKLQEQYDQAVVMLRKSYGQIVDDYLFATEQLHVRTKQLEGLMETAAAPPTSDQQQTRVHLPPANYVHRPTETLPQPQRDHNRDQQQQQLRNRYQGQVPVCEEPSESWDAYSPDPATDPAGYGEGLIANLVDTAAAALAAAAAAVSAAAPHPTSFPPEYPQSALATDRLGRRLTGAGSSGPLATSAPGGGVRTSSSSQSCSRGSNASDGEFADVATAAERAVVAGPPEQLLAEEQAFLATQRSGKHADDDGAVVLDNVCSGLMLIPGSQSTRPKAQILRTKVQTQLPHDEGPGPAETPPPALSSPHGTSQGPTPREIHGGTSLVAAQQLPQRTEVAAAAVAAVPQSQVVDDAGAEASRRSSTLHWEAAESLLAFAMKRTLPEHVAAGAKYSCAPVDVETNVLGSGLESAEPADGAVTPSTGRPDLTSARSASGVGPAPYVGPRPEPSARLGTATVDGVNGAHATPSFETYRAGEYHQPRVQDGLQKGGGGASSLRNSELHQKQGRRHHYPNCDTDREREQGREQSARQRSGGGASDAGLAAVGVWAATANSAEKRPEAGGARVFGRPRLLSEETNLTPVAVDRVADQPDEAIWQSPVLGSSASRNAQAAALAAASELRSAAASASAERGRWDSVAADWERRGDHREGGRKKDGRGEDRPISSVPTCTAVADDGMDSTNAQDTPSKGYPHHTPASRRPDADGTHGGRIATGSTSQNAAKRLRRSISSPAEHLSRSSVADAHAREMSVGPAHDANIAAFAMAGFQSSNRVTSRVVPPPEPSSISHVARRPPAVAAAVAESPQKRGGGCEGSLPSRKQSLRNVQSVPTPGSSELGSTSIAYTARDAQGIERETVSVGAAAMQRLDEEVPDARGWSAGGGIRTGAAHRPHEGSRVNDDAAENSTIAAGVTAATAASVLRVPSGLEPEPGKVLPLELDTYLLRLRAKSAAAGLGVAVGVEGSQGTANTSGGEAAGLFGMRRGGREAVAATGGVFPSGPSRVAASASAVQGQVQETATDAGAFSVDSSFPSAAAAATSGVFVSSNVAAAKAVAAAATAAATNTPVGAAVRTNGEGCSTTSSPSIEGLASLMRNLRRKLANMAEYNFGVEELTDDVTEGADDAAGDWLTDDEVDPEGGQFGDDDDDDDDVESVLLGMGLGAGVAGSYTPYASGGDISADDDDGSGQNIDTGDNDNDVTFGTYDRLYSGTYGTADIAATRRVAGAAAAGGTNAAGIAHREGDDGAPQAQARGCGGAGIINATSGGSARQFSGPWFAGSASAGSSDAGTPGDSRECDTFGDGAGLQGMEGSAYGNGNYSGSGKVRTSFLDRSRHPQGAAATTPGGKSSSPGAGRQVGTEARASGISCLLGSVNAAMLGSVGSARGATRAPNTAAPATDLVQEKVVTRPKRTPPPQSATVTARVPAAAGAAAGAIATTEERGCSVKSQPDRMPPTDQISPRKDSAVSFGRTASGKVPLKWGASDKSQWMGPRAAGSGAVAEADPDGCSFARARWASGSGFEQDAAGAGHTNAKIQNAEAGSTIPAAAVPNVSTIPTVSTSSAASPQLQNLRSEAMFARITQRLDALQNTVSQGLSLPRGTDDMGRAGSRAGSSASSGGGSGGGGYERGAEYRSAAAAAAAAAAWKGDASWRASSGGAQEARDSGSGGGSRGSQGSTLGTNNGDGGMRRDTFRSSAATPGAGAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP74</accession>
    <name>A0ABQ5RP74_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rubredoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000832</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00350">
        <property type="entry name" value="rubredoxin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47661:SF4">
        <property type="entry name" value="OS08G0162600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47661">
        <property type="entry name" value="PHOSPHOGLUCAN PHOSPHATASE LSF1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="2">
        <location>
            <begin position="153"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="231" mass="24419" checksum="1BF895B94682183B" modified="2025-10-08" version="1">MALMQRQAVGRNAFGTAKASRSVVRAARSRAPAVSVRADGQQIQVEIDKPLGLQLDQSTAPGGGLVVKSARGNAAKAGIKAGDTIIYTSSFFGDELWPADKLSFTNNAVTNCPSPVTFIYVPGENTKVNVKRLPKRPAPARFGRKLTDAERALATHICVDCGYIYCDPVPFEETAANYRCPQCNAPKRRFVAYDADSGKTQGVAEGTVGTIATVIGGLLGIGVLAYLGTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPI5</accession>
    <name>A0ABQ5RPI5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43689:SF8">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43689">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="108"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="366" mass="40309" checksum="CEAC89FB8D6F3A5A" modified="2025-10-08" version="1">MKTLRYVDHNILPESRPHNCVRAWRRVVAPCVIHAQRSASHLNRAQPASFIPPQVSEILEPAAQAIAANMRRVPVTISTIPGSPEVATAFVGPTRDEASRYPKDVPSIFLLHGFDSSCMEWRRLYPLLAAAAPTYALDLVGWGFTDSSLFQSDPSLVIRPEDKAAHMRAFLEAHTDGRPVVLVGASLGGAKALDFAHTYPELVKGVVLIDGQGFIDGIGKMSTAPRWLLWLGVKVLQSVPLRQAANKMAYFDKERFATQDAMRIGRLHTLLPGWLDANIAFMRSGGYAISGKIGQIQQDVLVLWGRNDEILDPSYATRFQNTLPRAQLLWVERCGHCAHLEQPQLAAEHIMEFVKGLAPAATAARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPJ6</accession>
    <name>A0ABQ5RPJ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RWP-RK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_001266</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription factor.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044607">
        <property type="entry name" value="RKD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003035">
        <property type="entry name" value="RWP-RK_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46373">
        <property type="entry name" value="PROTEIN RKD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46373:SF2">
        <property type="entry name" value="RWP-RK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02042">
        <property type="entry name" value="RWP-RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51519">
        <property type="entry name" value="RWP_RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RWP-RK" evidence="8">
        <location>
            <begin position="841"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="218"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="222"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="268"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="335"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="970" mass="102959" checksum="F4CF6A271676B090" modified="2025-10-08" version="1">MFQPEGARTGIGLAGSDFLWEGGFLDNSASDSVRVESSALAAAAGPAPSFRTSYYKSHPMDILDDHDEDFLKGLPPLAEWCPNHHNSARQKLAVAATVPRSTFLSASAQIVPDMYGNLSHWALSVPPALERASSRPAANCMVGALQPPNFPPVGSVGWTAFAPSLPVGQHAPNPGFAPEQWAPQPQTQAPPSQQFHACLGKDGPSEAVPTGSLPWPWGQDIEPSTGQASQGHVVTTGLIPGGHGPLPNSAVMVPPRPAITGSIHHRMQTPQDSHQPPQHQPSQQNQAALPPQQNPHQIPLQPRQRQQPQGLQQQQQQEQQQARLPHQQGQHHVHQQVPVRPQQNPSPQQGPPAQYSQQPQQHSNVQQCPPARQQHHHYQQQLSAQQFSLLPQHPPPPHPYQNAQLRLIGQQQSAPQCPPEPYSQQYMRQCPDGYVQPYSQQCPQACQHMHVQECPQQYQEQQQHMATQQQEQQQWHGYSEGLHTCLVSGQVVPEEELLMAEILAAPWPSSKMSYDATTAAYAAAAALANTQLIPMMSQPIPACGVVASSAPDPTAGCLPFSVYGSYGGGVATAGETMVASSAPATPMLSPPSPPEHCTTCRRLQAPVMMPGERPRGEADPAPLPAAASVPCPVRSMPQAREPQLAPAVVHSGPATFGQPLSFIDPRFTAPQPHHQAMGPLVDFHSQLPDEGMRAASRAVVLPNDVAVTAAVGASNSSGMGAAVLVTAASAAAAAPTEGPSEGPGGSVVRYEDAVHQMPTRASCQHYAGGGGGGGGISSDRKVGGHSGPAAAAVNAGGCFPANCPATTTANCKAGANAAAIESHQNCAHRVGGGGAAAAGAAAAAGAGGGGSSRSTNRITLEVLRSMFDFPVQEVVRALGISATDLKRRCRALGIQRWPQRKLMSLRRLAEAVETDREISEDQRKVMLDRVARNRAEILADPDVDLEACLKPVRQAHYKRNFVDRRGVPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPX5</accession>
    <name>A0ABQ5RPX5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF62">
        <property type="entry name" value="LIPASE ROG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="248"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="DUF676" evidence="3">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="478" mass="51768" checksum="56F1F98445626600" modified="2025-10-08" version="1">MHLVVLQHGVRGAPINTKYLENLLTERLGNTGFVFNFAGNFKFATYDGIDICGERLFQGVKSAHDNLVHQGTPPTHISFIGYSLGGLIARYAAGRLFSEGYFDHVMPLNFITIATPHLGAWRQTTNAIQRLVNLPAPEALSRTGTQLYLKDATPWGPPLLCVLSHPGLNFMAALRSFKNIVAVANVRHDRTVTFCSAAMQLEEPYPLRGFEHAVALVDPAYPSIVRPRAPGEIPGEPSPARRPLHGRLLLMLLTLMAVILLPLVLMMPLLALLHGRLHSRRKTTAVAKSVLQNLYTSWGGGATGGSVKQQSSLHGPDLPPGRPRTADVESGLLVRAGAGGNDSGYGGGTVLVTSGFAGGGPGTKGGLEDRVPLHKDHLRQQQQLFAGLPPASDKCGLQAWLVAQLATLPVRKVHVDTCNVLHAHSAIIVSNRLFRHCRDAMQYLVDQCMVWPQGPQGRSTDRQMHRQASSCQADSLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPZ3</accession>
    <name>A0ABQ5RPZ3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="217" mass="24337" checksum="0248827B19881C7E" modified="2025-10-08" version="1">MGIFHSKVVEAYRSVHVEFDGRRNRSILPTLLFATLIGGAGAAVYYHYEAVKKIFRRREEGGWKFYRGHTSSGDNLGQHVVRVADVRDECARVTSIAAEADRRVGVHAFDAAGRMYGHTENYPLLTGSIGNLLDGVFCRDAMVPPGWIAFPNTSLRDKGVKTLHRLSDVFCMAQQAAEQGMFMFDTSGSTCQERLISSLTYIKKSDRVTYVKYADRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ60</accession>
    <name>A0ABQ5RQ60_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF66">
        <property type="entry name" value="CARD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="112"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="529"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="675"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="727"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="789"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="936"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1141"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1206"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1270"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1551"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1662"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1773"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="875"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1702"/>
            <end position="1739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="541"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="565"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="727"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="748"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="936"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="949"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1141"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1221"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1292"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1565"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1677"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1797"/>
            <end position="1808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1808" mass="182299" checksum="6A7F1186C17022D8" modified="2025-10-08" version="1">MMSGVIPTVLSPARVSHALASVGPPAGSGGLRISSTDPLTPGNGIDTQQNGGGRAKSTSPSRAAATAFPISAISASSPLTADASQATTVPSPAAPYLTTPIHGPSGTTIIPIYRASGSGTGESSSAATSARSLNGRRPQTARSFRSRPDSPKTAGGRVLPAPDSSSASLSTAYGAYGGGGVLASPLQRALGSNGGNTRGSRGGLGRDRSFAGSVSSTTGQLRPQHGSLSGGFGSGLESMGAAVGGLARGGPMGLQPAPGTAGGIDIARLVAIQRLLDEYDSDDAADPGVTSPRAEGSSYRHRSDSAGGDSTRPQGSDSGSGTGSASGLRTIASLRSRKDMQHWQPSVSTAKGSLEEHQQRLRERADVTLHLKRWTEQVLEDARRAAEGGTSGRANEIVRAASVRFKDVPEDQGPSLTAALGEYLDPEQELDDEEERRMEALMALPPNPRGLGTQLRAHFMAVVEEEVNKKFDVMRNKWEAEKGIEAEELRELRESYRDLRRMHMETKEALDKANTRMDVMRLQMKATASISKTGKEGGSAATDTDTNTKSSLRGHRSSTHDGSEGKAGGKAGKGAGGHAAHTGDGGGAASPSPSPSGGYGHEDELHDKLRSIGSRRATSPTGDGSGATPGTAELQQQLKVHAESAMTLNRKLQDAEQQLLKLKLDKASLENQLTAARARGASPEPDSAAGTGPTGGGAGASAGKYDADVQIFKLKQEKASLEQQLAAAKAKSSGKVGAKGSSGGGSARSGSTSRALTAASAGKGAAASKTAELTADVERLQIELAAAKREAANSTKEAMERRMELQKAKDDLATTQKTLEMALQQIAGVKEAISQEKDRADKAVAAAEAAAAAQAQAEANAAAANSAVAAALIKRSAATGEVQELVERLEKERDAAVTAAAELRQQVRELSQSLGRQAEDVMQLREQLLQRQLEKPAPNAAPADAEVPPEVPPAASQPPPPAPGPPPVVAAAAGGIIPPMESKVTAAAMAAMQSLGLPLRPAPVAARRRPTSALGPGGSAGAGAFEGAVGVAAAAAAAAALVPAVLEVLQHRVPDMVMSLGGELEAGSAAAAPAEQPLADADGGGAASVNAAAAAAAAAAATLRESGNAAAAAAAGATAAAPIIASTFVDLVRAELMHSDANASAPGSGAAAAETATAGRSTAPSRPETIMAGATPGENLVSEQLDQLSALGSDRLYVFRRATPPTVVTPASAGGPSVSFADDSASPSPAATTPAGAADGVTGDRFSPPPAVVVDEVTFYPLGMRESLRTTRRSGAHSTILSGGHDLPPSPQRQTPSTPLPHHTPYFDAAAAAVAAAAADLIPQTVSVSVQSSRRTLVSSAVTAAARRRRSAFAQLHSNYLTPAVAAGSPPVLDPHSPHSTVLDWPIDEEGDGAAAEMAVTTAAAGGSSTGNRIHMIRSGGYYRDAADGALQDGYASVYVDPELASVLHVERVSPAAARAAGAGRGAISPEALGPSLRDRALSTLQAPEPLPAPLQRPQSAAVRTLDAARQFILQHGNGGGGRYSHPPHSLSAVLPPVAGAMPAAAAAAAAEAPGPAPAEGGGTSAAQPQQPGQLPLGLLMSGVKVRAVPANPQKGVQLSARLRAPRQPSARGVFGRSQAESVRFEDDEAAAAAGPSVSGRLVPGSGLGPAIREMQLHMGPQGGASSATYLVPSDPAGGGGGGGGHAGSGDLELPASGPELAALARGLMAAQERAVRELEEAMSNRRELAEEVLTSRQQWLDEARRARALEKELEGVRAVLVSLGIGAMSPGSAGGGGAPLAPPLLRASGVMGASTSGLPSLPSGGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQH0</accession>
    <name>A0ABQ5RQH0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dynamin-related GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001793</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF21">
        <property type="entry name" value="DYNAMIN RELATED PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="310"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="3">
        <location>
            <begin position="590"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="737"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="454"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="753"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="781"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="807"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="819"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="837"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="846" mass="91116" checksum="262089B57B5B5690" modified="2025-10-08" version="1">MAAIATQTIDAPTAGLEQVLYRLQEKRVALEKVVLQYGEAPAGIKEVFELCRGFERAYTNFINESPVAMKIKEAFLSEAGLAGKIKKLPMEKVFELKNVKSICRQADGYYPSLIAPENGLRELSNQALKSINEPVNICVQEVYNLCINAAREAAEKAGQFTEAALMGAMPMYVPDFKNVVMPAIISALDEWKKDSEKMAHMLVDMERSYITAGFFRHTTHNRYAKIKQQEQMRAAMAAKGIVTDPKGVISAAKQTAAKFFPSFGPGPGAPSSTSPGTPSPNGAVVDDDSDDGKSPPRPANGAGLNVSNAEDFIASYFDKLVSDDSARFLEAMKWQRRFFVFSESQRILYYFKSPEDVSKPQGLKGQVNIADCLVEDLDEKGNPRPPNAGPMSMTTQDKGQLMIRIRHRDPRGVVVKDHNGIILRAESMDTKLTWLAKLRKAAESRRPPNPPGAAPAAQQQQQQQQQPGAPAAPGAPAPGSGPSVVGPGPAAGGPGLGGPGVSVSGPAAASSGINFGMDDGDLSWAKEAEPVLDSHLGEGSSAFRADSVRDPEGRLLPAPQMLLNPLRLKDRMARATAASAPWEVRYDALLDQFSADMQLYISCICDTIIITVPKAVVHCMIRKSEKNLLERLFTVIHHLTPTQLENLLREDEPIIEKRKAARACLEDVKTAIFQVQQVLERQNMATPADRKDRVPLQALVFAYAGIREMLTPEQYNYFNAKFNDKYAPECARFNWYIRPQNPPPGGLQGGPPSRGPPGPPGGPPHGPPGGPPHHGHLGGPQPSPQPSSVPPLRPGGPGAPSMAAGGGPPPPRRPPPAAPMGTPMGAPMGPPMAAQRRPPPPPPGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQJ8</accession>
    <name>A0ABQ5RQJ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055225">
        <property type="entry name" value="DNAJC11-like_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052243">
        <property type="entry name" value="Mito_inner_membrane_organizer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44157">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44157:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22774">
        <property type="entry name" value="DNAJC11_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="15"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="468" mass="51550" checksum="934473B6BA9E5A5E" modified="2025-10-08" version="1" fragment="single">MDQSNSEHFFDDSAEYYAVLNIPRDASDEDVRRAYRNLAQVYHPDKHTDPEQKKRAEEAFNRLQEAYEVLSDPNRRQIYDVYGKEGLAAGFELGTKLDSVDDLKAKWEEFKRKQEAERAELLSNHRGVYMCRLDLSDPRASLAGQRPLFRAATVTNSVETPVGESDVFYLQGVAALRANAGAGSLIIGYRRALSQHDSLDANTVLGARPSATLTSTRQLTPFTTASLTTSYTVGQGTGMQISTTRHLPYNMQATLGWVVGPPAASGISFNITKRGTKYLVSGKLELGVATSLSAKMIYHVSPSVHLRAIARAGNTGIDLELGAGRKWGRNTAGYIGSVIGLQGVAVKGRLVRGGQTFEVPVTLSNQYSDYKALAAAYLLPPLACVVVSRYMVRPLVRWQRQRRERQLQQQHADAIRESLQKAFSERSLIEPVARRKARSEAAKRPTGGLVVLDAVYGKVEEYLAEGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRE6</accession>
    <name>A0ABQ5RRE6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M48 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002055</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07331">
        <property type="entry name" value="M48C_Oma1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051156">
        <property type="entry name" value="Mito/Outer_Membr_Metalloprot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726:SF1">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M48" evidence="7">
        <location>
            <begin position="336"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="533" mass="57122" checksum="3471224C08D56E56" modified="2025-10-08" version="1">MSRHGCMLMLEAASEGFRTISRCHVHLSSSWMTTLGTSEATTAKVTRATIKAGMAISVEGKGTSPGLARLSVGSALSSATAVAGMGQCNVPWVGRGLLCRLHSACNGGAAIITAAVRSSARRSTGAQAHAECAAVRQLTHMYGSGRLAAAAGFTAHAAPTGMHAWPRQRLATRPKLPVRMAIAAATGTVVGAAASGDMVTAATMTPFTNASGAVRCVWRDSRGYEHFQGRGHAWSLHNPRILYTTVAFAVGVVSYYLYCLETVPYTGRRHSIMLVSPHREQWMGKVGFEQFKALARAEHKLLPDLHPDVQRVRHLGSAIAAVAADGGGGGSFEHMRGMQWEFAVIDDGMVNAAVLPGGKVIVFSGLLRLLDRNDDELAAVLAHEVGHVLARHPAESMSTLNVWLLINTALRLTLGFALPDVAMYLALILPNSRKNEHEADVIGLRLMARACFDPSAATTMLDKLNRAEKETKQGRLMGPSLPPFLSTHPLTTDRVKLLQKDLPEAYKLFEQSGCASSRSDFRRALDAWMGGAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS84</accession>
    <name>A0ABQ5RS84_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="352" mass="35238" checksum="0F3188EBAFD5979E" modified="2025-10-08" version="1" fragment="single">LTGAAPPWPPNLPPPGLPVDESPPPPPPPPPPPPHLPPPPPPIPPPPPPPPPPPPPPGTPNMPMIPRSPLIPQAPLPPYNPNLPPDLPPPPPPPPPPPPPGAPPDVLPPDSPALPPPPPPPPPPASPDVPSVPPSPPPDLPMSPPPHTPDAPPPPPPPPPPPPPGAPPPLSPIVDSSPPPPPPPPPPPPLNPDQPTAPPAPDTPSPPLNFFFPNQPDGPPPPPPPPPPPPPQAPPPSDPSLPPPPPPPPPPPPPGAPDLPGVPAPPAEPPPQSPLPPWPADPPPPPPPPPPPPPLLPPPFPPPPHSPLPPPPPPPPPPPPSTPTNPLQPGAPPIHAALPPWQSGFDDPPSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS97</accession>
    <name>A0ABQ5RS97_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_002164</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family. CPR flippase (TC 3.A.1.211) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="603"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="653"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="686"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="718"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="744"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="787"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1633"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1685"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1710"/>
            <end position="1737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1749"/>
            <end position="1773"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="947"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="2041"/>
            <end position="2278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="382"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="494"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1324"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1917"/>
            <end position="1939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="494"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1324"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1355"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1918"/>
            <end position="1939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI60124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2431" mass="257349" checksum="7FD856D22BE40C6D" modified="2025-10-08" version="1">MAAPRKINKTRQFLAILRKNFILQTRGRRILLGIGGWAGLILQLLLPVSFFLVMYLPKYYIKPIRHGESLGQPPIRIESSSWAAPYDGPATSYPSRGRASLLLTPDTPDVRGLGLVLARTLACPREPRMRICPSFNLPASFHCLFMPPYGLYHTQVRNTSAYGQTADGTVERMDGMHRGVRQGDGLWASGKKGEIGRWEHGTDDEGFLVAASASASGSVAASAAEELPYWRIHDGGGGEGGGRLLRSWRSRRLRLRRLQEKRTEGSGESDGGIGDGGDEAFWQPAAAAEHPAGGQPSDACADFATCISNPACYEHVLQNQLTFMPSQEAARHATVTDGDKWDAVVHFPRAAAAAKAAEAAAPSGALGGQPTAQVGGTAGLLQQHLRQQQQQEEEGVRDGSHAGGVISGGSGVMTASGGATAEQHWSSSELLSDMVVAMNHTDVPPTGLLLDQFDVLPTRGNGMALYRRYWFFANLQMAISRAVIGIHVAASSPPKPPLLSPPPPPSAEPPLQPPPPAVDPAARSARGAYEQQAVSGVGVEEPYGDSRRLYDASDVRHSPSQRRNWEANEQVAFWADSPYDVAIAANVELSYKAFPWPAVTEDLGAAVAAVFFQLLLVYAFLPSTRAVVGDIVREKELRLREGMRVLGLSEPSYWLSWGLTHWVLLALSGALCTAAGTYPFANSSPAMMLGFFWLYAAALVSYGYCLSTLFSSSRMAGTVVQFLYALSMLPGFVLPSVYPYGSWTWYAACLSPPSAATLYAAALVNWEKVAEGIRPSTLWTPITQANGFCAATTLWMLALDVVLYGVLTWYLDKVLPKSYGQRLPWWFIFDRRYWASKASGRGAAAATAAATGSSASGFASWRRWRQLLSFKKARNGFQSSGSGGGDAEDLMTCPLLPAGGSTTVARGGGFVGGGGGIHDSSAQSYVFGTDVQSVSNPAPAAAAPVAVEVHGLLKVFRTLTGESKLAVDHLDLQIDSGRITALLGHNGAGKTTTIHILTGMLQPTAGTAIVNGFDVATQMDQVRASLGICPQFDILWPDLTVNEHLEMYGAIKGYQGREIHEAAIQAATRVDLLSKLQCPAGELSGGQRRKLSVAIAFLGNPAVVFLDEPTSGMDPYSRRFTWEVIRQHRSGAAIVLTTHSMEEADLLGDRVVILARGRVAAEGTGMELKANYGVGYNLTLVIDPREQQQQQSYGTAQELLQPPTVVAVAASYGSARPDGGVPRLTAQSPEAAVMAVVRHHVPAAELLSFAGAEVVIRLPREAAAAFPALLRELDGPMGRQQLGVASYGLSMTTLEEVFLSITAAAELEADTRVDGAAAVAVHGATSAANSNDSTSTVEPSDAEPSNAASGDAPLARRPASGVAAAAPPVHPQPLHGWRLYGRQLRALLTKRALCARRDRLAVLTQLAVPLALVYLALWISSLQVREPSQPPLELSRRTTLFNRPLVWSASAAARKASEGCNVSGGGSCLAALLAGHPAKGGAIDSGATALYAGDPRVRPATLEDYLLRRWYGGSGPVYDALHVTGLPPPATVASPVSPSEAAAAAPLSFVLLTNQSAVSALPAALAEAMTAAWSYTNNGTSPAPPTADSVRQGGGVNATGHRLSSSFFSIVSWPLPLMATEPLAQIEHNSASLMLVLCLVLASSVLSASFVIFIVREQDNNSKHLQLVSGAPASAYWAANYGWDLLSYSVSAAGLLALIVWYRLPQYNGLRLGAVAALLGGFGPAGISLTYLLHFLFRDEMCALQRLNTAYFLTGYLGFIATWICDVIEMFVWQPGALHDWNRAAKWALRTASPHYCFARGMYDVQATYNVAGGPRSNPFSWDVFGSPMTHMAIQTLAYGGFVVLYDGGVLTRLYVYGKDAAWVAWQWLRRDKRGQLFGRDGQSRSSASREVRRRGWWVDGWWRSGVQQQHLNININVGGGEGGDGGGGGGGGGGGGGGGGGGGGGGGGGGGGGINTAAGDMEEGVSLMTSAAAEAHGASRYSLNDDGRRALYQDDFNGEPGRYPLYGGGHAVLDSERVEDEDVVEEKRAVIAGLRNNCQVLLDGVRKSYSQGPFRAAVAAVRGLWVGVPAGECFGLLGVNGAGKTTTFRMVTGEVLPDAGDARVGGFSVRTQLAAARRQLGYCPQFEALPGAMTGHEVVAMYARLRGVPESYVDELASYLLRRLGLEGSARNPCGGYSGGMKRKLGVAVALVGDARVCALDEPSTGMDPGARRALWTCLQNEVIRVGRTVVLTSHSMEECEALCTRLTILVAGRQRCLGTVQHLKNRFGGGYVLEIKTLPRSTSGTSLEAGVVQAPYPSGHGYLGATGGGVAGQEAPYVQSATSAVQAGSGAGAETTEATLAAWLASVCPGAVLSHVDQNRYTFSIPRALGGGTPPGGPSLDLAELFEAVESRRAEMGVVDYCLTQTTLEQLFVSLAGAAAAATRNEEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSH5</accession>
    <name>A0ABQ5RSH5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">intramembrane prenyl-peptidase Rce1</fullName>
            <ecNumber evidence="10">3.4.26.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_002715</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.26.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.26.1" evidence="10"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="13">
        <location>
            <begin position="232"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="116"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI60557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="390" mass="42401" checksum="EDFFF823757C809E" modified="2025-10-08" version="1">MPSGLALAVLTCLAIASSYVGALYIWGRSLPRDHPTTIKRRLLSVVLVCGVSWAPVYFWATRLHGAPTPVHQLLGLRLKKLPVAILDSLLLVATLFAGPLLYFLYTLELPESHSTPPIMQTRGPTNAKSLNCNIKDANNDDQSGGSSSDYLDRDGDPDGETADGAAGRLSNAQAFATSTCRLRRSGASEGQRSSEPCVPAVGGGGGRNLGDRAVYAAGAPPSSWLHSATSSQSLVLWRNLVVAPLSEEFVFRSCMVPLLVLEGLSFQNVVFLTPLFFGAAHLHHVIHLLRHQQVPLGRAVATASFQFLYTMVFGWLATFLFLRTGHLMAAAAAHIFCNWIGFPPFADMMAHPRCVQLMLTTVAGVTAFFMLLPRLTVPEKYQQDYCFYCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RST2</accession>
    <name>A0ABQ5RST2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1192"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1192"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1209"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1222"/>
            <end position="1231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1294" mass="133679" checksum="F7AEB67A36443EA2" modified="2025-10-08" version="1">MTNARIARRSIDSLSRAAEHVEQAIAFGPEARYLHEKLADLRTLLEEFLTGRYQDAALDEALEAIEAPLRTFCMDSHSCCTLGPLETLLTAEERLHRLVNSCLRIRQGLTKLLQHGQLPTVVRAQANLISTQLSTVTVNFETLRQLQAVRLQLLEEAERVRLGTIADGTFNARIHRLAAACSAQQLAADTAIVAHLLEEAEAKQDIVKSHFLKLALRALDALRASHGTEGAELAQCTSPVDKDRRVIVGGSGGGDRAQATGPTSSTRASLSPTVVRVEQTLDSGITSTLAQLNIAAAPQAVRAAQLDALCHALDGPQRVQVAQALIEVDGMSSVLRLVATSMPGQRSAPLRLLAALVNAEPTCSGELLAARGVQVLLHIFAGYVHEARQRADAVSVLRQLMEETASVASTTVNMAGRFPARQKGGPSGGTPPVQAVRRGVGAESSPVGVQAQQQQQQQTASLVAGENAPAGGTELEEEEVCLLIMGLLRQLSLQNAAELVAAGAVKVLCQGVSLHGHGRLASGAATTLSDLAYDTAAVVQMLQERLLVLLADLRLSRRPSEVSAALACITAILEHPAKLPEQHQQHSPGHSQVAQLCAEMGRACDAASVTTLLDLAVDAASAGETPNRSTSSCPCAASLEPGLSRLLPFCVHNTETRTAVLRHSATMQLLLDRVAAAEANAMATLRRVANDDHCKTAVARHLAQLLITKSATHAVRRNCCGGGSGGGSSAAGSKLAEAAAVTLCLADVLRPASRDRSVLRWSSDACSECEQCGLIPPLLEITASPDAACRAAGLACLHALAVYGTQAVRMALGACGAVMALVDAVKLEMEMVPSEQGPQRPPICEPRTLGQAASEALAALATASEALRQDVVMQLAAFLGSSSLHLAASACQALLVLLEVCPMAGPLLLGHGVMNTLVAHFPKQTVPPPAYAFVNDASSMAVPTAYRSRGGGGSISDPAEAAQLLQQRAVRLVWSLCRGGGAAADAAVVAGVAPVLLQRVTSATITSLALRVESAAALAPLLKKSAAVREAAAAGGGLQALLHLAASGASRSDEVTQWDEALGAVAAMAALHAPCRAEAARALRQMLMRGTVREAAAAAVVVERMCSSAAGREAVLAEDMVEPLVRLLSTGGDRTKAAAANAISHLAVPPKATLPPCAASPTGGTVRPRLVLIRAGAISALLALLREAGGDASSSASSTTSTQVSCRTASSSSSGASSQASPVHSNGTQSSPADMALVAAVEALESLATGAEGVQAIRNAGGVEILKATVARGKRRLVPPAVAKAASATLLHCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSZ1</accession>
    <name>A0ABQ5RSZ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">HIT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_002581</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for box C/D snoRNAs accumulation involved in snoRNA processing, snoRNA transport to the nucleolus and ribosome biogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the BCD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23023">
        <property type="entry name" value="zf-HIT_BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051639">
        <property type="entry name" value="BCD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057721">
        <property type="entry name" value="BCD1_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483:SF3">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25790">
        <property type="entry name" value="BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="9">
        <location>
            <begin position="56"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="295"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="352"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="475"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="545"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="557"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="595"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="645" mass="65844" checksum="A173ABCAABB49648" modified="2025-10-08" version="1">MDTKAVAAAAPMPVLPCQKAACTSTPSSFSGHKPESVVQGAAGEEEQEERPSAPLCEHCGAAPSKYRCPGCQRRSCSLECVRAHKASSGCSGQRVRTAFVSMQEFDDRALMSDFRLLEEIGRADDVARRCRPPAPKQQLPPHLGSLVYQASQRSVKLLLMPPGMAKRTSNTTRFDSRKRTMFWRVEWRFPEANIHCVNERVDEKSNLGEVLSAHLRHPAPNYIPLRRYAKAGLDQLRVFMRKEKTPANQAAFFPIDITRPLASQLESKTLIEYPVFIVALPHEVGLYPPPAMIAPPPRQLKPPPAEAVQDAAQDGDVDGSGTAAAAAADVHSPALAGEVIIGVRPAGQWAVKGSNASSADGAGAPCGSPVPAAAGSRREVPSVPSVQLQPQRPHPLSDHATAVVAASTSAAAGAVAVVASKFAGTGPKGSSPAKSLYLGGGGGGLGWPSSSGSGSGSGDTAEGPPFKRQRVAVEAAGGTGSVCTQPPGSGTGSEIRMGPVPGLAKAGSTPFRTVSVAAAAAGGLGRKEGEASRDENEIELADEDENEGHAMGDPGHGADLAGVGARAAPAEEGAEAVRSGNSERGDSGGGGQGAGHAPAAAAAAATAAEGGDARSIAAVGGGGGTVAGSVVKDYNEIALDDELEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT06</accession>
    <name>A0ABQ5RT06_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable pectate lyase C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002698</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045984509" description="Probable pectate lyase C" evidence="4">
        <location>
            <begin position="24"/>
            <end position="2188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1473"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1603"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1671"/>
            <end position="1697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1753"/>
            <end position="1772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1784"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1816"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="1834"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="963"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1058"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1204"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1331"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1918"/>
            <end position="2007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2035"/>
            <end position="2095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2162"/>
            <end position="2188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="970"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1058"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1091"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1271"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1925"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1943"/>
            <end position="1963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1982"/>
            <end position="2006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2045"/>
            <end position="2062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2073"/>
            <end position="2082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2178"/>
            <end position="2188"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="2188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2188" mass="226275" checksum="40D766A59F6C1A2D" modified="2025-10-08" version="1" fragment="single">MRRRLLALEWLVRMLLSLPLTISVPYVDPSRWDPIANGVKGGLLSNLQEGHGRRLVMQPLQNNTEYNGLVTALISTITISNVTFNNCPNGALALTVNTSSSFNIVIQNCSFSNNSNALSISVNGTTYGGNIAIENCSFNSNADALSISASSNASIVITIRNCNFYKNSNGVSNAGASSSSSSSLTGAAVILSGTPTVSILDCSFQNNTAQSGAAVYSTVGNAGTGSLEIDNCLFKANRGQGAVFVDGGILYSVANSNFSYNTGGSALYMSELAFVSQRTVSIQQTVFSDNSADGSSDTTSSTTSDFSSKGGGILLHKSLASVYITKCTFTRNTAKSQGGGLVVSNAGSDVFLTSAVFSNNDGGLYGGAMVVSTSSTVIATGCSFTANRATQGGAILVSTISTLELAACTLENNTAGKFGGGISVVTTAVISVSNTTFLRNQATYGGALECWSCDYVSADRNYFLNNSASGSAGALGLYSASSTVSVKKNVFRGNSAARNTSSFTAKDCSRSTTGAGGAVCASITVAMDVSGNTFDGNQATSGGAMFVTRQCAIMADDATCATARLNLDGDNILKNNIAQGGGGGAIYALNVTDIAITCSGNSTLYTGSVLSAWPYGARGCPNWNGNQATAGGYGSGLATGLAGLRELPDNGTSRSNLNSNSVVTVVLSLYDFLGTAITKGTYEATGTTAAVWMTSELLQYSISGYSNSSDNGVVTFNTLKMKAPAGTYNMTIYALPTGITDFTLYEWANISLVIRPCKLGEVLDTGGDLCTECSGTSYYTFNTENKTCDICPSYALCSFNGSYTNTSDDNAGYLVPEDGYWHSSPFSPQMLACPYSAACTFDDRNVTIGTYQEDYLSGSDTALPTPNTSVLYNITTYWQLQCNTGYRGRLCAQCEGPEYGWRSSGRCYKCPARILNLLFYILSYMFTIFLLAFTVRATLGRGSGESRALVRAVRNSTRLAVARPVTNDKDDADGDDEDEDVEKEKRERERNAVAAVSFKIRDTAYVGNGLNGGGAGGDGGRRVAGLASFHGFPAAESSIEDGSGGGIGVGGGGAGMDLSGSGSGSGSKRGNYATDGASMKRRSRSASVSGNGRGGSDSGRGSRKGGPAGILEMAAAAAMAQLEEEAARKGGPFGDVNGSGGRHDDDCDDDYHTYGSVDGGRGGFRSYNGGAGYGTVRSAGGRMAGGEFAGALVVDAREGAVALPPAVLPGTRPNIRGRVPRSVRARESRLAQSSGPGGDGNVAMRISEGSDGSDSDQRRRHRLARMSRAEGQGGGGSGSDGSGSGSGGGGVSAGGGGGSGSGSGSGSGSGSYAAAPGKAFGEPVAESLTTWVDGMRRRRRGDGSEAVVGSGDGGGGTDDGKTMLSGRHGGGGASGLFLGPVGTLQREQSELRAQLKEDELDREDKPPHAVVLKILINFLQVTTVARNLDLNFPDFVTKMWNVGSMASSAMTTLVSIDCSFPENVTSKAIQRTILYVAVPWLFILAALPIWILLYMRHLRRRRKAMPELPATAAATTAPAGPTALAAPAAPDGGNKGYEVVLSPVVSNTKINGAAGQGEVTTAVTTTAMGPGAARCDGSCGGRYQVVAGEGDVSTAGPDSSETFNMYLSTRMIVTTLTVIFYFYPMVTDTVLYIYQCSELDVATGPYAAYLQSVGFFWELDYDLRCFHGPHLILTLVVAVPWLLLWCVGVPLGSALWLRYNAARGKLNQSDFSDRYGFLYEDYRRTTYYWESVIMARKLVVVGVTLAANSADNITQVLAVMGVVVIAGLLQLYVRPYALDRFNSLENISLASTLIILYLCCFFLMSDITDTTQKVISVLILAVNAATIGWFVYCLVVEAYRYGVQMLDNDGDGRVSKEEINEWLRLTLGKPVARVVAFVAARQLRPQQHPHGDGDGGNGGAVSVVWRPGTEIAMAPTAAAVVSPSTQPPTLPPHPLVVLGQPSPSVPSLPPPFAPLPSAAPPVLPQQQPWISGPTVATQVRVSPPPTQLPPPSPPSPPPLQQQPPVPTQVVALQSDEPADRSFTVVTDMQSSQDLIPVDSIPASPPSGAAAAASSPKATGANAKGNGSGLYGTRRSSGANPEVSTAAPGRQVSNGTAYGVVAPGSGAAAAAAAICNGTNNAERVGSIEAGALQGRTASGRGDRYPTPSGAAAAGAAAALLAAPGSLPGSPLESGKGNMLGSNSATSTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU14</accession>
    <name>A0ABQ5RU14_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">2Fe-2S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_002802</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="8">
        <location>
            <begin position="38"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="144" mass="15324" checksum="7FFAFB0FB7A251E1" modified="2025-10-08" version="1">MFSTKLATHPLPSADRPLGVRARSRQAVRPCATSTPTIQLTVQSKDGPTVSVAVESGDILRTVLMAEKLDLYTTWGKIWQCGGGGNCGTCVVDVREGADLLSELTQAEKKKLVGKPATWRLACQTRVGDGESTGAVTIATRPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU85</accession>
    <name>A0ABQ5RU85_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003434</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SFH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="4">
        <location>
            <begin position="103"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="295" mass="34119" checksum="8375083520773E97" modified="2025-10-08" version="1" fragment="single">MWSSLWSSSTTVAESYAEADAFWYLDPNPEQQAAMMELRSRLQQDTLTVEGHNDDYTLLRFLMAREFNVDKALAMYRDMRAWRLENHVDGLYESDPTGSSYPQIEQLLKVYPHFYFNTDKFGRPVYIELLGRTDANALFATINVDNLIRYHIWTWERYLRCYLPACSAAAGRHICTATVIIDLAGLSLMNFNSATQKLLTTFSKIDQDYYPEHLGTMFVINTPLIFRGVWAAVQPLLQERTRKKIVILGSDYLPTLTQMVPLERLPDIFGGKMRLDPYKSMGPWQQQQQQDAEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUK3</accession>
    <name>A0ABQ5RUK3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045438009" evidence="2">
        <location>
            <begin position="27"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="434" mass="49179" checksum="AA1871C53AFF874A" modified="2025-10-08" version="1">MTFKIWSRTGSMHALLVIFLFWGAAATRISNNLRASAQSIKDDLNQPSILDLENEDQALDGVSASRQRSGFNPKVQFNDAGEDDEDDDMDDKSFLLLGASSDIGKGGSDDNGKDFCLKNKYRKRTFKVVKEMPFVGLFPELKRTRRFEGSGITFANGSYWVVFDSLRSLGQVDLRFSFRGPLNKLVGPVGEESQFEGISYDNVTDTFFVLRESMEHEEHGLVPLTEELRLTESGNSYEVLERCIVPFALQDVNKGFESLVVFWRDGKRHMLGLCESNFCKTVLGPDPPGLQRGNGRLVWATYHAPEDEFDECRWDVQKVIHLPSDAYLLDYSAISFRGEFGTDVAVVSQEDAAVWVGVFDWKEMEFVTSNTDGTRMDRIYHFPRTSECRKQYCNVEGVAWIDGERLVVTSDKSKGTQDFRCMEKDQSLHIMALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUN4</accession>
    <name>A0ABQ5RUN4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">MLO-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003688</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MLO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004326">
        <property type="entry name" value="Mlo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942">
        <property type="entry name" value="MLO-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942:SF52">
        <property type="entry name" value="MLO-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03094">
        <property type="entry name" value="Mlo"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="844"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="883"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="408"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="530"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="737"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1081"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1176"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="356"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="411"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="570"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1081"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1103"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1180"/>
            <end position="1195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1201" mass="131731" checksum="B34D7CC800E66E42" modified="2025-10-08" version="1">MSSTVVHPSTVVEGWRMALLLLAFATVTFILEKSIHSLEHAFKNRRGLTIALEHIKNELLFVGSISLLLSAFQNLLAQICIPKYAQTIYTDRRRHLLAASSTTFCEPNKQSLWPVSLQHDTHIFIFVVACTHVVYGTITVYLTIWRVRSWRKWEDQSMEQARSGDMKVLKVPPFVNVPTKFLYRNVGSNPVIHFFLMLFRQYHLSVNQTLYNNARVLFIEQNGKDLDFNFHAVVIKGLEDELMHTLHPEWHLWIIAVIWYAIPPPAYVSFWMYGIALLIIMLVGGKLVDILVQIAVQVVLKYGESVMYGRMDDNPLAHGRIKALGRSSMRTIESTKPSFQQQFMEKLDKFSKLLEPATRDGSDVTEQRAGGPSSLVQGTMASLGALFHIPSGEMVIDMLRPGTSITREAHGQAQGQPQPAEKAAGADERSLTTPATPGASAIAGEPPMGKKLTGADGGGTAAAVESTTHQQHMGLKRLSAPAELRRQQGQRFQSPRAGTLDADNTNDNGHVAHVVPPVAKRHMSIITEHSTRHHQLRQQQQQQHNHHGHHHDPRDHDRARLNGRGDVQHQHQQVKSGGSPSRASLDQLEPARPPVDRRNSAFEMFQHHPGHRPRALSIRHALHNILSARWEPNTSPQKQQQLHQELLEMGCGRLQGDTVAPANGTIAENAAVENAAVENAAVENAAVENAAVENAADADAVLADGSTPRKGNKSEVSVAANRITAEITSASSGEVAVNSRPLSGTNQLAREPSNRRRPKAFPATSGCYNCESFSSGDEDGVRRGRSILRPIRTASGRRRKVPLLMRPFYCGTYVKGSREYVQDMLGKSQQLDAMELFWLKRPRFMTMLFTLAYFQNSLSIAICIFALAGMQDAVSTWQNVPKWAIILQLFLDVLLVPQVSLSILPFYALIQPLGSHCSKDIIEYATQKDEHVKHGKNRTLKGLWRLTQSNYAPTSAQGRSILVRLGFLGPKKFKRESPLPPAAEAPAEAPLPPVAHQLSYRLAMKTDRSKSFAPARTSSMSPFGKVACVRTASAAFPSRFKPNGTSGGAVRTASLRASTSVSSTEPQPAALQLDVWNPISDADRAPKRADSVGHRTQAVAGDQQLIQPQVQVVVGRQSEREREPESGAEPEPDAGQGPQTQADVGRPQPTEVLAAAAGAAPGVKCAHIADPLMNQCISTSSSNNSGVGVSGVSGNRGLDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV16</accession>
    <name>A0ABQ5RV16_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAM86 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF157">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 12 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="51333" checksum="7AA101FC19222E9F" modified="2025-10-08" version="1">MAGADEADFLELLVANSRIGDWVDRIRAHGNPARQKQALLLLQRAVESRSTYAEEARQAGVVPLLTRLLGLTDDEEVLDASSAIIAACSGPMATAGKVSAFTYDNAVVHIREGALGDGLGAKVWMVAHMLCLELASNRELVAGKRVLELGSGCGVCGLAAAALGAGHVVLSDVEGPVLRNLRACMHLNHKAEGNNATSAVAATAEAVPAAAGAQPLPSDCALFDEAEEVEDFDDMMADMMAVDGRGRGPMTHGSSAPAAAGPASTSACMMQHHHDDRQLQQLQGGGDDESAWEVGNTSVRLLDWAESRSLLDSGVALEQLTVPPAADDAGAGGNAKSRDVGGGAGPGGISGDGEADMPPGVPVWERFAVIIGSEVMYEKVHAELVAAVVKHRLAPGGCAVLQCAVRELKVFEHFIRECRRRALRYRSRQLDPVSVFAPGRTGGGDGGGGCCSFEGILGRAEEYEGGVLLIAIDHEDSPCLEWYRDDWETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVL1</accession>
    <name>A0ABQ5RVL1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17167" checksum="A82D4A78B8A27506" modified="2025-10-08" version="1" fragment="single">MSSEHDDTPHTSLASQPDFVVAARAPDRGLETVGVRATQEPLFLQTPAHLIVPHSAVNTNMDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTDKTPGHSIADATRAYVRDAKKYLQAVHGGDNDGIRCLFVGNTLEGSAKDWYRNWTNAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVM9</accession>
    <name>A0ABQ5RVM9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003758</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045435104" description="Peptidase M11 gametolysin domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="3">
        <location>
            <begin position="172"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="594" mass="63623" checksum="B2F8DDF33CB972D3" modified="2025-10-08" version="1">MSIRAICLLLALAVLSSAGLVDIVGGQDTPTKAPGKPLRSPPPPPDRNATNVTFMGEIKYVDTHDGHQSYTFYDAEGNVTPFARDLVLPDVDNKTVPISTGDVLQLECVVGRVCSSCPSGENATCICRSEEQIQGCLSTNQATVSQVVPAWQVAEMNPNNPNEGTAINKNYRLLVLILDYTACGFAPSLDVNTVTRLYMGPNGDGNGGVAEKFKQCSYGKFNISYFQAAVIKPACSTAVTSGCSWWAISSAADAAAKAQLGLPFFSNFTYYTYVVPPGLQSVCPWAGLALLPGKQTWLQSTGYGVLRWATVMQETIHNFGLWHSWKNGWEYEDYSTAMGRGDSCPNAAELSRLGWATAAAQGGEINTGILTAPGAYVTWTLPATYITGDNNYLRVIPNWLPTYGNNSLAKHLYIALRVPKAGDAGMGSTYSNRVHVHEVNATMDNGYPGSYTFTDRKIQIINMIPQNTRQVISGYNMVVFTGELTSNDTIQVSLCRFDGSDDSQCSPPSPPPAPPPPLKPRPPPPSPQPLKPPPPSPKLLPSPKLLPSPKPVPSPKPVPSPKPVPAPKPPPPKPVPSSKPPPPKSPPPSAKTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW56</accession>
    <name>A0ABQ5RW56_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NlpC/P60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47664:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_14, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47664">
        <property type="entry name" value="NLPC_P60 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="32811" checksum="CA7EF1FFF1669FAF" modified="2025-10-08" version="1" fragment="single">MRTPASVIVEHTLCKSNEVKEGPTLQEAFASFLGSRQKKSRARTAWEASETRPQTRPASTSILAQEEKRARVRALFVETARGFIGVPYARKYHNRHYCNCEGCSASGRQLYHSPLFLDCCGLVRRVAWALQRQLGFRLGPGNQAYQYDTLPKRVESVEQLKPGDLIFYSGTYYEPGSRRHAFDMTHVEIFVGGSSGEATIGSRERYKWVMQYDNYRFESQRWKILACHFCSIDTWLDGLCHPKHPELWRPRRRTKQQQQQHGSTSSEGAWATVRLTRGTRGTGRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW98</accession>
    <name>A0ABQ5RW98_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anaphase-promoting complex subunit 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024990">
        <property type="entry name" value="Apc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041221">
        <property type="entry name" value="APC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12827:SF3">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12827">
        <property type="entry name" value="MEIOTIC CHECKPOINT REGULATOR TSG24 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18122">
        <property type="entry name" value="APC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 1 C-terminal" evidence="6">
        <location>
            <begin position="909"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="350"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="819"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="350"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="830"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="854"/>
            <end position="865"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1328" mass="135093" checksum="0709F51531B62069" modified="2025-10-08" version="1">MLNLSITSPAATVALALMYLRTNNAAIAGRFQLPDTPFGLDCVRPDCITLRALGRALVMWDAIEPSEDWVEMNMAPLFRGPLERLVDRVVAQLHGAAMGGPTGRARGVGDGLRGGGGSYHSSDVHAVALAHVSALSGLCLAMGLKYAGSANSRAYETLRWVALALLAAKRRLPEVAASSTASGGAAVVTGAGQVSGGGAAAVGRLDRAPLEAALNVVVLALSVVMAGTGHLPTFHLLQALSNRRHPTHHHVLHSLGVGYGAHCAVSLALGFLFLGAGTHTFSTTNSSVAALLVALFPTLPHTPTDNRCHLQVFRHLYVLAARRRCLEAVDIDSQQLVYVPLQLTVTPPRVASPSVPAVSTAAAARGNGPTGQGSGADGGTDGDGVAGGDVMLDDNTVLKASTKANLPWSPDAHLRSSIGHAGVPPIMGTGSAAGATASGAPGSPPMLEALHLEGDSPGLASLLRAATTAAGTTGHAAGAAVPKSSPGAVGAMGSPHVPDSIRVGTSCAATPPFMNTACGLAAAGTGQPAAGQSQQLAVENGQVITYERVAPALLPEPHQTIRVAVRGPRYWPQQLDWSSRGTTGIDGTSAAASYSMAASASAAPFFRSNSISSEGPPAAAGPGGLAASAPSQTPYGQFPFATPLRVGGAWAECNMNVAGLRAAPPPASGVAAALPTSLPQAATAGDPFHNTARGARAPTAATAGGCSALETVYRQLLLFVKKRAGSLSYSQDPSGIRSLLSRAFYNQGLLRMPLEHSPPCTGTGTRSRTAVVAGGAEAATPLNVAGVAGVSPNSGGGAIFGARRQLDWCTDRHGDRRAASLGPYSPPCGGSAGNGGGGGGGGGEVMEPPPLIEGQHERDQCRADREDDQTEGSGSGGGNAARAAAGCAPTGIGDGDRTRGGGGFDLVHLCATFSVDPSIMAFAQHIKAMRDLWIAVEADTEAAFESGNVTAGGFRRGIVLLAPVSATPPAQGIDVEQQRRLSQWQRRQRRRHGADLLSFCYSALYECITGEKTAALPMYLNLHCLVHQAASWSSPCAQLGKGRFRPLSHGLLGAIGTAGCRSDPWRISVASAEGPMHPGSLLTEGQVPLTTALRGLQLARAYYSGPLAAAAAAAAAAPAGDVFVDLDEQALWRPLMQPAFLDSLWCTLQHRRWRPMGLLPPQGAATNIAPSVVSAAAAAGRVGCSSSSSSSSSRSGSALEVYLRRGRTLRSVDFDCVVPGLSQLASEAELQADLAACLALHGLPPLATVIAALSQLRALPAWQQVRGMLQPATLPTTGGSGAIGLVGIGAGGGGLGVRSSWALLPLLSLVMPGLSNEVLLLLAAAITT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWD7</accession>
    <name>A0ABQ5RWD7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004162</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03704">
        <property type="entry name" value="eRF3_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04089">
        <property type="entry name" value="eRF3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="134"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="78"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="567" mass="62377" checksum="4410CF48AC22C4AA" modified="2025-10-08" version="1">MADTWEEDAALQPPTAPAPPPTAPTLNPNSMIFVPGQGMVPAFRPVEAVPPPPPVQPPPKVEELIPQPPQPEEPAPQPTEAEQLPDGGSAPAPAPSSAPAAPTEAASAPEEPVAPLDESAVMKAYQESLIEEPRDHLNIVFIGHIDAGKSTLAGQILFLTGGVDKRTIEKYEREAKEKNREGWYMAYIMDTNEEERVKGITVEVGRAHFETDKKRYTILDAPGHKNYVPNMIQGACQADVAILVISARKGEFETGFERGGQTREHAQLAKTLGVTRLIVVVNKLDCPSVAVDGKWDKARFDQIVSGLTPFLRQCGYNLKKEVTFLPLAALYGHNIRESVSKDLCSWYDGERLFQVLDQMEPLERNPLAPFRMPIVDRWKDMGTIVMGKSEAGFVRVGDVLQVMPNKLRVKVDVVFRDEKETQSARSGENLRLRISGADEADISPGFVLSSVKNPVPVVTQFEAQLVIVELLEHNPIFTVGYKSVLHIHTACEECEVSKLVAEIDPKTKEQKKVKYIKSGGICICRITVDKAICIESFSDVPSLGRFTLRDEGRTIAIGKVVKLPKVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX26</accession>
    <name>A0ABQ5RX26_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004783</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE-SPECIFIC PROTEASE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="381"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="153"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="242"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="315"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="833" mass="89693" checksum="271B3D1513DAFE98" modified="2025-10-08" version="1">MADSAVSPSARKRKLESSSFVKTLLSSGRQAYQSLVRAVEVVPKRTRLATGWCRFSWNLHQTAASDTSHQSSAGLILRGRHREAGNAPENEIEPSWKRGLVFGFLQAPNAETSTSIADYKGPELPETMSEVAGTDAGAVEATPAKPGPMLQSCAASTPLLPPPVSNPHGPEERQHQSPTLSSGDAFYGTATRALTQAASGALSSPLNLPLRPLSKLRAHGNPSSMTSTRGLVQVMVGSPATMGPCSLRGSDSTSITGDSLPAESSPPLLVQAEQASGRSQQEQQHHQEQQATQSLSPERQQSWRSFGRELVPLRQQQQQQQQQQQQQQQQQLSLRSACGKGPKVKTSASGWGRPAALLRQVHFPKPVEPLSPSAASPSGSVAISAAAWKRLQTHGECLNDEIINAFMLLLTKRSRDATSAAEVAAGPLHDHHKHSHQQQSQSQSPSHHKFHDNHDGSPLRVFAFNSHFFTQLTCGGVGGDGSGGSGGGNGGAFAYSQVQRWTLPVRTNTPDCVVSWDLLLFPILVNKCHWCMAAVWPRRRLLQYFDSLPGDHSKTADWVMDTLQRWLETDAADKRVILDERRRGPFSHGGAGRGVVSDADAQGWRRESLELQIPKQEDTTSCGVFACAFAELLSRGVQPHEFQFSQADVPELRCGMEAQLRAGSLDRTGEEVSLGDGGGDSCANESTRTFTVAEAVDGDLITITIPNPTMTSSAKVRSTCSCISSVAKAALDLTTNIDNDDDLNDSADGNLAAASEILGLIDEEVVDLTEAEEGDLRDVVDLADLEDLEDVADLGDVIDLSDAEDLPPELDDLPEANDLPDVIGLMEELSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXN3</accession>
    <name>A0ABQ5RXN3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004312</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="16985" checksum="DA1D1C9791C3352A" modified="2025-10-08" version="1" fragment="single">MASGTFHPHAGAPPTPLQPLRVDLTAAAMLARCSAWMWKGPGTWSRQCPQCHRSPPSTRGPARCWAQRQRRGDWARMPRARQRVVGTTTDYKNTGALSISFANGDSQSLPLVAEMLHSRQEVKPRRLNFGNVHLQSPKSCSWSCPVPPTSTSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXT9</accession>
    <name>A0ABQ5RXT9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004937</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="1088"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="100"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="344"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="761"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="356"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="776"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="790"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="818"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1170" mass="116269" checksum="8593FA9E94764F39" modified="2025-10-08" version="1">MSKHRRRGAEVSGGHSSTALRHTQLINERAHILGLQLQQAVLAGRIATAIRSYGFPTAQQQLHELLQRGVIHEDLASARAEVDALKGLLDRVQSGRLATTSAAAATSTTLRSATGRQAGGGGSGSAASRAAGSKQYGALAVDHVRHWLDQAQEAVLMLEELTIPGSIDEWPLDLPEPLPPRAPGGPPVAHFIQRIPPELAALLELPTASYDKPPLGAPGLCRQALAPTGAEGAAAASMQVSGAGGGGGGGAILWEAAPLLRDPSPAAAVPGAPLLYRVACHESAEHMWPSLAHGDLVLLMPGRYNWPEGTMLLRSAVRVLGLGRQPSDVVIHNNEEDDIFVECSAGPSPPPPPPQQQQQLGSGSSAAAFVPPVGPGGPSAAGGANATGCSAEEATGVVFENLTLLQLGGYEGVLRVLRGRTTLVDVVVQFAMGGIQVDSGGHLVLRNVRVAGGATAGLQVAAGGVLEMVSGCEVVRCGAGDDVVPKDLGGVEVMVPYVDYPRLASPSPRALLQAHAELLASGGPYVGAAAPTARPASAAALETLQPYLGAVQSPATVVIGPGCTIQNTRGFAISLVKRRALAAYHELLHSASGGGGGGGGSHSSYGGCGGSGSGGGGGGGCSLAAALDGARVFVSRGSLLGPNNSRGAVGVVELEYHQLDGDGVNRRTASRHFHAVQMSARQLQDGFASPGVDWTAHRIRLEGQPARPGRKATKIDVAAAAAATASGGGGGRRGGPPGVVVGSRTLRQLLVANVTRAQRRQRGLTMQYGGGLGGDDTSEDSTGDDDDGDMSVSSRGSSSSDGRRNIRSGSEGDGGSGRSSNDGDTAMASAEASSTAAGGGVGGDLASERVPQPPPPPPRFCACCGWSSRFACTVATSAVTASDAGIGEAGIPGAVSEQVVDGGATEASKVEQQLSEVKAAAAAAVAAAGLAATASTTEGADMAGGPLAYGGAGTCTATCTATRPGSAAGSGDSGDYGCPYTEPNGRLLVGSGGTCAVAAAVMLAADVSSCSITLDAPTPSVAMPAATSAATANPSAATAIAPGADVEGTPQVAAVTATALKACDAKAADAATISGAGAVELINPSTSAAGCAAAAAPAPASVGTDHRAMKLRRCGGCLAVLYCSAECQRRDWPRHAAVCREQRSLAAAASAATAANSVVKAAAKTCGKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY58</accession>
    <name>A0ABQ5RY58_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004968</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07379">
        <property type="entry name" value="MPP_239FB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051693">
        <property type="entry name" value="UPF0046_metallophosphoest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12905:SF0">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12905">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="33"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="263" mass="28704" checksum="9CBAA0BB0C788050" modified="2025-10-08" version="1">MDELEERNRCDTTVVVSLSDMHGLHLSGDIDVPDGDVLVIAGDIELRSRRDVGNLERWLASLPHRHKVVAFGNMDKLAYDACDDLEIEGAHVVVDRVLEVGGLRLLASPWSPKYAGVWQLADEAAGRQHWSRLLPPDLELDVLVTHTPPYGYGDLTRGIHVGDKQLLAAVQGLKTPPRLWVCGHIHEAVGEYRVPHPAAAQRPGGGILLINAATFYVAAKKGYERRAQPRAVSLPEGRVVAQGNPDNLAAAQHGFDGGRRRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYC4</accession>
    <name>A0ABQ5RYC4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mago nashi-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_005031</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mago nashi family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11295">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1560.10">
        <property type="entry name" value="Mago nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004023">
        <property type="entry name" value="Mago_nashi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036605">
        <property type="entry name" value="Mago_nashi_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638:SF0">
        <property type="entry name" value="MAGO HOMOLOG, EXON JUNCTION COMPLEX SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638">
        <property type="entry name" value="PROTEIN MAGO NASHI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02792">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89817">
        <property type="entry name" value="Mago nashi protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="17359" checksum="77EF025BCD2BFB6B" modified="2025-10-08" version="1">MADDFYLRYYVGHKGKFGHEFLEFEFNKDGRLRYANNSNYKNDEIITREVYVSQSVITELKRIVEDSEILREDDNNWPAPDRVGRQELEIVLGGQHISFTCTKLGSVLQVQNSKDPDGLRIFYYLVQNLKCFVFSLISAHFKIQPIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYN9</accession>
    <name>A0ABQ5RYN9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1995 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021503">
        <property type="entry name" value="DUF3110"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11360">
        <property type="entry name" value="DUF3110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="30719" checksum="CC3FE4BE91452624" modified="2025-10-08" version="1">MFRLAIRGQVAVRHSVFQPKRQTRCAPLCVRAATSTSGTDAGSASPSGTKNSAYVRILRYPDGNERRIIYPTPPAPEVEIDVEDSWSLWTLEDESSSWNECGLAGAACVVADPDPLLEELAMLQTRHPTNINELVKNSFQILAGSPWLLPRPLFVLTQQQATTGQATLYCLQARIRGEEVQDELAMVLKRPGLASSLTFSRMKDGVVAFDRAEDAARYATCLEADLPDEVEVMEVDSRQLFLLTSDVDAVVVYLPWSEFIPLPHQLAAALRTASDEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ33</accession>
    <name>A0ABQ5RZ33_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine zipper transcription factor-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">VAMT030</name>
        <name evidence="9" type="ORF">VaNZ11_005715</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Regulates ciliary localization of the BBSome complex. Together with the BBSome complex, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. May play a role in neurite outgrowth. May have tumor suppressor function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Self-associates. Interacts with BBS9; the interaction mediates the association of LZTL1 with the BBsome complex and regulates BBSome ciliary trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LZTFL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026157">
        <property type="entry name" value="LZTFL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21635">
        <property type="entry name" value="LEUCINE ZIPPER TRANSCRIPTION FACTOR LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21635:SF0">
        <property type="entry name" value="LEUCINE ZIPPER TRANSCRIPTION FACTOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15294">
        <property type="entry name" value="Leu_zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="156"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="328" mass="36635" checksum="E82727891CCD1335" modified="2025-10-08" version="1">MSISLSEDGELQVQAYLRFAKLKRDQHVREVISTVSDFRSEHIRQGELYSYKDLSQIFQELGQEAKQLIDKEIQNAYHTNALLVKILLSQAQAQGVDLFVDTHALENELLLKQISASEATALARSASDFLQRNITLGKLGTVATVTTQDPALIKERDALKAELVLVQERLIKLQEETTVMMRDRTALNNQINRLKDELATKDKELASVLGDKDASVSKLLKEMEQISATTANMSELTAAEVEQLKKQAGGLGVKLSALMEDLKAARNQVALKDKELRAAGEALNGKLQESKQFLQMKQMMQAKSQEVAMLRKRLEKYEPQNVPSADIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S057</accession>
    <name>A0ABQ5S057_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005903</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="144" mass="14366" checksum="ABD72334967F2A6E" modified="2025-10-08" version="1" fragment="single">DILKAAIREESEAAAAAAAAVVAKPPLVSAGSPPPAGVAAASVTALQKDSSSGSAPPPPPTCEETPPLPLRGSPSSSLDSEPSRLNDTGYITTPAQWHRRGPREFGGPATAQPAANINNIFLGGADGGGTIMRSSDPDGLYGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0L8</accession>
    <name>A0ABQ5S0L8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="391" mass="41850" checksum="0437766296DD7EC6" modified="2025-10-08" version="1">MSDYKKQADRLRTLRQIPQHQQSHSLQLPPREPVQAEYHRAPTNVNALVQRHYFPAEGAVSYGVGEPAIADISDAADAADRLSKYQSICHRRAAEASRAMKAERDAKLKLAVSAQERSQAAKAFGVRVALSNLKASPSNPGPRKAISQQDPGESGASCMGSGPTCPNNGLEEGPQGPLGCGEEATNTNSNEVEVGGSEPAAAATPEHISCGEAPVPPTALDPIPMKHGLDAPPQAARLASVSMSARLRLASHSKHAAMEVARVQVAEGPQCTANMAIEHPPAIKGTRNEAKAFKTRVLQPANPNLGAKAYKGKVLPVAISVTDVAGLSRVAQKVSEISEKLDLPRLCACPNNATIFDPTYVNKCARNCPLYHQPERYERLLTTWLREKNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S132</accession>
    <name>A0ABQ5S132_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="304"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="356" mass="37645" checksum="962C9B4398475D94" modified="2025-10-08" version="1">MPKFEELALGDDGDASAVGSQHQESNASAARSAALAKQLAISDQAPPWWAQQLSRASSLVFNGGLVALMRAWVNPPTMEVPEGGSVPEWYAEEWAQRTRWRRVGNQVAIDTAALVAVMLLMDGVEDGMRRFRGGAEDPWNRVMGGVTAGGLLGLLWYPGNPRGRAYMTAAGSFVGYFSYVTDKALDSSLLKTQRELERELLEKPDDQLRSDLSRPYLQSLLRAKQRELSNAARKAVAERVQEEQRGLAQPTLPTSGVMVTSSAGVAGSDISGDSMSPYLSSGEESFGSSVASVSGGDSVGGGAAAAPAGDNPANNSRHGRRSSSGLSYSGSSSISNDRGVLVITEEENEGTSGRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1B3</accession>
    <name>A0ABQ5S1B3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_006418</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="799"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="866"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="948"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1049"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1079"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="98"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="414"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="867"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1178"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="647"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="651"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="696"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="733"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI63446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1433" mass="158909" checksum="612C749432EF53A5" modified="2025-10-08" version="1">MGTLTKYDKASLGHENIRNDTFIERLFLAWVYAVVAKGRKGTITQAELRMPADQAAEAAACRFDAEWEKELVLRGKTGPDMKPSLFRALRRTFGIEVFIAAAWKITWSVFVLLGAFYFVRSLVQFVTPISRNGNMYNKSTIPHDGIGWILSCAFFLDSVLVGIALQRMGDECVRAGIKIRAALMAAVYRKTFKIHSVHEENVVSLVATDCAKLYEGVLHLQNVWTAPLEACAIIALLLYLTNGTYGLPALGVVVFVLPLQYYLGYRIANFKLETVEVSDARVQRMHEVLLAIKLVKFYVWEKSFAKQVSDVRAEEISLMSRTSVVKTINLCVVFAVPPLIALVIYATYVFNKGPFDSTFAFTVLSLFNTLRFPLVVLPKALRGSSEAIASLKRLEKYLLLEEFEDPPKSKVVEARFENTVLSYPGSKEDFRLQIPTFEVKSGEVVAVVGRVGSGKSSIFQAILNHMTVESGALHVGGSMAYVPQTPWVQNLTLKDNIIFGLPFDEEKYKKVIHACALELDLQILPKGDQTMAGERGINLSGGQRQRVCLARAAYHDCDLILLDNPLSAVDQHTARHIFDKCIKGIFKDKAVIWITHQLELLPEVTNICITDSGKSLYFGPYDPEALNRHLPVDHLLFATVEAGESGVQKPVDADKDAAHGTTAGDKDAVGSGTHSQGDSNGAPHGHSKQPSTLSDGWKHKDRSGTEGRKSFQRSSKAGIRPTDSVSDSLNKLAQEEAAKQEEEAKSHERRSADRRSGSRGLGRVSEEHNPGTLEETPEEIAALFNGVPRRVSVFQSFKVYVKSGGTLLFFISIGIFMLTQTVRILSDTWIRWWAEPDRYDFYPNGTFKNVKNLNPKFHSVQKASEVYILGYFAFVMFFVICLLSRDGLFSYWHLHGSTQLHNKLFYRVLRAPLLFFLRTPVGDILNAFAKDQDTLDETLPDTLHMSLIYLMILLTSLGIVTASLYIYAALTAALFLAFAMMQQLYLPAATILKRWAGETASMVFVHVDESLQGMEVIKAFDAENYFVQENIRRINVHHLALFNTEQCHLWLAFWCDFYGAIMVVATCLLSVAYKKEVGAAAVGLAISNTIQVLVFFTWVVRGAADTVSMWDAVERVATFITNVPQENDIAPAPLPGASNGNLQGLPNGNGNAASSSMVEIKVADDGEPPKGWPTKGDIRFEKVCLRYYPGAPLALKYVSFHIHDGEKIGVVGRTGSGKTTLLMALFRMFELAYGRIIIDGVNIASIKLQQVRAGIAIIPQEPVMFKGTVRSNLDPFGQLPDSELWRALELVHLKEAVTELGGLDSVVAEGGSNFSLGQKQLVCMARCVLKPTRILVLDEATAAMDLQTDALIQKTIRKAFTERTTVTIAHRLDTIIFSDRILAMAAGQLKEFDTPTALLQHSTSFFTKLVEDTGPHASKMLRRMAAEGPKDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1B5</accession>
    <name>A0ABQ5S1B5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006360</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasmic vesicle</location>
            <location evidence="7">COPII-coated vesicle membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF6">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="9">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="10">
        <location>
            <begin position="169"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="428"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="809" mass="84473" checksum="DD2D2E5737E4E733" modified="2025-10-08" version="1" fragment="single">MTTPSNAGLPSPNNSVTPQVAGQQQTNSFQKEQKHTASSQMQPAVTVAPADHVLTLVSSSVVNQRGFANTCGVPLGAHMDLRPSEALRLPLIATRPPLRCNGCSAFLNVYCKANLPKGVWKCYMCGAINIQKDLMEAANVTAFPELSSEAVEYSVALPPSHPLLPTPGPGHLVFAIDTTLEPKDLQAVREALLTTLANPCLDPSTLISLITFDGCVAVHNLGSQRFSHVLPTGSTSTGAVQPGSGSSLLNLQSVVQHLLDRHCSATASRRAGGGGGGVAAASSALVSDVESCVQHLPKVLASFRTVQSEVPVRERARCLGSAVEAALRLIAAHASLDLTRHHNLYSSRVIVLAGGPATRGPGAVPLELLDQAVPEKGRASDNRAVAASLDVGVALGDMASKIGVAVDILTSISTGVNAKLLTAITHGSGGEFMPHPPPHHPPPHLAPSSSTPGMPPAQSPQSQPPTQSPPFPPQQQPHQRQGGEVNDSHQQEQAVAATTTSGSGSGGFIGPLLSRQLTASLTRRFGLEGRLDCYCSEGLRLVQWFGPLDTIQVELEVGGPAAAATTAAPTGTSAAAAAAAAVVAATAAAARPLSSSATVGPYRLSPVACGVVAVESGRCASLRLEVTRDLDDVPSLFLQVAFHFVNPLSRQRVVRVVTRRLAVVESRGEYLRTVNPTAAAALLGKRVVLEGKRTGAYRDSRKAEEARYAVAAQLGLVATRCGQEVLASRGVLGFGGRKNRQWPPELMPLAYALYHFQRGPLLGPPVPPPPGTFPAAAHSHLHALWDSDARLAGFNALLRAAWADAYRSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1E5</accession>
    <name>A0ABQ5S1E5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="330" mass="36304" checksum="2B1F07466C42793E" modified="2025-10-08" version="1" fragment="single">FLKVDERGVVVALVHVDDTCVASDDLELVRKVKEAIGQCFKIRDLGEARVFLGMDIRREKDGDIVLSQSRYIEQILERHNMLDAKDRATPLAMGTRAAPVTDDEGKLLEDPTPYRALVGELNYLAVSTRPDLAYALSVLSRSMAKPTRAAMSAAKGVLRYLAGTREMGLRFPGGQGLQLDGYSDSDWAGDTVTRRSTTGYVFRVNGTAISWSSQLQRTVAASSVEAEYQALSSAVREALWLSKLSDDLKIGTSPSIIKVDSQGAMNISNNPMTSQRSKHIDVMHHLVRERVASGEVELRYCPTEKMVADVMTKALGEAKFKWCRASMGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1F0</accession>
    <name>A0ABQ5S1F0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">threonine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006749</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF44">
        <property type="entry name" value="THREONINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="349"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="743" mass="83011" checksum="CDD17F9B75E30065" modified="2025-10-08" version="1" fragment="single">RNKMRPILQKRANAACASCKRGALGWLRASQFAPTPTTSGPCIVLNGNLPPLGITARLQSSWSRSSPLLAATVEAPAASEAPLEQTSDTIKQSVFMELKTSDESEQLLRIRHSCAHIMAMAVQRLYKGVQVTIGPWTERGFFYDFQWPADSPINEKDLKKIKKEMAKIIKADLPFVREEVTPAEAEARIRAAGEPYKLEILQSILQRNASASITIYHIGEPAAPAAPAAPAFVDAPGAAAAASAAPSKALAPSGVKPWWDLCAGPHVARTGDINPDAVELESIAGAYWRGDEKNAMLTRIYGTAWETPEQLKAYEQLKEEAAKRDHRKLGAELSLFSIQENSGGGLVYWHPNGAMVRHLIETFWKELHLSRGYQLVYSPHIAKADLWKTSGHLDFYRENMYEQMKVEDETYQLRPMNCPFHISVYKDGYYSYRDLPLRLAELGTVYRYERSGTMHGLFRVRGFTQDDAHIFCLPNQITSEIKGVLDLVEETLGTFGFHDYEVNLSTRPEKSVGEDDIWAMAEAALVEALGVKGWRYQVDAGGGAFYGPKIDIKIRDAIGRKWQCSTIQLDFNLPERFDMHYMDSSASKQRPIMIHRAIFGSLERFFGILIENYMGAFPLWLAPVQVRLLTVNDMVAEYARDIAAQMKRAGIRAEVLGEASIGKLIRWPAVRPRTSFKMVAPCCVGRRSETVRCRNQRGSQGRGRGPGLYQRHRHDASICQDRPPGPIRQICGVLKARFAAARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1H9</accession>
    <name>A0ABQ5S1H9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_006490</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="518"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="551"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="631"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="664"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="746"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1255"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1285"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1331"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1369"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1400"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1426"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1483"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="116"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="900"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="853"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="860"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI63506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1511" mass="164956" checksum="13D89E3B76D8DA7F" modified="2025-10-08" version="1">MEKHAVGAGQADTGEAGFKTSSPDASCPKRRMRRVDTAAVLGAVNADSGGIQELAFLQRLAERLQRVGLSFPGVEVRWQNLEAEVKVPSHGPRAYTLLSAFLSGLDTALVAPLKALVCRDGRAAGPQSRVVLNAGSGRLAPGRMCLLLGPPGAGKTTLLKVLAGQALPGPAAVAAAGVDPSKAAAGSGGGGGRETGGLRVRGRLLYNGLTPGPDFTVERSGSYISQHDLHIGEMTVEETLMFAAECLGPGLNKELHDLLLERETAAGIPPDPDLDRLWSTAYGPHGHSLMVELFARLLGIDHVMDTVVGNEMLKGISGGQKRRVTCGEMAVGFAQVMMLDEITNGLDSNSALAIIRSFRNICQYAQATMLVSLLQPSPEVFECFDDVMLLSGGRLVFLGPRTEVVPFFSQLGLEPPPSKTEADFLQEVTASPEYQSIYRVKGGLAETSGHRWLSPRQLRQEFDSSPLGQQLVEQLATPPYSHELQHMVLHKERYALSLARMWWVVAMRELTLFFRNKIFFVAGAVQIAFTAFLVSTTFIRLSKNTFNDANLHMSVMFFSLMTIFMGGFNFCPIYCQRLAVFYKQRDHNFYSASAYAMSCFLMRLPELLVQSSMWALMVYFSTGFVEDAGRFFIYWLNLLAGGFYSVAIFQLLGAVTRHEVIAQGLGAVMLMLSVLASGFPIARSSIPPWWIWFYWWNPMAWALRSMAINELTSSAWSKPATEFNRPDISIGQYALIARGFFTEWSWVWAGVGTVCGLSLVLLALQVVALTVMSAPSTYRPPVDDEEEEKAAELRLEDENDNVQVVEMEQQANGSTDGSKIRKEVGNGNSIVNGNNGNSHSHVRIYVPPRIHAAAPPQPPAAGVGAAANGQKANGDGAADPALHVPTHGSNLSFTPVVMAFRGINYFVPDPKGSGELQLLNGVSGLFLPGVLTSLMGASGAGKTTLMDVLAGRKTGGRQEGEQMVNGAPKRMASFARLMGYVEQFDAHNPQATVEEALLFSARLRVPPGVVSRSSIRSYVAEVMEVVELGPLAKHAVGAGGAVGGGLSTEARKRLTIACELVANPSIVFLDEPTTGLDARAAGMVMRAVRNTVATGRTVVCTIHQPNREIMDYFDELLLLKPGGRVIFSGLVGRPNQTELVTYLESIPGVPKYQPVFNPANYMLEVTSPAAERDMGVDFAELWEKSDLATAANARLQQHMEAGCDAITAATGASSDAATAAAMATPRRYAQPAVVQLYLLVQRFLISYWRNPAYNLLRLVVTVLLGLALGTLYWDRGMKRSTLLGVLDIMGALFTTSLFLAMTNCLVVMPVVMSERAVFYRERASGMYSSVVFAMAQGLAEFPYLFVQSVVYVVVVYCTTQYEFDSAKALWFWLFIWLNLLCFTYLGMGCMNLAPNMPACVAMSSFAVLLWNLFCGFLIYREDIRPWWLWAYYFNPAMYSIYGCIVTQLGDVTDEVIDVGGGKLMSVADFVETTFSYHYDMRGWLVLIMIGFIVLFRSFSYIGLSFLNFQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1T1</accession>
    <name>A0ABQ5S1T1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006926</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="261" mass="29193" checksum="4D496FDA015C74EE" modified="2025-10-08" version="1">MVVHKQEERRPPRAGLAYSSLALSYLTDRAQHHILPYQVVGKLGIVAPCPVHDSGADMATSDNTRSRVISSKTYAMGRTGLTLPWLEACQRFSSAGAVDGAKYLTSCLSKQLKDIWGLSQSQLELCQVTVRRICYLYYAPYETVVYVLSGRLAGTLFGNRTEAAVCDVSVRSDENGVWYGRVQLLLSMTHTQETHDLVYVRWFEAPEEDWNRELHLRPLQWHRKPGAPHASFGCIPLESSSSVFELFGPHGQLWGNVGPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S291</accession>
    <name>A0ABQ5S291_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006333</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09212">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036339">
        <property type="entry name" value="PUB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153:SF38">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153">
        <property type="entry name" value="UBX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143503">
        <property type="entry name" value="PUG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="643"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="695"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="695"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="765" mass="77616" checksum="6D0AA7F1D593AB04" modified="2025-10-08" version="1">MALDGLGEKLKSLFKPKEDPRFKGQGRRLGTAEAQSNFSSNTNHPQHGANGVLAARRPAAFDTGEGPLETHTPLKPPPQPQGPGGRPALVLSRSHRTEDVVLDAAVMANTPTGKEANLVAAHKPPSGISSNLGPAVPSPSHPTSSPGAASPCSPTTAGGGGGGNTMDTQGLEHDLQRSSGGGRGRHSVNSGGGQSLAAPGRGRTQSRAATSNLSSMVSTISTPTAAEKRMGGFRCPAPPTDGAMADYGLAPATSQAYELQRCLALVLSSQPLSNSNLSTGSSASALAAVIAGTSMPPLAPLLQTPTLVASTAAAAAAPCRSASSTEAAEVSAAPSDETAQASLKPVVSSSLSAKSLTPSFTSSEAAAGDVAAAVVAAPAAAAGASEPSGAGGGAAAAATCTSSSGHQGTAGPRECCDALLRVLGNVMTKPEDNKFRQIRLSNPRVQETIVRVPGAVEFLEAAGFQMHFSPDDGSETANHGYLVLPDEIPPTALAEPFRILSAVMAAHGFVAAVPKPASVAAGSAGQPSQAPASQQKQSAVSAGAAGAPSLSATAAASSVATSSAIERCTQVLLPAAPDTKVPDWFFERTGAEIREQWAAIIRKREEQERFMTRTMREVRMQQEAAAAAAAGGAMTGGDIHAAVRVRFPEGVCLQGQFGASEPVSRVFEWVSGSLRSPALTFELIKPDRRPLETSATATADKGAGKGATPARASAPRPGRLLTVRDADMMPSILLNLRPTGPEAATLVANNVPLLSDELLRKTKPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2Q5</accession>
    <name>A0ABQ5S2Q5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF261">
        <property type="entry name" value="ZINC FINGER CONTAINING PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="211"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="485" mass="53794" checksum="FD07EF6E83118A1B" modified="2025-10-08" version="1">MYESSSSRQLFEDHVASKIAQLTEELCFYRDISRAFATYIMDLHYRLVGVGKTLYDLTGQPVELDLPSSMKQLVLVMATEPCDEDQTLSPSIRSMADDFPEITASITKADNLSAMDTFQCSTTVETGHWYSNTPANCCSTITDAGEVEAAATASSASPGQELSLQVHHVGAPKDSTKPGLMTIVSAHEQQMLHHLMEEGRSQHWLIDPLEITLEAKLGSGEFGTTYLASWGEKTVTVKVVQPRVDSELKCFLREVQNMSSLNGPNLVGFLGACLSPPEHLWLVVEYMPGGTLQQWLYRPDTAIVPLVARLEKMRQVARGMCTLQFRSLEGVSVPILHRDLKPENVLLDVDGNAHIGDFGLSRELLAQSQAEVSRETGTYLYMAPEVMRGEEYDSSADVWSFGVMLHEVITRSRPYSYLEEISFSLSPAQIALAVSGEELLLPVPTGIPVELEELLAGVLNWSRWDRPDFYTIYTTLGEIIESLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2R5</accession>
    <name>A0ABQ5S2R5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007364</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="3">
        <location>
            <begin position="105"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="499" mass="56754" checksum="184340AE58701E3B" modified="2025-10-08" version="1">MTSNYFPPTKFAASLKEDGVPPPATGPSPTPFANATYRYVNAPSTTPDFNMPAADDPKFGPGPRVGAMPERRLSISSNYSRASEMIFGKDALLGPRRPRPHKIWKKKYIKLIVVGDSGLGKTTLIKSLISIPGERLQVHDGSYTPTEQFRRDPDSLCSTVSWRDEEDRVIWVYKIQDTPGYGDELDVFRNLKMVQDYIELQNRKWLEMEQSRDRKEDLAELEDPRVDLCIFCIPPHRHKARSFRFFCFSVALKTTRLRPIDLKYMFELGKHVPVVPVVTKADTMTIREANTYRTEVANRIANPMVPGIHDKINVFKFERDTLERAGVQDQATPHPPFLVVASNDISEELAASEPPLFWPERRYPWGTAEAFNKEHSDLLAVRALLMKEALEEISKTKRQRYEAWRRSTLGGLKLGRKITSALMWTIIPAIVCLQVGRAGVRVEDVKKFVSKNFNRVRRLRIQQRVDRPTSASVEIEEAPKSAAVPVLVPSPPPKKGWGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2R6</accession>
    <name>A0ABQ5S2R6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Intraflagellar transport protein 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007350</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028172">
        <property type="entry name" value="FT20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31978">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31978:SF1">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14931">
        <property type="entry name" value="IFT20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="62"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15547" checksum="D0C6E2D640F91607" modified="2025-10-08" version="1">MDAADRGVYFDEDFHVRILDVDKYNASKSLQDNTNVFISNIQNLQSLVEKYVSAIDQQVERLEAEKLKAIGLRNKVAALSEERKRKQKEQERLLAEKMEELERLQMEEQSLIKVKGDQEILIQKLTDSSSGAAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4C3</accession>
    <name>A0ABQ5S4C3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007469</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03752">
        <property type="entry name" value="proteasome_alpha_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1 2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="4">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="253" mass="27836" checksum="459F134F945AF048" modified="2025-10-08" version="1">MARRYDSRTTIFSPEGRLYQVEYAMEAISNAGASIGMLAKDGVVLVAEKKITSKLLDTHAVGVRREKMYRIDNHIACSVAGITADANILVNICRLAAQRYLFAYQEPMPVEQLVRNICDTKQGYTQFGGQRPFGVSILYAGWDEHYGFQLYQSDPSGNYGGWKAVAIGAGHQAANNLLKSDFKEDLPLSEALPFLIKVLAKSMDTSLSPDKVELATVTRDQDSGKVIYKIYEATELQPLLDAANAEKEAAAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4J5</accession>
    <name>A0ABQ5S4J5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051341">
        <property type="entry name" value="Zyg-11_UBL_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12904">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12904:SF23">
        <property type="entry name" value="PROTEIN ZER-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="869" mass="89483" checksum="009D106F7A60EC76" modified="2025-10-08" version="1">MSLPSLLDLCIQAAASSRHWTVQRRNLERLPDQAANKLLELLLDRQSRLQGGGRGGAGPLRPATMELFRCSATRVVLRGDAVTAEWLAALAGFRYLDTLHLTGCSKINAAALQALILSGSSLPSSTSPSTSPSASPNQPLKLGSGRGTSDASVHAPREASPPSRRVPVVASCAGTCLRHLDLSGSSHVRDDALPLLAHLTHLQSLNISETGVLGTGLSTLTALVGLTRLQAGGLKSVGDEAWVGLLSGLTALRHLEMWGSNAGGGANGGEQLLLLLAAGLPHLRHLNVAWASLTTLPALPELQVLDMRHCQLREVWWPAGHPSRMALRQLLLNGATLSGAAAVTDPESPMGLASLLRHSAATLEHLDLSQLGSPESWTAPLAALAAAADGIAPRLTHLDLSRTAVEPADLACLQCAPALQRLSASGTKAGSPQGAAALVAGGLAGLQDLDLSSCSLTDITVSWLQQLTGLTALNLSGNPNLSLAPTASLTSGQPPDAQQQRHLALEAMAFPDVWAASNAAGMLAAAAPPPLDPDDGARLAAVAAAAATSGSGMGTVAGGGGYGVGSNSSLTGQQLPGSSADCGWVWPYLRRLNLLGTRVGEAGFRTLFTTANECSSEGGGLEVSEATRAYKGPLRLLEQLKAGGPGLTDGAVGILAAARLPNLHSLLLRDAPLTGHGALLLPSSLPSLRRLELQACWLVSVQDAVQLAATCRAGALVVINGKAVANAGTSAGLTSGNRAVATSPGSLIRTMVGISTCEREQSAAGRGLNSAAAPRAQQNRGAASAWTIAAGIPNNQGNTVSEQDDLSHFDQRLRYDKKTLLRLAASPLIEGAGIRLDGRKCREIVAERANDGSEHLYLALADLPEAIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4J8</accession>
    <name>A0ABQ5S4J8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="135"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="398"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="831"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="898"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1095"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1147"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1262"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1416"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1535"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1626"/>
            <end position="1653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1670"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="834"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="857"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="915"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1095"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1163"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1267"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1416"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1434"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1557"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1571"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1634"/>
            <end position="1647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1867" mass="192440" checksum="D6FC4C2E7EAB232B" modified="2025-10-08" version="1">MKAHIQNGVEETSPSHSSPKRALETSTSCEAGGTTREIRLANVRAKVKALSSGRSYRAFDAITDLIAEARINKASLLDTRRGALELVKLLALPTGQRRATTYRDLCELGRNLHLPSWHGSERRHLYVWYHCLQRMRLVEVEAGNVLMKQGDVGDTAYWLLHGTMEEYRIGPPQSSSPPRSPVRMSLLRARSMTFDRLKSMGDGRATAAPAPAATVVAANSPRISSNGGGGGGGGGGGVENCSAPSPALVTAATATVTPAGLESPSRLKKHNIDGGSSGGGGGGGGGGGGVEVISPLPSGELPSLTSLPSVRSVAKKVKAFERLGQEALQGNAYTVNVEATSPCQLAALSRSDYQAVVALAARESFEGLRAMSSLLANKTSRHPKAIADLASLLSVTEMFRSMDQEFRNELCGLLEFLALPEGTVVFEEGDKIDFTYVILKGEVVLSRYNETRNISEEFSVKTTGQQFGTMSALHVSAAAAAAAAGASGGSAAAVAAAAAGGSSTITADKKRNFSARCTQLTEFAIVTKVGYTRLIRNQLQKSLNTRLELLQGMAIMSNTTTQSTLTRIAINCQDEVFSAGSTIVDPSCPPTRLFFIVEGQAKLLWLAESLGQLPPTYDRPLDAAGGGGGGSISISGSVAGGGGGVSADGSGMSSVMRDGRSIKRRQQLQVYELALLTRGDVVNGMVIHGRTSCFTVVAATNMKVMSLEVAGGLVALEQLFGREQAAMLRELVLLQAEVHRERAAHMVAIRQNAAQLKDHRQGGFGMPPHVSDSPATFLQHYVPKTPGVEAMPPTPLVGYAAAVADASLFVPGAVQHGDVMKLVTAAGQSVLDNPPSPPLPTITSPPPPWRMRTSLEGSGPSFSGSTVVGGGGNLSYSGSVPFGYQQTPKLISREFQQLRDPQPSNPRRPSPTSFQQQLQQQQQQLQPHHVTTVVAQPVAIPRTRMRVPSPGMARELGLEVPPPPPSQQASASALDRVSSLCLLPDPAGGKSRNGWPRAPHRTHLGLPFAGNVAMLVLENHSDLVKVVNSSPSTLDCVATLRTVTNMPSNVSYCDDDGQCEDGSYGSSSAELSYRSHDGVGHRVMVFAAQPVGPSAASTASSPPTAYTIASEPPTPQPEAYSMSTDNALGTGMLSSLTTPSVIGATETGFDAPVTHGSDLAAGTATSPIRDTSSGASGGDEGGRLSPFSSMPVALNAIGYDEYFDRSNGGASSDGADISSGNGGVSELSWRSRQPYGGLSSPLAHVEPLTLAASQSVLQMQNSMSSLGAGGGGGGGTPGGGSGVVSAATASPQPLMPTASGRSAAALLSTASLPLAAGQVHVPLASVYRRMAPAPGAAGTAGAMLTPVLAATAVVQPAVSAETAKLCASRLTTVMSTGPMAEAAEANAATLGGGTETSNAAAGAGAGGGGGARAVAASARLSSPSSLPPSTSSSYTRDYNNQRHGSPVVNFGGLTDMSPVRTPPVMATAGQDNVVILPRMVVYETALLDATAAAGSGSSPASPRGGVSGGGGPTVRLASLGPAAALAAAAASAAVTAGPGGTATSGHSPTRMLPALPGTATAAGGVTSSSPPGSPRLRPPPRMLRSMDAALPYHYGTSRELLGYFSSNPRLASESDDAGMVFTGGIGGRPVRYGSPGGQRRQGGGGSGNAAATRRIGGVSLRDLAGVNGIYRDETRSPMGRNAASQKGQEGPDLPLELDADAPSLQNRKLSEPSLDDLKYILTRWSKRWSQERSLMGCPAALGPGLEACTSVSEPGSPLRKTAPSDLLRYGYTSDDLYRMAKEQHQQQQLLQQQQQQPHHQDVVGVGAFKRPLAQLDLFGLPYIGGTPRVVDHRQEAISGGGGPACLRLNSATGALAQLAARSFKGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4N0</accession>
    <name>A0ABQ5S4N0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_008142</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22459">
        <property type="entry name" value="KH-I_PEPPER_rpt1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="59"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="338"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="428"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="672"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="551"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="757"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="805"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="901"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1117"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="575"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="620"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="764"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="824"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="901"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="938"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="964"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1049"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1117"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1168"/>
            <end position="1187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1234" mass="129253" checksum="CFB6C06AACDB409D" modified="2025-10-08" version="1">MGGDTKPQQAQKTQDAQKQAGEKEQGDGLNQGAGKRKPQDKDYKEKNKRRRADKPAAVKGIHFRLLCYEGQAGAVIGKGGETVRNIRKTSNAEVSVLPPIQGHRLRVIAIDSPPGNRAAMSYPAKEALLTCSTLTVEGDQPLTAPDPSKMVEVRLLVQSDLATGLLGLKHSLGSLTAGDISARCGANVIMPPDSDRPKLAESGEEYLLLRGPWPAVYAALGMVADEVRRQFFELREPLAEPPLGPPPAAFGFPGGPPMGPPLGPFDPLEAMQLGIGLGMEMAVAAGVAGPPLAGPVHGNAPLPGGGSGSGLSVPHGGDLSISGLIPEFRSSAEASTPVTVTFKLVVPENKAGRFIGKGGENLRDIRLALGLSTLKVHPAVKETAVRVLEVTSTEPAGSKRCAAVEALLYYVDRLGMSAPQNANANNRGRFTVKMLLQQQQCGPVVGPRAAVIRQVSALTNASCVVHKKGDMPPYGAPDEQELEAEGDPPNVMEAIRMVAAIIRGCELRRQGISVRETMGTPEFDLVRGSCGGGGGPHGPFAPMGPMMPVAPSDGVAMGHIGPAGASQPSMPPPSQQRQLQQIPQQQYPPQQQQRPQQQLHEERYIPTPTQPQGHVQASDPQPQLQQQQLPPHLQKPPLQKPPQLGAGTSAGQSTVGSQSGSVIAGLPPLGPNDVRLTIFVAAALVNAPEGINGTNVQQVQAVTNTVVTLRQPVVSGDYELDITGTIAQVMQAHTLLSNIMTIGAILQQKPQLAPQQAPTSAVGAVGGGAGGSGQPSAVTATAPVTTASGAQHQVVVQPNQGVVIATQPPPQQQQPAAIQSPVHPQQGAPQPTQQQQQSQQPVPQPAAQAQAAPTSAQAVPSQPPAAFSGAGQQIPQQQQQQQQRQQVPLQQQQAQVQQQVQQQRPLQQPSQGVPQQQQQGQQQRPQQYPYNSELAVTAQQEAPVQRQAQTQQQPYPAQPPAQSQPPAQQQQPAPQPAAVTSAPYQQQAQTQQQPSVSQASTYPQNAATPAATLQPQQYAPAPQSQAQQSVYSQQYGQPYTQEAQTQQHAYYGQQTSQAQQTLAQGANPFPTYGSAGYSSTNGIAQAPAAAPVQPGSQGAATTYDGQYAHAAATVQTQTPPTGAPAAGPAPGATSTTSPFGPQSQQQPYPGYGAAGYHTTPTASHAYYQQSSQAPPQQQPQQPGAPSAHDQYYMHMQQQYAAYQAAAVAAATASTPYPPAVYGYSYGTSSAPGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4W1</accession>
    <name>A0ABQ5S4W1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF53">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="114"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="345" mass="37382" checksum="06C9BE5125B20AE9" modified="2025-10-08" version="1">MSLRLPAGGQIRGQRGLQASQHHKLPHHFIDDYLLLHSSTRCADACAQASGNYNAEVDTGSHTHIDSLGGRPSAHSSGINFAMSGSSLDQTLSFPNSHGERLSAKFVDAGSKDIVIFCHGYASSKNGFLFPRLAEELASRGKSSLRFDFAGNGESEGTFSFGNYFREVEDLRAAVGFARDTLKLNVGAIVGHSKGANVVLLYASRYDDVPHVVSVAGRGIMSRGIKKRLGEDVLQRLDQEGVIEQEASSSSGRRIKYLLTKESVDERMKLDMLGEAAKIQAHVLILHGSSDNVIPVEDAHMLASRIPQHTLCVVEGADHNFRQPVVADMVIQRIAEHLASTVSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S583</accession>
    <name>A0ABQ5S583_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF31">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN CEP250 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="26"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="812"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="895"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="979"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1088"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1148"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1302"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="465"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="826"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="915"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="981"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="991"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1088"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1148"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1172"/>
            <end position="1181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1493" mass="152867" checksum="1D5752E83B41C21A" modified="2025-10-08" version="1">MPRWRLTPISGLALEITQDVCLNGSLRIGRNVVGPDALTLPPTMTQFSGMHCRVYANLSSGNMQWFVEDTSTNGTFVDGERVVRNSSVRLLEGSRLRLSSPPQETLEFEFVRDDVEDERGDNYSHYSRPQHQQHGLVHQHHVSQYTGPHHPPQYASAVPLPAAAGSGGILQVHAKAQQSHAAYGGVTGGGGGGSGMAAGNDTGGGEGTSVPSNPQVPLVNHKRKMGELADGGSDDTAVHVAAPGASIVAPNRSVARRINNNTPASGAGGGGVTMSYGMSPMGMAGGGRAAAAMAAARQSSAAVPSFYAGHGNMTAPLGSGTGGAGATAEALVHVQAQLRAEKNEKEVLKRQLEVLRRECDDASRNLAAVREQHARQLDRSANERQMSTSREESLRSMLAAAEARAELVSRQADTAAERARGELGEAETARRHAEREASDAKAQLAASREELERLNAELRTERSDAAAQRAAAAAARRETESVREALAAEQRDKKAEQERAERLQQQVEVEKASAAAARRMAEQHEVEVRAARTQAEDEQQGRRAASEEVLSLRSQMEEARRQLAEAHERLSGLQDELAARHHRLSALSKSHAQAKQLLSGLQQTVAALTHMHSGLSHQYAQAIAAGDAMPQAEGGGSGKVGGAGAGGAGEGPIGQGHGTACGQQPAGALPVGPQTDADCAEEETGALGLGLADNSEGMGAMMQQSQLGGGGMVPVSHMALGHPLGTLQLHLPSTVPGYGFNSGGPSPARHHPSAARPQGGGTGMLYGSADPKGLRHGATNLLPTPPNGVHGQVHCHGNAAVQNQTQQLHLMSQHHQQQASFLMMSQQPQQQQYGQGTQQHQQHTYTGIGSAHGGSGRGAVEAIKPGLLQAGLASQPLQRVGFMTPQLLPYADTAATARGGTQPGNNTLTPPQQPLPASQQLQQQPVGGLGSVMDGIEAGAGTGEGAFPSAHGGSQLLSGSLPHPSQQQQIAAAGVIARDMNGGGGHGGGAPSAASASQQTTPTAVMREGGAGEWQDDNLGVGPCGGGGHELDVMGGGSRGFEEDEEEGGGYTSHVRTADRKGSGGRRASLAAAGALAMDVDAAVPSCGVGGSGGDGGGDATPQGQAKQDHVSRQHHSQQRQFTTPMVDAVVPPQDELDVIASRGTPCQNVSVQPTVGETSIGPTAPPEATRSGGGAVPGGAGAARNAVGAWPRLFSRHLTPTKRPNTAIEAADVAADGDTSITDGDDGTPASRPRMFAATECNGGSAAAGAGAAAGTRICMAGKNNKGADATALVMASSMTSMGQGVREDRGRDHNQSQLMGFMHSAGDDGGANELGDGAEPRSQELSPQGRAGVCSAVAAPLVREWEQYGLMAQERAGGSLLRGTGSQQPPGLLLGFVEGQQDSLGQITLGGAMTGGCVGGSVRSGLPLPSVAATEEVGLVGTTVPVDKGFGMGQGVLGAGPGAELRPHASLLAPLPSSEEALEQGLGVGAGTRKCSHIYGLHNMAGNDMSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5F4</accession>
    <name>A0ABQ5S5F4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="132" mass="14370" checksum="05E503C7917322A2" modified="2025-10-08" version="1" fragment="single">MSATEIMSPPRNSSWEPRAPLPTSERTPENSAWPSTDGELLTLLETHGDLLVRVAAGSATEGLQHDLTATVLKLQVAQLAAMAQVHEIFQGLQREISDFQSEATAQSVAEHRLQMQAVVELARQHAQLSTCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S616</accession>
    <name>A0ABQ5S616_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="821"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1046"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1215" mass="127578" checksum="3B4D0DBB2BC505DC" modified="2025-10-08" version="1">MNNLCRCDVSKYQRQVTSTVKGASVQVCSLQRTRSLRGNSVAIGQRPVVCHAGQPQDPQRPDREEFELQEGTESMGHGLNPQPPSNARWEHDFHPEKLVVPINASSALSGMEIEAGDAQLARSPSPSSSPVQYSHYSTDPKGWYECHLFEVLQGAHIDAQAASHFFSHLAVHDSWASPLTMNELLHTVRIADITGFALPPVIVAQLQNLVQSSGPQASQSSADPLLAAYAYDFPALLDMLQGTEITPSAAWTDYALHAWFAAVGGRGGGQLSAAHIRSVMTILEAIYIPGAEPSVEPQSPLPSASSSQNRGPGAISALGSGASTTIVNSSLASVRGWLDACLTALLDSDALRHAHQREDLYGMLPHLATAPSAAIVERLYSEAWPPYNAPSGAASPEHIASMLWLLAASGAKLPPAVIQGELRALEEAVLRIAWNGSGDVGTQGSGSGVGGGPVSNSGGTADGADPTAAAAAALKAAFALPSSPGASGRASSPSPPSPSPLQFSAASDPLDSMNSLPLSSSPPPLQPQSLPSRPDGGRRISPFGAYLALWAFHHYHYIPHAASALFVGPTLLSAAVNSGGAGAGRGLGPRQRCRIVRAVLMCFGSVPPQLFAKYDWSSLFRGWELLGKRELLPLLQDAAALAHQRRKRRQHGSEGVQGDVGAQLLRLSHLVAHVVQLTGWQRRLEHVELSDAITSLAGLLAVLIDEVSDTAGREAASESGAPHDTKVAAEARNDIDGGGVAEGSFLYSSDGDGGGGGGAVSAKAILASPFAQGLLASWLGLVLPAIQDAIKHLTGDWGNAGRAAGGVGGDRAGGAAAAAAAAAAADRHKQHRDPYQQFQDPQSGRGLHDVGGGAVSGSMVRYSDPARPPRSVAISAAAADARRKHGTLAWRSPSGLPPPVTAETVLAQRRPPPLTAAELTWTLMVLRDAAAALDPRGHPALHRTLMWAMQRLGSRAGKCAVEPEAFAFVPRMLVQMDTLGWVDVPLEWAHAVVEYPDAEGPWFGRLPWHEVMHAVLGSAQLVQRARAEVLRCHGAEAPVAVMTAEASESQRSRQYQQPQQLPGDSPGPVLELDSDKGRNHVATMAAVSSGPENSRPSKAEALLAAAQRLRSHLIGLMSARLVACSVVELRQVALTWQVYGMAYDKEVLELMVSALAGALAAALVEGNRGNGASVTALTLGSVRDAAARLEMMCESQQVAQEEVEHLFRPLSVYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S638</accession>
    <name>A0ABQ5S638_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="655"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="785"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="906"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1003"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="797"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="848"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="934"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1208" mass="124824" checksum="1C2A61D54BB404E0" modified="2025-10-08" version="1" fragment="single">QPALKLFTGPLILAPPSARCATAAVSAPSPEEPPMTVEERTTKDARSPNEQDPHVVSGDCSLPLPQLETCRQVKAMLWLNPEDQVLADADVYDSDDDISDSSSLAGGRLCSFGGHGIGPYSCGGSGGSGSGTASSLRMTDTTCGSAPEASPPGIAHVSSVGPDTLQPSSALRGATSLVVNSAVVSYASPHPGDSSRMPSRQTRNCPVTAEAGQAREHTGSGGSGSSIAADCGGGGGGSSSSACGVSRVAPEGHAGTDAGCCASVLKQCVSDQDQLPPKRRCLEGGTAAPPSPIPSTALHEGGGTECRCACRKAPPSAREQLLQKQLPASPSSELEVPVGQGLATGSHDRHRDGMVVICRTSGAGAVQSGSPPSIGPRMWPEQGRPQPQLQLPPSRSADGGPVQLACQQEDHLARQPFSRGYIDPSCSTCNDFTAASYQVAAAPSWISHHPCVTSSLEHASAHHAQFQIPQPSLSSRVCGGGGTATVPALAPVAKVQQMPPVCLCPSCSPHSGMGTGCGSHLTATSCGTAVIPNTACLDTLGAFSRQEPAPSWASRHPLKDSTARVLAPATGPVSSVPVALAPPHVRAISPSTSTCPNGHWSGASSENPQSLACHCSLCAIKPRPLLSQRPPVSSYTPYHGVRVTQPFIDPNKSYGSGGAHTISGPTPCLPGRHAPEQSTGPVGQLSHYVPPDSRPMAHQPPRLFRLATSPGGPLHASQSPLECAPCGQKWMNIANGTPGPGRGHSHQLHHLPCSIKRELSLPEMPSVTSDGWADGGWKQSLHLRCEERSPSSGGAAVPQRQSSGVSSVGGAPVSRRQVHEPGPSCSSDATMGRGSAEVPSEAQRHQKQPLPGAEPAAEQQQQKKKGRISSGSSAYSDPAPESVSAPNLAAAAVGEQARVLPAPLHEAEREPPEAPAAAPNKGTGAASNEPQPPQQLLQQQQQLLQHQRQSQRQDQLQRQPSLQPPQVRLNTNSSERPYTCWEPQPYPWHVAQDRVGVSMELAPRQQRRGAHAGSGGAAHNALQAASLSRQARSDRNAVALATTNFPPQRDSPHHYVRRCLLPCTPLPSAVAAAAMVPVSSCRVAVAAGGCGANLSAAPGLPPAADSGRSSRVPATFEPTSSSSSVLIIEGSTAAQMTAALFTCRCPHCRSPADPVMQGPSWQTVARHPDVMKAPQDAAHYTIRSPQQAGAAPVAADRTHWSGVVLANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6X4</accession>
    <name>A0ABQ5S6X4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13361">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13361:SF1">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="997"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1000"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1137" mass="120059" checksum="FE4303E6A69F9EC0" modified="2025-10-08" version="1" fragment="single">MLLLMIYQSCECYIDEVPNVLRRGCSQKQLSTFQLVSLALLLLSCSALQTSSAAPCPSSLHGTYDAVLLSAHEIVTLKSLSRKTAAAAAASLQSATTATTGPAGSSLGDGSSGNSGSSGNSGNTKPDFSGDGSRSSSAKDQEPLSSKGLFLRLRPTFTRQGCDATSGTSHYSLRLKVLDCDAFRGRVTPLRPEACTQSDGCTRIPLQCPRNCLHDLLEATEATFDVDMEGVVTSLDLGVAVDGTAVPDDDDVKENTEVKRKDRNGISENKDGKSGENGLLEMARTCSSDPWVSSGAKQMQQKQNIQQGSGADDIRDAKLRVESLRGIFRLLSEQAHNRYNNVSEELPVATTLSYGHFDNDDDVTEAEATNEGAIGGSAAGRQQHITTRSLYAFNSETTVHPSDPDGCQLVTQRKFLVPSDEAYDSMSNGITDFPAGGMSHTRLDPTGVVTSRVDMALSERTIPVRKQTSRSTSRAGGDGAAADGATILRTRVVLTLLKSSPENPPSSGSDADAAAIAAGAADAANAAAARIDGSERQRQRRVTLSGFEAVAAGPELLAMNADSLYPGGSFTTYLASWKDNLAVNSQRRRRRVLQQRKEKELHERQRQKQHLFGGNNDVGGAVGTATLGNEAAASTRYGSDAYGGDDGGGGIGFSLSAPAPLVKGAARSRRLGPLPSAAAAAVETGNTATMVTAVAAKGARRRLIDLNRTEVYRGFRSMFINGNELGVELAAYRSAGVERGYRVARVKGGMDMNFSINLFGNWYSEVSYSDAFGIAEFMRMNNGTYYNTAGILTRQLTMSSEIKLANDSETYYNPRPNCSDFESYSQPGFKLISAANKTLQAVQVKFSVISATIALQSTTRLYGGLGECNYNGWASIAVGGGMSGSFVGIASQGLDLALQEVQVNAEIYICQPDITGVVLYTLNMAMYDTTTAPSCTFADINLQLLSVQIYYKSMTAYGNWSEDKTVFENNGFTVATTKTGWKCETAAMTAADVRALADVTPPPPPPSPLPPSPFPAPPDPPSPLPPQPPSPLPPSPLPPSPFPPNPPPPPPPLPPSPQPPPPSPPPPLPPSPHPPLPPSPVPPKPPSPSPPPPSPFPPSPPSPMPPLPPPPPPPSPPPPSPPPPAPPPPPLPPSPYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6Z4</accession>
    <name>A0ABQ5S6Z4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4203 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24434" checksum="35914DAFAE81BBAC" modified="2025-10-08" version="1">MCTSATHKKVKMTMAHIVRLDNCSRSVIKKVLDEVNFPMVGTVLAGCVLVLCANWTSKSMAIRVTGGGLVVGLMMGMIVFCWLRITLCLQGCSVLGAAVGMSAWLAGFWWLPSLTFLITNTYLVAWVGFFWFIGLVSVYMGGAGMNNPRLETLLALSFRLLGHVLLYIRLWKCCTSRSRLLISICTSGDFRHRCASTCKYSKASESKQRQRKTSRRREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7J9</accession>
    <name>A0ABQ5S7J9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009544</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16307" checksum="82C9C58F5FFB111B" modified="2025-10-08" version="1">MSRPRGMDCVGMDTFRGTTGLNSTTSSRASLYSKNGGNVSLDNTAVVLRVESPISGDLLIAPVAKNRSGVRDPVSGTPMRYEGDEAIVRTPATANQFMRTRKFYIDKDVDITIQTLRSTTKVNRNTNEKVGTAGTPTGMDPALLFYQGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7R2</accession>
    <name>A0ABQ5S7R2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PHD and RING finger domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009442</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15545">
        <property type="entry name" value="PHD_BAZ2A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16574">
        <property type="entry name" value="RING-HC_Topors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058746">
        <property type="entry name" value="Znf_RING-type_Topors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47177">
        <property type="entry name" value="F18C1.6 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47177:SF3">
        <property type="entry name" value="F18C1.6 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="5"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="154"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="55"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="281"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="345"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="38020" checksum="C3242F64624838C2" modified="2025-10-08" version="1" fragment="single">MRVECCVCYEKTRDLGELDSCFHRFCFECISRWAETENRCPMCKERFIVIKRKRLPPDLPGNSAPQGVGGSGGGGGSTSASGSRRATGLKRSRDEAAATDGAHTNDVSDDDDEDPDAGDGPRKRLKGTVVETRIVPDKKQTWQPAELLALNLESLRCQVCNRGDDDEQLLLCDGCDRGFHTYCVEMDDIPQGEWYCLDCEQQRALLADTARGRRRAGARGGGRGRGVAAAAAATGFRRGWAARGRGHNGAATATAAIAEESNSDSDSYVEVSADVTAEAAAGAGHGGGRGGDSDVSGYETIGSDAEDQEGPEVGWVRGSDDDEGGAEADYDHDDGNENGIGSSGQDDEDQEEDPGQQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8T2</accession>
    <name>A0ABQ5S8T2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NYN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="2">
        <location>
            <begin position="89"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="469" mass="48388" checksum="54B026DE46727A2C" modified="2025-10-08" version="1">MHRPPCLRPWKQSWPRVARRVPRDVPSFTIAPQFRSHGDPDQPKGPPGAPGIHNAGNATAATAGATSRLASLQPPGSGAALQNLLCNRRTAIFWDLDNVNFFSPSYSAPLQVRRLRALVDEAGAQLVVCRAYGNTDTVRRLTGILPLLERSGLMSVAAVAVRRDAADLRLVNDAMEFTAATATTLTHGLEPAVLCVSQDTDFAEPLRYLSERGVRTVAVSPHDPRRRTHAAMEPGTYFRRLPLPNACWAALKWDPVVTPVTDLEAFWLGEVIGSIPPQSSFDSGKGAGLNVPVKAAAAPTRGAAAAAAGLQRRRLSETAMVTVAAAGVAARATPARAPPSLSSSSSSAAAAAAAAAAAAAAGPMAAMAAAVTEEQAVEGEIGSTTPAAKAAGPSAEAKMMDVNATSGGEDKAVLCDDSYGNERVGVGGSCGGGAGAVAVTGMAAAVHPGLVASCPGEPTWLWLNPRFYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9Q4</accession>
    <name>A0ABQ5S9Q4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_010609</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045158">
        <property type="entry name" value="KEA4/5/6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16254">
        <property type="entry name" value="POTASSIUM/PROTON ANTIPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16254:SF14">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046141880" description="Cation/H+ exchanger transmembrane domain-containing protein" evidence="11">
        <location>
            <begin position="25"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="610"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="634"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="693"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="12">
        <location>
            <begin position="211"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="12">
        <location>
            <begin position="602"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="111"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="534"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="747"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="557"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI66678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="767" mass="78392" checksum="D1D32837DBA4D051" modified="2025-10-08" version="1">MRVMHVALLVTFLWASCGQLGILAASNDDVAGPIVKLDELQANPKNTTGVDEGNESKSGTIAEMLDKALQAEFKKETSEDASGGSNFNATSKQDLANLVTVLRIPGKTGVVLPTTGADDSNSTESGGGNGSDAHGGEGHDVVSSGGDGASNSTKSDTGGDVAVGGVEVEPDVDRIIDSQDNEFVLSKPNESVASLTLDQQLIRDISVLLVAAALFGSLFEAVRQPVINGYLIGGAVVGPGGLNLINELVQVESLAQLGVQLLLFGLGLELSFTKLRAVWGVAVLGGTLQIVLLSLVCGLVAAAAGAPLAQGVFVGALLSMSSTAVVVKCLEVMRSTSSAYGQITTGTLLLQDCMVGVMFALLPLFNSGLTTGPPVLTAGQDVAEAKGRAVLLVLSSGMRLVLAVAAALLVAKTVLSPLLRLTQRQASRELVQLSLVAFCVGISSFSQLMGLSSELGAFVAGVMVNVALGPGSTHGSSSSSGADSANGPGGGVAAVVMHAMAPITGMVPISGSGGSGVSPTGVVVTHGSVAISGDDGGPELRNVVPKAKASDSETEWMLEREGLLTTEDARERERERERERERERERERHRARDALGSHAAYYSIESARNVLVALFMASIGLIMSPRFLMDHLGVLTVGVGLVVLVKGALVAAVVRGFSVGLRTSLAVGLSMAHVGEFAFVLLSHAVQSGLLPLQVYMLLLGVTALSLLTTPFIVLLAMHWVLPKDGGGGTGPTSSGVGPGLHVLVHSSGTSSGVAPTTGRRGAASAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9Z2</accession>
    <name>A0ABQ5S9Z2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial substrate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_010520</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF12">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="24"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="119"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="286"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI66599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="40845" checksum="948C0212AE816CD9" modified="2025-10-08" version="1">MRGGDTQGDRNPHELHQRRRRELPTAARELLAGFAAGAANVTSGYPFDTVKVRLQSAAPGQYRGAMHCLCTIVQQEGVRRGLFRGLSSPLVGGTAETGINYLVYSRVLDALRPDHSSAIPLTSVAVAGAVAGVALSFVLGPTELIKCRMQQAGSAARYPDGPLQCLREIVATEGGLRGLSRGLGATMAREVPGNALFFAVYEALRRGLFGAQQQQQTTLALGPGAGEPLTPRPVPGLTDLAVQVDAPPYGSDVDSGKCRVNSGTETSRPGPLKQHGDDATRYNWVTEAAIAVFCGGTAGTLMWAAVLPIDVAKTRLQTARPGSKWDVRLWSHWVMLWREGGLASLYAGLTPTLVRAFPANACQWLAWEVVMRGLHDRGERVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA68</accession>
    <name>A0ABQ5SA68_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CASTOR/POLLUX/SYM8 ion channel conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_010703</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044849">
        <property type="entry name" value="CASTOR/POLLUX/SYM8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010420">
        <property type="entry name" value="CASTOR/POLLUX/SYM8_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31563">
        <property type="entry name" value="ION CHANNEL POLLUX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31563:SF10">
        <property type="entry name" value="ION CHANNEL POLLUX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06241">
        <property type="entry name" value="Castor_Poll_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="CASTOR/POLLUX/SYM8 ion channel conserved" evidence="9">
        <location>
            <begin position="277"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="346"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="592"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="764"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="862"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="973"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1026"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1070"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1126"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="357"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="595"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="608"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="670"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="773"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="795"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="817"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="913"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="926"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="976"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1030"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1095"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1132"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1172"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1193"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1213"/>
            <end position="1249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI66751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1369" mass="139035" checksum="46BCA8AF536DC0AD" modified="2025-10-08" version="1">MPGTNMVRENNAVSFLVVNAAFFTGLFTFAIFLGIVSDEVKNTFRSIKTGDYPVRVRDHILVLNWSHHTIPLLRQYDLARRYGGDDAFFRRPVVLLSDNPKQDMDNEIAERLKSNSLDVITRSGSPAMLRDLTTVAAASAHTVIVLHPGGAASHASAEAAKASTAMALAALTAPEAAAKNALPMVGDGSGGGSSGGAGSELGLPTGILSAESRAQVLSALGLAPKPTHGSVGQRLPRVLFQMPDEVLLAQDPVTSFTKSSPANIRGNMQALSITDASVIDRFTCQAAVQPGMLRIWASVLQHGPHSIKALMVPVPSQLVGETFGQARIQYSDAVLIGIMRANGQNGSNGHNGGNSGSGSSVAAPSGSAVAPPPAGSGYSLHMDVREVDNWELAVSDVLVMFVPARVAGLPQASPATAALFPPAAAAAAERIKRHGGYKAPSKKVIMAGYQWQDVPDVAAAFLDSAPDNSQVTFILSAFPSPEAGDPELPGSSGSCRIRYVDTKGPMLSLRALQEAGIRTADAVVLRPPIQDMERWCAKKPVTARIREADAMVMAGLIQVQDAVIASGRTDPPHVVARLNRYGSTETALRYFEMLSPPPPQPPQPSQPSQPTQQQQQQLATKQQQQVEQPQQQQPTAPQPQQQQQRTSSGSKESAAQPKQQQAPPKEGLQPPQPPQPPQPPGKDLQEQEPPNRLNQPPWWRARLPFARRTTATELEFKPPLAEALAADVAASPISSSLHPEDVFNQLNDQQRSLRWPVFARHSQKHGGGWALLPSSSSPSSSPPSKRWVQELQLWPKQVQPHAKQQQPQQRIEPAQTPKEQQQQSQAMVASAAAAAAGVDGGTFGVGGSAAAGGVATAVAAATPVPAPPSSSRDPAGNPKTQSSDIGHAGHHPVPGSGLDLSRHLPAMLHLPGAGAGGSGGGGGGSSSEPAVSSAAPTAPMPAGLAASAGTESSSSAGATRSTVAAAAAADVAGTESTGGGGGGGGGGTVASSPSGMGNGPLSLANSAAAAAQAAPFAGSKPSTVIVGRTSSASQQPQQAQPQSHLSLQNQSIKHLEGPLAATQAVMMGTSPPALPIGNEANAGGQLKGPVPGLSSGTGGGGGGGATATSLNASVSQAVAAMSSVPADGAGAGSSNNSSGATATAPAPVGSQSSAAVAAAAHGSSNSSSSSSGNGGGSSGSSLSGGSGTGGSQASGSGAAAASTASTGGGSSGPSGNNNNSNNSSSSSSGTNCSSSSSTAGTGSNATTTAVHDKRPIIRNPEIFLADDLIGALLTQVAAQPNYTQLVSQLITKDGAELYLRSPAVFNIASGERLSFAELSESTRLLRQVALGVVRPDGRCCLVPKPHELLDFGPGDQVLVLSDDYGIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAJ4</accession>
    <name>A0ABQ5SAJ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="568"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="602"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="808"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="836"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="660"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="758"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="861" mass="94291" checksum="94CB5B4EBC59A45D" modified="2025-10-08" version="1">MPQKVHPSSMSDNYDLSKPSTLRGKACLRSGTAGGDLVGATMDGKSVEWSQRSEFELLLDFLDTDGNGKVSALEVADFVDSLLGTQDHKRPKGSIVFEAVLTAALRHSSTDTDGISATDKSIRPPPPPPPVIPSPPPPPPGLKTDSGKWTSSRSEVEDASTIAPAPKHRPSQSQLQQLESATTGKLESIFGEVSIKELHHYGSTRFLTMEAVRKCFADLLADTDLWILLSRNCSVMTTTLFRQLLMYSADRLERKFSMMVFLKTYAAFNALPLWLPFRMLWCTRKNGTLFLVYLHQLTKLQNQLGPWISSRLPLHGTLVVLTMIQFIATYVPLVAFVAAHGIRAAFSAKSIVHFMHANPMPDQVSLFSSILPFAVLQFLTSSAALKLSCRDFMSLKEQCKAIFMLGDVASEFEGYQGLTVRQVMDNIKTKVELSMAKSSYRRLNALMPLIFSLSLETFRRLVRLFLVKNLVPREFLGKVLAPFEVYALVYNIWFAYSESADCAAYLYKLRKMSSIFKTLLSKEEALSQSLPWLNNHSPRNLLLWSRLRRYYQSPNCLELRWIEMHVSLVVLAAIAVTIVLIVTFVQKTLLRFTPENADLQLFVVWVIPPFIYLLSAVVLDVIMSASTGTEYHDAIMTLSAEAVFIADRLNCAEEIVHRYGDDDDEEGENGSGGCAAADSDEERLPSLLAGGGPMMRTTSTGAGSRKPASAVARQPPSETGLVRRLTGSTSGTGIGDERNSGSDPSGGGGGGGGGGGRRSSSTNNDRRGSGSLEGISGGGDGGGGGWLPAAHYRLSARERSRLRQCQQVIKSLVTYIQFNAEFITIFGVPIDTHLRNTLFSVSVTLVGAGISAFLAIVVKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAY4</accession>
    <name>A0ABQ5SAY4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044295">
        <property type="entry name" value="BIM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46412">
        <property type="entry name" value="BES1-INTERACTING MYC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46412:SF3">
        <property type="entry name" value="TRANSCRIPTION FACTOR BIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="137"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="402"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="560" mass="57411" checksum="EFC771D9584CBB7B" modified="2025-10-08" version="1">MQPTSLTGRQQQLGLLMGRAQPDMIEAARVAAILNGQSGSFSLQSGLGGAGLGSGVLAGLGSTPGGLNLNLGSLQQASQQAPQNSQMALPDQLALLSQFPHALLPPQQYSSTDRDLQLTGLRNVGKTRSSDSRSSSAYASRHQAAEQRRRTRINERLELLRKLVPHAERANTACFLEEVIKYIESLKRRTLELEAMLEAATGKPVAKSLGTQGVMGGAGSAGALGTLESDSQAPSPRQVTPPSQQLSGPHPPSGSGVGGTATAAASTGALSLPAPSSLPSQQSSQQQQQQQQAASQQQALVEALAAAPQVATASHHQQSPQLQLASVVSSQLAHLIPAMGGSQGQQGGLGTLFPHSGGALSISGGQLSMGGSAGGTSGAGALSLAQLSAGLQGHHHLGGTASLLPSSQSQLTSSSSVTQQQLNDLQTMQVMQSLQHQRVAAAAAAAAAASHHPLHAAHAAHAAAGPHHPAFHPINNKAFLHFNEDLFGGLKPEQLIPTRSLLGPMTSSTPSTSLQLTSAHLPTDSNTLLQVETARKTLSGSPVSSEESGVPLKKRKVLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB31</accession>
    <name>A0ABQ5SB31_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="159" mass="17927" checksum="F858F639B82B7E3A" modified="2025-10-08" version="1">MMVLATNAARIHQKEEIQAARRRQAKSPQPSKARNSSRNATNSSKAAAKKVTNDGDVRPSTKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRRKKVKSASEDVRCTRARRQRGGSGSKKKQELFEEEVSKDERSEDEQDDATSAREDDDNDDSEIEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBS2</accession>
    <name>A0ABQ5SBS2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011547</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057546">
        <property type="entry name" value="HEAT_GCN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044972">
        <property type="entry name" value="Mot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022707">
        <property type="entry name" value="Mot1_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498:SF1">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12054">
        <property type="entry name" value="DUF3535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23271">
        <property type="entry name" value="HEAT_GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="5">
        <location>
            <begin position="497"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="1365"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="1781"/>
            <end position="1945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="226"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="739"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1084"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1766"/>
            <end position="1797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2044"/>
            <end position="2070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="744"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1085"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1777"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="2045"/>
            <end position="2064"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2070" mass="216905" checksum="94A562FC327EB8A2" modified="2025-10-08" version="1">MSAKPQSSSRLQKLLNLLDTGTSDATRKAAAKQLSEIARAHPDQLLSTVQKVHVYLYNKSWETRVAAGEALAFLADIFEHHTPDDLRWQALACGVSEDALNVAEGPQTPLSFANFSLQQVIERGTPLGASGGQEFDLVLEPGLTPKARLAAQREVLKKRLGLDRDMGLDADALFNDEDLEAAEEVCSGDTTTRYSQPHARGAATCTGGSQKAQQVAAQELLRSMDVEKLSARERNRLKRKAKAMGRSDSLRPGEPTAATGQHQPGAAPGVGRHKSPRDGDGSLGGSNGDDGAAVGLGGSAAAAALAAVEVGDDEVQQVSEGGWPFQRLGDQVVVDSLDPVWEVRHGAVLALRELLRGHAAVAGVEAPLDGVPSGWAVPGGQGKRNLGAVSAAQVAAARAANQRWLEDCVVHLLCVLALDRFGDYGSDQVTAPVRETAAQALGMALTTLDAISVRTVAAMLRELQGQNDWRVRYGGFCGLKYMLAARMDLAPDLLPEALPLLRRGLADPDDDVRAACADALVPVAGALGVVGTQAVAELRLQLWDLLPQLSDLSAATNSVMSLLAHLYGRGPGAAAATEELTALVPRLFPFVRHTLSTVRASVMQCLERMLAPPVPTGAVEEAVDTAGAVAVPLPPPVWLQPLLPALVQLVFQNVMMEGEQRVLDASVRVWRLLVRCAAPATLCEAMAPEQFRALMALGSTQVGRALDPNLVLVPYAGEIGRLVPLALVRQLLGMAPGTVDPDNTATASASSSPTFRSPKRSRSSSKQQQAAAAAAAAAAAAAAAAVPAVPTDFIVGTQGEEGVARMRTIAASCFGTLLACAYASGSTAAAPELLATLSSPAATARQFAALAACAWLQYAKQRLRLLQPELLHQMQMHQQGMMLLPQPLLQQVFVALAATSCVTPLAPASLEPYAEVLPYYAQMRREVLALVAAAVDVQILLPLPQGVAVENVTTEHAVALVAAAAAAGPAEGSPLAQACSRLRGALASLQALEQYLHVTALAFMAAVAVHGGVLPDKFNIVIQPLMNCLRREPQLRLQEVCAEGLAELLALCSGRTPSPNDKLVRNLVAMATADLLVAAAAATGPAQQSSETPDPGASGQQSASRISRRGGEAALVAIAKRFGETIWQKLPTLWTLVAGQLGATSAAVRAAVTAGPSMAAPPLDGDTVAAVGSLHVLRVVGPALAQQLLPSVLPLLQDVCRWARHADPRVRQTAAKCVSTLSRGWTEGLMPELLKQVVPMLTSSEDGVRLGGVEVVAALCSELGPALVPYVVLLLVPLLRRMSDPHQDVRAAASTCFGSLMTLLPLAQGVPAPPGLDSDQLAAIHQDSAFLLQLLDNRNVAAYNLPFQLPYTLRPYQQDGINWLAFLRRFGLHGVLADDMGLGKTLQASCIMAAASLEAEAAYAGGMATGSNSNGADDSSPPLPLPCLVVCPATLVGHWVHEVETTIGPAGLRPLQYGGHPGERAASQAQFDKSLQRVNPSSPRLQYNLLVMSYESLRADIEWVSSRRWLYCVLDEGHAIRNPRSRVTQACKRVVAQHRLLLSGTPIQNDVLEMWSLFDFLMPGFLGQEKAFRAKFGKALQEARVSKRGSREAEAGLLALEGLHRSLLPFVLRRTKGQVLSDLPPKIITDIYCELSDLQARLYADFQQSKASAEAVEALRSGEARRAAAAGSDDTGDGGGAITCSSPHVFASLQYLRKLCSHPAMVMDLGLPAHVAVAAAVLRTTDAAGVDAALRRLKHAPKLTALRDLLATCAIISPTAEGEEEAAVGVGSGAGAGSSTSGDDAADSSGGDSGAGSGGSGHRMLVFAQHKALLDIVERDLMTPYGVSYLRLDGSVEASARFGIVQRFNTDPTIDVLLLTTGVGGVGLNLTAADTVVFLEHDWNPMKDMQAMDRAHRLGQRRTVNVYRILTRGTLEERVMGLQQFKMDVAAAVVNADNMSMDNMDTASLLDVFGAPGGGAVAGPGGSGGASTAAAAPGSMLPSEVEMAEAAVAEAAGEGGPGRKGKAQPKSGLAAALAAMGDMWDESQYSKEFSMEAFMHKIGGVAPGGVSGGGSGSGRSGGRGRGQKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCN8</accession>
    <name>A0ABQ5SCN8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="6">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011944</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF0">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="8">
        <location>
            <begin position="234"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="207"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="207"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="344" mass="36718" checksum="872C335057F1F871" modified="2025-10-08" version="1">MHLLDFIFGRRSVAFHPHGSLQTSRGEADASTCSPSSPLAASTVLQSSNLPPYIQLYQPRESAVVHTLGPRTSLSGQAGAELLVQFEAPSSSIHPELRTAAKGEPLVCKPDNVIATVASDISSNTEPAADPEAILKGPSLSLKDCHSRACAGVVDSFRKAMWPGGKPEQGHTVVPSNPSLHHSGESDIAVMRGPQAEVAATTLIDLSTGSTATGGDTSGTAESGGGAGGDRSNCPPDTWQLGRATWTFLHSMAAHYPEHPSPRQQELMRGIMEGLAEFYPCEVCAEHLREQMKRTSPQVATARDLNLWLCGIHNEVNEMLGKPLFNCNRLLERWRDGPPDGSCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD09</accession>
    <name>A0ABQ5SD09_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012099</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45617:SF180">
        <property type="entry name" value="ELRR (EXTRACELLULAR LEUCINE-RICH REPEAT) ONLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45617">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="5">
        <location>
            <begin position="123"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="405"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="756"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="877"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="909"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1119"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1271"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1311"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1402"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1550"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1618"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1670"/>
            <end position="1745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1993"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="883"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="920"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="931"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="981"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1011"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1125"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1180"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1196"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1240"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1311"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1339"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1406"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1550"/>
            <end position="1559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1633"/>
            <end position="1650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1675"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1716"/>
            <end position="1725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1993"/>
            <end position="2024"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI67818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2321" mass="237535" checksum="5B82F9A61365A1E7" modified="2025-10-08" version="1" fragment="single">AGAGGGAKAGAAAIGMGVLPAAAAEEGYLKLLPALAALRTQVVVIRQGQSFSGRELLCQEEAGLVQDVGGHLDLPGRAVAAVVPKESLVALVFVSREVYHRAMAMQSEISVRRAVDSCCRLPFLRHTPLKELYRLSRYLQEVTFQPGEVVLNQGRENGFIYFLVSGEVQVVLDLPDTSEPLALAAAMAALSGCKLTPQQAQALAAHAAAAPAATGHGAGRLARDSIIHTTVTADGTAPPNGGSAPAALALAAVAAAVGGPSGSSGSQSLPSFGVTRRIVLGRRGSGVVLGDDLLREKRLTVGVVATSRCVCLAVSPQRFTMCCDPLTLRLFHKLRVQHSATALFTDSLVQQEDRAALGAMFREAVLTQSYGPASLKRLEAPEPKAWKAPNVRPEEIAIPADQVFQQRPAHKTVTNASPSSNGGPASSSLAVTTTAHSGGGAGGSRVSGGGSTGSGLGTFSAAQTSHTAPVNGSTGSAGAGSLLPGAVAGVDGGPPRLYGGLTVSLVQGLVGRGAEVGSMATFLQRIKTSEADALALPSGSGGRDGQLGPRDLDMSQQMRGITAQPQKVYTDMHEAACMAVIKVELAPGQLDVRLDPLAQVLDDIVVQWSDLAHRWGLQLVRWRTHDYVLVTQLDTATSPGGKRSRVRIMADTLLSMAAEFRRIVAQNEIPHLSMCAGMAIGGLFSSYDSGHFLVHCSGQAYTAAQHLCRMACEESHWQGTLLVADSVAQALSRTHKVTFAAGGSVLVGAVGGPPGSPRALAHQPTGRSIATSVGLDGDEDDEPVSQGTRPYFKRGGDGGGGSRRLLLTSSMDSGRVAHFLRVDTEGGDDGDLYGGEEYGLGSGKAGRNLQHVLSSNHRTLPRMVSVTASLPHLYNQHSHHTLTPHSTGTQQQRSGRRYLSNSLSALASGTSNSVVENPGYGNGNGGGCGGSPKSPRGSPRGLRHTTTRLAPMGSPIDAADGGVEPSGRSDDPHHQKRQQTTRRPTGQSEPRVSFHSKQSLVRHSGDGGSGTNLRSSMSEASPVQLTMSRLEETGAGVGAEVGTTPAAASVAASTMWPHMDRTDKLCDNGSGMWGASIQDSQRSTGAGLRLTANAHHRERIAAGNAMSYRQQLLQEVTQLRHQRQNQEQSPSKPSPQLQQRQQSGPRRCRWRQGPPGVSDSGYLSDVEGSVGSGRSPWREAGGAAANGNGNDNGNGTSSARMNKSALRSLSPSAAVPTSTPALQLSVEHTPSPSPVSAGLQQQIEQQAEEQLQPQTQQQQQDQPVAGADVLTATTPPMDTASPDPSVVHVNVNDATQSPEPIELRQCSLSELSHPHSQSPVENQEVAQIPEPTSSPPRCPSLSPNPTRSSSSNVCLSPIMGLTSKPPHTPGRVRVVSPEKKQSPRTPSPGYVTAAVRAVEGADEVSGGSGGRGGGSSSGGSGSSAVAVATRDSGGVDKGGKSPTTQLAVGASDVVSIAAAAAAAAAAGAPLLTLPARPIGVPSRRAPLGTQRPTSPYIPFSAIDPTSDAAVVARGGSPPPHTGPPGTAGTVSYIPKLVACNYGYLQIPSKSRSRSRHGSAVRHDVVDGDGASPTGPEVTVLRNIGAGGSGSYSRSPSANAFVESAAWVLPFASRPTSVTLASGRGTSPVPLTAAGGGSGGSGGSGGGGSGSGVIPQIATDAADAGSWAGIRRLPGSSLETPSTPKTPSPGRGGGPTTINAALVSRQCSPPPPSAASGGSGGGGPASGGRSSPSRRRGGSWQDLGSQRAAEQYAASLPLGLMSVSFSTAGLSRVPPLQRMLYLESLALSYNHLVGLASEQVMGLSPNLKVLTLVSADLDELPPDLGTLLPGLVELNLCSNRLVSLPPTIGSMTRLRSLSVAHNQLNSLPTSLYTLATLEHLLLQHNRLTVIDEALGNLRQLQALDLGFNRITQLPNETTALGGCLTRLAMPYNQLEALPPGFLLSMTHLAVLALDFNPHLAADPQLGGGRGSSKTVNGAAPDAPADAVAAAAAATSDSLRSRSPSRLTMSSVASRAAASGAPGAPSTEQSMGTELSFAPLPDPTAPSVTTLSAPLPSGGSLQEGLSVSTSVIVASASALSHLLKASAAPGAAVPMPMSPLPPTPTSPAPGAAPTAPQMLPALRILSLSAVQLVQVPSWIPAGLQVLDLSRNNLRRMPAWLCRRLAPSLTVLRLHTNRLESLPSEIRTMSQLQLLSLEENPVSSPERIATPQGAGWAAEWLYRKKYRSRPEILASLVAEHTGLTGAISGLAGGASTATMGAVSTDSFAVTGGGAGGGGGSIAGGSKSGAALAAAASVGGSSGVVSQTRSLRRGVTGGSNTHAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDG0</accession>
    <name>A0ABQ5SDG0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin efflux carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_012303</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039305">
        <property type="entry name" value="PILS2/6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31419">
        <property type="entry name" value="PROTEIN PIN-LIKES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31419:SF1">
        <property type="entry name" value="PROTEIN PIN-LIKES 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="545"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="580"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="183"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="300"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="616" mass="64761" checksum="1D93947FD48EAA9C" modified="2025-10-08" version="1">MGEPLLLQLFVASSIPVVKILLICTIGAFCARKGLLTPEGRRVLGALSFQVFNPALIFVKLASTLTPSRLLHWWPLVLNTAISTAVGLGLGYLGVRLVRPPKQLRPHTVVSIALGNLGNLPLVIVSTLASSSAELLQGIAPSRAEDLAVSYVVVGLLVPVIAHATIGFNMLRKVDASELPEAEARAADGDADGQAETPRTAAAANAPAATPADGAKAREASQEANFLAKADAAATSPSLELQKQPLLEEHRITDSSSSCYSNASVLKRPNSGSFIRLYGCTVDSLGQDDVDPLLRPAAMPEKHYQQHHQQQQDFLNHSERSRHRGLSDPSGGELDLDVGAEAEARPVGVERSKGQPAAVAVELPLLPSRPRLTPEQQGSCGDALRSSAAGCALDRPLGCSGSGCRLLQCWRSRVDWRAVGRQVLREVISPPLLAILLSVPVGCVAQLQRAFFGGGSGAGAGSAEAGGRAPLGLLTDCLTMLGDCTIPSILLILGATLANGPGAARVPFRVITLVNVTRLALLPLLGLALVMGAYAARVFEAPDPIYLLVLLIQNTAPTAIMVHTMASVHANCAGEVSSILFWGYISGIAIIPLWLTLFLYVVKLQFRYGDGGALDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDV4</accession>
    <name>A0ABQ5SDV4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27536" checksum="5A774D385DA21B7D" modified="2025-10-08" version="1" fragment="single">MNRYWGPRPFSASRKAVTNSCRVVIITPLRRIRVYCNSVCSPRALLPASLSCSTRHAAATDAAADTNAANATTNRKPQRIVAEMRVDGVDVGTEEEGPEGAAYSDDDGPPPGEALVEDEELLQLSAAGGEPAAAAVASVSSVGMLPEADGEAADHPLRGLAWRFVITRQAYEAWGRLPDMWRRLVMSRLPIIGSGYWARCRGAKKLPADDPMLAQQELWRLKITKGGRILFEVAVEYDEETRTWAEMIRLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE69</accession>
    <name>A0ABQ5SE69_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_012293</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22583">
        <property type="entry name" value="Rcat_RBR_ARI7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="119"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="123"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="524"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI67959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="612" mass="69680" checksum="B917A71B0D9C6358" modified="2025-10-08" version="1">MSDDSDDFRSGSEMEEDSDAEYMEEDEDEDYGFEGAEELAPVPKVPYKVLPKEELDKERKRALSEVMSVLDVAEDVAMRVLRKYKWDVSRVQEEWFSKYEQVRESLGLVEEEPTPSGREEERCLICFDTYPLRDMRSAACKHYFCKECWRGYISNALSSGPACLDLRCPSTECTGKACVPSSLILELASPEDQAKYAAYMIRSYVEDNSAMSWCTGKNCENAIQCLIDRAPGEALDVICTCSATFCFNCKEEAHRPVSCDTVTKWLTKNSAESENMNWILANTKPCPKCSRPIEKNQGCMHMTCSQCRFEFCWLCQGDWKEHGERTGGFYACNRFETAKKRGDYDEESRRRENAKASLERYMHYFERFDAHSKAREKARSDARKASKDSLEKLADITKTPTSQLKFINEAWNQIVECRRQLKWTYAYGYYAFENADKDPVNARHKTFFEFLQGDAERSLERLHEAAEKELDAQKARTQQDGSFDIESFQNFRKNLIGLTDVTAGFFDKLVRQLEKGFDNMEADYAGQMDDSAGPGTSGDLGDKAGTSSGAGKGRRAKKARAEDEWEQLLQEQGFWTCVKCTRNNEDVAVKTCITCGAPRPGPGGFPGGNFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE98</accession>
    <name>A0ABQ5SE98_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012066</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="302"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="376"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="798"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="377"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="804"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1085" mass="114794" checksum="3B776D50FD817A32" modified="2025-10-08" version="1" fragment="single">LVGDEYGPSSDIWSLGCTVAELASGLPLFPGKSTGEQLWRIMRCFGPLPESQMAHLSSCRRLTRVRVPVRGRSIRDRLRYCDPDLVRLVESCLQLDPRQRPTAAELLHTPYLGGVSGMMVGTPLEQVYSGLLLTQQKQQGKSGQHERLARVSKLGCMPGTPPSVPTNASLWSDLPPELQGVVQQPGQLASYPSMLKARDYQQNKPATVFATYAYDMAELEEEKQEGTNQDSNSSMAPMSPEDIAGTQHVCLPAGLEDGGQQQEAALPQLRDPETKGCVPNTRGIIDNRSPGNHCAAVSNGVESVENGTARSQDPDSGGFSMPALVQQKGARGEASRAAANAAGAAAGKAPFHMSGPRPMQQINNTSVETVVDVAPASATSTPKLQTPQPSAPPARRNRSWRSSCSVDTATIAAVTPTGACAVDTNESTSTHVKASSRPMLAFKASDSLPGVVAAAQSALEDEHSTRSTISTGALLPAERRSLVSVSYQQRPPPHRFHSAVNDAPRVLERTDSSFQLSALTTMDPSGLLLTTIMNTGTLALCDTAVFDCCAHATADEVARVHAGPPAPNPCISALDNMAISTVDLLGYQPPCKTIPTFDLGNSNDEPYPSLLLSTQVQRDADGQGQDDNMLDSLVSTTLRHMPCSPTTTTSHRILVGQAAPKSERCGGIEFPTAVGASGFASNRRAKIASENISATAASSASLVDRGVLVVSTTMVSGSRDNPLAAVNPDALVAGIMANRSLDRQRTGIHGSFSPVAYHSNSATQHGGTRQFARQNHNSRLMVSAELHTDVVSKRLQHTAAGRYSRSQTALHLTETSRPVEPAGNSSGHILYAWEPAPSSTSAAKGRLEPAVGDSRTADSIVQQGIAQRSLATAVDPTDGSLSNEGVVSSRSRLRMLNSVSNGRITPTKSALVGIGAAATPSLPAATPGSRNRLSIMMSSVSLSRVGLETTTDTMPGSPIQAIRMTRDVGACEETVTAAPRLLPPLANPPCRSARNTRMQPLPQQQEVIGGALQYADSGMPDRDVSVYREGGIEFGSHVSTRLKQKGKPQLAARSSTGSMADVTKKGTLLKKWFKTVKGMFSTTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEW3</accession>
    <name>A0ABQ5SEW3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_012708</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI68380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="18960" checksum="2BF52D839B858F1F" modified="2025-10-08" version="1">MESTGQPGAIHASESAFSLLKSEAWEPTGGIEIKGKGLMQTFVWRPTMETPGTAAVRQLLPASSASAAAAAAAALKVQPNGVAGLPLSTDGGGGRSSALLRYTTTSSSSAVNAADGSRSRAASSVIAAAAAAATLAAATPATATTPDATSGANTIAADCQTVDGIHMKTTAAAADCCNEDSDPLVSMLLSHVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEW7</accession>
    <name>A0ABQ5SEW7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012889</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825:SF2">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE 3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="396" mass="43019" checksum="99D610A5FB914773" modified="2025-10-08" version="1">MRRGILLWNSLLSTMSNRPLLQSRTQSLSWCCPSSVSRKRLHTVAVAATVPGERRAKANVDWDSLGFGLKDVAETMYVSEWTPDKGWGQGKLVPYGPITIMPSAQVLNYGQGIFEGMKAQESAKGRVVVFRPDQNAARFKAGAARMSMPTVPEEQFVEAVKSTVAANLDYVPPIGKGSLYLRPLLLGTGPILGLGPAPSYTFTIFAAAVGAYFKGGQLTPIDLIVEERFHRAAPGGMGGTKAAGNYSPVLVTQLEAKKNGFSDVVYLDAKTDTYLEEVSSCNIFVVKGRTIKTPPLQGTILPGVTRRSIVELARSRGYEVREEPVSVHEAMEADELFTTGTAVVVSSVGSMTYRGQRRQYTEPGQAGKVALEMYSNLTDIQTERADDNFGWVVPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF58</accession>
    <name>A0ABQ5SF58_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012503</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12446">
        <property type="entry name" value="RRM_RBM25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036483">
        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052768">
        <property type="entry name" value="RBM25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034268">
        <property type="entry name" value="RBM25_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18806">
        <property type="entry name" value="RBM25 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18806:SF4">
        <property type="entry name" value="RNA-BINDING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00311">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101233">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51025">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="101"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="PWI" evidence="5">
        <location>
            <begin position="704"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="254"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="366"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="544"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="226"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="303"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="430"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="475"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="492"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="578"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="799" mass="86657" checksum="B900F79115216A68" modified="2025-10-08" version="1">MSGPPGFPFQGFRPNMPGGMGFPGPPPGFGGMPGGMGMRPMMMPPVPMGGMGMGGMPLGMGLPQMRPPMSAVPSMGPRPTLGSSAHAKGVYVPPSIAEKSTTVYVGKIASSVPDAVVRTLLDACGRVKSWKRQVDPDTKQPKGFGFCEFEDAEGVLRAMRLLNGLKLDGQELLLKGNAATQKYTEQYEQQKVAEKAGKKAKALQVKAAGEGALIPEGGEGTKGDEETAASEAVDEDDDAAADNRVLEIIMSVVSEREESRGPLPPPPPIPSGGVTEATQAANDFLAQLGSIGAHGKPASSSQQDRDKDTKEREAKERERELRDREREWAREADAERRKEDDRRRELDAKYEAKLREWERYERERAMLRDRERERRAQAEAEWRRAVREDREKYESDDDREMWERRPLSGTRKSAERRKRRLVEEDEDEVDRQREAREAERRAKRAVAMAGEASGHEGTPGPAGSVVYDAHPPPAQQQQGLQQQSYQQQPSEVKAEPKVEAKLEPEVDTNDPIYQAMLAAARAPPPATMPVHQTGAIKAEEVQRPLGSQREHGHGGGSEQARHGFHRRSESPPHAADGVGAGSSVGAGLTGGPAAATSQGAGGGGGVVSSSRVILGPGGRARANAALAAMFGDEEETTQKRQLKPIRYSEEEMLAVAEEQPTVAAIPVHTAPAPAVGPTIQQVQSPADSKAALKAMMDSIPTSREGVFSYEIKWSYYNASAMGEKFKGWVSSKVQQLLGVPEPTLVDYVVKLLGQHTTPCMLHAELSPVLDNDTETFVIKLYRMVIYETEKAALGLSQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG09</accession>
    <name>A0ABQ5SG09_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="605"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="663"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="609"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="664"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="683"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1268" mass="135175" checksum="3343A5DF1C18F800" modified="2025-10-08" version="1">MGRIPLLLPLNVKPSEATGKDLEITPRLQPVANLNSRRASYAYGASEAGSFNGTVHARQSRPSAASGSDVFGIALSFADRAVRPDAGLFPHRESRPGGSTPAVVSSSPLVSSDGSNPSRALYSIRHAPNFGYAGANSNSSPLPTATNSPIRPAPNPHAATTTWIGASISADPSSAVPRSPAVAASALPTGSATASSGQNLSGMTLAQFALSSCSTAEQQLRELLVERTSSVPASPAPIAAIKSAPLPTAAPVLAPTRSGPASPSAAVQCSPLPPTFRRAPGAMAAAVGPTVAAMASSPSTMPLHPSSPAAPSVQSDPHRLAVSACSSPITSPVPHLTVMSSPVSGCGGLLAALLDHRQMLAHSHPAASKPERVTRFAANADLGGAGGGVGGASAGQQHQEEGLEDGVVGGGDNDQESQPPPPSHAAALVDQLREKQGAEPQQQQQQHELEAFNSKRAGVIWASINPLSKRNTGDGNSQPHQQASPWGGNAPGPAITAAAMRAVVELKNMDEFSLVAAARQRQQQVAQRTSMTRHSTLKKLASVGKHSLRSNDSVKAYADGLGLGGMHGPQLPQLLATVRASCPEMGSTLEAEQLMATQGITLQSGGGAAAAEQGGTHTGSSSGSAAASPKVAPAAQPVIDASCDGGNSGSFLPAVMSPRLHYGSSSNRSMTLSTSGAAAATSRVHSSAPTGLTGTQHQTSEFAVNGDPGGALHAVAARPAAAEQQAEAAVRALAVRFGSSALPNPRVPWDQEEDELTVRERRKKLYEALQAANPAAYHEEAAEAAEAAYRALSERAQLKLQKLLQESTTEEAPVRQLEEALRELTSQRPFFLPPVIPKPKLERTFKEADITRCKESSWNVDESLFAQRKKESESHELYDTERVRLQQLNIDWKRVVSKARFRRMVSKGDLGVKEDGQSLEEELGEVREELQRQSDFIRAAFIYYSMTSPGAGATSAEFLQMSPSSWLAFCHDAGIVGKGCTSMDMQNIFVAVNFEEESETEESAANDDDGMVRFEFMEGLIRIAFVKYIHTKRMSDASDALGMLLEEVVASPSLPPEVRVNPNEWRRTRFYTPDVEEVLKEYWDLLISMFKLYKARDRARYLWPEHFMALLESNQLLGPSTGLTRREAKLIFAWSQSLVTDELRRRQRAVSLTLWDFIEALGRVADVISPPDPEDMEQFFLEEDKQTPEPDRLAYEYYRSVGEGGPLLRRPSAGLMSAPSRPLDQKLRQLLQYLVCTLREAWGGHDAREVAAKVMRTATFLSGGIEMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH12</accession>
    <name>A0ABQ5SH12_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021883">
        <property type="entry name" value="LPA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35498">
        <property type="entry name" value="PROTEIN LOW PSII ACCUMULATION 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35498:SF4">
        <property type="entry name" value="PROTEIN LOW PSII ACCUMULATION 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11998">
        <property type="entry name" value="DUF3493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="61"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="455" mass="50252" checksum="F3623642079E635A" modified="2025-10-08" version="1">MNSLGHPHSHGALAAKRLLQACRPCFIASRTAPRLPGLLVVARASKSTPTSKPQYQPTSAKDAIETATRAFKEQQDIDEAVRLYRTSLEMNPNEDEARAALYNLGCALAKQKKWAEASESIVKAINDYRLKLSVALKDDDLDQLRERREWIDALTRVKGGISRSTKVDLRTEAKAPFRLPRIILFGGLLGSAAIGLVIIVFRLVKSLQGGPGAPELNESLTNLGINSLAVAVLSYLLYRDVNQKQEAVRITTREELMGRLQVDLGNDRVLPLLKFRGQVRPVIVAGNRTFVEKAIREADSQYINLRDRAVSVIPVIFDSTPGEVDPEAKLRALKREFQKEGRGFDDAAAAAKKAAAEAAEAKARAKGTVMGGLMDADKRWRLEPYTVDEWKAWILEQKEFAGLPANEPNCYIQVQLDGTVRSSGVGTPPWRQLVEDLPLLSDIRTRLMDGVGPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIS1</accession>
    <name>A0ABQ5SIS1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44743:SF10">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44743">
        <property type="entry name" value="PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="235"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="316" mass="35174" checksum="D67AAB7BD6C09BFF" modified="2025-10-08" version="1">MQASFSGRSAPAAARGRSGSRSATVYVDCKQYDASVRVKGSVKRVFKYAADFEHLPEWYPGVNSVTRMRGEGINLKNTYTVERTIMKMKHESQYELIDVAPGRKVVYSAQSPVHTAVHQLYFMEDPTDRNYTNIRYIKKIQLRSIAGPLQPLVSGALSKIPEEGLSNLAKLLISPDTPLSKIQLDPDDEAEEIRASKPASGWSWQSMWDGLGLGGASSGTSVISARSSRVVDTLGYYAILGLDIRRVAKYSSDDIKAAYRARAMEMHPDKVSMDDSAAQQSATLKFQRLQKAYNVLRNPEHKRMYDSGNLVEELVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIS2</accession>
    <name>A0ABQ5SIS2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="292" mass="30918" checksum="7A81E25FA05E84FD" modified="2025-10-08" version="1" fragment="single">EERWTTERIGSYPSSGSDLVRAFPGLQGKLLEDLHSHAGASEPTFTSFSNVYSLTQLQKEVAEDASLWCDGTRPGAPEKTAVQSLTLTAGELVSLMQGKRGVVIVQLWNGFDRSRGQPVYDPWVIDLLEAALRTPGVRAMPSVAPGCVGLTAVLFADKAPWNVWGPHLASFGCQANTVAGSAYYKVLIGKILGYNDENIYGYARSVGGGLTPDVVSQVDADLKKLSKTKPKLPWNREGSRGKKATGNSAPAAAAGSSAVVKPPGGQRRGGGGNGPRSCWQRSPCLLPVAIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIU1</accession>
    <name>A0ABQ5SIU1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="169" mass="18268" checksum="0D7CC66D43D204C2" modified="2025-10-08" version="1">MRMLPWSYLSNSRSVPWLGSWVVQQVLEVPETTVVATSPSHRAAATAAIAAPAPNASKERPPRSGASPRQRVLRGAAAGGMWSGWTCATRAALICGSAVREDGETDGAETGKHPLWNVAQDKLFDRDWGIRQLLAILAGLSVADCGRFIAWDGRDLPWVTCLTRAKHTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIZ8</accession>
    <name>A0ABQ5SIZ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF1736 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014684</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052346">
        <property type="entry name" value="O-mannosyl-transferase_TMTC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013618">
        <property type="entry name" value="TMTC_DUF1736"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44227">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44227:SF3">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE TMTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08409">
        <property type="entry name" value="TMTC_DUF1736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1736" evidence="6">
        <location>
            <begin position="466"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="173"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="669" mass="72070" checksum="BF57DC709A67E138" modified="2025-10-08" version="1" fragment="single">MQLDRLGELVCAVVAVIVYLNSIPANFAFDDSFAVIYNGDVTSDSNPLWGLLVNDFWGQPISSAQSHKSWRPLTVLSFRLLRKAWTALPEPWRREVMARRSRTRLPYEEDEAARRGLDPLLFHISNVIWHALVSAMVCRLTYFLLLRRWRPGPLLVCSRRGLSRRRPWRHLERGMQQQRGAEQQQQQTHGSEPQFKSRVPYTGNEVSEAASDRAANAHEAASSGTGIRRRRRSAVPAAAAAAPSRSSGVASNRGNRKAVMTTTAAMEAARLFPQLHFPAWFSGLAFAVHPVHTEAVAGVVGHAELLCAALSLPAVMSYIHAAEAAAAARVAERWTRGAVAAHWVKIAVAAALALAAALAKEIGITVLGCMIAADVLLIPAVVTEWDDDHRTMRSDIRGGKGIAEAMAAAAPPHLSPSLLRRFLRLLFWAVVEEPKGMRMAAVAAVGLCYVRLRSWVAVDQLVRIHRKVENPIAFSTSPLERLLSTGHLHARYAGLLLLPIHLSADWSFDCVPMISRLTDPRNALTAALYSYLTVMLMAARPWGVVATWCRAAAAAAAAAVTGQERTQGKKRVEAAGGKPFAVAAGPAADVSGLLIRRRRGSKPGGDDITTSSGAAVSDNCANIDAATESAVPGAPPLRRLAAAVAAAAAVGGGVAEQLERQRPLKEVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJU4</accession>
    <name>A0ABQ5SJU4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_014603</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08530">
        <property type="entry name" value="STKc_CNK2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF6">
        <property type="entry name" value="SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="16"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="367"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="503" mass="54747" checksum="5C50DFB702C44647" modified="2025-10-08" version="1">MAAPSAYNGMPKFPNFKVHKLLGKGSYGKVYKVERESDNQLYALKEADLGSMSQAERADAVNEVRLLVSISHHNVIGYHEAFLLGNKLCTVMEYAPFGDLRYYISKGAKRKTPFPEEAIWRILLQLCRGLQALHSQNVIHRDIKPANIFLCANDLLKIGDLGIAKALTNMNFARTQIGTPCYMAPEVWSGRPYSYSSDMWSLGAVLYEMMTFKTPMEGRSIVELRNRIVGGRFTPISSGPYSQELISVCHSLLATEPSKRPSPATILASSPAGKWLHILPEPPVTHRPARRRWTSDGSSRGALLDTIVVPRDLKELPKRLPPPSYDSDNVVQPPAPLSQPTQIAVAPAAAMPQGAQRVVSWCSGVSVPNCKKQSSPAQASKPLLKVPSHQGPRPPSRENSRAPSPGEVRSILDNRRDNSRAPSPGEGRSILDNRRGFGGPAPLPPLFPRNSNVYGGAISSAAAAAAAAAAAAVAAYQQRWTPNPPPAAFNAPRSIPYRRMSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK22</accession>
    <name>A0ABQ5SK22_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015291</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02650">
        <property type="entry name" value="nuc_hydro_CaPnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304:SF4">
        <property type="entry name" value="URIDINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="4">
        <location>
            <begin position="141"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="427" mass="46587" checksum="6C4EA0FEE0B638B4" modified="2025-10-08" version="1">MLITCRRQPGVIRVKEALSQLLDVKVLNFAHFSHNNLWDIKLFTWQQFEMPRLDAIREGIGSGRNHGRHIATQIHIMLVWMPPQSTYPTYHLCISSTSARPTGSRLTSYGASHGSRAKDASQTADHRYRPWCRIYCQVGQEDDAMAILMAANNPEVQLLGITTVYGNVPTALATQNALRLLEMTGLSEGPQAVVVVQGAARSLRAGADVERIADFVHGADGFGDIGLPLPKSHHSRDHSAAEFIVRTVNAHPGQVTILALAPLTNIAQALMLDPQLGDKWAELVILGGAFFTGGNVNPSAEANIFGDPDAADLVLGSTSRLHILGLDVTQKCRLTGEQLQEWYDMDAIMLHDPTAMIAVLRPDLFEWRAGAVRVACEGVMKGQTVMDASVKGFHGHHGWADRPKVNVALGVQVDEVVRLICELLTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKS4</accession>
    <name>A0ABQ5SKS4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015101</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050735">
        <property type="entry name" value="Kininogen_Fetuin_HRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13814">
        <property type="entry name" value="FETUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13814:SF10">
        <property type="entry name" value="FETUIN-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="3">
        <location>
            <begin position="13"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="403"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="571"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="626"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="734"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="856"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="982"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1168"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1230"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1635"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="404"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="626"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="655"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="689"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="886"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="925"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="985"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="995"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1168"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1239"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1329"/>
            <end position="1339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1809" mass="184519" checksum="C82E4FCF2DE1E3B6" modified="2025-10-08" version="1">MSSMEAPYPAQLVGWVVWGKVKSLPWWPGQVMHPSKGPPEVRKMAAMKSNTGKLLVMFLGDNKYAFLPWESVVDFAENKERHSSTNYASKYWKLGLEEAEELLARRAGLLPATDHEPVDFATKKTNFTSLAKAWAVGSGSNAKQQNSSGASGGRMTERSKAIPLQPEASKFIASSTSSPDTKTIAPAPKVHHRSGGAGTDDGRPKSIGGVMLEWARYMPQVTATRKSSELSSAAEILRFARRLARGEEAVGDVAAANEHALQMLHSLALQRRLREVDGLVRQATTATAAAAPGATTAAAAAAATTTTTTLDSISPGYECIPPPPRAGDAGGSGGGAAGEESAKDDAAGVAGRPLPAPMGSAVQSDAGPMGLVPVVVQEMFTINNVAAVPVPARVLEALLKGGSDARGGGGVEAAAAPGGGTTNSTGGGGSHKQGVLAAAEAEAAEAAEAAGGGTPPTVAGVSPIGGALTPPPATGEAERPKADEARGEETTAALGSSPLVETAADGGQQQQQQQQRVTGSMSPEAAVRQVAMEVDVVLRVNGKLLPHLSIGLMMRRRTTASQNIGEAQGAGLGAVQQQQQQQQQPVSASRLDHQELQGQKALSQPQPPVEGAAAATAELVAVAPAEGANAGTPPPAAAAAATGSVEDSVDRMETAVAAPPAAAPVLPSDPASEFRQPQTHQHQHQQHQHQQEKEKEELEEVKTMEGASAGGTGVEESLRAVCKATATALSVDASASAAGGATQKPMSAAPPGTASPEPRVAPALLPPAREETANGHAGVPANIRAVAAAAKGDPIETASLDLSLCLAPWQRGEVRVTSWTVCGPRLILIAVSADRKRDGGLGGIKREARLLLAEAKERRAASPGGPDDDVVDNTGGEQQLKRRLRSATSPPAPAAAVAGAQTQAQAHGSTGGTGRKSSPLPLPPAATAAAAAAQVKQHQQAAGGKEAGGVPQISATGPAAAALMRSRLLQQKRLANAAADAAATAGGGGGGAGSGAQQPGSDASKSVSSAAGNGGGAGADARASKKVKLVRFPAPAAGANGAVPCSSSRVSSGGFTALAAGAAASTAMGTAGGVTGPSDAGVAPSAGPAAARRRVSYNNQLIPNPADDGVEDGHRFGARGVGCVPANVSYSCSTYDADWELSYPPWLPQPAQKGAFITVSHLLQNRNHQTHHYHHHHNHGAPQQRHKAEQSYGSYHLAQHQHGAAQQVAGACGSAAGSVMQTSAGVQFNHGSFRLPTTNHHHHHHHHHHHQQQHQQHHQQQHQQHHQQQHQQHHQQQHQQHHQQQHQQHHQQQHQQHHQQQQQYAQHNAHPVVSPRCNGSGAGGGGGPAARTASRRSSGYFSYSDKQEELPYPERQMDGWSSDAGTAMDVDYGMGMGMGMGTVTSAWPPGVVVYGAVPAATPGAVPPALGGASSVAECIGGAVTAGSGACEQILLDIRVGDEADAEGPADARPYGGGGDAAIAVTVHLQPRDSSTAVPSLVSPPQPRPFTGSGCDALTASTPLLPRPAQPSAAAAAAAYFNHPGASYLLPPITPFAEQQSIAPISPAVAVGHTRSFGPIAAAATTELSTTPFGASAAAAAAAATFGGMPPDDVDLAVTAAILADRHMYVCESAAQKTQDTTAPKGPQKLELEGTAAAAAGAAADGGGSAGEVPGSGLHHPSLSDGEVVRLRQECDSLREQVKLRLAPGTLDPLPPQQLPYAELVAQVSERILALALQLPTLSGLTKQSFIQFSRTLMQEAVVCGQAAAAVAATATGAIGPSAPQVSSGRSLAVARVAADLVRSAAAPGLPLHPEDRWAFINLAAELLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL03</accession>
    <name>A0ABQ5SL03_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PI3K/PI4K catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF124">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="2">
        <location>
            <begin position="1709"/>
            <end position="2022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="628"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="634"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="2022"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2022" mass="209651" checksum="68CB7E70F4592CE5" modified="2025-10-08" version="1" fragment="single">MRAETQREAFDVLQALVPFARLEDVISLLRNLLARGMAAVLSAPARLAFMQLCGRVWQHQGLTFSLEQRQHRAHGRVEGQAQAQAALIPLVLPVLAGALGDTDPRVSVAARAFWHDLLPAAPVERLVALLRLASHPHLSSSCNTAAPGPVVSESASRLLARWPNVACALLLQPSLMLAANDQPLYTSQPYECTFRDYTPSLGLYGGGGTLIGSRGTAAAAALRTPAFLLTQDMYGGGSMASLARRHPQGLIRATATAHFVPTMSLSDIGDVFAPAAAASGGSGSGSGSGSGSTPGASTLMQSLPSASMAESSATQRYGSSGARTVRLAARSQQISSRYARNAAIRGMQQREMSMRAIRRNGRIARVQLFRSYRTGELPDTLSVRVLSFLRPLAGVVDTDGPAAIAALAALAAAAAAAAVGGGPEAPTQPPLRALHSALVAAFAARPAAPSYVTALQAIATRFIDADDITMAPLLLPTADLTAAAAVNSGYEATAVLLEALQSVQEKGLEAQNAAAAAAGGSDVYDKSGPIAAAAAKRAANTLSTTLEALAHVYVELDEQDVVLAVRRRLLQCPGTFAMLVAEAAGRPRLAVRRAGQMSAALDVLQELGPCGAKQPVSERPQDQGLALGSSALRPQQPGGEEEAGGAAPTASVDVPTVATLLGPAVMTRGSAVAHVVSPVEEALWQETVASCADALGDWSRLAVELEGPLTDLGVQLPEGGGGGGGRLFASAAAVRTVAENLPRLLLPRGSTPADVSACGSSSSSTGAAVLLRHVLRSALFQLSEEDFQMAAVFQGGGGADVGSGSAAAAAEAGPSEGPALLILQLLQQLNDGGGGGGGGREAGVYAASSAPLELVAAEVYRACWRGSWERCLSVLQAALGRLRARWSGLHPMSSAARISTLTLLQPLAEIQEMVRQLQFSRCGGMAGAGGGSGASRVTEPPAASAVHHLKALVNCWQRRGMGGAVASASASGSGAGARGRNPTGTGSALNTLLQVRRLLVDAATCILPRVVRGGAAADSPVSYRELQCAARVTHFAMRFTAAEAAVHAGCADIAAEALPPPYQPLPEDDIRTAAGAAAARGPEADVRYAARQLRLFRTHADTIAAQAAAEPSDCIVYDAVSELLSGMRPWHKEAVRGGLLVGRLAAESELTQSEALLVLSRDPRPEFRKFLSARVATALGSAAAAIAFNPPPAASTRQQQQQRHHVPRFDVLYDHGSGAVGDSDAATAARASAHFALLCGRLMRATHGSLVDCALEPATPPLEELLKTPKKKQVASAVRAGGGDLPACVVRHLLRAMALAGSGTMGCGGGSGGGGCETLGAHLRLQLPVVLSVLRASVAATEAFRQCWSAVPLGFFLPWVGQMTSMISGGGGGGGGGGSADDPLLGPLTAFACTYPQRMYAPLRMMSGGCGEGGCLNRSSAAALAEVMAPLLAATESPVVSEFIDALEQMTYPAQRWQVFTSRITQALSRADIDAAVGVWIQEAWPSLFGRYQSAPSAGQPAAAAATAAATAAPIAAAAPLSRRQVAGAVLPAEPYNVTFARRYRVSFEEFFGGSDGAGIRQLAERTRQQLYQQQPSPPQQPLQALGPALTRTFLAAAATAGPGNARAGGQRHWEALQAAGARSLEMLCPWFRSYEQAAADAVTATAVVSAPENRHVRQRQRLLLLPSMAFATASSPPPAEPGMYGPGAGSGLPPLGADDVAPVTVVGFKRMVTVFSSKQRPKLLTLYCSDLSTREFVVKGGEDVRLDERILQLYDVMNGLASHDAACARRSLKVPVYDVIPLSPTVGMLQFVPGTRPLQDCLVDSPEVSEQEAQAIEQYTDFIRSAASARPQQPAATAPLPGPREYWQMYIRADAASTVRHFKQVVSCLGPGRLRASLLSCSGRNPELFLARRGALSAALAAGSVWGYLAGVGDRHPANMLLQPATGRLVHVDFGYSFGSATQVLPIPELVPFRLTPQLLGVLKPHAGREVLTPALAAALAALSGPPARQLLASVMEIFLREPVADWQHEAMVLRGGGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL76</accession>
    <name>A0ABQ5SL76_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015597</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046804">
        <property type="entry name" value="DNA-PKcs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046803">
        <property type="entry name" value="DNAPKcs_CC1-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31535">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31535:SF3">
        <property type="entry name" value="REGULATORY PROTEIN ZESTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20500">
        <property type="entry name" value="DNA-PKcs_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20502">
        <property type="entry name" value="DNAPKcs_CC1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA-PKcs N-terminal" evidence="2">
        <location>
            <begin position="216"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-dependent protein kinase catalytic subunit CC1/2" evidence="3">
        <location>
            <begin position="698"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="949"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="950"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1136" mass="117634" checksum="91BEE22143D4FEB0" modified="2025-10-08" version="1" fragment="single">AEPMARMEGEQGLLGLMRALRARVPATTGIGIGSSGDADAFSGGEDATTGGGWGRLEACGVVLGALAAVVSRTRVLDDAMVGLLTELMDRIVVGYPGIAGLSTLQRRLNGEVVGALAEMAAHWPSRLPALLQRLVAVLMTHTLRDTVPLPAGSVTGGGYGEGSGTTSTSVAPWEVYLDLWRALLLLPSSSSGGRSGALLPLQLQADLEEKYGLTVKRRLRVAVYDAMMLAVLDAIRNLNLCYSLPEPEAAVAAATGRGAAATAPATAETFASSGIGHHDVYGAILADSPIDMMSYFHLKCFLQDLLLECGPGLFVGWALPLAEALAVVVSKRPLVSASYSLAAVAIELADKGGLLTPPVVMAGTAPLRPVAVAAAGAAADIFRGLLLRVAATCSQYKDELLVACLKLLLAAPPHMLLYSLGPTTTATSTARDGALDSAAAGLAVPLALALPLGLSYLPVAEAAVAALERWEVQQPEALQAAMPYFVPLLEPYLGQQSMLEPDDAGDGGVGVGGGGGYSGRSAVGASMSRIRSLKVAADAAVPEVSVQLQALQSRLQLWVGRNPAAIPFLAAVDLTALLAAGPPNGSTAAAVGPAVTRLFQHLPWDEDSTRVALEVAVQPAAEAAAHLRLYLDPLLPHAARLAEESPDPQSRTAAAELIHAAALWVVGANAALPEKLQEGGGASGGGGGGGGRRETRFHGLLRRLFPTVLRLAVGPEPLARDLFRQLAFQLVHWYTRGSRREGAEAMALLEAVLAGLAAGGAAAVATSRGDVATWANGSGGGGGGGGLRDLCASLCAEWLEWSEKQNLTTDGDGDAVSVNATSLLRRLMDRLVQPSPDVRQGAAVALAALARPLSRPDNLHLANGYFLEALWLCIRALRHQLADDSGSSGALATLGSDSGSSSGSLGYGRSLVRAITALVHHCLTRELAQQLCRSVSSYSGGDVYVAGFNDNDDDDGGSEGEEGDGDGGTLASVRQRSWVSTLPTLVLWLWRGTGGLAAATGSAAARRESMRLHARLAGAVLEVRRVRHLSGDDLSGMDRRVNDVMDLEDEVMDAAARVPPTPPMGFSGRWLEWRCVRRGYNKLDVLAPFVLTVTAPPVDGSVGGDGGAQMDTTELVQGYPVDSNNIVGGRGPAGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLG0</accession>
    <name>A0ABQ5SLG0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015777</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="19487" checksum="13DCF4EFFF34F7E2" modified="2025-10-08" version="1">MAHTTRVDNSLNPNFRHIYSFHTREQWPQPSSSYTVFHGERPELPPYIPKYTITPGTAALASRHGSSPYSFRATAERVGPGPDGRATYRYSTSSTGGSLNGTGKLFSNTLVSSGLPPVAYKSYITEYVDEYREPVAHLDTLKTLTQKFNTTGGTNVTKRSTRSEGVPKYESRVVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLL8</accession>
    <name>A0ABQ5SLL8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M41 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015693</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000096">
        <property type="protein sequence ID" value="GLI70810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M41" evidence="7">
        <location>
            <begin position="23"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="113" mass="12367" checksum="FDDF12CB4CDA80CB" modified="2025-10-08" version="1" fragment="single">VDLPDFEAAIDRVIGGLEKKNKVISTEERRTVAYHESGHAVVSWFLEYAEPLLKVSIVPRGTATLGFAQYLPNENLLLTKEQLLDRVCATLGGRAAEQVMLGKISTGAVNDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLP3</accession>
    <name>A0ABQ5SLP3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015732</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S26 family. IMP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052064">
        <property type="entry name" value="Mito_IMP1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383:SF16">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383">
        <property type="entry name" value="PROTEASE FAMILY S26 MITOCHONDRIAL INNER MEMBRANE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S26" evidence="7">
        <location>
            <begin position="32"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S26" evidence="7">
        <location>
            <begin position="120"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="176" mass="19677" checksum="E994068C0C9E5896" modified="2025-10-08" version="1">MAAGGSWSRYQQRIKSWMSAATENTSLFLHTLSCFYVFSRYGLFVSKVTGPSMLPTFGGQGDFVIAEAVTPMWGQLKPGDVVICTRPVDPAESIIKRVVAVEGEEVVLYPDRESSEVRRLTVPPGHVWIQGDNLTQSLDSRQYGPVPKALVRGRVVFQVFPKPGWVDNSMEVVRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM31</accession>
    <name>A0ABQ5SM31_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="149" mass="15905" checksum="E2F996564597B61C" modified="2025-10-08" version="1" fragment="single">VSDLISQLEKLRSGAAMMEARKGGLSGASTDLQGSSITAKFAIIPAASCPAMGATSPPSAVEVADAGAASIFINKAAQQPQELYQPLELTQPPKPHPKTPERRLPPEARNWGLQDCRSWAGEQHWSSWAEVQAFTKTLEPTLAEAIREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM57</accession>
    <name>A0ABQ5SM57_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 1 regulatory subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">VaNZ11_015894</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Component of the PNUTS-PP1 complex (also named PTW/PP1 complex), composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA (or PPP1CB or PPP1CC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46557">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46557:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="18">
        <location>
            <begin position="441"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="17">
        <location>
            <begin position="1399"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="15">
        <location>
            <begin position="1399"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="513"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="563"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="598"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="663"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1032"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1141"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1433"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="513"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="599"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="721"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1174"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1187"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1211"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1308"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="1359"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="1442"/>
            <end position="1453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GLI70874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1467" mass="146366" checksum="4864C853408C0D82" modified="2025-10-08" version="1">MQVEPLLVSKNATCLTTPILLEAGAAGSAGLAMDLIGSTAPISQVLQPGSVYHNPRAHLEGHVITYTQGSADIDSDVVLRQDPCAAIPAGPTGRVADWPTGWSQHQDCAVGGATLVAATLQSNSETTNQVMARETQLQVASSIPPSDGSCGSGISILPTVNGLTVQMPMEKGLTDATNGSESSCSRKRSAAEMQSGAGVGLETCLAGHLPGLVACAAGQELLPSGNCHDCQGDERPCPEIFGAPVGNIGSNKTTGNDHISSILPSSGGGIAATDFLVAAPHEEARAWMAADGAGLSMATATDATPAEPVCAYLGTSVTASMTAATAINSSSLTQNTTDKGGAMGPGSCATSGNGADADLSGSSGIGAGSGGGVAVMDTLAKELGPIMARCGGVSDTRHAMRLAALVEGEERLGGRLTLLTVVQQSAQEVLRLFVQGTGLRSLERWVIQFRDEGRHPALVKVIGCLKMLPIDLTALKGSSIGQTVGKLRKHTNQGVRSAAAELVEQWKGVVDRSVGKGEARHSVKSKADSSSQDGPNKRPRKLIEQPGAAAAGAAAAAAAAGGAGPASKVHSGTGGDGAPMPTTTATSKLAVMDDDLFVSESTGGPVSGGSSLHPTGQHRPLGLTSFITGPKKVHSVKLENVVPSRAGSLGGAYGGSSAVCAAGSGAARTPGAVDAVSEDGSGALPSPTSPRGDTTGALPEAVSGDAAALNPDSPKHGGDGTTASGNSGNSTPSLLPLSGGDGTSSTASLAASGGGSAAPKTMTLPRLGTLAGPSNPTVRLGSLRDGSLPTSGIARLGAAAGIGSGSVSGRTGTAASLPAAVAPGGPQSAHARAMAARARAPSPEPAPRKEKKKAVSWAGDEALASYRLFKRDDPPQAASSDAVLTAEDEAAAAAGDASIVAGSGGGVGGLLPPPGFQSAARREHASERMALIAQHQQEEQERQQELERWAAMRPTVPWVEPPELYLGAVSPQQLPALGEDSTEREVQGRLRQHTPRIMYYSPSHVPASPSEPPPEPEPDFYRVRIIPLNVPSGPQLPMGGPGPGGQPALLPPPPGMIQPGMYGGVPPAQQAHPGLPLPLPYVSQLQPGQMPPQLLPGAPSAPHQPPPSQGPAGQHGKQQGPGASLTLPPDLVASLPALINTLAQQPPGPGPQTAPQGGLGPGQGSVAPGPPGQSGPQPQGVLPPSHQHPHTLPHPHARGPHGSSVPPPPQQQSQPGLPSSAGAPLAGPPGAPQVRSLEFGMQSQPPHTGMGPAAVPSPGRPGGRFESGPGMTPSMGLGQGQGPAGPPLPPPRKDGQPTLMRPRRADEPPGPGPGGIPLGPPPHPPPHPHIMGPGRGGRHAPPQHMQSGGGQVPHVMGPPGSGAGQGSGPGGWGPPGGPGGPSVAPPSNQGMPPPGFHGPPSSKVCVYFNTPGGCRNGDNCRFLHVFPQDGGPLYDNGPYPGPGRGGMGLGRPGGGPPPGLPGGLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMB9</accession>
    <name>A0ABQ5SMB9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015468</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="482"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="485"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="559" mass="59822" checksum="51755B9C0EC1BBE4" modified="2025-10-08" version="1">MAYQVVVSLEGILSAGAVIGKKGRNARWIKERSGGARLKVSEDSVLITAQSASIAAAAARLLRAQIAANRRLASPAHPMRTDVVLGDWTFGCLNPLFRFCRAGDDAAKATGRQEQLFLMSPCSESDSSSDSDLEERGLLRRFMGFSLQATASASISSSSRRNGPVGAQEVRARRGITCFHDSGLDTRVKLMDALEDAARKARANAPSFDVLKVRFNLGKQYFCNLDGLEQRHGLRLKNLKDLQSDASRPQMVFSNFVPSSAVPRITSWLTGFLGFSLVNIKTTATLHVIDDALNVKYAVALTLNETGNRSRLVILRKVKSASTKCHTVVLLSGPEGLDLRIKLLGQRRELQDPKATAVAAQIVQICNSMGYSAATGHGRGGAAAKGLLPPHTTLDTSRKKTKRVYMGTYYPHNNNTGIASHARTCSHACKRRGKLPNLPSLMKLSVVTLEDTATGETHTEITGTIPELNFWLEQLMLRDPGGGNCAADSNSSSGNGSGGDGDSVSGDDVWCVGPVEGLGKFLAALNSGILQGAAREQQFAFTGSNATMGGVSPWYPGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN34</accession>
    <name>A0ABQ5SN34_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24110">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 78 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24110:SF3">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 78 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="456"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="618"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="701"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="912"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1144"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="461"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="715"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="733"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="969"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1025"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1042"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1067"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1144"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1155"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1169" mass="121785" checksum="6EDB409F528435A9" modified="2025-10-08" version="1" fragment="single">MLGSSERVGPRAPPKSDVIVLDKQLQGGDVEVNATMLSRDDLVTWSKKVTENAHRVKSLRILCVPRGTMWSEYHYGQLQEHVGGRLIYSSPEHKLGVKVASANAQHHLARSVFCKALTAVLRHGPVEELVLGLRLSKSGCAALSDALAANTSLKRLSFAGSHMGDERVMWLKPGLMANNSLEELDLTACSLTDEAARAVAAVIKTHVDRRLALAFDEQLRQYPNTSLPRHRAHLEYHAQLRAIARNVDQVCGGLLHLELAENKLTDEAVWALCAALSYDRRLIFLSLRANKLTAAVEGEVISLMKEHQALLRVDLRQNADPKLGILKLKRRVAPRVFSAAIGFQPNPAAPSLLSPARTDTSMPIWALREEMPDSMGPTPLATPRPQAPNSPGHVSAVSWGDLHLLARGAPSAAAGAGHIGNGGRSHNRSIGGGGDVGTNKAPWLLRAGSFGRRLREATENNGGGGGGGGGGGGGITSASMKMVPASPQVRQGQPELATAPRPPWQPVQQTQQLWVDREATLSGNQAPELPGTRTTLDPLHQRAVASAADSDFVLQGSGPDGASAAAAAAASMVTSEPVPVSADNASFGFQILNGSGAQPRAAAAAVVAPSSFGFGIGPELMGQGSPPPRQSHAAAAQPAGVSTTVPLGRSPLSRVTRPAAAGTDADAAGGGGGATNVVPWVWGHPVNPDGSDMVVLSAVMSPSRQQSGGGAVSEPYQQPQLRSNPSAPPPRQQLHSTTPGDITGGASTSAGVSHGGATGRIMPFGSASQQPHLQPGTGWISIPGTGCGSAWLAMPVPVSCPTVSEPLQAAPPVWRTSESDLRHVQPTRGGPASVVADMADIPGGGGYGNNVQYTYRQGTSPPAGGYGGGAAEAAPMVTTTVADNQRQAAFMSMLHREALATGQGNYDHLFTKQRQQRRATDSRASPGRQGGRGSSSKKLLRRSSPRRSGAISGNVPVRAGLKRRKHPGTATTTTPATTRTRTLSPAQRFLSRPRSSSTPHLTPTRHYVRNRGAGGADDSHGGSGAAPFRSRSTGGRTPARRGAGGGPAGGGASTRRKALGWSAITSRKGRLVRAKRTRGPGSARKGGKGQQDQLGRRSAHRHSPNRSEAFRGNRPASADPETLFALERSLLQMAAEVAALETAAAASRDAVAAATEHDAAERAEVVHAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNL7</accession>
    <name>A0ABQ5SNL7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF10">
        <property type="entry name" value="OS12G0136300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="820"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1251"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1353"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1525"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1576"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1699"/>
            <end position="1740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1760"/>
            <end position="1782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1796"/>
            <end position="1838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2081"/>
            <end position="2133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2200"/>
            <end position="2228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="552"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="576"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1252"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1369"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1415"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1538"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1576"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1601"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1727"/>
            <end position="1738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1798"/>
            <end position="1824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1828"/>
            <end position="1837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2081"/>
            <end position="2107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2299" mass="241287" checksum="6C8F6D07BFDF17EA" modified="2025-10-08" version="1">MDLTALHERRRRKAIVAVSILQAESGGNASGIRQGDPISFMPWLMAGATTNFGATAGALPKVPSGERQIVAGSLKASVLSARDIWSWRQQRRCLAARGQSTALASSHRNSNPKPHKAADPSHTPTDLTRVARSGEGAPPSPSATQRSTFNDSSSNTVQRPHHTSAAELLAICSKLHAEVSDTPPLLAPPAAHCQLRDIAQAIRDSSLSVYTQLQQENAARFLRMRNERAVLGLTRSYRSNNSICFGDLVQEYHTNELSVAHWEECKAVYKDLTVRDRTKLTLRDTVCTYLVPEHEVRRHTSSLKTLPEAWQQLVRFGKSKAVAAAVAGVKALRLTVKEETPKTFMTLLNAGRDPHRLPVSHLEPLACAGVYAVTQILMETCPKDGTVNPHELYDILSYIQPPTPQQCVHGKGLSRKPVLPKFTAVLHHSLEHLFHVASRYKFSRGRAYMPAQIVRHDIISSAHLNAMAALGPYGNPIPNCAFVREIHQLPLPSPPVSPPRASTSSTSAPRVLAPCTSARPAAAADDPLNLERHAVRGAKLLASFSAARKPLTGGTSSGGSSISTKSSSSSGGGSGSESNGINNGILRRVSRDLQSRDSRGVSSNNSSSSSSTNIDIDYDSDVSTSTTVRLDKPRVYGVLTAASPSPSPSLSLSSSPSPLPCHLGIEDCHSWGDIEFGPVVGGCSGVADGGGGSGGVWQERLITHKMKKLAAAVAEETRREVWWLSVLDVVDPDLSQALVSDLVLREPVVDAVRHDQRQQQQPAAATPPALMTKQSGLAYRLMMLAKRAANSVLIAPYDDVLGSDEGTALLPCQLLHGLEEEDQSSGTGNEHVTSGLRPAATLTPPPPTAESEMVLLEGEVRSLGLRERNRIKQLSTVFHAALASDVRLAKLVRLVLTWHRRAVISGGSGAGGGRGRGDDGTSFLTECKDVGDVDSDVSDVTDDSERQPAAAATAAAAAAASTLPIKKILDALAWLRPGLLESVHPESWGQLAEEARGHEEHLNPRYGMAEKALRLGLLGLILLAHRPDLPLRQLRLLPCVAAGATYKAWATRLDHPPSVKQGSLTLPDPVTHYQALAAFAAFGFLPGRLHAGRERAAAATAARNGTAYTAHVSTSSPSQAEMAAEAAEVEGVIDKASAAKVFGRGFAQAPAVWTAIGLYVSVQVLADEEGLEQPWLLASAPTAAADDAIAGGGAGTSDVKANGVEAHGLVAAVKASHRPQHGHGRGVDSLLQDLKRDLVVVAARDSLEGTWSASASGHDSGSGSGAMRQPVVSPPLPVGRFDVDSCSDELLDISSTIRRPRGAACGGSAKTCCSHVAAASSSTECADPWSICASAGGGTGPNWLRGRSLAEAFRPGSDSDDECWFPTPDVKEGEKEQEEVKDRGSEKEKDGEKEREKEKEEECEKEKEKDRVAKAEALSSSCSSRPLSASAAAAPGVIKPLPLSQSGGWGSFWKHGGGGGGGSSNSGCVGRHHGSWAMDDTDKVDKGFDDFLTAMITNEMQPEAQALLSEEELFKQILGDISPHLRGGIDPGKDSETSHSAARTAITRATSTSTAAASPALGSTVSPIVTSTATDTASGTLQDSSGVMPPPATVAASSIVGREEQEERAKEKRLEETLHELLAVTLILKAFDHNLKLDGVLQKQLQEQLESQEQLWPLQKEELGLQGQQQHHHYRDYQNQDRSQNLDDEVGRRQLQQPHQPLDAILPPPQPSASRWPPSLPPPPPLSLSSASSMSLCPNGGSAAHCWTPPYAVLRKSSGVGAAGATSGNRRKQLGGPNSNPRQSLAQMMRVWRCAAGRSGGEGGGGGGGRRGGGGGGSRSGSGGEMAPSTSSSNTEYITAVGGPGMALALTALCCGDQVRSSGRGSGTKFGSGSIFQSSGGSNFSEQATSPLRSSVLAATSVARAAVEASRSGGFLHSCFQPIASAKSAQMALGGQLGQRAAEQDVFPPPEGVPGVALLSVRRAAVSEELLDTLAVAAVAWPDGRPAGNGGSCKGRVAGMRPGSELLLVSERHSFKTAAAMARKSLVSGRSGSLRVHAMGVDGLETAIMLLAQIRVEMMKQKRDLVVVVRKLDNQGIAGAAVASDDVDESPAASAAAEAPPQPATGASDGNASENLSGRGKTHAGGSGGSGDSTAAACVGACDHGDGGKTAVDPVVTLLREYDGCIAALKNFLPALNLGLQGRRTQPDIDLEQQLDELASEGDAAAAARQRSNASETHDNVLASAGPSGYSSEFQSRFGSGLGSRFPDPKDGLLARKLCDSIRTLESLMVGHSAVRKGLVADLLVCECELNKPDCLVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP15</accession>
    <name>A0ABQ5SP15_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="118" mass="12464" checksum="A19AAF29A80BA705" modified="2025-10-08" version="1" fragment="single">MSSLGPLGPPPTAPIVNPIMWAPELPVNRPQMHTETSVSVATGICAHLMGAGPPRPEEGAPLERHVVTPIDIQDRAALAHEMTFHRTAEASGARLDPTHPKAPFGATLKNAEGELVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP37</accession>
    <name>A0ABQ5SP37_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_016441</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323:SF1">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="655"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="688"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="731"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="775"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1487"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="1424"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="406"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="582"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1013"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1070"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1360"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1019"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1081"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1137"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1165"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1195"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1383"/>
            <end position="1393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI71300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1696" mass="178918" checksum="1512F41D5EE798CA" modified="2025-10-08" version="1">MDSDGCDDITRKPSIGREEGLRTTSPRSAAVDAAITPPSPAPSEAAANQATTGLFSSGSRRPNRGHKHAPSAASSIFDESDESSEEQNLARRSARMYPRIATAQKAAPQAMASVAAATATAASESSDQPTALLPPLPPLPPLPPVPPPPPLPTYRGRTATVGLPTAHSDISSEVTATTTSAAAAGTATPDSTLPLLQQLRVLQQQQELLLAQQRHTGYQELMQSSVSPEQQQLLMLQQQLLLILQQQLGSTSASAGSAKAAAVTGTAVGTASVGQLSAALLPPSLLGSASFTSTAGKPVGGDINVALSQQHQHQQQSPTPRQKTLLEKYLQQWSEVRPISSVPSATAMDDAAVAAGASPSTSTTPAGGSQISTPAAASAGFVGLTAGVPGTYILLDARQSAAATTSAAESVSGRTTAPAHVPASAGTSMQDAPLPFSSHTGPMPNAEAMTGDSVAIAPPALADQQSSRLTISLDALPAILPTPMPELPGGTVRAPVVPSLRLRDLDPGVPSEAPTQASSRLQHQPLPSAIAPPAQAAPMAMAIAPTLPSLQVAQSPQRHTVGEAVGEAELVASTARGGDEVEGLGVRVRRTERTSGLDEKEKEKRKDLPSDELVAENLAGNIDAIAIFEQDEDDDDPEAEADDELPPPWYRKASVLWFLSTVGVGGILLIVGIVLHSRYKSTYDAFRWCYFFAAMPLMYYALSYLSARVFMVLEWWFFRESLFYLLNVQRSAAWLLFFLGLLPWYQTVFRWAWCGSTPLYPRRCLQPAYVRATDIFWKLLLCLMLFCLANLLKAVLAKLLTTHFYRTAHFNKLKSALEKEYTLQVLSRPRRMVMSERVTAQGGQQGGGGNHYGAWYHLTSYLNYHHHHHHGGGGGGGSGGVSVGVGVGGGGGAAAAIGAGEEVAVAASSVEQGNTGGRSAISPLPDAIPEGMEEAEVEQLRPQQPGIETGGSSRTAALDVGLSMPQSQPPTVSQAEMMNVAAAPLTHVVLSGLAPAAPAPAAAPPAIASLSPAELPLGSPSQPHISSTGAADSTQRYGLQPSLQPQEQLLPPQSATAATAFSINNGSSQLAAKPSFPKGHPSTSIYPSTSAAQIPQPPLQPLQPTSQAHQVPASLQPHAGEPGAHGGTASGLAPGVMFGGGGGGGGTGMAGLVPETSVPGPLPPSLQLQQQRQQQRSGPAGQGPTALLHNDSRGSKASKASKVSVVKTPKRAATGALIEAFARPRKGSEALTQAMATAVVMAKQPKSDTPPAGNILTTAAKQGLHYLSDAELEKVRTAIVIRTQSALISKYADRSDEDMAKELGRVKRFAKALFYNVLPPDSGRDYITEEDFVPFFMVAEGGGGGGGSTLFTDHQKLQPAISSHHQGPGAAHQQNKDGANALVGGGGGPGGRNDGNTKSLARMSLTKFSVDPTRAARQRARQRAAEEAAKKAFAVFDADNNGQVSRYEVKEAVVNIYNERRNMARSLADTETIVQSLEFGIGGAIHFVFAAVYLLIWGVDLLTGFSTFSATVLALTFVFGNSVKNMYESMLFLFVTHPYDVGDCILVGTDMFRVKKISLLYTDMVKFTGERMYMPNTNLIAQGIINWTRSKTKSESCRLVCDIGVAWDVREDIQSSLRSYAKEHPGEFDGEPSCNFRELVDPLKVVLVCSWTYNFPPDDFSRLSPSRNGMLFIVQSKAREHRLHHTTFMDNPGSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLA5</accession>
    <name>A0ABQ5RLA5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018799">
        <property type="entry name" value="TRAF3IP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31363">
        <property type="entry name" value="TRAF3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31363:SF0">
        <property type="entry name" value="TRAF3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="752"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="851"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1276"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1462"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1558"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1809"/>
            <end position="1828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2409"/>
            <end position="2436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="752"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="819"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="883"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="910"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1276"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2409"/>
            <end position="2432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2504" mass="268459" checksum="A9D90C09798F6944" modified="2025-10-08" version="1">MDGNFIADVRLDDSDEVLQLPIVKSRVKKLLQGAVRKIALLGAPVIPSSDDSLEQFLQAAGRFFGKDPAKWDQVGDTKVDVTEEAGKRTTKLTGVFTGTELSLLVENPYFDERLPAREDKPELNFSQKRTPIRADDEWQELISEQPWTASKRRQLLTAYMHAKVEAEDPVLSAEGAHGFWELSINKQHHVDFRIDRLAALLNRLSAANLEVATTTAAAVWGLATTSLSRKALADLDIVSLLLSNLKRSLKMPVIPDEATVAAAAAAAAAAAAAGGAAAAVAAAAAAATASTSGSAVDDVPAGGGRNTSNNSVPEAQRNRFQSFLLGALSVLLIDRNCRKAYVQQEPEFGTLFMLAKNLEGYAPDAASARRETAAKLLTTMVQRDADARRSLIASGALRSVISLLNPKGPGESMIQFCAASLLATMVLDDDAMELIRDRGEAPLMFEACIVLLHSTLGKLKREVARFYGQLTPEDMEATPTFDVELGVRLAEAASQAMWGSAHYCVMMDPIQVKMEHISQLGIMGMDCYNTVALPLSRVAHCITASLATLAANPDAAMLIMNSPGDVALNFLMAMLDAIETQNFEQAGHVKASACAGVAFLACHPIGAEGDECMFGPFRGKLLAMGAFGALLRAALSSVMESECDRIIQQAAAIGLMYLSTMAGAVDSAELAMYAALLTDSDNSEMIEFLMAGMWILLRNGNNRKVLGTSFNPSPANQLAKNMINKLNDAITLHEINDEVAAKTAKLRQVASGGTEAAATAETDADGNVHDGQDGGGDNGREAGGDGTDADAAAAAALEDTEELERQQQQQGEEVEGAEDAAAAGDGTEAAQEGDPVAADADVAGAASVFGVEAEATPPPVVEGDTTVTEDGGYVGGASRAATAGGGGGSRATTAGGGGSRTATAPGSPSRSERGAGRGHSSPRDGRFPGSMMINRSESRDRARRKAEVMKGRMQQLEKRFDKQLKDNWGLETLVSVGESWLPAMLDQDEDGEATDVPVLKLFEFLVASICMFMVDDDGVPERRELDVFRLSAPEGARHKTWWTVDVRAPEADGTVDLDTERALRILLQILGMHLSAAWKSMQLGVLTLWNACCRHPNMERHVVERGVALKLLMVINNPSWPPSLREISAGCLEFFQERWSNLCTFGPGEPHLMPQGLPAEVSGLTMGARPTDGVVPYTAAMVGLINTQVPLMEYRGCHGLARMTYTAPYGCPESKAFLREAKAVATGLGAVEALVALMKRLNRRYQDLGPAGMLPGTFRSVSGGIGVVGSGPSPLAPTPAAASGSAAASRPFSPGEENPSMFERDMQNLEAVQDIYFVSMAALLNMSVLRANQVPIAKRGLLVLLGTNTVFYNRVVALRAQLNATRPGAQSAAAGADALAREEQLLYLCSAILQNISQHPHNRTRLYKAELKGSVALDKVIEAATDVDEKTLTAASFLPSISSSLSVSPSAMSVASGARLVSSGKASKAPSRAGGTLTPSPSGMRVGSPGRRALSSPKTSPNGLIINNSVDTALAGSVRPKVVFPPICERGSFDSGQAQRFTGGGGPTTATINRAGSMHSTRSGADEGDIPEISDSGGSNMRHVAVTDSRYRFLSWIDNTLNEIEPSSSVSVAGRNKALMSSSIADDHTVAASRTTHRKHLWDENGDWLPNEPESFKALNKLLARPLSHLWQEMPEHRARQGKQRWEPVVSEYRELQSDKPLTRPAAKLLSTLPPKDQEDLLVAATQLVGLTPEDRASEDGSRIGSAAVNGSGAGGDGLGIAADDFGFAPPVGGDGAATRNSTLSHAPPPSMANLLAGEFSADIPKPIASVTSWTEGPPHRPSTRERENGRVGLTVLQAPAEVLAATAARKAAEAAAAATTHFGGVGMDINTDGDGAVSDGDATGNKAVPLKVCLHPRRPRQVITFEDRIIVDAASTRPTLTLFEHVEGSKVSEGLFPSYILPNGKRAHMYYSGGTLLDEVSVEAVIPPPRPSTVPQALQQTMPLANVLNLIAKPPGSAPPFIPYKPVPRLVPLPSMHTLTVKRPDQLVAEAFGDLREDNLQLVIQAKKIIKTQTTTRVENIEIKPQEEREPWTLPNSIFKNRVKECDARAFYDAHQVEERMFERDWQRACSKEKFTSMMSRENKANKEGKDEKVAIKEVHGVLLKNWPQVFGAYVYYAAGGTGDPYHMSLNAFTTFLDDCSIADPESQYCKRSDCDTIFIVCNFQPDKKSAEAAVNMENAMMRYEFLEAIVRLAIAKYGKAQQTDDLATAVTMLIERNILPNLVPGALHNSNTFRTERLYNEEVDQVFKKHSVLLKALYSRYRLKPVGGGLRPKVLKLDGWLQFASDSQLIDSQFTLQDAALSFLWARMYVIDEIKDYARYTCLSFTDFLEALGRVADMKALPSSSDLDMAGYDNILEWSLDKERMEGGGLDNKGGGGTGAAGGSGDGGDGPTAPVGSSLDIFRPRASAGFNTPKTRPLYIKLDMFLDLVFRRLYWDPSQPEVPFNYDGLLKLIKKIDKDLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMP0</accession>
    <name>A0ABQ5RMP0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">RecF/RecN/SMC N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_000322</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047365">
        <property type="entry name" value="Tudor_AtPTM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21743">
        <property type="entry name" value="PTM_DIR17_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PTM/DIR17-like Tudor" evidence="15">
        <location>
            <begin position="17"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="14">
        <location>
            <begin position="239"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="555"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="859"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="947"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI58594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1316" mass="147202" checksum="257BE998E49C41F0" modified="2025-10-08" version="1" fragment="single">MLDVKPQLRRDAKLYCGRKVRKAFFPDGRRKPQYYFGTVVECPIFDGEVYYKIEYEDGDIEDIAWEELVQIMMDPGVPAASGALADATNLADAQGMLGGKPVGHQPPRKQPNGLATSSLDCPGPSDVCIGMDEPPMQLQGRTRKRDIKERDGGVDGGQDVNAGAQVRTRQCEPIFGEAGDCEAEATERPRKRMAMGRPVLAAAEEDGRDQARAAMMAPEGDDFGGFTPRTVGLPGQIAKIRVENFMCHQHFELEFGAHVTLVSGANGSGKSAIVQALQVCLGVTARNTGRGTAIADLIRTGVDDAKVQVTLWNTGEDAFMPSIFGDRITVERHLRRTGASSYALLDSRGRKAALDRGEKVKEVLDRMLDYFTVDANNALTIITQDKSRSLLSDKSNARDKYEMFMAGTLLDKVQEDLKVANAQVCAMLKNFEEILTRYKAEKQEELQLSSKVEKLRGADKFLQDREMLERAIAWRAVQQMEDRVAAAKAAADIHGPELLSLYECHADAIARAKQDLLDRQTELAIELEKHTKIMENNMSHVQGLLKAEKNSRINMDRAKRSMAAAQTNLEALQNYKKILDDQLAQDSNDKAAEARRLVEEHQKQITEKQAVVQRAVEDVTAALQAREDARAKVEDLIKRDAACRDMIHRTATNVAEFQRQLRGLGAEQDNLLGIFHAVDLNRLIDQNIQSFRQRPIGPLGALLTVTDGKWLLATEVALGFYFRNYLVSCTADAELLRNLMKRAGYQRASAVTTPFDAPMHSIPASRRPANGFKPLLDILIVHDTAARAPVMNYLVDRAHVESIALVENGRQGNSVNHDCSAGPHITTAISMDGDWYRRKGGLRWVDRNGRVRRQHCRLIADLSEYKAALERDLEQTEAQLDAHREDLAHLTVQLRDARAWEAKCAADLKTANGLKNRVNTELHRLMMQTPEMPRFEDDEAGAMLTQLASVQHELASSQIQLQTAEEALALAEQSHGQAVKQAKEEQTRIQEQMAEGKRLREQQTEAISQLAELDARHSAAVSSREQVRANLAKLHQGVEVAVARLRQALAAANQVCSRSMGERAVQLAREFVSEAVQKRLASKQQRDRQLAAMGEEELRRMHMRTVEASMDYAGLEKQLAIVNSNLANAAAEAGSSDLQELEIRLAAQSKTAATWHRAYKRMEEMMQRFQTSYANQMQSFERLRTSLMDITQAKFAKYLRRRDHDGKLEFNHTERTLKLLVRPKGKNNRDVQAVEDLKQLSGGERSFTTVALLLAIGENTESPFRCNDEFDVFMDDVNRRVATETLLEFAMEHSMFQYIFLTPQGIATVEDARQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMT5</accession>
    <name>A0ABQ5RMT5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Histidine kinase/HSP90-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_000511</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="6">
        <location>
            <begin position="123"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="314"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="789"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="789"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI58754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="828" mass="92379" checksum="121D79961DED8E4E" modified="2025-10-08" version="1">MLLRGLPLRLSQVMMQLRGSHLGCLRPVSCLAPAAFQVRANSTPSVARPFPVLRRRNPSSALLGSFSALAPLRGSPLAGWPQMPGLLRAAATEAAPAPTEETFTYQAEVDRLMDIIVNSLYSNREVFLRELVSNASDALDKARFLSVTDPSVMAGREELEILISADKDKGTLTIEDTGIGMSREQLLSNLGTIARSGTRKFMEAMAAKGDSNLIGQFGVGFYSAFLVADRVRVQSKNPEDPKQWLWEAAAGSHQYTIKEDTVSDLVRGTRITLFLKEDAAELADPVRITRLIKQYSQFIAFPIKVYSPKKEPRKVVDTEATKRKQEAADKKAAEKNEPSNPVDPVLKTEYDEVWDWRLENENKPIWTRSPKDVSEAAYNDFFKATFGEFLDPLAHVHFNVEGTIEFSAILYLPGMAPFEQQNMMQRSKSIKLYVKRVFISDEFDEDLMPRYLSFVKGVVDSSDLPLNVSREILQESRIVRVIRKQLVRRSIEMMEELAGKETGEDYKTFWDAFGRNIKYGVVEDTENREKLSKLLRFSSSKSEDNLTSLDEYVSRMKPTQKSIYYMAADSVQAARAAPFVEAMVAKGIEVLYLTEPIDEACVTNLAKYGDKDVQYDLVDVSKEGVTLDEEGDEEKAAAEAAAKELQPVVEFLKKSLGERVEKVTVSNRLLDSPCALVTSKFGWSANMERIMRSQAMGDARAMEYMRGRKIMEINPKHDIISGIKTLLQEKDEDRARDLAELLYETSLITSGFQVDSPKDYASKVFTLMKIALGYDILSEAEAQQAQAVSTPTVTPVTAAAAATSAPTRPTATPVDAEVVSEDDPWKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMU2</accession>
    <name>A0ABQ5RMU2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosine-protein kinase ephrin type A/B receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000638</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00185">
        <property type="entry name" value="TNFRSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46967">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN,N-TERMINAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46967:SF1">
        <property type="entry name" value="KERATIN-ASSOCIATED PROTEIN 16-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045787912" description="Tyrosine-protein kinase ephrin type A/B receptor-like domain-containing protein" evidence="3">
        <location>
            <begin position="29"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1525"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein kinase ephrin type A/B receptor-like" evidence="4">
        <location>
            <begin position="1312"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="668"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="903"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1206"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1448"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="696"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="943"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="984"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1008"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1206"/>
            <end position="1224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1659" mass="171911" checksum="802A0118C3F46271" modified="2025-10-08" version="1">MGTGEWGKCYTWILQLAILFLWSVRVFPLVEQQAPKLSDSIWGEKAVVLNLSTLKISSDGNSTILANFTGPGGCPTGNLFLVFSNATPTGGDISIPGEMNSQCQTASFFTGASADFNSIQPGTYRVRLLLGLAKRVIPFSATAAQDQGSNNSASLGKIRSDTILITVVRAAVADFLGQSSLTSRVHATVTLTVGLDGPAELPTSLGLRVWDPSAVEVSPASLTWQPGEADSRAFILRLTRAAPSGQAAPWAELVRVENVRFSTGTPPYLEIRAPRPKFFVVPNQVLYRSPNDPVGDDSSDSGNPQLSTSGGVGGAVVLSAPGISQMGSVNVEVRRTDPSPFGSTFQYRASVLQVAGCASPSCAAAVSASGLTLRFSADNTSAFANIAVNWTALPLEAALAVRLQLTPIENAEVVEPPANTVSLYVYGSTHGTCPAGTFGPWQDPAAPALQPRGGNEVATQTALSDAVPPTPPSRDVDAFAARESAGQAALSPPLTSAAPSPPRNPCNAKSMVQGLLDYNPTEAMLPVLSACADGEAVAVQPVRMALSEDEPPPLSAPAPLQGLPAAPKPPQPSPVPSGAGEGIDVKTMELLVPYDQDAVSVFIPLGSVNETVVVMHTTCSPAPKLTQQSVPSPSKAGVVAAWAELTLPTHLELCFILLQVFAPVWSGDATQQQQPQPGGGDRNSTGTALSRPPAPSGGKSSPPTGFSGARQGSSQASAGAAWIADISDRTPPMRTVRAPPNALPSTALPSTAPVASGIGPVRGIVRILSMVPLRKFNVTLRPRSNPDIFSAGALSLYLVHRPSGRCLPLCGLASPVLSATGAHLFGDNFDPVSQSQLDKAGFTLIQAIRGNPDAVALLDEARGAGGGSSERVSAFGHRRILHVGELLRRWLATRLRAGWFDAAGTEGPAAGSRSAQVRLGRGMTQDRPDAFSRRRMLQLNGSSPPPQAPASPPLTPAPTPSPTALAPERAGSPAEDTLPVLAPSSRSAPTTQYPTLQLSPAAQATSPLAPPPVPPPVPGPGPFSDPVPSYPGTSSRALTMLDGFDLAGGLGDGRQMHVLFNPEFVEWCHTADAQVVDLALMTAPYELELEASSLGMTLQLGSVGSDDGVGKGTEVVLGPTTRSQLLQQLRGGAVGRGGDALAGDAMPPESADNCTAQPYLPETDKRIDVWLLAPDGVSTARMKLGLYVDFKAAASRGCQNITQVLSRNSTEAMPSRPSSSSGTGDVSAIGAPPNAVGRPPITAEQLPPPAASNSSQPIYMSLTSPLSLWTACRPCGAGSFSSTMNATVCQPCSPGTASNGTYNKECAVCDPGTFAAQIGSTSCRPCYAGSYAPRHGSAVCIVCPEGTTSLMDGARECVIPPQPTKGNPAIVVSFQMVLNMSATGNSSVVQAASATGMSDAPADTIFRMLIAADTAAALRIPPSMVRVTLMEVSPSTFLVNVSATMPLGQNPSSNTGSSQGGAPPGSTGTSPEVDADTLINRLVGNPEGSFPRTKEATGGSLQVQHVQRQVVVLDDQMRGINPHAVVWPTLAVTALLALALGVACRRYCGRRRARLGVSLPPITDALGRFCTWKYPTSPPPSAHFTYGRFTNLELQDQSPGPSAARHRIGRTCSKSNLPSLGMSLGSDSSDMLTIGSTVTADLDGIAMMYTRDVLAAPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNA5</accession>
    <name>A0ABQ5RNA5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PsbP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002683">
        <property type="entry name" value="PsbP_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040946">
        <property type="entry name" value="PSII_PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407:SF4">
        <property type="entry name" value="PSBP-LIKE PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01789">
        <property type="entry name" value="PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PsbP C-terminal" evidence="1">
        <location>
            <begin position="84"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="235" mass="25914" checksum="2F3CE38B38A243B1" modified="2025-10-08" version="1">MKLRQPNALVPCFVFGKITMALKASCSSRQASARAFGYQRPLPSAGRVLRCHATGDMGRREFLQAASAAVLLSASPAFAAKAPKGYSPVEDLQDSYKFLYPFGWQEVAVKGADVVYKDVVEPLESVSVTLTATDKNDITEFGGLETVAETLAKDVLTAPGTEVKLISTSQRELKGHNYYEIEFTATNPRYTRHQLAVVACNNGTFYTLTTGANERRWDKMKEKLQTTVRSFQLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNI5</accession>
    <name>A0ABQ5RNI5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Maf-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213:SF4">
        <property type="entry name" value="7-METHYL-GTP PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="29198" checksum="AC6F650D9E49A75D" modified="2025-10-08" version="1" fragment="single">AYQKNSFREKKGLDPKASILFCQRIASFATSQTGFMMPSLSPGIQTRTRSMDYHQKLTTFFQSKPRIILGSSSIARKQIMDQLAAEHGFTYEVKAADINEKAIRDPSPDTLVTLLARAKRDAIIQKMKDGGEEMQGLLITCDQVVAHEDRILEKPGSVDEVHEYMEGYSRSPASTVGAVLVTDLLSGREMEEVERAFVHMKPIPAEVRDKLIAEGTVFYCAGGLMIEHPLIEPHVERMDGSICSVMGLPKHVVLRLMVEAAGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP60</accession>
    <name>A0ABQ5RP60_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Surface antigen protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000815</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF58">
        <property type="entry name" value="RECEPTOR PROTEIN KINASE-LIKE PROTEIN ZAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="315"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="359"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="38959" checksum="CC7B030143F6B257" modified="2025-10-08" version="1" fragment="single">LMDNRLTGTLPDEWGSEDAWGNLTGLLVAFNQLSGSLPASWGSPGAFPRLGDVYLHRNRLTGPLPSQWGTSESLPNLRWLSAFDNELTGPLPASWGSSNLVLPNLRNLFLSSNNFSGSLPVDWGRPNAFPELQNLDLSDNAGLDGPLPDTWGLSSAFPSLVQLRLHNCSLRGDLPASWGYSGGLDSLAILTLQGNQLSGSIPAAWSQRFGAPPLTIVLKPGNDGLCGTAPDGVILVSATPPNASGGSASVPAFGATDLPGEFPSGAVLLSSRNAEAWESRGRSSTVRFAGDGSGPVAEVVVSDGGRVQSVAKSAGSGGAGGGTSISAGGGGSGGGGTAGTAVSSGNDGGRTAVSRSGTGRSGGGVSSGSGSSGPPINTGNLNEVQLPAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPB6</accession>
    <name>A0ABQ5RPB6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="606" mass="62835" checksum="1347E1213F278EE3" modified="2025-10-08" version="1">MDGSGYGGGYGQPGWPYPAPAMVVSVLGYPAAMQPYGMAYGSLAVAVSRGSAHYDRVPPCTAVGFRYGMGAVPRAAEGPEAYDELWYQGFYRGYDGEHGGEHVEQTEALYGEPSNAEAVYEEPDCEGDDYGEMEYEDDAEDELNGDEPADGVYDKISNSEGVKMLTIPLVTAEVAVAGGAIRKTGIHRLNPAAPTFLNPSAKTFVPSTKAAEVVPDGSKPPTAAAEAAPSVAASAGPGSSTPQSEKLGRTEHRDNEASGQVSAVQMADEEVGTHASSSQGINVHADKGAGNADSDGMVVQGRCADSADGLSVNQATHPVLPAARGSSAAAEEEIQSQQRQCAEASSLHKPLAVVKPGRACLAIPTSPLSPSICSDSVQGVSEAGDGAAKLSDEILSQSAAQKSAVAEMEEQRPGEEHVASLQVAAGLAAGGPAKTTVTQGGGDDVTAQGEAAESRALTMSDMKPVSTPFQRFITGLFSLFQGPRGEYGAESLWWVLDSHQKNCESAPLEGQVTGCKQQANTSAASPSAQQQQQQQGQQHAIIGPYSSEQMILAYIAQALSHELLVCGTRGCSPPTLMPSADCFRPLGLLLEAAEAGKPYDLLPLQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ84</accession>
    <name>A0ABQ5RQ84_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_001679</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Photosynthesis; C4 acid pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF373">
        <property type="entry name" value="GLUTAMATE--GLYOXYLATE AMINOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="134"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI59732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="522" mass="58032" checksum="B1FC44CE6C745708" modified="2025-10-08" version="1">MVRKEATRLVAALFRATNKGISSSWSVGGTRLKSAMPEPQETKDEDLHHKDGKVLHPHLLNENVMKTQYAVRGELYLRAEQLRKEGKEIIFTNVGNPHALGAKPLTFPRQVLALCAAPFLLDNPRVEELFPADAIARAKKILSAFKGGVGAYTDSRGNPMVREEVARFIEKRDGVASNPDHIFLTDGASVAVRLCLNAMIRHERDAVLVPIPQYPLYSASIRLYGGTLVGYFLDERRGWALSVEEMQRALKEAREEGKLVRGLVFINPGNPTGQCLSQDNLKELIQFAYEERIVLMADEVYQENVYQDERPFVSAKKVMHEMGEPYRSHVELLSFHTVSKGTAGECGLRGGYVELTNIHPGAIEEVYKCASINLSPNTMGQIALSCLVNPPKPGDPSYELYTKEKAAELASLRRRAHMVTDGFNSLDGVTCNFTEGAMYSFPQIKLPPKAIEAAKAAGKAGDVFYCLKLLETTGISTVPGSGFGQEEGTFHLRTTILPREEVMSSFVENFEKFHKEFMKQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQC4</accession>
    <name>A0ABQ5RQC4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001689</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF7">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(9) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="113"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="598" mass="63443" checksum="E09C6C667D5B7CD0" modified="2025-10-08" version="1" fragment="single">IEKHENKISQAGIAKIVAPEGWSPRRQGYSDDLDFIIERPIRQHATGSRGLYRGLYIEETPLSLRRGFRPLALAPENQPPPATDPRDLERKFWKNVTLRPPLYGADVLGSLFDEDCPGWNLRRLDTVLSRVLHNAGYSLPGVSEPYLYFGMWRTTFAWHTEDMDLYSVNYLHFGAPKQWYCIPPASRKRFEGLVKGMLPDLFRVCPEFFRHKELIISPQLLDTFAIPYIKAIQEPREFIINFPGAYHAGYNCGYNCAESVNFGTRRWVPLGATAAVCKCNPDSVTIDMRLFRDLVPARLLPPAIFQDTSSEEEGIENEVVEEGLGRPSAGEGHDHAGGRCVIAGAGTSLADEGVAAAALVPPRRRGRPRKRPFLADAPGGILDTVAVATGPHAKWQCILQDAGCFGGASAVAASAPRGHGRPPKAVTAAAGVAADANVAASTAIANQAAHDHAGSAGIAGSAVLHAALPGPRRRGRPPKQSITDGGGPASAGIAVTSLMAAALPAAPPGLPKRPRGRPRKHPPLQQPRTTVAAAVPGLAVVAPLHLTTFAVAPGAAVATIAKQGLMECGAFNGDGVVVGDMSGGGSSVNAATGPIAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR84</accession>
    <name>A0ABQ5RR84_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007246">
        <property type="entry name" value="Gaa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304:SF0">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04114">
        <property type="entry name" value="Gaa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="632"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="771"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="846"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="887"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="674"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="700"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="912" mass="95249" checksum="57617266EFBFE337" modified="2025-10-08" version="1">MVTLTAESKREKPLLRLLSKIVTKAHMITLWLIIVSILGLLALPLLDRNIRFDEKGLLAGLAHATVGQTSNMASIDIHRISRAAGGAAFKRPGAAAALAAELRHAGLTETQLTRPTVFTAAAIPCRCANVHTIIRAARGDGLESLALVTPIAFSHQRADSAHDGRNQTADAAQMALMAAATLTLHMARGHVAGAGATSGGGRWLARDLLWVVPDLSCGGYQCLEAWIRQYYEDVAQMPARSPAGSGNDDGGVAAPAPAAGVWQAAVGLHQGQGHGNPDRESHAGAKAVAAAAASVAPMIRAGVIQQAVVLEALAGASYDTSELLVVGHNGLLPKLDMYYLLKYTFNFPFSSALWRSDRLSGSQRALATWLESVMPDGLLTPHALRSYSRRLLTGLQFSWHQAVGLPSASHAAFKDFMVDAATVRLIQRNGPSFPSGRVDEQQLHHRALILSSSLELTLRSLNNLVERVHHSSFLYVLTDHDRYVSVERYVLPAVALAVAIQLQAAWSMAAIQRLLSTPPGAAPKPPTAAAPSCLSTALVLSQSHIWAVAAARAAARTAVLTALACCLRALACDPRKLGWLLQESLQGGFGWNAATAAGSAAGRASGGLVELAEATVATVQSLLAQPLTTGQGLLAAAAVLLGAAVVALAVTDATGRLAGAASAPVTTKLDVSAKGKTGWRKHNQVSDTQDGVPSSSLPSPLLPLPLQSPLPPPASSGDLLTDRKVSVSKEGREEGNNAGHVIDGVVSEPGALESALAVHMAAVATWHAERVFLLTAAATAMTALVCLNWAAAVVAGVYLAPLALWQGLTVRQAVCVGSSIRRRWAGPLDVLLLLLKAVVWLSWNPLIFLVLIYGGASAAGIAAGGLSSSFSGSMVQLTVNGMSTVNYWFVSVVAMSFWLFGVGRVGKGLLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRD7</accession>
    <name>A0ABQ5RRD7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_001768</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Fe(II)-heme o + 2 A + H2O = Fe(II)-heme a + 2 AH2</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01665">
        <property type="entry name" value="HemeA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023754">
        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="473"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="534"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI59803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="580" mass="61562" checksum="16EAEC437BD722A5" modified="2025-10-08" version="1">MSGAQSSRCALLLRQFSRLAIESASSSVSQSSSSTLSHTPQIPWRIMDAFTSRMVLNPTHGVGVGSMLLSSTGQVGRLSAPLLRRPGFRSFQGIAEKAIASSGGCASTTARPRTQLGMLRAMGVLSKRIGAAAAAGHSTAATAAGAAASTTAASTVTTAARRLHGGAALSEGLSSVSRLAIAGWLGLCSVWVYSMVVLGGITRLTRSGLSMTEWKLAGEQPPWTDEEWTAEFVKYKASPEYQKVHRGMTLDEFKFIYWMEYAHRMWGRFLGLAFALPATYFLARGWVTRALGRRLGLLFFMGATQGLVGWWMVRSGLEEPEHEWAAPRVSPYRLAAHLVSAFAIYATLVWTTLDLLYPQPATLGASESLLRSTAASRQVLLPLAALIAVTATSGAFVAGMDAGRAYNTYPLMNGRVVPEEYWDGRLGLSPLRNLFENTAAVQFNHRLLATTTLLSVGAVWMRYKGGQQLPGRAVTCLHAVAGITAAQFALGIYTLLEYVPAALGSAHQANALNLFTATLALLHACRPPAPSTSGLAAAAAPLVTPAAVLAVGYIGYVVATQNGTLTQSAAYTGPSSRTRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS80</accession>
    <name>A0ABQ5RS80_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044849">
        <property type="entry name" value="CASTOR/POLLUX/SYM8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31563">
        <property type="entry name" value="ION CHANNEL POLLUX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31563:SF10">
        <property type="entry name" value="ION CHANNEL POLLUX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="22210" checksum="F4A67A9566856852" modified="2025-10-08" version="1" fragment="single">MASWKVAPAGSRRQAVRRRLDSISSRLLHRTILDFRLRLVLLAALTLPFILLGGLVHALASGNPWSTSAYVVYGLLFRVPSLGAVRLDEPAASAIVLNAVFLFGTFGFAILLGLIGEEVKGTVKALRAGGAPLDVYGHVVLLNFTSDATAVALLRQVAVDVSKPGSPLYGRPIVVLASRPPPAGPPLAAGGSGMQRASGGGGGDVEGLMKRVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSH0</accession>
    <name>A0ABQ5RSH0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_002269</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="133"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="256"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="299" mass="29560" checksum="B30EF9F448A2F579" modified="2025-10-08" version="1" fragment="single">GHDGRRGAYSECTPLPPSPPAASAPPPPPPLYIGVNVGAMTGGAAAAAAAAGAATRHTPAAGGFLPVLRNSCVISPRDATTAAAAAAEDELAAAIDEGGGGGSSDAATGAAEPALLESGGPATVGGSRRVPSRLLPWSCPTCTFDNKAHSKRCAMCNTEKPRPGVQGGVEGRSSGGGGSSGGKGESGRPGGRRATAAAAAAAAPAEPTPNASEVPSQQFGQVLAAAENSGGGDGGLMRTTPIIRYEALNEVEPKGLTTVEGTAGSSKRRKIQREPLRQLPPQQQLLTDIAKMPPQPHPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSU9</accession>
    <name>A0ABQ5RSU9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="5">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_002429</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="8">
        <location>
            <begin position="65"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="136"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="582"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="698" mass="77079" checksum="FD0D93662F3B9F51" modified="2025-10-08" version="1">MQLPKASQVHHQRRACWGTGSHQHVPLRHYSKKQRVTLSNVAAPEAPVQTASFLNSNFVTNKEQYLALYNRSIEDPAGFWSEIAEQFYWQKKWEPNHMRYNFDVRKGPISVEWFRGATTNIAYNCLDRNVEGGHGGDVCFMWEGNDVGREKSMTYSEALEEVCRLANWLRAAGVKKGEAVAIYMPMLLELPLAMLACARIGAVHSVVFGGFSAEALAGRMEDCNAKVLLTASGVRRGPKHIELKKIADKALDLCAKAGHQVETVLVYENDNAVPAADCPFTAGRDRWWQSEVEQQPTTCPVEWVDAEHPLFLLYTSGSTGKPKGVLHTTGGYMVYGATTMRHVFGLQRGDVYWCTADCGWITGHTYLAYGPLLNGATQVVFEGVPTHPTPARCWEVVAARRVTHFYTAPTAIRSLMGFGDPWVTQCDTSSLRLLGSVGEPINPEAWRWYYEVVGGGRCPIVDTWWQTETGGHMITPLPGAWEEKPGSATLPFFGVRPVVLDEKGNELQGEAEGVLAIAQPWPSTLRTVYGDHERYQSNYFGPYPGYYFTGDGCRRDADGYYWITGRVDDVINVSGHRIGTAEVESALVAHPACAEAAVVGYDHPIKGQGIYAYVTLMAGTEPSPALRKELVAAVRNSIGAFAAPDVIHWAPGLPKTRSGKIMRRVLRKIAAREEDQLGDVSTLADPGVVQTLVELRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTB4</accession>
    <name>A0ABQ5RTB4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="15782" checksum="D71D439DE1A78735" modified="2025-10-08" version="1">MDNTAGPRVLPLPQAQGHDAIPEDPLGEAVALDQEPGATRPAPTAGTRRSNRQRKAREGASSGVHGSGGAGPSSDPAKSRKRGGAPSSAAAQRSDPNAKRTPTDDSFMPKWNCNRSEFERQKVANVCLRCAASHPTKDCPLLPPFVPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTC8</accession>
    <name>A0ABQ5RTC8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="106" mass="10585" checksum="A193A9486A220B41" modified="2025-10-08" version="1" fragment="single">WLPGDQRGRIDEALGRPPTARPSAVLAAGAAGAAAQPSKQIASAADAAADAATATARATATATATATMAVAAPEKLVCSWQASRAVCVVGQREYEVWAASHTGKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTI5</accession>
    <name>A0ABQ5RTI5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002524</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP-type" evidence="2">
        <location>
            <begin position="51"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="715"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="770"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="959"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="778"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="797"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="813"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1053" mass="113858" checksum="00A59C63EB7F3328" modified="2025-10-08" version="1">MGIDVRYCNNIDSMSRNERPRAADSSRSDLNGMSISSDHSGSHYGQGDAVEGRCRVLGCTKEMALERKYYQRHMICEEHLKSLCLLVDGQRMRFCQQCARLHNISQFEGTRRSCRVRLKHRNKRRHIQKRERQEQEAQQHHEADHGDKAGAAAGGAAADTGRQHAAAEGAAGTPTTGGGRGRGHKGAHRSRRRRNPSDDAFASVAAAAEAGVTVIAARGAGDDMSDNDDDDDGGSAKQRRRKRGACFGHGGAGGTASAAARMPQPPPPLPSLDGLHRDPGPLSDASGSATGARQGLSNRLEQNFQQVRDDGQVRHGDGGAKGSGADEDEGAALGNGSEPQEQVSMAGTSSTMNASISGSLSLGILLQQSDGLRQPRLSGLPEGGGVAAMAAARRGQGLHLMAPSSDLGMLEHAQASPLPPPPAQQLQQQRLPPMESVGLMALHRSSGLQLPSLRPDATDLRTRCGSLPHSLDANQPHIGGSGGAAAAGAHGALGGLAGLVSEFSLSRIIASSTDHMLSMIEEELAREDDGLSWARSNLSYAAGVVSQQEQQQQHDQARGAPGSALEAEVQRGRLWQPPPQQQHSLGLWQRQPPRAGNPPPAQLPQQQQRSFQNTSTSLDDKACLWAAVRQRQQQQQQQQQQRSDNLGHQLSLEGSQQQLLRRHWSMVQDAPHDHQQQQIRAVQGLVNPHQQQPQHQQQASTPADNRLRNALGRSWGAEQQQQQQGGHSGGHMVRPQPADRQQLEAAPASGSGSMQWRLGSLLFQRTGQPEVHSVPDLDGGGGRGGGGVGDLLRYGSAEQQQQRAGAQTGVTPSPMQQQQQMQQQQQVFQQQQQSLLGHHSHLQQQQHHQQQQPQQSLPPRQQHALHLTLQHQVQQLHHQYSEQERGTALSQQQAWGSDRVAPPTGHNPLELQRRHSAERTSRLSLCNGWARHQSTLGLDSESALAPASSEQEQLQHLQQQQQQQLVRDGGNVRGGRTADHGLSEGVSQQLMLLMQNGGARQFHEVGSLPSQSPGLVESLRLQTSRGVDVVYSASAATNAFEMPPPRPWGDTWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTU6</accession>
    <name>A0ABQ5RTU6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003299</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044634">
        <property type="entry name" value="Zuotin/DnaJC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="116"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="656"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="314"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="592"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="439"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="536"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="728" mass="79908" checksum="AF25590B20DEB4E8" modified="2025-10-08" version="1">MVIMLSRHLSLEFDEGSRPGTDDGRLVVCSAAGPIPASRKDPAGYYFHLKALKQAGLWEDPTPAEEDGGAANGAADSASAPATPAGADSDKPPPGVEWKGSKARKRKALKKKDGADPYVLLGLGNERWTATDAQIRAAYRRACLEHHPDKALLNVTDEAEKERIVEHFKVVQDAYDILSDPSKRREFDSTDDFDDTLPLQCDPKDFFKVFGSAFRRNARWSSIEPVPDVGDENLTWEEVSKFYDFWYTFKSWREFPHPEEEDVEAAESREHRRWIERNNAKLREKGKKDEGRRLRDFVDAAYKYDPRVLRKKEEDRLEKQRKKAEKEEARRKAQEEEEARKAAEEEARRAAEREAKRAAEEARKQREAAKTQLKQLRKRLRTFAEGIGGSRLVSEDEVDKLTTKLEPEAIAALVEGLGSCAGSAEALALAQKGLLLEALQGVDQREQEAARAREAAKREAELAAKAAAKEEHRRKMAAMREWTEEELRLLDKACTKFPMGTPKRWEAVAGFVRTRTLDEVILMVKDRQGASATRMKAQEDWKGAQKKPAEVRAQADTRAQAFTDVEVNLTGEAAARISQPAAATATVAASSTASSTAAQSGKATISASTPTTAAAATTTTSPSSSTTGPSPSAAPATSDVAVAAKGSKKAVAADSGLWTEAQELALVAALKQCPKELGTERWDAVAALVPGKTKAQCFKRFKELREAFRSKKQTAGEVGAAGGDDADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTX7</accession>
    <name>A0ABQ5RTX7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminopeptidase P N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_002631</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF4">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="6">
        <location>
            <begin position="75"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="560" mass="60049" checksum="9AD25B9199C31B83" modified="2025-10-08" version="1">MLARVVAGIAAGHRDITQSTTLNFLGKNGSSCLRAFDIINRCYSNHIYTSHPPAGQPIATTHPELLEPHELTPGIPASEYRARRNRLGQLLPPGGAAILPAAATTYMSGVIPWPYRQDPDFFYLTGLTQHGLAVLTAPGAGSSQDVRYTLFIDAPNTNRERWDGATVSRESAKEVFGADEVLWMHEMPRKLQDLAKSTTPILYDTDRVGAYHHSTLGIALRPALELPGRVTPLRPLLHSMRLVKSPAEAAMMRTSAAVASAAVRHCMAVTSPGTTEYGLAATFEYGIKSAGAQRLAYPTVVAGGADACTIHYGRNDKVLAGGQMVLMDAGAEYWGYVSDVTRTWPVGGTFSGPQRDVYGIVLEAHQRCLAACRPGLSIRDLHSLCVDILSEGLMELRLLPRASLAEIRSSLYREFFWHSLGHYLGLDTHDTHLVGHDRRLSEGAVITVEPGLYIPDLPQYGAFRGIGVRIEDDVLVTAAGCRVLSDQAPVAIREVEELAGSAAAAHGASSAGFMPRPPHSGLAPHRLFAQEPLGLTAAPAAATVATSDAGPSAAQRHVMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU22</accession>
    <name>A0ABQ5RU22_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047519252" description="Reverse transcriptase Ty1/copia-type domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="11169" checksum="EAB0AEA0986C7F45" modified="2025-10-08" version="1">MGKPSKAHMGLALGVLRCLVGTKELGLCFGGDGDLAVVSYSDSDWAGDPDTRRCTTSYMFLLGGAAVSWNSQLQRMVAVSSVGAEYQATAAAVREALWLRKLAGGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUL9</accession>
    <name>A0ABQ5RUL9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003678</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06627">
        <property type="entry name" value="STKc_Cdc7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF26">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0283821-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="351"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="400"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="877"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1024"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="446"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="883"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1035"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1359" mass="142938" checksum="DB1F7A12EA3C072A" modified="2025-10-08" version="1">MATGKKVTDRFYFYEELGRGAYGQVYRGMDSMSGDIVAIKQISLAGVPSDSLQTVMGEIDLLKTLNHKNIVKYIGSFKSRTHLYIILEYMENGSLASVIKPNKFGTFSESLAAVYIAQVLQGLQYLHEQGVVHRDIKGANILTTKDGLVKLADFGVAAKLGELDERKDDLAASVVGTPYWMAPEVIEMTQVTSSSDIWSVGCLIIELLTGYPPYFDLVPMSAMFRIVQDPQPPLPENISPLLRDFLSRCFLKDAKLRPDAGTLLQHEWLQYNRRTLRMSWRRDRGQTFRTLVQNRTAARQSPDAHENISSVVTRMMAAADEEASGVDAANVNGLASASTGMLGPLGAGAVALPDGGSREPSAIGPNSEGRASGNDTSNLAAAPAGEEPTTALLTEAPQYSDSTAAGTGSTLPYAPADPNSVASPRVNGADVSRAAAQQAGGVPQIPAVTSSSAPSSASVPTSGADAVAAGPLPGPVTDAGLSVLEAGTQPGPLIGLIQRLHGTVASDGSATHGKPMPRDLASWMDGGGQSYDTSRQLSRPTGAWPSADLVIAAGYADAQGAWGPLEADTVGSSLTSQATLLRRQVRDLVQKMRPGARQEPLSAREHQTAREGPATSAAKAMKELEQMISAPPPPPISPNGPWLGVGEEASYLPAEVKAIFLNEGGVLVVMEVLDADNQKAVEAVLDLMMALVRDDVRLLESLCLVGLVPTVARYTQQPYPVSLRLKAATFVHALCFQRDSTLQLFIAAGGLRQLCWMVADSVASEPSPLSHVAIQCLLQVLQCYATLPLNYICRILAQCGLVPRLYKLLKEAIVVRNKMAHHQRPATSATNGAAPSAAGGAVAAPPADGAAAHAAAGAHHMRSISTAPLLSNGPVRGAATAGGVPGTPGGGGAGSTVHRAATAPVLNGAVLGGSGTPAEARGAGGMGIPGEALTYDQVIYLMDTTVYLLVVLSNADGAVKNAMCTRDNLLQHLDCLRRLQPPHLVEMLRCMRNLTSDPAVLPAIKDAGAIACLVPMLAMAPDGSASVNPGPPTPAATATSAGASATSSGPTAGAAAIIMNTAGVLPKSEVQMEALNALYNLCMYNKKVHMEAAASSNIIPHLCSFAAQCWAAVSSTRLQRQASLGGNAAAAGSQTPRLAQTSLQHVDPASMTPGSPAALAAAAAQWPTVRGFVVPLLLGMVACSATTRLKLFQSHGLEIFLQLLSDEDSRSVLGVLQALDSWLAEDHSRVEARLTQRDATTHLVQLYARSCAAQDMQSKLEALRTMLGRSSKLAVALAMGGLVPWLLAMIQHAAPINRVKILDIVRILYEHYPRPKEFIMMYKIQDVLAMLLKTHGRDADAVIQQAQRLLAAFDINVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUZ7</accession>
    <name>A0ABQ5RUZ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Activating signal cointegrator 1 complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_003308</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18022">
        <property type="entry name" value="DEXHc_ASCC3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056379">
        <property type="entry name" value="DExH14_plug"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF13">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24557">
        <property type="entry name" value="DExH14_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="597"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="814"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="1453"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="1664"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2317"/>
            <end position="2405"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="2405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2405" mass="261123" checksum="3EE5E097FFB7B625" modified="2025-10-08" version="1" fragment="single">MGKGQALTVTEDGQVPRLTTLLRVLVAEQAGPAAAPYHRLDVDALTVSRGSVLEEKRSRVGVGVGAGAKGTTAGSSTTVSSSALPRGAAAEGALLANVFGGGWDKAPRAARDDYDKFLKAVSSAVEGEHSSTDLRELAGVVWRAWAGREPPDTSRGRSIQQAIKPHREALVAVLGPVLRDAALPALWESVAALQGWESKLAPQTPTPPGLAAAKQEAAQQHGKVPGAAAVAAGGGRHPLEQRVEFGADLEFHFSDIAMSADQALDLICGSAAGGGGGRGGYVPYGTYSSEPSPAELRQMMAAATGGGDAGGGGTMPYTMYDDDDDDGETLDRKKLRDVGVVWLYNWCARMLGPKGAGDGGSSDEAASTATAIAAVLLAGGSGDEMAAQLYDLLGDAAFDGLKELMENRKSISRALSAAIARYKELQGGPALAGQGGAGGGGGAGGGPAMPSYAPGVTVNSTSQKLLAKLHRKDAKKGARKSDAEVEVEWLMQAGYQELMDIESPPVAVANTITLANGFELRIGTGEGRQGGAARGALPKGTVRRTYKGYEEVLVPAAPKAPPPAEGELVRIVDLDEWAQLAFPGYKTLNRIQSRIFRTAYLSNENILVCAPTGAGKTNIAMITVLREVGANMRHGVIQKADFKIVYVAPMKALAAEVTANFAKRLEPLGLLVRELTGDMQLTKRELAETQMIVTTPEKWDVITRKGGDVSVASLVKLLIIDEVHLLNDDRGPVIEALIARTLRQVEASQSMIRIVGLSATLPNYEDVAAFLRVNAQTGLFHFDASYRPVPLEMQFVGISEKNYLARQNIMNEVCYQKMVESLKAGYQVMVFVHSRKDTGKSARVLADLAAKSGDAGLLDTREHEKYGLFAKDVRKSRNREMAELFDLGFGMHHAGMLRADRSLTERLFAEGLLRVLVCTATLAWGVNLPAHTVIIKGTQVYDAQKGGFADVGMLDVQQIFGRAGRPQFEDSGLGIILTSHDKLAHYLGMLTHQAAIESQFKKFLVDNLNAEIVLGTVTNVREGAAWLGYSYLARRLERNPLAYGITYDQLVLDPGLDLHKRDLVVEAAKTLRESQMAVFDERSGALYVTELGRVASHFYIRAASMVTFNGLLRPHMKEGEVLSMVAQSAEFEQLVLREEELPELDEMSRNVPYKVKGLGGNDNKSGKANVLMQAWISRARVESFSLTADLMYVSQNAPRIMRAIFEICLRRKWSSMADTCLTLAKSLELRLWAHNHPLRQFEGTPGLGPELLSKLEDRQESMDVWSLRDMDEKEIGAMLRHPAAGSIVKSCVNAFPSLELSAQLQPITRTVLRIQLSIRPTFEWRDRQHGAVLRWHIWVEDAQNEHIYHSEIWLLTKKMCRESESHKLAFTIPIFEPLPPQYFVRAVADGWLGAEAWLELSFGGLILPERMPPHTELLDLDPLPLTALRNPSYEALYAGRFTHFNPIQTQAFHTLYHTDENVLLGAPTGSGKTISSELTMMRLWNAHPGHKVIYVAPLKALVRERMSDWGARLCPLLGKKMVELTGDYTPDLRALLAADIIICTPEKWDGISRAWQTRGYVKKVGLLIIDEIHLLGADRGPILEVIVSRMRYIAAQTEQPVRFVGLSTALANAQDLADWLGIGPAGLFNFKPSVRPVPLECHIQGYPGKFYCPRMASMNKPAYAAIQVHSPTKPVLVFVSSRRQTRLTALDLITYAVADERPQQFLRMPESELETCLAGVKDAALRHCLQFGIGLHHAGLADRDRALVERLFVECKIQVLVATSTLAWGVNTPAHLVVIKGTEFYDAPTRRYVDFPITDVLQMMGRAGRPQFDRHGVAVILVHEPKKQFYKRFLYEPFPVESSLQDQVADHFNAEVVAGTISSRQDAVDYLTWTYFFRRLLQNPSYYDLEATDSDSVNAYMSGLVATALSQLEAAGCITLGGNGDGSEGDSEVVPTPLGRIASFYYLQYRTMSQLGSAMGPGMGVQELLLALCCVSEFDELPVRHNEDKINAAMSRGVRFPPDSRTADDPHTKANLLLQAHLSRLPPPIADYLTDTKSVLDNCARLLQAIIDMAAHGGWLDTALGAINLNQSVTQGRWIDDSSLLSLPHLGPEEVEALEEAGFICLPQLVEALAGTDGTGTDGGGSGSGSRRAAAEGLLVRLLGTPRAREVVSVCDRLPVVALTAGRSPRLLRVRRGGGDSGEDIDVVGVRGNSGGGDVEVVEEWDVDLELSRVRTADKRAGRPRVYAPRFPKVKEEGWYVVVGDTSTRELLALRRVGLEGRATVRLRLPTHNGAGVTLRRVTVYLLSDSYLGLDQQYDIVLDEQANAADVLTYSLTDQEPSRGGRHRSRRTDSSFEAAAPSSTSYLRRTSDGSGVGGGGDDAGRIHNEVDLGAGDGPTEGAGAVVLPQRQRQSQRRGGGGGSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVG8</accession>
    <name>A0ABQ5RVG8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_003586</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="149" mass="15350" checksum="3ACD6925F8741AE6" modified="2025-10-08" version="1" fragment="single">VYVRSSSAAADVTPAGVGAGTSAAQQQQSQEEVFIADGSAGTTTSGAGAGARVGSGPGAMLRYYFHIGSVDSFERKLDEAQRELGIPPSQMVPVKYVDEVSLVNVLLEVAPTLLLMGATYWLISRQMRQMTGMGGLGRGGLGGRNGRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVJ0</accession>
    <name>A0ABQ5RVJ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003534</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045591689" description="Peptidase M11 gametolysin domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="3">
        <location>
            <begin position="140"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="542" mass="58787" checksum="27B522CA2596AAD9" modified="2025-10-08" version="1" fragment="single">MKTSLAPAALVSTFFAIFLFDLEACAGRQLITGSGEQIANGTLLSVTTSSLPEGRDSDEFFLRQNDGQMYRLSFCSGVVSAADLAPNVRVAVTYSSIQDGVMHSCQKPRQLDREPPAAAGAGRRYLFGDEITTPIRPTFLIYIVNMCGYGKPATVTPAMLKEVFFGGGQNPSLSEYYNTCSYGQVSLEPSRVVIIANLTIPCNGNLKGLNFTFPSGNRFDTKSCGSENPVKWQYYLDSVALKKRTAIPTNFNHKVILLPAGFTATKPGCFAGAASIGPWFPSSSNANSYGTGLIWWSTELVGDLNAMFHEVGHTLGMAHAAAVANCSDDTGQCDHTCPMGAITSRQGIRCLNAPHLWQLGWGQPFRQLTDTDLPIGTKQVLTIPPQLTTSKSFVMVDLNTMQGNMKLYVAVRINTQPYDLPYARSQNGQPFVVVHSYNGTAKVSYLRTILLADVPLGEGFIHSSSGLVINFVDWDSRKGASATFCQRAAAMEQKCGDGIDDDCDYLPDEQDPDCVGKMTFDTAPLPPHPWPPSLPPPRRPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVW3</accession>
    <name>A0ABQ5RVW3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">F-ATPase protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_003988</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel; it may play a direct role in the translocation of protons across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATPase A chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00310">
        <property type="entry name" value="ATP-synt_Fo_a_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="entry name" value="ATP synthase, F0 complex, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023011">
        <property type="entry name" value="ATP_synth_F0_asu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045083">
        <property type="entry name" value="ATP_synth_F0_asu_bact/mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01131">
        <property type="entry name" value="ATP_synt_6_or_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410:SF0">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00123">
        <property type="entry name" value="ATPASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="F1F0 ATP synthase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00449">
        <property type="entry name" value="ATPASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-0138">CF(0)</keyword>
    <keyword evidence="8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI61574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="341" mass="35779" checksum="CEA84902E929CE1B" modified="2025-10-08" version="1">MSLQTAVPRVFGSILGRAPVALGQSVLSSSSSSFKSQGFNGTLQSINDHVYAQAFSTGSSAQDEKHTSNNVAAGIKLPGMAGSMLLGKTRTGLRSASMIPFAAQQTMNMSTAPQVQSGATVNSLLIGIYRFWRSQAPMEKPHAPVDDRMLPAIIDASDKRAAIGTWASALFCTILASNLLGLVPTNEAPTSGLGFATGLGVSVWATATALGLYKLGFSFPGHFIPGGTPWPMAFIFVPLETISYSFRAVSLGVRLWVNMLAGHTLLHILTGMALALPFSLSFFAMVPATFAVACLLSALVGLEYLVAVLQSGVFSILSTVYVGEFNSVKLAGPLAKVVKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW21</accession>
    <name>A0ABQ5RW21_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_004324</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057345">
        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057991">
        <property type="entry name" value="TPR_TAF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25577">
        <property type="entry name" value="TPR_TAF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 Ig-like" evidence="8">
        <location>
            <begin position="753"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="9">
        <location>
            <begin position="911"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="127"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="353"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="632"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="857"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1176"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1380"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1453"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1565"/>
            <end position="1849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1207"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1453"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1499"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1565"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1620"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1692"/>
            <end position="1708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1730"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1759"/>
            <end position="1773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1797"/>
            <end position="1849"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1849" mass="192987" checksum="43A7AD1402E1D842" modified="2025-10-08" version="1" fragment="single">MESLAEFPNVLHQRIRLRVDAVRGVLHGVTEIYIRPGNQRAISLHARNLQIDEVRVGGSIVKAQLLPRHKQQSADESSEYSVKDFADYMADDYRAALKAERTQGDLAIPLEPQLRAFSPVASKPLIATSSPPRPLAPGGADATTFNSDRAPAPSTGPNTADFAHCSEAFRHKLLHDPLLVSSLKITVIYRKLGGDASVRYLDGLLTTDVQFNRARGLVPCVDLPTSMHSWELQVEAGLCDVAVCSGGLVSQRLLVGCPNEEGEEGAGSEIAERKQNQLRVAGEMEAAKLEKEKEKADAMDVDGLVAIASAEVLTAAGAAAPAAGETGGSQVAGLHSWQDRQGKGPMQIDAAGTGAGVKGSSWDGRGAGEGAKPKAAGDADLADASIAAAQHVPRRPAAKIPNRTPRPYAKVWYYELPLAVAPCHLHVTVGPLAVVPQYQGSTNQLVESFLKQQQQASGGGGIQDSTVITHFGPRCYMELIRSTTRFFYLPFKEYQIASTVNFPLSQLQVIFIPSELAVTPVQVAAGCIVVSTELLHSDTAVEAALEGKMALAEALARQWFGVILQPRSSSDMWLVEGLAGLLADHFFKAWLGQNEVVYRRFKEREAVLRGDDGQPPPLCPQNQYDDADEGVDAAAAGGAGPGVGLGSQSQRRQRKDPLSQMYGSESLDPSPLRRWKAVAVLRMLEKRTGEDNFRSIMQAVCRNAANTIRTWSPSRGPAGRLLSTRRVIAECGSAAGMAKDVAAFAERWVYGRGCPRLTAGCSYVRRSNTLLVALKQEGGPEVVTAGRIASAQRSKSETSNRGVKVAVYDHEGAVQEVVVERMEAHVDAVVHEIEITSKPGQRGRRKRGGGAAAAAAAAAAAGDDGEEEGGGGTQTTAAAAADEHRIPVQYFRVDPGQEWLCDVVVLQSEAMWVAQLRNSKDVVAQSLAVQGLMRLGLQLESSGAADSSSVIGVLLDCVRSTGVYCRVRIEAARALTAFSASMSTQPAAPNLLAFFYDQCRDPITGVLRPNYWADLGTHMVLQAIPACLATIRDVSFNSTPEAMQLVTDILNNNDNSENLYDDSSYLAAVIEAVGLLRPTHASVLTRLLGLLDDYLLRDCLHPSHNYTVGAACLSAMTSLALSVNPRQGRGAGAEAKALELYASVRRQLLRFSRPPCGTVSIGGELTTTDWLPAGSGNTAVLTLPPSPTKDKDPSTLAVASQPPHAQYNHHHRCHDPKHPRRRQQEDEAQPPHHLLRRAAYQCLLRLEAGEAKVMSSRRGAASGGTEGKPASRVVALALRLLHSEPMPALQLAVMMEMLTLLGTMQARVLEEAREDGNLSEPGALTVPVSSAVTASLYKLALRDGEEPRVRHAAFMSLQLLTSQPPSLFRTSPEDALAAEEAIPEPSNITGRPPRSHATGMPPNAAAAAAGCAAPARSVGTAGQLDGMAGTAAAIAPAPKRIKISTLRPGASVGVGAGAAAPSASTGGVVSGAEGSVAPAGPGAMGQGIQPGATEATRKSSAQSPPSMAPSQPLSQGPAADASTAATVVQSPPGIRTEGGPSPTATGVPVEAPLIGATSPEAAAAAAASGAAKATPMQAAAAARPVTSASTDAPRRKAPVFGTSPLRRVAHSPSPPPRPGPASTSTSPRSGANGATVAGVPQRSPPRDPRLGHASQPNLARGRSISPTEMRPGGGVPKPKPRPAPGSALPTASGRSPSTSTSPKRQLSGPSPPRPPRPTVGFSKDSTSLTGAAAARGMKGLAAAATAAKAASPTQSRASPPSPRAKRPPQPQPSRPSSRGLSPLRGAEVDGGPLVATARAPSAARPSPARAQVGTTVPATAAVPSTKPGPTTSPPREGAPPAAAAPTRKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWX7</accession>
    <name>A0ABQ5RWX7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ERD4-related membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_004639</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1242"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1322"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1368"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1413"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1469"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1492"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1529"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1555"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="29"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="1078"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="1316"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="354"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="617"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="675"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="895"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="982"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="688"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="895"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="983"/>
            <end position="1005"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI62080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1635" mass="178806" checksum="445C0BE029F1FCBF" modified="2025-10-08" version="1">MKAMADIRTQDSTWDSIIGVKNQVSDTTILTGLWISSVVGVAVTILFAVLQRASLLYKYRLVASHVRVKPPKLREHGLISLIDWAIKSISVSDVDFILSAGLDALIMVKMCALGVQLFLPLCILGLSVLLPLHWTGGASKQSDAYQSGFMRLTMANIPQGSHIFWVHVVFVYIYLAWAMFLLRWHYHQYLTIRQHYLRKGNDVNLWRALYEDQKQPLDGREKIRNLLLNVPLIKAFMPSFVPASAGSSTPAQGSDDEGDVYPRGGLAPADEAAWKSSPRDSLAPLGGVYSSLRPKDGLQFRAGCLSVTASREICTDAASNLTRRSLPHSGTQSSYVVTPRTAALDGPERILRRATQSFGSPRAAAAAGSDQTMRHVLQKQQLQLRYRSRRNPAPSFRELPAQSSELAEEPSAERIPLPESNSQGIEKAGQRESGSRRGTVAEATEGSQHDAEHDPEVAVAKTEITTAAAVAATSMAETGMTAVPHVDIVPERTQQLMCPPPPVLMPCNSSKLALSHEICGSTRLSQHDGNGSVALQVPCLDVGVTSLGSSVPAAGGTCLSGDCQDSASSESAMELDPDAHLLEWWLERRKMVVPLNGRTPLQTQWGQLEATLSQRASIEPDSGGEVQKSGLSDVDLTRPSVGHRKTVNAEDWDGRRVSVLAQHYAVLVTDVVERSPGEDMHSGKSGVQGDTPNATNGTVPYRSQASCLPRWLRSWCNLQYGKQAARKLNPKLSSTSFDDNFGSGVNHIAHNSRISCGLANQCSRVRGSLASTTFSSPKHSHSRSVGYPLSPPTTLLELGSVGARGDLGGDGAMPGTRSLSGLRAPASLYLAPSASNGRRAGGEGLLRLFEMMAQGAQEKLAAMGGMRTGGPGQMQESNHIGHVATTNDAAFAQSVQTATTPTPSASSGTAQLSQSLRSRSLGHGPAASGGDQVQRSKDRAVTMPGGAVALLPSSTAWEQLEYSRGNGIGACAVAEALELSEGRQGGGAAGGNGEGNQSGSGGGDDGATSDAAVSKADSGMKEDLVAGASVRRQQGVADAANPAELEGGKAASIGSPRAAVLARLEVVRQAVWSGRVAQLPWRYRYSVVSATFLRLFPEEFDRAIQVINFKTVDLLLMQVDRHMAQYEYAIKYEEQTGKKLFGRVGFCGLVGERRRLREYHLEKINTLLDKVRKARVEAANTAQTSSWIVFFSTQRAAAMASQCIIHAEDNRRFRVHPAPGPDEVNWSALWSNFRGRDLRRNLMRPLVVLMVVFPIGIFTGGLAQLDYLLCPSHNCAILQEGSDIWRSSNCTEATKKRENQQLSWDWYCGQKDPFSKLLKRLVVAWLPSLLLILWQGMVLPLFFTFVVQVSRQARSLSEADRLVAKNMFYFGIFNVFLGGVVGSTIIQGINSAIEKGPSEIFNLVGTYVPTSSNFFINYTMFRVFVSVPLRMLWPHIGIRMYLIRRYLRFSCIITQRERAFLMAPVSPRYGFEVGMVTIIFLIGFAFSVVSPLLLVMTVFFFVISWLFWRWSLLYVYVRKYEGGGTMWPFIFNRVMVCMAIFPVFTACVFITKHAYVQAIVLLVTFPIILIRFHKYCYYRFETGLKAMPLEATVAAPYARVGPWVYTPPPLLTNLAGWHPDWSKCWMGWNMPVMYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX82</accession>
    <name>A0ABQ5RX82_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="558"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1346"/>
            <end position="1373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1482" mass="165529" checksum="AFB2ED4EDA402334" modified="2025-10-08" version="1">MSRGNKRSQRQGAISGHPLRIATHNMRGLLASHGSRLQAAVRQWAEQRLDVVCLQETHHTTLQHQCLFERDLRYATKQIHTQNWKIAAYSWSNSTHTAGVLILIRQGLASALAPKLATPQHTCTRGRFTDCILHWGGHRLHICNIYLPPGTTSMAAETRRQLFENALAATTTQAMEDNRDTTLIWAGDFNFVENPEWDSSAGRDGRQADRPITEMRQRAGPQMVDTHRYLHPRRRDFTHLYHAPTPGGSRLDRIYINEAKLPYLCHTAISFHTASDHRLATLHLLPCKNEVKGPGIKRIRMAFTDHEDLATSMAEWIRNQCQEAPSVEENPGNLIAWWPGFKRRLTFLAHQLNKVAQQRRQQPGAHTQEAYLGVQQAQQALDTMPHSPHLLQQLIDAQARLARVHIEDNARVAKKTRVHWLHAGERPGPALTTFLQTQIQDQQTAPVALQAHSGRLVTNPAQLPQVVADFWAGVCRAPDFTTPEDRQSVLTQLAAANIRLPEEADEVAGSPTIAVTELGFALKKSQPGKAPGWDGIPTDLYRKFWPEIGHTMAALFTAIGKTGQVPTGFLDGIIKVLYKKGDPKQPGNYRPITLLCSDYRLLAKVLANRLGPALGRIISPEQSAFLPKRLIGASVLFLRHLPHLLRKLNRQSLLAFLDFAKAYDTVDRNFLMGAMEAMGAGPQLCSWTRILLSNTRARAMVNGFKSRLVKMEAGVRQGCPLAPLLYLFVAQALFSWLKSQEIGLRLLPSDEDRVTACQFADDAEVALEGEESVPAFLEAMDTFARASGQRLNLDKMEILPIGAVDDHHQGSTINGLKVVTTATALNLPMDNQLQPPQPDWPEMLALAYTRMQRLAHYPLSAFGRAAAVSNYCLQTVTWHMEHGGPPPDTTLTYIERLAARLVDRKQGPEDQGQRFTGVPSRLLVGHPSTGGFGLLPLRQHIRARQAMWAVRFIRGALMARRERERVMLLDTAMNTVAEEEEDVSNPPLAPGDWCWAAALWGNPLLPNNSDNGGSINGNNHRPGLETRHQLMASCRKLLTVGDLVRVYDMLEHDQIPLPQYMTWLDWTQQFLDPSASPTQLRDKESTRMALSALCNDISPAWIAAARKVVNRLQPQQPDRVEDRMQQQMPAPPDRTTVMAMIVRRLGWRLPGDSGIVPLYNLTVSQATQLQGVSTAEERLELLSAFVQEAQGMPTDGPAPPCPVTNLQRFQATLDRLWTAVRWENKNKETLWRLVVDGIPIPGNTHLSRVPIKPCGCGSYGADTDQPCSPRLHHFWECPIAKAVREQIDRHLPLPTSATIEGGLTRQQLWLVQAPPGCEQAPWDVIALAALSSMEYGRHCLRAATREHRPQWPSTHRRRQQQQTHTAEQGPRQRAITDFFNRSGGFPQEEEEDANTHPIISTTQPAMDTPTQTATSPLDRAQARTVADFWARLHSFAALGIPHYGWDQVGPQHPILAIVGTQMRCADPVDITNHGAARTEQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXB6</accession>
    <name>A0ABQ5RXB6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone-binding protein RBBP4 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004340</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="417"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="173"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="511"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="541" mass="57207" checksum="8109A52FBC46471D" modified="2025-10-08" version="1">MDSAAELFKSLSDEDCNGRDTALLSYVQVLQRWKVWLDNVPLFYSACVQFTDVWPSNSIQLYPHIRPEPTTPGLSRNAVLYCNRRESDDVPLLILVTCLVDGLGKSRPTQLQQDKDAFRRVCSFTHTIGDIDRLRYCPQRPTLVAVKSAGTALSLYDVSPALSLLDSISSKEHGTAAAAEEEDEEVGHSIKAGDDQSRSNETGGEDEEPTGAHGSGTDGGGPISVLESPAQEDVSLAWGCCGPSGGGDDGGMRLFSGLLSGAIGEYALREDGSLAEVTVRRGGHDADATVVDLAWGGPCDPGPHMPTAEAGTRTSYGALAASGLLGSAGTDGRTLLWDPRQRQPALQAPRCRRDVNTVAFAGAADAGGCGDGTGPLLATAGDEGIIRIYDIRMLGNCGASACGRLSSTAGVTALHRLKAHNCRALQVSFSPHSRSLLASSDDQGRVLLWDLDRTTRLDEGLNTRPQLLFVHAGHPLPVDDFSWSTELFGTLASVSGSINPEVVAAARAYPGAESDLPQPPSAVQVWRPSGNILPPDPHCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXG4</accession>
    <name>A0ABQ5RXG4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Damage-control phosphatase ARMT1-like metal-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004675</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1700.10">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.285.20">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10880">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035073">
        <property type="entry name" value="At2g17340_3_helix_bundle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF35">
        <property type="entry name" value="4'-PHOSPHOPANTETHEINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0533">Nickel</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Damage-control phosphatase ARMT1-like metal-binding" evidence="7">
        <location>
            <begin position="56"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="376" mass="40695" checksum="31414DFDFE3AF805" modified="2025-10-08" version="1">MVALHQLVLDGYNPCTFDYGKQTDDLPTLRDWTEVFRKSIPTFRIHALTDLNVSEELREAAAQQFEVDFNAALDALLEVPVAPAPGHPHSHPVNCYTLCKLREDCLHAVGFRDIFAAVKAAENDRALQLLPSVIRELDELEGGFPAELELALRGVFAGNIFDLGAAASAELHAAGGGVFASTRAQLLPRPWAVDNLDEVLAVVRGTRGTRWRQAALFVDNAGPDVVLGMLPLARVLLKAGTKVLLMANRGPTINDITAGELVPLLRRAAELDELLQVAVASENIRVVCSGSDLPIIDLTRLSKEAVDAAADCDLIVLEGMGRAIETNLHARFTCDSLKLGMIKHPEVAAHFSKRLYDCVCKFDRAAQQASPAGVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYX8</accession>
    <name>A0ABQ5RYX8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="225"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="319"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="322"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="410" mass="42504" checksum="549506EAB3F36575" modified="2025-10-08" version="1" fragment="single">PDCMLPYSAHLSYLDLAHNNLEEMPTSLQVLSELAVLRVSYNKLLRLPTALLPGTHGSLSSHVLSSHDDSSAAAATAATSSAAAPRPRQRHNLLRLLSPPLPAAVPRHPLLVLDLAFNCLAELDHGISRLTSLHVLDLSYNPLTSLPSTFSVLGRLRSLSLRGNLLRSFPPSLTALSRLSYLDVAAQMTLRQEPGRGVGLVGRREEQRLATSCLQDQSLTWAPWEEPHTAPLPSGSSTALALSGDVGEGDDEQAEGAQYTGAAGYSEALQQALGVMASTTTSPAKPPRSGGGAVGSVAPAGGTWSELLGALRYGLQHPAAARRPDRVEGNGADRDGGGGGYGRASARGSGLLSVTAGDEDGGEAVRAHGRSGGDNGESRKGEIGPALRSCGPCSLPAELATLPCLKVVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZE8</accession>
    <name>A0ABQ5RZE8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="26">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">VaNZ11_005010</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26 27">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961:SF5">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 1, AXONEMAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain linker" evidence="17">
        <location>
            <begin position="909"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="18">
        <location>
            <begin position="1440"/>
            <end position="1766"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="22">
        <location>
            <begin position="1931"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein 2 heavy chain 1 cytoplasmic ATPase lid" evidence="25">
        <location>
            <begin position="2273"/>
            <end position="2363"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="20">
        <location>
            <begin position="2435"/>
            <end position="2696"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="19">
        <location>
            <begin position="2710"/>
            <end position="3054"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="21">
        <location>
            <begin position="3084"/>
            <end position="3305"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="16">
        <location>
            <begin position="3550"/>
            <end position="3663"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="23">
        <location>
            <begin position="3695"/>
            <end position="3835"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="24">
        <location>
            <begin position="3841"/>
            <end position="4152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="2753"/>
            <end position="2809"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="2940"/>
            <end position="2988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF22597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="GLI62391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="4157" mass="466329" checksum="2CA731B4695F522B" modified="2025-10-08" version="1">[encoded data, 4157 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZR1</accession>
    <name>A0ABQ5RZR1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006043</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43619">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE YKTD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43619:SF2">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="120"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="271"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="443"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="508" mass="53515" checksum="DF10E941D4AAC657" modified="2025-10-08" version="1">MQSLSQVDVAHEAAGFTGRFTAGLRYVESTCVPEPILLSDPLAGALAGNRGLTLAKQELEQLTRNQGPGKHLRVPARSRLIDDMLGQEIHELQNPLERRSARPASAFNSALAGDAVVVTSARQDGSQSKSGSNPRSGSGAGPESNPRSGSGSGSDLNSPARDGGSNPSSVTHAVCQVVSLGAGMCTRPWRLPFLASCHVFELDLPHITRKKAELLRKAGAQCTARQQKPVAGGGSDAAVAPAAMAPSVAPISGSGAAPGTTTSNRVCDSRLHQEEGSSAAEQQQPWQNGEEEEGDGEEQGEAGEQQQQHQQHKLVFPLLAASYTCIGADLSAGIPGALTASLTDRGFDPATPTIWILEALLYYMPLAAASELLCQLAGLSAPGSRLIATCVDLELLEASLRGVPKGHIFADLWHFEAEALLYSTTAFRDFWQVDWVGQPEAEVGNRGVEDSSSRDSPAPPRRLPASTRRLAKERLGVDTYVALYGGSELLFAAGLRQPPGPHRIAPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZW4</accession>
    <name>A0ABQ5RZW4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF27">
        <property type="entry name" value="MACRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="11"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="240" mass="26159" checksum="095A8453340E985B" modified="2025-10-08" version="1">MSSAPGNQPAVVKKEYKLQAGPKLIIFKGSIPDWPKMQQERPDKIKPIAVVNAANQRLCGGGGVDGMIHSKAGPKLAEECSRKPEVRPGIRCPTGEAVVTEGYTFNTDYIIHTCGPVYAKEREQECEEHLTNAYRNSLLKAQDLGVRCVAFAAISTGVYAYPFRDATKVSLTVLKEAKPPLEEVWIVLFTEGDFKTATSLAEELNMTPYVAPTEGMQGKQEKQDAAASTLRSFGLLGKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S039</accession>
    <name>A0ABQ5S039_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="155" mass="16631" checksum="FAEF10E94C6C90A8" modified="2025-10-08" version="1">MATQLPSPYHTVYGATYLPYMAASIPVSTSDVAGYLGVNYGAQQSIVHTSFTSFPSGAGYGNKESSAHSCTGRSRAARRPADDRLLGDRCTTRGTRTSTAPDRLGKAYKWGKWGKEQYPTSSEYTSSRIRSGISSRKGVGMGTNRPPCSGCSGWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S079</accession>
    <name>A0ABQ5S079_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Branchpoint-bridging protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_006024</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BBP/SF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02395">
        <property type="entry name" value="KH-I_BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032570">
        <property type="entry name" value="SF1-HH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047086">
        <property type="entry name" value="SF1-HH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF45">
        <property type="entry name" value="SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16275">
        <property type="entry name" value="SF1-HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="14">
        <location>
            <begin position="418"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="522"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="623"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="230"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="698"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="706"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="748"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="824"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="873"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="906"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="944"/>
            <end position="953"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI63119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="967" mass="103753" checksum="DC6A86384C5ADDB2" modified="2025-10-08" version="1">MEDQPAEVPEAIPDDKLEVDEPGDGYVEQNTHSLGFETQEHVVATPVEFPPEPERVPEPEVGPVAEPASANGTEGAEPGTHGTGAEETGQEPKRRRRKWGPPAADAVVAPEGGGSGEGTTAASASTPTPPPEGLTGDGDGSEADRKRRRRSRWEENEGAAAPAASTALAVVPAVTGAGAMVAVFPKEVVLSGGIKVHLPTTITGETTMSNVDPRIRTLTDELETLNKRLQNNELDIPPEGDPRRSPSPEPIYDRNGIRQNTREIRYRERLLDNRNRVVEELMKEDPTFKPPADYRPKKYWKKVYIPQDAFPTYNFIGLIIGPRGNTQKRMQKETNTKIAIRGRGSVKEGASRDPKYDYGEDEELHVLITGERQDDVDKAVEMIERLLEPNDDTLNEHKRLQLRELAALNGTLKDEVACYICGETTHRSTECPKSQAVEIYKLPDEIRAKAEEQYARDVARMQGDASGGRGLEDEYKSFLRELGGAPPPEMMGDLAALEARESGARPGLGRPRRPGDDLPEDHKLYIAGLPPVFNDVMLKSMFEPFGNVLYAVVATDGAGNSRGFGFVHMPEAAMAKAAKDGMDGRMIEGKPLTVRLRSERNELRQRGTGGTATGGVEGVDDACKLYLGQLPSTTSEQLLRPLFEPFGPILELRLIRDRETGGLKGFGFLTMTTPAAAAAVMRAMHGYRIDGKMIVVKQAGAPPGSGKEEHSRDFSRDHNRDFHRDIRDYSRQGGKPDGPGRMMGPMTPGGPPMPPGGHYPPGPVPPPGGPPPPPHGGPYPPPGPYPPSGPPPPYPPPHPHGAPYPHPGGHYSHPPYGGPYGTPGAPPPPPYGPGAPPPPPYGAPPPGPAPAPGGAPYHPYGVPMPPELAYGAAPPPYGAPPQQPYGYATAPGGYPPDYSAAGYTAPAPPPPPSYPPPLPPSQPPPPPPESAPGGADAQPPLPPSSANDMQQLSEYERFMAEMQRNYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Y0</accession>
    <name>A0ABQ5S0Y0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006531</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="416" mass="43158" checksum="2DFD207724093EB7" modified="2025-10-08" version="1">MGKSVHVVLLAGGYVPDIASGVAAAVEAAGAPGKLTGALAAAAAMGRTVVPGLLPLDGTPALVRLLKGLQGVRRASLAAVWIVHNEGDTDRIRGGGGLFGPSPASDLGLPTVNFLSNGATEPSNWRGETADFRSALTAILKSGSNDACVAGISCTLAFMPNYNLQRLLEHSYLRGRNVLGFSLLNGVDLTLLGSQGHTELIPGEETALPRIQTLRPLVSPNPGALVAEPYLFLRPETVAAVVSGAGGPGGWLQGLGGGLVEAGVYVAGVDLMFGRYNMRTAEAVDYADRFFAFCSTAATDPAKVGLKPDAVLRPHDTTPGGVKDPLPPVPAAGSFIGRSTAANFRFDVLDGTAKDAESSLHGTDYEAFTKMASTFNSRYFGDTAASKATAAGMKYYLLPPTFYMTAYRRLGADIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1D0</accession>
    <name>A0ABQ5S1D0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_006034</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="586"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="937"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="527"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="670"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="841"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="890"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="527"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="891"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="964"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1362" mass="141560" checksum="C09429DB11D16339" modified="2025-10-08" version="1" fragment="single">SDVTLDPLTWKPVNTGNVVLGLFGETSRRTCLDFGGIPGAFVMPVYNSQSTVVRIQGLMLRGLPYSPVMRSPLDFLSIWIHAFQINRTTPARLDLRSPQVLCVDCVLVLNDEEYSWLGTNMNSAGAIAPLDRPWLVFMFSTPVLGKFAPIMYVDNVPRGISNATAVTDTDLLVRLLTSSLTSYKVIGAAPGSGAESWPMGSWFMNASRDLAPLGQYWVSNSSELAPTMAAANYSKVIARTDVMGRPFLSAMSGNAASGSGGFGRQFVPRSTDVGIYAPPNEQVRQLAFWDMQGVTGELYVVGSSNLTVERIVLYNLAPGGALPYGQGFTYGDNLRQWLAYGTGWQPTTGLPAVGAEDAMANLTLPLWYFAKQFRSVFSKGVLNALDRPIVLSKCLLVVPQSEFAVLLRHLSLAGKLPDTAGAPAGLNSTSHITGSSSSSGRRRLAQVPAPVETDVAAAVSEPGKADMYLRDFASQSEVEWYTNDTLFFNNMTFWRIKGSQVAISYVLPPGAPTTALLYDVTYDSASYPPPSPLPPSPPPPPPSPLPPLPSSPPSPPPGTSFTFLVMPPSSPSTSSMAAAAGSPNIAAIHGAVSASVGVTVVIGIVLAVILFRHRRHRRRRQQQGRQDFLPAARGDGDYRRADWSNGHGNGVHGPAFDAGRATAALALGPDQTRQGEGNGAANDEEDNAQGPQDNGTTIANRAYDIAVPLSVDDSTGAFRLSMEQLHAQAAAAQTPADTMSLLAAAATTTASSFLGQVGTLLSDTSDVGVGGGGRATSLFSALPHIHIVITDDISNGAEGGGGGEGDGIQTVRQQDFEAGATAPADGCSDAAAAVSGEPGGGIGGGTAAPMAGPSKIGSLQGGGQSLLQTMDIESALQAYSSRSQVLEADSQRSTASVAQQGTAAGSGTTSVPQRTDVDLGSAISSLQAELSGRQEQLRITTVLGKGSFGVVYLGTWRGLRVAVKTLVVHDALLGTEGRRRQRAILEAAISTSLQHPNVVTTYAFEVRPLGVVASPPATTCGGGGGGGVDFRAAGGAAGGRSAWECAGDVYKLYIIQEYCDVGTLRDGLRNAVVGSVSAGGAAALCALTLALDVACGMCHIHAKNIVHGDLSSANILLTSLKSSALLNGGVDGAGVGPNNVFGNLANAMALGRTPGSRNLAAQISRLRGLWRPPVVAKVADFGLSVRMGEGQTHSSNKFQGTPLYSAPEVLMRGHLTKSADVFSYGVMLLEVYYGTNIADLKAHRGAEGLNEATLNASIAGGRFGVAIPSSCPVHFRNLMVACIAGEPALRPTFEQVVDSLVDILLMEHVNAQHRAISQMGIPHAAAAAAATPLGADRAIAPTRTAVAAAAAVSVSLMPSTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1X4</accession>
    <name>A0ABQ5S1X4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_006619</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12 13">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF758">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="79"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI63609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="583" mass="64667" checksum="90D05A6633598990" modified="2025-10-08" version="1" fragment="single">VLSKLKTVLHARTDDNISVRHDLCGFIRLIPPYGKTCFHNTFQAFWVPLSLSCITIFCCNSSLPTAMTSFSRRDPVPPEGATHLNCYGYAEPIFLCLSHMPEVAGICLKSDHCTACEGISCNSRRSVQIGGPPFKCVMCTMKGQMIRHLASCGRKLEDNLEGIPTSFTTEMVHLAATGNDHRVFMEEVLRGAAEDEKAVAPKGSVSSMDKLFNGVWHCRTICRGCGYVWDEPMPFKLLSLDIEKATTLQEALSENFGLCDLSREDRCERCQGCLVEECAITMAPDALIIKLERLEARPKDLRHVDFDFDFNLDLAPYMAQYVSIPGGTRYQLTGIIEHRQEYLSTSYYTACVRGQKGEWRAMNNILLHEVSVEEVRRRQPYILFYSRGDAKLPSQADVLEAPMVPPGNKEATTPFAAIDAQLPKTPPRQSVESPMPKTLPPKVPQRVQRQLQLTPSKTGCAPSPPQPTVVEMAPTIATKQPTLATPPLPHQPSSMEVPPLQQSIDAALLQPQEVKEAPILTPPRLQVPRRAELQSGLFPPKTPSTQQATVAKGSQQTMAMMMQQPAVVTLPHGHATHQKRSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1Z1</accession>
    <name>A0ABQ5S1Z1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="167" mass="18425" checksum="C8C4079A0CB007AA" modified="2025-10-08" version="1">MGKHIIPAIANRLRVSRGFDSAWFTGPEHYAKMLQRDWLLTKNAWSFVQRASRSAPRTNKEDPATLAAQAADPSRPRYPKKPQPRQQDSRDFPNFRPARVFSQHMPYKSIVSVFAYQAPKDGSPQAKYGLFQAERPARTSPSTPSSASPSGAPAFRGAAISGVGPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S242</accession>
    <name>A0ABQ5S242_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006683</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="515" mass="55829" checksum="9859A08B5E916C9F" modified="2025-10-08" version="1" fragment="single">MLTCTAAHYWRTALLVIALWASSGGPLGVFASHFRSGIVTYALNRENSTLLDVTVTTTWRYPSKNGYESFVYLQNSGGNDTLVFNTSEDVVVGYGQDPFFKGYIILRTTRSIPVPTDLPATIYISECCRVLDLVGYGDYYGYDDYKHFAFSTQYVAEAPQSIIVDVPPVTITVNAAPGDYAYFFVPAISPTGAPIICSINRALAFTAPDELTATEVNGGCSIGWRNSGYNDGTTVPVGLRVMDVTTGQFNDLTFLVMVVGWNLLPAVDKITSGGMTLPQNGGSVTMYVGEQSQVLITATDPTGADLNATGSSLPSGATLNTTRGSIPSSAIVTFTWTPTSADNDRGLVVIVIASSLNFYTTVSFSYIVTSRPPPPPSPPLPPPPSNSLLVDSFNFTNSCFRGRKASQSPLRMLSPRGPIVDNDTSLTNYCFSITKNTSMVGVASMCRFMNSVRNLHLIINANCLPDFTKFKVFATIGNEYDDTYPITSTSIQWKGTTYGMLTIKRIDDFYFTLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S299</accession>
    <name>A0ABQ5S299_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006563</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="104" mass="10887" checksum="7A776609E012EB05" modified="2025-10-08" version="1" fragment="single">MASSSDEPRGPLDTFAEDLGRAVLDAAASGNLSAPALLAAATETGFRPDQLATLIAQTTEIRTQLGNYLLSLLNLLKSTVVNTNMDNTAGPRVLPLPQARGHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2U4</accession>
    <name>A0ABQ5S2U4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007386</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029159">
        <property type="entry name" value="CA109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038985">
        <property type="entry name" value="OPRN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37904">
        <property type="entry name" value="OS10G0566900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37904:SF2">
        <property type="entry name" value="OS10G0566900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15011">
        <property type="entry name" value="CA109-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="199" mass="21293" checksum="89BDDA73EA785470" modified="2025-10-08" version="1">MDFSKFKLAVLRANDSIEKWSALQEAASKLLSNAGNILQRLPVLSDARNFAALRQARQLQQLVLAKQIRALEAVFGRLQSNLGELEGLVRIQERLVVEVWKLLGEAPSVEGCGTVQPGGASVAQLVESIEDVWRLCRDDLAVRAAALADLNYSTSPQQFAEIYEALKSCTALLPGGVSAGSGGACTAVVLLQSVAAAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2Z5</accession>
    <name>A0ABQ5S2Z5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007488</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="16"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="43611" checksum="FED6B77CD65EA37B" modified="2025-10-08" version="1" fragment="single">SFPGCLGAMSHRKFEHPRSGSLGFSPRKRCRRGRGKVKSFPRDDPSKPLHLTAFMGYKAGMTHIVREVEKPGSKLHKKETCEPVTIIECPPMVVVGVVGYVKTPRGLRSLNAVWAEHLSEEVKRRFYKNWYKAKKKAFTKYAKKYSDGKKAIDAELASLKKHCSVIRVLAHTQVKKLGFGQKKAHLIEIQVNGGTVANKVDFAYGMFERQVCVDAVFQPNEMIDTIAITKGHGVQGVVQRWGVTRLPRKTHRGLRKVACIGAWHPARVKWTVARAGQQGFHHRTEINKKVYKIGKKGDASHSATTDFDVTKKDITPMGGFAHYGIVNEDYIMVKGAVPGTKKRPITLRRSLLPQTSRNALEEIKLKFIDTASKFGHGRFQTTEEKIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2Z9</accession>
    <name>A0ABQ5S2Z9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">T-complex protein 1 subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_007411</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03338">
        <property type="entry name" value="TCP1_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012717">
        <property type="entry name" value="Chap_CCT_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02342">
        <property type="entry name" value="chap_CCT_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI64216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="526" mass="57103" checksum="B5DEB9E548354AC5" modified="2025-10-08" version="1">MPPKQKNELYKESARKKDVRTSNIEAAKAVADAVRTSLGPRGMDKMVAAPNGEVIITNDGATILQKMTVTQPAAKMLVELSKSQDVVAGDGTTSVVVICGALLKKCQELLEKGVHPTIISDSFNKASAKACEILESIAIPVDMEDREALIRAANTSLSSKVVSQYSSLLSPMAVDAVLRVMDPARPNMLDLRDIKVVAKAGGTIDDSEMVDGMVFDQKAAKSAGGPTRMEDAKVGLIQFQISPPKTDIENNVVVSDYNQMDRIYKEERNYILSVVKKIKASGCNVLLIQKSILRDAVTDLALHYLAKAKIMVVRDIERDEIELISKTLGCLPIAHVDNMKPEKLGSAKLVEEVEVGKGRVVKITGIENKGRTATVLLRGSNKMVLEEADRSLHDALCVIRCLVNKRFLIAGGSSPEVQVSMQLAHWSKTLQGMESFCVRAFAEALEVVPYTLSENAGLNPINIVTELRKLHAAGEKYAGINVKKGCITNMLEGDVVQPLLVTTSAITLATECVRMILKIDDIVPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S401</accession>
    <name>A0ABQ5S401_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045316098" evidence="2">
        <location>
            <begin position="25"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="229" mass="24098" checksum="4ACEFB577E232CAD" modified="2025-10-08" version="1">MVSPRAGLVGLVVLVGLLALKVDAGSLECTALLYLKGNYDGESLQLSLRPLGETDKNVVLDLASLPKPSTYRTWDDALNSARFSCNCTSENCDQDYSKMTLRIFAEPYWRKNGGGDKIDVNCTSVAGKPLCTGFLQYMPKGWGSRASAVAILYNLPNYVLPKKAPGPDAGATPSTPTPAPGNTTQSNPSPSPAAKPSNAPPPPKLSPKPSPKPAPKPSPKPSPKPSPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S424</accession>
    <name>A0ABQ5S424_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007949</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="22957" checksum="E1BBD614CA9FD191" modified="2025-10-08" version="1">MAPPPPLITAAALEVPAPLPAALIPPPVDPPVVVAAAVATAVAAAARDPVAPPSAATWATSSCMSKSGRPSRRPTGGDEKPRGEQANGLRHARTPAAGRRADCNTLITANDHCYISTHRPTMYLRPSKRTSSTTSPLNPSWSVYRAETSHGSTTNPHWIDCMWNDPAMGHGAQVTLTMNVTSAPSPLDISPSMSVANVDFVAASSQPGGHRSWCGGDRCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4T6</accession>
    <name>A0ABQ5S4T6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zinc finger HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048371">
        <property type="entry name" value="ZNHIT3_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21373">
        <property type="entry name" value="ZNHIT3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger HIT" evidence="1">
        <location>
            <begin position="67"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="140" mass="14957" checksum="B0A77CAC951892A9" modified="2025-10-08" version="1">MSGAIQRLQARIYRTEDAGVVENSTGRAVAIAAHIAALEHAASAGNAQVVSVSEEENDYKLGRVQYQALADSEQLRAMLANPHLQVVLAKIDNATDREQMLAAQLENPDFQDFVSQVLKSLDPERVPATAATVAAVAMAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S553</accession>
    <name>A0ABQ5S553_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007854</name>
        <name evidence="3" type="ORF">VaNZ11_008003</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="2 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="23124" checksum="3F005E2357692F3F" modified="2025-10-08" version="1">MAGKVSITSNWQCRPCCSTKDCHRGRPDGNGCGTNATAGTSNTPATWRHQRGGDTWPIPAAKIEDLLLTKAVYAPRHKFFTGLNRPEEGYKSPAGRTALDLLKTVCQSVWRERAHLHGVAGEGAGAPTTMTTSQLQGASWPATSVSIRRPSRQETDGGDPRRGQRNGDWAGRNPATTLSVAAAIIDSGVFDVGGHSGSAAALADSGTAVVAVASIFTRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7A4</accession>
    <name>A0ABQ5S7A4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">glutamate synthase (NADH)</fullName>
            <ecNumber evidence="19">1.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">VaNZ11_009430</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>2 L-glutamate + NAD(+) = L-glutamine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Energy metabolism; nitrogen metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Nitrogen metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.14" evidence="19"/>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00982">
        <property type="entry name" value="gltB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00713">
        <property type="entry name" value="GltS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012220">
        <property type="entry name" value="Glu_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006005">
        <property type="entry name" value="Glut_synth_ssu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051394">
        <property type="entry name" value="Glutamate_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01317">
        <property type="entry name" value="GOGAT_sm_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008730">
        <property type="entry name" value="PRK11750.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100:SF1">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000187">
        <property type="entry name" value="GOGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0288">FMN</keyword>
    <keyword evidence="17" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="13" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="22">
        <location>
            <begin position="104"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1059"/>
            <end position="1081"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="GLI65807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2308" mass="248017" checksum="A978D384B0B74A5D" modified="2025-10-08" version="1">MPHSLGSLALPGSQASFQSGNFIQQQPHLLIPGLLHRGGVNVAASNGRGLPRNNAATCRPRHTLLPRWIPVGPGLGSRLVLCDAHSAIPEPAGLFNPDNDRDACGVGFVGELSKRPSRKCIKDALMMLQRMTHRGACGCEANTGDGAGILVAMPDGFFQSILMEEQGLKLPPLGAYAVGQVFLPRDEALRQKVKDIAEKVANQLGHVSLTWRQVPTNNRSLGRSALNTEPIIEQWFITSTGNHAALEVEQQMFILRKLIEYNLRTAGIRDDDAYFCSLSSKTIVYKGQLTPEQVRVYFKDLQREDFRSYMALVHSRFSTNTFPSWARAQPMRLLGHNGEINTLRGNANWMRAREGVMACLKLGLPKEVLQQLEPIIPATSSDSGAFDSVLELLARAGGRDMPEAMMMLIPEAWQNDALMSKDKKAFYRYHSAVMEPWDGPALVSFTDGRYLGATLDRNGLRPGRYYLTKGGRVIMASEVGVVDVDPEEVVRKGRLMPGNILLVDFDEHRLVEDKELKERYANRRPYNLWLDEHTFTLQQLTASVPPDARIPKPLPGSYPLAPSTASANGNGVPTSAASNIVSFSPVGSSGGTATANFASEQDAGIQSLLKPLKVFGYTREGLELLMAPMARTGAEPLGSMGNDAALAALSRRARLPFEYFKQLFAQVTNPAIDPFREAVVTSLRCFIGPETDITAEPGPQHCGRLELPQPILDIAEMEALKSMDHPGGWRTKVLDSTFSVEEGPMGLPAALGRLCEEAEAAVKSGSYALLCLSDRAYSAARAPVPSLLAAGAVHHHLVQLKLRSNVGLLVDSGEPREVHQFCLLMGYGADAVCPYLAFESLAALQRDGKLPATVPLEELKAKFIKGVGIGILKTMAKMGISTLQSYKGAQIFEALGLAPEVVDVCFRGTPTRIGGSGWGQLGRDALILHASAFGVARLPEGSADAKALPNPGDYHWRNSADAEKHMNDPEAIAKLQAATAGNDRELFRQFSALNTRLSRACHLRGLLKFCTEAATPVPLEEVEPASAIVKRFVTGAMSYGSISLETHTTLALAMNTIGGKSNSGEGGENPRRLEPLPDGTKNPFRSAIKQVASGRFGVTAYYLTNADELQIKIAQGAKPGEGGELPGDKVKGDIAVTRGSTAGVGLISPPPHHDIYSIEDLAQLIYDLKSSNPSARVSVKLVSENGVGVVASGVVKGHADHVLISGHDGGTGAAKWSSIKHAGLPWELGLAEAHQTLVANDLRGRTVLQVDGQLRTGRDVAIACALGAEEFGFSTGPLITLGCIMMRKCHTNTCPVGVATQDPELRAKFAGEPEHVINYFFMVAEEVRELLASLGLKSMNELVGRADLLETDMSAIAEGGPKLEGINLSRLLLPAATLRPGAAQRCIQKQDHGLDSGLDVHLVPLCKAALPDEPDGSAEPVYIEMEVQNTHRAVGTTLSHEVTKRFGDVGLPDDTIHIKLTGHAGQSLGAWLCRGITLELEGDANDYVAKGLSGGVVAVYPPFNSTFRAEDNVIVGNVALYGAVRGECFVRGIAAERFCVRNSGARAVVEGVGDHACEYMTGGVAVILGPTGKNFGAGMSGGIAYVYDPNDKFKSLCNVDVANDLLPVESPEDVRTLKSLIQRHLKFTGSDVARRILLSWDRSRVQFKKVFPHEYRRAMAEAEALAKAEAAESALLAASDSAGLAPKDAFEELKAMAAAAVKAGKAPKALTLNVIPTADPQQDKRRLQKLAREGMPVKGIKPTWEANRPTIVTPKVLAATPAPVVAAAPAGPAADKVRGFVSYDRKPLPYRPEAERIKDWDEVHDHSGTAAHAALLHTQSARCMECGTPFCHTASTGCPLGNKIPEFNDLVHKGRWREALDRLLETNNFPEFTGRVCPAPCEGSCTLGIIEPPVSIKSIEATIIDKAFEEGWMTPRVPKLRTGRRVAVVGSGPAGLAAADQLNKMGHDVTVYERADRAGGLMMYGVPNMKAGKVDVVQRRVELMAAEGVKFVLNAHVGVNVPPQDLVSGHDAVVLAAGATKPRDLPVPGRDLTGVHFAMDFLTANTRSLLDSGLQDGKYISAEGKKVVVIGGGDTGTDCIATSLRHGATSIVNLELLDKPPTTRAKNNPWPQWPRIFRVDYGHAEATHVYGADPRTYNVMTKRFVGDEQGKLKGIEIVNVKMEKDPSTGQFRPVEQPGTSQILEADMVLLAMGFTGPEDRLASALGVKQDERSNFKATFGDFATSIPGVFAAGDCRRGQSLVVWAIREGRDAAAAVDKYLTKQSGNNLFAQPLEVQGGIFDVSGTVILRPSIHSLPRDASPKRPPVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7C3</accession>
    <name>A0ABQ5S7C3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="907"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="626"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="626"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="930"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="930" mass="94782" checksum="25F1200A4ACD6F66" modified="2025-10-08" version="1" fragment="single">MPPKRGKDIDIQKLKALEPCKLDGTLQFGDFPPRLCSVRALDSELCNSKRHTVFCDTISDRLAVQFPMGLAIGAAASGQPDLRIELVQLRWGNTGDTSDGDHQAHLGDLRTSPWGSYPRSWSTADFNQLVCPWDNCQCTTGATGGRDAQSFAASAVVARRTGDHLHCRRGGGGTNSISRCSSTAGALLLFSVLYRDTVAAVGPSLVLPGLAVLAGEMTAAVVTALAAAVPPPVPRAATDPDPCSCWHRLTAPDAGREGGLIPLPATARNWRRRLGAIKAGLGHVSRSAAASSLKRILGASALLSLAAKTWRVVHASSAALAEAVADLTAVGLSYSCLARQAQLGGLPPGRTREMAWRKLHKAAAQRLARHLGDLPPQPPLGPALAALQRCSIAAGLAVDGVPLCGAREALEAYGIGGLGSCESGGGGGSGGGRDSRQGRQLSLSFSYSHRRRQHPRRRLAAAAAATATRTNTTDEHLNLKAISLGVTGGGGSGGGGCGSGCGGGGGGCVVPNTATVDAIQLTPETLPSKQVAAAAAAAAAALNDSRGTCSRTAESMGRSPPPYRNPPGSGCSETTPPADQRSQSLAPLKPLHTPAARQSPPSSTTSAASELLDEVRRQTAARRLLGAAATAAQQQQRPLSTSRDGGDGNVSATCAVAPPVPTHLQPAAAIAAAAAATDGSAELCDSELSWGSAVSGSMQRHVAISPVVNQVASSSGAPPGVGVGVRTCPMGPVTPDSRRPVGPSILGTNMSGGQAGIRTGASNGLDCTDRAGGTEALTAAGPPPSAFLVPWDHTSPGANSDSQQPPTTGPVVAKAQIFPWRVADGSSVMGGGGGGSGGSGGGEGGGGCKWELPPLTREFEERMTRLEQEEVEALGLPLYGRTDHADVRQLQPRPMGPRERLSLGSRAVYLLLVFAPFLTLGVAMLLLAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7W5</accession>
    <name>A0ABQ5S7W5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PKD/REJ-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049804">
        <property type="entry name" value="Choice_anch_L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002859">
        <property type="entry name" value="PKD/REJ-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038133">
        <property type="entry name" value="choice_anch_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02010">
        <property type="entry name" value="REJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKD/REJ-like" evidence="2">
        <location>
            <begin position="352"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="726"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="727"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="904"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="938" mass="97938" checksum="EA57C4EB01B04D47" modified="2025-10-08" version="1" fragment="single">KSTTWLQIHAPIMARTPTMNTVVVSARNIFILAAAIFSLVPNASGTYDEITDLISSRRGHVARKSGSAFGMRRQLSAGCGPFIACPSAIDAAKIILGNNPNLTISNATFTKGTCTSTVSQWGIVPAGGWAPGHVMTAWFPNGSLVLSSGDASAGNCAVNTLDYFTLMVGGGGDANLNTLIPAYITYDAVALEFVVTAMKDGLLVFKYAFGSDEYTEWVGTAFNDVFGFFIAPVDQPITSGHNVAIVKGTTNTQVSINNVNFKSHSDIWTNNRIGAVNPLKALEADGYTNLLNTQGFQVTANQQYRFKLAVADAGDHVLDSWVWLGGDTLIIDTKPVANGTVDTPTATCAKKIITLNGSSSYDPDTNDVLTFKWVISASCYPTVTTTGAVITVDLSTLADNVTYTVSLFVTDSSDVEDVFITQLAVPPGCGSKAISKNCGSSPNPPPPPPPPNNKPSPPPSVIKSPPPPSPPPAPPPPPPPPPPYIGDYIMAGNAGVVPCGSPGVTIDVSVADDGIIAQINTALDGAPYTLYYIWAVFDMYENDIPVFNKTTNSPITSFTAEELLQGRSLKKYKVLVDVTNTDQWDQGDGGILSAETFIQVLQCPIQPSWPLPTIIFDSPESFTLDCGTSVLLDASAAVASAAAKKGSNGTTQMLRWTLLDDSEVVILTFNSPAPSTQLDGGALLKSGAINPGIFYTLRLVVFIDTTDFDSDSWETKISVLACSPGSYPSPPPPPLSSFKPPPAPPSPPPPKPPSPPPSPPPSPPPSPPPSPPPPKPPSPPPPSPPPPLPPKPPPPPPPLSTTSKPPPPKSPSPPPPKPPSPPPPKPPSPPPPKPPSPPPPKPPSPPPPKPPSPPPPLSTTSKPPSPLPPSTASKPSPPPSSKPAPPPPPAPKPPSPPNKPPPAVTKSSPPSPVIVKPSPKLESPPQPDSPELADRRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7X3</accession>
    <name>A0ABQ5S7X3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellar outer dynein arm light chain 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21459">
        <property type="entry name" value="DLC-like_TCTEX1D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF7">
        <property type="entry name" value="DYNEIN LIGHT CHAIN TCTEX-TYPE PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15959" checksum="FF98E8DE60A689C8" modified="2025-10-08" version="1" fragment="single">MDDSMPFQSGGIKEVVYENTYITDPEGYGPNTKFERHKVQAVLKQVLKERIEKQKYDPVKGAQISKQLSDDLREKVKALGYDRYKLVIQVTVGQKQGQAMRIISRCLWDTQSDNFASEFYENESMYCVCQVYGLYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8X0</accession>
    <name>A0ABQ5S8X0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="433" mass="43895" checksum="B422E57BC59A3F95" modified="2025-10-08" version="1" fragment="single">ATAATEISEAAAAPLRGGRPPPTPQPPSSSPLPVTPLSGVRRASFSAGFDSTIVVKEDYGSTVFVHEGFNTVVVQSDGGGVGGASSHPALAAAFRSPAPSAAAAAADYGATTVVGATVVERTETVRRSSDGAGGSADGGYMAAVRAAAVEVQHGDRHARPAESPAAGGHQPTPQQNEVERVRERLQAIYDGGLVVPLPFFKASQAQPLALLRPSEPSAPGPLMPPPLPPPLAAAAAAPPPPPPSTATQPQHSTGAMTAVAAGAAAATAAAAGNCRGQMHNNGATNGGSVAAVPRPSQPTALAAAAALPPPLPSQQQQSSSIPQQRQQPNTAAAVDMHGVDPEAYGVVLELVQHSAAMACQRGEVASEQGLMIDTLPPTVLTQLQFHPALQNLARILAYNRRCLAVLPLERRAQEELQESCNQLSAALQCILSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S992</accession>
    <name>A0ABQ5S992_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="149" mass="16198" checksum="73225A9E7755855D" modified="2025-10-08" version="1">MLAVLAVCSGRSCSHPFQVLHTDGSVSDLWDAMDEQYDDMYDAIPAFSFGRCLPYQDLENEWSSFRGAVVPDCAENGSAWSAPRRVFFADVGPHAGSREAFRHGNSDAGDVFVKVESELESAAVPLPAKYQMVTDVYGSNAARDAILLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9W2</accession>
    <name>A0ABQ5S9W2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010461</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="227" mass="26051" checksum="0E4121141234AA5C" modified="2025-10-08" version="1" fragment="single">LSFGLTNAPATFQAVMDRMFRPFIDRFIVCYLDDILVYSKTREEHLEHLRLVLDVMRREQLFAKRAKCFWAQPQVEYLGHIVSATGVKMDPRKVAVVRDWPAPQNLTELRKFLGLTNYFRKFIEKYSIIAAPLTNLTRKGVFVSLESWTSQCQRAFEELKRIIAEDVTLAFPDYSLPFRVEVVSDASIFGSGAVLLQEGRPIAFTSKKFSGAEIRYTTGEQELLAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAH4</accession>
    <name>A0ABQ5SAH4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="146" mass="16317" checksum="051B615687DFA3BA" modified="2025-10-08" version="1" fragment="single">WAGDTNSRRSTTGYVFRVNGTAISWSSQLQRTVAASSVEAEYQALSSAVREVLWLNKLCGDLKADSDGIRINIDSQGALDLGNNPITSSRSKHIDVQHHMIRERVIEGDVALRHCRTEEMVADALTKPLPEGKFVWYRREMGLDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAS8</accession>
    <name>A0ABQ5SAS8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_010672</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000039">
        <property type="protein sequence ID" value="GLI66731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="6">
        <location>
            <begin position="165"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="88"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="655"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="753"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="803"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="864"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1032"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="516"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="957"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="381"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1042"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="249"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1106" mass="115116" checksum="4FE86F9142EB6F4F" modified="2025-10-08" version="1">MSQQGNPSPVGILSRIPSLSTVRSCANSRLPSPPVSPGISSPCTPQPGLNLTGNSNAQWTAGGFNPAQDKLVSSSRGRFFPTVPALASPTAASAGGHPPAGISRSSSMRASAPNFARIPLSPHGASMSGSSMPLGTQEVAPPPSSHAYGLGELDSPKGLASSRSTIQVAVRVRPLCEKEKQRGDRVAWEADREGNVGMVDSATGAFVPKHRFDTVFGPESDNSAVAAKVALPLVAPALRGVNGTIFAYGVTSSGKTHTMMGTDVDPGVVPRVLRELFSQIASAKMSALPGLLSYTVRISVMEIYNEVLNDLLEPARANLKVREDSRSGLVMVEGLLEQVVITADQALELIARGDHNRKVSATAFNEDSSRSHTITRIIVESGMSSPGPSDPPASSNDRAVGRRTTACLSLIDLAGSESARAVVSKGQRMEGSFINRSLLTLGTVIHKLASGATGHVPFRDSKLTRLLQSSLSGPGARVAVVCNITPAAAQTDETANTLKFAARAKLIQVTARTNEILDERSMLRHYQKEVADLKRQLAEAKRLLASSGITGADGIGPPNLIAPMSAGGQDERLNATVAVLPAMGTSEEAAAAALQAERDARRTVEIECTMARIKLTRLQAFLEENGVDVDNLLLASTSGELGEGGRNVLRPFTSSGEVSTSLPGGTPCPPGGDTVSTSPLFRRPLSLTDPPFGSGPLPDAHTSRLDGSLCGTRQKSNSGGAEVFDKSRVAYLNCSLGDAALADAAVENGRWPAWDSDAQRSGPHMAGSTTSSPSAFGGRGRPANESSADLRAQVAAALEVATGGSCDRHGSVSGNASGSDLEVDEDLNEGIMAQIRDMISEAQLSCPAPQSVSSQPVNTRVADGSIVEAPGGGRGGPSSPGRVTPSADAAVDANIGPVGAPSGLGFGAGGPASVWSQHRRSATLSTTSSSFGAGQELDMELELQVLNADREVLHDQLVASEAANEQLTAQVVDLRRQLATYEQLTRHASSELAEIRRLKDAFQARLQRENDALRLQLTQLGLTPAAVTDLQSQTRIPKGPSVQPSEPNRQAVSEAPRLATQSLPQLQPTTQAQNCVPPNMDAFGSPAAVAPSLLSRPAMLDIVGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC26</accession>
    <name>A0ABQ5SC26_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI66994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="550"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="714"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="800"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="550"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="742"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="875" mass="87980" checksum="BB82CBC5D5F3F536" modified="2025-10-08" version="1">MEAVGHEHRQSQDPQCLLAQLASEYERSRRFLEQLENSFAKEPQSLQPAVPTYTPAWAEESLEQPELPAAPEPVPLQTGTKQPPKSVPSKLRAKLRSFFSRRNTHRDECGEQTVIRASSESQSAINVSHGVGGSGCASSVSERPSIAGGGPPTSAAARRTHQMYYATSSAEMPRVSRAVRDPDTRPNTATRRSSIVYFSGNTMDSTSQGWVARVAVGGTGGGDAPVDRPATGRRSSIVQYGPFAAAERPATARKAAVVQLNEAAAALGCGHVTSSGQQSSTDPADQTIFGEGDSVAAATAAAAAASVTTARRGVPFVPFGGVSGGGGGGGALQDVCNPIEFMSVMAGPRRTTSFQHRPGTALQPTAQRLRSSASVVRRTSIEVCAQLLPQQQQPQQQPAQPPQSPQQNPFQRGGGRGSLEGRQIWFTALSTSAEQLPSSSRRAGSLEISRPYSAATVAAARLHPAAAATGAAATAATATAAAPVNISSSALFSSTPLVGATGNAAALAATGGGGGGGGGGGLRCEVPSSPQSSAFRLRLQTSPAGVAWAQPLAAPSPSNSPGSAGSPSPAEATTGSTIGRARAATAVGFGGTGGGASPVMRMASSFSARRLKDHVDGIPMASTATVTTTSVAAAAAALPLPPRHCSLSPISGGYVTNASADETQTATGAMVSERAPLAYGSAVPHVIVNSTDETVVVASTAVATAVAMATAAVSPSSSSSPNEPHGSQKMLQPVVFHGGGPSLQPLQPPQESSSQQQQSPPSVPHMPHPPPHPCAHLPQGHAGFRSRGFGGLVTAEEALGGAGSTSGSGSGRGGGGGGDANSGSGCGGGSTIFPTAAVGGFGEGRVTRPDSPFAVCMDERVGAAVAYEGAVHSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCM1</accession>
    <name>A0ABQ5SCM1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="40725" checksum="93846FC6C7DDF8D5" modified="2025-10-08" version="1">MEDFKTKINAVPYFRTVCLLKSCASLLRMPPRSLATALAYLHSWHRAANAGDFEDDAMLAACLLLASKVEEAATSNNHLLNVVKIMSQLQRDCLDPKDYRVGPMFQGQEVQLHRQEAEEEAEGAALRSRLHGQRQAQEQPAGDRCRGWGSGARIGSSAGAHPAPGRQIEGGTGGSSPDATAAAAAASLLLLQPDAAPPDALRACVLNCEVLVGSAYYAAKQAMLDAEQRLLRALQFRVRVVQPHALLLNAARCLRMPSGVQRLALAILNDLWAYTDFCMLLVPSPSSPSHDRNGGMTSGGMEATMAVLEAATRLSGWQVWVPTRARDMGLQWWQLLGLARDDAAMANLVTVVMQACADCHQATAATSKASKQGELTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDN2</accession>
    <name>A0ABQ5SDN2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAC-A/B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May promote appropriate targeting of ribosome-nascent polypeptide complexes.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22054">
        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14358">
        <property type="entry name" value="UBA_NAC_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016641">
        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015901">
        <property type="entry name" value="NAC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAC-A/B" evidence="3">
        <location>
            <begin position="47"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="190" mass="20685" checksum="B7EA5B43BC5300A4" modified="2025-10-08" version="1">MTKEEFEAKEGVEEVEDDDEDELSEDEEEADLDEGDEEGGGARGKQSRSEKKSRKAMQKLGMKPVQGVTRVTIKKSKNILFVIQSPDVFKSPASDTYIIFGEAKIEDLSAQSQAAAAEQFKMRPEPEMAQAHGAAAGPAEDDDEEVDDSGVEQKDIELVMTQASVSRAKAIKALKNAEGDIVSAIMELTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE70</accession>
    <name>A0ABQ5SE70_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="494" mass="52399" checksum="9CCB1ABA5AF0BB67" modified="2025-10-08" version="1">MIRSGLQSSINRVGATYGRSSIHRSSAIALGKEIRRSNRTRAQERSDQGAQTSGATSKRSAGTAEGAPSPAINGIPSNINTARDVPTLGINSTSFESSKLSISNNRKEKASSSSSGNKKEGDGDEIAGFSMVDIATTTSFIEKLAGALVMYGLTVLLSNLSDVDATHGMLWNDGVPLAIGAAAAAPVMLAVGLLFLPSLDVGFKLSELRTVSEVDQDEDAAILDLTGGLTGNMLGPDDEFRKGKEPAITSEAIKRGLWLYQVHVLKPWFPSFRWSSVGFLYLASCAAAGAQELLVRGYGGTLLTDWYNNLLASTTDVNNPIYWAKVFKLVTPDTGRWLAAFSLIALQVSLSGAAASRASSFARTALNRVGRVPSEPLVKGVVVQNVRLTLELGGKAEPMVEGEDKVTVEVPTPEDVVVYWASLGVSILLCGAVNCAWAASGSLLGSYTAQVVIDGVVTGMQQFKTTQLTEKELWEQIQQEQAEEDESDSDTAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEB9</accession>
    <name>A0ABQ5SEB9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36042">
        <property type="entry name" value="OS05G0490900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36042:SF1">
        <property type="entry name" value="OS05G0490900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18066" checksum="8B9C47CA2B70F862" modified="2025-10-08" version="1">MLRVVTASRHCCLSKSAMNTSIAPLAIGKRVTIFPCKQLSVIARVEPEQQSPPKPATKDEELPPWIRREKERELQAQQGASGLPWPLYLLFSIFTAIAAVGSIFEFVDRNPIFGVLPPDNPLWAPILLFFAVTGFPTAGILFVKGVNGFNEEAERQDKLDGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEH8</accession>
    <name>A0ABQ5SEH8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dual specificity protein phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012524</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015275">
        <property type="entry name" value="Actin-fragmin_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035010">
        <property type="entry name" value="PHS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47100">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47100:SF5">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09192">
        <property type="entry name" value="Act-Frag_cataly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="1575"/>
            <end position="1720"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="1641"/>
            <end position="1699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="761"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="829"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="894"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1093"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1188"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1258"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1331"/>
            <end position="1436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1758"/>
            <end position="1784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1834"/>
            <end position="1860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="588"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="639"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="665"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="906"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1126"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1188"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1264"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1361"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1420"/>
            <end position="1436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1913" mass="196244" checksum="2482FA278EBD39AC" modified="2025-10-08" version="1">MASDKSERWQVPLVSLGNTLSEARELAAVLEETLGVRIDYGTIILRSAATPQLRGHTFKERLVAGIPIPIDDRSINWSLLRGVVSTESLKDGEAAPGVHPGHVQLEVNSGGRVFFCFFAPTRAGEPEGVTVLKFGPNRLLMQAEQFANELTRHLDICAPDCRIVRQAGPSSEEWKALTEACVRLGGGGSAAELASELAQMPVALLMEYCPGRPLLQCEEAFLLDGPSRRTRGSTFEELGRLFLLDAVLGNADRLPCGELCWRGNPNNVLFGRAGSRCASHIVAIDSCVQRRPPAARVCAEDAGVDRLAQLLLTDQDFTLELLRGSVLAQSPPGLAALSATPAAATDAFRRGFRDCLRLVERIKGLLEMMYSKMDDWSMEFIKDIKELRPELPLPNPAPPGLASGPNSVTSGGSRVVAHYAGLNIRVNPAPGGSASGCGGSSGGGRNRSQFGCWGGSGAASNNNSGMGSPVRGRYSPSPYLTSSLRGTSRSRNNSLGGGSGGGGLPDGIGSGPSSLLRRRGSLAEAVAAAAAARAATAACAVATGAAAAATIVAAGGSDGIHGATRLGGDGCVATASAPSSDNDHAEKGAAGFGASKSSGGGAGRVVSPFAARNAEGPSRGNGSGASPNSLRNAPRRHQPLQQRPLQHQHSLRQHQQRSDGAGANWSHNNVGGNQAHANSQNFRLAPPPPLQRQRQQHRMKCRGSGLSPEVTAAVAEMPEWLACSPYLVSGPVVGANSGLAATVGRWRALTPGGGRRCVSVSVSSRSASVSRENTTSTSTTSSSNSCTISNSCSNANSGASAGLVRIATTPQSRLAPPVVASAGHETAAAAAQSLSPNPKLPRLARMSSPAGTYDGNDAVATPAAVQAVRRVTDVGSGCESAAAHVAMTPRKLTVRTTPSDVETGPRSGGTGDGDGGGGRGDGASVGRSMGSSPLQPLLTRIRTIGSGGGICTSTDSLNCTTPGLRHSRSFGLLRSPRYAVHNSNSPRRMLSPATPRFRSPILEPLPLASLAPLGSAPLPQDGASGTPQGAYCSDGGGAAITVSGAAAVGSIQAWMPKAATAAVQSPTLLQHCPNRQHTSDAPPATASVLATGLPAAAPKPPLPRAGSGRTSLGSAPAHGDGGGSSGVAAASSPAAGQSSPQQEELYRPQRREESPLGLAARALHSQGSPTGAPYTTTTLSCGGGSGGGGGGGGGGGGAAAAPGLGRGGGGRQEPGFKTSHESPPLARCIAGVVVTPTSTSTSTSTGSSPLGHFFRMRSQEHEGQQQQQQQQQQEFQAHGLQKRRQLHGSTVASPSGDGASLEPVTPFCFHTSSAMQVTSPQTLSVAFAAEQPVSRSGMSPTPKKVYPAVASTTPSPPSRPSPLSRTPPSASATPCTPTAGGASAGGASALTAAATPVSTSPASGASVRSTAANAALRRNSGGGRGRAGGVSGGSGCGGGTCVASRNLYRAASRDIPGSLNRGIGARGSPAKGNPYDQLKSEMSLTLALKSITSEARKDDLLSNRVQHWKELFRTRGEELKAAVGEWQSRHRLDKVLTTGFLDGTHPIVDTYELKVRLEHMLRRLQVLQEAAGAEKPSEVLPAPGPLFLGGAVAADSHHILRHLGVTHIVNSTEELLLPDPSAGFVVLRIALRDVDDEDISRHFGDVCDFIQSCHASGGSVLVHCSEGKSRSVTLVLAYLMRVQGWRLKAAFDHVKSCRPVACPNAGFMSKLLLYERDLFGDNSYISQQLKKAKPDPVVCPVCGAAVGISSASLAVHRKRAHPDTAQPQGQARQAQVREATATAAQLTSSNVDGAAAATAAADAHPHALRVGDGLRVAVATAGCGLKRANAAALSAPGSMPREYDVQQQQQQQQEGDSTGLSDCVLGVVQGDDMQEEVEAAECELCEGRKSAEGYVVASDAAATAPARLFSVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFD0</accession>
    <name>A0ABQ5SFD0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HIT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013024</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23024">
        <property type="entry name" value="zf-HIT_ZNHIT2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039646">
        <property type="entry name" value="ZNHIT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15555">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN CONTAINING PROTEIN 2 PROTEIN FON -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15555:SF0">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="3">
        <location>
            <begin position="10"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="99"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="552" mass="58450" checksum="B8311B9F6774246A" modified="2025-10-08" version="1" fragment="single">MDPADRQIICKVCLRQYARYTCPRCNIGYCSLPCYKQHGERCTESFYKEQAEGELRNVRAGDEQRRVMMDILRRYQQQQLLEEERQQLLLAEMRGLHVAEEWGGDSDEGEEGEEGEEGEGEAGLGVWGTGKEDGGGGGGGGWGPLASVLSEETRERLLRKATDSGGEDKWEVEDGDLTPDEWRAFQRAVALGQLCGSVEPWRPWWTSAEARELALTATGQRRITVLPAATGAAGDGAEGSDGDGPGGSGGLLPPPPVERLPPLSSLTRVAPSPLLTWHLVELLYSYCHVMRRFNGEWVKEPGAAAAVLLSLTDVLAAVPTPRARRHARSRSGSAAAVSSSSSTAAAAVTAVAAGATVSSPGSAEGDAEDGEVAARLPRTAVEACMHGMERACIPPIGTSKDRPYAVSVLYDVATLLDNGRSSVLLALEDARRLMVATAAGVDAAIPAANDTTASSVTTDGAVDCKGPQLCQPVKVVAAAGGGCSKGPGGRDGPAMMPVPSKAERVRLTAAERKLWFFLVWANELSEEQYEDLAGIVSAEWQLLHATVVAGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFD9</accession>
    <name>A0ABQ5SFD9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012237</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="107"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="140"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22228" checksum="CFD2E48793A8A4C9" modified="2025-10-08" version="1">MASSIARPLLTVSNKSIFSVWGSAQPQDGPTPPKFDEAGRLPAGLAAYRGAVDDLDDLVLNEDKMHAEAVDAHLETPLHIAATWGQLGIVRLLVRDHAVNVDPVNWEGWTPFFCAISRSHIEVADFLLSNGANLYHTTPSGMTAMHIAAWFAQRDSIEYLIQKRAPLHTENVYGVTPIGMTRPGPIKRMMFNALEEADKDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFE9</accession>
    <name>A0ABQ5SFE9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="260" mass="27685" checksum="0CEA693A468D993C" modified="2025-10-08" version="1" fragment="single">LGGWLDDWLRSRGSSLLLEIRRFSPIGRICSLKGLVTAMPKEATCAQLWICGAASPQGPSSMNDEVSTAALERTPLSVANPAAGSEDAKPMEAATATCRSWHFLIDGALSYGCLSIRDAPDAHFKFHAASVLCFCASKVLDSWQQQQQLGQRAQDLAENVTEGRLGGMGETVQQLEKDKSGVDSAGAGHDGDGGGGDDASFQLEIEAPKQQEDTSFVKGQHVQGQQPPPALQPALLRPDTLEILMGLMWANLDEPLAQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG14</accession>
    <name>A0ABQ5SG14_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="519"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="625"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="711"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="749"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="907"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="625"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="642"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="714"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="749"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="767"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="974" mass="99196" checksum="31A7ED30ED07342F" modified="2025-10-08" version="1" fragment="single">HMSHHHQDALLPPSVPSPEQSVLSTAAAATTTATATTPAPQPWPGFEPPRPLLPPLLPYQHPHSHRDPLDSGRLQQQQQQHLFPSGDVAVEAGAAPPAGFAGLAGAGGFGSALAAIPTAGWAEPIRQLYALLDYVAMQEAMVSGRLTTLRVHWINRPPALAHQAVEVVTVPQDCTLGYLKRLICKASGGAVWPRTVQPMRQDPSQGPAAPLHQEGASVPTQGPCVHSVAAPLQPLSAAERPFACSHLHGVRSGDADAGGGGTDEFNNSGAAANSARPATAAGSGQPAQQTAAFLTESLLPLNPDCGDIQGRWDPAVPAPSVPPQCQGVGVTGGDHQGGRVNAADSGITGDGSTGQGGGGDATGQGAGAGEGGSGDSVEGDASTLAEAGILPDSNVVMEADLQLPPSHQQLLMALQSQVMAAAAAVSTGLPGFHNPLQGSLPTAAAAAAAAAVAVTASVVAAANQPSSSTGLMGLTAEAGGDGEGDGADGHGGREFRGTHKDGAGSSRSRLPRGNYGGTLKERFTLQEMSALVSGIEEFGLKWALIKKSRKELQNKNQGDLKDKWRNWQRNVAVSWLTSRVTLPDQLRGRINALVAAAQRGELPTHTTPNLHVLQQRVCGMTAFQQHHSNPQHELQQQPPPQQQQQQQQQQQSGPEALSLQASTALAAVAAAAAAAAAMATGRPSTAGAAPMPADEAASRGLEMPVPTLDAEAVQGQGQARPSQQQPQQQEQMISITSVAPAARCGRQQQFSLQAGAAQSGASGGHQVSDHPHQHQHQGQEEGKGEDEAARAAQRQLQLQEAQAAQIAKAAQEAQAQAQAQLAAAAAAVAAAAAGQVDGRCGADMGLHARIQLAPHGSGHTADALRCIGLPLALPLSLAVPSSDEVPRLLLPAPAAAVAGIPVPGGPPGNASTHVEGGSGAAIEPGGDVQQSHEPAHQGYNLAAAMEDITTRGSVPEALVSQLGLGHGHLGPLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGJ2</accession>
    <name>A0ABQ5SGJ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_013441</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF53">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="5">
        <location>
            <begin position="62"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="6">
        <location>
            <begin position="164"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI68894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="38516" checksum="CC17D1C5C758CB0E" modified="2025-10-08" version="1" fragment="single">ETAPSAVAAPNIAQSELTSANYYFDSYSHFGIHEEMLKDSVRTRTYMNAIMNNAFMFKDKVVLDIGCGTGILSLFSAKAGAKHVYGIECSTIADQAKQIVKDNHYEDRVTIIKGKVEEVTLPVDKVDIIVSEWMGYFLFYESMLDTVLYARDKWLVPGGVIMPDKATLSLCAIEDGDYKRDKIEFWDNVYGFDMSCIRQLAIAEPLVDIVEPDQIATSIQTVVSVDISTMKKEDATFSAPFSLTLARNDYLHALVGFFDVSFTRGHKQLGFSTSPRARATHWKQTVFYLEDTLIACKDETIKGVLHCKPNIKNPRDLDISIDYEFKGERGQAKNSQHYRMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGS2</accession>
    <name>A0ABQ5SGS2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="567"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1161" mass="120700" checksum="145EEC23FE616687" modified="2025-10-08" version="1">MKLPSCLAGCFGGCARSHSETESSHFSVGKDPGPSCTVLSGFLNSFMSRRSCSRGTWDYGGTDTQKPHARARGDGDVVTLATARYIELFAASPKNDETSTVLSEMRGAQDSIRSARTASEIQVSRSSTFTGRASREPSRLAGNNQPQLTPLASLPASSSQRVPTLCHRRGASDALVTSISSGKDWPPFPPQAQPGLAPPQHSRRTGSMPHVSSQSPFTNKTRMPMLDNSRARSDRMSPSTAAYLLPAPSARAISRAHTALPIIAARSGAVPSSTSLNDLSQAPVESSFGDSGGLISATVRPQDPPSAAATTASGGRGRRPRLQTESGSTAACAPTMPASPPSPLDLASAAQPWFQGQNQFTMSSTPAQRTGTVRRPLPRRPSILFTRDGDVTVTGLPPSCLTAGRAPRGRLDMPGSMICTGAGSTAVIDASAPILCSALFRGGGSVDSASSQTGGLGGGVAFSGSRNSCVHEPITFTMLSRSIDRSTSRCTNASNVGLSVSNLRQHHRRRVGVGGGKALPGAGVSMSCGTNTVFMTAEGILHDCPTGGCTMEAQLGGFLEEEEKQPRQQLQKQQQGQQQQQQNSKGIQKLQEQQQDEGKREQKRSEQGPSPGGLNQGPQEDNNCQDGAHVNASQALAIANSSSEGRQRCDWGGSSMYAPSRFSDSSRLTHGNMTGAVTVTASMHSNNPLYTGNYRRILEPCTVGGTTADAFSSPAGTPGCLTSAVFSMTAGLAPAAPLVGADAAGSSPPVPPQAEPMKPYGADVGQSLLATEARDVTSALNRLQEACLAAVAQGDMRSQRLWLRTSTEVPRTGVDPSQRLARIGGGDRGSNMALIRMAPSLSFAAAGTAGTATARASRTAGIRTGPCCGPSTSLISLVPESSPRASPAAQDCGDDHMSRRVTQAGSVNVSVTLAAALRLKGEDPVGVNFTDCPEAGGFWCNAGFGVERTSPDGEGIVLDGEILRKRFSTAPDGDEGRDGDIAEGDGGCGRYIVQPCPLFNDTHGFSSEIEGLQADGSMGIYNDVGNEYRNRVLNVLLRSEFLYETHDAASDDDDDDEEEGVLEYRYGGFELGGGDHECGFTRQASFMEAVRKCGIPKAAVCAVGLDASQPGVPVSEVASMFQSATLAAVATGSRTGPSTAAALHRKGSCLAPAAAAHPGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHI3</accession>
    <name>A0ABQ5SHI3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CNNM transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_013338</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="11">
        <location>
            <begin position="44"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="246"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="482"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="561"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="502"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="597"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="619"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI68918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="700" mass="75081" checksum="A7C6A0A603BC2ADF" modified="2025-10-08" version="1" fragment="single">MRCGSADPQDCNYRMEIFMQLRNANIYQTIVQINDRLKAWTDNDVYEDWLYVIVCLLLTCMAGLMSGLTLGLLSLDTVDLEVLLRSGTASEQKRARKIMPIISNSHLLLVTLLLCNALAMEALPLFLDKLADPVTAVIVSVTAVLFFGEIIPQSVCSRYGLAVGASLAPLVRLLMWLCSPVAWPMGKLLDLVIGADHHTIFRRRQLKELVSIHAEDAGMGGALTRDEIKVITGALDLTSKVAYRAMTPLDKVFMLSSSDRLDESTLRSILRSGHSRIPVHREGDRGDVVGLVLVKELLQYRLGSSEDVPVSMLRIRSIPRLPATTRMYDMLRLFQTGRSHIAVLTQPPPGELRRLMALDPSLGTKRNDMSRARSDAKDRSEWVNAEGDTVAAIRRCSVAGEGASTAGNELAAAGKLATAAGSTLNDVRSVAAETEGDAENYFPYSLYHTASGGIDISYPPSAQDPQESSETTATVATLEAPASFRHWSIGGPETPGREDSDANTSGVIQQQGGTPSDLSDMRALRNRSAFAAPPALPIAAEVAAAEATSGAVLSFHNSPATASSMGPDVESGGTAAEDGVLRRPDANVADIGLGSWTDDPATTGTGAGLGSSDLPSTARRRDDTAAAYDRDEPEEGRPIGIITIEDVIEELIRAEIVDETDRYIDNNRLIRANQAQLAQSLPEHLAKVLAAAGASKKAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHY8</accession>
    <name>A0ABQ5SHY8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005527">
        <property type="entry name" value="MinE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03776">
        <property type="entry name" value="MinE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="409" mass="44339" checksum="DCA64282EACD4B00" modified="2025-10-08" version="1">MGSQLPRGQPSSPRLPKVMPQQPAAPALNLFTQGRAFRASRRPRRVIINAFLFKLPSSSGGKGTNRSRESIVNRLSELRSRPDDDTRNPSHPHPQPRPEDDIRTDAQLNSEAMPGERPGPPAQRQLNPWPAQTSGGDEEGENLVQGMAMDGSASDGSLTPRSLRYLISDPKPEIKLLHRFKPGQGPRTERAFWRSFTDKCRLAWDVFFPPEPTAIARRKSAANAMSTWGSGGGSFGRIFQSRSKQFGGGGRSSGDGTVVNGLTAKQVVLSRLQMVLIADRCGVAPEHLLEMKSHTLNALAEYMGKDLDDDMSQLEVQVSALKPNGERLTMTIGFADMLGEEQLRDPIEYHYEQFEDDDDYFTPEEDGEGVADIAEAPTAGLKAARVRATPVPGGGEGAAAKGGGQGEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIH3</accession>
    <name>A0ABQ5SIH3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013719</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045159">
        <property type="entry name" value="DCAF7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19919">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="271"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="369" mass="40620" checksum="1253E30CFA20AC05" modified="2025-10-08" version="1">MSNSDKRAEIYTYVAQDPVYAMNWSVRRDRRFRLAVGSFREDVTNYVEIISLDDDAGELRSDPALRFPHDYPATKLMWLPDREGCRPDLLATTGEALRIWKVMDTDSSTGEAEDVAAGGGTSTTGRGVMLRALLNNNRQSEFSAPLTSFDWNEADPKRLGTSSIDTTCTIWDIEKGEVDTQLIAHDREVYDIAWGGLGVFATVSADGSVRVFDLRDKEHSTIIYESPQPDTPLLRLGWNRQDPRYMATILMDSPKVVILDIRYPTLPVAELHRHQAPVNTLAWAPHSAQHICTAGDDSQALIWDVSAVGGGSGLGGQHRAAGGGTAGDVSLDPILAYGAQSEVNQLQWSSAQPDWVAICFANKTQILRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIM1</accession>
    <name>A0ABQ5SIM1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75011">
        <property type="entry name" value="3-carboxy-cis,cis-mucoante lactonizing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045750669" evidence="1">
        <location>
            <begin position="31"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="455" mass="49462" checksum="46EBDE60B89E7829" modified="2025-10-08" version="1">MVDMTMHFRAQRQFILLFLAMLFGVTFAGAADVDICNYELWIVDQADSNNGTALIGYGGYIHIFNNSNFKRPCDIVDLGAETAQLCFQRTGAYPVRPHMLFFNLAGSHAILAFVASGHVVIFRAADRKPLTCFRSTPGFNGLRQAHAAIPTRNNKHIIVANQNGKQVERINVDWEREEFVYDLNATLALYGNCTTPNGLPCESPDLRPDNAPICPPSLADPTLTFVTLRGGGMFVIDYTKTPLAIAAEYDKSTIGANGCGGVSTKHYMYINSGGGTASNLYGFKVYKLPTRGYSSANAPNVPAPKIIANDSTGDRDAHFAGILHNKRMNPTYYWQPDRAQSVIDVFSFNTDKFVNRIDLKATNLSTDPTPDALVISPTMAYAFVSLRGLIPLSADPHVSTGSSPGLGVLVVKNYGRTARLASISSITNIVNGTNRADPHGLDLRVTSDSCDVAYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIV8</accession>
    <name>A0ABQ5SIV8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="33987" checksum="34F7AB2B71218503" modified="2025-10-08" version="1" fragment="single">MSAALSSAHGGTATATVFAGEQLGQLSVGVTTCKDGQERAEAAKSSRWFGSCPHPPAISRPHHARRIHAAEMPHQRLTPYSPLSRWQFPRWHIPFYHPAILSLHLLLLLPLLLGWLPFSRGTTTCPSPVTNCTASDIVQYAINVSASPAPKDPLAANISALLGAPDYAGSRCLGGGAMTSVERQEVFSLSGGNSSDHSTTQLTLTFDVSVYMRQVGIYIVSAVQPNSTDSMAAILDDGKSTVVKVTCATDRTSSCLGKTASEQPFWVVCNGTPTIGPMKMIRLKVDDTVAIDAVYMVGYSASGSLPPPQPSPPSPPPPLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIX1</accession>
    <name>A0ABQ5SIX1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="19754" checksum="25C8A123AA35E7F7" modified="2025-10-08" version="1">MMLAVLRSTCTGLPGPRNTSGNPWKTRPIATLIRSVNYSSGHGSSQQQLKPVRIYAPNDDTGGQLEAEVLTSPGSHVFTVSLKKPLGFILTERGATIVVESVTPGGHAAAAGIVSGDVLLATSARAQSDTGKGALRGQHVLLSTAGEKFSTVAAAIRSNTCSQCNVHLVFGRPEANQLSDPSVVVADAGVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ19</accession>
    <name>A0ABQ5SJ19_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="332" mass="35685" checksum="701C21FDD05BA858" modified="2025-10-08" version="1">MSCEFIDVFFPSISKLLRSQYPRIWTHFFPPELQLKCARPTYDLNIGGEGRYVVPIHWPALALVIPVGVAIGLYGSLQLRVVKQWKPNRPPTAAWALAMGFYACMNIAALVSHCLVQRSSPWQQIFISMDIGATACTAFSALYASIGDELSGGFQTASANDGGNGSKGNPNLNLFLGNAILTISAFIGLDIPFMAELLYIGTTVLSAIVVGIYICRRPCCGRQRLFIWLVFTGGATALSGLPLDTTLCRELGPHVNHVTMLFVGCHMTLLGLFLYAQEELVVRHNMPAAAAMKQVVETAAGDTAKGEAAATGEMAGAKTAPGSEGASLRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ71</accession>
    <name>A0ABQ5SJ71_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plastid lipid-associated protein/fibrillin conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013822</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039633">
        <property type="entry name" value="PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006843">
        <property type="entry name" value="PAP/fibrillin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31906">
        <property type="entry name" value="PLASTID-LIPID-ASSOCIATED PROTEIN 4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04755">
        <property type="entry name" value="PAP_fibrillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0934">Plastid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastid lipid-associated protein/fibrillin conserved" evidence="3">
        <location>
            <begin position="58"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="263" mass="28960" checksum="D07BB90B36D6901C" modified="2025-10-08" version="1">MALASTSYNALAQCSRRYTLHYHVAAVRQHRATLSGRPARLRPAAFESVSLDDLLPTKLELLKKVACLNRGAIASSNDKYEVSSFVEVMEDAAGSLVQLDDVQGKWELLYSSVEPFRSSPFFWAFQEGLVQSRGVAAQIFSFTDSIPGAHVGAAYQTISFDTAKLISEVDLEVFPGIRGTVVTTSMVVQEPPRSLAVTVQNTRVANSNLLPFVDSVTVPVQEIMEAVRGPGSTRVAVDITYLDSDLRISRTRPDNEIFIYRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ94</accession>
    <name>A0ABQ5SJ94_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19094">
        <property type="entry name" value="AKR_Tas-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43147:SF2">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43147">
        <property type="entry name" value="PROTEIN TAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="88"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="447" mass="48019" checksum="063895FCA4114A65" modified="2025-10-08" version="1">MSISRYSRTNAATGNKTAMEIKTSGQSLTYTFNVCLRESLNLQRAAVSCSALGKTGGEHYMERKTLNKLHSVVGKRSLGRGGPQVPMLCFGCMLFGESTSYAEAEQLLGLCLDSGVNFFDTAEMYPIPQRAETQGASEEYLGRWLRSGGVRREGVLLASKVAGPSGQMTWLRGGPHKVDAANIQQALDGTLQRLGTDYVDLYQIHWPDRYVPMFGDSEYDPVYSYDGAVPLEEQLEAMGRAVRAGKVRYVGLSNETPWGLCKALAAASLDRSLPRVVSLQNAYSLTCRTFDSGLAEVCHLEGVGLMAYSPLAMGLLTGKYQAPGGGPPSARLNRYRGRYAEAESRYGPKSNVQEAVAAYCALAAEAGLSPVQMALRFVLSRPLVSSAVIGATNTDQLVELLAAAEPAWGSGTGSAVSEGLGGDAQWLPVEVLEAIDRIHTRLPNPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJH9</accession>
    <name>A0ABQ5SJH9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_014313</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048685">
        <property type="entry name" value="COG3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048320">
        <property type="entry name" value="COG3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007265">
        <property type="entry name" value="COG_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302:SF8">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20671">
        <property type="entry name" value="COG3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04136">
        <property type="entry name" value="COG3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 N-terminal" evidence="10">
        <location>
            <begin position="114"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 C-terminal" evidence="11">
        <location>
            <begin position="317"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="409"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="649"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="649"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="905" mass="96464" checksum="7479F97A8550AD78" modified="2025-10-08" version="1">MSTGSGVKGPNKPLGGVASKSYNVAAIWEKTAALSESQLRAIEGLGQFCSQRPLPSHVIDEQRSTVESAESHSKDTYVGTLEDAVLHNTSQFHKWHSELEAACASETEEKYKRYADLLSSHVASCENILAKVDLTLEAFETLQAQHRDVVSRSRSLHASCEQLVRQKEALVELAEAIRAKLRFFDEFEHVYAQFAAAQLSLDADQFMALLRKLDDCMAYVANNPQYADAQQYSAKFRQLQGRALTAVRGKVQQVLRAAVQQVQTAIQQAAAGVGAGTNGTATAATNGSSSVANGTSAPTVSGGGQVPQLAEGAEVPMLYVRFRAAAEPNLKGLLREVEARAGRPEYMRLLTECHSLYAQARLALIGPYVNHRIKQYGALALPLFTRNGCEHLSRVCQLEVQLFEHFFPGQQAQDSQPPPKTTTGLGAKSTAGTAAAPPRPVLPSSADALAPLLEPLATMLYDQLRPAVVIMQDQDELCELVDILKHEVLGEQLARRGIGGEALKPLLARCLADVQGRLIFRVQAYIRDKISGYVLSPEDTDYPAKLERQAAAAAAAGAAGTPLPASTSASTDGAGGNADRNGLEAAAIAVAAAGGTDPYATLFPPLRATLLVLSKLYRAVDPKIFGGLAQEAVSACTLAVQQASRAVARRASPPPSSSSAVNASGPSTGGVSGSSTTPLDAQLFMIRNLLFLREQIVPFDVDFAVTDIDLDFSHMRDHLRRIMLGQESLFTLGPSNAMVRMLGASGPRVFTYQLDSKKELEKVLKAVCEALIMALTKVAVEPMLSFITKVTAVRLAAAGQGASAAGGQASKPLREQAFASPGKLTEMVGRVNAALAGPLPGAVAKMRLYLPNPSTHAILLKPVKSNIAEAHGQIAKLLLTEYSPDEAAEVPLYNPQQLATLLDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJX6</accession>
    <name>A0ABQ5SJX6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014621</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the caleosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007736">
        <property type="entry name" value="Caleosin-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31495:SF0">
        <property type="entry name" value="BINDING PROTEIN CALEOSIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G13750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31495">
        <property type="entry name" value="PEROXYGENASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05042">
        <property type="entry name" value="Caleosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="54"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="157" mass="17556" checksum="8DC3799BFC91659B" modified="2025-10-08" version="1" fragment="single">TLAAVPVALAVQLPLSWLTGDSWLPDPRLLVHVRNAHKAVHGSNSRAWDRSGHFTPARFEAVLSKYDKDGKGGLTLWEVIRFLRGQANLGDLIGIMASAGEWLMTWALLRDSKGVLRREDMRGMYDGTAFYRLAERNGYKHYGMLTAREPAVMKGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK12</accession>
    <name>A0ABQ5SK12_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'(2'),5'-bisphosphate nucleotidase</fullName>
            <ecNumber evidence="3">3.1.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_014706</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3'-phosphoadenylyl sulfate + H2O = adenosine 5'-phosphosulfate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:77640"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine 2',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:77644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10041"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01517">
        <property type="entry name" value="PAP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006239">
        <property type="entry name" value="DPNP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051090">
        <property type="entry name" value="Inositol_monoP_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01330">
        <property type="entry name" value="bisphos_HAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200:SF6">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200">
        <property type="entry name" value="PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI69961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="401" mass="43640" checksum="1048640EDDBDDE44" modified="2025-10-08" version="1">MQNLLRQQSVHIRANYTGVTRLCCRRPFVRRRASVHVAVDVEPQTLQYGKELENAKAAVRLASKLCQIVQRQLSAEERADKKDDSPVTVADYGAQAVVAWVLQWADPASRLSMVAEEDSAELRQPEGRPMLERITQLINSVFAEAKVGVILAPEDVLKLIDLGGSQGGPTGRHWVLDPIDGTRGFVGMRQYAVCLGMLQDGEVVLGVLGCPNLPQGPVGEQDGQEGAAQRAGGSDVGCLFSAHRNHGAYAAPLWDEHADPVRIQVKDLSNPREARFMESVESRHSSHSTTAAMARELGVVLPPLRMDSQVKYGLLSRGCASIFMRFPPPTYKEKIWDHAAGFVIVEEAGGRVTDAGGARLDFSRGRFLQLDRGIIAAPPALHQQLLRAAAKVAVAPLPSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL74</accession>
    <name>A0ABQ5SL74_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome b5 domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_015156</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Radial spoke stalk protein that binds heme under oxidizing conditions. Required for the coordinated beating of multiple cilia maybe by functioning in a redox signaling pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052320">
        <property type="entry name" value="Cytochrome_b5_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21281">
        <property type="entry name" value="CYTOCHROME B5 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21281:SF0">
        <property type="entry name" value="CYTOCHROME B5 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="10">
        <location>
            <begin position="9"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI70376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="230" mass="26450" checksum="732D8D0DF220B55C" modified="2025-10-08" version="1">MAPPRGPLRRYYTPYEVAMHNTPDDLWVSFLGGVYNITDLVEEHQGTLAAPLIAAAGKDISHWFDPSTGDPKRHVCPVTHLERYYTPMGRFIHVPPPEPMSNWDTGFGQPWWKNAKRYQIGFLSARTRVVRIRNVLTDQEDQIEVPCEEKLVEIRERYLDLNAHARSYTWKAIIQDKDTGAHAFKELDLNLTLAENGVPDEGPTFEDHHVPGDYYIPVLHVYWNDDLTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLH8</accession>
    <name>A0ABQ5SLH8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="51386" checksum="957373F7357E2A74" modified="2025-10-08" version="1" fragment="single">MNSSNRTDVIKLPLPAGIKAGFVVGRGGHNCRWLHYNSRGAHITVNDTAISIRAKDNHVRARAIQLVRRQLEAMLRLAIYGCSNPVRLDYVLLSSAFGADPRVRFCSRKDEVAAFLGHKDSLFELVPNREDVSKFPAEKFEGILGPSKDCSFDASASSKTDTLKSDEHENTSSPHQLRRDQDGERDQDLARMWDRQQQPEDDTDGATRFHAATAVATSNACVAASTALATAAHVAKRSGSPPLLASAARALAAAAHKAACREPSFDVLKIRFKLGKSLFRNLGPRAGRTDLSVAELEGAGAGRGCFQSVFSTWVPSSAVPRVTSWLTDSLGFCLADTKTTATLHVINQELNIHYSIALAMERGDDSTASLRKVKAGGSKNPSVTLMAAPQQLDLRLKLISQFREHPALACDADAHSTARRVAAAVRMEGFEGFTRSKRGLPRNRHLEYARHKTKFVYEGYLPVRGQSRGSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLM0</accession>
    <name>A0ABQ5SLM0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">1,4-alpha-glucan branching enzyme</fullName>
            <ecNumber evidence="4">2.4.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_015916</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11321">
        <property type="entry name" value="AmyAc_bac_euk_BE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02854">
        <property type="entry name" value="E_set_GBE_euk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF2">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME, CHLOROPLASTIC_AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="8">
        <location>
            <begin position="258"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="747"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="750"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="806" mass="91698" checksum="C5F5ED007DF130C2" modified="2025-10-08" version="1">MLRSISKRSPGISHAPTVMKNRVIYDVPRSSYFVVLGGRNRRPIQRIVHRSALPGTANAAGRTVIKDDIGVLAVDPQLADYADHFRYRLQQYRAALNRIRAAAGSLEHFAEGYKIFGFTREDDAIVYREWSPAVQAAVLIGDFSGWQPVSMQRDEWGVWTVRLPDVDGHPAIPHGSRLKVRLQHPGGWWVDRVPAWVRWAVAEQRMGAGFDGVYWDPPKAEKHHWQHPRPPRPPSLRIYEAHVGMSSEEPKVASYTEFKDTVLPRIQALGYNAIQLMAIQEHAYYGSFGYHVTNPFAVSSRSGTPEELKALVDEAHRRGLLVLLDVVHSHISKNTEDGLAGFDFGQKEDDNYFKQGDAGYHRLWDSRCLNYANFETLRYLLSNLRWWIEEYHFDGFRFDGVTSMLYHHHGIHMSFSGSYHEYLGPATNVDAVVYLMLANQLVHDLLPQAVTIAEDVSGMPALCRPVSESGVGFDCRLNMSIPDKWIQLLKHTRDEHWRMHDIVTALCNRRYTESSIGYAESHDQALVGDQTVAFRLMGAEMYGGMSALIEPNEVVMRGIALHKMIRLVTMALGGEGWLNFMGNEFGHPEWIDFPRDGNGWSHHYCRRQWSLADTTHLRYKFLQAWDAAMMRLDEHYGFLSSPHQWVTHMDEREQILVFERGPLVFVFTWNPFGNYEGYRVAVPNPGKWRVALDSDAWDFGGMGRVGHDVDHFSDPEPAGTSRDRQHSIRVLAPSRTAIVYFNADTHTHWQQQQQQQQQQPEVEEEQQVQEQGLGHGNSLHGLAPAATQTAGGLSLGSGDEQSETLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM47</accession>
    <name>A0ABQ5SM47_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_015987</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051185">
        <property type="entry name" value="ASPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706:SF1">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="417"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="625"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="713"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="631"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="750"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI71070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="775" mass="81727" checksum="3A44BF8A5D436444" modified="2025-10-08" version="1">MVPEPSAVLRTSLWIPRPGKAVDLSTEEKRKQSVAAVWRGFFSRWEGPAAETIQKWWRGYTTRRFVQALRHGRLLKNGARERVEETAAVVIQRYWRGFLGRQKAAELRKAVVDIQRWWKGYRARKEWPRLRLRWRARNYDAITVQRWVRGHQARKLVRRMREERVKQQLQLQGDQGAASAAGRQTHGSMLSTAVAAGGAADSRREVAAIVIQAHWRGRQARKRRAWLQREDGKRKRYEKRLAASKVIQSYFRGWYVRRAIARLRVGGPLQPGSLASGVVAAPGIAGDVHATVTVSYGYYDDDDGTGGQSFEWPVPHGHPTPPPMPRAAAPPAPQMHTVRGGGGATVGGVQGYRGRATKKTEARLGTRTAVMSITAASANANATTTAVNVRPPLVPAGSALASIRAVRTAAAATAAAGTSNSASHQQQQSATQLSTIDDRNTRSTVQNPPPSPASRQGLLRMPQGTSGKLPPPPPLLAPSSSSDNSSFTAMSSPGPLRSAGASSGAIRTVVSQPPPSPVTPPRGLLQRSGPRTGGGHGRSGTVDRTVSCGPKPATAPAPLGRTLSLNNGGGGVGGVSSTVWNLGELRIRDPATDVSDAIADSPVPPVRIRPFGAATGGVTPAAFPTSHMTPRAGGASASRSASVASTSAKPTLPSRSDPLRASAPETLRSTTSAEGKDAKAAVAAVPSNTARRWAWDADGTLPVVAAAAATAAASAAAEPKTPGRRTSGSVEAATAAPDAARNPLHDQVMRANRGGRVVNGTVGGAAAAQRRKGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM75</accession>
    <name>A0ABQ5SM75_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="228"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="31268" checksum="9B48616BFA615330" modified="2025-10-08" version="1" fragment="single">MHRMKRLRQAKTGINGPDSNRSLQSLPPVLQQVIMSWAGRGGHGAFASCKALRAAFNTAIHSPDLLSRYCLSAWGPKNAVQRAFEVASTIFKYFSKQDYEQKLLGLLHGLAGKGATFHGYNIMDAICTGFVSVLGEVLQLLDSPSEEPERGMSKHVAACCSGLVNCIYLPQFNEEMVRLLVRAITVTAGVPIHFSTFAAAFREAAWHGRSSIIGALLEAAPPQRCINTALHYAAKTSHGDVVKQLFAAGATISEISTWAAFCGAHQAVVRPVVDAMRRSGAWGRDMAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMB5</accession>
    <name>A0ABQ5SMB5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000096">
        <property type="protein sequence ID" value="GLI70809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="106" mass="11360" checksum="6DF457316479F571" modified="2025-10-08" version="1" fragment="single">IFFTFSSLTLQVLNLDDVERLLGKRPFMCQELRNIDRFRHGSGASKAQELVTGEKTMETETGPGEEEGGAGGDGSGGAKENSDDTSGDEKRVEGKKGRRPGLVVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMF9</accession>
    <name>A0ABQ5SMF9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015281</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF13">
        <property type="entry name" value="AT30094P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="2">
        <location>
            <begin position="47"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="213" mass="23460" checksum="394F396D5DBE8A70" modified="2025-10-08" version="1">MAFTDFSCVYYNEGDTMGKILAYAALVPYVLILHHASQFYGRRDVYEGVIVAAFVLNESIARGLKHGLRHPRPIATCTKLDICDSFGMPSSHTQCIAFAFMLHLLLHLRGWGGKSLGTRLIGTCEVVGLAVAALLTAFSRVYLGYHSIDQVVIGGLLGGALGAVCFAILSWRGWDLIPSTRFGTLLHLKTSWVLSDPLRQEAAWYNDLRKKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMK2</accession>
    <name>A0ABQ5SMK2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FATC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FATC" evidence="1">
        <location>
            <begin position="111"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="143" mass="15177" checksum="FE2C3B5B51584E92" modified="2025-10-08" version="1" fragment="single">SGRSELSSGGGGGVVDEELEDGRVLAARLAGTRVDIALRKLARLHPSLIMVEELRCRHGVTPHFEALCGIVRGDGGGDAANAVAGSDGGRKDNSGGRTAYRARFGLLERSGPLTPEEQARCLIDLATDYAVLGRMYLGWRPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMM2</accession>
    <name>A0ABQ5SMM2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HPP transmembrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015758</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007065">
        <property type="entry name" value="HPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058581">
        <property type="entry name" value="TM_HPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33741">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN DDB_G0269096-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33741:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN DDB_G0269096-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04982">
        <property type="entry name" value="TM_HPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="457"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="512"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="536"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="577"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="HPP transmembrane region" evidence="3">
        <location>
            <begin position="451"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="616" mass="65358" checksum="784D81C3757A4838" modified="2025-10-08" version="1" fragment="single">LKCVTLNFEILSARTVQLAVDKTLSNSNTSSLYLKRGIECEKLLMNSAHTTVNGAADTPRGMKGPAPERCDAECADATRPGTDQEPEALLVFPTSVLPVDVNARSQRRTDLLRRALTSPWRRLAPSSAARPEGQAVVERSCHAFGASTIINHVGPAFLTDISAAQRMDRRSASSALSQRSLFTVDGLPEGPAPDPSIRTTNPSSHRTAVAIGGGGEGDFSISQHAHGPLHHAATALDTVVSRSADPIGTRRHLLRNQVREVQSEVRIQDPGCECETTDNVRNLMSYKSDPSLSPREQQPPLLQEQQNCTAQPYSGPQHGYTECGRRSTSPADNLQTSGGVGHYISRGGSGGIDRSSPANGNAAADLPTRGGDDVEVVASEGPFVVLEMKGPRGTPSTRAAAATAFLTGASASPAAPDAAVASAGMSLTLRGRMASYFSKWDGVGDTLMPADHPVDVFWSWLGAFLSILVVALLNQYLTPHVSLPLMIASFGASAVLLFGVPASKLAQPRNFLGGQVVSAIVGVLVRLAFMHAPHLVWLSASLGMSLALAAMQFTRTVHPPGGASALIASSALYIGPWYGFKFILTVFFGCLAMLVVALVVNNFSSRRRYPTYWMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMZ1</accession>
    <name>A0ABQ5SMZ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial</fullName>
            <ecNumber evidence="12">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_016562</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Mitochondrion inner membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="12"/>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050837">
        <property type="entry name" value="ComplexI_51kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011537">
        <property type="entry name" value="NADH-UbQ_OxRdtase_suF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054765">
        <property type="entry name" value="SLBB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01959">
        <property type="entry name" value="nuoF_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010120">
        <property type="entry name" value="PRK13596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22461">
        <property type="entry name" value="SLBB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="Nqo1 FMN-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142984">
        <property type="entry name" value="Nqo1 middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="Nqo1C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00644">
        <property type="entry name" value="COMPLEX1_51K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 12" id="KW-0288">FMN</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 12" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding" evidence="14">
        <location>
            <begin position="388"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI71372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="487" mass="52784" checksum="7C44A728DBDFEA35" modified="2025-10-08" version="1">MHRTAGIAAQFASVQLTGALQELKTGVLRAFSTQAPAAGTAATSPPPPPPAAKTSFGGLKDDDRIFQNIYGRHDTSIKGAMARGDWYMTKELVLKGRDWIIDQMKKSGLRGRGGAGFPSGLKWSFMPKASDGRPSYLVVNGDESEPGTCKDREIMRHEPHKLVEGCLIAGVAMGARAGYIYIRGEFVNERLSVMRAIDEAYAKGFLGKNACGSGVDFDLMVHYGAGAYICGEETALIESLEGKQGKPRLKPPFPAGVGLYGCPTTVTNVETVAVSPTILRRGPEWFSSFGRKNNAGTKLFCISGHVNRPCTVEEEMSIPLKELIERHAGGVRGGWDNLLAIIPGGSSVPMLPKKLCDAVLMDFDALKEAQSGLGTAAVIVMDKSTDVIDAIARLSYFYKHESCGQCTPCREGTGWLYDIMTRMKKGDARLEEIDMLWEITKQIEGHTICALGDAAAWPVQGLIRHFRHEMEDRIKNAGARQKIAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN64</accession>
    <name>A0ABQ5SN64_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cilium assembly protein DZIP1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016541</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032714">
        <property type="entry name" value="DZIP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051241">
        <property type="entry name" value="DZIP_RILPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21502:SF3">
        <property type="entry name" value="CILIUM ASSEMBLY PROTEIN DZIP1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21502">
        <property type="entry name" value="ZINC FINGER PROTEIN DZIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13815">
        <property type="entry name" value="Dzip-like_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cilium assembly protein DZIP1 N-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Cilium assembly protein DZIP1 N-terminal" evidence="2">
        <location>
            <begin position="170"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="302" mass="32880" checksum="563569F1353B6F75" modified="2025-10-08" version="1" fragment="single">MSTRQPIALSLSGFQFSYRNNYFDWRLLHGIDIDNVIRLTDVQAVEDCLDTLQRGAFAAERDRLSPANCVQLFRLLQLAIEYCAHLRAAHALLLDCYNVATATAESWKAAARTYLDVGSSFLSDSLGMGDVSREALDATDAAKSALEAADRDYNAALDALEAEDTRARHFRFRKQRTEISWSQLNAADADRLVASLDVGLLEAVLPNLTYGSITSEDDEQLTPHHFGQLIPLAQMPGRSRLRAVAGQRDRGTHGASHVGAAGGLCGHTHSHQCHAGAPWQHHLTAFGRASATRGAPSDGRCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNM9</accession>
    <name>A0ABQ5SNM9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_016878</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035910">
        <property type="entry name" value="RyR/IP3R_RIH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715:SF99">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13715">
        <property type="entry name" value="RYANODINE RECEPTOR AND IP3 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100909">
        <property type="entry name" value="IP3 receptor type 1 binding core, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2950"/>
            <end position="2969"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2976"/>
            <end position="2996"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="3016"/>
            <end position="3038"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="3098"/>
            <end position="3123"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,4,5-trisphosphate/ryanodine receptor" evidence="14">
        <location>
            <begin position="6"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="RIH" evidence="12">
        <location>
            <begin position="618"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="RIH" evidence="12">
        <location>
            <begin position="1372"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="domain" description="RyR/IP3R Homology associated" evidence="13">
        <location>
            <begin position="2414"/>
            <end position="2515"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="11">
        <location>
            <begin position="2957"/>
            <end position="3130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="996"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1856"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2322"/>
            <end position="2397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1005"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1865"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="2322"/>
            <end position="2333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="2384"/>
            <end position="2397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI71613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3236" mass="353252" checksum="8CF42374A7028E73" modified="2025-10-08" version="1">MEDLSTRCLKYGDVIYLKTDGKQAFVSAHDRTTPQVRTEELAEGADSPPDLKDCLFEVTKKYQYTQKKSLILELTRNGVVPEEALVSDSLSNYEEVLSATIAKPKVRLKLIALFNAYMREKRTNANEFRQVVGESVLYGQPVQLRHIPTGKFVTIKRTAADVERGALKVVLEEGGQEGSWFQVFSGYRTKPEGAKLMPGEVVVFKSLVFSGNGLHVSVGDIRDSSMLPVDKHPYVTSSKEVCASPDVTSYKIIPFSVNERVMPGLKDRLCGLSPVSIQDLVANTVYINFLGALNDKFLYFQSPLTRAEPNRISGPDCWRLELVMDGGFWTGMPLTHECVLRIRHLPTGTYLAAISNKAKEAARAWRIRKGSMAAAAAAGPANMATTVGAAGGDVNLSGGGGNAIPGGEFAGGAGAVAAAVAVGGYVPAGGAAAGTAVVGSLPYVAHWAGSADEDVLYDESFRLVLTKRYSVPDTLWEMHSFTKEEVVGTKVYSFFKHVATQFWLSGAGPPIPTQPSEDPDDPQEEQVYPVIHPEKLERNVMLVVKVPEDFTAKILDLQRVMSQLKDLRHVLHKEKIPPESELLDLVVNGQEAEAVAIIADTQYPDKFRQRYPALKRSLQDLVRWLDKKTWSEAADAVSPHIEYQSFVRELGLVDLLVQYMDAARVRLFATSSKLRGTRLGGWVLESGKLCHRILQLTCQGNEVNQAYLVKYAAMVMSHLSCPLTAPDTLAAMYEDNMAQMQAVSPAIVAASVNLIRSHGHRPRFINFLRQICGTRERPMPNNQNWIVADIMGSGRNPLQVFCQAGLETLQGADGQARRTWIIRCLRSDGTSSHIVVDAAQFTHDEENLTKRAIKEWDMSAEEGAYTTRELFIYYCFSLKFIVSLCYGRNSLARGQILAASAKYGLGLEYDALQAAVTDNFLPYAMRTYMVQVIRALYVDIEPYQPLKLPRHIRLLSKKGAIRVSLKTAANSETINALIRAVLEQLRLATTALAGDSGGSGGGGGDGKEDVGEQIGERGERTVGRNMLILNYLKTVHEMFKLGMLDLDSPVSQEMLVVLLDIIQKFDSLPLDRLERFERSYTSKVVMQAKKVALATIDFALDMKSEQQCAAVFDRFAQWQRSPERLEFWARLQAQVSRKGNATMKSGLRALNAGVRAVATLTNAATNKVLPLLPSAATSGNLESERGRNGSRGDMEAPAAVESFGDNEGVPHGGPEHWADESAAMLAADNEEGDFPLFKLDTLRDTAPLYTQGNSPVMKLFDLVRYDDQELTARTFQLLERLTSKREKLLEELMRTFVVTDDDLIALATWALKQVEIAQQAYNYMGSLDPAESQEACAKAAKAVDSLTSLLMPLQHITVSTGVPDAEATEKVFVSKTTAANCQYLMFDMQIHLMVLKFLKLPMKRKPQSDQRQIQQAEDPARREVFRACLEFLRHFMVVGDLEGTNVASHANQRVVLPSLELLLSFLDTHGLPASDTVIALFINNQADAATEGEKVIRKLIKLITRYGDKQHAGWLELLGKVMVVNGTPIKRNQMVAMQLVTQYGEDMLLLMSGDAGLEELRQLLASEHLTSRSTAVPPVSRLSYHTACTQLLADCCYGKEPELVVKASGYMSLGQVLDVLLLQPSAEIPEVNLRYVQRAYWRFVQHCYFATDTDNTKVQVRNGANRIWPLADVDAGGAGGDGEGGGELTAATSVLLQRFLDLPGGGEGSSKNRAGAAVDICLMHAVLAEVKRALADPSDALANADSATSFLVTSVFPALRDYFENHWQSHLAKLHSTPTGLLEDLFTNTMDLYDALKRLKDVANSQSVRAEIKLGLTNTRALLNAMPPEVNMTDRTFEPRPTGTLVLGGASRIGGGGGGGGGGSAPSTSQHTAMTASSQKLVQREWLQYLGALSDRVNVKIDLSTYEVVEVLRSGALVEAIKTAASSLGMGEGDTLFEQASLLRLARMLAACFGRLIPGTCPPISFEKLVQMLCELLGSRSSRPGAPRGVGPADWKPALLVKMVRAVCAAAALDDGAGAEDSAALRNAWASLGLLREGGEDGEVVTEWKQPSPELLRWRQCKYDKLGATRAAVTLLAHPTPEVQLEALKLLEVLLQSGNQAVQATIYDILKDGSELADAVFANVKAAFDRCRKYVARRVFVRNKEEARARKKDLAQEAKKAVTGAVKSLLTGGSRMAGAAGGLGLKAVRIVTSAPVELGSKVVMTSSTLAAAASKGLLGVINEPQPICSAGRQQGAGAFRAISDRNMGMVGAGGGSRKGVPRVSDGDYAVPYDGGAGPAAEAHERLDQPVAKVPGPTEAANVRQQQQQQRWRGIDTVAVAEGEEGDGDDEDGDGMLPGSVSVRQRNGWLGGGSSSGGRSSLRRQYDAVPVAEAVETTYSTGEPADGEGGDDDDDEEVRWEDKSAKLNNADNPYEFSSVLLRVLKSMVEGHHKNLQSVLQNQPQSTSSLDLVVEAVDLLNVLQDNLPISLVNGDNEVADLMVLVCLFVQEMVQGPCFNNQQSLAGTNFLASCNRIFGCIEYSQKYIPRPDDHSGEDRKNCFNKCNVKCALLNLLASLLEACPSQEVPGRCYDILDFDVIDRQIGQLCRVLGMAGEPPRYPQDGDDVADCDPDSPEDMQLIDISTVLENELLLFCAYVLKLNSVTPSRPPPLPYLSKLYNGEEVEKMDDLTPEQQLYAAELQSFLQRRLGYVEINWKGQLVEPCFFTLTPECSNLVTRQLWCDVTLDRINNTVSPDSRAFPTVKAAELVEVLEDVVDDIELESRLGRNRWLKAVSVVAQYRMRLLEATFYLAVGTLIFQALADARWNPKDSFSDRGSKWATRVLLEAVLVQSTCTVLLYTSFVYTELNKYMSHGVPKTILAIKAILERISEFFSAGGREEQGAAAASGGGGSAVSTGSKSLGGTLANVSAAAASAAATITSADGTLYDGASAARALGLPPPPLDQRRGWKDFILLAVALVVTISIYAKFWYFNMLVSASLAGFFVSPFFLVFHFTIYFLDFDSGKQLVMAIKRSGMNILNTFVLAVLAIYTFAVVTFLAFKDPTQVDKGDGPPCDTFYQCMGAHMLTGIMGDISALFNSDLWDTVPDKVNKDGRQQARTIFVLCFFMIWNFVLSNIFVGLIASAFEAIRDDQNTITSDRLSKCLICSQDMYLFNEKIQGGFEEHILKQHNALWYVFFLHHLRATAVEDYTGAESVVRAVLDNAKTTTDKGTWLPVSKSLAVMHAAELAAAREGGPATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNZ5</accession>
    <name>A0ABQ5SNZ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_016725</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF14">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047046344" description="Gamma-tubulin complex component" evidence="7">
        <location>
            <begin position="18"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="9">
        <location>
            <begin position="272"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="8">
        <location>
            <begin position="633"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="8">
        <location>
            <begin position="797"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="158"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="927"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1007"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="934"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1007"/>
            <end position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1099" mass="113479" checksum="A7D05F35B41F3130" modified="2025-10-08" version="1">MAAQVDVAGLLSRLVAALLEGSGVPENEIPQRQIPLLKMMAGILGSNAYPRSGLEGGGDDTALLHTLRKEMEAAGADKSKLDKLTELCNRLSPGGGRPGLVPRLRTSLLVLLRQLHREGRQMSGGGAAGQIAPTFSLSQAVLLGGLPATYAPPSTFLGLQQQQQGQQQQQQRNYPARPASVASYAGSTSGAGLLRPTSVVTPSEMGDEPTMLSTEGSRTSLMGTQGGGGGAENEFLTRSSSLAAMPGGGPSGDPLMTGVGVSMLATTEPSLVRDVLYAAQGVRGRVVGWVPGPPGGGVARDLVSGFRPDSATSLGLGPGRAMLLDRLTELGWLFRRVREHISSCTGPGQATVRQALGAALAAEVGDYYRLLALLEAQAALPLPTPGDTFDASNGGGLGASACSGNGQFLTLRRLVCWLSEPLRRMRLLAAIGDAAEGLEGGALAGAVYEFSRHGDPFVAANSAKLLQQVCVPLHGIIRRWVLEGVLDDPYNEFFVVQHAAAAVVSAALAAAGAAAGSLGVGTAVPPPLDLWRQTYGLDESRLPPFIGPSLAQRILRAGKAIHYLNLACGDGGWVQQRAEAWAREPPAATSATAAGGSGKVRGSKLAELEGLVSAAVRSVDERLMTVIWRHHRLRAHFAAIRRFLLLGQGDWVTAFMDLAQRELDKPASDVSEVQLNSCLRQALTATNVLGGGAGGALAAVASRGAVAAAGLVAAAGYGLTTVDTCPGGSGGEYDVDDDVAAMALLAERLRVRREKAAGAGETGWDIFSLYYNPAAGLPANVATGAAASTSIAATSGGPLSALFTNSAMQSYNRLARLLWRLKRAEHALSSTWGAAACGLQRMVDKMGADGYLAQPVLAMLLRLRSEMSHFATNLQYYIQFEVMEASWQEFYSRAAACTDLDSLIAAHEVHLTTLLRKAFLANDGPASGGGAGNAEGTPGAAAAAEGTVPGGAAGSPNVAALRRALQDAVANMVSLRAVASRLEELVAGGARLLAARASQAKARLARGGWGTTNGTTSGAPGGGGLPGVGTVEPPIPPAALTDLRQTLAELGAQHQRAVAEFTENLPEEAHDDVRFLLARLDFSAKGVQTSGNLGSAFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPK7</accession>
    <name>A0ABQ5SPK7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016840</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01856">
        <property type="entry name" value="YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019991">
        <property type="entry name" value="GTP-bd_ribosome_bgen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03596">
        <property type="entry name" value="GTPase_YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782:SF5">
        <property type="entry name" value="DAR GTPASE 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="4">
        <location>
            <begin position="238"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="475"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="475"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="526"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="589" mass="62197" checksum="98539D379B0B14F6" modified="2025-10-08" version="1">MKSITFSSSEVALRAACLTHTFAASTSPQCTAVVYPWRRPAQCSGVYPLGLFKSSLPSLNSRQRIAVGNGLFPSTAADDAPGELILDDQDAVAAAEIGGRNYLQLSDMSDESLQVRMVQWYPGHIARAERQLKAQLAMVDVVLEVRDCRIPVSSSHPQVAAWVGAKPCLLVLNRADEVSPADLAAWRQHHRSEGQRVFWTDGRRGEGVKGVRKELLKVASQINEKRAKRGLQPRAVRACVIGFPNIGKSALINRLVNRRAVASAPKPGVTRQLRWVRVDEQIDLLDAPGVIPASFPDQLAAQRLAICNDIGEAAYLASAVASALVLRVKQLAAAEVAAAAVVGIAEAEATSTVALGRRGSAGKKVALAATVTSTMTTSTTTGAAVSAGESECTGGGGVLARVLEAMQRRYGVELLTAGSAEDFVVAVAARLYGGDVERAGVRLLTDYRSGSLGRFALELPADLERRERREAGLRAAANRRERAREQHEQRSGSGASAATGDLPHLQTDPTGSADWEEAVPVGIPFSLSSSSSSRQSGVPRAVGRWEHGAGGAGGGGGSWSLHDAAINSSWVTEAEAWAKSAQGGPESTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPS9</accession>
    <name>A0ABQ5SPS9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="339"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="488"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="543"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="368"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="388"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="423"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="491"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="548"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="558"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="595" mass="58836" checksum="F3E0096F457D0594" modified="2025-10-08" version="1" fragment="single">GAFPLPWASVSTDLGLQYGQYGSGRLNTSPGFTRASSPAHPRPRSGAPGGPPQVLRLGPSRLGTPAGAAPGATYSGALAALLGTSGGSPTSRVSGSFASGALDELVAAAALAGGSTGGGGGAAALPVLELHSRAAELNDKVDDIEQNLRVERNKTAEMRRILADIKDRLNQRPLSGGGGGGDGSSLSPADSLTFPAANPLTFDSGRGPSHAGYSAFINTPSAAAAAEVSAGGGGGGLRYGSTAGVSISGPHYSDPTSGYQSKVRIIVDEDAIREQEQQRALQVLAQQAEKLKQQMLREHQERRLTASTAASAAAAGPSLQQMGPFAGASAPWTAHMIKDGSRHSRQASREANPALRRSGVRRSSPGKVSRPGSSSQVSSRQQPQHLHRVADSSDSDHNAREALAAAAAAAAHTDSRAGSRQGSAGGTTAGGGSRPPRPALSRLSGPTIHGSLGDLPAAAMYGISGSVATASLRPATVSGSTGYEAATSAGVGRGGGGGGGGGGGGSLHARAGDNFQDGRFAATAAFGSVSLVRSEKEAAAAAATAAAGSGGRGGGGNISKTSSFSDHTSPASQLSYRSASGWDAALPLAASAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQG4</accession>
    <name>A0ABQ5SQG4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_017086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000120">
        <property type="protein sequence ID" value="GLI71765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="19149" checksum="6184DDFFD086A1EA" modified="2025-10-08" version="1">MADAKAAKKEGGKKGVDLSGMSDLGGVKFFNVTLENTNGDLELVSIALEGMNAPVDEAAEERKGGAGHLGKMLLSAGDKTLAFLCNIPKELAESYPHVQATKWIDKLIEAAGGKVVTREESEEVIKVLVEGDPSNNLYPLKMRDAASNAGCAFLRENGLLPDDDSDDDYIPDPEAAGIEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQT3</accession>
    <name>A0ABQ5SQT3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VAN3-binding protein-like auxin canalisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_017042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="19462" checksum="99C07AB255F97BCB" modified="2025-10-08" version="1">MAARSDAAGAETCRPSGRTKRRGHPPRIVPSRGSRPLEGLADRCPMDRTASATSSGEEDEAGSNEPRPAGSELMYDTFRLMFWRAGTRSDSGAASLMVEVTIADAVSTALVAAAAGILAQLLVVTGDGAAAEAAAEIAVDAREAATEAPKCTAVVAAAGTPRVASEAAAMPAAVRTAAGSGTGLAAAAGPRKSGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM77</accession>
    <name>A0ABQ5RM77_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000446</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="23"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="433" mass="46045" checksum="40FDD5E8313A5CF4" modified="2025-10-08" version="1">MTIAFDNPQIRVNCSVNEVTESATQLSELPVELITAIFRLLDVDTVAKSASLVCRAWSVVARDAWMCRLSPRLQAQLAELAALGPSVSPIHLYLALSGRNFLRNPGFRRDANSVIVPPLGSLGAGRWKRWQRTAWVLNTSVGKEVSWEPAPLGMTADGSSLALDAPEPPLPYPGDDRLVSCMRRALKGLVKTLMHTQGGSFSQPGASAAAGDRGLEMPGLGPGPGTGQPLPPAATRQQQQQQMAALQGCDAAGPDAQQTSCGSSCLATLGTWSEVAQVIDLDWELQRRGLTAAQAAHLLDAGLSLTLSVHVGARSDTAAEYGVALMLDERDSRNGSGAAAADAIPSLQSFVQRPSRHLGYREREFVAPGTWERFEYVLDALPGGARRAIVMLRGRRSPTFSAGRNIGLGGRDAHSPSTRFCGAIFSSAQLVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RME3</accession>
    <name>A0ABQ5RME3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF444">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="439"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="524" mass="57176" checksum="2E7552A4A6BEFBEC" modified="2025-10-08" version="1" fragment="single">MKALLPCVLGHIERVLAGRITLDGRTGLLCFDPLCNLFTPRRPLSSIDLSNHVVLFIPSSLREMSEQLAVYRKCRRQYPGTAACVLLPAFCRRKAEASLRGMRHVHRFEPGTVLFNGVDREGMLAPWPGTSYSVDVRCEAPDEGAVGYGPALSALTLSAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVAEVNALGLTPESSDCPQVTLADGGKHPIVGRITLSLSLGPLRVVTRPYVLQGLAETAPYIMGSATLRQYRACMDMETATLHMCKGTLLIKVPFLSFEHVTPKEGEAEAVPMVNFAVTTMAARIEPELVGCKEAVRLLRKGAHGLLLRPRVSCCVTTAQISADPEVEALLSQYEDVFKDIPGLPPMHPVDHTIPLVPGAQPTARPMYRLSPLELDEVKRQVTDLLVKGMIRPSTSPYSAPILFVGKKDGTLRMCIDYRGLNANTVKNRYPLPRVDDLLDKLKGSAYFSSIDLQQGYNQIRIAASDIPKTAFRTPFGHFEYTVLSFGLTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMX9</accession>
    <name>A0ABQ5RMX9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="754" mass="77372" checksum="B71400FC262E93DC" modified="2025-10-08" version="1">MSAGNSYDAFISQLERKHQVEAQGHNSYFEVFNEASRLTDQLKRGLNVSIHRERLSEVRVAAQSWQESCYNERTEGNGVDILDWQPRYAHLDLSEGAGVAGSSGVGAGGSAVPGGPRASRSATAGFPIPPSVSHPRRRSNTSPSARRAGYSTAATVSGGEPPSPAAAVQLSAVDQAVSEPLTPACRSGYHHGGGLTPSPPGDPAGPARRNFPAGPGKVCRGRRATDSLMLHPRRGSSSLRLKDGRGRPSVASIKGDDGRQQRLSQQQQQNHQQLEQLEQQQQQQQPEEEDCGEESGEDVDQAGGVVGGERSFVGATGATTPRLPPLDEPSGPVLLSPTVMAHGGSSRADEAIRVFARTAYEPGEHRHTLLLMSAPEGFGGIGGDGPDSHYPERLPLAPIPRTVPRHVAEALSSLMLPSGIRASMTLNAEGTRVRASGGSFTTAMSPRAAALGGSGAGSGEGPPLLVLPGRAPRRASISCPWDPEPPNSLASPSRIRAAAAAAASRDSSGSNFAGPSGAHLSGNFRPARESYAGPSCGGGVISPTAASSCTKAPVLGGSSVSGETSASASVPGLIKGYAGGGPLDHRMLDMVPRLGLDAPRVKANMAAGPGALQMTGAVQSLAGEGEVQTTGVRFARGRRASATGIPGLAAPSGGVSLGPMMVTREQVDSYVAVHTQVPTGVYQGQVQCPGQSHSQLHGVGSAKGLVAAGSGRPGHNVIGTRGCLSDDAGPQAPRALRREMATSEAPGHVKGHET</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN00</accession>
    <name>A0ABQ5RN00_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000722</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019404">
        <property type="entry name" value="Mediator_Med11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22890">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="119"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="146"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="20100" checksum="4EA1225DCDEAE7C9" modified="2025-10-08" version="1">MSSISPPQPTEDYKRLQLIEQKVILCVQIAAQVTEHFSRVGRMSELAGPIQQLCTNFMNTIAEALQLAKEGAEASSWDHRLELHAYHSLARAHINTEKLQVLRLHLDTMQADLEAAVARPAETAEGGSEAVGTGAHAEAGPKQQPGNGGTGFVPGLGAGGESLRAVGSSGDGGGERGFQGGTSLGGSIMMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RND2</accession>
    <name>A0ABQ5RND2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_000900</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF7">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="499"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI59067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="568" mass="63708" checksum="C7B056149A4A1E0D" modified="2025-10-08" version="1">MEIYHQYIKLRKQFGRWPKLTDEGSEMLADIRPNEDHGREYIPRNPVTTVTQCVPEMSEHEANTNAVILVNKAMSHVEGGWPKDVDYTEAEHTIRYRKKVEKDEDYIKTVVQLGLSVEDLIKQNNAVDIYQEYFTNVTTDHTSEAPHVKTVTVFKDPNAVKRSASYVNWHPDGSIPKVVIAYSILQFQQQPAGMPLSSYIWDVNNPNTPEYELVPTSQICCAKFNLKDNNLVGAGQYNGQMAYFDVRKGTGPVEATPIDFSHRDPIYDFAWLQSKTGTECMTVSTDGMVYWWDIRKLAESVESMPLKEKNSENMVGGVVLEYDTNAGPTNFMVGTEQGQIFSCNRKAKNPVDRVKYVLSGHHGPIYGLRRNPFNSKYFLSIGDWTARVWVEDTAVKTPILTTRYHPTYLTGGTWSPSRPGVFFTIKMDGALDVWDLYYKHNEPTLTVQVSDLALTAFAVQESGGTVAVGTSDGCTSILHLSQGLSEAAPSEKSAISAMFEREQTREKNLEKAIKEAKVKARKEMARKDEVTDRVTEEQLRQLEDEFFKATGSSQALGAGASAADVGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP13</accession>
    <name>A0ABQ5RP13_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">indole-3-pyruvate monooxygenase</fullName>
            <ecNumber evidence="3">1.14.13.168</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001106</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>indole-3-pyruvate + NADPH + O2 + H(+) = (indol-3-yl)acetate + CO2 + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:30854"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.168"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.168" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF78">
        <property type="entry name" value="FLAVIN-CONTAINING MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="17"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="870"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="944"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1329"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1438"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1496"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1607"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1683"/>
            <end position="1719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2002"/>
            <end position="2021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2105"/>
            <end position="2125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2170"/>
            <end position="2193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2330"/>
            <end position="2413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2479"/>
            <end position="2505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2543"/>
            <end position="2562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2606"/>
            <end position="2700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2717"/>
            <end position="2738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2808"/>
            <end position="2849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2897"/>
            <end position="2927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="944"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1330"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1438"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1498"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1509"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1631"/>
            <end position="1643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1690"/>
            <end position="1708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2002"/>
            <end position="2014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2105"/>
            <end position="2119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="2173"/>
            <end position="2193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2330"/>
            <end position="2358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2371"/>
            <end position="2382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2383"/>
            <end position="2396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2608"/>
            <end position="2625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2717"/>
            <end position="2726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="2817"/>
            <end position="2835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="2837"/>
            <end position="2849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2927" mass="294227" checksum="983006BEF4C5CA67" modified="2025-10-08" version="1">MDGATRPSPPRSAISSVVVGAGVTGLVCARALLEAGVSVVVVEAAPDLFLGSASKRFSVDLINKYPDLAALINLPEFRPPTGVGPDDLHRYLLAYVEHFKLRQHIIFSCKLEHAQVGEDGRWCLVCSDLLDPQRLHRLTSDFLIVSAGACLSPTIPQLKGAAEFVGLHCHAQDLTELRDPASVVSGQHVVVAGDDRTAVECARRAVDGGAASVTLLYRQPHWPLPSKLLGQPLPHLVYSRCLPMMLFPPYYTSGGATRALSSMFAPLRRLFWWRLQRRLVRKYGVEEPTRPPLNVMRDMFYSSQASPDQDNWVGMLQGTSVRLVRGGIDRLGADCVILDDQTSLHATVLVHCSPYTRNYAVFDPELQSSLGIRKDGLHLYRGLVPPAVPSLAFLGCQTTSPYSWLTASLQAVWVAELASGRLQLPGLRDMRMDVLQQRRWRRRAFPPQHNRGSLVQWYAGPYHDQLLNDMGVAPVNVKTKGLSGCVPFFGSCMSYTPDDYRRLFCDAGEDASSLLYTSALSNKRRKTIDQASIGDDRETVELTLRGNGFSVTSNRSAATRTEAAGMSMTGPVTAISMSHSRRALLDRRTSMDRRQSIDTQRRLSNDAATRAALAQVSVTSNASRLQLLLNSRTSNLPAADTSEANAEPQFDFLTTNTACTPRVISPVTGLAVAVASSGGGGGGGGGLTATSRVSGAGALGQPPSMPSTRGSSIGCSRLGSQLNTNMGTAGAAAAAAAAPVVATGTLVADVALAYDASNSCRVNSDPEIEAVVAASQPSGDGAIEAAVAAHGYPGVVHGKPGLEAIVRQGAHPAPGEVASVLMGPRTLSPYQATAVGPAAASAAVNNEVSAQWRAVAAAAAAATTATIQCSGSHGAPLPQGRAVGDSTVTATASSPRALPGGSHQDLTPVAVLGQPWVGFTGLSSAIEVASFAVDGGASRHSLSAAMASGDDGGAAAATIRTTSGGSPEGSAGDIPAGVATTVSVLQGLTHGSFAESGSTLHWSSKPNAVRAAAGDSGSSGGGCSAQQQAMSDFASAGGAIRSSSSGGGVRRVHRDIGSLLPPSMLAGYEVLPIIDLYPHRQAAAAQTAAVQHLSLIPSATVRETTAQVAARTNADMNDHAAAAAAISSQSSRRFASGPFFSGCDGNGAVARAATTANLQSGQVAVTKVSGRGSGDGGGGGGGSLLVTSQSRNIPSSVLTAARSGIISRVSGGGFRAVAAAAAALTSGPQRAAQLRSPAAAAAANGHLASTSDVCLLDSPRLRPRAAQSPAPFGNNSLMIYLHERNSPPGGYGGGAAAARSGSNLTAAAAAPAGGGSGSIAVSGLHPQQLSSVGGAASSSTGKGVQRRRRRSSAAVFSSMLGDDDFMQLLLAAGNNDGRPEGVIQIQRQESCGEIDSREVAAATEGPVDGGALHATNVGSAMLPLMQRHPSVNSLLVTDRGSSSHGATSSGRTNSNIVHSPNVRTSRAGGVPRGVAAVAASLSAAGRSPVQLIAMGSSSSGGGGGGGTRGAATPAAGGAPRLTRMGSRNPGGFATSSGFISSGPMREMLDPALGSQNRGGVASGSWRRTGFVRRLTQQQVSLSGELPETPNRSTQSRNAVFDALLRPLFQQRQSSRVRETPSPHPSARRKTSSGSAVAAAATAAGGSGGDHDTRGGSGGGGWLLAVPKRDDECPPAAQRTRGCGCGIDGAEGPHDAAEDSRPGPREKQKAIGPSSSSSAAAATVPLLETVEADDDADMLATSSLLLGGSGSLAVSLGAALATLNASRLQEGGIGSICLMSRRTGDPATVVAVEAAEPEAPRPALSTICATGDEGMSTNLMLSMRPSAGGAGDTAAAAPGRSYVVRVSPVTTPPPPPTDTGAWTSLGTGLGNTPPQLLPSPSLHPTIASCGAMITHSDSAASATLLRVSPAAPGGQMKRSVSKQALVRGWNMLKSALGLRGSHALGGGGAEQPSTGRAAGGANIRLARCASGLDTPAHENRAAPVYDTSSSPRRMASISRFLRQSSHVSGTASGTGATPPHHSAASAGMAVAALQTYDIPMGTALCRSRSYCLPQSASQGGRGGGGRGIGGGGGEAGSGAAVNSAGNTIIGDMYGSGMAPEEYVRRGSGSFTSGSRPGRFSPQLQMGVSRVSSRDARSSMFLAAPGGPMVTSSSMRRKSALGFGALSRLNSTGTVPGGGGGGGGGGGGGGGGGGGSILSSIVRTGSISRGEGADTSATDYDYLRTVPGSPGLAAYEHDITASLACNVAAAAAATAPPPAAAAGDSILGSGGSVGADFTVNGASGKHFSCTSFETASAGRQAPRRLPLRQASRVVLAVDAAAAVLAAGIASPVSTTPRVPSHSASPPKNTSSGCIGVSSRSPALLAGPSVRSDAAATATPNTTAAHNSHPQQLPQNAQRPLSPYEVGVSGNDDASPAPSAFTSTWAALLCTPNVSSASSSAAASGMGTVPPSALLPAAVPVPPPGRLAGLVSSQSTRVLSAALQSPLSPPTQPSLHQDDPLHPSSSGAAGVALARQDLSPQGGGVFVGRAGSEGSYVIGGGGGAGGVTGLPSDPDNTFHPDNIDGVDTATATAAAAAVASQSYRVRAMPRRQQSLLRLLLNPIGSLGGNAGASSSTGYSSIGTSGSGNRDWMAAGGPGSASDRRPKRRPLKRVVSELSSSLRNTGAAGPSDARGDGSVVHLRLHPGGSSMGARPSATGLGFLHPQPASSASGYDFRDSYTSGGSASTFSPPHGTAGRVPRRVALHRRDSIIENLLAEMAGVGAGVGPGLRPPAEPSVGGQLPYSNSVAPAAATIAFDPHAPEGVISWERPRAWDGRDVGASGGGAAAGGGGRGTGAGSGEESRGRQGTRESVPRQFAAAAAVLPSVMLGHVAVPAAAAAAAAQTPLPLTAVSASALTLVPSSQPPPYGANPPDVSQVLGLPRRGTPRGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP54</accession>
    <name>A0ABQ5RP54_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_001157</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="335"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="253"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="508"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="600"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="627"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="744"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="814"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="852"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1100"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1163"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1407"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="566"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="635"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="647"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="678"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="778"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="830"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="874"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="885"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="909"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="947"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1114"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1431"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1455"/>
            <end position="1464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1484" mass="157785" checksum="E8A821B60A92AC8B" modified="2025-10-08" version="1">MVVPGRSYAWEGERMPHQRSSADGEPSSSGRLEFKRSGSSDLPLRSLAQLPRSSRNGSASLPMEPAEDVLLEKEEQTARRTTNLKARPPNTLERASTASTGRRARRGSIADFLGFKSGGPSQESSAAPSRDITLHGMAGPSASGARSRRSSFLNTFGIGTAQQIDLDNEAGDAGAVPLPEALMGSVDLTKSLQPPSQGRRRRASMLDIFRSMQSVETSVEGGEAGPPQGDSLARTKSSSKSRRASVIDFFRSSNTGLTTNGDDSAPSNASMNRRLQRGESAVGSRAQRRRSVIDILGHSSKKVLAKLMNHEFDQMDEDDVGVDGKLWFTSLNDFKPLTFKYLSGRYSSAFFACSVKTGQHYILKKFDKGKMNLADERGVRRALHFAEVLEHEHIVQCCGLWEDEQALYIVEEYAVKGDLLQDSMSHPEKYTEAFMATKVIKPLLDVLAYLHSANVVHRAIFPEYVMFGREDKFKLGHFTCAIDQRVDSPTERIPFLDYMAPEMLSVRSEDVNSPSMPSAPTVGKAKGRAARLPASSNDGSALQRSGPPPSGGTPVFDFGNVRDSRSSQQQQQQQQPGGPPRRPASTSGAQLLELFGLSGHTSEAADSGEKCSPISRTSTRSTFNLFRSSRRPSPQQQQQQQQQVQQMHSQVLSPSDDGNPSREASGCNGLGLVSGTRRLREESPGDAPRRMPTDGSVSRAVLYGNGGVGVSGAPARRRKSAVDEMSAPWLRPVGSDAQYDFSAEERNEDISSGYGKRRSSGGSDDGDTVAMQTPVVETTSAGATADGSGESSTAAAASGCNGCGSSSIVGDSSDAYGGSTSCSINPRPPSHMMKPSSPPMAVAASVAISAQLDSGSDHSPVEKLSPFPKDSLERPNSSPLSLVPQTQHQHQNQNQQQQQQRLAAGDSAASPSELTPPQRQVSSCRRSGAAHQPEVSTFPPGSPARTGGSGSSGCGSGTPLSPPLPRPCSALGSHLHRPGSAIGPAERADSRLDRAECLPGYAAERLTHHSSNGLRSQSTRLHSADSAQVPMASAVGAAMLAATGAIPPGPVMESLMAAEQTLTLPYDGRGAAHEPSDVKRVRATVSFTAASGAADAAASDSCAADSYVDDGRGTRSSRMASREPSTSSGQAGQVMMGLRTMARHITVRIGMVLGGRGDLLSPGASHGDSSAWGDAAESGGGGARDPSPRPSCPGAGEATAIIMPNNPWEWQEHYNEKVDLWQVGCLVHEILCLSLPFETEDKLLACALILWADIVSFPDHLSPECHDFMRACLTKNPAERPSAAELLQHPWIVRHAAGEVLKSVRQHKDDESLAALSNAQQLTVLQRAATAVGLGWMVGAGAPAAAAGTSVASSTGTNTNGSPSTPSWNRLSWLLRPWTSKVVPAADGGGVGEDVGDSKLALAMAMVQEQQQQQQHHHHHQHQHQQAGASGKDRSSRRKSSDVEAARRSLEIIRQHQHQQHLRHPGAVVGAGSPETPSRWEAMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP65</accession>
    <name>A0ABQ5RP65_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">von Hippel-Lindau disease tumour suppressor beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001194</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VHL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05468">
        <property type="entry name" value="pVHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.780">
        <property type="entry name" value="von Hippel-Lindau disease tumour suppressor, beta domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024053">
        <property type="entry name" value="VHL_beta_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037140">
        <property type="entry name" value="VHL_beta_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036208">
        <property type="entry name" value="VHL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022772">
        <property type="entry name" value="VHL_tumour_suppress_b/a_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01847">
        <property type="entry name" value="VHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49468">
        <property type="entry name" value="VHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="von Hippel-Lindau disease tumour suppressor beta" evidence="2">
        <location>
            <begin position="34"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="160" mass="17600" checksum="C513AA5447B07667" modified="2025-10-08" version="1" fragment="single">EPGVWLVKEEPQQHAVVEAAGGSNKMSETVLVFLRSLDSKKRTRVLFVNETGRSITAFWLNYAGQEVPYGTLLSGQAKVYETYVSHPWIFRCSTSGSRMLAGDRMAHYAEEMAVAPSGGLGAALGRRGTRQPQPQRVAIMAPPPLKHSRETHAAFPADYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPA6</accession>
    <name>A0ABQ5RPA6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="342" mass="34726" checksum="0B7ACF3866F4ACEB" modified="2025-10-08" version="1">MQRGGRPRSPDRWGGSPPGPEWDRDRSRSRSRSRSRSPRGAGGAGRFGMGRGGRGGGYGPGASYRGGGPQRYSGPGGGYSPGRDVHSDDSASSSSSGESGRGHRRATGRTRGPPGAVAGDGTAGGGAATRRETELLSRVGTLEHELSAQRVAHEADRKKLEFVSDKVRAVAERSKNRGNMLVRLVAALRRVDGAKAGVEAAQVELTRAQEDLNTMVQQATVMMEGEEAAFAAVKAELPTGIALQPVAPPPAATVAAVPAVVSTGGPPPPPPGDPPGGLAAGQAVGQAGGSLDRKVLEVTLEDQTAGGVATAAGGWSEAGVAGVSSDGRPKALSFKLRIPTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPB1</accession>
    <name>A0ABQ5RPB1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Geranylgeranyl diphosphate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_000977</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053378">
        <property type="entry name" value="Prenyl_diphosphate_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045485">
        <property type="entry name" value="FPPsyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43281">
        <property type="entry name" value="FARNESYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43281:SF1">
        <property type="entry name" value="FARNESYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="38648" checksum="903DBBC078BC3BE8" modified="2025-10-08" version="1">MQMQLQFPARQAGCSARRRDRVTRSCGLKPKQGQLVKASPIQRVVVRVATATAEQVELKPAGSFDFNLYMMDRAKMVNKALDEAVPQKYPDNLYESMRYSLLAGGKRVRPALCLAACELVGGDVTAALPAACAMEMVHTMSLIHDDLPSMDNDDFRRGRPTNHKVYGEDIAILSGDALLTFAFEHIARATKGVSADRVLRVIMELGRASGAEGLVAGQVVDIQSEDKSVGLDVLQFIHIHKTAALLEASVVCGAIAGGADEATIEKLRKYSRNIGLAFQVIDDILDVTQTTEQLGKTAAKDLAVNKTTYPKLLGLEKSKEVADQLIFEAIQQLDGFDAAKAAPLVALAKYIGYRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPZ0</accession>
    <name>A0ABQ5RPZ0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001485</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="390" mass="43870" checksum="D49982795E31BBDA" modified="2025-10-08" version="1">MDEGPRYPGSLVVQHVKQQTLELVDKYRRRRKRATELPRAASLLLAWEGAIVAGAFLVAIAHVAASAYMMERFDERTRRTSLVRYYQQRTELAAEAVRHVQESIVYYSRHAAAVERQLESTNTAARECRQRVLERRRQLEKELAAGLGREAGPSDSVGGSGTLASVLAPWRVFYVRLRLDGWRRELESLDREAGQLEQSLQHDQRLAEQARSKLRSLAKELRREAAAYEALHARLSAWRSYSCRSLVFSGGAQLGLPGLSWPGGSQQHGDRRAPGIEGIHKEDVEGGAARGDGDKDEDALNWGSSRTNSSEQMLWSWTMQVYTPAWMGDEESFDPPPPRGEAPGDRDDEEQEDEEGDNTGENELGKWEEGGIGEFLSEVRPLLENALSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ57</accession>
    <name>A0ABQ5RQ57_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="958"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="265"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="769"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1057" mass="114815" checksum="EABA4790A9C06F69" modified="2025-10-08" version="1">MDFRKTYAGHGLRSGSDPFAQLSKDVRQERDDHGRPEPNGGTRLNRPTQSSIAKGVGDSHAHRNSLSARASGRYTSLSQSVDLSPTVPSAGKSNIPRQASYQQQQGQHQYAPPSPPPPSATTPTRDVQLKLQDLVDAGQQRETLLLEQKRQLEASLHTANLRCTELARGLQKATAALDAERAVAALLRTERDEHAAELDRARSQAAELQVSGSVALTTSEVCRAALSSAFKGTVSAIGSAHEELESALAVQQQQHLELVAMRAHMEELEEVAYERGEELEAVREERRQLSDAMEELAERLQAREDELGRLVSLLVSARPEDAAGDSDGLYQAAVARAKGLTERVQQLEEQLGVHNSDLDALRSQVAGYQEQIARLQQDLMRINSSSFSSIKSISLEHQRTPADLEMQVTELANEREQLEVDNLQLAAENEQLRQDVSAMREQLEEALVTAAVFTAATDRDVSAAAELSRLQQQLAEAMDRANSFYAQAEAARVEAQAAERRAEQSAGLLLDFTSEAEAARAQANAARGALDAARVEGRKLRGELEVVRARETEARIEAEEARVKVERARAKVKAARLEVNTVKAEANDARRELEAVRAMVSLEVQQARAEGEERACMVERELLEALETRAAREQELAGEAAQLRNRVSELQDQLAADRRTSREALEQLNTVHANEGALMQRILAGEAERQQLLRQLDEGNGTLSLMRVRVDEAVQQAAEVTQLRTQVADLTAEAERQAGAAATMCARVAPLEAEVQELRATLRATAELAKQDARVNERQEAQLARLRQQVTEAEDQLRQSEEELRVLKAQVSVNERLCAHVDDLEARLLQAEAALTTTPSKTSLIDACTQVDTAVDNTVEVTNYGQGFVDSCSANGNPLSPIECLENPLRCCTLSASPRPTPHVQALAHAAAQQSLVLRWFDSSGIYGNAWGAGGDVRLFSTEHVVAENLLASYNAIPEEGPNDMDGATAKQYDKNSSSSTQRRHAGRVQPLRLMLGLAIKGTLVAGGMALGLALGNPRAFQALTCGSNGSEGGSGGRAANGDDDAVAVEEGMARLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQF5</accession>
    <name>A0ABQ5RQF5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044160">
        <property type="entry name" value="TGD4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34954">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34954:SF3">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="666"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="674"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="887" mass="89537" checksum="28FCADBDA80AB096" modified="2025-10-08" version="1">MTTGSSNEWLLGGFGNMPSSTLSPSTPLGIVPEKATLANQIRRTVPAQYWGLSVASASAPGGAMVSSVSNPAPLPLLTGSGLSRPHLTLATQAVKLPGRPSLPGLPSVCHDQGGLVLDRIIGHGSGESWWVALNGRARIGRLITQRADLAAAAADPASYSLCATSRVQVSRNTVLRARLDWCPPASTSSTGSASWLVRRRALRDDLLVKDSQAAGSGSSRSGWAAAPLSLSCQMQSRVGRWAEVRLDLAAGQRTASCAPFGIGPAAVGGGNSALTAATGGEPAVNEQALQSSSSAAAVGAVTTPLLLGLQAGSTTSSRSPLVWRCGVLQVTAPPTLPLITSHGPAVVTAKVADGAPSGPALQSAVYIQGTIALQGERVLWQGPKRPLLRRSRKEAGNGGAATPAASGPGSTTGAIAAVLSGGPLVAPPAAVPASTGPGTLIPLHSGSAGRSLPSASSGDEDEEVDDEDEEGSIAPYALAQGVVGGVGGMTGKALRPSRSQGLGLAAGAATATGSVSGGPGGGISSSGAAAAATLPFGIELPTAGTVQEALMDITHSVSRLRDDVTATTQWVGNGGLVEQLTAAAAAKRQAVSRPQQPDGLTTPIPWSSFVGEPHVKVAGIAGITARTPVILVNHGSAAPGGGGGSPSFRPMRLAWVQDLGLSHVLRRHGRSGDDDGDSGMPQDDDDTQGAGGTRSAEGLEAVRSALRPFASAAASVQAGRFSGWLLDFTRVAAQLDCGLWGPDAKSCEGNCAMGSGGRQRHPAFALGDIGAWHALSLSFSQQVIGPLRFVADWRYQLASSNPLALPPPTQVAPEAQIRSAASAAAAASSWFGSATGALASHTGGMRPQLLDAVYALDLAVPGLKGAARLVAWYSPQRREGMVELRMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQQ9</accession>
    <name>A0ABQ5RQQ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="14044" checksum="87A1D1A7449C012A" modified="2025-10-08" version="1">MPRLLVFPPPSPSLSRSVVVVPVLLSLPSAYGIRSPPSGSATTIQYNVNIKLYSVFISCICETSTSCDLVVMCGRGSSGGLSGGLAGREFSAQQLREFQLSDPATRRDLASRGSSGSSIRFGGGSTRGGGGRGGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQV9</accession>
    <name>A0ABQ5RQV9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Malate synthase</fullName>
            <ecNumber evidence="3 8">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_001539</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the malate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="3 8"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00727">
        <property type="entry name" value="malate_synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="entry name" value="Malate synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="entry name" value="Malate synthase, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019830">
        <property type="entry name" value="Malate_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048355">
        <property type="entry name" value="MS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048356">
        <property type="entry name" value="MS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01344">
        <property type="entry name" value="malate_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902:SF1">
        <property type="entry name" value="MALATE SYNTHASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20659">
        <property type="entry name" value="MS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20656">
        <property type="entry name" value="MS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="MS_TIM-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001363">
        <property type="entry name" value="Malate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="Malate synthase G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00510">
        <property type="entry name" value="MALATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Malate synthase N-terminal" evidence="10">
        <location>
            <begin position="8"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase TIM barrel" evidence="9">
        <location>
            <begin position="163"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Malate synthase C-terminal" evidence="11">
        <location>
            <begin position="419"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI59608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="546" mass="60663" checksum="CE91F1400383C06E" modified="2025-10-08" version="1">MAVQAVPGVAILGPVAAEHAVILTPEAQFFVATLQRVFNSRRKDLLKRRQERQKELDAGRLPDFLPETASVRASPAWRCAPPAPGLVDRRVEITGPVDRKMVINAFNSGATQYMADFEDSHAPTWSNNLDGHVNLRDAIRRTITYSAPNGKNYNLRNDGKLATLLVRPRGWHLEEAHFLVDGEPCSASLFDFGLFFFHNAKATLAVGEGPYFYLPKMESHLEARLWNDVFNAAQDMIRIPRGTIRATVLIETLLAAFEMEEILYELRDHSAGLNCGRWDYIFSFIKKLRNHRLYVLPDRSAVTMTSPFMDAYVRLLIKTCHKRGVHAMGGMAAQIPIKDDPAANAAALAKVRADKEREVIAGHDGTWVAHPDLVKIALEVFNKHMPQPNQLFVRRDDVNVTAADLLCVGGGQLLAEGGITERGLRDNLSVGLAYMESWLRGVGCVPIHNLMEDAATAEISRSQVWQWVRHGAITREGRVVSAPWVLQILQEEVEAAKGKMGPQRFAASKYPLAAQLLAGTITGGPYSDFLTTLCYDHIVTKPASRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQZ1</accession>
    <name>A0ABQ5RQZ1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001669</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050966">
        <property type="entry name" value="Glutamyl_endopeptidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15462">
        <property type="entry name" value="SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15462:SF8">
        <property type="entry name" value="SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="172" mass="17967" checksum="F19376E80DDE74C6" modified="2025-10-08" version="1" fragment="single">GEPVIVSLGAQYADGISLNTTRGAGNPDGAMGGEEPCLNFAGYPTPLVRWAQYCRYLANLDNNNFVETTCKGRPGVSGAPLWVYVPMSANTTTTGSSNTTTTGGRKNTRTGSSRSSGGGGGTSQAVQQDRRVMRAVLTAGVPGGPTGAVRIDSVLRRWVRQVQQKMPCDGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRV6</accession>
    <name>A0ABQ5RRV6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002257</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF963">
        <property type="entry name" value="AT08303P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="597"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="846" mass="87577" checksum="4E93AF52B53F3A55" modified="2025-10-08" version="1">MEERNRGDHRGAHPHASYGCGRIGACFGHLAGSSCRPRLPYCCTKGSFGNAGDTKPSPGGATPPTRQHVDADQMEASPPLMPCHGPPLRWLATAPSAAAVSPAAAAPATVRVALSRVASEKGPRFSDIDSDGLRDVLLLQVPYTRADGEGGGDGAPAGDITAVLLRDYPSVYDASSMTPYQRSMPSPASSPMELRQLSFLAPPSLRGSISVCGMFEQDSSHAASILGTGPFRTFPLAGGGDSGGGGGGGGGGGGRAAASGGPGVTATGTTGNVPSARAVLLARSRISSTNSYTSTTRTSGSRPSRWGIRQLMGLVSAGFASLRQHLGRLVEPASGSMRPEAVAELTRWTSRRDLVRAIAQVTDLQVVSQNASRSVVCCGKAGGSQVFIKYTAGPGRGEGMAAPATEGLLSRVLVHPNVVRTYGWHLTQVAAEDMSGDLEAALAAAGAADGRKRRQREEQRQPSPGPPERQLSQPNQQSKLHLPPQRSLMALTKGPPKHSPKERSLLLRSLLRKPLSCGETAEGGDGNTVGCSGRQQLWSARCRTPEAAGESLLHGTRTVAITPDGVLYGKPAGEDGGAAAAIGHGSYNGYKSAPASILLTRTVTAGSCSTAINPMYNVQGGSVSGGEAANRRQLSVGTFPPDLGSAAVRTLVPNNGRTTLQAGSRITKSDYALCELTGVATAVNCATVAVVMGEVNNDGAASTAAATSPCPIPPLTRTQAVNAWREMQDMLQRLNARPGDYIARIVMEAADLGSLLTAIRQGIFSGNNASTSRLHALLLTARDVSRGMAHLHRHTILHGDLKPSNVLLRSDPTDPRGFVALCRSACTATSAPVGTWDNPGISNVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS92</accession>
    <name>A0ABQ5RS92_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02954">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052:SF5">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017199">
        <property type="entry name" value="mRNA_splic_U5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01410">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="142" mass="16587" checksum="CAF5679CA91E4B3B" modified="2026-01-28" version="1">MSYLLPHLHSGYAVDQAILAEEDRVVIIRFGHDWEPNCMQMDEVLASVSDQMKNFAVIYLVDITEVPDFNTMYELYDPCTIMFFFRNKHIMIDLGTGNNNKINWAMTDKQEFIDIVEVVYRGARKGRGLVVSPKDYSTKYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSX2</accession>
    <name>A0ABQ5RSX2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aconitate hydratase, mitochondrial</fullName>
            <shortName evidence="12">Aconitase</shortName>
            <ecNumber evidence="12">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_002708</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="12"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01578">
        <property type="entry name" value="AcnA_Mitochon_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01584">
        <property type="entry name" value="AcnA_Mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="entry name" value="Aconitase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006248">
        <property type="entry name" value="Aconitase_mito-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01340">
        <property type="entry name" value="aconitase_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005558">
        <property type="entry name" value="PRK07229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF3">
        <property type="entry name" value="ACONITATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0408">Iron</keyword>
    <keyword evidence="8 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="13">
        <location>
            <begin position="83"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="14">
        <location>
            <begin position="613"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI60551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="813" mass="87419" checksum="633F67202B6BEEE4" modified="2025-10-08" version="1" fragment="single">RISRQQITMQSQPLARIGLGPVRLGLLGRERQNVLCYSSISGIEKVPMSKFEPSEYVNDRYKAIEDRLKVVRKRLNQPLTLAEKIVYGHLDDPETAEMKRGVSYLKLRPDRVAMQDATAQMAMLQFISSGLPKTMVPSTIHCDHLIEGTTSPQPDRGGSFGGEADLKHAVTTNKEVYDFLSTAGSKYGVGFWRPGSGIIHQIVLENYAIPGLMMIGTDSHTPNAGGLGVCAVGVGGADAVDVMAGLPWELKAPKVIGVKLTGKMSRWTSPKDVILKVAGILTVKGGTGAIVEYFGPGVDHMSCTGMATVCNMGAEIGATTSVFPYNKRMHDYLVATGRASAATLADAFKEHLRADEGATYDQLIEINLSELEPHINGPFTPDLAHPLSKFAEELRKNNWPTELKAGLIGSCTNSSYEDMARAASVARQALSAGIKAKVPFTISPGSEQIRATIARDGIMDVFDKIGGTVLSNSCGPCIGQWKRTDVPKGEANSIITSFNRNFAARNDGNPATHAFVASPELVTAYAIAGDLTFNPEKDTLVGADGKEIKLEAPFGDELPAHGFDAGANVYQAPPESLGERSKLSVKVDPASQRLQLLSPFKPWNGDDIKDSVVLIKVKGKCTTDHISMAGPWLKYRGHLDNISNNLLIGAVNIANGKPNAVKNVVTGEEGPVPATARDYKARGLSWVVVGDENYGEGSSREHAALEPRHLGGVAIIVKSFARIHETNLKKQGMLPLTFANPADYDKIDPTDRISIEGLKTFTPGVPLTLVGKKADGTSYSFPVNHTFNENQINWFKNGSALNAMAAAFKAGKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT32</accession>
    <name>A0ABQ5RT32_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002999</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="2">
        <location>
            <begin position="197"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="398" mass="44417" checksum="80C3D7303A364F2A" modified="2025-10-08" version="1">MTIVAHSLTLPVDPPAYYRRDWCGWCRCPCCCSCCGWPRRKCGCGRRGWYRWCSLIPAAGAPAGDAGVAGMDDGEVPPLVEDDDEGEEEEEEEDNGESESEGCDDNDDAAGGDGASGSGSESGQGCSGDTGDSGIPDPTPPRPVRQRKPPDWQKDYVMLAIGPEIEEPRSLKEAQSSVDWPQWKAAMDEEMASLHENRTWELEEVPMGARKIGLKWVFKIKRDADGNVERYKARLVAKGFTQKEGVDFGEVFTPVWKYASFRALLAVTADRDLELHQLDIKTAFLHGELVEEVYTEQPPSYQLGGPNVVCKLRWALYGLHQAPRAWYVRLRKELESMGFKPSLADPGLFVKTENGEPVYILVYMDNLLVVCKSLAVMGRWWCRYPPMYRQVRGAPHGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTZ7</accession>
    <name>A0ABQ5RTZ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TRP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003022</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046422553" description="TRP C-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="20776" checksum="F0D37F29ACB100FA" modified="2025-10-08" version="1">MARLHIHPVGLFLLFFGLLSWVVALGGLGASTKDCTERAKSKAASCANTYQLEWWSIWFEFILLVIMILTCFTVAFDRARHIFLTYLGMVTVLLTIMARNFITNSFTLINQADGKLFGTYRTDAYSAAAAGAVLLCITNFALIIFVGLGAAAPANPYASLSNTPAPASLQTGSTAPVEQKYQPSTYQPSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUJ0</accession>
    <name>A0ABQ5RUJ0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="193" mass="21651" checksum="172D57F5F0209CDF" modified="2026-01-28" version="1">MATPDVKLFGKWSFEDIEVQDISLEDYIAVKTKYAVYVPHTAGRYQKKRFRKALCPIVERLCNSLMMHGRNNGKKLMAVRIVKHAFDIIHLVTDQNPIQVLVDAIINSGPREDATRIGSAGVVRRQAVDISPLRRVNQAIYLLTTGAREAAFRNIKTIAECLADELVNAAKGSSNSYAIKKKDEIERVAKANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW77</accession>
    <name>A0ABQ5RW77_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Spindle and centriole-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="565"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="626"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="783"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="804"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="838"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="908" mass="94852" checksum="65EDD2664085CFE2" modified="2025-10-08" version="1">MKKSSYHVNPAVNKWEEEQRRARMEKMIQRAKSTLPPEARGGSKPSGPKKPSPYSAENNATKVIQKRSTGGQGTPAGAAAMDVQELDSLIYNRVQEILGTPLMRRAAAARAASKGAAILNTPQSGRGGRRPAWNEDWNAQTGPSPPAKHEERCISDAGARRGTSNPPYERSSPKSTSGSTRAGPSHVPSGSTPTKGKRIPAPSHINIPVSGIGTGVPLQRRPGTRMQAAEERVVLLPSPSPAATATKSPIVRPSSSRYGSAAGALAGGEAVEPPAGTSDQLLGKSMVAAIEALNPQALAARVAVSRITQNQRSHGCERLGYVPDASSTGPGSIGGVSAAASGSYHDRMGRKPLSDSVSGGGAAGVSSRDASLMQSLAAAAGVSQGLLGATSVTSSSRRPPLAPAQQRASGRGIHQAPQGGGLAFKGRGGRDDAEEDNDDEDNAYTDDDFEEYDSTEDEHQHLAQQLEAEPGNEEGLVMPREYDGGAGSGAPDIALLRDSVNALNELVQLKIQQELARGQQQQQQQQQQVDPSLNRQSLQVGKVIDPGEQRQVSPPDMLASLTLVGSEESDGRDGAHGPGRIDPTMSSLGDGLRQSDLDYLARLGGGEPHDGLEVATSTDERPFIIEPGAPAGTAKAWGDRHAWTTEKGADSGGTGLQKLRDRQDRERQHQGFSAGGQRRHNQQGLHGGETQLSRKEAEPTIRVPGASGAVADQGDEDSVLLGETLTPRHRTGTAAQMQAVSNALAALASSGQLSSGDAHELRQQTDALYGSLVQLHDLMNGPRGAELCSSSPTRGDSGAAGFTPTAQSPEDSPSQSLRRQAAGAPKGSVESYPARPTLQRTNNLGDGETSTWGPKPADVTEGAARNAAPQLTPKHRLIADDVAWMAGLMQSLSKSLQVDNGEVAMLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWV6</accession>
    <name>A0ABQ5RWV6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="130" mass="13944" checksum="456B691980E28BB6" modified="2025-10-08" version="1" fragment="single">RIAMEDLNVTDRQQELINALVQRKQIESSKTSFTVPATPVPVGAGGTAQHTPGLGALELPKGTTKSGRNDEPPGTQDGRTSQRQKQPAHGEDALMMVLAANAARIHQKEKEIKAARRRQVSPNNSGKESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX25</accession>
    <name>A0ABQ5RX25_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004784</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF33">
        <property type="entry name" value="HELP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1093"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="5">
        <location>
            <begin position="1104"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1524"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1535"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1547" mass="170548" checksum="3B936F761353C0E0" modified="2025-10-08" version="1" fragment="single">MAESLGGSRGRNDNTGTFKSFGFTKTQGQMGRGGGILDRPATARAGTPSRGGGVAAVLGGGGGSYGGVGRPRRPSSAPRERPAGDKSMAARARVEEVRQVREAQYWGREDQEPTLRANKPDVPPFWSIREHGQPFNDERLAAGVTNFFRLAQPAGLTTPQDPMITALGPATMQQAAMRKSLESGKRHFTSISDLAPLPLGAAATETLSDAEREFLLNRLTPYQRIHALHQCLDKDQSSACKTWVPWPLAILNRETLVAQQEGRKARPPKYEMSDWNGGPAQRDMLMRLAIAELEYMMRRHLKFMSDWACNAARAPSALEKDILRQAFWRVDPQRTGAVSIQQFLQVWQNILRLMEYDDVWSKGKDVKGKVKHKQVLKPLRTVLLDRNMAAALFVKYGFDKDGLLPYVVYINALCETPSRLLGHEIVLKKKERGKNGLEDDLDIAMCLGNAKIEYRYCKAGVFPPGEFTAQMGRRSFKPPKGHLWLEHVYGYAGMLDYTRQSNIFYTHNTGKPLNPNTQKAVIRSLLAAQGSAEGTPMRAEQLKRAEFVYYTGSVGVVFDKEKYDAGLPCQRFFFGHNNDIQCLTIHPNRRWVATGQQKQTGPTEVPYVCIWDVDHCMQLQRLDHDRDERGVIALCFSGDLLEGKGGDLLLTVTSDDKHSIHVWRWLPHSNKYINAHYIPGWSLGPEKKIRPLRTATKNTPGEGGSGVGEMYCSSSYSIMEGEVLNDAWLGNYPFDLDNWKAACVGGNQGQTGHGSEYDAVQAILGASRQPLHLDRPAWEQEAEWWTQNPLATPTPGSEVGPCGMWSHLEHPDMRVRADDADGMYMSMRNVKREEGFLRPGTGEDKLKMPHLEQSFPGCNGTPPMVYGVVWCGLKPSSGRRGSEFASYGIKHLKVWIANDEGRFIGTPGSFGTAHIENVLSALYVPATHAMRSPGDSCLLTGFASGKLGLWVPPYPTRAGAVYTLVRQFNAHSPGKLITLVDGTQVNAGVRALGLRKRAEGGLEVLSGGADGCVRVWPLEDLSGARPDGSPMKGLKLGKQSAEFKLVTPSTGLRKEEDPIVVAVDCHASLDREFLAGTDGCDIWEVDADPRIIVEGHEDPLDEVTAHPESPYTFATACDSGKLRVWDARRREVAKSANLGYALCGIAFSQEHLPFLDEGKRGALYDGYHLAVSGRAGELTVMRSDTLQPLVHRQDVSNRQAIPEMKYSPLGGPRLLAAACSDANVYIYRADKNYQLLCKCVGHSSTVTNVDWSLPVLLHGTHMDGKFVLQSCDASGSLLYWDPTTGKKLPYNMRDTEWYTWTLPVGFDVMGIWPDCSDGTDVNAVDRARIGTPIYDPYEAAYENSTEAAMHYSERDSADGICGAGFVVTADDFATVKLFNFPVVADDAPYKAFRGHASHVTSVRFLADDRLVVSVGGSDRGIYQWRTCGVASGLPPLPAIFRMLADCETKGGQQTEASKVLREELWDRLHTRSRAFRSYERLKLFAMRVVVEEKRVNSLASAKPQAKLPEQLVWAKVPGSTNQYGPIPRSMLEAAEGGGKASKGTSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXJ3</accession>
    <name>A0ABQ5RXJ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Threonine dehydratase</fullName>
            <ecNumber evidence="11">4.3.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Threonine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_004956</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="11"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1020.10">
        <property type="entry name" value="Biosynthetic Threonine Deaminase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001721">
        <property type="entry name" value="TD_ACT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038110">
        <property type="entry name" value="TD_ACT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005787">
        <property type="entry name" value="Thr_deHydtase_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01124">
        <property type="entry name" value="ilvA_2Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006674">
        <property type="entry name" value="PRK09224.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF11">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00585">
        <property type="entry name" value="Thr_dehydrat_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51672">
        <property type="entry name" value="ACT_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT-like" evidence="13">
        <location>
            <begin position="459"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="ACT-like" evidence="13">
        <location>
            <begin position="561"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI62342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="649" mass="69505" checksum="1927125F1FF5821B" modified="2025-10-08" version="1">MDMSLHPSTSQGSFCTSRPQPFVVHRPSWKGLSSPQPRTAEIICRTIAGERPQVAPSPAGSNGTQPNVAPSSSQTSICVPEDFVLSPGELSPVNRTGLNLPEDVFRCFGCTQAACQGPEGCAANKWRFETDGYLREILTARVYDVAIQSPLEEARKLSEAAGNKILLKREDLQPVFSFKLRGAYNKMAKLPAEARARGVITSSAGNHAQGVALAASRMGCAAIICMPVNTPEIKVANVRKLGGQVVLVGESYQEAQAAAVARAAAEGLTFIPPYDDPYTIAGQGTIGDEIMRQVSDPDKLDAIFVPVGGGGLIAGIAAFTKALKPGIKVFGVEPTGANAMAISLARGERVALSRVDAFADGVAVKQVGQETFRLCRELVDGVVLVDNSAVSAAIKDVFNETRSILEPAGALAVAGAKAWLKRHGMKDATVVAVTSGANMNFERLRLVAELANVGGRTEATITTTIPERPGAFKEFITTALSSDSDLSVTEFKYRYSAGSTAQVLWGAGIRCHDQLTVLLDRLNAAGMTSTDISGLEVAQVHLRHLAGGRARSYTGRIENEKILQVVFPERPGALRRFLDVVSPAWNVTLFHYRNSGNRESSVLLGVQVPPYEEERFQSALHSLRGHGEESFAFKELQGAVREVFDKFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXJ4</accession>
    <name>A0ABQ5RXJ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="33077" checksum="AF00096D74ECB872" modified="2025-10-08" version="1" fragment="single">MASSSVEPRGPLDTFAEDLGRAVLDAAASGNLSAPALLAAATETGVRPDQLATLIAQTTEIRTQLGNYLLSLLNLLKSTVAVNTNMDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTDKTPGHSIADATRAYVRDAKKYLQAVHGGDNDGIRCLFVGNTLEGAAKDWYRNWTNARGSYTFDELVAALLARFATEVQARDVEARNVLAAGSYHMRRDENVPAYQSRFEALVTPIADLTESERVFWFRRGLTKLLAKWCATDLVGAQIKSYDALVAFAKGAEIRLLAGEEEVKSAPRANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY45</accession>
    <name>A0ABQ5RY45_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase delta/zeta catalytic subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_005199</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030559">
        <property type="entry name" value="PolZ_Rev3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812:SF1">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="3">
        <location>
            <begin position="47"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="213" mass="22770" checksum="2FE9AA70EFDD67D8" modified="2025-10-08" version="1" fragment="single">LRRMALAIETAVRASAIPGQYQQQQAGHQQNDGGGGGVGPTTTATPKQRQVVHALQLVRGRPFYGYHIDEQLYIKVVLYNPQDVSRVANMLAGGAIMGLRLQPHESHLPFLLQFKIDFNLLGMGLLKLDRVIFRGLLPTRARPPAPGWEFLRDTYAGEEDAEAEEADDITGGQQDGVRYVPGAAAGTWRDATPHGNNASGSATSDSGAAAAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY64</accession>
    <name>A0ABQ5RY64_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Solute carrier family 40 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004575</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43266">
        <property type="entry name" value="MACROLIDE-EFFLUX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43266:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="249"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="56392" checksum="68CB729F5AD7A381" modified="2025-10-08" version="1">MTAQATRIQDYALCLWRNNIYAFMWLAECTKQAGNWFTYVAAYDIIAELSGDDAALHTALFLVCRRLPYLLLFPLSGLAADRLNRGSLLIAACLLQSAVSFSLPLVRMSRQIELLYPLVLLQYSAQAFYDPARIASVPTVVPPAMLCVASTLDLMGFSLMAIFGAAMAGRTAAAYGTAVCFRTEGCTFLVAASLLLRLAAWRAPNPHEAASSPRQHSVSRGRYRESYDTNNADGAAKVTEGEAVATAVAATAAASRERGDNDQSEKPPELQPLLVPPPPPPSAVQEPGAEQPRRSCGCGGFGALHFMSERRNWDVAALVWCKATGALAWGAADVLNGRFSLMSSMQTLGGPEVTLGAILAAVGLGSVAGPMVANALTPGVARWWRISIAVAFGFLAAGYAMMASAVTIGSVLPATAIRTAGSATLYIHSTATLQHRLPDSVRGRLFAIEFVLWTVSEASSTLFGGYALDSLGWSTRHLSMALSLMAALFAVCWSINAWVLWGRDSREGPLREALLEADRTDIEVADDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY73</accession>
    <name>A0ABQ5RY73_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="110" mass="11190" checksum="7E6D2130DE93D56D" modified="2025-10-08" version="1" fragment="single">MREVRQGCETPTLPGRNSSNGSSSPAIGCPRAMRPSWVRFLDRTTAISSRNINSRSTVGTMAASACASAPSAAAITVVGVVCEDVATTNSSRAKVGSPIPASLNTHDAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYH7</accession>
    <name>A0ABQ5RYH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052648">
        <property type="entry name" value="Ser-tRNA(Sec)_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20873">
        <property type="entry name" value="L-SERYL-TRNA(SEC) KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20873:SF0">
        <property type="entry name" value="L-SERYL-TRNA(SEC) KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="534" mass="57048" checksum="BEEBBC8AB1A74D57" modified="2025-10-08" version="1">MWTTRSIKNVASKYSLASLSMLSHQQNIKMNYPKVIRAKCALLVLCGLPGSGKTTLVAKVRELVAQNNGGLVPSGPPCIVEVITFDDWLHSALLTTGGFSADAWQRSRQAALNQLRNLLTKSSTSDEGDSQYTLLIADDNHHLRSMRYELFRTARDHGAAFLQLYFPCCPWEALKRNQRRSGIAVVPAEAVLRMAEVLEAPQPERFSWEANSLAIQIAGCEGTGESGGDGDTPDRGCRRSIVAGGDINSGIDMGNAGDDNNKSGENAAEATCLKRDNGWQCHGAVSGETDCTKPGPSPHPGSQRAHSPESSPLLPQQTQLQPQLHIQSHQVSKDQEKAGLQNAHPGVQVQAGDITALCAALWRLWGPTPPTPPREEDLLRRREEGRAANTASILHSLDLRSRRVLGAAVAAAAPAERAAVARALNGLRRVLLQTAREVLQLLPLQNLVYESEGCCRCGADGDDCAGGDGGGGRWEAVAVAVAEAEGEGHVLRRLQELEGEFVRAAGQGDDSRAPTVSGEHSPGSVSDTSSKGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYK2</accession>
    <name>A0ABQ5RYK2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">formate--tetrahydrofolate ligase</fullName>
            <ecNumber evidence="2">6.3.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_005139</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.3" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="637" mass="67849" checksum="9364759F25E7E700" modified="2025-10-08" version="1">MAATEYKPLLPVKQPVPSDIEIAQSVEPAHIAKIAEAVGLLPHEYDLYGTHKAKVKLSVRDRVAGNPDGKYVVVAGITPTPLGEGKSTTTIGLCQALGAYLKKKVLTCVRQPSQGPTFGIKGGAAGGGYSQVIPMEEMNLHLTGDIHAITAANNLLAAAIDVRMFHEANQTDEALFNRLCPADKQGKRKFAAVMLRRLAKLGIDKTDPDELTPEERRDFSRLDIDPVSITWRRVIDTNDRFLRSITVGQGPEEKGATRSSGFDITVSSEIMAVLALATDLGDMRERLGRMVVGNDTKGRPVTADDLGVGGALTVLMKDAIMPTLMQTLEQTPVLVHAGPFANIAHGNSSVVADQIGLKLVGEDGFVVTEAGFGADIGMEKFMNIKCRASGLTPDCVVLVATVRALKMHGGGPPVVAGTPLHHDYTTENVPLVTDGCCNLARHIENARKYGVPVVVAINRFASDTDAELQAIKEAALAAGASDAVICAHHGLGGAGAVGLGEAVIKACSGPKPEFKFLYDVNISIKEKIETIAKEMYHAAGVEYKPEAEAQIEKYTRMGFDKLPICMAKTQYSFSHDASLKGAPSGFILPIRDVRASVGAGFLYPLVGNMMTMPGLPTRPCFYDIDLDTATGKVLGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYT0</accession>
    <name>A0ABQ5RYT0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="817"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="376"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="545"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="842"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1046" mass="103933" checksum="CA318883F93D876D" modified="2025-10-08" version="1">MAEVVRPGMAAAPMRHPPSVFAEVQQQPDGADLNLESLLPSDLTFAIEFAQPVKPSKPPAYKPTALPTQLLQPSTSAVIPPNNPWSYDPAETTGRSNSCAQYQQQSTSLLVTSRSMPDRSAYTQHHQNSHHQLNNYQHNLHHGTATTPAAALSPRPPVTLPPPPPPPPPLPRGCTTASAATPPSLPVSPSGPVGISTSSSSSASPRQSPVHSRSGNSDYGSGASPEDYYAIGQPPPPPPREVAAAAVRTGDGSTGNRGPVSSRSFVRRNSAGSGGNYNCAPPLPPPPPPPPRDGGNTGNITFCPPLDLGALTTSLCDGSVLVTQNSRGGAAGTTAVAPGPESWPPPPPPPPSKNNTPRPPPPPPPPRSDPERLRSRSSSSSAGPYVSGYPAVVPPPPPPPPRHSAGVGQATEAMLGIDVTIAGDADIKSSFKVPTVQEAQMLVAAALQDGTDSSLAAALELSSKLQSLAAQRIAAAALPGNSSRQPSSTPNPTALASAPGGARSPTALGGTNGRGSYCGQCTQPPPPPPPQLAPADSGRLASMGSGASNLYSRTSTTPRSGNCYVPPPPPPPPPPRHFNSGSGVVTQATGGQTSSPPSVSLPPPPPPPRAPVPQPPSADVAAVMAALPEVRSPFALYQRASDDDDFCPTPPEETAILQQQLQQLSQFGTSPPCAFAASNTSTAAATVAAAAAAMAAASSAPYSNPYMGLFGGNPMAPAGPFGGPASAFAGVNAIGGGTISSGAFGSDVFGASSLASSAANPAAAANVGSQEVSVASLTSLLTTLAALAKQGGTGPAVPTAIGTASISPDAVLTGVASNSTSASCSDPSVPGTPAAKATTPIEPSSGSASSEAPTSASPTGVSIAGSDDLSVAGGPTAAVMLTQMTPTPAASPPSSTVGADEPVPEVPELVAPTAVVAAAAPAMKSPRGLALPGPINDSGSSAPSLAILQQALALLAVEVPVTAAAALKQPSPVSAQTNNVVVGAMATSLEASAGLCSTTLSLPGVTVTPHELQMARSLHAALKVVALLDSLSNNTPVSCQSTGFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ74</accession>
    <name>A0ABQ5RZ74_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_005075</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATPase B chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06503">
        <property type="entry name" value="ATP-synt_Fo_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01398">
        <property type="entry name" value="ATP_synth_b_bprime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01399">
        <property type="entry name" value="ATP_synth_bprime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034679">
        <property type="entry name" value="ATP_synth_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002146">
        <property type="entry name" value="ATP_synth_b/b'su_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050059">
        <property type="entry name" value="ATP_synthase_B_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33445">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT B', CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33445:SF2">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT B', CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00430">
        <property type="entry name" value="ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI62449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="210" mass="22706" checksum="5EAA53F99F22AF77" modified="2025-10-08" version="1">MASMLNRPQQAFVRAAAKPAAVRPMRLVVRAQAEKPSQQLMQLTKPAFSAVVANALLAMPAAAEAGKIFDFNLTLPVMAGEFLLLMVFLEKAWFTPVGKVLDERDNLIRSKLGAVKDNTGDVDKLVVEAEGILKAARSEVSSMINSQKNAKQSELDKAYNEAKAKITAEVETSIAGLEKESAAMLKTLDAQVDKISAEVLKRVLPEGVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ89</accession>
    <name>A0ABQ5RZ89_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45286">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45286:SF1">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="139"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="701"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="767"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="707"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="991" mass="103947" checksum="C6B872338F194019" modified="2025-10-08" version="1" fragment="single">CPFLPPCRSRRGQTRNGYSAFIGSSSSTERIGRLGVCHSSSDGHGSSRTGFGACWACSCPVLSGPSSSSVRSFAAGTFSGGSSAASGSSSGSRAASGSSGSSSSKRAGAAAGSTASGCSAHHDGVGGDPLLLGLIMTGNHYEVLGVPRDASLATIKRAFRQRAKELHPDVLIARAKARHQQHPHDRLPYTYDKRHSYDNTVEQSAAAFLRIVAAYEALSTPTRRRAYDMQLAAETAAAATAASGYRRGTDPKPAGAAAAQQQQQRASRDGRAAASGSGSGSGIGNTDLRHVDFRSWRYGIDEWMSRARREPVEGRTATLDYYLRKYSHEFESHLHAALVHAYLGPKLDLQPGELPRCFEADVRRHPGASDQLMQLVSGRTLLGVVRVKEPLRLESAAAAAAAAASMLPSAPSSQPPPPPFRSIRSEDMLSTIEGCEAEAEAEAGVEVEAKVEVRKGLRVESTAAAAGVVTAASAAADSIRTSGGLVDGYSGCSDGDGEGGDVDPYPPASTSSSSSSTAATAVAAVSLAGIYGNGGSDKKGGADPLDVLGAAGVRGLSDADVVHLAAASIARNDADAAGYGHLVPGYSRLRGLLECQGFYPDAAVLQRRSMQISLVTAAEAAQARNGTAALGGRRLMDGWRRDAAAAVHAGPIAAVGGREEQVLAEVTPPPQLQDLAEATRLAGAEVAAAATADEAATAAAAAACRKPPSPPFPPLNSPRRWRFGPDHEHGANSHDVGVVGGGGATKILEMWLGQNLTSFAVHQLPLSPSPPLQPSPSALGNSTASTKGKALDLVDPRVTNTTKTAPGDGASHPEGLMVPIFGPVIEGRIKVYINGHLEAGVEGCHVYDTEGRRVALVVRGSFPGLRTIHFFSIHAAPDTTQRPRLRLLCRCVRARLLPSPTWLFPPREATHDVGGWYFEWGGYNRRGQPGWLDPSVFVFVAATVASEQERIEARHQRVVSELLQGWWQAAWQALAGWWERAGWTPHEERKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S075</accession>
    <name>A0ABQ5S075_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006157</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045670571" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="33"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="769"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="725"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="727"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="921" mass="98550" checksum="F67B89BD02981DDB" modified="2025-10-08" version="1">MARLLLLTLGCLCVLIIQASGQSVKSDNPNPLFPDCKCERDERASPYRAYFTNVRPYPGGKRFCFTFDIVACQYDNPCCYMNFYKIELPFMLGCRDAVQFIEYQGGNKSVNWGPAYALANPHRIDLKITNMRLSQANTRGQSICIGIADKCAELSTWCWPASGNCKLAMFNADPFDCCPIQPFNKIVPAYRYPPPPPPTPVKQPPPVVVSPPPPPPPPPPPPPPPPPPPKKRSPPPPPSCNACVRSYLSYVPPGGKFEFSEEKCSELQYFMVDELNAQADALGARMVVPFTPSACSSFEVSVCGTFFSDADGARMQAYMDAGSLSTFNNFLIPANEPCPAALNGYYITTEVKDSPCLYGAASKSCIAMPPPPPPPPPPPPPPPPPPPKPKPPVPRPPRPPPPNPPPPSPPPPSPPPPLPPPPSPPPPRPPPPRPPPPSPPPPNPPPPRPPPPSPPPPSPMPPSPPPPRPPPPPPPRPPPPPPPSPPPPKPPPPKPPPPSPPPPSPPPPNPFPPRPPPPSPPPPSPPPPRPPPPSPPPPAPPPPSPPPPKPPPPSPPPPSPRPPNPPPPRPPPPSPPPPRPRPPPPSPPPPCEVCISITITSFPIPDHFRFDDATCTRMQVLMAKDISVQAAVVGARLLTPFAADLYECYPEEIRVCGVFFSDSDGQKLQPWISSSVPYINWYEALFPGSCSSHPEISGYSFKLWASNYDGGAACLTGSFIPPSCTTLPPPPPPPPPPPPPPPPPPPKPKPPPPPPPRARPPPRPPAVSFPQCECDKRPGSSSLTFSSVNVTELKTGMRMYCFVVGLTPCLRQSPCCSQDLSKIEFSVVPACVGSVAYSATDGALRSAQFQLRPYPAIKINTIAKPFIDVDGTTVCLLLRPPCDTLESLCGGPNCLYALFNNRLTNQPKCCPVRNVLDPLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0I7</accession>
    <name>A0ABQ5S0I7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="233" mass="24700" checksum="ABFE828A81695121" modified="2025-10-08" version="1" fragment="single">PPSPPPPPSPRPRPPSPWLSPPTKNAVKDAPFNGDAPPDFPVSPIQPPPPPPPSPSPPPPPSPPPPPSPQPQSLPPSPSPPPPPSPPPSPSPPPPSPSPSQPPSPAPSSPLPTPPLPKPPVPNPPSSRPPSPRPPPQRPRPPSPRTRLPPRSPPPRPRALSPRPRPPSPPLQRRSPPRPPLSPPPRLRHPPLSKSIPPPPRSSKPSPSRRRPPPAVRRPPPLQQRRPPPQRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0M4</accession>
    <name>A0ABQ5S0M4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Purple acid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PAP1</name>
        <name evidence="5" type="ORF">VaNZ11_005586</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051558">
        <property type="entry name" value="Metallophosphoesterase_PAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10161">
        <property type="entry name" value="TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10161:SF14">
        <property type="entry name" value="TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="4">
        <location>
            <begin position="88"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="425" mass="46786" checksum="2FFBC906952BF1DC" modified="2025-10-08" version="1">MGSQALRTDFELEEEDRFFISGVEKVGRSRNTYEHQLRKRKVLVGVLIAVCSLGLVALIIGLGWGLSHRKLDYVCPRTYDPGNTDLVFYVIGDWGRAGSEDQQKAARLMSDVSQCMPPKFVISTGDNFYPSGLSSPADPQFQQSFTSVYSAPGLQVPWYAVMGNHDYGDSADDATLTSCLSAPTSEKQCEGKCCISPFWQTVPELAARDGRWNATLGNVVTRTFLLGAASSASGSGGTLDILFVDTVPMIYQYESRRWASFLYGFKNQSAHVEDIKASLVRQLNTSLAGGSKWRLVVGHHPVRSYGSHCSQPDPNDCMDMAFLRPWLREYRVAAYLNGHEHDQQLIKSDTDPVYYVTSGAGSETRAGEFDNLDSDERSKDALFLSDKQGFLAVVFSGASMKLHFYTTGQSGPTYTRVVPAPSSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S183</accession>
    <name>A0ABQ5S183_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1470.10">
        <property type="entry name" value="Photosystem I PsaD, reaction center subunit II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053233">
        <property type="entry name" value="ABRA-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21715">
        <property type="entry name" value="RH04127P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21715:SF0">
        <property type="entry name" value="RH04127P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="154"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="442"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1023"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1127"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1366"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="697"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="268"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="487"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="572"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="582"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1144"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1154"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1369"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1428"/>
            <end position="1448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1480" mass="155723" checksum="C69D02E71AC64C57" modified="2025-10-08" version="1">MANGGEVMPDGQVVLEEEIDENYEPTEQEIHEYAQWLGIDTETEKELFWIAREGLKSPLPDGWKPCKTPTGDIYYFNFNTGQSIWDHPCDEHYRKVYQEEKAKLATKARQSFGTTEPRAGLLKRTGAETKVPSPLGLSSSKASDYILESPAYEQQGDLTGGNASSSSSDNGGGGKGDAKPVKHSSGGSGGTGAGGGWWGSSGTDAAKGKTGSQQVEQKDKATKKDAPMEQSSLKSFSSSASSLTAAMNELDLPDEGGGRRTGSGSKGGAAAAATSTNSQKQAAGKVSASGGGPRMTTSGEPPTPDLSTTGATRTTVTNAGDSTGPGTVTAANVGAAPSQFGDERLGQGTASGLGLPPGVTLLNAGSKGSSVAQSGGVVAKSTTGSSALRRSKKYDDEFADFDDLIDDDEIEEGVQDVGAAAGGRANSDLAPAASAKLISPMAATAGGGTLGGSVFGSRDGAGDLSSPAMLTHHRRYPSDNDNEDPHPSNQREPTASGSVSRAAPVQQQQPAQQQQQQTQQQLPALQMHRQASAQRPLPEPDPRPVDEQRHRGGGGTAAPVTSFGAGSRLDREEQEQRDEEEVMRRTATREADMRRREEERLAQLQEALSRQYEDAKAQLQKQVDAELVSERERLRAESMAKLQEQLSEKSLELGRLERELAAKKAELTDMEARTREQKRVMEDNQRELSSSAHTVALAAAETELRGKRTELSELNAQLLAKEGEFTALASSTAARAAELRDKTAQLDVAREQLAMAEAKRTAADSEYQEQQIRIRALRAESEQLSAQISATQATLSRLNVDLRNKEDDLAALNKQLAARQAELKEVVTELSTSRTALQSKEIQVVEQLKSAATLEYEALQKQLEAARGALDAARGQLATASAELTRTEAQCGALKTQRIQLEADVAARSAELDDMRSQLLSCQAELRSVRAEVAERSGALESVATQLLRRQKELADTDSMFAAVADQSKDLLELKRSQMQRNVEETVDLERRTLLATRMASMRAEVESQVATEREVYRNRLLQQQHQQQAQSLTHAGRGPGDDSPGGSNAAVNGNTSLQQLPPALTTANLAASTALSRATMPVGASTSTLGDDLRASTSIWAQPLPALSPKLPASLQPGSGGGAYANAAPHSYAKMPSHHAPPLVLPPEPQLPPQQHQSAPLPGSAAAASYGLQLQYGLQGGASPADLAAAQYAAAMAAQSAAVAAQSAHANLPELVSLAENLHRSLQLLLPLASQASKQSNSSSPSRTRHASLLQPGQVVLPPSAIDWTNTALPPAAAEDPVLSRAWVYLKLQKAYLKERSSILQAARNDWKEDLRAVMSTPGPGNEERLAVLQSAKAGLEEQVHRLNEDSKQLHRMREQLQALTAKTSGMGSGTNTGSSGVTTGASMATGSTAATPSSASAGRVGSNGQGSPGRGVSPQPLSAAGGAMGAAGAGSSGGGGVRGGGLGGNTSSNVDLARSLLQQHSAWLKSFAQQLSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S187</accession>
    <name>A0ABQ5S187_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006306</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="334"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="661"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="972"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1056"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1144"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1701"/>
            <end position="1785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1835"/>
            <end position="1905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1922"/>
            <end position="1959"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1353"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1443"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="347"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="387"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="441"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1182"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1211"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1238"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1707"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1753"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1844"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1883"/>
            <end position="1895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1896"/>
            <end position="1905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1944"/>
            <end position="1953"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2019" mass="216271" checksum="58EB254996C7D1D4" modified="2025-10-08" version="1">MTLEKGKNYGQEQIQQTLQDKARTRLNNPAPHQAPQRERVSRSTIPGGRPSSQPRNRSRRWKSLIRPDVNLGVPQLMMILEPNIVWEHTKGFVPGVGKEVEANVTPGEFFDGWHKGGRRAHGVYEGSYWELMGDCEELAEMLRKLSKAPALLEEPPLELPPPPELPSWDDFKPNLHLIPAAPTLPVLKLPPFRAPILEPEPPFVEPEYLPLPPVPPVPKEEPLPIFIPPEFLQVEPLVEPEYVDPAPPLMPDAPPPPSYGQPKHGHDWSGLRPFQLDLPSRDAFLAEASAQRVMDTSRFVHPPNSLPVVPELEPLDEDLLKGLDLEPIPRLELAWMGGPEPDPPFSSVPSSPAALSRSTSRAPSRPMSAAPSRPMSAAVQSGSPLEAAGNNATAAASTAATPTRPRASSAASQRKLTNDGSTARPWSAGPRAGSSTGDGEAAAPAATAAPAAAPAAALPPPSPRTRSARRGSSMSRTPLAVVKTPEELAWEEARSVASEASGTPRLWMNFSAPFSDLPPIMMPPRPEPGPEPMPEEFPHEQLEDLPVLVAPPLLEPPVYVEPPQFVPQWAPPCEPRPVGFLRGTAPVLICDVSGTMHPRQQGRFRHLKRCAVDLLDPEGEIATAAAGFDVITFAGGAWSWSAGYGERMGLLQSSQVPYSGRQVGGKYSSKTPPGAGGAGAGSRTGSPDRFRAGASSASGSLALMPTQPDLLSDAQRWVANWPDAVGQTNMTAAFALAEQHAEADCWYILSDGMAHDQQACLEYLARRNEAGNLPVIHAVGFIPADANPDSSGERFLRGLAELTGGSYQRYTPDIHKVWVTGQGFQRIDLRNETPEQREERQWAEKQLRAERIKNLRLGISEPLEVTMERVAVLHQQLRVAPATRANEMALAEARRRHAVAMESVRLTNERRLAEATAAYDAAANAVRARNQAKVDAAREAFLQAHGDWERDYMERLQDWEDAKAALQGQREEQLAAARGGAPVALSPRPSTSRPYVSISGGSTGSRPTSATTYAALPRNKAGSGLLPLNYEPSEHRAAYAVAEYDTVMGLDRVADKLSSPVHTPPGSRPISAIASGAHSGGGGGSRPVSALKAAAAGGGGGGGSRPVSGNAADAAAAVEVAAGGASEYLEAITEDMAEASAPLDAVYGGGEDDGLDVRPSRSRSGGRGRGGRDQDGAGRRSTASQVTSQTPSSRPVSGRMASAGSIGRVTSARSLSSSAPESRKPTGPGMLPEGSMLQPPPLLPEPTAPLQPLRGEKSDEFKRRLLPPEVVEEHDAVNTSRLDDIRQQWESLVVADLAANSGRKLAAMRRNAGRVEEYRKAVYEAYANGLIRDVVYGGAVRVAANMVLMDSAEARYLAEVERINAENARLRQLHATELEERKAKEGAHANAIRRWEETKEALARQHQANREKARAEHEANIIRLKAEWRAKTQQVLELNDARLTEAEAMHEALVEQVKDTNRRHKDDHTELLRQRKEVETTNMSRLAEAKAVHKVRLEARVAENERLIKTAMEEHQATCIRLQEEYEEGKAQAEVDHAELCAFLKAANEQLTEEARRRYQEALAGVQAAYEADCAAVREAHSRDLERVKRFNQDIWPKVHVARLAQAELGRVQVFAEHIRHCANKFELGINLMPNTPNFDRVVEMQALTEALGKAFPDMPLQLLPWPEHERRFEPGTGWTASPPARSLTYRDPMDIINRIAKGKTPLSANSPASGSSRNESPAAQRAQTSSAEAEAGVLSAAGSVDDLPHPHQSPQQTYLSQLQQQQHHQQPSSNGSPYTPRHISRPLSAGRARFANVLNGDLQRPLSAAVPAPCSARVEAWGNAVAAAASALSSPGSALRPQSAATAPYQNPSAWRRSNTTVLRAGSPRGVEPRRNSSNLGPGVGGGGGSGGGPPSTAGGTSMSQSAAVAAAAAATAAMGGLTSRPGTARPGTAHGGGGGSAGATRPRSAGPAPRPPARVAVDVTSLQASVARAKAQAGTHALTAATLAGFSKDVPKLRYPEVDVPQALTIASSLRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1J2</accession>
    <name>A0ABQ5S1J2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006809</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051860">
        <property type="entry name" value="Plasmodium_CSP_Invasion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44826">
        <property type="entry name" value="SPORE COAT PROTEIN SP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44826:SF3">
        <property type="entry name" value="SPORE COAT PROTEIN SP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08284">
        <property type="entry name" value="RVP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="405" mass="41823" checksum="100DED3A0DAB22CA" modified="2025-10-08" version="1" fragment="single">LRGYHIQHAASPAASPAASPAANPVANPVANPAASPAASPAANPAANPAASPAASPAANPAASPAANPAANPAANPAASPAANPAASPAASPAASPAANPAANPAASPAASPAANPAASPAANPAASPAANPEAVPEAEPEAEPEAEPEAEPALKLSATQLASMVCNAAHQQLAFKGTVNGRMAVILIDGGATGSFVHTQWLARREVKAAKTVNNMSVKLANGQEQDCPYVLPNASVTIRTFKCRSTLVATDLGDFDVVLGKDWLAQWNPHVCWRTNTVTLRTTLTKHVLRGDPPEAVNERAPKILSALQFKRVVRATKQYNLCVVHEVNTQVQEEMLRHYVDANHTNWCDLLPQLEFAYNSSVQASTVFTPFKLVLGYEPTTPLPLLAKHPNWEPATAVFANVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1P7</accession>
    <name>A0ABQ5S1P7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">von Hippel-Lindau disease tumour suppressor beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006379</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VHL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05468">
        <property type="entry name" value="pVHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.780">
        <property type="entry name" value="von Hippel-Lindau disease tumour suppressor, beta domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024053">
        <property type="entry name" value="VHL_beta_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037140">
        <property type="entry name" value="VHL_beta_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036208">
        <property type="entry name" value="VHL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022772">
        <property type="entry name" value="VHL_tumour_suppress_b/a_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01847">
        <property type="entry name" value="VHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49468">
        <property type="entry name" value="VHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="von Hippel-Lindau disease tumour suppressor beta" evidence="3">
        <location>
            <begin position="35"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="458"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="169"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="508" mass="55457" checksum="1AF08D202C37216F" modified="2025-10-08" version="1">MGISHSLDRLRAAMESTAEQPPNSRTWLFEDGDKVSSVNSRFAVNMCYTNRTNHIVEAFWLNFEGKEISYGLIKPGATHLLYTFLTHPWVFRKYGNHEEVLTVHGKPVAWPNQTHPFVDIEEAPIVPWNMAAHASTFKSVDPVFARDVQNLLRAYYIQRKANGRSSGVKGCRSGTGKSRRSSTGGMLDAEASPTKGGVPCCGVKLWHWRGLENCNSLQTAPTIHATTRATVAVATQLSAGGCPPFAPLDTLTYPLTLSQSCAQLQPEDGLIGKLPFDIILEIIERMAPPLRNYKAVLKDDIEMMPRGVEPDKGPVAFMASRLLGPIPPPAPLADPQVWFAAVNNGAHAAAVPEGFNDLGAPMVGNGPLLPQQQQMQQQPDNAQTRQVAEVLLYVEATVLANAAMAHSYQMEVQQEVQAAALMRFLWAQVSAEQTLQQQQQDQQDDGGRQGLPQERDGLAVVDDEQGVGSQYGDAVDDRELEHRDDAAGAQVGAAESDDEVFQDANAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1Y2</accession>
    <name>A0ABQ5S1Y2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">EXPERA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_006966</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007905">
        <property type="entry name" value="EBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14207:SF0">
        <property type="entry name" value="3-BETA-HYDROXYSTEROID-DELTA(8),DELTA(7)-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14207">
        <property type="entry name" value="STEROL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="7" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="15">
        <location>
            <begin position="56"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI63856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="223" mass="25386" checksum="CDAFDB3083DC0D15" modified="2025-10-08" version="1">MATVKHPYVPHDLELPGFVPNVLAYETILGVFFGSSALVVLLMFTITGRYKYLTTTERLLAGWWMCTGIIHFIIEGNVVVNNKYYQDSTGHILNEIWKEYTKADSRYATRDAFIITMEAVTAFAWGPLSFLIVVGVLNRTPWRFSAMILVSLGQLYGDVLYFMTCIYEGVHKFSRREPLYFYGYFIGANAIWILVPLLCIIYCSKNINSAVAKSGKVEQKKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S217</accession>
    <name>A0ABQ5S217_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006885</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="174"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="365" mass="40260" checksum="6430D8C75AF43EC0" modified="2025-10-08" version="1">MTCSKAHANQQAVPVIDLGLPEEEAATQIREACTGSGFFYVTNHGVSQSLVDDTFEQQRELFHLPMAEKMAMLQNENNRGYTPFREETLDPVHQAHGDTKEGFYFGREVAADSPESKKPLHGPNQWPDPQLMPRYREVTWQYYEALYGLGLRLLRLLALSLGLPEAYFAPMFDRPMVTLRPLHYSGEVSDPGAGVFGAGAHTDYGMLTILATDDTPGLQIWTGPDSTSGTAPNSSNGAIGAGREGSGAGDDGTAAACGRWWDVEPIPGTFIINLGDMLERWTNGRYRSTLHRVINTTGRERYSIAFFFEPNFEAEVEVLPVCCGPDEPPRYPPTTAGQHLLDKYAATHAGYHGPVKQGHEKQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S284</accession>
    <name>A0ABQ5S284_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="16256" checksum="3029421E8F91D37A" modified="2025-10-08" version="1" fragment="single">VAPGGLPLPPLSPAAAEAALLEATPLARSLAEDEAAGPGGAAVLRSAAGGAVRDPASSVPPETLAFLRRTVLAVNEACRHFWRSLPASTPARKEKASRLAKLLESKRSEVEALNDRARGVEGRFIRQLLKPPMDMLLAALVRWDEELQKRGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2F8</accession>
    <name>A0ABQ5S2F8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_007244</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_B">
        <property type="entry name" value="Ribosomal_uL1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005878">
        <property type="entry name" value="Ribosom_uL1_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01169">
        <property type="entry name" value="rplA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="302" mass="33215" checksum="517C954DD77DFD93" modified="2025-10-08" version="1">MATVGLHQRLLQPTAGVRRCPAPFRPVVPSRLQRLQDNVVHAAAVEEYEEVEVAASNKQQNVRREKPRSKRFKAMQTRTPGRTTELEPKEAIKLLKQTASTKFTESVEFHARMGLDPKFSDQQLRATVSLPCGTGKELRVAVLTQNDNLRLAKEAGADVVGGDDLIEKISGGFMEFDKLIATPDMMPKVAKLGRVLGPRGLMPNPKAGTVTTDVAGTVKDFKGGKVEYRLDKTGNLHVLFGRADFKEDDLLFNLKAVQESIDANKPPGAKGVYWKSMYVCTTMGPSVRLSVSAIQNTKGKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2M1</accession>
    <name>A0ABQ5S2M1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_006544</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0229">DNA integration</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="12">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="281"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI63562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="691" mass="74842" checksum="B0D2B2F6A88BE686" modified="2025-10-08" version="1" fragment="single">MLDDYTGASLVQPIKEKSDVANFMERAITMLQVQSNGTLQCIRTDGGGEFVNRQVQSYCASNGVRHQLSAPYTPQQNGKAERLNRTLLDKVRAMLAESAVPKEYWHEALMVANFVRNRSPVAGKPKTPYELLTGVVPDVSLLRVFGCVAYAHVPKGKCDKLDSRAQKGTFLGYEANSKAYRILLGDGRIQITRDVFLEDQFAFSNHVRPELEVFSIRDGPSVPVAGAAAGNAGQAGGAGVVDAGVPAATHAAGAPAGNAGVAGVGATGGAPVIPAAGAPAGDAGVAGMDDGEVPPLMEDDDEGEEEDYGMSETEGGGDYGDDAGGDVASSSGSETGQGSSGHTGESGNLDPTPPRPIRQRKPPGWQKDYAMLASELEMEEPKSLEEAQSRVDWPQWKAAMDEEMASLQENRTWELEEIPMGARKIGLKWVFKLKRDADGNVERYKARLVAKGFTQKEGVDFGEVFAPVGKYASFRALLAVAADRDLELHQLDIKTAFLHGELKEEVYTEQPPGYNLGGSNVVCKLRRALYGLRQAPRAWYIRLRKELESMGFKPSLADPGLFVKIENGEPVYVLVYVDDLLLACKSMAVIGQVKAQLLRAFAMRDLGETRFYLGFEIERDRTARTISVTQRRYISNILEKFGMKGANSRSTPMDANLHLTAEGDPLDTSVHPYSEVVGSLLYLAVCSRPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3A6</accession>
    <name>A0ABQ5S3A6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_007443</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12108">
        <property type="entry name" value="Hr-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd16464">
        <property type="entry name" value="RING-H2_Pirh2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037275">
        <property type="entry name" value="Znf_CTCHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF0">
        <property type="entry name" value="AND RING FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08900)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14599">
        <property type="entry name" value="zinc_ribbon_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161245">
        <property type="entry name" value="Zinc hairpin stack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="7">
        <location>
            <begin position="1245"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="8">
        <location>
            <begin position="1314"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="1381"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="791"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1041"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1044"/>
            <end position="1066"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1487" mass="162669" checksum="FAD1AFD88BE72376" modified="2025-10-08" version="1">MAGASGQFAPTSAYATSPVYVSALTRFPPINFIYGHFHNSIRAELSILADRVRSLEVPTEGVGSMLSDLRERYKFLEQVYKYHSAVEDEVLYPTLDSKVPNVTRAYSIEHQDEEILFEQLSQLLATALEESESKRKGTIRALICKVEEIHTTLRKHLAKEEEQLLPLLLEHFTFAEQAELVAQFLYSIPLETVERVLSQLKPHIPRDEQEQLLVEIRKVIPDNLLSQLLMTWLRPAADMPTTHASISGTERPAGLVCCGNPSACMYSPQPTTSPSLAPAVSSANEHVEQREQRAAYRTPSNGNVVYVAGPVMQDGAPYTAAEQLPVMQQVSRAPPSAFHHFRIPAVAASAAIGSAEGSDRAIGSVPLQDIIHFHRIICSSLADFAQEARALQLGRGVTAAHLSALLERHRFLRSVYVFHSISEEEVLFPEVQRLATNVGGMAAKQCEKDHQAELSSFEDLGRMLADVRSFARRGRKEVASMLEKLCCSAEAVHKSIQHHMQREETDVFPLLEAHLCEAQQRVLVYRTIRAMPLRLLERVMPWVVSGLDDAAAVSLLSNIRLGAPASDRPLVELLSRWAWRGRPSMAPFCGPGHGDNWAHVSRPCVGGDALDTCGPRLLHESFPGGSASHYLATTTDEGGAVRKRQRVSDESAHMKVSGSFGGDLRTAADVVFNAPRAGAEHGAHSVVEMDLSTAPRALMRGPEESGTTVVATPYGSERMPHDGQAIGGPRECTCDGGATNVSGSVGPYNPIDHIFQFHKALRQELKQMEADAMHLEHVVLTWVRHHKQVQPQLSSAPVGPGSPEAEESLGQDGDVHPARVQAVQSVGTLVRCAQPIALLDPGLTGPLDSGRVATARGGLLPVLQNFHGRFQFLQGIYRAHSKSEDEIVFPALEAKQALRNVSHAYTLDHRQEEQLFADLEAAIDKFRAVDLLTSGHEEELSRHVMGVRRMCAAVRASLETHIRSEESELWPLFTEHFSREEQQYLVGVIIGRTGAQVLQALLPWVSETFSEEERDAMMDSLRAATRNTMFDQWLEAVQGGTGAGASISSGTSGSGADSGAPSRASSIGVGDLGAVHASQQPLQDVAEYLAGGGSGSAAALDGDTSRALTIVSGGPAPGAMADSMSFRPGWEDIFRMNQQQLEAAIHRVSNDSSLEPERKAYLMQNIMVSKYIVAQQRRMGNLNIEAGRQLLHRQENAPRTLGQGTAAAGLTALACAGVAVDCTTSCAAAAVETADSVIHHSFHDATAGVLGCKHYRRKCQLVAPCCGKIFTCRLCHDDACDHRMDRYAVSEMYCMMCGMRQPVGGTCCCCGAAMARYVCNICHLFDDEPGKDIYHCPFCNVCRRGRGLGVDFFHCMNCNACMSLTLFSSHKCREKCIEGNCPVCHEALFDSSQPIKELPCGHFMHSSCFLTYTRYNYTCPLCCKSVGDMSVYFQMLDSLLASERLPPEYGGRMQQVLCNDCGKMGFAAFHFVYHSCPHCRSYNTRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3S2</accession>
    <name>A0ABQ5S3S2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="183" mass="20303" checksum="B22BD2637D122022" modified="2025-10-08" version="1">MDWWTNVSDTQLPSWQGPVGVKDDGGGCVRGCGRVGEMQLHRAVCNHNASLIERLVVGEGADVNEVEAAGNTPLHNAAFEGWEEGVALLLRLGAKVNASNNAGDRPWHWARNMGHTAVMELLERHGANKERGKVLVQEHVPKVKDFFAKHCWAHHPKPYADFITFKKSEAATLEAERKRVIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3X8</accession>
    <name>A0ABQ5S3X8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007917</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12368">
        <property type="entry name" value="RRM3_RBM45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034207">
        <property type="entry name" value="RBM45_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="10"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="310"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="394"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="501" mass="53367" checksum="A69A27DBDBF9D2D8" modified="2025-10-08" version="1">MESVDSPPGSRLFIVCGKNVEAETLQVAFRPFGTVQNVKVIREKGVAYVKYDKASSAALAMENLNGAVLNNGRGPKLKVLLAEAPNTSRGMHPPRPAEQEISSDPDNIPPRSRLFIVVPKQADPQHINESMASYDGLEYCKTDLVASKGVVFCKFTKASFALRALEDVASRGTVGPYKVKCMLAEPKTKRGRGDGSPQDMFSPLTQATKLDYGINHHLPPDAAHMPLDLPMGHALSPSPIGVTDYTTISGLGGLGGLSGFNHLGDAANLANMQSHLSAQVGALNFNMGLSGLHTNSSNVTPHDSPTLSKQRLFVVVHKSVTQDVLARLFRKFNGMEYCDLKKDPATGRSKGFCFVNYSTPEAAATAIAQLNGIEFPPHSNQRLKVMYAEQLGVRSTNGPGSASHHSHHSAHTAHPVAAAQGARSPMAMSQLASTPSPVHTPLSPAVNLSPEVNVASVQETLANMSMPRVASANGVEELEAARRMASPISSVARELMFNVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4E7</accession>
    <name>A0ABQ5S4E7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclic phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012386">
        <property type="entry name" value="Cyclic-nucl_3Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141:SF1">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07823">
        <property type="entry name" value="CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="31771" checksum="84A37F5E881A633E" modified="2025-10-08" version="1">MQLHARGHFGPPSPSVHTRVPVHTPRYAGWGLLLGLALLGLGLYWYGSALPHTRINQQESNHLSNYHLLPFQLYLRRTAAPLSVCSRSASPMASPSVSTVPSSALKESYSIWAMPRGKLGDQLRGEIAHLAQRHGGPAFPPHVTVLGDIERPREEAQALMKELAAQVKKYRINFVNVTRGTFFYQCVFLLVAKDEGTMAAAAAARSVYGMTTPPYMPHLSLLYADLDDTEKAKVVEYETARLYGESSGYDTLLVETGYEVGSLALWYTPTDDETTQSWCLVEEYPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4N5</accession>
    <name>A0ABQ5S4N5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007735</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09212">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036339">
        <property type="entry name" value="PUB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143503">
        <property type="entry name" value="PUG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="403" mass="44390" checksum="BEC813CDB0D8FEBB" modified="2025-10-08" version="1">MTFLQATVQVTDATAVWLGTGTNDGERRALLYALCSICNLIRFHPIPLENPLGKPSTMSNLEAVLVAKGLNADVARACSHLFGSYEEAFPYIDPPVIPGDIKEYYSLKYHRYYYHCTTSDTTTWELPPQLQLSEWMLDASISGQDVRTCRKLLSALLWAVQANAHKGAALWSALGLLGRLTGNIVSCGNTVEKYRTVNKSNPKIREALQVVHGGEAVLLAAGFVERPVGGSTLVFPAPGPEGEPDMRPLMAINSKIHQVVSRKGFTGMSRDSDDTGSTTRSRHAGLPGFRYQREIVECSCCGRPINDGSDRLVTRKHDAPRGEFRYECELCSGYNLCEECWDKFMSGTLVPSHPREHAFQTHHPKASQHNLHSNVSDSNPWGVLVAGGSAARARERLKHRTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S510</accession>
    <name>A0ABQ5S510_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="3">2.5.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_008337</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = N(6)-dimethylallyladenosine(37) in tRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IPP transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF60">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="105"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="567"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="569"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI64936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="633" mass="67192" checksum="580EFCDDE3C2318F" modified="2025-10-08" version="1" fragment="single">MTAGGSRVTHVMLCSSAVVATSSTMTGSLALGAREAQYEETQDDLSPSVLPPQLSSQQSLTVQAETADPVSVLGSGATQPRPLVVIVTGPTAVGKTAISLMLAQRLTQPQRQQPERQGVTDRGAGDEVTGISEGSHAEAGGSDVDYGREDGGLPTCPRGEVISADSVQIYRGLDVGSDKLPPDERRGVPHHLIDVRDALEDFSAGEFFECARPVLEDIVRRGGVPLVVGGTGLYLRWLVVGKGGAPKAKPEILAAVEAALKTAWEAAEAEKRAAANAAAATDTTPIPTTRTETPAGVRGDGCTEVYGACTASLVENPMAAAVGTSCLTEDDKWEAAAALLLKWGDEVAYERIRAERNNYYRLERALSILLMHPGSRLADFEPRADPNSGPDFDFRCFFLHRPRLQLYDRIAARVEEMILGGGLLEEAAMLLRRGLTPGSNMAAKAIGYRQAMEWLIAAKAAGRPVGLADLCMLHGAICQATHKLVRSQMTWFRDDPLYKWIDVDGRDPEDVVAEILREVAKPRHEGGCGDSGRLSKEEQKAVMRYKPQLKLLTDANAKAAATLAAAAPVAPPPPPPPQVMPTDPDLPLSLPPPPRPVKPKSKAALAAEAAAAVAAAAEAQLAPLLARINEELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S543</accession>
    <name>A0ABQ5S543_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cas1p 10 TM acyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_007922</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. CASD1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012419">
        <property type="entry name" value="Cas1_AcylTrans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13533">
        <property type="entry name" value="N-ACETYLNEURAMINATE 9-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13533:SF1">
        <property type="entry name" value="N-ACETYLNEURAMINATE 9-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07779">
        <property type="entry name" value="Cas1_AcylT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="303"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="483"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="533"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Cas1p 10 TM acyl transferase" evidence="10">
        <location>
            <begin position="117"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI64613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="559" mass="64476" checksum="827CEBD874974278" modified="2025-10-08" version="1">MALLTAGISSGQVTLFVAFIWSIVAWIYAEYLHSRRAPAKHGDEESPRKEEAAKLLSNGSSSESAALSSAHGNGTDSSNGNGTLEESTHVSKAYGVHSNMWESMTNSGLVSCLLLKQQAIEQHRLTLRAIVEFGLLIIWYFVADRTTIIPEGEKSYSRDVFIFLFVVLTSVALGTSIRAFKMPLLLNRPQTEEWKGWMQVLFLLYHYFEAKEVYNAIRIFIAAYVWMTGFGNFSYYYKTGDFCIGRFAQMLWRLNFLVFFVCVILRNSYMLYYICPMHTIFTVFVYIALAIAPQLNSNHGWLFVKILLSFLFIFLTWDLKFIFYAIWSPFKFVLGYSDPRNPTDDTLHEWYFRSGLDRYIWVYGMLCALMHPKASAMLKYIDDLPTLRRYTARTIVLSFCAVAGYVYYITIYCKPKYEYNEVHPYTSWIPITLWIVVRNITPWLRVHSMRLYGWLGCITLETYISQFHIWMKSDIPDGQPKSLLVLIPGYPLLNFALVSALYILVSHRLFDLTNYLKNVAVPHSNNALLLRNVILMVLTSIVLYTLVTAIYIATGKIWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5B4</accession>
    <name>A0ABQ5S5B4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007733</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="6">
        <location>
            <begin position="29"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="185" mass="20952" checksum="FAC53304F3194400" modified="2025-10-08" version="1">MLKLFSVKDKQKQQQEANAGVGKPGKLSAGELRIQKDMAELNLPPNISINFPDGKDKLMHFFISIRPDEGIYRGGKFNFEFNIPTGYPHDAPKVLCTTKVFHPNIDLEGKICLNILREDWKPVLSISSVIYGLQFLFLDPNPDDPLNKEAAQMFVDNPRQFEQYVQRAIYQGAMINGVHFPACTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5K6</accession>
    <name>A0ABQ5S5K6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="40"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="366"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="528" mass="56415" checksum="7E0DA655C121BA17" modified="2025-10-08" version="1" fragment="single">LCLYSDIDIMKLGALVVLGVAAAFAVAHAQTTDTVAYPNFPFCQCIKTPSPYSILPVVRNPSKGTYCFTLNVTKPSPSATGYCATQADVYKIEFNVFKSCDVFGPVVNATLNGKPTKVGPNFDSPAQGPEDARILRLTQLDLGLNSDGAELCLSLGLNNRSRGCATLEDLCVPPTGANRGTCSVALFDSKTNCCPISKPSPPSPPPPPPPPKQPPLAPPPPENIVPFIPCRTCIYLTVQEVTPHLWPFTFSNDICDVLIPEAIELVQTAAQNAGAGLLSNLTTFSCDAAVINICFAFTSPNALQGNVSDILVAIRDLLTPSCPAYLNGYLVKAFVSDYGSDPFGDPVESCLSGIEVKTCSPEKVDFPKCFCDTKALSTRFAALPYLTAAPGRPTNKVNSTLYCFTLAVVNPSPANTYCANTTVLLKAELWGNDAPLQRRKILALAFQEGNSNTLKYISPSWGANGDQTLKVSPLNWTTEEADGAKICLELSNATNLETFCNHGDKTCWINLFSPTKECCPVFAASLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6I1</accession>
    <name>A0ABQ5S6I1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="175" mass="17836" checksum="307B04FA7025E99E" modified="2025-10-08" version="1" fragment="single">VLSDKERILADIAASPGGITAFNAAMRCALLLRPLDYEGELAAALTEPAPTTHGPVPAAASTAPPASQTAQPQNWDFAPLESWLALPPGHRHHRALALLGGTGTGKSSAAAAAMKRLMGPASASSALPSASASSGSWAGAVVPVVAHFCRARNLQALDPLAAMRSLAYQLSLIFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6X3</accession>
    <name>A0ABQ5S6X3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Diacylglycerol kinase accessory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009276</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF54">
        <property type="entry name" value="DIACYLGLYCEROL KINASE THETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Diacylglycerol kinase accessory" evidence="7">
        <location>
            <begin position="19"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="198" mass="19965" checksum="D9040577AFDA7A84" modified="2025-10-08" version="1" fragment="single">GGGGGSNGDSSGRGQGPESFSPDGHVGNALGVSVPASGTATRRSGGYATAIARVPADLMIPPPPPPPQLHPEAPQSISDGVLEVVVVYGAVHLGQLQVGLARATKLCQCRTAIITTHQALPMQVDGEPWMQPAAQLSVSLKGSAVLLRRLDLSSATSRLTAAVGEVLDGAVVLGTITAVQRQVLGAQIAQRLGAPHAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7A3</accession>
    <name>A0ABQ5S7A3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009069</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046932654" description="Peptidase M11 gametolysin domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="3">
        <location>
            <begin position="133"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="540"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="873" mass="91889" checksum="D01AE8435BD0126A" modified="2025-10-08" version="1" fragment="single">MASRYRSSWFLSCILILMYMLINLQAQPSHANGEGTIAGTILILMASLMNGEDTTQFYLQADGAQLYRLSFCDDVPLGEILPNSHVVISYTKSEGGVLYSCKTPQPDQDRRVLFGNTMTTPAAPSFLVYVPKFCGYENSVMPTYNDIMNFFSGSTPQGRNLLGYFKQCSYGKVSLGRITVINNFTIPCSGSVELSFTFPTGNNFDTKSCNNDNMLKWHFYLDSQMDGDDLPTDFHHKVLLLPKTFTRLPGCNGIAGSASVGPWLRQYSPVNNWGTGLIWWSGDSFGDVELLFHEVGHNMNMAHANIPGGCDLQDQCDDTCPMGGAGGQGVRCLNAPHNWQVGWGRPFLDLSDNSDTSLRPGLAQNVTIPSQMSVPNSSLTVRFNNMLVNQRLYISVRINTPPYDLPFSKDKNNVPFVLIHSYSGTATAQYVRTVKLGMIERGSKFIDSSGLVVRFKDWDGDSATVELCRRIGATETTCGDGLDDDCDGLPDSKDGDCDGAGSDITMVDTGGNPSLGGTATGALAPPPGAIRTPVRSPFLRRPPPPARKAPVVRPPPPPPPPPPPPPPPPFLPMFPSPKRRRPPPPVKRPPPKPSPRKPPPPRPPPPRPPPRFPPSPPPSSPIAPSFPPPQLPEVPSAPPPSPSPLNPNVPSSPNPPPPPPPPPSPSPPPPPPSPSPPPPPPSPSPPPPIPSPSPPPPPPPPPPPSPSPPPPPPSPSPPPPPPSPSPPPPPPSPSPPPPPPPSPSPPPPPPSPSPPPPPSPSPPPPPSSPPSPSPAPPSSPPPPPSPPPPPPPSPSPPPPPSPSPPPPPSPPPSLSPPSPPPPSSPPPPPSPSPPPPPSPSPPPPPTPPPTSPPPFPPLTPPPPPPQTPPPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7L7</accession>
    <name>A0ABQ5S7L7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009466</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="138" mass="14607" checksum="13550E7403D57BD2" modified="2025-10-08" version="1">MGSSMGSSPTTFPVVEGHHSRKQQGCTAIQGAGFACGGDPLLGAAEPLSQAQVSIFGFIGRHNGKRYAGRAAGPPRLFYMEAAIQLLCAELGYSRTLLHAPIAEADVIISDKVKNVMAKNVKNHHHQTIVWAVNVKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8B4</accession>
    <name>A0ABQ5S8B4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009144</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07505">
        <property type="entry name" value="HAD_BPGM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848:SF4">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="4"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="114"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="80"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="421"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="426"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="443"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="706" mass="73798" checksum="B96AB3A2E85FEBE0" modified="2025-10-08" version="1" fragment="single">MEYDVSEILPDLYVGGLSSLKYLEALGITHILSVINFPIQKDISAYVTLQLPLQDSPGANMLQYLPAAIKFLDGALGSNPPATATPATATPATAEGLGEPRNGRSSVGGDGSASGRCRVLVHCQAGVSRSPAVVAGWLMRRRGLSADEALRLLVSRRSCADPNEGFRAQLCLFGDMRCSLDPEHPVYRMWCLEELAGQWEDQGFVDPVAFGQVPEGPAGLSAALAAQQTLYRCRKCRQLLATSAHVMPVEAALGHRIFRDRQRYRQTLAAGGGVTATGGDSAAAAAANADTSGAAESCLFLEPMQWMSSTITGVVAGKLHCPKCSARLGSFNWSGISNPSGAWVTPAFQLHLSKLDTVLPKPLEALANVRHPLVGPMRSAAAPAVMAAAASAAVGTPVAAAPSLLVTLPAAVRRLGLGRAGAREADEGEEQLPEPNLDLDPDATNSESNVETNASTQGSLDVSIGTAAGPGGEVQVCSIGGGGSSGDQAPDPGDGEEGRSCRTAWFTHLIFDCDGVLVDSEAASCEALRRAILEVTGFDIPHLFPQDYVEVFGMDVRSCVAHYRGRFSRGEWEAPELLAPRVQAAKEVHYKNLTAGGIAAFDGAERLIRRALDAGMQVGVASSGEPQKIQHNLSSSGLAPLIPRHAVVSASHVAAGKPAPDVYLAAMKVTGCLDPRRALVVEDAVNGLKAARAAGMFTVGITNQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8E2</accession>
    <name>A0ABQ5S8E2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009553</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434:SF64">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="7">
        <location>
            <begin position="73"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="324" mass="37019" checksum="47C3FB1162633EC5" modified="2025-10-08" version="1">MSMMRKALNLPSFLFSVFIFYWSLPLFAIIYRIRFINVGKRNDMLDWAKALVAFFRVTVLKQGEMTLYRGGRCLYLCNHRSWADFFIDTYLTEGRAALMSRWLVYFVFPVFCTSVIILRGIVLFKRGHIANKEAFNAWLDATLARSTVPGLLVYPEGHRSLKPTSLPLKRGMLHFAYNRKLPVQVIVTRGKEEVLSEKTLSARWGRTLVVTFSHVLKPSDYDSFDAFFDDIQHKWDACWKSSYDKPTAGVPELSMKDAHEYSYPRSMWWLQLGMTFLSIAILVGVMCGSWVGLRAGLAVLGSAQQPVVLGSAAWIGASMLRAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8J3</accession>
    <name>A0ABQ5S8J3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_009632</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130">
        <property type="entry name" value="CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130:SF171">
        <property type="entry name" value="OS01G0895300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047519883" description="Cytochrome b561 domain-containing protein" evidence="10">
        <location>
            <begin position="24"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="975"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1015"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1056"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1095"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1122"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="11">
        <location>
            <begin position="306"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="12">
        <location>
            <begin position="942"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="192"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="470"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="667"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="900"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1159"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="240"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="470"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="672"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="905"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1168"/>
            <end position="1179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI65951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1179" mass="122959" checksum="B988E408479647AE" modified="2025-10-08" version="1">MNFLTSVLLVTSALIFSARLASPYPSYWYNALEVIGQSLPNGPTQCDSHPSEAVTEPNVNSPHPGGPTTDGSIAFAFRDAQSGVLTTKLCPGKINKLLLSFSDPRLALLTANTTSVAFSSPKSPTIGCPNRVDLGGSVSFRAATAFNVTFQVSCSLAGQNVMFEVTSATNVKPTPWKQNSVNIPVSLGADCGTCASPPPPPSPIPPPSPSPPPPRPSPPPPSSPPPPPQPQTAASSTSPSPSPSLSPLPPPPSLPTPPPPLLPPPPAVCTASSLGYQCMRQMGKVLLHWSVNTSSAPAGNGCTPADPVNVNGNDIAQYGTLHIAAQANVTGYVGIGFGEDIEEMAPADIVFGWASGADGKAFVGTFYTNETEYLGMNVTWPPAGDPSWAYDTGVTQRISASEDGGDITTTTICFSRRLAAEEALTSPSIDTDIPTVIIWAVGSKSAFIQHDPDDVGGFVLTLNPAMVTAVPPPPELPPPPELPPPPELPPSLPTPSPPPLAVVGTPSPPSWPSPPPLAPSLPQTKNCAPSTLGYQCTVNKGNIKITLHWSINSSTAPANPCTPATRTTLTAADLAKYGTLHLAVQANIQGYVAIGFNSDPSSMYPSDIALGWAGSSLNTYYASGAALSNNDLTNPSWAYDTGVIYNSSTGITTICFSRRLVDSRAKASPDLRSATGSSNSNSTSVGGRRRSLATSGSVAQLGLIWAISPAKGLVQHFSSNVGGLFLNPTLEGTLPASENKLLQPTSLPAPVAPPAPPPGTSCVPSTLGYDCVATRDKITLHWSINSSTAPANPCTPATRTTLTAADLAKYGTLHLAVQANIQGYVAIGFNSDPSSMYPSDIALGWAGSSLNTYYASGAALSNNDLTNPSWAYDTGVIYNSSTGITTICFSRRLVDSRAKASPDLRSATGSSNSNSTSVGGRRRSLATSGSVAQLGLIWAISPAKALVQHKSSNVGGFFLDLTGGSLQESGGDDEYWVNVHGALMAVAWGLLLPLGTLLPAHRWLLRDVKVAGKHLWFWLHLGCQWTGVALFIAGFVVAFVKFEEVEGDMSEAHEKIGIAVMAAAGAQVVVAYIRPDPGAKRRGLWNLVHHNLGRITILLAWANVYIGIYLAHTGWGASYTEWVTPIAIVMGLLLVTTLVLRLQTPQAVKSDKVGTLVAHESAAPTGEQDNKIAKEGTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8U3</accession>
    <name>A0ABQ5S8U3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Regulator of chromosome condensation</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009523</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028641">
        <property type="entry name" value="RCC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46207">
        <property type="entry name" value="PROTEIN RCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46207:SF1">
        <property type="entry name" value="PROTEIN RCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="81"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="133"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="190"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="256"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="357"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="418"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="48711" checksum="07EA0E4049F8142D" modified="2025-10-08" version="1">MAAAATEQKAWEGGVLLYCGGTDYAQLGRTGGKTKKEDLDKESQYPNLLRPTRLKTFNDVKVMFIAAGPAACHCFVGDSEGNLWSWGRNEKGQLGHGDFAQRNVPAKVAGLEGKFVIGASGGRNHSACVCQDGSSYAWGINQHGQCGTGTVKPAKPPLKEDIVLTPTRCLIENGTQVACGIDFTVWLCDGKVMTAGNGQYGVIGDGVDHSYNAKDSSIQILYEAVATPRRVVGLDDVKVLKIAAGHNHCLCYDDQGSCYSWGNGGYGRLGHKVQQDEDKPRRIEVFTRRNLVPKDAILAAGGTGSFCTGVAGQVHCWGKLKVNGDNQMYPVPVNDLVGWNVRSFACGPGTFAAAADNSVITWGAATNGELGFGPHKKSSANPAKCEPLDGGYTHQVACGIGFTVFLVDPAHPRLAETTEWESSIPVEEAPSLEAAAAAGAKGGNKRKPAGEAAGGAKAGKKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S928</accession>
    <name>A0ABQ5S928_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_009298</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040221">
        <property type="entry name" value="CDCA7/CDA7L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169:SF15">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169">
        <property type="entry name" value="OS05G0300700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Zinc-finger" evidence="11">
        <location>
            <begin position="185"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="322"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="322"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI65691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="355" mass="38659" checksum="F87677BC6CC893A0" modified="2025-10-08" version="1">MVSTRSRRNGGIEEIQPTTVDARVKPRATAKDAAVRPEDNAAAPVTDLDAERARCMARNAEALQRFGIKAALDALLTQAAAVKGKASATNLEAKGTKRSHAHLGQIPVRRSKRLAGAPAPGMVEFDDNGNLVQQGESCAGLLDLMENGDARIKPGIKVDELTDEQRTAINVQRCSSKSRGGIYHQRGLTCHFCRQKKLCGEDDCPRCSTQNWEADCIGKTFCRACLSANGIFCRACLIVRYGQDLDEVRADPTWLCPHCYVDKHGHYNTHGWLCNSSICLRREKIAPTGIAIHEAIKQGYPSVAHMLQARLLKKAKEAAHTGAGRGTAQTATSTHAENHQQRLTTTAVEVELDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9D3</accession>
    <name>A0ABQ5S9D3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Selenoprotein T</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009773</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019389">
        <property type="entry name" value="Selenoprotein_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02174">
        <property type="entry name" value="CXXU_selWTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13544">
        <property type="entry name" value="SELENOPROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13544:SF0">
        <property type="entry name" value="THIOREDOXIN REDUCTASE-LIKE SELENOPROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="17523" checksum="B6AFF18DB291E1E7" modified="2025-10-08" version="1">MRGAFAQVHQLARTRFPGIDVHGTPYPLAAWKRPIVAAAQAIQMTALAFCMSGDYVFRQLDIQPPAWYTRNVMANRFGSAMGVWFVGNMLITNLQNTGAFEVYFDGKLIFSKLAEGRMPVVQELVGPMDAYFMAAAAGGGRPKAWSTGDKAVVSDNDGGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB21</accession>
    <name>A0ABQ5SB21_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_010833</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000044">
        <property type="protein sequence ID" value="GLI66849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03236">
        <property type="entry name" value="ABC_RNaseL_inhibitor_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034348">
        <property type="entry name" value="RLI_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009945">
        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="57"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="81"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="356"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="621" mass="70236" checksum="80ED980AAD3BEFEA" modified="2025-10-08" version="1">MPPKKGEDRKGGEETLTRIAIVSEDKCKPKKCRQECKKSCPVVKVGKLCIEVLPTTKIALISEELCIGCGICVKKCPFEAIMIINLPKDLEKETTHRYGPNSFKLHRLPMPRPGQVLGLVGTNGIGKSTALKVLAGKLKPNLGRFTNPPDWQEILTYFRGSELQNYFTRVLEDNLKAIIKPQYVDHIPKAVRGNVLTVLEQKDQRDVKAMLLRDLDLEQVTDRNVENLSGGELQRFAIAVVACQQADVYMLDEPSSYLDVRQRLKAAQVIRSLLDEDKSKFVIVVEHDLSVLDYLSDFICCLYGKPGAYGVVTLPFSVREGINIFLAGFVPTENLRFREDSLSFKVTEQMDAVEVVKKFSRYKYPKLKKMQGNFTLEVEAGEFTDSEILVMLGENGTGKTTFIRMLAGALPSDLAEGDDGEVAELPQFNVSYKPQKISPKFEGTVRQLLHKRIRESYIHPQFNTDVMKPLNIEGLMDQEVQNLSGGELQRVAITLALGQPADIYLIDEPSAYLDSEQRIMAAKVIKRFIMHSKKTAFIVEHDFIMATYMADRVIVYEGEPSKHAFARTPQSLQTGMNLFLRNLDITFRRDPTNYRPRINKWGSTKDREQKEAGTFYYIGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB45</accession>
    <name>A0ABQ5SB45_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051357">
        <property type="entry name" value="H3K9_HMTase_SUVAR3-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45660">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45660:SF13">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="537"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="393"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1008" mass="108258" checksum="E57E5B54342B5890" modified="2025-10-08" version="1" fragment="single">VICETYKSCKTGCKTRGRNSIEGHFERLERSKVDGRLACVLIRYLPNQWIGFALSICIVMNKLTDELSGLAIVNEDDEVVIEAAKWMPRVELKCRDKNCLLLGGHRIATNTIMVWCTCTQPDNAHLDGSRLMALDAWVIEHCRGEKLDDYEDEEEEDEDDDAPGRVDALCMEETLVVRAGRNVLPKQVSFKQFLSSTLVAQGGTKALKGTLFWVYWYDQPIPTPNCPEYGGMYRTWAPGSLARQGLGVWYRAKIMRCDTSRGVVQVKYDIDASTDAMYLLVAFAHFGAEAPVRGTKPQVLLDMDLVQTLGLPRTAVASLGPAKGKDHNGGGAATPAATAGASKRVAPAATAAAVVKKPAAATAAAAATRKAPTRLIAAAETKHVAAAKPASATAAAAAKRSHPSVATKSQAVKQPVKSEQDKSHKGLTEKKKSAREAIKTTAAAASPPSLIVKLQQPQTAGTDAASPPSGPKPLDSIPAMAPKDLAMMKAPQTPSKRPASSSPPAPPKSPHPATMGSGAAAGAVAPMDTAAKKFRWAEIQQQPTKKQKPDAPGMLASRRNSPAQPGAGAAAVAGVVAMPEIAPRLSKRPRHEFDSVTLALTDTTRFVPPNGSPPWPQPPLHPLAWAEWEGRFRTSRPDGGFTGWLGAGNEQVPLPVYNTVDQTQPPQFEYISLKDFEGQLSYMLTEVTRGQPAVAEALQGIKQIGNAAKPPWGQACGRDYHDRVRQRDLDEHGDCDGAGRFTPDTNYGPDGRLLVTRPMGIHECSPSCSDPRCHTNMQLSHGVRRPLEVFRSCDGAWSLRCAEPLQEGAFVAPLLGRLTSAYQLMEEAEAAVAAGERWPRAPEVLLLNHFFGMWEAVFTRGLNWCEQHVPLWQLPPNPFSIIIRRTHSSSGGGGSGGGDPAHPPPARHLTCMLALDTRQVGNVARFVRQWTLPRPSAATVPTPPAVAPAAGPALLVQPVLAGGCRSPLLYYMGLYAARRVDAGEELTCDFSAMALGRCTAGQMTVLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDC7</accession>
    <name>A0ABQ5SDC7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012249</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="4">
        <location>
            <begin position="61"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="466" mass="49826" checksum="58B1D32E3D04C34F" modified="2025-10-08" version="1">MRLQQQQPMSQRYRVRTDFRKNVNFKLIVPISRGTASLRVRAFQTSESVGQDKQQPSLHTKVAIIGGGPAGHTAAIYAARAELEPIVFEGFMAAGIAPGGQLTTTTYVENFPGFVEPILGYELTDKFRQHSARYGTQILTETVTSVDLLVHDTLKPPGLYRGLPSRSMEDADLGAPKQLAHAEEVQRPRRPFKLWTPSRCITADTIIIATGASARRLSFPGSGDESEGGFWNRGISACAICDGTSPLIRNKPVAVVGGGDSAMEEALFLTRYASKVYIVHRFDYLEASKAMARRALANPKIEIKWCSQVVEAQGNDQGNLGSITIARSPVDAHKSAPVGLSGTVPPPPSSAFLHERLDVGGLFFAIGHRPATDFLQGQLDLDEYGYIVTVPGSTVTSVPGVFAAGDVMDRSFRQAITAAGRGCMAALEAERFLHALHAAPDAIGAGSEGRQSGHGVAGGMVAAAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDY4</accession>
    <name>A0ABQ5SDY4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012206</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45093:SF2">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45093">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR MSS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="947"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="217"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="969"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="979" mass="101788" checksum="6582C8FA73603EF8" modified="2025-10-08" version="1" fragment="single">FEWSQSLPAHITLSYHGSLCKVLGSYAASPAAIVVVCCCGACEKKLAVPFRSGGSICSLQEWSLHVTLEPLVLQDLMVVQHAIAASPVPFSLWMHAASLMMGGDKLKRKQLCVYWYNSVHQDVREPSLPSAPSTTATVGAWRAATVIDFDARLSRFTVKYHTDHKKQQSYLYLPITLLHFARMPPAPGVAPRNVAEGFQPLPSVILQTPPTPPLAHLQLLQTQQQQQQQQQQQQQQQQQQQQQQQQEQQQQQQQQQQQQQEQEQQRHQQLPLLSTSISPTLPTAAQLHSMPLLPMTALSQPASQLASLPTFPLADAPGWSARGTVAAATTATTAGWVQLNSAPSTATAAVPHGGSPQLQRPVLDFNFRPLAAMTQQPQLSALISSRLGRLGSTGSTGSTGSDAAGNDGVATMHGPITAFQSLCADRAPALAEAMLSATVAAWQQQQQQQLLQQQLQQQKLLYSQAVDTASDVAADTSVGSGFQTEDVVTSASTASQAAATTTNSLVSAGMDLPAAPAASLAAVASGTASAAIDAPACITVPHAASPPQGACIGFLSPSLAPVSSHQHSGAVPSSPFSALLGQPPSHITSAPQLDSAPLLELRPLPPLVQPFGSVATAGAATTTTTISELTTNASCASPFDLDSSICGWRQQSSSAPVATADATTAAVVAPRSTAEVHGAGYPSQLVTTGGAIYSSKSTVLAVASVRALPGQLLERLAAGSTVMPAGNGVVMDLFGKEEALATAVAAAGAAGIAGCADSNAGRCGAPPPSTAMLGTGLEDAAFMEEMVQFFSSECTAAVPTPPSGKHPCQSAPPTVTEFLTTAKDFASPDGLSGGSSASIANFLAGNDSRNGNNNIRNQAPPKMPISIPKCSSASLAAEAAVAMAAAVSASFQSSATDARFNGAGAVSATAAAGTMVGREEDDRDDEAWASVRREVRRLENLCSWANHQQVDSERVMNEEDPNNDSGIHQSKHLLRSQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE87</accession>
    <name>A0ABQ5SE87_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="243" mass="25388" checksum="E2C3A83D3C25FE1F" modified="2025-10-08" version="1" fragment="single">DLTRLFARLQLDDGDEWMGAEHLSSCQPVACASEFGPKWSDDWSDPYELSDRASALEHSLRRRSICSAQFSLDADESHSSCTATSSHVKLGSALRALHVSVSSEHTVEGPLGAPRACAGGVSRRLEQLPWGADFLPPYMSAFGETSDEVSRVCGLPPVPSLITLSGDNESFISTAPGTAGNPLVLGDSFSPARAPAMGVGLVAGGPGADATHIGAVAQCSPLRGVVKVRKTEVSNEEKIGEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEC0</accession>
    <name>A0ABQ5SEC0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Iojap/RsfS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004394">
        <property type="entry name" value="Iojap/RsfS/C7orf30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043">
        <property type="entry name" value="IOJAP SUPERFAMILY ORTHOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043:SF0">
        <property type="entry name" value="MITOCHONDRIAL ASSEMBLY OF RIBOSOMAL LARGE SUBUNIT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02410">
        <property type="entry name" value="RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="746"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="786" mass="84492" checksum="FA83C50C0AD8C123" modified="2025-10-08" version="1">MLWQLFSVWPLGCYRVGFRQGRRDLQLDIVSILSWCKYTTSSSGRRIGSILRDDVSLEAVLGQHHSASQHFEEALRDAVKERLAVHFGRHGRRCLDLSSFQRLPAAELVQACRQARLPIAQEEDPRLLAVALHQWLHAPEVALRPVGSNLHDDGWDRPASLRQCLWIRQGGDFFGDNATGAVGNSVADSKAAVDVVVRSPAGSAWPAVRRGNDVTVFRDGAAVAGHAGTGEAEVALRAVSERQADILFDDELQWRLQQAAAGAAEGVLPSAGESGLTLHSTRVSAAWRTRSAKKVQQQAMGSQPLKESPAPRPSPQQPQMHHSEAQLQERIPGHAISVPEPSFLLTTNLLTTTGSCAPSHQPVSSVRGASSFHIRSGGHDDNSLLNLLLPVKGSPRPSEVVAAVRRSLLRAGYTSKEPTTLRSLLLLSRGELLRLVESFNRGCLAAAGQGRGQLAARLLDMITCTGSSVSSDSNSIGIGTGSSAGSNAAAMHSRQTCKISILPSEVGKEVARAGEPPSALVQAPGGDRRRVVTWLGHGTRMDGSADEHEVWDDERAALGDVRRRQVLETARRRQQLMAAAARPEETATWLLKARAQDVVLISLDFDSEDSAESPSTSTNGSRMSYMIVATALSHRHAYACAEAVRYQIREKLQELASECGPGSGAANGRSDGSHSVAAAAAIQGDVSVSGFNGADWLSLDAGRVQVHVLTPQARRYYRLEDLSAGAGDSLDAARGSGVRIRMDRDAAPTMDRSPRQPRMRLFGPLVDGKRLPDTPETAKVGQRSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SER7</accession>
    <name>A0ABQ5SER7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRP15-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012459">
        <property type="entry name" value="Rrp15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13245">
        <property type="entry name" value="RRP15-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13245:SF14">
        <property type="entry name" value="RRP15-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07890">
        <property type="entry name" value="Rrp15p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="384"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="43633" checksum="26CC8B4D7F4840B8" modified="2025-10-08" version="1">MAKFQKPKVKVPVKQDGSAGEVHDAKGRPVSASQRETYSGSPTEDSCEKQADLVRSAGKGRASSEGEGKEEDVLGAAVSGSEDEAGAEAPLGEDEDESEDAEEGDSDIEEEDLDLLPDEFREEGDDSDDAGDIDIGGGSGSGSDLMADDDSAEDEEEDDDDDDAHVDNDDGDEDGERQEARRRSGSLAGAAPPGTGGDGGGFLEGSKGASFAKAFSKLLKAKSKPSEVGRVPILEKSKTVAKRQAEEDADAEARRDAKRARQELRQRGHVKVPRKGEDPGHDQLEKQLGRLATKGVVMLFNAISAAQKQAGGAGTGPGAAKKPTKISKASFLAQLRSTGNGVEAAAGGGSGGGGVLSAAAAAAKGGSGEAAAAPGWKVLQAGFSGLSGGGKMKDWDRKPTGSDEEDAQGMGVGGAAGGTSSESDDGEGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF78</accession>
    <name>A0ABQ5SF78_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013013</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006746">
        <property type="entry name" value="26S_Psome_Rpn12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="77"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="266" mass="29461" checksum="423C878EDF3CE5F1" modified="2025-10-08" version="1">MALSEATQLHGRFKDAMGKGDISTADNLLGQLKLKLIQLPALPPSFEQTPTAQQELLLGREVLESAVLLSLKQQDEGAIERSFAQLRTYYNDTRTLLPPSSSEVSIQGLNLLRLLVQNRIAEFHTELELIPPQAQQDPAIMQPVQLEQWLMEGAYNKVLAASKQSASEVYNMLMAQLATTVRDEAASCSEKAYERLRVADAQKLLLFDNDAAVRAYAQEHGWTITMDDYIIFQSAATDGTAASKAIPSLQLINNALVYAKELERIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF84</accession>
    <name>A0ABQ5SF84_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012386</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="119" mass="13304" checksum="080BBC1C40E8245A" modified="2025-10-08" version="1">MATQDPKATGMIATRGPVLPHAVNGVRITSTSIRHMCICTIFGMEKQVKDIKETYLQKVASYIEANFVTGDALRRQIRENVATEVAINSQRGMHHVRMSRHTKAGLQRSPLFAKDAKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFY8</accession>
    <name>A0ABQ5SFY8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013326</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12365">
        <property type="entry name" value="RRM_RNPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034201">
        <property type="entry name" value="RNPS1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481:SF0">
        <property type="entry name" value="LD23870P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481">
        <property type="entry name" value="RIBONUCLEIC ACID BINDING PROTEIN S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="68"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="161"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="234"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="356"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="410" mass="43362" checksum="03EA49E5AF735FF0" modified="2025-10-08" version="1" fragment="single">SSSGSSGSHSSGSSSRSSGSQSSGSSQSRSPSPRGRATSRDRREAQANGNRQQSPKPSKDVIVDAGATRLVVGNLTRNVTEDHIREIFSCYGLLKSVELAMDRAVNLPRGFAHVEYENHDDAERALDFMNNGQIDGNVVRVNFVILPKKRSPPPLAPPPGRGLSPPRRGGSPPRRSGASPPRRGASPPRRGFSPPRRGGPSPPRRGPSPPRRGGGSPLRRGGASPPRRVMSPPRKGPSPPRRGLPSPPRRGGASPPRRGGGSPPRRGNSPARRRSPPNRPGAGRSPSPPRRVSPGRRPRSRSPPPRGFQGVRRPASPPQRRGPSPVRRRSPARRSPPRRSPPGGGRRRPQSRSRSRSPALAPRGGRAQSPARRPARGRSSSSDRSTSSSPSRSSSSSSSSGGSSTSSASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG05</accession>
    <name>A0ABQ5SG05_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="302" mass="31708" checksum="52854927E7ACD43B" modified="2025-10-08" version="1">MRSAEAGLGAPASEGRAQTLTARVPSVTDTGTVEGNDIDVTAGGDDDNLAGPSQGKVATTTETPVFAAGASTNPLTAAAQDEEVPEHRSSSVPEEAAPVNSATPDLVLACWQAMERYAPLPQPQVTDECNPSNFQQQQQQPVDEHQLLQELLFGALNGPSDTEAAGACPASGPDESADDLEESFSEEEGGEEEEEAGSEPTAKEEGSALEEPDGVSSQPSTSGGILHPAEQRRGELLRKRRRRLMAEEMRGLLPPHSSPPSADLQTHLDNVAASLIKRHCTVPITDPLVTAYGSVCRDAVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG80</accession>
    <name>A0ABQ5SG80_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IBB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11667">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11667:SF27">
        <property type="entry name" value="ADENYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="418" mass="43410" checksum="50ECEE3A523B24F8" modified="2025-10-08" version="1" fragment="single">MKRRTAPKAQDQYNEEATIGRRRQNVRFESSRQRDVNLRGRRLQVELSGVGASQLSQAALVSLGPDVLAERVKKLVVTLQTAPPIELLGLLQQLTTLLSTGLHEVVEAAVTGQVVSLIAAILQQRDSQLPAGSTRAAACALELVASASVTAALAVRPAVAALTSQLTAAVAELGSAAGGTTTTVAVDRDAALLDAAQMAAPFGVMAGWGYELQDCLTEAGAGPVLLQLLLATIECAADRASPAVDAVAQVAAGDATQLALLQPGPDPPEVHCCSTALWAVGMLIRDRGDAVASLVAQPALLAGLRRVLLAPTPYPELLRGVAWLVAFCSSVDWPAAIKHLVDDGGLLPGLLLSSVRFAHYAATLNGNDPILEEAAQAVQRTLLPLLLAAANIAADPDHTLLVLAELHAPRPLTPELTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG99</accession>
    <name>A0ABQ5SG99_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Squalene synthase</fullName>
            <ecNumber evidence="3 7">2.5.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013518</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 (2E,6E)-farnesyl diphosphate + NADH + H(+) = squalene + 2 diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:32299"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 (2E,6E)-farnesyl diphosphate + NADPH + H(+) = squalene + 2 diphosphate + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:32295"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phytoene/squalene synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.21" evidence="3 7"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006449">
        <property type="entry name" value="Squal_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044844">
        <property type="entry name" value="Trans_IPPS_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01559">
        <property type="entry name" value="squal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626">
        <property type="entry name" value="FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626:SF2">
        <property type="entry name" value="SQUALENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01018">
        <property type="entry name" value="Squalene/Phytoene_Synthase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01044">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="459" mass="52514" checksum="01B60D581F26F271" modified="2025-10-08" version="1">MGKLGEILSHPDELIPLVRMARDARQVTRLPADPDLAFCYGILNDVSRSFAIVIQQLPNPLRNAVCVFYLVLRGLDTVEDDMALEVGFKVDQLKLFHENIYKRGFTMSCGYNHYKRLMSKFNIVVDIFLRLEDVYQQVIADITRRMGYGMAEFIEKEVITVDDYDMYCHYVAGLVGIGLSQLFAACGLEAPSVRAQEDLSNHMGLFLQKTNIIRDYLEDIQEEPAPRMFWPREIWGQYSTSLEYFKDPVNRKQAVQCLNHMITDALRHLPYCLQYLRQLRSVLVFRFCAIPQIMAIGTLALCYNNGKVFEGVVKMRRGQTAKVFQTCNDMGDVYRWFIYFLRILEAKVSSEVAQDDPSLAAMRTEASASLSHCRAALVQWRAAQPGSAVALIRDWALLAVSGSYFYYVWRLGGPGPESLPELLRPYAWVLERHEEVQRAVSVLVLVLVFAVVVSERRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHN6</accession>
    <name>A0ABQ5SHN6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="762"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="419"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="801" mass="82443" checksum="1FEC85F9484D6E72" modified="2025-10-08" version="1" fragment="single">EAEAALDIGGLAALLWASVMMQARPADPRGQWMAELLTAALRKLRNVLTAPTVVLAAEAASGDITAAAAAATPLSESAGASLQDMASLLSALQQMMYDPGPDWINTCLQAAAEMQARTDPSAAVNAAAATGSPQPGDALAAVMFLAAQYDHPQDDETRGSVVGMAQEAQAWMQLQEDVAAAAAVEAATAVGSQAAPPPLFSSRGLTQLLMALVRMGGRPSEEWVAAWAAAAEPLMRCGGGSGGFNVDQMVAVLATAAEADVQLPEQLQRAIVQGLLRPQPAVQTTPNMPAPTAAPAGAATPGISGPGAFLPAGPGAAATDVATGSSGAAVRSLMQFRQLLAILELQTYLLETDWLEHFVALVEDFRLGLSSRDLLAVLRVLGSFRDTLLMDTSDAAVQLASPSRPVPSSSPAQAGHRPATTSATASATTSATSSSRRPGGPLVLRPRESPAAAGSSQALPPFPFPSATSSPQSPGAAPPPKARGLRDISAASSSPPAILDSAWLRSLLEDLKVRGGVRGPNDLMQLAQALSALSAPPSLMSYVEVFAVAITGEDGRRRAPTPRMAVGATPPAVSGLAAGTEPSSLAATAKPSVARIRLRLQQEAREQKAAAAAPAAQVAATATNGAAAGAGSEEEEEETQQWSLAWPAMLGAFAASGHIPERRWWDRFAAATLPALELYEGPRLTSTAMAALGLSSQAAAASAGSDKGGDEEEEDKGTLAAKLLPSLDWHQRALTCVAEAIRSCPTSMDEVREQAVQVLSEQRRQSQRVLGMGEDVENDAVELTDGADSPHEEFTSEELSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SID6</accession>
    <name>A0ABQ5SID6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nascent polypeptide-associated complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014398</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the nascent polypeptide-associated complex (NAC).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the NAC-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22055">
        <property type="entry name" value="NAC_BTF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039370">
        <property type="entry name" value="BTF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10351">
        <property type="entry name" value="TRANSCRIPTION FACTOR BTF3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC-A/B" evidence="4">
        <location>
            <begin position="59"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="195" mass="20336" checksum="AF4CFA1C0A4CDE61" modified="2025-10-08" version="1" fragment="single">PLLSLLTQRSPSGSVLLCWGPLPPTNMDRDKLMKMAGAVRTGGKGSVRRKKKAVHKTTSTDDKRLQNTLKRLGVNTIPGIEEVNIFKDDTVIHFVNPKVQASIAANTYVISGPSQQKKIQELLPGILNQMGPDSLVHLKKMMQQLGAVGGLPGGLGGLGGLGALANLAAPGAAGVAAAAGDDDDVPELVENFDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIQ1</accession>
    <name>A0ABQ5SIQ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF19">
        <property type="entry name" value="KATANIN P60 ATPASE-CONTAINING SUBUNIT A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="509"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="637"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="811" mass="85312" checksum="71E7D00BE65F1B11" modified="2025-10-08" version="1">MTMDAEPDKPALERHREALKSLSRRELQARAKALGVRANGKSVDIIESVAKALSRPGSPGKEANADKQGEKNPENYARLDEACVSSKLPQAAGDELLTSISCSAIHPATSSTPGPVTEGPLKDLAAPALTDALAASLDNLHFMLPMQAAGFRFAQASLRRSVQHLRYSESMRGSVDMSQGKSSSFGCEQDELTTTDTTAEEQPRVNALVNDAALVRDALLLQESTRRRRVLAGNCQDDAIRRSLSRIPTREFTAAPASKFFGNAVTRSADTENSGKVSADAVSPAAVLVSRQSPGPSGGTNHDVETCHPSSPPLALTTQHTSAVPPGAAGAAAATPMVVRHVAGPVRAPGLFVTDRTPLRQQNLMNSQLQALGAATLTPAARGAPPPRTPGTGLRTPARRVAVCTDEAKERAMDEWVAQNLVTAAGSLAAPAGVSWGELVGLRHAKQRLEAICMNNFATPEASSPFSETQVRAASTTAAGVGKSTILSKLIGGPVAHGQTRAARPGNLPNTAVLLCGPSGIGKSHLVRALASELGAAYVHVPSDLSSRVAQAPGGAEIVMRSISRVAMQLPGPVVVHIEDVDRLAGVDVTRQRQDCRRLRTELVVAVDELVLQPLSQLPTPGQSCTMAVAIGGRGGASPASSSGRSPPAAEVRARTGGNSNGASKARFKVLVLTTTSRPMDLDVSLASSLGRTERILETLPGPEDRELYLVARLAAEGAALGVTQVECLVRQTEGLTCAQIAALCDCARTTEAARRATSYSHGPVFVTEEDIMSVLQHMERQCTEEDLAKLEEWHMRPSGVVQYHNGPLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIV6</accession>
    <name>A0ABQ5SIV6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014610</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_625, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45333">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1842"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1885"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1918"/>
            <end position="1939"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1948"/>
            <end position="1970"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1990"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="2029"/>
            <end position="2048"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="2060"/>
            <end position="2086"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="242"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="375"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="417"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="501"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="590"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="847"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="932"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="974"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="1059"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="8">
        <location>
            <begin position="1852"/>
            <end position="2093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1300"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1494"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1539"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1620"/>
            <end position="1676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2355"/>
            <end position="2394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2450"/>
            <end position="2518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2531"/>
            <end position="2639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1307"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1322"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1494"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1513"/>
            <end position="1523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1541"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1620"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1652"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="2498"/>
            <end position="2510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2576"/>
            <end position="2586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2607"/>
            <end position="2622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2625"/>
            <end position="2639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2639" mass="275997" checksum="5F0BBD27C34CC70E" modified="2025-10-08" version="1">MADDDPGPRRPGPVTKRSWTTVLRKVSSTATDVGSADAGDSAKSGVQNPLSRPASNGTSFRKVARTSSNVGLINLPATNSLGAKEGANASGVLSGRSHSVMHGGLLAPGQATGTTADVVTAVKDLQLRKRSIMFQQTASQTGPSLQTRTGCLSTYFFAPNPTPGKGVCFSPDGAYVMRWGYGTDVKLLSADDGVLVVTITGPTPGAVRYATFNSDGTKVAVATEDCKVYVWMIANKYLINMLDVGAEPLAHVVFSRDSQHLLSCDDEGQLELWDITEARMLRRYETGHISKRCGFSSNGESVMSCAGDDCGLVMLKTRTGAVETTQMYEEVAESRNRACYVISPDGTKVLLFIQEQVYGINLVLFHTDTDTHIPLMVCDGAVTHADFSNDSSLFITAGRDDLIHVWECETGMLRTTLQGHDSDVMFCKISDDGEVAVSGDSAGTVLVWSLSDSTCVMRLQAHASPIAYVDVNADGTRALTVDTEGNALLWFLDAARAFEVLRQHSDLVCCVAPTPDGSSLVVGYKDGCLRCWNVCDGPGLAASVSWTHSKQACPVEQLVMRPDGAQVASAGRDGIVVITDIRRGLVVATLNAHSDFVTGALFSERSDLLVTASRVGEVFVWNAKMYSTGPLRQLEVPELTEVASLAISSNQAFLAAATAGGSVHVWSLSSFVLQSDVKVTDLPATQLTFNADASVLAVGSADGSVTLIDPLTGIRAAYYKGNASSINDVTFLMKPEHILLSVCGRQAIKWDCHRSAIQHVTDFIADSSRIFSSESIGLLPNRTCVAHDAVVLYDPINHAAMYDMVPRPPEQHPGNSYLFRHGTALTAGTNGHPMVLFCAPDTTVHMLSDETSGTRCLDFSSDGRLLVSGTLRGELVVWDVVHQQQMHKWVAHKRYAITCVRFTHDATAVYSCAEDCKVIMWDWRRQTKLAVLVGHQAPVQAVEMSVDGNQMASGDRDGFIFIWDTVTHAPKQVIPAHDGSVLCCSLSPCGTMVASTGADEHIVILDVNEGRQLASLDDAMDGKGLTIKFSFDATRLLVGGEKGGVLIWDVSRNCLLYDIPAHDGKVFDCGWSGDSRRLLSVGSDGRARLWDAAAGSELCQANFERVMGAVESGGFVHCDLSATGSRMAGCTAKGQLIDWDVGAELRCVPEGSMLYQRLSALSVGRSREVYVRLLRQFPLLPNTQDSRGWTILMHAVANSNPEITKLIIKSINPGSGKLGLTASAVQNSILSSIRHVPRVQMETGASSGKHNFSGLGGAQAWEGSGKRGSIPGNDMNNTMIKMGSQATGVSSKKLTLLEPLNKGRVDVDEDENLDEDDERESTTSGAAQQRTEPASPTAVAAAVMGAQRPPNSPGGGGGAAGAGLLSRMGSFLRTGAELLSPTRERTSTPNESIPAATMTDVAAASGSPAASAFLDATSPGQERPIRSPKPALAFAPESAISPTRASPLSSPLTNAGGNLPSILRRAGSANFNTPGAGAGAVTEAGGATFTVTLPGTDSGPDQPSITGRRSFGSGSGGTDGSVRGGITYATGRRSLLGSVLEAERERDSGSRSRRRSFMFGKGVQDGGGGSGSHLTATPLGRSIRHMVHRLVRVSSRAKDVEAATLPEDNVDVRHVSDLSRNGWQEDDHDQSRSQSGVAGGGGNGAVGKDANSVGSATPSSSSNRGSNKVWPESQTLDRTEPNVIKIALDAGAADCVQHVLDAVLAQKVTPGSYHAITAAIPDVAQQYPTMCQVFLSGLPLRPLGEMEVPSSIAARGMIVTSAPSYTSYKEMWMHELQLRGGNKGPMALMEACMVPLPFAAASGKTSVLHTLVESSVPVQTYGSDTVKAIVDYKWRNFAQRRIYIKALVYLVYTIIFTIFAVLFADQNIEANMKDLSKRKSGKAMLAFCGVLFLYGTYFLILECMQLASLGFAAYFDSFWNIVDIAAYVTTLVVCPCTVFRKGIERKGFVAALVAAEVIMLWVKVLFFGLAIDGVGTFIFMTAEIFKGLKYFIGLLATLFISFAVAFMVLFRLEPEEVIDPSTQGTMGTLYGSFGNSLLNVYLVMFSIGDPHDAISTEYGPFAIFLFCVYMFALLVVMLNMIITLMADIYQKVKGIQHFVFLKGRAELIIDVESTTGGGLTNAGQPYLHLLMPLRKDLAASGDDNESDKLVTASLPRGTAGTAGLGPLAGALSRAVSLTGGLGRGVGGGGCGGAEGLSGSASDDDDGRSVAGRALSRMPSFKSMRSFAATTAGGGGVGGGGVGGGGVGGGGVGGGGVGQRRATEAFGATGNMTMRRGAGAGASAAMAAPDSERLERLERAVNRLSVSLDAIMSHMRVRRQSNSGVNAMLVAESPSLGTTASNRRGQRATVTGVGNTSMLVRGQGRGDNSFGGPLPGGVLSRNPSRPTSALGPSPSLPPSLVVAHLGTPSVVPSAPVSGIPIAACANGGGPGGAHAHLHATFADQFADVAGRPARTETQERASSAEAHASNSGGPAIVAGGSPGLANVRSGTPDKAPVGPTGGIGTAAGAGGMRTSAPEDGADLQNVVASPVSRKGTAARPSDGPPSPSPPSGSGAGGGGSQVYKRGSQRGGTGSGGTDRSSNSVSSSAGGGGVVTAARLVLDPEEPTAWAGSGPGRPAGSASSNREAQRIRTPGEVDSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIX6</accession>
    <name>A0ABQ5SIX6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Intraflagellar transport protein 80</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014245</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056456">
        <property type="entry name" value="Beta-prop_IFT80_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056157">
        <property type="entry name" value="TPR_IFT80_172_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24098">
        <property type="entry name" value="OUTER SEGMENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24098:SF0">
        <property type="entry name" value="OUTER SEGMENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23335">
        <property type="entry name" value="Beta-prop_IFT80_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23387">
        <property type="entry name" value="TPR_IFT80_172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="100"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="182"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="IFT80 second beta-propeller" evidence="5">
        <location>
            <begin position="302"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="IFT80/172/WDR35 TPR" evidence="6">
        <location>
            <begin position="611"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="765" mass="85768" checksum="126B45269513F99D" modified="2025-10-08" version="1">MRLKVKQGSAQVHSELTGAVGWNVWNELYSCSDDQTIHKWNMLGEPEQKICQLDAYFTDTHWYPLSSKKTQAGGTDVFAVSCTDGSVKILSRTGRVEKSIEAHRGACIALRWSFDGTALATAGEDGTVKIWSRNGMLRSTLAQADSPVYSIVWAYDCDQLCYCTGSNVVIKSLTSNAKQNAWKAHDGVILKVDWSPINHLIVSGGEDCKYKVWDSFGRLLYQSGIFDYPVTSVAWSPSGELFAVGSFNSLQLCDRMGWAYSKVHLKDTGSILSLSWTADSTQLAGAGGSGAVVFGQVVDLTLEDGKMQVTVVDEHQIVVNDILNENADELPEFRDRVIKVSLGYGYLIVATATQCHVYNTNNLNTPHIFDLKDTVTLLLQAERHFLLLDNSTGIQIYTYEGRQICNPRFQGLRTELLNTQMISLSNDTIAVLDQQASGTTVRFFDTAQGRPLGDPWSHTLEVKEIALSQAGTIADRQLIIIDRNRDLYLLPVMKRSIAKLAAMCDSARWHDTTAMLAAMVDQRLCVWYYPSEVYVDKDLLTKTRYTKADSDFGKSAQIQFFAGNRCLVRRSDGVLVSAATSPYPAVLYDMVRRQQWEKATRLCRFIKDPTMWATLAAMAMAAKELNTAEAAFAAIDEVDKTHFVRKVKLIPTEEGRNAELAVYRRRPEEAEAILMQAGLVYRAIKLNIKLFNFERALDLAVQYKQHQDTVLWYRTQFLKAAKQQETIPRFLQMNESVVVDAVAIKKKIAEECIKESQRPGAKRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJQ7</accession>
    <name>A0ABQ5SJQ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_014220</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1020.10">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035898">
        <property type="entry name" value="TAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000197">
        <property type="entry name" value="Znf_TAZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808">
        <property type="entry name" value="CBP/P300-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808:SF1">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02135">
        <property type="entry name" value="zf-TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00551">
        <property type="entry name" value="ZnF_TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57933">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50134">
        <property type="entry name" value="ZF_TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TAZ-type" evidence="13">
        <location>
            <begin position="204"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="298"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="431"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="884"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="347"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="884"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI69713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="924" mass="96692" checksum="5F92FA5FF6521391" modified="2025-10-08" version="1">MQTLLLQVTLFGTGNYQFTSSHFLCNRWMNYAIYVRRALRKFLRESEVLQLDTPEGSVPLVGGLLWFCTARMSSLGSRAEVECSDRFQSIGDIVFTEQTPKIALSSILGNRSRIERTHVRQSIAGLTPKEHQAMNDVCVTTLDPLKTPLKPSRSDDRCQLATADHAEASPCNDDRVLSDKDIIQPAAEARKASMRVPARVKCATDVHRALENRHRWLLFAQHCYTCSRTEEDCDLGKLCTYGKKTLKHVARCKRDDCSYPRCALLKPLLQHSRKCQSSSCAICVPVWNYIQRELQQQEEQQDAQRAQHQRKALRRPSPPPHRQRHHSSAAADDGVSRTRWVPPHPPSSTGAPPGGSAASDPAVEQRRPEDELCGRSSRPEDAQPGSSLAAGALGGLPEWEAAGRLSYTSTPGVAGCGTAGSHLGGALECGKQQGGTSDVKPACADKTRSLQQQQQQPQTQQQVPREQQTPVQAPGAVPDVRIEPLQCAHSQKPSSVHQAQQQILQMPQKQQELAQLQQSHQHQPIFTIAQPMVQHGAAAAITSAVPQRFLGVASGTLPPYTFASYSGGLVTSLVPSPAEITEHRPKGITINAAPQQPIPVISCTAMHPPPPASSSSQQLQVVHSYPPSASLGSFYSHLSQHHPKHPGGPTATVATTTHWLPFPGLTAVPRGASVTPVATSNGINGVGAGNGGLFVANAGAAANTATAAGPSAPAAWPSQGPIASNPVALYGISADGMRTGGLGTVTTQGPVQAVDRFSGLPYTFAGVNGGGGLPAAAGAMAIAAPPLVLWPHQPAGAMAAAGPAVVSGAQGHLTAHLPTTYTVTSTVSARPSSGTAVLEVSGAAATPPGPGTSSTQGSAVAPTHSVSTHRLLYGPAGIVPSAGGASAAASITGSSGAPVGVSASSTGPVADSATGAAHWNYLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJT2</accession>
    <name>A0ABQ5SJT2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014652</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF128">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="358"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="312"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="422" mass="44510" checksum="94C424BE568938A9" modified="2025-10-08" version="1">MMLTEALSTAADALSRQPLSTIIPQHQNIVCLEHSTTVGAALQLLAKRHILSAPLVIIPGLEDLSPDDESSALPQLIGWVTVESILRAFLTHMEDKHGKLPRNMLLLMTQLEKEGPTFAEKPLITVSGSEDRGLLFQVHSDVSLLTALRESFVPADKPAAHRLAVFDASGSITAVISQLDVIRHLREHPELLGDLANATLEQLGWPQAPGVASGRPLVTVEASLPAILAYERMARAGVSGAPVVADSEAAVGSGGGNGSGNMIANLSMSDLRNIQSQHLGILALPVGEFLALLHNTSYLGYSQRTSAQAEHPFFASGPPSVLPPPSPRQRLPSMSGLSPGRSGEAAGEGEEAEHCGAAIGIRMITCTPDTTLLKLLALLSDNKVHRVYVGAPNAAPGPLVKPVGVITPTDILRLLAAHEVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK59</accession>
    <name>A0ABQ5SK59_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="619" mass="64361" checksum="277DC08CF1F74E14" modified="2025-10-08" version="1">MAKAAAINMSDAFSPPPQTHVITPPSTYGDFLPSASNPAGPGPSSAASQRLSGQVRALSASKERLSTGSSYASKRLSGPVASHSPSTTPMTAPSLPSDIGTVIQPNAKLPPLMHGYGAPSPGRMGTGTSTTVSQHGDKQHPHGEKQLMPGEKLLLGEVVQPFIQHPSILPGPTPASYPYQPKRKPPSAFMRNNPMLQQLDNHHRPQQQHAQLRSVSAGSARTNYGLPDNSYGASGGGVEGDYGGGAAGSGDLPNDAQAQQFAAEVMAAAEAAIVQGKLHQPWGDQNGFSSAADAGGFMSTPPWGVSSVSDLEALDSILDQLVVVDARMRKALLKGPLISFETVVPEHVMNSFAAPAQQQQDEEKERTFLTAVKGVVEEGVAGEENSGADHGGAPAMPPPPSPAELEASSLGGAFTAASPSVRQPQRTIEEITEPRDSALFPASIPNPGMHPTFTRAYLASKMASKPQHLPPPPPATAEHLLRSRPPPFIAAPSYDQLCSQIRDVQRQYTCGSSRPLEKGATSAGAGPSGVVTAGSITAAIAAGVPIAPTAHQYISGLDGPTLDEMVAHQELPISLERVRPTFVALKQKARRSASPRRRPMSPGRSAGHTGGDGEAVGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK68</accession>
    <name>A0ABQ5SK68_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="195" mass="20502" checksum="0E3E5CCF0AC54FFF" modified="2025-10-08" version="1">MKAEWTTPCGQEIAKRVSSSEESIVAAFQAFSSFDVQANGCRSPGAAAGGRHKRCRGSMITARLCLLLRAELNRMRLRNQGATATAAADACGERPLSGIGTSGEMEGANTSDRPQSGSWRGVRAGEVVLHMSSGTEAAPTSAEATTVEDEEEEGSTGSGVAMVFASAAARRLLVEAATHITDVQRQKRVERESVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKC3</accession>
    <name>A0ABQ5SKC3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aspartyl/asparaginy/proline hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014656</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartyl/asparaginyl beta-hydroxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007803">
        <property type="entry name" value="Asp/Arg/Pro-Hydrxlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05118">
        <property type="entry name" value="Asp_Arg_Hydrox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aspartyl/asparaginy/proline hydroxylase" evidence="3">
        <location>
            <begin position="457"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="609" mass="67183" checksum="068C991E215002F4" modified="2025-10-08" version="1">MKTVWNVIRPHNWHPKTSQEFLVEFDAETGREVNRVHIESRFTHDAVRRGDRVYVANTEMGEVQELHFPDMKLVRGMRLFTLKQHVNTLAPLDDGTVWAVLHNLGKSDAVKIDPNADPRPRVVAEIKEVGLKAHGLVSWRDQFVMLDSENGALVVVDPATGAVRHIWQAPQTADPAKFLKGLCVVDEVAYFGLNVWGPRSNRDSLVNNAELAAVELNTGALLWQRPVQTRGLLNIVAAPGLGEASTYRAMASTGGVAEASFASTTKGSGTSTDTAATILATTHDDGDTGGGTSSASASSKQQQQQQEEQKAAGSGKDGGKARDVLALGINDAEVIAATQQLIDMGYRPKTSGKWASGQPRLDLAVKDSVHSWEAGISLPLMQVNIDKLKELVLGMPDDMWTPERQRRENAAIDGRKDNMNKFKPGVEALYMVFSDQSTEHVYRFPYYDHFKSAIEPLLEQVLGKEDVSKVVRLQFARMKPGTEIKPHKDMGGYAKAAHRIHLPVVTHPNVSFQVCPEAERQALMGRRMSAASTEDCVPIPMAEGMVFELNNRVMHLVLNRSNVKRVQMVVDVAEEPRVPRRLKPGTVCNYDRAIMVCPPENVLPDYSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKI9</accession>
    <name>A0ABQ5SKI9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014744</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="458"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="545"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="638"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="696"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="799" mass="86910" checksum="2FC8D995658A9EAC" modified="2025-10-08" version="1" fragment="single">MIGFGMSCSDSQPEYLVLQSDYVTNLRSAYELRIQELQRSASKQLAGLQKKHDQALLDFELQLLEAKGESARLRELALSWRIKHVKSLVRLESAEEDAQLAQANVSNLDLALQGLTAQREKLLCCYQASQEQVQSLTALCQTQHRAKVAQQGHLVNTDAEVKQLHAQLLQSNDLAEQLKQRLEGEQRMVEAIRAQAEDLKQQLSMGQAELRAHASAYAALKGDRDSLQAALAEEKRLRAGLDMRISILSSELQACSAELKAGEQRVERATTDRVRQEELMQEFQRRRTADAVLIQELEQGVERMRELLEQQRRDTGKELSVLRAKLEQEQALRESLERQCAEKLKAQDEQRKTRLAEREAAAREQAQQLEASQYERRLLQLKDAHATELETFQARIRELEREITIITSPDKVQPEKVQRTKATIDALIGKLGGGIGEDGDEDSPGPPANFDLGMSQPHFGQTRGKLQMDASDPKRTAPVAPLPEAATTADGPGNALDKEALAGSVAGTAKIAGKRALKSSADEGCPAGGRRACKAAKLMKAVDDVEPPATAPANEDNKEVPRQADGGPAARIGSRRRAAQSGQARTKAMAAVLTNDSESEAIIGTPGVEVKGVRTRRAARLVRTVTTTEADPVEAVVEAPKQARPAAGRSRATKAANGSKPQGQLLTEPDPGMADKQPGAAGCAAELNPPLVRQVVGNSIPRPEPHENSNTGRGAEALPNFSQPGLPTASQVSFFSSFQDLDTSKGRNPLATLNQKVLEHARGRARIVPLTSIQQWKIGISGAHKNAGGQQLITAPTMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKS0</accession>
    <name>A0ABQ5SKS0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C3HC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015523</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012935">
        <property type="entry name" value="NuBaID_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15835">
        <property type="entry name" value="NUCLEAR-INTERACTING PARTNER OF ALK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15835:SF6">
        <property type="entry name" value="ZINC FINGER C3HC-TYPE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07967">
        <property type="entry name" value="zf-C3HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C3HC-type" evidence="4">
        <location>
            <begin position="46"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="353"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="451"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="607"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="361"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="466"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="615"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="672" mass="69617" checksum="563F6682AB55C53C" modified="2025-10-08" version="1">MASVYERITNALSSLGKRRERDNEDGGRDNRLTPGEKGRTPKRYRPWEQSDLHRRLETFKPLTWFGKPETVGPVPCALRGWINNSKDSLSCEHCNAKLIYPPNISYQQQQAAADLFSPNLATKHNFTCPWRSTACNTSLLAYIPPNNSEELCQMFQTLQGKLLKVDVLPEVDLIAIQALRNAAVPYGSYDELVMGPGGGALTPVHKSTPAVRIKDVTDEPYGVQDSSAPLLSDIGLPLNGAAVDDTPTFLLQPSKMTPLQKARLLALMGWDLDVLQPESASGSAALLYTQSAGYGLGHLGVKPKTALKGGSGEGAAAADDKRYPMSSVVLKCPHCNCRTGLWNFAGLRPVPTGRLTPSQHPAGASALLSPTSASTPTAPGGAAGTTSSAAGVDPLSVTIAGGQYGLHGTARIGSTASRPFGATSSAPFRFGSASSTEPVFGIAALEVKAQKSKGDGSSSTPTPAAGGFGSPLGGGGGAASTPEPRRTGQKRKAGEPFTPEPMAVDVTAAAQTPTPGPSGGVASNLTDSKRLRLGSYTSGGGTSLTGGRAQTPAGDVRQLPELDPVGQHRNWCPWIYTGTREEPHISGWQHMLRALSIQQQQQAAAAAAAAAAASPPAPAPAPADGPPAASGVIPMDTTPAFQAAVPTATGGGASAVDARRLKDSALAAIRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM65</accession>
    <name>A0ABQ5SM65_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053099">
        <property type="entry name" value="WAS/WASL-interacting_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48226">
        <property type="entry name" value="OS06G0326200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48226:SF1">
        <property type="entry name" value="WAS_WASL-INTERACTING PROTEIN FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="499"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="553"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="704"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="499"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="581"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="717"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="730"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="815" mass="84973" checksum="669FD6DDB6EFBC0E" modified="2025-10-08" version="1" fragment="single">QTQQVMNPGLSLSAGDNTPPPADELPPAAVSLTAAPSPHVAAAATAAVPQVQQALGPTHQQPLAAPAPPPPQHSDVFVTAVGNPSAPLPHFWGGPISLPPVGPHQPPYQPLPPPPPQQQRQPLVEDRLTMAQVPMGGWHGLSYGHVMMTQTQQQQQQQEPMYGRLGDPVGRICNIESPDAAAHISADHQQPAAVAGEGWLAAGVFGRGPVQQIASEPSPPRRPPLQQQLQPQQAQLLQRSLSSSMSEPYLLQVPFRMHLNQPSPRPPLRRQQEEEQEHTQQEGWRRIQSPGNTSNGGGGGSAGSGTGGGGNDNGFGGGGSEFSGGVIALFRQPAPQAAFAVGADPPTTSHHLAPVKAPASSRLAPALAPELGLGAAAYAPTKSSFPVAGPMAQADRVAAVGAPAVSERDMAAATSAAERYRLIMASPEGQMAIRWALERKAHERALKAATEAGAHLPPNARERWLFQQHVYDVTLRQTRLQQLRLQKYMEEGIQMQRLRQQELQQQAQAATDTGGTPGRQQVPTPAPPTEHYALHQSAQLPSSSGCRTTHLFQHMSLPPLPPPQPSKFAATFPETARSERSGSSANGSAAKAARDGVRSACAADAAMAVNGCSAQHQQHSFLPMAPSYGREVAVAATAAAAPMAAETVGTAVAVTTSSAGDGTTAFEHMGGCGCGGCGSGVPSAFETTVGTRRGEQVRILGTMLHDEREQRVQPSAGYGGDDGSGGGADGDFYAGNAGQARRSGSGNDNSGGAGSNGSDLKLKGVAEVKSGNAVSYNERATAAAALYGPNPYGTLYHQQTALIAAEDEQEQRYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMI8</accession>
    <name>A0ABQ5SMI8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015581</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF39">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="7">
        <location>
            <begin position="48"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="294"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="297"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="599" mass="62167" checksum="EB876A9622AB0844" modified="2025-10-08" version="1">MSTYISHDVIRKLCTEIYAETGKRAHVVDATWNMPAWNNLLNNSTILKPGRPSCYFQPRGSDDVVAACRVAARHGLKISARGSGHHYGGASLVEGSLLLDLSVMRSAEVDPATRTARVGPAVTAQQLRSALAPHGLHFPLPHLSDVGLSGYLLGGGNGWGVRHWGAAADNVEEIEVVLLAGSGPAGGDEGGKGGGAAGDAVRDPESSCRLVRVRSETNAELFWGLRGGGFFLGVVTEFVLNLHPVASRLPLIKATYPLSLLSQVSEFFWKLHTSNSSRVEASLAIVGAASATRATDGSDASGNSGSDSGCSTLPADGGGSGGGGHGSRTNVAPGNGHASPTDGRARAVVLLSCHAFADPESDEVEVPYGKLRAFLPEARLSFQEGTVPYEEALSVLDASWNWPGLCMYGHGIFVPVSSLHINDGVDGGDDDDDGAPTTDDCGAGGVVATLAAAAASLTSDRSILLLCPSAPSRPESAATQAVAPAACGASGGCSAEARGVVPGALGFRDTLYLGIYAMWSRSRTGPDSDGDGDGDGDAAHVAWVRLCSASLEPYTLGLYVNEVMHDEPGQVRRSYEELSYMRLQALKKRVDPSGLLRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNJ4</accession>
    <name>A0ABQ5SNJ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sirohydrochlorin cobaltochelatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016177</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000102">
        <property type="protein sequence ID" value="GLI71105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03416">
        <property type="entry name" value="CbiX_SirB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050963">
        <property type="entry name" value="Sirohydro_Cobaltochel/CbiX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542:SF3">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="209"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="27485" checksum="581DFC5A9D926CEB" modified="2025-10-08" version="1">MRIPKPTSAMLSLGPKHTLSTTFRTSQIGYKFALVVNSYQRACEVGWANVNGGPSTSYSVPSRCWRRQQLSAKPRRTAVLTSLKSDESFHAHSLTTNNDDKVGVVIVDHGSRKKASNDMLVEFGELYGRTTGQEIVEIAHMEIARPTIEEAISRCAARGARTVVIAPYFLSRGRHIQEDIPTLVRQAQERYPGLSCVIAEPIGPYAVAGRRHRRAHGATDQQPGGSSTEIAGSGGGGGGGGGAGKDGSGSEGPDPGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP54</accession>
    <name>A0ABQ5SP54_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="2">
        <location>
            <begin position="133"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="610" mass="67631" checksum="C082771B91C51696" modified="2025-10-08" version="1">MEDCDEEEENPLIDASDSDSDEDESDLEFEDNANENEEIVGASPGGDGDGSLSEDPNIGSGSNSGTAGDSPEASPELARAARVNWGIPRERLNLAVVARQPEVPRTYDEAMASSASQMWKQAMDEKIASLRANQVWTLEELPKGVRALPVKWVFTIKRDVNGEIVRYKARLVAKGFAQVEGRDFDEVFAPVSKHTTLRVLMALVAAADLELHQMDMKTAFLNGILEEEVYVLQPRGYGEGNQQKVCRLQKSLYGLKQAPRAWYVRLREELEQMGFQVSQADPGLFLKDVQGERVYVLVYVDDLLVAAKSMATLNAVKNRLMSAFDIRDLGEARVFLGYEIERNRTEHTLKLSLTRFITELVAKYGLEEANPRKVPLSKALRAVGERLDVQQFPYGELVGSLLYLSVGTRPDISYSVGALSRYMSKPTVEHWQAAKGVLRYLIGTNVGISYSAKSSVGTLHGYCDSDYAGCVDTRRSTTGYVFLLTGGAVGWASRLQATVATSTAEAEYMASGAATKEALWFRHLARDLKLRVPCVPIQCDNQAALKMSSNLITTPRSKHIDVLYHFVREHAARGEVKFLYCKSEDMVADVFTKPLPFPNFSKFVRAMGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPI4</accession>
    <name>A0ABQ5SPI4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">WD repeat-containing protein 75 second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_016768</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000114">
        <property type="protein sequence ID" value="GLI71536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057644">
        <property type="entry name" value="Beta-prop_WDR75_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053826">
        <property type="entry name" value="WDR75"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44215">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44215:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23869">
        <property type="entry name" value="Beta-prop_WDR75_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23769">
        <property type="entry name" value="Beta-prop_WDR75_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="43"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="233"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="WD repeat-containing protein 75 second beta-propeller" evidence="10">
        <location>
            <begin position="420"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="670"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI71536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="868" mass="90228" checksum="B2776A6904897DF9" modified="2025-10-08" version="1">MVGGGSLSRRPGTLSGDGKLLLCPCGQLVSIYSAVTGQRVGLLVGHTEEVTCVILDPENDNQVYTSSLDATVRLWDYRAGTELRRLEVREPVKHMVVQKELGVAYFSVQTREGSGRVMLYNVRTGKFSGNALKTRSAGPLALSRMGTLAATFDRHSLFVWRTGVDVYQPLNLRHTKPYTCLAISADNSLIAAGDVSGRILIWRSFEHSVPSALRQGEHAGKAHPTKEPPLTTVHWHAHPVGALVFSEDGTYLLSGGEEGVLVLWHLETNRRSYLPRLGGPILGLHLSPVDPAKYLARMANNVVCVINTATMKVETMVLGIKLAPHVPMLPPAGVNGGAVAVLPAGSAAALGGGSGGGGVLVLSCDNAELQFYDIARDQHLKLMQVVPRNAISSTESTMSPAAAGSGGGGGGPAPPAPFVVLLALSPDGSVLATVDVRPDAGPYGSTEACLKFWDSAALLKSGSGSNPKGGAPFMLNTRVDEPHRDVVTSLVYHPCRHMVVTTGGSGLDSEFKVWIQEGQRPEAATGVHPITAAITTAATRWRCRSIGGYKSLPMTAAAFSADGSVLAVAAAARVTLWDAESNALVAVLPAAAAGPAAVVGPTAATPLTQLLFIPGTPFLVSSSATCLAVYNLLTATLHWCLPLHAMSISADAAYGLLAVAIPVIAEEARQQQPQQQQHQGVAPAAAQQQAPKIKAAAQQRPVPRSCHVVVFDPRDSTPRYHCHVPGAARVHLTHLPAPAAAAGGSSGSGEGAEGCSPLLILRDSRQYSLAAVPGSKALLNPAKSQGGALESDPALSAFEAAFGKPMVPDKASGAAAPMDTDVPAGAATVPAVGGRPRWADLFDAPSHALPPPTALADSFLKLLTSTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPK4</accession>
    <name>A0ABQ5SPK4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_016590</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="1367"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="938"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1173"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="952"/>
            <end position="968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1586" mass="168118" checksum="8FB768E09F536037" modified="2025-10-08" version="1">MEKWLSCFPCRTGKEQKQRCYTCLPEGTLLRSGSEKCSTFPTSAQRSSAINIKHTSQLELALQYGRSADAVLVLACPRALLDVNTDDTLCELEPVYCNLSAFKMLGLPIFVLPDRDSYEFATNVLTALRNMEPALVQDAGKHLRETITSDTSINFTLDVNSRTSQNPQARSPPALPNRLNEHAQDTADVSSLLQSAPVTMAASIPRPWRHVVLDTVMYESSGMSTAHANDVAQPATTMNGSTLLQRPVPQRRRSLLSRVATIQARMLTKTFYPKDSKCQAPASTTRHGRMVVPAVVLLFQLHLQQPPQPTSYPPSPDTEGLVPKTTGSIWSLSPVSALPTDSSSHGVTDSALLPAPQANVMTFPTPACDSSPYPPSDSGLPDAAACERGSSRAGNDAIMHRVLQLGHHHSRNSRTLMLQSSLNRRGGSTQPATARRAERYKLAASSSAGGKKRKAATSASTTAAARSGEAGFRAALAAAAAASEITSATAVTAEEEGSLLETPLLRHCLSSPSKNIADYFRAHEGMVLSGLTPVVTAFGGPGCMEVLYQNVTSILYFGLRAQPKTNNGCPVAKALKDGSSSGSSTLIELFSIEPIKLERLLNDVLSEGRVWRGIVQVPATCHPRLTRLQRAQQTMINPTINLISSDAPSAAAARCTAAHYSSPAVSRCVHTATKPPIPTAALAPINPPKSPPTQQQLSALPHVSIEVGMAKSLPHVQGSDAAIAQTISLKATVTTMMADYPTSFSTSPMLPVWPPVAALMAAPVAPTQASIVADFPGSIDNIVLSTLDGITLATAMASADVDPQKKVLGNSEMDPLATSTWGLMIDTPEQHLTTTTSHEMYHRAATEHVSGFVSAMGVPYKSKKANPGAATTTIAAATTASPQSMIKASSYRTIREKIFGSDASIMSSLLSPPGAPLQEGCASIRPIVGISVGKRDDAAATANERQPPPVSLVSQYRHQPSSPRVDDSTTAANTAIYGRAGGGGCCLDNNMARASFTVMGRVMSMVEHDPHLRASCSVGGPRKSHRRTDLGFADFGGRTVPRVLTFASTLRSTSSAAQAAFQAAMASSLTVSSRWSVVEPPPSASRPFLKPESWTNGLKEFVKEPEDEGLGVEPSISCHISNGSPEDAGELHAAVPTAMAILNPDKLHSAGASGSCDGGVDRNYTATVRGVHNNEHQTDGNNEEAAAVGGGPVSEERMTDMMRECSRTGDGDVDDDSAPVCGDTITAKPRSIPTAASAPPPALQVTTSAGTCWHEICASRTTDPLTGQYALIITQTDVTGKVEAERHLAFVTEAEHRLLEQIFPRHVLAYMTEEGGPWAVPSAPTLTTATTTNTNINDNPVMPDTTIGWRPIVRDINKLATSHDQVTLLFADIQGFTPMCKVLEPRVIMAFLNDLFTRFDSRLDEFGVYKVETIGDCYFVAGGLIYQDEYGMPAVRALGEPADPQHAERVLSFAKAMLEAASGMSLPTTGGPLKMRIGLHSGPVVSGVVGQRMPRFCLFGDTVNTASRMETTGVPGCIHVSEATRSLLGKEDWVPTGGIEVKGKGLMNTYLWTPPDPKPPNTPLMGASMATAAVAAVTASAVSGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ45</accession>
    <name>A0ABQ5SQ45_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Carbonic anhydrase</fullName>
            <ecNumber evidence="4 9">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_017061</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="4 9"/>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03124">
        <property type="entry name" value="alpha_CA_prokaryotic_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041891">
        <property type="entry name" value="Alpha_CA_prokaryot-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF265">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="11">
        <location>
            <begin position="68"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI71746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="390" mass="43422" checksum="632D5FA2DB672314" modified="2025-10-08" version="1" fragment="single">RLYILYRDGARNSYLSRVHRRTTAAAMTLTQRLTLLFWGLLHIFGLVSACIYKYGTSPDSLAPPHGGTHWDHGLNGDDWIGVDAVGNPWTCSSGIRQSPINLPATQSLELNGDTLSRNLRTSWNYPTIHSNGSNVEVINNGHAIQIEWLSPYAADVQIVVPARQSNSTSVTAVLGMQQGEQSTTVRATPTQFHFHTISEHLIMGKHYPLELHIVHKITDLPACTSGCYSVTSVLFELTDVGDNSLLDSIWAAMPMREGQINFMPAGTEIRLGELLPHINVRDYMAYEGSLTTPPCTEGLLWHVMVHPQKISRAQWTKYKTAVSFKDCAFKNATSTAGHRHHRHHHHRRMLLADEGPVGNPQDYTCTVAAYGFNYRSSQQAYGRAVKLARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ53</accession>
    <name>A0ABQ5SQ53_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Starch synthase, chloroplastic/amyloplastic</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_016919</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18189"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Plastid</location>
            <location evidence="7">Chloroplast</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Plastid</location>
            <location evidence="7">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03791">
        <property type="entry name" value="GT5_Glycogen_synthase_DULL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02095">
        <property type="entry name" value="glgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46083">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46083:SF1">
        <property type="entry name" value="GLYCOGEN SYNTHASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Starch synthase catalytic" evidence="11">
        <location>
            <begin position="155"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="10">
        <location>
            <begin position="465"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI71645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="659" mass="72805" checksum="5B790D8BE5BA3E50" modified="2025-10-08" version="1">MASLKQAWDSRALVRGAHPRPRLVKTRILGPMMPRTVCQVAKIEETVSLEEELMRLRRENELLRKQLALYQQSSPITPDAAATSASRPAVGPEAVVEVLPRPLPTTVEEVGVDGGILWPTPGERFWERAPRSAPAPLERGTDQQRPQERDLNTLHVVHITAEMAPIAKVGGLGDVVTGLAKAALARGHFVTVMLPFYESLPVDQIDDLKHEMDVEVPKGYRWDGEMRVGPLRTSVFWGRVQGCPVYLLRPAEDSNSNIFRGGKIYGGSYNEMEAYLYFCRACLEYLHVSNQHPHILQLHDWHAAAAALLFWEVYNPGAVNSRTRVMLTIHNMENTGECRQDEFFFTGMAGEPFASIDKALDERTIGHNPERLNLMKGGIVYSNAITTVSPTYANEVLNGGAAGWLRSTFARPELRSKFHGILNGIDIEEWDPATDPLLPANYDAERAGGKALCKKFLQQGLGLAVDEHKPLVAVVSRLVPQKGIHLIKAALFRTVERGGQFVLLGSGHSDPVFRSLADGQFKDHPDCRLKIMYSERLAHQIYAAADVVVVPSMFEPCGLTQMIALRYGAVPVVRRTGGLADTVFDVDGSGAAPTGSRNGYTFDGADDGSLYGALDRALQLYEQQPSRWSSLSRENMRLDVSWAKSAKSYVEVYRSIAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQK2</accession>
    <name>A0ABQ5SQK2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="235" mass="24834" checksum="D2CE6DF31FE68D77" modified="2025-10-08" version="1">MASSSDEPRGPLDTFAEDLGRAVLDAAASGNLSAPALLAAATETGFRPDQLATLIAQTTEIRTQLGKYLLSLLNLLKSTVAINTNMDNTAGPRVLPLPQARGHDDIPEDPLGEAVALDQELGATRPTPTAATRRSNRQRQARGGASSGVRGSGGAGPSSDPAKSRKRGGAPSSAAAQRSDPNAKRTRTDDSFMPKWNCNRSEFERRKAANVCLRCTESHPTKDCPLLPPFVPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM25</accession>
    <name>A0ABQ5RM25_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="201" mass="22028" checksum="6069ED41F3EC242D" modified="2025-10-08" version="1" fragment="single">MASWRTPELPGGVSSRNRYQPLDVELQHQNQQEQRLQRQLQQQSQQQEELLARRRQGQAPLQQGQDPQQQRPRETNGRQRLPPDPASQPALQLEQQPVLKSVPGQVSPSNSSSNTGAAAVPVPTRDRPRLSEPPGSAIARGIASGNQLAEPPGGPGDSWPGASQRQMPWGEQSRSATREPRSPPDSNPLPLSSQPTQDHPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RML0</accession>
    <name>A0ABQ5RML0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DUF4203 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_000561</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Nucleoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019358">
        <property type="entry name" value="NEMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13598">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13598:SF1">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047519255" description="DUF4203 domain-containing protein" evidence="9">
        <location>
            <begin position="25"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI58815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="42728" checksum="666A9498DB5B527C" modified="2025-10-08" version="1">MAIRYHQLTYWTVFVFTFLCLANAHTCEEVVSASLKAHFGIVLKCTEQEASVLKIFDIWRPTLVNVTFGSLPGVQLTVYSASTLEELIDPSLKKRTLRWCKVFTRGCLMELPVPTSPSTIYVGAVKPLFNFAQPAYRVETRKLSIPMIGAVLAGCLLILYGAQMSKSMVIRVTGGGLAVGLIMGAIVLCWIRKMLGLRRTSLVGAGAGIFAWMAGLWMPSWNHLLHNTYLLGSLFGLWFFGAVTVYVWGAGMDNPRLDTALVLVFRLLGLLLLYMGTWTNEPLAFAVQVLAWVYCFGPTVWLQRLWGMLSCCIGRNLEDDDSMLLEDRRRIHPLIVNGFIWNPLRVAIPVDGPEFRELLKRGYEPNVYSGRMEKLVEDYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMZ1</accession>
    <name>A0ABQ5RMZ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Oligosaccharyltransferase complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_000305</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OSTC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021149">
        <property type="entry name" value="OligosaccharylTrfase_OST3/OST6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042416">
        <property type="entry name" value="OSTC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13160">
        <property type="entry name" value="OLIGOSACCHARYLTRANSFERASE COMPLEX SUBUNIT OSTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13160:SF4">
        <property type="entry name" value="OLIGOSACCHARYLTRANSFERASE COMPLEX SUBUNIT OSTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04756">
        <property type="entry name" value="OST3_OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI58558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="14713" checksum="114FD96907F81CA9" modified="2025-10-08" version="1">MPRLSSLEELRPSPRLICVLVLVSYFFVTAGIAYDIINEPPAVGGQTDPVTGAVKPMTFMPYRLNGQFILEGLSGGFFYTLGGVGIILLDLSRDKNQSVLFRNVYLALGLAITILSYLVCMVFIRIKMPGYMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN93</accession>
    <name>A0ABQ5RN93_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000223</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF56">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="225"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="326"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="921"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="471"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1045"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="346"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI58492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1129" mass="123622" checksum="2FD2980912BC6B5D" modified="2025-10-08" version="1">MRSSSAIIDPETGESVSCIGQGLLSISQVPELHRLTGLQMLCLHGNNITRVEGLQGLTNLVDLNLSSNAVGSLDSGSLRGLTRLTSLNLASNRLQAVQGLDGLIHLEQLNLSYNYITTIAGLTALQGPQYKLRQLNLKHNQLHNLQAFAVLVGCMSLRCLQVAGNPVCQLPNYMQALATVLAHVTQLDTISSVEALAAPYDVQAAQQYAAMQLQSFQPLPVVQAASAPSSYPSPAPRPVATGVPQPIIQPPNRQNRSQPETVGICKSRPQQQRIGQHQPPRPQHAGLHLQGQGQGASPASQQSKDHAMAEDHNGKTSVTVAVSKPAHGNWQARSRGVEDGDPDTVLDARDELHHHGERDESGTAEDLLPTPDQGQPSPLVLQQPLQPQQRLIKVLLADAAAQTSEYTPLVRRLQVEAVEMRNQLSKLTDELERRNTVEESLRVAMQAAITEAQEEAHRRIEDGFREASFAVSKALQELESARHAANESQQRIAAHVRAEEEHRMRCSGLEKDVARLRDDLQRLNKQLLDQQQAAAAVLDGERRRAAAAEVLAREQLATAQTALGELPVIRAAAAAAEQKVATLEAALQQSSTVTMSMTAKVAETMAEANSQMEVLKARLATAEQQLVEHQRKEVDARAEINTLTAALQATRSQHDKELETAARQHEEALRAVVEKERAAAEERGKGAAALQAQQERAGLQEQIAFLKVQLQFALKESDKEQQAMQQLLRTAQAEAGELRVALQSAAAKQREGEALVADLTSVVQQQKAAIQALQREKEALAARMKACSPDAFDALTTENLNLKRAASERDMYREQLEDARRRWQEAERRVAELQVHSTRTAEELGARVRSAEALLASAREDIARAEANAEKLRQEVAAQQDNVKIKVAMLDSANDTIASLKAEFADLQMEADEARRAAEEAEAALAREQQQHEHDDEESDGEKTQLRQEAQAAESAAAAARVELVEAQSRVKELEGQLRAKMDQIAEKDRMLTYVQEEVDRVQVLFEKRAQLLRDERDAARSDAATVAAARAVAESRLADAGSRIDRLIVELDHSRAQLEDAAMQLEEQRRAVATANEVAAARGAEAARLGARVAEVEHEMRQLLEAVERQKASSAHKMRQLASLLQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNK8</accession>
    <name>A0ABQ5RNK8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000922</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="14288" checksum="9B6E4DA9425E93CF" modified="2025-10-08" version="1">MMVLATNAARIHQKEEIQAARRRQSPQPSKARNSSRNATNSSKGAAKKVTNDSDVRPSTKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRRKKVKSAWEDVRCTRARRQRGSSGSKKKQVTKCPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPB0</accession>
    <name>A0ABQ5RPB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001214</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="1036"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="536"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="676"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="955"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="536"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="555"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="690"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="719"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="746"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="760"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="794"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1015"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1099" mass="114253" checksum="88ABF510FAB1208F" modified="2025-10-08" version="1">MDATVRPATSPESGSIREFAVEHPFIVPGSDAGPSRESTSQLPSGEQPDSVLNSTLPRLASVHLSHNGSRNFNDNADRNGLGDRQLVVTEREADRASRSSGSFYGNTLHIDNVVDQDMDVLTPSRLRLDSRVVMAHATLQWLTNAVWAVLIWQKLDGGLRNCSWWAVFSPTIINHVLHIPMQLLVLLSADYMVYKQIGPPPPPNAASGMVLQYAILHHVRVRSHKVDWAINALESTAMFVVKLQFCDALQRGTLAGYPLRLLFTPIWCCWFLSFVLLYFKDRTERMIGSSRDLFFIFLLLVAFKVDGLSSSSWRVVFLVPWMWFAGLLLVAAMVLLLLLFAKVWARPRELLLPIGFLFLLLSSAPQFVSYSALVRRLDGDTSTSVARIMWPNALSWFLMWLSAWVIWAALRQKEALRDAQLARGAVWTAQRPVNELTEDEIFLRAKSRMVGKPKPGRLQRVGENLYRRIASLDIALAEQAAQAAVQALVVSGGSGPASQTASITVPMAGAAATAAGASAMSCPGGIELPSFAPSGSAGTSASAAVSDNASSTTGGETGPPGVGPGGSGSGPRASSLGLFRNNKVLPAPLVRTVSGQATAAGGGMAATGSRCGLEFSAAMRPATGEAPIDPGPLGPSRPVVDITTSLVRSSRNSLLARAGEEGALPTVVETLGFPAVELERPEEAPDVESGGRVSRNQNRSSRPSNGALQPLPTRGELQSGAQNPSQALIDSPFSPCPSQMNPESGSGSGSGSGSGSGSGTSATFSTNVASATADPGASLHAVPMGQRRDQPTRLPSLLPAPANSTAAASSTTAALSSPTAVSVSSSDRCTVANESPRDASAAAATASGLTIAAAAAVPVAPPATSLNLQELQPGRAEDAAANARIPDILAGKGQQDGEATAPARVSILASRWNTSNVGDGSSPAASFTRRSPAVLPLAEIAPEGPQEGPLGIIDGVGAAGAGTSETGPPFPVGREDVATDSPATADDHMPQTASSSSGASGMPGDGGRDGVATPAAADDGSVAGSDGTGPDPENACIICFDGTATCVLLECGHGGFCRRCAFRQFVRPPNKCPICRAPIDQVVEIEPAVAVGQVAKVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPG2</accession>
    <name>A0ABQ5RPG2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="99" mass="10606" checksum="31290EBCCCC09C09" modified="2025-10-08" version="1">MKAPHLASSRCVLEDPPLPRSVLHRQLLLESAAFATALAAVAKAAAKGSVEAVAVKFEVVEYHVEVVLWAALYSVRRLANSSPFPSVDVLAGLSRGRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPP3</accession>
    <name>A0ABQ5RPP3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavanone 4-reductase</fullName>
            <ecNumber evidence="5">1.1.1.219</ecNumber>
            <ecNumber evidence="4">1.1.1.234</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_001425</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2S)-flavan-4-ol + NADP(+) = (2S)-flavanone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11228"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15605"/>
            <dbReference type="ChEBI" id="CHEBI:15606"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.234"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a (2R,3S,4S)-leucoanthocyanidin + NADP(+) = a (2R,3R)-dihydroflavonol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:138176"/>
            <dbReference type="ChEBI" id="CHEBI:138188"/>
            <dbReference type="EC" id="1.1.1.219"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.219" evidence="5"/>
    <dbReference type="EC" id="1.1.1.234" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0284">Flavonoid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="10">
        <location>
            <begin position="390"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="283"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI59532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="730" mass="75353" checksum="A8A2372844E3BFFF" modified="2025-10-08" version="1" fragment="single">ACSGAVRERGPQKSIVVERSFPPITRFGGVKEALVPMVGALWERMVEDAGLNRRAPAKLLLTWRQGYGAPKTKSSDLSVQALQALKAAAGGAPAAAAPREVGTSASRPGAVGRDGCCGTSDAIALSGPGALAAAAPAPHTANLAPGNNLVSGSSAAGGRWMEPGAVSSAAYQGCRALLEGTAAIFKLGVGNQRSGMQITRLAVAVSYGAVDPTEAVVGQRSISAFTEQLQPQHHHLDKRQRGAAAVPLPNPAAANITPASSVRPAAAAATMLVAQIATSPTVAVANPPTASPASPSLSPARTSGEAQPGPLPQETGLGGGGGGGAGPADGAWDGPVVWAEEMGTSDGYDETNDHVLDFPFAKISKATKHCFRSSTGNSVRQIVKMEGRTVLLTGVTGYVGSELARQLLEARAHLRCPVRCQLNSPRLSSLKRAFDTLPGTITFVDADIRSEDAIAQAAAGCEYVFHVASPFLISAEDPARDIVAPAVEGTASVLRGAARHKGNPLKRIVVTSSVCAIHDCNAKQEPRAGPGKPYTEEDWNGVSTVEAEPYWVSKVEAERLAWRLADELGLELVTVLPNFVMGPVVAAEAANGISVSFFRGFVEARNGADPRPPPSGSWVFTDVRDVAAAHVLAALTPAAAGRRYIVSQPRTLSAKSVTDILKANVPQLGLLPDGDNGSEEERIDASRVVRELGLHYTPLENTLTDMAESLIKLGLAVPAAATSAAVAAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPT3</accession>
    <name>A0ABQ5RPT3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="181" mass="18914" checksum="53BB31CCE74F130D" modified="2025-10-08" version="1">MLVVGAEPGKTTDFGEVGMGDWMRVPQNTGEGRDCQGKRHPFANQGWLQQVQPFMVGMAFAAGLVLVLCIQLSTVGVEFAKVAFPDDPSRGVPGAAAVFGRDAAAVFGRDAAYKAADTSVILMDKASNTVVTVMDKANKASIQIIRTIVAGILVAKFIDVLWKGGPRPPPSDDANPGAGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQL5</accession>
    <name>A0ABQ5RQL5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA polymerase kappa</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001862</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03586">
        <property type="entry name" value="PolY_Pol_IV_kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050116">
        <property type="entry name" value="DNA_polymerase-Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022880">
        <property type="entry name" value="DNApol_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076:SF33">
        <property type="entry name" value="DNA POLYMERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076">
        <property type="entry name" value="DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UmuC" evidence="3">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="541" mass="56958" checksum="241D8677E3667097" modified="2025-10-08" version="1" fragment="single">MDCFYAAVHELECPELKRLAVAVGGIGMISTANYVARKYGVRSAMPGFIAKRLCPQLVFVPLDFGRYTAAAERVRAVFRQLDPDFESGGLDEAYLDVTDYCQRVGCSGTEAAEELRRRVSAFCGGLTCSVGVAPNKMLAKICSDINKPNGQYVLPPDRAAILSFLDTLPVRKIPCIGKVTEQLLRGTLGVSTCGQLLGPERGRLPLLFSESAAEFFLEAALGMAATRHAPKVDTSQEPGRKGISCERTFRNMSQPGEQEAMARQLVESLAADMASEHLEGRNLTLKLKLSSFEVRTRAATLPRYIRKASDMLPTVLRLLRAEGPLELRLMGVRMAALRKVNSAARSPLARLLGRRQQHQPRNTAAAAAAVPMTSAASAGGPTEASAAAVVVIASGSRPHQSPRHVASAPPSFEAPGAAGAHLHGSRALDAASAAAATAAAGNTGDGFRQPIAASVTRTTDPGMSPPRTCWTSAGADADADGGGGCLPTVPPHYDDEYGDFIAAASRWPPAPGGAVPRTTPSVLQVDSVGFRDQAFPTAAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQX5</accession>
    <name>A0ABQ5RQX5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="878" mass="97467" checksum="A131778BF7D91F9A" modified="2025-10-08" version="1">MGAMEAMGAGPQLCSWTRILLSNTRARAMVNGFKSRLVKMEAGVRQGCPLAPLLYLFVAQALFSWLKSQEIGLRLLPSDEDRVTACQFADDAEVALEGEESVPAFLEAMDTFARASGQRLNLDKMEILPIGAVDDHHQGSTINGLKVVTTATALNLPMDNQLQPPQPDWPEMLALAYTRMQRLAHYPLSAFGRAAAVSNYCLQTVTWHMEHGGPPPDTTLTYIERLAARLVDRKQGPEDQGQRFTGVPSRLLVGHPSTGGFGLLPLRQHIRARQAMWAVRFIRGALMARRERERVMLLDTAMNTVAEEEEEPVTSGNVRSLHPWIRTLATYLEITHPAFKPWALTTAQATGPWLGGETLPEEISRIVGALGHLPPLEDVSNPPLAPGDWCWAAALWGNPLLPNNSDNGGSINGNNHRPGLETRHPLMASCRKLLTVGDLVRVYDMLEHDQIPLPQYMTWLDWTQQFLDPSASPTQLRDKESTRMALSALCNDISPAWIAAARKVVNRLQPQQPDRVEDRMQQQMPAPPDRTTVMAMIVRRLGWRLPGDSGIVPLYNLTVSQATQLQGVSTAEERLELLSAFVQEAQGMPTDGPAPPCPVTNLQRFQATLDRLWTAVRWENKNKETLWRLVVDGIPIPGNTHLSRVPIKPCGCGSYGADTDQPCSPRLHHFWECPIAKAVREQIDRHLPLPTSATIEGGLTRQQLWLVQAPPGCEQAPWDVIALAALSSMEYGRHCLRAATREHRPQWPSTHRRRQQQQTHTAEQGPRQRAITDFFNRSGGFPQEEEEDANTHPIISTTQPAMDTPTQTATSPLDRAQARTVADFWARLHSFAALGIPHYGWDQVGPQHPILAIVGTQMRCADPVDITNHGAARTEQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR23</accession>
    <name>A0ABQ5RR23_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001350</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="4"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="40"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="77"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="145" mass="16490" checksum="C3FAD8F6A340FD78" modified="2025-10-08" version="1">MGEERYVEYKEAFNLFDSDGDGYITTKELGTVLRALGKSPTEAEIDAIVREVDPENRGVVDYKEFEKIMRKDIREYNNEQDLKAAWKVFDKAGQGFVSGAELKHVLTCVGEKLSPTEIEELLKEADPEGTGKVMYDDFIRVMLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRZ4</accession>
    <name>A0ABQ5RRZ4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="338" mass="32871" checksum="4B9AD3439C270C1D" modified="2025-10-08" version="1" fragment="single">EGPARSRQGACASPSTGSDSAPHGDEERLGLRPQQKPKPPPRCQEEVAGVAAGASRRELSQGLRTASDNSCGPSLAPRAAEQPSEAAATTGIPTAHPHLGLVASDKPSALRSVQRPHAGVVPSPREVAVAAAAAATAPGQDIGKKPAAVAAGGGGGGGGGGGGIGDAAEGHVRTTSDSVAAAESAAEGGGGGGGGGRPAAAAAASPGHPDDCITSPRVLVSHQVHPRTRQLQLATSLELGGRADEEAGTCIVKSVPAAATTAAAAAAAAVVGQWHCEYAEATCDLESRGLTPGVSTSVVDDTTATAGGGGLHSPEEDGEAPLTQASTIPADEHQQAPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS43</accession>
    <name>A0ABQ5RS43_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_001979</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF52">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE TM7SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01018">
        <property type="entry name" value="STEROL_REDUCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="365"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI59931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="431" mass="49018" checksum="7F14A104974A2002" modified="2025-10-08" version="1">MMNKAAKNPQKEHHFEFFGPHGPALLAFVLPAVCYALILGCNKNSCLQLWPDFKLPKINNNIQYYSQDALITYLAWFFGLALLHVVLPGQRADGVLLPDGKRLIYKLNAFRLFVLVYGAALYFGFYTRQLDLGWLYDQFAPLLTAAIIFSTALSLYLYNSSFWKGLLLSSHGNTGYPAYDFWMGRELNPRLLGGRFDLKEFCELYPGMIGWALINLGMAHKQLAVHGSISNSMLLVNAFQLYYIVDALWCESAILTTMDITTDGFGFMLAFGDLAWVPFTFTSAARYLVDHPQNLSTAGVVLVLAVKALGYYIFRGANSQKDMFRSNPNDPRVAHLRTLKTERGTSLIVSGWWGTARHINYFGDWIMGVAWCMPAGLAGLSAVVPYFYCIYFASLLIHRERRDEYACRLKYGKDWDKYCALVRYRIVPYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS67</accession>
    <name>A0ABQ5RS67_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002123</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="3">
        <location>
            <begin position="12"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="373" mass="39787" checksum="E39CA00EA2FD055C" modified="2025-10-08" version="1">METSSKFNLRNPGVKRIIQEMKEIQEDTSGDFLAEALETDIFEWHFVIRGPRDTEFEGGIYHGRILLPAEYPFKPPSFILLTPNGRFETNMKICLTISNHHPEAWQPSWGVRTALLALIAFMPTPGNGALGALEFPKEDRRAMAARSRLEGPRFGNPDRQAVIDNMHRRMLAISVAAGTRPQSSPDRMIQPPSQEVSTGAVSERQGSSEGEGPLSAAAESGDEVEASSAEPAAASRSAGIPLSIAAAAENIDVGKGNRDASGIAEASWPAVTAIATSGSQLHKRHCPAPQPVEQQSQAASSPTAAAASRRSVAAAVVTDPVHAAIAAPDMMDYALAGAMVVLGLAILFIIARKALSAVGYDLSELLQMFQMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSZ0</accession>
    <name>A0ABQ5RSZ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_002285</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="2332"/>
            <end position="2474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="433"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="623"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="905"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1115"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1232"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1324"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1634"/>
            <end position="1663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1719"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1929"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2165"/>
            <end position="2199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2281"/>
            <end position="2314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2541"/>
            <end position="2569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2598"/>
            <end position="2624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="497"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="917"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1344"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1365"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1645"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1726"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1931"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="2281"/>
            <end position="2291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="2292"/>
            <end position="2307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="2543"/>
            <end position="2569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2900" mass="295539" checksum="1ECD94BBDC499E56" modified="2025-10-08" version="1">MLTNRRLLQQQWGGSHVPIMVASVDQDATVRANGAEVVLAWCNDAMLRLADRKPHDGQDILRIILDSNPRLRSHVRDLLEQMAKGAEVVAHVMECSPTSREKSGSLCNEALYVRILPFYLERSSPGELSSTDSVRCGWNTILPTCFRRSARSILKYEHSSVSMVANGLSQQPALLLQCQPRSLASSIQPKSLRPHGPVVSMGQALFRAHSGNLRTTNPPLAAIVTANTGSKPPPPQQQPPHDARLSFSPFLGGGSTYCSTHSAMGAAVNTVQSVGGPSTRATDCGLTRQFTFPSTIGRQLPHLRNSTAGRLLTSFGATNMSFRGVGGDDVDVDGRSLRERRNSLALAAVPIAVTIFALPSGAVLHQNAASLEYYGDCTSQGLLPTTQPTVTTTYGTAATNAAAGICTNNAANTEGPTPFRSLLETICSDGRELDTNVGGGGDEAGKAAGPPSAAWTAGTRQSATLRVSRGMGLGSETHDLDPKALVPELDQDPDASSDLQKMKTSSSVHLPLPAQKPDGAGACDWMNVRDFSSGAGRTASGGGGDDAAAAAAAARTAKKAVAAARTNVSIDCGASMVVMEVDVLRQLFSYEYAKLERMLASLTNEGDVWQGIIRVPETLNDSALHEGRDGNAEGPRSREHGFRDDGLRQHESAATVGSVAGVNCNRDRDRGIEGDDGGDGGDGGILGSMGSDFHEAMRAVTEGLRQRMQIEGLRQHNGAGAANLRTTEAADGRGGDDDGCSGGVERSMTLIGKETQTSLFTIAAGRDENQDKAKMESAAAMVMAATKTGAAGSTSLNRRAPRRSASAFLLGGLANRLLSSRNGFGRKVAAGAASGVNLALPGPLAGSASVSSALDVDCVDANRFNSINHSNSNYHSHFDEADNDDCGKHGAATVLGEALRANYPSAKGSSFKDIRSSKTASESSSQIPASPGSVGTSFSPFARWGSSILRSRLRGGSSNGGGAATAAAARAGHRGVPAAAAGRGTCGSPIGSDTSLHTPRQTPCGAGCRNALEMSSTPVPESFVSSNGYFGCVNSVGSVAAATAATAAAAAPSLIIADTLRRDDLSVPDVSGSGGGHAAAITGPGLAVSACLALPPRRPAPNRCISIDNPTLSFPAVPEESASEGQLSRKHTQAESDAAPGLGVRSSIAHSGTGTVAVADNRGIGLVAAAAKAARVAKGRVHRVPELLGRFLSYTVDAANRSRMAGVRAPVSPTQLWGSPGAAGGTTVEVVEEEEDDDHLRGSAPGTQQRLPRGVPWRTNQHSTKGYVDDDDDVNEHVVQRSAQSCAVASASSHQLSQAVKEMKREGPGIPEAANLGSVFHGTAAAAADPKRGSGAVEDGPFVRPRSTSRSSLTSHLSLTPGTVRNGGEGGLNSGAGPRSRATFNPVSTMPAISTAAPLAVPQVMASSPPCRGHVRRRQSFSVRRSLQTSNSLATSTWQVLMPGVDLDATSATSAGPWPCPTTTSQRSVVLDPCGLANIPSSPNTTTATNTTTAAATAERPFGTLEMEVANTPMAPLPTTNTVWGLGMKDSQQQQQQQQEMDVQHQWGGVSSIAMCAAASTATTPSVPIVAAAAMAAEAASPILADELHPDSDSQGLNPAGAAPSLGGFCGRHTRLPETAQSLLLLSVRRSSQENLQSGRNLTAGGNGDGGGGGGGEAPGAAGSGTIKTVAVVDHMGGNGGSGAPVPGVSFPVGNTAAFAKTKEGIPVAPKHHIDGSVEGVGMAAPSPPHSPPQPPAEASGPVEQQDLRLLALLTGIGDEIDTDTVAANSAATTERNLAAPSQQLKQPDNCHTAAATTAPTATTAIMVPTTPTMPRQHSAREVADGALGQERITCGDVASLGYVSLSTPMSISGVGVMAAGSGLGSATSTAAAAAAGPSSAWPRPQSSRVLAFASSLRTAPHALLPPGAIPTAVAAIPSSAAVPTPLLPQLASTPSTPHSIVSSGTSGCLTPTGRSRTHTGGQPASAVSGQRSSLRLRAPIVPAHAFSGSSLYGSAGVGTCGGDGVPASGGGVGAMFKASAFSYGLPYGNSGAAAEDAAIEASCTPRNRPSPLLPGRPSMLLRIGSSALSKASLLTSGAADILAPDSEPSNTIATSARVPGGGRSILQQIPEAAMTAAAASADIRSAYTGRTEARAMDMAGNGACNATSAGGGKEGTDGHTSSMVAAAAAAASAGTTQRRQLASPPPHQSVADSPLQPQGPVRECWHEVCATRVLDPMTGSLAVVLVQQDVTDKVVAERHVHQVAEVEHRLLEQIFPRHVLQYITEESVAPSGMQSVTGNILSPSGLTTAESSPDLSFLPSQSPSPSNMTSEHWRPQIRDCNRLATWHSKVTVLFADIQGFTPMCKVLPARVVMAFLNTLFTRFDAMLDYYRVYKVETIGDCYMVAGGLIREDEDGMAAVQGGGHVDPDQAANVFAFAKAMLRAAACVRLPTSGAPVRVRVGIHSGPVVSGVVGTRMPRFCLFGDTVNTASRMESTGVPGSIHVSDDTYGMLQNEPGWQPTGGIEVKGKGLMRTHLWTPPVQSYLEASVYAAPPLSVNQDSLTSRASAPASAQASAPSPPGSGTAASTTGIGSGCVGAKARAPLSSAPMLFTESSAISKRHDVSASGPLRRPQAHGPDEGVRADQQNGEATAGLGIPDPEHLLEQMEALAKSIAELPVASLITATADCNGGGEGDDCSGTIGVGQGVQEVGDSVATAKDDDGSNPCITASGRLFISDILNTASVGKKAPADAGKITTTTTTTTTTTTTTAAAAAGPAAAGPAAAGPAAAVAAAAGPAAANDSAAVGTGSFPCSMSTKLVLDGLGLGRIISDSSTNSERLLSAWAPVMLEAMAAAGGSPFVPRVIDGAPSMNRGAVGTSSSGLGLGGGLAPSSHSRSRSGSFLIAECHASLAAWHRQASDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT75</accession>
    <name>A0ABQ5RT75_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="323" mass="33838" checksum="BB7474B1372D8884" modified="2025-10-08" version="1">MDTHTPVGSLDIWLLDCPTSTPVPTASSIETDALKECGKGASAPVAIPRQHNTHAARTLDSPNPETRSSCMAPWETKPSWLGEEDVREGAKLSNDLVWEGTVRCCRPLDVQSPIGQLVSVPSALRDSQYKQRGNGVTSTAASLPLFTTIAIEVSPMSLASLCRTLSSPAQQGKTLAFAETDSVTCVRGLDRSPTRQCSVLPTHAGVSMSACGPIPVLGGPNPSTTSSVTRVIDQSTRAVCEGTCPADSLVAFRAESTPCVMNNRAAAATAVCELAEPPAAASARANFPHHGGLSTKRGLYEGTVRGASMRNTWQYISCCTAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTB6</accession>
    <name>A0ABQ5RTB6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002797</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="1">
        <location>
            <begin position="2"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="1">
        <location>
            <begin position="131"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="282" mass="30747" checksum="72654FE849BA9277" modified="2025-10-08" version="1" fragment="single">MWTQTESHVLLACYLPNGRHDREVQVEMRGGVLRLGVADGLPVVHRALSGALNPAAPIEVHRTSDGLFVLVVLTKVVQPDSGVGRPLKADEPRVNSTAWPWRGGGCGGDGGTWWRQIFTGDSDGARSLPPPYSVTQSPDEVVLDFALPWWIQKEDVTVEVGLRKLVVMVRGVGLHIERHYWCNSSEPDARQPVVPEFSSWCLWNEGDGRVSKPVAIPPANARVVKSSVSMRVATSNHDRSVVAGTVSRTMAVAATDCSPPCRRHGGRCLSIVLVRPEVTEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU57</accession>
    <name>A0ABQ5RU57_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002833</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="698" mass="74342" checksum="1EECAC446C892777" modified="2025-10-08" version="1">MIRSASFPCGLPPSTITCKTPGKLPWVSQRVGRTEPDQVGPGPSSKSGQIFLNRQFSRRCIRHGTYVAALRKANPPYTHHAAIQSPATAPAVGFRSTASPSSHGSSRISAAATTPTSSRSSTVPADTGASAESVRLSLWLSLLQASAEQFAAAIVSAAAAATGAAVRLQRLSQPLPLNFPAGPPGDQTLALLSDPLSFLSSTTKQYGQLVGFMLGGERVVLVTGREAARVVLLEQAGSVYVKEGTAFFPGSSLAGNGLLVSDGAVWQRQRRLSNPAFRRTAVEAYSGAMVAATRNMLDSQWDSGGYRDVYMDFNELTLRVTLEALFGFRAGEQQSSSISPSSPAAHIAPSQSPSTSSEEAQQIVAAVEKAFMFFTQRAATGFVIPEWLPTWDNLEFAAAVQQLDRVVYGMIARRREELSAVHGSVEAAVVAATDSVVAAAAPNFPSDLLTSLLLSRDEDGTGMSDQALRDELMTLLVAGQETSAILLGWAAALLAAHPEVQERAAAEVAEVCGRDAPPTATSVRHMPYLESIVLETLRLYSPAYMVGRCARVDAALGPYSLPSGTTVLVSPYIMHRDAAVWDNPDAFLPERWRELQGREGYHGYMSLMSNLGPNGAYLPFGGGPRNCIGTGFAMMEAMLVLGAVLQRYRLLLTLPAPQSLSGPARAAETNSDLPASFPKPKPLLTLRPEAVVLWVTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU63</accession>
    <name>A0ABQ5RU63_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="99" mass="10919" checksum="EDD4C28ABCF9EF02" modified="2025-10-08" version="1">MAGIRKKVSKLTNAALALLPAGQAGLAVKVALGLALVFWWTSGPGTEEECEFKAQQDADRRSQYTRHYAFKGRGRKEFLRQDMKTEVNMLVFTKSGAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUD3</accession>
    <name>A0ABQ5RUD3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_002804</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the intermembrane transfer of phosphatidylglycerol and phosphatidylinositol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF0">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL_PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045242922" description="MD-2-related lipid-recognition domain-containing protein" evidence="7">
        <location>
            <begin position="31"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="8">
        <location>
            <begin position="224"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="353" mass="37225" checksum="209DE64A192C9CC4" modified="2025-10-08" version="1">MTVKFFHAFHYRLVCLIAVAAASVIGPAAAANPSADKCAQCISDSSGPSRPVEEASRFRFISLPHKPFKTSSMTPSRLNFLNRPSEPRPQLQGNLRGIADQSNATLLEKTTVNSRASSKAIRTRTGSPQGAISNATDANTAAIAGEAAARDKLAAAATARQILGRRAGTPPSVLYRATLVPSMAVMALGNENELQGSRAKPMYGTDDTDVGDVEDAAAVLSMSWEVCGGGPPGAPMLSPDSVTLAPDPPHHGCTLAVYVSGTNPISTSGGRLRVAVLYMGFKVYTHESALCDAMTCPLEEGAAFELQVSQKLPVLVPPGAYQMEITGEDQGCVCFMCVRISFQMALPAIRVWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUQ4</accession>
    <name>A0ABQ5RUQ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003457</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="39"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="149"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="357" mass="36905" checksum="34B2C367D7FF1964" modified="2025-10-08" version="1" fragment="single">LPILPKGNSVNIVASSSTVSNLGESNSSSGLPNRSSTARVTLLTLVRGAFGSVATIAFILSLQLLSLKDAVTLFFTSPIIALLLDWLLLGRPPGYGGAAATAFTLAGALMVTQPSFLLGDHGRSHRGAEGTDSTSGQSGDGNENGLSSLGVALALVAAAANASGFLTISLLRGLQHPLVLTWWYNLVLAVMTASPLVLQWPQAPIMPSRREVPLLLAVGSTQLAAQLCLNRGFQLESAGRGAAINVLQVLFSFILDVAVLGDKPSLLSVGGSSLVATGVLFVALSSDQRIGPGDGHQCSGVTYDTNRKPSASRTQHRVLVDPGGDEAATRVTLARDPQVAEDSLTEPLLDETAPRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUX5</accession>
    <name>A0ABQ5RUX5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003788</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the senescence regulator S40 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007608">
        <property type="entry name" value="Senescence_reg_S40"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04520">
        <property type="entry name" value="Senescence_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="219" mass="23718" checksum="76F28288EFAD0F72" modified="2025-10-08" version="1">MEPIHEDFQEADFYDLPEVADDSDIEELESEGSLEADEGEERQDDLGVSSAKSSLTITALDTGAEPPRQAADSTFDQLSGFRPIAASMPATVGHIRMGSSVPVSIPHMNRWKKPEPPIDSPSTQFAPATFVPPHQLSQVNEFMFSFTGESPSVAMKRDRLRVRNAILKSTGFLEPCVEQVNNIAMQERNRLAVMAGGLSQALQQQGTSLGSIAAAVKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV79</accession>
    <name>A0ABQ5RV79_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WIBG Mago-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003919</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039333">
        <property type="entry name" value="PYM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015362">
        <property type="entry name" value="WIBG_mago-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036348">
        <property type="entry name" value="WIBG_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959:SF0">
        <property type="entry name" value="PARTNER OF Y14 AND MAGO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959">
        <property type="entry name" value="PYM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09282">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01273">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101931">
        <property type="entry name" value="Pym (Within the bgcn gene intron protein, WIBG), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WIBG Mago-binding" evidence="2">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="193" mass="21068" checksum="74910AF8C9488141" modified="2025-10-08" version="1">MNRKLTEATVLETGEKVIPGSLRPDGTVRKERRIRAGFVPQDEQPVYMSRGALAKQNVPTCPGMDETEVMALKQQATKTKKSSSSVSKPPNPKSVPNTLASPQADSKGAAVSKAVAADPPAQSELPSENEDPKKTLEKRILNLNKKIRQCNTLAEKKQAGVALDKEQEEKLAKSTGWQEEVRQLEEQLAKLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWZ5</accession>
    <name>A0ABQ5RWZ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein ENHANCED DISEASE RESISTANCE 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004488</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009769">
        <property type="entry name" value="EDR2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31558">
        <property type="entry name" value="CW14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31558:SF3">
        <property type="entry name" value="CW14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07059">
        <property type="entry name" value="EDR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein ENHANCED DISEASE RESISTANCE 2 C-terminal" evidence="2">
        <location>
            <begin position="241"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="535" mass="57972" checksum="23F6CDF6EE2D1F3C" modified="2025-10-08" version="1">MAPLCCCFGKRSANRRSMRTQGRQDAGKGKHDDLQSPLSCESFRSEYYDAQENFSVTEGLSPPCSTSVTPRPSVSYEKHHSQGITADTATVLDAGLRPCDGECGATAVAAVSIPVDGECFQDGQLAGTSETSEELHQGGGGAISKLASCLSILDAIRMPGSAGTPTAAGAASTPSPFGSSATQLLSGGPVDGATSGRPTSRRPGRKLEPGSTLLRRDMSTSVLDVQEAWDECDDDNTPSSSGRPFMVRSVDYMRTKVKVPSHGALYRLLAADVISSDTKLTHVARVVNIQHMLRPAVDPHLPPLLIITIMLPMYPATLFGGNDGPSQSLVYYFALPPDFNPVAYTNQQALALLRRLMTNGREADGSPTRDRFKLIPRVVNVDEWALKGPLSPAEHKLLATYNDKPILTRPQHFFFNGPGYLEVDIDIHSYQFLARKAFGAYFGRLKEVIFENAFVIQGNNPEELPEQVLAAVRMYRIDLMRNRPLREFIIPAAAAASAFPNQPGLPARWRSPMQHVPSPPPPIPEAADEPKFTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX37</accession>
    <name>A0ABQ5RX37_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF123">
        <property type="entry name" value="RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE_OXYGENASE SMALL SUBUNIT N-METHYLTRANSFERASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="66"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="384" mass="43629" checksum="626EC1910385C384" modified="2025-10-08" version="1" fragment="single">YITFRTCSVSGWLDIRGKNEKMCATLLGFLFAQALFLIAFTPTSNAYTTTEEHSMLDWIKREGGEFKVTISRNSAGVRGVFTTENVRKGDLLVLIPDHLVLSFRNVPAAEGAPLLLKELYTPCSRLGPYLRILPNESQVLTGYNFPDMYIKYLADDHLESQVQRSFKQHCRSTFEGQNDDKMMMTIPEAIGQVNISLPYYEYVVSMLSSRTFSLRKEALSMVPLLDLMNHDIRDINQLDSSVNARGVRLIAGKDLLKGEEVTITYGDMRSDELLLYYGFLDTVTSPPRLLAVDHRNFKPLEFGELVEEPLTGPEEVIHAEIVRLQRVLAKFENRLKGLGPIPDTIPNLARLLQELHDQRREAIRYELSRLQGLLGQQRDGQAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXI0</accession>
    <name>A0ABQ5RXI0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047361024" description="Secreted protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="16460" checksum="AF788CD00CEE5120" modified="2025-10-08" version="1">MMRGGPPSGLHVCTTLLLAVLLSCVEKLMEHCNPNVQLVFLTPDVLCSPLAGPHSILGLWDLQVLHLLRLLPDFELWIKVLVALWPVKWDRGNVVDLIMDLDHTVALPPAQHKVGGEGGPMSPLATTAGAQGSGSTSVNSWTRVACTIGAAAAAGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXM5</accession>
    <name>A0ABQ5RXM5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">VaNZ11_004989</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16527">
        <property type="entry name" value="RING-HC_PEX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350:SF0">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="560"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="145"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="483"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="GLI62370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="614" mass="64577" checksum="95212C007AE97A38" modified="2025-10-08" version="1">MTTSAHKTVVKLLAASQPDIVRANQKDETYADQYVEATREVIRRLAGPFRALQYARESKLLALLLYHGLTTGLGQSTLGEEYCAMRQVTGEGHVPGPRRRLLLALLLSLGPYVADRLAGQLDNAVEEAVAEVADEPAADTEIVAAPESGVAAGRASGPCLSTKQEDNERLGRSGDPGRFIGGADSDLDSEQRQASGLAAGVGPGSAAAGTAAGTAGTKRLGDALVAPGASGQLAGGSRGWLRLLVRAGAARWLRIWRALALTWPRLRPLLSYAGRLHLALFYGYGAYYTLAHRLTGVRYSLSMRPLQGRPSYGILGALLGLQLVVTAAVQARSTLRDVRRQSAAATTTAKRRTQRRLGMLASDELGTGGEEQLYAAEDEPAVFLDEELPYDDEGRSMQEQPPDEMESPAVRAAAAAAAVTAGEVECKAGGDEYLPVSNGHNGGGGGAVAEGWEEVERVEVDASGAGWEADVFGFKATPPAVLPRLLGAPRPPRPQASPTGPLRGGDIEKATPANAATGEGGWGDINDGRRRHRRGSSSAVAAAAAGSSSVLPLLTSIRQCPLCLSPKSHPTSTPCGHTFCWSCITTWCVEKPECPLCRSAVKLPQLVALYHTNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY59</accession>
    <name>A0ABQ5RY59_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005218</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="37"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="171" mass="17565" checksum="EB6AF6796647BBBA" modified="2025-10-08" version="1" fragment="single">MGLGLVAGGPRADATHIGAVAQCSPLRGVVKVRKTEVSNEEKIGEGAFGQVSRADVFPYGDVAIKWIKPEHIAKHSDAFLVEADILANLNHPNIVRMLGVVTSPPAGDNTGDCNGGAATGVSDPVVGIVTEFVGNGTLGQVVRSPSGRLSTRLCARIALQVNEVASSSTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYA4</accession>
    <name>A0ABQ5RYA4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Initiation-specific alpha-1,6-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF1">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="720" mass="75502" checksum="AE487D13455F8D05" modified="2025-10-08" version="1" fragment="single">IEDAVPAGPIAAVRDSDGGGEEPRGASTDNDIATGTTAATRTQLPETKADGSMAATTTVTTATATASPLYPSVVVDGAADVVGNLAGSDREVVSPSAPGGGEAAGDLSVEAGLRQVVRLEKLRVEELDAAAGEAATAAAEMAAAAAAVPNVKLVTADGGGGGAGDEGDDGGGDGNGGRGEAGSSAAVAAAQPMDDTAVGERRGMSAPREGHVAAALMHDHHRDHNSGHDHYPPQHLQQGQRHEQRDVGGEDSVHKEDHQQLQRRKRKQRQQQHQQPAEGQRGDLYDTRSKKISKESQVDHAALLTVITLPASANRATGPPGAAPGFGARDRVTDDESTAAGGGSTSASTAADNRGFGGGGGGGGGGTAEAEDADMYGNIVTGGGSEAMRQAREALARYRGGGIPRIIHQMYGLFDTTPSPGRRDRLIGPSLNPDPNPDQATVTAAPRRKVLQQQQTQQQQQQQWPQGHKQRVRSLQGWSGGPEVSMQSWRRLNPGYTHILWGPGDVDELVEVMFPAFAPAFRSFRRMPVLRADLARYLVVFAFGGVYADTDTLCLRAVDGWADGLAPNVSAIVGVEADAGAIAHWERFWGRQLQFCQWTFAAEPAHPLLAHVVFRISQLLEQRPADQLVLDDVIHVTGPSVMSDAVYDYLKSKGRDWHEFEGLTHGTLVGDLLVLPITAFSPGVGHMGAGPVGHPEARVQHLFGGSWRTQGFATHGSGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYP6</accession>
    <name>A0ABQ5RYP6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005487</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="183" mass="19136" checksum="F10C61255B382FB8" modified="2025-10-08" version="1" fragment="single">GRGGGGGVSVNDGTSSQADDDDTASKGPGSSAFSDSSSTADAEAAATADPRRARLLRRITKLVAGTGLAEVLWRLKSRVLLLLAAMLVTHITCYIILFRLMKGQFSYVSGVHQLALVADRTQVAVARSAVIEFCSRPGVTPEGVCEMPLDAVVNDVRHALDDLESYHQAIYLGTSSGSGSPIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ16</accession>
    <name>A0ABQ5RZ16_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13361">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13361:SF1">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="646"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="771"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="984"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1070"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1196"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1326"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1435"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1902"/>
            <end position="1922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1959"/>
            <end position="1980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2065"/>
            <end position="2119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2134"/>
            <end position="2156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2952"/>
            <end position="2976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3147"/>
            <end position="3205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3248"/>
            <end position="3306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="646"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="691"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="771"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="794"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="816"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1036"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1071"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1206"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1336"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1960"/>
            <end position="1979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2097"/>
            <end position="2107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2134"/>
            <end position="2146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2967"/>
            <end position="2976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="3259"/>
            <end position="3269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="3278"/>
            <end position="3298"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="3306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3306" mass="335141" checksum="F7DDC424E673D749" modified="2025-10-08" version="1" fragment="single">MDEFYCRACYNEEDLIACDACGACYHRRCVEAHCCGPPVRQPGSTAPKSNSQRTPYQLPPQFPGHGIDGHRSSAAAALVPNGARPPETVLSSNSDFTRPCLTIASEGFEEAALTSEEECPAHAADWFCPACRCVRCGRACCPIAVERGGNRLAAIQNACEGALGLHRHHGNPAVAANAASLCPPVAATGQHRGDAAQLAMWRVMTTRVIAQKVVPGAGADGRGAAIAAAVAVPPLFLEKCRALAASARSPVELEYRLDTEVALNPNRTPALNWPLQALLEALLPTEPWKGRRVALQLLAAALFKTMHNDKPQQLKRQQLPEEGHSRRPTEPMLKRRRIADGNKGAVAATAAVMATAAATATATVAVTATATVAVTAATVVGTSADAAAREERVTSPGLIPAPPPRPLSAQSMDQPQPAAAASGAAARAAAVAVEKEWQGPHSAAVAVAAAAMPSGLWIREGRGPFLPDRIAATKTSSARSSASASAVKGSSPGALVGRKEQSSSSHPHECSSLRFRNRGAAAAMLTAADGEAGAATIASSAATAAPLQPQVLPARNPGYGGSTGSIPPLPWPHTPHAAMNPAAIFAPAFTAALAAAITTGGGPAGSSLVMMSPGQMVALTAFLHRRTELLKQQLQQLQQQQQQQNQQRQQQQQQPEAQPQQQQQPPQQQQQQPPQQQQQQQPQQPLPQPEAQPQQPVPQQQQQQQPEAQPQQPLPQQQQQQQQQAQPHDVHEPKQPHESRLPSLPPPLSKQQQQQEGMAKIRTLASFPVASCPSAPSQSASQESSAGPVALAPGPAPAPAPVPEPGAKAAEHGRAAAHPSPAVAVSKPRLSVTERLQLVQQRKAQGAARRRGTLRPGSQGQIASVMSLAGASGRMAGMKRPIGSVYVTTQMVDEDLSAFLDTGDCVGSTGASNVNAAGKMGTEAAGTAPLEASAGANPNNTAVPALAAPVEALQPRQSLLIPSLHEEEPLPLLSPVALLPPPLPPLPSSLQPSSSSYKPARNGAPPEWAGVLGSAPEVDTGDLSVRAPNHHHQHQHQQQQHQQREQQSATMASEVLSALALLQALPPPAAAPASPLAAAVPEQPSPSAASHPTASTIVVHETLNPTSTSAVHEIQEAIGNAAANPPHVTLQYDFVCAVRCICCSRPCHVGCLAGGEAAGAEGKWHHQAGNQELAATHGSNPQPLLRPSHLTTLCPNGEVPWVSTAPTPPPPPPSLHRPPGNSGLQRPHNRAGDPHGVVKQNAERQADAVAPAWESSRVPKAVGNGEFICGSECRALMRALQQLRQEGCRRLELDCKGEVLLGPGGRSSSLEWQLVALWPPGGCSSAGVSSVDDDVAGGGGGGGGGGSNGGNGPGGEEAVATGTSSAAGSGAMDLTAAMVYDPRTIQQDLEVCMGVFADGTAAAAVSSPSPSPPPPPVAALDPAWRSAGGLLGGLSWAPATGVNRSDGEGEGEGEGAADARSGRSIQGTERDRNGGGGNGVQLAALLWVEERVGGVVLVHVYGPSLAVVHVPAVHPRFGSYGPNLGALLLCTLESALSHAGVRTVLLGLTPAAAYGVVASRPKLLCKRRGRQPRLSYAEAEARASAAVAAAAATWLDGLGYTPADAAVLTAVAVAHAPKLPPPSALLSARDPRCDPPGPSCAQPVGRVLYYKHLRPVAVADAAAPAAAGAAASAVPPPPLPTSPPDVARSAAALPDASLFPVLEPLVDQEEALRPDVTVALLNGLPTRSAAHVVAPTPMELPYDAAPHRLMEPSGRNAPAAESPFACPTAAAYVACHMETPEAEAAIPAAVEVAVAETGLEAVATETSALGALAPSQPLTTIASECATTSGAFHGFPFPDGSGSFRTIDTYGTPYGLELATLMCSPDSLLLVSGVNLTTPAAPHHASAPSAAPTTTAAVDPAAMDGGAFGATHGSMRHPGSAAEEHVLESITAAASAGVQDQLPPSSIIGEIIRTYFEALPAPPPAPPPPLPLPSLPPQLPELSFEHQTLTEGKSRRRAEEARISVYDEEAEGAAQLERQTQVEVQVQVQVQEAEEEFRQHSISSEEAAQFEQTAQSFNLLLGGEQPGQMAQLSPSTPHLDAPLAHSDETAPPGPPAHSDEWASRREAGAQAEVEPEGAALQRAEGWWVLGDARREDQESHGEEADHLQPPQVMPPLEPPPPSVKYPRRLGLSQVHAQSQVMPGRVGHSEDVPTQPGEGGQLLRHVQMAVEGEDRLRKDVHVATTAAAPPPPGEGLPPAAVDGTAVTDVAFAVAEGGILAAAAAATADEAAVPTADGVDAMDRAVLADAADAAGECANRDGVNGAANGGVRTIDGVARTAYGTAAYNAATTADGIVVMADVTDAGGAIRTAEVTDAGGAVRTVEVTDAGGAVRTVEVTDAGGAVRTVEGTDAGGAVRTADVTDAGGAARTVEGTDAGGAVRTVEGTDAGGAVRTADVTDAGGAVRTVEGTDAGGAARTVEGTDAGGAVRTADVTDAGGAVRTADVTDAGGAVRTAEVTDAGGAVRTVEGTDAGGAVRTVEVTDAGGAVRTADVTDAGGAARTVEGTDAGGAVRTVEVTDAGGAVRTADVTDAGGAVRTVEGTDAGGAARTVEGTDAGGAVRTADVTDAGGAVRTADVTDAGGAVRTAEVTDAGGAVRTVEGTDAGGAVRTVEGTDAGGAVRTVEGTDAGGAVRTAEVTDAGGAIRTAEVTDAGGAVRAADGTATGGSADGTAAHLNLPAIPDSTRSAAGVVAAPSIKGGFEDGGSAPETAGPAANTPAAVGVAGEFVRAAEAPEDTRRTHGVMNQDIDAAPSGEIAAAEANPAAESLTAANCDREVGLATAAAAAIAGVTAPEVVSMAAAAAVVALRSNLRGSPIKEAGGAQRPHIQSLSREGSDAVLPVMSGSTGSVVLCGNGSTVADVAVDAVPERRKRGTENAAASGAAGKLAPWALSNTHHLHQLPASSVVRGRSAVRNIVPEPSGGEGGGGSTSAETSSSQRAEAVAPLAEVAAAAEVGRSTVAAAAPAAVVTAAAPALEGPCEKDALRQRSSRTTSPAASLAVNNRGNVTVVEAEITYGGDKVGGADGNAGGVVHDVEGSGGGAAATVAAAARIGCSGSSAPSVAAVAVGPVAPPGSEGNPHLAIQSCFSPKTGLPPHFMSSVLRRDPDVDGYGGGRVNPKPHAHGRFSEALAPTGTKAEAKTDPRGVAAGDGAGSLGMDVGAGSMPRPRPTTLATRWAGMSQHVSCLKDLQQQRRQQEAVGEQDLEVFREEQAGSWRRRRVQWRQEDRHDGQVRRASRSPRRGRDGCHSPSPRVRDNGEKNGQRWRSRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Y4</accession>
    <name>A0ABQ5S0Y4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="169" mass="17644" checksum="7A094119E805757A" modified="2025-10-08" version="1">MRRPGGQEGSHELLPGLLSRLGELRQGLGVQCCPERVQVNVTDPATVQGLARHHWVRFEQGASIAHQGGILWEQGPGREAVNALAQGSQLGWGGLYKGEVERKGISQDLLGDMDPAYEAQAVAPPHSADVVQPLDSAGVAEVGRLGDVGGGAGSPLGSCGVCSWPVLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S127</accession>
    <name>A0ABQ5S127_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005867</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="597"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="830" mass="83951" checksum="BA39228073ED9480" modified="2025-10-08" version="1">MEKSPQRCHSTATTTLDSYSMDRVVSYSVAMSHSGDSNPSTRHASRHSQFAAAAVADDDAAQAISHKQACPISNLPEEVWLLVLSWSGSRSAATAASCCSAFRALHHAVVTTPMLLARALIVDHGPFGAAANLYGGPNGQRSIRHGVHSDEGLALVLRELVKLTAPTGGAAVRVAALTTLAAHAAVAAVDMVTAMEAARDASAGGSGTGTCAAAGVTNARRLSSILAPAAPEASCADVSAIFTIRRLASGILCSAAMAGHEHVVATALVLGASGMEKALVDASGAGRGRVVRLLLACGTDPNAAVDSFGPCALFKAASNGHTDVVQLLLDAGACVDSTCITAPPYNDRVAATVLRFDAPAAVGQNRSTPAYVWGQWPAMLSLDSHRRVGATTLAGSCGSSSGVFPPLQLSAPMAGSTVALAPPQPLPPFPPLSALSAPQLELQIPCCRGAVNVANVKVNRRMPGTLGALLTTAPSSTTAATATSIPLSGASTATKTPASAAAPYTAAANLQPSTATEADTEASTTAKTTAAAISRGLPLAVSTTATSAVDAVAVMQTPSSSAGDVTPAPTVPTTSTTAAVPSVTLSCDGDKHDFLAFGGGTATSSPRTPVRSPTERMLPLPPPPPPRHRKSPGLLPAQMPSLPLMPPLPKLPPLPNTSLPPLPPGATMSLLGPSSRFSRKVSLLLVACQGGHAAAAALLIRYGAKVDREGGSELVQAGHPDVVDELLASVPDLRVHLDQALVASASDNRLAVASALLRHGADPSFGGGLALQEAAFLGHTTVLKLLLESGGALLRSSGVLKSVLEMARKWCRPGVVQILADAASPTTYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S161</accession>
    <name>A0ABQ5S161_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02883">
        <property type="entry name" value="NUDIX_Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051325">
        <property type="entry name" value="Nudix_hydrolase_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340:SF0">
        <property type="entry name" value="BIS(5'-NUCLEOSYL)-TETRAPHOSPHATASE [ASYMMETRICAL]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340">
        <property type="entry name" value="DIADENOSINE 5,5-P1,P4-TETRAPHOSPHATE PYROPHOSPHOHYDROLASE MUTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="183"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="387" mass="42393" checksum="F7FE44EFB1CF48A0" modified="2025-10-08" version="1">MAGFVHGLSVHGPRAVITKELGEMFNQTLPEDLTNLLTWVNENPKHNLLTAVVAKPAWDYPLITALRSLGRTCYIIVWHSDATEDGIARIQAFQSGANMVTNNPEHLHEALQRITVIHGTGNFSCPWCELGGFSEHELWRHQPLYHIYEPDKLDARCPACRNWTSRLTRHINLYHGPTQLVDERTGVFALAVVRRPEDGKFLMVQERYGEGYWLPGGGVDTGESLRQGAIREAWEEAGADIAVTGILTVESFHHGSWRRIIFLAEPVPFVGDRASGHRGNGGAAAAVLADPTPAVSDTDSAAVRAASAIAAVEASGSLWHRYRCKTLPDLESAGACWVTVQELEGLPLRNASEPLTWIPWVAGGGRVLPVDAGAVPEVARVFPDFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1J4</accession>
    <name>A0ABQ5S1J4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (adenine(58)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.220</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_006195</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.220" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF2">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="9">
        <location>
            <begin position="76"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="266"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI63289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="423" mass="45300" checksum="77ABB127CE8B76CC" modified="2025-10-08" version="1">MNFLRCTDITAGDSSSSSGAIREGDCVIVYEGINTMKAVYVTAKGRFDNRFGIFNHKSWIGKPYGSKVTSDSGHGGWVMLLRPTPELWTQVLRHRTQILYLADISMIVMHLDLQPGSVVLESGTGSGSLTHSLVRAVAPRGHVHTFEFHAGRAEDATGEFKQHGLSKLVTVMQRNIEEQGFPETLHGGADAIFLDLPAPHKVVPSAAACLKPNGRFCAFSPCIEQVQRTTEALSSSGFTDVITYECLLREYDVHRGLVYTGVELERPQPKRPRRDPKPSTFAAAEQGISEQPREEASASAAEESAPTEASVKVEPPAEAATTGFSMQQGSDTSGLEEEKAGISGTGTDSSGGEGQPPAGATAEAVKPPLGAEVGKLEQQQQLTPLGVGGLGVRQALRMVHCQPAREARGHTGYLTFARKFVCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2X3</accession>
    <name>A0ABQ5S2X3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="680"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="766"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="880"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1238"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1786"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1870"/>
            <end position="1926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1945"/>
            <end position="1968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2013"/>
            <end position="2073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="772"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="798"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="884"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="897"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="909"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="921"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="971"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1111"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1296"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1870"/>
            <end position="1879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1895"/>
            <end position="1916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1949"/>
            <end position="1963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2033"/>
            <end position="2048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2050"/>
            <end position="2062"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2073" mass="219963" checksum="F1792660D84FD633" modified="2025-10-08" version="1" fragment="single">GSVSPGGGGNGSRLGTAGSLPGTPPSLKVVDMRSYMPRNDKDESTGPDERLQDADVVDLLMGLMQNRQVAASDSTTFHLAAPALLRQLALDHFTRLKKSCATPLPWVLGQVIRTEMLRALDSGPNSLTLHQAYIFLTSEHSAPQDLHLSMQDMRLGLAQVEQPFTVPLLRLAQFIRQSGLLPRLLSRIITHLHKVAEYYLDRLAVVDQVPWWWPVEVLHARRAAVNSSAVSSSLYQRPDVFAMWQQPNNSNLLGLETIAGVVYDFDILGVYYGTTRKALRNQLTSEEQLALEHAKRDRLLAYVLAYIMGQYLIPVDKPWDMRPENVLTSAVSYPQFVEILAACLAIKAATQTPTGAVLTSVQLLPTLGDTLQHLPAMLESVGLWREKILYPWKFNRVKHNDVELQRQVDRAFSLYCTIDPLAQTLGINIYQFTQLIRDTDVADKTLTMERIQEIFAAVALVRVRSVPTLRSKVGPARQDDNMEWTGLTAAQIIRLSTSQFAYTVAQHKLEEHLTLREKAMAAWKPREGAASLGAGTVSLAMALREAAQRTAAEVDETEAGIVRPGAAQAASPARSAWSINSRHASVPGSLATAGSVGGVGAGGSGGGGGCGADCGGSPMGTPFQFGIQAAIAQATTTGTASLASSSVFVSKLRRPGARRSGRPRDLAGLLRNRRQSGGGLVSFAAPLDDSSDPYATGRERSSTHLGAGSSGGPLISQDGSFAVLAVGSSTNDGAFGVGLATEDEIDMRMSWPSFTTDQFAESDLESAAGGDNSINGSQPGSRQVSPNRGRSPLNGYRRIGGGGGPPNGTGSGTGGGSASAVSGGEDETLVVSLSGLPHPALELATAEVLGHAASGANPKDQRSRPPHNYTQVEDIFRMLRTPEGPLPPPPPPLPGDRLTDESDREGGAGEDSGGGGGGVVAEGAAGASPLSPRSGSRGRRRMIRRGLSNVSTGMPVAFGSGTPRRTFLDDAAAARGEAGAGGRRSSPGRGDSGLSGIDGSLDGEPRRQVLHGRSHLSLGSRMDDHVSTTDGSDYSSGSSPDRNRSLGRWMSADRNHPAQRGPPSPLGPSGGQGRIDSLRPASRAGSVRSVRSRASGLDVLQDSLASPPRSPHSQRGGSADSDSVIGSVNRLVLTKHALATTGGSKSAKKLSAQPGKLLSREQFSEVIQRLAVVKYSKISSPMAAWRLLIERHVLPVVETRSTKFDRYLEEMMGPSIMSLVLAWEPQLKALFRIYGKDESKQTTGRGPVHNRLRTSASGRGRSLARVRSSSPSRPNTARTSLSSGGGGGRARSPGTKSANSSRHGSGSESPDGGRGLNRITMSFVQFLQLCQDRKIIPALIQPAGLEEIFRRVNFMDGVESFIRNMAYPQFVDAVCLTAMTIYHHPRYKEQAMTVEDMLETFFSQLCALKRARTKAGNLGGMESAAAAAASAARGGGAVENPYAQWWSLRFETSAPEPGDHVPDWSYLSSTEYAPVLLQDLVVPPPPVPEPVARMLARAGELHNRGDCPASLAEYEKAASAWRAAISGEGHLPFGSSLTLSPEQEIYLCLVRASVLVSCGRDNSALLQYDAAEQQLTRLPEGHPAEPLIHGCRGHLLYHMGQLQDAFERLVQAKVLREQHPEMGSHHVDTAQAHHNLACCLDRLGKTHLALRLLTGAVETFRVVLGGSHPRTLTAARNMGHMQHRLFKLDLRYRSVAQEQADAEHAAVLAALHRAAGRNHRGRRPGVDGVYTLSAPRLVLRREQANAALAAAAAAAAMLVPRRLEPLKMPAKDRLAAFQLRVHGGGVTSSANAEGETSEPAAIHSSHMRSRNGVKYYRSRHDMDYLGGGLHKAHPEVVATYEALKADIAAHASGAVHSRVPEGVIVVQRGTGGASGGIDGSGHRRGGGAAGGAGTRSGAAASTAAGPAGAASRQAATEEVPGPMRAFRGGVYDRTLAPRKLKVERRYGLLPPPPPPPPPPPQEPPGRERGLQMRRANVLAAAMLEELNARLTGYSQQPTLTMVDRLADVLTGLPASSQAPKPKSKTAAMASGNGAAGGGGGAWGVGGFGSERVRAGEDGDSSSRRSGRSRPSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3W6</accession>
    <name>A0ABQ5S3W6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00481">
        <property type="entry name" value="Ribosomal_eL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000231">
        <property type="entry name" value="Ribosomal_eL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_eL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002172">
        <property type="entry name" value="PRK01018.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00709">
        <property type="entry name" value="RIBOSOMAL_L30E_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="110" mass="12001" checksum="37706E516BD3F212" modified="2026-01-28" version="1">MAPATKKTKKTQENINARLALVMKSGKYTLGYKTCLKTLRSGKAKLVIICNNCPAVRKSEVEYYAMLAKTGVHHYGGNNVDLGTACGKYYRVSVLAITDPGDSDIIKTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S410</accession>
    <name>A0ABQ5S410_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 186</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009724">
        <property type="entry name" value="TMEM70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045325">
        <property type="entry name" value="TMEM70/TMEM186/TMEM223"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13281">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 70, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13281:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 70, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06979">
        <property type="entry name" value="TMEM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="35146" checksum="7158A7362E6C3852" modified="2025-10-08" version="1">MASPRLTSFARWSCGASPLRDAIAASLWGKLPFKALRLQKAGHGQPEEPPPPSTSAPQSLTVYQLRPRGTAAFHSRGPSLPCSEAISVLAGVDTSTSSRNDYSCCTSSFRSFASSQFSTEPLRAPPTPSTTTPSTATPTPHHHQQQQEQQEREQGHLPRPPNDVAYLGPLSQQHKLLKRISIANTVVAAAAAPAIVAFADNISLVSRYGLAASLVLFGLATTGALHWVANPYVHELRYTAVNGQIDVRTTTLLGNSKWHSFNVDDVQPLPWNRPVATFTARNRFYYIDVYSFPDEQLLQRLTPDEVDVPKGYKDKEDDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S532</accession>
    <name>A0ABQ5S532_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046696713" description="Secreted protein" evidence="1">
        <location>
            <begin position="33"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="21448" checksum="C53A5352C6B652C0" modified="2025-10-08" version="1" fragment="single">EGLQWRLVMFTRLHLMPFLLLLLSSNPWLVISSSVAMGREQSQTVAMTGASFCRQAASEMRGFLRAACMVLRISVGHKLDGRHGTADCPCNTVDADSKGDKQIVGDTPPVAENSNGRAKSCSAATKLFGVGVPNPSARRLQQVGEVLNARNRTQSLSPLPRSQYRSPACSCAWSRGAAGCADATRLICRLAPTSKICEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5B9</accession>
    <name>A0ABQ5S5B9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35754">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35754:SF2">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="279" mass="32575" checksum="70A258424F76E86F" modified="2025-10-08" version="1">MAQRDDPAAITTISFKAMQRARATIEDFSRSYFPYVGLSLPDDFFKYLDVLVWVEATIYQLDEDNEQLTETGLIDHQPTAAGIKGICAVLSQQDLMDKALQWELHQGLRYWLLEQDICRRLLHAPRPLQTSAQLTAAEVLECHAAKSFDYRVLCLLLFRLTKKPYDEALLSFLRLDEMLVDISDDLVDYEDDVLANSFNIFRCYIQLYGREAELRLVERISSLEEQHGRLLAGLTEDMREHYWRRHREASEGQGSDRWVFPPPIYDEATYREQIKREEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5Q3</accession>
    <name>A0ABQ5S5Q3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Mediator complex subunit 20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MED20</name>
        <name evidence="6" type="ORF">VaNZ11_008613</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Mediator complex subunit 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013921">
        <property type="entry name" value="Mediator_Med20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12465:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12465">
        <property type="entry name" value="UBIQUITIN SPECIFIC PROTEASE HOMOLOG 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08612">
        <property type="entry name" value="Med20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI65161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="272" mass="29442" checksum="3B3CF03CBAE100D1" modified="2025-10-08" version="1">MGVKILIRSLPPKGVAGPAQHDLLKRLVDELCASQLAGRLEAWSVTCQLMKQGHGLVGAVGGPAANATSGGLKDMWAVSFRDAPEMVFLLQRPEHKVLECEAAMMRLLEKKLEYAKQMTVRFEGHTYSKGDFLIRLCTATQAIHSTQTLLGHCMELEYLPVSNMASSEGMLSEFVDLLRQVLAVVDGRQGSGSGGSGGTGSASLQLEIVKPPYDKYGLVNIPYGRAHSAVAYTDLVMMMLSSSTAAAQQQQQQQAQQQQQQLPPSTQQQIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6C5</accession>
    <name>A0ABQ5S6C5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="475" mass="51076" checksum="5D3714C08BE61585" modified="2025-10-08" version="1" fragment="single">MTSLLPSVSVGRAGSPSSTLKAGSVGKDRTQGSVSMPISGNARKTHNDLDFNLGNSKARVLKLLDDRTPTPFKKFFAGPLYDSLLNACLLYFTSRFMHDWIVKAMDKARKQHLEGYNPYAVAQRLLELEEEMEQQRLELSPIYSQIILKYSSYDKQQQDRMFFECLYDTLVNILCDAFCSPKMTGQMPSGSAAPSGPTAPPATAAAAAAAGAAGPDGSAGSLAAELAAGRRAEIESEIGALFRSRHFNSNQRKYEAPRSVDTLGVKELYSLKHETTNRALNAKMLSSLYAKPPSLSVQVASVTNSPLIAQYISSPIVARAKVKDPLERKHMFESLARAEKTSARARVSKAFRSMAAAKGVDVNGIKENLQNLKADAQAAESLGLMPAERSYRSDRRTAPRGILAPESIGLAADEQFNTLALLKRYILYGPLLGFAPPNGAPGQQPPGVGGFPTQQQQPGAGSTNVSMSGTSAPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6C8</accession>
    <name>A0ABQ5S6C8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044241">
        <property type="entry name" value="TxlA/HCF164"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47353">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN HCF164, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47353:SF1">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN HCF164, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="102"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="267" mass="28643" checksum="EB0E369943134D2A" modified="2025-10-08" version="1" fragment="single">PIKITATYIFVPMPGRLRMQVAPRSSFSPLPRCRPARTWPISYYLNRPTRGLSVALRAENTTQTTTQEVAVENAVPLESAPGAVVLPSAPETRKGNTGVAVGAVAAAVLLFALPRLGGGSPPTLATLEQMSTPLDVALVNGRPTLVEFYANWCEVCRELVPDEYELEKQYQGKVNFVMLNIENSKWAPEAAEFGVRGIPHFVFFDKTGEPLAAAVGRMPRQVLEGNLEALASDAPLPYAGAQGRTSSPAPPPGAMAGPKQTMPRDHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6K2</accession>
    <name>A0ABQ5S6K2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-dependent Clp protease proteolytic subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008380</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381:SF6">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT-RELATED PROTEIN 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="414" mass="46770" checksum="25EF929667D0150E" modified="2025-10-08" version="1">MQSLLAFRTQKLRTAAPIQCPPVNVPAPVFQRSGRQILMKNNWRTFGPTAEFSDGDAEYYQLTSRLSQQYEWFSPGQRQAEEEQEEASTSTPNIVQDKREASFGLSEKQIRALGLAGTPKDLPDPKTLSSKAYMCGEKFQPEQFGYNVANMAGGGYRAGRGYSSPRAAYGDYPNYPEGRPIFLPEAERFGNPPDLPSLLLQQRVIYISMPFVPSVTELVVAQCYYLDFDDRNRQRPIYVYLNSTGCINDKGQAISADNEFYAIWAALGFTRAPLYTGVTWKAQNQAAVLLSAGQKGHRYSFPHAKISTAPPVMNRVFGQTVDAQLQAHELEYATKYYAAILARSTGKDLETCQKQYLSRKRYFSVKEAYEEGLVDKLVPGFLLNRFRKMQQDRAGGEEELFSLNKPKFKFTRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6R6</accession>
    <name>A0ABQ5S6R6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009167</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="402"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="807"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="986"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1017" mass="105315" checksum="1377F9DAAC9D715D" modified="2025-10-08" version="1">MEHFSKALAAVSKVGEVISRTAQSAQELLRDQELRSKGIQTVITGAQTRLQGFQNGIVQRAGRIQERLLDNRRQLQALLQSLEERFNASQRAWQLQARRRDRVDVQFGQQVIENAPQEIRARLWYVLLENPHLAAPLKCEHPGVAHYSLLEEEIDGEDLLVAGGGGAGGGGSGAAAASPSSLTRRSAGGRDGAQGSAGSISSTGSRFAAAAAAAFGFNRSSGPETSLANSPSATSFTATRRDRSVSGRSGVVADTTLEATPQPNPQQQQQQPHLRMSAMISPQTGRTLRAGAAAAAAAAPAAEVASAAGAAAAESGVGDSLDGWELVSGVLCTTDPSGLPGPGPSASGTSGMLQPEAIRIIMEHLDALGPVALDESGSDPVLVAQQAVLEAMLQVPWESGTGLPTAIAPDSRFAVLNEMTAGQEEVDDSILRDIHRTFPEHPYFGMEEGQKSLFRVLKAYSLHDLEVAYCQGMAFMAGVLLMYVPEETAFRLFCRLMDAEGPNLRRLYLPGLEPLKAELATFELLLTWRMPELAAHLSEFGLPPVLYVSQWLMTLFATPFPPAFCARVIDVLLQDGHDRLLLRCSYAVMEVLELELLARHDFEALITYLKMEPLTWELGRQRAIMERALTSPLTDADIDIARDTVANRKFLAAALAAEAAASIANTELSGGGAGSSAMAGDLLGGPAAEAATATKAVNPAAVMHQRERSLEAEVDAALALAAAAALTATVAEGGAGCNAMVGVEGLLDGGDGGGGGGSVQTVGCEATGAATAVTPVLPNLKTCLSRRDGAGNMICFDEDGTSGSVSGAMAAAGSGSTRGAGGGSSVCRGTSPQRQPGRGWEPEWPVGPPHELGHRLAGGAQETTRATAGCSGMARQPGGSRPPSYGMSTSLLDDLDFGCGGDGATVRPKATPDLEYGEYGEMVMDAGLLLPELGTAGTVDAPPTAVAVAVAAGASVAAAAAVTGNGGAEGFFVPLGTGLGVDAVEPARAGTYGNPRQQRHHPHPGQQVQSGTDLLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S767</accession>
    <name>A0ABQ5S767_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="3">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_008770</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="405"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="420"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI65278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="618" mass="67768" checksum="F8E033E6F5E97E67" modified="2025-10-08" version="1">MQLQQSGCQAFERASQRRERRCVSLQGVRAVVSAGSQPCRGLGSGRQHMVCKAVATSETSVGSAVTQSGVYYNMDSSTKTSRPRLVVLGSGWGAMSFIKGLPSNIRDMYELIVVSPRNYFLYTPLLPAVATGTMEERSIVEPVRNFIVGKGDFYEALCKDIDPVAKELVCCFPEDAGLDSACFKLSYDVLVMAVGSVNNTFGIKGVDQYCFYFKSIEDAGRLRARVSECFERAALPATPEEERKKLLTFVVVGGGPTGVEVAAELYDMIECDLAKLYPNIVKDVSIQVVELMDHVLSTYDRAISIYTAEQFKRAGIKLVLNSRVAAVEDGCVKVLNKANDVTEIKFGACVWATGIAMNPLIRQLQEKLPGQSHFRSVLTDECLRVKGSDGSIWALGDAATIDQPKALDYAEQLFDAADANKDGKLNLQELRQLLNDASKEFSHLEEHARYFDSQSGIRRFGSMLANSNAGASTQERQPLAPLLTNLDTEITLDQFHEVLGKIDKGLRALPATAQVANQQGKYLAKVFASTKITGSPDQDKAVAESVKPFRYFHKGSAAYVGSDKAVFDLPRIGPLTGTGAGFVWKSYETLSQFSFRNQCLVAADWLRTKIFGRDISRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7K1</accession>
    <name>A0ABQ5S7K1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_009021</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="21"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="606"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="829"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="903"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="954"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1017"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1425"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1509"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1829"/>
            <end position="1872"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1334"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1769"/>
            <end position="1803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="657"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="835"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="903"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="955"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="971"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1047"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1477"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1523"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1829"/>
            <end position="1839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1844"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1912" mass="198119" checksum="561126D80673B866" modified="2025-10-08" version="1">MQMSADVPAVTLSAGLGASTPVLSLLHIRPLLPTEQACCNIVSVGENETVDVHGRRYAFDCVVGEGGHSRKHMCQHYIRPLVRNVCNGVNCAVLAYGQTSSGKTYTMGIGREQLRALRNGDDEEVEDLVIPFALRELFAFVDTANGSRVFTLLVSFIEVYMEKYYDLLAGRQPVAAKTRSDGEVMLVGAQLRQVKSMEDVIRLLQMGGLARITKGTTQNDRSSRSHAVLTIHVQSRAAAEAQPTTPHVSATATNSPWCSPGPLRAASASCESSGSPEPSTCTSVALPPVRTCAAVDSGSGVAALSAAMAAPPLAAKLHLVDLAGSESVGRAGAVGLAAKEGNTINLSLMTLRNLIQLLAASKASSARAVLPYRDSKLTMLLRDALGGNSSTLFVACVSPADANAAMTMSTLEFATTARRIRTKPAVNIGGGGSAEVLELHRIILKLRNELEQLRRLALPASLSPQRSPRGVMAAIRTSMTFSAAVAAASMQSPSVPQLPDSSTAPFLVSALSVGRRSCTTTAGGTPPLPLLGRISGRGMSSAGSTLAAAAVPMVAGRTASPDLAQAWGLSGRQAFSSLKYACGVKALPEENPVEAVPNPVIGFGERGSNWEDVGGPQTTADGIDGDHRRLKHTDHMHARQGYGRMGEGTSEKGAQVVRRRRRRRKVIYRKAGVGKSDDERDSPCTSGRNSPGDGSVRLHPASSSGGSASRGTSDNDRDSNAVLSASSYEGESGDSEDEWQRVARLQQRIMLLEAENEHLQKHVGHLSSVVSELRAQLDDTLAARVASPCALSITPGMSRPVHSSASGASLLGQSMLSATTARLQRLGASAGGVSRSGTSRMAFSPSPLQDSPYSAFQKSPPMWDRAIRMSCGDHSGHGSSSISGPRRTAMGCATVTTTAHDEAVQSTQEISSLRGSRAGVSEGRLGSPFTARRRYISDNGIPASTASAYVAMMTQQQQQQQQQQGLVPAEEGGGGGGGAAAAGAGRTFLASSPLAPPAVGWLAGDATAAAAATAATAPPAESGGSNACSTLPQVVEEPPGECEEAEVAARREEQEQEHAGNNVPTAAAATPSESLEADSAGDAAMSLVLSEEEEVVAAKHRGGLGGAGTGGGSAGEWLAAAQEEPALAGPQLASGSDGTRMLSMLSWIGQRVVDSESLLATQRAELRALLSEQGLTVNSTAVAVPNGLPSLDTLQFFAATQLAGVEAELSGLLGSAGPVPSCNSLSGEIMAEGSEPALTGEDESPAPAGTAAVTAAGTAAVLEEVSEDVARRIELLQSRQERLKGLQTGLKRVAELEASLELQREVQMLVAVYAGLSSGDDPGVATGPAVARAAAAAEQQLQSLRRRRRQQVVKDAAVAREKLRELETLPGPPTPLEFGVLLQTAGGSSGGGSCTLTISTSGPPSLTLSTSLRSMGAASWQPRITIGGLSSGQQSLDAGEDDDDFEREGSSGALTPKAGMTAGSGGGGFTAATLTPTPTPPLPPLPPQPAQQQQTPSLLAHLLPALSPISTWRRGGNKDAAHRQQQPHSQPSQQPHTHQRSGTASGPSSSQRRVVVQTSLVGPWLDALLDYETRLQQYTRVLAGALLEREAVYAQLQEVEQDLAVLAEAAAAQTLAGTGTAAVVPLTRSHSSSGPSQGGTRTTGRTDCCAGLGSLLLSGRTDGGASLASWMTSPMRAMLKGADVWVQQQAATLSARQAKLQKRLEELCMEVASLQPAIVEAQESRSAATDRDDWWTCVPSLSLARPALRMLLERAAEYRAAFLEEMSRADKWEQQVEELGLQYQEAEARLAALSLELTATRAAALAAVAGPGLNRSFSDAVAVLLQQEQQEQQPGQQPGRHGDGGGGRGGGGGGDGTSGAGQSGGTSLLPSASTVRASGIHPVTEALEAAVMDVTPCGASDRLVRAVPWVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7S2</accession>
    <name>A0ABQ5S7S2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="391" mass="40408" checksum="419942A0FECCA96D" modified="2025-10-08" version="1" fragment="single">NVSGFAFDFQIDFRAFPQLVQIVNIGNADSKKHRITDGISDNLILCCYSLQFLMVTFTRGDSPDAAKRLAVLSAYAAQGPELTTAYNTSLELLEGAADLRNGNITIDVWPLLRNVTFDPNSNISNVALPVLRRLNDTVINVPWPQSPGAGAGAGGGAAAAAAAAAATSPTVSAMQGPTPSSSLAALAAAVAAGRNVAKKPATAPLADPDVLDSTATASVEGSASDTMTPIVKKETAVVMDGGAKMPPNANATAATPKEANAIESPTTPPPALTPPPAPTPPPAPTPPPAPDMENVSMNFPFQVVSLFRAHGDGSKIMEPIGKRRVTNNTAAGAASLEVAEGSDWNIPDRRPATVFYGLVNMADAYIDISRSGVYSIAANATKEAQNSYLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7Y0</accession>
    <name>A0ABQ5S7Y0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="545"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="560" mass="56963" checksum="EDE6F1C5EEAD6BA1" modified="2025-10-08" version="1" fragment="single">GVKRVSATGTALPSLRFSSPSLAIGTCDSGGEAGTGLKQRPRAAAGGGGAVTVPPRPVSQATVAAANATYLQSSPSKSCLISLDASHVSYLGLTKIPPAGMVSSSSTSSISPSISPQPPQPSPVGLQEQQLACVQHQAAVLPPRVAQPSPNGAPATLPSIATFNDSVAPTGARAASPSPIPRPARGASPSHGCSQDIGFTGGVAAVWRLAAPAASRDARDVTPNGRSSEADASSPSALLSTSWSGGSWPYRPSSSCSNKGVPEENLTVCRSGAAFSSRHQRPQGASQGYSRTGPLLCPPPKELSSTPAAGPAVITSADAGAFARPANAPATPAHQPPLTQQPPLGQRSLTLMVSPRTAAAGDAEAQGPLTFPLQPSLVQHHSSLLPNHQQQRQVQAGCPIQAGATAPALSHPALPQLDCSMRPYSPGAPSPGQLDGNAAFVYAGSALASMLRVKHAPGSGSSSSSISRATSEQRPRHKSESSSGFFQVPHPHPYPHQHYPHPPGPRAQQQQQQQQPANQEAVRQVAATASTAFSLMSGRCTSRPSPSPPPGPLSPSLPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8K1</accession>
    <name>A0ABQ5S8K1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051363">
        <property type="entry name" value="RLR_Helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14074:SF16">
        <property type="entry name" value="ANTIVIRAL INNATE IMMUNE RESPONSE RECEPTOR RIG-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14074">
        <property type="entry name" value="HELICASE WITH DEATH DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="778" mass="83985" checksum="F395AF2E1A3FCFC3" modified="2025-10-08" version="1">MTIQISATAHPTAASFCDDWAAALSALIAAEILPDAPPVSTLRAALPKNLNPQTAQPFHDFFAMDNDSANILLPLTCNSIYDDRGIRIPVVAAAEPESAVSPNFSSLHDAVSRCVSEHPAIPRYLRPLSVLLIAAEAVRHLVRRPVGNTDGRMEATVLQHGPLCEAPSVAERLPCVRQQQHLMDFSETETTKRSHASLSTSEGDHEATIATSNNPGAAADNLDDDDDDSLSYVHVHKRRRVDEQHKVPCCGNAVGHVVVAAAPGPTKGEVVPEPTLVQCHAEMRWQDAELAAYLREFTEVVALELGDHMSGITVEGKEANEALSYLQRNLDAHLAMLLAGASDEAGTAADPTTLTGTAATVTPTTNLSSAAHVDSLRLLTQWLSKAREFAVKYPCPNLELTARLMDALVTAIDLVEDMGFEAALPYLARHAVVLCRDDLKIHGDPMRVDVTPAEGPSSSLPASLQEICRTFTAAALTTSTTTIPATSSTFTSTPMGQQPAIRYGLAPRPLGLLVRWKLSNVQGPTQRTVQLGLGPMGPSCPYPWWNPMAPTRGSDLAAGEVVAALTSCTDIPTRVSKLLKDLIVPDEGCDFLYSIFRHQHPPHHHHQHHHQQPQPPLGRSSDSDRDPAAAAVVEAAVRTCFSSWDDAGSSGAYTCPTFWTLVSYLQRYRHKPSFHGIVFVRTRQAAVLLADRVQRTRELQFLREPGRKVLVATSAAEEGLDVASCEFVVRYNAHVCGIGQRLVRDGNGWRIYSAAGRRLLRGVVEVCDIVVSGSGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8R5</accession>
    <name>A0ABQ5S8R5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase III subunit RPC6</fullName>
            <shortName evidence="6">RNA polymerase III subunit C6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007832">
        <property type="entry name" value="RNA_pol_Rpc34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016049">
        <property type="entry name" value="RNA_pol_Rpc34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12780">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED , 39KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05158">
        <property type="entry name" value="RNA_pol_Rpc34"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028763">
        <property type="entry name" value="RNA_pol_Rpc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR028763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI65586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="283" mass="31986" checksum="0075C09AD5C1727D" modified="2025-10-08" version="1">MAATKALEEELLKLLRQHPQGLTDDALAAALPHVKIEERVNAVNTLLASKRLQLLKASNNKLVYKEVAAEEAVKFKGLNTEEMLLYQCIKSAGNTGVWTKDMRIRTNLAQPQITKILKVLESRRIVKSVKNVNNPSRKVYMLYELEPSRELTGGAWYTENQFDSEFINVLREACYSYIQRQKDTSLGDIAAFIRSRGFSKVELRIEDIHTIVMTLVYDGRIDEVEPEGEDDNEDHYRPAVLVVPDSTALTDVPCGVCPVAGECREGGTISPQTCVYYDKWLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S939</accession>
    <name>A0ABQ5S939_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="103" mass="11019" checksum="A36BF6C4C717727A" modified="2025-10-08" version="1">MVSTFSNVWERQFLPNILGCALGAVCLIFGGSSPPKDVAVRQFLMFTRAPKSDVLVQTVTVRQLVVTDATPLGVLLLKATTVQQFEATPEACLNASVPVRKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9Y4</accession>
    <name>A0ABQ5S9Y4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010679</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000039">
        <property type="protein sequence ID" value="GLI66737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009631">
        <property type="entry name" value="CGLD27-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34214">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34214:SF3">
        <property type="entry name" value="PROTEIN CONSERVED IN THE GREEN LINEAGE AND DIATOMS 27, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06799">
        <property type="entry name" value="CGLD27-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0934">Plastid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="314" mass="34498" checksum="384F0EC45A06B7C0" modified="2025-10-08" version="1">MVPRFTNPNRSAGTCLLRMRPTAAEMRHQCDGRLKRFMEPRCLLRPSAPRRVVVTHASPEKSSTDEMKGGLDPYLEVSVPKDQRPVNQLAELKQDPLYSWGALEQGDYVKRLTGVYAFFFAFVGGPIAYQTFEPTVQPLEWFLSGTTGALVVVAVVVIRIYLGWSYVGDRLLSAAVPYEETGWYDGEVFVKPPEVLMRDRLLGTYEVKPVLSKLRSTLLGSAGLLFLTAVLLFGLIKAGSDSDGMYGRGAARVPRQVLADGVLYSPRVSDLSQLVTDDEAAAAEAEAQGSIPGYCGDRALRAFAGGQYCAKFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA02</accession>
    <name>A0ABQ5SA02_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000039">
        <property type="protein sequence ID" value="GLI66747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="32494" checksum="F18D71AADA41D681" modified="2025-10-08" version="1" fragment="single">LLAAATETGFRPDQLATLIAQTTEIRTQLGKYLLSLLNLLKSTVAVNTNMDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTEKTAGHSIADATRAYVRDAKKYLQAVHGGDNDGIRCLFVGNTLEGSAKDWYREWTTARGDYTFDEMIAALLARFATEVQARDVEARKVLAAGSYHMRRGENVPAYQSRFEALVTPIADLTETERVFWFKRGLTKLLAKWCATDLAGVPIKSYDALVSFAKGTEIRLLAGEEEVKSVPRANAVQVQDPLGEAVALDQELGATRPTPTAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA13</accession>
    <name>A0ABQ5SA13_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF8">
        <property type="entry name" value="SD21168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="128" mass="14041" checksum="E096B76A18F3384C" modified="2025-10-08" version="1" fragment="single">QYATDVVAAKALAMLRSAAANPGGQPFYLQVAPPAPHHSMHYDFTASGAFVSRHWYPPVPAARHWEELSGAALPRPPSFNDPWVSGKPAWLRALDPLTAANVTFLEEIHRLRLRCMRAVDEMVEALGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA30</accession>
    <name>A0ABQ5SA30_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010771</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047086918" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="38"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="899"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="665"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="826"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="844"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="855"/>
            <end position="887"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1044" mass="108655" checksum="57C28279A72DD48A" modified="2025-10-08" version="1">MARSKLPNMVLWAAVVLLSTSLVAKAQDTADVAGLLTFPWCQCTTYDCACSPYKLEVIRTTTVGTATTTCLSVYEIGCDLATACCRGMRAAVDKISFESTSKCSNKSAILNVTIDGKRYQSWDVIPRNPGFDFKIYNMGRNISTFPGSVICISTRAPCSSTNDWCFNNRTKTCRYSITESGDTNWCPTCTNTLSPQPRPPPSPQPPPPLPPAPPPKSKKRPPPLNPSPPPPSPPPFPPPPSPPPPCGADIFVQLLPPFDGGGGSYSFNDAICAVLAKQISADLSAQAAAVGSVFLDSFHLATCDPMYIRVSGAFRGPNDGKLLEPWLNSPAGMQSWVTVITGGASCTPSLYGYEVFATVIGPDAGFTCLSGEWAQDCPPPPPSPPPPSPRPPSPLPPSPQPPSPSPPPPSPLPPSPPPSPPPPSPPSPPPPSPPPPSPPPPSPPPPLPPPPSPPPPPPPSPPYPPPPSPPPPSPPPPSPPPPSPLPPSPPPPSPLPPNPEPPSPPPSPPPRPPSPAPPCPSCVKISITPPSALVGPLFTFTDAMCAQLQSLIANQMADQAATLGAVITAPFTLSNCSKTFDGSVVPPILPYITVCGAFRSADNGALLQSYIEGPTGLRTWVQALVGGEQCFPYLVGYYILGEVVGVDGAGPSCLLAEYGQDCFLPPPRPPPSPRPPPAPPSPPAPPSPTPPAPPPPCDVLVSITLASMTGSAPPAFNLSQCTSMALRIGSSFVAAAPTYGTVITSGFTLASCSSTMIVQGALLSNEDGAKLQDWLNGPNGMLAWVSSIFGGPKCIGVDGYTVLVSVGGRNGASCLSGSYNQDCSAGYSLPPPPRLRKRPPPLTTPLTPSLQPLVFMPPPQPPSPLPPSPPPFPPPSPVPPTPTPPAPAPAFPFIIQTQICRTETTNVPYGAGPLYTTATKDQNGDAVVAMCTNISTQSCSTGACCNMDFVRIEVPISNSCKSDLRHLSINGIPTNYTLGMYSDFLVTVKFTNLAATVPNPKGATLCWVVRSGNCSSPANFCFNGHCQVAIFNSNNDCCPTEYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAK1</accession>
    <name>A0ABQ5SAK1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000044">
        <property type="protein sequence ID" value="GLI66871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="291" mass="32599" checksum="DFCCF37E4BCF92A5" modified="2025-10-08" version="1">MPRRAAAMVAAAAIANCKFARPRVDAATAWRRRTQRLADLCMKLDAEHVLCKAMRRWPVAAVLETAQDWPARMFALVIRDWKVKKVARLLEAKGRIFTVECCIEWEPEYLQVLLPRLDLVTARHVLAELFFKSDDKHLSAYECRCVVEQFGPKVQRKLLRKFSERDVERVMSATVVDNSSEDDSEDEEDEEDSMEGFINDGDDDEEPVDVVAGDEEDSKDSDGGEDEEDGQSGGEIESESEDEEDDDVLSLGNGATPMERTDNGSSSSDDDDDSEGMELGSNDDDDDSDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAP2</accession>
    <name>A0ABQ5SAP2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964:SF113">
        <property type="entry name" value="USPA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="4"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="194"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="406" mass="42133" checksum="0D90F3406C4F97C6" modified="2025-10-08" version="1">MAPRRLLFCLDESKQSVRALEWTLLHLRRASDELHLVTVLPPLVYNVYPVAPVATGAAVAAVTHQWEAQRRVEEHQAAEVLKAAVDLVCTQHNKVPRDLVHTKALPAAGGASGVAESLVEYARTYLIELAVVGCRGMGGLQRSLMSFIGLGSVSDHCCHNMPCPTLVVRGEDAVLSRGLMQADEAHPPKSAQPKRVMVCCDDSPHAHAGLLWALDNTLLPNDHLVLAAVATPVPFPVLDEPATVAAMQSAQYREAAATATAAAQDVAERCAAVAQRRGVGKTKITTVSLPPGGSSGDVGAALCKHAKLEGVDLMVAGSRGMGAITRSLLGLVGMGSVSDFLAHNSPCPLAVVKGVSGGGDDGSVVVSGRHGDLTAEGHGGHSALAPMGSTLRPITEGDGSEASSMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB77</accession>
    <name>A0ABQ5SB77_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GPI-anchored surface protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010655</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000039">
        <property type="protein sequence ID" value="GLI66718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046220705" description="GPI-anchored surface protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="723"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="740" mass="75807" checksum="4893B9954CE91982" modified="2025-10-08" version="1">MFTSCCYLLVLALLISCVEVTMSTRLLAVSDDVLDEHQQRSVEDEPSVDKQDCLTALVAHIATQEEDGNSGLAGHADGNDLRIWRRCNLEVQPPTPGYGSSSSTIPPPYGQSPGPSTLPSPPPSPLEPSSLPMPRSPSPSSPRSPSLSPSPSVAPTSSSPPPSQQDDSEDIMTPPYCEATSSVTCLSVNATRGTILDAFLLTAFGWKSGSATFSSLAYEFGVLANSGAVLVHKPRQPYSGAMTATIAGVMTGKTSLYVCVSSMNRTGLAIVGPRSCRTVDVQVSSTEVSTVQLEHELTAIKNDITSTSSTISTTVDMAKRLAAIASVATTSSSTASVAILASDLLGKLVALPGNDTSSTSPVLMVFDGISTIWSLASNNSRAASLVGVSMLSEKLASQQLTLEDAQPVADFFSILVGGAMQIVNEFASPTANSGNNGAAARALAAHDVFTSIVLSAAALTRGMSNKVACNGSVVSVVTPLFAASAQCMTADLVTSGGLTMSLLTPGSSGFSEYRLRLLLASDFSVNTPFTMSLNPVIGPLCAADGSCASAGFRVTITILIDTSLLIASLGGPAINLAANLSDYRYGGTVEIVSPVVRVAAPGLPFTAIGVLGLVTIGIPLNSTSVATKGAKTARAVVRLQDLGAAPVDVSAGISSSMSTTYITNTENLGISYNLMSFTSNNLGDFVVVQYDSTATPTSGASSSLPEMGSGSAGAAELAQQPCVFTACVLAIAAMIFAVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB87</accession>
    <name>A0ABQ5SB87_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000039">
        <property type="protein sequence ID" value="GLI66743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014807">
        <property type="entry name" value="Coa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36354">
        <property type="entry name" value="IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36354:SF2">
        <property type="entry name" value="IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08695">
        <property type="entry name" value="Coa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="548"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="581"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="632" mass="64929" checksum="448E59F7EDA9AAE9" modified="2025-10-08" version="1">MDQKFASSSCVTTFLAQRSDALTGPFPKHQDGAGSALVASVALAASAVVGMPGCVIRGRSGIFRSLRYCAILSEGSSLQASGATTATAAAAGLHAHLLRSFRSKAVGCMLANAAAGAGARTGVGLRSVGRSGRGGSSSKLWGHLRQQLQLQPQREPQQLFLAPTLLRSGCSIFGAAAATAVTRVSGSAAAAVSAVARCGGRMGGQGLGPAGALPRPLLVRRMATQVTGTTRITASASRSAAEAGAGRRGLMSRAGSAGRMRAALRLQLAAAWHKYGTAAYAVGALAGALLLWRVMRGVTNTFIAVNDRMAEYGIMALAASLVLLGVLYAHWRTEINPAAVYRKAMVRLNTNPGVLEVMGAPLAGSPVRAYVVTGGGLYLSKKFRIKLRSKRVAMMFPLSGTDRKGLVSLEAKRKQGRYIWRLLAVDVGPELQQTLPAGGRTCLSAAPPQQPSAAAAAAAATQPPLEQPRLYVVGDAAAYHNSGVLGELRAPFLKALSKSRSYEVEDELEDELDELNAPKSSDVAMGRTTGVGAAVRRALASATAAFTKADAGGSGAETAAAAGKSSSGTIPQTPQTKGLEADASSSTSAAPQPPTPTTPAPGTTDGSWRNRLRGLVLGLPPAAWRTRGSETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBY1</accession>
    <name>A0ABQ5SBY1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011623</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12872">
        <property type="entry name" value="SPRY_Ash2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037353">
        <property type="entry name" value="ASH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598">
        <property type="entry name" value="SET1/ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598:SF0">
        <property type="entry name" value="SET1_ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="50"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="376" mass="40540" checksum="A31A281C72AC672C" modified="2025-10-08" version="1">MSDKARSMEPPAAGRSKKQSQSDSKGNRRRPVMGRTLAQAEEVEVKEKINLVRLRYEGVPKDGQDDPYKVLISKVHKAAQLQLTDDRLSVTGYKGFRTARGSHGAHEGALYCEVCVTRMGVSGHCRLGWCTRKAELQAPVGYDTFGFSYRDVDGSKVSNGLREAYGQSFREGDVVGMYIFLPKGGRTLEPQQNEYTKYKGKWMRIEDPEPNPEPLPGSVIAFTVNGQYQGVAFRDFNEGTYYPAVSIYTMPEQTEGATVTLNFGPKFLYAPPQPEGCLPPIPVCDLAAPLSSTLAVEVLQDAGNLGSESQGAGLQAPGPSDDVMLEQSAVLQDADNKDTAGGLTVGEPDGLSESTLPDCNIVYEAAVEGGAGVISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCC9</accession>
    <name>A0ABQ5SCC9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">H/ACA ribonucleoprotein complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Nucleolar protein family A member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011632</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
            <location evidence="1 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047479798" description="H/ACA ribonucleoprotein complex subunit 2" evidence="7">
        <location>
            <begin position="23"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="8">
        <location>
            <begin position="71"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="190" mass="20992" checksum="ABD6182BB053EBDE" modified="2025-10-08" version="1" fragment="single">GRSRYLCIGVFLFITVSGFVSAAIDTDTFFTMGKSDKTDTEKGEQGIDYETKKRFCSVIAKPLADEKLCKKVYKLAKKASKRKQIRRGVKEVVKALRKNTKGICVLAGDISPIDVLTHIPIVCEDHKVQYIYVPSKEDLGAAALSKRPTSCLLILPKPIKGGDGDEAEAKEFAEAYNEVEKKVKSAQVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCE0</accession>
    <name>A0ABQ5SCE0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI66997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="618"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="798"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="973"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="521"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="618"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="802"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="818"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="978"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1018"/>
            <end position="1054"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1239" mass="127908" checksum="63038D4378E19E4F" modified="2025-10-08" version="1">MDDASKMFKQNGVQMNSLDKLTDWSFGASELPSRWADTSGIETHHASRGKCTHNVPRLTGFEASASRHGAPCSLATGMMASSRQKSAHWPVTPEATHSSSAHNSRLQSMVPSSGPAGGVLRRTLSAVAGYSSSAPPWTNETSDSPSVPPSPNPMAIQHETLCFDAAYPRHSTSPGTLSMYKGSNPNLLAYDQACSSPQRRTLSLPIRSSPPDVTGAANLATLPGASFTPSKLMPRSARLYRPAPQNQYGGGTLPAVGAVRQYDGWQSRPAEGLDGVNDGGLQPAGAGATDWHPMEFEAAACSCSSEGYNQDSQPPQPPQPPHHFQHNQCSSHQSQPFQPPPLPLPAHVVPVIRTTSLDSGSTSFGDACSSPLKRVLLASEGTRWGSPAARSPIGVQRVLTKYTMSSGMTPLSTLASPAVSSASCALPGGTTGSPRLHPAVTAAAAARQRARSGGGCGDGSGHSLPSLERRRLMLSSVSNLAQLRDTVAEDGSLTPRNKQALLNMIEDALEEQRQQQPPQPQQSAPCPHAAASTAAFCQHDRDQPPQQYHFHQQVRQQQLQLSLMTAPQQPMLHAGGPTFRPHVQIQQPIPQVSELLSCSGSGGPVQCSTTDCSQTNQSSAWGCSPSLSSPSQTTHGSLKRPMPATALDMHSSRTQQASTQQPTAADGAYGCGAFAPGVRTGPLPLPLPAPATATGERTTEWTTSAGIAGSASAAATYQNRTASLVSHGTGGSALYPPRSSNEYANACNQVGNDAMDDCQGSDGNDGAAVAVPAGAPSATAAPTATATFPSAAAATGCRATASDGSSNRSSTTGMWGGSPTALSSFSPSSERGPVSRLGLGALLGTPNGSSPGGPRPAAANNRSAGAAVPLRNPTQVLNGGALVYDAPPEYTYGMPIAPYVVPQVCRGGSGVRCQGSGGSGGGGGSGGGGGSGSMIAATVTTAATAAPSQTECRGLEQMPFQSSQPIGSAVPLRQPLELMPPPPPPLLLPPPPSPKQQQEQAPPQPLPVSGALPTSTFQPDQSSQWTPPSPSSSLSPNPTAACTASTPSPARPAASPAPNPGQPWPAAQTGVLMPLCENPSSVPGAPPPSGYMAVDTALHLQRDMSDNCQNLPTQQQQQQQYYPSFPQHSHAPLDHQPQQQMLLHYATQPPRTLKQQPAPGLQSWLADSSEVEDPNAEGGVCAEGGNQDGVLLASDLLGGSADEDVLSEVLNSLLQEKEVMVGGFPAGEFEMNCAECYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCR5</accession>
    <name>A0ABQ5SCR5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Clp R domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011143</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="entry name" value="Clp, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF18">
        <property type="entry name" value="HEAT SHOCK PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="Double Clp-N motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="8">
        <location>
            <begin position="5"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="877"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="417"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="915" mass="99770" checksum="E0C61A06CE1A06D2" modified="2025-10-08" version="1">MGFDPKKATEKVNEVISSSINLAQEEQHATLTPLHLAVVLFEEPQGIARSATSRAGGDEAWRSCTRVLRRRLAKLPKVQPAPDSVSPGRDLTKMLSAAAKAQKDRGDAYLGVDTLLSAVIAASDVAEALSEAGVSKGQLESALAEVRQASGNGPVDSQTADANFDALSKYGTDLTANAARADPVIGRDDEIRRVVRVLCRRTKNNPVLIGEPGVGKTAIVEGLAQRVVKGDVPETLRGVKLISLDMGALVAGAKYRGEFEERLKAVLTEVQQQQGKVVLFIDELHLVLGAGKSGDSAMDAANLLKPMLARGELRCIGATTLAEYREHIEKDAAFERRFQQVLVKEPSVEDTVAILRGIKERYEAHHGVHITDRALVVAAELSDRYITTRFLPDKAIDLVDEACANMRVQLDSKPEQLDALERQRTRLQVEAAALAKEKDALSKKRAGEVEKELAALEDQLRPLLLRYQQERSRLEELRRLVQKRDEILVNIQLAEQHNNLARIADLRYGALPEVEERIKAIRAAQPSDAMLSEEVGPEEIATVVSRWTGIPVSRLKQSEREKLLELRAELQKRVVGQDAAVAAVADAVLRSRAGLASRSRGSSFLFLGPTGVGKTELAKALAQLLFDDEKMMIRIDMGEYMERHSVSRLIGAPPGYVGHDEGGQLTEAVRRRPYSVVLFDEVEKAHAEVFNVLLSILDDGRVTDSKGRTVNFANTVIILTSNLGADALLQAAQRLQHGSTGGVDPYKEAKQTVMAAVRRFFRPEFLNRLDDIVMFEPLRPEEMTRIAGLMGRELAARLAPRNIGLSFTDAALTYAVQQAYDPEYGARPLRRWMEHTVVTELSRMIVSGKLPDNSDVVVGAAGGAIGGFTYKVAPKPAAEGQAGGGGAGAPSAAELLLKRARPDTQSMDLDDNEAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDB5</accession>
    <name>A0ABQ5SDB5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05382">
        <property type="entry name" value="CAP_GAPR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034113">
        <property type="entry name" value="SCP_GAPR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="579"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="744"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="785" mass="82092" checksum="40C2E5C1522C8B25" modified="2025-10-08" version="1">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</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDF1</accession>
    <name>A0ABQ5SDF1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="6">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_012263</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Tetramer of 2 alpha and 2 beta subunits.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="7">
        <location>
            <begin position="53"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="400" mass="44127" checksum="292D71522F9ED31D" modified="2025-10-08" version="1">MQTMLKQHSGFRAAQPGRQVVKVRAVAAPLKTATSAPAKAPLVTPEVAKDLYYDMVLGREFEEMCAQMYYRGKMFGFVHLYSGQEAVSSGVIRLLRPDDHVVSTYRDHVHALSKGVSAREVMAELFGKKTGCCRGQGGSMHMFSAKHNVLGGYAFIGEGIPVGLGAAFQSKYRRDVLGEETSDSVTCSFFGDGTCNVGQFYESLNMAALYKLPHIFVVENNLWAIGMSHLRATSRTSGDETPYIYKKGPAFGMPGVLVDGMDVLKVRQVAQEAVERARRGEGPTLIEAETYRFRGHSLADPDELRSKEEKARYQARDPIPQLKKYMLEHGLATEAEIKALEDKVAEVVEDSVKFADESPKPERGQLLENVFADPRGFGISEDGRYRYQQPGFSSGTAIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDV1</accession>
    <name>A0ABQ5SDV1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012428</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12317">
        <property type="entry name" value="RRM4_RBM19_RRM3_MRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12318">
        <property type="entry name" value="RRM5_RBM19_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12320">
        <property type="entry name" value="RRM6_RBM19_RRM5_MRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034423">
        <property type="entry name" value="RBM19_RRM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="371"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="586"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="717"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="833"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="21"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="110"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="227"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="452"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="666"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="481"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="668"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="943" mass="99596" checksum="970BFC872E446EE8" modified="2025-10-08" version="1">MDTAKLAVEFARKVGDEQLPRAWSKHTTGTTANRKLTGGAGAVTRTAEDGKSSVGGETAKGKDKKDRKVKDMLLPEEVTEDPKLQEFLALMQPRFKAKIWGNDEVTAKGVAAAAAAGQQAHRPNRERGDTREHEDPDLRGAAAPRVDDGDEDDGEDDQYQDWTDVAARARRIGLGDGSEEEEEEEKMVSGSDGGEGDGDGGAGKGGRDEVVINDAVSDLEYLRSRMRRWDEEEEDEEKQWKDGKNVGDGSNLNETDRNQGVVKGATEAAARGDQTGVGTSPRCEVKAAGGGKKAGGNRKAAAAAAAAGLGLRELAHVEVGATQGGDGSDREGSHAADANGDVDMEDAAAAPPPPVMPVRTEGEAEGILETGRLFVRNLAYTATETDLSDLFGTFGNLQEVHLVMDRETKRSKGLAYVQYQIPEDAVRAAQQLDGTIYQGRLIHILAAKPASGSAVQNTGSDGGEEDGPGAGRRPGTSSFKAQREAQRKADAGNRAAWNTLFMRPDTVADAVAAHYGLSKAALLDPSATGGKGADSVAVRMALGEAQVIAATKQALAEAGVSVEALERAAAASGKASTTRSVARSSTTLLVKNLPFSADEDELAELFGRCGLLARLVLPPARALALVEFTEAQDARAAFRSLAYKKYQHVPLYLEWAPADVFTAPPVPREQQQRQQAAKAKQPESKGGISATAHEVAGIFADGDDAEAGASANPTVLGTIYVKNLAFATSDGALRKHFDGAVSAVGGSLHSAVVARKKAMDGALQSLGYGFVEVDSEEIATAVIKKLQGSQLDGHKLVLQLSRARKAASAATAGGSKAAKKTAGGSQADQDGSTKLVVRNLAFEATKKDIQGLFNPFGHLKSCRLPKKFDGSHRGFAFVEFVTKQEAQNALEGVGGTHLYGRRLVVEYAREEDTLDDIRNKTAAKFKRDVENVEDDAPAKRLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDX5</accession>
    <name>A0ABQ5SDX5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CYTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07374">
        <property type="entry name" value="CYTH-like_Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34948">
        <property type="entry name" value="OS08G0299200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34948:SF2">
        <property type="entry name" value="TRIPHOSPHATE TUNNEL METALLOENZYME 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="1">
        <location>
            <begin position="83"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="280" mass="31233" checksum="BCA0BC0A275122DA" modified="2025-10-08" version="1">MGCALIAKHFRSFAWRPESAFINAATQAYTNRIKASRQRGVHKIESSIGRAQSSSARGRLGRSFAGGQAVNSRADRAPEEVKPMEIEIKIRLPDRAAYDKVAAALEQGRTASYAQANYFFDGPNRELNSRRVVLRLRTYNGDEKATITLKGKQILENGIGRASEVEEGVDSTAAPRYLAEPTSMLQELPLIKTTAEKFGLESLVSLGGFRNQRVCYTWEGHVLELDETAFDHGTLYEIECETNHPEILRDRLESFLSSLGVSYSYSQTSKFANFINKTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE96</accession>
    <name>A0ABQ5SE96_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">adenylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012545</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="167" mass="18781" checksum="572740DB5A12B803" modified="2025-10-08" version="1">MVFYALLCNVDGDGSCSFLSPQAEQLLKSTDIGLVLNLSLREEVLVQKCMGRRLCRHCGKNYNVADIHLAASADGRPEIVMPPLSPPPECAPHLETRADDQEEVIRHRLEVYKHESKPVEEAFRKAGLVIDFELTAGIPETLPRLMPLLESYVGRHAYDRAYRTGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SED8</accession>
    <name>A0ABQ5SED8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoacetylglucosamine mutase</fullName>
            <ecNumber evidence="5">5.4.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Acetylglucosamine phosphomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">N-acetylglucosamine-phosphate mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_012480</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23804"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="5.4.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.3" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03086">
        <property type="entry name" value="PGM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049023">
        <property type="entry name" value="AMG1_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049022">
        <property type="entry name" value="AMG1_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016657">
        <property type="entry name" value="PAGM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955:SF1">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21405">
        <property type="entry name" value="AMG1_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21404">
        <property type="entry name" value="AMG1_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016408">
        <property type="entry name" value="PAGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="54"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="125"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="15">
        <location>
            <begin position="183"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="14">
        <location>
            <begin position="319"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="12">
        <location>
            <begin position="508"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI68144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="530" mass="56615" checksum="D83E12EA567F3B5B" modified="2025-10-08" version="1" fragment="single">MFDFHTVKKASSEYAKPADFHPSYGTAGFRAKATLLSSTLFRCGLLAAARSILLNAHTGLMITASHNPVDDNGVKMVDPNGGMMPQSFEAVATELANCEGDDAVVSVLRDRVFGPTTSGSARSAATGTLTVLIGHDTRPSAPSLLAAALAGVRALEVHVHSYGCVTTPQLHFLVYCANQVPAAPLPQLQSYFDVIVGAFLNLTRVGELEAGSDTLEAAGAGSNGGLLFVDCANGAGAEQLEKLVPALESIGVELRLRNIGRGGGQLNYRCGADFVQKDRLPPTEMDDVSVGSRCCSVDGDADRLVYFSPLDGGGISLLDGDKVAMLAAVYIRDVMNLLPMELLHDIQVCCVQTAYANGAATAYLQDVLQLPTVCTPTGVKYLHEAAHNADLGIYFESNGHGTVLFSKKFVERLKAVEDHPAAKELLLLSQLINQTVGDAISGILLIEFILRRKRWTLQNWQTLYTDLPSRQLKLQVADRSSITTADAERVCITPPGLQDAITRVVSMLKHGRAFARPSGTEDAVRVYAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFB5</accession>
    <name>A0ABQ5SFB5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012987</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="14456" checksum="E819DE0095A426B2" modified="2025-10-08" version="1" fragment="single">SYQLLDNRIVQDCYREEIQRIFNRSVEQLNLTGAELSTLNISMNRASGYGYLIAIIPSKLQVVNVSWSGNTSNMASNNETKQQRQRLLNSVSGFGTAAQQSYSLSDNPYLRIMPGWVFDKDTQTNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFH1</accession>
    <name>A0ABQ5SFH1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_012582</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF13">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 8" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="4 8" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="10">
        <location>
            <begin position="108"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="293"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI68233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="598" mass="67620" checksum="1D3C11324431A0CB" modified="2025-10-08" version="1">MLHQFQFSVVRLHHRRVHERVAPVPPMKHHHRRVRCIQQGLPASHRAEDASRYTARSSFTADQDTTSSTNGAALSASSPSVYSFGSLNVEEAYDLERNEWGTTSLSVVVVGASGDLAKKKIFPALFALFYEGLLPAEFHIFGFARSKMTDAEFRDVIASTLSCRISARDKCQEKQEDFLRRCFYSPGQYDKADSFQELNRRMSEVETASGKQRANRMFFLSIPPNVFIAAAGGAADFCSTRTGWTRVIVEKPFGRDSASSAALGRGLAAHLREEQIYRIDHYLGKELIENLTVLRFSNLVFEPLWSRQYIRNVQIVFSENFGTEGRGGYFDQYGIIRDVIQNHLLQILALFAMEPPVSLAAEDIRNEKVKVLRSMRTVQLEDTVLGQYRSRHHGGRTLPGYLDDATVPPGSLTPTFAASAMFINNARWDGVPFLLKAGKALHTRRAEIRVQFRHVPGNLYKHKFGPDLDSATNELVIRIQPKEAIYLKVNNKVPGLGLRLDTTRLDLQYQSAFSQKELPDAYERLLLDVVNGDKRLFIRYDELEAAWDLFTPLLHEIESRKVAPELYPYGSRGPVGAHYLAAKYGVRWGDLANEDPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFK5</accession>
    <name>A0ABQ5SFK5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_013005</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="2">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="7 9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0888">Threonine protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI68635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="269" mass="29075" checksum="685A4E7C7965D077" modified="2025-10-08" version="1">MGSNAGGFDFDLCKRNAMLENKGMTLPKAWKTGTTIAGLIYKDGVVLGADTRSTSGNTVADKNCTKIHFITSNIYACGAGTAADLEHVTEMVSGQLALHRYATGRQARVITAMTMFKQHLFKYQGHVGAALVLGGYDLNGPHLFTIYPHGSTDSLPFATMGSGSVNAMAMFEAYYKDDLDRPEAMALVARAIRSGVMNDLGSGSNVDLCVITKDGVDYLRNYEYLQAKTYTRQFPVKYPPGTATIVKQRVLSLKGVEVIEGVAEEMDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGD3</accession>
    <name>A0ABQ5SGD3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">VaNZ11_012778</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Endomembrane system</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02123">
        <property type="entry name" value="PA_C_RZF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051653">
        <property type="entry name" value="E3_ligase_sorting_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044744">
        <property type="entry name" value="ZNRF4/RNF13/RNF167_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47168:SF1">
        <property type="entry name" value="OS02G0798600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47168">
        <property type="entry name" value="RING ZINC FINGER DOMAIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047322931" description="RING-type E3 ubiquitin transferase" evidence="17">
        <location>
            <begin position="23"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="165"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="374"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="328"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="473"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="550"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="343"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="477"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GLI68392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="660" mass="68447" checksum="1D054C0C683574F2" modified="2025-10-08" version="1">MRWWEGFLGLLALAQLVELAAADVYIMTSAYILDPLPDVPADFGPDIPDAGIEGILRSADPEDACSPFTFVDPDTPWIALIARQQQLHPNNCTFDIKVMNAQNAGAMAAIVYDDVYESLIIMSKPKGHQDPVIPSVFVSQKAGIIMRKLMTIDTIRVRITPLSSVAWLSMLMSAFLGVLALGVVLATFYVMRSWSMWLSGMHIRGVTPGAGGHGGQEQAGHVRSPQQQEPGLPAESLRLLPVLVYESPAPSRGGARGGSGPASGLGGGAISSTLSQPGSLSRAAALGTAVWATAEQGAVGDEEMQPLLSDGGNDIEDGCGRYMLEGGEVEEEEDREGEGQDLASRSSSESGCASATATMLRPPGAHAGETKRVCAICLESYADGEKVRVLPCAHRFHMGCVDQWLGTRRYCPVCKHDASQPLPHPLASRAAVGGGGVGAGSSGRGAAEGVTGWTLLFQGLMDLIRHPRVYPSQRQRFGNETRGHTRDGGGQQQQQSQQRSVSRSRSRRRQPQLLAASRTATAAGPELQEPLMPVAASARLPTDTAAALAPAGDVEAPGPSRPPPAGTATGAAAVASSCPISISTNAAPNSNLQTLTAATAAAAPPQAPEGAMAGGPAIVPSGGPAARVRAVLTGLLRSAAVEVPPTASTGAAALQYPSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGI1</accession>
    <name>A0ABQ5SGI1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1253"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="674"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="979"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1052"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1164"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1709"/>
            <end position="1800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1974"/>
            <end position="2067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="683"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="994"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1006"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1195"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1717"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1981"/>
            <end position="2011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2027"/>
            <end position="2036"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2141" mass="222317" checksum="F89080B5C7847754" modified="2025-10-08" version="1">MVSALRFVRPLGILKSRYVFLLVDIGLLLALLFDGASSLTFTLASDDGSYYPYLDALLQSPNDSYIVGSSQELRTLPEYNSTTHSFPIAERKAVVGESFFSVFSASQIPLWEVENGTSYVPAYFDVRNGAELLLLGFNLNWGAQPRIGRVLNPSNSTFSNYPQLQLLLVGKLLVYVELSGTGRLQLLQSVITGAPCSGLKALFEGLNMISTSTNFLTQIGNNVKPVLRFVYPYLFIKYAQLGPIWVEDLTISCAADLTPAACVPREPTVVNVSSHDDLVSALTQMEAVHVPQCEIHTTVLHLLRDVTLDVARWPRGGGGLFITSNVTFECAPDQQLVLDFAQGLNLFKLRGAAFVRMHNLVVTNLAMAPVGIFTVPIWAFNFERDLTVSEEGRLHMVNTTGVIASDEYRRLEFWTSILTNKVQTVAALAGWQHLIGNMTLSTLTNDTVSFGHISGMGVTCVNISITMSPVGSAQSLMEPLTGVDQLLGSDIALTDPPYTIAIYSAAQLYRTLSYPDARLTRLAEADGLTNGNIASVLLLLQRNLTLDPNLWPTGVNAPSVRRRVIIQGKPFKGIELSFGGQTRLLSATDPGGSVSFRYLTLRGAGWLSDYTASSAMQQLPALEYFQTGMWVVESSRRGVTYVELKSTRLLVSAEELNVLFTCLDSYSASNNSALTPANAAPSEASSRTDNGSESVLKAASRVLPPGLQGNTCTLLIPQTAQLANTSREAGLIMVKSMTFRSFSASDLVFMLDPDLQRPDLLNFMKGTYASRSGSSLSPNASPSSDSGPNVAAIAGSIAGGAVACILLVAAVMLYSARRSRACRQEEQEAALRGLQAKQANQLEELSKRNPLAKLILDHLAGKEPAAATKLVLASGSTGASQACFSDVVGRGASASSSIGGGGGSGGGGAPAAAGNCFGTLGGDDECAGVNAAPTAVAVQLSPTTAATGDVGIPVNVNAFELAELFKHFTILGVANLPTNGGGADSGGMIDSRGSRGSRSMTKTITGGSGGGSGGGSGGSPGVGGGSGSGGGGALQPLSGFMALPKVVLQRWSRRSRARGMQSRERESTARQSGSGIAPGGGEGKGEACPQQQLQGSRGNSGAAVTAVAAAAGTCASAAAVAGTTTATVAAAADVAVRSSAAVRADTNGEQVGLNAPAAVATAAAAKGGGDDGSNMGKRRDGDGDRLGGNGGVREAGANAAAAPAAGSAANANANPATTSLPSQVHDQVAQSPVLEELLRLSLDLAGEIDDNQLIVTDVVASGGFGTVYRGTWHNLPVAVKIVLFSTASVNRRIALQEAALSKSISHPNIIATYAVDAKPMAVLGSRGVSSAGTAGGLRSGDSRSRSLAEIQEWRLYIIQEFADGGTLRRALDSGAFHDPHLGLPRMDMILDIAQGVAAALAHLHSKNIVHGDLNPKNVLLKVVPLPDSSSLPSAKSAAISGLLPGKLSGAAGTSSGYRSGTAGSLAAAAAAAASQYGHYPLAHRCGFAVKVADFGLSVKLENSHISGLRQGTPFYAAPELSQMGLFSRAADTYAFGVLLWELYWGRPIWVPDARAPGGYVQHADFPFLPQDCPREYAGLVGECLHLNHMQRPSFDDIATRLRSIVHSVRHLFMPAITLHSNRLSLSSVDNGSARQMAMMPHLEQQLQYALYHQQPLASDKPTQVQAQLKVQQQTAAAVLAGATGGGVAVGLGALPQAGLLHGARTRNSGTAAMVGSQQQQQQQHQHQQQSQQHQKVMQQQPQQQHQYHQQQQHQHQQQQQHQHQQQQYQHQQQQQHQHQQQQQHQQQQQQRHQGAGDGVSRGAAVAVYPPFPGTAGVAAARRSCEMDTNSAAVEHGCGHEVALARAAAAGSAAAAGMQLRGIDGGKADGGAVNRDGGNVQAVAAAEAVAKWKVKAATPAVSAQPLPSVVGHEAVDRYVQPDTTTPMGCIGGEGGMEVEVLGLEDRGKASSFDHPGTLPGFDGFAAGAGQVAGCGGRGGAGSSAAMSSSEGMSRMTPSSPRSRSRSRSVSVTEVTLRPGCAGCVTTTGDGGGGGGSGVEAAADTEEAGSKSSAASSDTITLQNSDDDDLWNTLTSPSGGSGTATAAGCSLGAAWGALTAAALAPGRGGGIAAMAAGPSAVVETRSVVLEAILEEPEEMEDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGR4</accession>
    <name>A0ABQ5SGR4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Metaxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_013675</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03054">
        <property type="entry name" value="GST_N_Metaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033468">
        <property type="entry name" value="Metaxin_GST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF41">
        <property type="entry name" value="FAILED AXON CONNECTIONS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17171">
        <property type="entry name" value="GST_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="10">
        <location>
            <begin position="42"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Metaxin glutathione S-transferase" evidence="11">
        <location>
            <begin position="190"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="265"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="266"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="38856" checksum="4444DCE8BA3DAD4C" modified="2025-10-08" version="1" fragment="single">TNDSAVVLYKRFRVAPVMSNQPICIIYKWSDFWGLPSLSPACIQAEAYLRLTGVEFAIEVCSTSSSSPTGQLPALEKGAYISPAASDEFASAAAVIAYAKKHIKDLDAHLSLCQRADQLAFKNLIETRLNVATNVTMWCESRGFNELKKAAYSEKLPFPLNQLVPWSKQREVRRRLAQADIDQVYPNALEVLDAIADRLRSTGGPFLFGTKPTTVDALLLGHLLFYRMSPAVAPVLQDKVQSQRVLCEYVDRLLVQNFSVPAVPRSALESSSSEGPSWTEAAKGQKKPTPTVVEPSPAEQQFRRHSYYWLLGAGTAIASYILLSGRYIQFATVMEQAGEDEADDEEEDVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGY2</accession>
    <name>A0ABQ5SGY2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FIST domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013702">
        <property type="entry name" value="FIST_domain_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08495">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00897">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FIST" evidence="2">
        <location>
            <begin position="60"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="466"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="669" mass="70014" checksum="0861FC369904E09E" modified="2025-10-08" version="1">MRHHLARGAIRAVATACATSNGSKARGFYFVSASYQGRSLLSAATELALTVREAMGPARPPHLITLFTTPYSEWGAGLADTPQYVRELLTYEGEQPPVIVGGVLRSVGAGSSHTAKEGAPCVALLAAHLPGVKLMPFHTTKSSLPTLPSDGWVEVVRATRAAAIRGTLDPGVPAPTVDAASLRPVSTDTMGPHQDPQGAAGMSGKAGPSVQQTDAENFEAMGIRAGVGTADGLPYISPRRAENMAAPDVGLGAIASQEVPNGNRGREARHRQQQELYRMDCRPSQQQQQQQPKQADAISGVADSRSQPQLAGARRSEHLGGSACSATSATASSVAALLLSAPHFTEVEDLLKRLGQVAPGMQVIGGVTAPGAWGDSESNWGAVWLNDQTYAQGVVGCVLQGPFKLDTIVSPGFRGAGPVMRVTQAQGQYVLELDGEPVQRPLQRAIAEALNSGEFASSLKIGLGDAAATSTTGSSSNGGCSDPQHRTSAMAAQYPGQQRPQTVFSSQPALHYAAATVGGGSKPSAQPPMQQPNKSQAQRQLVTRNWFFQQGPPRPFMTVQSSAPIANGTPIQVHLQNYPAAHRHMRDRLREYAERLPSGLAVAEGAVGSLALSCSALPFFEGPDVASCLPGTAFTGCKLEGEFCSTVDGQPSRLHSFTSCLAFLRAATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH14</accession>
    <name>A0ABQ5SH14_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="176" mass="20105" checksum="61A40FFA53BC2E6F" modified="2025-10-08" version="1">MFSMTYCHQKCLVDIPFANASRQAGSARRMRWSRHFTPASSLVEESDFVSSPTNLGQLERFVVQQQSTPGDNDLMSQRVDPRSFNERGLYIDGASIFASSVLLLVLMAFSTNRIFGLDRFFASAARSWKDEMRKRDELVIKTYNREGFTKAFMAYPLYPAAQLRLIGFSFRLTLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH82</accession>
    <name>A0ABQ5SH82_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013516</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="11852" checksum="3A3EE3E59B0CAFFD" modified="2025-10-08" version="1">MLALRTRTSRPRLPQGLSVGRQQALNHTAGGKHGHHYLLRWGAAVAVPQLWCGAAVKVALGRNPAAVMHAAAMRLPLRRRFVWMHQGCARDRACVDLLKTTARKRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHD6</accession>
    <name>A0ABQ5SHD6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribonucleases P/MRP subunit Pop8-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013945</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049128">
        <property type="entry name" value="Pop8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20976">
        <property type="entry name" value="Pop8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribonucleases P/MRP subunit Pop8-like" evidence="2">
        <location>
            <begin position="41"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="137" mass="14745" checksum="FA194615F8ECE01F" modified="2025-10-08" version="1">MMFNQVTNVPRQWTREVSCADVFMSLSVSFPGLGAEAATVTMTEQLFKSILQEALTDVYGLIGGAVNFVVLQVQGCSAIVRAHERDAHKLWAAAVFATKLGSAKVEVTVNRASPFLMSMSVDSRAWAASIVAGSFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHU3</accession>
    <name>A0ABQ5SHU3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013434</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="621" mass="63436" checksum="CBC986B66BA2D971" modified="2025-10-08" version="1" fragment="single">MLRSAKKATDGLGQQAHLPPICINRRYECRIHLDRKQCVPRAAEQRLESLLAATAVALLVEASLPSKLSYLCLAPKLANGAEVGATRVSMKLPLEYVSAPAIFLEPELSDAPGGCDRFIAASSGTDSSGLINLYLKNQAVSGAMATPYQMQIPQPLMVVSAVAEEGWRAGPPGRDLEPATRSQADGSGGSGRGSLAAADGGGGGSSPPSGVVESASLIVGGGLAGVAGVYFFASRGTRAAGDDASGSAPRTSGPASSGAAATQTAARAAVPTSEGRIEAPVQRGPYGAFDGISEERSYDGPPPRPGSASRRWNATQRQPAPSGASPAAAGSSPKPRPGPSPPQGGGRGPWPAASSPGVNAAPNAASPPYPDDAAEYTDAYPPPGPFYGPPPPWWREVYAEYDMYGGPYGPPPQYRGPAREEPPADADETVSGQASAPQDTYYYYHGAPGGYWGWDAARRRVSRPPYDGPYGGYDPAAAPPWPPAAPPSPPPSPQSRGFAGERASSSLGRNPWAVLEKEPSVPLDPRAATASKEPPLPSPYPTNARGTVAAADAQVSEPVAQKVSLTPASEKELSDLAGVDASLAQLQAQIDKLQGGGAVTSATSAAAANAAALTTEPDEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI02</accession>
    <name>A0ABQ5SI02_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="200" mass="21756" checksum="CCCC5A89391CC66E" modified="2025-10-08" version="1">MLLVRMPSFSLTSRNFVRLLTSCGMAKMLCHFSRASGAGSKHIQVGMELHDMVCGMCGAIVSTRSSLCCKMAYWGFKTPANFGMWLVITILLVTGAIWLIIGGLDTGGCNYCYSKFNKDLSPKHNGDSIDKGCHVLLFSFQLYEPKLALESDARGNMWGWCMLATGPVGELAAGSAMFLLGLLLLTYFCCAPRPSNVTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIA1</accession>
    <name>A0ABQ5SIA1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="220" mass="23201" checksum="4B7D15C77986A965" modified="2025-10-08" version="1">MSKHEGEVQQIGIETGNLSAPALLAATTETGFHPDQLATLIAQTTGIRTQLGKYLLSLLNLLKSTVVNTNMDNTAGPRVLPLPQAQGHDAIPEDPFGEAVALDQELGVTRPAPTATTRRSNRQRKARGGASSGVHGSGGAGPSSDPAKSRKCGGVPSSAAAQRSDPNAKRTRTDDSFMPKWNCNCSEFEGRKAANICLRCAESHPTKDCPLLPPFVPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIL0</accession>
    <name>A0ABQ5SIL0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="557" mass="57093" checksum="7B7B35778883ED20" modified="2025-10-08" version="1" fragment="single">MALPPVEAMAADVAAQQAWRRSTMPPHKLRGGSVMLYMQCYHDQLVRDMGRHTRRKHCSWRRPLAECFEPYTAEDYEDIFWKTPNPAARECGCGSGDGKSAGSTAICGILATTDPTAALEPRAMESKATVAVADSTVKAYANLVTAHSTSMPAVPEAQRSASWRQVLAGFGGSGGGPGSANCTVSRFHQPAGWPPSAPGPTAGGSRGGVGVLMLPFTASMSSLARLLPGAQLGQLSSTGPDRHRSATAQVKTQHAAATAGQCATAAIDSPTYGCTSSALYSMMRGSSCNTGPNCTSPAAVATQAPAPLMRSFLTPSAAPLAPVAAAVEGAGRSLQEGAGSGSAALRVGAGVGPHSQARMLEGLGLASEALTETIQQSTTSAMHLDEQDTLYSLDALPPALQTYRHTRVRVPTSGGAAAPPAAVVAAGLAAPELAGGPLRQPGKGYFGGMEDRDEGGASPFGTAVAAKYVGQLSLSASPSVWTARTRSIGGEQADARGSDASNAASSQRVAQKSLSLAPWYNGMQSPPRSPPRCSLHMEASTPSLLRGPGNMNMEIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJA0</accession>
    <name>A0ABQ5SJA0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PsbP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002683">
        <property type="entry name" value="PsbP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407:SF16">
        <property type="entry name" value="PSBP DOMAIN-CONTAINING PROTEIN 7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01789">
        <property type="entry name" value="PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PsbP C-terminal" evidence="1">
        <location>
            <begin position="102"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="250" mass="27311" checksum="ED0948AC3F233E85" modified="2025-10-08" version="1">MSFITKVRGPRALHSRSTSVTVVPRSRIVALAIRASLQSQNADEAAAAHLGRRQMLMSVAGLGLTMCTSASPVFAEGEYTTFLGYATPPTSYGGYGGNAKETPKYTFEYPTGWKEEVPNKVEKGTQGIDGRVVNPRSRDQRAFVITLARAGEDNKSFRLTDLDSTLSGFAGADYDLQDALSSATSRTTSSRELSGNTFYDYDIDSPEYHYLVTITVSQGKVFGVFVRSPSKSFAANEAKLRHIVDTFRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJE2</accession>
    <name>A0ABQ5SJE2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="123" mass="13209" checksum="0289665632DA8657" modified="2025-10-08" version="1" fragment="single">PVTSALCVCEHGRLDERFIQWEVRLFALYLRTLSGGAGEASSGGANALGGAWRSARGARGGPAGGRRVGHPWVGRGNDKQHPGAQELQLLFPVGGSASLRYYCVLKMTNAARMEYTNETEHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJG9</accession>
    <name>A0ABQ5SJG9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_014490</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041243">
        <property type="entry name" value="STI1/HOP_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF533">
        <property type="entry name" value="HSP70-HSP90 ORGANIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17830">
        <property type="entry name" value="STI1-HOP_DP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="3"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="71"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="7">
        <location>
            <begin position="137"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="382"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="421"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="455"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="7">
        <location>
            <begin position="525"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="576" mass="63911" checksum="1BD99494D76A27CC" modified="2025-10-08" version="1">MSADDLKAKGNAAFSAGNFEDAVKYFTEAIGVDPSNHVLYSNRSAAHASLKHYAAALSDAKKCVELRPDWAKGYSRLGAAYYGLTDFVEAAEAYEKGLGLDPSNEQLKSGLSEAQAALARPRNPFASPEVLRKLLTDPRGKALLEQPDFMRMFSDVQTNPSSINMYLQDPRMQLLLEIALGLKMSRPSDTDVPHPAANGGAAHSRHEEAKPAPAKAEAAKPAPEPEAGPDVDMTDEERDTAARKAEALREKEAGNEAYKKKDFEAAILHYDKALELYQGDMTFLTNRAAVFFEQGNYEKCIADCDAAVEKGRELRADFKLIGKALCRKGNALMKLGRLEDAISVYNKSLMEHRNADTLALLNKTEKVLKEKREREYIDMDLADVEREKGNTAFKEQRYPEAVQAYQEALKRGPPAVNPEAYKLYSNLAACYTKLGAYPEGIKAADKCIELKPDFAKGYSRKGTLQYFMKEYDKAIETYQKGLELEPDNTELQDGLQRAIEAISRFASGQASAEEVKERQARSLADPEIQGILKDPIMQQVLRDFQEDPRGAQKHLRTPEIMAKINKLVAAGIIQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJH7</accession>
    <name>A0ABQ5SJH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pseudouridine synthase I TruA alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014932</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02570">
        <property type="entry name" value="PseudoU_synth_EcTruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="5">
        <location>
            <begin position="294"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="79"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="552"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="576"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="586" mass="64128" checksum="72A1BA70607EFC26" modified="2025-10-08" version="1" fragment="single">KMLERKAALKFSSKWEPDLTATRCLQRQPRVNFVGFLNNNFTFIEARGSRSASSGDGAASAGGRCHRYRCFRRIVPSATSATPGHVSKGQRRGPESGRIANDDDDDGFPGTEDGVTWLATARRLKMAFTHACTSVPPAVEVVAYDGSEFAGFQFQPGKVRTVQAELERCAKRLFHGCSRMVGASRTDGGAHAYGQVVHFDVYGSRDSLESDVLYYNSFLPPDARVLGLSYAEDGFDSHFSSCGKTYLYKFAAGLPDPMQSKYRWWVYDRWCERSRGKPSRLSDVELDVGLMQQAANMLLGRHDFSAFMDNKRPAGLGSAKRKNPKLAERGIKPERTAAKNTRILSHLQLRESTSSVSGGQEVEMEVTGNGFLYRMVRMLAAGLVEVGHRRLTPAGLRELLQRADRSALPEAAPPHGLYLARVHYSGDAVFESRIQNLTQRMRESAGSRSRTCESAALMTDTASPWLSEQQGTGQWAPVGQVIAFDDMAEEVVRELRLTQGQKAAVLMRSAAMERQHRCAAGEAGDGLNPSVEAKGTSDGLGLKVLGGGLDVAASRPQQGQPAGQLAGGWEPGELIQLRDVDREDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJI0</accession>
    <name>A0ABQ5SJI0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="7">DMRL synthase</shortName>
            <ecNumber evidence="3 7">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_014232</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DMRL synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="3 7"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09209">
        <property type="entry name" value="Lumazine_synthase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="entry name" value="Lumazine/riboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058:SF0">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="225" mass="23639" checksum="11FE036DF425FD91" modified="2025-10-08" version="1">MASLHLQASLRNASVARRQQLVASRISFARPLIHTITFYNSHLQGSEAWDGRVRFIPSRNLCVTSTIYHGSVVAEPAAKFAIVVAKFNSLITKGLLEGALEAFESHGVEKENIDVAWVPGSFELPVVAKSMAKSGNYAGVICIGAIIRGATTHYDAVVSAATSGVLGASTDTGVPVIFGVLTTDDLEQALDRTGGKVGNKGGEAALTAIEMASLMSKLRLEGKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJL3</accession>
    <name>A0ABQ5SJL3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013981</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045237771" evidence="2">
        <location>
            <begin position="32"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="226" mass="24713" checksum="FC56EEB03E210245" modified="2025-10-08" version="1" fragment="single">MAGAASSGPQIGRSSLSLMASIFLFLNVVLGSTPETTGLLVSWQQVADRTTSKNTNRRMLLDGSSKDGKPLPPTWPSITHIYKPPTPKPPKPLPPSPRPLPRSNIPPPSPHHSPPSPQPRSRIPPPGSSPSPSVDSKKRPTVPAVTKPPPLIEKPPTPIRYRRPAVPARPNEPPREPPRTPRRRSMKPPPPPDAPPPEDYDYPPPKEDMPPRPPFPPPGEWPPFDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJT6</accession>
    <name>A0ABQ5SJT6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">eIF-4F 25 kDa subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">eIF-4F p26 subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">mRNA cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_014289</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI69622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="206" mass="23597" checksum="59C4A96112843953" modified="2025-10-08" version="1">MSDPKEEGREEGEVDSVEPDFSKKHPLENRWTLWFDNPNQKQTQKQYGQSLRSVYTFDTVEDFWCLYNNIRTPSQVNVGATYYLFKDGIEPKWEDVRNLKGGCWTAPVPNKGDSKKLLDAWWLNSVLACIGEQFADGDEINGIAVVIRARGDRIELWTRTASNEAAQMTIGKQLKQFLDISDSQKIGFSVFEEKIASGKAKDRYMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL41</accession>
    <name>A0ABQ5SL41_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DEAH-box nuclear pre-mRNA splicing factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015733</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF136">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX35-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="112"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="334"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="791" mass="85750" checksum="E50BF8FAE0A3B00F" modified="2025-10-08" version="1">MDQKASGFLRPGSQTSSAFGLKFERPSQRGNHRRGGDRQPSSTAYAGKRKHGAAAEQHPSSTSSDDDERCFEPGPPGMLPDGMRGPDLPPMQSIYRFGDPSLPVSRYRRQILYLVENHATTIIVGETGSGKTTQVPQFLFEAGWAENGFSIAITQPRRVAATTVAGRVAEELGCDLGTTVGYAVRFDNALTAGQTRIKYLTDGVLLREMLDDPLLSSYSVIIVDEAHERSLATDMLLGLLKKIQRRRSDLRLIISSATLEAAKLRDFFDGGTITAAAAKRRAAAAAAAAAAGGPLPPPPPDPNRTPAVVTVEGRTHPVQIHYLETPAPNYVHGAVEAAVSIHCEDLPGDILIFLTGQEEIQAAVGLLEEHARRLGSSGGYSLKLLPLPLYAGLPGAQQQGVFRSTPRGYRKVVAATNIAETSLTIEGVVYVVDCCFVKQRCYNPLSGLESLLVAPVSKASAAQRAGRAGRMRAGHCFRLCTEGDFANRLPEVTVPEMQRSNLVSMVLQLKALGIDNVMRFEWLAPPPAEALVRALEELHALNVLDEDARLTSDVGLALAALPLEPGLGAVLLASRNLGCCEEVLTLVALMGLHHVWVPAQGAVHALEEAKAKFATAEGDGVTLLNVHRAWRCNGRSAAWAQRHFLNQHALLRADDARDQLLGLMRRHGLLPADKPPPSCDNNMESVCRALASGRFMNAAVFERIEYNPLAPAGDKGTPVFRLVRYTAQRTNPIKLRIHNSSVLWRSMPSCVVYDTVQQADNGWYEMQGVTAVRPEWLIELAPHMFHKPGPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLE3</accession>
    <name>A0ABQ5SLE3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015672</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000095">
        <property type="protein sequence ID" value="GLI70797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="206" mass="21660" checksum="3BE245FF555EFD0A" modified="2025-10-08" version="1" fragment="single">VYVRSSSAAADVTPAGVGAGTSAAQQQQSQEEVFIADGSAGTTTSGAGAGARVGSGPGAMLRYYFHIGSVDSFERKLDEAQRELGIPPSQMVPVKYVDEVSLVNVLLEVAPTLLLMGATYWLISRQMRQMTGMGGLGRGGLGGRNGRGGMGGAGGFFGMSKANVSFMDKSKERIVFRDVAGCDEAKTEIMEFVDFLKNPKKYIDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLS0</accession>
    <name>A0ABQ5SLS0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_016026</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="924"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="176"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="398"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="698"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="742"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1513"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="409"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="742"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1516"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="951"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1537" mass="156976" checksum="ECFB6B15BAC4A55F" modified="2025-10-08" version="1">MHLMPCFTGCFGIDSKANVNIEFDGLQEQRACAGGARPITGGRRPDGCRCAEQFDCAGLVPLLTGESHGEWWDILQGACRALTMRLPADHASIFVVAGAGVAALATSGVPLFGLQLGKLLLPGATVSNTETSDEESSSPLVRQSELGVCVLAAALQDQTASVPLVFRSVKLNNLEQQQQQQQQEQRQRQQEEAGHGGATGHSHEAARYLAKGTCPRCHNPLQTKTRQQDGASDDGGPHQRHFVAVGIPGGKSLAGMYILSATGPSVPAAWQAEGLGLVAALLAPLLRPPQVPLVRRALAELHTACTINDLVHSINAAAAELVVAVTRVAMQPRLALLRNDGTAAAVFAVSEGGANSAMCRRRSRDVVLVDAGGSGFSNCKSPDFFMLGHPSFYSPEQDSLGTAVTASGAPGGGTGGGTGGGTGGGTGGGTGGGTGGGTGGGTGGGTGGGTGGRIFGKSGDDAALQRLRGAPSGVTVPSGSSCHGQSLPLHHTLLAEALTRGSGLCIADCNAYVQDSKAFPRDLMISLRGGGSSAPPQSLALATSCHSGRPLLALYATYHSILPQALLQTVVQELGQLLRALTPTVAALLLPEGPISEEWSLLHDDLLGPGCVGLTSGHSNKARFNSSIMPPSAATTAAAVAAAATAAATVEATLAATARATVTAATCPPPMLVSPVPGCHGGGGATAAATVAGTDGCGGGSTNGTPTYSPRSSRVDPQPRGHAAGTALGVVAQIGAFFRRTGTTAESGRQQNDTPGASPNIARCATAPGGGGDCGDRVTSPGVTAVTAAAVVLAGGVNVPVGTDAVAAAGTDGIEGRSDSTTTASKRLAAFVSGVSGVGAAVDVGGAGPQPGGQSLRSGTSRKALARCRTGRTSFSLEVVGSPRGGVRLAPLITTLHDRLKSAQAEQLTASSRAASRMQDLESLRFLERIGKGGYGSVYRGLYHGSEVAIKVVEDPGLGNTAAAGRPLTTAASVSLLAKHIHDAIELVASVSMSHPNIVQLITYFVDVRAQHCHHQDRKAATLCARSEGNDPATDAADGAGAATGASESLHLVRMTSSGMDSSTSSLTADPGAKEAIVLVMEYCDRGSLKEAIQDGSFLQRETLTHVISLHKHHQQGGQQQQQQQQQQVQQMAALARGSMGLFNMKAVYSTLLEVALALRHMHGLLMVHCDLKPQNVLLKSSPRDPRGFTAKLSDFGLAKMMEHDEEGQLVIDDAIQSGTITHLPPEALIGGRQLGPTVDIYAFGIVMFQMLCGMTVYRGLDVKQIIRGVVRQGLRPAVPAWVPQAYRDLAQRCWHEQPSCRPTSLQLVSELEALLEACDGQQGTLNRHGFAGTGGSVGRMRLGTGGCVSDATRQRRLPPRCATDTAIQGRRFPQTAAQGSPMPLTIAVAPVNAVAIAAGSCGGGGGGGYYAATNDVAPLPPQLTATMLRHPPVGVYHPPQSLYQPHSTGTGSRSPAAGPGVVDLGLGPGTVASSVYPVGIGGRVAALHPAPLPLPAAAVAVLNQTSLYTRTLPAATPPPAPPALPQPTPHPVLLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMS6</accession>
    <name>A0ABQ5SMS6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DWNN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="20237" checksum="6A42E278F5A35F7B" modified="2025-10-08" version="1">MIAISSIASFRSVRLRADTYEQTRTRGTTYDALVKFARGTEVRFLAGQDDPKPVPRANAVQAQDPTDEGVTNDHDLGVVRPTTAATVMCTERRRCARGGSGTGARGSGGAGPSSGPDKSKKRGGAPSAAAVQNSHPRAKRMRTEDPFLPKWDCKRSELERRKTANVCWRCTADHLTKDCPLLPPLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNI1</accession>
    <name>A0ABQ5SNI1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008507">
        <property type="entry name" value="DUF789"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32010:SF18">
        <property type="entry name" value="DUF789 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32010">
        <property type="entry name" value="PHOTOSYSTEM II STABILITY/ASSEMBLY FACTOR HCF136, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05623">
        <property type="entry name" value="DUF789"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="693"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="795"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="972"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1400"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1626"/>
            <end position="1660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="478"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="609"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="697"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="708"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="984"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1092"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1141"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1159"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1192"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1432"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1453"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1471"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1497"/>
            <end position="1522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1714" mass="178198" checksum="E0E880C3E5C28BFA" modified="2025-10-08" version="1">MASYPKRARGLVTEVLCSPGEQETLRAVSAVPPEQQRLVFGPSAPLVAAYSGQDPLFAELQATQVIIFQDTRALGRLVNPRVLVDSPRELAPVSLVSLIYDYWQPASVDRKQEPVQHLRIPLDSRYCNTSVFCIRAIVTGWPGGAVALPGRHEWSGSKTAAAVSQHKQRLNYIRTQQRLFTEGEGKDSPNRHLLARSDAEYTIDTGPEWEASSLSLTFNTGAELPWLQRIILKLLVIRKAPVDDKGGPVGETSAKATVSSSSVGTSACPLDMASASPQPVPQGHSTRGNLKPTDDRLPQAQQAETGTSYSSPVGCSASDTEAECIGANTPVNDTPTKSNARLSPSVALPANSGEVDDCCRRGASQPTWPTGGGGESHDSSDSEDECAAHRQLGNGQEQGGMATDGGSGLDSGGDRGDSGREREEGQEGPGASPNSDQKHKGKGYSEVSNAMINGRESQQSIILCSSHSDREAAASLQDASSRSTTMDTADASSDAADEPHAGVAESKPTVRLIQNCSGLARGQLEEGVFQLGVDQVLSSEGQYICMEGDGIFRAQGGAAADAPGCANGCCGRVNSAPRDAGDENPRLASETMPSAVDSNKAEDFAVADASSLRAGSKSSSASRRQSDETGATDRLGIASSQPGEELGNLVGSSPGLRKAMAAAASDGVLVMNACAGSQPLPPPQAAAFNASLASTQLPPPPPPPPPSSHHQQQQRSYSNGAIWPQTHAKGSSHGSEQPQQMLSQPHASSTSHIPNLSQVSHHGAHTHSLQNGKLRNGEVPGDLTQWAQQLLPNLGMVVPGQGGSGSATPSPGPQPLHPNPQSAGQSQGLTAANVQNILLAFQSGTLQMPHQGLPLPSQHLQSGMGVYGLSLHHQQLLAHHTALLQQQFLQNILHLQQHAAHQAPQQVQQQISHEPRQQLGTQGLSHMHLQSMETQYGAASTGDAPTAFASSSPTLTITTHTSTVPCTSTESAAETCASPAEGSIQQQPQGPIPIAAHPLHPAPIAAPTPAIAQHQSSGAQSSDAAGQSGWDSGSASSARSEPSTAVAPPLVERPIGPQHRVQSQGNILASGHGSVTGSQLGQRDGGAGGGRSSYRGRTSFSGVAGPSTGPPAGSASSTGAAGQPSGMAPGGGAYGSRSYGGPMGSSRHGGGGFRGGRYEGSSADSSRPSSRGPHPHGVGGSGHMRQPHSADPVPPPEASPWPDPKGLCDLDRFISQIEPVIAIDHAKPPQQALQELKLRDLWNFYFEPSLYGREVFTLGGHRGASNSYFVPYLSAIQIFTRALPTDVGGTNRLYVSEDNQGWPKHMHLKFEYFEQELPFNRLPLYDQIELLSSTMVGGKNAGSGPGCAGGTAGVPPASGSRPAPLAQPAFGHAAVAAPSVAGVATSSAALASGGKEAATDVQDATEKAAPQAAHNIDRSVDAEASEVDDPAGKMGAEAAAAAELPGAEGASAVTTGRSSQAESIAECMTAGRNTSTPTASQPNAGPISTHQASAVQPAHAGTARPPAATAAAAAWSSEAENSGSSGGTGLLYDMRLLDVHPASWFAVAWYPVYRIPDAPLYARFLTFHSFAPPVESMRNVHERLAMGQVAPCNLFPVPVVGLNWYNMQGERWMEPLDESTVVRTHDGPAPQPPIAASGPTASVNSPRGPPLHQRGAGGGGRVPGTDVWYQQWLHNFQVTADRLARGVGLKVLGQHGAEEVRLRVPDYEFFRARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNN9</accession>
    <name>A0ABQ5SNN9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="106" mass="10900" checksum="80705385DEDD1197" modified="2025-10-08" version="1" fragment="single">IVGNDDRRDADGDTGAADFAALPAVRGSPTGGLVSAGDPLVSPSRFSSNRGRRGGGRSFRSRPPESAQGQMENHSSTVLGEGSQTSLAKTMGTPSDMYVAGHKPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNU2</accession>
    <name>A0ABQ5SNU2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SURF1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_016931</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probably involved in the biogenesis of the COX complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SURF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06662">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002994">
        <property type="entry name" value="Surf1/Shy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045214">
        <property type="entry name" value="Surf1/Surf4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427:SF2">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02104">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50895">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="451" mass="48342" checksum="CB481D69EF479663" modified="2025-10-08" version="1" fragment="single">CILFNSFGCDSALPRPRPERIEHLPSTTWRNPPMRRLAPRGFDTLRAFARSTQVGTGSQNAAATGFRSWLSSNAATSPPTATGGAGRAGASSESGGSTWSPLSAVMFIPSLVCGCLAYWQFERMKWKEELIGRRRGIAEAEAEEVFSRDKPLLEYDKVKVQGRFLPEYSLFVGPRPRSLPGSGIQPGYIVITPMISTDRKGVVLVNRGWVPAAWKEEAEARAAAKLQEARAANAKEEPPATATTATTAATATTRSWWSWLGFGSSSRGSQTSSAESQPTVAVKVVPEPDLVVGVIQLDEKPSNFMPENKPESDEFHFIQREAMTRVLGLPSDTPLVMAISTDPAAVQAVHKRSPLNEARSAASAAAAAAATAGADIGDAGPTYPAPKHVSDLLRFSTMPGDHLNYALIWATLCIALAFMARTAITKPLRGPRIVEAPGNSARDAWKASQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQQ2</accession>
    <name>A0ABQ5SQQ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_017141</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M43B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000121">
        <property type="protein sequence ID" value="GLI71798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47466">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47466:SF1">
        <property type="entry name" value="METALLOPROTEASE MEP1 (AFU_ORTHOLOGUE AFUA_1G07730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14295">
        <property type="entry name" value="PAN_4"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046422575" description="Apple domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="4">
        <location>
            <begin position="136"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="4">
        <location>
            <begin position="357"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="4">
        <location>
            <begin position="588"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="4">
        <location>
            <begin position="809"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="436"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="669"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="889"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="438"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="890"/>
            <end position="906"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1048" mass="113809" checksum="5D900F7E949B77B1" modified="2025-10-08" version="1">MLMSFKMLAIFVFVLPFAAGVSGTARRDLSSSTGYTTAVQAVTIPPVAPALPWGCYAKIDFLGKDIRIINQTVDYLACRQECYNEPMCTFSVRTLDRKCYLKYSFLNGTAGSNNYSPNYVDVLCFVRPNYGGQYQCAADYDVKGDHVGDIAKACPSFATNISLQSCSNACSQDSRCEFFVHTDGNYCYLKLNPLTGVCGPTNTSTYVDKVCFRASGPPSPPPTSPPPPSPPPPSPKPPSPPPPQLPLPRPQPPPKPLLASPPPPSPPVAPALPWGCYAKIDFLGKDIRIINQTVDYLACRQECYNEPMCTFSVRTLDRKCYLKYSFLNGTAGSNNYSPNYVDVLCFVRPNYGGQYQCAADYDVKGDHVGDIAKACPSFATNISLQSCSNACSQDSRCEFFVHTDGNYCYLKLNPLTGVCGPTNTSTYVDKVCFRASGPPSPPPTSPPPPSPPPPSPKPPSPPPPSPKPPSPPPPQLPLPRPQPPPKPLLASPPPPSPPVAPALPWGCYAKIDFLGKDIRIINQTVDYLACRQECYNEPMCTFSVRTLDRKCYLKYSFLNGTAGSNNYSPNYVDVLCFVRPNYGGQYQCAADYDVKGDHVGDIAKACPSFATNISLQSCSNACSQDSRCEFFVHKDGNYCYLKLNPLTGVCGPTNTSTYVDKVCFRASGPPSPPPTSPPPPSPPPPSPKPPSPPPPQLPLPRPQPPPKPLLASPPPPSPPVAPALPWGCYAKIDFLGKDIRIINQTVDYLACRQECYNEPMCTFSVRTLDRKCYLKYSFLNGTAGSNNYSPNYVDVLCFVRPNYGGQYQCAADYDVKGDHVGDIAKACPSFATNISLQSCSNACSQDSRCEFFVHTDGNYCYLKLNPLTGVCGPTNTSTYVDKVCFRASGPPSPPPPSPPPPSPSLPSPDAGSFCLRKAYNRKEGYYISPPCKSGYDEVYGMCIPKCADGYSLSDLFFCFGEVPSSYPVMCNSAVWATSQAGCDKINKVISELRKKYGYSSDECAASIYGNCNSWSTDQIIAFCGHYMLGFYEVYICDSVPLPAISVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLW7</accession>
    <name>A0ABQ5RLW7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000336</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007133">
        <property type="entry name" value="RNA_pol_II-assoc_Paf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188:SF12">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03985">
        <property type="entry name" value="Paf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="491"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="526"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI58605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="549" mass="60012" checksum="870B372CEEC74ECB" modified="2025-10-08" version="1">MLAGLPGPSAYGAAIPSAVGPSGPPPPPPSGPGTAGYGPASYRPPGQPPPKPSGSYGPQQQQNGFRKPDPAPGFRSVSPGGKPATGPSQSQHRQQQQPHNASHASSSIIPMAVENRLRRDTPFLAHIRFKNDLPEIPSDPKMLVSQIQPEVLSRFALTAIERQARRDLLMPPNIVISALDVQRYQVPDQPGPIDPADAALLKDDSRPLTVGGSSLASVGDGRHKSFAARTKGMDVDKCSWLMRTTYISSSDNRGVQRQGLPEKQVLQARAKERRGANDADDRELNDVAAQARAIEASFEAARALPMHSRNPSLKPVEVLPVLPDMGSWPEKLLLTTFHDSDPAEELVAAVGAESIGALPAAKRSKLLVGHYLLKGFTHHISKAGQDRELKIMALLVREDIDQMLARTAQQAELHAAAAADGNEQGAGVTGGGMQPEDLEGSYQWIKDYNYEYKRDSVHYAIRLDKGSARFYRMQGKLELRSWRDEEKLARDRSRKRVMGRAGSEEPAEEDVDGSDQVQRPSKIRVTTRDLTEREQQERESKRRELMPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMG9</accession>
    <name>A0ABQ5RMG9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Lactoylglutathione lyase</fullName>
            <ecNumber evidence="4 10">4.4.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glyoxalase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_000526</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>(R)-S-lactoylglutathione = methylglyoxal + glutathione</text>
            <dbReference type="Rhea" id="RHEA:19069"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:57474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="4.4.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glyoxalase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.5" evidence="4 10"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07233">
        <property type="entry name" value="GlxI_Zn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004361">
        <property type="entry name" value="Glyoxalase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00068">
        <property type="entry name" value="glyox_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374:SF30">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE GLYOXALASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00935">
        <property type="entry name" value="GLYOXALASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="VOC" evidence="11">
        <location>
            <begin position="64"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI58773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="222" mass="25147" checksum="9650A865718B2EB1" modified="2025-10-08" version="1">MPLASLCNTSRAAAARAWAPRYWARTSAPFRKHPCRPQAMASLAELQSTPGVCANPDPATQGFVFQQTMFRIRDPAKSLDFYTRVLGMRLLAKLDFPDMKFSLYFLGYEDLKDIPEDAGDRTVWMFRQKACLELTHNWGTESDPNFAGYHNGNSDPRGFGHIGFSVPDVYAACKRFEELGVEFQKKPDDGKMKGLAFVRDPDGYWIEILNAENSRQFATWGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMK2</accession>
    <name>A0ABQ5RMK2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DnaJ homolog subfamily C member 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DnaJ homolog subfamily C member 16</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Endoplasmic reticulum DNA J domain-containing protein 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_000567</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays an important role in regulating the size of autophagosomes during the formation process.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019345">
        <property type="entry name" value="ARMET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052460">
        <property type="entry name" value="ER_disulfide_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44340">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44340:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10208">
        <property type="entry name" value="ARMET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046103309" description="DnaJ homolog subfamily C member 10" evidence="7">
        <location>
            <begin position="23"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="28"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="177"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI58801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="475" mass="50214" checksum="4F716B7DAAF24D82" modified="2025-10-08" version="1">MALRLFLATTFLGLLLARHAIAARGGRDFYSILGVARDADEATIKKAYRKQALKWHPDRNPDNKEKAEERFRDIAAAYEALSDPEKRRIYDQMGEEGLKGGGGPGGHPGGGHPGGFNVHFGGGMDPREMFKMFFGGAEGASAGSGMGGMGGARMQFNMGGMGGGMGGGMGGGMGGGMGGGPPMYDSDPYVQQLTGNSIPPDNNWVWIVEFYAPWCGHCKQLAPHWSKAAASLKGVVRVGAVNCDEHQNVCAGQGVKGYPTIKAFVPSSGREDGGRAYGGERSAKAIADWALSLVPNRVAQLADHKSVDKLLTQCGGKGKDRAAWGLCVVLINNKAETPALYRALSTAYSGKVAFGELRASAKAAATIATKLGVNLEAERDGGKLPLLLSVCNGEVALAERYGGQLKSEALVRHLDLYAAGKKCAKMVRLDVNTDFSGFSAGRLKELVKDKGLECVGCTEKSDFVRRLQEYVTSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMS9</accession>
    <name>A0ABQ5RMS9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_000631</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF0">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="10">
        <location>
            <begin position="21"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="11">
        <location>
            <begin position="332"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="697"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI58865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="716" mass="77981" checksum="B9AC644AA731F097" modified="2025-10-08" version="1">MDGTTTVRLERAPSMPSRFFPFPTNSWWSTWTHVSQYTLKMGEELVAMHPWRVKVLPLGIELIPPGTQFVSQWNFIISSGSPYLRVTAAEGLGSRNVSAGNEMGVTFEWNRADGAVGSMQSIMLQGCPFVNVRYNGLTPVVTQLPNAALIDELGTFTGRTFKVMNNAGMRFKYYFSSQVTATITASSLRLASPFTGVMRIALLASDYLPQLSAASAEQLYDANVDIYPTAATTSFGYKSASGSGSSETGILRISFNTASMSGSYQGQLLMLSMPHHRTHLIYPNIADASGVTIADLRGSLTPVLGSDWYLAYQLPNIAWNAPNGVSNATMRSAVIAALKTDASSWQGRAPAADAYFGASDLAALGRMAVIADELSAAATTTATDANDLRAVASSLRTLLRNHVSARIVTNPGVEASLVYDTTWGGLILYRDASTHTSNYFGNSEYNDHHYHYGYLLYAAAALAKGDPTWLATRKDSLLALVRDYANPRRDDPWFPLARMMDWWGGHSWASGILAMGDSKNQESTSEAVNSYYAVSLLGRVLGDPDLTRWGQLLTAIEVSGAQHYWQIPSSSSVYPAPFRDNKVVGVLWNGKVDYATWFGSNPSQIHGIQCIPFTPISEVLLQQGWVAESYPIASSRPDPQLPPCWTQFPALARAVVDPDAAWSSIISISSPSAFYGAWAAHPKSVELYWIASRKTSGSQTSASRRLLQQPSRLPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNE0</accession>
    <name>A0ABQ5RNE0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small Arf-related GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_000767</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00878">
        <property type="entry name" value="Arf_Arl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044612">
        <property type="entry name" value="ARL2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45697">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045088216" description="Small Arf-related GTPase" evidence="4">
        <location>
            <begin position="20"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="19491" checksum="B9F7A116C374DBEC" modified="2025-10-08" version="1">MGFLRTLLIRLGLVKTKVGLLIVGLSNSGKSTVVARLTKEPVEEVVPTVGFAMESVKMEGLSVTIFDMSGNNRYHELWKLYYKDTTAVIFVLDAADPARFPEAETVLQELFKADLLKKVPILFFCNKMDLETAARPAEVAAALHLPSASSRGFQLQSCSALRGDGIKEGMQWLLDQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP36</accession>
    <name>A0ABQ5RP36_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Copper transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_001153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045554408" description="Copper transporter" evidence="9">
        <location>
            <begin position="21"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="643"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="669"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="201"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="201"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="286"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI59300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="758" mass="80240" checksum="4ED61D2F00650DF8" modified="2025-10-08" version="1">MSRLHFIVLWWLSHIFLTVSQLTCPPPPVILHSTLVSVCSQEPARSAIGCSMYRLCKNETLDPPIFSALCDTWRVASSLCSDDGGIADSIGLCKQLQASYLDETQFRSCLVPYNMTSTSEIRSLHLGACGEMARLADMLGCLSCTKESCPDPLVSYSEGCYSMDMGHCGPWLDFCLSDGSPYGAATAVLCISPDFMMPGVAPPRPPSPPPDPPTPSPSPPPSPWPRPPSPRPSPPPSPWPRPPSPRPSLPPSPQPPLPPSPIPLSRPQPPQQPSPPPAAPGVILSPPSPNPPSPELPNSPQPSLPSSSPSPPSPLPPSPPLPSPLPSNPELPGPFLPSLLNPSPPPPRPQPYSSLMLDNSFQIVQTPSCITNSSLPQCASYEYPSSSITADIMSLCTPMPYMPGCTIRSACQAGNVTGDFCRPMTLLATICLDMPSMSGCKSYVALCKNTSVVQQCRSFPAIPGLPSTNEARKAVMAMCSSMDMVECETCTQLSCNDYIGAMSSMCTEMPYMIKCDVYATWCKASMAWVDESGGSSDFSHYCTGAGAYGAGSSSSDIPSMLMFFHQRTREILLFRTWLPKTTGQAVGSFVAIAAMSFTSVALRTAQFILVAAVAAGRLAPLAPSPQPSVHWWLPSGGQALQNLVSSIITLLFTALDLFAMLIAMTFNGAYFTAVVLGYGLGTLLLGHLRENYQRHVGAEANGVSDMEICCGVVIPPPSTKQASRGGTSFTGIAMAVTNGSGGGNQLNAKQVDSNGGHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPR7</accession>
    <name>A0ABQ5RPR7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001407</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006968">
        <property type="entry name" value="RUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055412">
        <property type="entry name" value="UVB_sens_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054549">
        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770:SF22">
        <property type="entry name" value="PROTEIN ROOT UVB SENSITIVE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770">
        <property type="entry name" value="RUS1 FAMILY PROTEIN C16ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24160">
        <property type="entry name" value="UVB_sens_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04884">
        <property type="entry name" value="UVB_sens_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="487"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Protein root UVB sensitive/RUS" evidence="4">
        <location>
            <begin position="295"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Root UVB sensitive protein C-terminal" evidence="5">
        <location>
            <begin position="672"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="119"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="578"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="799" mass="84863" checksum="37D5A5C1382C95EC" modified="2025-10-08" version="1">MRQLASNRFSLAKLARNGRKQPEDASAVLSCPSARFLGKARGNSVAPVAAQESTSSQVSLTHSRYPANTTPNDAFSESTWRGFAAQAGVVAAANTAGPVAHTSVPLQSSNTFPVVPETAFGSMPPDTASLSDSGPGRPPSIGVSGATVDPHSIPPGNPGDSKHHTASCRDPPAAEATRATTAATAATTEIYTVVATAPAAASVAAAINVGHRAYNEAKDASFSGRVVQHDGSHAVHLLVSGTTSRDYNLRMVRCHARDELRLPSTTGSVVGSGGGSLCTTAARTEDVPFGTFLYRQVRDTITATFLPAGYPDTTGQNYVRFMTWQGVNNMAMTANTVLASTFMLYAVGLGAGSIPTAGALNWVLKDGMGQLGTLLFGKTIAHNFDVHSKTWFFLSAVMLQAAAALELLTVLVPEHFLLMGSLANTLKGLAWMAAGSTRSVFHLSFARDNNIADVTAKGTSQYIFASLVGTAGGAVMCAAVGQSGAAAACCFGLLAAATIGSAYMAVQVIPLSTLNATRLQLLVDAFLASISPPDVHDRLRRHHHGRQTYVGNEIRDDGGDVDGAAYRGAAGAAAEADDINDGARGSGSGGVSSRAAGGFGGRDRDRDHGSAMRRCPRPRAYRTVDRCYIGPSTHEYEMMYDDSMDPQVPTPLELSPLDPPLPYFSRAAYRLSPDIMVGSRLEHIVDGNANLLVMLLTTYKYAHFMVVPRREALHVVLHERADPRDMVQAYLQACILRRRLRSDQPLPDPDEQIPELRLELQESLAAAERLTTIFMSTIEANGWTVAKVVVEAQRRRARW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ13</accession>
    <name>A0ABQ5RQ13_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_001390</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May play a role in vesicular transport from endoplasmic reticulum to Golgi.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Golgi apparatus</location>
            <location evidence="1 8">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14942">
        <property type="entry name" value="TRAPPC3_bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016721">
        <property type="entry name" value="Bet3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13048">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018293">
        <property type="entry name" value="TRAPP_I_complex_Bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="185" mass="21077" checksum="05DC481C81BE8781" modified="2025-10-08" version="1">MLRGGPAGARNAPSLEQINAEVFTLTYGSIVRQLISDFEDIEEVNKQLEQMGYNMGIRLVDEFLAKAKITRCSSFRDTADVVAKQALPMFLNVSANVANWNPDQTECSLILTDNPLADFVELPEEYRELKYCNVLAGVVRGALEMVNMEVECRWVSDMLKGDDCYELRIKLNEHRDERFPFKDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQS6</accession>
    <name>A0ABQ5RQS6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_001806</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="216"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="328"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="497"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="538"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI59885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="580" mass="59110" checksum="606E6B70D2CAF51D" modified="2025-10-08" version="1" fragment="single">MKRARVEGSCPHINEEFSAVDYGNIILQHSSRNSPGACHACCACGQIVSNRYGGAPDGDPILGTILRGCACGSVMLVDVVRCELYCCSCHDYVYHSAFDEGRQLSHVARRFIDRGLRPGLPHHDPGPNVLVKRSRVGGSRVAAVLAALHSDSDTGTQQLLPLLRDMTTSATERKSERPGPFAVQEEVDTTRHVYGTRTVPVGCPDVTSDGFPAGLRGLNNLGNTCFMNSVLQVFLHSPLMRNYFLGQGHSQSSCARLGKSPGAPCLSCELDAVFAAAYSGSRAPLSPTDFLYSWWTFADSLAGYRQQDAHEFYLSALSGLTAAVITEPGGAATQPPANTSPTSPPAAPPAALAAARSTNSGWAGASTWPPGPNASVGALSAVAAAPYSRPLEGVGSPGLSVAGGTVSQLGDCPGAAAGVAGGVGPGGVGGHGGVGGHGGSGGGSCSPDLGLNLVDAVFGGVLRSDVTCTVCGHTSTAYDPFLDLSLDVVAMVPELPPPPYGTLGRPPQSESTPASSPAYAPKRIPLDEDETDMEGGSREAAAAVARPSASTCGAHSSLSALSPPSPSPSPSGITGVALAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQT5</accession>
    <name>A0ABQ5RQT5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001503</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="528"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="600"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1017"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1161"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1289"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1666"/>
            <end position="1729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1877"/>
            <end position="1902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2227"/>
            <end position="2246"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1083"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="664"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="693"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1026"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1303"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1324"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1697"/>
            <end position="1710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1711"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1882"/>
            <end position="1897"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="2366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2366" mass="245435" checksum="E5EAE557DD5EB51B" modified="2025-10-08" version="1" fragment="single">MASHATEMDAEAHEYRHANLRQMNPGARKSVNWARRHPPASDADGFPVYGDSGSGDGEWREGAISPLEEFDGIHYSAASSTTGGGYVSRYSSRYGGLSRSVSATTGAGSSRASEVWESGTVPYERSTSMLGRAAVRISSAGTSGAANTSPGYTFGGGSLLLPGAGGLTSRPGTSDPYGGAGGGGTGTPPISPSKVGLSARQRAMLDASQVGTFRFARIKRATTPLSVASGGGGASIANLDTEDGGAGAQAPLVLPGVGGAGGGICRTPSMRISSNAAIGPSPFQSPMAPYRRSTAPLPAANHRPSQGGNARLAGDGNGNATGGMSPPSTAALASAIMQELNARQSDHDAALGGSATNGRLDDMSLVAESVPVLPASAQRTEVLANFVRPGRGALVQPGLQKMPPPPENEVSEAEALLGSAAGGTAGGGGGGAGSGADAFDPAAASSALLDAELAPLLREVAEINVRPAVRAALSKLDDRAIKQLFAATSRRVEHLPCTPVSLSSGGGLLLPLPGPKGVAGYRVNMKGQVHPTKNTGRHFSRDAGGRGTGTSASDATAADAMLLQQLLEHDLETLPPKIRALLLAHAPAETLDILEEIERSASPHGSGGGSPTPGLAPGSVSRRGPRLSISPRRRSANGELPLHGHTHVHRGGLHGSPTHSSHGSLSQQHPPQLHPQSTSHSRSGGPAHPGVGASSSNHHSQHPGSESRSNQHHIHHELQHRQHHPSTMGTAAAAGAPYPDPRTPALSHLGTGQHSQRYPAGTRSTPEEGLLSPSPVPDRVSGDLAAVAPDRASPRQDLRVKLPRQTDNPVGAGAPGELLSPYASAAAGGGGGGGGGGGGGGGASGCASAIRSASNTAAGCSRDDEVEDPLEDRRWRLGQLMAEVEMQQIPDAMLRNSFVYHQAGTATDGAAALAPYTDSMIGGTAQALLASLDHRVAPADSTTTFRQVVTAAIAKSGPLPAIAHGATNTTINGIAGATAGVQDAAVARSDTDMSMGGSAVTEAAAVASAKRKIDISSWAGPMGGAEDSEEEAEDEEDRMVTDQELGLLTLDQTLAQLPTSASPALQTSDALVARLEYTVKRRAALAERRALREEEARRRAEEEAARYIEVPLGTEDPNDDLVGPLRSGLRGAVLRMRRHGETGRENMLKQSGFFCWPSSRLRQDQQSAPTQGAPVREMAPVPTEPSTDPASRHLRFLQRHALTLGVNAQPRFGSGASATGDGTPLSTASPASAYHSMPYRTAPTHYTIPYYSLHLTNNRHGHYLNYLHELQKEGSSSTNSMVAVAAGAGAGSGAKSGSGTSVWNASGSRSISPNPERNRVAWNLQPSSPHTTQGRELSNTASGADKSAAAAAAAAAAAAAAVDLRYSNDEQRQRLEPVLDAWEVVSSGDTELAALGQFKEALQPASWRPTPADMKPFTPLEGEPIRITEQQVSRRLVQAAETGSSILDLDFGPRLTLGDFGVVYAAIKPLPHDAVKGIRAVSGVVAKSAVEGLTALVRARPSIEELELRGNPALGATAMSRLAPLLHPSMRCRLRRLDLSGCPLGDAGFSALAGNLAENRHLQDLLLSGCGLEDAALAVLYQSLALNGSIKLLDLSQNRFSETGMTVLCKDLEDNRSLTRLDLSHCSLPDAAMPPLSRVLVLHPALTSLDLSHNCLGWKGCRALAEGLQPQPQPPDPLQTTHHHHHDQDAHSHGNAQHPFSFSFPQSRSPSPHHHHYHSNLQHHHYHGPPRPVLHELALDGNPLGQGGVLALMRLLQVNSTIKTLSLSNVTLTALPGEPLELDPNHPNGHYSLDLADPEQRKGATTLLSLWHASGGSSWLSANLNNAPVHLPRAAAGCITPSGTGSWPDWVRPHLRWPEAMPTEGTLVVSVRNNASLEPSLLTPPPPPAPPPPPTAAEPPLAGADPPAALVVPAAPVVVPPVTLSPALLSVLIQAIDSPAASEVWKTQLLTLATKVAYVTSEQAGHLLGHLRYRAERADAAATLLSSTPDLHRAPWALRTFAKFGPDDIAELLQELSLAPLLPLRTRVVNGWDSCCSGHIRLDLAQQSHRLLAILLVQIAQRDSGARAWATPQAAAPEEDLYGALTARLGDATSCMRDITFSGAPLGHSVIYLSSANIPTRGVIDTHFVNVRPRTHVQYPGLGMGKERRQAPSILTGKSAAGIGAVTTSNSGGAATPSAVMAAATASGSGGSFASPASVATAAAAATIATTGGGGEAAATVTAAVDSAGTRGSTPSTRPTPTTAAAATQPLVPTVSAGVVMMESMRMAASALSSSVALNAVVTKKNWWQPCVTAWEGVFMEALLRLGLSHSHSALVGWRHAANMWKGHYRNFSARLQSEAHVRHRAYVPAARRRSTHAGDPTEGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR44</accession>
    <name>A0ABQ5RR44_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001971</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="138" mass="15827" checksum="BB16EDD5D9C4F77B" modified="2026-01-28" version="1">MSVYSLYVINKSGGLIYNRDFCEAARVETNDSLRLASIWHSLHAIASQLSPVPGCTGIELLEADTFNLHCFQTLTGTKFLLVVDPHASFIPALLQRIYELYSDYVMKNPFYETEQVIKCELFDENVELLIRRYLQQVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRN6</accession>
    <name>A0ABQ5RRN6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="100" mass="10144" checksum="E2F7FF50CFA608C3" modified="2025-10-08" version="1" fragment="single">MDTSKGMGKHHIGGTSRLGASWSIEPQLPPSPSLAPPPPPRSTPIAVQPHPRATDATSRKLHEGRGDPIRGGNVGTGWCGTRVAAAAAAAPSASGVRAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRT4</accession>
    <name>A0ABQ5RRT4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="227" mass="25324" checksum="0641675127845F75" modified="2025-10-08" version="1">MQIQALNQSLCKPAGSVQDSRRAVFPIRCSATSSNVNNAVTSISIRPLLLTQPARPVMGQPRALRGHTTLCQSVANGKLISSTEVPAFIPRDDMMDQLFRWSMMEAGESGQRNFGMPMKIEAVYYEERLWGFNVAIFKEGVKLTDLGVMFDKNAITKHEWVGRGEDGFPVMEGKTDEVKGKNFEIWKLDSEPVSEDLRSTIRAYCTALVAALNRYYAFGSVFVDDAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRZ2</accession>
    <name>A0ABQ5RRZ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="2">
        <location>
            <begin position="1025"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="863"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1139"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1481"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1649"/>
            <end position="1681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1798"/>
            <end position="1859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2074"/>
            <end position="2104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2281"/>
            <end position="2306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="863"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1166"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1517"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1649"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2078"/>
            <end position="2099"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3142" mass="319964" checksum="29F86D2D8367D6FB" modified="2025-10-08" version="1">MLLLLHGCQWRVLQALHHAVVLLCIYNINSASGHFCWYPTIRSYATCELPSGRSNVSQVLEPILPLAAGLRPCLPAYLALARDHSRRALLPGFDERLSVVLRFDPWAAAVVPPNSSLATTATVDHISVPSAVTNSNTVLTSDSRSSVPATTAAAAEPIAPGSARASEGAAAKTVSAAAAATVAAAAAAGIISVGPVEAAAVAAAEAAPEAAHAVAAIQDAPQPVLSVLGRMETAALPLLRSRNDTWLQQLNTAAAAGDPLPMQLLPAPTGRPGGPPAAPLLLFYRRDWWAALTAPPDVTALSTSDIRMANTSTAAAAPAAANAANSNATRVAGPPPIPLPPTWNLFSLLVADLVNRDLDGDGLPDHVLCIDMMPGCKGGALLAAIYAALAQANGTSQGLWFNPLDLSPLLGGPALVAAMQLYGALGAANAGPFTPGRRIPAGGGSLSFNGSSSNNNCSIGNGSLESAGGEFVSGSISSSSTVPVSVEELLAGGGEVDPASGAPVCGAINPLFAAGRCLFTVDWASAATWLEKGSSGIPPGALGVALLPGSERVQPIKDYNAAPTVSSSSDGGIVGSDSSAVLASNTTSTNASASSNGLLLCTATSCPYADAVNRTFLETVAIALNRNVLRPTNSTKNGYGYGPDSAASRQQSETGPYDSSALSLKYLREVRQMSDRLLVNRAPFLGENEDVWLLLPPAPLPISSQPLPPASSAPPLLLPSPLLQVAQQTRAAEFARLVSYQLYLKNGLITQLSVRQGIEGDPRAMADAAAPPPTNNDWNAAAYGNITVLGGVSQAVPPTGDVTAAAVASLLKMDPDDVMRVAKVVSSAVQHRNAAIDIQLPYSSEYRTVLDHLAEAAVAVRSNSNSGAGNGNGSSSSTRGAEAGSTGASGKTDAPPSQAPRREAVAEVAVTAPTSGDALMALQSWAVQEFANITQHFPYPAILIRIYAHTIGAVRPPRSSPGAPAQPPPVPPRRPFSHGIIVAIVAAVLAAVCLGVGLIWRFLRIRNEGLLQGSGRMPGAHPNTTLVVTDVQGSTTLWEMLSGDLMDEVLHIHHRVVRQAISQWKGYEVFTEGDAFAVVFHLPEDALDFAVGLQAAFLGADWPPELLEQSDCCEVWARRHSAQPPSSNCKRSLQADLMFPHGPPGGGSGVGMGPAPPSGPLAVSTPGGTRASTATGDRDDSTGGRPGRRILNVLSPFWRKSLESSSGLDLGLRPGWGVMGRFPKLPTAPGGGPTIPTIPSTSGALSFLNRGTTSASVPVLSPGAISGCNGAQYGTNVAGAISTGMNRWISTPLNNVSPVPPALLTRMQTAGTPMVSGANSRLYMSQSKALTATRAAMAPGEVTGTGATTRLPPPPYTQWSATDGTNSPVGAVGDVGRMISSAATAAAVAAAADAVMGSGGDGGGGGGGARAPDRTTRNRASRVVPYFSDTVWGGTGGPCYRSLGECGAVSDKTTTPNVAAIASPGAIATAATTVAIAASQQRVNSPKEDGEAVAGPLPRLPVGHLPPTQLQARRGSAASTDPPSQQLSSSYGAASQTEVVAEAGAGAGPLDVMGCAEDPGGKPREKTLVSEQPGPCVSSELRSLSGLVCDGGAGEAECRISAPASAAGPHKTPTQAFPTSEVPWIQKQFPLGLLPKGLPPWQLLTQRLQETQKQQPQLPSTEGSRECVRSQEPGSMSVPIDSSDFAYRKQNKLSKGDRSSSVFPCVCNGLISYPLLNKHHYHHYYYELSALAGSPRGDPSGALYRKSCGTAALMSRGNDGTDLLSQSYGNSCAAAGGGEDAEIRTGEETCTHSLTVSLDSDDGRSLTEPSGGGGGAAAIMNSLDVDTCMDPVGAASHSANPPKPRKSPPAPTGTQAAAATATAAVAVPFLVAGRIDGPPSGQHSVPKPGVAFGVRSVGAHGSDVRRLLHDFRDLGLGPPSTAAVVMEGGPAAALDDEGVGDAGDWRSCVAWDTSTSYNTFEGPDRETHCSGSTLSVARSTPPLPSALAPLSSPSAARAVATASTATATFRRPPPLKLLSSNRYSNYQVIGNNSQQQQEQHLLPAGSCPPVGRLCSPDQTGPRSYLLGFDRSYGATKARSPTTNRQSLPSSPSGVLSQAPVQDRRHVSVGRQGLPLVVTKRAGSAIAVDGWFSRRRRLLLQPAESPLRRTISAHRSLTAGGRLDADVSAFRRAAGGEEDGNFLAGVAFASGGTTSTQTAGGTIFGSGSPRPAPQAVVAMKQQQQDAGGRGLLQRLNASLHRMSTLSRISAGSAGLWASVSGIGASPTAAATSTAVATTDMDLPLSPSQPTDCGDTATAAGQDDHPAEEPPGGLLALFNGLELGIGQGAAAASMVTAIRSAAAAAAAAAPAADDENDDVPPATDAATKPDHVTVNMLTTCTGADSSAPCAAYNAADTCDIPHIDTVANGTEDATEATVSIATHGGPMVTPTTVSATCFRFMDPAAAALGPSTSRAAVNANSNTAAAANGMSTSLALMREHMLYHMGRSSRLLMLGQAAAAASSAPPVATSLAAGASRGSLFMDLLWRDINTAASGALGSGNTTANGGVIIGHASAAATPAADGTDISTNISGVASAPLQRRRPEGWGLRNAVTSVSTGAVGAAAGAVYTLASALKSIYEQVNTEGFLAHLGIVGTLGGVVGCGGSGIGSVAGPTGSLSTCIAEPQLVLRGLRLRVGLHSGPSQAEVVFLAHGGVAGVRYLGRFLSTSKDVCDAATGGMVLASGATFRAYQQRLQETREARGQRGGGGSKFDLLLIHLGEYLVGPAPAGGSAEDAEHCAVKVGRSCRPVTAVNISAGPNTDSTAAEDASMELYGVVCPWLVPRLAVLPSPVRGLRQVVPGCLSAPAGVVTPVFCNLLGVEALLAWEHVVQQRHLSAAMAAAAAAATVTATSAHRTSPEASSQIALVRPPGTYVGDGGCGAVVQEALELLRRAAMAAAACHGGYVVAVSADGGHWVLVFGSASSAVSWGLYMLDAMLEMPWPDGLLEHELTEEVYEGGALVKRGLRLRIGADRGCAMLRPVPRTGRLDYVGRPMNRAARIAAKAKAATMFVSGAVWDAARGYLERPVSASCLGLSQLKGVREQVELWALRPVSVAARSTPAAATTTTTATATIGGGCVDPAAGDSDETVATVVVGADAANFGRAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS12</accession>
    <name>A0ABQ5RS12_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alkaline phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008557">
        <property type="entry name" value="PhoX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35399:SF2">
        <property type="entry name" value="DUF839 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35399">
        <property type="entry name" value="SLR8030 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05787">
        <property type="entry name" value="PhoX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045945491" description="Alkaline phosphatase" evidence="1">
        <location>
            <begin position="25"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1314" mass="142046" checksum="EDDD546F9F73E851" modified="2025-10-08" version="1" fragment="single">MRMQNTAVALTLALAVAIAARVKADLYFPNIPPAISEPEKHSVMSVHHAIVGRGVALKPNAAARANKMAGGFQLLTRTGTPDGPGQVVDIGGAPVMAYGTGYAKGATPQDGSVNPDSTTLLPRVGDPTKADLFVHFESPLPASVYHFELDVGVDGQLAVRSYKPVDFSAWGGVWTPCAGSTSPWGTHLGSEEYEPDARAFYSSNSPQSRVRDHARYFGLYQGDFNNSTAFMEALRQVMSPYRYGFVTEVSYDAATGTPKARKWYSLGRVAVEVGLVMPDKKTVYITDDGRNVGFFKFIADKPGDLSRGRLFAAKFTQLSAANGGNFTVTWVALGRTDQDVIAAAIPGLRFADIFDYATPVGGSCAAAGADFRAVKHSYGEECLRLRPGADMLAAALETRRFAAYLGATTEWSKWEGITLDTRRRKLYTSITDINEGCLAEPTRFGGQDDIRLPPNRCGCVYTIDLDPSYNAIQMYSLTCGIPDVGITPGLFVNYTCHVDRISSPDNVAYHRGLDLLLIAEDTDNHENNFLWAYDVTSGDMTRVMSAPVNAEVTATAFLELPNGFTYIWNNIQHPYEGLPDSVANLDDAAGKGGYIGYFGPYRLGQGEALGLQAIPAPVSTASKLTLTSSSKAVFGTYVPASYKILARSGQRFGDVVWGQNRDANLQPVAEITSSWQLLRNKSEVSNSPDFTSLTTVCDKTFYTTQFEYANPASMYIQVLSQDPRSGQLSATSKAAFVDWSAWGGLLTPCAGSITPWGTRLLGEEYEPDARAFAYAQRLEDIGGGPNATQFTYDGGNAIKLARLYDMYYGEMNLSEFKAAVKPYLYGYISETKIQYDKSAIVQKHYALGRLSAELGLTMPDKRTVYITDDGTNVGFFKFVADRAGDLSAGNLYAAKATQVTGSGGGTFRITWIWLAHGNQDLLMVAASAVQFTDIFDSELPAANGSCPTPGFRAINAAGRGCECLRLRSGSEMFAAFFETRRFAAYLGATTEWSKWEGITLDTRRRKLYTSLSDVRQGMEDYKDRGATSGKYDMCGNNDIRLEYNRCGCVYALSLDSSYSAYWMEEFVCGVPAINPAAPGFVAGNRCLLDRIASPDNIAYSPEHDKLFIGEDTSEHVNDVMWAYDLETRSLTRIFSTPYGSETTSTYWYSNVNGFSYIQATVQHPYGESDTEKWTDPLSFGLAGAAGVIGPLPAVGGASGSAGSGAKCASQDTFMVEVQVVYERTASSGLSDKDSARLDAYICARVDNTLVLLSGGDNDASRVAVRCVASEAVNSSSSGNGLLRGAVLARVQLGSSAAVAKKFRNAASDPVGSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSC0</accession>
    <name>A0ABQ5RSC0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002641</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Protein transport. Probably involved in vesicular traffic.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01861">
        <property type="entry name" value="Rab6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="211" mass="23718" checksum="66183465DEA546F1" modified="2025-10-08" version="1">MSAPTTSPLAKYKLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSSVAVVVYDITNRQSFLNTARWIQEVRTERGNDVIIFLVGNKTDLVDKRQVSIEEGDAKARELNVNFIETSAKAGLNIKALFRKIAAALPGMDSVTQNKQEQMEEVKLTTSTVTLDAKPPPPASTCFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSF5</accession>
    <name>A0ABQ5RSF5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002241</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045551308" evidence="3">
        <location>
            <begin position="17"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="721"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="776"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="868"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="979"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1094"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1143"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="654"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="721"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="776"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="795"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="825"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="880"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="982"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1094"/>
            <end position="1105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1216" mass="123182" checksum="1B50870CFBBB9309" modified="2025-10-08" version="1">MMKRFALALFVCTVLAFPRPEARDVPRINTTTAGRKGKDRRGDRGNDGTTALRSCADSSSDCIRNHSGHATNRLRQTQVHHNTSSHNSSNSGSDNSSTSSNSCQRQAPYPRGGSLRGSATRGAAKTLRGNGRGGGGDQGQLPPPFDRGGNGGATTATIATGEDLRLRRSVVMAIYRQHGDHHRRQVLHDFAYPQYPPHAPELPGAPPQLEPPPSAPPPLEPPLPAPLPAPRPPAASQSAPILPPVGASSSPPLLPPPAPPPPAAPAPAPPYPGNASPRPPGALVPPALAGTFLEQVPPPLLTPAPPAYASQWATASVAPAAARPSSGSAEPNGPVGLLSALLPPPPLQKLSTVASRNEPPLLDSPILTSPLQPPPYYLTVHRRGMAKFDAILAATAGAVGLILLLMFVFMMLRIRKALRSRGHNNSACCVMEAALVRAGGGGTGAIAVVRPFKGVAFDLEGVTDAEMSPMRFIGSGPAAAVPVPTGKKEMGSVIKPRSIVRGGTVTGVAGMLSSKKLGGSAGGAAATGGGVAAGVNLDMCHNLLYDMYDTDNSPDIGRRGVPPALQRHMAAQSSGGVGRIAHGGGGSGGGAVCVPVRYDTEHVVRRLTADMSLPSPHGYSAAARTVASRTHESGRDIGPMGQCSGDTTARRGSLDQNSASPDDQNTKGRGTTEPTAAKSRTTAAMAAAAANTRRRTTNDEDAYAVLVVGRYHCDSDLAKGAGGRELHSSDSDGEHRPSLGEGRGRGLTPRSSIGAIGDVEAAVRFTTTRCDVIRHTGNTHGSGVSGRDNQLDSCNGGGGGGGGGSAAARARVRHGGLSEGGAVDGGRTAAAPRGPARGASSYAGVPPVGGASGAAAAAIAPAILRLKRPPRTSSGGGGSYISNGGMATARSVSQPASSSASHQDPPQTASSAVRGGVPVPWHVIGSAAGSAAASAPTPAASPSPVASAVVPTARRRYHTDGNGDVSGSDVDDIAATAADTGANEDENEGGGDGDGDGDSDGTATPASEVVSASLLRRLLVQSGQSGGLFLRRILASETAAAAVVGASKPSPRATLNHRPMGRSISLLAAAAASNDGGCGRKPGGAELNAMTLGSVSHNESSPRTSSAREKPVPQRHRLSQSSGGHGSKEYAVRVVDVKKANVPMVHTGVPSGKVTASGGKDREGEGDGISSNDSNEFAVDVEAVQQPLASASPRDQQPLPPPPPPQQQRFRRPPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSS1</accession>
    <name>A0ABQ5RSS1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="399"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="585" mass="62059" checksum="11B97444EC2167D5" modified="2025-10-08" version="1" fragment="single">MPCCGGHSSGHKVSTPLQISWTHGVGDVAGEALLNLSVLKTLSEHVDAATAAACHSVCRAWREAFGLSCLNSTLCTSEPVDGTRARFVANLHGAVTLEVTQPAEKAQRGFERHIAALSRNRHFLQLKVHLPVREATEQQLALAALCALKPLTQLTSLVISRNPAFTSATHNLTEEDIDALSSLRNLNFLELCTAVPPPLGLSQLAGLAARDLTQLRTLAFGHTSALWRVPDVARLCQLLDVCPVLTDLRLRCCAPPRDAKQLPDLMALPLPQRLTNFALTFARRLGRGEGGGHGGGAAAAAQLLGLGGAAADDDDEVQYGEYEDMDEPWDSVDHLAPKPGLHVLIVEKGTRITRMDINVTISVSASDLPTISLPGLRPRQQQQQPRQQRRMLAIFGEKGQRRHKEALKMNDDDGDDGDDGDGDHAECRASDNGGADEEEDVPPYDVTPLVLSEYNRLLGQELGLTGALGEPEDLHLGVRLDILEDEDDAFAAAAAAAVNHIQHMPGGAAPPLHVLAHLHAALQAVDQLHNPQQQHNQGAPGQHGGGNNAMLLAAAAAAAAAGDAGPNGIPGQVGNLPPAIAMLMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTC7</accession>
    <name>A0ABQ5RTC7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_003071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="41"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1081" mass="123158" checksum="DC1BA5C44A5E8C97" modified="2025-10-08" version="1">MGDLAATLLDFNKPIDVGLLDATVNLSMQSVVGGEQQRAAAEKLLLEFQEHPQAWTRVDTILEVSQNQPTKYFALQILENVIRFKWGALPLEQREGIKNYLSNLIIRFSMSEELFRKEATFVNKLNILLVQILKHDWPARWKTFIPDLVAASRTSETLCENSMKILKLLSEEVFDFARLDLTQAKTKELKQTLTVEFKAIHELCVFVLNNTRKTELIRTTLETLSVYLTWVPLGYIFEGNLLELLLQLFPQAPFRNVALQCLTEVGSLQMSTEFNPQFAKFYDFFMQQLEVVVPPTVNIPDAYEKGTDDQQKFVQNLALFFTSFFKAHIGILETLETQTQLLKGLHYLVDISYVDNTEVFKTCLDYWNFFVPDIYTSACTGMDPTGVAFMFAPPATPGVEPPLGGGRKLLYRPILSRLRQLMICRMAKPEEVIVVEDENGNIVRETMKDNDVLAQYKSMRETLVYLCHLDYDDTEQQMLEKLRMQQLAGANWTWAALNTLCWAIGSIAGSMADEQENRFLVTVIRDLLNLCEVTRGKDNKAVIASNIMYVVGQYPKFLRAHWKFLKTVVNKLFEFMHETHPGVQDMACDTFLKICNKCRRKFVVLQVQEREPFICELLNGLTETIQDLQPHQIHTFYEAVGLMIGAESDSVKRDDYLHRLMGPPNTTWNQILSQAHANPEMLRQQEVIKSIQNVLQTNVSVCSSLGQPFLVQFNIIFGDMLQVYKLYSEMISQTIATGGPNAARSIFVKYMRSVKKVALKLIETFVDKCDDPQLLASAYIPAMMDPLLGDYARSVPDARDAEVLSLMAAIINKLRQLMEPEVPRVFEALFECTLTMITRNFEDYPEHRLQFFALLHAIVNSCFNTLFMMSAPQLKLVIDSIVWAFRHTERNVAETGLALLHDLLVQFAGSDYATQFHQTYYLHLLNEILAVMTDSFHKPGFKSQARILHHLFSILDANVIKAPLWDVAAKGAGAFPTNSAFVRVHVIGLLSTSFPNLTQAQIAACVSGMFEYKDFSAFKHHLRDFLVQTKQFASQDNAELFAEDVEKQVVEQREARQRVLSAIPGMIPQADTMAPEDMNDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTX8</accession>
    <name>A0ABQ5RTX8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003328</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="96"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="448"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="517" mass="57051" checksum="AE10CF16D73A4D4F" modified="2025-10-08" version="1">MPTESTLPQGNEAADRLCHATCQPSTSQGRSLEEQNGLLKPSRGRDGVRNPKAGSTPVAAASPSMRTPGLNAYPIQDLRPENVAFCDAHKIMDGLCLQQRELGRGSYGIVVPGIYQGAKCAVKIMLSTGLDGAAVRELLLSPRLVHPNTVSTYASRCAQLTHEFFDLLEGERSVGYDPNFPRVLEPVPLKSEDGFGEPRGINDGSDPLLVLHQVLYSLGADTGKMVVVIIQEYCDRGTLESAIRKKVFRATPQWGVRLARRALLRSAVEIARGLLHLHDWGVVHGDVKPANVLLGSSKEDRRGFVAKVGDFGLAHVLPSDASSLYTDTSGSLAYMAPETFRGKVSRATDVWSFGVCLWQMLTGERPYDGYHYSELMFGVQEGILELQWPVDDPMTAPLIELGRQCLSHRPENRPPFDVIIQRLVNIEREIRAELLSSQQQQQQHGGQQEQQEQQQESYQQQQQQQLQQQQLQQQLQQQEQQQPTNSRASRAAGGLSAPVVDLPTAAEGHFITDLVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUA8</accession>
    <name>A0ABQ5RUA8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_003454</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF702">
        <property type="entry name" value="AMINO ACID TRANSPORTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="532"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="69"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="581" mass="60386" checksum="F1B70D07EF048BEF" modified="2025-10-08" version="1">MSAQDGAAVEHKGVIVGASAQTVATGTRVQPVVMASSEPHCSHAAVLIEDDPNYISPFGKDSVAHALYSALVTLTLGVLGSTVLPVPYAFSKTGIAAGVLTMALVAAANDATCCMMIRAAAHTGLTTFEQLSEWAGGHKARVFTQVSLILLLYGTLCGGLAFLSDVARVMVQKGLRDGEAPALLAADGRPIMILTVLTVLLPLCLQRHIRQFEKAATVGVVVVVVLCVIIVARAVADDFPAVRSGELNVLDVKVDAHLPEAFAVLGFAFYMQPMLMPLLKEMPSGPTGTRLTERAVHITLFAVACGVYGTVGVFGASIFGMETESNIMVNDLLPGFRTVTLVLYASLLVYLCCGMVTTHYALRASLDLLLVGPEAPFTWTRQVAETLGILACATTVALLFPTQAEKIFALTGCTAVCLVCYVIPVFIHLAVQRDLHRMMDERGGAVGTPAAAVATAQAADSLREPLLEVAGPELGAVTGAMASCFCCSKDAGPKHSKSSQAIAYRGGNSRDGGSCSSMSGVLQCVWQSLCRYALPAAIVAIGVGFSLAGLYVGFVDLMSYLGGGGTEAAGSPPALAASVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUZ1</accession>
    <name>A0ABQ5RUZ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="222" mass="25174" checksum="97EA7C65BB57338A" modified="2025-10-08" version="1" fragment="single">RVLHETQIIMEFCDRGSLQDAIEQGVFVVDGPRGARGGTNDPEVDMELIIMTLAEVSAAMEYLHKHRITHRDLKPKNILLKTSNKDRRGYVSKVSDFGLSQVLPDTNKTQVSTKYSGTVTHMAPELIEDGKVYQQGDVYAFGIIMWEIYTSSTPYQSMAHPQIMVGVVTHNLRPKFPTNCPEWYKSLANRCWRKDPKTRPPFTEVTKSLLAMLPDKSWSATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVR5</accession>
    <name>A0ABQ5RVR5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004140</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="20361" checksum="4B0B340E548F0103" modified="2025-10-08" version="1">MSNVDEDRLASSASASTSESGVQPSSAGAAPPARKKKEWQPISMPTQQELAAQEFMNNCFVKSAMSGAMGGLAGFAFGLFSASMENAHGGLDTVPEIGQEKTTRVILREMFQNMKSKSLSYAKGFALMGALYSFNECVIEKWRAKHDKINPALAGCATGAMMAHNAGPTAMCWGCASFAAFSTAIEYWMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWD6</accession>
    <name>A0ABQ5RWD6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004465</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="17"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="94"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="211" mass="25396" checksum="B7D321BF8A29C03A" modified="2025-10-08" version="1">MSYRERGGGGGSRPPRVSLVVRNLPLDIRAEDLRAKFEKYGELKDVYIPRDYYTQRPRGFGFVEFKDTRDAEDAMYSLDRSTINGREISVTFSREGRKTPRDMMKIEARQKGYDDRYDDRRYDDRRHDDRRRSRSRSPRRHYSRSPRRSHSRSPRRSRSRERERDRSPRERERDRIPADEDMGRGRDDERSPSRGRSPSPRRESPRPEIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXV9</accession>
    <name>A0ABQ5RXV9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_005030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF31">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN CEP250 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="3">
        <location>
            <begin position="7"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="149"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="419"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1291"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1576"/>
            <end position="1608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1639"/>
            <end position="1661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1848"/>
            <end position="1868"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="672"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="725"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="802"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="863"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1359"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1661"/>
            <end position="1744"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1788"/>
            <end position="1836"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1871"/>
            <end position="1970"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1994"/>
            <end position="2120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1305"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1339"/>
            <end position="1354"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
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    </evidence>
    <sequence length="2134" mass="230829" checksum="1B41A4D36E890A3A" modified="2025-10-08" version="1">MFGKILRAGKGGYGVKYKIDLQVVQLENLPTSIRKCRVVWARSAKLQMTDIKDARGSVASFKQTLTQVTTIYRDKAGKYEPKEYELKVQVPGKGSTESPVTVGKAVLDMAKYCKDETTTQSAMVPIAFKIGASITGYLKLSITTIFIGDSNEDGGTDVSGLTGLTSDHGSMREQDLEGFGDEEHVKFSKKARGRKREEASTSATGSSGRSSGNNKAEGPWRHKPLPPTAEEDDSDLEDKQEAEPALTTVKSSRRNPSAPRAPEIEEAEEPNDNPFAKKVSKPTVRKVPAKVWEDDEDVSPLNSDVSDSEPPKPTARPKKVEEPAQSLDELKASLFSTKKTAGSAVTGVTGASPAAAVSSSKGGSSKASKIPPPPLPIEDEDDESQRDDLFSAKPKGGLVAASNKMKAAAATAIPANAATTNAAAATAAASKQPKTRVATPPPPEDDDDEDEDLPPPSTAAQYIGPTQKPRSKAVESSEAAVAKGSSSLTRAGSKQDPSKGGSGSELEQLRKQVAQLEKQLLDEQVAKDDLNKKMQKYADKIADLEDQLAEGALMELYAQMKEMEEKHQAQIKDMEAKYQTEIKELMERHLTEVAELDEQHQAEVTQLQLAASESGRRRGGPSVEEIERLKAELAEQDALFETRAKKLAAEQAAANAAREVAETKLAAANKMIDDSLLAATKHQQENKALREEVARLKRQVEDISMWDTGVAAAAATAAVNNGRRDSAAEVELEELRERVEELEEENTRLQKLNEVAQGTAASMLSDIRAVHPDAQGGRDFWSRTDSAFGKLVIEVRCLCDAYTHQSSELRSVQERLTELSQQAAAASTRTSASGELGAFDGGFAVSGLNTSGKVKPHELLSRVQQLTAERDSLQDKLANQRQAQSDLVTAQARLTAIQADFAATQNELATAQANLAATQADLEAAESDLVAARKLVEELRQQVDTLTFEKEAVERFKVATIADLRRQNDELRSSLRSLSSLGPSPRIEGEEDLLPAATPHTAPELRAAFATLATSRAASAAGDSVIEGNWHEFEYLVAQLKADRTDLRRQVEQLKADKAVLGKQVEDLRSAGGALQVPAATASVVDMPGLPRQPSRRHAEDLELECSNLRSEITRLQAQLAELSEADAIIKEWSDYADGLVADKTRLEQQVQELTQRQDSGSLSHDLRTADTERRIADWQRRAGEAERRATELERQVTALQQRAADAASRAEGLELQLREAERRVEDAESRASAPDSASIMATAAAASVAASPLGTPRQLVDTTEVLELRRQIARLEASLSALRMQLIETRSQPVSAREDAPLTARSQGDPSDRSTSQGVLKIAAGAHSVEDDAGNDATSRDGRSTGSGHDDRGWHEELEQLRARNASLEATVAGLQASLEVATLQAAQGSNSEISVREAKDQIQELQNRLSLVLQEKAELDSTVVLLWAELERAPLVSHESGVLVPADSAAEGASVSYQKAAAAVRTAAEDVVAAAASPDIVEDVAAAGLQQELEALEEEVEKLRRERSELRAQVKELLLELRRLEDAKEEAEHVASRLSSEMEELAEALDASNSENERLSLEVLTLRDQLEQLEGGQAAGSASGSRSQRSSEKSCGSGSDSDDSALLQRASLRQVDAERRELTAVRAAARDLIRQVVAGGQNQGGTEEDHDPSSGLSDGEDLAHALQSLRAQIAELQRQNRTLQQATPRRSSLNAASAAATVAAEAQLAEATARIDELQALLEDAEIENQHLTEQLEECLTGGRKGPDSVGSAAEECQSSYGGSAAAGATGASNPYSYGHANTLTLSAFEQRLAMVESQRDELSANLAESSLTNRRLEAEVAALQHELTAVRQQLDAGTSGALERTSLQVSTRGRASRAASDAEDKKRFDELQAANRKLEAVCHDLEEKAHVAQEQVQTLETELEAAKEKIRSLEAAAATLQKRMEDEAAGAGGRQSKEKVADLEARVSRLEREKRTLEEQLEEEWATRKQLEKSLKNMLDRAGDDEAAETVAAFEVKYNRLKERYKMLEESSQEELDELQDQLTEALSQVGDLQRQVKQLQSQAAQTRSAGAAGGAANVEELRNLRSENERLVQQLVSKTMELAELSESEITIKRELMRLQEVNRKLAQKATTLEAQAAVAQAGKPVKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYH2</accession>
    <name>A0ABQ5RYH2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
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            <begin position="944"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="EMBL" id="GLI62454.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
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            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2984" mass="302339" checksum="A77AE93F41034186" modified="2025-10-08" version="1" fragment="single">MAAGTPDEKFQKLREENERRRKKKEEEDKRRKQEDELKAKEALERRRQERLEQDERMRKPWQQPPAPKKPILRKGAKQKPIQPTSNPIDPLRQSQAEEKRREWRQGLQDFIKQQRQQAVSAAKAPAAAAPARPAGTDGAPVVPLTWEELRMAAEAGFDELLRQQELQAEQCSVEEPTARAARHRSPFKSPFRGAPAPPQETVQVALVPTQQYTPHGMAASASTTTSGPTHGSPQPSTRHSPEHDITTAAATAAAGSTPHGYKYSDAPASAGPFAPGPAPPTAPSRPLPASPHPPPPPQQQQQQQQRQVQANDAPDWRPDQHSVDTATGDSVIESPPPEAVLHPHSVSNAQARGSWMDGFAGRTGDANSGDGGGSGSGGAGSSAAGAMGSVASSGGLRRGVTSTARYRASPLDPSLLGLAAQPSAVDTTTDPRASGANFDTDRFTDTGSPQSPSGSLPPLPGPYDVPSQQQGPPPGTTPPHSLAAPQRGPMQQAQPAAGPTPRLGISDDPLEASLQAAGTLAALQQADGFRLSSPRPAGQRPPPFGAAPGAPPPLPQQSQQFPAAPAGWPQNEYPDGPHLHGTPPPPQAQPAKQDQRQARRGWGMPAPPPHGAAALPQPPLPPADLQPPPWARDTSYSPEAAPPSGSDSRSDPDVGSRVESSALPSELPLPSAAPGQVRSPVELRQSVAVGDSGGPEMWQRDSITVHPVSGAPAAATARHPNDGDGATVGLSPPPSALRHGDVQLPRPMMTPSPRSESGGGGGGGEPGDGQQPGQPHSGVGLANWPQTQGQGQSSLMASGAAIRVDMSADLVRFSSPPGPSRTPSANSGGALASGSSQVADCDDNLEVLHQQAETEQQQQRHRLSCVSYEGAALADSAVVKPTKEEVPLRPALNAGKDGGTQHTAASQPGPQTEQAADAVQAAANARLTAPEPLAPLGLRLTASQGVAMPHGSPQAVSSTPSQLSSQGLLPTLPQAPLLPPIAPGRPLSGSAARLSSSGYSAVPPVSLPPLSPQSTAGEGEAASALAQLHALQHHQQQQAPFRAPSPPASPPRNGGTSQRFQRQHSGTSSLAISGSMSGGGGASASAAGVPPARSHSYDGQGAPSTAATATVGYVAVTAAPTSPSTSHFQPEHEQLHARQQQQSPINSSVSSSCQGVHSGPMVNNKTPDPELEGAHGHLVADVRPLTRSSGEAARPETVVASQPGASLAPRAAIPRSSIVQEPPVVVAEGPSEEAATGLTPLNRRPMDPVPISLNGPAGAPRDEVVFQGARRTADTEVEVEDGELEGELEVGTEVEEGQAEEDDADVIIDDDEGGLGRRMAILEGSSSLDRPPGTAEGSVLTGSHISARPWSGPSSTGPSGGSRLAGSGYQNSLLDDSVSSFDFHMRSGAACRPTSASPSSSSLPPPSATAASLLYPAAAAAAPTGSASTATTSGPSVAAVGVPVKSSGSQGSRVAEGPQDATGRQASAIGDAGWSKGRGSAVTHGSAVASNKALRRLPAVFGGGGNGVAAAAAASNTLQVLQQPAVGRTSSSSEMNMGGVDGEGQAFVEEPVEDGYGDAVAAAGSASTAGPATITTPLMGAAAPSDKPDPDLDALMREIQELSALRKALKAEFEIMGRGDAVAGLGTLSVNFVAPPAVTASGAVDAPAPDAEDVYIDTDIKLQSMPLPLPPVPPQQQQQQQQQQQQTQLPPPPLPPPAATRAPLLQWNIPGQSVVQAKAASATAAPTVHTPAVVAVTSPEPASKAKPYTQPHMVKQPLLSPNLPPSSTQPLSAAAQWDIQNQQQVVQPQLQQQHTLSPQPYAGQPDLLSPPLQPQPLPRQLVGKEAQDQLGVPGGQSTTPQLPVPVLAAPPHPPSYIMPPVVAAASPPPPPITHGLPGLAAAVPPQLPVPSMAQSPSQGHMPTGSKVATEANAPRVTRPVATPGSGTASSGEVDAHSGSFAFSPPPGATLGSVGSIAGGVVAGGAGPEQAAPVVIPAPPSVPVAPVLQWTIPVNGFVDGATTAARSIGGATDATSTPAAGGPGPAATASPSGRAGVSTRKNAAPQPKRSPSGGGYTAPGSAGTDSPGPGSGSGVRRGGGTAATHMAGDATSMGRQAVPIAMTTAVAPAERPLINEGSYAQAAVQARERADRERAAREALQAESAAAKAAIVQRSKSDRAAAGAASPSLAGCSKEASPLGPKAPSTIGSYVRHPSPARAGPVAAAPQRSGAPGDASGGGSIGTPASEAVGTTPAADGVKQAWGTPSSTGRPSRIPTPPRPLAAAAATAAAPPPPASTPPRPAREDSLGSDFDFLERASDTSDSETESPEAYGVSSKVSSPPRPPRREAFASPAAAAAASTAYRERLAEARPQPLRIPSSAAATPMGGASTASTVASRAVPTGGRGHGAARPGGKGPSTPFTAAIRELMGLPGSAPLEQSRAAAGKQPQQQVGRAQVLPSRGPAGGQMQGAAAAGCVEPIRLLSEEEDKLLASLKRLDADILRRGLMAAGIINGPLPSQPPAMAAPGPAAGKSVKVAAPHQGGGKGSSPALLTNSFQQDQLRESLERLDVQLGALRRKMEDRACNLGSPSPLGVASSNGTPRQDGPPPPPREERRGPPATLAKSRTPTPNKAKAIRDQRQQQLSAVQGPTPPPARAAAAASAAATPQPPKPASAPTMGTMGSTTGPEASPMAHLGAGGSGSATTATLAAAGGQPLGMPPRAPAAKAPSSAPSGPAASNAAAQQQQQQQQQQQQQHQQHQHQHQLLLQNYQQPALPQGSTPFGVMPSQVAFGGPAGGGSAGGGIMPPMFAPSQPQSQPHQIPPGSASALSGQHQQQQQVAYVPAMLPNGQMAFVPQYVQPSGAIGAFPGVFPGAPVPGFVHAGAMHAPQPQQQQQQQQQTMMPPMMMMMPPAQVPSGGSSMLQAMPIQQCQAYPQQQPQLAGSGPMLAWGAGPVPGAAVPPPPPSPQQQQLLQQNAAYNSSGNNNTAGPSLYSFDRTASAGHGMAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYM5</accession>
    <name>A0ABQ5RYM5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="127"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="415"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="477"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="538"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="662"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="735"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="796"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="839"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="944"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="494"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="575"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="611"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="679"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="752"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="849"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="859"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="898"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="946"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="978"/>
            <end position="991"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="991" mass="101564" checksum="A9D9C5D611632E6C" modified="2025-10-08" version="1">MQQPHSSTLRIVRLPAADSGYNLCASAREVVLARAHPLLSGHVGLVDCQGVYCVAKLGEGDSRASVTAVYELQLCTGAGSAQPLHKEWCDFQLLPDGGLLLSQKSSARILFAQLPLCEGESGPVFLFGCHEGAVLSLATNRELLLSGGADGCVRLWGLDSGSLLDCCRRQGAVGGHVTAVAFAGPLLVAAGTDQGIVCMYDISTSRLVVVQRFTAGEGPITALSTRLTPRQVYDVATAAAPRGPDGGGVLVAPVGLPWRSSPGFGAGFDDGSGTDIHTRSDTLLAVASPAGWMKVFRAARSGEDAWQVVHHCTLDGPPYLSYSPDGNYLAVGAGERLEVLNATTFELVHRHLFQRGPLAGAGFLPASAGGSSLLAMQLGGSLAGGTRLRLFAAPAGMDPELVQLTLPLLDLGIEGHERRPETNKDERFPDSLPRAAINNPATSSRRATAASALATTSELLGGLSLGSTCPTAAVTNEPVDVRRSGATDGSGQQPAALSSPYPSSPSARRSLPWGLGGVDTGIGTELSGVNPAWGWQAFAPSPSASSESLGTGGQGGRAAAATSPGGGGAKGAERSPSPAEPWASPSWGGDGAAAAVAVATASSPRHPLGSWSRSPPKSPAVTTANTRTGTHGSVDMPEGTEANGAASAAAAHAWQAYRPTTQPAPRSWITGLPPRADFLPQPVSSPLSSSSPPRRQPAAPSRALQGSPVKAAAPGAGAGVMEVASPTTDCFGAAGAVPHPVSSGRPNWEGFGADRTRGREEGPDVEVEAGGLSGVLWHEPECELPCSATLLPATSGGAAVTAPPSSGPSHRGEGDSGAGPQGFSTGAAEVLEATFRFQQLQHQHDHHQQQQISTYQHQQQREWERQEQRVHDASETIVTEAAEEGSSSPSPRDSTHMANGPTATAAADWAAAGSGDPDPLSRTAFFGDALIAALNDLPHETRQDLTAAAAATDGTAPYRHGRQLSPPGAGVRPGLGRGRSDRGAQSADRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYP8</accession>
    <name>A0ABQ5RYP8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="122" mass="13589" checksum="A95E67DF175D6845" modified="2025-10-08" version="1">MLRHYVDANNTNWCDLLPQLKFAYNLSVQAATPFKLVLGYEPTTLLQLPQVLPRPRAGQRPKVEGRVPPCRWTVTTPIKVTCELIRSGVDALVDSADSAGCRRWVLNLGKALISEKSAWETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYQ5</accession>
    <name>A0ABQ5RYQ5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_005498</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052207">
        <property type="entry name" value="Max-like/E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741:SF37">
        <property type="entry name" value="LD38259P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="156"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="258"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="484"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="592"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="680"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="303"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="319"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="507"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="595"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="621"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="687"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="728"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="938" mass="95842" checksum="567589AF90698B6B" modified="2025-10-08" version="1" fragment="single">GGRRRGGGAGGDSEGYEDEMTDEEARRKEPRERRVPAQCRRFYEPAIKVTFDEGMKLADGRIYVPLPFVHKNFSEQQFPLRLPADIFVNGELINEGFVVKIARSQNKTRRHGLGNHWVTGHQRLIKHYKDVRLTGLELVENNKLKIMLSASGVWDDAASSQQSQQPPPPHPQQAPPHLLQHLPGVHGMAGIPGLTHGSLMGLSPAAYAPGLLIKPDGSSGSAAAGFEHLSAILAMQAAAAAAAAATGHNPLMAPHPAMSGLGGMLHPGGATNPLHPGPVPPPPPGQGMGPATGNPNTFLAMLGGMGSGPTGGGSGGAPGTAFAPPQLTSPLLLPPPLGLSHNATPQLQQQAQPSAAAQSQQQQPGSEATVAGASNAVPAAGPSVSQQQPNDASGMTVAETQSAAAAAGGRSGGQSNPDIAADLTASPQQQHQQQEQQLHPEGHQRPDYSSLEPGSLTAALGLQDNSQAAAAAAVAAGGEGQVVAGSAGDTGDNVEDRAEGGSVAHTQQHSHPHAMQQQQQQHQQQENEDTSPDQQQQQQQQQQQQQQQQQQQQQSEDPGMSWGTMSLAPGPLPPLLLSHHAPLYPHHALLHPHQHPQQQQQQPSSAVQPLSDGQSGGGAGPSDDGGAVDPGAGGNGGTAAGASATSVDSALAVMHSLMMSASGLTHGHYGVTHGTTGHSLPGLPAISSLSGATTTPPGSQPGASPFPPVGGASPNFLLPLMQGPAGTQSGGSSGAAGGQQDGQAAAAAAAAAAAAAAAAAGHMGPLTALLPAGMMGAAGALNGDLLAAAAANGQWSMDAFQTLFGAGAHAGLLGLPRSGLIAQPPTTLSNATAGSQQQYPRLYDGRIHVPLNLINSHFANVEFPVSVHTKVVVNSDVIGESIQARIVKYKKHRRPNHFNYWITGLQKTLARYNDVRQAGVSMEPGSAVIRVNLVAASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYU3</accession>
    <name>A0ABQ5RYU3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="308"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="485"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="677"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="766"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1216"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1373"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="917"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="568"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="681"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="720"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1244"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1279"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1307"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1375"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1394" mass="140510" checksum="0BF155D28750C9BC" modified="2025-10-08" version="1" fragment="single">MSPSARPFSASSARSNTAAADGSIGTFFMDRSGFLGLEGGSKLDRPVSETVNLLIDAAHQRHENRNVRHPATHAYTLFAQRAAEVSAINTSSNSSKCRNPSSGGGGGVVPSLSLDTSRSLGSYTGHSSTAVYAWGEGPLSSRGSKGPQVPLRGANGGEGHVPITAATSAAAPAKAAATVVVGSTRTVAAGSGTTGPAGRTGRIHREPSPLGRRSPAGRTVRQTFSPMSGLKPVVATGECRKGNVTPTSTTVVISGKAPQTAKVKAAAAKPSASVTMTAVNGKSKLGSAAAATAAAAAPSSPRSLLAGATANAHSAMARTSAPLSLSPRRQPPIGSNGQEGSGSPGHIDWGDTARAAVKGEVIVEESSYEDPRTVRRVKSAGNSNTAGDSGVTATVTVAATAGACRAAEEQSRSPRQFVRSEVVGRALSTWEGADLEVVCMYDSNFQSANRQGQHGVPMRVSSRTTIGPISYLYRWYCKRATKFRSPMTPEDLPDAPMLSPGESYRSVPAGSLNRGGGGGARSGSAKRNSRPNSGMAGGGAAAAADPPAWLRQATTRRNHSGPSIVSGEGPGVATGGCGEGSRGGVAAAVTSPADHERLTTATARSGSGRRLNPCLDPLISLSELVDLLEDMEIVPQLATRVDVTRIFNAAHQAFTSATGPAGSETITSTAVISSTPANSTAWGAPGGNAPRAAAESHHSNSKPPPAQGSAADSSTTGRHPASAAKPRPASAVTTASGKPLDAWSSISSGFSINSIMKTAAAAAAADSGGPRNAAGRPSSAPRVLSKPTAAAPAAPAAAAATGGVVGSGGSCNIIPRTQPHPEEVRFPAFKDILVRLAVAIACTEPPVAAGAGVTSAAGVNSAVAAPAPALMPAASSAYFSQELTFPNPVLTAAASAVGCSSRGWGRTMSTEDAVAAVRRLLERMKLYRNDIQVLKHRLDELARLANEHAVRARDNRFRYVAVRSVVEAAQDIGVSPGAAQLPPGARPGMSSRPLPPWSVLAASPAPSYLTAILTEEDQPDFPYQPAWREFGAVALDLGVLQPGELRRCRLLLYCRGPYTLSVRIDASDAPFCDVTHMGLQSVPPGLPFRVDVDVKVYDIGEVVGELRLLYTSAQDRRERKLVVPVYGMVCPVGAAAANDVRTWARCASERSVIAAKIAREHPAKASTSVVSTVVVPPRRGPSSPPSLSASLSYLGGYGNSSTSVLACKPCTNTRLGESPSAAAVAPPSPAASQTGARRHVSSSGGDGIGPGGGVASSTGGGSGGDKPPSRADSGRSSSGTGGSANGSGSGGGQGEAGGATAAGGGCEAEVAETGGIVTGPPLSPRGSSTRGSSRECYEGGLSAAVSACAVNPASDSGLRAVGVAVPAVGGFGGAKAEEEASGRDEAESAHVEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ38</accession>
    <name>A0ABQ5RZ38_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MTD1</name>
        <name evidence="9" type="ORF">VaNZ11_105684</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-catenin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045156">
        <property type="entry name" value="Vac8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47249">
        <property type="entry name" value="VACUOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47249:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="609" mass="66013" checksum="321E5E0937852FE9" modified="2025-10-08" version="1" fragment="single">MKGVTGDRSTLCKGGLSSLPDSNIAPEAPGISRVATRRDRDVKHEPFSKQDLNLKDVACQVSSSDRQVANKAVMKLCELTVPTRCNRVANQLALVDLGGLVLLLEYMAARFRDNATNDDTDALNMALLVLENLSTNVQLHQEMICNGALLQFLLNTISKQKQRAMRVNAAKVLVNLTSSNPQLQDKVARAGSVSAASALLRESEVVLARQGAWLLSNLTASHGCATREELGRQLDVLESLRDILIRSEDRATKARVCEVVCNLAYNDAELHANLVQVDMMPALMMVIDPSVDRRQEPEVVASALLALAALVVANGGTIPHEVSQLTTHLAGILDYSNLVTRDYDMSRVMVAAHALIYSLGTYVAKDMLDVCGTGYPEHVRKLLQESGYVSFQQSLLALGLKQPPYMAASLGKDLRGPHCFMANTTHQVNIELLPLLRHDNVRMVVNACARLYQIATCLRDHPEGRLVLCNGSLIVVLQDLLDSRQSAVLQATLSLVDALAAIPELVPLLVEKSIMDLVNRLSDQETLVATTNGSSVSAGEVESTTVSVCELSTATVMQDKETREGHELGGDGIGKGSAAVVKRRDPLVKMLAERALVTMFLAQCSQIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ46</accession>
    <name>A0ABQ5RZ46_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="788"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="841"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1418"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1498"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="790"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="864"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1430"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1498"/>
            <end position="1509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1641" mass="172150" checksum="A7BA9419953D4699" modified="2025-10-08" version="1">MQTVYPSYVSNESHGLFAQTINWLRNRNRLHCPQFSWIKAASQESQATKSINYAQAGTHGFSPVKPLCRNQAPASPKEHGMRPLIDDAAYAAAPAASLEAGATTVLGGCNSPRPNCDRGLCVFGVATADGKLASWRDQTRYHYRQDPHSPLPTDILGPQNGPGIYHEHSALHSLYVSQRTRTHIEPRVSCRTVTDNPEITQLSNGVGVEMDVGVTEAEVAAADRSGATSTGSRAGPAHVRTRQSYIELTPRLRAEMPYPSSYTAAMAPTPSQALRGRKHSHATPLPLPQAGRSAAVQEAAMAREAAAAAGAASFSWDPSSSASTTPSAAKGLSASGLSAAIRAATSLDELVTLYEVQPEAFRSGHVSAALVRLPHAAGLRRTRTPRLREPRHEALLQHLLARFEVHRAAGEYGTRDLSSCAWVLGTLGRRDCGAVVSQLAAHMLALRQQYLRQREAEAAKVKVMAAEWAEGLRNEQFVAMASELGGWKVDGQPSGEMSPLLMGTTTRYDATALPDAMLAVAATAVAMPPGRASDCTPLDLSNMALGMAKAGVTSPHLWKDLAAAAAAKTLRGFGTQETANFAWSLAQAHREFRRNEVGTAPNFDRVQEAAGAGADTGASATDDRGTDHIDDSWQGFVPWPGSCGGAVVAPRAARVLHPSWLPWARDFIEEVLAPRVRCMLRFYTTQQAANAAWALAALGHADSRTLVAVAAVASVSAEGSLRAATGPVAAATAIEGRLCAATGPAANARAVTAAADRPAQNRQLLTPQGVCMFVWAMATMLSTMRSHVDGTSSISSTSSNSSGSTITNSCCSSASMCTQANSIRVGSRRNSVAHITGFNITTRLGDTPHGKGPKHSIRGSRYSLPPPPAPVPPLPGPHPEPPQLPPARPTRPSTAIHPRALLALTRVASAHITRFSAQGLSNLMWGLGVLGFQSPRELRQLAAREALLRLTELKPLGMATLLEDWARARRHHPELFAAAAPMVTAKLYAGGCSPGSDIQPRTLTRLLRAYGAVGHVNRRLFQAAAAALVPMRATCIGGVGGTLQGAAVSAPLDELDPGELYQLVCAYGRVGVHAPRLMDEVLARLAPVLTAMSRGTGAGDGGSGGSDIGPPPRAAGRLLCGLAVLGHRPAAIVMHGAHVDGDYTIGSATAVAAMAAFRALRRSVLTGICVMTSRHALAAIWALLRLRAAGAPEVLQMSQLLVRRAAPAADSLAVGLAAVARTRAVSGNPVLLTRFVWVLGRGAAAADADAERLRATDTDAATAEAEAASAVFVELSGGLLAVAAALLGKEDKTDRMGGVSLHGDVLVALSEAIAAILARKTRAHATVDKGASPPAMLQLYDRLYTCFLILVGLALHRSGTTAAPQQSSGQLTARALADLVHAVYDSGVAATHPAVRVAAARQVLFRLQAVSLHNTGMATGPGLSAHRGRSYTTQPQRPNMQSRRHQQLPPRLSVEELARLLAAAARMELFDHPRAQPMLREAARALMAQTAPSGMGIATDDGGAATSDGGADGGQYPHTAPLSPVGLASLQELIRHCWLPDDGNMENCVNDASSSSGGGSGCNGRQSIAQDARFANISNVPNLRGGGAGSAVAMAAAISSGRTLRRTLYRWAKHEAQPLLPWLCPETRALVLKASRADGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S033</accession>
    <name>A0ABQ5S033_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006064</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF155">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="57"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="261"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="400"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="711" mass="77297" checksum="E61B5D83191D5419" modified="2025-10-08" version="1">MSKEPSRKRGMLSLEPTDHQGTAKRPAVTEAARNRALAMLKSTYGFETFRGKQEEAVLAALAGRDTLVIMPTGGGKTICYVIPALMQPGVCIVVSPLLALMQDQVANLTKVGVRAAQLSSALPVAQRAAVLADLTGTDGGDQPTIRLLLVTPELLAASGRFRSVLRQLHTRRNLSLFAIDESHCISSWGHDFRPTYRQLQVLRRDFPGVPIMALTATATSQVQDDICEQLRLHNPLRLLTSFNRPNISYAVRYLDVEELSEVEALVKLLRERLTSGGTVPCSIVYCQKRETCEEVAEKLSREGLPAKAYHGQMNTHVKKEVLRQWQADEVPVVVATIAFGMGIDKAGVRLVVHFNLPRSLEGYYQEAGRGGRDGLPAESVLFYSRQARKRMDYVLEQDARKKAATKGRRKGSAAAARTGMGGDEEDEGEEGEEGGKGGAGGGGGSDEPDVKDAVVTAVSDKEVPADNLAWKAFGQVVDWVLTADCRRNALLAHFGERSPGYGPCGPTGAGHAAGCDYCRDPQHVREQLAAQKQKDLEITVRSQGSGKGDDEETNMFAVRGGCGGGDYAVRGSGGGGDGNDDEEGPGIYHPPLVPLQAVRSIKNTFLSLPPVRGSMSDECGGLEATSWIGCLRWHQGALLEAAAAAAPLRRRHRRTCPFVRQPSASWRRRWRRDRRGAGWRPPCCNSWRRPSRLECMATVCHASSTLAAWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S168</accession>
    <name>A0ABQ5S168_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_006466</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="9">
        <location>
            <begin position="102"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="10">
        <location>
            <begin position="288"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="10">
        <location>
            <begin position="405"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="11">
        <location>
            <begin position="826"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="729"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="906"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="941"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="729"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="753"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="767"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="909"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="949"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI63485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1211" mass="131029" checksum="454836E471E31B89" modified="2025-10-08" version="1">MTNAYTAAEHTNYHFDVNWESLCPALDRFAQFFISPTISRDGIEREVKAVDSEHSKNLQSDAWRKSQVNKATANSKHPWSRFSTGTYETLYTLPLAAGTDPRDAVADFYDRYYSADRSSLAVLGRQSLEELEEMVRPIFAPIPKKQLQRSGFTEDVFLPGQVGVLIKLVPVREGQSLELTWQVPPSERLYREQPLGYLSHLLGHEGDGSVFALLKAAGWASALWAGESGGSMSFTSFFTIHIDLTDEGQRNIREVATTVFRYLELLRRPGGICERIWEEVRGLAQLHFDSRDRSKPLTYCTTLSSGLQLYPAKDLLPAVYGVPREFSPSNIREALELMTPQRVRALWISKAHNPPAAAPTEVASADAQPMAAQEAASLDEAEARADTAAAEAAEAGVVDTTLKEQDQELLTEPVYGTRYNIGPLPAEWLAEWIAACSQDEPRLHLPAPNRFIPTDLSLADDESNAAGAVPTVAVAVPGRLRIWHKPETRFRQPKAVLYLDVQSPEAYTSPRAAVMTRLFTKLMLDYLNEVAYPAQQAGLDYNLLNTQSGWHLLLSGYNHKMPELLEEVLERLAGFKVLPDRFEFVREGLVREYANQLHNQPYSWAMYRAEMLTTARRWPIEVYGSEAATVTAEELQVFVRHRLCSRCFVEGLGAGNLRRREVLHCAALLLNCLRDKCGAAELHSAERAELRMLEIPPTPLPPLASPPTAAVSAAAAAATAAAGASRDATADAAESSSGTSAVDVGTDDGGGAEAASRDAANAAKAADGNKGKRGNGEGEREAVTGISGGWLFSEDVPNPQDENSAALVMYQVGPDDLRRNALRSLFVHLAKRDAFSELRTRQQLGYIVSLHGGSEHGVGYVEMLVQTNAFNAEELCHRLDDFMMWFLETGLPEKCGITATAATAAATASQASRPVQSGSDSGREKDMVAAADGVDWAAAGGQREKTLQTSTAAASSSSVSSGADTGANAADVSNGSDVVTAVTATADKRADSPTQFAIAVEELAKSKLETPKKLGDLANRWWSEVHKGHYCFDRQEAEVAQLRSLTPGELLSFARELLIGTPITAPSMPRPVPLSPPRPPVTTSAMDHTGCGTYHCKRLSVHIRGKGEATRAAAAVVAAAAAVPTAAAADASCGGNPRCRRPYSLIPEKDPFSWKRGCVQLPSVQALWAARRATAAAAGYNGNGSGGAAAGCEFIPYLNVAQDELEQLLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1N9</accession>
    <name>A0ABQ5S1N9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006862</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045676497" evidence="2">
        <location>
            <begin position="30"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="481"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="804" mass="79573" checksum="531E2F627DAEC4A4" modified="2025-10-08" version="1">MPLPRPAAVGCNFLVTTLISLYIACPISGAKAPSTALTVITPPDETPVIGATAAGTATAAGTATAAGTATAAGTATAAAAATAAAAATAAAAATTGTGDTVSHPPVARRNAVESAALNPADGLANNRLGGGNGGGDEAGGTATGQLAASRRIFTRSSMSMYGGMGLARGWVSSHMRSHGRRLFLNEEPKIPSAPTDNQLLLSSTTDVRNSDGDGSVGVDGREGGEGNGGDDDMDIDDGDSVDTAGGGDDGGIGIDGGKGVDGGDNIIDVDGDKLNPHADADADADDSGSVRSQQAFAVESDRSTVEDAGGDAGGDVDVDGDGDEYGDTANVDDAEGNAKNGADAEAGRAAGGGDSNDSDGDDGGGGDGGKDGGSTGADDSDGLLDTRSSGRRPDSSVDISAAAAAAAAAAAALNLGAPAAVPAILAAETRSGTSQQGVADVPSYGGDREDWRRRKHLPDPTKLARELAGEEDDGASDTMRAAAAVAAAAAAGGSKSRDSGAKTSSADSGGGGGGSKKGVERDRRRRRDNSGSKDVCAITAAGAPSGDGGTSGSEVASPTAATAQAEYSPLFATGVCDGRGKTTCVASGLDGKGIGGEDFAPNTLYGECPDGLPDIHGYVSVVEVTTVMPERVLRIGEPAVIRTAVVCSVIPVVSVVDIFYTNDASMTPNRTWVLLSSVPCIPSTDFNGQPAVTNITSASFVIPPGVLDTTLVARVQLREVIPGTQLISAAARSDTAPCSNEIYGSSSDRDDVVVRVCPALPGTEGLRATDGRQTGTGVEVRASGTVRLRRPIWKLLAARRAAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1Y4</accession>
    <name>A0ABQ5S1Y4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein phosphatase 1 regulatory subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006968</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SDS22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF23">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="35320" checksum="D39734CDDF32A2A4" modified="2025-10-08" version="1">MLTELDLTNYHLPDLTGVEIEPSLTAIDLTANRLRTLDPRILQLQGLRCINFRQNLLANVSAWSTCECKGTLEDLEFRDNHLSTIPCLKGFVHLQRLDCSYNQIRNLVPLADLDSNRLEELYVANNKVTAITALSHLTALTLLELGSNRIRMIEGIASLTCLRELWLGRNRITKIDGLTTLVNLRRMSLQSNRLTSMLGLESCAALEELYLSHNGITTLEGLAPLTRLKILDVSSNRLTTVEVSALATLTQLEDLWLNDNRIPAIDVALDRALDPVRHSLACIYLEGNPAASDPQYKRKLVNMLPKLKQLDANFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2E3</accession>
    <name>A0ABQ5S2E3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translation initiation factor IF-2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_006468</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03692">
        <property type="entry name" value="mtIF2_IVc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF5">
        <property type="entry name" value="TR-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="554"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="38"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="422"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="179"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI63487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1077" mass="112112" checksum="1DA8F6F7BA749BA5" modified="2025-10-08" version="1">MAAYTSLPSASGCSSLQPIKAPLGFRYCPLKWRTGIIAAAGPGSAGPQPLTKPPLSPPRPVAPPPKPPAQFPAQQRQQPASSNGNGNGNGNGHANGVPAPPRPSPPPRPGPPPAAPKPSAVPLSAPPGPAGTAVRPRTGTPPSGPATLPPQGPVPPSALRPGPGPASQNGSGNSIMSAPPRPAPPPPPRPPPPPSAGNRPPIQPAAKTIASTPRPAAPVQPTQQQPVANPFANISRPTSPSPAMAATGMASATPVANPFVNISRPAPPAAATPAPPSVGLAPPPLQRPPTPAPAGAAAPPNLSRPMSRPKPSLPPRPLGPQSAAVPTAAPRPPGVMPPPARPDLLPRQPPATAPTTAVAGTGSLALQRPDPPSLRVLEPLMGPGAQAAARRLEVQEVEEVVEEEGGELFDDDDLEFMNKGAAMKGKGKKGAGKRKEKVTAEMKREARRQREVVRMEKEASRRRDKEEIFEVGDEGMSLDDLAALLQVAASDIVRSLFMRGITLSMSQMLDKNTVKLVASDFDVVVVDKDPVAVTNAAKKRTEFISEEDVDDLAPRPPVVTVMGHVDHGKTSLLDYIRKARVAAGEAGGITQAIGAYNTEVEVDGSLRTICFLDTPGHEAFSAMRARGAQVTDIAIIIVAADDGVRPQTREAVAHAQAAGVPIVVAINKMDKPGADVDRAKQELLELNLVPEEWGGSTPMVPVSAKKGTGVQDLLTQVLWVAEEKQLMSNPKRPAAGTVIEAYLDKKKGPVATLLVQVGTLRPGDIIRAGASYGKVRALSNDLGQSIPSAGPSIAVQLTGLNSVPAAGEEFEVCVTESAAREAAVEFEDKIKLRRMLEMSGGGSLVTLSSLATVDEDQEAMQRLNLIIKADTMGMVEAIRAALSMLPQQSVLLRFLLAGAGEITASDVDLAAASGGLVLAFNLEPDEAVSAQAKRMGVSVKSYRIIYELIEDVKAAMEGKLKLVEERVPQGTAVVKAVFGSGKKKVAGCAVTEGKIAKAGHVTVKRGKTMLYEGKLSSLRRVKDVVEEVAAGLECGLGCDGFTEWAEGDIIECYLLVTKSRRLEDARATTAVDVSTLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2W9</accession>
    <name>A0ABQ5S2W9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19094">
        <property type="entry name" value="AKR_Tas-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF4">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="60"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="400" mass="44962" checksum="275BFB18ADACC796" modified="2025-10-08" version="1">MLLTGRQVFDNGLRQASRSHRGAHVVRATALPLSSETKQRLKAADRVQLGESDLLVSACCLGTMTWGKQNTEAEAHEQLSYAWDQGINFLDTAEMYPVPTEATTQGRTDRYIGSWIKNSGRKREDVVVATKVSGYTDRSVWVRTPPRTVRVNPEQIVESVDASLKRLQLDHIDLLQIHWPDRYVPLFGMGPYDPKNERPDTVPFEEQLRGLEEVIKAGKVRYIGVSNETSLGVSQFCHIAKATGLPKVQTIQNAYSLLVRVPFETDLSETCRHHRVSLLAYSPLAGGMLSAKYNRLSDEALKAARFNLFPGYMARYKASLVQEAVDKYADVAKKYGLTSVQMALAWCKSRWFVSSTIIGATTLEQLRENLLSFDKELSEECIADIQALYRVYKDPTVNPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S321</accession>
    <name>A0ABQ5S321_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_007532</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04842">
        <property type="entry name" value="Peptidases_S8_Kp43_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051048">
        <property type="entry name" value="Peptidase_S8/S53_subtilisin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034058">
        <property type="entry name" value="TagA/B/C/D_pept_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399:SF4">
        <property type="entry name" value="CELL WALL-ASSOCIATED PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399">
        <property type="entry name" value="SUBTILISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046537323" description="Peptidase S8/S53 domain-containing protein" evidence="7">
        <location>
            <begin position="22"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="300"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="9">
        <location>
            <begin position="563"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="964"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="964"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI64305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1129" mass="120887" checksum="53FDA075D3EFB653" modified="2025-10-08" version="1">MSYLVLFIVGAIALLSTKAIATKEVPLRTGTAVLMPPDPEVRPTKIEIDVQIFPPYAWGSLPASELYLISYENDTQPELIQQNITMVNGRIVSYFPDNTLLVFAKAEDVWALALSLNTSMALYGPETKVAPELPDLLQSDNLQDSVITRVSSNDTVSFNITLQYGLLVELVPTVTPLMMKYAGQCWPRELVKKGLGKSYSKRHPCWPVTVPHSASVTLAMYLCKEDLEAGISWLASLTVNRAIYPMLQYGFMNLLSGSLLQTDRLGLTEYGWTKDPDGGQKQFELTKDESPYWSAGLQGQGEIAGLGDSGVDVDQCYLQDPKYQWFRIASLTGLPDAPTIGNMLYWRMPEHRKIVQYAFNPASGDAFDYHGHGTMCAGSIGGAMLMNDSDPFSAVRQTLATGVAPRARLSVVDFMQASASGLSVPSAIDIDYLPVHAAANATLSSDSWGSLLGGYNAKARGFDMYLWKNPDFISFIAAGNYGERAPANDTIASPSIAKNVVTVGAGYRVPRSAGITAGVYIIRAGENIDFLQINRTLVLKESPNMPLLASVIPSGTFVEVAVADPITACAPLNNAAQVAGRVVLVRRSSYCSIFDQGLNVLAAKGIAVIVVQSTADKPYGPSLTDIEVSQYPAQIPISMTYKYFGDALLDYLTSSGRGRLFVTGVVVPVDVDTVAEFSSWGPTYRDGRIKPDIIAPGKDIISAMTSLRFGSLCDYDTYWGTSAATPLVAGTVIIMRQYLRTGFYPVGASNNILSTPFTPSGMLLKALLIAGAKSLEGSLAMASGSLMGPPPDGYQGWGRLSMSGSLPLEGFTDPRVRLQVLDRGQFSLPGQSVTVSGLVATGTGPISIVLAYYDYPADVNADTALVNNLNLIVSVDGRNYTGNNDENNAMPVKDSVNTVEKVLLKTLPPGANVSVVVEAPSLPSLILDPTTPQRWAVAVVGHFSGYLESELNPFWAKWGRGMPPSPPPTVPPRPPRPPPSPAPAPAPPRPPPAPPSPRPPPRPPKPPRLPPSPPTPPSAPPAPPPLNPRPPPPPPFRPTSPYPPKPSLPDVPMPPSLPGTPRAPSPPSPQPTPPLSPPPSPAPSPTPSPKSSPRPPSPIKPPPTPRRPPTRPPSPPRRPPNRLPTPMQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S330</accession>
    <name>A0ABQ5S330_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial processing peptidase alpha subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007013</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF49">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="3">
        <location>
            <begin position="109"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="4">
        <location>
            <begin position="260"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="56469" checksum="AD47D47D909AC8C5" modified="2025-10-08" version="1">MYFPVLESISGKAICSVGLRRLLSGGIPSGGHRCIKTCAALTAGDSIVGGLSSDVSRVSHDFLGWLTNGGKRITTPLSQPLPGIRAEPPGLTPLAPPPTQITVLNNGVRIISEASPGPTASLGVYINSGSIYETPHNSGCSALLECLGFKSTTHRPTLRIMKEVEKFGNTIVANASREQVSYTIDCLKTGFPAALELLLDCVLNPAFEEQEVEDQKLRLAMLLGGKDIHVTLMTELLTRAAYRGPYGNPLIPDPDSMTRITPDVMRSFVARHFIAPHMVLAAAGVEHGELVTLAKPMLEGLPKLAPLPEPKPEYVGGFVHLPGSYAQSNLLLSFEYSGGWRDVHGAVVMTVLNYLLGGGNSFSSGGPGKGMHSRLYTRVLNKYGFVHSCASFNSTFNESGLVGIQAASDPNRVAEMLNIMCSELEAVENGTNRMELERAKRSAVSVICNALESKATSAEDIGRQYLTYGHRISGRTYVEMLEAVTQDDIRRFVRRLLSSKPSLAAYGDRTETIDPYILSRRYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3D2</accession>
    <name>A0ABQ5S3D2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_007610</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF1">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="8">
        <location>
            <begin position="338"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="535" mass="57965" checksum="EAE61734A7CF843C" modified="2025-10-08" version="1">MSRSSCMLSSHRFSPRLSNNTPFRTTVRCSKAYHVGPFPAYNPAERHVFNRPETRCHGVKRAAAETLVAAAAAAPVTAPPHTQDPQLILKAMLAPVASFFGGESSWQFYLAQRFFIEVVVVAATALLGYWLLTLLAKRSAEHHEGGGGAGAHSGGRHSGGGGGGSHSSSSGLGGGEVLFMAADALTTPMARVLLTSVLAIHAARSTTYIFDGFITAFNPRLPNDWLDDLVRLMVQSLTPLDGLLTKMSLVCLVLCSCAVLLRWKDILLSHYLLPHVEAMPKGPELVANFFNPASNILNWIVIVASVVWLAMALGINLKPVLAVGGASGIIIGLATQQVLSNFVSGLNIFLARPFVAGEYISLISQNLTSQTNVSGRVIRVDPMRTLIATEDNSTITVPNQVIAISIVINRTRSPHWSVGKPSSVLANPRELHWRMRLPHSALDNLQEVEARIEETLQHALPQDKVRHSTTKLMVVKFLESGVEFTAKVHLAWRLGAVSAPPALERKEKQEELIQNAMLSLQKVVRSFDGTFTIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3T9</accession>
    <name>A0ABQ5S3T9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="196" mass="20921" checksum="CDCD3C68F7555C53" modified="2025-10-08" version="1" fragment="single">GIGFNLQLSSVRLVAMGLTASEEDLDLTPQQLGTTAFKLPVFNIETIQAAPQEKYIMKLRSTSMLGDLKSICQELAGTLPGSGSRFRGACDPITLPAVFNFSRPDTAPLPWAFQSFTVNSQVDLDQMRSLLGSSLEYLERDLEASIGVVNTTANSSPSESANPTSTHNGSSSSSKPVPRKDCACAERYVPVCGIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4J9</accession>
    <name>A0ABQ5S4J9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNL-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_008135</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024158">
        <property type="entry name" value="Mt_import_TIM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007853">
        <property type="entry name" value="Znf_DNL-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922:SF13">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05180">
        <property type="entry name" value="zf-DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51501">
        <property type="entry name" value="ZF_DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNL-type" evidence="5">
        <location>
            <begin position="117"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="181" mass="20483" checksum="F678C60B3C0396B5" modified="2025-10-08" version="1">MLARKSPPCSGTLIGTREWPVRRVLAANPIHHQSPNLPAILKKCQGCIPWMVQRVRNWSQRRRVLADSPLVCHSYAMEPVPADLRIEAKEEDGTSDIEYDEQNKVVRIPLSALGPEGQRRTKLVLFTCNKCGGRTARLVNPIAWDKGAVFAQCAKCSVWHVLSAPNKKIFEEVRYNQEDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4U2</accession>
    <name>A0ABQ5S4U2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="362" mass="37151" checksum="A188E976211916A8" modified="2025-10-08" version="1">MTGLTAHIAQPSYGVLQPLRKQLQQPVMSMQAAAAAAAAAGIEPSLTVGRSTNVSSATCTTGAWPSSEDEGYEAILRSSLGSEGEGSVLNKSLRSLALTRYLCGKNPGDPGRSPEVGDAPPWAPPWDNLPPALDVVPRSYEGHNRGGFGGPASLSYGSEFEVMDSKGGGGDRNVAAGPPLGCTDGAGKEGGDGRGEGRDRRGAGVKEGARPPTTAEGTAGADDAGLDVGPSGCSPRMLHGGRLDLRVFVADSVRLAHRTAKSTSAGQRAHAERHWSLLQHAVQCGWPALVRLLLGLHERCTDGDVSDVSLLSGWQLEQLRSTPDVLVPADAALLHLAIGSGVPAVVDAVVTAVQLYGVSGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4Y3</accession>
    <name>A0ABQ5S4Y3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_008299</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="81"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="84"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="128"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="128"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="182"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="7">
        <location>
            <begin position="185"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="188"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="233"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="295"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="328" mass="37014" checksum="39918F4E07BEECC4" modified="2025-10-08" version="1" fragment="single">GVGLAAEQAAKVASTSNDMQFLQEQLKRIATVRPDSQEAIPIILRLDDEAWARVAASHRVGRSGTECAAYYRHNLRPGLQWPLQESQKLMELAEKYGNRHWSQVAAELGTGRTAGQCLSHCLRWSRFNRRPQRGCWNEADDNMLKALVAKYGTDWVAVAEGFGGRFDRHQVRDRWHGVMKANGPRRVGKWTPEEDAKLTQAVDQHGQKWTEIASHVAGRTARQCRERYVNLLRPGLKFGAFSSEEQQVLIAACKELQAAYGRIIWARVAERLPGRTDDQCSRAYEGLVKHDKAHIGHKRGAANHLLRGTPGTGSRKRPGKRKRIEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4Z0</accession>
    <name>A0ABQ5S4Z0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GRIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007967</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349:SF6">
        <property type="entry name" value="MYOSIN-6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0518">Myosin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="171"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="240"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="457"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="658"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="729"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1676"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1733"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="70"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="547"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="823"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="974"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="267"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="500"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="666"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="756"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1744"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1756"/>
            <end position="1774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1819" mass="185297" checksum="9F8B1A6E08A10D75" modified="2025-10-08" version="1">MDPFKPPRKSPAKRGNLPMGVGHIAAPQTYKQAGPPQSGRKPQGLLQTLNKLEQESEDRAFELIRLRTLVLKLEKEKEDSATVCQDLKRENAELQQELSKSSTELQRLKREHNELRCRQWLDNAQGLSHVRAAAMEAAAAAAAATSMPLAPGGPSGTVAASIHGRSVVAGGGDAGGGGGGGGLNATAGTTDMLLTTADDPGPPSPRSDTLLTATPPAAPAVAGESLLQQPGGHSLTAIEESREGEEDEDGGVGGSSDVAAPAMTPRSKSLASASALQSGPQLSGGAAATADGGLSEGNPALVRTCNSTGSNNHIPRCNSARGHQVMCASGQPVGITPPMSPAIGIRATLLAHNNTAFANSAAAASAASGGIGAAPASVSPSLPSQQVHQQKATVAATVLLQEENKLLAMQLSDFKERVMYLQQELAEEKIKHDASIAAKRELEERLTEITTAAIGVPHHGGVASRGGWRNSSGGAPGSGGARDLQRMQSHQHSPVRVGGANPGGGDGGGGDGGRGDRSGLISSSTEHSLGALQDLISKLKEDRETVLVAKQKELDAVKQRLDAANKEVEALKQQVSEANRVIVEKLARAPSAEDVAELKQHIDKLKRQLHSKNKHAEEANARVASLMRELAMRDAAAAAAAEAAAAATADAIAAAAATPPVPAPATGPSSPSRPSTSDVTGSPGGSVSSSSVGGATVMAAAASVQLTLPLPPPSVSQTPRGSAVELNPQARAATADSKGTEVSTSFLIVPTESSEARLGSARPGSSSRPASSRPESALSPQLGAAPLAQAATGAIVRRLTSASVASADGLRAAAAAAAAAVAAEAAAAKLTAAEARVRDLEARLKELESQTVAMAVEQRRLEDEAAAAEQERVAREARLAEHAATEAELQVRCVTLTQQVEQATARGAEHEARLSEVRVRVSELESELAAQRAGADAKAAELLRQLELERHEVATGSAALAEAQSALAATSLELQHRNRDLEDIRGELADLRQQLAAAREEAAASAARAAEELTESRSATEVAEAAAEVARASAAAAEADVARLEGSLDAARAESADLRGRLEAAMGRASELQTRVAAMEASCEAQVAALAQHDTAIAELHGLMHDLTERLSGVTSERDGAREELAVAREAAALREGELGTARRELESTRAELEAKSAELADKATHLATSLEQLAAVEPSLTSMRSSMEANKARAAELEAHLAASASRADSLAKDLGEATARVSDLAGQLSTAESRGAQLGRALASKDMEVASLHATVESQAAELDDLGARNARMQGLIKEMAGQMETLQTNLSKQTGLVAKLEAALVAAGMQTAEIEELHRSQSLVYRERIEDSQRQLSSARLKLTEMSKEVNRITARGGVASGALLASARSELAALFKKMKHVAQAYRLTLPTIVAKQLRTDGANLTELQLFHLLHAVLAALEATGGVTVSVTPSASSPPPTAPLPLMPPPVPLPLPPPPLVATQAAVVAASHPVLVAPPPINGRPGSNGVAPTPLPANYDVRAALLGQPQASLPAGEFEPTALSASLQRSASGLQSIVTGGGVGGSASGLRSPNKSISVPHLVGIVSPTMSAYLPASNTPTHSLGGANGGDLSPVRGGPYGGRYRGGAGEGMRVSGSGGGGGGTLTVPSVIGTIRSSAGGLNVGGGGGVMHSRAFTDTGSILSTHSRRASVAESAVEDGYTDRRASVGANDPYDSCESLPELRLVNSNSAAAAMTAGPVVVVAAAAAAASSGGMRSRESSRPSSVQSRPGSSALGGGVTSGGGRPHSGGSGRRYSDSGAHGNGGSGGLSNIPSVPHHLPPLVARPSSTALITVDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S508</accession>
    <name>A0ABQ5S508_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase ESCO acetyl-transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007983</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028009">
        <property type="entry name" value="ESCO_Acetyltransf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13880">
        <property type="entry name" value="Acetyltransf_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase ESCO acetyl-transferase" evidence="2">
        <location>
            <begin position="50"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="103" mass="10480" checksum="123887BBBF38C36B" modified="2025-10-08" version="1" fragment="single">GEKKASEPAQAPPVAGSASSRHAAGPALLPASSSGAAITDRSAQRGECVRVALGVRSLWVEVSWRRRGVATRLLDAARCCMVPGVAAERCGVAFSACATTVMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S575</accession>
    <name>A0ABQ5S575_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008457</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="457"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1311"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1448" mass="161268" checksum="BEB97151283F4912" modified="2025-10-08" version="1" fragment="single">ILIRQGLASALAPKLATPQHTCTRGRFTDCILHWGGHRLHICNIYLPPGTTSMAAETRRQLFENALAATTTQAMEDNRDTTLIWAGDFNFVENPEWDSSAGRDGRQADRPITEMRQRAGPQMVDTHRYLHPRRRDFTHLYHAPTPGGSRLDRIYINEAKLPYLCHTAISFHTASDHRLATLHLLPCKNEVKGPGIKRIRMAFTDHEDLATSMAEWIRNQCQEAPSVEENPGNLIAWWPGFKRRLTFLAHQLNKVAQQRRQQPGAHTQEAYLGVQQAQQALDTMPHSPHLLQQLIDAQARLARVHIEDNARVAKKTRVHWLHAGERPGPALTTFLQTQIQDQQTAPVALQAHSGRLVTNPAQLPQVVADFWAGVCRAPDFTTPEDRQSVLTQLAAANIRLPEEADEVAGSPTIAVTELGFALKKSQPGKAPGWDGIPTDLYRKFWPEIGHTMAALFTAIGKTGQVPTGFLDGIIKVLYKKGDPKQPGNYRPITLLCSDYRLLAKVLANRLGPALGRIISPEQSAFLPKRLIGASVLFLRHLPHLLRKLNRQGLLAFLDFAKAYDTVDRNFLMGAMEAMGAGPQLCSWTRILLSNTRARAMVNGFKSRLVKMEAGVRQGCPLAPLLYLFVAQALFSWLKSQEIGLRLLPSDEDRVTACQFADDAEVALEGEESVPAFLEAMDTFARASGQRLNLDKMEILPIGAVDDHHQGSTINGLKVVTTATALNLPMDNQLQPPQPDWPEMLALAYTRMQRLAHYPLSAFGRAAAVSNYCLQTVTWHMEHGGPPPDTTLTYIERLAARLVDRKQGPEDQGQRFTGVPSRLLVGHPSTGGFGLLPLRQHIRARQAMWAVRFIRGALMARRERERVMLLDTAMNTVAEEEEEPVTSGNVRSLHPWIRTLATYLEITHPAFKPWALTTAQATGPWLGGETLPEEISRIVGALGHLPPLEDVSNPPLAPGDWCWAAALWGNPLLPNNSDNGGSINGNNHRPGLETRHPLMASCRKLLTVGDLVRVYDMLEHDQIPLPQYMTWLDWTQQFLDPSASPTQLRDKESTRMALSALCNDISPAWIAAARKVVNRLQPQQPDRVEDRMQQQMPAPPDRTTVMAMIVRRLGWRLPGDSGIVPLYNLTVSQATQLQGVSTAEERLELLSAFVQEAQGMPTDGPAPPCPVTNIQRFQATLDRLWTAVRWENKNKETLWRLVVDGIPIPGNTHLSRVPIKPCGCGSYGADTDQPCSPRLHHFWECPIAKAVREQIDRHLPLPTSATIEGGLTRQQLWLVQAPPGCEQAPWDVIALAALSSMEYGRHFLRAATREHRPQWPSTHRRRQQQQTHTAEQGPRQRAITDFFNRSGGFPQEEEEDANTHPIITTTQPAMDTPTQTATSPLDRAQARTVADFWARLHSFAALGIPHYGWDQVGPQHPILAIVGTQMRCADPVDITNHGAARTEQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5S9</accession>
    <name>A0ABQ5S5S9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="7">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_008714</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GcvP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003346">
        <property type="entry name" value="PRK04366.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="407"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="712"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI65230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="897" mass="96666" checksum="A45DC219713054FD" modified="2025-10-08" version="1">MASKNKMYKSFLGMGYYDVHVPPVILRNVLENPGWYTQYTPYQAEIAQGRLESLLNFQTLVCDLTGMAISNASLLDEATAAAEAMTMCSAVARGKKPKFLVSSKCHPQTIAVCKTRAEGLGLEAVVVDEDKFVYGKDVCGVLVQYPATDGTVSDYKSLVAAAHAANVKVCVSTDLLALTLLTPPGEWGADIVVGSAQRFGVPMGYGGPHAAFLACHDEFKRLMPGRIIGLSIDAQGKPALRMAMQTREQHIRRDKATSNICTAQALLANMAALYAVYHGPEGLKTIARRVNGLASVLAAGAAKLGHGVPSAPFFDTVTVTVKDGNADKYVKLALNEKINIRKISTNAVSIAFDETSSLADVDALLRVLNCGRDAPFGAAALAPAVEGGVGPFARRSAFLQQPIFNTYHNEHDMLRYLKRLENKDLSLAHSMIPLGSCTMKLNATTEMMPVTWPELANLHPYCPPDQAEGYSEMFRDLAAQLCSITGFDAVSLQPNSGASGEYAGLMAIRAYHLARGDAHRNICIIPVSAHGTNPASAVMAGMKIVTVSTDAHGNVNIAELKQKAEQHSKNLAALMITYPSTHGVYEEGVDEICRIIHQHGGQVYMDGANMNAQVGLTAPGIIGADVCHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPYLPTHPVVPTGALPTRPSDPQPFGTMAAAPFGSSLILPISYAYISMMGSEGLKTASKLAILKANYMAKRLASYYPVLFTGPNGTCAHEFILDLRPLKETAGIEAEDVAKRLMDYGFHAPTMSWPVPGTLMIEPTESESKDELDRFCEAMISIREEIREIEQGKADKENNILKHAPHAPSVVLADKWERPYTRERAAFPAPWVRQAKFWPTVSRVDNVYGDRNLITRWATVESAEPVAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5V1</accession>
    <name>A0ABQ5S5V1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_008769</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="433"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="69"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="591"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="594"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="617" mass="63812" checksum="DCA832F1F11708EB" modified="2025-10-08" version="1">MTIGSCDDQLFARVLALLGGDTVAAKVVCSASDVGTLGADGAVTRWAVGQLRTTQGSLEQVQHGLNVSFVLSSAYQVFVMQLGFALFAAGVVRPKNIVSIFIKNFFDTCIAGIAFYLVGYAFIFGSKDGKGNGFIGNWDFALSYTDGGPGRSAPLPWHLFIWNWSFCSAATTILSGSIAERGTFVSYTIYAAVMPAWVYPVVAHWLWSPQGWLSVRNKEDRILGIGAIDYAGSGVVHLVGGTAALVGSILVGPRAGRFEGGGMSGHLHRATAAPQLYLIGTLLLWFGWYGFNPGSRLAIADDNSAMVVGRTAVTTTLSACAGALTGLLFSYSRYKVWDLLSTCMGALAGLVSVTSGCSVIEPWAAIICGVVGAFACEAGDGILERLKIDDPVSAFPLHGFGGAWGLIYTGLMAKDVYIQQVYGVALGGHRMGIFYGGHAQLLLCQVIALIVVVGWTLVNMVLLFWGLKVARMLRVTAEKEAAGLDFAACGQFNAQGACHVHSSKVNGAKGAEVLLSALSIERRGNGYLGFDTSLRSSSANPSARGAMAVRITGNSSPSATTPTAAASAAASVLIAAAAGDGGIVGDAGCGGGAAAAAAADGPDAPWAEQSRSPNGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5V3</accession>
    <name>A0ABQ5S5V3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab3-GAP regulatory subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008077</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026059">
        <property type="entry name" value="Rab3GAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032839">
        <property type="entry name" value="RAB3GAP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12472:SF0">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12472">
        <property type="entry name" value="RAB3-GAP REGULATORY DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14655">
        <property type="entry name" value="RAB3GAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab3-GAP regulatory subunit N-terminal" evidence="2">
        <location>
            <begin position="461"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="607"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="538"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="807" mass="85464" checksum="B9E5FF215B0BC918" modified="2025-10-08" version="1">MGPLSSVFTRTDLASLLPGDRFCASAPCFRRRPLKPMFSGKMHPKRTLPGMSKMIKWSKHFETGERPMVPPGPVMKPGQAKRLRALPAQLPAVRQQFLKMTLPRWPGWPSPGRHRRRQALNPMVLQGEVASASAAETALAVPSAAVREAHKVMMEDQELLQQEQQQRLIAAAATPAQPPSKYSLVVLLSGTREGALQLHHADTGAVLLRQQLHSGPVVSIAVRTWRMGLNPSDAVEDVTVGWADAVVRLAAWELRAAATTCYAAYAAAAAVRARGVAGAPGGGQSRFTLWGSSPAAAPTGPEVRPLAVQKWEMPSGAARGRSCVVCCGQRPRDLYSLLQGIGSSSIATGKTIFLAAGKPPSCLAALEVDEQSSGGGGALSYISSVATSVWGLARTARSVAAAPLFLRDGLKSFVLNPLQLAGIGGGSGGVAPSAPAPLASFPDAAAGQRPPVTGVWRQHRDDGRAVLSLAPAPYGSLVAAVDNLGRVFLVELGTMLVSRMWKGYRDAQIAWIEVHERNVARLLGRQRSEDRCDRKPAQQQEQVTEQQQQQQSLQQGRKQLQQKPQHTSQLQEEPPQASASGPSLRGCDGGHNDNACQQVAIGPGAGCRDADRSGYKGSHVQDLDRMAPNKGERDRRPNYRETVNPGSHAAQSSSMFSLPQEMAGQPKAAPGQGDAQKHVCEAAGGHGGRSNAGEYMLLLAVYAPRRQTLELWFPLQGERLAAIASPPKARLLQRGGGGAGGASGELGTWRVADAASAASPPMTPTGCMMCFVGTAPNLGSGFELEQAASVGRDGLQLLDVETVFGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S631</accession>
    <name>A0ABQ5S631_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_008577</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033865">
        <property type="entry name" value="Ataxin-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006155">
        <property type="entry name" value="Josephin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159:SF0">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02099">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01233">
        <property type="entry name" value="JOSEPHIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01246">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50957">
        <property type="entry name" value="JOSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Josephin" evidence="14">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="13">
        <location>
            <begin position="365"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="207"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="253"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="326"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="282"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI65124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="446" mass="46996" checksum="B14EB16B736DB98F" modified="2025-10-08" version="1">MLYHEKQVAALCGVHCLNTLLQGPYFSEIELAQIAQGLDELERALVGEGALGEGSGNVAMDGMFSIQVLSRALESWGLQVVSLESEEARLFKVAPTTADAFICNLHEHWFTLRRVAPGEWWNFNSLFPAPQPLSTFYLQAFLDSLRGEGWTIFVVSGPLPVAQPRSLPNATGRYWSPNEARAATDEAERARKRGRLANALEEALERAAQQGGNLQLRTRGGGRGGGGGPGGAATSAEVEDEDADLAAAIAASLKSHQPSQQMHQQHQPAEGGIGGAQDWPGPSGGGGGGGGGAGGSQGPYEGMYGDEYEDDPELAMAIAASLAEVGQGGGEQPPPGSGQQLAPTPQQEAEADADPQDLEPEPEAEGEGVIEVAFRLPTGTRCSRRFTLTTPSSALFTYVGPLLSLPTARIALSTAFPKKELECSRTATLGDLGLTNRIMLVAEPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S651</accession>
    <name>A0ABQ5S651_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008944</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05282">
        <property type="entry name" value="ETR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF2">
        <property type="entry name" value="ZN2+-BINDING DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="17"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="341" mass="35696" checksum="BDC99551413D3F1F" modified="2025-10-08" version="1">MASLPEAQTAIQISAFGIDNIAVSQVPLPSPGAGEVLVRITARPVNPSDVFSVLGVYPGFMPKDFPAVLGLEGVGEVAALGPGTTGRISVGQRVVATNWRSASEGRGTWQQYLTASEEHLVPVPDDLPDEAACQALINPVPVIGMLQELATPKDDYIIITAAGSALGRMAIRYAKHLGVRVVATCRRAEQVDELKAAGADEAVVLGADGDALASRVLELTGGRGAWGALDSIAGSSPAKIAPAVRVGGTIIVYGAMGSPNIDWNVGQCLFRLVALKGFWLVPWLDAKPVEEQRGVLASVLEHMRSGLLPPMKVDTRPLEEAAAALRDQAIEGRPAKIVLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6B4</accession>
    <name>A0ABQ5S6B4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_008823</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF298">
        <property type="entry name" value="MIXED LINEAGE KINASE DOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="578"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="782"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="657"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1081"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1198"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1364"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1487"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1573"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="692"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1105"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1115"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1202"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1255"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1316"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1382"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1587"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="809"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1787" mass="188137" checksum="18EE640E35AD7FA7" modified="2025-10-08" version="1">MAAQPDSDLVTVCTDVTEGGTLVRVVAPSRQIRMVDLMPVFEALDLNLVSYTAEKDPDAVGGTLQAPLGRRKHSFRGRFLVTDADGHNLSDDMLDELVAALQEALHVRLSRLGRCNALQEQSRRGSVASYATDASIDPDSPSFHTVGVGAAVGLHAQASAPSLGQLSHAHSNLQPGPNYHPGCSAQHAHPQPQSHLHSQHQHGTQHLNPDLQHLQQPQYSLPQYPDHTPSIGSMLLSEHSISSVGSLIRAPSGAASDPPTVPSPPSNTRFDNTTFTTLQQQSDPVFLDPASQIPLTSSTTAAGVGGGMLYDDRFIGGVPATPFTTGAVQPYPHPHHHHTRSQPPLQPQFQPQDIYELALVFPQRASSASSTPPHLSGTLPAIATPSPLLDAAGALAPPSALTAPIPGRTHVSFLEPGPCVIATVTPTSATAASLSSRLPSGGLLVVPASSPLPLASISSSAAMSTDVQTELGAVAPSSVRASGNATPASSSEEERDGSYTWWSTFTAQDTVARIMSHPARSISAEADLGHAKSLMTKHNISALLVNTGNASPGFITKRDFLKVQFSKNFKRTKVKDVMTQPVIFVDINLPIEQCSRVLESHGVRRVLVHDPSRVSVEDPLSAFVGVVSDADLFKCMGAPAMSPVMGSMANSNEQAQPYFGMLPGNDDCLPPLPSPPSLRQPVVESPEPARPQPQVQIQQQTQAPLPVFVASSASASVPNSAGPSHAASIAAGLPLAADAASAAALALASTASAVVMRLNTEASLADRYRCAAALWELDFNELEVTRRIGEGSFGEVLLANFRGTKVAVKRLRGFDVGPDNPDTGAPGEPGPSPTQMPIFRQFFESLLQREIEILATIRHPNVVNFIGACHTPPNVCLVTEYCARGSLDHLLHKSSIPLDLTKKVEFSMDIARGMSCLHSQKPPIIHRDLKTANLLVSARFEVKVADFGLSRIKDHAQLINSRAGLEGTVEYAAPEVLRGEPYTEKCDIWSYAVLLWEIIHRQRPYADADVPIYILMMSLGNGTLRLPPVREELATCGLVRLVERCMAWHPADRPSFREVLHALEAEYKILRGKAAAVPRSDSASSMVVLPGGQARGPGADGAAQHGTQHLQNPQGAAAGPGSSAMAARPGTQPLTPPQQQRRAIGTEAVPQKKNSKLGLGAGPYTPGPEVSGGRCGAEPSLVHVHGQAQVLGQGLSDVGSYGTPNPHTQLLSQQPQGGRARVKLAVPAQATAAHHGSVTAGGTGGNNHDNADPVSPADSSSRIMSSRNASTSTAGRGAGAPGKPSSAGRTSGRSLRSAARSKSMPLPEFEWFGGEEGSDEYMDTTDSVADDEADAGASAGTSTSGATSGAIALAAQVQAHHPLSLLSSDRGGVRSTPYPESLQHETSVDDKQRDVIDSTADGAVSAFADDPTAQQLPHNLVIRVMRQDEAGAAPTMVTTSRGVSSHQVAEQQRHERAEMPAQPEWAGRVEVVAPVVAAVRQSPFHLASSSPFADAADTPWSPRGSEDAGSSPSCFDSRPVSEYAATSLVQCQQVQQQHPASAPCNPPVASVNWAPPRQTALISPFAQLANEASMSALREEEEEEEDQRCTEGSDRRDEAEERCWRLTVSGGGGAAATHTANSTDVITMTEKDQQTTVEACWGVLGTRSKPPSGNIYPPSPAPIGRLSPPTANTQGLPTLASPHIQHGDTVGAVDDGAASRRRGEDRNEGEVIVVVEDGGVEDRSEREQQLVAALSSASAGLEALSLALPAVLAFSGTGLAGAQSGSDQFDDEEDEDATLHTTAWVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6H4</accession>
    <name>A0ABQ5S6H4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">magnesium chelatase</fullName>
            <ecNumber evidence="2">6.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_009077</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>protoporphyrin IX + Mg(2+) + ATP + H2O = Mg-protoporphyrin IX + ADP + phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60492"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.6.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunit H family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.6.1.1" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10150">
        <property type="entry name" value="CobN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011771">
        <property type="entry name" value="BchH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022571">
        <property type="entry name" value="Mg_chelatase_H_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02025">
        <property type="entry name" value="BchH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009140">
        <property type="entry name" value="PRK12493.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLH, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119:SF1">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLH, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11965">
        <property type="entry name" value="DUF3479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Magnesium chelatase subunit H N-terminal" evidence="12">
        <location>
            <begin position="95"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="CobN/magnesium chelatase" evidence="11">
        <location>
            <begin position="260"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI65522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1280" mass="141069" checksum="C8C7FF61EC128F2D" modified="2025-10-08" version="1" fragment="single">MHHPSLLGRRAAHPASGLTSKPVVPNPRVASTRQVVCNVAIGPKPPMITFTGGNKGGNKPVSLDLRDEGTGLFTSTSPELRRVIPENVNGRVKVKVVYVVLEAQYQSAITAAVKNINAKNSKVCFEVVGYLLEELRDSKNFEALKQDVATANIFIGSLIFIEELAEKIVEAVKPMRDQLDACLIFPSMPAVMKLNKLGTFSMSQLGQSKSVFSEFIKSARKNNDNFEEGLLKLVRTLPKVLKFLPSDKAQDARNFVNSLQYWLGGNSDNLENLLLNVCSAYVPALKGVDFSVAEPQQYPDVGIWHPLAPMMYEDLKEYLNWYDTRKDMTFAKDAPIIGLVLQRSHLVTGDEGHYSGVVAELESRGSKVIPVFAGGLDFSAPVKKFFYDPLGTGRPFVDTVVSLTGFALVGGPARQDAPKAIEALKALNVPYLVSLPLVFQTTEEWLDSELGVHPVQVALQVALPELDGAIEPIVFAGRDSNTGKSHSLPDRIASLCSRAVNWARLRKKRNAEKRLAITVFSFPPDKGNVGTAAYLNVFGSIYRVLKNLQREGYDVGLLPPSDEDLIQSVLTQKEARFNSTDLHIAYKMKVDEYQQLCEYSEALEENWGKPPGTLNSNGQELLIYGRQFGNVFIGVQPTFGYEGDPMRLLFSRSASPHHGFAAYYTYLEKIFKADAVLHFGTHGSLEFMPGKQVGMSGVCYPDSLIGTIPNVYYYAANNPSEATIAKRRSYANTISYLTPPAENAGLYKGLKELKELISSYQGMRESGRAEQICATIIETARQCNLDKDVKLPEADAKDLTMDDRDLVVGNVYRKLMEIESRLLPCGLHVVGCPPTAEEAVATLVNIAELDRPDNNPPIKGLPGLLARALGRDIEQIYSGNNKGLLADVDCLQRITEASRACVREFVKDRTGLNGRIGTNFVTQLLKFTGFYVDPWVRALQNGEFAKADRQELITLFNYLEFCLTQVVKDNELGALVEALNGQYVEPGPGGDPIRNPNVLPTGKNIHALDPQSIPTQAALKSAKLVVDRLLDRERDNNGGKYPETIALVLWGTDNIKTYGESLAQVMMMVGVKPVPDALGRVNKLEVIPLEELGRPRVDVVVNCSGVFRDLFVNQMLLLDRAIKLAAEQDEPDEMNFVRKHAKQQAAELGLQSLRDAATRVFSNSSGSYSSNVNLAVENSSWSDESQLQEMYLKRKSYAFNSDRPGAGGEMQRDVFEVAMKTVDVTFQNLDSSEISLTDVSHYFDSDPTKLVSSLRSDGRTPNAYIADTTTANAQVRTLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6R4</accession>
    <name>A0ABQ5S6R4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="247" mass="26444" checksum="E39DA7645CBF1920" modified="2025-10-08" version="1">MYLVHLNTKGVNDRMMKTKNDTAVVGTPIAAISAGNSTIVSGILSAKASDETAFSEQPEYESLLEPAEESGRSSSKVSDLQTTDVPPAPPTSIQQISSFQVPVAISLNSADVEVDLHSPPRAADARCLQHEDVMEVIAKEEAQSDLTEARTSKLTPEVPGRQVLITDDDEDSDDQVACFEDQEEDLVAAMIGLAVAPTPKAPIPLRGLPVPQGRHIRFDAKGKPLESPERTVLRGLPVATGKHTRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7B7</accession>
    <name>A0ABQ5S7B7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="48"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="360"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="524" mass="55957" checksum="342FA67FA5CD3DE6" modified="2025-10-08" version="1" fragment="single">REIAEFIMSCKLYFPLLVVAALAIGWVANAQYDDTEGQGIQTGIIPNFPYRSCNTSNGAYRLAPIWAPLGGNQYCFTIQVNQDAQSCSGPCCTSGLHKIEFNVSISCLVAGSSVTATLNGVPTRVGAVFDKPPYGLPGSAVLRITQLGLDPVTAQGAQLCITLKPNRARQGCTTLEQLCSSPGFPAGTCTAATFDVACDCCPVTQVVQAQPPPPPPVVPMYRYCELCVAAKLVPPANDVRPYRFDAATCATIQQNIADAMNAALNVSSISPVFAPFSPNSTNCFNDTVLTCGLFNGLEVSTLESLFIEVSGLLSSFIEVAAGGSVCNPRLEGYSVEITTDGNSCLDVSQSASCFLPQTPFPNCTCNITKGVLPFTVAATYSQVKNSSNFTLSDEYCFTIAALPPTVPIVKSTCYQENDTIAKLEWYADDNVRSYVKGFVLYPAVGPVKKVSPSWGAVGANTLKVSLNWTNEQANGGLVCVAIRQPYTMDNVCMDPPSNQCYVSIFNRDDNNNNDYCCPTFRSGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7X2</accession>
    <name>A0ABQ5S7X2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SMODS and SLOG-associating 2TM effector domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="19553" checksum="9E3390EE0F033EA7" modified="2025-10-08" version="1">MVRRDAYESNSPSGPGGAFNILRLVGHSKHLSSGFVGFAIKTISVSASASVIFGITSYMVFSKHLGPAMPFLGGAAFGFVGGLVHRYLTDTEEAQLMLTRYPKVMEHHINQVEPMELRDVSFDKWLEDLRNNMRKQGVLISALYSASETLTKLQEEEEERILEKHYRTDGGAGTAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S861</accession>
    <name>A0ABQ5S861_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009708</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004710">
        <property type="entry name" value="Bilac:Na_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361:SF28">
        <property type="entry name" value="P3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361">
        <property type="entry name" value="SODIUM-BILE ACID COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI66102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="434" mass="44724" checksum="C83C7635E61EC7C8" modified="2025-10-08" version="1">MRAASLPPQCGNIRSRVACSLSATHNPTGCPALVKAFYTAGQPRGTKRFTTAPAKLPLAHGTPATISKEKRPDAGAPGALARDVACASSASSGGAVSSNGGDDGGNAPVVLSSLEEQAASFCAGFTRLFPIWVILAAVSAFHAPHLYLWFDNTCVTYGLMFVMAGMGLTLTFKEIISVFTKQPQLLLLGMVLQYTILPIFGYFISRYLTSQPGLAPGLAVGVALVSCMPGGTASNIVAYIAKGDMPLSIMMTTASTLMAIATTPLLTSLLVGTLVPVDPRAMFLSVLQLVLAPVLAGTAINQFFPAAVARVKLYTPFLATVVVFFIVGSMIAANVAVVASSGAIIVAAVFGLHSTGFFLGYALSKALGLSDRIARTNSIEVGMQSSALAAVLAKVHFAADPVVVAPCVLSACTHATVGSLLAGYWAATVKTEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8H8</accession>
    <name>A0ABQ5S8H8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">assimilatory sulfite reductase (ferredoxin)</fullName>
            <ecNumber evidence="7">1.8.7.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">VaNZ11_009339</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">DNA-binding protein that binds to both double-stranded and single-stranded DNA without significant sequence specificity to reversibly repress the transcriptional activity of chloroplast nucleoids by promoting DNA compaction and possibly regulate DNA replication.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Essential protein with sulfite reductase activity required in assimilatory sulfate reduction pathway during both primary and secondary metabolism and thus involved in development and growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>hydrogen sulfide + 6 oxidized [2Fe-2S]-[ferredoxin] + 3 H2O = sulfite + 6 reduced [2Fe-2S]-[ferredoxin] + 7 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23132"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.8.7.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Interacts with ferredoxin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Plastid</location>
            <location evidence="5">Chloroplast stroma</location>
            <location evidence="5">Chloroplast nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.7.1" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.480.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein,Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011787">
        <property type="entry name" value="SiR_ferredoxin-dep"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010029">
        <property type="entry name" value="PRK13504.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02042">
        <property type="entry name" value="sir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493:SF47">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="Nitrite/Sulfite reductase N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0883">Thioether bond</keyword>
    <feature type="domain" description="Nitrite/Sulfite reductase ferredoxin-like" evidence="19">
        <location>
            <begin position="122"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrite/sulphite reductase 4Fe-4S" evidence="18">
        <location>
            <begin position="223"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrite/Sulfite reductase ferredoxin-like" evidence="19">
        <location>
            <begin position="418"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrite/sulphite reductase 4Fe-4S" evidence="18">
        <location>
            <begin position="495"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="638"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="642"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="GLI65734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="662" mass="73853" checksum="8BCA1AB3C41F579E" modified="2025-10-08" version="1">MAFRHAVRGQSTSLLGQPLSVQVTMQALAGKQLSAGLSRPSRGRLVITNVATPSKVPTSRLAKRSKVEIIKENSDYLRHPLMEELVNDKTYITEDSVQLMKFHGSYQQDHREKRSFGSGKAYMFMMRTRQPSGIVPNRLYLVMDELADKFGNGTLRLTTRQAYQLHGVLKHDLKTVFSTVIKNMGSTLGACGDVNRNVMGPAAPYLNKPEYVHAQKLANDFADLLAPQSGAYYDVWLDGEKFVSVYKEDPKVTADRAFNGYGTNFENSPEPIYGAQFLPRKFKIAVTVPGDNSVDLFTNDLGVIVISDDKGEVKGYNLVVGGGMGRTHRDEDTFPRLADPLGYVDKDDIFHAVKAVVAAQRDYGRRDNRKQARLKYLVSEWGIDRFRSVVEQYMGKKFREFVPMPTWEYKDYLGWGEQGNGKLFYGIYIQNGRIKGEQKKVLRAVIEKFELPVTLTPHQNIILRDVDPAHKDEITAMLRAGGVQELVDWDSLDRMSMACPALPLCGLAVTEAERTLPDINKRIRAMLTKVGLPEDQFLHVRMTGCPNGCARPYMAELGFVGDGPNSYQIWLGGNTNQTRLAELYADRVKVKDLESFLEPIFAAWLTNRRPNESFGDWTARSGFPAVKQLAATAAPTLVKNTSGNSNGNGAASAGSEKQLVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9E9</accession>
    <name>A0ABQ5S9E9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000034">
        <property type="protein sequence ID" value="GLI66552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="120" mass="12960" checksum="82158E4F3521BB76" modified="2025-10-08" version="1" fragment="single">TDLQGSSITAKFAIIPAASCPAMGATSPPSAVEVADAGAASIFINKAAQQPQELYQPLELTQPPKPHPKTPERRLPPEARNWGLQDCRSWAGEQHWSSWAEVQAFTKTLEPTLAEAIREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9J3</accession>
    <name>A0ABQ5S9J3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BEACH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009507</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052651">
        <property type="entry name" value="WDR81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44662">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44662:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BEACH" evidence="2">
        <location>
            <begin position="317"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1278" mass="134958" checksum="B976173FE85DF1FD" modified="2025-10-08" version="1" fragment="single">MTSEYGDDEVAEGLLRLAAQLQTFAASVLGLELDYVMPDIQGLTGWVAGARSPLRQPVILHFRAPWDGEDAGAIPAQSPTISLPEPSDPVSLERYLDEIPAELLTDSTPSSVPSHPPPYLRTMSAAHTSSRPDSQRLPSKPEHPNLAVLLNPVGDRDALIAGGLLVPAASETTCRSTAASAAAMSSSPVPLPAPEPPPASLGISDSLPPGVATVGVSWHPGCSLAEYLRSPPAAASCGLYGNGPGDQHLRFVLLQLVAAVCHLHARGYTLGGCISPETVFLQPGGWLQLLPPPLDQPPSLVPQSVQPATPLPRLPPCLQNLPPTLPALTRAWQHHALSTLDYLMLINIMAGRRLGDRTFHPFVPWVTDFTMPPQVMLAGSSSGGIGGSSGNGSRGGSAGGGGAGRGGWHDLTQSRYRQAKGDLQLDVTFESSDVPHHIPQEPLSELSFCIYMARATPRAVLQRAVRSHFEPREYPPSVEAMLARTPDECLPQLYTDAQIFRSIHPELPDLQLPEWASSPQEFIHWHRALLESDHVSRHLHHWLDLFFGYKLLGPAAVAAKNVYLTTQPQPRTVAAVAAPGVGAWSATADGPGAIAQPYAPLGMHPPLFLSPHPPRLPSPRPISAEVPGGGAAVVASSFCSHRLRPQYPRGAQAQNMRQSGSWPTSFGSVAGGAGIQGASGGGGGGSLRVCSDGGPAGVLAQLDSLELLGTVASREGGLMLHEDYFPLLMALPPSLLESPRRSQFLAAVSPQSISAGAGMAWGLRKQHRHGLQQRRLRLQLHAEDDDLDPQAEIVSGGAAGRDSILSAYATARSMTGRARRARTAEELPVTTARGTLQRGQLDPGGWRPASGREVPLVQNGTASKWGGDGFLVGRRGQAFGQGRVEGRCGFPELDTEDTGKGWFEPAARDSREAPWPPAGGLQGPTWAEEALLEMRRRDCWAIGKVAVQLYLGRCCYGPHHDAGYWLHLSDRLPAAAGRFVRLCFHPQVTSERLLSYPFFGPALHAAHELLRTTLYDSGAEAPAGTLRRLERAKSPGSAPGGDHAANVPLSTAPPVLSLLPPPQLLPCQAGALAAAASEGRLDEVSEHPTALELCLPHIMHVLWQAADACDAFIDGGGNSDSTSSNSSSSFKAVAMSVGEGAAAGTSTSQPTLSESAGSAALAIQVSGNVYDILLVLMRLLSPDKVTTNLLPYVCACLSCFMEQQPQDAVGSLSRQRRLALPQRLLSAPLQTQLLAGSDLDLYVQYVLPLLLAALLCSEETVPTAAGSTTAGVAAAAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9L2</accession>
    <name>A0ABQ5S9L2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03822">
        <property type="entry name" value="GT4_mannosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46656">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46656:SF3">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="884"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1426" mass="158723" checksum="DDC5A7F82BC60065" modified="2025-10-08" version="1">MRRRPLTFGLRRVLKLNCINRLQRWSWVLLALAFAVSSSTWFVAHKHSFTLWPAHRTCAKGICVVTLSGGLFSGFKGQVSASFARGSVHNGAAVNAWSVAGWVRLHKGIDEVYDVTALSFGPMSLKFAAPAVPYVFIETAWPQIQRQYDYKPSLRGHCGDLAIRVSRTTTFSSAVRLCAQMPGCTTVTRNSEDSSTFFCHGVYTLDNRDPTWSSAVLQHNPLPQNYSWAVGVKSWNHFALTYHSGLLHFYINGILHQTQRISAFLNFTQVDLGWSPRWFESAFPGDVRDVRVWRRQLQHNEVVMSASSELPPSDNVLHLAAERLSAAPPVAKSGWRRHALPNQPSDCAASVLVPVLDRTPIEQLQMVPYHLQQHAGESCQVNIFLVACASQNVDLTVIKGAQQSLRILQPEVQLRWDACVRYGLQQAMTQDAESPYFAIVAASLLPLQGWLSGLVSDLQQHTDVSAVFSKILWVNGTIKSFGSRFEQAYDDCLARQVVRPVEMYAGYPWNYGPTMKTVAVQTADCSAWLMRSSGVSKLLENGSFLLSLGSYLACSDMALRLQQAGRSVLVSPLSSFIDTATRAQQEDPAALQSFDQTWGPFLEAQYVSGLAHNATATWVMNCGGFASIEAFNLVQHLEGKLRFRAQMQRGWPFCEHPDVLQDMPRAFSERVYRLRRLSSYLPSDIVIHHKDYRQLAAWPWPPNNSSAYLIGRYSWDLDRIHRQWAEHIKSELDEVWVPSKWHTNYLEQQGVQRNKIFVVPNSVDAHFYDPDIWDPLTLPHQRRLSFLTPFKMDDRASWQAVLVAYLKAFRDNTDVSLYIHPGANPLPAYRRDLVYIAMNTFLQDSNDTYLQSISLFPLPSSGAPHVHVFEQELTQLGLLRISASVDVIIVPTRDEGWGTPYMEAMSLGKPVITTAWGPLGEYIQPDFGFLADFAMTPISTEDKGLRGAAWGEPTVEGLIDTLQRAYESKDLVAMGRNARSRVIEKYNNELVSSLVTKRLTEITRSELLSSRTAKALDMAKGQIPLDIVMCAMLPKPRVRLSRTTPALKLAVVSTAPPRRCGIATFNSALMEYLQPLLPQGSSVEVFPLVWDTEIPALGGTSPIQRRIRQKNYDDYVSTARAINGEGFHAAILQHEFGIWGGAEGSLVVCLARMLEVPVVSVIHSLSDNLQDMHQCILQHLAASSKAVVVMSESSRNKLGAFHGIPSDNVVVLPHGAPVISAVNPVAAKAMLNLTGRTVIMTNGLINPMKGIDVVLKTLPEIVKEFPSVMYLIVGEPHPDCGKPCADYFEELVAAASDHRIANHVRFVTKFLDNRLLLQYVQAADIYVLPYKDRITTNSGTLTMALAAGKAVVSTPFDHAASVLPGRGVLVDFDSPLSLQDGILELLRDDSLRTNYQRAAREFSLGHDWRSVAKGYASLLANITISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9V2</accession>
    <name>A0ABQ5S9V2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045907403" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="58"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="244" mass="25823" checksum="4917F6C4A2FA3574" modified="2025-10-08" version="1" fragment="single">MYHCLLTMGRLKMVVLMSALVAALAVAWASSTDAQEYTYADYTYDVAFGEQTGLIPAFPFRGCVNKIGAYRIGSDIKSLGGGKYCFKIKVNSNCTNACCSTDLYKIEFNVSDSCIVQPAGEVKATINGVPTRVGATYDRPVNGRNGSAILRLTQLGLNLTTAANAEVCLTLKTNRLGKGCGTLEQMCVPPPTAPIGVCSVAMFDKSMKCCPVSSNQYPIPNLNVPPSPPPPSPSTPPPPPSPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA39</accession>
    <name>A0ABQ5SA39_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_009901</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF781">
        <property type="entry name" value="MYOSIN-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6" id="KW-0518">Myosin</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="8">
        <location>
            <begin position="53"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="614"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="6">
        <location>
            <begin position="648"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1034"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1164"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1235"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="617"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1193"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="147"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1336" mass="147398" checksum="68E72727FBE6FB7E" modified="2025-10-08" version="1">MATGIGARVWVPKESEWVRAAVISVIGQKLCVRRDDKQEVWLDSSQAHLCNNDNVEDMTTLSFLHEPGILWNLQSRYNDDAIYTYTGNILIAVNPFKPVPYLYGSNIIEQYRSSPREQLPPHVYATACAAFRSMLRDGAGQAILVTGESGAGKTETAKLIMACLTHLGSHNGTTHGAARPAEPQGVSGVEQKILESNPLLEAFGNAKTLRNNNSSRFGKYVEIFFDPAAGGAITGAAVRTYLLERSRVVAVNNPERSFHIFYQLVYGASPADRAAWRLPKSASGFAYLARSSCFELPGQSNAEEYQHTRRAMAHIGLSEQQQSAVLGTVAAVLHLGNITFMDTEHEGAVVVDGVSWEALQAAAELLGVDPGPLAEVFTKRQIQTREGPIVTPLSAQAAADARDSMAKVVYARLFEWLVSVVNTAVDEAHNGTSGGGSTGGAKAQQHLSIGLLDIYGFESFDVNDLEQLCINLTNEKLQQHFNQHVFKWEQAEYEREGVDWSYISFRDNADVLDLLEGRMGLMDLLDELCRFPKATAEDLAHKYRSSNTVSANARFMRLNRPATAFGVEHYAGSVTYSTQNFLEKNRDYVVAEHQSLLGRSRQQLLQELFAPEATASGEGGSGGNCGSGPRGQSQFQFRSVSTQCRRQLGELMSALSQLQPHYVRCIKPNSTGVPGEFNAPYSLHQLRCGGVMEAVRIACAGYSYRRPYAAFLEHFWQLCPEPVHALRQRLGERLLPTLRRHRKQAQSMGSATQDLSSLDISELQDAAGAVLAAGLDLARLAEAEPQYHLGYTKVFLRTNAAAALERQRLAVTNAAAATIQAHVRKCQVQRWYHGVRRAVIALQMYCRGFIARREAQRRRNERAARAIQRAWHCYLERLQFQRLRRAVTVLQAMWRGVLVRRAIEDIRLERAVLAMQTAWRGVAVRREYLFTLRFWRAAVRIQTAWRAYVARQLYLQILPLHRAALAIQRVWRAHRRGAQLAERLRTALLQYWIYSRAAVVIQAAWRGKVARRRTRKLRLQQRRARILAAFNAAAAENAQPSRPSPDTSRRTHEVRTPTLIHRKCSFTCGVGVVVGSSSSGSSSSVKGYGGPLTAASNRSADLTPLTGGEVVRADDVASLPDRDTRVRARLLELQDFRVSPLVQYWQRQEAAAADNITVVAAAAPRRAQGSQRSLPGNFPEDSRQPISTHGSLERSPSLDISSSESSPLITDCSIAVDALEKAEITYGRGYRRSRGRHTLEGEDLGESGSSSRAGMCTTGEDGTDSGSRGGSACTVEETVELCSGGLSNEPSYGQLQCARQEDDGSGGVMGMEEDDEEENAEALVSAIRSMEVYIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAH7</accession>
    <name>A0ABQ5SAH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Imidazole glycerol phosphate synthase hisHF</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="9">Glutaminase</fullName>
                <ecNumber evidence="9">3.5.1.2</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">Cyclase</fullName>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_010750</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The glutaminase domain produces the ammonia necessary for the cyclase domain to produce IGP and AICAR from PRFAR. The ammonia is channeled to the active site of the cyclase domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Plastid</location>
            <location evidence="9">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="9"/>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014640">
        <property type="entry name" value="IGPS_HisHF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF2">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036936">
        <property type="entry name" value="IGPS_HisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 9 10" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="12">
        <location>
            <begin position="48"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI66779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="595" mass="62706" checksum="3A78133D57D70C1A" modified="2025-10-08" version="1">MHTLSCPLAAAVEVSTSAPRSVCRVVTRSRPSIVACHAINSSNREVTLLDYGAGNVRSVRNAIKKLGYTIRDVEKPADLVSAEKLIFPGVGSYGQAIRILSQKGLVGPLKDYIESGRPFLGICLGLQLLFEGSEESGGCEGLGIIPGAVTQFDTTLGLPVPHIGWNNISPTRGSVLLDGVGDRRVYFVHSYRATPSPANADWVLSTTQYGGEFVSTVNRGSVSAVQFHPEKSGPTGLDIIAGFLQPEEAVAARGRERAATPSPSSPAPAPAPNSPSSGPYGLAKRVIACLDVRSNDQGDLVVTKGDQYDVREKEGGRDVRNLGKPVDLAARYFEEGADEVAFLNITGFRDCVMEDAPMLEVLRRASERVFVPLTVGGGIKALTDSSGRSFSAAEVAAEYFRSGADKVSIGSDAVDAAQAYYARGGVPDGSTAIEQISAVYGKQAVVVSIDPRRVYVTEPAAVPHATVRTSKPGPNGEEFCWWQCTVKGGREGRDIGAVELARAVEALGAGEILLNCIDNDGVGKGFDLELVGAVSKAVTIPVIASSGAGSPQHFSEVFHSTGAAAALAAGVFHRREVGIEQVKKHLVEQNIPTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAT0</accession>
    <name>A0ABQ5SAT0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000030">
        <property type="protein sequence ID" value="GLI66306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF444">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="105" mass="12377" checksum="100612814DF56DCB" modified="2025-10-08" version="1" fragment="single">KTAFRTPFGHFEYTVLSFGLTNAPATFQAVMDRMFRPFIDRFIVCYLDDILVYSKTREEHLEHLRLVLDVMRREQLFAKRAKCFWAQPQVEYLGHIVSATGVKMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCA9</accession>
    <name>A0ABQ5SCA9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ANK_REP_REGION domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="40573" checksum="563F705C2D9AEA46" modified="2025-10-08" version="1" fragment="single">MDIGPPLVDNGHSAGLRAPLSAPYVENHNHGLHRYNTKLPRLHWCAYKGDIQGVLQCITGACNLEEAVSLRNQHGRMVCGITALFLAAQRGHAEIVKLLVTNGACPVKPCFIQGSAEVCTPAEVASLNLHFKLSRWLKKAARARRKEDEVLALDDEDRMSQVSSRLARGLQRARSTYSMSAAPAAAAGGGMSISGVLARMSRGSRNGGAMSVDGGPSLSQLGYGREGPPSVGDAVEADPQRLAALNFMQNVDLAAFDPEGGDQPVVVNRVHGQGAFKKLVRRIISWGGKDIRTDGPGGQGAQTSVRASASTRVRGLNNGVSGGGLSYRGGLGVDYEDPTRPDHTKVLQRFLKDLDLAHWRPEHDGPLERTTIGGNSTRPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCK2</accession>
    <name>A0ABQ5SCK2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF58">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="43"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="235" mass="25946" checksum="A38B63B42BF60EC9" modified="2025-10-08" version="1">MRRGVAVPLSTRAQIRPAVVRSQVTVKCCYIAASPNLSLSAKCPYRTLGVHHNANEEEIKGAFRKKAKMMHPDVSKDPVAERAFMECQLAYKLLLNSEARAKYDKTLLPCKSNALAYLNDRSIARHGISPTRFSSRVAYPGRREVRSNLLSLVIQIRACVSCLEREYLGKDAIRRGLGAAMAHAAPSHLSMSGVQSSAVVVDSECGEHEGCEPWIYYAAQTVEEEKKAAAASRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD24</accession>
    <name>A0ABQ5SD24_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein 14 C-terminal Fig4-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_011781</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAC14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026825">
        <property type="entry name" value="Vac14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021841">
        <property type="entry name" value="VAC14_Fig4p-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023:SF0">
        <property type="entry name" value="PROTEIN VAC14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023">
        <property type="entry name" value="TAX1 BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12755">
        <property type="entry name" value="Vac14_Fab1_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11916">
        <property type="entry name" value="Vac14_Fig4_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="5">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein 14 C-terminal Fig4-binding" evidence="7">
        <location>
            <begin position="464"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="788"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="874"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="885" mass="94493" checksum="13EF58DBAEFAB198" modified="2025-10-08" version="1">MVASDELLPAAVLRQIGDKLYEKRKFAALDVEQLIKRLAAQNDQHRIRLIIDKLISEYSFSSQANHRKGALLCLAAAAVGLGEPTEVHLRQIVPPVLASFTDQDARVRYYACEALYNIAKVARSTFILFFNEVFDAMFRLCADSEANVQNAVQFLDALIKDIAADCTNFEVAAFIPKLRDYLRVTNPHKRQFLLSWVEALDSMPNVKMLPHLPALMDGLLSMLAEPVREVRATVAKCLKDFLAEVRATSEADLSVEFFSRLTATVVDRAGSPDELTRLTSIHWLRAFVELAPDRVLPHVPAILGVVLYNISSHNVDIQRECADANAALLRLDVGVYGAAARGAGAEGASGTGVTPVFGARAAVGNLDIPAILGTVTLEMRSEMEATRLEALRWLHFLLARTQELVLEQVGRLLPPLLDSLSAPSDAVVTSALGVLAALADCPGQFNPVLVAFLDRFRGEAGFALLQRSGSSLVRRLCSHLGAAAVLPELGAILQRDTDMSFASTMVSVLNLILLTGPELAELRDQLRRAAVDPAGAKLFTVLYPSWCYSAGALLSLCFVAQAYDHAVEIVHAFADLPFGAELLVQIDRLVALLETPCFTFLRLQLLEPRKHPSLLRALYGLLMLLPQCNAFRMLNARLQAVPTLELLQLEDGLGPTFGSHMGGVARAGQLHPAEREGDVAATAAVTKSDWAGFPELLRVFRAAQLRQYEAYTEQRRPQPLMAIGTPGPGVTFAANVTDRPTTLSTADKGGVPYVVSPTAAAGLAADATGLPARVGTSASASISVAQLGAGGPGATSGMSARPAGGPLGSSGDAQVAAAAEAEEDNAADAAVRGSGGPLGDDLSFSDNPFLTPPAAADIRQMQLQQPQPAAPNRGQLQHISSVGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE52</accession>
    <name>A0ABQ5SE52_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mRNA guanylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_012551</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:67012"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.7.7.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67013"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.50" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07895">
        <property type="entry name" value="Adenylation_mRNA_capping"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14502">
        <property type="entry name" value="RNA_5'-triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001339">
        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013846">
        <property type="entry name" value="mRNA_cap_enzyme_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF17">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03919">
        <property type="entry name" value="mRNA_cap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01331">
        <property type="entry name" value="mRNA_cap_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="12">
        <location>
            <begin position="126"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="695"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="767"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="716"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI68207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="811" mass="92984" checksum="DCE487E3496155D2" modified="2025-10-08" version="1">MYKSDIGLHEDVKTVPSRGYIPQLPDGWVKCPKFGDPRFFDGLNIIPCKVPLSTKFNSLVGASGRWTLADAIRQFRSYGMEVGTVIDLTKSRNYYSFQQEMEDLRFPDIHYVKIPCRGRGQSPLPSEVNEAVWHIFCHHEQEDTKHKYILLHCTHGFNRTGYIVVAALMRLRLDRGMSMKRAIARFAMARPPGIYKDQYINDLFKYYHALRDSNTITPPVPAWKGNNDVEDAADAVNEDEAGAGEEGAAGELPAVVTSHEDIWAIGERVSDIEADWVRYQVCALLGTVGANSGKLRFPGMQPVSLSIDRLKDLAAFRYHVTWKADGTRYMIFIAPHGTYLMDRSFNVVRCQMRFMNGNRSPLAPGQKHPYPVGKPLTLTLLDGEMVLDVDPTGAQPPRLRYLIYDLCMINNEPLIEKPWKDRYRAIDSHIVLPRNAERAYIDKWRGRTFDYARAEYMTPPLGYMYTEERFSVRRKEFWPTWQIDKVFQRFTNTHSIGHESDGVILQGVGDPYIAGTCERLYKWKFAHMNSVDFLLRCETRLGRNGMRELDLNKPEPLKLFLVDQNNRGRDIKYVELLKVDPDGHYQVEFPPDIDPVIYDGKLVECTFDCSRGMWMFMRERKDKDTPNASRVYLRIKESIVNHVDQQLLVGTLKDALLNQPAYVVDKAILNPEQQEKLRREVEAWHREMEEQRRRMAATCRPSCTGPHDEFDGEEPFSPPPASPPPPGYFDEDYNEDFAGAYMESGSVDGELNGYKVSDVDMDAQAATLKRPQDPGGDGQDEGRPMRRVTRWPFDVDAPCPDCPVYEGYGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEN1</accession>
    <name>A0ABQ5SEN1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_012235</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14499">
        <property type="entry name" value="CDC14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17657">
        <property type="entry name" value="CDC14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044506">
        <property type="entry name" value="CDC14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029260">
        <property type="entry name" value="DSPn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14671">
        <property type="entry name" value="DSPn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="184"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="267"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="539"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="585"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="636"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="769"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="590"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="662"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="699"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="715"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="769"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="811" mass="84249" checksum="26C8D28EE76C218D" modified="2025-10-08" version="1">MGAEDEFAQAIEIISGLYYFLTVKRADSPRYSPIAQSNICYCIDDELLYEPFFADFGPLNLGRTYRFCQKTKQLLQDAERNGKRVYLYTGPHAHQKANGAVLVGIYQVIYLNRTADVAYKPLSVYKPFVPFRDASCGVSTFHLTVYDVIRGIQRARDVGFIDWNSGSSTWSLEEYEYYEQVENGDLNWIVPGKFVAFSGPAARSNEVAGYRLHTPEDYWDYFRRKGVTTVVRLNKKVYDRKRFLDGGFKHHEMYFPDGSCPTEPIIQRFLDTVEAEPGAIAVHCKAGLGRTGLLICLYIMKHYRFTAEEVIGYIRVCRPGSVIGPQQNFLREMEGRIWHEGELYRAARGLPKAGPAVNDLVIAGRAAGAPDLVASPTPLAVSDAGTGVASANGRNGYHGSSSSAYSQMRSSNSGAVGRPVGVGSSAAVAAAPPAASDMSLLQYSKLQQFGPASGQYSGNGSYLSPEYSGSASSPAVLGVGASAAIGVGAAGPYGAVSSFRSRYNGGSVGYGNGAGVGRSMTPLRYSQQANVGYHLGVQNHHSNHTGSAGPFGVHHSSSHPGPLQAAAAGSRGSLGISSVTRSIAVPPAGPATGTAGAGSRSPSTGRLSVAAGLAAPPTSSAAAALRSTLHTETLAAADRPKLVPERNTQSADIGSGFGLGGTSLTPSGAGGSASPWRIAVPSGGSAGSSGPGTPNSVGSAGGGTGTAGASGSSVLNRTPSVNGRTNGGAPGAPVVAASASGGVARILAPNGQPRKVPMALLNQYVQQQQQPQGSASQQAVAGWGPAAGVPKPPAAAAPAQMRTSYPYGMRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEP0</accession>
    <name>A0ABQ5SEP0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012713</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="305"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="74"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="631" mass="69086" checksum="6FE3262F0CCF5851" modified="2025-10-08" version="1">MRRLLLARLHPEEVGSLHNTGHQSDGDSAVLNFFVYVLVLPGTLATCASWSRLWRWRSRRWHWDWRCRCFKSSEDAQDAQNTRNSFPQPSTRSSNGAATSASQGSTSQNDTRDATQLSTTTTDGPMHSAKPGQPANQVSLPTRMVGDIGHRTDTPKAAPGPSYESDDDEGGRLGTAEGRLNDITRSVKSHANAAGLGSQDEAARRQAEDAANRLAAERSLLPHDDAKGTVVTASGEGFSGPPAIAPADINMPQLPTASPGMLAPAAAPEMQPAAGGEACGATQPMAACDIDLDIDLARGDLKVDYSPRGLLGQGGFAAVYKGRYKGQRVAVKMIKKTAGSSLTDLQEDMNALMHELHILRQVNHPNIVTLYGGCLKPPRIFLVEERMEGTLDFYVHQVRKHDALSVREVVYFALDIACGLSYLHPRIVHRDLKPANVLLSHEGRCKISDFGLARTKIKEYLSTKNLDAGTTAYIAPECFMSMDNPDMHDLRRSVTDRTDIYALGIIMWEMLAGKRPWQGLKPLLVAYQVAYRSLRPPLFELGPRCPPKLRQLIEECWCQESKQRPSSQQVVDRLLDILNELPPELDDLSYPQQQAPALPRQQLAALPAVVTVVQPLDGEAILAAAHLPSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF55</accession>
    <name>A0ABQ5SF55_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="320" mass="34951" checksum="A3EBAC386FCCE03D" modified="2025-10-08" version="1">MTTEQPVSNRLIFGLDETKFGIKLKSQKLLVGPFELGLSACYMLSEPEERAAQQNLISIERFKPSLSVKDVYLRGKFELEPATRRLHYSKKLRLPFLDTELLKLRLTAQLPAPETSGGVLDRALGLMVTFQPERLHNLSIVRKAPQYALQLSPRVGPPQQVCDSIGFPGSVYFKAQTTLHVSDSASLSGVRARLDLHSLNAVIRLYDPSAVKPCRIGRNLARVARHLTAEEVEELGPTVGEVAPTVQPVQPDPDVIDKLAATSREWAHAVLVNSCVATRNLQHKATQAVEHVRSYLSAIAAGGSGSGSAERSDGTNGSCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFA9</accession>
    <name>A0ABQ5SFA9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hemolysin III</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013083</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="26609" checksum="58B1F115116F2DB8" modified="2025-10-08" version="1">MTAEESSSPLLGAVGTFIEQASHPGMKFRPAGAPAGAWHHTPWYICTFYDPYERINVWSHGLPTLTFVVLGALAKAGVVPGGFALSLFCFCAAMTHGSSALTHVWPDDHVLEKIDHLCIPFLIIGVPATAVMALRPSAPYHIMIVMAVVATAAAFLRPLHRTLAFVASGAVLFGYYHWIVNWNMIAQVVLQVAGAAFFVRNGGHSRPVGLQDHHMLHYCVTAGCLLHVVYIMRAAQSLDKGPRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFK1</accession>
    <name>A0ABQ5SFK1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012462</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022127">
        <property type="entry name" value="STIMATE/YPL162C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735:SF1">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12400">
        <property type="entry name" value="STIMATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047046334" evidence="2">
        <location>
            <begin position="23"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="312" mass="33901" checksum="A98AC7999EDD2AED" modified="2025-10-08" version="1">MECRDCWWLVFLVLFYTYRAAASPAAGPVAPKNVPTSECRIIQGPTAILAQFVLLCIVITALLIKRHQERPRRAWNVWLLDVSKQACSAGAGHLCGMAIAVVAHKAATGVSECGWYLVVYLLDCTLGISLAILFHRVMHRVARWRHTQLMAARDREYEHPWTEALLESGNYGDPPNARRWVIQAAGWVTCVVTARLSVGATVMSMLRLVAEMARSLDRTFAGQPDAELFTVMVGIPMLLNVGQAWIQDQVLKWSARRRIKRGGDALSTSSDAAALPAGGAVISGGGGEVTTVQVLVPAGRNRGTGGDKSNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFQ4</accession>
    <name>A0ABQ5SFQ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012669</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="40213" checksum="D5472B61434A2D9C" modified="2025-10-08" version="1" fragment="single">VIRIVLVVLVITIIAIIIVAFCCCCLLLLLLVIVVIVIVVVTIVVVVIVIVILITVVVIIPGLYRSYGVFCQDIKCDSFQDFWARRWNLTTTYMMRVLVYEPMIEGRLIPGGPRCTKLSAATAAASHIVAGKSPGRPPRQGAEEGADAGCLASPDEAATATAAGPGTAAAAAAENMATVYEASPTSAPPEAIDNNGLRQRRRAAESSDEAMPLQDGSRVNGGRTSVAAAAAAAVEGAASNNGSETGTVRPVSKLRRFVGLQAVFLFSGLWHILIFWYNTHVFSWRWCAFFMVQAPILTFERLLRQVSRKMGVRVPHALQVFAANFLLIAVARPLFFGPTDATGFAARNLEVGQAPLRALMDLPRALAERFGSSPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFR2</accession>
    <name>A0ABQ5SFR2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-dependent oxidoreductase 2 FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012942</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050315">
        <property type="entry name" value="FAD-oxidoreductase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43400:SF1">
        <property type="entry name" value="FUMARATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="5">
        <location>
            <begin position="266"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="82"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="140"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="178"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="474" mass="48707" checksum="5AD56E808D0E9D26" modified="2025-10-08" version="1">MRRIPSAQHNRGIRIGAEQCMQRSQTRCSPTVRATFAPHRARPWLFVLLVSILGFGLLITLWIRLLPSTVLGATAQAQAQAQAEQSTRVQAHPSHSSSTSTNIGSGPRHKPQAGSLASDPIENAHASVAVGSSLVRQAQPDSWQLQQQKVDHHRKQQQQQQHYYLDGGRGGFRGGIGSSSSGSSSGNSSSNSAKIMPSSAVTKQHEEHQQSSAPPSAQVVVVGGGLAGLVAALQAAECLAEAEAEAEAATPGSSSSSTSCVPVQCGGSAAAIAPSYAVDVLLVEKMPKLGGNSAKASSGINALHPEGGDSEVLFRGDVATSGGGASRPELVETLVRHSPAALSFLAAHGASLTGVTRLGGHSVARTRTVRGGANVGWVLMSALMEAVRKQPRIQVLEGHSLRRIITTGELEGEGSSATDGDKSSHVVAGVELGPAGGPDGGAHPPFPLSQVMCGALVLATGGFAANKELIQVCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFR3</accession>
    <name>A0ABQ5SFR3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="16792" checksum="5762702BF4C3488F" modified="2025-10-08" version="1" fragment="single">VEEHAAKAHDVMAIRCRGTDTVLNYVTETYMEIMPVILPYNGRRPLHRNEVVRLLRSHGKEATRQTHAAGRYPGRSVVTEAAPEPPVTIFGDDECDLYGVMPGSTKPGSAGGGGSGVTDGQPVSPGSTAAAAYDSGSWPQPDPLVGGGDGGGNGFWSASGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFW9</accession>
    <name>A0ABQ5SFW9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052980">
        <property type="entry name" value="Crinkler_effector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33129:SF1">
        <property type="entry name" value="ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33129">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="430" mass="48831" checksum="1995178D4450CF51" modified="2025-10-08" version="1">MALADKAAACDRILELFEDDADMCGTILARLWPLGVAEFSDLRDAWANVLGLPAEKIFLKRSFQGGGLSSPRRPAPRQFQLFWQALLGLPPIVAGSFLTLPSNVYLLGLRCWGSSLLVRHCYRRIFDRMMELHSSNMKRQFLITSTPGIGKSFFAVVLMCWLVQEKGVNTILLDFDSMKYLFTSNGTDIKVEVGSEMDFFDEAFDPKTWWIVDMATAVRRPGSTVLLATLDKQRYNDFVKLSGATTLYMPIWTDDEIEECRSRLYPSLTDAKVRELMWKWGNIPRYLLDKAMDEATQNKLEDVLKGCKWQDVIDCILAPDIAPQTSHNLVHLEVVGDHYRRKVMKPASPYVEMERKAGKDHITQMQNLVHLSMDTPALAATAGIFFERYAHRRLQEGGSFNVWQLGSSETACISQHLVSNWSPAAIFRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG13</accession>
    <name>A0ABQ5SG13_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3172 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021511">
        <property type="entry name" value="DUF3172"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11371">
        <property type="entry name" value="DUF3172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="28603" checksum="AB0B3830E1DEA0EC" modified="2025-10-08" version="1">MLGAHRACNSHRVGSVPRVKLVVRAQQQRRPPSIPRSPFPPPPRPPSGYSNEQRPTYPYGEPNMQPGSSASPQFSLGDAAPATSSPRDEVGSGSSMGLGGYAQALVAAAFVIGLGIGTWFDSEVVLTPANVSSTEIIDRAVPNADICMANGYSASVLSMNVFVTYNPFNVYISQPQVKGGCVLRRANVGLLEREKLVTPHDVDLCKQRMNTFAYVGDLKSEPEVECVYHSEEAENQYLEVLGNRAKMSQLLMQQPVEPRAEKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG31</accession>
    <name>A0ABQ5SG31_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">7,8-dihydroneopterin aldolase</fullName>
            <ecNumber evidence="6">4.1.2.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_012663</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the DHNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00534">
        <property type="entry name" value="DHNA_DHNTPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006156">
        <property type="entry name" value="Dihydroneopterin_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006157">
        <property type="entry name" value="FolB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00525">
        <property type="entry name" value="folB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00526">
        <property type="entry name" value="folB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844">
        <property type="entry name" value="DIHYDRONEOPTERIN ALDOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844:SF1">
        <property type="entry name" value="DIHYDRONEOPTERIN ALDOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02152">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00905">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroneopterin aldolase/epimerase" evidence="7">
        <location>
            <begin position="63"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="199" mass="21649" checksum="E63E1CE685A507AD" modified="2025-10-08" version="1">MNMSKWFVNLAKCAVGRLPSGALLACAQTPDLVDNAFLWASAVGHIALNRYSTAPPTCTGDWIHINGMRFHGRHGVLPEETRLGQNFLVDIKLQVDATRAGASDALEDTVNYAAVYEDVKEIVEGPPCKLIEAVAQRAVNKVMLRHPEVRQVDFTIRKLAIPGLPSVVDSVGVQIRRFRQRPCSAPQLEPSAGGNVPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGH7</accession>
    <name>A0ABQ5SGH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F5/8 type C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013574</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037">
        <property type="entry name" value="FORMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037:SF24">
        <property type="entry name" value="POLYCOMB PROTEIN PCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045080148" description="F5/8 type C domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="312"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1192" mass="128522" checksum="10A6A4D27FF1873B" modified="2025-10-08" version="1">MATHVKHRTMTWSVLLVLISAVQAQVIVSGSLATNATVILSSNSDTTAILSIRDGNDSTMWQSGACFPTGYTARPQMNALYARCNVSGACSWSRGSSNSFYALDGNMSTSATIRSYGGQATLRISLRTPMNITRFGLRGVFPAPTNNASTAATVWLYAVEYSGALQEIMAVNSSANNKFLVATGTWQNITGVIINASSGFSLTEVAAQYGPCFESASVDLGAEQYIASIRVRYWPGPMGLASWLLVSRDGLKYTDLVSWMGLPPLNVSKVDPLLLLMPQAIKARYITVKHQVAEVDYAKVYVWGIDAWPPPPPSPPRPSPPPRPSPPPRPSPPPPPRPSPPPPPPPSPPPRPSPPPSPLPPQRQQPPPAPSPPPSPLPLPPPLPSSPPSPSPAPPPSPPPPSPLPPGPTPIQPKSPPPSISPDSIPPPPTQSWNNFAGTVRAYSYMGQVNVSSNPASTALPHIVDGDDNTQWQSDACLPTGYTARPSMNPLLFLCNGSSPTGAPSSYCTASNGSSNLGAATDTSVYTGAGIALDRARAPAADAAFFAAIVPGGPRVVTRVSCKGFSTSNVTVWLVVQSAGQSTQQRVYVSTFVPSVDNYNWVHITGSWPDVVGLRLQSNASFTLTEVAVQSGPCLEQAVVDMGSVREVGTLRFRYWSPDAEYSNMSVSADGVNWTDVRTFMDPTLLAAVEIYLPEIIYIRYMRISHKVKEDGDFRKVYVWGIDAYDKYGRWGPPPAPAPHPWSLRAMLGINGIWGWGFNKYSDTLRKDNKGPNMYTAVASWARSYHNMHWDVSWPSHNPKYNTMATTGTDGMWWLNWDREYAAWKAAGLKVDATFQFVSDVFPQSAWGANVTDTAYKLGYAFGFHFGPSRAPPHCSLDAVEVGNEPWIGYNATFYQAVIRGFAQGVKDADPTFRLLPCALQADNPYGEDKNNGNFVGARIPPEVAPLLDVVNLHAYSWYRPPDLNGTMIGVHPEHPGSTMNAINNMINWRNFNMPGKPLWVTEWGWDAHLPGETCTTTLCVTQHAQAVYGVRGLMIMARKGVQLADWFFYANADGGSGVFERSGLRGSNSTNFPALPVYYSLSSLMQLVGSCGFLGIVAERSDMYAYLLGPPNGTTSVATHLIAWRPVAAGDGPDEPVVEASLSLNRTPAQAWTISGAGSTPVTLGSVIQMAVGPSNWTLRVSAVPLLVQLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHF4</accession>
    <name>A0ABQ5SHF4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013782</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18534">
        <property type="entry name" value="DSP_plant_IBR5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044212">
        <property type="entry name" value="IBR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47244">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE IBR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47244:SF1">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE IBR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="46"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="105"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="281" mass="30935" checksum="F76EF0D206712104" modified="2025-10-08" version="1">MKAKRDREEPCFICNHYHDHENGEPCQVCGHVMTQAERRHLEHSVLPTPIIPGSLYLGSYDTASRSELLRAMGITHILNTWPSNPALFKNSFTYHTVSSTPVDFQECFDFIDSVLKAEQKVLVYCMTGTSRSPSVVISYLMKHRGWRLVESYKWVKDKRPVISIKDEDAKRLMDFEVQLRGDCSAPLGFTVLDITPTFAPQVQPSQATTQTPETAAMPSTSGPSFGAQGTQGQWSLAGASAPTGGFRFGGNVQQPSASASRNPFTLSRQIGDAGNDMETGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHM0</accession>
    <name>A0ABQ5SHM0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013963</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="546" mass="57974" checksum="37C011313730E26C" modified="2025-10-08" version="1">MIVTSLSHINGSSFRAPYSVCQVRTVPRPPCGVLTNNTGSGSRQSFLNLHKEVLQDSEWYGPEAPDHTVSPAAAAASPGAVATREDVRLIADFTKFLKKQKQDLSALFCNEAILSGDSRLVCDPRAGHHTELLSEFLTSGGMRVGEELPHWFVNGNLRGAIKCWMATRPAAAALTRADTALDNGHRLALHRDLVASVSEHVPLGPAASLLVDHMFAAMPEWSHGSMQHLAGWELAEGVQATAEDVAMLHCFGALVYASRLDVAVFLQQLCGADPFSWAMAQVSQGVISEPEPWLLERFFMSKHSVGEALPLWFARWGVREEIAKWMQQQQATKLVQELLEARGINTGAPAGLTVEAEATLTMMNVAAAGGRDGRAVAMDQLEASTAGLMASVLARHGASPSALPAGSISCSRDVRVYDKADPSGTPYAIDLLATCSGSTGTDQGGVLAVLVAVAVQLPYGGGAVGDVAVTLRQAKERLDWLALLLQREGGLAPQAAIAKVVLLLPQQEQQQGGQAAVATLPGSVLEYVKQGDVLLVQASDLPYKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI48</accession>
    <name>A0ABQ5SI48_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013639</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat ESC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051243">
        <property type="entry name" value="PcG_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10253">
        <property type="entry name" value="POLYCOMB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="146"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="446"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="568"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="514"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="590" mass="61116" checksum="06E1D01A28770479" modified="2025-10-08" version="1">MNLVQTNLLREVASEVKGHGGLLQFKSHALKNLELRTVLKESHCSPIVDLVFNALDPDHRNLFASIGKDQVTIYDDEHMGDFLGVVVQYINSPSAYHHGGDVTCCAWVQMSGITKHELGDACLAVSGPEGVIQVISVVEARVITLLKGHRGEVVELRGCAGVPGLLLSLGLDSSIRLWDVAAGICLAEMASDAITLETHPEGGCFFTGHNGGRICRWNLELDMMTRIAGEPACAKPLGALDVSPSAATVPDTPGPSGGLQGLEPRQGPTYQLALRSVPPTPQQLALPGNPLGEVVDCVRCLSGERLAAKSSDGKLVVWDLGRGVQVLSLRVPGTHPPAGAGQGAAAALRNGRCRLSVTRDGAFICVGNPAGDVYVYDTSSGARAAHYSAQKVRGPARAAALSEDGRHLLTVHGNGYILRYEYIRKQQLRKRAVADTDAPDVVQVGGAEAPSEHEARDGADGDVDGAAGSSREASPEAFTEDDTSAALGQGTVAAEPARAQVKRPRPTDGDASGSYGGGSGSVDDGGGDVGGAESTGRRIKLRVRLGPRPCGGAGLVDGAGLSAGVGVVASGPGKDRDTSTDAEWSGAVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI77</accession>
    <name>A0ABQ5SI77_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_014081</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF91">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="530"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="604"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="43"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI69464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="631" mass="70047" checksum="3D0352BF0320ECFE" modified="2025-10-08" version="1">MHAVVPPIDAAKPLRMADGKIEQLPSVDPQAGSVTLTLQSLSFIVKNVKKGPDGKRSDLALVDKVSAWFEPGLMSALMGPSGSGKTTLLDLLAGRKTMGRVEGELLFSGIKPSLEYRRRYCGYVEQFDTLLPILTVREMLLYTAELKRQLSEPLESKKAEVDKLLTQLCLAGCANVRIGSPAARGISGGQAKRTNIAIALVTDPRVIFLDEPTTGLDSYTANEVMLTVQKLARAGVTVVATVHSPTAFCFSLFDRLLMLVGGRTVYFGPQDQRAIDYFQSCKPFLESDPEGLLGPGHNSAEFMVDIITAGDRDGKGPAYADAYETSSLALENATTLQNHVAAFKNAQLPPNLAKELAVKRATVTPWWWGIYVYVKYRTSQNYKDPAFLMPRVMDKMMISLLVMTLYLGIGNNLHPDNIINISAVLFMWSATTGFSAAAYVPSLVLERPLFVRERNDGLFHATTYLTAKLLDEILINAVSALGIAAYTFYGIQLLNNFGVFYVSYIVGICVGISLAYFVASFAPSMDVANAIVPIYAVTLLFFAGFLMRLSQMPPWWKWYSYIDFARYSWGAVMINQFKNHQEPWIDNQTVLEYYDLVGISEWGYIGYTSLFFLFFYICTAIVLTLKKYQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIE5</accession>
    <name>A0ABQ5SIE5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="475"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="735" mass="76998" checksum="6D358330643E5908" modified="2025-10-08" version="1" fragment="single">ARLLKLPRLAHATLKRQPQASFLRQHSELQPPVPAPGISRRSPVTPMLDVMPQQEHQRSLLPSRAVSEGSSAILRTLMLFGPENQSAVGPDTNISRGQPKSTAAATSPGRCRCSESTDATLQVAIRSNGQLTTDQPSMTQIEATSANNGTSNGLRSPWCTTTATARYAPSPVQPQVRPQAVMAAMPTVMVASPSAAPSGGVGLPGSRDEGRTGLTVSGGRSGILLPQPWDCALQDVCSNNDDDGLGPRTATSWLPCTMSESAAGIGYGPVRPSVRPKTQISRGLYQQRAQLQMQMQLRESQLQNLRMRHLQQQQQQQLRYQMRLHHSPSAAAAAAAVVEAAALAKSPESALPISCSSLLPYQLGWASMAPSTPQVLPYGTYGTELRQQQQARPTALPPSASPGGLMLPPRAVSVGRECLTQQMSLLNITIPEEMDEGVATDLSAAPSAATSVEESLELQLLSPTNGLGASPMMPSSAGIASSTSSSSSRALRSTASQGFGEAFVALVRAGDVSVAPTSSSSSSAVLRCSSQMPSSSSSTTSVRSSGTLVPLSYGMTPYITAAAATTIEPSRTINPVVVYAGAVNVNANAVTPEGGHSKQQQELQLHPPYVTEGPFFVEPGNWKGETSAPIFTPAMHITVAAGTPCEGVSELGMHEEEVVVEVVAPNFDLQVAAMAETLAPVELTAEEADGPDVRSIRIALSLPPSADLQRHEAEKTSGCAFAVTGTDPTDGGTWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJC8</accession>
    <name>A0ABQ5SJC8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014856</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47215">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47215:SF1">
        <property type="entry name" value="F9L1.8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="2">
        <location>
            <begin position="88"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="404" mass="43027" checksum="6D4C499A77A9FC48" modified="2025-10-08" version="1">MSRSVLVRHYQSASISTHNHVLQFGHVAGSRLVHWPRSGAILSRRCKVNATTSRGIGRGGPQDDMDYHETKLGPNVIASVKDLRSGGVKWGTATVVRNDSQSLDNSVRVLHLAVADHVDMLYGRRVKGVPDATRWIESYTIPGQCVGLRLPADPQSASSAPSRLFSIASSPYASRRDSAYVDASLIEVVVERTAGSDEAALADLGPGAQLEVTQVIGRGFSPLLDSYNGLTHALEEGRNLVLIAMDTKGVAAIRSVLNWQPVLAHASSHSLTALYLTHSPGKAAFVPEWDSWREAGMRLRPLYTNPSAGTDDDAIVGGNGASSGSEGSALSPAVKLDHERCMELLQLALFAQEGGFEGVCGGSSSRCSFLLAGLPGDLASALAKQLSAKGVQYEHILFAQSDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJD2</accession>
    <name>A0ABQ5SJD2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4159 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10068">
        <property type="entry name" value="BONE MARROW PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10068:SF14">
        <property type="entry name" value="CELL WALL ADHESIN EAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="404"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="449"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="524"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="605"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="668"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="724"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="749"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="802"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="812"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="823"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1080" mass="112194" checksum="04322ADED90B5D45" modified="2025-10-08" version="1">MNFAGELNSLDAYKIVRLTMHPLSCQHLFKIAFLLSTYIGAAVASNALSQRIVASSPMVSFYSSSTSGLLDPFWDPLGPGNLENQLRSLQFNVLRFSGESAGTAIPSMARAFVIPPLTGDSPYSDAEDVEILKLFIRSGGLVIVHDSLRQPQATQDLVAEILGYEGSWQHCEPVTDNARHVFSVSGTSTSYARDFLDVPWPDSLENARVVQVHSWCKHEDPDAVSYPLYTLGGNSMKVVVQAFSKVGSPGAVLWLGYSWQDGPQEGWGSMIREVINAFATDSFHGPVNASAIMDNYPLRLDAIATYTAELADDTIEMVRRYLSASVGFGVVCNYGYGYSYGYGYGYGYGYGGYGYGYGGYAKDPVPCYMPPPRPAIAPPYYQPPTPKPSTPLLPATPPTPAAPSQPAAPSTPSTPAAPLQPATPSTPAAPLQPATPSTPAAPPQPTAPSTPSTPAAPLQPATPSTPAAPSTPSTPAAPLQPAAPSTPSTPAAPLQPATPSTPAAPLQPATPSTPAAPPQPAAPSTPSTPAAPLQPVTPSTPAAPSQPAAPSTPSTPAAPQQPATPSTPAAPLQPATPSTPAAPPQPTAPSTPSTPAAPPQPTAPSTPSTPAAPSTPSTPAAPLQPAAPSTPSTPAAPLQPATPSTPAAPLQPATPSTPAAPPQPAAPSTPSTPAAPLQPVTPSTPAAPSQPAAPSTPSTPAAPQQPATPSTPAAPLQPATPSTPAAPPQPTAPSTPSTPAAPPQPTAPSTPSTPAAPSTPSTPAAPLQPAAPSTPSTPAAPLQPATPSTPAAPLQPATPSTPAAPPQPAAPSTPSTPAAPLQPVIPQPPDLKPPSPQPPVETTKRFLWFRNMTAHGVQGVTRPVVWFDDPDFASQLNLHYNNSKLWLIDRMKEPACSKDPPCTSCPRAWYPYPGSDDRGFPNRTLVLYFREPVILKHVTILELASRGIEEVFLLGWPAVPVPQLGLNSTLVYGNVTRSDIFTELLRTRLSACSTRVVAFVKPPRSDDPQRVPGRGYNQTHLPPFFQERAVGGVGIRLRAPLDRTQVPAQIAQVLFTGRVLYPQNISEYEQYPKPHRGRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJJ3</accession>
    <name>A0ABQ5SJJ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7755 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014384</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056657">
        <property type="entry name" value="DUF7755"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36330">
        <property type="entry name" value="LIPASE/LIPOOXYGENASE, PLAT/LH2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36330:SF2">
        <property type="entry name" value="LIPASE_LIPOOXYGENASE, PLAT_LH2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24938">
        <property type="entry name" value="DUF7755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7755" evidence="3">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="495" mass="51521" checksum="936D58D467CD9947" modified="2025-10-08" version="1" fragment="single">LACPGHSHQTQVQRSCLVYVGRDRGRDGASRSTSHGPGTGSGAWLLPQSLVSERRVKDTTSLTDPEAPLTVYTIRFTTGVSRGAALSEPYAAVNVCLIGRDGRAALHRVSPVNDPLESRAHTVEMCQLIGPDVGADCSMALSDAVSNSGTDQLGNQQQQLLNRPLSASGGALFSGYGNGQGSSGMNLVGRPPPPPPPKRRFQEGSVDEVSVLFPELGPLAAVLVGVERGTWYLDEMDVASSRTQHMDRFVCRRQLGGIAGEGAALLTPVPVGAVVYGEGEAAMILSKEQATALWSLNMSDYSELKRRVAATTALLVASGASLAALMGGTVVALPYAAGGVVGLIYQWMLMREVDHVMEAANGQQKQQQALAGGVRTPEASAGRKAADSIHSSGYITSRVLGSMPARLGFVCASATLGMALLSREEAGLADGAGGYESAAAWQLALGVLGFLTYKAALVGVSMREAGSSPGASVLGSKIMHNSGGGRTELEKYKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJJ5</accession>
    <name>A0ABQ5SJJ5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014333</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="155"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="468"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="587"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="642"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="886"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="935"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1182"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="524"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="472"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="587"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="644"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="688"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="699"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="967"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="989"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1036"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1184"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1219"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1244"/>
            <end position="1278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1335" mass="136402" checksum="53D311EA4EDD6276" modified="2025-10-08" version="1">MVYVAPSKGSHGGKSGAPKNGNKGNRGRGKEQDALASKADLLAKLTRQAEEHTRILERERAALGLPTRASPTGQAPSHPYHPQQQNQPPAASNARNRRANYHVYNDQRNKKVFDPRQVPGLGGGNGGGNGGGGGRVPPIVASEASDAEQTFNQTFPDSPNFYPGRVFAIPGTIPPPPNQPQQNKPDDGTIVVAPWGGPPQVVAAGPPSLQGQLPWQIGQPCPPGGRDPQIMGPQPMGMGMPQSQRGVPTGMGAAPGLGESQPPQQVYGGPLIGSGIDSAEGVDPRVAARMEAEARKEAYRRELEEQIRAKKQAKEAEARRRRDEDAAWEARAAAAPQPWDPEARAARRGGGGEPLRDEYGRPVANLRQRGGGPGSPNGVGPGGLGPGLPPGMPPPQLGSGMAGPFSGPVGRLTVELPNGGAGAFPGPGAGGGIIPLPFSGDVGALGPHSHGQVPGPPLTPLRLGVLPPPGAPGSGAISGGGGIPFDGGPTPMLSPSHIPPVSPGNRRFMAAMNDLIAGPSDEQRRQKEMARQRLQMDLEEQIREKRERQARERAELERQRQKEEAEVKAFQDNMAAQRQVELNRNAPAAAPGAGGQLSPQQQHQRLQQHESSGGGIDAGRPPLPPRRHGKKGKIIDASWLDAPKAPPAALPPLSSPPAVPQISPGRGLLGRRPSTGMGDGASPKEELAGGGISPGPGFIGRAASHDGATGAAGTAGGRGNVTTLLQVLQEEQSRMREDFARQAAAMEKLATDANRALADRDEAWRELQRVRSLLAGGGAAATVPGDDFLASWGIGTVIVDTHLVPKTTNSIPDPLPKPVASEVLRRGRTDPVLLDGAMRSGLLSPVARGGVGGDGGGGRGDGFAGGFFGGGFGYGDLGPLQGPVLNRNANGARRSGIPMPPGMNPGGAAQHRLGPQRIQSVYGYGFDSPPKSRYNSFFGLEQGPPASPGNVRGGGGGIGLPSLGPRKQPPLKPPPSPGILRGDHLPSPVKQTRGPQQSQPGGARARSGRPLGGGGATERSMRGRAASAAPLQSQPKSPPELPPVAPLPAAVAARTVTGPSGHVSRRSLEGMQKVLQGGHEEKLLRGLGSDDGSGSTGTTVEYVAEDVPVASRSDVRGRRLTEASLPGNTEMMSPGAAVDYLAGNGSVAASTTASTAVAGKAATRSFFSDSIASYRSLLSQRTLAAAATAATPEPEVEAGGVQANRALLSSEAISQELRTSLSEGGSSATGTGAPTPQEQGPNDNGNVGHSSGASGAAMSVRMATAVSTADAADSQSSDMPVQRPVRAGRRPSAQQRLPSPADGNAPGADSGSSGRITGAAGDVIAASAGTAVDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK49</accession>
    <name>A0ABQ5SK49_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18873">
        <property type="entry name" value="NUDIX_NadM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43736">
        <property type="entry name" value="ADP-RIBOSE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43736:SF5">
        <property type="entry name" value="NUDIX HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="39"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="214" mass="23370" checksum="1327D7AA5C7E8BE2" modified="2025-10-08" version="1">MQSARLLGRVGSKFTGNSRRRETGVVRMAGSFTYQYPRPAVTVDTIIVSRPRDRVPPQLLLIKRKNPPYKDTWALPGGFVDEGESLDAAAGRELQEETSVDPATVSLTQVGAFGDPGRDPRGWTITVAYAALVPTTNLGVRAADDAKDARWFDVSMLPLLAFDHKLVVRTALRHLAKQPTAVAVAGLPAILEAAAHKLEGPWRTDFHRSMSTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKI1</accession>
    <name>A0ABQ5SKI1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PhoD-like phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043904">
        <property type="entry name" value="PhoD_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689">
        <property type="entry name" value="MEMBRANE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689:SF1">
        <property type="entry name" value="PHOD-LIKE PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19050">
        <property type="entry name" value="PhoD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoD-like phosphatase" evidence="1">
        <location>
            <begin position="161"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase" evidence="1">
        <location>
            <begin position="437"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="639" mass="71512" checksum="78122598A12201A8" modified="2025-10-08" version="1">MPENPLQRPLCESNESANPTDVVLDLCGPISGKGSVIPENTCCCTKLQAPHTVGPFLKFWGLERAPSAPGPANGAVAAEIVPEARAPRVWLGSLLVVTQAQVGDVAVELKEGSPGSVLEAEQLDSFDGWVALRFRLRIPLAEQDTKVHYAVTVSGQPLRLHQGDGGFCFHVAGTNSQCNAHWGFYSCSGFTPDVPPEHREDKWRGITPLWNDIRAVHNKAPLHLLVGGGDQLYCDDVWTLPSLAIFLKIPVKTEDDRTRKNVEPYDAGMRTQVDAYYFHHYCCHFSQPPIADVYALVPQVMSWDDHDIFDGWGSYAPYLQDSAVFKGVFASALRWYCVFQQHCKVEEKLPVAVYEVGRRTAIVACDMRHQRRPDEIVPLEHYMKISMLCTQLPKDIEHVVFVSTVPVVYPHVKGARTVLNLLKNGFLQCLLGTLGAKQLNQFGEPELLDDLDDHWSAPDHREERRFLIENLQMLSKYRGCRVTLLSGDVHVCGLGRLLSYPEPSDEMLLHDFRYMPQVISSAISNPPPPGGLIALLNFCSHGGRINRATHHKMTRELRKRTGLKVALENKRNWCEVQEEPFGNSGHALVFKLRVEELPVDAGTIKDYSVQVPPLQKLNGEYYDTRRGGLLARLKHNGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLM3</accession>
    <name>A0ABQ5SLM3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NFACT RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015153</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039730">
        <property type="entry name" value="Jlp2/Ccd25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13049">
        <property type="entry name" value="DUF814-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="218" mass="25594" checksum="DFCAEB9F68C5B1C2" modified="2025-10-08" version="1">MVFYFYPRGHKPNTEDFMIYMGKDKYENEDLIAHGLPCDLWFHVDDMSSAHVYLRLPDGCDVKDIPQDALEDCAQLVKQNSIQGCKTNNVTIVYTPWSNLKKTAGMDVGQVGFHDPSLVYKVKVDRRNNDIINRLEKTRQERYPDLAAEKEQYMSQVRQARRAEERAARASEKAAKEERKKQEDLKSYKHIMKDEFMVSNKDLHDKYKSVEELEEDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMF8</accession>
    <name>A0ABQ5SMF8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="232" mass="23883" checksum="CEC34646C3373352" modified="2025-10-08" version="1" fragment="single">PPPPSPLPSKPPSPRPPQPLPPPPPPQPPLPSPPSPRPPPPPPPLPPVVWATSASGGQPQGTTDGATKVIGAPQKAVLKLRSCDVRPSAALGWTPGGRKASSRFLAVSFDRNPPITGAQARSVGVYLIYTGYLRPLVTAVKLLVVLDAEVQANNAVTVPIFDITRGDISPVLVCPGPTHFNISASIVTKRSSVSKSAFSAAAIVGARVEFSEAPVAAVTDLPMVAAVGLYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMG4</accession>
    <name>A0ABQ5SMG4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="251" mass="23974" checksum="BCAEF1D3340BA002" modified="2025-10-08" version="1">MKLHSVPGLRATRPASAGRRSVVTCLSANGGASPYPSRPGGGAARFSFMSGDEVPSDAEGDVPTVALRPSGGAFRGVPTQDPGAAGAVGVLSFGRPGGGCTRFNLPQSSTVVIASGPTSAVDAPSRFSRPAGGAARMFPSGLGPSSGGPTSGDGGSGGGSSGGSGSGGSNGGGANGSPADEPLGFSTLAPWALLYLGVCGALYWAHEAVIKKKPVKAAAPTPAPGEAAPVKSCCKKAAAAAAAEKGGKGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMK3</accession>
    <name>A0ABQ5SMK3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF148">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="397" mass="41998" checksum="800270D3DBC22991" modified="2025-10-08" version="1">MRPLAQSPAKAGSDTDASTERAAGGNVHPPIASVSLAGDISMEGKYCIVTGSNCGIGLQVARGLMRRGAHVIMACRNMTACQQAASVLRGEGLSGSCACRVVDLENPSSIRAFVTQQAEELAINTNSVGNNDNGSGGGGGAAGLRGGQQPQQGRRYVDVLVNNAGVMGVPPAPDGSDRHLTTNHLGPYLLTRLLLPHMTHGSRIINVASRAHYTGSLSLVPDPNGRAETIRNDTHHWFWQYCRSKLCNVLFTAELQRRYGYGSPFALTVDKSSSVSGVSVGSSRTLEGIVGGIGAHAVSPGLVDTGIARYLLPGQLQWLLAPLRPFFRTPEQGAEVVLYAASSSELQGRNTLFLHDCREKQPSAAARDPRLAADLWRVSAALLRMPEEGGEPDKGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNQ6</accession>
    <name>A0ABQ5SNQ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glycerol kinase</fullName>
            <ecNumber evidence="3">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_016146</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07792">
        <property type="entry name" value="ASKHA_NBD_FGGY_GK1-3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042018">
        <property type="entry name" value="GK1-3_metazoan-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000756">
        <property type="entry name" value="PRK00047.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="12">
        <location>
            <begin position="7"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="13">
        <location>
            <begin position="296"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="529"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="590"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="539"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="603"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI71029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="612" mass="65219" checksum="F1A956227DA3397A" modified="2025-10-08" version="1">MEFTECIAALDQGTQSTRVFIFDRDCAPIASHQADLPQIYPQAGWCEHDPLEIWRTVLECLSRTWEDAQQRVVSAGRPPLRMMALGITNQRETTVVWSRTTGLPLHNAVVWLDNRTSELCDRLAATHGGRDAFRSVTGLPISPYFSATKYLWMYDNVPEVRAAVDSGDAAVGTIDSWLIWQLTGGPLGGVHITDVTNASRTLLMDLDSLDWHDPTLAAFRCPRAILPRIASNAEVYGLITPTAATAPIYGNASGSSGGFASPIHPLGPLEGVPIAGCLGDQMAAMLGQRCRPGEAKNTYGTGCFMLLNTGQRRIASTHGLLTTVAFKLGPDKRANYALEGSVAVAGLGISWLRDNLGLIGSAAESEVLAASVPNTGGVYFVPAFSGLLAPHWRADARGVLLGMTSFTTRAHVVRAMLEAICFQTREVLDAMRRDAELDEHHGGLAALRVDGGAAANDLLMQLQADLLQVPVVRPAFRETTSLGAALAAGLAVNLWTWEEVFAAAAQPPLRPAHPPHMLAPEGPLPDGPLSHFDTDNGIENGGGNNGRGGGGAAAVPAVAATAFQPAVTPAAASRRYDRWRKAVERALDLADLAPEPGEDEQEYVQQNAVAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SR03</accession>
    <name>A0ABQ5SR03_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_017190</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000125">
        <property type="protein sequence ID" value="GLI71824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050881">
        <property type="entry name" value="LL-DAP_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42832">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42832:SF2">
        <property type="entry name" value="ASPARTATE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="4">
        <location>
            <begin position="33"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="414" mass="44121" checksum="2B40C899F72BAAF8" modified="2025-10-08" version="1">MSERKFRSSRVPSPGVFLLMDEAKTRARVAGVTVIDLSIGASDLPPPPEALEALKISVDDSTTYSYCLKSGTMPLLEAATGWYCTRYNMQLDPATEALSLIGAQEGLAHALMAVADPGNGILMTNIAYPSYFGAVQVAGLVPLYMPLGPDFLPLLELVPAADLERARVMLLNYPNNPTSAVASREFFQAAVDLCKQYGILLIHDNPYVDQVFGKEPSPSPLCLPGGRDCVLELFSFAKSHHLGGFRLGFALGNCEAVASLEALKAPIDFNQYKGIMRMGVACLKLPHEVVRRDALVWQRRAEAMADALQLEGVEVQLPKAGMYLWLRLPPGVEDDVAFCQQLVAATGVALSPGQGFGPGGRGYVRVALVQPEGVLEAVAVRIAEAMRRKAAAPAAVAAASIGPDDLVEVGSAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLN1</accession>
    <name>A0ABQ5RLN1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="11714" checksum="1B0C45A930A61067" modified="2025-10-08" version="1">MSEGMRVFIRKLFAANFEARPRPQRTSLVQTVLVVAVTPVAASSAQPQDSIRVMWENIEQASARSSNTWCPGRTAIISFSDAARCYGCPAALQARLYASLRSSAIGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMF0</accession>
    <name>A0ABQ5RMF0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="40189" checksum="D97C7B477A6E67FD" modified="2025-10-08" version="1">MSIREGYDAVLDNTPTAEPCTSLFSLPNSVLTTIMEFIVRRANPGQVIKLALVCRALLNTVTSAEGLWEQLSISHGWSRRPTVRQRAAALTGAYTTGPEGGLSAADFDRHWFHYYTHRMTCRRNVRRFVRLYSAFLSRPSKMALMPGAEAAALSACEQRLSVELPWELWELYRFRNGQAPGTLVTFADDMRLLGLHELVLEHHPGLRELRRRLAMLVRLPGKRRAELLRPGGSAEMEAEEVAREQGTGADNSCGCEVAAAATEAAAAAGARSVCSEGLTYTVGYESKSQAPAAAAAALTLFPGDMILGCDDDYGEDCSDDRLLVVASNPSSTRRFLVALDGQVYLARGMLSLAFFASSVATMIQKLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMF5</accession>
    <name>A0ABQ5RMF5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">SRA1/Sec31 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_000351</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Endomembrane system</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SEC31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040251">
        <property type="entry name" value="SEC31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009917">
        <property type="entry name" value="SRA1/Sec31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923">
        <property type="entry name" value="SEC31-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923:SF11">
        <property type="entry name" value="SECRETORY 31, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07304">
        <property type="entry name" value="SRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="5 13" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="121"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="267"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="SRA1/Sec31" evidence="15">
        <location>
            <begin position="1097"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="894"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="918"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1089"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1089"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="1100"/>
            <end position="1109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI58616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1227" mass="130024" checksum="8C7E49BAD191A33E" modified="2025-10-08" version="1">MPHLKQLDRSATGSFCPHASYLAAGTVAGAIDISFSTSSVLEIFPLDFSTSSDYLSPCGSVAAPERFNRLAWGQRLSDQSFPQLGLLAGGLADGSVCLWDPSVLLDPSAKEQHRVSLLAKMQKHIGAVKGLEFNGFSPNLLASGAADSDLCIWDLAKPSTPSLYPALKGSPGAPGSAIGGEITYLAWNKKVQHILASCSTNGTTVVWDLKRQKPVISFRDTNSQRRASAIQWNPDIATQLIVASDDDRSPTLQMWDLRNSVSPLKEFVGHHKGVLAMAWSPHDSSLLLSSGKDNRTICWDVQSGDIVCELSSANWNFNVQWSPTIPGIFATSSFDGKLSVCNLHTCTGSKVTETVNADFTVTQSIVGDATPLKKAPAWMRRPVGATFGFGGRLVSFTNNRSQHTDSATGAVRLQDTGILSVNQVVTEQDLVSRSQGFEAAIQGGNLQTLRDYCCTKRAALSSAEKQSAEGKHEAETWAFLSVLFEGDDARRVLLQTLGFKDLPAREGSLTSAPTENGLDAATARVEHLNLQSSDGAHTGGTLAATSPEQLLAQDDPDFFEKFGNDDVQQPLLDAGSLAAAAPDLGAVQPLNTAYEAGNAAADGHGHAPGHVPGGELEAEIVKALTVGCYATAVDHCLAVHRYTDALLIANTAGRDLYQRTMHKYMQKCPHPYQAIIRANLEGDYSTLIRTRPVAQWRETLAMLVTYTDRDKFRGLADTLASRLAQAGMHHEASLCWVCGGHTDQAVSYWARNCTGASSMKVEVLQNVIEKAVVMGMAPGVSSTSSSPDGVNKASQSLSELVTAYASLLASNGRMATALDYLEHVPGEASTTVAVLKDRIYRSGVAELPPGVHPPPFPFVREEVVPVAQAAAQQATQTGQAYSVYGAPNTAPAYGAPNTAPAPQAASTVQYGQQQTANTYAPQTAAQPQASSYGQYSQYQQTQQQPSAYTQASVQQPAQQAAPYASGYQAPVYQQQRTAQMVAPTPAPFTPTAAVPPVVPQTQQPVPPSQIPAMPQPTYMATVVPSAFVPTGVQQPVPAAHTTATAAAQPHFFVPSASTAAPVTAMGSGGGGSYGGAPPVAPMPGMPASGMMPAATSQKPAAPPPPPGPPANISIATADTSTVSAELRPVVTSLTNLYNACMPLANNPAKKREMDDNTKKIGQLFWKLNSGEVSASVVPKLQQLCAAIDAGDWHTANHIQVQMTTTDWDECGFWLSAVKRMIKLRQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMY7</accession>
    <name>A0ABQ5RMY7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000718</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF117">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="978"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1009"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1040"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1085"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1112"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="276"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="347"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="616"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="681"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="796"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="347"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="634"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="689"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="703"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="798"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI58932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1138" mass="119229" checksum="42582D113DCFFE62" modified="2025-10-08" version="1">MVAPFQDKKYTDKLPWQQVLSLSSANFSQAVMLTTPFTVGVFMVRDFQAARYGGREDAVDEQVVGRLTGLLAGIFSFSSFLTAYAWGCASNYIGRKPVIVLGNAVSFVSMLWFGMSGSYANALAARAFGGFFNGILGAWKCMIGESTDVLLQGKFFGYMSLAWGLGCIAGPALGGAFSRPCSRLPYLTLCGDGQLLRARPYFLACAVGGLTILGALLLSVFMLEETLPKAMMDKSLMASLRRRRERRRLLHPAEEEEDANFESDLESESSLDLGSSAAGACVKSPQKGQSGSVARKQRRWGRWGRRQEPVAGVLAPGEGVDADVENGKQPALAAANRARTEERAKLLSAHTERSHDYSSCPHGRGHHGRQPRATFAGKPPCTTSARRQDPGLPSDTDVDSDANSETASACRGAVIRASSCGGGATDSEDCNPCRRRLHHRHCIAAANSRTPLMMRLQGLLRLASRTYIGINPFTGSSRRRSKASNAVVESTGAAAARTASPAETDIELLRCRKPNCDVNHSSETSPETGGWSKAEEILGGELEGEGVKRSCLGTCSVQDRNCHGCSGGHGKAQAADEEAAGGTTNAISAAAAPAAAAPIAAIAMPEESGHDMLQASAPPQPLASGVTSESPGITGTGGIGGRGGSCEEAETDMAVHISTCNDSVRNSYGGSAAGVALARSPARTRGLDAPPSEWASPAAEQHPGVHDERREGPMTGREPRQDAQPEDHLGLTLPASRESCRANRAGGTCDGVSKDDGKQSSGCSRESRDEARGAGGRMVECSLLSATSLPADAPYPHGPSAVAAPSPSSGSRMSNARGEYCERGSMTSKSGHSGAGDVSVLSALATLPEDSSLHGAMAFPAAEHGGGRSGALAARDGLGCVKQPDGRLLASEGDDTALPGEGSDMRQVDQLDVDVAVAVVEGEEPKRLLPWFRHPQVVLTVLGYGATALIFCAIDEVFPIFASAPRTSSGLGMREEQIAPPLMFFGAVLMPYSLYVYPPLQRRVGTLRLTRMGLAASVMTCILIPLVADLWTASAIFAQVFLYFAMVVKAFAQCNAFTGSIIAVNAAPSQEQLGAVNGVGQTLAALVRGVGPALGGLLWAVSLGLHSAGQQFLTFGLIAVVAAANFGLYGFVRLPNMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN65</accession>
    <name>A0ABQ5RN65_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="3 6">2.1.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000821</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PanB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="3 6"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001452">
        <property type="entry name" value="PRK00311.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881:SF0">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="507"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="507"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI58998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="542" mass="56117" checksum="107F3D17177CDB1C" modified="2025-10-08" version="1">MSAGPVSHGFQNLRMTFPTQPALSSTLWLGEGFHHQRILAAWLHKGDPNGAAVEPAPGSGSPATRPRSLSYISRGATHSYEFLSQLDSSSRPVQPPVSSPAASVLAHSRMRTGLLPRIAFGSAIAKHLTGVIGHGVLMSSSGRQNLKDLSALATAMPAAMLAAPQCWRQMSVQPEAAVYSGPSPRSPARVTLRIIRKKYESGEPLSMVTAYDYPSAVHVEAAGIDICLVGDSVAMVVHGHDTTLPIRLDEMVTHCQAVARGSRRAFLVGDLPFGCYETDVRDAVRAAVRMLKEGGMDAVKLEGGSPARVEAARAIVETGVAVMGHVGLTPQSVSVIGGFRPQAQVADEALRVLDQAQALQRAGCFALVLECIPGPIAAAITRTLSIPTIGIGAGGQCSGQVLVYHDLLGMMTHPHHAKVTPKFCKQYARVGDIIGAALQQYRQEVEDRTFPGLRYSPYVISKQEMELLAGALRSRGLDEAAEAAVEEARKVAEAKATAAAAAAAAAAGGTTPSGATASGAVAGSGASEAVGPAAGGAAGGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN86</accession>
    <name>A0ABQ5RN86_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038925">
        <property type="entry name" value="At3g17800-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008479">
        <property type="entry name" value="DUF760"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31808">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31808:SF4">
        <property type="entry name" value="LIGASE, PUTATIVE (DUF760)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05542">
        <property type="entry name" value="DUF760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="440" mass="48461" checksum="3DCEB1F8D0D1414F" modified="2025-10-08" version="1">MLPTMRYRLAGAGRRPQFAHTVHCRPTLRRKITVRAESSEFGLAPAQPKTPYGEMLQYYLRMEPHLFKTAVDTQLAKLRDEKLRRREKEEELSASSDGTELALYRRMEEVREGEVRATLEDLMYVSILEKFLLLGVDMLPRMDGLVDPPSTNLKALTEGIHSKEALELVREHLLSVMGSAATAYSNAYVKMSKFQMAQVYAASVMFGYFLRRVDQRFQLEKALGTLPLSKEDAVARLERLFAAAEDVERSDNPDFAEVTTVDLDSPTSSRSSQDADAPAVSAADRTAGASTSGRAPGSRRAGSPDGGRGSGSKSALRRYVESFDQATMVETARVVSVEGAALVERQTSALLGDIKKLTTQMQEAVGDNASSMQEAIERMAKAVELDMVETVTMAVATQRRSVLEAVAFGSFLRDVESWVQDEYALLTPIPPPRLPPAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNE5</accession>
    <name>A0ABQ5RNE5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="28417" checksum="DAC380CDF6C159D6" modified="2025-10-08" version="1" fragment="single">MDVLSVLRVVPPDVLQGGLWNVLPSASRVAFRQTCSSCDAWVRHNLTTRSSVIFNHLYLESLGSRGRSLAQHFPFLRSLTIRQASAGRKPLPYQVVCACLELLCQGSDTPNDSHALRTSFCYVRELVLRDWPSVGSDELALLAKACPCVEDFTVICRTRCFPHCFDIPADLLHALTVHFPHLRRLELHGIRLELALLLIQQPSQHQPSQHQPSQHQPTQQQPSQQQPSQQQPSQQQQTQQQPSQQQQTQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNM8</accession>
    <name>A0ABQ5RNM8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vitamin K epoxide reductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_000990</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VKOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12916">
        <property type="entry name" value="VKOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.130">
        <property type="entry name" value="VKOR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012932">
        <property type="entry name" value="VKOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044698">
        <property type="entry name" value="VKOR/LTO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038354">
        <property type="entry name" value="VKOR_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34573:SF1">
        <property type="entry name" value="VITAMIN K EPOXIDE REDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34573">
        <property type="entry name" value="VKC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07884">
        <property type="entry name" value="VKOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00756">
        <property type="entry name" value="VKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Vitamin K epoxide reductase" evidence="11">
        <location>
            <begin position="85"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI59163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="412" mass="42338" checksum="0D6A9AC6E12ACC46" modified="2025-10-08" version="1">MSAATIRSAGAVSCSGRGPSLALHGHVNVIALSRSRSEIGLSHGKRNMFPIRCIGPEQLPSAPQLADDPPALPKAEGKVASTPINTSNFNAMTALASVGVIETAYLTYVKLFNGVLICPTNGCESVLSSPYAQLFGIPLPFFGMVAYGTVGALAITYLRQAQDNAPAASLQTTLMGLTAGVAALATTSAVLISILQTRLGGTPCLWCYLSAGISTTLAVTLGTSLSGKQVKASGGVEAKESALGGVGGALATAAVLYVGLPSAAGSGAVYDDNFSLPYKAPEVATKSSNRAIDLAHRLNAVGARMYGAFWCSHCLEQKEDFGSAAMAEFPYVECFPNGWKRGEKVAPACEAANVRAFPTWMIGGRTIEGELQLDEVEKELLRAEAAAAAAAAASESVNTATAVAQMVAVSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNN1</accession>
    <name>A0ABQ5RNN1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_000709</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01908">
        <property type="entry name" value="YafJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF8">
        <property type="entry name" value="DI- AND TRIPEPTIDASE DUG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="9">
        <location>
            <begin position="2"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="503"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="843"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="883"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="605"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="676"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI58924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1840" mass="191633" checksum="BC690B1E3AF0943F" modified="2025-10-08" version="1">MCRLMAYVGQPQLLADIVLWPDRSIIKQSYDARERKIDPTLPFHLGHGNLNGDGFGIGWFSTEERREGDPSPCVFTSITPAWNNENLSRLACKIVSPLVFAHVRAAYPGMPVSEQNCHPFQFSRYMWMHNGVVAGFAQIRRALLETLSDCAYNAVASFHSDSAVSFALFLNHLPDMRVQLAPDVMIRAMQDAIATISAVQRRHGITGTSLLNYVVSDGSTLIATRFVSPESDSPASLYYAEGASFARTAATPVTPVATTVTTAGCTGQGAAGPGYAPCSASANSGSSITQESSYELGVSAQGPSVVFVASEPITASASDWVAVPKNTALVVTREKGGFLNILRCPLRSQPQPQLSPAPSSLPSTRWRTGALQPPVSAGGVPTAPGAVPACIDPQGDVCRCLEALSRGITAKSRAFLAHRPTGLVAGQGSMVLSPAPETPQELPFMTSIAGLAASVTRNSMGGQMLQLEALGATAAPAASRAGSQGLDATVPPSDADAHDFMRLVGHTGAVMTMTVCPYTNRLYTSSVDCSLRVWNLHDNSCLHTLIGQRKPVTAMRLRAQGQLLLASSGRKIRVWDAIGFRCLQLIKISDSCGTIRCLEVSEAPRKPAGATQGRGQEQEADGDTPYITGAGSRINDSGRSSLTGDDFASTGTIMAYGRSSSTNTAGLKVGGLDDPALPDIGAPGDHEGSAVTDSASRGGVSGVCSARASGAGDLSLLYVGCQDTTVKVFTLDEHELLTVAAAAQAAAAAAMVLEGSAASCGEVSQASHAVHSSTRTSRPAGMTTLHPSPFLPVRPVSGVRPRTSFTGMSGPPALLPPRIIATPEEQEHGISDLVPLMVTAAEDGAHVGPVNCLALCGRYLCSGGGDATVRVWDAATMKLVSVLRGHRGSVLCLLGLGSSTLLSGARDNTIRVWDLEMDMMCRLTLAGHKDDVTGLTSLTLQRRGAGGAVTTPPLGHTTSSASRCAAVAWASTMLASSSADGTVRLWSTSWTCLCILSVPASTSVPASLCACMSINMAMAGYSDGEVRLWHIEDVNRALLSTCYSETATVASAASEADGQHQASCACLAHGVAASEVLPSGGSSRTGGVQAALAHVRQHGASGAVAAAASLMGQGGSHGTGGIHEALSLLSPVVQGVVELGVGATAADMVKAEGRDSAMPTPSTEAAPETQFTQQQQEQIASGGAATEVVDFTASTASADGEVSGSVGQAFKTAADGNLNGATAGCCPTLSQTLPTASSTGAVVPIHIHGRRSPLSGTSMVNLAALVGGCAQAAIAATAAAAAGGGLATAAAAATLTTGQLPGGHLPLSLLGGHFCSTSDQQLEASLRDFVRIKTVSNSPKLRDDCHKGAKFLAKMLEGLGADVKVVQVIEDKNPVVIGRLGHNADKPTVTFYGHYDVQPAAEPDWATNPFELNSVNEYLYGRGVSDNKGPILAFIFAVRELMNNCGDATGAGCGGYSGGAGGRLGRLPVNVAFIFEGEEENGSRGFSQAIMQNLRWFEGTQLIIISNTLWVGENMPCLTYGMRGMLSLTVQITGPERDLHSGNDGGVFSEPMVDLIRVMATLVGPSGKVQVRGFYDNVEPGLIELAWTSLEESEEFSLEGYKVALGVPALTAPPNKRDLLVTRWCRPSLSVVDMRPGAPVAGAGQQSEPTGTCYRFGPTRFSVIPKAAQGKVSVRFVPDQDADTLVDCLRKHVERSFTELGSSNKIDLHVEARGNWWEVRQGSPWLAMAEAAIHKEWGVHPLYVREGGTMPVASYLERMLRAPAIMIPMGQSSDNCHLANERIRRTNLFKGKNVIRRLLEEIAAMDGVVAAGSAAANAPDGCDGVGVDMGAATVCAGREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNV1</accession>
    <name>A0ABQ5RNV1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001076</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="3">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="5">
        <location>
            <begin position="543"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="999" mass="106449" checksum="2F061089A1DF37BB" modified="2025-10-08" version="1">MLVQPHVVTLLLPILAFGVILGAGLWGATQLATDSSRIPNKQAGLGCAVGAHILLALAATGVTSALFISRRRHRDLLGALLPRQVLETLDNNQATSFGRKPADTPAYLLLNMMGDLLEGKAPRAQDVLFIRTALMSNMDVYQPFNLRATIKDANLDADVAQSLMNQLGGFSVDCATTVHLSGNANSPFRTLTRAGAGGAAETSEFVPVPVEASVSPSNSEQPCESLTSALALLLTPQPAIWQDVSTVQDDLASDLTTGPIDARELKPPVLPTVAAAAAALVLDRRTPNNCIQGQPPPTGNFLEKAASLIDRNRRRRHMGGGDVQGPPTAVVDLARDAVSRSSLFGVGSNILGRRGSTAGNANGNSNANGVRLNFRRASFLIPSATTDEHISATVNPTANSSVGGGPDDDSVTCTTLDGESCGGGVALALPTGEALVPRRLSAVWGMLSKRGTGNLTSSRKVSLDGNPNILCSSGGRASPAQSPASFCVRRASSTQSPLIHVASELLQQAHSRLGLGGAGGGGGVFRTTSVRAMVDVNSTTVNSTTNYPATAAAVIKRSMPPPLSVIDEVERLLAGADRWQFNTWALQEATQGHALSALGFYLIQKAGLLKAFHIKPVTLARVLRQIEAGYLDNPYHNAVHAADVLQTLHVVIHATQLHVHYLDRLGLFAAYYAAIVHDYGHPGMTNDFLVAISDPLAVRYNDRSPLENHHCAASFGLLQRPELDFLAPLSQSERSNFRKQVIEMVLATDMKQHFSILSHFNTVHRLAAYSQAAPAAPSPYKHGRRMLRRTVSHALSNNDTTPDPTSAVFGVYWNDPPPKPVDETERLLSLQMALKAADIGHLGEELEVHKRWLGVLEEEFFRQGDRERELGHSISPLFDRAKQGVSKSQVGFYDFVALPLVHALSSAFPGAHQLMNDFVRNYNHWRQVDGQLPVLIPSGKVGVTGRCSGRAVSSAGTGVSAGMPVAASIPRNSEQGGLSNLLLTVPDDALESRDNDAHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNY5</accession>
    <name>A0ABQ5RNY5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1824"/>
            <end position="1847"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1859"/>
            <end position="1879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="586"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="675"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1398"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1652"/>
            <end position="1683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2015"/>
            <end position="2064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2214"/>
            <end position="2437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="686"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1404"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2046"/>
            <end position="2062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2223"/>
            <end position="2254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2354"/>
            <end position="2371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2405"/>
            <end position="2418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2419"/>
            <end position="2432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2470" mass="257673" checksum="96C471DF9093C6FE" modified="2025-10-08" version="1">MRAAEQRLFTPFPRSCTHVVALQSLTLWLASWVYLKRVADQVFCSAEPDRYYRHLGYFRDAVIGYPCSEPGQHSFGVRGFAYFAIRPHLSLRWHALNAWNGVEELSETCIYFINLVFCLTTAIFPGSRMQRLLRRVLLVAPFFLHHLLTLLPLVALRPHIPSVLARLIPLRPFFVLYFALTGNHCLEVALAAAVALGSTIATTVLLLWAGVPLHFRPVPVLVHIVLASLAAVGWAALWDGLLPGVLLLRRLRLGRSTASGTSGLPRQQFLSTSTEGRLEAAAAGATCSSQAAPAGVPEGMALVVAAEMGQTGKHRHDGAVTSFSRRTAGTDRTKIENGGLRKWKPCAPRRRLGSLSPGMQFGVGMVASSTEESMSSFCSAGGKGGDVRDSSGCRCTCRTLGYRMVGIMHQLPLWPFSLLHKTSCHGGMVPAAAEAPRGLCPGDASALWRREERQMQVAGDMATPGLSPCCKGIRPVVWGPRQRFASQVRRQVLRIRIVGAEPEQLPSDWQERLFAHFNGHRNTSRYLSHLADVPTPQTHLACGVAVRRGSIFMTVSLVQLPCHHDGDDGPGGSSDRRMRRDVDQGVGVVGHRPDQPGSVDWAAGARSDGQKGGESDSASGTIAAAANTRQAPPPVVQQEQQTGPRCHVLVRTISAGADGCLKHADVPSAYGRSIDGRPAGADTHPASATTATAANAAAMSTANSSQSPSCGLAHSSPPMQHLVQRAPEIGGSGLHGASPSGCTSGRRRIAGVWRTERHGAFHSASSASTPGNSNNPLHVLGPVQSGTLCPGSPLATSFAGQTAGSLVLPAMPTPPPGLGLGPGGSQQPMRYTPMLSPTQQVSICSGLGYAPPACGANLASYSTEPSPGVATVVQLQHRGGVWPGPASSQTHGSVRTGGGTASASMQVAQLLDAMQLCSRDNTIREARHGCALSVLTAVSQLDGGIPLHLRQVLGTESWEQDDGVSSSWEVDGVYERPQPPLPLAPQQQQQARRWAQDLATAAANGLVMLSDAPSPQQLTAPGILNSGGPSPLLHQHLVLQQAQALAATAIVADGAGAITPPAHGTSGDARPVRGPAAPFGFPGTVPLVQPGGATWASVATVWPSHSTGLAVLADRATLPVRDVAVKPILICKAPLLHAAAAGDLASSIPSGLDTATMTVTPDSGPDVGSATAAVTAVPPPSELRLSLICAPATVSELSVMLRYCGPASIFGEQTYLPVQLQLDSASPAVAAAIRLQAAASDRRGGGDTQAARDVAEAATAPLPAHATVRTALGGLASPGVLYVELWHGPQLCACLPVLLLPESAAAWAHEVCSLWQQQAEEGEAAAVVQGRAGAAAMPAAAHQVVEDLGGWLTYAAHKRCVAERAAAIATATTATAASQPSLGLYRVCYHQAGPTAPGDGGVAGSSASTGTGATTSPPPLLDEWFSGWLVDTMLTEATTGAATLSPPAATTATPVQNQHLPLLPSRPTHTAMPAAAGYYPPYGGSYDAVGAAGGGGVSGACNRELFHDIMLQEGYNWLADCVEAGCCGLAAALMDSLLSAGCSAGEVLRTCRSGDGLPLIHAATLSGSPAMMDLVWGWGQATGMDLLGWEPLEQMPPVAAASAVAITGVAGVHVAAIKEPLHGPTTGGTSSMATTMDSTVVTRDSQVICHTGRAGADSTERAGVGGYDPASIPLQTRKDSTQNYGDPADYGGWVSWLETETLMHNTSTGRLHLLTSMLSLKVGQAASRLAAATQLMQQTMLPAISVTCSRRHRQGSGADRVNSSMEGLWRWWRAGARRGVAAALRLGDAGMMSLHSLVLRVNVLHVDGGAEERRFLSHFQQRHGWVFSAWCWSMIPVYALILWRYLLERRWGQLMWTPLWMWMYVVCACADVSGATAAYRSRTEALAAAMHLSHTLSKALHCAGLPSYPVPQGRGRLYWMDVLACATLSAICEPVRICVAWPLRLVNFIVSTVLYRVTGTAPTYLEASLLSASCHCFGLLMQHAVESHYRRSFTIMQQDMASGMTAGAVLIGASKSPDDSGLPDAPCSEATAATPTGYAPEQHQRQQPAQVSSPPGSPPSMSLFISAPDDIAMVGFSAPEPDASVTGQPEAVTAASTAISTAAAAVTATRTVQPAADAFGLSGEAVAAPASVAAVAQSDTGFGEVWELLERDRAHFPDRDLDRGRGRGAADSISGAGGYVIGARCLGSPLLEPREEVLLADGLWESSKVTGAWPFDHTPPSGALTTSTSASVHPSQSPVRPQGHLSTSPSLSSPREQWRRRQQQRYSQAHLQAQVPEAHGEGQALDLYDSVGPEANGRDDGAYLRTSAPLPRPPAQAGTGALHPGTTLPATATSDSRRFCTEQSSAEVGCESNGCGSNTAHRVVSPSTSRSLAYCRVNGSSGGAASGSRSDSGVATGRSSDNGGSSDTCSCAASSSRGSGRGSIASGGGRGSGRSLRQLVSRATGWVAGTFRRKSLRIAGPGGRKVNPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPG1</accession>
    <name>A0ABQ5RPG1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001033</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="5">
        <location>
            <begin position="502"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2177"/>
            <end position="2218"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2307"/>
            <end position="2348"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2348"/>
            <end position="2379"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2425"/>
            <end position="2466"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2632"/>
            <end position="2657"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2667"/>
            <end position="2701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="325"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="861"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="897"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1046"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1129"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1866"/>
            <end position="1892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2277"/>
            <end position="2298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="903"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1129"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1153"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1881"/>
            <end position="1892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="2287"/>
            <end position="2297"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2718" mass="274934" checksum="686C3E75D81B1A41" modified="2025-10-08" version="1" fragment="single">LHQFPTCPIPSYILKMPPRKAPAPAAAPAPTPAESAVSLEFLRAFLRRIPGGANGGATIARVVEELIRPSTSATLSSYASQPFLVPRNHVSAPTWVVIHPWDLPLADLVAVLEEFTMGLPPASTYFWLRALAVSQHLMGANAALQPLEEIEGELAPAGGPPGETPAGAEAPVAAPATALGDEGQSRPVNEGGGDVRGMASAPSFGTMLRKRQTAVAARHKVAIRIARLEALAATRGAVAAARCGALLLVGRDVRPLRRGWCLVEAAEALRAKGPEAITVLPVDKAFTRGDLVRQLRELDVAEVVAEAEEHDKEVLAEVLGVPLPQPPAAPAPGGPANTGASIAASSDPAGQGRRRTSAADEQHPHPHSHPHPHTHSSTHPHHRASGSHPRFSRATGGGSGSGSGCATGLDPGEDDGYSGGRALARQLQLALLLRPMDGALPARMLGTPMGDAQPVATNSTARNSSSGPTAFATRAAVHAAAAAKASSSKVGTFALPGSACSFDWLWDWTPLTSWLALPPEHPSWQALLITGTHGSGKTTAMAALLSRLMAATTTIAVTRGSLAEAPLVACHFCSAADARSLVATEVVRSLAYQLSCRSPGFGAAVCQSLLQLGGAGGGLALTPDGWLQGLEETVAALLRHPMEQDAEARAARGDAPIHYLLVLDGLDEGPASAASSSVLMDSFTAEDTGPSACPGHYQVLRLLVDHLRHLPGVRLVLSCTEAADASSSAASGSGTGSAAVLPWRPFDLPPPLIVAPRIAADASTSVLLAVRQAFRPRELPLTSLRRDKHLPLALAHALQEYVLSPELTARELLWQARGCLAHVHILQLLVKHAAEQLGPAAGLPDGTVIAAAATAANAAVGGAAPPARGAGERPLAAATDAPTAAVLQALQRADMPLPEEPQRPLPPQPGVEPPQAQHQEQQRGSELPSRPRTPPRQDQAGFARQQSLRRPNGAASALDVVMENEAPGEAGVCLMLTEPQLPDFPSLAAPPTELAALNSPNTGDGTLGVHIIPPVSRGSLADEYGVLWHVRTAELQEAAMIALARGAEAAAEESPPSPQQNRTSPRRMDTMMMGLTAAQPPAPPYPPERAARLQLLVDVLAAAQEPLEWALLVCVMLAAEVPAGPSPVAAAPTIPAGGTASTPVRTHRADVRSPSPAALGDAAAAPGSPSDTRSRRESTPVSLRSGAGGDVGRLQQSRTPSRSEVEDVSRSVSPGVGVASQPVSSRAEEAAVGASALRDLEWALQEGPLAGLVWVSGCGRVTVRHSSFLDWLRRVSTVASASAATGGPTTAAAPAPAAAVAVPAVEAVGVATAVAEAPLSMPRAQRGHTVLARLLRRQLLSGRLLPAAAASAAAGAPLAAGVGGLRRPGSASNVLLSSLEAAGSASAVALGMMKPAQLSTGLGLGFGGDGGINGAGGSLGPPASMYCLRHVVTHCAEGRLPTSELETTIMDLDFWRQVLEGFHLEMVAASLEHLLRTRGPHSRRLIPPQQQQVLLQQQGTLPLGMLPRTPPPAGAAAALVRGTPAVTPSPQGSVSNANANASGGGSPAIGGATAGAVVVSGLLRDGRTVVPPPLPAATFSLLRDTLLWLLQDGRSLMRRPEPLMLACSASCTPLQSPFRRLAEQITTLAAAAAAAAAADSLFPPPPKLGAMSAAPLATPSPALVLTGGAAAAVMEPVFPPSFVTAMPHPRFRRPIMRATECPHRVAGLLASPDGELVLATAECASKASVLDTLSGRRALLLHAGRCAHHSALSAAAFAPSRALLATAGSIPGSVTLWDLTTGAPRATLQLRSPEPRSGPGDTHVDADAAAQAARPSLVLTSAIPSLASALPSAGPSLAISTVYPAPTQRGHPSSQHAVSIAALGEGHARRDPSAGPDVPRGSSTMTHASQPSLAFSSDDPYVTCIAVYEPGPAASATAYGAPMGARVVQSSAAAAASAAEGNGVAIVRGSALVAAGDSSSGRVVLWAVAPDGWGASRTAELVPPTGVSALHGGGGVAWLGFLPGGKSLLVVQSCSTTLRVWNVQSHNITATLHLPAACGEIFGVHIAPKCAADTAAGAATAAATLIAAVCTSSTELRALDGNRPLASLPFGAGGEGGGRNWADNCVAFSPAGGLLATAGAMYGKQLSTAGSCGATSRGGTPAVTATRAVAENAQQLVADHCAATLWDARSGAQVTQLRGMRSAIRALAFSPDGALLAAAVADAAVVLWSAATGACLSVLAMAEEVMDVPAVVFARQGAVLMAAGGSAVYTWDVQLAVAAAGTDAAAACVTGSGFASGTPASRNRTPLGGGGAVGGGSRRAMNPSSDVCGHRDRVAAVCFSPNGALALTGGDDSTIRLWDVVRHTELVEVEQPGAVLALAWSPDGTLIAAGLSNGTAHVWYFGAVTAASMDTAPGAAIAAAAAAIGDFTTREAARLRCSGSGGDEVDDVSTTMNAVSFSADGKLFLTGNGEGRVSVWSVRDWQQVSTALPSVRPSRTAISRGAPSADGDGGAAGSSVFGSGTMVGRYAGASFSADGRWVLMGCGREVMLWSVKSKRESSGRLGPHGAPVVAVVASPCGKLAAVGAGSSVTIWGRPDAIVGGTGGGAAAGGVGGGTSRTSGAVSFRTNPSDGAGGGPASPFSARVTLLPALAEVTGLAFSADGTMLAASLGDGSVRVWDTGRWKEVGWLSAHGAAATSVVFSPNGMLLLSAGADSRCLAWSTRDVLQPVFGSAAGALEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ85</accession>
    <name>A0ABQ5RQ85_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001567</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="90"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="165"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="283"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="473"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="542"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="564"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="581"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="593"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="619"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="670" mass="71203" checksum="21D758A0B425826D" modified="2025-10-08" version="1" fragment="single">PKLTRNVTIVMSDRAEPRDREPAPVPKADSKDDRDRDRDRDRDRDRDRDRDRDRELAGKEYYPTKDYAKDFPPREYPPRDYSRTDITYHNKIYVGGLDFSATKEDIAEVFTKAGKVVNVQLVVDHETQRSKGYAFVTFADEDSYEYAIKRMNGALVRGKEVKVNKALNPEEKADRGPPRDRDPPGGRYPPSRPYHSDKPHFEKPPGPKMKGFRVTISGLPSHYTWRELKDFLRNTTAAQPTYANVERPGLGVGEWATSEERRKAIERIDGIRLEGGELRCVPLDEDRGGDPGHGAGGSYYRSGGGHDDAGYSRGERGRGGGFDDRYYPGPYEGTRASGYEDRYPDKYSSGRGEGPGPRAGGYEGGGNASRYENYGARDGGGRDPGGGYGREAPSAGYNSRDAPTYNSREPGYSSRDPPGYAARDGAGYGPSGGSSGYGSRGGGAYPPSSGYNSRDPAPRGGGGYDDRYTDKYGSGGRGDYPAGGAGGSRPGGYDDRGARYDAGGYDASRREPPYAPPLKDPYAGRDRDGGYGPRDVPYSGSRGSGGAGSGGGGRDAGYDRRYEGGAGGGYERVGAGGGSSGYDRGYGYEPAVAGGGGGGYKPGGSAAPGGYGAPARGPVPGGTIGHAGRSGSGDRGGGYPPSGYEGSGRGGPDRSYPSSRSGGYDRSAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQL6</accession>
    <name>A0ABQ5RQL6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001863</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="235" mass="26108" checksum="173A228D9B8142F6" modified="2025-10-08" version="1" fragment="single">MAQQRRMPAHGPTAPVLGEGTDNSSITAEDVCLRDQRAAGLRFMKAFGVDVANDELADALSEATKAEVEYGGVPLGNGATLQQLQSHVQQLLQGQGGLAHSFQLMQQQITLVEQQSQQQMGLLEQQSQQQMRLLEQLMQQQMELQRQMGLLQRQMGLLQGQMGLLQRQMGQVQQQLKEILQQQQHGLSSQQQQGTQQEQGIQQPPQEQQQSVLLPLPQQQQGIQQPPSSSSSNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQV5</accession>
    <name>A0ABQ5RQV5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001982</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.67" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF81">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND DUAL-SPECIFICITY PROTEIN PHOSPHATASE PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="5">
        <location>
            <begin position="14"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="99"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="269"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="436"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="309"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="655" mass="71479" checksum="FE89B194D6773BE0" modified="2025-10-08" version="1">MAFILRRLISTNKRRYQQDGYDLDLSYITPRIIAMGFPCDGISSLYRNPVNDVVRLLEARHTGAYKVYNLCVERGYDPRVFKGRVMRVPMYDGQAPPLHVILEVCRDAVAWLAADPAHVVVVHCKAGKGRTGAVVCALLLAMDPELGPASLDQTLTLWAERRTRDSKGLSIPSQRRFVGYCHQLLLAARRKCYDQQSICNHSVCTAAATRPTCAQSSKPGGSCAEDAGADAGEYRAGDRERLVNGNNHNFNSHNISVRGDEASCFTAESGRRSQRQVTGDANFDSCTRTDLKSNPELQTGSCNPQHVPRSSQSRGQSSESRSQQQHNVTTQQQPSQQRPQPQSELEGRVPRSLQEALTEADVPQHPVKLLRMCFRGLPHWLLRDCTASVWWRPVGHHEAHPVCHVRVRPGGSWAGCYNPDGAVHSAGDHAGTSAGVTWALRPQPNGDSSSGRVGDSARGNGNGNVYGKGQRSRCVRELVLDLTGLPQLSPPPPPSVLPYYVSSVRSDYLKPLDAPADSTVPVSVTASTTASTVMPGARDQTLLGLGNVWKGYQQLSVVASSSTSANAQTRSYPGQGGPVVSGDVKIQMFRGSIADPPHDVFKGSVHQFSAWICSSFLDPRASSLELSCQELDKLSKRLRRRRGSPCIVLEYEALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR29</accession>
    <name>A0ABQ5RR29_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002097</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI60032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF46">
        <property type="entry name" value="LEUCINE RICH REPEAT-CONTAINING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="5">
        <location>
            <begin position="152"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="621"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="783" mass="81370" checksum="C973811E4F76B029" modified="2025-10-08" version="1" fragment="single">RPGDPRDGLPQVPRSPGDVGSQARAAHRLQTITRSNYSRTSWRTAILSEQEDSTNAEHISLQAQPTVQDSAGKSFAKEGGSGGAAQTDEVEVADNGTLTGAELRVKLALAASSGRLDLTDCRLEKLPTEVLQLTELEELQLSGNCLTDLPDEISRLTALRRLGLAGNLLTTLPPGIGALKGLEGLWLHGNLIASLPQQLSNLGALRALSLAGNCLEGVPSGSLAGLTSLTDLTLAGNRLEALPPGELAPLSRLRKLALNGNRLMSRSPGELGLGSNMTGLTELMMQGNRLECADPAIFECPALQELSLADNQLTGLPSSLAAATALSRLHLFGNCITSLTAAQLAGLPSLSSCWLEGNALRGEAVAALIAVAGSGAMPHLKALGLDQSQVEAAAAAATGQPGDGGAAIQLPSNVRVGCVLGSGPGYFKLQYGPQCPAGSSGSDRPGARRAGWSAERGGTGVGCGHDLLVVAFGSAPGTPNWGGLLSKVYTTAGESRYFDVLYVADPSRDWYGGGDDSVYAYYRDRLSAYTCNYARVLMLGDSMGATAALMFSDLATGALAFCPQVDLTAASIRPGRPGPWLSRLRQRLVAAVTASKGHLNVLVGTWQHDLGQANLLLARSASEPQTRRDSAGNGRNVEETIVNKNGPGARQGVSRGWREAGVATMNEGGVRVRMVGSDSGGAAPQRKPWAWERTVESTGMSIGVDAAGCGTCVQDGDLRSERHGSASYGDSGCGGVNIKVFSVDSHRLAAALDARGQLVPLVQDAVLHQLGLPLRNVRVSNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR75</accession>
    <name>A0ABQ5RR75_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_001693</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="149"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="335" mass="34766" checksum="0B9F7475BF488784" modified="2025-10-08" version="1" fragment="single">DTVNTASRMESTGLPGSIHASEAARALLRNEVWVPTGGIEVKGRGLMQTYLWSPPDAKMVKAEVAGSKSSMEWRATISSTGNPHGWEKRWSQRQSQRHSHRLRNVQAKAGAAGLTNPRPSRSCSCSCSCSCSCSCSAMQHSHTQVQAHSHTQIQAQSHTQVQAHSHTQIQAQSHTQIQAQSHTQIQAQSQSHILKVSRLHGDSTCYVGANGNSTFGSSTATASAGPAPASASTDASASKGMDAQLLALLSPALLPSPCWDGAGAAAAGASGSSSSADDDSGSDSVSLSGADSCVALAALLLESGICEGPLRAPVVPHTGGLQRLEQRDREARSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSD2</accession>
    <name>A0ABQ5RSD2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="7"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="396"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="453" mass="47927" checksum="CCB7C7363A9917E7" modified="2025-10-08" version="1">MDPIHVLRGHLGDVQSLSYLSEDHLYAGDSRGDLRLWDLASCRTEYTARLHDANSGILHLACLPDYGILLSQGRDGCVKLWAAGRTCALDRPTPLQEIRTGSFNFCRCAVWAPALDSPGGTSGPQALDSRPAAAAAAVGPAHSLVSGLAGDPRDLLVACAGHDPADVNVWRPAEAPSISTRLRLSEGINSGAETNHGMCMALAFLSPSPAPSHCETGMGTSESSRSDLEPLDEQDISTCGGGDVDSGGNKGAASDPRFIIAGYEDGVVALWDLRKPGRPLGSLRAHTEPVMCLDVRARVQGRSKGKGGAALQAGRENDDATELYDLVSGSADDKISCCEVRPGAPQPVTLTRQVQLREGGTSDVRIRRDGKLFACGCWDGRVRLFSVRRREPLAVLKYHRSHVTAVAFSLETNQLASASRDGTIALWSVYSSTSAATTALRQRESSSVSAGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSJ6</accession>
    <name>A0ABQ5RSJ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Haloacid dehalogenase-like hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002584</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07516">
        <property type="entry name" value="HAD_Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000:SF8">
        <property type="entry name" value="HAD SUPERFAMILY HYDROLASE-LIKE, TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01144">
        <property type="entry name" value="C2.B.4:_PGP_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01228">
        <property type="entry name" value="COF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="36657" checksum="1247786C4DFB31B6" modified="2025-10-08" version="1">MRCTIKDAHISRLKLSWNERHTIPGCSMPNPAGYLSRSSRWPPMQSSQTDCRPVSSKRTVTKATSGAEIKLIASDVDGTLLNHCQQLTPAVEAAVKQASALGVPLVVATGKARGPWAHEVLPRLGLTTPGVFLQGLLVYDADGNRLYEQQLPVDVARDCISLAKERGITLTAYCGERILCAATDSHTDRLNFYKEPPPEAVGDLALIVGEIQIQKLIFMADQHVIDSLRPQVELLLTGRASLTTALSGMLEVLPLGASKGAGLSWLLEHLGVDPAHVMALGDGENDVEMLQMAGMGVAMGNAAPKAKSAADVVLEETNDEDGVAKAIIRYVLEPRLVAVQGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSM2</accession>
    <name>A0ABQ5RSM2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease Z</fullName>
            <ecNumber evidence="4">3.1.26.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_002748</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase Z family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07718">
        <property type="entry name" value="RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027794">
        <property type="entry name" value="tRNase_Z_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13691">
        <property type="entry name" value="Lactamase_B_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="12">
        <location>
            <begin position="705"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="31"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="225"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="470"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="641"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI60588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="991" mass="105815" checksum="29A64876675FAB4D" modified="2025-10-08" version="1">MFHYRCLFQRSCRPCLHSSFLRVATEQRMGKVKAQTQVTPQTKQDGKRNQNQSQAQPQTQQQRNERSAGGMRHYSSYLQVLALDIDHTSPSVLLFFNSERYLFNAGEGIQRLFREHKIKISQINSYFITRVSTETLGGMPGMALSVTPPDARGLLGKQAACTVKGPPGLASYVAAFRGYINYENTVAVEEFEHSTSEPVLKTDLVSITPILVRVTAWKTQTAAIADGDGDADGPAAKRQRTDAAGKTPAQVAEQDLQRPADLSEPDVAVYAIQMAGRPGKFMPQRALELGVPQGKLFGELQRGLSVTTQSGRVVTPDDVMEPAMPGPSVLLLDTPSLDSLRAVAADERVRRFMDEAVASGGEDAAAVAANNRVCVVVHLIPAALVANEDAMAEWRALMGPTWRHVVVSSGPHQPSSISRATTFQAKLHALDPTCFPLFALENAAAPPSVPQAELEAAAAVSREAALKEAAAATSRSGGKRTGSKADGSREKAGAAAAAAAAAAAPEIAEPPADGKGKQGQAPVHVVQQLPSVLPLEPGCQIRAISAVRFNLVPLNRQGLEYTDVFKYDSSSKILDDIRSNSAYAPILAAAAEVRMPTATSLETASQLAVADGAEPEGAPECGCDSGMANGVKAEATGIKAAGGTDLDSDGRRGAPSAESVAGAAEARPATSPDQPIPEPLTSGDRRLAELTFLGTASSQPSKYRNVSGCYVDLFEAGGLLVDCGEDAMGQLKRRFGNADAEHRLLNQLTCVWVSHMHADHHGGLYRLLEWRARRGAAPLLVIGPYQLFNVLVRYSAVVPIQFIFLPNAALSSGRIEGNGQLPAVTTAAYQGAKDRLGLAALKPFPVDHIKDAHGLVLEGRAGWRLVFSGDTRPCQKTVDAARGATLLVHEATFEESMQAEARAKRHSTTAEAVGVGEKAGVYRTVLTHFSTRYPTLPELDLSRHPRVAVAMDLMSINLADLPWLPRLVRPMGMLFKQLEAEKVSGGDDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSS7</accession>
    <name>A0ABQ5RSS7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="280" mass="31193" checksum="AE5B1948CA005892" modified="2025-10-08" version="1" fragment="single">TFRLTCDQSLAIAGLDSRRMRHIISMLFQAVALLLLARMASAQKYNLTGSIKAVGLVASNEPPGVDCDKPGICSFAVQTIPARNLWYWCGFKTTHKTIYNVTSINNVNITMYMMRQSQLQGCVLPNVTFVAENCLTIPESSCNTNATCNMRLFGLVFPDESCFVIINNKAKTLQVSIKINNFFDRPRYYGTVFVIFFSVVGGLAIISNVAWQFYSIIIAPVNRHHEPIKVKREDPIKKDVESVSSITSATTSSAAAVRIGWYQRIIAKLRPMPEVEGHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSS9</accession>
    <name>A0ABQ5RSS9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="135" mass="14962" checksum="50494658B5E96D75" modified="2025-10-08" version="1">MMTLINVSPVKRSFNFCKSVLDRQLGVDHRPPQKHHHVLHCWRQCCQTCLRLMQEAGWQDSVLAATCPRVRHQQRHGGPKVQQRAAGGPAPVSVTGEPVQRSVAGGDQDMCGVGQVGGTWVCNANRRRDGESETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RST8</accession>
    <name>A0ABQ5RST8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="545"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="474"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="926" mass="95574" checksum="DC3B5A65E42C9D47" modified="2025-10-08" version="1" fragment="single">TMNILACFGRARPASTTSKRQLRQPSWSVARLDEHDCIVQTTTQSVQAASEVQQEADPCSESPFRSGNIAPIAEDEGQYQTLNKQELAPELFMFHASSSSNKSTPYSHHGLTTSNPLANDHLEGHLLPAGLDLATLHHLIDSVYVSSLNHHNHTQESHGSLQGPRVNGGLMMPGGGGGSGGQERSITLSLQQRQQLQQVLPEVRDAPDLRSLTYAQLVRLCQPEDIVSDLAGLKLIGTGRRSVTCQARWRGAHVAVKLTSSLSLDPNTSAIIKQALVSKALAHPNVLQHYSVRCCRFALSPIQAAVAVVAAVVASLPHGHCTGNPSAAGQTAAGATTAAVNALDCATVSSASAPGSRAAVAEECDANGGGGDSTTSTAAAAAAAVVHHLSGPTATNNVVQNDSASTGVDSAAEQISDLIVGTYGGSATSLPPPSSLLSGCQIGLGYGHSKAGAGGSRVLKAPREQFSRTIQDPAASQVATGTAAAPPEPARRQGCATREQQQPLSHVECRGTAETASPRTHPAAETGRSAAAAEASTFATPSHIPAEEAEVGAGATAMSAAATAVKGTAASVSHYNAATAAATTRKSAGTHGAAAVAVGPGTVSPGSLSFNSYEGFGDPFGSLRHALTLDQIAALLSAAEGDYLTVIIMEYADKSTLQQAVQRGLFVPNRVWNGRVALRALLRTSLELACALQHLHSRGVVHGSLRPANVLLKSSNIDRRGFTAKVSNFSLARVCFGDYDDLVLEPVPLGLLRGNSNVAMAAPEAAGAGAAENGRSLEPRRSSTGGVDVAALPFWSPEQLCGVVGKASDVYAFGVTLYFMSTGSLPYAGIPTEEVVRGVAAGELRPRWPDDDGDNGGVVTAAGAAGPEAAAVPSLARSPLRDLCALCWQPDPRARPTFAQVCVSLQAIEHAVRQQLRKQVPTTGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSV7</accession>
    <name>A0ABQ5RSV7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="20"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="56"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="161" mass="18019" checksum="8F72481B69FF50F0" modified="2025-10-08" version="1">MPNKAREGPYSTNLPLLNEDELEMCRKAFNMFDKESSGTIDTKDLRTALSALGQNPSEEEMFVMISQVDEEGSRCIEFSEFVRVIQFNKALSARDADELDTLDAFAALGGNIDRTGKISIDKLKAICEEFELTINLDRLAKDAEHEINGTLTFEEFKAVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTH0</accession>
    <name>A0ABQ5RTH0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003133</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15848">
        <property type="entry name" value="SNARE_syntaxin1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF307">
        <property type="entry name" value="PROTEIN SSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="8">
        <location>
            <begin position="109"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="227" mass="25320" checksum="82086C0654FA3EB7" modified="2025-10-08" version="1">MGVVWGARHLAPVLCAQSPIGCVAREYCLVPSCNVRNIGTCAPAPKVIINKVIKLAHAVMANIEALDKENEANKESEEQGQGTASERMRTSQTACLKIKLKDHMREFSYLFQSEYQQASSNLEQSLSELHQIFLDMTVLVEAQGEMLDNIEKQVARSVDYVKGGTEALQDAKQLQKRQSQQSPVSRGLPMPTSPKTVYFMYRRVYERTARCKNAVGTANSREDYGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU65</accession>
    <name>A0ABQ5RU65_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003265</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.390.10">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051644">
        <property type="entry name" value="TRAMP_AT-DNA-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543:SF2">
        <property type="entry name" value="AGAP013096-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="32"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="268"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="496"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="575"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="798"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="930"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1055"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1144"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1211"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1263"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1306"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1400"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="321"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="496"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="600"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="626"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="659"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="817"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1078"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1147"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1214"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1265"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1412"/>
            <end position="1421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1421" mass="145262" checksum="1CBBAA0E2870A27E" modified="2025-10-08" version="1">MLRDAAFETELPVPLCGRLPTTTISECGEGDCSNFESSETDAAGEQPSFERPAYSSFTSQHDDCSRGDMNARGQKGNAIFGGGSAFANTTGTLSDDSDDERNARTAKPTRPQHPQPYSQPLYECNSMQELLSILMAPSGGGNQLTVRPSTAGNALDGTRGDHIIYQINQQQQQSSEEPTLQNIDSNNLPDLAGLLQTLGSRSRPYGIGLVPAEVIRAQLFFSSLAAQTMVEGIGNDNGMNGRSAGRDFESAVAAAANSAAYLFARNTSPGEHLPQQTGCSPAPRASVPDMPSNGTPFNTTGGCGSHAQVSPEQSGLPPRPSSRQQAQPKQQQQQQQAAPAPSDPSRPMLVLPLVDTERAAPPPQPMQQQQQVRLHASDHLGGPNGPPLPVGKCLAPAGSVRPSMPDLGPPLETHPSFTGNNDELIAAMAAAGLGPKMSSVATPTCPVEGAEGRPTAAPTGTSPTTAAVAAAMMAFPASAAGIVSAGRTFTAAAAVRAEPARPQDATSGPTTMTTTGVVFTGPPQLQPQPPSSGGPGPRQRRYEVHRARGSSSGMAEFPDETALAAIAGAVASFPDSVSGSAPGTPPVSPHGNATRPPQPWSASSSARSLQSYNISQAPPTQPQQPPSQQQQQSTPEQLQQQNNIQQQQQVPSLWPTPPSSYIYTPSPSPGPSSSSPLRRHRTPSLGGGYPVERSVPTASATPAISVADGIAIVPSPTDTCAGPAARPLAAEHLASPFALVVPNRSLVFSSGSGGNHGGGGGSALGGGTSAAGVIVGVGGEGGRTSADFVRRIEHQAFPNAPADNQNGNQHHDRISSHPLTSIYSRDTPSGALSNAECSSRGVARLSLAEVNGGHPGPLASLSRGFSVSGGEGSISGCGLSALTLGNSTGTGSVGCVGSGSIGTMAPHKRLRGPPPTASHLKAVGSAGASVTAEDDCGGSSGGEGDEGGNATTTTADKTWSAEEIPGPVAVLVTGFSREVGEDGRRRRITVQGVFHADRYLANQDCIQYENKFISRSAFEKAGGSTTAKWHCSIKIAGTQSVTLGNWLNSKGLPVLKGGSRKSKRQRAVERSVAPVGSSPPQIQLTSQQLQQQPQLPPRLSMGGSIPGALLPNVSAVVAGSPRNSVGPVGVSVSGACVMSGVSLGDWSHPQLQPQQQQQHQPQPASQPQPSARQQVSQQVLPYLSQQPQPQGPGVVRSGVVATGQRLPICPPVIVPQTPIQSPQQQQTSPSPSGGVPLPPMPSPQHSGQVYPPSVFHLAQLSPPVTGMAAPTPTSTPTPSGGGAGGFARCMSQVGPDGRPFSCPVGMSRAESGVSSLGPAPMSAPQPGSNPYPHQHQTSSPFTAAMNVPGGSGGGAPVVTGADRPMYGTCNAGVFGLGHPRSTSGGASPLVYASSNASSSAARVASRPTLPYMLPPLQPQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUK7</accession>
    <name>A0ABQ5RUK7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="296" mass="32781" checksum="CFE9A405E9A63129" modified="2025-10-08" version="1" fragment="single">LRIAEQDLSWYQDVFAVPYVTNLLQFYTGPKAPSNKLAELQAKRLNVHLQHDIPRTPGFQRQHHAISIEALKEVKPRVRDYIRHGGEAWAKRLYDELTTGPMFVQNVNRISAAAGQPHAMDAVNAFSTLLTALQPTGELARQYYNAIFTGVLVANITNTRPSLQSRETLEAWLPDILKALLLKMANGDIDNAPVSAAQAQDAYNEMIEGAAFVSAVANVFMAINNCKLVKFARLAEDGFSTVAREYPRLAFLGKSLLLVGWVYGLINTILTLVHGGMNQMDGLRKAQFATQCVQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUN2</accession>
    <name>A0ABQ5RUN2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003438</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051887">
        <property type="entry name" value="GH18_Domain-Containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46290">
        <property type="entry name" value="DI-N-ACETYLCHITOBIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46290:SF1">
        <property type="entry name" value="DI-N-ACETYLCHITOBIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="3">
        <location>
            <begin position="1"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="393" mass="44126" checksum="D36018B951150C70" modified="2025-10-08" version="1">MVAFIHCIVNGLQYNSGEDGAVRPQIPCPCERRELCNFIQSEREREFLGFFYPYNQQIALYDWTQITDLAWVPPDRTDVICAAHAAGVRVLLNADDVSGSIVDVHVRREWIARQVELARSYWYDGINFDYELPAKMDSDLSRNYTQLVRDTSAAFKKLNPFAKVSVDVPWSAFGNDDRDYDYVGLAAVADRLFVMSYDMQAQIFGRCAAAANSPQPQILKGVRQYLDLGIPASKLILGVAWYGYDYPCERDAHGVAPDPWVDVCLLRPVEYRGSLCSDAAGVQRDHKDLMALLRSGRNTTGLIWDPLAGSPRFNYVQNEESGGAVHQVWLETPESLTLRYALARKLGLRGVGAWNMGCLEHSGDAEPAARADTAAMWQAVRNFTGGPNRWEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUU0</accession>
    <name>A0ABQ5RUU0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003339</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007307">
        <property type="entry name" value="Ltv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531">
        <property type="entry name" value="LOW-TEMPERATURE VIABILITY PROTEIN LTV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531:SF0">
        <property type="entry name" value="PROTEIN LTV1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04180">
        <property type="entry name" value="LTV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="117"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="221" mass="23496" checksum="0D9A1A1018920993" modified="2025-10-08" version="1" fragment="single">MGKKKAFIDKKKAVTYSLVYRDPSEDPIGEDGGQRVLAPVGGARGPHPPSEDYYEDGYYEDEASIYDSEYSLSMSRSATAHHDASYILSEERRRELLALGFPDDGYDYLKHMRDLGRGKVPPTAAGRGGVATIPEDQELDGFDETGEDDDVDQWRDEGPGSDEDFTIYDEPEAGTGGGAGAVEEARQGKKLDAAVLAAAAAFAEGTGPSTSTSGGGGRTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUX3</accession>
    <name>A0ABQ5RUX3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003787</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058920">
        <property type="entry name" value="PAP-OAS1-bd-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058921">
        <property type="entry name" value="PAP/OAS1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45979:SF30">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45979">
        <property type="entry name" value="PAP/OAS1 SUBSTRATE-BINDING DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26180">
        <property type="entry name" value="PAP-OAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="35"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="PAP/OAS1 substrate-binding-related" evidence="3">
        <location>
            <begin position="185"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="733"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="812"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="894"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="935"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1140"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1308"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1426"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1592"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2014"/>
            <end position="2033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2169"/>
            <end position="2255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2269"/>
            <end position="2288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2326"/>
            <end position="2347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2414"/>
            <end position="2462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2734"/>
            <end position="2864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2900"/>
            <end position="2942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="434"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="816"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="832"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="846"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="894"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="938"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="994"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1152"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1351"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1457"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1595"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1617"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2021"/>
            <end position="2031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2197"/>
            <end position="2220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2221"/>
            <end position="2232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2243"/>
            <end position="2255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2276"/>
            <end position="2287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2326"/>
            <end position="2336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2429"/>
            <end position="2448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2751"/>
            <end position="2762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2776"/>
            <end position="2792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2821"/>
            <end position="2839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2840"/>
            <end position="2849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2854"/>
            <end position="2864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2900"/>
            <end position="2914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2921"/>
            <end position="2936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2942" mass="293474" checksum="AE7E915CCF5C12C8" modified="2025-10-08" version="1">MALAQQSPNQPYSSNPKSLKNLQPKFADELLARTDTLISRIRPTGLSLQRRWIITDHVTAIVRRCFAPHDVTAIPFGSVPLKTYLPDGDIDLSIYSESPRAQALKEALRDTWATQLQICLEDEANNPTAVFRVANVQVIHAEVKLLKCLVDNIVVDISFFQVGGLNTYNFLEDVDRFVDQCIPVRKHLFKDSIILVKGWCYYESRVLGAHHGLISTYALETLVLYVINLYHRELVNPLQVLYKFLVECSGFDWENYCLSLEGPIPLSTFPRPVVETPEALQRDALLTKDFMARAYFKYTEPQLKAQGGEPKQFAIKQLNVMDPILPGNNLGRSVSKASYLRIRRAFEHGARMLAEITEQAKELGPFIGAKRFDNFFGKVWNAQRPRNRPAAAAGEHGNVMPLGAPPGLHHPVALRPLPHPFPNVPAPPPPSTSAPPIAGSSPARVSGGGPSVAAVYGGGPSGTGSDGGASRNGMPQLTYDNIAGDLHMVRRQEQGQGPRPCGPAGSGMTAGPQWILDAGQDAGATSQDGAPVPTAAGAAVSDADSFLRNYILRQIASNLRGASGGVTATCGAALPSVVPQAWDSPLAILGEDTNPSLDPQTPRIMALLSPHLLSQQQQAQASSSSTTVAGVPWGLQTAPEQQGALPVGSASALQTEMEDATGEGSKMPRVMSAHVIPGRSSGLTASGGVMAVSNVVAAGPPTAAAAASAGGTHATVARPTGCTAAATIHVGSVCTGPSDEDDKEAIGQEQGRRIGDGEEVPPAPLPTPLPDDSAALGTFGQGPSDTLEDDAAASEGLVASPDTLVCVGLADKSSSDVEDMDEGDENGLAESSGRRESEQLREEGSRSVSRKTSSASIAQPPGAGPSAAAPLYRAQPSAAAVLVPAVATAVPSAVPAQTSGSGSGGNAAIANSGEGVSPRQLPYAVPVMPEELQQKLMSRSPSSGPVTSTSTAAPAAPSASPPGRIVTGGASSLFQHSGSGAQHGSGPGSSGACTPPPPHPPPPLSQLDAVAAAAASAAALQAMFHSSGGGSAGGASGVGGGHMHRHSPVGRGGRSVPVGGAPTAMPYMGGMLPPAHWSTGAGLAAMSPEAQSFAQLVHAAAYGRQPGGPPPPPLLGGALLHPHSHLLHAFHGGPTAAYFPLTMAPPPPPPSSSMSPSQQQQQVESSLLAAATAAAGMPSYALHPHSLHLSHGLSAQHALQQQQHLLATETAAAAAQRRFSHDGSSAGSSGGGACGSGISSGMYLGRQRTPSAVFLGGSAASAAAATSDQQHLQQFFQQHFQQLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQPPSTMVRANSRGGGSSAAAFRRGILSGDSPTGSMPLAPPPPPPPASGGTQHRTAAAAAMAYGGYGGAEGVGVHLSAAPNVGRKENPPETLATLQQVSAQLLTLQQQSSSALWARDADDESTSSADAGAVTSGGVAEPVSGVADRDQGGRQDGEPVLRQGNGAALTGPSAGLWAGSEHVISAAARSKLSISSQAAHGGDAVPHGCGSGTDLASYSTAATLVTSKAAGAGADTSATLAAVAGAAVAAVADAQAAESAATLSGVSTLLAPFPSFEMPLQIALVPPPPPPAQHQGGPPPVTGPSGGTLSSGPVNQQQLQQWLHSVQPPSFMFNPMPRSVPPPGSASVAAAAAAVVAAATSGGHRQGAAVPAPPPPGPGVCSNTATSGTAVVVPNVPGPPSSGSGASTRAGSMTGFPPTMRMPGGSMGLPLSAGVGSHQQTSNTPTTAAAALAGASSLMGQGLLTHPHPAATIHYNRLSGYAHEYMLGVSPPSPHLGSSLQPPLPPPAIANFQHSPPSLLSMPYLYDMKATAAAIGGPEARWAYGSASSGQSPSQSFGSSAGLYGVGGGKPTTLLPPPPPPPVTAHLPSGSSAGGAIGFHGPAATPQPGSTLAAIGATLLRDPAPPPFTAAQVFAALPLHAAGLSHAHAHTRDPHAMNLELTRKIVASMQPLLSGGLQEVGNLANTGSGGMVAECGSGGDMSVAPGSNNANGGGGGGGGLSGEASGALQLDELMFEVEAGGSHPTSTAQPRLQYNAGKGSAASCVSGAEPVVGLVTATTPSCMVSGIGIRADDVAQTAPSPTSGDGETVLNGLLSSLPAGSMARAIVLPEPAGAPGSFVSGVAAPRAPHPLLGHACAETRRSPLPGTKIPSTVSDGGDALEIDSGEQQQPRAQTQQQHQRPSQRSADADAAPPPPPLPPPAFHGPNLGGGTTTTGESSNAAANTSAAVAPTSAAVAPHASSSVQLGSGGSASSGGGAQEVLHQLTPPASVSSSGSTPLSHSPQAAVLVITSQTGAPLGQVESNDSANPGAGGGGAIPVVTLQPTPAGATIVQPTCPHMVMRASGTIDGAPTAAAVGTPTVEAVRLPPVSLMSLTMPLQILSELTPPQQQQQQSGDAGMSSPLFPSSQQQSTPGLSTSGLPPPPPFGTALLGVPRVPSQRYDPLGAAVAAAAAARRTTSTGTASAPSPSSPAVGPAAAYSLSQQAFPMAIAVTLPTPAAAASLSPPHAMDAVTVAAVSPPMTAMARAANESLVSSPVAVLPVPGSGLQSGSASHQQRAPPASAASTQALLAVAMCAGTQQRDGSPRQVELPPGLVIAPSGGQHPAAAAAAAAGYIRPVTSVFATQRLGAGDGPAAAEVVAAAAVEGPPTPSAAAPAAELYATRRAGVFASTEVAATAAVPGVDVLSDTALMMAVDQRLAQGQGAALESHGSLAKGQPGAHGASTSLPTGTADNRSVVPPAAPAATASGTNQGPLAAAQGSQSLAGFAAPYSGTQTPRKAAPYRPPHHPQPQPQQHASQPHLFSPTAVPAASQPPPSPASPVPGARTGGGNGGAAATGAAGNNGYGGSSTTAARQAASQAVASRGGAASRSEVAAAVAAAAAAVAAPPLQAGSIRNTPGATSAKNNGAQVGPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV63</accession>
    <name>A0ABQ5RV63_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="163"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="422" mass="47105" checksum="87E6F406782F0128" modified="2025-10-08" version="1">MFRSSCFKTDVEVDVYHAPGIMGHILSLEALEGSFGPNVVPQFSGVLPHVEIGGAPSIELEKIDGLFYAEDSSYDSWMGRYGSNRCLSGKKVREELRKVKLAITKQDSNLGVLWHKRYGHLGFDALAKVAGSDMVTGFGASVDDVVAAKGQFCKPCAEGKQTRQPHPSTGNVTSKRLDLVHMDLCGPLPVKSWNNAKYWVTFYDDCTKFSEVRCITGKTVVPETVKEVIKLWERQTGDKVRRIHTDRGLEYVNEELASTLRDMGIFHEKTAPYTPQQNGAAERLDRTLEEYVRTMLADSGLPQEMWSEAVITANTLRNVSPTKGSNLTPWERFIGEKPDVSKLRVFGCSAYVMIPKQQRHKLDPVSVPGQFVGYAPGMSAWRVWIPKDRRVRISCDLKFDEWGVRGSMEFDASGSDPESGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWC3</accession>
    <name>A0ABQ5RWC3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027850">
        <property type="entry name" value="DUF4504"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31366">
        <property type="entry name" value="UPF0739 PROTEIN C1ORF74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31366:SF2">
        <property type="entry name" value="UPF0739 PROTEIN C1ORF74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="506" mass="54431" checksum="FC474ADCF56C5CB6" modified="2025-10-08" version="1">MQHGRDDTNPEPKTRSSTPFLNSVRDGAVCSLTVQHRWRVAERSSGRRTPFLQVHCEMDAAAAGHVVAAVRAQACRSAAVRRVAARLSYDVIVVLCGVRPAFLLDYAVVPPEPVLAVAQAGAEAVGTAVAVLTLDGCHLVGRVDELLQHMRGVQRQNPQQLQTQQQAERTLEQQGLRRRAEGPRQEAEVAAVADCSPELAAQGTTTADAGGSSRRGSCVFVAFRQRGGTGMQEQGHEGLPETGMPHRPHDQWGAYVLPLNEAQRLTAQLDPLRRALQALAACPATTGPITNNTTAAAAVKAYHIHLDRLPDLPMQPTLQGLLLGYPLVYDVRSHDEAAIASRCLAVQGLMLHRVVALPGGQLEAILYNRCSERSSTIADGRNTAQGHGVVRSGPKQQQQRRQQQGSNQVQLPERDLETLCGFSVPRSLAGEAQVAAAVREWHAQLTARLAVAGNRETGFVQSVAAPGSGLIGKGGKGREGSFCYGGSWFHRCEMEVEEHLTPNVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX23</accession>
    <name>A0ABQ5RX23_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyltransferase family 92 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="45474" checksum="F05D4A04AD9711EA" modified="2025-10-08" version="1" fragment="single">AFPEYCFVTRYLLVHLSLPGFRPSFGQSAICWSLLTITFCSLFPLCEAARSQFPISLGVYHVRLFAKAPVSGQEKVIKLNVWLKRISKDSGRHDAASAFLLPTQTDAVEGFAWADDINNLTNNKYGHYMTTVDVPTWKPLLRLQVWGSDLVVPANLHPALHAPGGNPLEPPWSHLRPFFFYFQLNTSPSVHCFKIYEESFPDTKAPFCLPTSSLDLCSRLAPDSSFTPSQPALWTVMLPFRSWGMNGSQWERYSQRVANRLQHAVTYLRSVGATGMLLYTERFSSHVLERYPGVQQMLQDGILIFVEWEHNERLQYNSDQALVYSHAMLGLSSCGTNLWVQATDVDELLYAPYGISWPKIFKCLSNSSGSDAPALFRFGRINIIASAVGADREAVLWYSAPSAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX91</accession>
    <name>A0ABQ5RX91_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">VaNZ11_004124</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The aspartyl protease (PR) mediates the proteolytic cleavages of the Gag and Gag-Pol polyproteins after assembly of the VLP.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0229">DNA integration</keyword>
    <keyword evidence="16" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="14" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-1188">Viral release from host cell</keyword>
    <keyword evidence="15" id="KW-0917">Virion maturation</keyword>
    <keyword evidence="18" id="KW-0862">Zinc</keyword>
    <keyword evidence="18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="20">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="21">
        <location>
            <begin position="452"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="114"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="164"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="682"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="206"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="706"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="728"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="758"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="781"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="GLI61686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1034" mass="111595" checksum="D6D7A729FF347675" modified="2025-10-08" version="1">MAWDGLAAIFAANNHARRSQLRQEYTKLAMKKGEALPVYLARVRELHTDLLKVGQATSEQDMTYAMLTGLPQKYEVIVSILTTQSGELTLSSATAQLLAFEARVGSMHEASTSSSAFVSAGRGSSATGSAPRRGGSHVRGGSREPLQCWNCGKPGHLERGCTAPRMAQRSGSWSSAPRGPGYGSGGLERTRMGGHGEAAGGPSLGSDRRGYGRHDHVDGAGDRGNASPRNFAFVASGSDRLKGWYLDSGASHHLTYDADQIVDVRELAESEKFEILSVNGGIIRPTAVGTLRLIPGVNMHARVTVEEVYLVPDSFANVLSVAALDKQGGSVCFEDGQVTMRKGGEKILTGQRLGDLYALSCFSIPRSASRLRYTQPFGGVAHGKSASESMDLWHRRYGHLGLGNLERLVGEGMVSGVRFGKEQVKGAGDAGVCEPCVMSRQRRGPFPSTGHKVDQPLALIHLDVCGPMPERTPTGSLYLTVLLDDYTGFSAVAFTPTKEAVKDKVMTMVNQLETYSGFRVKEVRSDQGGEFINSPLQEFFRTKGIRHGPTVGYAPEQNGAAERLNRTLLEKMRALLAESQLPQCMWAEAVSTANYLRNISPVSGRPCTPYEAFSGEHPDVSHLRVFGCTAYVHIPKEKRNKLDPVSRKGVLVGYGDAGQYRILFSDNVVSVHSDVTFDERNVGEKAQQASRAKKSVRWRNPVDSTPDSDSDSDDDDDNDEWEPAPHTQGGGSSTGAGGSGDNSGGPTGGGDASGAGPSGSSSGAGPSVGAAGASNNFESRYPVRDRKRPSEWWRSEDPRSVAGRVHVAAATKVSEPNTYAEAVSGAQASEWKRAMDDEINSQLANGTWTLETPPPGTKLIPCRWVYKVKHNPDGSVERFKARLVAKGFVQREGVDYGELFAPTSRSSSFRVLLAVAAARKMPIHQLDVTTAFLNGELKEDLWMEQPPGYESETPGLACHLHKSIYGLKQAPRCWYDKLCEKFAEIGLFPSKSDPAMFLKVDERGVVVALVHVDDTCVASDDLELVRKVKEAIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXF5</accession>
    <name>A0ABQ5RXF5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004899</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21669">
        <property type="entry name" value="SMP_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47264">
        <property type="entry name" value="OS01G0128800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47264:SF3">
        <property type="entry name" value="SYNAPTOTAGMIN-5 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="880"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="620"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="726"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1108"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1476"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1528"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1631"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="620"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="649"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="748"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="759"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="846"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1174"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1496"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1539"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1558"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1577"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1649"/>
            <end position="1658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1658" mass="170894" checksum="01D17DA01C974550" modified="2025-10-08" version="1">MVQVAGSSQAASPSVRLPLLRLSILNPAKRCRRGAPCLHFAIHQQPQSPSGLPSRSSTHLSPRSSSSFQSAHCACGSRRRAERSVVGHAGSSSGASRGGGPKQWSGSWASVTGSYRGSYDEAAGDVSDELPEGSADEVLRGRAGKLLALGGAVSLELLLTGSLVWTPLAAAVVALVVLPGTRALTAAQAAAREAETVARAEARVRAEMAARVPVSPPESAVWMGRLMGELWQPFVQPLVLRDNLDHWADKVRRAAPAGLELQLEEMSLGTEAPQLNNFQVLADPASGRVTAINCDMAFDSSSMRVVVSGSGPLGRFTATMAGINMRGRMRLLPIPDKRIILFSFREPPTIDPKMTLEGPFLGARDIPPTSLVRSLLAAILQDCLVEPVRAAISLDPAPLVGQPIDTTVHIYVESVQGIRGGGSTPAAAPAGAQVSSAPSASSSQTSSPVPHPPQSPQQRQSDEAKVAAFLESLASGPPTSPSAAAAGGSANSSGGGGATAAVVAAASNAAVAASEAVVEGAGAIASLVPGLSAVAGSAAAEGPGRGPRQRMLQVVAINTDSKLQRETTPVPYKAGAIGSAAASEGIVVPVLQMLKLNVGHKDTIYKLLVKDVGRSGLVGSVTSSSHSPATATTPSAASSLGPPSPSFPPSGSTSSASSTPTSVAAAMVTGLPTPGLLGSVRLHVAAARDGSTLFWAAGLGGEPVAVRWRPSDGPWRVTLPLESPAFGLPPPAPQPGAVASGMPARSEAAPAAPGPTAGSNTNGGVSQGAAPSNCANGKASDPGRSQTSAAKPLTPATAAHRDGTSTAKQPPGPAAGAVSSDHIDGDGGSAQSDPSRPSQHHGGAPSTSGGSSHDAGSSSTGPLASGGAPSITLLVSTDPWVYKDAARPRGTDDSPRPRSLVLQVVEARELAAHDWAGTCDPYVRISYNGRTYRTRTLYNAHTPVWQQTFILADNRSPSSLTRPARNRLSLAVYDSGVSRDDRLGSATLNLDMASDQLLQDRWIPLQGVESGRLRVRLAAVPDSPDSPAVRCIVEALQKVARGMDPLLQVVVMSARALSPRRNVQLLGLRDAYCNVVYGGARHVTPVVRQSQTPPWHYSAIFVMQHPEDVEGQEEEVKPPLPVGEGESERRRQVDTAAGVRTGKSGRDSAASGGAAGAGSGQSKVGGSQMLRTVAGNRDAAAASARGTRGSRNGGRDVNATAVEEGQGVRNGIEVGSSADPAGALVPYPPIPGALGVPELLTSSRAAAAAGGGPARPRPRPRFRRGLNRDLLRLEVKDVAPVPPDEDLGWVELPVRALLPQPGDSWQGWLPLRRGEGAELLVRISRIEPIPMDQDGVLLPMLVQQQQQVTSKAAAASSSQPQGSSVVTMTAAGGAGGDDFGLTGTRSPPQQAMASMEKVLLDSFTDQVRAANRSAGQVGQDLRVRLGQDVIPKIQDWWEGALQRTVSTLEPPSSKVSSFLRSPLLLTSSWLAGKVAISGKPPSDPQRMLPSPPPATSQLSGQVQGSQQMPGEQQVSTPGAIQDIMAARVTADDNGKVRRASSSSGNQRAEASEVEAGASMTTAGITTSSTIDAEASDTGENSNSTGQNGLANKASKNGGSRGLKVVRGTREADGWRLGQLVARAWEQLLGPPAYQAKPRADDSLQPQQKQQERQQEKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXT2</accession>
    <name>A0ABQ5RXT2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="246" mass="25245" checksum="DBF1582D27887A0E" modified="2025-10-08" version="1">MFQGLKKAFSKGHSSEVETLEASTALRDETMPVRSKLKSEVPSSDLSVTGVSTKNGFASSFSGMHSSLPGSGGQEAWGSNLLGDAAAPLRGRSGATVLSAASPLPTSGANGLSASAITGLSQSPPTANTTAWGGTSTLLAKDASRSTSEKDAPKPSRLSGLFRSGSKNARNALTEEFTAIEPMGQSLGGSGSFHEECIEDIRWSPGSRNAQKAQAQAQAGCEDEDCPPLTAQRAVVRTGSLMATPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY43</accession>
    <name>A0ABQ5RY43_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleosome assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004945</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="40004" checksum="B35A12A584204B0D" modified="2025-10-08" version="1">MALALLTRHALRAGTVCSRVVSNKPVQVPCKSVFGRPMLAQRVLGFSVAAAPKGKQPPPPPPPVDEENELDEGMDYDPMEDLTVPQRRRVLAMKDVQKKYDLVHNEYQKELAALQHKYNEKYAPIFDERRNIFTGKTAITEKYELEDDDKPDGPVPDFWLNALANHNKVGPFITERDSEVLKYLEDVRSQVLLGEERGFKLLFYFRADNPHFSNRILEKVYVLEPEDDVVPKRFTGTSISWKEGRDVTVEETKRRVKGGKGEKSKPAFVVEKVPCDSFFTIFDPPKVPTEAEATSMSEEELEEVEEALANDFDIGFAIKDQVIPRAVEWFTGEIAPISPDEYDDEYEEGEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY84</accession>
    <name>A0ABQ5RY84_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="949"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1114"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1277"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1357"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="439"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="542"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="565"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="645"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="663"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="688"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="807"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="837"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="951"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="998"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1127"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1281"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1379" mass="136591" checksum="EDC977BDF5343A64" modified="2025-10-08" version="1" fragment="single">MLEIYEVFDASNEELLSELQNLQHLLRNSPGPNHVHYKMLYDTVCQELEERGVSHKVFQQLAPTVHLQPHASLAAGLGLGAPGPFTALLPAAVVVKQPVQPVAALYSFGSFGASGAGGGGAMPAAPPPPVAAVAGGATPPASTALAYGYGSSQHHPYLTHQQGSQPATGGMAQAVNAMVAASVRYNQPPALANVPAAGAPVAPRPQLATLPPSGLLSAPVLTPAPAAASSGATSVHALLRNYDLSVPAAAPKPLPPQPPAAVLQRPPPPVLTLQPQVQSQLPTTATPQNALQIQIQAHSPGPTPTGDVMLTPSELGSARNKARSSSGDGGGGVDSKTSGAACGGGAANSSGAGSESDGLATPAEAVAGSKAFGPGGEASTASVDSNGGGEGGSAANGGGGGKHLTPWEAAMLAFREEQRLLRKARKAIRKGPPPKPGAKKPGSVSPSHASSQHNHHQHSPLGAESSCSTRPGAKPPRPSGTGSAATTTTPGAGKAAAAAPSSPHGAAALAAGASAATRGTPASTAANGAGGGSEGPKGGKSGAAASGAGNAARSQVVGNGGKASGTGPAASSSLAAAATATAATSGAHAVRTAEEASAAATAVTCPAAASAEPPPVDLDAVPEEQGLAARTQAVPAETVAVQLREPSEPESVKTDEESHEMVAAAMWEASAAAAADAVRRAGTGGAGSVSDSDPESVSSGSQQSEQEEHGPRPEQQRGRKEEHPDSGLSVADLRVQGCVLSAPAGPARPDAVAGPASVTPPPRAMSASHAPPWRQQQPSRPGQPVLVATSVKSPPSAATSVIMPRNAWGAAPSLGPAALQPQQPMPLRGPEADTVPSESDSGADSDVGSVSDFPESPGKWTRVGRGHQVLSNERLQQPRVEDDEEARQRAIAAAAAAAIQGRSAADASQFMGSANQLARAVISDGPMKVDRWSDWDLADSSDALSAWATNHDGDPVRGDHLRAPRPVGHLAPPESLVEGPAGAHALPQTDKRAVGLEGSTGGSSSGGGSRQSVRFGEGVTMPRAGSAPHSSSGRRSAAAAEPALQSSASALAVTSGGKGGDISSSDDDGNAVGDTGESGLMLRGEGQQTIPRTGVVPAGTKAATLPNVFTAPGSNAAILRRPPVAPVSSTPTQSTGRSGPPSSNGVAQSLPRGGGGGGGSSSNLAPAATAASGTAAAASASSDAAIAAAPYLQYYSSFHEILTLRYSSLPVMLDAAALASASVAAVAGAAGGGGGGSRPPSRMLLGSAAASGIDLLERYAGLLARCEAWQAKLRQLGSTMPTEDPPTVAATTPTEPPVDDVAAVGQQQEQPQQPPLPMPMPMPQTRRYVAVIDDTAHPEVRNVVVAALRLLSPSWALDPVDQVTEQAKPEASADDDAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYD9</accession>
    <name>A0ABQ5RYD9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052258">
        <property type="entry name" value="Diverse_Func_Domain-Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37612">
        <property type="entry name" value="FIBROIN HEAVY CHAIN FIB-H LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37612:SF20">
        <property type="entry name" value="PER-HEXAMER REPEAT PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="750"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="898"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="513"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1019" mass="99356" checksum="DA2A85A844FBF351" modified="2025-10-08" version="1">MGLASWPVVHADAGQRIENHAVIPACSASPSGRVSHCSEMVAVSGRPRQQTTDEPGPVRSTYTRPRAPPGADDAGMPQFWCNSIGTASHPNSGEQARRGVQFISSDRGAGVGGGNCNTDIEGSDASSGDDVFTRGRGRKPRVLELPALPSGAASASAKGGGSGSAAAAGPAGVTLAFLESVRAASRPETTLRDLYWSLILPATRANRCAYVALLGAEQRARPTHVVVCSWDERISDVVDCVTDYLAVTSSHPPDASCAGTGAGICAGAGAGAGAGAGAGAGAGAGAGAGGTSGRLALRRSVDYPTSEYSAGIGPGGSWTSWTSLGAPGLSGPATWNLSRSPSPGPSPGLSPGAAAAGNVAASPLLAVWLDLFAVNFHPPAAGPAGGTVACGGGANSGFGAAGSFGAAGRLSISTKYMPSLDDAAVERARALPSAVASIRSVILLPGAQGMGLREPAVTLAAWHALRGRGCSGGVALAVVAPGDVDVTLTQAAFEGLAVSATKTPEDSSGVTGHGIASGSSAADSAAGVNHPNRPPSPTSTFTALDRLAVRAAREGAVGAATVAAAAAAIYAVDPLPSRTVREGLTAVALASANAFLSRPVGLGASRPSVLALPTSLPPAPATSASPILLPPPTPQPPGILQTRSLSVNALELPPSSSSYSPSPLSTSRLSLRLPQRFGSGAYGVGTINSRTLLLSAEACPPPLPPPASATAAFSPSRAPSGSVAAVALSLLRASSVKSVSLNSLWCDQAGKGPDDDDGHSGGSGGGADAATAGAKDLPVSLLDGNGGGRIKDRRGLTCTAPSSNPDIAHGSWAAATATAAGAASNDSAAGGEVPSGFRRPRYLIPPLDVSVQSLPSPSALQPALSPALGDHFGGGGGGGGGGGSGRLMQLRNSTVAPWEASPPLPAMPTRTSGGTAANGGTGPCGDAAKASVHDDPPFPPCGAGTPCAAVSDATTPGAAANVRRTVANGIGGIAGGRELSIGGDVGAHAGAAAVPRFRTAITIVAGGGHGGGGTYGLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYK7</accession>
    <name>A0ABQ5RYK7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribulose bisphosphate carboxylase small subunit, chloroplastic</fullName>
            <shortName evidence="5">RuBisCO small subunit</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RBCS</name>
        <name evidence="8" type="ORF">VaNZ11_005213</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site. Although the small subunit is not catalytic it is essential for maximal activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 6">Heterohexadecamer of 8 large and 8 small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Plastid</location>
            <location evidence="5">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">The basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the RuBisCO small chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03527">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.10">
        <property type="entry name" value="Ribulose bisphosphate carboxylase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00859">
        <property type="entry name" value="RuBisCO_S_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024681">
        <property type="entry name" value="RuBisCO_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000894">
        <property type="entry name" value="RuBisCO_ssu_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036385">
        <property type="entry name" value="RuBisCO_ssu_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31262">
        <property type="entry name" value="RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31262:SF0">
        <property type="entry name" value="RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL SUBUNIT, CHLOROPLASTIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00101">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00152">
        <property type="entry name" value="RUBISCOSMALL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00961">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55239">
        <property type="entry name" value="RuBisCO, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0113">Calvin cycle</keyword>
    <keyword evidence="4 5 6" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="3 5 6" id="KW-0601">Photorespiration</keyword>
    <keyword evidence="1 5 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribulose bisphosphate carboxylase small subunit" evidence="7">
        <location>
            <begin position="56"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="185" mass="20684" checksum="8CB20CFB87A49FB6" modified="2025-10-08" version="1">MAALIAKSSVVAAVARPQRSSVRPCAALKPAVKAAPVAGPAQANKMMVWTPVNNKMFETFSYLPPLTDEQIAAQVDYIVANGWIPCLEFAEADKAYVSNESTVRFGPVSCLYYDNRYWTMWKLPMFGCRDPMQVLREIVACTKAFPDAYVRLVAFDNVKQVQIMGFLVQRPKSARDWQPVSKRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZU9</accession>
    <name>A0ABQ5RZU9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dihydrodipicolinate reductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_005937</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836:SF0">
        <property type="entry name" value="4-HYDROXY-TETRAHYDRODIPICOLINATE REDUCTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="DIHYDRODIPICOLINATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydrodipicolinate reductase N-terminal" evidence="3">
        <location>
            <begin position="31"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="351" mass="37533" checksum="B1E41BA35B8B9F9A" modified="2025-10-08" version="1">MSLALQRIGKCNRCNYSARSTVRVFASLPPVMVNSCTGKMGHAAAETLVAHGFPLVPHTFTGLSAGVAVKNIGVRGVPVQLVGEEKRQAALETIKRDYPNMLVVDYTLAHCVEDHVRFYSANGLPFVMGTAGGDRVRMREVAEAASVYAVLPSPAGEQAAALFALLISLDAPLPAHFEEYTYEALGRAGDADALDLLNPASAIAISQSLRLMGIQCDEAQVHRMRSIRQQRLGGGIHLEHEPRTSSANLRALEGRGLARACRLTAPGGSPTLLLRHYGLDRSAFAAGAVEAARFLAARVAEGTSQRVYDMVDVLRDLHAREEARRGSQTGQLQYSSNVVIAGPMVNAAVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S093</accession>
    <name>A0ABQ5S093_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="194"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="269"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="400" mass="42748" checksum="708A938F7AC56FD0" modified="2025-10-08" version="1" fragment="single">MAAVHSTRKQQQQQQQQQQQQQQQQQQQPRVLYRRPTSVGVPRQRDDWAADDGAVASPHGGPSPSARGTSAPSPTTTRVQRAVSQHVVWAKDATSRMARSIPRPVSAGSAAAGSIDGSTSSDGGSSTAAGAAVGGANNYPPRMYGGSPNLNAAAAALAERQAMVEALASLRPQCRCCRDGGAAPSSIGGNRKDVEGLSDEVRKLRAELVSSNEQNRVLRTERQRLEEELSRCRTNLIKAEEVLNTRERSLSPNTYMSRMYRSPATTSLVTKLREQLAAQQNQTESLRAQLDEVMRGTRATRVGELQAEAKAQERELGRQAEMLRVMKKRMEEAEARAAEALDRCNAAERLAKMQGPLVALQQAIAASEARFGRSENGSGSGAAASANATAAASSSSTTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0K0</accession>
    <name>A0ABQ5S0K0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sphingomyelin phosphodiesterase 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_005840</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024129">
        <property type="entry name" value="Sphingomy_SMPD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12988">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12988:SF6">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="918"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="945"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="434"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="438"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="467"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1067" mass="113014" checksum="D5E9B9F89F5CADE5" modified="2025-10-08" version="1">MAVPSAYGRDSFSELNRQLERTNQEPGSIRRTCRAVEECLANNRDNLRGFFDKPFSNLLRHIFGYGDADASWLNIVSKGHEGDARALVNLLAPDGKLFAAMAQADSDRLVEYIFPTERLPAHTQELLKEPQGYGIKLLGTWPQYRGRIRSDATGHPQVYLNLFEYFMFWTAFYVLRGSGNDTRRNDPRAQPSSGSSPVALGMQLVGNIINRRPVGSLQSHPYYHLLKTYLEYFLPRIGSMGGAGGADGAAGSRAAAAVASQNAGAAVCSGGASGPAYAFSMGTDNNAAGSVVWASYKPTVTAAAAGPESRGAILLSILVEFWLTDLAEPLPTAIADWASGAVLPGGVAGPIGGPGATSSMAGSPSPAPARVSTQPNIRTLTYQPPSEEIVEGLVALVRHVHVIEPDIRLGIGGRSSGAYVSPSSAAGSAVATIRSSSGGAGHQSGGGAGSGSGMALKLQPAQSGRPPWLPETPIKTPPLPPQRVLNPPTALLVHGSSATPAVQALSRKVYRQLRRAFSQWQPQSQSSLSPLISLWLSILAPWCPPHAHARKAVAAEHLAPSTSGSGAAATVGGDLYGGGAPAIINQHYHTYHERGALGAAKAAAAGVMEATVGRLQVASGGGTKDMPYKYTPAWRGHVLAHLPFYLLLLPQFASLSLSRFNRNPDLTVQELDRVLAVLAASGPELLQELHAAEAAFNDFLRPSGRRRRGGEYGEMAPWWLEQAHDFEAAAAAGSSAPGTVQPDVTGRLFLTDADGMAYTTCLLLRNAETQAHPELVAALRQSAAAVLPLDSILPAAAAEEQGPQASADVRHFPKPGLWPSHFSFLAHQDPKLAWPTQLWEHVYRGPNGRHDPMYRPIASNEIAYLVRMMLRLSERLNAMFGFDQPYQPGEEIDDPPETVLHRGLLSLRKRGFRFNLRFLAEIQMLAWILVGLAVAYWTLGPCLKILPWFICTALWCVFKNVCLYILGPIGAIWGLLRVAPGLLFGSQYGAAAGGGTLTPGRGGNAAVRGTTSTAVAGGCSAVDGVFTAGTSGGFGGDVYQRYDAYGGQLNTRSVGGGGGGGGYRFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1A8</accession>
    <name>A0ABQ5S1A8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="177" mass="19044" checksum="6F216A5789C1DDA5" modified="2025-10-08" version="1">MEQQENVFASLMGQARFLGGQAREWAQKNKPTTITGCLAFTAASGVASAAIAIGLIISIILVFFLLTSLLGAAFVLGITIVFWLCVSAWMFLAFSAIAASLAAWLVIGQLGLGLLRAAYNSHWLPWQVRVEFSDRRSRGFHPGGPVGVSRSGSSEASCTARSEARKVSFEIQDRHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1J6</accession>
    <name>A0ABQ5S1J6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="501"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="986"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1018"/>
            <end position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1206" mass="121933" checksum="26D02F4CB4FB3AC9" modified="2025-10-08" version="1">MRRASAVSPGIALRTDHKFPPSLSNATCRPIQAAPSHGKHSPSITRAGRVTNTLSGLAYKSTLRVAAFQVTEANTADSNEPQAAAAKPDFRRRLKIQKSSSSGRERNLQADAATETGLRRSHHVVKQPKSVDLSANSVEDSGCAPLPTTSPVPLTNKRWRRPPRTSQINPANADLADVSSTSNVDDNSSSNNSNNNNSNRSSLDGDPSPNSSSSSSSSNSISFSISLPLRTRIGRAFTHRPTLGRRRRRQAATGSETPVSLISDMQISQQPISTEDAATDAATLVAQPPTLTPLVLEVAIDGGLSPHQSAPAETSGLGGEGLSRGLTAPVTLEEPAVDTADIANKAGMLRSHVPPPVEAPAEGMETNSSELGTAAGAPAVAAAAVQEEQQRQQSSNACLMTMKVAAVSGAASAQSRAPEVPSLGVACGSVSEHAHGDVLVAGAADNGVLVSELEAGVIEADPRVEAGEVTAAAAAAVLPLNILELASRQLDEEVNPALGAQAQPEAVNAASGSETVDAAAGRDGEEPAVLSLAASSSSPGVDAADAALSPSSVTDGVRQSRFIPANVEELDDTAVAISVLAAAAAAGAAAAAAGQHPYPYAPRGATRRRTSTWELRPPARRPPRVVPFTGGGGSGGGGHWELQALIDAVREPTIIDLKGAVVTLTTAAVSSPSPPPSANGSEIQTELSAVTTTALVPFAVYDIPGWSSIAQLAEERAKMLAARAPTLAAGSGVSAPPPPADGVLLLHRDDVTLTNGTLQLSKDCRVVVTGRRVTLRKVEVVDVEAAGWVRQATALPATCLQSSALINVMSSNSSSLYGTGPWGIFGSVRNGAGGAVGPSLELDDCSVIASGRDRDLVRVDGGGGGAVTALTARYSRLRGGANAAVAAHGSTLNLDCCELEGYTASGVVALGASVRLVGSRLLGLAGSIVGLQAVAGSHLSALGCFLRGHSSNVECLGGSSVDMQGCVLSRPDCRYGLTMHAASAGAASGPHHTHSSPAIPDADGNDGRSLSLRRSVKSAGAYAASSSSPSSSPLSLSPSAAADPATAQAAGRMRLTDCELGHAIQLGGGELELERCTFVLRRTPGPRTAPPVQTIAPVQTIADPALVYGLNELTDDMKKTVALMRGGGGFGSGGSSVGGAGDTGAAFAGRVAAVASIRVVGCRVGGGNGEAEAVVMEAEQEACVYVSGSPDVVIRRCGGAGASGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1N6</accession>
    <name>A0ABQ5S1N6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="124" mass="13368" checksum="78070684ECBBB9FE" modified="2025-10-08" version="1" fragment="single">IFDYDVALLVLDKDAPATARPIHVGPAGGSLPRSAPMTVLGWGATEENSLSEDLRRGAVKQLDKATCQELFRPYGTAITARMMCTTGRTCAGDSGGPVIFKENVGLYDKNEDDSDDEFLDINDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S239</accession>
    <name>A0ABQ5S239_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_006256</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="9">
        <location>
            <begin position="1784"/>
            <end position="1926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="15"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="398"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="893"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1003"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1571"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="423"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1587"/>
            <end position="1629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1638"/>
            <end position="1647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI63336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2047" mass="213894" checksum="32CB8D554DEB7DB9" modified="2025-10-08" version="1">MGILGRFFSRCIGAATRQQHHESQPTAGVRQGDSSKETGGQQQLHAPNQDQGIWSQPSQPKRQNVNLPMRRSGGGIHPLCTTAELVNGDDPVVLLEFVGAECECAKLGLTLLERFKSSQGIYLNINFCGVLPHESFDVCRRAMHAAYRNCRAVRLLPRYANTALRSLLCCPSPAHYEALLAELLTSEPLLLAALKDICISLVEGTAAVGMGLGMWTGPVSLHPAGVISGIGAAHPIIVQHPLPDSTTRLLLPLTIYPVIVRRAVDAAASTSTIATDPQQQQNHASNNEILPELRKLQQQQQPPQPQILAAVMLRYGLSDGVRAMHEQFHRCLTALSRLPSSVTLLSADGRTVLYQNTGSVRYMGLRQGLMPIMTAGTARRPARSRGTAAAAAAAATVTATARSHKGDNNMNASAGDGDSQGDTTAGVTAGSSSTSLDGGGGRGSSCGLLGKFGLGSCKKNEDGLQKEGKKWDAAHADNGLGANGDPTGGARNNRGLNGCSGGGEGVLWELFTLCPQQWPEVVSELAARGRWKGIVRVPHLLTQHVAAEMAVSSTEAAEAEARQQSDAMHATCVGIGGNTSAADEATAVSGSATLEQLPGAVVGLYSRHRPTSYWNQVLDFEASWERLMLLHPVPASALTDLAVLPRPACQTATAPTHTTTTTAVATAIAAAALGPATVPPLETHAPVGMSTCGYNSSDSVAALHHISAPGVQFWSGLARASTSAAATTSRATVLEAASASATTVSDGAAGSSLSASQSSCLQKAFMLNKRIRSNAESFGGPTRTTVMPAVPEATAATTDEDESLLMILLQGATTTPAATSNIASSQFAMGSAPLQNIDMVYGTNFKGSFASVRTAASRLAPNQPGGLAITGGGGGQISPTSCTPIMSHVMLSEDAPNGSSCTSLRARSPVQGGRSTRGGTSGKRSSLDAMGAGAALPRIAATGSAKGLLGVAAVAAAAGSELENNDCTGTRSLELCSGHSSSQHQRSLWPSQQTSSLGADAVIVSRGGPFPDGQQQGGPAGDSVSATASRNGSYRGGAQQQNIQNILLNPLSQTTTATAADTIDMGRASSTSGMGAAGGRTTGHVLSPPLRRSIELRRNALGICRTFTGSSSASSRACGGSIGASENPLSTSTWALLAADGHCCTGRGGNHLPRRNTSFGAAILRAGGHPQQQQYQPSTPPTQQQLIDARIAMESPPSRPWPCEVDVARSCRSTTGMKALPIIPAGTMVTSNSTSRGGCARPFTSPRPFCGVKKIEAAGGDVHWNNDPEVALVEAGPQRQTPPPPLNVAQSMEGIREMQGSRMPIDKRATTNDKMLEPNMELVSSSGPDRMSSSSCASGDLLVRSNTILSTAMATVAAVLPDCEPVKSQQEPRLLAINRSGPSSCRHAPASQAQEVIPEVTLLKTSLLEMELAVSRALSAASSGATLLRHIENRPGDLYASFPRMSTSNTMPTSMLECSSLRLGNHEQCHQELIAEICTQIQGMDPAIGQEWEELQQQKEQHQHMEISKGMEVDLVADATAVHMPAMVITTCCVHQGKAAALLAAPSGSGSTSNGQVKAGAEDHVAQGDDVLQEGGFGPAPNAGPQLEKEQQQRDRVGLREEPLDQRGGSRADVEGHAASHPSDGHDESSGDNGGKGRSNNCHNNPELNDRTNNGNNGGDYVQKYDDGDSEEDSSGSSCCWHEVSITLLDGDCKAAAGVNDEPMLLLMQTDVTARVRAERRIARVLEAEHQLLESIFPRHVLELAATTAQNGDDRGGGAARYRLAQLPLPQNSASTATYHPMVTVLFADIKGFTSLCHQIPATRVMDFLNNLYSRLDTLLDVYGVYKVETIGDCYMVAGGLMIRDADGFMAVRGPGSVDELHAAKVMAFAKAMLREASRVLLPTTRRPVEMRIGLHSGPVMSGIVGSKMPRFCLFGDTVNTASRMESTCTPGSIHVSATTAALLPAEEWEPTGGVQVKGLGVMSTFRWQGDLLEHERTSLGLGVGKGSDSGILATAAAALVAHPKNGVVGVIKRTSAATLRTVRPRRASTTVVLSATASSKGIGTYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S261</accession>
    <name>A0ABQ5S261_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF444">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="356"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="572" mass="63872" checksum="54CFAE84D87B7E91" modified="2025-10-08" version="1">MLEQLAVYRRCRRQYPGTAACVLLPAFCRRKAEALLRGMRHVHRFEPGTVLFNGVDWEGMLAPWPGTSYSVDVWYEAPDEGAIGYGPALSALTLFAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDDFICVAEVNALGLTPESSDCPQVTLADGGKHPIVGRITLSLSIGPLHVVTRPYVLQGLAETAPYIMGSSTLRQNRARIYMETATLHMCKGTLSIKVPFLSFEHVTPKEGEAEAVPMVNFAVTAMAARIEPELVGHKEAVRLLRKGAHGLLLRPQVGCCATTAQISVDPEVEALLSQYEDVFKDIPGLPPMRPVDHTIPLMPGAQPTARPMYRLSPLELDEVKRQVTDLLSKGMIRPNTSPYSAPILFVGTKDGTLRMCIDYWGLNVSTVKNRYPLPRVDDLLDKLKGLAYFSSIDLQQGYNQIRNTASDIPKTAFRMPFGHFEYTVLSFGLTNAPATFQAVMDRMFRPFIDRFVVCYLDDILVYSKMRDEHLEHLRLMLDVMRREQLFVKRAKCFWAQPQVEYLGHIVLATGVKMDLWKVAVVRDWPAPQNLTELCKFLGLTNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S319</accession>
    <name>A0ABQ5S319_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">VTC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006790</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="VTC" evidence="6">
        <location>
            <begin position="42"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="108" mass="11968" checksum="3BB894038710AA96" modified="2025-10-08" version="1" fragment="single">TSSSFEPLANGAISRPPPPPSPLTPVQVLAAAPKRQLASDAMYDSSKISSVYFDNKQLDVYHERLVRVDGASLVRIRWYGVDAPAPEQGVFVERKKHRDAWTGEFSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S408</accession>
    <name>A0ABQ5S408_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007817</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="41"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="522" mass="57653" checksum="5ED496CEB432F97A" modified="2025-10-08" version="1" fragment="single">VHVAAATKVSEPNTYAEAVSGAQASEWKRAMDDEINSQLANGTWTLETPPPGTKLIPCRWVYKVKHNPDGSVERFKARLVAKGFVQREGVDYGELFAPTSRSSSFRVLLAVAAARKMPIHQLDVTTAFLNGELKEDLWMEQPPGYESETPGLACHLHKSIYGLKQAPRCWYDKLCEKFAEIGLFPSKSDPAMFLKVDERGVVVALVHVDDTCVASDDLELVRKVKEAIGQCFKIRDLGEARVFLGMDIRREKDGDIVLSQSRYIEQILERHNMLDAKDRATPLAMGTRAAPVTDDEGKLLEDPTPYRDLVGELNYLAVSTRPDLAYALSVLSRSMAKPTRAAMSAAKGVLRYLAGTREMGLRFPGGQGLQLDGYSDSDWAGDTVTRRSTTGYVFRVNGTAISWSSQLQRTVAASSVEAEYQALSSAVREALWLSKLSDDLKIGTSPSIIKVDSQGAMNISNNPMTSQRSKHIDVMHHLVRERVASGEVELRYCPTEKMVADVMTKALGEAKFKWCRASMGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4C7</accession>
    <name>A0ABQ5S4C7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="1">
        <location>
            <begin position="157"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="324" mass="34594" checksum="236BB8D0BEC1163A" modified="2025-10-08" version="1" fragment="single">PPPPPSPPPPAIPAPLVPALPPRCEVCITAKLLPPAVDIRPYRYNADTCASIQQNISTSINTALNNSNIRMVSFFKPNASLCSALEVSVCGTFFSYLDAVSFTTTAEELLSFWISEATGGNVCQPELEGYTVTVSTGDSSCLPVSGSIGCSLPFTPFPNCTCNTSQGILPFLVQPRYLPIPNVNKTTTEYCFLVSTITAQEIVPSTCGVANDNLTKIEWYANQNASSWIKGINLYPAVGNPTKLSANWGAAGTYTLKATPINWTSAQANGGMVCIEVKKPKSLDDVCLGFSGQCYASTFNSNKDCCPIYRTGLTALLPVVGRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4N1</accession>
    <name>A0ABQ5S4N1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007502</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028598">
        <property type="entry name" value="BOP1/Erb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="239" mass="26337" checksum="2D132119BFDBBE18" modified="2025-10-08" version="1" fragment="single">PETGQLAVWRPRPAAVGGGLEVRLRFPVNSLSWHSRGDYFASVAPTGNTQAVVVHQLSRRASQNPFKKNRGRVMRVAFHPAKPFFFVATQNHVRVYNLAKQALAKKLLGGGGSISCLAVHPSGDHVLVGSDDKRVAWYDMDLSTKPYKALRYHQQAPRSVSYHLSYPLFASSSDDGTVQVFHGMVYSDLMTNPLIVPVKILKGSHSVVRSEGVLDCAFHPNQPWIFTAGADAKILLYCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4N2</accession>
    <name>A0ABQ5S4N2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-acyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_007972</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="190"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="469"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI64646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="525" mass="55857" checksum="00AB0E380912105E" modified="2025-10-08" version="1">MSDFPYRLLGPCALTLHYLIVAGAIFGCGVRGLPGWYQALIAALLGSSLALVWTTHLVDPGIIPPKRDQDPLIAALERGDTDAVPYSGRYTKALNGTWVRSLTAREWAEEQQRLLQPPQPPQPQPSSPRGQEQHHLMQGQLRNVRKRVQESQELDEVVCQPAAPAPAVATAAVPAALPLSQALAASMIVHKYCITCHIWRPERAHHCSVCGFCMDHFDHHCGVMGNCIARLNHRFFAGFMVLAQVAGGLGLGGCVWRLRREGFPSSPSWSQVETYLLLLVGLLMFFHILMLGFGTGHCVLLALDLTTKDCISNDLGFLRRNPPCLPGARSPMGLLLRTYPVLLCGPVRLRPYAPGGCYPGHRVHAGSAAAASPSPLLSDGDAAATVAATAATRSPVGFDGGTIDAPGGVVTATTIVDSKSQGRRGEGSGEGATEAVAVAEGAAVLAANGLPHSHGFGAKDVFETTSWRQQQQEEPGVVSYPGAGFRREKGDQATRDRLLWTAGDRGNKDGSSSNGSTVPLMGPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4W9</accession>
    <name>A0ABQ5S4W9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-uridine aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_008026</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-amino-3-carboxypropyl]uridine in tRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62432"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:16092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:82930"/>
            <dbReference type="EC" id="2.5.1.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TDD superfamily. DTWD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.25" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005636">
        <property type="entry name" value="DTW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039262">
        <property type="entry name" value="DTWD2/TAPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21392">
        <property type="entry name" value="TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21392:SF0">
        <property type="entry name" value="TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03942">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01144">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DTW" evidence="8">
        <location>
            <begin position="342"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="188"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="551"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="572"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="596" mass="65508" checksum="BE5FC29A0C7EEC73" modified="2025-10-08" version="1">MDVCTAGSTNTKYHLSKIVFVSSVRCCCAASRPSNAGQSIPLLRSNPSLCLHQAHKTSPPAPTAYHTTGTLLTSHNRGTSVQRRYLPGARWPRFDPTCSRSNLFGTIPQSPRIRLGLRTSYTDRLFAGSQAAAMATALTTIQLATANEENRCEAAATSRNQAAVDTENPEPNFHGSLQVIVPGAAVEAANGGSRAAESSEPVQADAGGSGSHAQPDDSADQLGQTRDPDPRSKPSQKSKSKSRLAKTEDLDELDEPLVGVNDTEEELVSKLMQQLKGLSHKQQKLYGIWDGTHDGGKRAFVDQWRRRHSERRAMVEGLRDPARAPTERMSLRTSYILRWRKILFSCPNCWLLPGLCVCGRMRKFTLQRTRVVVHAHHGEWGSASNSGCILPTSLEGSEILLYGHPDHDVRLREILNDTTCTTALLWPGANSLLPEQLNALAEERTGGRITIVALDATWGNARRMQGWFPEETLTVRLPPESTLKENKLSLLRPVRRYRGDLESGRVSTVEAVATLLYELEGDEAMYRGLLDNLKIKVDACRLQKNRTLVYDTHNPEPRSTRHRWRGGGAVEVEGRKAGEGEGKADGGTTDESDGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4Z5</accession>
    <name>A0ABQ5S4Z5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine-rich repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_008372</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="437"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="569"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="788"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="502"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="609"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="668"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="724"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="798"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="818" mass="86765" checksum="E4C729A1CF99E892" modified="2025-10-08" version="1" fragment="single">MSSVEKSRTNQRGSQAYCSPSKSKAGTTTSQATCRSKVAQYGKDDGSGGGSSGTSHRRPTATAGPAAASTAVTTTASCRTAPYTHSSTPTAVRSRTIGSISWQGGQEPAAGGDGVDGCFDDSLGSDVEQSYTSVSRAAVERIRLRRILEGGIDGASAVTAVAVEDPAGPAQTEQDSTCEVAAASIIVMCQASGICDVSMRGVTRLPPSLYKFFRAEGGLVSELQVQGNRLSAAPPLELLPSLTRMDISANLLSSVPDSLGKLLKLRALDMSRNVKLIALPDSLCDLTGLTSLDLSHNALQCLPLTMGRLNRLVEMRLDSNNLSYLPPECVGLPSLTALHVSYNRLTALPSELGGASSLTGIFVNHNKLTALPATLGQLRQRLADLHLHNNELTVLPRELGLLVALTRITTGCNPLVHPPPDVARRGLAAVREYLLADWPETEQGTERPVQEGDGGGDDEVASPGQEQVPEGNGAGGSGGEPRMGVSAGRRRRLVLSAACKSCADKEDRIEELGQLLREAGERTERYERQLEQSQEKLRRQAQDLSVHAQRSVQLDAEISRLNRQLAAAASAPTLGRRPAAGGGSSGCERRTEEVEEHLRRELTAARSAAEVAGAAAEAAERDVRKLRRDLERREQEVAAHRERGEQLSASLGQERERCIAAVSAATDAAQKLEELQVAAKERDELRKKLETSKKANQDAWKAAHVAREGQLSAEATADVTQGKLVAAEAASRRLAQQLQEEKAAAGRAAAEAESRQMQLQTELTRLQADLAALMAERTVLHDLAHSKVPATGRADGSSIGTSYTAGPSPQTATAFGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4Z9</accession>
    <name>A0ABQ5S4Z9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="43"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="230" mass="25746" checksum="5DFD048D0C86CC88" modified="2025-10-08" version="1" fragment="single">VQRSFWWPRLAKDAANFVQTCSLCQRNKALSGKGHGLLQPLPVPDAPWESVSMDFVVALPKTEGGYDAVFVMVDRLTKMVHLAPCTSSCTAEQTARLFFDNVVRLHGVLKNVISDRGGQFTSKFWEALCKLVGMRVNLSTAYHPQTDGQTERTNRTLGDMLRNYAGRTPLVWDTYLTAAEFALNNAVNRSTGKSPFFLNYGFHPALPVWRELEVNVPAAKLFAKSFMSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5B0</accession>
    <name>A0ABQ5S5B0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI65026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052267">
        <property type="entry name" value="N-DRC_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14690">
        <property type="entry name" value="IQ MOTIF CONTAINING WITH AAA DOMAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14690:SF0">
        <property type="entry name" value="IQ MOTIF CONTAINING WITH AAA DOMAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="2">
        <location>
            <begin position="571"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="809"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="820"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="833" mass="94963" checksum="C93CDB0D5ADC3234" modified="2025-10-08" version="1">MANVTYDIMWREAMMELLDQLEAENPEDPTLAPKDLSEWACIYIKYLQVMRKLETSYDQMVHPQKRQDMRKALEACIGRMLEIRHWMVKLNRGLDFINLDDILVDLKLGPEVLEVPVPKYFIEDRAKELDDRDKFLEALIEKYNVKGPAASPIIRIGAPLGEDEAILMIQKNERGRQARERARLAAIAKRQRQIEDRRVRLGVTLSHDEAARKIQAAIRGWLWRRRIKKESDKELMFIGMKPKPRDVKRDPQTSEQKNLMRRKRVQLEHVREYDEAIVTLKGKVRELEGQDMRETIQDKVNAWFVEKRNPQTGEYPDFPEASDGGSRVILNPPPPTLASILEDVGDGKGKKDPKKDVAKKKDPKKDAKKDKKDDGASAAATERIGAVFIPAIEAAVQEFVAKWQDRDETGNFHQKYDQELVKDELRPIVFEEIRLQVDDEMRVLLQNLKDLVEAERAAKLGKKGKKKKKGKKKGKKKGKGKKGKKKKDPTADRSIESLFAELVSNGILQQCPHVHVRDYLGSSSFMAATLEKANIIPDPSMAQVRQALTEYCILPLGSQFIHERSPYIKSLLLYGAEKTGKTLMAQAIANLSGANFFDISPRNTDGKYPGKNVAMMVHMVFKVARTMAPSVIYIDEAEKVFLTDKKKLREFGSQEPFSRIKKELLKEVKGLSTGERVLVLGNSREPYMCVKKDERAFMGFWSKHIFMPLPDYASRRVVWPGLFERHQGRLTYEFDLSTLAHISDGYSAGQLDMVVHSLLTKRRIERLRIAPVDIPEVLQWMCKVEPISKEMDEALRRFMDKTPAMAVLKGGGKPGSAGKKGGKKKGAGGKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6Q1</accession>
    <name>A0ABQ5S6Q1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycoside hydrolase family 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_008841</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF36">
        <property type="entry name" value="LP05116P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="35552" checksum="09325923E144FFF2" modified="2025-10-08" version="1" fragment="single">NYYTRVVLDWRLRFTCRPGEVLTDMGWPVVPEGLYDAIVHSANLGMPLYVTETGIADGRDDRRAPLIAAYWQQVARAVAEGHDVRGFYYWTLCDNYEWHLGYNMKFGLFEWTEAPEHPRVNAAAAAIINAAPGVHKDDVMAASVAVEDDTDVVERSTELTPLWVTVGPNAAATATATIVCVSHPQSPSRQASRTAAAVDNVIALVTPVMAGDDSEVEQAVTDAKAKATKWSGRRLRQGSLELVRRFVATPDELSAAWHSLSETVWPHVPLPEALRRPAAPPLRTHAGAALAAMLVPLGNALAVSARGLATACRRLTRKTRRWTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S730</accession>
    <name>A0ABQ5S730_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF190">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="70811" checksum="02F3D5C31F09C9C0" modified="2025-10-08" version="1" fragment="single">HRARLRGVCRKFRTLVNNTIKKIKLDPTYSEDALQWQLYNRFPSLEHIEIMKPSEVDCFTDQTFAQLALKDLSRLPQLTSFNLENCTQLTVAGMAALALACPRISRLIIPQVCQLCMSDSMLLVLARLPELSKLRVQCCKHCDEVALAYLSDLKRLSALELEDYGQALRQVTQLTSLTSLRSVMVWSDYTVDTVLEANDQGLVGGGGHGHGHGHGHGAFLGPHAGVGIGGGGGGGGGGGGVAGGGMEHHPQGPAAGPAPGVNPIMAAAEAAAAAAAVANLNLLQHAEAAAVAAAAALGLGGVGDGGGQGGGLGLGLNGGGGGGGGAGGGGPAEAALAAALQNGHVDAAFVHQLQLTEQRLQVLNNIFEGKSALLSWVTGMTGLTHVDIQRVDISADDLQRFAALPGLLHLGVGDLNLGAGLKETEMDTLHPLTSVTKLVSHNILNEAHLRTTFPSLRALSCDSADSGLSHISELTGLVNLFLWNSPNGAISDAGVAHLSALWRLETFRLEGSTAISDGGWVDFAAAHSALTRVDLVKCPNISVKSHIKYSSAQLSSAQLPGHIPRRQCDFVASHVHVIAFVTGRRPVTGCADIGFILSIRYLPRLERVSFSDCPLIDTRTLAALLAFCTRLEHLELQDCSSVSLASLQRLLSTAARPDLEVVYKKGGAIRTLHCSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7B0</accession>
    <name>A0ABQ5S7B0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MAGE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1210">
        <property type="entry name" value="MAGE homology domain, winged helix WH2 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041899">
        <property type="entry name" value="MAGE_WH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002190">
        <property type="entry name" value="MHD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01454">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01373">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAGE" evidence="2">
        <location>
            <begin position="258"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="503" mass="53127" checksum="292FF12CCC256DA1" modified="2025-10-08" version="1">MSKRVKREPTQHRKPIDVVIESSKNSEDSSENGYSDDDFDFGNAASPPRRGSQRVVGASRGGRAGAARAAAAAAGHGSRGRGRGQGRKPASEGEDSDSVIVIDSDEDDGGCGGGGGDGGSSQAARGGRSMVQSRDGALAPAPAIEHTPMPGGHGRGRGRGRGRGRGSQLMDLDAGPGSEGEEDQQGSGGAGAGRTGGTQQQRRAYTAPTFPLEIAAEELATMRRLIKRYRDEWEAAKESCSFWGELSSADKDSLAGSVLRFMLFSARAKPGHPVPRAKLNAAIKEVLGSNRHLKGKTLGTVWLPWARYKAITSLGYDIVELVRPRGGGNAEVATAADGAAGGEGSQYYVLRTVLPTALRCEFVDGSEGSRPEDGARIVVLALIMHNGGKMQDDELHRYMSMMGFPPDRGQPHPQLGSFDDLINRMKDQRYLCLDKQGQAPTADGNTTLLLSWGEAAHSEVTTEQLKATLEPFFKGMENHVEGGEEEGDTDEEGGGGPGSSRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7M3</accession>
    <name>A0ABQ5S7M3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008691</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 5"/>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044609">
        <property type="entry name" value="FKBP2/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779:SF7">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046931430" description="peptidylprolyl isomerase" evidence="6">
        <location>
            <begin position="22"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="49"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="138" mass="15092" checksum="081FB9EF88C461E2" modified="2025-10-08" version="1">MVRRGPFLAVLLLCALSFAYAAERFKPVKKLQIGVKFRPEKCDRRTRSGDVVHVHYTGTLTDGTKFDSSVDRGTPFVFTLGEGRVIKGWDQGLLGMCIGEKRKLKIPSHLGYGDSGSPPKIPGGATLIFDVELVNVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8C3</accession>
    <name>A0ABQ5S8C3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamate--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_009290</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049940">
        <property type="entry name" value="GluQ/Sye"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311:SF2">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="10">
        <location>
            <begin position="60"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="11">
        <location>
            <begin position="408"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI65683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="562" mass="61391" checksum="5E4282E0604D7B27" modified="2025-10-08" version="1">MASTCPKQLLSRGVFMRAPCCFRALKVSSTRRGHVATFAAAAVQEAPAAAPAAAPANGHQVRVRFAPSPTGNLHVGGARTALFNWLYARKTGGKFILRIEDTDTARSTRASEEAMIRDLKWLGLDWDEGPDVGGGCGPYRQSERTDIYKAQVEKLVAAGKAYPCFCTDEDLEAMKKEAEERKLPPIYRGKWATATPEEVDQAKAKGLPYCYRFRVPPNQEIRIHDLIRGEVTWNTDTLGDFVVLRSNGMPVYNFCVAVDDALMGITHVLRAEEHLPNTLRQMLIYQALGFPVPQFGHVSLILAPDKSKLSKRHGATSVGEFRNQGYLAPAMLNFLSLLGWNDGTEQEIFTVDELTSKFGLERITKSAAVFDKVKLGWMNGQHLRCLPEEESTSMAVSHLVTCGLLASADSAFAAAAVKLVSKSMELVTDCEPEVRKLLGYPLSETLASADAKAVVDDAFAEVARAVVTAFDHGELTAALAKGHDGYKAWVNGIGKAQKRKGKRLFMPMRIALTGRMAGPDVGEQLEVLSKLASPAEVVEQGAYVPLADRIAQLKAWLQQQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8P7</accession>
    <name>A0ABQ5S8P7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_009219</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="523"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="445"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="628" mass="66733" checksum="9564045059D81F0B" modified="2025-10-08" version="1">MPDSEEKPHEQRRRQYLPLRRSLLTLSEMYVHRDIVVLFLARTVRQAAYGTVGVILALYLRAVGLTNDQLGILLTLTLLGDSVISLAVTRWADRIGRRLMLVSSCLLVILSGLVYGEVLQPSFGLLLVAATVGVLSPSGNEVGPFMALEQSILAELVSPGNRTHVFAWYNLVGYSMAAVGALAAGGALTWAQRRYGISELDGLRVVFLQYGISGGVLLGLFALLSEQVERPERRERCQVERKACVNIAVDAGTGSMAVVCDEEAGLQEPLLAAAAATEQEQQRGEEDVNDGWKWMMSLPSEAPPAGLAPEGVLTGIALEGDSGSGGDSTSPESAGMEPDGSSQAVHMTVSTRVVESFPLDDLTEYASEWHGSEFDVCDTPMHRAALSLPPLDNDSDGDGDGSDIGTSEAVHVHVGKSGDVDPNMNDGAVSGPPGLMGLSARSCSVVAQLSLLFAADSFAGGLVTGTLLSYFFQTKYGVSMAYLGSLLFGANMLAAVSSLLSGSVAARIGLINTMVFTHLPSNILMLLIPLMPSLSLATVMVFLRFSISQMDVAPRASYVAGVVRPDERTAAMGIVNISKSIGAAFGPLLTGWLAERGLFHWSFFLGGGIKIAYDLALLWRFAHVAPEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9K1</accession>
    <name>A0ABQ5S9K1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3456 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010058</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000030">
        <property type="protein sequence ID" value="GLI66296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042415">
        <property type="entry name" value="CNPY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021852">
        <property type="entry name" value="DUF3456"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13341">
        <property type="entry name" value="MIR-INTERACTING SAPOSIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13341:SF2">
        <property type="entry name" value="PROTEIN SEELE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11938">
        <property type="entry name" value="DUF3456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046226450" description="DUF3456 domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3456" evidence="3">
        <location>
            <begin position="31"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="232" mass="25405" checksum="692D44E64FCF33D8" modified="2025-10-08" version="1">MLKLLFSVFLAAVYLLKGNDAAFLKKIEAPCTACKSVAAELQRRINNEPVRNHLDLRHRLDKDGQRYGKVIAYRSSELRAVELLDGLCNIMRDYSLVSPLGKKTKFWLKVKGEGVGNTGGVTRPQREEEDEKSKRLEAYCGTLIEEYEEEVYEGIMKGGFDTQGVESVLCGKIISPCRRAAESESQSSPSASVTDDGSDSEREATPEEAAAAASGYVESATITADAEGNLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9K9</accession>
    <name>A0ABQ5S9K9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation initiation factor IF2/IF5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_009952</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="5">
        <location>
            <begin position="143"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="54"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="276" mass="30873" checksum="53ACD24DC9563D2C" modified="2025-10-08" version="1">MADDEVEQAAEEVKQLEVNDDLDLSLDLGKKKKKKKKDLVLDLDGGDAVVAPAAPAAEADDGMEMSLDLDLSKKRKKKKKERQEDANFEEGEEGAAGPADDGVEKLVGKFPWSASDREYDYDELLERVFGILREKNPELTGERRRTVLKPPQVAREGTKKTVFTNFMDLCKAMNRQHEHVSQYLLAELGTSGNLDGQQRLIVKGRFLPKSFETVLRRYVNEYVLCPGCKSVDTLLDKDSATRLMHLRCQQCGASRTVQAIKAGFVARVTKRVADAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA74</accession>
    <name>A0ABQ5SA74_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009698</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF62">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN 60E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="592"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="634"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="681"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="773"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="225"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="565"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="869"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="918"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="872"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="918"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI66096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="938" mass="101510" checksum="89522F599ABDDA12" modified="2025-10-08" version="1" fragment="single">RRSRSIVAESEGSTDSPHIHHQQLQQYILPHDSGASNGGGSGAAADDSGIPAKPCSFGHTGLPDIDVTRSRLGGDFASGQCGFAAEVTAAGTIGDCSSRGWDGGGDGDGDTKRPDADANADANTGANADAITIAIARLSPPQSPPPPRVIDISTHRHHRLQIRVDEDEEVEEVDEDMDSKSNESTRWYISGVKGEGPAPYLAYPALCCIDGRTRVRQLASAIILHSWFDYGILTAILGSSAILAVDSPRLEQSSPLGRAVAVLDVVFTAIFTLEMLIKVLAKGLILHRHAYLRNGWDILDFLIVSTSLLSLGLTVGGVGGSSTSSALKGVRLIRALRPLRLIRRLRGMQRVVETLIRSIPTLFEVLLFGVFQFGIFGILGVQLFAGKMSVCSQESVNGTWVAYRSQCVQGVTYVCSEDDMCPDPGSEAVRWWGPPLRNFDHLGSALLTLFTVITLDGYMEVARSCMDAVGVDHVPEENHAPYMGLYVLVFIFLGSFFWVNLLVSVIIDHYARIVAEEGDLLVSKQAKEYIRIFQFERNGKDVWKGGCPADASRLRRFCWKVASHPRFHNAVTANIIINILAMAMVYDNTPRAYDVALALVNVVCTCGFAVEAWFKIVALGFRKYLRDHWNKLDLFIIAVSIPDIVSTFTPMSAATGFVTVLRLLRVLRLFKLIKNARGLRTLFNTLIASSPAIANVGSLLLLIMYIYAVIGMNMYGNFGSPFDQSGSHATYNNIGAAMATQFRLFTGDGWGDLMATGMNCDANQYQCDTGASALGAAVFFCSFVLVAIFIMLNLVIAVVVDNFIDNAQMEGLLKTTNFVDVLKMVITLRVFVLLMRHKIDTIRKLHRASQLSMPASSRLIIGRRARSRNIFSSGPSTPTTPALPTAAGFRGVSATTGSPGMPALGALGTNGLGGGSGCLSPRSVSGSQRSSEFIPRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAQ9</accession>
    <name>A0ABQ5SAQ9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000256</name>
        <name evidence="2" type="ORF">VaNZ11_011059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="2 3" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000074">
        <property type="protein sequence ID" value="GLI66970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="126" mass="13945" checksum="79FBA2FFB0E7EAB0" modified="2025-10-08" version="1" fragment="single">LRLLDELDLEYKPFGDWGNGPLTAAALSALQLDPKPFVAQAISSSKLLKLRDHDLRTRFGLDQIGINKVALLQDGHKMFTGIEAMGPKSSRGSISIGNMEQYLVIKHNMREANAKTLSMESFGEMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB89</accession>
    <name>A0ABQ5SB89_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidylprolyl isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011383</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46873">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46873:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14295">
        <property type="entry name" value="PAN_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Apple" evidence="2">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="2">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="1">
        <location>
            <begin position="231"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="41157" checksum="0716C0384167DE9A" modified="2025-10-08" version="1">MVLKLGLLIVTFGCIALLSLAYLHFKSLCPHSVPTHLTDLDSLLNLLHEWERQGETEADSQSVSRVADELATAIAIARTAQTAPPPFLSSRGLYSTCNQLATEQAAIRALSLAGTQCHLAWHTEYWGDTVVWGSAHHAGSAAECCAACSSHQLAAARGGLDKGPNSTTCNVWVYCGDAERCGSHYQECWLKHLAKLPPPPQPGANSIWTSGVVYPGDEWLQPYDSMKTLMMHFTTGDVVVELLPDLAPRSVHEIRRLGAILEGGACDGCKLYRVETNFLVQGVIFHPGGYVGTTRLPNPQQKKVMERGLVCWAGCGGGPDFFVNLIDQSGFGDCHLCWGLIRDMALMDAIVKLPTKPKTNPNDMTFLTQELHFNITLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBH6</accession>
    <name>A0ABQ5SBH6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000034">
        <property type="protein sequence ID" value="GLI66546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="154" mass="16392" checksum="1FEA2A9DD7B89602" modified="2025-10-08" version="1" fragment="single">QVSDLISQLEKLRSGAAMMEARKGGLSGASTDLQGSSITAKFAIIPAASCPAMGATSPPSAVEVADAGAASIFINKAAQQPQELYQPLELTQPPKPHPKTPERRLPPEARNWGLQDCRSWAGEQHWSSWAEVQAFTKTLEPTLAEAIREASKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCA5</accession>
    <name>A0ABQ5SCA5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="616"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1226"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="370"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="467"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1226"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1266" mass="118622" checksum="BDF962038EDE8BDB" modified="2025-10-08" version="1" fragment="single">MLRAVRGWVSVFTEQKDAPGPGPSIMEPERQVEAQMRQGHQPIRHEAFREIVAETAQRAQLLLNEPARDTRTFLSPVRLPGPGFSGPGVTDAGPAYLVSARKVAKRPASEMEQPTPFANFQHVTPMAAPRGPGLDASVYRSSKRHASTGPHTGPWWLKPGANTRQSGAPPAPGFQPFSEARRLATTPFSSRLGMGTGGKHTAGTTVFTSTTGVGEAIGAAGDGTVDATSEAARAQWQRAVRVSVSTVRPAQGRPSLQPRPTSIKRPRRRWYETGIDEDSSDDDVLEKVPATASDNVQTRNPAPATPIGLFGKAAAAALALATPPPGPTASTVATSTRGISGFGAAAGADGGAAAGKSTVVASDTSRPGEGPATAGTNAQPATAAASAGEDVSTVSSGGGGTGSATRTAAQIPAAGAAPVGTSTAPSLFGGSSAPSAGVASTAAALPGARFPTSMLAATPLVPTSTTAGEPSSSSVPAVASGAAAPSAPTGGAATATKSSAGGFTFGSNRGEDLSTTTTSAGTAVQSLAAPGAGEAAAKPLASIAAPKRVDSSSTGIRTEDAVAKPASSEFSFGAAAPANASAPKPFAGFGVPVPSSQAVEGAAQSYTAAISGKPADAAAPSAGAASQQPSGFSLGANTTAADTSAAPSVTAATTQPVNPFAAVSSSAAPTSSFSFGSSSAPFPAAAPGPAAAVAPGTSAAPATPTSPLLGTSTASAVGSSSAGVKFSFGGAAPSAPTEDAAPSAFGGSSAPILGPPTSAAGAPAAASAGPTFGSAFGACTAASTAASSSAPAMSTATGFGGAAAPALGAAAAPTSNAAPGAAGSFTFVGSAALPSRATVSSGGTVPSFGAPSGAPTFGVPPAGSLSIPSAAASAPALSGFGASASASLLPSSSAPAFGATANAPSFGTTSTSFGGAGANTGLFPTAAAASAPAFGTATSALTFGGAASAATFGAAASAPSFGSTTTASAAAGPSAFSFGAAASQSAPSIVFGGSAGSAQAHQPSSVTAPATPGFGFGTSSGGGAVPTFGGASATTTSAFGAATSGPSFGSSGGGAPATFGSTAPTAFRAGSGPSFSFGPAPATASFGAASAPAFGTSSTPTFGASSAPAFGGASAPAFGASSAPAFGASSAPAFGASSAPAFGASPAPTFGASSAPAFGNASPAPSFGTGSTHAFGSGTPTFGACSAPTFGASTAAPAFGAPATASPFGGATTTFGAASAPSFGATTAPAFGAAPQPAPAGPTFGVPQPGGQPFGSAPAPVFGAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCC3</accession>
    <name>A0ABQ5SCC3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011770</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="545"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="641"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="572"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="905" mass="97735" checksum="08753FDE3E57BEEB" modified="2025-10-08" version="1">MGCLLSTPSPPPSTGLPTGAVQSPGHQASDAQAVRQLVAASKANDEHGSTPNSNGGVVAADGGGCSHPAGPASSRGDSGKDSTRDGTATPVQDKAAASTPGRKVNTNKHTLSAVDKGTQLIESLSVIEGTAVEKVEAFTSLLVGESYVQFASISVVSEDEEVFMVLTASGVGSDFLPKHYLLNVNSSHCSVQRVVSEGTACFWAAGEHNANELPEDWKCLFKMQKLKSFLAVPIKVGGQLVGVLNVALVIEVDEQKHWWGLQLRLLAAVLAQHFHDAHLRSKVSWLQSIAKQDTVEQLSMATLKGLTGIFDALQDLSFYIALGLPGRPCAVMFSLSGEMVQTAIGERKSPDFQEHVAMHGFMTSETLLENALATGQVMKVANMRRYLRLYKTGADVAILYKSEGRSPASVMVLPLVAPDAARIGGLYAISRTATDFGMVRRELMDLVKLMVPTLQQKLYSELSKKLIEVSESCKFECLSVTGTTAKDELGTSDSSTLESTKDLVLGTRPEKDALIAELQEQIRRQLAKVYKGHPDPGPNNMLEDLEVYDELGRGGYGTVYRAIYKGQSAAVKVVCDRDVDNGSLNGALELALLTSISHPNIIQIHTYYADVPLNSIKFVRGHNNPASQREADVAVGPSAGAGGGAAGGSGKEVGSGRLSRPGEQNCQVVLVMDFCDMGSLADVLNTSLLRHHAGKVAMPAILHCLLEVASALHYLHSIGIVHYDLKPENVLLKSSTIDSRGFNCKVADFGLSNIVSDNSYFNKHAEGTITHLAPEAMQPNGKLTRAVDVYAFGIMMWELYTGLRPYTGYRTIDVAVKVSKEGLRPEFPSATHPSFKELAQRCWQGDRRLRPTFRQVMDRIHEMLKDHELMGRESLLTLSSSAARLPVYPDYLQDAADNEFPLHIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCS6</accession>
    <name>A0ABQ5SCS6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycoprotease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">GCP1</name>
        <name evidence="10" type="ORF">VaNZ11_011978</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="6">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd24134">
        <property type="entry name" value="ASKHA_NBD_OSGEPL1_QRI7_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003442">
        <property type="entry name" value="T6A_TsaE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02367">
        <property type="entry name" value="TsaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="HIT" evidence="9">
        <location>
            <begin position="35"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="365"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="793"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="879"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="405"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="892"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1015" mass="106228" checksum="34334E59E75AE1A2" modified="2025-10-08" version="1">MEAIEPPTKRRKMAQTGVSAAVPPVTSYRYNEKNVFSLLLQSDGLEIPLVYDSQYTFAALDKYPIVRGHALLIVKYPAATLLDSLPPMVAQATMEDLQALVRAVQCATGCAGIRVMQASGPAAGQSVPQLHFHVIPVYLHESSSTALVTSIPPPQRSQLLAADAGPLLAHIRNMLPKSYAPGYHVWASNESDMEALGWALQQSCSIPGTVLALSGHLGAGKSTISRGFVRAFVRDSLLEVPSPTFLLCLSYQDDTAYEDKVVDGGGGGEGVGRSAADDEVVPGKGAAPPKVAVALPERARCVHHMDPYRLGQKCDKMAGLIDFETAFRREVCLIEWPDKMPGSVMAMAERGLAVDVSGMGAQASGRLITMTPLGDRFKPRPDKGRKGGKGGGRDNSKPAATDPKQQPQPQEQQQQPELATQPAETPGKSTGEPTAQILEGAGVDDDFGYAQLLLHWRQQYPAAIPPAEVSWRGAVEAAEANPGDAGADALAGHCSGTVATAAARNINAAAAAALETGDPSQWLVLGIESSCDDTAAAVVRGDGTVLSHQIASQTGLHEQYGGVKPDVARQAHASAIQSTVDSALQQAGVEPGALTAVAVTSGPGLALCLQVGVQYGLSLAAQHGLPYIPVHHMEAHAMMTRLPSVNTTPMLFPSLLLLVSGGHNMLVLCEGVGRHRIIGTTLDDSVGECFDKIARVAGITAVPGGPHLERLAAAARPDAHVRYPLTMPLSNGAQKDSCNFSFSGLKTQVMTLISQKRKELGLPDLPLPPALAAVAGTAGGSAGAGAGGAGDVAMDTGKGPLEAGAKGGDVEGPAEDGLVDEAAGSALRAEAAARQAASEAEFSAAVADISASFQRTAVRFLQQRTRRALEWLREDAAQQLGSATTPAESGDVGERKDLGTGSRQERRQQPGFVRSLVVAGGVAANMAVRAGLSDVAEEFGLPCVYPPVRYCTDNGLMVAWTGVERLKMGLYQQPPRRGADGTIDAHVQVIPRWPIGPMDPRSTANKGRAHIKILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD10</accession>
    <name>A0ABQ5SD10_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_011761</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00003">
        <property type="entry name" value="copper ion binding protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594:SF4">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00942">
        <property type="entry name" value="CUATPASEI"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="607"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="646"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1079"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1107"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="14">
        <location>
            <begin position="54"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="14">
        <location>
            <begin position="132"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="14">
        <location>
            <begin position="206"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="806"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1142"/>
            <end position="1202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI67539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1202" mass="125111" checksum="9D26C2A985702023" modified="2025-10-08" version="1">MMQRLNQFLSALRYEKLPEKEKDDREAQPCPSPKPQPASTGHRRQHAEEKGHWPVALLSVKGMTCAACSRAVEAALSSVTGVKRVSVALLQESAEVHYDESSVSPEELVSAVEDAGFEGGLVNVRHPRQQLEVLRMRVTGMICASCSTAVEKALLSQAGVSKAAVALASGEVEAQFDSASVTAEALMTAVEDAGFEASLVSQGGLETLSLRVYGMTSTASATAVEAALRRVAGVARAGVRLEEGLAEVWYDPNVTGPRDCIAAIERCDGGGGGSSTTGTSPAQPLTAELLRSLSPWGAGSSGGGAGGGSKGSGGTMSAADREVRYWWGLFSTSLFFTVPVFIVAMVLPMIPGSEGFMSTPIIWKIPCNQVVKWALATPVQFIVGWRFHRGAFKALRRGTANMDVLVSLGTNAAYLYSVISILFHRYEGHRLEMDEPMKVDPNGYVPTDFFETSAMLITFILLGKYLEAAAKGRTSAALAALAALVPDTATLVVLDEATGAVLDCREVPSALIHRGDMLRVLPGSKVPTDGVIVEGQSYLNEAMVTGESAPKWKRPGDVVVGGTINTSNPLLVRATRVGSETVLSQIVRLVEHAQMTKAPVQAFADRVAAIFVPLVIVMALLTWLGWYVAGLTGVLKDALPPGHTPFLFALLFGIAVLVIACPCALGLATPTAVMVGTGVAAQMGILIKGADALEAASKVTVVVFDKTGTLTRGKPTVTDWRLFGAVEGGSGAVGSPWPGLGVPLDLATLCRCVGAAESASEHPLARAILDFCRLHLVMAQHGATTRDTAAAAAAAAVAGALGGYPSKDLHEESGSGRSGPRASHREQAAELEVLLPKNLDALVTPGVGITCAVSAEHLAQLAAAAAGAAAASSRSGSRGGSAAALPAASGSAAASSTVRVAVGNRLLMQQELVDVPAEAEAFNEPMESCGHTVVHVALNGRLAGVLAVADPLKAEAAGVVAALRGRGIQVVMLTGDNWRTARALAGQLGVDMVHAETLPKTKALKIRELQSSGKVVAMVGDGINDSPALAAADVGMAIGSGTDIAIEAADYVLMRDDLEDVLVALDISKRTFNRIRLNYLWAMGYNTLMIPVAAGVFYPITHHQLPPWLAGLCMAFSSVSVVASSLLLRRYRRPPKVLREVVSGPSRGAARGLSPHRTSLHEEGDGGASGSGRSPLLARSGPTAANGGGSKRASLELMRSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDX0</accession>
    <name>A0ABQ5SDX0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="407" mass="44399" checksum="D47CE00A2AB77289" modified="2025-10-08" version="1">MERDVPCVPHMFVQRVDALPEEILEGVLGALEPRLEERRAPHWFQNFGLGTDAKPPGGHESRSKADLRNLLQRHRRKELAADADSADFSSEPYDSIPRQMQLQWRVYARLYPDEVAESEKLACMIKSLRPDSWPTDDKQQSSGADGGASTLFGALPSVTSLQRTLRNLMHRAQGPAEAVSTAASATDADVSVMVATAPRRAPAVAASVGFASSSSSSCRPAFGSSLTNTCACRELWQRHDPILHKLNQSLDYWYELHREQCRPLPQGRKARLLVVRGVDLPAEWKGSIRPGASAAVGHAKVGGGWPGGLIRCCAAVARAGAGALGLRRRPRLWDVVGAAGLETKEMHSRQQQEDARQQEQAGSLKSRPRWGASVKSTGDWGLVAAQAAAGRMNFALRKTTLIPPDWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE40</accession>
    <name>A0ABQ5SE40_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="573" mass="59989" checksum="5D08392F727D2638" modified="2025-10-08" version="1">MLMRKASVSGYAIWPRQVITRARSAFGPRRRLREPKLMRVSKTTNIQTSSTVKPPATSSAAAGPPEAPIIYRSIASLSSVESLSSFDELNDGTENFRFIPSQLEVFRNLVNSMQNCSRGLAALAFGSVVMNAFEAVEHAHHHHGEHVQSAVQLAGQAAGAAVSSVAEQAAAVADKVAVALPGVSEQASQLTAGGMDFLQEALHAVSLTDVAFCIDQIIPALLIVYAAAPFEQLARNPDEPHMELALRGVGRLSVTLQQLAWTSGSVAVVLLLTAAAEYPPIVGVASWSCLALAVARGASLWWVISQHTTSGEEVARTLATLRGGVGGRDMNPLDRAASWLALGVLLQEEERLHANHLRHQTQHNTLSHQHQQDLSHDKTHAARDVTVIGEVGSNSSDVASSGAGAGAAAAAAVYPPQPHYSFDLEEERVLLSLVEATNAAGMAISLLALATLALGLAEAGGESSNTWVSSIILAVQKALNATALFASAAAFDRALHNDDGNDDTDHLLDGLGREHSGMTGLFTSMAVLSLALILAGSVALLIPSLEDSPLGVLVGDGVMHASADLLIGVLEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE51</accession>
    <name>A0ABQ5SE51_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042856">
        <property type="entry name" value="RSP14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15599:SF1">
        <property type="entry name" value="RADIAL SPOKE HEAD 14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15599">
        <property type="entry name" value="RTDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="109"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="266"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="361" mass="37070" checksum="6D59AD3824062CE1" modified="2025-10-08" version="1">MPGPGNDLTVEERLERARLLPRVNQEVLTMCKTVDQLLHAQHISNLAQAIVCVQELAREHYAVVLALKWVVLLVDMLESDAPDVRLASCNAIAAVSMVQEGRDAVRSSGGLRPLVMLLAEGSRSSVAAAAAAALMNCSACDTCKEAIADCRGVPMLLGLIREAVARCNPVNGGAYGSGDAARSGGSAPQDLHTPGGCKAAVYAAGALMNMGGVITVQELLMAAGAVQVLEAVIRVAGPGDVVATRANFMLSWLAVASSAAAGTLDRRSEKLDSVISPSATPRSLPSVVSTRPSGQQSLRQGPSINSQQQQPLPQPQASVTTPSLGQPQSSSQHRMTSQSIAKPGSSGGPGAGKPAVVAGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEC1</accession>
    <name>A0ABQ5SEC1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="577" mass="59888" checksum="2FB4CAEB872449C3" modified="2025-10-08" version="1" fragment="single">MKLKEWFCACFQPVEVREFSSLQHTQFQRGGIDAPNPFRNAQNRTSFEYLASTERMVLCQALIDSSPEPVSVYEFATGRLLLSNAAFKNLPGGCVNLPGLFALDPASLHDALSRVHSGLCWRGLIQLPAGLQSHASLQSPWVPVVSSTRPSTGNGIVPPCPSMQRVPEMTDPAPIHNLGHASSSTRLRQALQVVAYPSLRLHGSPASTAAAAATLAYDDPGAALQQPVLLPTDSFTQWRAGGGNGKMVGLTVTAITADAATAAADTIVKATRAAALAKAAAQAAAAEAAAEAERTRPISAPTRDSPTAPPLPMLPQPAPPQVCLSSPDTTQPGATASAVANLKSTLSSGNSAATAAGGDDLTWDLALGSADPPSWSGARAVADILPDDRLPMALIRPGSTSALMGAGLAAELLGVFHQQEQQQQQKQQGNEAPTALHNGPQGFDGLELAAATVVLGAETSGAAARAAATDRVRLHHSLGEWHDGMEGRRLTVPLMARRGTEGEGGGGDITNSTATVMSESSKVPKATEAAASPQPLQPGLPARRRNNNVQPAAQPALLPHSQNLLEALTTSTWGTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEI0</accession>
    <name>A0ABQ5SEI0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tripeptidyl-peptidase 2</fullName>
            <ecNumber evidence="3">3.4.14.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Tripeptidyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_012720</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal tripeptide from a polypeptide.</text>
            <dbReference type="EC" id="3.4.14.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.10" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04857">
        <property type="entry name" value="Peptidases_S8_Tripeptidyl_Aminopeptidase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034051">
        <property type="entry name" value="TPP_II_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022232">
        <property type="entry name" value="TPPII_C_art"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046939">
        <property type="entry name" value="TPPII_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048384">
        <property type="entry name" value="TPPII_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048383">
        <property type="entry name" value="TPPII_Ig-like-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022229">
        <property type="entry name" value="TPPII_Ig-like-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046940">
        <property type="entry name" value="TPPII_Ig-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF14">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12580">
        <property type="entry name" value="TPPII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12583">
        <property type="entry name" value="TPPII_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21316">
        <property type="entry name" value="TPPII_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21223">
        <property type="entry name" value="TPPII_Ig-like-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="90"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Tripeptidyl-peptidase II first Ig-like" evidence="15">
        <location>
            <begin position="661"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Tripeptidyl-peptidase II galactose-binding" evidence="16">
        <location>
            <begin position="805"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="domain" description="Tripeptidyl peptidase II second Ig-like" evidence="13">
        <location>
            <begin position="943"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="domain" description="Tripeptidyl peptidase II C-terminal" evidence="14">
        <location>
            <begin position="1195"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="589"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1168"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1333"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="603"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1173"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1184"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1349"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI68325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1486" mass="157520" checksum="57A27AAE7C03BD6E" modified="2025-10-08" version="1">MHCLRIGPQSIKSWAARARAGAPAVSRAFRVYHRRLEVIASARARTSSVDEKPEDISMLDGDVAALFSHAMPKVEIGALKFLQSYPEYDGRGVKICIFDTGVDPGAAGLQTTSNGQPKILDVIDCTGSGDVDMSRIEKADAHGYVTGASGRKLKLHPAWSNPSGEWRVGCKHVYDLISRTLVTRLKEERKKKWEESHRRAVAEAVAALAKFDRDTGASKISSDVAGKQRTELEGRLNLLKDLAKQYEDPGPLLDCVVWYDGSNWRAALDTSQLHEPGSGRGALADFKPLTNYALERQYGTFSELDSCNFVLNMLDEGRTLSIVVDCGAHGTHVAGITAAHFPDDPGSNGIAPGAQIISCKIGDTRLGSMETGTGVVRGLIAARQHGAHLINMSYGEPTSTPDLGRFISLATELVRNHNVIFVASAGNAGPALSTVGAPGGTSSALFGVGAFVSPQLAAAGHSVLEAPPQGLQYNWSSRGPTPDGHIGVAFSAPGGAIAPVPQWTQQRRQLMNGTSMSSPNACGGIALLLSGLLARGAVIAPHRIRRALENTALPLGGDAPDAVLTYGRGLIQIGAAWDYLVTTTTTTTTTTGDSVAAAGGGRAVANTAAPAAQPTERRELPPAPASSPSPSLAPSSSTPPGPCPGPWYDIEAACVEGRGPKGRGIYLREPHESAKVQSYRITVTPKLRQGAANSSRLDVEDRLALEATVPWVTCPPALMVHAGGRSFEVIVDPSSLPPGLHYGEVVALEVDSRERGPLFRLPITVIKPLQVPLAATSINGINGNTVAGNGNSSAVGTVSLGPLSFAPGTEYRRFVAVPPGATWAELTLRAAPYDTPKLFLIRGTQLRPESSYRQHELRTQVTLSGGSEYLNSLRVCGGSTLELTLAQFWSSPGFSQLQEVELSFYGVELSSDGSSGQRPGTDLALDGAELAKKVLVSAPTWSRATRIKPEAKLTSLHIPLRPVEATLEPLTAERDALTDGRVVYRLLLTYKTTLSEPGKFKPSLPLINKQIYDSPLESQLMLVHDGATKQLLSVQDADPESVSLKKGEVLLRLVLRHDSQELLDKLKTMPLVLKRSLEGGIAVPVYGSRRDAILAAGGSGAPAGEMLLRAGDVAPLWLGPVPEDKLPKDATPGRLLAGRLTLGQLKRGGGAAPHKFAISYLVPPTPSTAAAPGSGGGGGNGGGGKDDAGADKPPGEKLNEAIRDAKIKLMKELKLSDPEQAQMFDRLFVELKEAHPNHLPLLLEHLRKMDGREAAVRRTREGVAAVCLSAQAVVAAVDKGALAAFLGVKAGEDTPEYRKAKKEMDERKAALVEALSRHVTALLDEEELRAAAATAAAAKEKEEKEENPETATTDVNETAADAPDADGDAGAKTAAAAESPNPPALANDELVGELMQELRKWADTSSESAHLLLHAKVEAQGRRYAAAIKALDKLLASDDKAVSTAAGREDISNLKHKLYLALGWGHWATLEKELQAVRFPRELTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEM7</accession>
    <name>A0ABQ5SEM7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012694</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="255" mass="28149" checksum="42D857CD0A951EB8" modified="2025-10-08" version="1">MSRVGEVAAQRPLAFAFDSPPLVTPQPVFLQPHYAVRLCIDEPMIGIPDTGRLAALCFAADTLIERVHKVPKSAAQFDVSILQEIEALCHQVAPKKHAQTTVQDLGLLELFPYLAMGLSGDKLDTVQNNPGAAYFQYISLLNQVVMMGTQLYHDACMPQHHKYAAHQIALLYQCLNMLQGDTKPIRRVVEAQFDEIKAITESRDPYLPVELSAWIQEVTFLCREEIQSCPAYVHKRLDAVLRAARGEGGAAPGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFF5</accession>
    <name>A0ABQ5SFF5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012253</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="127"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="232" mass="24820" checksum="D2DE07368DABCF86" modified="2025-10-08" version="1" fragment="single">PPLRPSPPPPPPPPPPPLPPLPLAPRTLSDYYFTPPYKYTSPMAPAGENPPPLYEYESPPPPDTNPSESIRPPGYDNKLQPSQPEDSPPLLFEYESPSPPSGPAPPPPPPPPPPPPPPPPPTHDGAVRLTGGLTAREGRVDIFLRGKWGVVCGRWTATEARVVCKQLGFNVTSVQIVRWARPPTMPTWLDGVNCARLNFNSPYKGLAACSSYGIGMIRCSAGSDVAGVRCVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFH8</accession>
    <name>A0ABQ5SFH8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">methenyltetrahydrofolate cyclohydrolase</fullName>
            <ecNumber evidence="1">3.5.4.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013171</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.9" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010783">
        <property type="entry name" value="PRK14186.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF27">
        <property type="entry name" value="BIFUNCTIONAL PROTEIN FOLD 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="2" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="6">
        <location>
            <begin position="43"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="7">
        <location>
            <begin position="179"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="385" mass="40291" checksum="6683D03FA9063C98" modified="2025-10-08" version="1">MSSESAIGNSAAVVSAAPTSVPSPGSSTALPPAASLFMKGNVIDGKAVAAKIREETAAKVAELKAKYSRVPGLAVVIVGERKDSQTYVRMKRKACEEVGIASFGADLPETATQEEVLEVVRGFNSNPDVHGILVQLPLPKHLDEQAILGAISVEKDVDGFHPLNIGRLAMKGREPLFAPCTPVGCMELLERCNIPMKGKRAVVVGRSNIVGMPAALLLNKRDATVTICHSATKDMESIVRQADIIIAAAGQAQLVKGSWVKPGAAIIDVGTNPVDDPTKTAGYRLVGDADFEECKAVAGAITPVPGGVGPMTIAILLRNTQVSGERFITGKLQCPVAEDTTAGSRKGPLSRLLKDKEQLGPWSLVAAGSLLAAGALQRVITSRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFQ9</accession>
    <name>A0ABQ5SFQ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013268</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF9">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 7" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="262"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="481"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="481"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="512"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="706" mass="75088" checksum="5145FEBF1F94FD0A" modified="2025-10-08" version="1">MVATIPCVGSRYLQVRGSIPRRKMQLQWTSAHNLNIRVKSCAFTSIPRAVPSTTGSETADAAVIDGAWKVRGERGVSFITGEAFYRAESCAGRDLAVLAAVLHRRRTGSLRVLDVMGGSGMRGARYLAQADADEVWVNEGNMDLHYAVVYNMCSAAGLPVPRVQAGCATAAITGPAGVASDSTAGLGPANQAATATEREELSGQSLRAIAAYWEGQAQRLRLEGLARDVWQWDVPRPTGSCDDALRPRQLLTPAVAEAAADSKSTACSLRDGNSGDSGSSSYSSLPRASGSGTSGLSQAQSAEPTRGHRRIRISHADANRLLTNCYMRESYYDVVDVDSFGSGTMHFPAAIDAVRYGGLIYLTGTDGFTTSGKRPARSLAAYGSYARATPWANEQGLRMIIGSAVREAAARGVTLAPIFSLYSYHGPVFRVMLRATRSAEWPANHYGFMGHCFVHGDNYTVGWRQLGKATCRCAARGRRPIQSSSNTVSGIEAANNTGVTSNGTDLGGNDAPSSACISASSPGGIHSHTRRGEHGSGSSKAGSPGNRERTEDTCEPGAEDGSPTGIQLVLSGPLWTGPLHDAAELQAMAAEAASRGWTGFGLDAAATPHLQNRVKSSRNVRPLEELLEVLLEEADPRLPPGFFSIDESVARRLVRPPSRDGLIAALRQEGFAAARCHLEARAFRTNACMVDVLRVAEERLGIPRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFU6</accession>
    <name>A0ABQ5SFU6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="381" mass="42503" checksum="575E7612687F25E4" modified="2025-10-08" version="1" fragment="single">IDRTVFNLKHKNLRRTHRLRHDSCALHRTCLLQSVRDTLPTNRSLASSAESTGHDTALLAEVLEQVKSMNNKLNKISKQVQRLETRVCELYEFTSLPLISTHFNSDFAENVDLRSAAHVVDHILGLALNLDLPSLKQRGALIGILEDHVLKDRQGLRAAVKCLIDEACEEAGVCMVALPSSFQTAAWDDIRACLQELQLGYSFPGSKGDERINDLMRCVDALETGAPPRPLPGFMEMATAMASGSCLSRITLDCRGRIDVRDTYIEFHIGAFKGVNAGYPETCQLLKRAAALFVWAYKVLEPHIYAVSVTPNGTVGTSRRERDVEAIGYTFSVGKVPPQDIMLKLYDDFEVEVNGRRSQADIAYRYFSVKPTGLHEHYAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGS1</accession>
    <name>A0ABQ5SGS1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Pyruvate kinase</fullName>
            <ecNumber evidence="4 14">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_013069</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="4 14"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="15">
        <location>
            <begin position="2"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI68599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="168" mass="18106" checksum="018AA9A40353613F" modified="2025-10-08" version="1" fragment="single">VALVAKVETRQALFNFRGILDASDGIIVSRGLLGLDVVPEKVALIQKALCSHANLAGKPVVITRVLDSMIDNPRPTRAEATDVANAVLDGADALFLGAETLRGKNPVETVRTLVSIARQAEKVFDFRHHFEWLMQAAIDAQELRDARAAGFGAPGLEDDDAATDIYML</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI80</accession>
    <name>A0ABQ5SI80_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_013578</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17973">
        <property type="entry name" value="DEXHc_DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044756">
        <property type="entry name" value="DHX15_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF109">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="64"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="253"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI69037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="710" mass="80073" checksum="BB7075035FA47379" modified="2025-10-08" version="1">MSGDRKRKLDYSEEPRRPGPGPSADGVAEGNVNPFTGRSYSQRYYDILAKRHGLPVWQAKEEFVNMIHGHQTTVLVGETGSGKTTQIPQFIAEAGYTANRKMVACTQPRRVAAMSVARRVAEEMDVVLGEEVGYSIRFEECSGPKTCVKFLTDGMLLREAMTDPLLERYSVIILDEAHERTLATDVLFGLLKEILKQRTDLKLVVMSATLEAEKFQGYFLDAPLMKVPGRLHPVEIFYTQEPERDYLEAAIRTVVQIHVCEPPGDVLLFLTGEEEIEDACRKVTKELSNMGDKVGPVKVLPLYSTLPPQQQQRIFEPAPPPAREGGPAGRKIVISTNIAETSLTIDGIVYVIDPGFAKQKVYNPRVRVESLLVSPISRASAHQRAGRAGRTQPGKCFRLYTESSFKKDLQEQTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLGAMDDDGNLTPVGSIMSEFPLDPQLAKMLVASPEFRCSNEILSIAAMLSSPNVFLRPREAAKAADEAKARFTHIDGDHLTMLNVYHAWKSHNEDSNWCYEHFLNFRSLKSADSVRTQLVRICTRMNLRLMSTPFEDKNYYSNIRKAVTAGYFMQVAHLARQGQYLTVKDNQVVHLHPSTCLDHKPDWVLYQEFVLTSKNYIRTCLDIKGEWLVDVAPHYFDLTNFPGGDCKSALQRLYAKKEKDKSDRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIA7</accession>
    <name>A0ABQ5SIA7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase sigma-70 region 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013781</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050239">
        <property type="entry name" value="Sigma-70_RNA_pol_init_factors"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR RPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603:SF47">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR SIGD, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="Sigma2 domain of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase sigma-70 region 2" evidence="2">
        <location>
            <begin position="91"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="366" mass="39219" checksum="A41E8996F178B77A" modified="2025-10-08" version="1">MQLSGLRRVLTTLLRRPPSMVEMAKSLHCDEAALRVRLDCGNRARTLLAQKNYRLVVNIAKRLVRPASAAGNGLSGSSGSGGISAAVGDGGVSLDDAITVGMEALMYAIRKFKPEAGYRLSTYATWWIRLHVSRLVQAQTGVISLPVYQRANLDRLRAVRALLRSKLPPGVVPSIQQLAEAAGMYPSQVAQLEAIERAFTGGTRSFEAPLADGEGERGFTVEDLVAQNTDAELPLASTSSLFLDSEDFAASASLSSTVEGLLASLDRRESELLRKQYGFDDEMEGESGGEDGSEVAATEFGKAGPAPGMQRLKLGRKRRTSVAQALEKLRLLAGEDSPLAEWLQLESAKASYATRSAAGYSKKSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIV5</accession>
    <name>A0ABQ5SIV5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964:SF113">
        <property type="entry name" value="USPA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="3"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="157" mass="17559" checksum="F44093DC85383438" modified="2025-10-08" version="1">MPRVLLFPVDDTDDSQKSWDWMIKNIYRDGDEVHLLNVISRLSFAATLGVPAVDFTPQINREAYEGAVRKAEAFIVKRFLARLPEHIQTTPIVHIIKSEVDTNNVGHVICVKAEELKAACVLMGSHNKGPVAEFFLGSVSQYVSHHCKVPVVIVKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJF5</accession>
    <name>A0ABQ5SJF5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_014465</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF169">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1162"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1212"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="domain" description="SBP-type" evidence="7">
        <location>
            <begin position="102"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="33"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="174"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="442"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1252" mass="131015" checksum="816A2D1D2B76BE11" modified="2025-10-08" version="1">MADVFSSAFAAEQHTAWAASQYAWDPSQLQALPLAGSPASGSTAASSEHGSEGPCSSAGAQRDQSLGPIPVGWQDTTRGGTAGGTAAGKEMKGAHTRAPKGPLVCQVDGCGHDLSLEKGYYQRYRVCEPHMKLLSLVLNGKACRFCQQCGRFQELTEFDGNKRSCRARLLQHNARRRKRDPLETSRRDISGSRKSKSHHPWSDSSNDADMGHGSGELSNGANDKGMANYSTDNGADQGIKVRVGVAPVSSSGMSTWNPGLIASVEAPGSVNQLHRMLQQPLAVPVPPASIDADLARCFSAGSDVGDALDEMLDGILNSSTDYLEDPRAAFLPVPPSVPPVLGGPFGSAGANMATGPLSGGSFTSGTEVKPLQFSMANLGMQGSPAISGAMVGAGSLASSVATSGAWQPGGMVLDNMGSAADLAAALNKRVAQQRELQEHQLLQQLQQQQLQQQQQLQQQHKLQQQLLLQQQQQQQQQLQQQLLQQQQQLNGVIARGPVNTAASSSMMQMDTFMDSTIVPGGVDNTISGGLIGQMNGTLNRSLGAPWPGSNIAVSVPEVMSSMSMNSNMSHASIGMSGMGPSGSILPSVTSTAASGSSSFDVARRLVASGGAAAQYQSEDLLIRVSVKIANCTPDQLPVDLYQRLQNLLNTADASLVQGFLRPGCTHLVLDVQVDSVARASNFGNDAPGGGASSGLAVSTADLHGVLGSAMRRCAILVQTDREVAMWPAAAPAGSKPQNSAWIGDLEAAGELPVIQHVSAGAHIDADGCAVLLVYGVGLTRPGVELCARIQGGYVPLSLNPLMEAGRIATAAAADPKLAAATARAVLFSKALERAGRKSEDILLVTVKDTPGTGLLLLEAQTGPLLSNWRPCLLSTDAAIATELSMCAGRTVRAGAVALASLEAFILDLGRVLDKRSYTTPLLRSWGVPDSCLACVDEADEAEADPSVAQDVCDEEKESNEVTLALVCVCERLLAISTDLGMASVSTFLAGALWQYDQDVQAILEAPYADGLAIVHRCMRSGSPAVVRALLAWIKVSGVRIDLATSVTLGISPLHLAALIPATWEVLAAVPAARKCWWSAKSLLDGATPFQLQQMLLSQLPRSALPQDVVNAFAPKPRPMPHWGTIMSTTETNPSTVPLVDVKDEPVTEQHSTGGSYSKSRGAVAVMAAMFVLSILATVVPSWLSGPKAAGNSHEASDNAALATDTSVTQRSFATFCSTIMYVSLLGLAILLLPLNKAAILSQFYPSSTCLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJT0</accession>
    <name>A0ABQ5SJT0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">VaNZ11_014598</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="19">
        <location>
            <begin position="38"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="207"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="471"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="570"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="657"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="16">
        <location>
            <begin position="714"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="1085"/>
            <end position="1175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="GLI69881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1184" mass="132943" checksum="A9E428466AE01E41" modified="2025-10-08" version="1">MYGADEDHVEPAPIDECEDEIVQEDSWNVITAFFDDKGLVRQQLDSFNEFVNVTIQEIIDETPEIVIKPESQHLPGVEAAEEKEYIIKFGQVYLSRPQITEQDGESVVLFPKEARLRNLTYCAPLYVDVTKTEYTRLPDDTVEEKQDSLSHVHLGKVPIMLKSERCNLHEASTEQEATNLGECPYDQGGYFIINGSEKVLIAQERMANNHVYVFKKSQPSKYTFCAEIRSVVEGSTRSASSMSIMMLAKGKKAGAAGVIRAQIPYIRAEFPIFILFRALGFVSDRDILEHIVYDLDTDPAMLEGLRASVEEAQEVQTQEEALNYLGKRGTVTGASKADRIQYARNLLIKELLPHLGTEEFMETRKGYFVGYMVHRLMLVALGRRPEDDRDHYANKRLDLGGPLMAGLFRLLFRKLCKELRMQAQRMVDAGKEINVLSAINEKIITRGLKYSLATGNWGEQGKQGIRAGVSQVLARLTYASTLSHLRRVNSPIGREGKLAKPRQLHNSLWGMFCPAETPEGQAVGLVKNMALMCYITVGTPSLPVVAHLEEFNTEALDEVTPADIAASTKVFINGVWLGIQRDPTALIKNLRAMRRQVAFSTEVSIIYDGPLKELRIFTDYGRTSRPLYIVEANRLLVTKNHIRRLISPLPDEEEYAWPQLVEDGLVEYVDAEEEETTMIAMTISDLRNAREAGSSTGTTYTHCEIHPAMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGMYATNYQTRMDTQGFVLYYPQKPLVCTRAMDYLKFRELPAGINAIVAIMCYSGYNQEDSLMLNGSSIDRGFHRSIFYRAYKEEEKKAGSLSREEIETPDPVVTSKMKHGTYDKLDADGLCPPGTRVSGEDIICGKTVTLPDDPTGAVQRFSKRDASLALRGHEAGVIDQVMLTTNDEGQKFVKIRVRTICIPQVGDKFASRHGQKGTCGITYTMEDMPFSAEGIVPDIIVNPHAIPSRMTIGHLVEALMSKVASLMGSEGDATPFTKVTVDNVSQMLHDLGYQRRGWEVMYNGHTGRQLRAQIFLNPTYYQRLKHMVDFKIHSRGRGPVQVLTRQPAEGRARDGGLRFGEMERDCIISHGAAAFLKERLFDQSDAYRVHVCERSGLIAVANLKKQQFTSTIYKDGSNIYQVYIPYACKLLFQELMAMCIAPRIQFDREPTPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJX3</accession>
    <name>A0ABQ5SJX3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ABC transporter family G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014642</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF91">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="520"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="632"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter family G" evidence="8">
        <location>
            <begin position="348"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="32"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="158"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="82"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF19055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="660" mass="69131" checksum="EDD5122535350957" modified="2025-10-08" version="1" fragment="single">GGGGGSRSSGGGGAGGGAATLANADAIPTLMGPLTAAAAPPPADAELQQHQQQPQQPQQQPQQPQQVDGVDGEEGREAYTGDAGADMAAADAAATAVSDAEASISGKTVPDGGPTDGVGGVGGVGGVGDGRGTPKQERGPNMDPMSRVRLHELRNNSAPLPDFTIMGGGSAASSPQGVGRQRRSSFGPVSGASPCRKQRRHSWLQQNSDGSGEISSPGGGNRKARKDTLYVNNLLSNALLGAWAATQSITQGRNNTPLGRLPRGIPAIATSPSPPLASSIAAATPAIADGNSPLAYTPGAPSSLNTPVYGALRTAAINRSSVTPTNGGGSGATSPIAGVPLNSALANRLDMEEALASRKSQHFTTQMLFLLRRACIKYVRSFWPMRVVDTLLQLAAAFIIGLVHGTRWSITSVPGNAVMCMVCLGVLSCVTHLRTFSNNRVLLWRECASGMSILAHFMAQNVMDQIWVVAAPALSLGVYYYLTLPRMPFAEFYVVGLFVCWWASGMAYLVSAVLPPQNVLMAGVFISLIFGAFLNGLTYAVATSRGTLMEGIMGLSYNRWAMEIVTINELRNYQYDHRNTIIMMAKAIGLCGVDTKLADDGEPGISVGEALSYMALQDNFDVDRYCSSHKSVGYVSLLAMGLGFRVLAFALLRYNCVKRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKC7</accession>
    <name>A0ABQ5SKC7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015314</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046738533" evidence="2">
        <location>
            <begin position="28"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="567" mass="64283" checksum="E6756B2ABF683AAB" modified="2025-10-08" version="1">MDQSSFKSKWALLQILIILCFTSASLQQQFALTEALLSSPLTNRIDDQNYNILHSLQLISQAPCQIRLLNATELLYGASQLCHEMLNQFPTLNESNIELWLQKSLGFLADHAKGSKSITLPTNQSLSVPSAHLSGLPMVRRSLLAQLVHGWLTGLQRQLSTRLEPLLVQLISQMYDIHRDSVVHRGVMQYHHISKSGGTAWNQAARANGCVVPVTLGNHVRGFGDECRWLDPKVYGNMTGGRLILWARWGQYRRPALGQSCWNRFFNVAGAGMSYISNEYTLIGGREKGNFRGAHSCPQFVNVVTLRHPRRRLESALRFIQIYIRGYWRRDDPLHGTERYKLRFCNATADLWRALAPAVVDNYIVRSFLGESGFHTPPGLLNDSHLEVARDQLLQFDLVLDLEGGREENDRMVQQGLGWPAAWSKANQTLNSTLLYQSFHHECGMRPEVLQELQRSQKYDMRLYMFSWVLNQLDSVWLSASRALGLRPETAPGALEHGRGPQDIRCGMLWQGGNGSTLGRALDSLGLLERQAPPPPQSVRTDGRPRLRNSQGTRSRSARRRSEMRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKE4</accession>
    <name>A0ABQ5SKE4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation initiation factor 3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_015377</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IF-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.10">
        <property type="entry name" value="Translation initiation factor 3 (IF-3), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036788">
        <property type="entry name" value="T_IF-3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001288">
        <property type="entry name" value="Translation_initiation_fac_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019815">
        <property type="entry name" value="Translation_initiation_fac_3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10938">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10938:SF0">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00707">
        <property type="entry name" value="IF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55200">
        <property type="entry name" value="Translation initiation factor IF3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor 3 C-terminal" evidence="5">
        <location>
            <begin position="174"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="259"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="262"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="290" mass="31058" checksum="38AC71CE825940C7" modified="2025-10-08" version="1">MAVQAIFLRPMQCPAALLSSIWQLANFDLSNFPGVNSPPGCLTSNVFTISSLSGSHPGQPGAPQSYLRHSVHHSRIGAPIRKLSLAATAAICRVPCPGQQQCRVSSRCPGTLLPFRVAYTTWRTGFAASSVNAKRASHLSELEHNVADSGRSACSLSASRAGGVSTPRPKAEPVKEMHLKPRIAEHDLGYKLRQLEGWLADGIRAKVVVEFRSADQSDAAAALVDSVVSRLAGKGQALGSRQQNKNTWFVVLRPPVSKIPKQAKQQQSQAQQQQLKGDTERQGSPTATAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL51</accession>
    <name>A0ABQ5SL51_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_015312</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the histone H1/H5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF36">
        <property type="entry name" value="HISTONE 24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="8">
        <location>
            <begin position="24"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="95"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="156" mass="16896" checksum="E73FC9EEB97723E3" modified="2025-10-08" version="1" fragment="single">MASDAPEVKAPKVKTEKKPKAAPTHPPYIEMVTDAILSLKERDGSSLPALKKFIEAKYGKDIHDKNFPKTLSMALKTFVKNGKLVKVKNSFKLSEAQKSKAKAAAKPKATPKKTAAPKKAAAPKKAKAPKKEGEKKAVKPKTEKKAAKPKTEKKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLX4</accession>
    <name>A0ABQ5SLX4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="684" mass="71561" checksum="D6C852A572245A23" modified="2025-10-08" version="1">MARIMAKAAVAVALLAIVLQYFGIMPPFPVLVKTFFPSLGRAATQTINAPKLLADLNSDILEASIAATKSTGSSGTGTQDNDQLARASSAALSVMYLASLPGLRDQVKQTPGIVESLVRRLLTLDLAEAHRDIKRQRKQGRRQDQMDLLSAALLATAKVAAGDRPLQDWLLGADGIGDPGGGRSAETRVPRGKMLARLGEVLQSGIPAWQAAAAFLLHSLSGEGVDTPVSSFPAAFQMRPNPVVLNWVVEHAQTLVPIGKSLVEAGAHFKNSDTVLAGSQILQLVASQPRGGRVVLETNLSSLLMQVLVSNAGDNSSSPMVGGAVRLLHRLVAPVSSDAAEDADPGEERKAAAVAQLIRNLTTSGLVAPLVREIRLGQVASLGPSLELLMELIRKDNSGGQEVAREAVGAGALSLCTQLLFQASSDANAHGACCNLVLHMVAHRKASKPPLGSGTPVVDKDDADADGGDVSGEGSGSTTGMVCDRSEEGRMALARELLKIMRQTDFADLHNSVLEACRDVAPSRPAAAALVEAGIIRHLAVILSVNTTKTREEGSYDMSATAWIMQSQRSATALVALLALQGDLRERLMAEGALAGLASALRVHSGRPGIMAQVRITAAAALVPLLGDDRRYLRLAVASGVLEPLLDMHSSGLAQEVTVSARVLRLLALDEAVAEALDNLGVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMS4</accession>
    <name>A0ABQ5SMS4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1499 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010865">
        <property type="entry name" value="DUF1499"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34801">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34801:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07386">
        <property type="entry name" value="DUF1499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="24248" checksum="F59A19E7F9E5B8E1" modified="2025-10-08" version="1">MQFQRSPRCGTIAQGMGISNFPFIIALVPRRLVAQVGQRHGDNTPIDKSKLSSRNAIVPAALFKLGRAIPRPVLGIQDYGAGPMLSLCPNTGTCIASFDEGHDVKYAPPLIYNPPDGRGSHAPVSQGEAMVELLTVVQELKPSGYTPTIVEQTEDYLYLEYSSRIAGLIDDVEFWFRPGPECLVEYRSASRGPAVDANVNHKRILAIRTALEKKGWRSRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNW6</accession>
    <name>A0ABQ5SNW6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_016612</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF25">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="469"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="198"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="366"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="824"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="260"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="828"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI71474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="845" mass="88960" checksum="DB684E97F2A5B098" modified="2025-10-08" version="1">MPLSQDERQGLSRECPSQPAMNSRTSASSDTFPSLSSGIGADGMGLRQFESSLDRIVNGGRSQERADPDTHVVAYNGRFQFFHTKRSSTMNEPVVNDLATREDSLVKAWRTVSEIDALLGSDFRPSVPSCGEERPSPVDEAYVKRLNSELEAAMNVSLTFKERYRAGRVGTRNGGGGGGGGGSWLGFVGVGGSAETWEQGHAAASSTATAGNKAATGEAQADVGQGTPHGATPVSHFHISNPSPRQRHSDASGIASPHSPLYNQQTQYQHQLQPHTLAPNSPRLSPRMVTSGGGGMLTVPSPPPTSSQAGAMTSTCASTSTKISNGSGVSVPVMATATFTVTGLEPAGGNKCLPSYSSVHIPMPPGWQGPPPSPRSVQMCGASTSASTGSAAAPSLLPPSASAPPSAVALLAAAVPHPLRRLHGGGIRVAVGSALDHGLWDSDGVWRPADPVHEVYIPQNLQRINADDLVKVRELGRGCFGSVWLAKWRGVEVALKELLNQTSLDGPPNEVFYEAERLASLRHPCVIAFYGIVATPGCYATVVEYMKMGSLKSGLSRLRKQGANISRRLRAAIALQAARGMEYLHNQYLVHFDLKCDNLLCDLRDPARPIVKIGDLGLSKKKKDSFVSGNMRGTLPWMAPELFPGVRGKQRQEAAAGAAVACDHAGVVIATAAVAANLGSGGGSIDGGGLDGDVNGVTDIAAITTVTVASNNNIFHNNGNTDCLDDRVNEKVDVFSFAIVLWEIWQLGEQPYSSYSLADIFAGVMTGSLRPDLPSDCDPDWATLMKACWHGNPRARPSFGEVAERLEDILERLTKQEIAATATNVGGDHFGGGGGGLPGPGSRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP58</accession>
    <name>A0ABQ5SP58_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_016474</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="5">
        <location>
            <begin position="40"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="612"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="678"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="478"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="613"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="626"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="636"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="685"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI71315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="786" mass="83214" checksum="3953365D82FBFD26" modified="2025-10-08" version="1" fragment="single">IRQFTTFLYFSRQTNQLILYPHLAAVGFTQTPSEMMAESNELDTEEFCYICFEGATRGPSGELEPLVQVCRCPTKVHRRCVARWQLYSAGKREEKSCRFCQGTLPDWKEVLTPRALPPAVPILSIYYNNTCCRMKVRPGPDGLRAFLRQLEVIVGRDVANVNFVFRCRCPDTGAEIFLQGLQAFEAAMHCACVRAAQRSLQQHQHQYQLLLLQQQQQQQQQAQQRQLLELASLHQHQHQREEYEHQHQHQHQHQQEQEQPLPLQERRRQQQLFLLQHCEVLQRQRQRERNRLQLPRQLVLDTGEQQPTSYGTATVDETARIGDSQVRRPSATAACIFGGGAGAATAITTGPTTTAVAAAYVPPPVHHAIGLHSSALAVLPPAEPHQQLRGAAAAAGAGTRMGLGTGLPGPEASSTAVASIEQQSLPHSLDPASAIVDGAAGRDCRHCWGSDISVAANCAACVGAARLTSAATERTAPQQPPASIPPVSPSSPLYGAVRQEQQVPAMMPLLPPPQSPSMLPMHCRESTPAVVSARGLDNGVFFRPIASLSGALHISGAATAPVLPPVPDATAPNGGQPSTIPVGGGMRASAPPSAPPPLDQAPFMITPVTAPKPASPLPLTTPPPLLSSRPPAATSYTAERNTGVASTDGTAIGGLPPCCLKIASDGLEEATSQAYAPVGSADLMPPAGSNGSPLNSSAPGGSPSAPRPTQPSRPLSVQVEPRVAGGAESPMMGTASCLMCSVGFGKGSVGGVGRAGRGKLSAALRGLKALKIALSRKAANAAAAFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP63</accession>
    <name>A0ABQ5SP63_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase M43 pregnancy-associated plasma-A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_017132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M43B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000120">
        <property type="protein sequence ID" value="GLI71787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47466">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47466:SF1">
        <property type="entry name" value="METALLOPROTEASE MEP1 (AFU_ORTHOLOGUE AFUA_1G07730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="698"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="800"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="698"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="838"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="857"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="889"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="921" mass="100242" checksum="6BB4041C2D12106E" modified="2025-10-08" version="1">MAFYNRLAFHAVFHYLVLHAFLRDNAHVVRASNTGQQGSGESEPPFLVLFDTVNASSIEVQKLFEQASNVSAVAYLLLGAHEPLSSAVGSSNALAPHLTPLILRLYILHPERANSSVAKALYKVSWSLHGSSPTLQACFRDMYNFLGKVFTWVRDNIPLRLQEVPGQPKASAMWPDIPPLELTAAVASALSSSGPLNDTALLISSSCGLTATAFAALVPNISSVLMDLHKDLPILQNITKNMYDANWGTQQSYQDNATNVLQPQARRRLLQQIKYVDAMPPGSFRPAAPATLPNVLVPLVFHILLYQDNLPHDSIGPAQYDQAPSYINRMVRQVNLMAKPTNFQFVVKEVRNDYGKYGYLLLGDRSTWLKAPYCDSTTCSTNLYKLAESYVSDWPRSINIFVASDSASGGSVLGYAFVPASDVYPNLGFVFLSWDSVTTNGGLNSPFYYNYGAVALIHEVFHHLGLQHTFGSSTNATCLDDDYVIDTPATFGPVSSTSFIVTAYNYCLDIIWGQYGGNWDRMFEAASNRLGIPEADMNSWADSCPGNPGYDELGNYMTYSIAACYPALGHLTPGQVQRAHYVTAEMNPILYAWGQYYAATVRPPPPQASPPLPDDPIFEICKYSNTLCKCKSKWTFNGTQYSYCSVTAPNSRLMCEVADVASCPVCTTVGLLPCILNCSLAATPQICNLTSPLPPGYKAPPPPSPPPPRPPSPPPYPPPPPPQAVPEECKFSSSGCPCRSTWTYYSSSSVSYASYCSNPDGSNRLWCQVSSTCPSFADSPFQYCPKGITKRSCNTSPVVFSTTRIPPSPPLAPSSRSPAGQQPSNPSTPVPLTALPPWKAPLKPPTPPSHNPASTQSPPPRRPPPTSSKQPPPLRPPPPSQPLKRKAKPPPPGKMKSPPPPLPSFLMNWSPPPPPRAKRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPR2</accession>
    <name>A0ABQ5SPR2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016596</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="125" mass="13897" checksum="9EF912D070B6CD0B" modified="2025-10-08" version="1">MLSALCGWFPRAGRLGPSYPVTRTGNPCECLGSVPTARGILGGHGEMSAVRMTERKYTYLPTYLPTYLCRYLLDQATSSTEVVDILTSLRDLLCVQFMFQSTYRGYDVGKLVLGEFCHPDYPLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPU2</accession>
    <name>A0ABQ5SPU2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016588</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="622"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="643"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="715"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="237"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="515"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="752" mass="76022" checksum="D9224A302A9AB6F8" modified="2025-10-08" version="1" fragment="single">MALVNTRAASAAVPVYDTNSISRSNSSGSGNTLKTGSGGGNWGTDAGGTGVVPCNGQPPVAAAAATAGFSPVVVLPALCSSSIDSAHSVVSSLASHAQELARVASSCGGGGGDVGCDVGGGDGGDDGIRKDERTPVDDDLNSVTLQPGAASASLTFPGAAVPLAAAATLLPPSQYQRYCARMCEAHAQAMAELASSSSTRVKNGNTDNCVGVVDSARRNASDSDLWLAAVSAANNAAGDGGVSQMSPLPPPPPPPPPRPGGGAGGYRQRTHGGDGQCWTSLDEWRQRNLLEIEATEGLVPGFQRSPPPPPYTHVQRHRWYDAATPAPLPQPLTGLRQSLSAGRNSYFTAASAGGGGSGRDVFALPVRMPASPPSNGGSGLSPAGKMLYRFLNQRATGGNGSGIGGGGNFVVTGEGYSGGGGGDGAISCHCDGGGDADEPNALTKADAFAFSPMTALRDADGAAVLESGPTAGSASPPPQPAATAATTSAAADFGAGDSSDFWFANPYAHGSDMYDTYKQRSPSPPVNKAHDGTDLSSLSYLPHLGFREGHFDLPQGHGGLPQLDGLGMPYGAAPHFQMYGGSNPLGSLNSNAPASPPLYCGSIGDRDGRGGRGGYIPSDYGGGGGSSVGGRSISGGNCIRLESLFGRLVSLSCDQQGSRVVQRLLCAAQSEQVGRCLDELLPCVTEVACHPYGNYVLQQLMTVGGSRHKLRLCGALAGRILELSFDPHGCRVVQKLLEVVPEQQAAAAVVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMW6</accession>
    <name>A0ABQ5RMW6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016833">
        <property type="entry name" value="Put_Na-Bile_cotransptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640:SF14">
        <property type="entry name" value="SODIUM BILE ACID SYMPORTER FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 10 MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13593">
        <property type="entry name" value="SBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="476" mass="50557" checksum="27A338192416FADC" modified="2025-10-08" version="1">MASTSSNHAREKVPLWKKRLGNVQDFVVENYLPIAFAVALTFALAYPVPGRFLVNIVVATNVHIIQEINMAIVFFISGLALNTTELRTAMRRESWPSVVFGFLMILLVTPALGFAMREIPLTPPAFAVGLTIFCLVPTTLGIGVALVRSCKGNEAIALLLTVSTNMVGVLTMPLYLKLLFLNFDTGGLKLNINIPDLLLKLTLTILVPSVLGKALREFFRPVEAFAKKYRTHLSLLSTTNLACIVWQTLSGARDMLFKQRASMIISVIVLSAAVHVFYLIVNHIIVKFIFRPPLVEHISVVIMASQKSAPVAVTVISYVTSIPAQQGLLAIPSVVGQLSQIFIGAALAKYIASLVTRTKKAAEAAATVNEAGGGKDKDKDGSEPGLSKTCSTSYADTSEANTGSSGGGDGGGGDSRLQQQGSCSRVLLSAARAYSGSCTSPRSQPQAVDAVPGTLPLCVVVEPRVGGGSEVDGTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN95</accession>
    <name>A0ABQ5RN95_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000547</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="269" mass="29201" checksum="22626435F8437BE5" modified="2025-10-08" version="1" fragment="single">DASNGSHASMRLQLISNLINLAQVSRRLPRVRPAETFAEEGVRSQKQVAVSRTLIAKKDHEAQVASIAGEIMKLTEQECQVAGKGIVAFDCVRDGWEANTFHFWKPLTRHRERYVSTEAFGAHTTAPRMVELLEKLQPHLEKPIGMSLYAYEDGRIGPVAIQAGPKGEGGLDDATGASGAAGGASYKQTSGAFDLTKVDEHEEARREQLLLLSMGQTAAGKEDGPQHSVENADEKVKARNPLPSLEALLSFANTVKESVLGPLCKLLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNH3</accession>
    <name>A0ABQ5RNH3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="16">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">VaNZ11_000763</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="16"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 16" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="325"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="740"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="821"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1000"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1033"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="18">
        <location>
            <begin position="40"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1071"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1074"/>
            <end position="1098"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GLI59079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1120" mass="123611" checksum="9A5D0F7D0B175019" modified="2025-10-08" version="1">MAAVGKGDVEAPLPATFPYDVTEEQLLAMNEAKDTAALKDIGGAQGLAKKLLSDLHKGLDPHGQGLASIEAHAEAYGINRFAEVPPKSFLALVWGNLQDPVIIILILAALVSTILGAAITEQRKHGEWIEGVAIWVAIVIVVSVSAGNDYQKDKQFRKLNAQKDVIMVKVVRGGHTELVENTELVVGDVYLLDTGDKVVADGICFDSQGLVVDEASLTGESDPIKKNPEDDCWVRSGTQITEGSGKLLVVAVGENSEWGKTMALVGEAGDDETPLQVKLTWVASTVGKIGFGVAICCFAALLIKWCIINKGFPLKKINQNGPIQFFLYSVTIIVVAVPEGLPLAVTISLAYSMKKMFKDNNFVRVLAACETMGGATAICSDKTGTLTENRMTVVEGWFAGKVFDHCPQPEELPRDVCDELKLNCSLNSKAHVLEHGNKIDFVGNRTECALLMMVKNWGCDYANVRDEYDASVYKVFGFSSTKKMASVTIKFADKFRHYNKGAAEWVLKRCTSMYDGSRIVEMTEVERAKLLETVTSMAKRGLRCICLAYTDYPLVDDSRPQDFFEDSDYLDRNLVAMAIVGIKDPVRKEVPEAVRVCQRAGIVVRMVTGDNIHTAQHIARECGILTEDSIALEGPDFRRMAAPELLPLLPKLRVLARSSPEDKLTLVMMLKQQGEVVAVTGDGTNDAPALKESDVGLAMGIAGTEVAKEAADIVILDDNFSSIVKSVLWGRSVFTNIRKFLMFQLTVNFVALVIAFFGAVIDGHEPLNVLQLLWVNLIMDTMGALALATEDPNPELLLMKPYGRTENLITRIMWKHILVQGFYQLFWMFLILYGVPTFFKAAKFNHRYDIEPRQDFWLRECMNDLNDGSYKSVTQMCNIMNVCGFPYNKGWLGNDLFPPNCVLNDLWRNRTTSGLDIEDARTAMCDYGSTSGAKCDLYTSWERAEKVMKDGYETHTDDEYRPTLSILFNAFIFCQIFNEINARRINDEYTIFVGLFRNPIFLGVILITVVFQIIIINVPFVNSKFFKVQRLTGLEWLITVAIGFGAIPLSFLTRLISKVLPENCLRGTRVHASTGRSLSGKPNSRTVSGASRTTSGARTVSGGRAASGGVQNHYPKKSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPM4</accession>
    <name>A0ABQ5RPM4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_001280</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16147">
        <property type="entry name" value="G6S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF8">
        <property type="entry name" value="SD21168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046063944" description="Sulfatase N-terminal domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="7">
        <location>
            <begin position="25"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="445"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="806"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1001"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="809"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1001"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1107"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1231" mass="131838" checksum="E4DFF615E2F80790" modified="2025-10-08" version="1" fragment="single">LKQMLLYVSIFICVAIASGQQFSRPNFLVIVTDDQDYMLNSTHPAYMPMLNQYIVEPGLQLRNFLISTAACCPSRTILMTGRYTHNNNVTSNIEPHGSFWKFISQRLDDDYLPVWLQKAGYRTMHVGKFLNAMDPTDPRFRCPQGWDTWDALVEPYVYLYNSPGFSTNCGPTEVLENQYSTDVISDKADKRIREAVAAGMANGAKNYQPFYLQVTPIAPHTQCDYINNAGGCVFPVPAARHKLMFSDALLPMNPNFNVPPLPELGLANEMMKSSSGVQKHYLARLRTLRAVDEMIGRLVNTLSSLGQLSNTYIIFTSDNGFQLGNHAQSSGKQFHWEEVVRVPFYIRGPGIPAGYVTDWQGNMVDIPATVMDLSGAGVPDVADGEAIPFKNLLPSFSYPYSSPTAAIKGRRRLNSNSCSSNSCSKSGGSSDRGLLYVESGFEGPDLLDPNSNSDPHLDEQANSGRRHLAQQTTSYSRGLREMMPIEMWGHAWDRRITMKDYRAVRICTGVLAFGSLQDYRSALPNGTFGWADTFYGSIGRPFAGPGLNGGINAYDNSFVDDNSTDDSVPDSRRRRLLGVADGTPVVIPDALLDPNPHSDMASVSPRAALMEAAQEAQVRRIIDAISKQLKMMAASAASVAPPAAAVAALAAKLPPRPYSLRLIFSYGEALDTDTWSKQLDSTCIYLTAATGAESCTANAATTYLAANGLSSMFDAVLVRGAAIPTTDFSAAIIEFAHEPSLFLPLPYRTLYNITALSAINCGIASGSDISAACNAATPLASQDFASSTRGVRHRFLLQAAIAVAGTPDELPPAAPPSPPGAPSDPTPMQPPSPRPPRPPPRPPRPPRPSPPPRPLPQPPRLPPNPPPNPPSPPSPPKPPNPPSPLPPAPPPPLSRVVRMSISYRVAFDLDRFGPQLDSTCLFLRIYASAMSCSTGKIPTGASLSGDGLSSWMLLTLVQTPRVSAADFESTVNDLQTNTAYYFGELYRKVYDINDITIILMAPPPPPPSPPSPPSPPPPPPAPPSPSPPLPRPPPPPSPPPPSPYPPVPLPPPPRRPPTPPPPAASPPPPSHSPPPYTGRPPPQKQPSPKPIGYPSSPFTAVSTFDDPPNPPTVLLPPSSPPYPPPSAPSPPSPPSPPPPPPPLRLPQFPPPPPPPSPPPPSPHPPSPPPPSPPPPSPPPPSPLPPSPPPPSPPPPPKPPPRPPSPSPLPPPYPPPIPPSPPPPSPPPPSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPX8</accession>
    <name>A0ABQ5RPX8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_001396</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11 12 13">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040053">
        <property type="entry name" value="JOSD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006155">
        <property type="entry name" value="Josephin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13291">
        <property type="entry name" value="JOSEPHIN 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13291:SF0">
        <property type="entry name" value="JOSEPHIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02099">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01246">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50957">
        <property type="entry name" value="JOSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Josephin" evidence="8">
        <location>
            <begin position="2"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="117"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="272" mass="30247" checksum="3D8754C210D027AC" modified="2025-10-08" version="1">MDYPSYHERQTLQMCLKHTLNNLFQHEAFVAADLNGIANTLTPPNIIGLSPHRIPIFGNYDVNVLEVALQRYDKVLTWLPQRPQVRKSPSCSGPQLPRTCALAAGASEYDCRKLHVDKEEEQQEGDRKHQSQQDEMQRRHPHPQGASPQQASNTPRTGTEPAGTRRGSGGGATSSPGLWLHDVISRPDLWALIINVAHPSAFPPWNWLLAGLGAGGRHWLGLRRFGGVWCNLDSHLPHPQPFQGKEAVCEFLQRALDEKEATILLVTAAHVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ83</accession>
    <name>A0ABQ5RQ83_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="295" mass="31318" checksum="B8B2DE1ABB5FB445" modified="2025-10-08" version="1" fragment="single">LKRSYHIKYRIFTCTDWYFKLQKFVRLVKMLSAALRGTPATISGQPCPIQPRLLPALPQSQPASRALASSYSSLTDEPIFESPRGQTVGISLQSHPSAHMEGDLDVDGSSSSDELEGDVFGAGGDLEDGPSVSSSQTQHAHHLCFSTPSAAGPMTSSAATSTAATLLSAGPSGEFTIGRAAMPTKGSMSDLQAELEHLTSINGGRGSRPIGIPRAGAFAPSTYQPVPGAFMQGFVPPHELAAASVQELDPMAVHSHRHKSIAERLRTAVFEQTGHMSRTTAAVFLQPGAFHIKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ90</accession>
    <name>A0ABQ5RQ90_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001051</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006519">
        <property type="entry name" value="Ribosomal_uL11_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01632">
        <property type="entry name" value="L11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="6">
        <location>
            <begin position="15"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="5">
        <location>
            <begin position="78"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="16419" checksum="B17FF483DE174C8F" modified="2025-10-08" version="1">MAAKSGGPRTLTNTIKLLINAGAAKPAPPVGPALGQAGLNIMAFCKEFNAKTAGFKEGTLLRVKMRVFSDKTYEWDLKMPPSTWLIKKAAGLARASGTPGHEIVGIISLKHLYEIARVKQRDMPGVPLQSIVTSLLHTCRSMGVRVVARPEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQP8</accession>
    <name>A0ABQ5RQP8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucuronosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001737</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF145">
        <property type="entry name" value="FI06409P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046537317" description="Glucuronosyltransferase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="587"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="305"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="631" mass="67265" checksum="3E002EFE80EF37A6" modified="2025-10-08" version="1">MHIIFLLELLLLSIFVHVNAGDLRPSLLVFTSVHREQALDLWALTEYLVAVRGYETVVLAPEPFAKDFRQPAISEPDELGREECRVGSLALVTYPSIAFEEYQVDSRGATHELTGVLGFHLANCASLLTNRTLIGSLSKREYTAILGVATDPCSTFLADKLKIQRRAAYDDGAATSLLWGILLGQGLPTSHVAAYGTRLGGGGMSFFQRIRNVLQYHRLQLWYKRVYEPRLAAFRQRHRLWALPAPLVPGSEGGEGEQTRGRGGVASGKPACHLRGQVVITGGDWGLLEPQPLPPSVKLIGPLRAQPAPPPTMPAPAAGAGAEGTTPTEAGETTGSGTARLPLLQPPELAAHVAGAGTGIVIICFPPDYRLPLRALLAIAEAVIDLKQRIVWQASEADIARVKQLYLPLRHARHVLMVPSVPLRELMVLPHVVSLITAGSVQAIYTAVYAGKPVIAVPLNAAQEDLVARLAALGAGQRLSRQELEGGAASYRVSVAIERMSNVYSFTQSMQLLSRQLRATLGSRSSLERAAEWVEYTTSLDEFGRQYLVPRSILAGSNPGSDGGGGGVSALGGGGGCMSPLEAANAAAYGVLTATGLGAAALIVLVVHVCTRVPPPPARKRSRAVLKAKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQT7</accession>
    <name>A0ABQ5RQT7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003882">
        <property type="entry name" value="Pistil_extensin"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01218">
        <property type="entry name" value="PSTLEXTENSIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1455"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1263"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="937"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1263"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1348"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1362"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1374"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1385"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1408"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1426"/>
            <end position="1440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1495" mass="154792" checksum="26389F03584A293B" modified="2025-10-08" version="1">MTRANPGCSLVGLGPKARFPIGGMASLTSHGSEAQCGQDACATTLETSASALQCLARLFPPTSCIDCHCHEVNALRQKCITSSGDTPPGCTAQPCGLDHCTAIRFVLTPKDCVDEGAYVSLMVERSLATSSCHTQSCSSAAGSCGSGKMRAIGRYCNSNSSGSETSSSNESAIGATGATARLNEWHPARDPSSTLPMQRLQCSGAVDMQPLRPPPSPPLSSRRPSCDQPYLNKYERRFTQSFGSRPTGPGVQGRLSLLTMLWVAWASAAASAVPAALGQVSGPGMQAGAVRQVATVHDNIGISQGAPSPSHAGPRELLQIVAAPSVPAYAQMPTAPPPIPRPPAWPTLPPLLLAPFAAPPLHTPLPMARPPPSRPPQALPAAYMPLPPLQPAPAVPLPPSLSEPPKNPSGPPVPLIATQPARPTLPRWPSLPRARPPPPTPSPPPIPIPRPPKPPRLPPMALPSPPPPVFLPPWWPELPPSRRPREPPRIPSPARPLEPTHSTFPEPTGDSPVDPSGAPEPPTALGELPPPLPPPATPSQNPAWGSPQPPVHLPSQPHSPEPSMPKIPMSPSPDPPNQPPPNHPPPSQPPPSHPPPSQPPPTSNPPNQPPPSHPPPTPNPPNQPPPSHPPPTPNLPNQPPPNQPPPSPNPLNQPPPSHPPPSPNLPNQPPPSHPPPSPNLPNQPPPSHPPPTPNPPNQPPPSPNPLNQPPPCHPPPSPNLPNQPPPSHPPPTPNLPNQLPPSHPPPTPNPPNQPPPSHPPPSPNLPNQPPPSHPPPTPNPPNQPPPSPNPLNQPPPSHPPPSPNLPNQPPPSHPPPTPNPPNQPPPSHPPPTPNLPNQPPPNQPPPSPNPPNQPPPSHPPPSPDPLSPDPLSPDSPSPDPPSPDSPSPDPPSPDPPSPDPPSPDPPSPDPLSPDSPSPNPQSPSPPLGPTLPSSYPLSPPPQLMSPPPRPPPVRPINLLPPPKPLPRPPPQKPPRPSPPLPPRPLPPRPPPPRPPPPRPDPPSPQPPPRPLPPRPSPPRPPPPPSPPPPSPPPSSPDPPSPQPPPSPPPPSPDPPSPSPPSPWPPSPAPPSPLPPSPQPPSPPPPNPPPPSPPPPSPPPPSPPPPSPPPPSPLPPSPLPPSPPPPSPPPPPPLPETQLTIEITSRPGGSISCDRIDESLQSITDMVGLRPLVRCSAQEVAGEPRASSILSLDTVENTKAAWEAIYDKISTVVLYGGVGCGSTVMMLRTSSTPGTPSTAFFSCGRPLPGYMDLPDLCCPPLPPSPAPPLLPPKPPNPTPPSPPPPSPLPPKPPPPSPLPPTPPSPNPPSPSPPAPWPPSPPLPSPLSPPIRPPYPWPPEQSQPDSQLPLFPPPSSFPPPSSPAPSRIKPNSTNSPAPPNPEPLSPFPLSQSPLSPSQQPSAPQLPSPSPSLQQPPSGTPSQGQWSSMQPPLTPSTFPTNEMGTLPGGSSGSSPGPIVAAAVVPTVVLAAGAGALYFYNRRRHVNQALDNLMGGGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR32</accession>
    <name>A0ABQ5RR32_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 157</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI60033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="30620" checksum="85A8B235ECECB60D" modified="2025-10-08" version="1">MFSCFGTSKLDALRKEIDALKSELESKDCEVDRLRRLDASQRQKEEQQTESLQAAAAHIAQLEARLSMERMEQQRISKENEMQLRSDYEQKLTDAAQQRNILERELEDVRQRLAEQQLMVERQRHQLEDTVLEQTQQLEKFREDTRELLEIRLLEEQEKLERLEEKHTIERTKLLRRLAAEEHRARRFQKQIANFTETMEEVSNILTAVENDQKTIASPVKVDKKAKASVAADQLRQSIALFKRTKDEQTLDQGAIQECT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRH2</accession>
    <name>A0ABQ5RRH2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rad21/Rec8-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002223</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF69">
        <property type="entry name" value="FI11703P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="4">
        <location>
            <begin position="1721"/>
            <end position="1759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="270"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="361"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="466"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="579"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="930"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1005"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1140"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1285"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1565"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="296"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="388"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="486"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="602"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1015"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1046"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1305"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1355"/>
            <end position="1368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1834" mass="184145" checksum="75052AC01DB1B32F" modified="2025-10-08" version="1">MFYSTQILARKGPLGLVWIAAHMDRGLKRSQIYEASIPGTVDVLLSPEAPLALRLSGQLLLGVCRIYSHKVTFLFQDCQNALVKIRLARPEQLAALTKAVAGAKGRRRGGAAGADAPVHTLPLESITLPDDLDELQLLAGDNFDLRPLQPGTALEAMLEEMQQGGPPGGARRRRASMGISLAVSSETGFQEGCSLGLLFAPSTHFTGAGVGDSGDVEMEVFEDAGAPFQFDLEEVEIERLRAVPASEASGGAADLAALLGGELSAGGRMRGGASIVGDDEDTRSGSPSSGLTVAASRERESAGLSVDRRKSTATAATMATGGRGAVKTSEPRLGELLPDLGLSAGAGDLDLDLAMDLGISDEQREGDDGGDATTPSKAPGVSGTRQRAAVSAGDAATGGDAAGAATTSALRTGIAAAAAVSRGVSFPVEEIRAAMEAADRAAARMDPYDLPHGADVDVVGHEGVAPADSTLAAPPKEDNVAPQDPLPRREGAEQTEQRGATPTPVYQDKDGIDAMEMDVGDGGAAAVPPTEGAMDAARTVYGAASLHVSIGAVQQSASQRMISNVQAEAGIGVEAGNSQARDAAGVHSLGSSGSHGPPGGPPSSAGGQAAGNDSAFQAVGPIMSTGAAMSSGGGGGGGGSAAATASINSGAGATALNEEEQAPAAVPAAPLPRMLTRRAAAAALPIEPVATAAAAIVIAAVDQGALEDQQSSPPQARALMGRRRGAHVVAGRSIELDEEGLTLKTAQLREWIQDRSEILDQTRLMKLSSSAAAEPTALRPRHHLQTAKRRRVATNGSFVALMHARAELEMNGVMPSGQDVLNSAESVAVGFWQPPMRDPGRSDVGRRITLQRSVGAAVPLAPSIQALFRVAVGQMAPQYALQMGAAAAAGPMGRARAPSGAGGCAPSDAGAAAVCPAPSWRMAPAGLAGAGTPTRGVGTTADVSPPSGNTMGVGGTGDNVVARGTPSMQRHGASPAQGLIGGASAGAAMPMEVFSAGTAGGSRGGVTASGGLLGRRSDGDVRGVERGDEEVGQGVEEEARAGTPVGRIGAALGRAAAGTSAGGASRQGSGHRDEGPAGSGRQLRVVLFPAREEEDEAALMMEDVRERENEGANLADGLHGGPAHAAVAAAATAAAAATSEVSFGHTESDKENNPVELNEDAGKEDLPPRQGALGTPAHRQPLALQQQTQQYLSTPRRATGGITAAASAGRMQGPGGRAAAAAGAASGASAAAAEEGSRGQQQEGVWGLDSGYGGGAVAEAMDVDGEGHNAVVRAEQGLAGGMDMNFGPADFAHGQIRSRGSQRTSAASAPSAQQAAEGSGAANAGAAAGAQGPAPQLATPLGSRRQVPGSAMRLRSQTRSQPSAQQGGHQHHAVGLVAGTPLGVGAGGAGNESMSHSEGLGLGSMLQRRQRPRFIDDDGVDSGGGEAGCGGSGGGYNGYSPGALSAGRSASQHGAGSKGRLCRSNLDSIQEEVQAGGTPVHLAAAGPGPGAAQPVSDGRLTSIDPHTTGALRTALASGSGLMSDVDWSREQDESLATRTFQAFDIVARAAAVAAVAAAEIDGARPGLVSESGGTSQQPYNEGGVSEADVLPLGLELLRRPFGCAFSRQSQRQFSQPVARKLHELETAGEGAAAAVAEAGTQHPTQLRSDGDSDGVDMGAQQWAHQTSTQETGGNVLPTQWRPGANLGGSQGDRPPWSQALLFRGASGSGAGGNQATGCAAGAVQLSVMTRDRRRKDAARCFYDMLVLNNRGLVNLQQDASARASRHETGVAAGATAAGPAADAGVGAAPGLNVEIEAGNVADDDDNVVPIVWVTLTQAGVEAARQRLLQRPAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS72</accession>
    <name>A0ABQ5RS72_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002589</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115:SF1004">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115">
        <property type="entry name" value="KINESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 2" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="4">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="388"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="437"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="606"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1020"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1062"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1126"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1211"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1265"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1353"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1456"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1847"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1898"/>
            <end position="1933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2067"/>
            <end position="2126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="502"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="528"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="553"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="609"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="646"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1024"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1265"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1353"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1365"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1456"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1486"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="2067"/>
            <end position="2078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2103"/>
            <end position="2118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="65"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2385" mass="244221" checksum="31D5B1D14F9D297C" modified="2025-10-08" version="1">MRARVSAFARWHSGVVESRKISVDYVALDKFRQEDLSQEELYESSVRSFIERCLQGCSGSIITYGAWGSGKSHTLGSLDPLQLEVATPASDSSGVLSRCTQQVYEWLGQDAAVEVTCVEVQGDDIVDLLDTRYNAAACCSRSTSTEGCNTSTNSGAVGICSPGTMLSSPHGSFSRSPIAAAAAAFRRKPLSITVARNLTEALLILKRAVSNRQTFGTSSRQRSSRTHAVFQLRWRNPTGPAVSGGVPTCGSDVAAGLPGRNPGAAAVHRAFSAPPRSSLTTTVFAGSEAADAGAPGSGAGGPVCTLTLVDTCSFSPLLPMKEGSAVKRSLLALSRVLNALQRHQALPLRESKLTRMLEGALDQVLLLACLCGAGDEPTVQLVKMLQQGVPRDSGLRRSRRASSSGSRHVSAAWESSASLGATNAVSLGGGTGSHPAPCAGSAGKLSARTPPKAPSAAACRTAKGPVSPLMGRGASRALFHSPVSPPSAPSHRTITLAKGRTEPQQASSTVANGYDSKEAVQGTGGDLQDWRDARGSDHVGEADSRNSSGGGATDTDAAAAQPVSGSSLDASSSSPAASTQSIDVGEGCGSVATSTTVLGAAAAAAAKTSRMSPLSVHVTSTDGPLVSPDGAGAHSGGGSERRTPLSAGGRRPGSSIPSPPGCLRVSRKLGSARQRPPSVTARASPGPEVRRSQGFSVLQGKWAPPPPPLPQRDTSFPIFPCLVQSGEGHVSLSIAMSSQRSSRAAAATSASYESAKSLLDSLRRSRGPLRTSLDLHAHPVQGSADLRLDMAEREMKATTRFRSRIPHSAATSQGVSGGGTAVSPFVITDVVFEDPESVIPDPAGHAHSLPASTAIPAAAVPSRTATVATESARSGCAAVVTPTSTRRKLARNLIRSPQPSPHSKFPVSTNTAAAAVTASTLRPSQRPPPRSPSPIGAATLISLAAVPPTRLASMRYGSANVSQDSVPDSITAAASAISALLSNSPTDALATSQVQMLLRNHEPAAATAGVPPAGLGLVGGAAGAGSRARSGPGAEQKRSVSSGLRWPSPMLRARREEWLSDLRRSRSAQSRRVVATPPHPAGNAVSPRQRPHGFRCSSTSRPTLSAQYKDLLLDFKAFKARERTPRTHCTSLGAAPPISPTPTAAVPPGSPDHPRAAQPTTHPDDSDVLPVAHGLVTKPTSVMVVVRANGGHSASGTAYTSSLVASTSASTFVGPESPAEMSTPSSAKAVSGSCARRSPPAFSRAHSPTNDEGLLSLTAAAAAAAASPRTPASGSALPSGRPRPGVSRVTCTMASPRPPRSPERPSTPISPVAVLDMSRLFVPKGSSPVAVAMTPQPQGSSRSRLSMGSATTVIAPGQLTSGGSERYRRLSGEDADSVRTSETEADMEADADRFPSEGGWYPSGTSESDRDNLLGPGSLGHLGLMLDAGGDPSTLCQSISQMASSGLTSPAAISTARKTTSARATPSARATPSSARRTAAHAVARQRTTPRNWNTDGDNECADEELPAVSFFIHRFTPRKDAQGVDPIAVQRPGSGSASGMAGAAAAVPGHGRCSGVGDKGAIAAGSPVSPTRRGDAAATLVQAALGVGTSCFTQTSTGAVRYRRTSAECQRPLSDPQLQLELQPELQSHQPRHLRFSATGRTPGAVAAAAAAAVAELAELQRLYMSRERASLDSGVLRGSCAASVGDPRSQAGEELERDQAGQLDCHAALHRRVEQMRSPGTMGAAVGKAVLGPPSHSSISLRQLLEQQQQTLQQRPGRRAGGWFAAAVGVATGSGHPVGDELKVLAQRRDHPGVLLSPPLAANRLLAQRAHVRPNHLDLDEHSNVLVDVDSASESDAVVAADTPAGRFATGQRNRRSGAGLGTVSPSAATGARVASPLRPGLPLPELPSSAFRVASPMKVSPSHSPQLPPPMSVSPAAETQSPVAGSNAMAPRTTQRILKSWTNEGGAVGGSQLTAACLPTAVVEQPALVHAAGPTDSVRNGLAQARSALSAALATPVNAILEMLNKQPLGGGTAAPVLQHARVSNDVRHQRHFSADSGPCDGVAGSLSLRIGLQGSRCPAATAATSVRPSAQQPVHLQGSLDEDVARQQPSPVHAPPSLPLQTAPQQQPPQSQQPLRGSGNVAAAWRAAAAMSSSSSSVRSCFRSSSGQQQHMLGSDATAVATVVAVSRAAPACHGDDKHASAAVNGHATINTAAAVKSSGVLEQLPNSCPTSTLITGAAIKTGTAAIAVAINATNPSSPPPLRLHHLQAPHAAHLLSAGACMQPVMLPVADEVVPAITGAAVPFSALAVAAAHTVTEPVTLPVKSKSARVGDRIAPLTARLRGCKSHRTPRTARGLSSAATAGNLEDVVVPQPVPPPAYPPGFISWATKIFSGAKKNKQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT48</accession>
    <name>A0ABQ5RT48_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytosol aminopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003014</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Release of N-terminal proline from a peptide.</text>
            <dbReference type="EC" id="3.4.11.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.</text>
            <dbReference type="EC" id="3.4.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homohexamer (dimer of homotrimers).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00181">
        <property type="entry name" value="Cytosol_peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023042">
        <property type="entry name" value="Peptidase_M17_leu_NH2_pept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002076">
        <property type="entry name" value="PRK00913.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF23">
        <property type="entry name" value="CYTOSOL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="8">
        <location>
            <begin position="486"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="637" mass="66024" checksum="B24F83AEB8927B02" modified="2025-10-08" version="1" fragment="single">LQAYIVIKLSQFHQFLSTGNVDLRSSLDAHSGMAHGILTRSARVTAKLAARARWLIWAPAPSIFGGRTTASVPLTSSNAHRVAPGFASPCGPIGIPRCVRTAAASAASIKQVTFEPDTLPFVGLSTSGLDAAEGDLLVLAVTEEEFATEGDVTSIKSEQLRQLDESLTGVLTEIVAWGGFDGKQGSSSRVVRHTGPGTKAKHVAMVGLGKGAKLTAAADWGLSPFQALGSAVATLAKTNKAVDVAVALLTQPDPVLREPALSQIVTGTLLGGYESSRFKSKKSPTASKLASVQVLMDLDAAAADAAVARGVAAARGNLVTRYLVEAPPNVCTPTHLAEAAAHIAAKSPDVFTLKVYEKDECEAMGMGCYLGVAEASAEPPKFIHLKYSPKGGSASRKIAIVGKGLTFDSGGYNLKAGAGSMIELMKFDMGGAGATLGAACILGELQPDDVEIHFIIASCENMVAGAGLRPGDILTAANGKTVEVNNTDAEGRLTLADAMWFAQEKCGVTSMVDIATLTGACIIALGANIAGLFTPSDSLAASLTAASKAAGEKIWRMPMEEEYFESLKSPVADMKNTGARYGGSISAALFLKQFVKEGVEWAHLDVAGPVWNEKLGLPTGFGAQLLAEWAVAQGVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTI1</accession>
    <name>A0ABQ5RTI1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SKI-interacting protein SKIP SNW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002562</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNW family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="3">
        <location>
            <begin position="171"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="542"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="609"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="380"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="557"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="630"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="664" mass="72055" checksum="4A1FC762BC693FC6" modified="2025-10-08" version="1">MSLLANLPAPTKQHAAPIQAVPASAAIVAMTYKEPPPYLRRQGWVPRRTEDFGDGGAFPEIHVAQYPLEMGKGSGSKGSQQLAVTLNADGQINYDSVVKQGSNRDRIVHSDHGALVPKVDRLSKEALTRPDEEEVEETIKETAAALERVVQGKLSATNPSKLPNQPGSSTLIKYTPAQQGAQYASGAGQRIIKMQDMPVDPLEPPKFRHVKVPRGPGSPPVPVLHSPPRPLSVKDQQDWKIPPSISNWKNPKGYTIPLDKRLAADGRGLQQTQINDKFAALSEALFTAEAKAREAIAMRASIQKELALKEKNKREAELRQLAMQARMERQGAGTAGAGSLPPPPPPLGGAAARADAPLPGPPPGPPPRGARGTAADAFDDFDRRGPAGDGNDRDRERDRDRDRDRERDYDRSGYDRERDYDRGRGGDDDRYQETAEEREDRRRRDEIREERRRERERERRLEAANQTGAKKSKITRDRERDVSEKVALGMANVGAGGEVQYDQRLFNQDAGMQSGFGPDDSYNMYDKPLFADRGSHLFKASRATADDEDVGPADVGPRTDRFRPDKGFEGAEPAAGAAARGGGGRLEFERQAAAAEEADPFGLDQFLSEVKGGGRERGGRKGNALDAIGQRGGMSAAGGGASFDEAGAGAGMGRRMQFTTGSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU67</accession>
    <name>A0ABQ5RU67_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="35"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="793"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="876"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="977"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1172"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1280"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1337"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1440"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="895"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1105"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1217"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1369"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1440"/>
            <end position="1456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1517" mass="151777" checksum="2B4FBC2B148B2D6C" modified="2025-10-08" version="1">MGCLFSKNGQVRTLSDIEEPLPASAFSTRGAAHAWLQEIGLPQYAEAFEDAGFSDWDLLSCLTDTDLEAITAHTGTTIPPGHRKKLLMASRQMGTSAAQAVLGLPPPCGSAQRYSRTSNPGNGNDAKGGAGTASAVINSLERRSNGLRPGGASRVPSSASVHAGHGAQGHLPPSLLALASGQQRNGTDDGLVSGRRSRAVAVMVPSRNSSRNSLLSTVAAAGYAIGYIASPSITGLQGTAGGGGCDPWIVLDGRCSSVEPGSPPAVAAVAAAPGLGGLTQPPRQRRRWSANKLSGRGVRAGSNGGGGGGVPFLASSHSWLEPETGGVVVMSTAGPITDDVRQISVTSSIGEVYEVAAGGGGIGGGPIPIQPCISRDLEALVMGPSGASNRAQSSRSQSLAATRPDKSYGGACSVSGRPSSPPHPPRRVSGGQVGAAAAVTGASANGDGGGSLQASPIMLGDLPYTRGSIRSPTAGAETQQRPSYPANDSLFQPSPDGYEQQQTPPKHPRIASARDGTAAVATGAAAANTWGTISQSKSQQRPGSAAADSTLRSWVSAATAAAAAPQQKPPVVLMATSRSPTLMPPGVAWASEGDAAISKGAVAATGTGTGVASQAQSVDSDGGGLLSSPSGRGVGNPGGALPRLVRAIAGWAIPSPVVSNTAPDGAATTGVVAAPGMSRAGSANRQRRGYTPQSVSQVMQTHSRSGCIDLAAEQASRERSPPEEDRPVAGDVLSEAAAPPLVAGSVSAARGLLTMEPSLCSNQERRRFQRQMIAPDVRTPRGPWDQNAALCIQGRGYKPSPSPPPPTHGLAMRSAVSSTQGGGGNNSGVGDVHSDAPSSPSHPISGAVIQDSAPGPVGLSIATAAAALHGGGWPFIMPQLPQPTKSGNGDVPGRPAGGHGGGEGTSNGVSQISNVSIITAAPALAEEEEDAPTTSGDYGAVAVSVAAGARMYGLPDSIPSMLLESLASDVSAATTARRAPPVHSSRPRDWCPSGDGGSEDAVAVAVAVAAEKRESSIDDLALGGSSALLRPHRVHIVGPVMATSYSAASACISPLRGGDGAGASSVQWPIIPLQLPKRSRLPLPPQAVAGSGSDHNQKLDVLPRGAPGPSSASAAVPQGATAAADGAASALDGAARPTARRKLDLEMLANQVGRIMSHLSYIAKDGAESDADVVSAEAPAPLASSGTKPATADNAGGRDGGPSGRRVVKSADGNVEPATTAAASTPGGSAFDAAAAATATSARDAPTPRADPRRQAIDRKHLELKQLHLDLVQRVAAIEAEAPPRPLPPPAGKIATGGGSGDGMYSAAADHTSAAADALAEVEARIRTKLAELAELTMRPASKRRGSKGNGSSGCRGSSPGTARKRTLSSDLASGNPSWNAANHEDGGGEGAANLTSAQRQRRAHEEAALHMEVLAVLGARKRQVRGAALAEPRYAAASATTVGSEDGSQPRQSPSPRFGSGTDANAGENRLGGAAAGGGGAVLADLQDGLRAELLAIDNKIKARVLGSGAAVFRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUG2</accession>
    <name>A0ABQ5RUG2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003572</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045355268" evidence="2">
        <location>
            <begin position="28"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="165" mass="17112" checksum="145D51E4F81E6ECB" modified="2025-10-08" version="1" fragment="single">MSSTVHPLCAAALSIVVFLAFTTGGQSFHEDDGGAEAVASAVIQAMGSTAVSHPAKNKGRSLSASLATVPGSWSEDVPMVSGDDYLNSPGNTYQLTLLGYSTDLSIVNMATNAVVWAISGLVSYPAGCYISKAMLQVYNIYIYMCVCVCVCVCVCVCVCLPGSCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV70</accession>
    <name>A0ABQ5RV70_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35046:SF26">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35046">
        <property type="entry name" value="ZINC KNUCKLE (CCHC-TYPE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="34"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="125" mass="13917" checksum="FE12182E3D88E408" modified="2025-10-08" version="1">MAKDTDNYVLSCDLCQRNKSQPGKPHGHLQPMPIPDAPWEGVSMDFIVVLPKTEGGYDALVVLVNRFTKMIPLAPTTSSCTAEQTARLFFDNVVRLHGVPKSMVSDCGTQFHLYFFWSTIVRSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV88</accession>
    <name>A0ABQ5RV88_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003707</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF18">
        <property type="entry name" value="ORC1-LIKE AAA ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="891"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1224"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1308"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1332"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1376"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1514"/>
            <end position="1810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1825"/>
            <end position="2670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2687"/>
            <end position="2748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="535"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="573"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="688"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1410"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1428"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1532"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1561"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1652"/>
            <end position="1661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1666"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1685"/>
            <end position="1697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1732"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1825"/>
            <end position="1840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1841"/>
            <end position="1902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1912"/>
            <end position="1939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1941"/>
            <end position="1955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1957"/>
            <end position="2036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2037"/>
            <end position="2049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2055"/>
            <end position="2070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2080"/>
            <end position="2112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2113"/>
            <end position="2123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2153"/>
            <end position="2163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2164"/>
            <end position="2179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2180"/>
            <end position="2189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2294"/>
            <end position="2319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2320"/>
            <end position="2330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2331"/>
            <end position="2353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2354"/>
            <end position="2367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2391"/>
            <end position="2402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2406"/>
            <end position="2415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2426"/>
            <end position="2445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2446"/>
            <end position="2459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2472"/>
            <end position="2488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2511"/>
            <end position="2527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2578"/>
            <end position="2598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2607"/>
            <end position="2617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2633"/>
            <end position="2642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2737"/>
            <end position="2748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2748" mass="287074" checksum="194A6125BFFA1638" modified="2025-10-08" version="1">MEPVNQFRAKVQQILDKECKVDWGKEGKYWGIISKYDATHPEAPWTIYYPRDGDEENGVFTPDFAEWHVEDEENPKDPFTVRYILTWKDERPTSLPEPPDELDNMEFEPILGRREELPHNQSSDSEGSGDGAGDNNESGGAESSSEDGVEEGPDGSGPGVRRPSSGGSMSRQLVVPARLPPKLSARRPSSKPDPKLRRSASASGLAPGGSQSQGDEQQGGKRKPGRPRKHVLPGGEQPPAKRLRGRTPKNTVAPAGPGSYGGGDNAAVKDKAPRMRKVTTAAPADGATGKPPTSDTIGSSGVGGGCSAGRSGSAEPATVSQKPRRLVRLDSDELDDKQQDAKLPIQKGPVKAAAAAEDDGAGQSPSSPSSDEGAPRDGGDATVSTGLKLAKVAASDAAQVQGQAAKAGTSPEGKRAVGAGRPKPLLSEEQLAKRKMSFGTAAPPRAGLLGVVPTARMQREAAHPGTVKLEGPLAKPTAKATLPKQAATGPGTGNSQLQSTVMMGAAAQSKAAPVAVSTGPAGSAAAVAAADGNNSAAADAPVGSAAGSQEAPPGAPSPALEQLDEHPKSANISSRSLSQQQQNQASVAGSSGTTVTPLAVLNQTLLPPSLLAPLSSSPIVSKVSLPSLPVVSLPPRITSSVSLAAVVSPHTLPAPQSASPAAAAAATTATTAGNTATLSASKADLQLPSQQDSETQDSQQQDLQQLPLPPSQWQGQQRGVSSQLSQRGPQTTAIGTLESPPPKGPKLASVTTVVRSAYLPPAAASTPVATALLATAATPAVVATATLPARPALPPTAALVALIPSATVATASLPAAISTTLPTATTPVVAAAATAAAAAATTTANTVAAIPTALPIAGGAAGGGSAAEALAAASNAAIASGATPETAHTDVQQLLHQLLGRALDALTNSQNAIKSTANSAILMAKAVGANRVARGFFSKLQLLGEEVAVLAPEGCTGDATGCERVAVVLAKRLNVFYVMDSMLQRSTKEEVISSWPKWVNSGLQASFRLKVFTEHHLKSVRKVTDVWKKRNLLDTKVLEMADLILKEQESMLKSRMATMAMGGPLASAGAATAGAVDVPVPGGIPAVAAALPQMHDGPDGAGLVEAPNGVSGEGPAAQRFGQGGAQAIRRRRQYCMVMPRPPTLARISYPMGEYEMYYINRFGPMTNRCYIENPYGSMGDVRDEATGELVAKLIDVDLSEDEDEPDFSGTVAWREVISVQLPQFNEDSPHRGGLPAAKVTPPHPSLEEELWRTALGLSKTPVASMRQNLAPKFLTPIPPGLLSGASTAGSVGAVVAPTSFAPATVPASAPPAVRPSGTAIVNLTPPANDLDDLDTWAYGGEPAPTSGGTAEVNAAPAAVPLSTAAAAASVVAAATSGSRSAEEDVQPNIYDMPAAPSHEVASTGRRADWEDMDMDMDTSENEDGEWQPPLPSAQPPPPSCAAPPPPERPAPVLPPATGLAPVAGLLQPSAPGIGLGAPDGLLAMMGPGPMGFPLGLPPPPFMMGMPPVPPLWPGQPPLPHPLTAPLQQQQLPPLPPHGPPPHGPPPHGPSPHGPSQHGPPLHGPPPHGPPPHGPPPSASPPLPPELFPAPPPPDGLPSPPPPLPCNPPLPSGGSPPPLPPPVPALPPLPPLPPMPPMSPPPPMPPMPPLPPSGSAHSPPAMDDMHPPPLPPDAPPQPHVTKAALPPSPIEGPPPLPSDGPLLEKSQHAPSKTPASIPPHLRPVPFTAASDKTTCTTTVPAAVSPQQFPSAAAAAKGVTPTAAANTPPALPAPEAFEAAVAAPAPQPAAADGLQQPPARAPVESLAVPQSQEGGDVVVCAAATATGGASGSALGAEQSSSLEDLKREREGDKVREDRDRDKVRDHDRDRDREREREKEIRERGKESRSREELERAKSGKEHKGKERGHKRDRTSRGGPEERQQEEEHQQEQQVVTRDRQLKLQVSPPSQAQQLSSQYWDRERERERVSEGDSRLDKDTGWERERERDRDKDRERDKDWDKDRERDWERERERARDGGRGVLQREADRERDPWERELPRQQQVQLPLQQQPSSGGSRDARRRYDDRDLRDWPPPKPHVGSPCDREWDPRDADRDLRDWDGNLIRIRERGGDTWDRGGPPRNSGGNRSRREPEPMRGGPPGPPLPQHSHPHLHVRGYREAEWDLPRDPLPPHMRGPHPPPTGPGRGGPGPGGGRPPSPPLHGRFGYRDSPVREPPPPLGPQHSGDYAAANGRGGRGPPRHSGGDPALDPPYREGPVVGYVRPPYNQFGDESAGGTDGRRQSIGGLKRPMSQASLPDDQSRHEPAVVQLDGDRDRDRDRDLRRAQRRERKRARQRREEVRDRQHGEREEGGFSRREENEDGEEGEEGELPEPEGSRSAGAVATKDNRERHVPHAGGSGGRGRGRPGGTEVEGEAEEGEVELPQHPLSRARQQTRPPRSPSISTTPSQRIHTSQFAPNPSSDSTPRAPDAAAIMALTSVSTNPAPSQLASTHQPTDKKLCQLAAQSHAHKGDAQEGQEPNQAQAQSQGQGQPPRKRNVITFNLAPRDSREGFTVTAQPLRLKASSPSAAAVPMTAGGRPTPLPSTQASLPANQGSGGSTSKAAVQSARGSGISPSSHQQQEGGVSPRPPRAPLTLGPQQGGGGGGAAGASDRGTVDRVAAQHAQQRHGRSGDKGLGMALTAGIAMALSAVQEAAVSACTDGGRQGTGSKRRRELDGDREMSVGGPGRSVRSQSPSSPPRTKKTPECRLKRAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVV1</accession>
    <name>A0ABQ5RVV1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="221" mass="23949" checksum="C126AE2B7B413B73" modified="2025-10-08" version="1" fragment="single">FTMADFKRMREEQRNLVASALQDMVSYVRSQIQAVGQAVQANLVAAQAAVAKPTRQEARQQRFEAPGSQAWKTKSLALARLDAEQRQRNLHDAVHAAHLLLRFITLADCLMLGQLLELARLAVLLCVRQLEIPRKAGLYIVLGTIEDPAAAAATASVRTPGLPTWGRAGTLATVSFAASTARRRGSNGMPLSTSISQWSYGGDGTSPRIRPSDDTDDDAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWE2</accession>
    <name>A0ABQ5RWE2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF247">
        <property type="entry name" value="GH16156P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="442" mass="46650" checksum="57B878D192B7BBF3" modified="2025-10-08" version="1">MEGWEVTDSGARILGGGLPKLQRLILGDLTELSRTGYSSLAQLRGLQCFTATGVRGNNLTLEPLTALTGLTGLRMELALGFRDEGLGPLACLPALRTLGWWHAEAHVSTGLRPGSVSPWLRELSLCVELDMRALSMLAATCKGLAMMQVQGIAIPAPEEDPAFTAAAVAAGVAVITPANAQAVAIGSGAAAARTGTVVPIMPSLRTLIIGELHIPPPDEPQPVVRLRALFPALENVLVFQVLHRGMLLLEGLNRLKRLVFNCIGLRDELLGLLPPLPSLKGLQLECCSQLTGAAVGALRAVPGVEHLLLIDTPAVTDTFLKEAVVHLRGIRTLILSRLPNVRDEGLQALAVLAGSLENLELRFMPEVTPAGLQVVLQLPRVTLVAVSECVGIGKDACRDLGAALVAAGRDVIVEHETTVLVSVSLADEFGASEEAEDLGPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWS9</accession>
    <name>A0ABQ5RWS9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 58 central coiled coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004401</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049270">
        <property type="entry name" value="CFAP58_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083:SF0">
        <property type="entry name" value="CILIA AND FLAGELLA-ASSOCIATED PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083">
        <property type="entry name" value="CILIA AND FLAGELLA-ASSOCIATED PROTEIN 58-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21771">
        <property type="entry name" value="CFAP58_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cilia- and flagella-associated protein 58 central coiled coil" evidence="4">
        <location>
            <begin position="371"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="832"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="109"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="648"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="832"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="868" mass="102073" checksum="5A4B7D1C9B94DC1A" modified="2025-10-08" version="1">MAEDGGPVKPSEELENRAYEALERDFQEVLQELVGDKSLERFRIEYEKLHRALKKSHESEKRLIKKCRELNAEIVANAAKVQTALKLNEEDQQTITALKKEIEKAWKMVDASHEKEAKAKETIQQLKIEIANLTRLVEQGAGLAIGEETTLNELLKQKEELVRERDAQVDQIMALRSELMETQEKLRGSEAEKLQLEMDIQQLKDAVAEKKAEAEREIRKKERMEKEMKELRQQLEVRSSEIKQKQLQVTSTEEQVAKLEQMLREAKLATEKVQKEYNLLNEKMQKLHHDLEEQIHTNTQLLAENSAKQVELKVKEEEIAAIKQEASRVNKLREQTLKKTKQLEEQRAEVERERDQLRGELTQLERDLETKQKEVDTEKKKLEELMRERDVLTKLRSQAENATQKQIDLIKINENTKRNLEQEIQGYKTEAQKQSKLIYQLEKEREKYSIEASEASAKYMQALEEVKLREMAIIDLQKRIMEGEAKLKQQQNLYEAVRADRNLYSKNLIEAQDEIQEMKRKFKIMQHQIEQLKEEITGKDLYLLKEHFDHQKVIKEKDLLRAELDKSKAQIKEADAAIGSQKAEIDKLNHIINEADQERIRQKKEYDIVVNERDILGTQLVRRNDELALLYEKIKIQQSTLSKGQIQYRDRLNEIRVLKVKLADLKRELHILKSSVANIDVLKREVHQLGRELLQERTKVKALSEELENPLNVHRWRKLEGSDPGTYEMIQKIQTLQKRLIAKTEEVVEKDLLIQEKEKLYMELKNILARQPGPEVAEQLSIYQANLREKTKQMKAMASELNMYQAQVNEYKYEIERLVRELNEMKRKYFEGKRREQMERERSLKPPAPAPPNANQTRFTGGGFSLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWU8</accession>
    <name>A0ABQ5RWU8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004421</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00299">
        <property type="entry name" value="GST_C_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03046">
        <property type="entry name" value="GST_N_GTT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="30"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="97"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="26702" checksum="2A8F01B20005A5CA" modified="2025-10-08" version="1">MLDFQMPQIGTVRRPQSMNRGGARCGVISKAQIKLYTNPGSRGKITEWYLAELNVPHELVSVDMRRGEHKTPAFLAINPFGKVPAMTDGDLPLFESGAILLHLANKYGKLSDNELGTAAQWTLFANSTLSEAFFNDRQRATQMPVMLRTLNELLSKKPYLTGEDFSVGDIAVGSYLLYLPLFFPDLDLTQYPEVWSYMQRISERPMCPALYKEAMAGALEAAAQRQGSAGGVMSKLFGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXS3</accession>
    <name>A0ABQ5RXS3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004600</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF139">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="366"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="455"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="948"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="465"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="948"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="970" mass="103332" checksum="C628A8971C545C31" modified="2025-10-08" version="1">MMKILNLGSLSWTFFGNAFQDNLAQRVERDLAPQTTLATLDLRPKYCRGKEQGKDGHLQAEAASLASPSPSCTEAVMSDFREMATLCSHQQSLHGHVDAPSPTVALAAAPATSLTAADTAGQVPAMEPARLSCTQSGIGAHGKVAVTAAGVPGAATVFAAHVLSPLGEAAVSGPSPDGVLSPVSPPSSRRSSLSALAPLSSTLGKTVYSSGDHVSVSVSSSGVTDPNVEIASGADCAASADGPVPGGPGSCQASSGRAPSQQPRVWVVNQIQVQSSNLTAAGVEVVAGAGDMAAAAISVVPQGPNVLQRRPATLRLRCIVRDPLTSCMLATAWGHVDDPELPLSRPRGRPIPGGIGGCEQPGHLLFVEEQVLGRGAFGFVTLVTDAVTERSYALKRMRRSAVSPRHVMQEQQALRLLIRDQSALTRPVADLPSDQEQQEGAIVCSRCGSIMGPQPLTAEGSSPVASSCTSSRMSAASGTPHTGGGRCAAAAIAAAGGSRPQQPCQCRFCTSAVAAAGCARNQGGSGTTASTTMGPARAAWLAKQQARGLAGLQQQKKYRSTPQQPSTQCPRQQYQQQLQRHQVVHRCCCRRLRGRSFCVRQLGTFQDEQYLYLLMEYCPGGDLDRLVRTVARKTLVPRSSWLAQVLAGPAVAWRGMPEAAVRFYAAGILLALQELHSHFIVYRDLKPGNVLIDSSGYPRLADFGMAKVLDGPTGRAASACGTLDYMAPEIVKLECDQLAREGKGQGLENWVDSGLLRRAAAGGGPAGGAVAGRREEAGLGRRRRQDNLQGTYGLGVDWWSFGAVLYVLFTGCKPFCPPEAEEAGEDPTRVLVRIINPWYEVPLPVYLSPNAKDLLRRLLVRQPKWRLGCGGRGAEEIREHPFFMGFDWDTFEERKLAPPYPFQAVSLPHDQEAESVAHFFGFYSRSLQDNPAEPTAALQKQKQHHIALGEHGPDPMRRQQQPFPNWLQDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXY2</accession>
    <name>A0ABQ5RXY2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">type II protein arginine methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004435</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NDUFAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF5">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="52"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="364"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="560"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="631"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="671"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="569"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="645"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="671"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="694"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="714"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="727"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="741" mass="80292" checksum="2ECAABA846EC0D3E" modified="2025-10-08" version="1">MNRLLNVFQNAAIAHAINHGIQSSLRCSTYLSSFDFVAAFIPGSWFSTAAATPGDDLQPGTTSGAAPSDANPSSLGPTQHPAPSGNPSATSGSSPSPASPASSLAGAQLHPQGHDELLPVVPVFEDEALAAQLQQPQPVQLLQVVPSPEGAPRDKLLVRDFIQNSLYHPTLGYFNAPTPPVGSVGGPINYWRIYCRDEFNILVSKKYQELETSFLTPAELFSPWYGACIARHIVEHRRHHLAAEGQPLLIAEVGGGNGTLARDILDWLRDNRPQDYHQTSYTCLEISTSLAARQYDKVVRQGGHGGRFHLRRGSGLDPATWGSERRWEHTFVLMMEVLDNLPHDRVFRRRASDPWQQTAVRLAQTDASPAPAPASAPAPSSSQPPLPATPPPGRGSSSSSSSSSMERGPWQLVGEPLSDPLLRRTLSAVYRAPTREQQLDDRFYRVLDFILARDSQPASRDEVLWLPTACAAFLELLHDLRPNHSLIAADFDKLPDVRLPGRNAPLVAQKVGGRNIDHDTLLIPWGTADIFFPTDFDALCALYRSAAHHVWGREGVAITSAVPPPPAPDTDTASSSSGGSDEGGNVGDVNAEHFRQGAVIKRYREMFNTATICAFNPMVDDFVNARFFFGDSKDRYPPGQERPAPQSSKPRIPQAPSSLVTAIKAHIPAAPAAKVRARLQQQGQPLQHQHHHLPQQQQQPEPVLGGRRQRGGLDRSSNSSNTSGTNTGSGSGSRGRGGSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY41</accession>
    <name>A0ABQ5RY41_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chlorophyllase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004988</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017395">
        <property type="entry name" value="Chlorophyllase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428:SF14">
        <property type="entry name" value="CARBOXYLESTERASE TYPE B DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33428">
        <property type="entry name" value="CHLOROPHYLLASE-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07224">
        <property type="entry name" value="Chlorophyllase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046730586" description="Chlorophyllase" evidence="1">
        <location>
            <begin position="29"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="39519" checksum="4F816217A083D8A9" modified="2025-10-08" version="1">MTPLRRSHLHSAAFLLLCAALTAPAVFAQDFWLNGQYSVVTYKPSRPSSPYGWKVDVTYPTGNSGSFPLIFMFNGMECQASWYKDIVDHVASWGYVIVQYTTGPSYPFMFGERKEVQYLTPLLQWLEKDAPVQEPELAGKIDFSRKAVMGHSRGGKLASLHYANSTNNFTTAVLFDPVDCSFFAQRDMHPSAIAALAGVDAACDLNNPAPHGPSSLSAAIIGAGKTGGCNPEGNNADAFFETLSSQSLYIKVKEAGHMTFARPADWSLFYWILDQVTCGTCSCSNITSEEVIYEARTMAVAWLESKFWPSQKTTARLARYKERLDNLTSKGVDTIRWPFGGVSASNPLGAGVEVPADAAAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY96</accession>
    <name>A0ABQ5RY96_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005272</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124:SF14">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_25, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="89"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="347"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="383" mass="41458" checksum="515E779D83E98D17" modified="2025-10-08" version="1" fragment="single">MGSSLSSCIQPGPVFALFQAVRHGDLRVIKFRLDMDPELLAYRSLGSGDTVWHLAAEGGSEEVLGALEAHGGQLRSGLYPEVIKLQNDRGQTPLMHAAQAGQAAAVRWLLSKGADPWAQDRCGKRSALHYAAMRGRLDCITALLDNADSTQELCRYIEAQSISGLTPLHYAVAMRQPDAVLLLLERGADLRAVNSVPFASDLVNLPLRSTPLHVAATMRELPCALVLLRYYHIHLMGPNFPDPRRRVDALGRTPYRVAALRINSGLLVELLHPASDLRALVSREGEPGEGPDMAIGVPRLAALMASVVRQQLLADVEDAEKVTLDFRRRNPCCSSPIRMAESQFQMLPASSTQPTVSPSASAPPGPAAATRIHARWEKASSGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYH1</accession>
    <name>A0ABQ5RYH1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="841"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1011"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1072"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1116"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1260"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="859"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1029"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1087"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1118"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1140"/>
            <end position="1151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1317" mass="134455" checksum="3915F63F7A9B0E9E" modified="2025-10-08" version="1">MELILDEAESAFLQAILTGTEAAPQVGVAGGRGVASMQCSSDIATDALSSGTKRTQGADAATLRVDGSGVPTSLALHSLQPHDKPSSCSNSHTISRSPFPSGFAFMVQRGRPWQQDLLPGRVAGLPSSIVSVTSGQQLHQDRLLSQTLGQAPLLQQQQEINSHAAGPHIHQGALSAIHAAMATAATRGPGDDAGLYPTSPTTCHQSGVTQLQPHGGTPAPDPLAATAHPPRTSASSVLALPNSDSAISAVSESSALAKKAAAATESVSKLHSHRRWSSANLASASRGGVSAGSNSSRSFSSAAAIPCASTPGTAAASVMGGSRSASAGSTVAITTTAVTLTVPSSRRAHGLLQRIQNLEAQLTELLLADSDGDEAGTNPIGSAAGPTTSASAAICAARCLRMPAEAASVPTISVPPAPFLRPADLNHNVHRHQHGRPTCLRKGQQHPHQTVGRQAASDSTGSPQAAYSSLHQSLVFSVTEQLCRDYNSSGVALMEAGLDEAAREQLDKALLLAGPKGPLEGRTEKQRELQIVAHNNLACYYRKLGQPALALQHLQHTALLESAAVMDTTEGCAEGRPASSAHCVPGCDRNGGDGGSDAVETGDGAGGNGGDVLLAGLGMGRGPTAATLLNLAACSSSLGQHTQALSYATRALQSAAGELQIPLPHLEAALLQGLPLVQLWELLPQLRRACQGATTQLAMAYYNRAVEQEHLCLWQAALESYKLAVDVASRFVGRGDDLTLTIQQSSEAFKVRYRKLAKLGKVRNTRVGNQGVLGTGALGGGHCPGRSPSLSTGAAATTGPGPGCAALPLPLPRPHPHLRPLLRCATAVNTPGTGTAHAVAGAAEPQAPKSAQGLPSIVPTRSSSTPSGGSASPYEPAATQTPSPMNVISPVSWAKNPPAVSEAAPAQVPRRALSAGRDRPQRRLAAAALLPARGPPVTSAGWVQSQVGRGNSAHLGADAAVAVAIRMVADSLLQGKDSYADPEEEEEEPLHRGDVSAAMAVLGDPLMAAQRNSAWSPPLPPKPKSEAFGEGGRVAGGGGGHGSRSATQLAGPVTGILCGGLSGAWVPATRGGCERLSRGSNPDIAGQPPPESPRAPPPAPAPGMIMLANVASLAATGAHTQQNASAPTSMSVETAPHVPLSNNPSKTQWSAGQGAAPKGGESLAAVAAEAIAAAEELEMICAAGEDGLPLGMHFGQGQEQAPKQGRDLQTEYEQRRVVCGQVQVPEGVNEHQEKRANGQCPSRMEVKPVSAQLLVQSPPAGMSDGLGRSGAGDVCRKSGGSSPSRLAPHYAASTGNMLVPKRDGVAVYPDLQFITNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYQ3</accession>
    <name>A0ABQ5RYQ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM3</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_004766</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008046">
        <property type="entry name" value="Mcm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF46">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01659">
        <property type="entry name" value="MCMPROTEIN3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="311"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="685"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="737"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="701"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="746"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI62182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="907" mass="98638" checksum="6A86749109F279C8" modified="2025-10-08" version="1">MEDYSEVRVQLTRRFADFFEYDFITPGQGSWSYARALEDLYEPVEGDGKAYCIKSRRLMVAEYHLRKYDESLLLQLLQKPLECLPSFEDALQNFVKSGVDPVLLRLLEETGNLHLSIGLKGDFGRHEVSPRQLTSAFLNQLVCVFGIVTKCSLVRPKLLTSVHYSEGTKEFVTQQYRDVTSLRGAPTGATYPQYDSAGNPLTTEFGLCKYIDNQTMHVQELPETAPPGQLPYSTEIILEGDLVDLAKPGDRVSIVGLYKPLAGKQAGGNINAVYKAVLVGVSVHKLSKESQIKVTMADAREIKRLAQRPRVLDLLGSSLAPSIYGHNIIKKGLALMLFGGLEKTLENGTHLRGDINALLVGDPGVAKSQLLRAVMNIAPHAVSTTGRGSSGVGLTAAVTFDGETGEKRLEAGAMVLADRGVVCIDEFDKMSDQDRVAIHEVMEQQTVTIAKAGIHSSLNARCSVLAAANPLYGSYDRHISVTRNVNLPDSLLSRFDMLFVVLDSMNDERDRQLALHVLRQHQYRPPGDDGRGATMQETIHDRRLSLDQDPEDPKIWLKIDTRLHGAAADSDQQVLTTEFLRKYLIFAKRKYARAAAQAAAKGGDAVAPSLAMEESAVKRIVDYYVSLRALPPSQRNFPVTPRCLETIIRLATAHCKVRLGDVIGRMDVAVACALMDHVMRREIVGEDDHKANGGRGRSNDDGDGGDDDGDGGGAAHDAEMEDATVADVAAQGTGAYGVAASAKPAAETRTRNANKRAREDSDADAVAVGETEASRVRRRAPRKGGLASEVVTTLAAAGVQTVPDGHEEAMAGSSGSETFATVESLTTEAKTSIVAEIGAMLEERGRDELVEVAELKRILNAKGLSISLLALSAYVKWISLNHDLELAKGNYLPDVAYDEDSNMFSFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZG6</accession>
    <name>A0ABQ5RZG6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005547</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056396">
        <property type="entry name" value="HEAT_SCC3-SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF0">
        <property type="entry name" value="LD34181P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24571">
        <property type="entry name" value="HEAT_SCC3-SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08514">
        <property type="entry name" value="STAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="2">
        <location>
            <begin position="360"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1053"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1339"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1485"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1731"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1056"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1383"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1524"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1538"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1568"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1637"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1655"/>
            <end position="1664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1681"/>
            <end position="1696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1778" mass="188809" checksum="85042722ADC2F249" modified="2025-10-08" version="1">MAESRRRSSRILAAENKTPAKAQKPMDSSDDEPRGNGSDDGGELTADDMQKGGTLRKGNAKSPEMRKEPSPVQRAGQRGGPGAAAHKKRKVPPNPAQGEDEPVQEDENAAGAPATAGPGPSSMAAAASAKPKSAPKRGKTAPLASLHNLTLWDIITKHPASVERAAKEWVDRYCQDKLEATSELMSMIVLAGGCNSGVSVEDLDSGEMDDVIKRLVDTIVREGGMEPFRDKKLRNLRPAFEAFWRVLIADLHAAGHLLDDYVCDRLANLLIGMSSAKVRGYRHAGTLTAGLLMTGWVCAQQQLAEAISTARHQEEAAQKQKTSGADKKRMVQSLQRQAEAAQSQARQLKSLLETTFTAVFAVRFRDVGPEIRAVVVDLVGRWIGLLPATFMVDQYLKYVAWALSDRDAGVRAVAISRLLELFGSSPTARSMAPGTTRVEPPAHLPLLHGFIERFTVRFKELPYDIDEEVAILGVQLLTRLVAVGAITDAQLPASDCYSLLIDNPPAIRRAASGLAGQLLQEDATKLESSYAQRAKEAADAMVAAAANTPRAGGTRRSGRSKVAPAPAAAATASDAAVATTLLSNPEQRKKVAMLGALLGMMSLLRTGQSLTQFTTGTNATSTALMDQVLVDDIVDALYDRLPVLRSWRLIVDCMSDDLLAQLWGPTGLAHLAELLAAAVKRARSGASVTEKGGVNALRQGPRSRAMIADGGALMEASQVLINALPRLLRRHQADQNVVCPLISLIRDLKLELFSLSGDDAGWRALLTLVGEQLAMRGTVAELFTQCAETLTFAASSGPPALQPSAGMVLRDVCEQLAGGLSTAATAVRVMDEEDLVNAVGELAAGDKGADVEELLSLRTALLRAHAVLIRGGAQLAADPRVHDAVDELLADCGSGRFLGPELTGLMAASQLVVLLNNVHQMNTDPQAAAALVPGVPGPSGSNAAPVLPSMLKLQQSRDALVAHMVALHAAATAHGDAMLEPDETGRTEDARCNGAGNVAVLQDMAFRVLSDVALVFGSKAWKGTAMEPVCQLAFSNEVADLMWRHCSETLRREDTREDVDEEDNEEDEEGMDDDMTTGPHGFPAKHAGRTAAVANKMVALGCLGRLLAHGVFSDYHRTMAINLVAEFCNHGTEVADLIRDICRELAASRPHGEMPEIYTQALRQSWTAVTVAGDVGEDEEHEALQRFAELAKHIASMYAGHGTSRSELLFILQDLTAKALVDAPTNLAILAWGAASFVPKLAPADAATLVSELESRLRGEGDAAAASGAADLVHDRDDPDWAPLYHYLSLLKGKASKGRVAPRALKGASATTPKAARNRKITFVLSPKDHGNEQDGVAEAAAGKLPPGESQGRKRGRPAADTTAPEDGAWLRKAVGQVPTNVQEDEEDDEEETVKPASRQQPTCRQVANTRDMQAANVADVTPDEGNGHARAKVAAGIGAGLRAVDVDAGPLDDLAHTQSQSLSRGTFAGTSKQVSLRAISDIGAEADEARFEESDEEDGRAARGGEISDLQAITEISVKTASDDDEVEADGAEQDAAEAHADREAVDGEDEGNPVVLPTEEELPSLARRTPAPAIRPVAVASAARAAPSVSRFSLTARSDDIGSRATGEASLDRTKNGNGRESGEGEDEAEERQCAQVSRDTSGGVSLMASESHGTEVRKGGVDEPADEATTVGDGKDGEGEEDNGECEGEENEEQVGKLEAHAQRTFSTRKRRLSYADLVDDFGIEEADVDNGSGQLLPLPSISAGTQEASMRADSTVDTQGQIEPPKRRVKRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZH9</accession>
    <name>A0ABQ5RZH9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005887</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="384"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="459"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="660"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="715"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="798"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="484"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="547"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="574"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="715"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="805"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="848"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="987" mass="104306" checksum="E619CD4F08BD44B2" modified="2025-10-08" version="1" fragment="single">MQFLRRLRNASTAAPAMEDGPQPSAAAEDGDGNNITTTSLPVVNTVDNKDPSTDDTSGQDQGTRTTPSSFGGQPPPPSIHANATSSEPDPVAASDVAGDADVGGAQTLSVESQGPSSTTVPPAPARSSSAITAASTPVLAPRTQQTTSYLQDPQNPNQPSVVKPSVVVKSMTVTNEPNRTDPRAQQASGDLGHGDRSEPRITTAATVSNQNTPSPPLSTIADATDPSVASGGNRQTAAVAKPVAKSTAAVAADAVEPVAALVASSPRFLHRQLKEERLQALAEAHKEKLRRLAAERHQIAQQQSLYGASGPMTAAAVAAAAGHGPPPLWRPASDEDGREVRASARQQMIEQLQTEQVAALAALKALKKGKTLDEARRIGNMQYEKQYQQQQQQRDQANVAKKASWRRDATKSHQLTAAAKPAIAAAATAATAAAAAAVPPALLTGAGLPRSSSVLLRLREREREEEEAANAGERRDPSRRVPAGHGGGGGGGGGGGSNTDLGPPTPTRGSSPTIQGRGLVDRRRAESLDRGVRLGGIGGSTGWPNTSPSLTSALSYSSMSSSPSGLRSNALRLARREEDDLESGRGELQTARGTGAAGGPEAQVCEPPTTARPLQPANDQQIQRPPTVDQLPPSYIEELKEQVKALQVVVEQHITTQQQRQHQPQQLAQDQQNQQSQQQLHDQDQAYRQLQQQQLLPGYAVAGQMPPPFWPMPMYAMPPPPPPQQQQPAPPPPPPPPPPPKPVDPILAGLPPEVAADPDLRAAHNRHTRDMALLRMEIDRARTAAELEQIRAVLQQIRGTSESTPSQKHQQDPQQQQEQQQQDELKQQQAAEAAAAAAAAAASAGFGAPPPFPPPEEAWPEQPWARAGGGEREGMQLVPGGEVALDREEGNSTLGEGLPYTNQEDLSEVMIRNSPPPPMSRLGFMPESHVLEVRLFMAGPFHKRGTYRVRAVLYDGFQPMEAAPGVMVGASSLHYEPTSEDDMGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZN1</accession>
    <name>A0ABQ5RZN1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005989</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47661">
        <property type="entry name" value="PHOSPHOGLUCAN PHOSPHATASE LSF1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47661:SF2">
        <property type="entry name" value="PHOSPHOGLUCAN PHOSPHATASE LSF1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="2">
        <location>
            <begin position="762"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="620"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="450"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="621"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="888" mass="90310" checksum="4293A162F140009C" modified="2025-10-08" version="1">MLQRSSRPVPAQVASTSGCRPSARAWPWAVGIAGEKLHLLHNQQWTMLPGLKSAPSKPCPPTSAARSNSAVFRHPGELDLNLREYMVVLEKPVGLTLAPDPKTGQVVIQDIKRNSPAHKCGLIQVGDVVKCCSAVFGDDMWAAVDIRRVRWAINSRMGDVKLLLERGRRSEGGLTAWYCGGKAGVATREMDGMQLPYAFNGTTAPEDASSLAELPGAGRTLQEVFHRNIITQPPPSRRASIAAAARTAAVQRLRRELSEVRNMRALDLEAIRKRSGAPDGFMLPPTAQSVGARSGSGGAAAAVSSPSQRAPSGGGSGSGPPSPPPPVVDVVAPPPPSSASVGAGNGSANGSDGSGNSSGGAGGGGGGGGLLSGLKLGGDSPAGGAAVITGMSSDTSTKKVVSFLPWLLVACGRLEHRDMATIASSRSLGTILELSITRSQQQPPQPPGSGPTATAAATAGSSSSSGGKSGGFLVVRGSDLMPLASDVGRVSYLALLAAATAAANPSGGGAPSPPPPPPIPVGEEPPPPPPGSAAATAAMINGADVVAADGSSLVTTSLTDAAAASGGGAVSEGTGSNSKSGGGGGGGGGLGSSTMAFLNRSIFLNLRPSMPFASTTVPKIASPGSASATSASSASSPLSPSASGATAVRPSFSAPPSSRYDPFRVRHLLNAAASLQRLAFRRGGKLHRARRDACAVLLHCEAGMEADVAVLLAGWLHWYGRLPLQEAILAAETAMGATVDQALLEAGTLALLQGARDRSSRVVLTWKYGGLSAYVAGDVVASWSARVPMVRCRSPLGCKGDTHPGHFFLEVQGLRPGVYYYKYIVDGTWAVDSYSSKVLDAAGNWNNVLVVPEPPVVLTSREGLALARWQAARLALEAKMGVIGTPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZV9</accession>
    <name>A0ABQ5RZV9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005382</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037">
        <property type="entry name" value="FORMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037:SF24">
        <property type="entry name" value="POLYCOMB PROTEIN PCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045591690" description="Peptidase M11 gametolysin domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="4">
        <location>
            <begin position="130"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="514"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="550"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="642"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="672" mass="73292" checksum="6B48AB144941467F" modified="2025-10-08" version="1">MALRALLRLGLAAFLLHVSLLVATAQVLSTTSPGSLRGIIASVDLHGEEYMEPAYYFNHPDGVIYRLVFCTNISEDDIPVGVSLTVAYDKIVDGVIYSCAVPTAAAAPQPSRGRMMLGEAITTPLEPRILVYITTMCDYTDQAATQQNVKDLFFTGKGIKRNRTMADYYSTCSYGQVDLLPENVVVIGPVYVPCNGSLGSNSPFSTGPNFNTSSCNDNMAKWQYWLDTNWAAKQGINPADFHHRVIILPKSFSSRVNGCGGFAGAATPGRWSFGKTPLNSWGSGLVWWSGDSVALQDIEILLHEIGHTYGMAHASVDGGCELVDQCDNTCTMGAMGGQNIRCFNAPHNWQIGWSKPYRQYDESGLAYGKVVMVRMPSQAATVNNFVMLSVPKMPQNQRLFISVRLNSYPYDLPYQPIMDGTAFLLLHTYDGNDTDTYRRTVLMRQINVGSIWRDNVTGLAISFDSWSPTAGAGLRICRTNFVKEALSEGNCNDGLDNDCDFQTDASDADCQATEEIFGGNPDPNPALPGRSPQAGASRPSGSNPSGTSPPRSPPPPPIRVRIYSPPPSPLPPPPPVPSPPPPPTPRPPRPPFPRPPRPPRPPPRPASPPRPPKPPSPPPRPRPPLPKRAATSRLDFMDFNASPPPPPPPPPPPPPPPDAWEREYPFRRRRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZY0</accession>
    <name>A0ABQ5RZY0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005967</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="851"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="922"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1034"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="862"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="969"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1039"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1269" mass="131117" checksum="CABD9C10DF61067F" modified="2025-10-08" version="1">MRVGHKSVRAIWCARVLPDTGQLGVRGIGTTNSSELFTCGGAPRDGHPARHSDCGGSGNGVRSFIADEMATQPQSNVREENKSGARNAGAALVAPPTLDWGLTSVATTPFHEATAAATRDGAVILAGSTSVGAEAKAITTPVAAAGGLTLRMIHRMQWWSSFGLISPSVSVLASPLLLPLLLPGPQPPSSGATRSMSAVAPTITQELRSCISLLQIEDVVSRYKSRMRPIHHAEAVVQLGLLTYGSRSKQRVAARRLAAECTAAALASASGGGSGGALAAVGLDVLVVLVRGWGWVRKPPPELLMRMLEALVPGSSIRTGGGEHRHTQNTSGVRQGYGRLHTATLCDAAALVEPLAALEALTPPLLEVLINIAVRRAGNEASATVAAAAVAYGRAGVTEPRVAQAFLEATATRLRGGAAAASAGVGHIAEALNLCAKAAWYDPRVFTEAADALVARGVGGLTVRQTWSVLRAFAGTRHEHPLLKDALVAHAKALFTESKSRFEINFQGRDDPTGNHLGYVGRKAGDAEPLAAAPAIDFAGKDVVDFSAATVAAATTDVGVDVDIDVFNGFSPPQRQLSEERELTRQLSTLAGLVHGFSTMQVYDRELYERLAAGATTLLCRAMVIHLGESQPRALAAALTALLSGFAGAGIYPVRLLEVMTAQQQGWMRSISVEQAFSLTCALAQLRYRDERLLDRLDAAAAAFLEKTVATAAAAAGLLSPASSGSGSAAPATATTTAAAAAATNPDMSWLPSFLDAACRRLSYPCPRTVATLAAAMATWDLDEYGNTVLRGLSKGRKEKRERGAARYSPVRRTDGLETLSRLQLLLLLVIEQSRRGAVTAVTATAAAAAGTVGGRGKDRGSGSGSGSGSGGDPVLTPHWVCELAGSLANSGARLLKDEEQDLAWLLAKSIEMAVELEGGGAAAPAAAAESDESSTPDREPVAGGLRNESQTAEEAAAAAAGLWPPKRQQQRQRRRRQGLTPAQAAAALDDTAACHLFRAVIASQYFPDQRGEDEETKHLQEVEEEVESGFGYLRGEDAAGGGPGRGGGGGGSEDDDDADLVGRRGLEAALHRALTAPVAAAVLARAASAWHAAGLGTAAPRNTAQHGVITTAATATETKAAAVVAPPPPPPRRLWRGGGDAAHSPMLSELLHSLGLPVFAHWETADGFFRVSRGVVLPGGRHVAVEVLPDAAFAANTAASASAAAAAAVSNGPSGPYPVLIGDAAFRARCLAARGWRLVALPEGEWAAAAVAGPAACMVLMRRHSGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0B4</accession>
    <name>A0ABQ5S0B4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="480" mass="50521" checksum="CEC16FF064534273" modified="2025-10-08" version="1">MGCGASKGTETIVLQAAPNIGGSQPEFTPETKPRPSIKPEDLSKSKESRDSPAKRVDAIVKDSVSDFNDDTETTFVRPLTAEDQSSVQPLPEAVLPPPPVTPPAAAKKPLFQAADTSTLDDAPLEDGAQALYFPTQVSLNVGATGGGGGSYHAQQQQQQQPQRQPESQTSLQAFGTSLRPSAPELDPAPLSGGGATFPLAKPALLPVNSSRQLQQLQQQSSNRALSPIQIQGQPLTESGPGPMTSSSPAQPAASSSTADDPWSSLQSTYRIRENQVAQKSESTRTRAATSSREAGREVLVLGDDDDEFSGGGGVLNSTANSAMNRTSGFGGRPPMSLPPPQFGSGPKKGGWGSGAMAQAGELMIGEDDWLVNDRPPGMPSAGAMQLPPPPPALQRQQQQAVLSYNSRAKMVVEEEDRHAPVKLKGPAAAPKPKAADDVMCEDVEDVEALLEDEMERQGLSNKNLASKLAAYEAQFKDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0V3</accession>
    <name>A0ABQ5S0V3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006290</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex with RFC1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="36"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="328" mass="35337" checksum="01452099201F167D" modified="2025-10-08" version="1">MSTQPWVEKYRPKNVKEVAFQEEVVNTLTRALETANLPHLLFYGPPGTGKTSTALAIARQLYGPELMKARVMELNASDERGIHVVREKVKSFAAAAVGAPVPGYPCPPYKLLILDEADSMTQDAQNALRRTMETYSRVTRFIFICNYVSRIIEPLASRCAKFRFKPLQPVVMAGRIEHICEKEGVTLCPGALETLSAVHGGDLRRAITTLQSVARLKGGAVDKATLLDVSGRVPPDVISGLAASCKAPGAFAGLQQLVTNIVAEGFAAQQVLQQLQAELLSDRSTPGLKLAEALEALAAADFSLTVGADETIQLLNVVGQIHNAYVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S111</accession>
    <name>A0ABQ5S111_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="59255" checksum="9FCD29E2C14BB14A" modified="2025-10-08" version="1">MAEPPPLLQQGSRARRRALRLRPADVIWGICLGLCGLILLLYGWHLRSRNAFLAGSSSPLEVQEGVITDEHGATTAPQRQPQYSTAPLLVSYAFYEKDVIQRSNLEFFLQHGWVGPPAVVGFAKGGTGPPPPSPSPITWVFVVTGSLCSPCVGAFPIKTPIAADPLIGVTRAARIDGDPEDAAQGGGSSRSSGGGRSGASTANDVLAGPYGGPPAGPTLLLWREENVGMDLAAHNTSLEYLTRHAGGIRRYRYFLLINSSVKGPYYPAYLPTWWHWTHGFLERFQPLPEGDPRWTHHPTMQILPPGTPYGPMRRRQRPMGPAVVRAVGCSLVCLPEVDAGGPGPRLESWAVALDDVGMELVIRKGVFKVRTCKTCADKDDGIIVGGEYGITSVLLEGGHNVATLMSKYARDVDWRDERHWGCNDMVHPSRHGTYDGIAFHPYETIFVKSSWHVADPYTRRYSLWMTQHREGDAGTDGEWNEKMYRYAISTEAQMANLLEAAYNVEKVQETLAKHREQEQRILEEEQRQAGDRAASLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S158</accession>
    <name>A0ABQ5S158_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_005848</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05147">
        <property type="entry name" value="RIO1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="216"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="22"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="119"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="532"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="610"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="535"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="610"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="626"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="655"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="685"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="710"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI62991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="731" mass="79415" checksum="3270058E9DDD80C5" modified="2025-10-08" version="1">MAAILPDEIPFSLLQSRYAVDEDGDDASDCHQPKASPPLVDSVGAQELDTAVDPAANIVENDDDEGESNDGEYNVSEDEEEAGSEELAEALEWADMRDDLMNRGSIGMGYTSGLLRRPNAAGGAANRQAAFQPPSASMQRLDSHFHGGRLGANGRSAAAVLDDPLDAFDGVSARVSTSVANVVRTTALREGAARIASKDKSDRATVEQAIDPRTRMVLFKMLNRGLFSEINGCVSTGKEANVYHASGADGRDLAIKIYKTSILVFKDRDRYVSGDFRFRNGYCKSNPRKMVKMWAEKEMRNLIRLRAAGINSPQPLQLRLHVLVMEFIGEDGVAAPRLRDAGLPLQRLRSAYTEMLLVLRNLYQKCRLVHADLSEYNILYHKGELYIIDVSQAVDLDHPKALDFLREDCKHVNDYFRRSGVATLTVRETFEFVVDPAINDSNVDVALERLSEVAASRPTDVVPEDEVAAAVFHQAFIPRRLEEVVDFERDHERLQRGGKETEGIYYTTITGMKQDGSGARLLPAVLETEAKAATAGSSDAINPTETRSQSEPPREVSLSQKHHAKLAGAAHSCSTDGTSTIVKDEVVVSGVVRESLSLLSAPLVAVSETKVTTSDGESMNAGSESEAVEVEGDDGITAESGRPNRRIHQGAGGLDEGDGEEADEEESGTDDEGEEGEGSAGDGGVAERDKDAERAARKAHKKAVKEANREKRKSKVPKHVKKAKTKGAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1E0</accession>
    <name>A0ABQ5S1E0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006751</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF148">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="44114" checksum="0D76ECBE755F8FC1" modified="2025-10-08" version="1">MAQVADEFHKVIDQLEDGHELHDKDSEPDAHGQGGYRFDSPEHQQQLKEQRAQLWSWLKRVGSHMFKEGINLTKISLPVCLFEPRSFLERLTTNWEYNSLLLAAARCADPVDRLRFTIAFAVSGLCRQVSFHKPFNPILGETYQAIYPNGLEVYCEQISHHPPISSWELYEPSGAFVFYGNGNWVAGIKGNSVKGRQTGSNCVRFASDGATVVYELPGLQVKGVLWGVRVLKYSGQMVFKDTKNDLQAFIDVDPPAPSAGFLSGWFRAKKAPAHKPDQVVGRIMQGDTVLDTCHGSWLHYLEWDKGVSPAGAAAASAAAGANANRRLWDIRTSRLIPAVPLENPLPSDCRHRNDVVYLKAGDQIQSQEWKQTLEEQQRKDRKLRREGGCSFDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1I2</accession>
    <name>A0ABQ5S1I2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mg-protoporphyrin IX chelatase</fullName>
            <ecNumber evidence="9">6.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_006220</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>protoporphyrin IX + Mg(2+) + ATP + H2O = Mg-protoporphyrin IX + ADP + phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60492"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.6.1.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Redox regulation; active in reducing conditions, inactive in oxidizing conditions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Porphyrin-containing compound metabolism; chlorophyll biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The magnesium chelatase complex is a heterotrimer consisting of subunits CHLI, CHLD, AND CHLH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Plastid</location>
            <location evidence="9">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.6.1.1" evidence="9"/>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01451">
        <property type="entry name" value="vWA_Magnesium_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.80">
        <property type="entry name" value="Magnesium chelatase subunit I, C-Terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041702">
        <property type="entry name" value="BchD/ChlD_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011776">
        <property type="entry name" value="Mg_chelatase_ATPase-dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02031">
        <property type="entry name" value="BchD-ChlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43473">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLD, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43473:SF2">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLD, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="9" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 9" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="11">
        <location>
            <begin position="562"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="402"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="407"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="425"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI63313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="765" mass="82828" checksum="F21B35A44B978F6D" modified="2025-10-08" version="1">MKLCTEVAGLNALPASRRRNARKAFSGARVVQPACLAKPAKFNRQQRLVCNAVAELTSAELRAINVSEADTVGFDAEVSTRLARSYPLAAVVGQDNIKQALLLGAVDTGLGGIAIAGRRGTAKSIMARGLHALLPPIEVVEGSFCNADPEDPRSWEAGLKEKLGSGPVKPKLRSAPFVQIPLGVTEDRLVGTVDIEASMKEGRTVFQPGLLAEAHRGILYVDEINLLDDGIANLLLSILSDGVNVVEREGISISHPCRPLLIATYNPEEGALREHLLDRIAITLSADVPSTFDDRVKAIDAAIRFQDKPQDTIDETSELTDALRTSVILAREYLKEVNIAPEQVTYLVEEARRGGVQGHRAELYAVKVAKACAALEGRERVNKDDLRQAVQLVILPRATILDQPPQDEQPPPPPPPPPPPPPQDNMEEEDQEEKEEEKEEEDKENDNQDQPEIPQEFMFEAEGVIMDPSILVFAQQQQRAQGRSGRAKTLIFSDDRGRYIKPMLPKGDKVKRLAVDATLRAAAPYQKIRRQQAINEGKQQRKVYVDKPDMRSKKLARKAGALVIFVVDASGSMALNRMSAAKGACMRLLAESYTSRDQVCLIPFYGDRAEVLLPPSKSIAMARRRLDSLPCGGGSPLAHGLSTAVRVGMQASQAGEVGRVMMVLITDGRANVSLAKSNEDPEALKPDAPKPSADQLKDEVRDMAKKAAAAGINVLVIDTENKFVSTGFAEEISKAAQGKYYYLPNASDAAIAAAASTAMAAAKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2F2</accession>
    <name>A0ABQ5S2F2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_006641</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF2">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE GLC1MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046576385" description="Alpha-1,3-glucosyltransferase" evidence="11">
        <location>
            <begin position="18"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="517"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="595"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="621"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI63628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="662" mass="71612" checksum="BACF6F1120A611B5" modified="2025-10-08" version="1" fragment="single">MITNVGAFLQLAVIASAIKLLLVPTYRSTDFEVHRNWLAITHSLPISKWYIEETSEWTLDYPPFFAWFEWVLSQIARFVDPAMLVVENLEHASGRTVLFQRLSVTVTDVVLLTAAYALARDLSSDDCSSPLSGEGKSKIKKLQGPRLQAGGVAEEITVAPTTTSHVASSGRGHGNSSIHSDSTGSDMRRSIALFFLLSCHAGLLLVDHVHFQYNGVMIGLLLWSLYAARRGRHLLSGLLFAALLNLKHLFLYAAPVYFVYLLRHYCCEGTVLGPACAEAGSGARSPAGQGSGKGSSSERGGYGLGRVVVRLALLGAGVLAIFGVSFGPFIIMQQMPQVLGRLFPFGRGLLHAYWAANAWAPYAAADKLLSAAIPRVAPRLGLDLDKLGLQIQPGAANMAGGLVGVSRFSVLPQVSPAATALAVLLTLMPCLAALWAGDGRPGYIRRSIVRAVSYSFLSGFVFGYHVHEKAVLVALLPLVVDAVRTPSAAHRFLLLATAGNYGLLPLIFRPQEYGTKILVFLSYFVVSMVALGSIHPEMQLDSRGTGVAGDSAASHTLLSPSEMQNTTSPLNPSLKRRAARSPMKGAAVFGRMTKLYLAGFLAVELYVSIVHEAVPALRRRLPFVPLMLTSVYCSIGVLTVWVDMVTEWVREASMGWRKRQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S325</accession>
    <name>A0ABQ5S325_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-mannosidase</fullName>
            <ecNumber evidence="3">3.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_007153</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054723">
        <property type="entry name" value="Ams1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017:SF1">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22907">
        <property type="entry name" value="Ams1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="8">
        <location>
            <begin position="560"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="678"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="740"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="682"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI63999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1297" mass="140899" checksum="1897EA160D39B206" modified="2025-10-08" version="1" fragment="single">MAAAHGTTPPPLTAPGPSLPRPSPSPCQGQGHVRSKGDGGGGGSVWVPQHHRDITLGRLDVFLAAGQFSDRTIRADLWSQRCPDSVSLEVYSHPKDVPYPSYDEAIRMPYRPAKVGDTFGPSWTTHWFRIRARVPDSWTGLGPLMFRWDSGSEAMLYTDGATPRQGITDQRNEYQLLEKATGGQELLLYVEMAANGMFGNGPSDIQPPDENRYFTLKTAELAIPNVPVMALWYDMRALMGLARELPPGNATGEMALYTANKIINRYRQGNPDCVADCRTMAAEVLAVRDPGERMQVYAVGHCHIDTAWLWPFSETHRKTARSWSAQLRLAEKFPWHVFTASSAQQYEWLQQDYPGLFSEIQGAAARGNFVPVGGTWVEMDTNLPSGESLVRQFLYGQRYFEANFGSRCDVFWLPDTFGYSGQLPQIANGAGIRYFLTQKLSWNNINSFPHSTFLWAGLDGSSLLTHFPPANTYNAQADAHDILLTATGSKDKDRAPAAYMLYGNGDGGGGPTVDMCESLSRLAGCRGLASVSTVAPGEFFRRVEAASRDLLTWRGELYFELHRGTYTSHAANKALNRSCELLLREVEAAGALAAVLLPPDQYLYPRDEVDAIWKDVLLFQFHDVLPGSAIGRVYDYTAQRYPQIASILRALRDKALEALLAAAAPPAVDVVGGGATPTLFPSSSSRTSSTTISSRSGSSGSSGSSGSSTQQPLLLGEVVGAKPLPAEISTHEGQTVYGKYYRGSAESPGPAPPAPSPLAGLESDDGIGAGAGVVVESAVGWLFNSLAFRRREVVAVPMEMLPSGMVVRQLSDDGTAALVVVDAPPLSLTPLVLKDLLTGYGSSTAAQGFTAAASATAGGDPWDDVRASVLYETNRNGRVEYVMQNSLIRAVFDSAGRLVSLYDIVWRRELVPNSAAGNVFRLYEDIPLFWDAWDIEVYHLEDSSQAGQGQPPPKVTILEYGPVRCRLGVEAQLTQSSSMRQTISLTAVSARLDFHCEIVWAENRTALKVEFPWLLDAPAASYEVQYGAVERPTHTNTSWDWARFEVCAHKWADLSEPGYGVALLNDCKYGHATHGNVMRLTLLRSPKAPDRLTDMGEHVLRYALLPHALGWAAAGVAAQGWTFNAPLRVIKPPSQRRRKTSQAAAAAVAGPDGFPPARVAVTGGGYDPTHPMFCVTSAGPNPLLAAAPPGPLSPPPLILDTIKLPEPPLQRAPQTSLPRARPSQPAPAYAVVNNALTPTARRLEGETPAASALVLRMYEPHGARGVARIRWPSWLRVSHVKRCNLLEEDQEDDMEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3D7</accession>
    <name>A0ABQ5S3D7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA2/NAM7 helicase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_007636</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="7">
        <location>
            <begin position="1314"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="8">
        <location>
            <begin position="1788"/>
            <end position="1874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="703"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="761"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="841"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1213"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1259"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1564"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1622"/>
            <end position="1653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="703"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="761"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="844"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1260"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1564"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1631"/>
            <end position="1653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1968" mass="210336" checksum="0864AD9E783D7CFB" modified="2025-10-08" version="1">MPAASVTVLIPTAPAGGSEATIQLSVTDVASFVGLRQCGRYLRLTVARNAPELTTDTQDALNNYSVSIDGALAAAGRQLEVHVAEELERRAPDAPYVNPCRPKEACTPWLVFLDLLARAPNPSSGYLGCWAAEVVLRGRPAVDSVLSLSGRADYIVLLWRSGRPVLRIVECKASTEPQTKYIVQLVLYRMLLLRLLSEAAAANNHRECVCIGGLEWSVPGRRAVHGVTALEVECLLRTKLSGPAGHATDGDALPMEEVEAMTRQLSGMLMPGGRIAEALSVQTPLVDVPYELDGHCGACSRVAQCWIESAARGAVQLSGCSASEAQRLAKAGVTDIEELAALLPPSNGALPAPPRPGPTRPRRLEDVAADSDIAPGELHRLSLIASVRTRRWLGHALSLHNEPGWRQQWSTVQLLPGAPPSGLPSFAVRSLLVRVYLSVSVDVALRRVVGVAAHIAAPDLSSGSAEEKQTAPPSQAAQTGWDVAILMEGVTLEDSSSSAVANTDMELDDMEAQLLADFSDQLCEVIQQCVERQQQQAQQLMLHFYIHSHEELTELIRRCNTLEHLHGLRRSSDALTAHSLVNDKSCAKRFGRGSGSSYASASGSSIGAASGTGLGWLPHLLGLRADIGDFQEKAGMDASPEQAMVSVIEDEIRRYATAWHGAGRLAATSVAWGSDRTIFNWRAPQPLRHTRVAMDGGAAAADAIPGSEAAKRSSSSSRLEGDADGDKIDLRAAFAPGGYLNSLVRDSRDLRSCAEAVAISEQQWTQRSLKQQGQARQGYGIPPADPQPRRRRQPESTPAFASGNLVVAQLHDDYVQVRPDLAGSSIPPGFFRALWASSSGLQRGRRDETQSPQQPEHRGEQQQHSNETPSPGRCHGNSDAVEKQADIFQRGASSLRSLLLAQAHAVRWLEERLARLPDGGNPCLRKVPLVLPQPLGRSLRAFQLDVGNLPGGPRRAPQGPQAERAIMLARAALDTMWLNRGGEIRQFWQGLGLQAPAARAADNRNGTVVLDRLRRMNEGKVHWLLGRVAAPESCRNWEALTEATRLEEGSRVVVTAVPDPSVCQDPCEASGRYAHGVITSVADSGLGNGTGSVEAEQELAKPVAGHSDLWVECRLDKTWYPTNGLFLSGTPRVWPWTFAVGGAASGRVALDAAPNISAWVATSVIEILQEFGGSSAYLAERLWLSLPPADLAAGSSFMHQWLEAASEGTLVAREPETGPNTPLSEVQVPGDIQDRSPNRPCPRDTATPATLLRGHEMLTHPQSSTSSNSILSAHARGLGGGSAGSADSTGPSNVTRGRAGGSGDDLVQRLAPELDQDQKACIYAFLKHPESGSRVLLQGPPGTGKTETVAAALLVWLAERMHRGGPMGPSEQGGVVLLTGPTHAAVDNIMKRIGVRLRNSTLSGDVLRGRSVLVRLDSDKKALDLSLADLGVQSSLPNRSSWQKNTWYLVGGTLRYLAKAAKQLRTLVASAATPQAAKLMEASGRAAFASALLMDEASMVFAPQLLALGCLLVARGSLLVAGDHQQLSTITNYDFEGDQRPNLLRHRPYVSAYEYVRLMQADETVPRRGQRQEPPEGHLQWIGGPPPPVSTCALSYTHRLPYAVRALIQPLYSRDGIILRGRPPAERPAADNTNTTITGSSKNSSAVTTRNNRASRFAARPTVATGTASLAATSTLMAAPFPLQRMSGGGSFGGVLPEKANAGGVWRHLWQGPACEEGVFLLVHNETGSSRCNDLEVQLVASVLEARLGSTARLNSGTSGELATADGSTGLIASSISGSSGNDRTGDVSVAIITPHRSQRRALKALVEERGWGRNCQVDTVERMQGGEAEIVIFSATASDAATLERSEEFYSSVCRANVAFSRARQRLIVVASRAFLEQLPTSYSRYSGLMLWRQLRAICQDRGAYLGAENVQMWLNPQARAAAAAATGAAAAGGQLPGGDSADHSGGGQHELRATVTYRCDVYRTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3M9</accession>
    <name>A0ABQ5S3M9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007277</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Whirly family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013742">
        <property type="entry name" value="Whirly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31745">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN WHY2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31745:SF1">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN WHY2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08536">
        <property type="entry name" value="Whirly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="229" mass="24712" checksum="A7FC6DFD6DC7190C" modified="2025-10-08" version="1">MLCRKIVGQAAPTWGGVPMGRRDIPALIQSKSQQLPFPVPIWKPDYKPCSSAPRSITGSASERTYSDFHIYKTRAAMAVRLLPPIFTSENGYKILNRDGVMLLEFASANPGQQANGGPPPAGGINRTYNWTNKVSFALSPVELGNILAGDAIGTETGLVMFHDPAKTGKVGEPVKRLTLKQMKDGAISFSLSAGHDNISVPVTKGEFEVLKSLAQFAIPRLLGFDVVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3R2</accession>
    <name>A0ABQ5S3R2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_007867</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024862">
        <property type="entry name" value="TRPV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582:SF2">
        <property type="entry name" value="INACTIVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="5 13" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1020"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1059"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1086"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1126"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1193"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="472"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="979"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1309"/>
            <end position="1334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI64548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1334" mass="150851" checksum="F9E65143498663AA" modified="2025-10-08" version="1">MKEIDRHSCGEVICSALSEDGNNAIVVSKSLSNECTVLFWDTRMQYESKKQRSLEVPSADPLLDIVCDISWSAQLVAVAEKHGSLFVWNVETGRMQFKTTFNHGPRSCCRFSPCGLFILVSGLNFGELILLESTYGQEILRAYPPEGIRTIRTVMGCFFWVPSSEPRARAGPDETPLPQTPQNTGDVALSVMSDGGTRGRTPLGDYRGYQQRYIRFNRDEGRVEKPNTDDDPLPFTFPTRFGAVYSDGSIRVFDMEGTSYTKSCQFDMAVDKQHGEIDKITDVDVSGDGKLLFVITSKKIRNRDPAELRVYDIDSGKLIWNQKNPDFCKSRVIPSGDSGLVSYDISGGFQSWHMQGRVVWSGSGEIHLYDLSQRTVQLTINTEEHHTYGYGLYPQVHWESQKMITTSDFGEDNWVCHWNLSTGEMIQKYDRIHMKAITGAWLTADGSRCISVSRDRTAALFRLDNGNFIKRFKGHEDYVTFAALSPNEDILVTASDDETVRIWDVDKETQRHVIQYKDWIHNIIFLLSTNWIVIKDVLGQVIGFDTTGKSSPMIFAGDAVKKCNIMKLRPVSPSGAAKSGSALQLPLMITVQQNFRINVLHLHTLSVRQSISSKGESNCFGLPEIVVLSLAKTFPGLAVAPHKDNGETYLHRAVEHSNIEFLRNLLKTSHFAITSAPLPQDCKGVTPLDFALETKNLELVELFLSNELERPPHMRLAARKAFSQLSKDATEQLLKFLDAMGSEGRRKGKASWSRAYIAPGEDELAKELDWAQSIAGRYLYRIIEKKRDPDRVVRYKVNASAWWNKLMNFRTPKQQDLVSPFPFMIQLAAIKIAQVIHEFPYAVNTSSGLLKWWNGAPRERKERRNKIFDLQSPELDSQQVAQRILMQLPIANDEQIMSTPPPYTLLLAVRKWGLPWMKAKENVKPGEIGLPYMATATRNPADSPLGALVDNEALLSTDVGTAIIMYKWNAYAGGIFKWRAWQYIFFIALYITSTTLDLSWNPKVDPESMYGNNTRRGHVATRIVFQASVQLINIIYLGMEIKQLKHLGFGFYFKGPGSLWNWIELISGILVIMVMVLQIANVQEAYWLMSACTLLLGARLLKVLSVFMGTGIYVRVILRIITDIRHYLLIVFVVLMTYALSFRHIVVYYNQAANNIIPDNFTQRFNSFPNSMLSAYYFMVDSLNTETGNTAKYVWYLHVMLISFSFIVSIILLNLLISIMSGTYADVKQHAEMEWRLLIAKDVLEIDSSRSPAYFGGQFKRLGPMWIPLSEVWMKENASKLHKEARDADINDLKQQMLSLQQDMRLLMASNKGNGGVQPPGDKLTAVAGSSSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S470</accession>
    <name>A0ABQ5S470_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF924 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.320">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010323">
        <property type="entry name" value="DUF924"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06041">
        <property type="entry name" value="DUF924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="29195" checksum="5CDC6DE3D9B4BF80" modified="2025-10-08" version="1">MFARIRRLIRTNSRASGASLRKTLWLSETQTQRSRELSHNLRLMATNDVIPDRARAVLEYWFGPDYVTADDTFVPRDKMQIWFRGTPDVDQFITEHFGRDVQQVREGLYDSWREGSPLSLLAGIILMDQFSRNIYRGKSEAFALDSKALSWADFAAETGADRSLPAMLKYFAYMPYMHAEDLAAQERGVELFRAAAEATAAAGPAAAGAENSLRMALKYAEAHRDVVAKWGRFPHRNAVLGRPSTPEELSGLADGSIAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S513</accession>
    <name>A0ABQ5S513_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA 5'-3' helicase</fullName>
            <ecNumber evidence="17">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">VaNZ11_008408</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="17"/>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010643">
        <property type="entry name" value="HBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001945">
        <property type="entry name" value="RAD3/XPD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06777">
        <property type="entry name" value="HBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00852">
        <property type="entry name" value="XRODRMPGMNTD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="20">
        <location>
            <begin position="7"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="GLI64983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="782" mass="87881" checksum="945355CA214ACF2D" modified="2025-10-08" version="1">MRFNLEGLTVYFPYEYIYPEQYRYMLELKRALDARGHCLLEMPTGTGKTITLLSLITSYQLAHKEVGKLIYCTRTVPEMEKVLAELAELIEYRAKYLGRGPGFNEILALGLSSRKNLCIHPRVAEEGSRESVDAGCRRLTASWVRERAADRVSRGTTEGGGEEDGGSESAVGDIELCDYFEGHERAGVEALLPPGVYTLSDLRDFGRKRGWCPYFLARHMMAFANVVVYNYQYMIDPKVSQMVSRELEKECIVVFDEAHNIDNVCIEALSVNLRKQTLEAAGRNLGKLSNSIRRAREADAARLQTEYNRLVAGLVAQGALRGGEDWLANPALPADVVQETVPGNIRRAEHFMGFLHRFLAFLRDKMASPVITSQTPQVFLHELQERVQVDAKTLKFCYDRLSSLLKTLEITNTDEFTPIQLVADFATLVGTYAKGFAIIMEPYDERLPQVPDPVLQLSCLDASLAMKPVFSKFQSVVITSGTLSPIDLYPRILNFNPVAIQSFQMTLTRDCLCPVVVTRGADQMPMSTKFEMRGDPGVMRNYGRLLVDLSAVVPDGIVAFFVSYRYMDQIIAKWHDMGILQEIMQHKLIFIETQDVVETTLALDNFRRACDSGRGAVFLSVARGKVAEGIDFDRHYGRAVVMFGVPYQYTLSRILRARLEYLRETFQIKEADYLAFDAVRQAAQCVGRVIRSKADYGMMIFADKRYQRHDKRDKLPQWITRHLKEAHMNLSTDMMVHVAREFLRAMAQPYDRGAVGKSLLSEAALAALDAAGGLDGAGAAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S535</accession>
    <name>A0ABQ5S535_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007885</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="5">
        <location>
            <begin position="8"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="365" mass="38670" checksum="9B5FC8B0D79D915E" modified="2025-10-08" version="1">MANHEKPRVLIIGAGFAGATLATKINGFADVTLVDPKDYLEITWATVRSIVDEGVATRSVIRLKDIPNIGHLVQATVTRLTSKEAVLSNGETLTFDYAAICTGSSYSEAFKSSTSLNREHRLEELKAVHAEIRAAKSIVVVGGGPSGVEATAEVVEAFAGKSITLIHPAKHLLTTAPPRAGAKAKQFLESKGVQVKLGVSVQSQPVGRGAATLTLSDGSNLSADLVLWCAGAKPNTAYLGEDLAAVRDDRGLIKVLPTLQVEDHPKIFALGDCNNVPEEKKGFLATKHAELAAASIKALIKAARGGKSPKLGVWKPNMGMEMMLLTLGRNDGVCRVGGSVFTGFLPAWIKGKDLFIGKMRSELRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S545</accession>
    <name>A0ABQ5S545_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="616"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="737"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="833"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="957"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1130"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1181"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1256"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="483"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="738"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="833"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="849"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="884"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="971"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1195"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1207"/>
            <end position="1225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1276" mass="133699" checksum="E763D0892F95AE18" modified="2025-10-08" version="1">MKPSNLEQQLLQVHRAVQNWEHCKSDLQAATRALTNASGLLRWCLDTWPPAPTASALQPPGFSLPGLTSSGGTCADELPGDGDGDGAPIGGRNPCVINQSKQPPLDGTNCSSVGGARQVPQPLTATSDVHDWGGAPKPQVGAQELLALVCQELAQAQYLHALALCAAGYGAAADPWIAGLGYRLRLSDEVWAACADSVDHLAGAHAAGPFDAAPWSSDITGGMDSLDVSLSKSGNPVTAATSFAPKPFSKAAKIATAVAAGEYDLSTESIAPKPGVARALEGGTVGLVSDPVPRAVPPLICDDVLPIALLYRLAAAFDPAGTTPYWRQHRYGERETPFFSYLHRLDQPTPSSASCSGVVEEAIQLLYRRLLRVGPHQPGEREGKGKAEEYGDLKMAPATAAAAARGAVASELHGVVVAEWWAHTRDPTSAHQLHFDLNEDVLRRGLEDYQLAHPVLSSVLFLPPASPAIRACRHPEAAAAAADAAVEATEAEPGPGAGSGGRSLELRPLSRWERRCCPGGPTLVLDQTPAGGRMRVELPVDRGCGRVTPSSLSVSSSSAPNTSAHAAAITPITDADAAAAPTGGRPPLARCAWAIGPRPNRLLLFPGDLLHGVIPQPVPTQPSLLRSNPPSPPPSAPEQYSAPPQKQPFEDRFLNKPPQPGQSDSRCKASGVAAVAAPRITLIVAWWGAERLRTATSAPPLVRPGCRGNLGPQLHVARCPGGGAGDEDGLAAVTVTMGEGGHGSTPGEGSAGQEDQKRRSAAVVSDAAGMLETVHKVERTGLEAAGKEEDVVERARVRRRLMLQLAGAKVPTNGVSNSGGDARVDDLGDHAAISTNTNTAPITSGGSGSESDGSSSGRSSSGSSGSTGETPTSSGSASRSSDVSIEDDDEDDNDDVDRENDNKDEEDDEEDEDPEQHQYGDGETSSMGRVIRHRPYVLDWSHAGALRSGMLLPPLTTQDGLLPPIHERSSRPQSLDSESQQPGKVSGITPPGITLAESPERGNDRSGCISGVTRTPPGGSPPLTNRLSWRHWFAALRTRTSLVSANRMGVSGMESGEDGGTLAPLQWRMPLCISPAWVPVKRPPRDLRCAANSGAAATTSSAASVDAAVPAAAAAVDDSEFWGPAKVVAKEGSSKGVEPTEGDSQLLSSAPTPMWNSDWELGQQAVRGMAVALGLAPAMRTVKRHRRQQQASEIKQSDGAEGREEKREEEEEEQEEGDSGEEEEQPGVSGLRCVMASLGLPGLRVFLRHPRELEHLYLPPAPSSGIDSRTHQEKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5E9</accession>
    <name>A0ABQ5S5E9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="228" mass="25269" checksum="CE8A79E8050C3373" modified="2025-10-08" version="1">MARPHPKLHASLQGLFVSRTQFRDAAAASEAGASLSESGGDFVELALTREELPHDLNDACGRTLALVAGTPNARDKRRILEDHYSMFAIYETNLLWCPHSMSIAAWTGWVTLLTMADVEGIRCHRVVLRSVASETSSDVDMQMQMDVTFAWRPLHGLMAPLRHLLLTWGVKGPRLPSLTTEHTITLTINPQGRVVLHRDITHNFPCVPLLLKRVLGLATPFVATLLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5L8</accession>
    <name>A0ABQ5S5L8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008648</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051412">
        <property type="entry name" value="Formin_Homology_Diaphanous_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691:SF6">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045597344" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="715"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="734"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="764" mass="80018" checksum="B34A798508E94DC5" modified="2025-10-08" version="1">MRYHRLHQGGHLIIFLALCISSWGASRLGTAAERPAQATSASTRRQLASESPGGASQYAPVAFPVPCGEGYFLRRNGLPVDTIEGSCRDAATGAIVGSARLFLNSVVNTIAAVDVNELTFEIVLALPARTEIRGHLYDDASFAAAIYTGSGGDGGTTCCNGPPELPVVDVLRESMPNFLISLSASDLNLTSCVEKSLHLLISFSLPNGSVFWMAWDWVAAGDTVNNLCPNSQKAWADNHFSIMRCRCESTGLLLQPAPGPPLPLPPRPLSLPSPSPPNPKQSSRTRPPPRPPSPPPRSPRTPSPPPRRKPPPQPSPRPPSPPPNKPPSPPPPPPSKPPSPPPPPPSKPPSPPPPPPNKPPSPPPPPPNKPPSPPPPPPSKPPSPPPPPPNKPPSPPPPPPCKPPSPPPPPPNKPPSPPPPPPNKPPSPPPPPPYKPPSPPPPPPNKPPSPPPPPPNKPPSPPPPPPNKPPSPPPPPPNKPPSPPPPPPNKPPSPPPPPPNKPPSPPPPPPNKPPSPPPPPPNKPPSPPPPPPNKPPSPPPPPPNKPPSPPPPPNKPPSPPPPPPNKPPSPPPPPPNKPPSPPPPPPNKPPSPLPPPPNKPPSPLPPSPLLLIRPPPRPLPPTPRPPPRPRPPPRFTPSPKPAPPSTPPPKPAPSPNFPPSPLPPPSPRRPRSPRPPPNPSPLHLPSMQPIDSTLPFSPPSPRRPLARRNSPPPQRSSAPSFPKAPRFPKAARAPKPPRAPRSPPSPRPPRVPKPPRSAKLSKHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5R2</accession>
    <name>A0ABQ5S5R2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008717</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="113" mass="12158" checksum="734B2F85035E911F" modified="2025-10-08" version="1" fragment="single">HSRRDNCIPVCPISPRSYSLKGVLPARSASATDVMALDPTRCGTWECTPTPVAPYVAAAVATTEGEAVTMEAEVLASKEAQRPKADLLRLRLVVEQRDRHAIGGCRVVVSQET</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6D3</accession>
    <name>A0ABQ5S6D3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="232" mass="25974" checksum="18159180E0A6ACAE" modified="2025-10-08" version="1" fragment="single">MQRSIRPGRTAPTFAPFVTPLVAAILMVVVLMMVDPNNFQATAGSGGAHPSQHQQPLASAAKSSQSTSSRQRGTARTEASQVFRKLDTEPEADTDNPTRGALTEVLGDLLGTYIDGHENADEQEQTFMKSLTLTAMLHAAQSQAQQGTQPSPLPLPAWMANRLQQQRQQQQQQQGRQQRKEQRKPKPKQKQKQKQQEQWQVEQQLKPQQDGIVHLQRSRFLQDQQTTVVARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6L3</accession>
    <name>A0ABQ5S6L3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="121" mass="13510" checksum="4A914935AF6C0ECA" modified="2025-10-08" version="1" fragment="single">FEKKPWKKLWNKESWAAWRSFKYAQPDNPDAGPSERRIVMEDLNVTDRQQELINTLVQRKQIESSKTSFAVPATPVPVGAGGTAQHTPGLGALELPKGTRKSGRNDEPPGTQDGRTSKRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6W6</accession>
    <name>A0ABQ5S6W6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008656</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="179" mass="19939" checksum="BE3759FD2AD15381" modified="2025-10-08" version="1" fragment="single">MGNSCSSAEVVALRNGSKLTNNTGNIQTSRSADGGLNDVLGHRGSAHISRAAVSLSWLKHFVKTHRLDETRLTTAQVVQQLVKPPTATRQCRFTHLLLENDPSVQKARITTNSTITMLFPRTDRTIRQPCLEQALCRDAGDGGATFKPEQQRFWRRRRQQEGREEGRGEIVLAASMVMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7W6</accession>
    <name>A0ABQ5S7W6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit</fullName>
            <shortName evidence="8">Oligosaccharyl transferase 48 kDa subunit</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_009718</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DDOST 48 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005013">
        <property type="entry name" value="DDOST_48_kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055459">
        <property type="entry name" value="OST48_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055457">
        <property type="entry name" value="OST48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23358">
        <property type="entry name" value="OST48_MD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03345">
        <property type="entry name" value="OST48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044992790" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit" evidence="8">
        <location>
            <begin position="22"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 N-terminal" evidence="9">
        <location>
            <begin position="25"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 middle" evidence="10">
        <location>
            <begin position="287"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI66015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="425" mass="46891" checksum="6AFE39362AF7DB9D" modified="2025-10-08" version="1" fragment="single">IMARPLLLPLVLLAALGVTSASKSVLVLLQNVNTADKYSKFFGSLRDYGFDVSVKGVKDSTLKLKEYGTWLYDDLVLFAPQTEGFGGKIDRQVILDFVDSGHNVLMALNSDASESMRNLAAEFGIDVDDRGSKVYDHFSHATLNGKEDHTLIAGGNIVDVPVMVGGPYKEPVLFRGIASVIPGDAELATVALGAPSTSYSHAPKQAMLEPPSLMVGSSISLVTAVQARNNARVVVAGSLEMFTDDFFDAVVTVNGVSTHTANWAFTVTLARWAFQDRGVLVASNMRHHLLGSDEQPWGYRVNDDVEFLVDVVEVDGGVPKPFAADDIQLEFIMLNPYIRQPLQHDGNGTFSLRFKVPDVYGVFKYYIDYAHCGYSYIKLTHQVPVRPFKHNEYERFLVCAYPYYASAISIMVGFFALGFFYLYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8M7</accession>
    <name>A0ABQ5S8M7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000029">
        <property type="protein sequence ID" value="GLI66257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="113" mass="12207" checksum="F47B393EF1305421" modified="2025-10-08" version="1" fragment="single">AHGQALDAADDYIFLNDEDDVHMAERMIRFEEPVQAESVSAALEPPAIAPAAIQPTTPTKLEERLHYSYKSRGSKSTAISAPCSWGIRDTFVEVFGSRICGQASIAAPSKSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S976</accession>
    <name>A0ABQ5S976_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_010320</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF133">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="5">
        <location>
            <begin position="72"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="511" mass="55254" checksum="E608CFEAC739FF10" modified="2025-10-08" version="1">MHLQQLGHPPRAGLANVSADRRVLIAPRAGSVAPTEPAPQQQRQQPQQQSQQPPAVILDDSRTQAFMSWARGPASIRFSGVRPSTFAGVRGLAASSDIATDSLMVEVPRRSAVVLAPKQRNPCPDMVTDDWWKSAPWFAKMAAMLMWHKRRGSQSSLAPWVAQLPADTGVPVRWNEQQLAALQYPHLISQVTEQQREWQQLYDDLVSKGMPSGAQPPAREDFIWAMSCVRSRTFSGPYVGSTLQDRLRTAGLVAVLALGNTVLGLADPQKTLSAAIAVLLFNVLYEVILSRSLKQYAICPLIDFFNHSSAVQSEVSYNYFGDSYSVVASREFTKGEQVFISYGPQSNDSLMQYYGFAEANNPQDVYVMTDMLRWVTAVRPVGQARLDALKASALAGSLHQVVIQRAGFSAGALQAVRFLLASDMEAAAGISSFAKAGSPNQEAQLALVLAEVVRRELGNLGSSLQEDVALLNGGSGGGGRKGGLSTVEAAAVAFRVEKKRVLMAVLEGMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S978</accession>
    <name>A0ABQ5S978_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Telomere-associated protein Rif1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="922" mass="96318" checksum="3C2C9E5E0FDB993C" modified="2025-10-08" version="1" fragment="single">EAKLHDVRTKLESDKVTVRKDGVKLLFALLDGQQTLQLLDSETARLWPDEQCKKLSSWPRLLRVFIDYTERELLLAASAGSGSGSGSRSKGGAGSRGGGGGGSAAAAATAATKASEAVHWLRRFVATADDVQRSGRRGNLQLWVWRLWHHVAKMVEAPGFFQSRWAPDYLALLSRHLLASPEYSRLLEPVHAHTLLTFSCGALDNLVGAHGVADSTTATVTSQAQAYLGLMTSLLMVMPGDLLDEQRLSIQASLEASARWLAKIRDTGRLASSWFSCAVQLLVTGGLDGLGTMLPQLHNAVRPLMRLAWRDDRASRLKETITRYCLLQLQLGGLVPEHESESSSGSGGSSSDGGCRSSGGGIGALAELYDMTVNDVSCGVAKWDLDPEDGSLGSLSLLQLQLLAGLFFQMQQVHSAGRLRPEHLTYGSDGAGAGDDGRSSASAMVVLDSDDDDDGGGGGGLGGRRYTAPPPAKRRRQLPPLDWLLEQCATTPSRWAPVLTALLHHHGYHLPPTFHFAALQQVLTCLRALVPFAAGTVSGSRHGANGDAAGVAAATAAAAAAMLRPQDLVTKQAGALWCLRCLEILAQQHVYGASQTHGSRFFGCGHSVSLATTASSSSVSGTATSPSGRDSLAAAVTVAAAWQDVAQFLTLRVAGRNLLVPPSQGLPNLLQAEALWALTAVLQLRRAAAASVGGGVRSYIVRPPAGLLQTLAAVMQASIPTDDVDPAASGGSRTPQTGVAAAATSPVARSFVWQAAAAAVVAALNAAPAADAAGAVACKAAAVSCQTAVLRLAITPPNELLHELLGATSGASYTAVNAARDANATPDLPPPPPLPPLPLDLAPEQYPHPYTRLPRWGPNDSETTAFCTNVLAVQRRAGDVMAWQEAREHAFRYLTHGSVAGPGSVATVAGAEALAAGRRVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S999</accession>
    <name>A0ABQ5S999_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_010140</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009316">
        <property type="entry name" value="COG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024603">
        <property type="entry name" value="COG_complex_COG2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024602">
        <property type="entry name" value="COG_su2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12961:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12022">
        <property type="entry name" value="COG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06148">
        <property type="entry name" value="COG2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 2 N-terminal" evidence="10">
        <location>
            <begin position="29"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="COG complex component COG2 C-terminal" evidence="11">
        <location>
            <begin position="445"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="567"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="575"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI66359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="872" mass="89377" checksum="1418C38E538C1C30" modified="2025-10-08" version="1">MNTMAEETAIVTATSDSSLLLGRAGCPVWLNPERFLVSEFNADACVADLRRYVPLATLQNELSNYLATLKTKLVEVINEDYADYVGLSGRLANVEGSVVRMRKPLLELRDKLNTVQEAVRSELNSLNQGLRKRKEVATQRVQLELILEVSHVAAKVDKLLEELAASDKTTSTATAAATSVPGAASGLAAATDITGSGFGPSDAELLAQCRLLERVAAEVSRLAFLANKGKDLAFVRSLEPRITAHRAALRGRLSTALALALTPAPAADAGAASPSPSPRSTAAALHALHAAADLGEAATAEAVVRSVVVAPLVERQVGEHKAQGHTSVPVGGAGSGSTYGNALSALLSGILAAIRAQLGPLLDASLAPGSALRSIDLLSNAVLDEVQGALAAALPGVFSPGVPPAFHANYLATLAWLRQLEGVCATRGSVERLRSSAAYASLMCRWNTSVYFSLLYQEIAGELEDALSADKLEPAAATAGSGAGLFATLAPPQLTATAALLRCLRRAVSPDVVLPLLTDRFLRLALQLAQRYAAWVSAATTARREAAAAAAAGAVAGAGTAPVAGAVTPPPAQGAGSGGAGSPGSGGGAGPSGSAAWAPTLPADELFAVVSDTLVAREALLGPVTAAFLELLPPTSMPSHPAANDTDAASNGDGGTVAESAVDAAATAVRGALAEAAEAVRASGCGLAAVVTEEVVERCAAAVRQLKGITATYRVTSKAPPVRASHYVFGVLMPLRQLLDAEHVKRLNPALRQELVTLPVAESVCARYAALAEELLVSVRKTESSLKRLKKAKGGAGGEEEPAAGLSDSDKITLQLHLDVEELAVQLRQLMMQPDTAPEARLPSLRRLKEVVAPAPRDAAISGAAGSGGGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9A5</accession>
    <name>A0ABQ5S9A5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021883">
        <property type="entry name" value="LPA1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35498:SF1">
        <property type="entry name" value="LOW PSII ACCUMULATION-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35498">
        <property type="entry name" value="PROTEIN LOW PSII ACCUMULATION 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11998">
        <property type="entry name" value="DUF3493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="323" mass="35498" checksum="2383E83D00FAB215" modified="2025-10-08" version="1">MICELNMQLSSSKPSNLCSSTASYKRCRVRAFQMRRTRGWQPCLASRSDGERTQPSILNIRGKEDAVQMRAEAEAPFRSLRLVFSGFSVVSASLGFLISIPQLIGTLAGTRNALPLEQVQQNLVIDAAAIALFAFLFKLDWEAREKQLARLQREETLGALPIRLASGKTLTLAGLRGSARVVLAAGTRNQVATALREAEPFRDDLVKRGVVVVPVVIFGEGSDATSSPTTPHDGELPQLSPADLRWRADPQRLGDWQAWFKQQLSYSAKAKPENGLFVGLRLDGRVRSSGSGVPPWRRYALELAPLEGDGKWTGFFDGFDGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9L3</accession>
    <name>A0ABQ5S9L3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="1">
        <location>
            <begin position="60"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="1">
        <location>
            <begin position="385"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="552" mass="58821" checksum="C915E03116D24063" modified="2025-10-08" version="1">MRSVRQCCLHDIRPPQSPAAQWLPSYPDTTELFMDKRAAGVVAEGDYDTSVDQTGFLPYFPFRECASAIGAYRFAPEVTQSAPGKYCFTVKVQTQNCNNACCAKDLYKIEFNVSDSCLVQPAGEVKATINGVPTRVGATYDRPVNGRNGSAILRLTQLGLNLTTAADAQLCLTLKTNRAGKGCGTLEQMCVPPPGAQPGACRAAFFDTSCKCCPVSSTGASPPPPPPPPPPPPSPPPPAIPAPVVPALPPRCEVCITANLLPPAVDIRPYRYNADTCASIQQNISTSINTALNNSNIRMVSFFKTNASLCSALEVSVCGTFFSYLDAVSFTTTAEELLSFWISEAAGGNVCQPELEGYTVTVSTGDSSCLPVSGSIGCSLPFTPFPNCTCNTSQGILPFLVQPRYLPLPNVNKTTTEYCFLVSTITAQEIVPSTCGVANDNLTKIEWYANQNASSWIKGINLYPAVGNPTKLSANWGAAGTYTLKATPINWTSAQANGAMVCIEVKKPKSLEDVCLGFSGQCYASTFNSNKDCCPIYRTGLTALLPVVGHHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9X5</accession>
    <name>A0ABQ5S9X5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="524"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="731" mass="73254" checksum="F818E956907CCBED" modified="2025-10-08" version="1" fragment="single">SSSFSSRSSLSAEEEEEGAPYVVPTATAATATTAAGATITATATAAGIAITATATATATATATAADAMANGKPMDSVTPAAAAAAAATNAHADANTNVSASGSAEGIALGFDQDADPATTDTLLHTTISPSQSTAESPATLTPSTGGDGPSVSGCSCGAFTGVGADVLKAPDLTGHGAGGGHAAVADGCSSSMSSNKWQRLRGEADGTEADMQGQMMQVLLRVSPTEVRRVLVKKQWIRRKLSGGAGIGLMLGLGGGVGGGSSGGGAASAVSCSDSSSTIHAVESYSRNISGSSNNINDNDNDKNRDGCTGDGTEPQLRSQSQHSLEAAVAGGAKWIPFRTLKDGSGATVAAVICPQSSRVRLGPSRRSSAALTQGLARSHSAVSAVEALASLERRMKVVIAAAGAADEAANASITVAAASRHPLRVLMPVPSVRLHVRPEAFFNPDGSLLKGAVLQSACHVIRLAQIRNQLTFVAPGPPTSSVSSASPSPFPSSTPSASSALTGGQSPSVGEAGPPPSPFPPTSTTTSAPSSSDSASDNRRHVPVLAVLKYLAEFVAAARALEHAKAGYGCGRPAALFAAVKPQLLEEVQEQQPRALDVVSGANHGSVNVTLVQVEVVTGLAAAATATAMATTMVTATESAVKAPDTASTAAACEKQPQPQARLQYQNIFRPGVQLPSPNGVAGAVPVAAVESRGALYALQPLMQRGRESAAVLMEAAERALACRDKMWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA10</accession>
    <name>A0ABQ5SA10_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription initiation factor IIE subunit alpha N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010751</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription initiation factor IIE subunit alpha N-terminal" evidence="2">
        <location>
            <begin position="60"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="559"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="587" mass="60900" checksum="C68E008E1E69510C" modified="2025-10-08" version="1">MSFASGGPVSAASSGYAPATYQRLMRLAARAFYNGPCPPPGRDADPFSAKSKIAKMDTRGLGVVLIDYLTTVEWTSFDLLVETLRLHPVVLSRALRFLEVGHMIRRYERRESRRKKRNLVGDALEADVAQGGDSDEEEEEEEGGTKKKGLLTEYFTLDYARAFDALQIRIQSMRASLKEQLEATNAVQAYRCPGPFCGKTYTSLDAANLVDPTDLTFKCEMCGSELVEQRATNDAQSGSGAPHHATARESKEAARRLLQAIDRELAPITRIMTDLQHRAVPLPDPGELYEWAQRVRQREADMAAAAGGGAASGAGRRPGGAAAGATHGGGGGGGSGAPAALLAGSGVTVLSAADNTNMWEAQDFKVSLDGGAASTGGLLLGGAGAVASVDGGGGGGGGGGKKQSTALPWFKKPADEKARSGPMGREESSADGGGAAGTGAATSGKQQQQAEYFRDFMNYMSQLQHEKRTPSTPPAPAAAAAAAQTSPLPPQPQPLAVAVKPEPLGILAPPGPLAAVVKQETGLYGAAAATAAAPAGDVEWEDVEGVPPVPQQPKLEDAIKTEPGMGSFGAGGSAAMDADEEVEWEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAA6</accession>
    <name>A0ABQ5SAA6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA:m(4)X modification enzyme TRM13</fullName>
            <ecNumber evidence="12">2.1.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_010514</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">tRNA methylase which 2'-O-methylates cytidine(4) in tRNA(Pro) and tRNA(Gly)(GCC), and adenosine(4) in tRNA(His).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>adenosine(4) in tRNA(His) + S-adenosyl-L-methionine = 2'-O-methyladenosine(4) in tRNA(His) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43196"/>
            <dbReference type="Rhea" id="RHEA-COMP:10401"/>
            <dbReference type="Rhea" id="RHEA-COMP:10402"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74477"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>cytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43192"/>
            <dbReference type="Rhea" id="RHEA-COMP:10399"/>
            <dbReference type="Rhea" id="RHEA-COMP:10400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>cytidine(4) in tRNA(Pro) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Pro) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32767"/>
            <dbReference type="Rhea" id="RHEA-COMP:10397"/>
            <dbReference type="Rhea" id="RHEA-COMP:10398"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the methyltransferase TRM13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.225" evidence="12"/>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007871">
        <property type="entry name" value="Methyltransferase_TRM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039044">
        <property type="entry name" value="Trm13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021721">
        <property type="entry name" value="Znf_CCCH-type_TRM13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998:SF0">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05206">
        <property type="entry name" value="TRM13"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11722">
        <property type="entry name" value="zf-TRM13_CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="8 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="14">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="439"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="486"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="616"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="812"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="530"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="616"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="626"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI66594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="855" mass="87497" checksum="F9DACC7A5F9764A0" modified="2025-10-08" version="1">MITKGSAAGSPHDNPELGRCHFFLGGRKSRFCRTLAIPGKRFCGNHLHVGDPVAAPTAPQRVPCPLDPNHTVLASELELHVSKRCPALALALRERAQPHFFEDVNVGSDAEPNLPPPPPRPPTRAAVVAIGAGSALVDTTAGPASGPGPSTSASSAEAVAPACLSAASIGCCRSTGPGIGGSVAILADDPGDGSGSAAKVPAGTPRSAAAWGPPLGPFQRGRAAQRAALARSLGEEAFMKLLARVEAAHTEACGPDLLVDCVMRPAECEEMLAAPSQLRPFDLKHGLQQASIVGNMQRVGLISPGQNGGAGVGVVELGAGKGYLGATLASSCGVDRLVATDVKSGFKLKADRRVRHIVFGRYRVDLKDYVPSATPELASAPPGSPWVAVAKHLCGAATDYGLRACITQDPLLGISTAVVGVATATATTMKAAWAGVTENSECKGQSGSGPAGRKRRRRGTREAEGLGRSEGALVGKELCKALVTESADGHAATGGPDTTQPYEPQHQLRGQEQEEQLHPQQRQKVPEGSGQPSVSSGNAGYSDGSGGAVLRGLAIAPCCHHRCGWRAYTGKPLFRRLGLSATDFELISWMTGWALCGHDTPAGAPVAEASAVNDVDCEVDGDDGGNDGGGVGTGGGGATISGPVNGSPGAASKTDCAPDSRTQPSPPLPAPRESEGTLPARNEAQDGEGDGNGTAMTPVVVAAADTELRPAALAAGHYVMDSSSTLSPMTLARGPTPEPLSTATFDPVFRLSRALRMAVGQKCKQLIDAGRLDWLRRRFRIVELVSYIGPEVTGENRLLLAVNPLWAPGAGVGSSGGRGDDGDGWPGSEGAAGLADPPVRPERRIEATGEGNAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAR0</accession>
    <name>A0ABQ5SAR0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1350 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010765">
        <property type="entry name" value="DUF1350"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34127">
        <property type="entry name" value="OS04G0405600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34127:SF1">
        <property type="entry name" value="OS04G0405600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07082">
        <property type="entry name" value="DUF1350"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="39568" checksum="42EA78CB296409BD" modified="2025-10-08" version="1">MIPDFTVRRIRLGKLVTARRIPAVIAVRRRAARCLASTDVVKLDTGAWVVAPRYRSKGLVHFLGGAFAGAAPQVVYGFILDAVAAAGYTAVATPYAVTFRHDECARGVRQQFLDSVDELRRSGRGDWAPLEAPTFGLGHSNGSLMHLLIGSFFPGVTESNILMSFNNKQVKDAIPVPGLMENLPGALQAVRTVAPGLPDLSAVPGLSTLPLPSQLPSGADLLRGVASLLPRELGLDTAGQLSGAGLLIDQIPSVFGEVGGGATDFYPSPAESRALISAGYSVRPTLLVRFSDDSIDESAEIARLLQPRLGSGVTLLNLPGNHLTPCGGDVPWPTGPVFGPADVLVQVLKEQQQNDIQRLCRQVVGWMEAISTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB12</accession>
    <name>A0ABQ5SB12_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011336</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="66"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="361"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="413" mass="46207" checksum="DACC746467235BB3" modified="2025-10-08" version="1">MCHRSLQAGPSLHWSHCHNRLARQRPCLHVRVTTTAVPAMSISTSTSSSDASLFELGWRTDFRETYVQGKVLGTGSFGKVHLGINAATGENVAIKILPKERPRLSRAKVLEKLQRELALLDRLSGSSRVVQLLDCYEDEENVYLVTEACQGGDLQKFSDTYGPLTERCLALVALEVLHVVHSCHRLGIVHGDVKPANFCIMDEHRHPYAATAAALQRTPWLKAVDFGCSQLHYRHTRLSKRSGTPVYMAPEIFKRDYSFEVDIWSTGVLVYQLFCRQFPFWQGDSYNKALSLDEVAKAILEADIRMDFGPWLTMSPEGRNFVRGCLTRDPTLRLSLDEALEHPWILAHTGQEACSGSAGPLVEAELSGSSGNNIVTRTAGPPQPHHQQQQIYEHAQQQHRQQRHGRRDAAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBK0</accession>
    <name>A0ABQ5SBK0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-(apurinic or apyrimidinic site) endonuclease</fullName>
            <ecNumber evidence="6">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011462</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF4">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00728">
        <property type="entry name" value="AP_NUCLEASE_F1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="8">
        <location>
            <begin position="24"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="315"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="402"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="480"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="413"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="600"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="625"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="656" mass="69194" checksum="9200A67D683BDB60" modified="2025-10-08" version="1">MERGPRGTATEVEPGAGVLKIICWNINCLTPTVRNMELRHKSFRGFLDHLQLDIACFQEVKLPATRITRDLACIDGFQSFWATSTARPGYSGITTWCRTATAAPRDAAADGLGGDEQPELDTEGRLVLTDHGAFVLLNVYAPNAGDRPERVRLPYKLAFLRALRAKMDELTALGRKVIVVGDLNVAAERRDVHPSLNFDSMYDEEELAVLAGLRTAYIDVWRRLHPDVEGSYTVWEERTSARAFNVGLRIDYVLVSPELLPYVESCEILTAAEIPPKWSDHAAILLTLRVHHTGSPALSPQSQRSEHPDTAATIAAGSGTSSVASRARGEPRVDASHRSFGGAGGDAGLAAADADASSGGSGEGSLPPRLLLSPLPAAPCVLWRDLEARFLDRNQKTIRDMFGRKQPIGLGSRPQTTARSSLATVAVSEPSDSDAAGAGLGRKRVAPADGEEAALPPPPRPAPALRTQNSNTAIMANAGVQRLPSKRQEERAQEQSCDARAGSTGNDTTTDHGAADVGGGCGDREARPKQSRPQDQAGFAGALASASEPDDTGLHQEGDGPAGAPPVMVELGDVTGDGSCGSQQATVDLAGVQPLGLPQGSASASLQQRQLESSRGRLSRKGAPVKGGGATGRGSGAAVVSVSKQKNIQSFFKSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC06</accession>
    <name>A0ABQ5SC06_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="32532" checksum="1D284570DCE9DFD4" modified="2025-10-08" version="1" fragment="single">MAEPAETADPLEGNCPAFDLLRIFDSIVLPSAVLSHMDLNTRKLLRLCCKRLRADVDALVTSITINRYNIPKLQPTVPGSLALRYPRLRRLAFGANEPRDVAALLRAQLPALASSLDCLDFWSYYASISMDLWTALVELAPTALRLRLNINESLLRRMEVATVLAALSTLRAGRPGLVIEFNTGHTSFGFRSTGPETLQFLSSADYVRSLGVSIGLHGRELQDEHFFDRIAITGAASSPATAVAAPSRPLPSPAATSIEALSLTHYEAAERPPVGPLLRGIHLLAALRDLTLDCEITLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC87</accession>
    <name>A0ABQ5SC87_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011738</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF66">
        <property type="entry name" value="CARD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="588"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="853"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1073"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1181"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="79"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="133"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="247"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="712"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="884"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1232"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="590"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="606"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="855"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="866"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1074"/>
            <end position="1090"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1286" mass="135762" checksum="250E88DBB6AE668B" modified="2025-10-08" version="1">MSDSLEQLQERERELTELRNRTLQSLEQQVAAREREINTLNEQLEVLTSDFTYNLDLLRQRDEELAAYDEASVQSQVQETALRGRVTELEQLVTELEAELRSARAAAEQQSFVLVAKRAELTQLLEEERLGRTEALMRQREQAETVRRALQRELEEARQQAERQRAALNAAFQQQLQEVQAVMQAEVEQLKQAAATASQLAEQRQQELQQVAAREAQALQQADAARATIRDHEVALRGLDLELEARSREHDATVRQLQQQSTDAERKLQAATESYELYRSQLAEQLSTAQQCLEELRIRCLEDIALLQMRHGEEVVALRSRMEAATGRNAQLEAEVSELRTQLRVVKDEVAAFELQLGTERAEAAALRRDRNDAERRLRDAASAQGHEIRQLREKLTEARTSLEARHRDLEAAREQLVAFQQRGAATREAAAQAGFAGGVDDIAASGTGTTSAATATVTTSNLEPADGELPPSPSRPGAGGLQRQYMAGRPTALTIAALQESHDSLPDFGPSTLLDLPPSPRSSASFWLGPLSAGGAAVGGLQDSFPRSSWSPAAAATAAAAAAARAAQFLRSSKEGLDSLLRPLQRSRLGPAEGPQQQQQQPGAAGAGSQGTGTGNSAAAAAEAALAEAEAENQRLRSTLAVMRSEMEALQVAAGAAADSVFMQQQQGMAWPVGSGGGGGVLETGEGPRDVSIGPGGVGAQLNAAALMAELQESDRDMQQTLEHMELLQRQLQTARAAVNTATATAATASGTTIAVTMPPGAAAGVGPSTASPGQRATAAAAAAAAEVTFLRQRVKELTMANRILRRRVMQSQAATAAGAAVAAEAAGTGTVAGFALFDMLGALDKGNTVSADADADPDADADADAGATRPTATAELPMAEKVQQLQQDLDRSRSQLAAARAENEKLMELSGEVRAQRDRYAMQLAALMSTSAAAAAGGALLPPPPTDGELNIPGLPWRKQDFMSPPSPTFGASLPVNSPLRSLPLVTAATAASSLPSSPIQTSPTSLATLLPVFVPSQNPPVGGSIDGGLYGYQYPYGLVSPWGVLPAGGVLPGSGMPYWLPVQPLPGAADGADRVVKGGEQRGRESADSGAEEGGSNHSGKPAGRSTSPPTRRVAFDDARSLRRSASQSPPRPGNVPSHSTNANYVRHNQMRLHSNPDPSHSRSLVTTFDLAAAAAAAAASNTEGGEDRPPGTEATLTVGPVPAAPARAAPPLGLTASGRETQSQRAALRALHRRREQHQNQAAAAVATAAALRQRVRSYTVVRDEGGAEDAGGVTTTSWPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC92</accession>
    <name>A0ABQ5SC92_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000057">
        <property type="protein sequence ID" value="GLI66952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="177" mass="19345" checksum="0F72819FE96592CF" modified="2025-10-08" version="1" fragment="single">GNDAQQRKEQHQQQGTTAAEDEGEGEEQRQQPKRSNCKGASERLIDMERRVKEQDLEASEGHRIDGRLWGAFHPAEAAVGEAEAIMQAMETPADEQMQTEDDIDDIEMERLRRHDTPDGSDMERQHRAAAVGLTSLGLLAGSSQGPPTAGKSSKATSGSDESSLGRETEDEVMAKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCI1</accession>
    <name>A0ABQ5SCI1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MPBQ/MBSQ family SAM-binding methyltransferase profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011886</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044649">
        <property type="entry name" value="MPBQ/MSBQ_MT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031164">
        <property type="entry name" value="SAM_MPBQ_MSBQ_MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44516:SF11">
        <property type="entry name" value="2-METHYL-6-PHYTYL-1,4-HYDROQUINONE METHYLTRANSFERASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44516">
        <property type="entry name" value="2-METHYL-6-PHYTYL-1,4-HYDROQUINONE METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51734">
        <property type="entry name" value="SAM_MPBQ_MSBQ_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="MPBQ/MBSQ family SAM-binding methyltransferase profile" evidence="2">
        <location>
            <begin position="61"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="334" mass="37443" checksum="04CF1ACFDFF5B3E8" modified="2025-10-08" version="1">MLAGSPQLVPGHRLAEVSSSRPRSIFRVGGPLQRHANFSSQRILVRRSAAETYSRDVTAPRLIQHKNEAYWFYAGLSQVYDHIVNPGHWTEDMREEALAPAKLDNSNLRVVDVGGGTGFTTLGIIKNISPENVTLIDQSPHQLAKAKAKPALKGVTILEGDAEDLPFPTDSFDRYVSAGSIEYWPEPQRGIKEAYRVIKEGGVACLIGPVHPTFWLSRFFADVWMLFPKEEEYIEWFTKAGFVDVKITRIGPKWYRGVRRHGLIMGCSVTGVKPKAGESPLVMGSKAEVSDKMNSNPLSFLLRLILGTAAGFYYFCVPIYMYLKNLVWPKNWPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCU1</accession>
    <name>A0ABQ5SCU1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="747"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1316"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1565"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1664"/>
            <end position="1701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2270"/>
            <end position="2302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="756"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1338"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1594"/>
            <end position="1614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2423" mass="256754" checksum="1A57841316118128" modified="2025-10-08" version="1">MAKCRGEMQSCNRQYHAIRIPSASLSDVKIRSVFSAALSSEHPSTAASALRRGGGVPLAVPHRHCPLRCTAVNTPPSRSFSSFDRRPCVPMSRTGREGTSGQLHGSYVGAAGQRSSDDDNVGTVRVPLWPTSPGPYGRPVGRVLQVGAPLELSSTSHMPVRFIVSHLVVSGGLLNLVLEGRDGGGAHVRLPLPLPLPSQIRFYSADGAIVEGHGEACLPRFGPSSANSQPGCGYSGTRSNSNRKTLGQQQPQSPKTKLHYRVHDSQQQQQPTRPYSKLIAPNPSHVSIGSRGVQTIPPQVTLTTAECASAAAAAQASASTSADQGDAHSTPTLSNTDIPVRQEAAGEGRTGLSGVSAVRAPNGAALPGASVGSCLRGSTALKAAFPNWPPPREPVGNARLSIARPKKGDGGSSSDGGPANDVRLLSRPELPSVARILAGFMPMRLVRRSGILYLSHRDTRLRWRTKGPKGQHAPGDASPAAGEGRGHSLREGGGWDVLKLQQLSPYLQLLRAATPKLGKSVTPAFRGDTVAAGGAAAQGGGTPPQISSRGPIAAVRTEGIGSNGAGVRDRREHAAAFRPTVMVSRRGSISVCEAWRKWDGAGAGVMGVVEDAMQRPDVSQTDGGGLQSDADAVSVRPPGAGLEVRPPGAADDVRGPWHSALLQILRAANPRGPRAPIAPSHRLSDFAERATTAAEVVSGSRFARAASLSAKAAAATAIAVPPSARQNAAATAEPSVRAAATAAAEAAFPGFTPDTSRQRDRRSWHEVDDPLTPLEASVTGATSLEHLYGILDVHWDSLEARLIVLACRVLVGLTLQSGLTTSQAVIGPGTARTAAAPPWYSPITGAVHCYASVLDYHRTVSKRPGPRTTRAAPGGAQPSIWGEECITHVAGEQRQYSDATGTRTVAGEQGPRHGLLESSEHLELLSLARRCVGRLARRAAALVGDHHAAVAAVPKLGESGAKELTGRLVSCDRRMALEVSLSVLWAYGHLADPEDRLDLQLVSELDLWLLVGMASGWTGDARSRTWRPTEQWDGLLHLGAGPGDAAALGPTMNPTAIADPTDMPFRGIMCRLWRVVLLGLWAARLVELGSQGAGHLPGDPSRASSPIRIPQLQQWIMRALWASERGAPTLLPGVLLTLAAELPQLLPPSQSLVEQQQRARQPELTTTASGGWQTDAKCAGGCAVPQRTEAEMPESGGVSLPVLSQMSAGQLVSVLWACSRYAGWLEERSSRTRRKKYRVARYGWLERRRAAFSAVLGGIIGGNSVGGRRDVARSLAQQMACVAASALRLLAARVASQPGLHVETVLDLVLDLPQQQQPHKPSWQHHHRDQQRRQEGQPPAAMSGGASPAAAPSKPAGDLLSRQDLLAALEGATTVLEGMCFENEVLTLVTALSTHPAVRLQDLTAALRMLQDWLPPRTHTPQRYKPGAIPPPAVTAATTHTAAARPQHHTHHHDGSHAVVAGSAAPDTLFDPLAFPGGARPGAANAVAGAAPSGAVQAAAGGRPPGGPSDAAAVLLGLEATVLRLLGDMRGEEAEEMLRLQSLGSRGLRPRRGWYRTHLRLPRLARGSSMGTTERDEIHQQQQQQQQPNMRHPVFRDESQGFHHQDGQDSDASRVGDSGPIDRWRHRSGGGYSPQQSLMRLTRRLAHPLPLRLRLLYRLGPSESAGNEADRGWAPREGVCSPADGSVTSTDGLERISGTADGDRNTTTAAVAIGGNSNCGGNMTRRGIGTHMRSLELQLRHQQRLYEDETVWRYGTSDLLDLLEAMGAAAKAGCPRPRCVTVRCLLNALALRWLRSGVVQGDVRALIATLCRLGWRPRAAVTVFTTAWRPFVVLAPQSVSDAAVAGMAQVLGCLISHDFVCHPGGRAAAAAEGGMAWPKLRRFWVNLLALASARLQTGGLQLPDGAVVKPAAGGNPAVQPVLSDATATGTSAQYRSVGLDESASAIIGAGLRARAGASGADGHAVVPAPSLPPSALSARSLVRLVWSSGRQGARVPQLLGTACRVLLPWLPQLHPGVVGALAWGLGQPRFCAPRMNTALLSLLRRWLVAAEDSGGGDDTEGYDCNWTAACRIATALLQLRHISASTPELASLLRHITSRLQRHNRYRNRGCQRSPYSQAAHQDYSAGGGWGRTQASMEAVRRGRGDIWSWPDGLAPEMAATSNRSPCCRTAAPGNSNKWKAVHGRLGSTTGPLSLHEVVNPRALANLLWALHSELGPIHSASSTARPGGRGARRRLRLLASRSAPIVGCRAGPTFSSPSQRESTASLSDSTACAGCAPTGSDSPVSGWGIRTPAGAADGQSLRRRGPTLLEEHPELFRSAAAFLARRSADLPPVSLLQLLQIMAFSRPPGRLVSPTFLDAFGRLVEVRPRWLLQSEAQARTVLECYRRLGWWPVGQVEEIGSRMEELRRAPWRHKPQFGDGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCU5</accession>
    <name>A0ABQ5SCU5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="6">2.2.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_011093</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI66996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00315">
        <property type="entry name" value="DXP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049557">
        <property type="entry name" value="Transketolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00204">
        <property type="entry name" value="dxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003933">
        <property type="entry name" value="PRK05444.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="1-D-DEOXYXYLULOSE 5-PHOSPHATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322:SF5">
        <property type="entry name" value="1-DEOXY-D-XYLULOSE-5-PHOSPHATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="13">
        <location>
            <begin position="404"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI66996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="735" mass="79046" checksum="6C1FF86591F55278" modified="2025-10-08" version="1">MLRGSLSQCPVAAERAPASSSRCVAPVARSTKSVAPTRRSLVGNLVNAPRAGPVGSYSGEWDKLSVEEIDEWQDRGPKTPLLDTVNYPVHIKNFNLEQLKQLCKELRSDIVHTVSRTGGHLSSSLGVVELTVALHYVFNTPEDKIIWDVGHQAYGHKILTGRRKGMSTIRQTNGLSGFTKREESEYDPFGAGHSSTSISAALGMAVGRDVKGKKNNVIAVIGDGAITGGMAYEAMNHAGFLDKNMIVILNDNQQVSLPTQYNNKNQDPVGALSSALARLQANRPLRELREIAKGVTKQLPDVVQKATAKIDEYARGMISGTGSTLFEELGLYYIGPVDGHNVDDLVAVLNEVKSADTVGPVLVHVVTEKGRGYLPAETAQDKMHGVVKFDPRTGKQQQAKTKAMSYTNYFADALTAEAERDSRIVAVHAAMAGGTGLYRFEKHFPDRVFDVGIAEQHAVTFAAGLACEGIVPFCTIYSSFMQRGYDQIVHDVSLQKLPVRFVMDRAGLVGADGATHCGAFDVTYMASLPHMITMAPSNEAELINMVATAAAIDDVPSCFRFPRGNGLGLDLSQYGITKDLKGTPLEIGKGVVRRAGKDVCLLAYGSSVNEALAAAEMLERDGVSATVIDARFCKPLDTALIRTAAREHPVMISIEEGSIGGFAAHVMQFLALEGLLDGGLKFRPMTLPDRYIEHGDYRDQLAMAGLTAQHIASTALTTLGRAKDAAKFSLSALSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDB0</accession>
    <name>A0ABQ5SDB0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="209" mass="21814" checksum="262528180D5ABAD2" modified="2025-10-08" version="1">MLCRMHTLLSGGTDLTQKIRKHSNQCSIGSHLLRSGTSVSLVATTSKCKPRSAICQYRQGDEAQDSTSTAPISPKPATAAPVAVPDRAPQQRTAQLKVIGLGLRGISAVNRLIGSETLPEAEFWALDSDKRVLSTADATTRVLEVGPGDDSCLSPAELMALSSGLDTPPGQEAPDGHAGAVFVLGSAFGSPGGAPMVRSKRGKILWERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDN5</accession>
    <name>A0ABQ5SDN5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_011640</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ATG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16128">
        <property type="entry name" value="Ubl_ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI67447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="140" mass="16120" checksum="BEB512CD9B41D372" modified="2025-10-08" version="1">MVTPRPLTFKQEHSLDKRKAEAARIKEKYPDRIPVIVEKAERSDIPDIDKKKYLVPSDLTVGQFVYVIRKRIKLSPEKAIFIFVKNVLPPTAALMSSIYEDHKDEDGFLYITYSGENTFGGSVKELAAPQVEQQQQHMQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE68</accession>
    <name>A0ABQ5SE68_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012510</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14212">
        <property type="entry name" value="PKc_YAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF17">
        <property type="entry name" value="HOMEODOMAIN INTERACTING PROTEIN KINASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="342"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="55"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="206"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="678"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="792"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="993"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1220"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1416"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1493"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1640"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1702"/>
            <end position="1722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1752"/>
            <end position="1779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1929"/>
            <end position="1979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="714"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="806"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="993"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1220"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1234"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1266"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1416"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1493"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1570"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1598"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1640"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1705"/>
            <end position="1722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1929"/>
            <end position="1938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1949"/>
            <end position="1965"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2036" mass="204980" checksum="15376057D9613608" modified="2025-10-08" version="1">MAVSTSWAYYQTPSQNGGASVIAVGGAGSNESMQDLHDQGGTEAAAVVGGAGNIEDSVMMSPRNDGSVSAGADGHYNSCRRGSNGGNQPQQGHTDPYGGHLGAIAHDDSAAVAVGEQGVARCAEYGTSSGSGRLQSHCEVMTTGGAGHAATMGGGTSLLQGYGNMGPGPVHCSGSGGSAHGGDGFVMSVAVSPSICSSGGGGRASSAMDAAVPGGSGGTADATTGTGAGGRRTGTGASAFRPYHMAGTLRHVEEAVSGGGGVIHRRPRSCALLVRATSGLVAVYRLCNDAYRYSQIANPRRVLTKPSVGVKNDGYDNENSDLVMHVNDVLVNGQPGAVLRHYVVHEMLGQGTFGQVVSCWCEERGASVAVKVIKNQPAYYHQARVEIGLLQLLNTRCDPADQHHVVRMVDFFLFRKHLCLVFEKLDVNLFELLRRNGFRGLSLSLVQLFLRQLLDALVVLRDASIIHCDIKPENILLKSPHSGDLKLIDFGSACFENRTVYSYIQSRFYRSPEVVLGCGYTVAIDVWSLGCVAAELFLGLPLFPGASEHDLLTRIVQTFGMPPQWLLESAKHADKYFNKRLTMDAPGQMVTQYVLTSQAEFEAKNNCKAPAGKRYFSHTSLPDIILVYPFKQSLGSEAVERERRARECFIDFLLGVLELDPRKRWTPRQAAAHPFITGEAFNGPFTPQPDPPRKVQQSLSYLPADPTPPVAVRSGHHHHHHHHQQHHSGLTGGAAATTPSGSAAAAAAAAAAAAAAAAAAAAQHVGSLGGPFSAAGGGGGLGTSLSTSTHPFSARTLAGGSGPSGLYGSLPAHGSDQAQQQQTQQQQQQQTFAPGSYTAGAGIGGGVGGASTAMSILQYSPAHHAQAHAQAHAIAMAALQQLSPNFTGMLGGSFAGGGAAAAAAASSLASSLPHPPSALLSGSFQTSGIAGSFQHPLLPALGSSLTPSVAGNLTNPIAPTFSGVQQQGQQHPHIQLGATPSAASFLQHHIASMAARASQHSQQQQVQQQGPGGCLGNSVSNGELYNPTLAAAAAAAASAAAIATQHQQHASAVVGSLPGGLSGNGPLLALGGVGAAASGVPLDQGTTADGLAAATAAIVAAARQQVQAAVAAVAAAAAAASAGSSALGTAPSASIGSAAAAAAAAVFGSSGAASGGNGGGGGSTLLSSSLPHSTGAGGMEPLIFSALLQQQQKQQEAVATAAAAAAAAAAAAAAAVSRSNGVSSSGTGMGQPSQQQQQQQQQLFLQQGSRHQLTHAYHTHHVHTQGFLQSPPGQNTPLSGTTGTAASFGAAHPYALTQNHPSSQQLHSHMCYAAALSGSQTHHHHHTLTAHGISSCGIPHVNSSNLQQYVSAALAAGFAVNATLPGTAAAGGNGAATLLAPSGNGAAGAAVCATLPHGSSFTSTDNTDQLFEMQTGATESGTSQQSQPGRNAASTTADSPSINQVGSPGDWDPLYSDEQLLGEESTLGHHHVSNTVSGGVGGGGGGMALQLAPGGAPAAAASGGRHGTRPRGSASYCDGLPRPESTSLAGGNGGGSGAHLQPRHAQGDGAAAAPPYRGPSAMQMTPPPGSHSSHQHLSCGQPQPGTAVGYSPQRLDSSGGGGTGGGAAVVDTGRRSAMDWAAAPAASESTDCGAATAMAGGAAHNQHQQEQQVSGGSGGFTHSFMAVQAGAYQPGVPTLVSHPLSYGNSSGAAAAAASPSDAAVRSTSGGNAVDSQPGVQSAGLQAGSIGTIAHALQAAQQHQHLQCQGWGGEQEQQLQQQQNQQQQPPQGVSGRTTLPGLLSTGSLLSRALPIGRPYGSTDGTHATAATSGMPLAAAAAAAVNAAAAVSSGGFWGMAAGLDPSSTAGGAFGTSSPYLYSAFGSLQAPLMLAGSYQPLSPHYLLSQHSQMRQGLQQQQQQQQQQLEQLAGGVGISRVFQSSGIGLSHGNTVGGSTSAQGPGVPAGGVETGSASSAGLEGLSTQAHQQDHERQQQQQATAAAAVASLEQQQQQQQQQLLYGGSLPYSGIMPPIMAALRSSYDGGVVQHQQQTRQQQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEA2</accession>
    <name>A0ABQ5SEA2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA polymerase delta small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_012170</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07387">
        <property type="entry name" value="MPP_PolD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040663">
        <property type="entry name" value="DNA_pol_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041863">
        <property type="entry name" value="PolD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18018">
        <property type="entry name" value="DNA_pol_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase delta subunit OB-fold" evidence="6">
        <location>
            <begin position="57"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="5">
        <location>
            <begin position="235"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="54210" checksum="3848A4DBFA10C517" modified="2025-10-08" version="1">MLDVEGPQSSHRLTARYDELLAPVTSKDNDPNFYRASAIYTNLDERFCAAKRTYERQYAQLYFSRLMLLKEIMRGRVQQLWPGIPVCSILEVQESMEVAVIGTIYKNMKLKPSILDEYSKDRGLKAALGASNFCSDNDSLVLEDEGARMVLSTPPALAAGTSSSAGADEPLNVQELVTGAVVAVRGVHEAGGDFLVSAVCFPGMAPNPHPPPGPALVSQQTTAVAADAAPGDKYIALASGLCLGGSKVDMLKIQLAFDFLTGNLGSPVEQQLSAQIVRLVIAGGCIGQLEALAGGGVGSGASGNVYNRRAQGAALQPVRDLDLLLAEVSAGMPVDLMPGAEDPANVALPQQPMHRCLLPASGMHRSFVRATNPHEFESDGVRFLGCSGQNIDDMAKYCSIRDRLDLMERMLTYRHMAPTAPDTLTCYPFHDKDPFILETTPHVYFVGNQPEFATRLVAGMSSSSLSAGQLVRLVAVPSFARTGTIVLVNLRTLACHPVRFDASLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEJ5</accession>
    <name>A0ABQ5SEJ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_012742</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="234"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="590" mass="62910" checksum="6E4C91C2FEEDA676" modified="2025-10-08" version="1">MGVRRLNSTPLADVDSEAYGTCVAFPSEHSKMQGGRLPALWKSLKAKLRSISTSRGANSLSSMATAAPDARSSPSVSPSAQAIVVCQERRFHAQGGPSHRLFMSTSSPEGALARQDTSLSCVNAQQTGANGVSGDSSPTGVCTSHNAFSTGAGSSFGSAPSNVTTKPIINITELTSPSTLSTAVVPHVDSYAPTQNPESDIIMEAASTPNRKLLAASPALPSSMRRKVWALEDYLISRRIFKGSSSAVYKATCRRSGMPVALKVYFLERVPTNVVHMLKREIEIHIQLLHRHVVRLYAAFLDEGDRVVLVQEYAAQGDLYGVLQRMGGRMPIEQVANGVMRPFLEALAYMHSRGICHRDIKPENIFFTQSWRLLVADYGVSIDLNHERAVTRAGTEGYMAPEVERCPLKTEPHENKDSPKYAYSTAVDIWAVGVLAYELMVGFPPVVMSAGPGNPVATLGVSDDNSVNVFVRSHMNAASLHFPASVPDVARAFVLAALASDPQERPTAAQLLQHPWLQPKSVLERTAAVPEALPAPGGRSAGIPEGGLRCGDVENRFIPNAAAGSIAVAAGQRPLMKAGSPAASALCVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGK5</accession>
    <name>A0ABQ5SGK5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aminodeoxychorismate synthase</fullName>
            <ecNumber evidence="4">2.6.1.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Para-aminobenzoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">p-aminobenzoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_013606</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11672"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58406"/>
            <dbReference type="EC" id="2.6.1.85"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the anthranilate synthase component I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.85" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF18">
        <property type="entry name" value="AMINODEOXYCHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="11">
        <location>
            <begin position="4"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="11">
        <location>
            <begin position="207"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="13">
        <location>
            <begin position="457"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="12">
        <location>
            <begin position="773"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="363"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="628"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI69063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1045" mass="111320" checksum="C842D34D10F4CEA6" modified="2025-10-08" version="1" fragment="single">MRTLLIDNYDSYTYNLYQLIAEVNGELPVVYHNDKITLEQISSMVKAGTVQSIVISPGPGTPHKSSDVGVCIDLLQQLPEVPILGICLGHQALALAHGASVITAPEPVHGRLSRLRHKGHALYKDIPSGDGFEVVRYHSLVVDERSLPECLEAVCWTTGGHSAVKLARDTAAEPYAANPVESHVGERPDVYHPDQQLQQHEGTSGCGCLLMGIVHRSRPHFGVQFHPESVATRYGIALLRNFRDLVAEHIKRRFGSEAAQLPPLIHLRDIVGPPGRTTLSGLPPWPAPEHQCGATLRLLWSRLAGLLHRLPGGSQALFELLYGWANDTFWLDSAAADRGRFSYMGGRGGQLWRKLTYKLPLPTSTAAAHQPTSQPASQFEAQPSRGDGVNDRRQLPSLDSEDQPTGAPHNTDNDGASSSSTAGACSFSYELSPTMGPSSCTTASTAAAQSAPHPACTTALGGGTVSVEKPDGYVVRMAAPSGFLGFLEGLLERQRLLVEPEAAAQLPFNFWGGLVGYLGYELKAECGGSNVHTSTTPDAVMYLADRVVAVDNATGDVFLLALYDNWSCGHAAVDAEPEGAGRSPGSAGCTCTIAGTLKVEAQAWLEETERILEAAVEQLKANSRRETASASAAAPSPSSASTAPSYPSGWQCDRRLRPEASCDAASSFVHLPGACDRVGPETEGVCDICAELRVTDQLDAPGPCRPPPSQHQWLPQMSASTSTSLRLSAAVGNGCTAGQEGLGLQVTMDAAAACPSSALLDGKVVPFVLAHSRRAYLRNIAACHAALHAGESYEVCLTTGLTRTAAPNPAQLYRTLRQLNPAPYAAWMQCGAEGPTICCSSPERFLRGDRCGLLEARPIKGTAARVPSDPVADAASAAELARSEKERAENLMIVDLLRNDLGRVCQVGTVHVPGLMEIESYATVHQMVSTVRGVRRAGMSIIDCIRAAFPGGSMTGAPKVRTMQIIDVLEDRPRGIYSGSIGFISFNDTFDLNIVIRTAIIEDLAPDSARKPDAVAPGRTISTAEAHLVGTQQHTPMRRMMTIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGR8</accession>
    <name>A0ABQ5SGR8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">2-dehydropantoate 2-reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050838">
        <property type="entry name" value="Ketopantoate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765:SF2">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="4">
        <location>
            <begin position="7"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="5">
        <location>
            <begin position="297"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="46696" checksum="CF5EE79B0F131D9F" modified="2025-10-08" version="1">MASSQVWHVLGGGSLGLLCAFHLRTVGFAVNLILRSSSAVTAFKQSGSCVGLLESWRKDSVPSWRRSPPLQATSIPCIVNTASDNNSHVFCGEFLSKGLGATRINRLIVATKAQDTVLALRNLLPYMDPGCRCILLQNGVLAVAEELQQTLGEQLGWTTRSPDLHPQGRAAMYADVAGIKDVAAGKMRLYVASVTHGCYREAPPQQDIQRLPSDSLAFGLVHAGLGSITVGVLGPFLASTSAPLLTGKPSITPEHSPPQRFDTVHGDDMAFLQDLAADLPDLNIHPEADSSVLLHGLLAKLAANCAINPLTVLLGCCNGTVTELPQTRGIILDICRELIAIFGPTPFAPPGLKSGDRELAPLDCTKEESEKALYDWVLAVAAATANNRSSMLQDIAAGRATEVDYLNGWVLRAAAARGLQAPVNATLHALVKAREKLVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH72</accession>
    <name>A0ABQ5SH72_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sas10 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018972">
        <property type="entry name" value="Sas10_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF9">
        <property type="entry name" value="NEUROGUIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09368">
        <property type="entry name" value="Sas10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sas10 C-terminal" evidence="3">
        <location>
            <begin position="703"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="111"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="418"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="624"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="678"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="638"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="717"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="774" mass="84349" checksum="B546D3FF9303B874" modified="2025-10-08" version="1">MVKPQRRPPLKQPASKGGSLREEEFIDTDSDDDAYKKDKISLNMSDDDEPSDDDGLDVEGVYDLDEEDEDEEGEDEDEEDEDEDDDAIIRDAIKRGGRIAELAKQAKKLGQKLKLQHGEDDDEEEEEGVKASKLGSKNEEADERLWGASKRAYYNADTAEAASDDELLKEEEQEALALQRARAAKLAAADFGLEEVEEEDEGEDEEVETMGGLASRATAGGREGAKVEVEAVARDLSALGDEARLSALMADAPELMSLLQDLQESLAEVRHRVSPLLTELRDGGLGTTEGLSYLEAKHLLLLSYCTHIVFYLLLKAEGRPVRDHPVIGRLVELRAYLEKIRPIDKQLSYQIDKLLKAAQIAAAAEPQTVAGRTNAAHEGDEAAGPGPGSAAAAGLDADVLKYGPRPDALVPKVKVRDGADENGEGAAGELAGVYRPPKINPTSMEMDGQRAPGGASLSAQEQRRLKELKAKAKRSDTLRALAAELAGAPEEERAVVAGMDSLAALRTRAKLEARVAVEEDNFIRVPLSKDEAKRLRQQRRAGMSGAGMLEDFGDEVADLVDMTRGVAGTTGAAAARGNALNDLFARQKVSQRFGADHPSLSRRAHGGDEDLPLREPLHERRAKFDASAARRAVKANDDGLDGAEEYDLGGGVKGTRKRQFSGEEDEFYAQAKADAAARKKVRAERHRAPALEPPAPDVTTAAPRRITNEIQRNRGLTPHRRRDQKNPRKKNRMKFEKAMVRIKGAVQPVRQREDVAYGGEATGIKSKVAKSRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHK7</accession>
    <name>A0ABQ5SHK7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="29037" checksum="1AB1C7E4B3E3DD0D" modified="2025-10-08" version="1">MSETGSARVTQEYSDDGRRQPPATPIQSVPGKTGRINYGLNETVVRVPVLDKVKEARKATIESKPDVLGLRGPVWHDSVALNPGKHHHNFSGNVLSNTLSPSLINNKDVRKLAGTTACKADKDASTLDRSRSPNNNPGSWNASIAVPDNLEKQRQLDAVTHSSLAATRRKSPPANYIDPISRQTAISETIRAMKANSGSDMQELYDRYGREGGEAMAAILRMQPQESKPRIRPTRADLDCVRALDDFLPGQEKVEEEGEAVPLGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHM1</accession>
    <name>A0ABQ5SHM1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="614" mass="64559" checksum="A045C4335485B5AC" modified="2025-10-08" version="1">MEWGREVLGCTLILSSPLAACYGTKLLARLGLELEETRRVFSLRQSLASCAGLRARGPEAQNENAERSQEFTGENCFQYNNAAFVCQSCDDYGADTAECEDSEELDFCILTPQQALGALGEHAPQYAKIFLNGYTFVADRSEPGTVMSVDEYADRMHVSAASRRHSAGGSCLATSSVTESQTCGAASPGFTPGRSPNDQLSTCSDSSSLRCRSSCARDFSTSAPTFSERSSFELSLLSYQSSLGLARTTLESDTYYDTMPHTYPVLQNGFDLDATQAHVEQGMTAAAEAMTQEVQVQHRTGRNDCAAPVTPREFAGPQRPVLSPFTDSGACHAETSPSSPVDTWRSGGRAQCAGVHGAEHVGGVKAYQGSGCSSSQSRSVGALHKCAAPGGTLGAAEQKGSKRPWVPSGVRLVPSPLLQTPAPRASVPLSSSHTSRMAVNVKKDANTACSWEAASAPHSPLQPTCSRSCGSRSVGGSPSSIAISPAGLCTAPALPLPDGTTLESAVPPALRYYLAQTRAAGNQDVQGVEAGRRAPEMAARCQSSGCDSTGQGVGSADISAGQPGFVRATVEQLNTQRLVCCHVTPYAAAKTCRKPRPLVQRVPVSKSEVLLNYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI49</accession>
    <name>A0ABQ5SI49_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_014045</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF5">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22061">
        <property type="entry name" value="CSN7_HB_subdom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="5">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="253"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="268"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI69434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="282" mass="30524" checksum="A5DBC6493BCBF7A7" modified="2025-10-08" version="1">MDQEVKIQQYVLLAKGIRGRGLTEIIAKATADPGVFGFGELMDVPSIKELQGSDLAPHFALLQLFAYGTWSDYQANATGFPALTEQQVLKLKQLTVVSLAASQKVLPYSQLQASLQIGTVRELEDFLINHCFYTGIIIAGKLDQKQACLQVHDVVGRDVRPEQLPELVQRMANWIAAGDELLRAIEVRVAYATATSDAARQHREELEARVEDVKRAIKAEARSAEGIGGLEDATLLDLMDEDRIGGMGAVGLGLGGLEGPGPSGSGTRGERAPGRPKRKYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI94</accession>
    <name>A0ABQ5SI94_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018881">
        <property type="entry name" value="C2orf69_mit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31296:SF1">
        <property type="entry name" value="MITOCHONDRIAL PROTEIN C2ORF69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31296">
        <property type="entry name" value="UPF0565 PROTEIN C2ORF69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10561">
        <property type="entry name" value="C2orf69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="335" mass="36297" checksum="B41B23D595175BE8" modified="2025-10-08" version="1" fragment="single">IKMHSNSFHAHTCGAQAVFVQGEAGTSEVRFARGCGDQAVVFFEGDQILDEDTRDLLGSELWSPESHLATLRSQAGSETSVFVVMPTTVAVGGQGCYGNFLAAMTAAGEPLGFKPQDYRAATHLSAILSACGYCWGCQDDHMAKDSGGATRSDSALAGIVLVGFSKGAVVINQLLAEMAWCEDSPEEGAEASSALGVKTEESEPRPYRDKWRPPSATSQKLPAVAALLDSIHAVHFFDAGLNCRGVHLTDLGVTALLGKRHSRRALVVHLHGTPRQWADRRRPWIAQERDRFLGLLGEFGVPAFLHHYFEGQMPSLRQHFDLIRRARLVVDGCSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJE0</accession>
    <name>A0ABQ5SJE0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031367">
        <property type="entry name" value="CCDC24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28601">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28601:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15669">
        <property type="entry name" value="CCDC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="474"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="543"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="647"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="771" mass="77285" checksum="D0964F3730D31F35" modified="2025-10-08" version="1">MDGQPYPTSNAQQLRVVKQLLWDVISLHLYPAERDEIRRALGNSLLDDNETLFAEAGALADILGDVQLNTSALLARHQLCSNPQRSMVESEIRLLIDRLHASAAVSPFGSAVVKERDPESLLPRQGKRDKAVLEYVTTVAQIVESSGRPPSAGLPPRPPSTAVSSSSRPGTSSRPPTALSASASASISLPSPLSRPGTASPTLPGSRPSTASSAASSAATRYTDAPSVVAGVADKLNVSSIDAVRETLRTALLDERAALLEDVEYLQGLLEAEADLQVRAAAPPPSLTELKDYSSRLAVVVADEEARVEHEVRVSAMFAAAERQQSKPGRLRGMVDASRRPTSGGGSPGSVVKAGPPLETAPASVHPPLRRISASASGGTQTFVVHGGGDETILQQHRQPSELSTGLAEAMATSADEGNGSLGPASTSSRAAPLAAVRRPGSGGGSSRGSAASVAALTGIPPLRSVAPLPPTQASPSACANGSPAAAARGSNTNASATPSAAADSNGQGSSPAGPSGRLLHAPSPGRLRPAAATAIPQAQVHSQAPQPQQISPSQPYTHHDKSAPVLTRPNLAHGIAAPPSTAPGLSSSPAKAGTGALLLAAPATISNVGSVNTVAQTGLRPVLSRSGSAGRGATKGMAGAPASVPVNGSISSTPRAGSRASSTPISGDSPAGKAASGGISGSTVIARLNATGAAATESHGMAVQPRALLGNKQSVVEVLDEEVDKVKGGVAAILERYNIRASTPSAPGAAATAAAAAVVAAPPASSRGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJV5</accession>
    <name>A0ABQ5SJV5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">1,4-alpha-glucan branching enzyme</fullName>
            <ecNumber evidence="4">2.4.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015107</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11321">
        <property type="entry name" value="AmyAc_bac_euk_BE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02854">
        <property type="entry name" value="E_set_GBE_euk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="7">
        <location>
            <begin position="341"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="864" mass="98590" checksum="26C452BD8185577C" modified="2025-10-08" version="1">MHQFARKHISIGPHSNTSLIAREARSFGAVVGSNRSTLCTAALPYAFRADPTSALEVLQEENTLLKTTVSEAKSAIAGLEAQLKAAGIPLPPPEIVPGIMPTQPEDFWSPELEVPYDYVHRDQYGSISPVPGHDGTECFTWDPALWNFAEHFRYRWRRLQEIREAIDDNEGGLDNFTKSYTRFGLNRGEHLGRRGIWYREWAPGAKALALVGDFNNWQPRDHHWAFKNTYGVWELFLPDGPDGSPALPHRAKVKCRLETGDGRWVERIPAWITWATQAWNEIQFNGVYWDPPETGAPGEIASDKKYVFRYPRPPRPRTLRIYECHVGMSSQEPKVNSYLEFRRDVLPRVRALGYNAIQIMAVQEHAYYGSFGYHVTNFFAPSSRCGTPEELKALIDEAHRMGLMVLMDIVHSHASKNTNDGINMFDGTDAMYFHGGPRGYHWMWDSRCFDYGNWETLRFLLSNCRYWMDEYKFDGFRFDGVTSMMYYHHGLSYTFTGNYEEYFGLNTDVDAVVYLMLVNNLLHDLFPNCVTVGEDVSGMPAFCRPWQEGGVGFDYRLQMAIADKWIEMLRSHDDCSWDMGTITHTLTNRRYAEACVSYAESHDQALVGDKTIAFWLMDKEMYDFMSLPGLGPASAIIDRGIALHKMIRLVTITLGGESYLNFMGNEFGHPEWIDFPRDNSYDPSTGRLIPGNGGSFEKCRRRWDLADSPTLKYRWLNAFDRAMMHLEKAFGFQCAPHQWISRSDSADKLIVCERGDLVFVFNFHPTNSYTDYRVGCNASGPYKVVLSSDEEIFGGYRNATKEANVTFVATPTPHDSRPFSFMVYAPSRTVVVYAPAAWVDPEADRKPHGIPGLAVRNLGPYYAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKB0</accession>
    <name>A0ABQ5SKB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19335" checksum="4D63177DACE54677" modified="2025-10-08" version="1">MAEPSSQDSTSTPVCREPNVIQHMPRQRAKPPMMAQTEAALRRAAEIAEKPPVHLSREQVSRAYDILEQQRIGPYAVKYGCIGSVGLIAYTYLQGFYHLAHKNRDAWMGSFRHRILILGPIIAMYATANDVIPLPYKERIEAALNRPSISMYDIATAKETVKKAQTSPLSAQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKB4</accession>
    <name>A0ABQ5SKB4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055336">
        <property type="entry name" value="At4g00755-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39741">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39741:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="689"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="809" mass="87856" checksum="2D81839FA5DB801E" modified="2025-10-08" version="1">MAAEYPPRDRLSLRAYIRARMRDRGLLEFADDDEVARSRSEMVYMNCGQSWCEGALSRTELELEALLATTTDCPMQSIGNTVLDNGEFWSSTGTSTCKADEALLYRLRQPLSSIAYVSVAVYRAFYQHGDPLYPPRKVSFLTGPSPNQLYPASPIYPVRLTDDAQVFALSPTAAVSQYLMVRMHGRRQRQWEDLQFYIAIRHVAAYGDLVDVSSSIQMMRLARSRGLAWPCTRGLMSAMATLGTVWEHPQEQGAPGGARGRTRPGDAEGACLWPLPLRRRSSTGAMPDQALSEDVFTAGPKLSSEARRVYVSVAMRRGPALPSLALEPDIPVRTGGLRGPAHCPVLPSGPTYSELSLAVQHSGVLRALPHLVKIPWPEILSVTTRRRMRQQHLHEKEQKQQLPVLAVKVDNPNRRSPSLRRHSDFEEGLYGRLPTRRHGEDALHYLNRERSGISGATRGIARLAGNAARRGIGSLQRLHEWVVAAAKGTSAAVANDDTDPATNMGRGEAGAQRAEELPGDVRGRFFSSMNTAGGGSHNGAQLLGTISTGRDLSSSDPRRLQHPDDEDQQEDVDVAPEWLWEVFTGSDSDEPFATEMPATSGPSGDTRVGAMARSAADASGNTAEREAAELRALVSGQNSHLGLLTNNSMITNARDSNLQLMVQSIQAYGDADETDGAAAAFTSGGCNALRHARPQQPLPPPKLRRRGDDGHEGSSAPGKVAVDGGDGGEAQLRRRQRHLRAARRAMQPALYRPYKDTALFIALDVWAGARMYSSCGSSSARRQAAAAAAGGGGDGAGCWLVSHYDHEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKJ3</accession>
    <name>A0ABQ5SKJ3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="589" mass="67176" checksum="5FA787E7CCE1079F" modified="2025-10-08" version="1">MHADTPANRKLSLWNSHTSATECGHCLLKGVSGEGGGMYFPGYAVDADVNCIFDGVIPDMENAPLSARCGDPRIMVSHEMHLKRAALAMKDPQWTGIVGCKGLSPIIRELDYCNYNDVFVVPVAHAALFGVIKRFWNTILGKLGRHVMDYTILGPQRKIMAQRASHIITTNDLNRPYRDIVKHRGTWTMEDWLHWLDSYSVYILHEEDSLQSSTPLMPVVQRVVDGITKSFNIKVMWNNLRTALLHYFRYDPSDFSADANNHAARLLKSFSIAAEHVFGIQFCTYNLHILVCRLREQENARGHVAFATEFWVERGMQMMKSIVKYRSPTVPELVLAGGYLASLWLTHLKAAHPETFRTFDEWVPGYRADNIQLNPSKRDVFLDEEDSEGSQLLGQGYIADAEQRSHILGGILRTEETEDFMARTNLLPSVLQDSDIYIFKRALKRRDEVVLSVDYARATKRQSYHFKCKYGPGGMVSESDPRVSIFIGEILFFALIRPRCDVAPVKVAVCNLHRAEVLRPKELYRVKDYSRRDATVNEVRQEILLNYFPKEVGMIDSKVIRCRVNNELSYFLTYVTFSQLPTNDGGEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL43</accession>
    <name>A0ABQ5SL43_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">plastoquinol--plastocyanin reductase</fullName>
            <ecNumber evidence="3">7.1.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">VaNZ11_015735</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2 oxidized [plastocyanin] + a plastoquinol + 2 H(+)(in) = 2 reduced [plastocyanin] + a plastoquinone + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:22148"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="Rhea" id="RHEA-COMP:10039"/>
            <dbReference type="Rhea" id="RHEA-COMP:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
            <dbReference type="EC" id="7.1.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rieske iron-sulfur protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.6" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03471">
        <property type="entry name" value="Rieske_cytochrome_b6f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.700">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005805">
        <property type="entry name" value="Rieske_Fe-S_prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057415">
        <property type="entry name" value="TM_PetC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045928">
        <property type="entry name" value="Cytb6fFeSPetC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010001">
        <property type="entry name" value="PRK13474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25471">
        <property type="entry name" value="TM_PetC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00162">
        <property type="entry name" value="RIESKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rieske" evidence="17">
        <location>
            <begin position="141"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GLI70712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="255" mass="27022" checksum="F25697C2832F9323" modified="2025-10-08" version="1" fragment="single">TPYRVPYKDISWALPSHIERGDITHPSALDICSGRSVVVLSLSITTVATMAMITSRRAAAPCKAQAARRSRVMPVVRAAAVSSEVPDINKRNIMNLILLGGTSLPVGSLALGYGAFFVPPSSGGGSGGQAAKDALGNDIKVDSWLATHQKGDRSLSQGLKGDPTYLIVTSDGTIEKYGLNAVCTHLGCVVPWVAAENKFKCPCHGSQYNAEGKVVRGPAPLSLALAHCDVEESGLVTFSTWTETDFRTGLEPWWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLQ0</accession>
    <name>A0ABQ5SLQ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_015358</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd21546">
        <property type="entry name" value="SPOC_FPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="1028"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="241"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="306"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="613"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="687"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1155"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1225"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1333"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="722"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="735"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1225"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1405"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1515"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1559"/>
            <end position="1582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI70445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1736" mass="176737" checksum="37790ED33684BC8C" modified="2025-10-08" version="1">MSGDSALEHSSTNGSKRRRTITGDGDAPAAKKSFTDAEIAAEVALSLHIREQRRAEVRSIYLNYGTLLKGSETGSEALAFQGLLDCANGSLGARRLAARLVPRFLPRFPSHAEQAFRLLSSLYGDGQPPRAPGGLNGYVTSSVTTAEGTPRNAGPAAVITDGCDDSVDLEEAMRRDALRGLGNVLEAAAAARQIERNVPVVMELVLFLMRHMRRLQASSSGSGVGRGSGGGASDTALKSLKAAGSAPPPKPPQSPKAPETQLPKLPEPQQPKQLSSDQNVSVNAMLGHGLKHRYIRRNAEAAANAEANGGGPWRARSANRCPSDDEGGRSGRAAQHSPTPPPASVAPFSSGAQTHEGTDRCGKVGGGDETENGPGAGKGNSAPAGVPIDLAATQDPDSDVAVIRSLLCRTFAMFPRVVLTASLQPFRLPTKQRGHEDCEVCGQLLELLLQPAPASEFTKSVAATVVGLTTELTEAVNEGGGGGSSGMGNYRGCNIDGEGAAGSNAAAGDAAALGPGRGPSSADGTACSSCLAARVLAQLPETQTWFRQLATAIMQGKTQHSLPSSVQGLLERLLQYCTASVVPAAAALGKVSPRASPHGAVAATGGTGVPCGGSVNLTSTRQGPVKPDAGPASTAAHSMVGSDDNSRSVPMDLDHSISTAVIRPEGSGAVTTPNVAGAGAAAAAALAGPQDTAATPAPPPLLPPQHSSYPQHHHLQNDGLLAGQAAGPATVPAGAVPPQPHPLPPPPPLPSLSLYLNRGSSGLRGGPVCRAEACLYIGGLPPGLTEAALFAELSRLGNVESVHPFDQQQVLQQHPVHGCLSGPQVPLGEEVYVVFMSLRDAAICYEAVARTCLFGGTRPLVVEFCSAFPADSPAARRCVAAAAMAAAGGGGTSSTASEFVWVSLSASAAVTPESVVTALQAASIPVPQQILQVAGRAPGMLLHMASAASVPQVAACLQAHFVHPPLPPPPSVTTSAATSSNAGISLPPPPVMAAAQTVPSGAGGHYSTPLYMQQASAGGSSVDSCNFRTIWIGQLHESVREEELLAACRVHGDVVGHRLLRTSHCAFVDFASQAGAEAAKRALHGTRLGPQHIRVEWKLDTSLSSRAPLTLRHGSAPLPGNASGNHVPFGLGSGAPGLVTSPSCAPGIGLPGSAGHSLAGQGLPQPSPAAAGMGMMGQHMTPQAMAAANVAAAAAAAAARGTFAGSCNGTDRWAPVVMPLAPTNAATPSPAAAPAGNTGPVASGGSGFLTSNQVPLQSPVQSQQHLPVGPFGTGVMNATVAVAARPTAPMLIPHQQILAGLGGGVMLAHGMVLQHQGSSFTTQQQAQLLHTHATAPSGSGGGALTHAPLPSQPHTPVQLPVHLQPMGGYPGPPPLQQPQGPLGLHGHSTAPMIVSHQHALGIGGPSPQHPTQQQHPQHAHGHPPGQLQQQRPSDWPQHGQGVTAGGTHSTASPGLPGPGGTLAQLQRFELGTGPGMPLPNSPPMLLDGMTAVTASGGGAPVAGPPPQALPHAHSQHTLPQAKQGPAQQHGSAPIHIQQPHLHTHAHTPHQQLPPPPPLQQLQQQQSHHLFNQQQQQLTSPPSVTWQGGLAKSGSLVCTLQCITGGANAAGATPGEREPVTWPATLDVKLRVDLSYVIHSLYSHTTPHARAMRRLVTAGGADQRTKLSDFMTYLLDKNRAGVIKLEAAAGLPPRTLYLVPPSEQVCAALGAEWTAGEPFLLALVVPTASGTTGANKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLV4</accession>
    <name>A0ABQ5SLV4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_016087</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="2 6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="6" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="4 6" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="255" mass="27330" checksum="A4313EB9F7EED791" modified="2025-10-08" version="1">MAFAVTFSRKALQVSAKATGKKGTSKTAAKPAPSGIEWYGPNRAKWLGPYSENATPAYLTGEFPGDYGWDTAGLSADPETFKRYRELELIHARWAMLGALGCLTPELLSKNGVQFGEAVWFKAGAQIFQEGGLDYLGNPSLVHAQNIVATLAFQVILMGLVEGYRVNGGPAGEGLDPLYPGESFDPLGLADDPDTFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPVENLNDHLANPAVNNAFAFATKFTPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM98</accession>
    <name>A0ABQ5SM98_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000102">
        <property type="protein sequence ID" value="GLI71117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="244"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="878"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="475"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="581"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="733"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="802"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="882"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="898" mass="96704" checksum="BD3DC8D9A45C4EBD" modified="2025-10-08" version="1" fragment="single">MQRDDGDALGVGAFSVDGRNRSVRGGIRNTGPAPADPRTASQLLKLHRLVESLVIRELERGESPNVAQDQGQDIPMRSQNRQPQQLTRSTANGGGISGGVSVQPRPAEASRGRKLARHAHYNDSTITSLDAMSMPYSSGGSDGVISGSTDVLGTDAGRRMYGTGTASADQPRPGRPGFRSTPSGALEEGVHNQVAALQSQLALLGQQLDAAYRAAETGEVASGAAPGLGAGAGSGVGLLRGQGKKNLPGLGGETQGSVSSADAAATPLTGSGSGRPLHSVSQPGAVTTLASLLQHQRRQQQLPPAGKDGQSSAASGEGAAGRGGTQAQAAAAAASGSSFFGPGSHGPSSSSRHDLPQVTLEGHGREASSKEDERLRNWIQELRLERRRLEDRLLAGDPSGGAERAQEIREDLEQQILSLSRQRDHLALQLDELGVTQTTNSQLAAQVDALEAALAAEVECVVRLKEVRPILEGKLAEVSASLESLRRQHEAAMEALRQERDAALSKARRNAKRTGELEAAVEAAHEEVRRLQDEVRAHVMSLAEMRASGEVLQSASQQMQVNMEAQAKETATRLATVQSQLEAGQSDCAILRRQLEASQREAIMLRDQLKDSQARGEMHSAERARLGTSLAAEQKAVAAARQQVESLTAQLKAGDEARADLAKAASAQLEAVKRQHAADMAATQKQYESQLAVAEKQYLLLQRQYDAGLASSNQATEVAAALRRERDALWAETQNLKTELDEIRTERNELSSKLTSLRVEVRNTMEEVTRERDTKLGAAKSQHEVALRELRAEADQRISTMRQDYETKLANVLDAAEKLRAEAQEAQRQAEKYRTAAGHLKEQVRDLLLSRDAAERELASERAFNEELNRILTTLREEQHTQHQQQTMLQQHQQRIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMM4</accession>
    <name>A0ABQ5SMM4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="364" mass="39156" checksum="875C7E06F3BF8B43" modified="2025-10-08" version="1">MKQFALISLACAIWLANKRRRNSTFSNTNEPSPAVNRSSTYVIPRAEAQDNIPAAQVASAPAGARTQPAPSTSSTDTMLQWTFLSLMSVALYLTAAKGSWDVFAVLVAAAVLQLSGQRMVWLGSDSEGSAESPGKRACLSVGTVTAQRPISGAAALKATAPTRLLLPDQRLGGVWVKDPTRSDSMDEATSAMRLNGIVRTAIRLIRGLELDPEALKAGRFDMAIFSVITFFKVRESYLLDGTVASYNRRDLRRGKHAAFVTVQPDGSIVLSVSWGEPLAGTGTDHFAIASAATEGLGPERGTADQPSMVGADGNVLVVTSTFRLVETGRTVTYRTTYVRAGRGRHHQQQQVHDNDISRVPQQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNC1</accession>
    <name>A0ABQ5SNC1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_015948</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23793">
        <property type="entry name" value="UBCc_UBE2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="8"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="155" mass="17202" checksum="1AA1BD27EB7A6DB2" modified="2025-10-08" version="1">MKGSQHSSSAKRIQKELAEISLDPPCNCSAGPKGDNIYEWVSTIVGPSGSPYAGGVFFLDITFPHDYPFKPPKIVFRTRIYHCNVNSQGQICLDILKDQWSPALTISKVLLSVCSLLTDCNPNDPLVGSIAQQYLTDREAHDRTAEEWTKRYAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNE1</accession>
    <name>A0ABQ5SNE1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016675</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF12">
        <property type="entry name" value="AT HOOK TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046730599" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="34"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="314"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="470" mass="49742" checksum="105BA34CF4FFE84F" modified="2025-10-08" version="1">MALKSVLLGLLCALAGVAVTGAQILDGGSSLDAFPFCRCETNVTKSPYRVVYNGFLVGYLKQDWTCFRIYLDQGAATSCPSTAPGLNCCNSTLYKVEFNVDASCKGAINRALLFKDMTNVTDGVDLKEKVITVGIETVAANVTGVLRVVKGLAMPQSVLAKYPEGLPICLNQVPTKCSLKKTFLYDSNGGFKYAVFDSSKSCCPTSLTNIINFLVPPASPDNTPLAPGFDRPSPNRPDPPPLSRPPSPSPPPSPPPSPKPPKPSSPPPPPPPPPPPPPPPTPPPPPPPPPPPPPPPPPPSPKPPSPPPPPPGTFPYCECKSFDPADYPWRAVFYNSTLTKIGTRVCMKLYVDLAAAARCNKALGCCEERLQKVEIYANPACKGSITPFTLTGVTGSKSSFLWHTTFPTLKFTNLNLPYEAGKDGAYLCFTLKGPSCTAVTQLCNIADGCVAATFAPPGNSCCPINPMPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP78</accession>
    <name>A0ABQ5SP78_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Argonaute-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_017214</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the argonaute family. Ago subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000125">
        <property type="protein sequence ID" value="GLI71820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="PAZ" evidence="4">
        <location>
            <begin position="389"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="5">
        <location>
            <begin position="688"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="53"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="53"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI71820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1049" mass="110566" checksum="F1B02022D81D2684" modified="2025-10-08" version="1">MSGRGRGGGGGGGYGGGGGGPGGGGSRPGSAYGGGGGGGGGYGGGGGGRGGGGRGGGFDGGRGGGGGFDGGRGGGGGRGGGFDGGRGGGGYGGGGRGGGGGGVVSPQEGAARLEAVLRGTNTIKAPVNVEVNADGRPTAITRRPNHGTVGKAVNLLTNYFKLQMLPGFPQVAYHYDVSIKSTDATSGVRGGATRGVRAYPAPPATEMASLTISGEPAASEGEDLPPRLANRILSACAKEHKWPQGAWRYDGRKNLFLPNQGIPPEVRNWKVAVPPRQGDRNDRPKSYEVTTKMVNAVDLTNLQSYLARQQQVAPRDAMQVLDIVIRHAFAVDPLCSVVGRGFFYPGDGVSSLSGGAELWTGFQQSFKLVDTGLMLNLDSAFAAFMSERPLPDLLAEMCNARDLSRVDINRLRAASRSLAGFKVAYPIKGGFLRRKALIGLSEAGASSTKFFNDKENKEMTVADYFKATGRPLRFPALPCANVGDRRRPVYIPLELCTVVAGQRRMKLDAKQSANMIAAAKEDPGMKHQSVSRQAARVTETLVKAGTESCWGLKLNTELLRLNGRVLPAPVLAYGDPQAFDVGSSGSWNLRNVKFVDPRKLDSWAVVVLIPREEVDFEGEYSLWDFLIDMCDGMAKCGMAVVDPVRRNSQVSPPVEFQKEGNKQARGIEATMRAAADGAQRKYGKPAQLLLVVLPEPLTDEYREIKRVSDMELGIPSQVVAGSKAKVGPKAGNKGGGPQYCANVAMKINNKLGGVNVALSGGLRYLPVLGGQGAIPFMVMGADVTHPTGAAARADARDPSVAAVVASLDHTLGRWGSRVLLQTGRQEVITGMATATKELLLEFYRSNRNTKPQRLVMYRDGVSEGQFDQVLAEEYMAIRKACSELEANYRPAITFIVVQKRHNTRLLPADNAGSDSKGNVLPGTVVDKGIVSPDAFDFYLNSHAGLQGTNKPAHYHVLIDEIGFGADGVQLLTYWLCYLYQRTTKSVSYCPPAYYADRAAFRGRTLLAASSSASDTASEAGSVRAGQGGASAPAKFAGISPQLSNVLYFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPJ5</accession>
    <name>A0ABQ5SPJ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GAG-pre-integrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000106">
        <property type="protein sequence ID" value="GLI71338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAG-pre-integrase" evidence="1">
        <location>
            <begin position="114"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="165" mass="17666" checksum="3FD2E78B5AD19E78" modified="2025-10-08" version="1" fragment="single">SEKFEILSVNGGIIRPTAVGTLRLIPGVNMHARVTVEEVYLVPDSFANVLSVAALDKQGGSVCFEDGQVTMRKGGEKILTGQRLGDLYALSCFSIPRSASRLRYTQPFGGVAHGKSASESMDLWHRRYGHLGLGNLERLVGEGMVSGVRFAKEQVKGAGDAGVCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPL6</accession>
    <name>A0ABQ5SPL6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015977</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="188"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="477"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="579"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="727"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="777"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="907"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="981"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1030"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1191"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1319"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1547"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1744"/>
            <end position="1765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1814"/>
            <end position="1833"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1406"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="587"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="626"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="648"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="662"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="677"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="727"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="791"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="991"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1195"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1321"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1818"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2112" mass="214102" checksum="F1F20F4F8452DF3B" modified="2025-10-08" version="1" fragment="single">QQQQQRSPMVGRSATLRKLRRAVDLIRIAMLLKAGSSSSGNSKREGALYASSPQLRPPPELSGAESLKAELSNMDLRPKIKRGRHRRTSVDGFLRDLLGRNFAQHQWQHQTQRRSLDRDSTGGQMMGSRYFDDDSDEEDTAGQSQPTKEPPYEIGLPVPPPPRASFEYLIAQDGTYLPWSAQSSRAASFESATSANRSNQLTGNETNRQSPTHRSSDSALSGCGGGLKEVVQGNRSCGGGAGAGSTSCDGGVSGDGGGGARRSAVPPSPPAPSMPPPAPPLPPPSQLQPALPMMPQLPPLSSAPPLKSGGLTAAMSYSTHQPSRFAPKGIGDSPRPVSPAGRTPQLSPSPWSTSPTAAATAAMTLPPSPAPAVPTELPPLSLAGNNFEKDGRDSVNAVVSGGGDVSVGGGQRPHTPVTPPSRSSEDFRKTSVQRRLKQSAFMTSGDQQVSVEVTTSNPITSDLELDDLIQAISRSSSNFPIGHADGSRQGTGTGSGSCPSESPRRGAAAGGGGCGGSAPHRMPSGSDGSIGDAAGATLQVIVRRGSCDQLSLGSSKLSMAVEVQPGAVEEICPLETLTPSPVRLQPSELQPQSSTPLHMPQPSQLASNPLAQPPSQQQRQRRHQNAPSALTAAAAARGGPLPGGEDGQALAALSQRAQSPTPSSPSLPPPPALQQPVRTSVTARENPSTGLAHVYAAENVRVVVGGGNQGSGGGGCGAGSPSVDGGIGVGGNADTGNDGGEGGGIGGGIGGGGGGGGGGGGVAAHPVLQRMQTFWEAPEGDIGDSPPPPSAQSLQQQQARSVGAGRGRGGVSKTVQVLLSKLNALPPSLRAEIVQQLDVPFGVRPLLPLPPPSLAAPAPVTAAGAENGAVSSPFATATGAAVAPVGASADAAPMAVAAAANAVVASSKTSGGSNGRPDLQQQQKFSDGPGLVSEPGPGAVPIGLQVGPICAVPLLAINSRHRHPLPAELFSEISATAAQQYGASPPYAPDSTRSPALTATASPSGGLLAMGKTAQLAGTAGMPLPAVHLATVTPGGSPPHGLASSNDSSLEADAVLRSRATRRSLQPSEAFLSGKMDCTSFNGAGPVTHGSVHQPRVVAALQAAGLLGNLSAPAPVLSSDECSPYWQSTPSPAAARYRPSGKNTYQPTSAEVLLAKSIGMAHGTATAAAAARMYGNNWNVNLATAATREPVSAGRAGGGGGGGGGGGGGAGNEIHLSDPRLSSPPPVAAMLTALPAEADRRMMSYSSSGTGTSFASAVNSVKAPPQTPPPLPPWPVPGVRAKDSGCSSGPATAAAAAAGAAASGGMDALLERLAAEAFPSSPHSLTPRSSASEPVPSATSAAGTFSGVGGLLLQGDPQASVTPAAAAAAAASTTVNADASSVTRGVGGGDHLHLPSRTNAPPSAMVDGVRQQLQQLQQQLKRVQEQVAAVLSGDCGSITIAAESAQSIKAMAATAAATATATAAATATATATMKQQQQQEQQEVKSNVRGGSGAAAAASKAPALASGAVVVPTVAAAGRMGSPLSTSTSRGGPFRLVNPARILGSILRSVRPPGGGGGRPSASPSPSRDVTGASMTFAIHDIPNDDAASESAAIAASGTAAADPAIASASHRADSEEPNRFSHHRLTSLTGTATAVGADVSIAAADADVTGERPSTLAQYSHISHPPWNILRKASTVVPAAAADATFVGQNGGITSVATPVAADDNAEVHDKQLKELQGQMLELQKLRCQLETQQLEQEEEMLQQQQRAHRTAGDDQPEVSWSVSSTGPVHQQQQRLQLQLLQQELVNVLQQVRHQEDALRHQHQHIEAQYYRQLQQKQHDERSREGERSCEGEQGENMLYTSGFESKVIYPAVADPGIVQSAASVAGEAEVLNVPMPDLTSWEPLPMPMPMPPPPPSPPPPLLRGPATAWSKWPRPPALPSPPIQPYAVPPGYAVVSNEIFKAAAELSRGTDAGDGGGSGAADSSIAATAASAPAMVLLPMEYLSGGAMQEGVSAISDVGGGGGGGGQLLPKLSTPLSGSAVAQLDGVRLVLPAMMVVDSRALPPERLSMGCTIQQEQQQQQYHHHHATYRVGGSANFERRGSRCNGVSKALLASHPLTLDYGADLLPDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLQ8</accession>
    <name>A0ABQ5RLQ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MGS-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_000124</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PurH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01421">
        <property type="entry name" value="IMPCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002049">
        <property type="entry name" value="PRK00881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="8">
        <location>
            <begin position="70"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI58421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="606" mass="64932" checksum="AAA0F24F057C17B0" modified="2025-10-08" version="1">MACLQRAQKSVVVRGEQQVVSAAVIQPRFVAMPLLAGRSVVAEASSLLLIGRAARQQLNATAAPPAPAPSAMPSQANTGTPRALISLSDKTNLDVLVKGLSELGIEIVSTGGTAMAIQDAGVPCVKVEDVTGFPEMLDGRVKTLHPAVHGGILAIRDKPEHMAAIAKHNIGTIDLVIVNLYPFRKTVTSDPAPAFEVSVENIDIGGPAMIRAAAKNMAHVAVVVDPADYPELLEKLRQGAGSADFAEFRKKLAWKAFQHCSSYDAQVSEWLWSQVGSGPAPQMTVPLQLAQTLRYGENPHQPAAFYTDSSLREFNAGGVATSVVHWGKEMSYNNYLDADAAYQCCCDYTGPTCVIVKHTNPCGIASRSDLKEAYRLAVRADPISAFGGIVAFNRSVDADLAREIREFRSPQDNETKMFYEIVVAPGYTPEGLEVLKGKSKNLRILEAKPRAPSGISLRQVAGGWLWQGADSLNPEAITFTCVSQAQPTAKQLEDLKFAWRAVKHVKSNAITIAKDGRLLGMGSGQPNRVNSVRIAIEKAAAEVQGSVLASDAFFPFSWGDSVEIACKAGVSAIVHPGGSLRDQDAIDCCNKYGVVLLTTGVRHFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMM1</accession>
    <name>A0ABQ5RMM1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">MLO-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_000581</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MLO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004326">
        <property type="entry name" value="Mlo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942">
        <property type="entry name" value="MLO-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942:SF52">
        <property type="entry name" value="MLO-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03094">
        <property type="entry name" value="Mlo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="382"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="590"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="637"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="649"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="706"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI58818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="888" mass="91215" checksum="36BA892898C5441D" modified="2025-10-08" version="1" fragment="single">MAEGGANLDGWEISVLFVLFMCISCAWQIFIAIITFLNNFYGPDAKKIWLTRLKEEVLDVGVISLALVFVQPYIANICVSSSQDEYPSPPPPGAPVLPSSAPPDLPSESPGRRLIEILSAAPIAARRALAGASADPCPPGKRHLFPANSISTAHYLLFFVALVHIVYSLFTFTLTLQRFSTWARWERHTRLAEMDGAEKPTTDESAPAALEEAPDPPSASRWRRCSPRRCMVGCWRSLTGGVDPARYKVLRGMFQLRARAYGATSFGSHNLHYSMICENAMQIDIENVVAHGWLQALIVAIFTLYTTKAYQIVWVGGLSIIAQVVVMVKLQSVMVTIQRIMTVESLCFGSKYRINTTSRAYQELSFAVPSSEDGSRRQIRFSHLPDADDPGGEDWPAVGGGQTTLRRRSDSSHWTQANHGARGGARKVRSLPSRSVALAVASSGNAGGAGGGGGGGMTRAFATSSTFGGFGGGRNAGGMPHKGRSADAPPQPLGRMSRGPRPLAEGWEDVAVAEAEAPSLSRLASGHSWWMSAEAPAEAESSDPWGFCRTAGIPYGPAASAGMSPWARALQPWADGGLVGWAGKEAAASSAMQGGASRKRLWGPGPVGGVPAARSDGGGRLPAGAPGPVAEAQDWGFGSGGSRGGRGSRGATRGRSNSASRSSGGGGAGAAAGKAAVSSGGGRGGRINIMARGGFDTVIEPASLGSGVGPGPGGAGAGSEADTERGGLGHFLAPGYTTDHGNPLYDAEQGTASLPELLASQQQSGNFTVASGGGNGGGSGGGWGVFGNAAANAAAALANPGTTGGDGGDHSKLSAAVSGRLGWFGGKVATGDGGGGGAGRTSAACSTAPTSGCYGELLEVASSSRFANPLFREGTEHSESLTRDAVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMV1</accession>
    <name>A0ABQ5RMV1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the citrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF8">
        <property type="entry name" value="CITRATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="134" mass="15017" checksum="0BA0AA3594CCD599" modified="2025-10-08" version="1" fragment="single">HFAAAYRRGAPKSRLWETVYEDSMDLIAKLPQLAAMIYRRTYKGGHFIQPSHTLDWAANLAHMMGYEDSSCHEMMRMYQTIHSDHEGGNVSAHTTHLVGSALSDPYLSYAAGMNGLAGPLHGLANQEVLRWLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMV3</accession>
    <name>A0ABQ5RMV3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="699" mass="74908" checksum="2871E3DE7BC00CBD" modified="2025-10-08" version="1">MKDSDGPDRSACKHRFSRPQDTQALPQATRSPNLLSRNMHIYVSTYSSGQMMPGTFPQPASEKLRTAITRSNAVTLGDVTHADLRGIVSRNLLSNLDLRPTSERISTGPDLSTLDRVAEDVASISIRAPQGPTPDASKAGGCFSVSLNPAITEDLSTAGCWTTIYMDRGCYNLTTARSSDNGICALTQLAGPDGPMAGWLLQLSPSLRGLSQETAGRMPNPRNQAGDLAGAVRAAAATESNTAVTYDEDVAVRVGLCGPATPTTAVCPRSSAPAEGAAEDAVCYGRLEWPQMSLSPTCLSMPPPRCSECSSPCSHDVDETVDLASLASDEPYHVATHCCRTGILSLACLKKASSASKGWTVRESLRQLVAAPLSDPIHHLPRGIREVVEPVPWRGAINSPRADNQPLVIAGDVCSDRGPRAKQPDPFLHPEHFKGLRTGVLAVSINPKSESVAGWEDFLENSSALKGSQAESAGRWDMDCSTTATQHSGVKSGIYTETPFRASTVELSGVGDAYGAVWAGEGADYVSSRSNGYGSDVDLDGWSVDKDPTNWVPLRHRYCILSVNVNDGKARMEVADGRMSRRVCEDADPVPDDSARDYRSELYLDAAIVESDPLKWTPLRVRYAVTPQNKSSVALLHNPGGRVRLLALAFERRIAELQSSYLPRHQAFVKPTRDHGCVSPGGTGSAGLCRVFSPVRLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPD0</accession>
    <name>A0ABQ5RPD0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000996</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039226">
        <property type="entry name" value="Ski3/TTC37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704">
        <property type="entry name" value="SUPERKILLER 3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704:SF7">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="835"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1562" mass="163903" checksum="CCBC8986330BF8D6" modified="2025-10-08" version="1" fragment="single">MSSSSVKRLLRNARECLGRREYKDALQHCKEALAEDKSSYEAYIYVGKAAFHLKEYDKAEAAYRRASNFEPPNPLAWQGLAELFTETGAWANAVECYSQLLLLAEAAAEGDPLRGRRTIFQRRFAEAAARGRMLAEAEAAWRSLTEAAGESLAAAAASAGGGGGDDGASVAAEEEFVECLCRLADVQLAVDAAEIEARVEQRLAAEGAATPNVTTEQMKSAIRGTVEGEWAEEALELDADHASATLRRIIALRPPSPPYVPYYDAFLKRLRKYIQASPPGSMDRHSRRAVLLAAAKATMEGRCGDRTGGCCSPHPFETALAMLEVEHEVVGEQDPSLDPASFPPSLEPPPLPLLVADCDRIATRLFHSFPWNPTAQLHAALSLRRRAAVYGFDFAAAPGTASHGVARLPAAGGRPLAGGGRRKQVTALLLSALTRGGRAVSGWLAACELLLEEGTAKAALDAAKEGLKYVAHRDLMGKERLAGAGLLLRLLAGQSLLRLGKLDEAELLLEGLAHGVSEGHVASGELAGIPPLNVSQHAKRSLAHVAAARGDLAGARQRYDELVGGHMMGRSAAPIEAWAHGELGMLLLRQGTTVEGARHHLQAALAALQPQLDTAAAAAASSSATVLQHLQHEAATYQLHLALALWVKGGGSLPYSDSTSVGGEGDATTAAAGPSLEPYRADCRELLLSAAAVAGPLQAAAFTWLGRWYDWVSEPGDEVKARRCYQRALALDPSQAAAGKALVAALCAAGRPETAVQLCEEMLARANAAAGTNVEAAPALPYLVYPGLLSDAAAAASGSGGWALRGLARLQRDGGSNEAAVANYQAAIRLEPADSELWEGLAASYQALGRHTAALKSYGRALELSPGRLFSRIQAAALYYQMGDMPAALSYYRMALTGNAGNAAARLGCGEVLLVAAALAARMGAAGAAAAALAEAEGLAVHTTVQYGNLQAGWKLLGDVRIQHAAVPTLESIKAVVTHGSMTPLDIANGGSTAAASVQDGLTAARDRLAQLRAARRAYAAAVHLDPRVAELWGDLGMSYHLQLELEGQHPDLVTPANHRIRLRNRAFALARGGLRLDPISDWLWSCSGTIAAAAATESPAMAAVAEYCYSRALQLNPRRAPVWAALGRMYATHGEGGLASRCFDAGRGHEPTSIAVWEAMGDAALRRGGVVDGSGATVPWVRPAWRDAADAYEHAQLLGGDVESRLGFVLGSISARDGAAAGTVLAAATKAAAMYPLLASAHHARGISLEARGDYAAAIRAFQTALVLLDVDDLRHPELDHSSSAPSVMPFDPRVTPTPRVAVQLDLARALSAAGRANEAVQLYETLRQEGVLRAEDFAAVLSYGVALHQAGRSSEAVTHLVAVLSAAGATPHLRLAAATAAVRIHIPARRWDEAYGIVLTATSDLPDEGYHSTGSSRAAAAAAAAAAAAAAAAAASPDPESAAKLWRILLAGVVSARASELEPQVLAGMRQWTERRRDLDTAQHECRVHELLSAKHRAAGADTSALREAALAVRACPYDRAAWALLSHTAVQDPSLRHAALAARVAPRARAPYMPAAADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ03</accession>
    <name>A0ABQ5RQ03_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001534</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF44">
        <property type="entry name" value="PROTEIN ZNTC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="397" mass="41523" checksum="2CB1132872C638F9" modified="2025-10-08" version="1">MYRLRFARALLQSLNAEEDENQLDNPAERSLDNTETIRIVSIFVIFFTGLLGGLPPLFLKVFRDPDSVLTRTVRSFAAGVIAALALVHIIPEAVKEMSGLGGIDYPLGGTCALGGIALMILLEHVAHIMHNGENNAVSEVGIVHLHKSSGASKQFLGAVSVDSSPCKVAARGGNATAAVAASAVANCDLKVQHGGSDLQNMDSSVTAGCTALHGHSHVCVSHGSASNWIAAVAMGSLRLKVIAYLFELGCIFHSFIIGLSLGVNQTDIKEVRSLLIALAFHQWLEGISLASVVTRGGFSALKGGLMILTYSLTCPLGIAVGMAIAETYDAESAKSRGIQGAFNGVSGGMLLYISLVQLVAEDMGRLVPGRDSAMNRMACFVALCGGAASMCLLAVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQB3</accession>
    <name>A0ABQ5RQB3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL23c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Large ribosomal subunit protein uL23m</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001139</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL23M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059">
        <property type="entry name" value="RIBOSOMAL PROTEIN L23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="264" mass="29577" checksum="2F886629CA243CF4" modified="2025-10-08" version="1">MAAPNRICHLLLNKGCLPHTQTGRGGTLEILSTSAGWRSQHTAGNLASLSGTASDASQAAKGASSSVKSFTKASSRLKQLDEEPDDLDEPPLPPLRFVSHEQRTVRQMPPRRIPICFPNITLQMLKLRDKQLAQLRETGWLREVAFRTAPEVTKLEIAAFLEAVYGMSVERVHTINYLGRARLDAPPGKNKRHFYRDDDWKKAYVVFRPPPGMGHLVLQQQQEDGDEGSGSVEDERPVIDQIREAAHSPRAEPKPLPWRRRPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR80</accession>
    <name>A0ABQ5RR80_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_002108</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="6">
        <location>
            <begin position="117"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="316" mass="35361" checksum="CB82D5D7F43BD333" modified="2025-10-08" version="1">MATGPPENPFTDLDSHIQKLLKCEPLPEADVKALCEKAKEILAEESNVQPVRCPVTVCGDIHGQFNDLLELFRIGGDSPNTNYLFMGDYVDRGYHSVETVSLLVALKVRFRDRITILRGNHESRQITQVYGFYDECLRKYGSAEVWKSFTDLFDYLPLTGLVEGKIFCLHGGLSPTLEALDHIRALDRIQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGADISAAYNHANGLDLISRAHQLVMEGYNWSHEQAVVTIFSAPNYCYRCGNLAAIMEVSDDMSRTFQKFEPAPRRGEPETAFRAGGTTPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRD9</accession>
    <name>A0ABQ5RRD9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein phosphatase inhibitor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="27863" checksum="72A61E2E974C6EA9" modified="2025-10-08" version="1">MRKADSARKRPAVGIRWDEQNLQDNEVIKATINKTKINEPKTPYHGPLGDQHMEDVDDGMQPLELDSHPQQHPAPSLGVSSAVDDDPCTGTTREAVANEDVAPDTPEAAQSGAASEAANGCSASVTAGSSGSSDHKHVHGHGHHAHFQPSLVLEDVAGEPENPSGFSSESERRISLTDGSDGELNGDESRRKFEQRRRAHYNMRAALRRARMLMSEDEDERNSDSEDGLGRMATCSTDAAPDADVTMSDGGKQAGGDTAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRW5</accession>
    <name>A0ABQ5RRW5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002057</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="172"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="273" mass="30349" checksum="2372FDCAF2C631B5" modified="2025-10-08" version="1">MDQLNGRSGVNTVAGATHAGEKGLEQPSSSGLEAYRLLYPVGSRKWNCRAAPNKRSELLGAYHDTQTILARQVFYDGSPWLAIPLPDGRVGWCKQYDEAHMVQHHQTRTGYGWYRVVYCSLKDQHIYGVGLETLTPELLRDLAAEPPAPPLHLGAPRSGGGTGATGCKRDRCASCFLPLRAGTMAYIASCGHSYHYDCLRQLGDANICTACGVWYAVGQAPPESIPAPYPTSIFQHVHQQLWFVPPFYRYILIYVAMLLMMPFFYVASWLHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSV4</accession>
    <name>A0ABQ5RSV4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_002880</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family. Importin beta-1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="10">
        <location>
            <begin position="25"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="407"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI60678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="904" mass="99254" checksum="EFB5C6EF3EEE0619" modified="2025-10-08" version="1">MATVIDLSAALASTFSHDANIRQQAEQHLDHLKESNFPGYLASITNELGNEERADDIRQAAALQLKNSVDAKDAARRQDLMLKWQGTDAALKQHIRDVLLRCLHSPKGDVRKTTALVIAKIANIDIQSNAWPTLIPTLLNNMAAQPPAPVGTRQATLTTFGYICEEIDESLLSSENVNMILTAVVAGMGESEQDEVRLAAITALTNAIHLARNNFEVETERNYLMTVVCKCTLASSQPMRVAAFQCLQQIADNYYSKLQSYMTELYGLSTKSIKDDEDEVATQAIEFWSTVAEYELDMMDDGKDDQCKNFTASAAEYLLPLMLECLTKQDEEALEDEGTWNRAMAAGFFLKLLARICKDRVVPQVLPFVTGNISSPDWHFREAATFAFGSIMEGPSPASLDQFVRAGLPFLTNALKDQHRVVRETTAWALGQVFEHLHGNEAENQHPIVPKESIPALLSALVASLKDEPRVVYYVCDALRYLALGFQSPDGETTPLSPYLKDLVQNLYEAAERFRRDQCDAAVGHWDPREGAKAQIAAFEAINDLVRSSARDTLEFVGTLLQVVLSAINANLETQITTHQAAEKMATLQGQFCGMLQVCMERLCRSDDARAALIPFRDKIMETLLQVMARTQSGAGVHEEAMLAVGTFTVAVGSDFEKYLPGFMPFVRAGLQDHMQWQVCLSTVGVLGDLCRAVGTAIWPYCDELVSIVLANLGSPNVHRNIKPELLTVLGDMAFAIEGNFAKYLDAVLTILRQAMAMSIQMVSSGDYDTYDYNNMLRRGIMDAFSGIVQGLADDLTKGTNLGPASRDRLMAELPAVFQFVSSIGADKTGGDTYDEDVARAAISLLGDMCSVLPGAGVALANSSSKEWTQVIQFILSCGDDSLSSNNVNWAIEVLRKATNGAYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT55</accession>
    <name>A0ABQ5RT55_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002570</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045123">
        <property type="entry name" value="METTL14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107:SF0">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51592">
        <property type="entry name" value="SAM_MTA70L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="125"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="572"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="587"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="600" mass="62169" checksum="259CF4C130699EAD" modified="2025-10-08" version="1">MQDNSGLQAGVRGGRGRGGRGGNRPGAVMFARGGGNQRQNGSGQDAPSGMFSEGAGMVPRPPMPGGTMMSMDINPEMLFNMTMGGPMGGPLGGPMAAMSAMGMGLTDFGAGGAAPGFLQAGRGMGPPGSSGGMMGGGPMQGPGGHGAGQGAGGPGSGMHGLSGSGPGGAGPMGMGPMGPMGMGPMGPMGMMAGGQGPMAGPMGPMMSMGGMPGGMAGVGAAGAGMMGSGTSGMGPMGAGGAGPMGQLVRPGAGGGGSVGPGGAGATASADIANDYSQNFVDTALRPQNYLRDSQLTDRYEEYPKLKELITRKDKQVAAYATPPMFLRSDLRSLRLSPETFGTKFDVILVDPPWEEYVRRAPGLVADPEVWSWQDIQALEIEAIADTPCFLFLWCGAEEGLEAGRVCMQKWGFRRCEDICWIKTNKEQGKRPYLKPTNQHPESMLVHTKEHCLMGIKGTVRRATDGHIIHTNVDTDVIVSEEPELGSTRKPEELYLVIERFCNGRRRLELFGEDHNIREGWLTVGRSLTSSNFSAKAYSDHFRNRDGSVWVQNVVGPKPPPGSVILVPSTEEIESLRPKSPPGHGAGHHANSSHQGSSMGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTM4</accession>
    <name>A0ABQ5RTM4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002694</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48466:SF2">
        <property type="entry name" value="OS10G0509000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48466">
        <property type="entry name" value="OS10G0509000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein MutS core" evidence="6">
        <location>
            <begin position="93"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="7">
        <location>
            <begin position="545"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="458"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="814"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="902"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1013"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1084"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1190"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="740"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="498"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="911"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1035"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1084"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1094"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1131"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1217" mass="127112" checksum="A6FCE5209E13C2B6" modified="2025-10-08" version="1" fragment="single">MQYHLHNTRWPTASRPRRPAGNRLKIFFNTALRFRHQHCFCPASNRELSTLQRSAVARATLKPYDALDRQTGWDAAAGYDIVGPAFQATLDMLEWPKLCEQVARFASTHAGKRACRALHVPEDPRETLRLLEQTRAMTILEYDWATSLDYGGIQTSEAESGLSRAAKGGMLSAEQLRAIVTLVNGSEKLRKQVVAAARDNDSLRPDSPVRVLLAAVGQLRQQPLLVRSVLMAIDEDANLQDSASEQLRGLRARVKAIQSRLASLLKGYGEVSDRGGRMCVAVPAGTKISNGVLLGSSPGGGLQFIEPPAAMGLNNELGAARAEAQAAEEAVLWQLTGLVLGSLDELESTFRVVAWLDVLSARARYGIWIQGVLPEIVPWDTVFHARGGAAMRRRARVEDGTAGEESGYSDLNLLRDPEERYAVRLRGLRHPLLYGEYLTQKESLERFVRMNPMSAAAGGASPSLLPSGGPVGGDGGGSGAVPRRRLLSTRKEVMLRKSGSGSDSQYGSGSDSEGEGDGADTDSPHAALYALKPPRAIDWVTRPDTCVVVITGPNTGGKTAAMKALGLAACMAKAGLPLPAEAPARLPAFSAVLADIGDEQSLTANLSTFSGHLKRIQALRAEADGKALLLLDELGTGTDPLEGAALGVALLKKLVNGGVGSGALTVATTHHSLMTGLKFDDPRFENASVEFDDAALAPTYKLLWGIPGRSNALNIAARLGLDESVVSGARGRLDSGVGTVNSAIEQLEGLRGQLEAEERATWGADREVKTLTRKLKAMHAAVEALHERLTRARAEALLQVYTLARDRIRQVKESRRRLGRAAPPPKPLLAQSADPAQLGGTAAAAASESRAGYAGAASAGRDAAQPASVPTEEEIWEAWEAATRSRTTGASPVVGISAQTIGGPTAPVPAPSAQAPAAAPAVRQGLPAAPSSSAAGSSSAAETPTPDPLRSLMDLSGDLSEGTLGLEALVDDLVAQERQAVMGQALGQQREEALYDATLAALDGILDVAAKSPATGQQQLQQEPPQRRSPKSGRARAAGRAANAAAEGGPANEGGEDDADLAALMAILEEGEAALNARMRQLAANNGDNGSEPSGSANGSTNDSGGGNGEDDAFEPSRQTPQGRGAEGTMRSSSSSSSSSSSSSSSRNSNSNGAIGGGAGVQAAGEDDELDNQLGDLDALVEEYTLALHAGGRQGQGQGQVTALRSSTADARVTDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU28</accession>
    <name>A0ABQ5RU28_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002822</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052994">
        <property type="entry name" value="Tiny_macrocysts_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057352">
        <property type="entry name" value="TPR_TmcB/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600:SF2">
        <property type="entry name" value="GAMETE ENRICHED GENE 10 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31600">
        <property type="entry name" value="TINY MACROCYSTS PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25474">
        <property type="entry name" value="TPR_TmcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0157">Chromophore</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1720"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2150"/>
            <end position="2169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="2362"/>
            <end position="2388"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="629"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1402"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1585"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1980"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1461"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1587"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1621"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1982"/>
            <end position="1993"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2498" mass="269988" checksum="EC59F328AA976BE3" modified="2025-10-08" version="1">MAGSVVRSASGSYAGSVASSRSSASQRARAEHEHREGAGDDLLDRRSSLESGIFGVLYTLRKENNETRVRIRWVLLKILLDAWQLFATVISPEGQGWTINAHGWAWTVVSVVNFNWLADLSYGWYLVLLYVMVGLLGLHVVLCVWVAWCFKEQKFGVVWPIKLLRLYSSVFFQAFDVAAMNLLELGITCRFTGYPSDTRMRFDLFPEYSCSSTPHVMHAVVSGLGLGFFVAVALLSFIAEVEVNLLSRRPFALGHSGPAVIAFAFKVLITLVRVFIGWRRVQAGLYLGFSLAFAWQFLRKGPHLVNWVNYLKAGVSTLIVWCSAVLMLLVFHPGIKEHQMKGWADIMTLLMIVGLAPAFAVGALLSWALIRRLTNVTLAELKNAKPGAPVKDICTAVDSPLDVAIVSRCCRVWKDKYTVDPVAADKARHVIQAGIAMFPTSAYMVLLQANFMIDVLGVSQSGSRRIEDARKLNPGLMSRFIMFVRQQEASQQAAGSYASGGASMDLLAYVEYQRKQRMVVRLHREALQAMCNFWKMLESSRVSFTLLSKALAKIETSVSQAQTAYRVVLENYGSNPKLVRLYGKFLLTIKNDPWRASEYFTEADRLQEMQNGDAGGGPLLPDGTPLGRMDELATAVLVISANGDVQMANRQAHLLFGYKRGTLEGKPLATLLAPHYARWLADHLAYLADCTDSVILAATSAHDLSQQLPQQQQQQQQQLDGGSNNFCETVVGMHSDRLAFPMKLALRKASGVAEDSTFIAMMEVMPAMKGVASLWVSNNGTIVACDPQFVMSFGWKPQEVNGVMLTTLMAFQNLDLASSAPTAVDLELQKQQRDLSTPVEATATAQSLFVSPTEAVNRLLRLAMSNSEATQEGLQCLTAHKYSSTPLQCMACVQGSGGALDSSVYTVRIAVRDAASSGQDMLVVNRKGAIVHASADVLIHLTGIAEGRGGVSQQPGGSSFLRPKRDGPKPIQPAVAGALVPTALMMGVEQLEGFTLCDLMPSPWKEMHYKYLRGTTALTPASHNLWSCRKEGIMGPTLEMRNTNGKPLFMRIAVSTTDTSVEINHVIKMSLSSLEAALGERRLRLQVSGEGVISSVQEGPAAGLLGLNCSQLSGRPFWDFIDWSRYAESGSLPAMGPLMFAALVKREMLNRGTSWRVQVSPPVRMMSEAAGNMSDLMALARSSMKKTALLQVHIEIPSEMETAAGADPAIFVDLWCQNSVSGVLEVDGVGRIRAIVEEHIRPSGLLFGVATQSLVGSQLGEFVAMPAGRTKPADLLFLNGAKKSSLKTTTAESSVKVGPVHVLQAAHADGLPNMLDVQVVSKTGSNSNMYVVLRPHTVPMFPTAGVPSSPPPPPRPPGAGVAFGAVIPTGGTTGAGSYLQHRPSTASDPKLPATKALAYPAAADNNCRKSPKSSSTGGGVVAVSTPPEHPLKRRCETTLPADDNNGSEPLVPPSSLLDPLALPPPPPPPDGLARLGGGLPVPVVVPTAVVKAVAPPPITSREKLACMVKSVQGSRQATASDRTALGGGEVAPIAAPLPAAADVESVNGEADGHDGAPPNVALNDKERISTWVASKGAYYQNSVLADGRGDKGEEDGGKRLDSIDGLPENLGPRELKAIFRGDGGGGGGGGGSDDDDLDDAKTTTEEPLGSGTHNWAWRRQEVNGASCPAEDDAGSEGGQSALSGQSGDGGADYKRGKRFRKLMKLMDSGQTQQVQQRYRLHALVTVALLAAVHIVCFAMTMHSIRTKRDSMLQLGRSGQAQRYMHQILTDVRSLDMISKNKSHPNLYNASKADAEFFIQRIAKSADEVQIRVKNILDSHHTSTTNVRDLLFFTTRTVWDGKDDNGTDKFANITIWDFCTRFYAMAKEVQQNGMDWVNNHVDIVGNTTAGQFLIRSGPDLWKASRKILDGLLYVAVDDAQWVDNLQIVFLCVEGAAITSVAACYLAYLLKAVSEQRYKLYSTFMHIPLGLTRALASQNTTLTVDEEDDEDDSNDGDEKGSYVEDNEDIGGDEAALKQKRRAMLAVSDNNNNNSCSPPLEGYNSALGHGRDALPTSPKSAAADGAFLGTHYRRNSRSFKAHHSHGHHSSSSPIVAMGAKSKTSSGSVLRHFAGRLRGMVSRMGARIVPLQNGSEGALEARRQLKYDSHETRMLLMPFIIWSALVITIYVVAVSKMSGIVEVLAVHSVVNFIAARTYRAVFYCQELAAVVDKSKLPQARAAVAAVWKVVQDAWYTLQLGSDAYKAIGPNTERFPLVKRGLASSSGVLSYIFYGDGQCHRVPESRPCPGPEYRFYLSIRAGLDSMMHQFMMHVDAMASSKATDAPGLGDAHLDFIYNVGTKDLMDGTVEIQEAHSHIVMTLFDDLLILHVILLILFWAIFASFLIFLLNPLLKRISRERRRVAELMSQLPLELDVERLVARALGAVGNGGTPVAGAGGSIGSAAAGSVKANQQGGGETGTNSLAFDGNADAVTKWKSIIRAASSQLFKVQTNGSMHSSRRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUC2</accession>
    <name>A0ABQ5RUC2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158791">
        <property type="entry name" value="MgtE N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="126" mass="12445" checksum="9CD77D80B094CEC5" modified="2025-10-08" version="1" fragment="single">GALVQTMTPKATANLVTAMGTTLTAALVSAMRSQAVAELVLALGSATTADLVAAMTAASTAELVHIFGGAMVADMVVTLGTKRTAALVVAMDVDLTAGLLRGIGMSIVADIAGQMGAERVAEVAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUP2</accession>
    <name>A0ABQ5RUP2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glyoxysomal processing protease, glyoxysomal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039245">
        <property type="entry name" value="TYSND1/DEG15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21004:SF0">
        <property type="entry name" value="PEROXISOMAL LEADER PEPTIDE-PROCESSING PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21004">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="710"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="819"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="819"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1066" mass="107888" checksum="B3D89AD90CABB203" modified="2025-10-08" version="1">MRAEPLLILVSGETRNAEQHTGQLAFTTTAKGLQFTSCSGILLSAANLHDGLVLLPAAVLHRWLKPGITPQDSIEPPSLSADCAICAVGPATVLSGGAAMCIPIATVRVPAVSRSAAQLLQSLNRGTSSSDWAFGWHIGGSHCTPVEQGSFVLCWTQPVSGCSVDTGVAAGFTSAPDPPKPHIYATFAANEAANPASSNGSGATATWPGPHPRLDKWGTQYGNGDEGAAIARGTLTTPQCVALMASLYSSLSAARDCGIPGPISGNGGSATVGVEAVSLRQQVHATDCELAAPAAGSPRPNCASAETRPGCLVSIVGSPFGCLAPFHFSNAVFNGVIACALPEPHDHTAYIPAGGAAASGSANHVKTDSDVRCSCGPALYALDAHVFPGMEGALVTVLQAPGAAAVELGGSNCQGRTPPRGCPGHVGGSRSPAFPLQPLALLCIPVSRRADGVQVPLAAGWSHVAAALRDVLAQLLLRSQGASADGAAAVAHLISSTSTTREKQQLQLQPVAASSLMDAATAAACCVSGCCHARATCNGGMHSGPFEARGRASHSIGTGLWSVGIAGRHVARPHDSRAAAAATVGPLAAAALQPRSLSGILPAAPVPSACLLSRAMTAADSAQPGAYPHSCSGVPAAALLANATFATAMIAECSSAAVSPAALAAGAAAEGLPSWVLQAVVLLRYNGSWATGVIIESSTGRLVTTAHLFQRPSDSAGTPPHNRPTRGHGGGGDDTWARLQCWVRVPSIIGSGGSGAGGVNAACVTCGGARSSRGPVYRWVRARVLYMWSNHLDLAVLQLEPSYTSRWTSVPALAEVRHTARARDDATRDPPVFRPTPGSKVATLVNTDNAGAEVEPYSVYSPGTPVWAVGHSLVGPSAEWPALVSYGCVARVLRVHSGRPSMVIASTTTHAGGSGGALLDCHGRLVGLVTSNARHAGGITLPNMAFCIAAEELAPVIRWAAATASSSMMGTAQRGRGLSELAKLDVSDEDAARMWRLLMPSLEPPAPTDIAAAAAAAAAGKELIYGVDGTYRIQTTGTTGATGAGAAGDEGEVAAALLPRGGRSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUR7</accession>
    <name>A0ABQ5RUR7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003292</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF29">
        <property type="entry name" value="PUMMELIG, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="159"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="504"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="539" mass="58802" checksum="40DA1ED683E84EE7" modified="2025-10-08" version="1">MRKLGAQVDRGQAPTRLRVAVGNWLASAVVLLRGPRSTIYHRPRFSFQSLHFQAGASTGPGTGAGAGALAYCAQNSGQNSEHLTMDASSSVPPPTPVPWWTKYTRWRQTSLQEGLAAEHRVLRLFPGLGGPMQASDVKVGPGRDAYLHTLSGGPVSVDPPVVMMPGYGAGVGFYYRNFPSLAQRLRLFAVDWLGTGLSGRPPYRARTREQAEDFFLGSLAEWRRAAGLEGSKMILVGHSLGGYLAANYALRYPEHVQHLVLVCPAGVPKAPEDWERRWLGDKWSLRGQMFKVFMWGWEKGFTPGGLIRALGPWGQNLVYKYVANRFSHHGEGLSQREIDAFKEYFYSIAALPGSGEYALRHLLAPGAWAHAPLEERLHELRVPVTFIYGKHDWMRPEYAVRLCERLRAERSPSAPNDLSVEIIEDAGHFVFLDQPELFDKALTNVVRPYLVSDRERRRAAAYVAAGTDVHVAAEAMADARASGQAAGTATATALQQSPTATSWGFAASGVTGHMPVAPSGDPVDGGNPPTSEPGKGRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV08</accession>
    <name>A0ABQ5RV08_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003858</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45000">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45000:SF5">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="619"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="557"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="686" mass="77050" checksum="78DC1B5A4B878A2D" modified="2025-10-08" version="1">MVRLLLVGLTGWPSEFMQTLITPFMASAYKPWNRQIIIVGFRQASSSSSASTRHGELLKEFAHRLPNRLHPDDLHYLLKHLEGFAPPSMGVEAAKSCRGTTHQGTTDGAVAGAGAGNAANLTVADTEGQSNSSSDRAPLRSGWKLNCATTSTNVTNNGFDSAAASAGRSSHATGRSHSTTSDASGSSYRAFNIGTSRSSHRHGHSKQGQHNVYDDIYDDEYDHLHNMVFQRLPRQSHTHIRVELHGSPSAVSGTPEQGGGGEQDSYGRGRSGHTSSTRRSRSCNWQFSEPHLHHHTSGPGPDMGPNQQRMPRRWWRSWPPHGDRIWEEGEEEEEEEGGEGARGPRRHNSRRRRYETVRRPLGDDDTDKEEGEWASGVSTTQHRGRTHRHRCNDSDNSDTRHNSYQQTQRHQQQKHREEPRRRQCSETVSQQQQQQSRQQQHHHHHHAERRQGTRGVAWFSKRWPKHYVNYKEYVEFMSQDWNREESPQAGGGRIRSKFYQSAFEDDRDWFSQFWNDGTTGGDGSASKQSEPSYSRSRSAEQFTSGWWQRFQAAASDSGASSWSSSTSSSRGATGFSSDHSYNHHHHNHQRQQQHSFGGQHSGQYGGRQPDPQHYPELHTHMLVLGLDVGRSLDAAVVKAAFRARAKETHPDLQHGADAAVRTAAEERFKAVQQAYQALSRAVGAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV55</accession>
    <name>A0ABQ5RV55_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003141</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF24">
        <property type="entry name" value="NAD-SPECIFIC GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="8">
        <location>
            <begin position="213"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="446" mass="48883" checksum="ABA1770A040AA467" modified="2025-10-08" version="1">MVRSLLICAQQMGSAIGRLLNGQGLAACLPQLRHASSHAENTNTFLREALNRLDYPEKLQNLLLTPRREMSVELVVQMDDGQIEVFNAYRVQHNNARGPYKGGLRYHPQVDLDDVRSLASLMTWKTAVMDIPYGGAKGGVTVDPRKLSERELEKMTRKLVVAIKEIIGTYEDIPAPDMNTDAKVMAWFFDEYSKYKGFSPGVVTGKPVYLHGSLGREAATGRGTTFAIRELLKALHMGKLGDQNYVIQGFGNVGAWAAQLLWEAGGKVVAVSDVAGAIANENGLDIGALRRHMSSGKTLASFPDGAPLKKEDILAVPCDVLIPAAIGGVIGPEMAAKLQCKVVVEAANGPTTPEGDLVLRDRGITVLPDIYTNGGGVTVSFFEWVQNLQNFKWEEEDVNRKLDRKMTDAFNALWTVHREMAVPLRTAAFVVALQRVTRAEVHRGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVS7</accession>
    <name>A0ABQ5RVS7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle 19 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004142</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="124"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="134"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="18607" checksum="434A087B0D78E96F" modified="2025-10-08" version="1" fragment="single">LFVTLPSCDCTLINVQRKLVTMNPDRRVIVYPQYIDALKTVAEGRRIPKSLACEDPNVGEMHDSCNLLKLHSTIEGKHYPRDWLPRCRIRVQLKGDDGKPVDPEIPDRRTLLVKLAELVVKHPGRVGGKPRQQSAASKLAEAASAAVGAGPSSGAGSGSKPAPSKKGKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVX9</accession>
    <name>A0ABQ5RVX9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004003</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF6">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UPL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="7">
        <location>
            <begin position="950"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="1279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1311" mass="140386" checksum="B3AE0362CDB2574F" modified="2025-10-08" version="1">MFFPPSRNDIRGKAHGGRGGRSKVELSAREELAKAREERELRKRQKLELKAAVAIQAAWRSWSSRLTTKNAARINWTEQYGYEGEKLQARDCLSSKTSGALGRLMFFYRPGTDWRLLVSVARIVASASKTDEFKTYTASYNGEDTSASMLYQLKALALQCFLTLSHHRYDFAPQLAMSRPSNPLDPLVGVAAGALLECVVLLVSPDPFAPVLGKPRATSLAAAVLGHLAANGMCGHLFLILMATLSPPLRPQLQLGLGNAAPAPVRQIVCAKPYVETLTTQLTVRLLALKSFNPTRVDDSLHVLLFVPDLWQRCTSLQPVSARLCRVALSWLSAAGPQRLGNVLQPPQCSALPWGTGTAVAILLANILAVGPRLLEGDKAAAVPGTQRALALNVVRVLHAVVSLLPLQPFFPDASVGGDETEDEVDEAACGSLGVARLMLPAGAAPQLPVGLVEAVQGLTGPAGRQLIKQLVTVLLPHAGPLPCEAESTGTVLWAPGLPQLPGGHTSTSTMSNSPPAVFPQAVICSGATYGVAATPQRVPSTGLSPQHSGSGIPLPAPILPSSGALLEAAEAAHALCEFLWELGQLPGQQQRVMLSAAVSCDVVERMWWSFLRHARHADLCGTAWLPTSPPPGSSPGSTRATCSASSSYNCAGPVSMELELPSLMTSAGGGTGANGIGAVRQAAGLMTGFAQSRSGSGATLLDGGGVGIIDAGGGPCSGGSGGGRGAASLWRPRLAEDAGWLLPLAVTCSAFSAQIATSTLEEFYGEAQRPLPLQQLYDAAAPEAGFLAMLRDALWQVLWSDGETDRTPAGEALYAVLARTGGLLYGILYERNCRRPFCPTDAFHATALAPDWFFTEANSSRSHAGREDGTTRVWQVLRHAPYLVPFSDRARLFQQMVGKERQEYREQEESRALEMSALEGLLGNRFVHIRRDRLLFDGFDNLNSLGDRLRGRVRIAFIDAHGAPEAGVDGGGLFKDFMEELMRAGLSAEYGMFASNSAHQVYPNPAAMRVVDDAPRLMAFLGRMLGKAMYENILLELPLAGFFLKKFRGTRCDLNDLPTLDPELYRSLLRLREHFTAASAGPATPEGSQHGGVAAADAMDLGLTFVVADDAAAALGDPHAEVELKPGGRCIPVTAENVNEYIHRVAHYKLNISPQPAVSAFLSGFYEMIPRSWVSMFSGEELQTLISGAEQRLDLEDMQENVVYAGGYHEAHPVVLLFWEALGSFNPEEQGKLLKFVTSCSRAPLLGFKYLEPKLCIQMSGGMLDPTATQRLPTASTCINLLKLPPYRTLTAMREKLLYAINSGAGFDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW62</accession>
    <name>A0ABQ5RW62_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome b561 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_004000</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08761">
        <property type="entry name" value="Cyt_b561_CYB561D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045150">
        <property type="entry name" value="CYB561D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422:SF45">
        <property type="entry name" value="CYTOCHROME B561 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422">
        <property type="entry name" value="OS05G0565100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="12">
        <location>
            <begin position="12"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI61584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="235" mass="25924" checksum="F8BDB2B8E45CE77F" modified="2025-10-08" version="1">MYATLTTTPSVVTKPTQHRIIGRLTLALHCVVACVALSIAFILFDNTLFAWHPAFMSIGYVVFMTEGLIAAIHFRQIDGHERVKAIWSHALLQLRAVICIVIGFGVIYRNKILLGKPHFVSLHAKFGLATLILSILMPLWGLVSFRRMGIIQRFPEVWQPRIKAAHRQLGFITWLLALVTIELALPHPAVKKGWLTLAWQGGVGVIAVLLSVLSAKAVGGPKHFSGPEDALCKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW64</accession>
    <name>A0ABQ5RW64_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_004368</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03884">
        <property type="entry name" value="M20_bAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01879">
        <property type="entry name" value="hydantase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494:SF19">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047362117" description="Peptidase M20 dimerisation domain-containing protein" evidence="9">
        <location>
            <begin position="23"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="10">
        <location>
            <begin position="277"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="512"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI61860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="532" mass="56561" checksum="26D1EC483869F6CA" modified="2025-10-08" version="1">MADWRLLIIIAACWAAIRLAVGETHEPDPFLRRTLHVEPNKHLQHLATISDTEGSLARTFFSPAHRRAAEQIKSWMQDTGMETWIDAVGNVHGRIKASRDGTQAVLLGSHYDTVLDGGAYDGALGIIVGLAAVKGMLLTELVQRGWLDRLPPQPANDMDGDIVIPPTLASSLLTHGSVHLVAFTDEEGVRFQSTFLGSRAVAGTLLKYGMLSSRDRSGNSLEQVLQDEGGVTDPRVAVASLALDPTTISEYVEVHIEQGPVLESRGLPLGVVAGIAGQTRLWVQVNGTQGHAGTVPMRNRRDALVAAAELITAVEALCTLPEGEGGVPEEDNLVCTVGEVKVWPGASNVISGHTGFSVDIRSKTDSVRNDVVRQVLQTVDGTCHRRGVACSVDRKHDAEAVLSDPDVLRNMGAAASLAKPLLLAAFTQEEVQRSSGMLEGLQSVPVLVSGAGHDAMAIAERIPKMGMMFVRCRGGVSHSPLEYVEPEDVTASTAALAVYLWGRLAGSLDAESEAAASEAPVDSGSSKARDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWC7</accession>
    <name>A0ABQ5RWC7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="396" mass="43431" checksum="E51CC9C0E840ED3E" modified="2025-10-08" version="1" fragment="single">KAGSEHRALLYLMQKVNRYFALDDMLPEVSPHIAAYHYLLLAPPKDGPVDGDAATSFRSSTLFLLSGMVLRRLKVMAKDPMTLGLLDSTVLQRRLPMRKLGIKFAGGLEGASGPASGSSGGGSQFARKASFASRLGPEASGGSESESGRHDSKRWASNRRPRRNGPPELIPVAIYSHPLYLDTAASIIRNFNRTGLIVTPYLYSTVVGAAAVCTIQGWWRSHLIRGMSNFGALLKMSRAARTIQRAWRTFMLRTRLHMLSTVRRLTAMVGSLLLGANLALTVGHCMALRERAGRPRPAPLFPEQRLRFAFDRGGGLMLVEPERMMPKPLRDGNWIPGLPPAPAPRSLPEWCGVPLPLCGERAAVSVHGCREVLGLESILQLLTRDTVWRCPPPRCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWJ1</accession>
    <name>A0ABQ5RWJ1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="346" mass="37230" checksum="FB482BBDFF1D5689" modified="2026-01-28" version="1">MSAEVAPSGVSMTSNGGLRASRGATLGLQMPKEPLPQHIPKIVRVEKKHTLKGHADAVTSLCFSPDSFLLASGSDDNTVRMWDVQSGNLRTIFTGHNAKVHALNFIGSGTILFSVSKDRTIIEWDLLRGILRMTLEGHAAPVYGVCVSKDSQKIITCSHDETIRVWEIMKGNLQKTVKAHTSTVYSVVLSPDGKLIATASADKTVKVWELATGELKDTLIGHTSHVVGVAFTPDGKKLLSAGWDETIKCWDVETGEVLHTFTGHQGKVHCVCTAPDGDTFFSGGEDKTIKLWRISSGSCFHTIHPDPLGKSSHSDEVLAVAIAPDQSILASASADNSIRTWKVIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXJ8</accession>
    <name>A0ABQ5RXJ8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004954</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.810">
        <property type="entry name" value="Photosystem II Pbs27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01481">
        <property type="entry name" value="PSII_Psb27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025585">
        <property type="entry name" value="PSII_Psb27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038450">
        <property type="entry name" value="PSII_Psb27_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34041:SF3">
        <property type="entry name" value="PHOTOSYSTEM II D1 PRECURSOR PROCESSING PROTEIN PSB27-H2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34041">
        <property type="entry name" value="PHOTOSYSTEM II REPAIR PROTEIN PSB27-H1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13326">
        <property type="entry name" value="PSII_Pbs27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="227" mass="25450" checksum="F1715221C67A7DBE" modified="2025-10-08" version="1" fragment="single">RFKMVTIKTQYLVSEHIASRRLVPFARFASAIHLRTQQQSCRASHDYPEAYNTYTVRSLNQKLDLLVDQPTRGAPHANHIIRRSTVFALVAGLLASQSHPALADEDAKLPKEYRQLVKRLSEGLSDSIEAEASGASEAEVRRKADPAKDTVREFVRKWRDNPRVSTDITHAELKEALSELGEFYMAYGQRTKLTPSVRESVLKHLSAAKEALPTEPKKNGSGLLGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXL9</accession>
    <name>A0ABQ5RXL9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anaphase-promoting complex subunit 1 middle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_004311</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024990">
        <property type="entry name" value="Apc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046794">
        <property type="entry name" value="Apc1_MidN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12827:SF3">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12827">
        <property type="entry name" value="MEIOTIC CHECKPOINT REGULATOR TSG24 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20518">
        <property type="entry name" value="Apc1_MidN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 1 middle" evidence="6">
        <location>
            <begin position="1569"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="430"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="575"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="665"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="878"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="914"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1062"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1241"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1317"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1677"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1778"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="488"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="514"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="575"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="888"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="916"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="934"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="957"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1084"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1245"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1680"/>
            <end position="1694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1697"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1713"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="2211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI61806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2211" mass="226825" checksum="05FD1F8AA13A6805" modified="2025-10-08" version="1" fragment="single">MEMNTRSAILLPEEPFGVQLLRREYERQEKMAQSVQGTRRRSSGDGHMPGDQKHDVPTSVDIRNSIKLYVREGAPVIGGDACIEEVAVLGHRVAWTSGSAVRKLFSLGSEVLQATWAVFPGHSSDPVLCLLCANCCLITCTMGGELQESAVPPDLDAMWPAGDCGVLLGGPRAVTLYLLSHPMDEPQPVIADVSLRAGRPGSYAGWSGEQVVWSSYELPYLATYSEAAGRLAVWTVGGAPLAPELLGNLPDIPAATPLGAAPVSVAATMAGPAGGVGCAPPTGMSAATAATPASMMSMFSFKPHVLAGAGVPSAAGQAAASSHQPHRGSAPMAPPLAQQATLVTPPVTGSGGGGGSLFRVGQQHSRVRPPPIPYGPSAASSDMSMAMSPAVTATATPLMTLGTVGQQRPPLPRSHSQQHYHLRHAFLQQHPSPLYPPSAAPSDMSIGCTPTSMHGDTPQTRQLQPQQPPLHPQQADQQSPPDYPPSAAPSDMSISVSMSIGSGRTGRRTGGPGPIATTPAAPGGNAGAAAPSLSGLPGSFPGRLYSPYGGYGGGGSGAGPSPMLMSPWMPTASPGPGFASSSAPGTGLKRNPATRTPEGFLSPHLFNPMPRATAAATAASPGCYGYAASAISPSPPLSHYAHHHGSRTGHSQWRPVGTPFSQFLGDTAGDGSGSGAGATTAPASTPGLLGATAGCRVGSRTHLSMAPRLGYGDSAEECTVMVLLHEETLQPGCRPTEAVMATDLHGTPLLCVLCRHQSRVLAFSISSPSGAAASVPMSLPDHKPTSGGIAGGSAIGSAGAADAATGRVRVRVSLTGSLEAVAVAAVAATGMPITSPSSPFRVHRDLLALRPSGQLLLHRGLSPLFVVSFGDLGGLERAGGGGAAASVPAQQPQQRNRPLSAEAGAFTAASGVRNAFGSNVDPSTPASATGADMVLDDGSDMDQDSDDERVGGGSTEAAPASHHGASSASVARAGGSSATRADEAMHEATDEADMMLSSPPTGGTAAAGPAAVLASWKCPPGSGAAVRVQRGGPAATAAVPAAVPKGLRRGVSNTIVLVLSVPPGQGQVSSSGEPVGSKHRSVKATPTQAPQAPQPTERELRLLLPFTTTSAAANLALEVIRTAAPAELYLTFLRRFYTYRAAATRHAAGEWAAVDAVLLEWASDPPAFADAGRAAGIRVQPSQHGRRGTSAAAIRSPGSAVYGSGPGSGYGAAMPATPFTTPLHTRRFAAMSLASPVVPLRHYQHQQQQQQQQGRQVRHQHLSSQRALQQEPVEGWAEAADGRRGVVEGVPAGAAGPGSGSAWERLLASESQARLMSSRRKPTWMTASDPGIPSTTTLRAGPTSRDLSGIHTLLGGGSGSVPAANTAADEDASTAFPVPPLGPAAQPLLAVLYGLHGLYEELKLDMLSWPLLQPLGSSLAAVAAALGLHSYWEHYARDLGPATLVAAGVPEPPLQGPQGLSIRLPADVYRILGRMVSGTAPEPSELPSLLDAGLTGTAVGTAALGAAGAGRTMNSGSAAATGGGPAVAMAPPPPPFHPALPLTSRLLACYGLLAECARSCAAILLDTSLRPQETSPDAAAVNSAVQLEAAVRAASEQVVALVAAQGWDAGSLGRLPPGVALPLREAFQRCRASPPPGWPVQAYALIGREDIAATLAPSGLTTCGPAATADNSRHKYLHNQHHDQQQLQPRHDTHSQQPPPPQPPQPPQPPPQQQQQQQQQQQPHDQVAVASIEGTSHSAPSGSSFASLLRHLPGLRTSHPGVEAQQGSLVTPTSITIAGGTVHAGDGESKEEDASIAGGGGSLGNSSPSSLFSFRQLPQFSFAAVTPSTGITPYGPLGRLAIATTRRSLSYIPYGSGCDGHGAGTTPQATGVAGAGSAVVPYRPLPPPPPPRPPPSYLWHMWRPAYTARLQLPSHQLDLGAELEAPRRLEGPAKQFESKHGMEGLTRGVSRLRFGRDSRLRDVISALDSSHPLVLDGLGLHESDPDAPIKQQQQLLAAALRTMSLPLGRGAVALATGRPLPTEPTAAVPSMCLAGLVPDSLAGHTVVNLDLSTAQPAPGGGAAADFTAWPEFHNGAAEGLRLSAGGGESLSRTWLQVTTKPDVPSYTHAGLLMALGLTGHLDRLSWTDLYRYLSDEHDPTTIAVLVGMAAQRRASMDPTVTRMLFLHLPARHPTTFPELELSPLVQAAALMGVGLLYEGSAHRVMAETLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXV6</accession>
    <name>A0ABQ5RXV6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="5">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_005046</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07011">
        <property type="entry name" value="cupin_PMI_type_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.441.10">
        <property type="entry name" value="Phosphomannose Isomerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016305">
        <property type="entry name" value="Mannose-6-P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018050">
        <property type="entry name" value="Pmannose_isomerase-type1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046457">
        <property type="entry name" value="PMI_typeI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046458">
        <property type="entry name" value="PMI_typeI_hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00218">
        <property type="entry name" value="manA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309:SF0">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20511">
        <property type="entry name" value="PMI_typeI_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20512">
        <property type="entry name" value="PMI_typeI_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001480">
        <property type="entry name" value="Mannose-6-phosphate_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00714">
        <property type="entry name" value="MAN6PISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00965">
        <property type="entry name" value="PMI_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00966">
        <property type="entry name" value="PMI_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphomannose isomerase type I catalytic" evidence="9">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I helical insertion" evidence="10">
        <location>
            <begin position="188"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI62427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="450" mass="48219" checksum="D33B2282DA91C0AB" modified="2025-10-08" version="1">MLRLSCAAQNYDWGRKADASEVAQLAKANGNAIDESKPFAELWMGAHPNCPSKLMSTGESLQACLDKYPQLLGDQVVQNFGKQLPYLFKVLSVAKALSIQSHPDKALAERLHNEHPKLYADPNHKPEMALALSDFEALCGFASTAELQARVEERLKRVPELAELVSQPHVEALLALPATAEDDATLAAAKHALKAAFTAVMTASQDAVTAAVRALVARLKALPATELSEHEALALRLNDQFPDDVGLMSAFFLNLVRLPNGHAIYLPANEPHAYLAGELVECMAASDNVIRAGLTPKFKHADVLCESLTYRQGLPDVLTGSPAGIGITVYQPPFEEFEIHSIDCGTETTGFKLALPASEGPRILLVQSGEAEAAVSGHLPDALEAVPELERAVVLRRGVIVLLAPGVGLSLVHAKDLVIWVAAVNSTFFRLHDARQAANVTEAPAAEALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXW0</accession>
    <name>A0ABQ5RXW0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046614076" evidence="1">
        <location>
            <begin position="25"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="273" mass="29324" checksum="5CD6DDE3FECC2AF3" modified="2025-10-08" version="1" fragment="single">MQLLTEYVLVSFSACLLFLRVASGGGTATVSTTQDLLAAIRNPSVNTIFLNDTLVLDGSIWNTSAGVSAIITRSLTLTAGPNHLFSKTYVLLDFNNLDALFSLAPGCLLRFVGLEIRNHLERSGTFIKPLRQSVGAFVEFRSCVLRRRAGFPAAAEVINLLATHRPETPDGGRTVQECVVHQNLSYSTTGSPARVDVAEAVWLGNFATVVAEDSSLAFQGHQYGGYTYIAVQSYDVADSVVPQSCLDTAPGDTCLFILLQQLEPPPLPSSPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXY7</accession>
    <name>A0ABQ5RXY7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTP synthase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="108" mass="11613" checksum="7564B124C4AE00F8" modified="2025-10-08" version="1" fragment="single">VLVTGGVVSGLGKGVTASSIGVLLKNCGYRVTSIKIDPYLNVDAGTMSPFEHGEVFVLDDGGEADLDLGNYERFLDITLTRDSNITTGKIYQAVIERERRGDYLGKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY93</accession>
    <name>A0ABQ5RY93_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nitroreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_004838</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nitroreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02135">
        <property type="entry name" value="YdjA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052530">
        <property type="entry name" value="NAD(P)H_nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026021">
        <property type="entry name" value="YdjA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43821">
        <property type="entry name" value="NAD(P)H NITROREDUCTASE YDJA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43821:SF1">
        <property type="entry name" value="NAD(P)H NITROREDUCTASE YDJA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Nitroreductase" evidence="10">
        <location>
            <begin position="630"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="121"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="263"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="374"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="481"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="534"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="137"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="382"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="436"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="544"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI62243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="822" mass="80817" checksum="570F0007A390AAE0" modified="2025-10-08" version="1">MASEGTEYIKDRQFYGMERRAAGASITAELAILMLLLVSGASGLAASNVAVTVTMFLGGLTTWLFFTLPEYIQAQQQAKMAMLRIAQAAVPAALPAPLPAALPAAAPAALPAAVPAAPDVPANKESGGGAAGKGAKPKLEAAKRAESKDAATPANDGTVTKESGSGTAPKGPKPTSEAAAAAAAAAAAVAAAAVAAAAAMAEPKPSPAATPQARDGAASRESSDGGASNGSVTKPKSEEAAAAAAVAAAAVAAAAALAEPIPDAMPQAPEGGKKKGGGGGGAPNGGNGAKPKSEAAAAAAAVAAAAMAAAAEMAEPKFTPQAPEGGKKKGGGGGGAPNGGNGAKPKSEAAAAAAAVAAAAMAAAAEMAETTFTPHASEGGKNKGGSGSGAQNGGNGAKPKSEAAAAAAAVAAAAMAAAAEMAEPKFTPQAPEGGKNKGGGGSGAQNGGNGAKPKSEAAAAAAAVAAAAMAAAAEMAETTFTPQAPEGGKNKGGGGSGAQNGGNGAKPKSEAAAAAAAVAAAAMAAAAEMAEPKFTPQAPEGGKNKGGGSSGAQNGGNGAKPKSEAAAAAAAVAAAAMAAAAEMAEPKPAPTSTPSFYDFPGFMTFGDEATASEVVAAAAVPAPAEVLSLIKRRRSVYPKDYTGQKVPREMLELMLEAANWAPTHGQTEPWRFVVLQGEAKRDLEDLTMELCRVRLPEERAVKTLERLQKKRESTWAKISCHIAICCKRQARPEKLMPEWEEMAAVACAVQNMALMGTSLGLGAYWTSWQEVAREAPEMKYFLGLTPDDRVMGFFTVGCVERERLAGYRASRGPWVDKVTWLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYB0</accession>
    <name>A0ABQ5RYB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF218 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055323">
        <property type="entry name" value="C57A10.07/YOR238W"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28110">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28110:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="37659" checksum="433F61D3ABA0888D" modified="2025-10-08" version="1">MLPVYVGHESVHSRHPRGAAGFTDPQESTQYGYSQPQAKLSSVQRRWVSHVVRTALVLLLLAPGWYFLRGRESPQSAALKRNRKLLESEAKSLVPAAALKNLVLVAGHAVYTGVDYSEANKESSWFLEEYQKVKGEAQSFLDHIQLGIEEAALDPDALLLFSGGQTRRAAGPRSEGLSYWVVAEAAGWFGHSEVRNRTFTEEHARDSFENLLFSLCRFYELTGHYPDTITVVSYTLKEARFRTLHRQAVRWPAEYFRFVGTPVPPDAVGAKEGEARTVSAFAADPYGCGGDLLAKKLRRDPFAVGPIHPSRCPEMRALLAYCGPDMYQRHLPWSLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYH0</accession>
    <name>A0ABQ5RYH0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="444"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="838" mass="89797" checksum="989F034C35826249" modified="2025-10-08" version="1" fragment="single">AHAKIAELEARGISANTMTSATSRAPVVRPRSKASGVIIPAFRSVEEVRAHWGVKLLPMVNLALDLIFLPSTSSLEHRGKVGNGVHADRVHLADGSSSYESSNANSSAQDPRAPVAELPFESIADAEAAVAAMIGNLPVLSCARRVGPTVPANAKAGTVPLAQMAHHLPSFPSEDRAVDNGEGQARGANAPQASKSVGSAFSSCDEPSETSCKNDLVPASPGTSPETAPSSRDLTISQSVSDSGQQGSAGSGGGAGASGIPAQQMSPPSPRTPQQAVSLSAVSAGPWQPSVAKSAAAQPPPGFTKKLSPQHVLSLIRERSSGAAGAQRQGSDPSDQRRSGVAHLDSNSAGDKQGVGINDVCYPPGHPYPFAITYQTIDPVWWGCDLKFVNYVRFQKELVLLQQLRQQELYQQQRRHLYNYQLRIQQRVQAQQRRQSPLPNQSRPTATPALPPTSTMHLPAEPLHPPQGPRPCPSFEPDLKDIEAVLVCELTSLFRKEPELFRNVYGFDLDWWIDARGRPLGDAGVPFHNFAAVVVRMDRCAAPAWAGHPQLLNPTVSALLNDKVFGNALILKFLKLDRPAGVNAVLGGKGALYVLPLTPPQLHAYVQESDNVTWQVNCYKNLAPGLVESLATDERATWLSQLPLVPLDEQGNPDLREAVAMIKNAFRIQKPAALTRLHSYSEVMQQRPGGCGYAGAVTSLECQRGGPDAAGGEELGVGIPKEVAPLLRALRCTSPWCTRICGRKPATQHNRTRSCPLGHFWRDLDLSEGSRTPRLVFDAARLCGSRPLQPELCPALVDRLPQMNWSVALPDKGDAIQDLGLQITRNGSSVARLTPNTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYQ7</accession>
    <name>A0ABQ5RYQ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023862">
        <property type="entry name" value="CHP03960_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018768">
        <property type="entry name" value="DUF2344"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045784">
        <property type="entry name" value="Radical_SAM_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03960">
        <property type="entry name" value="rSAM_fuse_unch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03936">
        <property type="entry name" value="sam_1_link_chp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731:SF1">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731">
        <property type="entry name" value="SLL1084 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10105">
        <property type="entry name" value="DUF2344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19864">
        <property type="entry name" value="Radical_SAM_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="432"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1165" mass="127462" checksum="22AF5CD6C10E742E" modified="2025-10-08" version="1">METWCSSRSVASWATGPAQTTRMAARDYHLSPAEYCHRGRMLASGAAVNVAFSHSTIATSACSSASSAAKSGFTSPRYPCSRGLATPTATASVSVVARRAGDKTASSPPHSTTRGHVVVPRAAVATRPGEADPLVPPAGGSRAREGTTPAAAEMKALMAQLQELPEGGADSYIYGINRSEWLKLQGPSRYLGNEVGAVHKPWHSAAVRFCLTYPEIYEIGASNLGHIILYTVLNQSADLLCDRSYFPAADLADLLALHQKPLFAVESRRPLSSFHVLGFSLAYELGGTNVLEMLHRSGIPISWRERCEPDDHPWSPEAGSWPLVFAGGPTATSNPEPFADFFDFFALGDGEEVLVEIGQCLQRCVADGLDRRSTLLRLASQVEGVYVPQFYEAPQGFGGSVVPVVEGVPHRIKRRTCQPDPFQQIGLVPYVETVHDRMTVEIRRGCTRGCRFCQPGMLTRPARDVDPERVVEAVSDGLRKTGYKEFSLLSLSCSDYLALPAVGLSIKNRMQDEGVVLSLPSQRVDRFDENIAAIIGSGQGRKSGLTFAPEAGTQRLRDIINKGLTNAELLRGVRTAWEAGWRQVKLYFMIGLPGETDEDVMGIVETIEWLQRECRTGKWHLAVNVTISNFTPKPHTPFQWHSVSTSEFARKQAVLREALSKLPQVKANFTSIRTSAMEDFVGRGDRRVAQVVRRAWELGATNDSWWENEDRAYAAWSQAIEEAGMSWKYRQVEAGEWDVLEALGDARFRLQGGGGRGRLDRGELRDSRLDLPLPWDHVDTGIAKWWLKADLQRALEAATVPDCSHSGLCSECGVCEEGAGFGANLVAPAPPIPKLQENYKPRTAKVQRLRLRFSKGGDLVFVGHLDLLRAIDRAMRRAALPVASDKSPFHSRPLLSVAQPLPLGATSSCELLEVHLAHKVELQDVRNRLQAHLPPGMKLADEVEECEIYKADGARSESMAKLTSSIEWYIAVQQVDARTAANSSSVDNATATVDFKQAVAGVLALGSYNIQRRTVKKQKLVTENMRAQLLSLEFCQDPLSTPLAEYAPSLLGHLATQQLRGEGGPWGVLKFTAGKKGDNNVLSPERVVDMLASVAGAKLELVHCHRSAINLLKPSPAAVSDTQQQILRSLLRWEGHVALKKHFGQGPWAVGLEHRAARDDTAVMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ09</accession>
    <name>A0ABQ5RZ09_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_005114</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20405">
        <property type="entry name" value="Tudor_Agenet_AtDUF_rpt1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="414"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="746"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="175"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="294"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1250" mass="141203" checksum="9B82FD99AA966839" modified="2025-10-08" version="1">MESYCFTKNQLVEVFGDELDGFACCWIAAVYIEPVGDKQALVQYLEFIGDTDDQPVRETIGRERIRPKPPPQLAFASIQDAKEAHLVERYCDGCWWYATVYSVSAAGVKLSMHECRGQYKVVDNHRLLRPRMEFVRGSWRHAVAKREQLQKLRFDAKTIDAAIMAMKSVKPVPAGPPLFQHSAGGSAAVPTSDPPKTATDARVAFRPRVGSKRGRQPGVENDDQPDAGSAGTVPSDSNTESDEEEPLLKRPRREGKNRVIYVDGFPVLKENMYGLHDDNTMRWQRAFKNGEASLEGVELQPRQRRRSKPKQAPKPVARQRPPELALRLEHNASVRKDSQASEVYRAQYLLRHLDKLEPFITDQVVKRLRLVAENGASLAASKKQGQEASTVTQQPQEILATLRDYQLEGVAWLVAQYERGVNPILADEMGLGKTLQTITFLAHLKFVCRVEGPHLVVVPLSVLSSWLSEFKKWCPAMRVVRLHASDPDERQRLVRDVLGKPTSFDVAVTTYEMVNSQHFGASLKHHLMWRYLVLDEGHKVKNENTHVSCGMRHIRRQQVLMLTGTPVQNNLHELFALLNFMYPDIFTDASKFDEAFDLSKSKVDQKHLEAAHYLLRPFMLRRLKEEVDVRLPPKLETRIQCPLVEMQTFWYRRLLLRNSKLLQEIEAADVQAAKLAEKQQQPKQQLGISESAASPSTKDAWKKVMNLLMQLRKVCNHPFMFKDAEPNFDGETVPEELVTGSGKMMVLDRMLKRLNERGHRVVLFSQFNSMLDVIEDYLVSMGFNYRRLDGSTNRIQRMIDLEQFNLPGSNIFIYILCTRAGGLGVNLQTADTCILFDSDWNPQWDLQAMARVHRIGQTRPVHVYRLVTGGTVEDRIQRRAEAKLYLDQMVNRGSTSNAEQLEGLTRSEVLSMLKFGADRIFRNEQGVMPTDGELDAIMDRSRMMTDAGAAGNAAAAAAESPTAKLDIEAEDNGGDKPDGISAVAGNDATGNAAAAAEVPCGTRLVEKKHTALEFNAEAPPVDTFVFQGLNFRDFLAREASSSLGDIASDFWGKKQRERRERLVDIDGYKVLRDNNYTMEEGMLQFPGQQRGKEAKDMTRGRQRAGIDYDHSDYCQVCWGGGELLCCDHCPAVFHLKCLGLKKRDIGSQQWSCPHHTCATCTRNTRKAGGLLFRCEMCPNAYCEDDLPADYQMVGSCQFFQALGQHHPRQACFIHCSEACKQLYEEMKPEIKEMIEDAAAARRKVAVDARH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ95</accession>
    <name>A0ABQ5RZ95_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoglycerate mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF44">
        <property type="entry name" value="PHOSPHATASE C1620.13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="79"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="403"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="62424" checksum="B214502A4B44DC76" modified="2025-10-08" version="1">MGKNLKRQNDAVLGTPGGASRQGGKPPLSVGGKRHKQMSLVPFLRTPAHVEETPALQVKAGKSGISSTGLQKLGQLKLRTQQKQQPNPTAAPTQGRPAVQVESQWKDASPASDDDFVISMSQTYERTQPEPCCDYGTTEHEEVELSLRRTQQYHAAGATQHYNQHHHELLIATAVAALDSCETVVVTSTTDACEASVAQTSALGANSMSGATIRFPMESILEGQGSSQPGHLAAIAVAVVEGLRAGSRTGPRPSFDFHAHLQAVLPAGSSGPAHTPELQGGGAAGTSAYGTSTEDIADDGTTPPLQIGLPLFPIFTQGRARRRPIHLIRHGESEYNLACKGRGGFGDPNDIFDARLTPAGEKQAKALRPQLLDLMQQHGDPLFIVSPLSRAIETFLHMLPDPERLRICPQTPPPPGSRSSSSQQQQHQQHGQSQPHSRLQAGEAPGAGSSCILGLASKPVNVVICPFLTEFLLTSGDVGRPRSVLIESYPQVAEQLRKGLDKERWWYENASKGPNCAMSKVLNSTEPDKSAKARVDRFRHFVFSQSHRPVVVVGHANFFKTLTHDSHYMSNCQMMTWAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZN2</accession>
    <name>A0ABQ5RZN2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005791</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047051790" evidence="2">
        <location>
            <begin position="23"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="784"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="960"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="585"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="784"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1251" mass="132172" checksum="9FDA887CAD6C037C" modified="2025-10-08" version="1">MARSSLLLVALVGLAALRAAVAQYPPPARGMLKWCSINNRIVNAWACNLASNTPTACETSDLWATDGAECAVPFTGQPVAAETFKFQDTCRYQVARVPLVYSTTRAQGAVLIIFKDYSDNIYFTVQLDGTARVSGQADGQWLYTEPSIPGAGTPSAAIYFWDSPPDTASPITSQAQLVNLMTEDRNSFKRWSCFTYRASTNNICAPGYQLTSLGACNPAVSNPPPVSSKSLAATASANLYISVTVNVVKFGVSTLGSADTGFYCGDPRPDLPQRIATLTLNTTNNVLRLSDIPGFDLPPNCAITQRPPSPPLPPSPPPAPPSPPPPSPPPPPPSPPPSPLPPSPRPPVPPSPMPPAPPTTLFTASLFIYHPGRPTPFNDQDVAKVVTLMRFALGCGGLSNDFSKCQKSPPYLTVNETNNAAFPLYTMLGVKMFFDGFYDGNGNLNPVTDRLRLAEDSVKHMFNTLGTREIWAQLAADQSLGGLFLYCNAYMSLETIINGTAYQQPGFVDDGNYTNVGARYVCPAIQGGIGGPNWPNTTIPPFIPDDIVGMPGYVRLGKIPSNPGYRRRMCDYEALSQPYPVQCLAPPPSPPPSPSPPPLPPSPPPLPPSPPLPPSPPPQPSSPSPPPPSPDTQFCSVRFSVVSTSPRSGNRVGDCSTLSTLVANLFTQFVTIRQPGLFNCVSSTSTELAVSADLLNVASTQQFLYNLGSNSALYNIIATGLNLICGDFFNATANCTAGTVPLSPIVILQPSGVNVTVSYPFVLRPGDLLFPNSVPATYACPPPPPAPPVPPSPAPPAPPPAPPLPPQPLPPPSPNPPPPRPPPGFTFQMSVINGDINDAITNCGRYTTWMNAMLASFERTGNIRRLNPDAPYCSRPAAETILKPELASPQDVNFLYVYLTVPGSIGVFVRDGGVPCGSIVRLYNPTGGVFLDYKCSSNATELVVTDLCCPAPPSPPPLPPAPPPPVPPSPPKPVLSPPPPNPPPPSPPPPAPPPPSPPPPNPPPPSPPPPSPPVPLAPSPPPPTPPPPSPPPPTPPPPSPPPPAPRASPPPKVNPPPPISSPPPPNRRPPWPPLPAKNAALPPPPPYVNITWNPPAGLEIRTYEIFAPFTAVGNKGTLVTERTTVYWLCPALKAALADQLGIPISFKPHPQGIACSDYYTLPKAKGVYYRVTVAAQSEGHLALLDSIQNSGDFVFQSNMVCGSQIRVGLGEPIRGKIVPVAPSPFDPKSLVEPYVYLLGGKGSLGFCLNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S084</accession>
    <name>A0ABQ5S084_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aldose 1-epimerase</fullName>
            <ecNumber evidence="5">5.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_005971</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>alpha-D-glucose = beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:10264"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="5.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.3" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09019">
        <property type="entry name" value="galactose_mutarotase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015443">
        <property type="entry name" value="Aldose_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047215">
        <property type="entry name" value="Galactose_mutarotase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008277">
        <property type="entry name" value="PRK11055.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF0">
        <property type="entry name" value="GALACTOSE MUTAROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005096">
        <property type="entry name" value="GALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI63069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="393" mass="42133" checksum="6426BD8392446EC4" modified="2025-10-08" version="1">MKVLAGVLLGSSMALAALTLLRLRAKRDQSWHSGLPVFKLKNNDGMEVHISVFGAAILKVLVPDKRGSRADVVLGYGTVEEYERASPCTYFGVVVGRVANRIANARFTLDGTEYPLLANDGPNSLHGGLKGLHKRVWEGRLIHGEDFEVVQLHYTSPGGEEGFPGTLHVSVTYLLSRDANELTTRIVATTDEATPVNIAQHSYFNLGGHSSGSILGHKLRLLGADHFTPVSTALVPTGEIAPVEGTPFDFTAADPETSLPAHSIGSRIEQVPGAAPGGYDHNFVLFGMGPQARILTKNGMASEKPKLAATLVDPVSGRAMDLFTTAPGVQFYSGNFLDGTSVGKGGVKYGKHAGLCLETQGFPNAINEPAFPSVVLQPQEKYEHEIVYRFYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0F7</accession>
    <name>A0ABQ5S0F7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_005529</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22582">
        <property type="entry name" value="BRcat_RBR_unk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22584">
        <property type="entry name" value="Rcat_RBR_unk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="277"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="281"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="558"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="605"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="718"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="777"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="871"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="995"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1212"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1267"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1322"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1407"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1465"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1605"/>
            <end position="1657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1686"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1745"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1836"/>
            <end position="1888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2063"/>
            <end position="2088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2168"/>
            <end position="2213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="558"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="718"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="793"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="883"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1002"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1078"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1221"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1322"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1351"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1465"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1620"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1836"/>
            <end position="1847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="2199"/>
            <end position="2213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI62782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2481" mass="260253" checksum="80197E9323F40667" modified="2025-10-08" version="1">MDGENIELDKLTHKRRRPRRTLNAGNEPESSEDSDPLPLRNFVPLTTPPPRALPAAYFRRPELRAWRQGFRPVLRYVPSPALPMRQVIVIDDGEEPPALAGAGSTAHAVEEPQRMTGPGFVGGPDAALDGAPIAPATGPTDLVLGTVARKRVETIQTRRTILVKTNGKGAAVIGEGRSVAAGVAADADPSSQATECPSAAGASGTRLGSSAAVPSGGAGIPAALTSGLPTATLTAGAARMAAVAAPMVAPAAMAIEAPAAAEGGASAAGSVARPVDRQLTCGICLDEKPSHAFHTIESCRHQYCRDCLGRHVQLAAREKVFPVRCPQPHCRGTGVSVAEGLQLLDTAEDRDKLMMSEVESSLPQHLRFYCPNPLCSLLMMLDEAEPAENTPARCPGCHKPLCARCRVLWHSGLSCAQHRALQSRPGGGDEAALLGVAGEHGWKPCPQCRHMVELAHGCNHITCKCGAEWCYKCGAPWRRVQQPTLLPGPAGAFLRGRSVPTCSCQLWDDNNRRLEQEFRARFYARQGVPDDVARRRAVDEVADVGIMLRNLQRIQQRNAPNQEQTQHEQNQIPQLPVAAAGPGGDVDGRLGFERMYRNMRLGLGLEMPDPEAAQPNTNSDHRRADAGGGTAAAVEPAAEAANGEGNSGRVRSDRVLDVRSRAAEAALPLGLGLGPALFGPKGARLRGRRMHLQTQKQVRQDTQVSALAIMPLWVPRHAADGADDGKVEGEGKQAREGDASGGSGAAAAGGSAIRDSERKQNHHMAMQGLLRAALSGRATEVERGNRNAGAQGADRKAPGTRPHERIQELRTLLDNVFDDGDVKQLEEGRGGLEGRVPRRRTGSGEAPTWLLWRHPMPRAICKADTVDPVLGLPVLHGQHPHTQEGQAQQTQQPSQQTHQVRQQEPQQQPRAVLPVDPSLVEPLGRCVFRTISQGRDTVHGAGTTMGADKGTGNAHAQSKAEQIVKLGYIRPRPVLWQRLPTFGGLTFQAFAPRDSDGGSGGAAMASAVAAGPTPSAKRLRGLGETESQGGGTREASNAGLAPVSAAGPSLEPGQHQGRRPTDPAAKRRKAVYPPPGYRGAGGLNNPGRHAGGPSGNEGSDKSDDAAEGVAPFHSNNSLPQPITKRRARDRDNDLLGSFSRHLTALCGGAVCRRKAARAAAAPVGQTAPVVQNWNDGGRGGDGAFGHDGCNVAPPLMLQETLLAPMKRCWRTDEQRHKRRSVATGGVRGASAAAACSGPGADAGGDSQSKKRGLAGVSRKECVIERAQQHQKQPIIFPPMQTGSVCDGRTSMQGKLVNLRSRSGAKDSTHRRLRHFSCFSSSTSATSLSYSSDTEGIGNNYNGNAPDHAGDDTDEDDSGDDGWSSEDEVEQELGNRTSSEPFAAITAAPVPALAMAPPPALAYPDKFQRRLSESRSKPTVAGAAERTAQSPQLRGVAAVAHLPFRAPEAHDSRKGDLRARAIGQCAAPGGSAYGSGASQQQQRQEEEGESEAQRKRQGKGSALAAHDQPKLGLESSLKELSAIMLRMRDEGHWLQVREDLKSMAESAARDATEAVRARERQLQRDTVRGTAGLASGAEAAAAAASSAATASTAVWAMAIGNRGRANAGTMASGRLGSGADSDSKASKRHEGTSADERLAERRSFRAATEGTQAARTKVQVDGQVASWRLGPGPKGTDLLLLAECQLSGQKRKRSPGTASGPDQVKEQTGAALARTATAVREYTQTEVVKEAPAPGRGVNVDNRLSAAQPAAGSGRPEGRNLGSASGEQPREYTGSMRSLPAQAGNVSCRQPEEPLARGWGNDELSNGDMDGGRGRILLRNDLDQPLFLRHNFRMAREQQLRHQREQKQHRAGPAHGSSQITGTPHDPPFAGLDPSEVVTGPSPANEPGITLEEEIDANGRSTMPLGCEPSIMAGDLEAPRPFLETAASHPANDRPTYAIRSGFDNVSNLTTDILRLAGTEYPIGRNGSDSRSHGASQRTKVAAEATLTGHESVRCDQTQSPFAAAATAGKSLRDRYPCKSFGLRTSRRVGAETERDTDGIEVTRQPTSAPVCKTTEANSAMAEAAGGSRLRSAPAHRRGSRRQGAEGDSIPHPSCHALLPEDCAVQALGAVDAGNGIAVDETPPYTGDARGLRQMPGYAGSLRMNLIPDTMLSALGLGPGPVDDMAVRTAQPSGEERRSGSFTRCSRRRRHGGDCDIFPSSAAGGSGNAADRSSALRGNRGVMAVLGGPLDSMTLAKAPGRDVLDGGEEGIMILTAATTAASPPNDAGVAIALPVRAPGEGAQEAKLQLPIAALPGLLMGPHGRDGEVDAEDIPPADTVMAESGAIADGRPETTTAAKKPAGIIPGQLPGLLMAGGVGASNLTPVAEAAQVSGPPIVGLQILPAVMMQVPADVGGAVGARGPTAASAAVATTATGVATSSDQGPQVAPTPPPIVVQVVLQMIHQGNEPLLQETLEQIRQQLDSLLAGVRQQQRSGHVTEPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Y9</accession>
    <name>A0ABQ5S0Y9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial glycoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006376</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="28250" checksum="433F07250DD77D97" modified="2025-10-08" version="1">MATRQRLARGICCFFAQQQSIALTVQHATGAMISAAQQSALAAHHHQAAPLLLRTAVAAFSRSLTTGAPSYNSSLSSLLREEVDYEKKNYVRPDQVSGGPPAPFKLTESPGDTLLSLSRTYKNEEISIDLHVNNQPSPPYEGEEGVDEEGITTVAFNVSVLKDGKMLVFECESDGSSVNINHVSLEPKEGLTSESMYSGPVFDELDDSLQRNFSTFLEERGITAELGEYLRFLIYDKEQREYQTWLSEVEKFVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S155</accession>
    <name>A0ABQ5S155_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF819 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008537">
        <property type="entry name" value="DUF819"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34289:SF8">
        <property type="entry name" value="DUF819 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34289">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF819)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05684">
        <property type="entry name" value="DUF819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="48544" checksum="59846FB13C7675B1" modified="2025-10-08" version="1" fragment="single">YSLRIILLSLLPNFSTSKLLVASLLLAPRASIDISLTTIDLPFIAFFAFLAPIHCRSGFWGFKAQNFVRHLALYSHPIHDITVLLVLICESRTRWGAALSAPLVSMLCAAVLAAGGLIPVECPAYDSVWRYLMPLAAACFLLETDVRNLVSNGGPVLAAFAVGAVGMVVGALAGWWLLREALGPVGAKLVPCLCASYVGGSVNFAAVALAVQVPAAYLPGAMAADNLMMAAFLAVLMAVPVQKAAVAVAGTGSSAVAAPTGASGKGDGESVAVMQMAVGGGGAAAVAPPAPAVSAESLSLTLAVAAAACTAAHGLAEVLNVVPLTLLILAAVATAMAAAAQWLGGAGAATVAGSERGSKAAAAAVCKGPIFAGASQVGTSLMMLFFAVIGASAGSPSCLAGCGVLVPFLGLMVGVHWAVVVAVGRGVLRLPAEALLIGSNANIGGSATAAAMAAAKGWRPLLQPAMMAGSLGYAAGTAAGLLVAKIIGCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1E8</accession>
    <name>A0ABQ5S1E8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="138" mass="15412" checksum="4C47F4FB2DE2C2B4" modified="2025-10-08" version="1" fragment="single">KSLAVIGQVKAQLLRAFAMRDLGETRFYLGFEIERDRAAWTILVTQRRYISNILEKFGMKGSNSRATPMDANLRLTTEGDPLDTSVHPYSEVVGSLLYLAVCSRPDISFAVGSLARFMAKPTINHWNAAKGVLRYLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1L4</accession>
    <name>A0ABQ5S1L4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cation-transporting P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006265</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="863"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="985"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="10">
        <location>
            <begin position="10"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="472"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1065" mass="114416" checksum="A7B160C612141DDE" modified="2025-10-08" version="1">MAASPEDGYAPWSKSRDDVLKHYDVNVKEGLTDDKVATQRARYGFNELEKEAKQSIWAMIAEQFEDTLVRILLLAAIVSFGLAWFEEDSYEEGIRAFIEPLVILLILVLNAAVGVWQENNAESALEALKELQTETAHVTRNGKLVSDLPARELLPGDIVHLHVGDKVPADCRVVALKTATVRAEQASLTGESVAVNKDTEPVADPNCELQAKECMVFAGTAIANGSCTALVTAIGMATEIGKIQAQISEAAKEDDETPLKKKLNEFGELLAKVIAAICVVVWLINYHHFLNITFKPEGGMPTFSFNLSKATYYFKIAVALAVAAIPEGLPAVITTCLALGTRTMAKKNAIVRKLPSVETLGCTTVICSDKTGTLTTNQMSAVALTAFAAPDGSSLRRWVVAGHTYNPDEGEVEGLAPGAGLDKALQAIAEVCAACSEANLEFKGSAFRAVGAPTEAALLVLAEKLGLASPSETAAARKKRTATPEEHPQPVTQTYRERAPVSATLEFDRDRKSMSVLVRPTGASKNTLLVKGAAECVIERSTRMMLPDGRIVPLTTAARAAILGAVRDMARDALRCLALAVKPDVPSPLADYDGNSHHPSMKLLKDPASYAGVESDLVLVGLAGLQDPPRPEVRPAIDSCKQAGIRVMVITGDNKDTAEAICTKIGVFQPGDDLSRVSFTGRAFVELPRARQLELLAAAPSMCFSRAEPRHKQDIVRLLKDQGEVTAMTGDGVNDAPALKLADIGVAMGIAGTEVAKEASDMVLADDNFSSIVAAVSEGRAIYNNMKAFIRYMISSNIGEVASIFLTAALGLPEGLIPVQLLWVNLVTDGPPATALGFNPPDKDIMSRPPRRANDQLITPWVFFRYMVIGGYVGVATVGAFVSWYLYDHFAGIDLSRDGHTTVTWHQLSNWQQCREWGNFTAAPYKLAGGGQVSFSHPCDYFSVGKAKASTLSLSVIVAIEMFNALNALSEDGSLLAMPPWSNPWLLAAISVSVALHCVILYVPFLADVFAIVPLSWPEWQLVLLWSAPVILLDEVLKFIGRIIHAPRALPGSKGSAATRAKKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1T8</accession>
    <name>A0ABQ5S1T8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006386</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="596"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="510"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="518"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="870" mass="92766" checksum="D735E5CC41ECE384" modified="2025-10-08" version="1">MSGFHSKSLRTSHTAQIVLVLLWHGWSIWGGIAVEVGLRSGQRGHVGEPHGRLLASFKVANVSTADDFVAAFVNPNVELIRLTSSVVDVPRSAWDPYRPIILTRNLSIEGNTTAFDDWPTLQLNRATAVVSVANGVMLEFRNMFLDGVLATNPTPDQGFTILAPTPLGPPAFMFFGPGSFIIPACFPAHMQMAMVESTPRPSCLPGNQSAITGLPQDGCVNRTDAPPMRRCYASRGLLVDLASDGVSGDPATYGQKTNFVVWLHDVIGLCQEMASDVCIGTRAPYLCYLELLNRTRPQTSALAPQTSEDQRQQQLSTSSTGPGAQDSTAMQLGFVVEGATEGSAATGPHGGGSGVGCRNVIIVACAVSAGVAVLAVLLGLAVWSRRRGQRGEGTGGSSDVRADGVKINGLSDVEETVDGQPGTEGMLAPNEGPGAHHGRYDMDGTAAALEGASVAAVKHPLPRVALTISDSKDSKTLHAASHTVVLTPNTDPRASLELHLRVVDRTALQAGGKGSTGEEGSSIYVDADSGAGRSGGGNSDGESSGEDNGVGNGPEGGYQGADEHHYSVSSGNNHKDDGEGTGMDGRVGSSMPTVLELLPVIRGKGAFGRVVEGLYREQRVAVKLLLSEGEYGATLEEVFTKSFVQEFQVLSRCRHPNVVRLLAACVDGPRRCLVMELMETSLERLIHSGPLIMPLPKVFHIGICICNALAYLHPTITHRDLKPANVLINNPSSDRPIVKLSDFGTSRLRLTVTPTLDPEAGTPAYMAPECFDLSNNRILHHADMYSLGVLLWEMVTCLRPWQGQGLMDIAIKVTWNRNRLPLNDLPSSRCPQKLVRLLHSCWDADPVRRPAAAEAAKILTLLLQQVERES</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2B6</accession>
    <name>A0ABQ5S2B6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_007162</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="208"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="428" mass="47848" checksum="EC8702FC15166D16" modified="2025-10-08" version="1">MANIDKQIFDDDTEEALPEEFTYMSAEAIQQRVRLLDNELRVLKDESTRLTLEQDTLKDKIKENKEKIKLNNQLPYLVGNIVEVLDIKPEEDEEEDGATVDLDAQRRGKCVVLKTSTRQTIFLPVVGLVDPNTLKPGDLVGVNKDSYLVLDTLPAEYDSRVKAMEVDEKPTEDYSDIGGLDKQIQELVEAIVLPIQHKDRFTKLGIKPPKGVLLYGPPGTGKTLIARAVAAQTNATFLKLAGPQLVQMFIGDGAKMVRDAFALAKEKSPCIIFIDEIDAIGTKRFDSELSGDREVQRTMLELLNQLDGFSSNDDVKVIAATNRADILDPALMRSGRLDRKIEFPHPNEDARAKILRIHSRKMNVASDVNYEELGRSTDDFNAAQLKAVCVEAGMLALRRDGTEVTHEDFVEGITQVQAKKKTNLMYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2P4</accession>
    <name>A0ABQ5S2P4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_006758</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="359"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="638"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="867" mass="88117" checksum="FDF459AB5D7A28F2" modified="2025-10-08" version="1" fragment="single">MERYEYLETISSGAYGVCYRVLDTATGQHCAAKGIKQAHENAEVMRLTLREVKVLKTLPPHVNIVKLLEAFRSHSGRVYLVFEYVERSLYQELERHPSGALPGPMVKSVAFQLLHALGHCHEHGFMHRDVKPANVLLTGYIPGVGSGGVVKLCDFGFARAVSSSGAGRAGGAGAGLGAGAAGLSSYVMTRWYRPPEVLVGDVYDTSIDVWSYGCTLAELATGRPLFPGRSTVDQLWRIMRCLGPLSVQQTACMRRDTRLQPVWTPPQRLKTLRQRLPDLDPRLYEVVSACLTLDPQRRPTIQQLLQMPYFWDVRALIANTDLARQLPYAAEVACTISFRQPMATPAAASAVKQAAIPSGHLNRTDASPPPERETAAATMDDSRTAQLATASAENPKPPKPAVPAAASAVGSRHNDDDDNAAAAATAPVIAQGAFVQFVGDQGLASTQPLILPTQPKQSQQHSHGGVGIASDVLNVSNITSQSSEGTIAATIGTACTDATKGMTTSAATVANDLTAAATGKSGLGSDAAADAAGSGSSGGGCGGGAVSPRVPHSTALTAQMVAASNAANAAAAATVAAAAATAASAAAATNAAVAAAAASAAASAAAAMAIGAYNATHGKAIPNPSQLLPLPINLPRSSVQSAAPRTPARENDLSAMAADQRSGGKASSVAGPYRSAAVAATAVAATAVAAPGDVMQKEEAAVPAPPNSLRVLDAACLYQALSGNGGSELTHGSAASTLPSSDSRAPVIVVTAAAATATDNPQPFAAARLKAAAGGTAGGAATAAATAEMGSAIAGGPLAFQPSSFQYEVHSAVRSGASVSYLGDAVAFTGYNGTDLPQMDSGGLLYLPLGNGEEGISRIKMPMAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2X1</accession>
    <name>A0ABQ5S2X1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">homogentisate 1,2-dioxygenase</fullName>
            <ecNumber evidence="4">1.13.11.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_007266</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the homogentisate dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.5" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07000">
        <property type="entry name" value="cupin_HGO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046451">
        <property type="entry name" value="HgmA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046452">
        <property type="entry name" value="HgmA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005708">
        <property type="entry name" value="Homogentis_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01015">
        <property type="entry name" value="hmgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056:SF0">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04209">
        <property type="entry name" value="HgmA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20510">
        <property type="entry name" value="HgmA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase N-terminal" evidence="10">
        <location>
            <begin position="11"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase C-terminal" evidence="9">
        <location>
            <begin position="284"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI64094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="489" mass="52620" checksum="DB21DE9C3B607C34" modified="2025-10-08" version="1">MAATAASTQLKYQSGFGNEFSSEALPGALPEGQNNPKICPYGLYAEQLSGTHFTAPRVANRRSWLYRIRPSVTHEPFHPVNFPNESLTADFAAGTVTPNQLRWRPFPIPPEPVDWVRGLFTLCGAGRASTKEGFAIHVYAANQSMDNSALANADGDFLIVPQQGALRLKTELGLLDVAPGEIAVIPRGVRFAVALHNGAARGYVLEVFQGHFALPDLGPIGANGLANPRDFLHPVAWYENVEGLSYSVLHKLEGHLFVAAQSFSPFNVVAWHGNYVPYKYDLARFCPMNAVSFDHPDPSIFTVLTVPSYTPGAPPAADFVIFPPRWLVAEHTFRPPYYHRNCASEFMGLIKGQYEAKQDGGFLPGGASLHLCMTPHGPDTATFERAAGRADLAAAEGPAHLGHETLAFMFETTAIPRITPAALSCPCVDRDYYKCWVGLKSHFDPTSGPNATSYPKAQEAGAAKAIGSTAAADGTAYLGSGSLGTMQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S425</accession>
    <name>A0ABQ5S425_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial transcription termination factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007973</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF112">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="237"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="29131" checksum="8A2E480715E9E17A" modified="2025-10-08" version="1">MHHLRLRSARYRVTSANSLALLPYRLACLHSSRHGPATRTERGKMRTICNANKEIDSLEVQPVRRAFLSVGVSADDLTRASRLEPALLNYTTDRLHDIIDLLLNLGLTGSDIGKVLIAFPQAFQLSLDHHAQPVVEFLRRDVGLGPSDVRALITRFPSILGMNVKGQLRPQVAYLASLGFSPETLPELVLSRPLVLGPGIETVISFLRRCGVPRSQMHRLLRSYPLDYRVHLKGYSAATPNRGSSSSGGSPPGTGGGSGGGRGMGRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S473</accession>
    <name>A0ABQ5S473_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vacuolar cation/proton exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_007630</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Vacuolar cation/proton exchanger (CAX). Translocates Ca(2+) and other metal ions into vacuoles using the proton gradient formed by H(+)-ATPase and H(+)-pyrophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. Cation/proton exchanger (CAX) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004798">
        <property type="entry name" value="CAX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00846">
        <property type="entry name" value="caca2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00378">
        <property type="entry name" value="cax"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF22">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0050">Antiport</keyword>
    <keyword evidence="7 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 11" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="9 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="12">
        <location>
            <begin position="124"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="12">
        <location>
            <begin position="315"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI64419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="460" mass="49102" checksum="F6140D04B41A375F" modified="2025-10-08" version="1">MSAQRKNAITVVKAETRQRLAELDDVDGFDESLLNNYGNGAGPVEGAQFARVSAATSLGRGGHAALAGADRTLSRMSVLSVAPKWFVRDYLATKEIILSSWFNLLLICVPLGWASAFLGWGSIPTFTLNFLSLIPLALILGDITEDLAVRFGDIVGGLINATFGNVVEIMVSLFALFKNLFTVVAASLLGSILSNLLLVLGCSFFLGGLFHSVQTFNAVSNRACSSLLMLACIGISIPSAASMILRNANSPIPAPDADPLDPEVHERWILEVSRGTAIILLICYMCYLAFQLKTHSNLFKGDDDDAVPMLTLGTSIGLLTVITAVVAVCSEFLTDSIKDVSEKTHLSEAFIGLIILPIAGNACEHLTACIVAMRNKMDLSMGVAVGSSIQIALFAIPFNVVVGWATGHPFSLDFTPFAALVLVVCVVNANFVTADATSHWLLGVQLIALYCLVSVAYFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4A4</accession>
    <name>A0ABQ5S4A4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="798"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="553"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="846" mass="86099" checksum="0C7C6D923AF2824A" modified="2025-10-08" version="1">MYAKPKLGLLLILRPRLHPKPQRIGMIRRPSSFILVNMQAASAKVEPFPVGSEPAGTTAAITSANLSTAAVSSIVTTTRNGRSNGSRDIAGCSHWPRPSKIQPIKPALKSTDGGVAPKTYIPFSVNGTTAPITDQAASRSEATRPQRQRPSQSCPGATSSSHNHRLANESRPNANGGTASIDGPNVNNGTRSMSKGIGASNGSKMRFSNIGANNPHNNSDELDTKCGGREEQEYDALTGGFISEPGEGCGLTSDSGYNPYSDPYDNVTGDVAEQSGLSRATAASSAAYAAEAVGGLQVGGRTSSTAVTSGGPSRSAKSNGSSWSFRVMTDLHLTPDRYGRLRLPSILMFTHRRPPRHARTEAWWTKLRGVLIDTGRSVTESLGYRSRRRVQTGDTEDGSSGGTGGAYGGGSNSGQGAIEEEDCIDCRTSAEAAEGNQPLPGQSRLVESQSRSQPCATATCNGSSGSGGSAVPGGGNGGVRGSGASRKLAALEQASSTRSKGSEVGMMMADVATTVERAAVAGLSAVTSSTGLPGAVTSRESASELGSQSFSRATGSHADGGSSSSRSDAATRPVGDGVATPMYYDPAPLPPMGSSSCCDCGNCSGPVADNPCSCSNSSCSRGNSSPSTRGASKSGGCTRHTTYEGDGCSGGGDGVAAASSASDCYSLAVGVFSKQLPPLGLAFTASEFDEAPPAARTPTEDVRSDFLHIVSADDAAAANADPADQQSPLFSPWLLQDGNATGMVPAAADSKSSVRPLNAESTAGIGLSPVATDGVEDIPSPMSSTYLFYSSIAGVGTTPAAAAPRSSNGVDGGASSRDFTAGVPSLPPLPLRHEPRPRTPPAGKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S503</accession>
    <name>A0ABQ5S503_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_007596</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF7">
        <property type="entry name" value="NIMA-RELATED KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="291"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="551"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="304"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="316"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI64356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="694" mass="74398" checksum="6FE6E6EC7CE13664" modified="2025-10-08" version="1" fragment="single">GTYGKVYKVRSRVDKEIYVVKKVHFDGTPQSEAEAALREGQMLSLLRHPHVVPYKEFFKHPDGDLCLVMAYCEGGDLFRFIRDLRKQSKTVPEPQVWQWLVQLLLSLSYIHSKRILHRDVKTQNIFLSQGKVLIGDFGLAKQLQRTFEMARTPIGTPYYMAPEIYEEQPYSFKSDVWALGCVMYEMMTGKAAFAADNLSRVVLRVIRGQYDPLPESYSAPLRDVVTSMLCRDVKSRPDANQLLTVAPVVSHVQSYLETLTAEGPSGWATWRMKLPIAAMVQVSEVLEAAGVVPTTPGGSRRPSRPNSAHSFSGTSAPAPAAAPAPAVLKPEPPLALEPSLAPEPPAPAPQTPDTRPGDAGGQVVKLPPLPGAKLQPIRTPYGLATPFMGAVVQPQQQYAELRVLAHQVMAIRTTQCGDGAPEVTTPAPASASAVAAVADGDGAADVQSSPKGADEVVKALDRTLIRTLEIARKAAADSIPAAAAGRKGKAGGETGVVIDEAWLSAGLPQGGFGFGHSNSQKDLASADVTLPPIRAPRSSSSQCQIQWGAAAGRAPAGMGAGPTSPKPQPLLSQQQPSAVTAGATEPLTPSPGFPVRAGKYEAVDEGFVSLTQVSLHAQSLSNRRANLKRYCEERLGDELMRQVMKAVFMAQKSSRDGRDVAVTEAALRQQLDRLLGGELAEMGPLVEEMALLHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5A0</accession>
    <name>A0ABQ5S5A0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007771</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004790">
        <property type="entry name" value="PRK06136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF3">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT UROPORPHYRINOGEN III METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="6">
        <location>
            <begin position="56"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="288" mass="30190" checksum="4C5D84EE7FFD3B81" modified="2025-10-08" version="1">MLGGQMLAQQSCGKCFGLSTRTAPPAFRSARSRGFIAGASNTSASVVALYSAKLGKCWLVGAGPGPADYLTMKAVRLLQTADVVVYDDLGSQDALGFLSPATELVYVGKRGGRESITQPQINELLVARCAEGRNVVRLKGGCPSVFSRLASEVAALRAAGIPYELVPGVSSALAAPLFAGFPLTHVTLSPSFTVLSGHDPDSIDWGALARLPTLVLLMAGRGLGEIATRLQDTGWPTDTPVVVIRAAGLSQQQVWYSRLDRVKTDTANVESLSPCVVVIGRVAEFTDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5L5</accession>
    <name>A0ABQ5S5L5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_008346</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051412">
        <property type="entry name" value="Formin_Homology_Diaphanous_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003882">
        <property type="entry name" value="Pistil_extensin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691:SF1">
        <property type="entry name" value="FH2 DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45691">
        <property type="entry name" value="PROTEIN DIAPHANOUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01218">
        <property type="entry name" value="PSTLEXTENSIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047207967" description="VWFA domain-containing protein" evidence="6">
        <location>
            <begin position="27"/>
            <end position="2588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="2513"/>
            <end position="2535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="248"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="835"/>
            <end position="1683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2472"/>
            <end position="2507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="256"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="2477"/>
            <end position="2487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2488"/>
            <end position="2500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2588" mass="268816" checksum="34C1B9A376B25A43" modified="2025-10-08" version="1">MQMAAAGTLLPAAGFLLLVLTSVSVAARPAGDGSWAFDKSFVTGRKLAQVTTPAPCIHSDPTNYTYYATDISGPADSCTDQSGCLDFLADSGTCRSVNTTTGAWLYCQFCIFWTDARNCPKDTVDTISHVCSADEFWTPGIILSSGTEPVPKATSKLDGWSAYSNNKYCQWIRFNTTATWVDLAFTVKDGTQACLPANAQPLSANINGISATCQGPRTDSSGTRAGCGTGDQANECLWTFRVPRPGTQGFKCSNISPPPSPPPPPSPPPPRPPPPPKPPNPPPPRPPTPPPLPPGTVLPPSPPPPPLIMTDVGGYSCVSTDDADDTGHCTGTACGRLYANIIKKALDMAPLDASGIIAFGTFTSATPARTSLESWVGLAGYPASYITYATDATKFASYNLTQYKLLYVPSDSSNTVGGITSTQNNALIAMKSSIINFVNKRGGSMVVLTQSTFGSKAFGFLPVSFNFTALDFVDVSVTPEMQLFSPDSNNSNLDHVYYHGYFDRPIDWNGMRVMAYQTGFCPVTSGRLQECRATVLCNTKTILTAENCYDGIDNDGDGLVDKNDPDCWRCGDFFVDPGEQCDDGNILDGDGCSSTCQWQDFPPPPPPLRVPPPPPTNDPGTVNYCSASGTVGCASCQGNCTTVDNWWSNGRVCKLPQALGGAMPNDGLCKNDYGFVATRKVDLYTVSSTGSKTVVGMVDTFRTARGILHVTLRALCPNLFFTTSNTTTTDINHFLLLSFSISGNLTTVSVPLRRNATGTLLYSCYTFSYDLNEIVPDIACAQINLDLRVTARTTQVSSTTGNNCVIQPDKLTQTDTIVEKRNYYNVTPAAPPFPPPPFLPPFPPPPRPPSPPPPPPSPPPFPPPPSPSPPPPPSPPPFPPPPLPPSPLPPNPPPPSPPPPSPLPPSPSPPPPSPPPPSPPSPSPPSPSPPSPAPPSPPPPSPPPPPPPPPPAPPPPSPPPPSPPPPSPLPPSPPPPSPLPPSPPPPPPSPPPPRPPSPTPPSPAPPSPLPPSPPPSPRPPSPLPPSPPPPSPLPPSPPPSPNPPSPPSPPTPPTPPPPLPPPSPPLPPSPPPPSPIPPSPPPPSPPPPSPPPPSPPPPSPPPPLPPSPPPPPPPPSPRPPLPPSPPPPSPPSPKPPSPSPPSPSPPSPPPPSPLPPSPLPPSPPPPPLPPSPSPPSPPPPPPSPPPPSPPPPSPFPPSPPPPLPPSPPPPSPPPPSPPPPSPPSPSPPPSPSPPPPPDPPAPPPVPVPPSPSPPPSPPDPPSPDPPSPPPSPDPPSPPSPDPPSPPPSPLPPSPSPPSPPPPSPPPPSPPPPSPIPPSPPPPSPPSPDPPSPSPPSPEPPSPEPPSPSPPLPPSPSPPSPLPPSPDPPSPLPPSPSPPSPEPPSPEPPLPPSPEPPSPPPPSPYPPSPPPPSPLPPNPPPPSPQPPSPEPPSPPPPPPPPSPPSPPLFPFPPSPSPPPSPPSPSPPSPEPPTPSPPPSPLPPDPPPPSPDPPSPLPPSPPPPSPSPPPPPSPPEPPPPEPPSPPPSPPPSPPPPSPPPPNPPPPSPTPPSPPPPPPPLPPSPEPPSPSPPPPPPPQPPSPSPPLPPSPEPPSPEPPSPPPPPPPPSPPSPEPPAPPPSPLPPSPQPPPSPSPPPPSPPPPDPPSPPSPIPPSPEPPSPEPPSPPPPSPPLLPPPSPKPPPPPPNPPPVPNCDCSKIPPVDATTNACPANNIILRFKEGNNRFNNSLIETKVCVPRFGYDNVTDIYKFAFCWSTSCPVLQHLSNKQFRIYFDNMDLTPALPDNRLAQTPLVVTHLTPECAKPTTIVDLVNNVSTYLISTQQKLLQFPQPGLAAMDMDVNVSAGCNDEWIVFAMVPVHDVGGLTPPIPPAACPCWDTPTNGVCPGIKVPITLTTLDLPTEETYVQCIDPANYDTRTGRMAYSFCWRYACLPPVFATTRFTATCPELERWNIAKMGGNFSLDFQRLEGVQMVAIGHPKNSTIGDIRYDISDDDTVVQLPDGGFGNITFIYTVPPGFDDLSAAITMDAVGPRPPPPPRAPFTGCTCPLVPERTIGGTAGQCNSTYATMTVTFANVTDKPDNFTAFYQCVPWPNYDILLREMAWSHCWAKMCLPKIFENASYNVAINQVTKHNIALMPPAKYQLILFPAPYGMQIYTTLYPVSGSPMEYNSTVGSAVIGGTQQDIDMPALSNLTIRYYIPSRWDVDGLAAATVLRMVKGSEVPRPPLPPAPPPAPPAPPPLPAAVERVQITTDFMLTANISLETLGGGFQYAGKYVTVPVKFYDYQLLPDCTDASIAAYKARVQQALSLNDTSGININCRYAVSTYGQSVRRMLRRMLVSATTTGSWTLRKVGLTSAATTMERRFLQTTTSTTPMMDVSVTYPDTTGVATAVSANQACTNLNNVGGSCDAAPSGLIEAAYVQHTSVLPAASVTDNGCTSSSTAALNALIQSGQVTASQVQVQACTSQVIPAQKAVASSPAPASSPAPAPASSPAPASSPASSPAPQGSSGNNGIPKGGIAGIVIGSVGGVLIVAIVGLVIKNRFDRADTVTIGNARETGPAPRGSRWRTYSMQQRAEQATSGQRPVAGVSVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5R6</accession>
    <name>A0ABQ5S5R6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="20295" checksum="1F429CE4D5066894" modified="2025-10-08" version="1">MCPLSPPSSPPPPTRPSSSDIYISPMLCGQDFGIKYATSSDANPASTFPIQTLNGRAGSVLEYAMSTYDCNGRYQGAPHGGVYAASSPTVTLDLSSQLLDPGLRITKFTACCAGTNSTWANGAQRIIMMTAGGMQLNIGKRCVVPQPWINVPTGYTFGGLVTQSPADEGRNWVHRLGFIAVRYRPGVKVCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S609</accession>
    <name>A0ABQ5S609_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deoxyribodipyrimidine photo-lyase</fullName>
            <ecNumber evidence="3">4.1.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">DNA photolyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_008700</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA).</text>
            <dbReference type="EC" id="4.1.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA photolyase class-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.3" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008148">
        <property type="entry name" value="DNA_photolyase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032673">
        <property type="entry name" value="DNA_photolyase_2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052219">
        <property type="entry name" value="Photolyase_Class-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00591">
        <property type="entry name" value="phr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10211:SF0">
        <property type="entry name" value="DEOXYRIBODIPYRIMIDINE PHOTO-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10211">
        <property type="entry name" value="DEOXYRIBODIPYRIMIDINE PHOTOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01083">
        <property type="entry name" value="DNA_PHOTOLYASES_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01084">
        <property type="entry name" value="DNA_PHOTOLYASES_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="14">
        <location>
            <begin position="126"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI65219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="578" mass="64963" checksum="F5ACFFC690A5D3B1" modified="2025-10-08" version="1" fragment="single">KFLQLSRQQVQALHFRSPLTRPPRLAQPYVRTFPASLSLCITLLTLALHFSAINSPYIRARCCVAVISFRRDIAAAAMSSKRKTDAGTSSPDDGPSAKKTATAAISGTLVNPKRVRELKPGPVGNGPVLLWMSRDQRMNDNWALLHAIEVAQQRKQQVAVAFNLVPAFLGAGARQFGFMLRGLREMQPRLEAKGIAFFLLKGDPSQTLPDLASRLGAGLMVTDYSPLRLGRHWREQVCAAVSVPVHEVDAHNVVPVWVASDKREVGARTLRPKIHKHLPEFLCEFPEVSQLVPWSHAVKPEPVDWDALLEEVLTRGSAVPEVDWCKPGEDAGIQALKGPGGFLSPSRLSLYDTKRNDPATPKALSNLSPYLHFGQLAPQRAALEAAKHRAKFKAAVESFLEELVIRRELSDNFCHYTPNYDSLDCAAEWARESLEKHRVDKREHLYTRAQLEAGRTHDELWNACQLEMVHVGKMHGYMRMYWAKKILEWTGGPEEAIEIAIYLNDRYELDGRDPSGYVGVMWSMAGIHDMGWTERPIFGKIRYMNYNGCKRKFDIKAYCAYVDRLVSEAKAKHRAKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S887</accession>
    <name>A0ABQ5S887_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009850</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF84">
        <property type="entry name" value="ESTERASE_LIPASE_THIOESTERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="354"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="372"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="611" mass="64904" checksum="5E9B5988B699F063" modified="2025-10-08" version="1">MPINQNIAAPVFWEVFAILRIATLALLEAFLGILSWPFVRRKEEVALSYISTEANQRLIDGCPAIKAFSCSPWAVGPHAQTFFSVIKLGASPIPRKELVTMPDGVQIALLWFEPKLVAEDAPLVVSLHGIGGDENSVRPIVLAEECMKRGWRSVLYVRRGHGDSSLLPVAPPPSTGVASPKESCGKAMGPAVTAAGGGHTGATGAACAAAASAPLPSSPLPPAVSTISSDAAATVAALDLSPPTSPPTAVTTVTIIATDGGEVTQTSEASHPETPTGCLSSLQPACIHAAAAPVAKPDLEALRRTRKAFPQHADTEDFHVVLQHIRAARPRALMMAVGFSMGSNVLVKFLGEHPPASSRHTANTSPEGPLNGERDPRGDTERLEEDPKNLLAAAVSVSNGYDLIEGTRHLVSSRPMADRIITAALHKLLRRRLPEVHAICAAHGLLVDFNEVLACNTIRDFERALMLPISGHDDLDEYYRHNNCREALMNVRTPLLCLSARDDPIIDPSLLQYAEDAAASNPHVLLAVTGRGGHLGWLQGWTGKSWMMDVIVQFVESVVQLHAAGGDSDSAKVAVSIEKLRATEALVVSKGNQKTFVEAEEALAHAADAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8X3</accession>
    <name>A0ABQ5S8X3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13361">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13361:SF1">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="883"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="959"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="990"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="960"/>
            <end position="971"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1155" mass="120682" checksum="1AC70D5C82E58758" modified="2025-10-08" version="1">MTAATGRIVGSSRSGNSAMAIPAPMPLGHHAVQHRTIPFGTSWPCASSPPSFPATDLATSTIAVLQRTRGSVGPKIHAPKSVSAYSAPSTSAMFCASFGTATGVANPTVSAAAASSGLTFDNSTGFGTNGNVGGSHRWPAPARSRVRACAVVSAPILSHSPRTAGSVAGGASADPGGSGGTTATASATRPPSPPLGLFISYFDGFCPEGATECSLVWEQLRSCSKWQQMRQVYDNLLRAEAAALTEAQAVEVVHATRDDLARGKSTLGSNTDVSNSSSNNNSSYSGDSSASISMDRMATSGTSSATARNCAPGLLSRDETYAFLLQLQDFFHLQLDQKELEELQAFRCRLVRDLSVYVRRLNAEQLEAMCCASAVSGKCLSRDRADAVQQRLLKILLSTSESGANGNTSYTNGYTNGNGGGSSNEIRRRHQRKLVAGGFQVLTPALNIIFALYDHGYPPQVALLQAVFRQAVDHCGEVTRSQWHGLNGLARRFRFLLDDMWLEDLHAHLREQLSCAATDFGHMASILGCISAFTANKVIEALDTRVLVVVEAIERRCDEGRSLLTAHHLVVFTALLVDIQSPFRAKWLGLCLDHLGSCRSHLSGLAPATLVLAVELVLRGRGWDEQRLLGKLLRQLFATYTRHPAATLRSASSNVSGCPPRAASRIISSSPSPASAPSALPAASTSLSAMAAAASWDVDVGGSGGAASPVLDLAYLVDWLAAKVPLELGPERTDTLEFLQPLVSGLVEDGRSQLASLEPRCLPGLIRSVSVLGVSPSREWVSEAFARVAGGAAELSGSELVAMLRLSHAQRLPLPHVTLDAVCSQYARILPLMRAVDLCRLLECWVADRYIPSREQLEGFFDAVCDAFGIDLAALKAAAAGAGPDNSAARRSQEMPEASFDTDSASLPSTRGGRDAKGKRNQSAASYMELEMLQAAGGAVSAGAADGVAGSFSIGPAAPQSMSTVAGTASASPAPGNLEPLTLSTAALATAAAQQAPPPPLSSSSPSGAAPPPPSAAVTTAALAVTTAAAAATPADADIVGARASGHIKYTIPSLGSIDGRPKGAPLQPSEAESTLLLLEQYRALLLEFKRSDLLVVMNTYRNGVASALHDMRKTKDAPRKPNSSRLLQAVIRGGDVSGDDGAWSGRSNDIVHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S906</accession>
    <name>A0ABQ5S906_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="592" mass="63813" checksum="9EBAB4F70AD911B4" modified="2025-10-08" version="1">MGNSCNKVTPIVCTTRMTCTVPLWCVPSQAESKVHPAVVEPTRKEDLQKSAQPAFQETTSTPDLPPAPLAVPRPATPPATPNGTAKPAPTLDPVEPFQTGDLVSPVVVTQRLESVAAIEAEPANNDVATSSAAAAAPEITREPELIEVTTVEPDEEVEDLKGVAADEPVEVAPVDVAPVDVKDDEGEKTSPCVYKEEDTNEPVQNAAERGSSKETDSETDDDERSSVSASSKSSTDLGDLRGDEDEGSESRGEEEENEDADASSEGASEAPEPFKFDPDALDALERFKLRDNAEILVELLPGQAVVEALQSICIFATDAGAATHNCQVLITQVQIIVHVLARGHQDGARKAIRSLLEKLHPILVSIQAVVSAWRHKGTMEKFMSGRAADDEFLELHLLLKEFAQSPSFAQLFAQLPPEETAALADFQTTQHLSGYCSVEASRVAFERLLLLFILRYKPTKTRTQKQRLLDLLAAYNHLPSLSNGNGGAHDGADDDGTTQEAVDGFGCKTVEEQIEHLAKTRLGRAATHAVPLMVVMMTNMWGASMENLPTSKTGKWRMAVSLALEPLRAATGVFQLPQVPEDDVRPWVEEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9C5</accession>
    <name>A0ABQ5S9C5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Pyruvate kinase</fullName>
            <ecNumber evidence="4 14">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">VaNZ11_010392</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="4 14"/>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="16">
        <location>
            <begin position="28"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="17">
        <location>
            <begin position="526"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="408"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="675"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="442"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GLI66522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="731" mass="78786" checksum="7354F74F4756E6C1" modified="2025-10-08" version="1">MKAKTHFFKNLDLTSILVPEDGATFTGTKVVCTVGPSCQDVDMMCDMLNAGVVGCRVDLTWGPLDFHRKSLHNLQLAMRKSRRLCCTMVDTLGRELMIRRQVKIGEDGWPIHEESFTVSAGQQVIITTRMDVEASGSVLPITYSKFTEMAVKGDTVYIGRYLVCGADSASLYLEVVDVQGDDVVCVAKNDAVLDGLLTVFHAERSSEGLSNVQNDLPLLSDYDKECLHILAQDFEIDFLSLSYTRSADDVREARRFLDSIGMSNTKILAKLESRQSLLNFQGILNEADGIIISRGNLGLDCVPEKMALVQKTLVQACNLVGKPVLMTRVVDTMINTPRPTRAEATDVANAVLDGVDGILLGAETLRGRYPCETVTTICSISRAAEKVFDHHYHYEHLMEVAIDVGEIGGDHHGAPADSDEDGAITPSAGLAQNEVNGHGANSPSRGPSFRGSGMRGHGHGLRRDDSWNSVTSAGAHHQLPSLGVAAKAMARFSQVASTGSLAGLGYGRQDMTGAAYHRAPYISKLESIASSAVRAADRVGASLIVVYTHTGKTAQLVAKYRPNMPILTLVVPHLVSDQLKWKLEGRSSARQCLISRSLLPVLAAPSPSGDQLLHEAVVMASRVGLVKPHDHVVCVQRIHDDFCVKIISVDELGAGIKRDDAVMSHSVFGINPTHGATGPPAVQHGPGYDSPRVHNNPIGNKFGHMPVPVHLQPPNFTGSLVTVLTPTGLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9C9</accession>
    <name>A0ABQ5S9C9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009836">
        <property type="entry name" value="GRDP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34365">
        <property type="entry name" value="ENOLASE (DUF1399)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34365:SF7">
        <property type="entry name" value="GLYCINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07173">
        <property type="entry name" value="GRDP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="616"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="771"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="813"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="661"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="776"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="792"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="813"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="833"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="855"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1036" mass="114297" checksum="6749C1E2E3E70CD3" modified="2025-10-08" version="1">MAATGPLEDYHLEPFEKAFQKVKAQVSTDLATCATMLQRLLMAVDLFPHGGLYVGHYAEQAIRRYWLLWMPLLHGQQQNSMPVANSLVPPLDVQWAWYVHRLNPVQYLRDCTARFGAGVHPGSLQQALGFSNGSSSQTHKSTSQLQRPHAPTAEQSRNMAQQVWEAAYPAGPGCPSENVFWPPAPSPPPAVGKRQKQCARPHLCQQPHNCRHLQQSQRQQQLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQPEGHENRRAAQSAGKVLRGGAGEDEKDGAGGGGEGDGEGRGGADGSGGDGLPPPPSSPPPPSSPPPPSSPSSSPLPKAGTLESIRSYVVDAMIRQGRFAHQMLRCFYQDPDFLEAGRIRYLRFLALIRTQPPGAPILNPMYDQDVMWHSHLALSGAYVADCEAIMPVGRRPLGHDDSLSAASLVDSFVFTMGRYEAATGLMVNPSPTRRLPTFVAQPLAAQLWPLAAALADPRVTKLPPPRELAAELRMGGMQEQELRNRSDRRHKERKSSGRSCNDRDSNSSSNVQGKQSVIGISGKGAAGSPASRRGVFAGDKDPNPAGGGNRPPMLGSPDHLRRNATFAIYALWNLSERLRLLSAASSQRQVEEEEGGEGEGEGGGRRVQRPQVGGEEDKALAHQGKEEQSQQEQQQKQKQQPNGVLIAALIQNSSWGRQWGFFAACFSVPSSFASSPSQRFLRGDAAAPAATAAVRGDRVGEDISNQELDSAIRHMTNQLLRLGDLSDPCSPLGSHQHPFWDHLTPLPPPMSKPRLPPSQPQHTLNQEQQDRQQLPVLSNASTPTYSVSASSSNFTTCEPRSTTTTATAIAAPGGTGGSADTACRRRRRRRRHPAPAPYIDLPSTFLPLNERTAEWVYLARNQYAVRGQACYTPSDFCLLGDGRWCLPGPLAVEIAEHEAAEEDGMYDDEQADMNGPDETAHRGGYYRYVRRQPWWRIKVRKFFDSDDSNDEGFVNCGDAHLPLYRNTLGFIVFSSRWPDYMARCYHRIYKELHRRRPDRSAVAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9U9</accession>
    <name>A0ABQ5S9U9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009988</name>
        <name evidence="3" type="ORF">VaNZ11_010010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000029">
        <property type="protein sequence ID" value="GLI66252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000029">
        <property type="protein sequence ID" value="GLI66263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="14028" checksum="E7939CFB482283C7" modified="2025-10-08" version="1" fragment="single">MQHNSNPTKVTRTQRFVPAAHVTCPQPAGPFWTPETTGDRAASRSWPQSTEVVPANLLANKMSFTNVKTWVIRAREDNCSRDKVTTNNHMSVSSGGGNFGLDLGATPLRDGFRQVVDDTKEKLMTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAA1</accession>
    <name>A0ABQ5SAA1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin thioesterase OTU</fullName>
            <ecNumber evidence="9">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_010845</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="9"/>
    <dbReference type="EMBL" id="BSDZ01000044">
        <property type="protein sequence ID" value="GLI66858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22793">
        <property type="entry name" value="OTU_plant_OTU1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048857">
        <property type="entry name" value="OTU1_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057766">
        <property type="entry name" value="Znf-C2H2_OTU1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312">
        <property type="entry name" value="HIV-INDUCED PROTEIN-7-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312:SF0">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21403">
        <property type="entry name" value="OTU1_UBXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24560">
        <property type="entry name" value="zf-C2H2_OTU1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="OTU" evidence="11">
        <location>
            <begin position="208"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="79"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="154"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI66858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="409" mass="42802" checksum="1DAE00867954674F" modified="2025-10-08" version="1">MSLTLRCRGPSGQCTLSGVDPGMSTQAFLDLLSEKTGVSSGTIEILTGFPPKAIQIPTDGTVFSLSLANGDTLTVRQSSTATATAAAPAPQAPPSGSAVGALSSSGAQLQDVPQGSATSTAATMHGDPLPYMDEDEMLARAIAASLEGAEAAAGLVDGSAGRSSQQRQQQQRGAEDEGVSGRAVSNAPAGGATAPQSALVPGGEGSCVVRRVIDSDNSCLFNAVGYVMDRSRTRADDLRKVVAQVVANDPFTFNDGFLGKNVNEYCSWIQQKDKWGGAIELFILSQHYGREIAAFDIQTKRCDVYGQDKGYEERALLMYDGLHYDAMAVAAFEGAPEELDVTMFRPGGRDGGAIMAAAEKLVAATHAARQFTDRANFGLRCGVCQMGLKGEREAVEHAKATGHSNFAEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAU6</accession>
    <name>A0ABQ5SAU6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="270" mass="28248" checksum="47FE36C8A3460F55" modified="2025-10-08" version="1" fragment="single">MLKICAAMSAATQSNHPQRCDESALKNSTTVGATSNTGKPGAVKTSAGLQISSDAASSPAPLAITPLTVPLPVATTCSSKACGGAVATTGATAAIPVSAFTITSTPKSGKGSKNKARAQIVAFATTAPHDDMLDGAETDFWREDESGAPLATAVSGTTRSHPRTIYHDAGGAAAATGEGPDLGGAAGGARKQVKKPSKLARLRRKVMSMQQLWIWPFTHYKPTNQVMLCYRTLFQDLGLPGANEDYLAAKFRDELMPLRGWLHRNMPKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB25</accession>
    <name>A0ABQ5SB25_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000074">
        <property type="protein sequence ID" value="GLI66974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="575" mass="61473" checksum="9B53B2730499F269" modified="2025-10-08" version="1" fragment="single">AAAEALTAMCRRHAENCDIVIATFRSSFLWFRRCLLDYMLTLMCARQNDLVIAAMGLLCELFAEKTSELLHQLMQSTHIIGAFVKMVQGWGKQARNTTPSGAANCELARGMLGLLNTMATAGGTKVCRSLDKGGVTALCLQLVEECPQQQSLVRGAMALLSVMVLQVPAQRLEFEKNQGVQRLLQLLNQGMLDLSVRGMAAEVLSSAASSSPKVADTIRLQGLATVTDLLASYLHEWLVQGVEDPCSESAEMESSVPTPQLPPCNTEILEPLLRLLATVTTSSTGEVPLSDQGLKLLGVAVRAGLAHTAGEGYKVATAAAQVALHLSCKTDVPLSPALCGDDVTEPMIEMVDLACRRAAAGDVKDARILHGGLCAAGFLSKVLGARLRSNDVQLAEVLNSDLLIKLVTLLGRSADAVLVAVNAMEPCYTEPITPEGLGSGLRSTGLTYLVTRKVVADLRAAVPLLLQLLINLASGPLEGLQVLVRLGAAAAVKQLMRWEDMKAKAAHFLRLLDELDLEYKPFGDWGNGPLTAAALSALQLDPKPFVAQAISSSKLLKLRDHDLRTRFGLDQIGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBJ4</accession>
    <name>A0ABQ5SBJ4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="606" mass="63443" checksum="42A57F84535A9F3E" modified="2025-10-08" version="1">MILPPIRSCITWGLGPANLDTVFNSLELEVRSSVAEVKGLDQGILSLEGELELALRQREHALNALASMSLARSANSVSDSDVHIRKNSPAIDVVREPVVSMEPQLQMDEQHADQGQPMPWQNGSSHAVQRSEDIEAAPQYPVLTGDPKYVPARGLRPTTAPCSNTEEQNPSPTLNADDVKKRLLQVLRHLRDTAAFCREIPDLDRGFSNMLKKTYKELYDSLKELTSKSSGSRPANDATAPRPAPGGASTARQATAQPDAETQAPNSTQRAPGPVAVGSASTGAFAQPTSSRDLSALARLIHAKRLHQQADGAAARDPDQAPVRRIARATCASRNSRQPTLYDGLPPTRVAVGPSQGAPTGPSALQPATSEPAADADPNSQTQPQAVLGVDAADASMPETARMPSPTRKCMNSEGGETVFRQTAARESGAGLSTTCRVTESLAGSNGGVNNISSPARQMLQHARTSGAGLALILLSKTAHPLGPVVPHSEGRAGLFQEGAQGHPATQAPAIPNGASNACVVGATGGAPVEDGYYRVVAHGVGAETQTCGRVVARADNSTSSSVNGRGLLAGKRIKRTIGMDKNASLKSVSPGRKRQHVGDAQLPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBJ7</accession>
    <name>A0ABQ5SBJ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010831</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000044">
        <property type="protein sequence ID" value="GLI66848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF110">
        <property type="entry name" value="INACTIVE ATP-DEPENDENT ZINC METALLOPROTEASE FTSHI 3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="361"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="445"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="700" mass="74531" checksum="09A7EC27128CAA73" modified="2025-10-08" version="1">MSAVFQKQGRQYKCMANRRHHEKLSNKITKMSTVSSASLLGLGLPRLVNERQMTPSVLCPTARLSAGGIRPRMISYVPKLGNGLVFKPCMHAVFQRSRFRNRGRLRQSHQTTATSSGAHQPQQRQKNPSDQGDGPSTSTTDLFDSQQPQTPWLVQLLQKLKAPRWLIQFVHKYIVPQPTARTAALARVLWIAVGFMTVALGRELFFPSPRMTPREVLYSDFVTLLDAGRVRAARLEAGTSRLYFDVTRPQPPPSVVLPQQGSVSSEVATAAAMAATTTSPPATALAAASSAASATDSGTGASAATQTSAPSAAAASASASVRQRFLKQFYIKLADKQDILLMGKLLQAGVEFSVLRASFTAAAASAFLTALALWLPLVPVFWFLRRVIDQRQGAGRAKKASNTGNTPSTTFADVAGVDAAKAELLEVVAVMRQTKGSYSKLKVKMPSGVLLCGPPGTGKTLLVAGEAGVPFFAVSASEFVELFVGRGAARIRELFAEARKSSPCVVFIDELDGVGSRRGMGYNDERDQTLNQLLTELDGFDGRPGVLFLAATNRIDVLDPALLRPGRISRKVVVPLPDTAGRAQILAVHLRGIPLEAGLEPATACAALAAVTEGFSGAELANVVNEASMLAARDSREEVGLRDLQAGAQRTRYGVNGRSGGGAFGGGLWTERLQNWVVDSVASAAAAAERRPVKVGTGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBT9</accession>
    <name>A0ABQ5SBT9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_011106</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF194">
        <property type="entry name" value="INVERSIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="507"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="540"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="573"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="651"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI67010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="75824" checksum="78EAAA4A0C50C3CA" modified="2025-10-08" version="1">MRLLNPYAPEWTPAAMRARAGIELKAEVDKLNDTTFDLAGLPSEVITLVVSFLTNPRDLISCICACELLKGHVHDAELVLDIRSWRTSSGLGNGGNIEYMFDNNPKALLGLSKYMPGTVALLLPNLSLEDNDLNYLRLMLPRLQMLDVSGARKITHKGVSALTRPTTGALSLSIEPSTPASSPTPDNGLNFTATASQKPDATAVLISTTPRSLLPPLRFVNLQRCFQLHSGSLDELLAAPGLRGLALSHLDLGRWPSGSGSGSDASTRPGLPTRTADLQVLVLNNCLRLHGGAIDALAAACGGEQCGWGRGGYTSTSSLRFLFLGGSTLALSAFVNSSCAPPDLPPHLSDMLASAYKPALTSTATTTQAHLAAARHLLAAVLSLSRLVALELTFFPQPLVQCLRRVLSDREALGARKPVQIWDFAAADGTDVDVARHTLCRVLHLSRLGVQGVVEADGTIGSCSWTNGAGLGRPLAGVIVSGRTGEALERCSVATVLRCAINCSSVTRSTPLHLAAERGNAGVVTQLLAAGSVVDARDTAGSTALFLASETGHTAAVERLLAAGADPSLGNAAGETPLYIAALRGHLGCVKELLVYCNARGIAWQDPEMYGDAWTPLHAAAVANRADVAAFLLQAAGSAGAGELVLTPNKYGQSVLHVAARKGSSQLLRLLLSAGGVAVVGSRDGSGDTPVDVAKKNRHVAALAEFRRLTGGHTATRGSHTAATAMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC45</accession>
    <name>A0ABQ5SC45_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011418</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="517" mass="55832" checksum="6B28F52755E4FD4B" modified="2025-10-08" version="1">MFCGDLTVGSAVYRLCVAFGRFAVVTNAQLKLPTVKGLSGGWQIDFTGNISGARFIHPINTDKDGSLVAARVASALSGLSVAATSDVRALSINPTRMADAAYVEDAANYVVNQLYGTLAQAGTNPLLQKTTLVLSSVHRYGLYVAEALHARVLPLQYISFANSWQQVVNASSSATIIVGQDYDYGGLWLWNKIAAGKAATKAANVPTAYLQAMKDATNIIVVQPCDNWVYCNPVSDPQGYNCWDAINETYIGGTSGPIYLHTSLSRSGFGTGGQLYQEARAKGAIGKASAAEVKNLKQWEWGVPDSTVNNLKTIWTQTLKRSETNFKVISGGVVDMFTWTPDVWRSYLESANKLKVRGFHWNSYWVAHTQLERMAAVLPFPSYSYYQPYWHPLDDKARSVLNTMPGAPNFAASYAANSRAFVNSIGSAYDADGVKRVLSDYGLTPGSGLAYYGFGIDAAGASSVGWDGTTNVRAGYVEAAVALQNATLAPYTKRVWMPLVFANFTSAPSVCGAWTPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC65</accession>
    <name>A0ABQ5SC65_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="785"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="785"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="893"/>
            <end position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1503" mass="159374" checksum="2F123F585A11569F" modified="2025-10-08" version="1">MFENIAGRIQLLCGAVARLQNQHRKSARYGHFLSSSKILQLLMISCLISWSVLCCGSFTTRKMAHSNDVGQSVDREHLYSSGFGDNDENNPNHQLHSNHFLDRHSDQVRHYSLELPESFMSAVGTAAAPTDNVPDQTEIRDLPVSGTGSGIEHCNGNGNCNGHVNRNLNDKGEDDWEVFEGIKSLSESFWAGNGAKRSNSKTPNEDVSAPVVQHPNAAVRLLHVKSDLENKPMTEDEANAILERILMFRSMQEPPVYPPPSPSPPLPPPPPPPPPPPPPSPSPPRPPSPSPPPSPPSPGRPSPPDPPSPSPPPSPLPPDPPPPPPSPSPPPSPLPPDPPPLPPSPSPPDPPSPSPPGPRSPSPPDPPSPSPPPSPLPPDPPPPPPSPSPPDPPSPSPPPSPLPPDPPPPPPSPPPPDPPSPSPPGPPSPSPPPSPPPPDPPSPSPPPSPPPPDPPSPPPPPSPSPPGPTSPSPPPPPPPEPRIPALSPPPPSPPPPSPQPPLPAPPSPLAPPSPQPSPLPPAPSPRPPTPSLPSAPRPPSLPPSPFPWPPVPSAPPVPSPPVPPSPRRPPRLPRSSPPLEPSPPTPSPSPPPSPPFPPPPAKQITANNSLVSYGSTYCTPTSQFDQWYDTLTEASPGFVFDKILSLTLYSFDSSETGYGISIVAMKVKLSSNLRKQNFTIQLSGSMYRAGYYNSESFDLSQRTLTSLKVCCNPAQQFGGLGFTFDDGAVAESQGCLAATQAPPSQQLKYYQAPVLPGNWLAGLRVSAGVFLGALSYGIGGSIPLPPPSPPPSPPPLNPSPRPPRPPPRSPPLPPSPMSPPPPPPGADLPPPPVPPSPSPPVPSPPRPPRPPPIPPRPPSPPPPPSPSPPSPSPSPPWPPSPPSPPISTTLKPPEAPPPARPPPPPPPPPPPPMTFEEVRDRVREIIQNEAPNIIANVTQPDNPLNNAYAASINVLWGVSTAIAVGETGQPVISTAKYGRGRMGIFGAQRMVSECCKPIFKGKPTLTKRPALDLLIQNIAIWTVSPEGSQPSNNTSTVCVSSKLFQPLARFLVQRGGGKFRTAKQAGLRSLVVPLPSFVNISTKLAQKCDLYVISSYNQEYEDESTQDFMVSYVARGKGLMVAGPDVMPSIFYAPNAPAVPPRAVTRRLGQKDDEPVASTASTASSSNWTWWPTVRQFEHGNGGSGADFERRNLQISPVNNKDLPANKFLLRMQVLFSDAISDSGGAMKLVGFGTTEYVTNAVLAAQNYIDALLGNSTLSSPALYDAVRTVKQANTTLRESKYQDVPGTELILQFLDGIAELEKAGYTLPSFELDPPPPSPRRILPSPPPIPWPPPPPLMVLPRNAQFLGCFADYSPVNSSINGSFPDIVWIPKSNNFTVDRCLNFARNQSLYNQAQGYYYLALQIVSYPGIRRCLGAKDKPTDFLKRYMISNSSCDTLCGGSFNQMCGGNPLSPSGTPTAVYMTFPDKNMTTAGGPLSTASTWNLNFKNMDFDLADDDDSNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD57</accession>
    <name>A0ABQ5SD57_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cilia- and flagella-associated protein 43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_012130</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CFAP43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14885:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14885">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 43-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25828">
        <property type="entry name" value="CC_Cfap43"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="541"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="793"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="966"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1262"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1466"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1695"/>
            <end position="1721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1782"/>
            <end position="1803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1833"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1967"/>
            <end position="2031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2387"/>
            <end position="2442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2461"/>
            <end position="2489"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1530"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="796"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="981"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1265"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1310"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1695"/>
            <end position="1706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1707"/>
            <end position="1717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1851"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1967"/>
            <end position="1983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1992"/>
            <end position="2004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="2012"/>
            <end position="2022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="2476"/>
            <end position="2489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI67840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2489" mass="261451" checksum="BC5BF6C24CAE3B12" modified="2025-10-08" version="1">MDVLAITSAGYGGRVCFLDETNLCYASGHGVVIHDKETGMQKHIWSLPDGQGNPPPNMCPATFATCPGGHLLAYVQQGGQQQQIQVVQQETLLPASTIKDPGTRHMEFLQLVFDAEGTRLASLGGAPDNQLDVWDVDLKESLLRVQLPDALTGLEPVFFPLNSEVLAVGGPDAVRLLWDEPLGGARIVRQSDVDLAVLAEGEQLNCFAWTPNGTLLLGTTAGRVLGIKGAAPPAIPTGSWGENPGGGHAAELLYGPAVPTVPPIPPPPAGSPPPTSLLIGPAPVRIGVVVIVVTATHILLVMGGDATSSAELQWLSPQSMNPIATAPLPLRALVRGAVSPSYEQLAIAAADGSISTCTTVCPIPGILLGCLTGDDGSSASLAAAAAAAGTVPTSSNGGDAPPAMVAEQWSASASASGDPGRGRGSTGVGGVDGAAVAAAATAAAAAASVGTVERGALVQVTEVVRFHRGRISACLWLTPTRAVTGGMDGTLRMWSYVPNDGDPPERAGWLSPGLQLDAQWNVSQPVTCMAALNPPLPVGAEGPGAAGAGSGGATEGGGGGGHTDFATQWAASAPKPPAFMVGTAGGVVQLVNADRVPANMADVQDLAQLNASLRSTWDVQVFGAPITAAAFSQDGRFCAVSCASESKVAFLRVMVLGHELSMRVLGFYPMREPRHLAWAPPEPGGIHPLLVVSTILGEIVVLGLPDSSALEDGPPDGLLPRGSLLKASMRAQSHVTSLVVYPLPASEARGGGHFWVITTHTDKSIRRYRMFTDQQKAAAAVSARAAALAAAAASSGDGGGGGSGGGGGGGGSSVGGTTFRQINPSGGNARGNSAVGANLGRAVSMAPGSQNFQMPEAEKPMELQLAANELSISMLPGGNAGGGGGGGGGGYGSPLRMAVASSDGSVAVYSMPEIALQGRWLLHEMVCGGATGVCLLGDRLLAAGGEGTIQILEIISVAARARNRASHVGAPPGAGGSALVAPSPTGGTAPSPTGGTAAGRVPGQVLSTLAQRSAFFQPAGPPRPRGMERATLGSILPVHLQTVLPVMYTDADAAPTWQEARAQATRELYREQLTSFRKKAHAKCREWVERVKELHKINEAADEVERLTPEEFMIDVGLRNTLLGEAETRYARVKAQLVREMRVKEFQRQRLKEECMSVMECVSRPLVALGEYTFISPMDPAEARRYRFEGEASAARTDGGVWSYGLCKMKAAQELRLKQVSFLRKVEQAEHALRNSMERGAVVPIRRYKGDMQRKFTLLSEEEQQATAAQQQQGQQGQPLGQPLQSGQLPVATGQGPGGLGHPQRVQPTYDDDVEEDETQSVGGSSMKRTRPIRDDGFEGTESGYGETGLMYADVQLYTHCRRVSQLVFIREEIRCLRRLFNSKWEASRAAKQKSLDRIDERLGRIVEIQKELTKLALEEGAGEPPPPAPQHVPGEFYMWTPEEEMVALTAVRDEEVHAAKYISPEERARRAAEAAAEEEARRRAEADDARNRALKEMMNNNPAISRKSKGGKNRQVREAWMDLAPGQMTKEQRLKLAEWERKQQELAEELDKHRRLLEAERRALEVDIAEIAAGINTDLTRLSNAKIRMDMEIVYLERRAADLADLMASLSAARAARSALTVQLAANTTRRTRRSPLLAALEAKVQAAQRAYEDVALSEKQVDKAFRREFSSRPELWDDAQKVYRARAHLLQASPSTGSLGPAAGAAGGGGGGGGPVTTARLPRKSALELLGKSPEGQALAAVLEVPGPGDVSLSQSTTSVAPVSRQTTLSRALTGRLTTLDSLGGSSAAGAPNPDVLLTTPRRPLRDMAHVARPLPRPGSALQAFLQKHLEPQPKGSDGAAASGGGSTGNAPPTQPLAPATPRHGIGAVLIMAGEEAALARRGVSQRPAGLRLRALVEKEGRPLDRLLQMVADGLPPSALTVELGPLGLRLGSNMPGNLNPYKLEGPASIFNPEVTGAAAALSDVGGGNSSAGGSQAGGGNSSPVRERSSASGGRSTNNGSISPLKPDEGAEAEEEEEDPLDSKERPEGMDDSLWIRLLEFRQTRADLEMEAEEAWRRVLLLTAQLDWIEAEDASMEAAVEEAGARAAALRDGSTRLSLDVSLTYRLRNGQVELPERPSDVVGAGDVLADALILHRNRVESLNEEVLSRGESKIEVLEELRLMRRKIHLTQWETEAAELAGQHAAQRLTELQLLHVTKDLQAALRDPTIVDRLHEEDRLLSVRQNIANLHAHKLEQRRRQLGSLRARVTRSGTEQGELSVAEAAAEAAFQESTRMHLMMSHAKASEDASHRRHMRAIVTNAQLRAIAQQQAADLEQFSEQLRTAHARNFPALKPMLAWAPPDYRPASAAAAAAAAAAAIQPPGSRAASRGGDPSMAMATIASRPGSAAMPLPGVQRRASSGLQTRSSSGGGPGSRPGSRAGTPPVPPRSGPLSGLTRSGGAGATLTRAYVLATGGGAMGAASATAMGSGGVNGSSASRPQSSASRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDK2</accession>
    <name>A0ABQ5SDK2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011732</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046853762" evidence="2">
        <location>
            <begin position="37"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="484" mass="51853" checksum="45A0A650A2BD2536" modified="2025-10-08" version="1">MFRTNLRGRGTPTSVKVTTLALAFILLGIYIHPVLADDGERGSHITETGSAIAAGLSDNGHNAYYPSDGDHDSYGPGGGDDSYGPGGESKSRGRTEKDKLPFRTGSIVRLVSPWLDYACDTATDPAPYGPYGPGGDDSYSPDGDHGSRDDSWWIQRRMLRDVNSDGSMGRKVSLGGNGKGKSYPYGPDDDDDLAKGVVWCNVTITDNPPQPYLSEARSKDQAGNGNGGFVSSWLDPILSAFASAGAAAFSRATAKHMDDVAQLSGSGSSGGAVKGSQWDLDGFVLMGTDFRTPYVFSGGRVFLKNQNSRKFCRLADDDDDDGDFGLPSATVVCDEMTSSAKTEFVVEMVDEVKEDEEAGRSHVMDSSAAQRDAKLEGYGPSDGDWPSGRDGGKGDRPDGGGPDSGNDLWPGRGGKNGKKTSVMIRLRAPQNDMRSKELWRRAALPGRHWMRRGWIGCDGLDDPEEYGLRSQSTAFRAVEGRHMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEG2</accession>
    <name>A0ABQ5SEG2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012674</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Sodium/anion cotransporter (TC 2.A.1.14) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17380">
        <property type="entry name" value="MFS_SLC17A9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044777">
        <property type="entry name" value="SLC17A9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF446">
        <property type="entry name" value="SODIUM-DEPENDENT PHOSPHATE TRANSPORT PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="503"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="533"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="152"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI68313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="574" mass="60309" checksum="362B5A94204BB345" modified="2025-10-08" version="1">MMASHCWRLVRQPCSVPRQNGVPFTCPAALLRRFRAGNFFSGIDSANLRRGSCVSCALSSPNRGDGLPEDGIPRIIISTGANDKPSTQASRRRASSSPRNLSSATEALAGQRSAANMESPPLLARSPTYTTREALRGPHEPGPPSGFKWEYLVALCTLGVVICYADRSNMSTAVLPMAQQFGWDKAYQGVVLSVFFGGYATTQILGGKLADQYGGKMVLAAGVALWSLFTYATPAAAAAGTLPLLLSRVMLGVGEGVAFPSIHSLIARNVPVHNQTTAVGIVTAASYAGTALAFGISPLIISNFGWEWVFYSFAGLALLWLPLWLPIQTVDKFQPQVAAPPAAAGDVAAEVAVSGDGSRPWALLRRREVWAICVAQYAQSWGMYGLLNWLPTFFSDFYHIQLSDLGSYTLLPYVFQGGLGAATGFLADYLIRSGWRVGTVRKILQVAGMLGPAACLCLAVSPLVGASASLASGLITLGLGFSALTLGGVSASHLDVAPRNAGLVFGAGNTAATLAGLVSVPVTGYLLQTTGSWPLVFGITAFHYVAGALVWSLWSGDRVLPEDGGGQRSEAVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEP2</accession>
    <name>A0ABQ5SEP2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UVR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34957">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2 (NTF2) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34957:SF1">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2 (NTF2) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13474">
        <property type="entry name" value="SnoaL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UVR" evidence="1">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="SnoaL-like" evidence="2">
        <location>
            <begin position="87"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23361" checksum="314DADDDE67248D2" modified="2025-10-08" version="1">MQVRSSSLAAVTRAQGLPGQCGARLTMHCRHHIPAARWLCRAALDSDIPIAQLETELQGALTQEDYKLAARLRDKIQQKQQVSKLAVEDANRRFYDAFMSGRIEEMERVVGEGEHVQVVHPGASCIAGRAQVMDSWRAILRNVRPGAFKVKLEDVRVFAREDMGFVTCVEIIDADDSEGRIVATNVFEKQGGAWRIVQHHGSPTIARFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEY4</accession>
    <name>A0ABQ5SEY4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012577</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044589">
        <property type="entry name" value="GATA26/27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46855:SF1">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46855">
        <property type="entry name" value="OSJNBB0038F03.10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="3">
        <location>
            <begin position="3"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="163"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="206" mass="22415" checksum="A9FF3C693D09C99C" modified="2025-10-08" version="1">MRGSNGGPCCHCGTSQTPCWRRGPPEKPTLCNACGSRYLVKGSLAGYFPGARRSAVNSTRSEVVSHRVVVTKGSRKAALPAAAGVKRKSHELNGFKDQAQRIYNEYEALEALRYAYFCRGPSFIGHGDGLGATVSDSSAEAHNDEDCFQKEAHYLEAFSDEYQEQGQETPIHHPLDATTGTGVSGPQPAMFHRRPRKQLRPVACSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFY0</accession>
    <name>A0ABQ5SFY0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="456" mass="48010" checksum="9BED4620E76BC41F" modified="2025-10-08" version="1">MARQRRLIQIARRMMPLTGKGHALSTDWEKRREQANKLIYRKHGIPNAVQLGHTLATHTSLSGKDRVRPGSQMTWPGLPANSPAGVCRGGIIEVCSPGQLCRDVAAQMALAEARTGSVLWMAVGPQAVGFSNAVLREVPNMSLQQRVISIAADSKSALQELLRLVRQQTYTMVVLDHVAGLAPPPTSWAIPPDPAAHPQALPAVPAAAAARGAAAATTTTKTRTGSEATGTRSRVVAAEDLVGKLGAAVLEGTAAPAALMQAHHPHPHYHHHYHHHYHYQHDHLYQGSAGVPTDAGFCHPAIPSPGGGSAVLNLKFLLNVLPLLIEETLGELAASCGPDLRTTTLLLNSYAWRERARAASAAGLGADGGGRSRWARRAILRHAAATLHVTPESGGETTAAVPGAVGPAGAPGGQGPVRCLRAVLSKSLPTGEDGDRLCTWSLNTPIRLVSPPWDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFZ9</accession>
    <name>A0ABQ5SFZ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012628</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="58"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="363"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="413" mass="44767" checksum="DCBCB27FB2050270" modified="2025-10-08" version="1">MLPQRSITTLLHTCTLSCCAHKRKAIFNCIDFQEASFINMSQQHLNDSQSYGGFGQPLASVFVVPHPTRPNEDTFCICPLWFGGLGLVSFFGVYDGHSGQVVSRFLAASLHHYVEAYGMIATDVASALCQAFQAADAALVEATLNCPDAGSTATVALLSANNIIAAHCGDSRALICRGGHVMALTEDHRPARLDERERVLKSGGQILWNEGERVMGILQTTRAFGDRDLKHFGVIAEPEAVICSRTPEDEFLILATDGVFNVLSNEEVADVARRVLRRAMERGASRSAAIQMAASAIGRFSRDRNSKDDITVILVDLAPLGPPCPPTSPQPAPMAELLRSKTALVPHYQQQHVLMQPEQGIEQQAEQADSQQQQQQHTHHRHQPMCASEITFGAPAAAVAGPGPQFQGRLLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG04</accession>
    <name>A0ABQ5SG04_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013199</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="212"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="358"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="400"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1648"/>
            <end position="1680"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1681"/>
            <end position="1717"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="1718"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="1886"/>
            <end position="2184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="474"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="642"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="799"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="969"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1188"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1249"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1776"/>
            <end position="1811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1825"/>
            <end position="1866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="179"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="653"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1188"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1779"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1795"/>
            <end position="1811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2223" mass="227336" checksum="456930E535049F90" modified="2025-10-08" version="1">MPEVEPDALVAAVLHHQPLQKLLRAGADPTVRDQAGLTPLHHAVLAGNVDAVGALLDHACKRTRPLRRAGGDPSRLPGSPWDLATAPGPLPSRASPLHLAAERSDSLLRLILGHNSLNCVAASFLGIPISTRPQSELLHLRDARGWSLLHYAVSRANLSTMRQLLPHMGANATELGTNGSSRSSSAGGAAPPHAAHHAPQHATYGVNVPDAFGRTALHQAAVLGHADCCRLLLKAGADQSLTSAPPEAALPLHLAASGNHTSVIEVLFRHAVNQNGFRAAQSVLLRPNAAGLTALHVAVQHGAVDAARRLLLLVFPFDTRGPLGRTALHLAALLGDTPMINMLLGHSTPQQHQLRDAEGFTAMHLAAATGRLESLRVLAAGGCGVDVRAEAAPHAPSGCEGWTPLHCAVRRGDVAVIELLVSDLGADPHAKDAGDRTPYDLAVAVAGDASPSGSSVVGSGSGIHNLDDAHWATDATATESEEGSRERAASSTARGGSSAAATAMVVDPLQGPSGLTAGMLGFNGPVGSGTTRLDEAQALAVLQTFIRLLHEPVQRSKALPPSLISTLGGGISTQSPGGGVSLPAAATSALGNPPRMMFAGVTCESSGGGSPMKRLTVTGLDAAAVPPGSSEALRVMPSGLATAPELPVDRGARPAPRQAPPEGRGTPDGQAHPPVGGFPGNNNRPAAAAAPAILVAPEAISAAAATGNAAAVLGREWSPVTAPLVNGVAGKAALQANIRPPGYGAAAPVAKVAKADGCAHCQDSALLPSLDPREPPHVGNGAEEASLMAVTYGAQMLTGPSTTPARASPFAKPPAKRPSPFLKASTAATAPGVPAGATARATSSGANAATIVAPDADRKEQMTVTVVVGEAAAANGDTVGHRVAAPCATANVAPDSASTVRNSVMAAIRIEVGPTGAVSPGPVDVVDPSPPVPPPAAIKRSPFVTSAKMSPFGAAAAARRTPVATAAPQLNPQPQQQQERHPADTSTAASGTSIVMGTAAAAAAVEAEVPATVTVIAPMPAAAVVSPPVVETAATAAAATAAAATAMSGDGMATVASLIEASPPGGDQEQPRWSVAPFSTSAAMQPQNTTFSNGLPNGAPSDLAQSLCVVSAINSPCGYSALNRAILLQRHGLVDALLGTLPPQGHLGGVLVLGGTPAGGGRPAAATVTYNAANMSVTGAALLSVHGGQNLSPSQSQQMPLAPATSSSEAVDNGAGGQRPVQSRALVTRAATNPTALVSASPPVAAAAAAAPTPPAVGPSIRPQTSPRHTPSHMQLQLRQLNCVVCHSPAASDGAGGTVADMSSRRAPPLLPLGGPSVAARSFTASTGTGTALIPGLIQWDETNEVFRTQPPNHTSRAWLHWPGEPDPSTGWLVAELVVVESGSAGGHVWLGLGPPPDVLAAASSTCNGTTNASAGASSAAAGGSAVTGLATKGSSVAASGMTRVATATSGALGPGGAPSGRAAGGGAPPLIPVAMCPVINHRSTHVACLRDGQAFRGGIQAADLGAATRLEARNGDTVALFWNRRGRSVAYTLNGRYCGRLSGLPVNAQLTPLVGLQCAGFVFKWRCCSRVGNGGSGPPEPYTCPTCTSNIDGMLIREQMLHEDPETPWLCKPSMTSLHFAAWSGNVRVLPQLVCAKGFSPEEVDVDGWTPLHFAALAGHDEVVRLLLSLKCPPDKKSKYGSTPAMLAAKYGRSPEHVTIVRILSDVGAVLDLRDPRGRTLLMLAARAGSLAMVQLLLERGLDPSATDALDREAQQYAGQHAAIFRLLRNAQAAQRAGARKGKRRQHGHQHNEQQQQQQQQTAAIAATAAGAGTQTVIAPATDLDGHEEEGESEKDSSATVSGRDSSDDSNGTDVEGGGAGRGRDVDVQHLPILAERWRVGAGQLLRGRLIEQGAFGAVFEGTWCGARVAIKQVMRRRHPGPQSMAGVGNVGSEGEEEPEVVREALSLEREIAILAALPPHERIARMLGSVKLPGDGLCLVMAYYPHTLQSIMQSERLRRSWLTPSRRAAIARQLAEGMAFLHGLPGPRIIHRDLKPNNVLLEAAPSLGVKICDFGLSRILAGNDVQSSSAVGHAFWMAPELLRAQPYDEKVDVYSYGVILYQLAYWVDDQLYGGLNKAQVDFQVVHGILRLQDRLPGGIDPTVQALVRDCVDESPRNRPSMSQVLDRLSSVRELSQPGHGNVPMHSTVVAAAAGAPGEVQARAAGGGTAAFGGDPEHLRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGP3</accession>
    <name>A0ABQ5SGP3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013368</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18092">
        <property type="entry name" value="SpoU-like_TrmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033671">
        <property type="entry name" value="TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43453">
        <property type="entry name" value="RRNA METHYLASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43453:SF1">
        <property type="entry name" value="TRNA_RRNA METHYLTRANSFERASE SPOU TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="7">
        <location>
            <begin position="6"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="287" mass="31166" checksum="B96898F2817150A9" modified="2025-10-08" version="1">MSYSRWDIKYKVSQRTAAGADKWLDVKLWDSTTECLAAVRAGGYQVITTHLSKSSVTIQEVDWTRPTAFILGNEKFGVSEEAVAAADACAIIPMTGMVESFNISVASALIMYEARQQRLRKLGSHADLTEEEKMALKAVMLSKTVVGAICGGGWSAGPLLNHGKVLQVHHLSLYIITAFCVRYRAVGVYRVRVDHIFPSQLGVQSVRCCVHSGVSLHVPPISVARFIARYGISALNPYMQKESKTVISELLSRPPPQWQAAAVKATAKQAALYQAGVAARTANDAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGS5</accession>
    <name>A0ABQ5SGS5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="119" mass="12751" checksum="D990B972DA910432" modified="2025-10-08" version="1">MFSSSIQLRIPWDFSVACSSCDGPSTTQHQYSGRTTRMSLSGAPEHLQQLFTTCTSTFSGPTAHHYSAGNGFGPLLWQRPLYALPHTSTPRSISGTDAVRAAASSSRSRPYLQSVTPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHB6</accession>
    <name>A0ABQ5SHB6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="339" mass="35121" checksum="964E6AA5C6BDC2C6" modified="2025-10-08" version="1">MFSGIARAAARPAAAAAAATAAANAAQGSVQARAPPQGARQAAHAPTHSKIVDRDTEGWVLAELKDEMVLFEPFNAKEVDEAAGTVMALFGGRSSTARVASGPIITEVGDDVDDGLSTASGSVDDGLADAVIEATASATADAVAMGRNDERKQLTDLMAITTRLLRRPGMQREVVMCMFEDPEVRQLMMGRCNDLDRYLTAAGIHNPGLLPPPETITITGDPSSTGSAAGAANAQRHGGPDMISRLVGAVVTVLQRAGGALAPLAGWLRRRLQALFAPENEERMDEGPGAREGAAAGGGGALGQQKTGQVLRTVMVLALAVFSVMIIRKPLVLRALSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHF3</accession>
    <name>A0ABQ5SHF3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GTPase Der</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GTP-binding protein EngA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_013435</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01894">
        <property type="entry name" value="EngA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00195">
        <property type="entry name" value="GTPase_Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016484">
        <property type="entry name" value="GTPase_Der"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032859">
        <property type="entry name" value="KH_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03594">
        <property type="entry name" value="GTPase_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43834">
        <property type="entry name" value="GTPASE DER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43834:SF6">
        <property type="entry name" value="GTPASE DER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14714">
        <property type="entry name" value="KH_dom-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="G" evidence="9">
        <location>
            <begin position="54"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="G" evidence="9">
        <location>
            <begin position="345"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="GTPase Der C-terminal KH-domain-like" evidence="10">
        <location>
            <begin position="534"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="238"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="633"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="673"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="808"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="855"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="936"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="994"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="645"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="673"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="690"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="704"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="718"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="818"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="867"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="956"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI68888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1015" mass="108011" checksum="80EFCAED7DF513A9" modified="2025-10-08" version="1">MHGRTTCVIRLLSQSVGITMLEAPFKQAARGVRRAAGAREHEIQQRVDNDLLPKVALVGRPNVGKSALFNRLLRRRAALVYDTPNSHVTRDYQEGRAQLGDLVFRAADTSGLEPRGGSNRGSINARATVITGALLRQCHLALLVLDAKDGVLPADEEVAKWLASHVPRDQVLVVANKAEGAKAREEMPQTVYDCYRLGFGEPVAVSATTGDGLADLYTALQPHIDALTEQLAAKAEADGNAAAEAASGSGSPSGSPRGTGVVAAVADDGRETSTLPQDGSAEGAESRRVRLSLRGPRRRLLFRRRQGADTATATEAAEDNGSTVAGDGVSIAGCDSGASGGVLKLAIMGLPNAGKSTLLNRLVGEERALTGPEPGLTRDAVRASWMYGNQPVELIDTAGWIGVGRTASYDDVGGEVASMSRQATLRSLAQVHVALLVVDVERALRSNRVLARRELSLASHVIREGKALILVANKMDVLTQQQRALYRKSLREHLSQRFLEAGQLPVVEVSARDGLGLDAIMPVVEEAYQAWNKRIGTARLNNFIAKLSARTAGGGGTEASLRRIRYMTQIKSRPPTFVAFLRGTASRPVSGDFSSFLAHQIRDVLGFPGVPLRIWFRYRDRVAERQHRMVSIRSRRGQLPRGCGLDSGGGSGRGNNDGRGSIMADALRRAEELRREGGDDKNESADNRDVVGVGSDVVGRSIRQGGTASSDTTAISEDGDHGNEVTADKMTGQHGPARYMPPRLRRPRSTAAAAGSELNIRSNAKSHSDGGGQKADGTASDFPIPDGDGSSEVMAAVVRSKAHQAWPKVPVPRGTAVPHSSNNSRGSARTTRLQVLDPYGDEVDGDAARDGWHLDEVPPPGRDVLTVPQSLNQTPSHSSNHPGFRSTRQERGRGEGVAAPSDSEGVQERIPDELPAVMRVADRVGTAVRLDRPIRTGTTASKGTALGVGGGMVTAESGTRTHGSGLDSGSTAADKGSSTRTLTRAELMRMMLFSRKPSAPLVPVQGTRRSRRPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHJ9</accession>
    <name>A0ABQ5SHJ9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013162</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF219">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION TERMINATION FACTOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="101"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="562"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="752"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="899"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="251"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="573"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="757"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="797"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="871"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="905"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="947"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="958" mass="100446" checksum="78E9F9F021CDEA28" modified="2025-10-08" version="1">MLQAGFPCALHGSAKSGWGASASFRRRLLFTISGAIGSVGSPSAVEGRLRGRSSWQRQQSRWDQRVAEKHNIALKHLDKISDRLQKQLLYLDLDGEDGDRDTFARPSSDAAASSSDSNNGSKSYRRTRTGKHGHNMGGQSLRTASYQAVPPSPEVLVALLRRAETKRALLQELNICSPLAVSSRLLLLSSLLEIPAHRLVTMCGKCGRLLLRRPDQLQAALSALTDCLGALMPTQPVMVPAPEARYDDSGSSGGGSGIGAGGDSRHKAVPEEVVRRNRGGTGGTGVEAAAVLARDMAAAAPELLLVPPQRLRRSGMELGAVCKARGIKVLPMLSVRPDLLLQSAASLAAKMDVLPGLLGLPPKRLREVLAARPDLLRRSPRQLSQRYQLLGQLCGVPPDFLAELIAQEPGVLCFGAATLRAKFEALAGQYGRFQDDVADMILVDPSVLIKAPAAGMAAAAAKESDDCKAGGAAAADIPVLDQLLQLSAASGIAIDSVLKLVVQDEKVYGYSQETVAARLSALEEALGLQGEAEARSKVAEHPGLLLLPSEQLSSRIVALSQMLQPPPEGGNSGSSSKAGSGPVTVSSVKTVTTLVQRQPELLLQDPSDVCSRLAQLGQMLLMNTAPAAAPTSASAVTETAAATAHGGIAAEQQTTLPAPPLPPQLLSLVASQPSLLTIPPEETLDKLLVMQSLFEIPRALVLELIFREPALLAVSADDLDRKFRALKASFGAFSSDAIDVVLADSSLILRADTDPLQQPGRQQPQSQRQMKAMSAGGGEYDGRRAPAGGSRGGGGAAAARTSRSGSRSTSIGGDGVSGSALGGVPSVQRRRQQRRDEEQPTTAVTTDIRSGDSHPQGGEEGVEPELRQIVQQSAPSPPQQQQLEPQDLELLWANSVLGAQDTSGSGRGTGAAGGNGGKDMGLLGAMSAADFDRLGSEDLPPPPTLSTRRRRRLLPPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIG9</accession>
    <name>A0ABQ5SIG9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trehalase</fullName>
            <ecNumber evidence="4">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Alpha-trehalose glucohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014451</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001661">
        <property type="entry name" value="Glyco_hydro_37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018232">
        <property type="entry name" value="Glyco_hydro_37_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403:SF1">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01204">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00744">
        <property type="entry name" value="GLHYDRLASE37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00928">
        <property type="entry name" value="TREHALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="555" mass="61187" checksum="4DD06E263B70AE26" modified="2025-10-08" version="1" fragment="single">MATAAASPVKLRARRYILWQEIIKQVSSYGLFKDSKYFVDCPVLRSWDDVSKDWDKLTSEGLTKEILHEFVARNFGLPGSDLKVVLPPGWHEVPPSWLGNVPEPDMRDLGTAVHGLWKTLCRQVSPTVINDTDRHTLLPLPHPFIVPGDRFRECYNWDSYWNIRGLLACELVPAAECLVRNLLHLVDVWGFVPNGTRQYYTNRSQPPLLSAMVLAVWNASNDDSLLVDALPQLVKQHRYWNTGNKAVRVRAGRPGKKEDDERDGCDGIAGGSVKALYEGASEVFTFSRYYAELNEPRPESFREDIQLAAIAGVSGREAAALFCDVASAAESGWDFSSRWFVSGDGLQHTRTTQIVPADLNAWLYRMEQDIAAIAAQLGAAQLQDEYMARASARLHGINAVMWSAEDNCWHDLLLRESCGPMSSAQAQGTAFLSGNTAEGAVYDVELRSGIFASNWVPLWCGCAKPGSALAEAAVIGLQASGLIQPGGLLTSLYRSGEQWDAPNAWPPLVHMIIEAAVESGVPSGTTLANQLADNWLHANLVAWQATGHMHEKYNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SII7</accession>
    <name>A0ABQ5SII7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_014223</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052245">
        <property type="entry name" value="Plant_Stress_Dev_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44191:SF62">
        <property type="entry name" value="OS04G0341900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44191">
        <property type="entry name" value="TRANSCRIPTION FACTOR KUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="54"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="54"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="7">
        <location>
            <begin position="62"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="26"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="254"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="322"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="394" mass="41028" checksum="B6C2106BE4226DE6" modified="2025-10-08" version="1">MSPSAASLASHPLLACEPDRFAAASILLSMSHEEPPQPDVETSDEQHNSSDGANASRKGQPWTEEEHLAFLQGLRKLGKGNWRAIARQFVPSRTPTQVASHAQKHFMRIAGATKRKSRFTTLETEVGAPTKAIQANESNELQHMNRQCPEHPGTLQPASFQGMSAPFPAMPFPLMPPMMFPGPFFPPFLYPPPAFAAAALNTLPKQGHVPMMMPPMFPMAAAMMGMNSFFMAQQMVAAAQAAAANAAAVMQAATSGGGTTAGTGPTATADTANSDEVQRRNQSVAPGSPMICKPEAQHARPSMAVPAAAAAAVASGPALACGAGRNSSSGGTASGGAGESTALDRLRTGGGSLQEHVQYPLSAPGPPRQDDGGSSFHTSSHSAFRPPQEVKSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIP9</accession>
    <name>A0ABQ5SIP9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="308" mass="34389" checksum="F26AD8CD58A27671" modified="2025-10-08" version="1" fragment="single">MPAVSLILSCLDSNARKKLLHATKNNTHTKMSLIYSNPSVVTRQQLMINRLTARRICLHPLGAPRLPHTPVNIPKALPLDDPPGKVVAAALFLGLPASVLLDEVLDTVAPRVNAVTNEESNGVRFFVARPGTGTSTEAWSASTTELGTFPKRRMAVILIHQILGLQKREADLAQALADEEGVVALAPDTFCGQSTSWPFRAIMLGFKYALREGASWSRCTRLSDGSRPSRTLTQIASWLRASAMVVERQSDTRRRIRGRCRVSPSSMAVLCSRRPRWRSWEILLFWGCMGPETRSFRWRCWTNLRRTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJI5</accession>
    <name>A0ABQ5SJI5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013934</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11733">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003520">
        <property type="entry name" value="PRK05183.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="634"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="636"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="656" mass="70303" checksum="635CED00DC9EA06D" modified="2025-10-08" version="1">MLQNGGARALGASILQAVQGSLGLQATALRCLSVSASRLSDAVIGIDLGTTNSCVAVMEGKSPRVIENAEGARTTPSVVAFTDKGERLVGLPAKRQAVTNPTNTVYATKRLIGRQFDDPQTQKEAKMVPYKIIRAKNGDAWVEAGGQQYSPSQMGAFVLTKMKETAEAYLGHPVSKAVITVPAYFNDSQRQATKDAGRIAGLEVLRIINEPTAAALAYGTDKKEGLIAVYDLGGGTFDISILEIMGGVFEVKATNGDTFLGGEDFDNTILNYLVMEFKKESGIDLSKDRLAIQRLREASEKAKCELSSTNSTDINLPFITADASGPKHLNMALTRAKLELLVKDLLERTKQPCYQAMKDAGVQAKDIQEVLLVGGMTRMPKVNEIVKEIFQRDPSKGVNPDEVVAMGAAIQGGVLRGDVKDILLLDVTPLSLGIETLGGVFTRMINRNTTIPTKKSQVFSTAADNQTQVGIKVFQGEREMAADNKLLGQFDLVGVPPAPRGVPQIEVTFDIDANGIVHVSAKDKATGKEQSIRIQSSGGLSEDQIQQMVRDAETYAEKDKTRKEMIEAKNEADTAIYTTEKSLNEYKSKLPQAVVDEITKAIADCRAASQGEDVADLKAKIQALSTASMKIGETLAQQSGSSSSSSSDAGSSDDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJU7</accession>
    <name>A0ABQ5SJU7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF50">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE CMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="346" mass="35495" checksum="C649941D5F23F30F" modified="2025-10-08" version="1" fragment="single">GSGKPPDMVTAAASARADASQEMEEAFVRVGRAIAGLSTLKPEIPVASRGCGGQLQQVAAKGQPRKKKPASTAAAATLRGVLRESYLQVGKRVVGHSVEAFERSLEHAPRCLKVLKLELSKVKAVEASTAPGGGGGVASGCGNEGGGNASGNGGGNTGGSGGGAGSTPSRGVGSGNAGGSGSNGGSGGRNDGGSGRSGEVGSTAASDPHLPILVANHIPFPLGLEDYERVQAVPKFPGACWWDMKGVRGRVPCGAALLPSALRAQLEVEYPQKRKKLNDGSWEIASSYTSSRALRRNNRQPYGRLNPMQAIGTVCGHSRVTNPRKGSVLHWAQDRVMTTREWACIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK25</accession>
    <name>A0ABQ5SK25_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AMP-binding enzyme C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014628</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF32">
        <property type="entry name" value="2-SUCCINYLBENZOATE--COA LIGASE, CHLOROPLASTIC_PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="2">
        <location>
            <begin position="259"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="407" mass="41744" checksum="3209AA0E1D24F3A9" modified="2025-10-08" version="1" fragment="single">GTSLGLRLGLHCVRRILVGAGGTALKVQDAVGRAFPSAELISAYGMTEACSSMTFRYLRGPAAPAASTASGAAPATAAVVMAAARMWGSPGSASSFGSRHVGSGSTPSPGGDLGTEDKSAAEQVTVPASLPAPEVPGAARTELNGAQCVGWPAPGVQILIRPVKDLVAGGSVGADEGPIGRELGGECAPPYVLGEVLTRGPHVMLGYWGDPTASREAFTYDGWLRTGDLGYLAEDGALWLMGRAKDMIKSGGENVFAPQVEAVLSSHPAVAAVAVVGLPDERLGEMVAALVALREGWTFLGPIMPNPNPNPNPNPTPMAPAIVGAGNRAPRVTSLGDLQAHCRAQGMAGFRLPRFAVGQWQPLPTNSSGKVHKAVVREQLAVLRSDMDAERGRTPGTQIGCVAISRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK35</accession>
    <name>A0ABQ5SK35_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051550">
        <property type="entry name" value="SCF-Subunits/Alg-Epimerases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990">
        <property type="entry name" value="F-BOX ONLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990:SF15">
        <property type="entry name" value="F-BOX ONLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Right handed beta helix" evidence="2">
        <location>
            <begin position="346"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Right handed beta helix" evidence="2">
        <location>
            <begin position="432"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="519" mass="54146" checksum="AAE7D0B4E29AC96A" modified="2025-10-08" version="1" fragment="single">TKLVHALEPPMYSQKSSSSGHTRCFFSRLQLRHTRSRPLFRPVLCKFSPSYGKIGNGHDQAEGLNNFKLAGSAASSNSLWPKRQQRLHTRCACGQRGGKSPELHDDAVSTALAADPLDSAAGPPVGHGEGHALTDLTDDRAASGGRATISSTAGAAAVDAVASSDSTIAQPEPFLSRRRALHLTAMLIMTLLPLPVPVVTEPSCTRTRTQGGLLLAPSLVGAPSAAAAMAPLTNVEPQPSSRALGAGGIFCYVSAEPPDGMGSALPAPSPLHFRTLAAAAATCPSGGVVLVVAGRYRERLQLSQPVTLRAWPNGARVEVVWETSEPYQSTLEVNAPAAGRGEVVVEGISIRHSSKSVANNYGVYIRGGSPRLVDCDISSATGAGVGIEGANPQLVGCRIHDCARQGIAVFGSSYDFGLDPAVDPGLMYGMTGGLIRDCDIYGNKLDGVLVRSGASPDLLDNLIHNNAGFGANLQDCSGRYEHNTVYDNARGSVAVSALFDLDPGDLAGSNSLRGLMRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK92</accession>
    <name>A0ABQ5SK92_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05243">
        <property type="entry name" value="SDR_a5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044719">
        <property type="entry name" value="TIC62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47285">
        <property type="entry name" value="PROTEIN TIC 62, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47285:SF1">
        <property type="entry name" value="PROTEIN TIC 62, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="101"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="642"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="642"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="662"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="712"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="726"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="740"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="766"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="855" mass="87721" checksum="4DE06AE63F352372" modified="2025-10-08" version="1">MVLRATGPAVAGSSRSTQGVRVSRSAYGQSFRSRKVTHVRSAVTETDVSETPKGTAVIGGFFAGLTRIGSTGTVQLKSSKEEKKTLATKDGRDDNVVFVAGATGRTGARVVRELLELGFTVRAGARSVEAAESALAVAATYGIIKPDQSRRVTVVPFDLGNVDGFAEAIGNANKVVCAVGAPEDQAFNFSLPKKIDGEGSIALINKAAELGVTQFVLMTSIGTGKLGWPSGILNVFGAVLLWKREAEKALETSGMAYTIVRPGGLERANDDFKRTHNLVLKPRDTIFGGEVSRLQVAELVAAACRNPAVAENKVLELVAEATAAPRSLEELMVEIPQDISREAQLSNKDAVDAARADLAAAQLKASQAAGVLLEADKRVSELAARLKEAKATEAAVKKDVAPLLKQVRPLEAQLADARKKAERAALLEQASKVVLEQARKAAGAGVLLSEKERTEIVEEVLNPKPATSELAAAPTAPQSSAKIFGFFGKKPEPAGPTDEEKAAAAAAEAAAAVEAEAAAAKAKAPVSVFGGLFTPRKIQVDETAAAQAEAAATAAKAEAAAAAAAAAAEAEAAAAKAKAPAPLFGGFFTPKPAARPPAPEPEVVVVKEELVAAVASPSVAEVVVEEPKPLFNVGSFFSGFKKRAEEQPQAEPEHVPEPVPQPAVAAAAAGPTPAKSEGPAPKPAPKPTPPKPAPDAADLADEEQRRRRVVEAAKAQLAAAIAAPSVPEPPAPAPVPAPAPASASAPAPAPAPALASASPKAEEPTPVKAEAPAAPQEAAPVVAEAPKPAKKNDEFDAEDAARRKAEAQAWIAAWKAGSTAGQNGAAAVAAETPAEEEVVEVVANPMSKFFSGLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKH5</accession>
    <name>A0ABQ5SKH5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014646</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF150">
        <property type="entry name" value="MYB-LIKE DNA-BINDING DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="54"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="54"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="676"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="848"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="679"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="703"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="858"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="904" mass="95094" checksum="628EA390F0B7F33A" modified="2025-10-08" version="1" fragment="single">MADELEVRLLAQEDLCWEPDEACSAPEEDSDESDAPAARPPRTSRGSYDLGLPRKRRRRPIWTRQEEAIFARQHCLHGNSWSAIAEHLPGRSAPEVKNIWHSTLRSKKFVARSLLRAYALEVQERGDSTEARMEAYRAAVRRTGGHDNADPDSIDPENALYNNTRRSAGSDGNDGVPVGSRRNPLWRRAFKEEAYTKRRRPAGSSEDEDATSLTSDPSPPQHVALRRDHLISQPVRSQASDPRVAAVFQIGSYPGTLQQTFTRRDPRVRLPDFMSGGDAAANIHAQHSGFHQPPDSLGRDWYDGELDHVAAATLAGGGMAARAAVHPLEARAALLQQLTRQQQLQRLWPDLAAPSNSPGLKGGRQGDVGGGRQEPTGGGAHWLPGDGGGQGDEEGPWHHMDPGHGTADAGLGATGNGALRVAGEELARLQMMVMMQSALERGPPGLKDAAALAVAAGPSAWLPQLPAMATHGSAAGLTQILGQLPWLAQAQATMEAEEVHRLAFLRHQIALQLQQARHLEGAAALGGELLNEEDGGSPTGAWRIREAGGRVAPHLACSVAAAAAADAFGDQAPLPHLPPLGPVGPLAAAAMAAAAPGLNAYALVPLQQHLAASLEQLSALPGAGSLPERLLGAHTTGAASRAAIAEAARAAGAFVPPGLFPATVAGSWEAAPSRLEAQDTGNATGPSSADTLTGMGNLQTPEETGHADGEPRGSHAAVSSAAGGGGGEPRVRVGSKRRGEHRDPSWSASGAKGCAAGTRPPGALGILPLAFLDGSRIDGAGGMYDRYGPFEDAPLPPDRAASFGEAEGFRAISSAAAAILYGARSGTLEGFRGLSGRWDMRRVATEAGFVKPGSGGGRHFEEGDKKRDGASNRKSERRRSHKASPEEEKEEDADFENEKSSEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKP6</accession>
    <name>A0ABQ5SKP6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014693</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ACC deaminase/D-cysteine desulfhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027278">
        <property type="entry name" value="ACCD_DCysDesulf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43780">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43780:SF7">
        <property type="entry name" value="D-CYSTEINE DESULFHYDRASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="609" mass="64301" checksum="CDE928B4918815AF" modified="2025-10-08" version="1">MRCHTFGRAAKSNYAVLPIQEITSLKLKSKCNWELYVLRNARFRPVAHARSAYGKDRERVPSLSLGPTLTDASAALRTFLRHRQWLRTTPAAPLQLVQVPLVTSVQRTLPQPDAAFPTNDSATSCVDSSCASGSLLGTGKGRIPDVLLHSSFNSVGSASPTEPDFVAPLYILRDDLMHPILGGNKVRKLDALVPELLSKGITDVVTCGGVQSAHLAAVAVVAAEAGLQSHLLVRGEPPKIPSGYHLVTRMYGTEVTYVSRAEYANRQAMLSSRMAAVREASPAAKVALVAEGGGDPPALLGLLRLVYWLAYDQTVETGCCQNLTNANALDPRAPGGEERPVRHGSLNRRIATVQSLPYPTTTPYKFVVDSGTGTTAIGLALGIALLGLPWTVNAVMLAGDLSYYKSQQEQLVRGFFEQYGEELFCNSKSASSHPPGRTAAGGGAIVGFAEAAAAAPVNKVTVAEAPTPMSPSVDCGWANEALLPLCWLPRPSPRRFGKVLPGEIAACKLVAQLYGIGLDPIYSLAAWEVASREAVADVVMAMASVSATLPVQAAVATSARDADRGALNIHGCEIGSNCSHVEGSPHVVMLHGGGALGLHGLAQLSPTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKZ5</accession>
    <name>A0ABQ5SKZ5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009836">
        <property type="entry name" value="GRDP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34365">
        <property type="entry name" value="ENOLASE (DUF1399)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34365:SF7">
        <property type="entry name" value="GLYCINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07173">
        <property type="entry name" value="GRDP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="572" mass="63455" checksum="1B0E29B5A943161D" modified="2025-10-08" version="1" fragment="single">IKMESDYYNKLISLHAPASKSVDLLRLLHAVDCFPRGGLYTGYYAIVAATRYERFWVEIVKQNLSFAAPPLDIAFAWFVHRQDPDAYQTAMSGKGVTEVHPPPSKAFAFGPRPSDRKTWKLVAANHPAWPPPAPGSVHSQTYGAFISVTFANQVAASLGRFSQMLKSWLRPHFLNLTFLTQARERYVRFLQLYRDHMDRTLVPTADIALIWHTHLGLSGEYTELCTKMFGKALRPDYLNIADPDDLTTAYGETARLYQEKYGQPYDSPDTAWLADTVLYPLVTAGNPVAYSLNVFDDNPDRERQQMAISLACRGAGLPEPQLGVPSVRSGAHALYLTWLAAHRANESYDDLTCNRCCFTSSASVHKKTIRNVTSALVSIAYFLELPDIDTHPYLLAIVSRAGKWKYSSPLEDVEPAVSVSVPFSPSDAADYLSGRPSHLLKGLTPLLAVAMQPPPPPPPPAALPPQSSGPSATKSSTAAPPVDPWDSPLWRILVAQDMRIKAQDYCTSAWKVSTHRGVGHMQHTYQVRPHKHHGCYVYGPCDYYTVTTLYMADVVYMDPYWSTWDTGGGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLN1</accession>
    <name>A0ABQ5SLN1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="529" mass="56692" checksum="2122A68F8386C28B" modified="2025-10-08" version="1" fragment="single">MHEVMRGGRFGSNDRDTMQSISFSTLVFFWNFDRRLLHGPYCRAAGQALEMFRASDWRFKFALKFEKVARFPGGLPEREALRAGVALMDSARRLLVTDLRLSVCERLYRAFEDEDARRQKEKQQKEAAAAAAAVEDSVAAAAAATAATAATAVAPMPVHVFGAAGADEDGATPAVTGFSTEDRTALSGGDGGGEEARGSPADVALDEGERSREASTPPPPPLQSASFAAKPTGHCSLSAAATVAVSTAATTTCSGGSGGGFTLHQQQQQQQQQQRHHALVPHNSLSQLPEVDDGRRRLSGLLEADRDAYRAARSAAGGGGGAVSNTPAIATASAVPAATAAAVLRPGGPNALDDRDSRGETPHMRERDHAMDGARGYGGCGYGMSGVAGAPEGESASEHPERRGLLSAPLAAVPTPAPPPPLLHVHPRDGDRSRERGGGAGCSSSVYPDRPVDFMRSRDSDRDRSHDRDYRGNDHERSRDRDRDRERDLERSRDRDRDRERGGGERDRLERSGGGERERERDRKDIERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM83</accession>
    <name>A0ABQ5SM83_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000102">
        <property type="protein sequence ID" value="GLI71102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="117" mass="12610" checksum="1E6DAE0DB4A20D8A" modified="2025-10-08" version="1" fragment="single">METKNAGRRDTETNSTKKQCIQFTDALEQLLAARNRCIYTEIGPATQFCSTPTHQSNASNLTMPHLSRDYEAFASAPCWKATDRNVPTASGCSAAQTAAPTFAAAAITDNNFMTSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMN5</accession>
    <name>A0ABQ5SMN5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lactation elevated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_016376</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF6">
        <property type="entry name" value="AFG1-LIKE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="353"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="682"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="368"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="686"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="894" mass="97236" checksum="D3A072E32AFFB5F9" modified="2025-10-08" version="1">MNNTTRRLFMLRVFGGLGVHSPLKLASPVSACICPLTNITITTPCPNPNLGYNIAVTGSRSIRSFSTASTSSQNIDGVQTRTATPFTATITTSNNSSGSNSNVNPVLQRYSQLVESGTLKADAQQLALVQELARLYDEICSYKARMDEYHRGIAEHQARRLKRLRELLSEEAREEEEGERRREGQQAGQVAAARKYERAKTRWRSVASGVGAGSASVPAPLSSLLDLGSWFMNSVVAPLTRGSNGAGRGSAVAASDSPLVRARQAAVRREERLEQEIGPPPSAPEPPKGMYVWGSVGSGKSMLVAMFYQALAENVQLPATRWMHFNAAMLEVHSRLHYLDTERWRLRDERESRHHQHRLQQQQQQQDEGLHEKTCSERTERQFGTQTISQPKTNRHHDDLCTSDMKIHHLGEAGGEEDVEEDGEEVVAATAATAGDRIGMGVVDDISKADLPVPEHLQGVFRHWDPRHDPEVQRQREAKAAMLAVRRHMRLARLGRNDPRTLAAANAQGLVRMAASFIRGRAGDPRSGWGSSSGPIASLLYLDEVQVTDVFSAVALKGLVEALTSAGCVLVCTSNRAPADLPRHGLHEDMWAHFVDTLQQCCTVHELSADNDYRRLILEQGRASIASAAAAAAVTTTSGSGSSAGGGAGALWPPLTRSYILPAGPAGTAELERLWQALAEGAGDGTASSRSYTTVSQQEREEPQRSLSVPVLFGRSLEVRRRSPCGGAAWFGFGELCGRPLGPADYVALSQRFHTVFLEGIPAMSMHVRDQARRFITLVDELYNSRCVLVASAEVAPQELFAGAEGQEPILDLEGMQFETAVEGARLRRDVRSSGGVAPVAASPAALAAAMGALGGAEEQFAFRRAVSRLLEMQSPQYILEALRMRCQQVRIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMQ4</accession>
    <name>A0ABQ5SMQ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016060</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="351"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="483"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="516"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="665"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="161"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="681"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="698" mass="71793" checksum="B3716061E289D9E4" modified="2025-10-08" version="1" fragment="single">VFIRLVVCSVRQLDMCMGCALSTNAAAARVNSTKCGGVHKISGAVYDAPAGDRRVSVRFLKTAEGGVRDEGQKSPPSLNNSSSNSTHFGSVKEDADGKSDVPVQDPLSNGTVSVVGTNGDDSSNSGENGLLRTAGSNSGRVHDDSISGVNGKKPSNGNGGYINSSGNSNRYGNGNMYDNSYENSNGNSNGNGNRSQSSSRNANETPNDDTNSNAHVNANDNSNDGGKLHDTDLSKEVGDTTARTSDSADATANAHTDTKTNESVNGKARETEDRKAKGNFSVNASGNDSPQSQNGRHAGGNGVNSTRVNGTQSTTPQRQGNNMTTNAEGMKHWRTGQSVSEADSPKPPFSLRLARTGRSSESGLLRSGWLQGSADGGLNWGSVCFQTFGVAEATVACRQMALGPLGLPLTASWSGFAAGVPWPLADEMETLLNVQHCTGTESSLEDCARLPWGKQACVGDEGVAGVVCLADIDAVTSAAVHPVRLTGGSSPECGIVEIALGDGLWGTVCVAPSSASSSSSSSTAAASASGSAAPLPSNRTHPQAQKGKPATIAAAASVVCRQLGYTGGWLIDSAIASAYASVPTTAAQRMLPILSGMACQGKESSLLRCDHDGWGDPSACSNAGAGGRMMAVECYGAGSSRPPPAYPPHYHHHLHVPIEAEPSPMIPPTARLLPSYAPVENEPPTPTPRPAPSYAPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN55</accession>
    <name>A0ABQ5SN55_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_016182</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000102">
        <property type="protein sequence ID" value="GLI71109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF21">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="357"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="374"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI71109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="436" mass="47298" checksum="7B7C67A61313AB34" modified="2025-10-08" version="1">MITKYRIHAHNGHAKMLPSGRIGNHACCVLGGARDPARSLDVVRASSIARPTWMTVKPADSRESPFHELDLVDLWDGTGKTRVRQHVNPLRRDYQEPTAPPDWAAVFTDPSLPLALDIGSGYGRFLLLLQRNNPNQQVNYLGMEIRSPLVDRANHWVDRLGLQGRVHYVFANATVSLETLLGSYPGPITDVFIQFPDPHFKRRHHKRRVVQPRLARALRDAMAPGGRLLLQSDVEEVCVAMRNMFERYVGDAFSLATEHSQCAVFFGQPAVSSVPRGASPAPYFSSTGSVNPEGPSGDVTAAADAAALPISSELPAPKAQHQFASPQFVRDPSVHLLDVTQHAVVAVAATDGAEDAAFADGESSGDASTDEDGSASGMDGSSSGGMENDSADIGNMVSEWEKGGWLAENPIGTPTEREHYVMQQGLPVFRLLLVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNN3</accession>
    <name>A0ABQ5SNN3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000102">
        <property type="protein sequence ID" value="GLI71093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12360" checksum="59EB5E17726AC3A4" modified="2025-10-08" version="1" fragment="single">EETPAPPTKKPRAASVRPGITGHEDQPRQVKGAGKSKRGGGQQGRHLAPKGGHVQKKGPKQNLAEYLTTFKCTRREFHFRKQHGMCLRCGSDQHRVDSCPMLGAEKNKGKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPL8</accession>
    <name>A0ABQ5SPL8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">KIF-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016476</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46321:SF1">
        <property type="entry name" value="KIF-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46321">
        <property type="entry name" value="KIF1-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="126"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="18299" checksum="34BE76892DDFB548" modified="2025-10-08" version="1" fragment="single">REEDEAEPYKNKYEAVDLLKNLLGERAISADEPAPSAESSGNESSSVASSSVQQKFASGSPVAGCSSSAVLSTSRPLEAELAEAAVRIRCGLILLETDLLADGQSSIEAGLQPLEKNPEDYLAWLIESYNALGALYSDRGDLSVALEWLQRAEELYRRMTGTGTRHAHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMR6</accession>
    <name>A0ABQ5RMR6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000626</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="5">
        <location>
            <begin position="300"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI58861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="609" mass="64452" checksum="A615B33B0D5372B2" modified="2025-10-08" version="1">MLGQRSLGAHMKGVMPCARPALRPGNQRRSITVKAAASLRTEKFDYIVVGGGTAGCILANKLSADGNKKVLVLEAGPSGDAMEVAVPAGIARLFAHPVLDWGMSSVTQPQLVAREIYLARGRLLGGSSGTNATLYHRGTPADYDSWGLEGWTGKDVLDWFIKAECYADGPRPFHGQSGSMNVEQPRYQNVLHDEFFRAAAAAGVPANPDFNDWSRPQEGYGEFQVAQKKAERADTYRTYLKPAMGRDNLKVVTGARTTKVNVEKGSAGPLARGVEYATQQFGERYTAELAPGGEVLMCAGAVHTPHLLMLSGIGPASALLEHGLDVVANLPGVGANLQDHPAAVLAVRSKPEFEGLSVTSEIYDAKGNIRVGAVIKYLIGRNGPLATTGCDHGAFVRTSAAHSQPDLQMRFVPGCALVADGVKAYVAFGELKKQGCAWPGGITLQLLAIRAKSKGSVGLKAADPFTNPSININYFSDPEDLATLRNGVRFAREIVAQEPLHKLLEEETFPGERIASDTDIEEYVRRTVHSGNALVGTCAMGISPASGAVVSSADLKVFGVQGLRVVDASVLPRIPGGQTGAATVMVAERAAALLLGQTTIAPHREPVCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN64</accession>
    <name>A0ABQ5RN64_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000828</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="183"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="265"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="194"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="333" mass="36256" checksum="8F89F75C71E0FC50" modified="2025-10-08" version="1">MDGLGQLFIAQNGSVYALVPAESREPPPRHVRAENEVMALLAQILQRQQQQQQQQLQQQQQQQQQQQHLRQEQLDMQLESLAAPHAALQREDALHSLLQATLMQSYPPQLQTSLNTNSGPSTSAPLASGPQMPLQRRRLPSVESPSNSGRSDSQSPPPSTRGGDRNERTHHNGLGVSTHLSAKEKNRQAQRRFRERQKDLIANLKHRVEDLQNRVIENEKEIIALKEENAMLRGRIEGKQQGSSVTLPAETLQSLLQMTGAGSILADVPRPKSGGNGNGGGPSNVAGGSGSYRAARSDDMAAAHGAEVGGRGPEVARRGDFKGDFEGRGSDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNG3</accession>
    <name>A0ABQ5RNG3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000772</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15838">
        <property type="entry name" value="NUCLEOLAR PROTEIN OF 40 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15838:SF1">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="277"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="407"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="565"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="622"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="696"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="726"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="763" mass="82308" checksum="FA4C1698427E6283" modified="2025-10-08" version="1">MGKSRSRSPITDRGRWGDPDDRHGYHARSLGLDREDQRRRDRDRHHGSSRSRSRSRSRSPGPESDRGGFRGYKYGTVLDRGGRDRDRGWNLRGQDLDRRRERGRDRGPHRDVDRRHDERRYERGRDRDEARDRSQGQDRERNDQENRPRHQSRSRSRSRDHDRSHHGAQHRGDRDGDLRGTHAAVGVDGGGRGDGGRGDEPRQRRWRSPSPPPVRSLGGVGGGVGGGGGGGGGEEEGLRGRGAWSDLPPQQLQSTDRAVERRGPPPSSLSTAPPPLGSIQRGTVHTVRPFGIFVALPGYRRHVMVHNSQVSDDLRFSKGDDEDAKVKALEFYFPKGEQVWVKVTEVAPDDRNPAEMRVNGSLRVVDQTTGVDLDPEGLLLAEQRAGDAAGGGRGRGGHETDEPPEVGSVHRAVVKRIEAYGVFVALEGYRRHGLVHTSQVSDYLSFTREDTDEQKKAELNGVVTVGEQIWVKVVEVAPDEGRGGFKIGCSIKLVNQAGGEDLDPSNTRYRPRDAPGRGPGGPAGIGSGVGEVGRDGKINWGHLAADIKLYVNEKTAAQYKLLEDEEPAPAPRPPGPPPPGPGGPGLPRSGPMGRGRGMVLPAWMTQQAAAGSAAAAAGIPAAPGSLPANQLPPSSKFELVGGRERDVDPSTLLIDFMGGGGGGGGGSSRITTIEEALAILEAHGGGAGGGKESNKEKKKKKGKKDKKDKKKKKKNKKKSRKKSSGSRRQNGGSSSPSSGSSSGSSSGSSSDDEDNNSRRDRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPP5</accession>
    <name>A0ABQ5RPP5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001002</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047480867" description="Peptidase M11 gametolysin domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="3">
        <location>
            <begin position="244"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="662" mass="73077" checksum="19C317CB9AB0FE11" modified="2025-10-08" version="1">MSPRSFMWSILLLGCAAFLESAYAQPKQAGTVNNGSRVIQATVMFRVRAAYNPPPPPIRQPWLPPPLRTQVRPFPPSPLPTRRPSPKVFSSPRPPSPPPSSPPRSPPPRPPSPRPSPSPPRPPRRPALPPPLKLLPDPVELQIVCPLSVNTGFTRRDPNCNTPIGKLASGKAADELIKRYGIMSGDKAIALIQSSPEPTSSRRLLVGQPPALTYSLVEDPLFVTHGGQKEIYQGTTIDLITIIFRLDFGNCSLNSGWRRLPAPAMMNAIYINSSLPNNLQNYYKTCLRGKVRMSDENTFVVKTAINITCSGVMVRDGLPDTPWNSSSSCDVAEQQAWEDQTVEQANEIAKTDPALSFALRQFPRRRHIYILPPSVPCPWAGFADVTCTAFFCSSFLRVGAYNTLSSTDLNVIMHEAMHNFGLEHSETERDPYGDPTDVMGNFGSRDGLLCPCAANLYRIGIAMPVRGNWDEDVRGMYGNLTASNFTKTGQVTIVIPAAFSREDHMVVVNLGRINSTYSFISSFRTDIPIFYLSYRVASPDIAGYDSGLRPTENRTVIVHTYKGIQDEVLSNGVRPQYITSIPCNGTWSSDFYNFSSDTGGKLKIKVKSTNDTLAEVVLCRARAATEIDCSDDLDDDCNGYTDRCDPLCKPSIRMRSCPKSIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPU7</accession>
    <name>A0ABQ5RPU7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044176">
        <property type="entry name" value="TRL4_chloroplastic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47912">
        <property type="entry name" value="THIOREDOXIN-LIKE 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47912:SF1">
        <property type="entry name" value="THIOREDOXIN-LIKE 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="56"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="214" mass="23932" checksum="6506C50BEA2E108B" modified="2025-10-08" version="1">MAVSGLGLKCPSRNLWTTEAQAWVPGKAVCPGRRAVVRTTALHIDRRGALEAAWRRQEEAEARKQRLSTDIGEGKLITVPDTAGLDAVIEQAGSQLVVVFMYSRQCGVCKDAARRFEQLRNEANRAKARVVFVQHDVETDYGDKSDLSRFYNVRAVPCFLFFDGGAVVRRLSLRDIRRLTGPKPYVQAALAEDVRRLRNTFMEVLLSRAPSARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPW6</accession>
    <name>A0ABQ5RPW6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">TFIIB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_001544</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="159"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="159"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI59612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="47857" checksum="2AD994A5A50E27D3" modified="2025-10-08" version="1">MDAEAELCTRCGTHSVSYDYGASCCVCQNCGLVPPQQDLVEYREIADPQQGNAVGGHFAVKAAYGTKGIQPHATGVAAVLHPADSDGANARARRQRRELDDLERLAAILRLPGPVALLAAGFLARIHEAASEAGAARRYGNDEAQVEAEASAVVKHYGTSDSWAGPSTGQGNADGSGDAFPDPTAGDPRSLIAPRPFTGGQQSSARLGALLYLAARSERGTLTLAEVAMATCTDMFRVGEMARQQAAFLRLSLPSVDVERFVMRMARELVASGQMSQRPGTAAAEATAGVAAETEVGREAAEASPELTATMPVSATALWSHPLVCKTSQLTELVVRKGWHEGRSPRTVAGALLSICLDALSPQREVAAAGAAARMAAGGSGASGINASGTNRSPSTAQLSRTTMCAASQLLELKQEILGHLVRLAATLPFGPLVGRSNVATYLDVLIAADTVMEGIQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ89</accession>
    <name>A0ABQ5RQ89_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="272" mass="28691" checksum="3DFAA9145C53AF77" modified="2025-10-08" version="1" fragment="single">GGGGGGGHPPIESLARITSAPPPGLQNELSYGRLSSDHSHTNQMAHAKGMSYSLPYISSPGHHMSVSSLPPGWGSSMGDGSIHGMDSFSLDHMGSISIHSGLSAPDRGLTGADAGGAGSYFRCRSLQGLSNGGAGPPGDDHFRQTAFFNHPGVSQQQQQQQQQQQQQQGHQSQQPQHHAYGAQLPCRQLNQSSPQQQQQHLQRFSQSASSQHLQPPHQGMTAAQGPTPEQQQQLQQQLQQQQQLFAQLFPGAQQVVGGGAGGVVVLSPSAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQJ9</accession>
    <name>A0ABQ5RQJ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_001410</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
            <location evidence="2">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021911">
        <property type="entry name" value="ATAD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23075:SF0">
        <property type="entry name" value="ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23075">
        <property type="entry name" value="PUTATIVE ATP-ASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12037">
        <property type="entry name" value="ATAD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046225638" description="AAA+ ATPase domain-containing protein" evidence="12">
        <location>
            <begin position="31"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="351"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="602"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="83"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI59524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="625" mass="69226" checksum="04928A7FDD47E60E" modified="2025-10-08" version="1">MAQHARRLAAGLWLSSGVAMAAYGSPHVLAEKDDKDKVANVHNDRLFDPEALERGAKALRDINKSPYAKQALELSRQQEITKQTEHKEKEADYRRQAAALEKEREIVRYEEERKMEEQRAQVAARMKQYEDELARKRMMAEHELQRQRNAELAKLQEEASVRAEQERLRIEQQIQAERRAAEQYAADMQKQIQRERALAEAEGRIKEARENEDVNRRAALLKYQEETRKAIESINAVMSHLGNAALELVTDTNKLLTAVGGTTLLFLGIYATRETTRVVGRTVESWLGTPRLIRETSRFNLWNPKSWSLGATKTKEDIKRDFSDIILHHELHDTVRQVAAASANTKAHGAPFRHMLFYGPPGTGKTMVAKRMARTSGMDYAIMSGGDVAPLEGKAVTQLHQTFDWAEKSRRGLLLFIDEADAFLGRRSDAMSEGLRGSLNALLFRTGDQSRDFMVVLATNRPGDLDDAVLDRMDEALEFGLPGLGERQRLLGLYLDKYIAKAGTAEGGAGAGAAGGPLARLTAILKGRKASADRITISEGITEELLAEAAKATEGFSGRELAKLVAAVQAAVYGAPQPVLTPEIWRTVLERKLHEHAERRAFRLGHHGTGDSAAGSAGAGSGKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQQ0</accession>
    <name>A0ABQ5RQQ0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_001796</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Fanconi anemia protein FANCD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029448">
        <property type="entry name" value="FANCD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32086">
        <property type="entry name" value="FANCONI ANEMIA GROUP D2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32086:SF0">
        <property type="entry name" value="FANCONI ANEMIA GROUP D2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14631">
        <property type="entry name" value="FancD2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1077"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1142"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1321"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1490"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1784"/>
            <end position="2040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1081"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1145"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1321"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1493"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1790"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1904"/>
            <end position="1919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1968"/>
            <end position="1989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="2026"/>
            <end position="2040"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="2040"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI59881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2040" mass="206440" checksum="AB365417D26877F7" modified="2025-10-08" version="1" fragment="single">MASRQERGSLHDISNVGARHTGAPGKPSKSPPGKAQMFIRLIEAGGVMGWTTSEDTVLPGSLEVADPRRLRSYLESRLQRDPALREQWLEALQEACEDEEVLHRALVPMSLAVAPSVGSGPGSGTYLGGGVGGGGGGGSCGDSFMRCVLGVACLQASVATWLLQRLPVLAMAEPAAGTSVSGALDTCATGGVPGLILGQLRWLDVLPDAGGDGASSLVDVVLEVLPLTPPAVQRQLIGLLPELASVDDHARIVEKLGELLDSELGFVAPTLEALQQLQLGPELQDQVVLDMAQRLPGVDSDDLPALVRFVVQYSTGPPATAGIGARGGGGGSVAVSAAAATAPSVFAVLRGGLPMVDLNDPRVNVPDPKGKRGGGSRQEPVEVRILRELVVALTANDAAAGAVLRAISAAGSGVRMGPGGSSAPDLDPVTADIDDALEENLDSEGRDSAAAAAALADGPQKLVPLDLWILLALLGGRRGKEADKILRAKICDGTASPSWLSSVLLRHSAALREMWSAAMSLSATLASSNPSGGPLATASARLYAVMFTAMSGQEDSLQRIEVLHALHSHLGSGVREEVDTALGALAQLAEGPLSRSSSGAVAVSAAAGGGPSAPALSLYGSALSNCLDHLESFTDSQLARLFDVLAAVTGGAEGVAWRAATEARSAAASDGAAGGRGAVVRIAGGAGGRLESEVQIVVDKALKGSASYKRIGIAGSLAFLRRVGCALMLLDGGCGGGGGSGGSSIAGGGDGFDLLLREWRGRLDDLIAATEGHGAARALALGLISEMIKTPAITTTATATAGAKAQAPVTPLAGCWGAKLPAAALEQLTAVVQTELLERALVVDLPLAAAPASGHEGDGGPRKVRVAGCAVPFSCSVWLNVDEQETSVAINLWPLAASSAITDRDVLVWMAPCLSLVCLVSRIVFGDLQDVDGLLGCPLVMFPMELLQDDEGINHLHELPPVVSAAVLTCLQAASSWLKEIINCFSPALAAQHQGGGWGESGTAKLAKRSTQLAVLEALAAALVDNIPPPRGGLAALQALRSHADPLGRFQKCGAGNGGAARSSAAAVIGGVKRGGRAAAGDGGGGGGTAGGSGSSGGGGLTNRTAAVLGFRRCVRPLSLSGTVETLRHLLTACPPGVYGGSAEANRRDGGGGGKGDDDGGEDEMLDPRNWAAWTVLPIRLAAASYLISELAAKAAVVAARARNAPAFALSGSAVVAAADTSLEGMSLGKFAVTLAGVMPYVRKHLDSATAALAGARILTASNNTYLQPTSDGDGQRLLTQLDAVQAPLESSYTAAQYVMNTLDLLRIFFGAFAPPGAGGGGGAAGRGRGRAAGGGGAAAGSGVTPSPQVLAAVLACLAAKPATEGAPDADLKKLAAAACPAAPGRALNSAQGEGLALLALRAFQQLTSQCQGAGLANPGGSELAPQNPPASPDVWRELALLRTGRALIAAVRHLVAPDVASAAAGGWPDGLRRLAKAVSRSANKALENDWSGSGGAAAAGGGGGGGDDDGDGGAANDDGDLAAASGGRSGGAGGSFRWRGQAKLILEFLRIYVDLSTKPLDVLKLLVTQAMSEVPAGKAGGGRGAAAAVVSEPIAGYASLGDATLAAWYRGVFEALLERWSATCAQMRASERRVLGQEEEEQLMVETLSAAEVFSKLMELVKKHPGRTPLITAAIKCGGKFVEDLARSTGAWRRLWEAHEDKLRRTVKSVQRGTRLLNTLCAEGKERRAVALAAKVPAVKRAMETFLLNMRALFHEMGCADGIRIAQLKNKDIHGNEVASQAYGGYNDADADGGGDDGGGEDRDEGEEHNDAGANAAPADGHDARRNGRKKLKGAGEAGHGKRVAGGAAATEGDGGERDGAPAGPKAKKPRAPRAPKEELQRTGSEAAAPRARKLREPRPHADCAAGGVSGDVGADAAGRVKSEEVPEADIGPSALLNKVGKRPRTATDAKSRRKMMKAAADCATGCNGGGGSGGSRGGGGNSGGRGRTLGVPSQTRGAGSQHRLARVAEEEAEDDDVGRGNGEVEAEAEDEDGEEEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQT3</accession>
    <name>A0ABQ5RQT3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001974</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="36613" checksum="4ACB208387E3C334" modified="2025-10-08" version="1">MPLCFELIPFSAVSAGVSSSPNLPQVIPRSQGDSQCVNFIRSPFFGLFRAGYANLVLINVHLAADKEAARSEVSVLNSLAEAIMKPNERLLRRHFLGGRLKGRKPRGLVSILGDFNCPLHVKKAPVTTTTITSAATSGASTSTATTTTPGTATTAALAGPGPVAESRNPQRQQRAAGVSGAGPSSSAATTAAAARTSTPEDNRQNDNSDGGGTSGVGAPTMTNESSWDDFLSNGWFNALCFGNSASSNGRPLPTNWWARKLKDLDAICLQQAHGGLVKGSGICAPPPDVEPSQYPNHLLCWVLLDLAPLQGAPGKVRPLLEPAWPPGMAVPAVPAAASASGAASASGAMATGGSLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRD3</accession>
    <name>A0ABQ5RRD3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MIF4G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001598</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007193">
        <property type="entry name" value="Upf2/Nmd2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04050">
        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="5">
        <location>
            <begin position="48"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="5">
        <location>
            <begin position="543"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="5">
        <location>
            <begin position="750"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="366"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="419"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="981"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1058"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1147"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="367"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="420"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="446"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="461"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="999"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1023"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1076"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1150"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1165"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1209"/>
            <end position="1229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1236" mass="131378" checksum="80C261218491AFD2" modified="2025-10-08" version="1">MDVEALQEVIAQLVVTRKSLEAQRLLREGNLDAIRPSEDHLKQLDSSIKRNSTLTKRLRQLNEDNKQTILDDIRKTNQSKYVSEAVAAIAEAPLRNVDVPAAVAVCSLLHQRYADFGPQIAAALCKAFSKPGGGGAGADGGGGSGSAADRDGAKRRRAALRLLGELLAGGVCIGAGPLLSILKDLALPDYRNDRDAAVTNLTLLGTFARTARELLLGQPSAAVLAASAAVPKQLMEQVESGGLPEGVGAALAQLKAEQQHLQEELGRRFVLPEAEAAVLTKILDRALEAAMAALSEDHRQLRDLEAENSALVNTRGDLPADAAAEYERRRKVYEALHRAVAALADGLGKEMPELAEDAFTRLGVGDSSGTAAKDASTSGAQGGAGDSALEPVFEDPDARSFYESLPDLRVLVPSVLLFGDSKDPRGRNEVDQQQQQTPGDKEKAKGSGGGGGGGGGGGMVEGVEAAAAVSSSARDAAPDGGADAAADAEVTPLPAVDSSGTAAATETEADGSTAVAVAAGADDDAGGGSSAGGGGGDAGGAMELIINRLPSCVSRDLADDLAVNFCYCNNKANRRRLVRALVDVPRSSLQLVPFFARIVAVLAQVLPDIPQGVIAQIDSSYSQLLKRKDPTLTAASLEPRLRTARYMAELAKFRLLPPGPFFSALKQLLDDFTGHNIDTACTLVEGAGRFMYRCPETRTRMENMLEVMMRLKNARNLDPRHAGLVDSAYFAVKPPSKERQRRRKRSVIHEYIRHLIFEVLSEATIPVVLRKLLKLPWAQYEKYVVKCLLKVVRGRFSNIPLVSCLAAGLTQYHDSCGVALVDCVLEDVRCGLENPGAGMYQKRMADVRLLGELYNYMLCNSTPVFETLYLLVTFGHEVPELAERLDPPDDFFRVRLVCALLDACGQYFGEGAARARLDRFLTFFQAYILSKAALPLDVEFDLQDILHALRPTLKRYGTYEEALAAVQDIIAREVAQFGAPLGTIGEEEDEEEERRVAGGASGRDGGAAGNETDDGAALQGEARSLDEISGRSSSDERDELDEDFDREWQALVGETQSRLGGIPLAPTRPPVAASSSGGGAEADGGGGGGGGSVTLRMLVRRGGKDDRSKELQVPLSSSVALAVRQREEEEAREREEMKRLVLAANRQQEEQAASLEQLQLGVKSGGKRSGHRQAKHTGQLDMSVLDDFGPSGDVQYERTVGVGPTAQQTGGGYGGRGGGRGGQGQARSGGHGRGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRY7</accession>
    <name>A0ABQ5RRY7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase</fullName>
            <ecNumber evidence="3 12">2.4.1.131</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_002460</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:29523"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="EC" id="2.4.1.131"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11 12">
            <dbReference type="Rhea" id="RHEA:29524"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.131" evidence="3 12"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03806">
        <property type="entry name" value="GT4_ALG11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038013">
        <property type="entry name" value="ALG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031814">
        <property type="entry name" value="ALG11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919:SF1">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15924">
        <property type="entry name" value="ALG11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="ALG11 mannosyltransferase N-terminal" evidence="14">
        <location>
            <begin position="39"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="13">
        <location>
            <begin position="269"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF15924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI60340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="474" mass="52636" checksum="AA37D1F29DADB094" modified="2025-10-08" version="1">MDSWTMVILVTLFAIFLATVTATAVFFVVAGSLPRVRKGVVAFFHPFADGGGGGERVLWCAVKAVQVACPKAQVAIYAREGVTAEQLVRDARSRFNIDIEKPIQVIPLRRTHLVLPERYPRFTLLRQALGSVVLGIEALRQLVPEVYIDTTGWAFPYPFARKAGSRVVAYVHYPTISSDMLRRVWRGTATYNNDADIAESPVKTSIKLLYYHAVAWWYGLCGCATSVTCVNSSWTRDHIQRVWWLGRAPVLVYPPVDTTELLQLPLDRKLKQLYLVSVNQFRPEKNHRLQLEAFALAKRAAGPTLQGQAVREAKLKFIGGCRHADDEARLAALQQYAEDLGLAECVEWIVNAPFVELKAQLGGAVGGLHTMTDEHFGISVVEYMAAGVVPIAHRSAGPKQDIIRPLLEDDSSEQVTGFLAITVQEYADAITRVLTMDQRERLKIAGAAQRHAKSYSTERFLQDFTAAVAPVLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS14</accession>
    <name>A0ABQ5RS14_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Homoserine kinase</fullName>
            <ecNumber evidence="3">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_001779</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-homoserine + ATP = O-phospho-L-homoserine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13986"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002288">
        <property type="entry name" value="PRK01212.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF1">
        <property type="entry name" value="HOMOSERINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00958">
        <property type="entry name" value="HOMSERKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="12">
        <location>
            <begin position="122"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="13">
        <location>
            <begin position="266"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI59812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="367" mass="37447" checksum="94BB32A0E06E9140" modified="2025-10-08" version="1">MALHQNLQQRQKSSCTCKSSCRRAVVRRPARCTRLNVAAGGKVYLDPVRKECAAFAPATVANLGPGFDWMGCAVQGGGDTVIAKVLPGRAGEVVIEAIQGDGGRLSLDPAKNCIGVAATETLKLMSGAVSCGVSLTLHKGLPLGSGMGSSAASAAAAAWAVNGIFGAPVPKDKLILAGLASEAAVSGYHADNVGPSLLGGFVLIRSCKPGEPVELLQLPFAAADRLHFVLVNPCFEAPTAEMRAVLPKEVPMKSMINNCCQGGALVAGILRGDVKLIGESLDRDVIIEPVRGPLIPGMLAVKEAAKAAGAYGCTISGAGPTAVAIVDDPEVGERVAAAMSGAFRSAGKLEVNTAQVVKLDTEGAKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSR8</accession>
    <name>A0ABQ5RSR8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rad21/Rec8-like protein C-terminal eukaryotic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000006">
        <property type="protein sequence ID" value="GLI60283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="2">
        <location>
            <begin position="118"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="163" mass="17116" checksum="E59A1F8321E314A1" modified="2025-10-08" version="1" fragment="single">NSKGGGRSEGASGGVSRKSSHQAGSSARGGSGFEKEAADATPFFCLKETNGLATQVLPPQLFMSRNTKVVISIFRKQLAQAQSQAPVQVAEQHQGAGHSNGGDSGISDLQEPALSFFGLASRLKRSEAVQLFYQTLVTHTAGYVVASQYEPYGDITIRAAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU43</accession>
    <name>A0ABQ5RU43_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003317</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050673">
        <property type="entry name" value="Mito_inner_translocase_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13172">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0811">Translocation</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="9">
        <location>
            <begin position="23"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="11860" checksum="008E9911759E7176" modified="2025-10-08" version="1">MNFPLDSVQDLPDDAKMALGAALEQMQVRDSLKMYNRLVERCFKECVEDMRSKALTAKEEQCVAKCCEKFMHVTARVGMRFQEFFSQMEQQAAAAAAAAAAAGSQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUA9</accession>
    <name>A0ABQ5RUA9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="9">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_003405</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="9"/>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059090">
        <property type="entry name" value="ALA1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26023">
        <property type="entry name" value="ALA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="11">
        <location>
            <begin position="221"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1128"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="810"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="814"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="929"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="933"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI61130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1165" mass="124204" checksum="A7A6DC6060441BB1" modified="2025-10-08" version="1">MVDAKPRLYGVGVKALRLQVVAAVAGVELDVPPFTEGLTNQTPAYLALDAEGTFPLLVTSAGTPLASAGPALKELLGSSVAAAKWADWSATADVFVPGWINGLLGLAQVDDAAAVSAANGFRAHLNLLETDLATSRFATLGGGAAVDAAVAMSLFVFYLTVFDENLRAEFPKTGAFLATVYASPAVQAALGGVEVAPPAAVLQSGDASPLAAFDSVLTQPWSGARTRAAFNEFFETKAHTYWASGSVVPLNDPTLLFTNAGMNQFKPVFLGTVDPNSDMGKLKRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKEEAISWAWELLTKWYNLPADRLYATYFRGDTSQGLPADDEAREIWLRFLPESRVLPYGNKENFWEMGDQGPCGPCTEIHFDRIGGRDAADLVNADDPNVLEIWNNVFIQFNREPDGSLKTLPAKHVDTGMGLERITSVLQGKMSNYATDLFGPIFDVIQQVTGARSYTDKIGKDDVDGVDMAYRVVADHIRTLSFSIADGARPGNEGRDYVLRRILRRAVRYGREKLGAKEGFFAGLVDVVVANFGGFFPELVKQRDTIYSVLREEEAAFSRTLVKGIERFKKAALAATDNKVPGYEAFVLWDTYGFPVDLTQLMAEERGMTVDMPAYEAALNEAREKSRQGGKKTAGVGIKFEAEATGWLQAKGVPLTNDAPKYGNSDVHAKVLAILTPGGFVTSTAEAADPDGPMGLVLDITSFYAESGGQVGDTGAIRGPEGAALVVTDCQVAAGFVLHVGEASVAPFRVGDEVTVSVEYERRGLIKPNHTFTHVLNFALKSILGDHVDQKGSIVLPDKLRFDFSNAGPVETGQLAAVEKICREAVLASMPVFSGEVPLAQARAINGLRAVFGEVYPDPVRVVSIGRAVDELLVDPRDAANADFAIEFCGGTHLSNTSEAEAFALLSEEGIAKGIRRIVAVTRNEAVKAIEEAARLKHELAGIAALPDDQLEKAIKAFKETVDAAMIPAVDKAALRDELGVLGRRVVEFQKAAAAANKALAAERAVAVADAVVAAGKTFVVDRLDVGLDTRAIGEACSAISAKHPLLAALFISVDSEKGKALAYASVPDVATDKLKANEWVSTALGPLGGKGGGKANNAQGQGPNVEKAEEALAAATAFAESKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUC6</accession>
    <name>A0ABQ5RUC6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BRCA2 OB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003482</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015525">
        <property type="entry name" value="BRCA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015252">
        <property type="entry name" value="BRCA2_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036315">
        <property type="entry name" value="BRCA2_hlx_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015187">
        <property type="entry name" value="BRCA2_OB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002093">
        <property type="entry name" value="BRCA2_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289:SF0">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN BRCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09169">
        <property type="entry name" value="BRCA-2_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09103">
        <property type="entry name" value="BRCA-2_OB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00634">
        <property type="entry name" value="BRCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81872">
        <property type="entry name" value="BRCA2 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50138">
        <property type="entry name" value="BRCA2_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Breast cancer type 2 susceptibility protein helical" evidence="8">
        <location>
            <begin position="1084"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="domain" description="BRCA2 OB1" evidence="7">
        <location>
            <begin position="1137"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="516"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="730"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1016"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="546"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="745"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="810"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1314" mass="130992" checksum="FEFAD652D1C3E1A5" modified="2025-10-08" version="1" fragment="single">CSGAAPSGSVTVVLPEKVSLTGFAAPPVGQDGNPPAATVEISEGGFSTAGGKPIMVSEEARQRAAGWLANVLINNEDGGSEDRGGDVGDGMLGDVRSSGPGDPGGAPSAMTTCVVSGKAAGGAVGEAARMGPPGMGASEAFGPAVAIPRGSAVGFSTAGGKPVIVSEAARKRAEGWLAKIMNDDDEGASGGGAPALAAAVRAAANVPETLAQPGPALRRPHSDGVPYRPPARRRVASLGPDTVAAHLDTSTTTDAAAAAAAGTCLPPTSPLPDAQAPQLRAMPQRGSEAGLGCLNVAFAAPSELGCRLLPAASIVALPPAAIALPRSGTTFPSGRPVVLGKGASMYAAAGAERKSGSESGAPAEAAAAEAKVPAGRIQLVTAMSTDTATNMGALAADVAAEAPAGATAGGRTAGGEENFILAGRGQQPSAAGAVPAFAAAADVCSGDADPSAGDRAAVADGAGVGTLAAYGATAGPQASAEKQPTGFVTGDGNAVLVDPARLAAAAALLQVVGSDAAAVSPSGTRKTREQAFGGEAEPQGAGPHGQPQAHLLAQQQQQQLVEQQQQQPSATTSPVALQVTVRAVANTSLSPDLMAAAGGQMSAVGEEHRGPVEAKTPSPPAAAAAAATATVAKAVEDAAANTRERTRTAAQMRSAVGMSDTVVAGGDNLDVQAGVVHPPPAPRQPVPAFPFCATAATTPISTKSTMKSTDGSELNHVAVADIPFGTAAASGAEQHTPLRTLSPQPTEAAAAVVAATASAASTPRATTPRMRPVTTTPGSVPRAGPPPSTGGLKRPRTLANSGSGSGLTHGSGRPPRHGGAATAPPAAGLGSKRKFVSPLPAGYKGKPGSADCSTPLRGDTPAAASRAAWDGSDSAKRRSGQGSPKRPRLSSLFELEAGTGGRISKLPLQKYFTLPLASVAGASPPTSPGPHRAAVVGGEVPIAAGEVGGNIAGAGDAPPIGDGGAAVGAMADADALAVAVAAAAPSHFTPVAPGVLDGITSATAAEFRFWAALEAPSPQPLSQPEDPKDVRSMDTGPVGAAQTAEGGGTCAAALVWGWWGAAEARAALQRLVALPLSARERATDPWVTNHYRWIVWKLAAYERRFPGHCRGRSLTPAAVLDQIRRRVQVELVGGGRPILAAIFAHDRPAGLPMVLAISGLPELSSRSHFTEGMRVELTDGWYSISAGLDSHLAHLVLSRRLVLGSKLRVCGAELQSERPGEPLDSATRSSSVLRLQYNGCSPAAWDARLGAQVQPAAMALRPLGQVVPGGGTVPATLLLVVSKYGILTYENTPQGIFNRTPRTQEERLRSSTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV77</accession>
    <name>A0ABQ5RV77_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003397</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF31">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="618"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="653"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="748"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="812"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="817"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="862" mass="93244" checksum="5F321BCE748F4009" modified="2025-10-08" version="1">MRQVLRVRHRTPTVLVRVFTATETGPSAQCASIICYEKLLGSEVGDQNCGCVPCSSGRDANPSSLIPRVFMFWSRWDLQLPIFLSEDRFARKLRSHTGPQPWAAHTGNHATAETSNASHPCWPAGSLTGSVQLSSSNLPTRHNGHTACPGTMTVPYNLLPMLVHHRPYCQMAARPVGGLHSVKSTRIKKTSAGEGCAPQALGGSGGGDPIAAADSEATQPPSASRSTVGGVYGAISSTSSSPSSPLVRQLATQRHVLRSGASVQNVCVQEILGRAKMNTVFQVIVIRSDGRTLEPNGGVTASDLGLHSRDLSLFARDSRLSPQRATITVRGDRILFRTEAVKAVIERDQCTLIRNKRDRDFHDIIKPMTLVIEAQPQVPFELSVLEVLFHVTLLYFERRRSHVRWMVDRIVGDATGGFLEEGHSGAGAEGTLGTGDPFGGIGESTVQQFAPLEKVLTAVLNDARETCDAINRFLDDDREVLGTLLLSLNSYTPPPPSLHLPPHGLAAPVEATSVAVAGTVDGALPNVSGGVAAAVSAAAEPTEPSRRGFGVITPTIGMDPHQRREMLRNQAIEDASRILETYLREIQSIVGSLLESEDYIESTRETYRMQLDSARNHIILVNLWISVASISLMVATLPSAFFGMNVQHGLEEVWIAFPVIVAMSLGLAVGSYPTLLRMFTERFLRQTRHDTRNLMMLRAFLMQHSDDLEAITEALRSLPCVSHEERGSVSREAFRSHMRHRLPSHVTLSDSHLDYLFRQFDKDNDGVLNEAEATLQSKRSYPGAGGSGGRGASRRNRLGDMEWGWGGFGAVVGSDGEPLDGSDEGEGEGEGDFGRRSRGAELAPASPQRASGAFGATSRRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV91</accession>
    <name>A0ABQ5RV91_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Deoxyhypusine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003937</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="42573" checksum="0B3ED334A305BE8F" modified="2025-10-08" version="1">MAHQGCADIPAAEAVLVPTEILPDTPIVRGYDFKNGRDLDGIMDSMLTTGFQATSFGQAVLEVNRMINWRLSDEPVGPSTDPEHVDPGFRASTRCLIFLGFTSNLTSAGVREHIRYLVQNRMVDVLVTTAGGIEEDFIKCMGHTYLGDFQLKGAELRMKGLNRIGNMLVPNSNYCKFEDWIIPILDTVLKEQNELGVNWTPSKLIDRLGKEIDNPDSIYYWAHKNGIPVFCPAITDGSIGDMLFFHSYKSPGLRVDVVEDIRRINDIAMRAAPRKTGMIILGGGVPKHHICNANLMRNGADFAVYVNTAQEFDGSDSGARPDEAVSWGKIRVDAKPIKVCGDATILFPLLISQTFARHWRPCEPLRKAFPEKAAENCVVKATFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVQ7</accession>
    <name>A0ABQ5RVQ7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058921">
        <property type="entry name" value="PAP/OAS1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45979:SF30">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45979">
        <property type="entry name" value="PAP/OAS1 SUBSTRATE-BINDING DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="892"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1068"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1243"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1361"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1504"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2444"/>
            <end position="2472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2673"/>
            <end position="2746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2775"/>
            <end position="2825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3125"/>
            <end position="3152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3231"/>
            <end position="3254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3293"/>
            <end position="3329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3359"/>
            <end position="3386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="662"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="712"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="906"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1259"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1374"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1427"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1522"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1535"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2453"/>
            <end position="2472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2673"/>
            <end position="2682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2711"/>
            <end position="2727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2728"/>
            <end position="2746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2775"/>
            <end position="2788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2800"/>
            <end position="2825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="3133"/>
            <end position="3143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3232"/>
            <end position="3254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3293"/>
            <end position="3306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3374"/>
            <end position="3386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3448" mass="362434" checksum="4D8E20222F05F175" modified="2025-10-08" version="1">MPKQSTASEDYNPWKNVLPIRSVDVDDLTLQEQLAKCIVPRKRNTDKGRSKPAKTICPNLAKFAATLPPIWLLCGQPPRRSRVVGAHDAQSQNIVVCCTCEWCLKQNAAEEGASSSRGGRQQRQAGAEDSLFQGRLFNLPQWHEHCTDTRITGIDVTKKPDQYRRLLFNVVHVASEPQADGQGRSSQQHRRLETNKTLWSFLLGLSKAEERAVAAAVHAQGPRTGLQWQPERPIVVKGEYTGLQTGQDHLATGSAAMRHSLTGGSDLAGLHEACQDGRLPKCWLEGLRLHVFYHEELPVGRWYSGTVLRSSQESPVLQVEWDPWIHEPTNTQQEATLGEVLLGITLLHFGPAPPSGNGPDTTDRVFTLPSRSLVVEGRTGGAAGPSLPAGDASPIGRVQRRTSPSATDGGNAAGSGASDLDALAPSQLVQRLALLGAKGSASRQPIPEGHRSNGDTKKRRRHAQEQGARIQKLGLQMGQLLEGLVIGRDAKAAPEGEEQCTALDVLQPKAKALQWTEQLSRKKRHAPSTAMAPCNEPAPQKNHERRKSPQTPQAPPLIKQDLLEESAPTGLLHSRQDQQRQVQSRETETQPLQPQTLVSLLEQSQQGHRKVNPVVPSSDLPGRPEGILRDSHTAAVGGGEALLRDQNVPSRLTKRQRAETPQHAQNASHSQSQHTSPQGGKKRPKVAQMSTKPPIPIPPGQKQLPKEQACLQPHQGGPNEPQQQQLLQGQEAQGPQQPLVASEDQSMNTIELALLLAQRRGLRPPSPPAEAGCVGAPARASAGDGGDQGAAGCTRCVAQGAGMDPSAVPEHQSIDAHSPGPAGIIPAISSPPQPVDIMHASGLISGRIVTEQNNHQVRDGGGSFGAPDAPAGSVGCITAARTTQSGFVLPFESEPQGRMRPPQLGEAERHKDQHQHGDDLRPEAAPPGFVLGAGNFVSCVISTPQPDVGKLNPLVGESQQLELIFSQQDTFGKDLNPGSAGGVTSDAGATLCEAGMGRNVAARSSRAKIPCPANADALRLAGALDAVSSGVEAQETANLAASAAPVPTAACTGLPELQSMLPALELRAPASDGEDGGPPLVPDDGSPDGGEEPRYAMRAPSSPSADLMQGLAAAPRSHCAAGLYQAELTSAIAKKVGRCHGAGLGVDGNGSGRSVCVHGGGEPFGRGFAGSGYCSNSDGDLEDEHDHNSRRDEVGRRVRYLLTVIGANSSSAETTCSEGHGAVVEATGEGPRASLQLHKVTAPAPQLPAPSPSHDAKSTPRTLNASSSPDPDQVSIADGGPAPAVDAAAVARMRTPGAGPASTNPSGCTVTLPEVLGSLQSQGFWSRRPVTRWATHMLPPRPHLSVLAGATGTAAVAASSVRDDYDLQTDDETRDSSSYRTAEEASGDDSGGDDPGLASTTRDAGKAQNAVVAHGAKGQDEGVPGVQQQQQQQQKQQQHVAAADFERVAHDEVGPDPQPSTQRHVRGLVTLQRLRSARAAPLDPSALHLWYSFAEGSYGTAHERQSPDVLEAGEPGLYPITETEKRSDSTELLKQQQQRNEQQQVAGENGVGSAAHMVAEGASAATDATHGVEPCPLHKAGMTSSAAGALQEPTLPPDQQTRSADQGRQEQQHRPQPAPIATTAPDQHDSREPAAVPVCPSPQLLLPPPVTIPAVVPHICCAAAGAGAVTTGDGLNTSLPSGLTWPHKLLRNIQDVAGRLTHRASVPAGQDAVAVSSGAKEPQQMRLQVLLCHKANPDNRIMPAQQFRSLEVALRRFYETNVADEGTRNRGGEAGAASGIMNLDSGEFYVLGPELQPRITGMPDRHMLVYLPDEFPHAYCLQIQASSGSASDGGGQPVAASGDGERPAFGGVGEQLAGGAGGRMASFSNSGGSSYMSVLSAGNGGALSSTRRGDTAVAIGRKGHGAVQMCTTYGARYRFPCSSGAPCSVTADSGDNRESAAGDGQVELEVQIQLHSCAILGAAGLKEVGLVLAVRGSTADSGSRAAPTVCTTRTHGDGRAYNDVWGLPAIVALGVWLCSGLCQVASLPLSLCQADAGVALLQRLLNAGSLREALGLQLQPSGQWKDTAAGAKPLDSGDNGTAHMAATAVEAAITVTQLPEDELARQSTLEMETSMLQDTRMATRRDGQANVEAMVLEAGGAQRLVGNTDHEQAAMDTSSAAAAGLPEGISVPPMFSPLGAMDVEPIYIPASDGQLGSREGADASAIGTTVTGTTSYTETGVPALVAAFQPHAGGHDGPELTSSRHGDHGGYHGDGGAYAMGDVAVSAAVSPGDANPVVNEQEGASVQYALRDVDGSSGGLNHGVPTAGVGVGADADADADATVCTQGGGSMHDDGAGRVILEVEGNGEGLAGSCLTGFEQKPDAHMAVDSAAKAAAETAAAWEAARSVRKRALAMDPLLLRSLEEFGLLGDLVPVLEEMTTEQLSEAPVRLLGHLMAMSSMLSGTSRSGCSRTADATVTSTSTSASTSTTVPNSNVTRGAAGSNVCTARARISAVRVFALFAVSLYMARADMEAENGPPQIHATGLGSISPTPESCRSQPPMSAAVAAAAAAPLRDEQGTQAMSSEPELRHPRRLGSKYFSGALDRLLGLFWAARRVFRTAEFWEVVCRMHVLLKVGDTRGNPGGVRKLVETAITGVFTRAWPDDAVTAGIGDSDVPRLLACWDEILEAHRQLQKEREQDAAEHDDDEERNRGAAEAETPQGIDTAVRTQPPAPPQQPPLPPPPPPQQQQQQQQQQQQQQQQSTPQHLQQLSLQFLQMVQRQVVTQSHEQQQFLQQQRLPQQQQQQQQQRRRQQPPAAPAVPAVQQQAAAQQPQNQQHQAAHAQSQQHHVMGLLVEFAKQLAPLSLGNKDAVLRLFALGLVNGLGVAPGPAMASQPQQKVTVRLRSCTLAPSPTSGIRNYLISYIYQGLELTQTFSVQVPQNLYWEGLNRMVDQDMELPVGLVASAVATELRGRGMSLPEVVERALAEGYSRNVHLQFLISLLQYWRAQLRLQPGLQSALHTQLEPGSAGATNEANAGAQLAVPAAAVGVVAGGQATTIHELGQTLARAVNSRPRTEALGSQPSPLFSAAPVAGQGCPQVAPSAVTAVPVTTETAAEGALGAAASLGSQRQREAAASTQQLVQQGGMAGAAPRQRRIPTRGRLPKSSAGRTASTMVITAAVAPSQPPMRLVWPAPAPATPVAAPYSLAQVPKQPLPLSDSAVAAAPHVYRAQGGELHTAVVHALPVLHPPEQVQQQQQQQQQQPQQPQPQQQQQQQQQQQQQQQQQQLLQMRLRHAQQLALLARQQQLLKQQLSEQQRDQSQQRQQPIALDRLPTRGRRRLEEPQQRSAAQVTVVTRQVALQTPVGLPAIQYQLASSNTAISQPPPSTSAVKQPAMPTMPTSAPAAPQGQQVATLGFNAATQLPAHGQQTAGSVTIGNATTTVGVNMPANGVVMSAGVTTLGDIWNVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW75</accession>
    <name>A0ABQ5RW75_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_003871</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF41">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="506"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="563"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="61"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="680"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI61474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="723" mass="77727" checksum="E6CB674C9ECDF86F" modified="2025-10-08" version="1">MLQFLLGAATIVAPAQIGIPAGVTVAVLRAALGAAKRRRQRKANQLKCASTSKLEAKYEALLAEDVVEPVTLSWTNLHCGLPLKDGTTKSILRGVSGIARPGRLVGLMGPSGSGKTSLLTTLAGCVPSASKMELTGSLTVNGLPAAETGHRKAFVQQEDLFYSMLSVRETLSLAADLRLPQQLSVEVRNRYVEQLIGALGLTKAADTRVGDEKTRGLSGGEKKRLSIGCELVGSPSLIFCDEPTTGLDSFQAEKVMATLSSLARSGHTVVASVHQPRSSIFAMFDDLVLLSEGQPVYSGPAAEVLDHFARLGHVCPEHFNPAEFLADLISIDYTNKDVEAESRARLERLVAAWREVESCSTTGYDDRQPSSGLTVGTNDSEGAEVAVVMRRAALMPRSGPVRQLRLLLTRSWRQVVRDKATNVARAMSNLSSALVFGAIFFRMKRGQSSVQDRMGLLQVVAVSTAMSSLVKTLTIFPRERTIVARERARQAYGILPYLSAKLAAELPVGALFPALFGALVYPTCGLHPSLARFAKFLGILTLESFASQALGLAVSSVAPTTEAAVAIGPAVMLVWIVFGGYYVNAENVPSIFKWLPRASLIKQSFEALCINEFPGLVFEPDANGNGMRDGNQVLRWLSFDKSTITVRCGSQTRILLFYYWATFCVLRASQPRYLQVRPAPLQQTQQPQPQPQPQPPVATSQSANAEGAAVEEKTAGVLEAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWK7</accession>
    <name>A0ABQ5RWK7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Isovaleryl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.3.8.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_004567</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = 3-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:12276"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:57345"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.4" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01156">
        <property type="entry name" value="IVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034183">
        <property type="entry name" value="IVD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0274">FAD</keyword>
    <keyword evidence="7 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="16">
        <location>
            <begin position="46"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="15">
        <location>
            <begin position="160"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="14">
        <location>
            <begin position="269"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI61996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="429" mass="45560" checksum="DBB4D365055A241F" modified="2025-10-08" version="1">MALSMAFGFRANQSRACSLISSVYRQISSAAEASQSTDSGAGTQLRDFRAQVQQFAQQVIAPHAEAIDRQNDFPSSVNLWREMGTFGLLGITAPAEYGGLGLGYSEHCVAMEEISRASGAVGLSYGAHSNLCVNQIVRNATPAQKTKYLPKLITGEHVGALAMSEPGSGSDVVSMKCRAEAVGNGGSFVLNGNKMWCTNGPKADVLVVYAKTDPTAGPRGITAFLIEKGMAGFRTAQKLDKLGMRGSDTCELVFENCEVPSENVLGGVGRGVAVMMSGLDYERLVLAAGPCGLMAAALDVALPYSTQRKQFGQAIGEFQLIQAKLADMYTRLQSARAYVYSVAAAADAGTADRKDCASVILYAAEAATQSALDAIQILGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRYLIGRELFKESVEGRLLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWZ3</accession>
    <name>A0ABQ5RWZ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">asparaginase</fullName>
            <ecNumber evidence="1">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_004507</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08963">
        <property type="entry name" value="L-asparaginase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041725">
        <property type="entry name" value="L-asparaginase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00057">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="4">
        <location>
            <begin position="234"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="5">
        <location>
            <begin position="463"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="101"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="583" mass="62811" checksum="5BB49CBA617662A9" modified="2025-10-08" version="1">MLIVRQSTCQSSAHSTSNRRRDECAKHHNFPMRGMISNAVEPWLSRVACCSFNIGSGDVSANVRFLPSDGGGPNPPVTQSALTESDRHFQAQLNIVSQSFSGSNNEIPLDTDPNGHVSQQRTSVKLATSSLGNVRKGPSRRGRKAPSLPEHGAASSNATPRSSILQGNPVSVPQAPESKITKATPLVSDTAAGPSADVGPNQSIASGIAQGVYYRTPSHRPHSRLRTVTRPLPRVLFLHTGGTLGMDPEEAFEEDESHNLRVRAGATEGSYKGTLRPGEMLSSLLTMVPELEKFANVDLQVLFNKDSSRVSPADWITIARALDNNRPHYDAFVVVHGTDTLAYTAAALSLMLCGFKKPIILTGSQLPLRAPRTDARQNLLDSIQVATSAFSPPHVALQEVAVCFGGKLMRGNRTQKVNSAAYQAFDSLTYPYLATLGIDVDWNTRVLLRVEGAYKPRFKLDPRVIRIPVVPGSDPRIAYGDLYGRGVRGVVLEAFGVGNLPDQASYGWLPWLKDQTAKGLQVCLTSQCFAGPLQPSLYRAGQLASQMGVNAGPHMTPECASVKLMFCLKHPDLALGVPLAGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX96</accession>
    <name>A0ABQ5RX96_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="269" mass="29439" checksum="4B85D57CBE29F4A5" modified="2025-10-08" version="1" fragment="single">DRASRSEAPIFTTQPALERTFTTMLLLKRRFLQAFNVTNNKGWIPMHLVMPCGLRSTGSSPNTFKASALESGTSPNTPWKFGFQCNERYLAWDQSAQLQLLKLVLSERLGCTTTEVESRAEQLGRLLPDLLTRMECTRVDILEPLLADLPGLTAQLIGLRDCLPGVNLSQLVAKHPRLLQEFKDSAALAARLDELRAALPGINVPVLVAEEPHLLYCDIKLVLCNCTRIVPGCDPVKLLISQPQMVLSAVEAGLSSAMDVEGGAPVTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXA1</accession>
    <name>A0ABQ5RXA1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="29315" checksum="5EEBA7308D92FC3C" modified="2025-10-08" version="1">MDFVSSNSLFTFQPGSVWRLTSSRGDVLSDRDKHLGLYGLHNQYHVKVLGRLLGGSGRLLNKRPASSKRDNISDAWLQKHKFSRCQDPLGQEVYACTQCPFTGRTRSQLYGHMKRCRLSFPEDTNLNMDNENDFNTSDDHNEDHSSSSKDSSGHTSEVLSGDSSGHSQVQEKTALPSSLVSALIHVMDDLDPANPNHRQTLEYISAILDNLCQHSYPESEDDEPDAAELGVLSQLGAMDHGQLQLLEPTNPAELLASGSTVQAYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXA2</accession>
    <name>A0ABQ5RXA2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004871</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="409" mass="45661" checksum="134FE60508FABFDC" modified="2025-10-08" version="1">MYIQNRMSSSARATSYPGVCERTMLTRGGTISIQHRRGLTSCLKSQLTPHQTPRSGSSTTPLRTRASKISSGGFSPSGSEPEHEMQNPATFFPDTNWPAQKAPPKASLVPLNQAQHAGELTLNVVEFIHWLAFPLGFYVAHYIFVEAPVLATYLNGDTSRVFFLMLGLMCQVMGGICGIQMHMYEGWQVTPFRNLLELPPTAEAQVPPTPAEVDETRVTNYNNAWLRAVAYHILFTFQTLGLSFFSLGVFGYTPAMNALLGMNVAVALVWRRKPQLSEAEPKWLWRFFCERTVNGVTYPIMPLSVWLFVMFMVNTLVSLWAYKVFFGPDISALWPPSLLPWLAPLKSEWWAVVAPLIVAAGGFVEGYFAETSFDQRLHGLAVIILLMGVGLHYPLYWHMVQSANAMLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXC5</accession>
    <name>A0ABQ5RXC5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004476</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="11528" checksum="CCBA742A1EC18550" modified="2025-10-08" version="1" fragment="single">GGLSVTTPGQLDDLIIRAIFNRLFNFPFYGYFSESIPNQLTRPIELLPPPLRSRLDRTVVTPVGYDVLDRLSALLITPPAGEPGELIPAGCIGPVFSFGVRGGNCSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXM1</accession>
    <name>A0ABQ5RXM1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavin reductase like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="1">
        <location>
            <begin position="36"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="193" mass="21029" checksum="ABBB2FE722ABD92B" modified="2025-10-08" version="1">MLNGNRLAKCSPPGRGRQLVAKIRGAMDQSVPLYTKFSQPVYSLATQGTDAPTGPTLNLLTYACPVAIKPDRCFAIGLYLGTVSHDNMKAHRRGVLQVLRPSHATLFHLLGKTSARDVNKHSAIQEAGFCLRERYGVMTLEDAASVMDIEVVSDFIPCGDHDVVICRVVNFENFSEDANDALYTSGLRAAGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXW2</accession>
    <name>A0ABQ5RXW2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.960.10">
        <property type="entry name" value="VSR Endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAP" evidence="2">
        <location>
            <begin position="373"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="449" mass="46923" checksum="A7B494E72DF27CEB" modified="2025-10-08" version="1" fragment="single">LVSALWAMSSLGGPPYFQAETEAAVTILIRCLASAAVSAAPSSDSAATSAPSPPAGGGGGLTGWQAGQVLWALGNSRHATQRLPDLEASLLRSGGLPYMHPRDVTRVLWGFASLGYSPERLLLTIRPDWSWRGRTASESVHKGKGKGSKARGKDMKGSGGGSGLEVQGDVRSFSPQQLACAVWALAVMGKVDTGPFRSAWAQLLRRAANVPPAEPVMTQIWQANLAVHLESSSSSPTPKSSSLRASATSASPIPIDGTVTTASALSSRPGSRGTGGCGSKAGNAAAASSEDLTAAGLAAAAGAGGGLNVAAARSLLLRARDVFLTATSALRRKVQSGYQRQMANTLTGLRMMHLLEDNSAGYSIDITLPQLRIALEADGPTHASRTPSGAVLGATAMKRRHLQRMGWHVINVTYKEWDRLTSESARRAFLQERINEALLSRIQIDDNEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY68</accession>
    <name>A0ABQ5RY68_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="42101" checksum="04F4C0507D7373A7" modified="2025-10-08" version="1" fragment="single">MFCHKQARMTGSGAFGYRTKRHSLLAHLQICLGVVTKSASVDVVVILSKSAQASTRPQATSTGVDVCNSQMNSALSYPLSVTSASEARQWSLADALTNKSERLFGWRTDWPGPPTYAAAGVVPFAYREDEGQLYVLLSIQRSDRGRGASLVYTFLGGKVASVDRKDARRTAAREAHEETHRLLDKQMVLDVLLGTAPAYGSTRAAATAAAVVSRGSAAVAIAADSTNASSSNLTGAALPASDVALPATSTVATATAIAAAVANNYTTTAEVSVGNGAISPLLPPPWPFPLHSGQTTETHSSNTDGGGGINVNKSCDVQIDASGSDTGVGAGARAAETSGPSGCGRGGQYPVPSEYFWSGRYMAFAMHLPDSWQLAGGCAAKINAKQKHPEAEKAEGLAWITLRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY86</accession>
    <name>A0ABQ5RY86_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_005133</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TspO/BZRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15904">
        <property type="entry name" value="TSPO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.100">
        <property type="entry name" value="TspO/MBR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038330">
        <property type="entry name" value="TspO/MBR-related_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004307">
        <property type="entry name" value="TspO_MBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057">
        <property type="entry name" value="PERIPHERAL-TYPE BENZODIAZEPINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10057:SF0">
        <property type="entry name" value="TRANSLOCATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03073">
        <property type="entry name" value="TspO_MBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="137" mass="14940" checksum="681A7787AC230AF4" modified="2025-10-08" version="1" fragment="single">MMGTASWLVWKKKGKNTTALVLYGAQLVLNLVWNPLFFKTHKTDLALLDITALLGVATAATVFMSRAGNPAVQLPLMVPYLLWVSYATALNANIYLNNPSERLFTSRKQRDREIKAGKDAVKPGSMMAEAAKAAVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYM7</accession>
    <name>A0ABQ5RYM7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_004876</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1030">
        <property type="entry name" value="Preprotein translocase secy subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055330">
        <property type="entry name" value="SECE1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005807">
        <property type="entry name" value="SecE_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038379">
        <property type="entry name" value="SecE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00964">
        <property type="entry name" value="secE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37240">
        <property type="entry name" value="PREPROTEIN TRANSLOCASE SUBUNIT SECE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37240:SF1">
        <property type="entry name" value="PREPROTEIN TRANSLOCASE SUBUNIT SECE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="169" mass="18791" checksum="0CE0FFA6FED97BF8" modified="2025-10-08" version="1" fragment="single">SHRVIFLASEVALVLWIRFVTLLYFFVQSSTAMRCAVVQPFACSPKHKRVTLAHLCVRQERVARQLTRTCAGENEKPLTTQSSGEDGPLAPEEISRLASLRRQQQADLAKDSNVVQGAIEEAQLITWPSREKALLDTVLVLFIVAGSGAMIFGVNVLLAEASEWWYHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYP4</accession>
    <name>A0ABQ5RYP4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004997</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_bL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_bL36_sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052010">
        <property type="entry name" value="Ribosomal_LSU_bL36"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18804">
        <property type="entry name" value="RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18804:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="Ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="117" mass="13525" checksum="32B6E40E4643F265" modified="2025-10-08" version="1" fragment="single">MKVRGKVRLMCEFCKRIVIRLNRKQHHVYIYCTKNPRHKQRTKFLAMSTQAEPHCCNEHPGSWSTDVPVGVPCLPEPKYSLVQQHMLSSASRSLLANRTSSVLGELYWRAALEGRDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYT2</accession>
    <name>A0ABQ5RYT2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005477</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="110" mass="12364" checksum="5F22A396519FD6DF" modified="2025-10-08" version="1">MSEQLAVYRRCRRQYPGTAACVLLPAFCQRKAEASLKGMRRVHRFEPGTVLFNGVDREGMLAPWPGTSYSVDVWYEASDEGSVSVVPRFISRRCVGQCDATRRCADLMNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZS5</accession>
    <name>A0ABQ5RZS5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="518"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="851"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1020"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="389"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="542"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="568"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="852"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1042" mass="106422" checksum="DCEB2BDE2368B8FB" modified="2025-10-08" version="1">MGPLALAVDIDVLLSSGSIPEVASSSAEPPPSFSYGGGERPASATALPTTMSTGNLGKVTQRQTLTGSSFGTQHVWMRPGVRRFLLAIRPHFEPVLLVRRRCSTRRCCCICGRSGRSVSYSGGVIDSSPGGISAEGTTTGASCTDNRERNAGDCGAALLAAALPYIDPDGQFFGERVVFIQQDQPRPRTAHPRMTRTSCHPGGAQMTAAKSAVAAVAAAAAAAAAATPGPTDINSMQLFPVAAMMAVSDLSFPVAQISNFAALPAMLGGAPSEMLIVTVADRSLFGSGLANQVVQCQPYRIEYGKYDDVLDCLASVVSELLVEPSVSQGLLRLGLLHAKLQPSPMMQALHAQAALRQAVIAQQKQQQQQQEARAEDLGANVQANQSAHGQSKGLQQPLQQPQQPQKQKHAAAAAGVRKSSSTACAKSKKASRKAGSSGRRDSENSSGEDRGSGGAGGGRSASGIQATPSVSPLPPIPELSFLEVGSEWQDPAVAEGDDDEELTAAATATLMPAGVILAPEPFPCDQSSGGISGSSNCIAGTQDQAPGPSQQSPSMSSPPTLCCTTPDSVSTGSRPSRSSAGSNSSGNSNSNNNMASPAAAAASQSPHGARRMAWDDAPLSPSKGPGEGPGAANGKQALSKMSQNLSGGSSGSGVFSRGRAGSGSGGELGWRPSSFPAATKPHVTRHVSDRVSLPPESNKMAIGFGAVEAPPVSPLGAAANAVAAATSRLFSRILGGRRQRRCRPSDASGDGDGSVSAGVMAATAATVIITGAGRSATSATQTSSRTWGVAGEGSFHSNAVVQQVRSTSASAVEAPLFKTSFSGRTTAAAAAYRTTPHAISSEFVLEPGAGNGAANSVSASEATTSPVPSARTTPAPTTATATATATATADATISSPSLQSQMQMHSQWQLQMQSLAVAGNNYSIRWPAPSPTSRVMRGSLPGSALDSLGDFPLDLQEQPQQQERRTAPVYVLPPETVSAAMPGYKADTVEVPWGIVRDDFRIREQEEACEGLMRVLDATGRGDHGGGSMGTGGSHSGAVAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZY7</accession>
    <name>A0ABQ5RZY7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="253" mass="26879" checksum="8CCE907B3983E9F9" modified="2025-10-08" version="1" fragment="single">RRAADESVEALCPQQWPHRLVGLLRFPRLRRLDLGALGPDPAPLSRIAGLLKQLAEASSPPLTSTTTATRSPSQRLQVPAPGGGGGGGGGERGLMHQAPHPQRQVRDGESREGANVSACIPGEQLQHRQQRQGVPPALRPGPAGWGLEGVRLTLQHLTLSKECLVVAGRLMYDIIGSGVSGAQAVLTMPVGLGELTALRSLAIVRAAPAVSNAASSRAMSYSSGYHTWRPMLPEAWREAVAQARTRALNELEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0E2</accession>
    <name>A0ABQ5S0E2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_005511</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="590"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="649"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="896"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="934"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI62767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1106" mass="118864" checksum="F813ED8991AD46F1" modified="2025-10-08" version="1">MPLLSFVAWGRQLASNSTDAAITLLNCFRRMHGFSAMLSEISAEAAGASARRSIPLLRLPDSMECELEGVLAEHGLKPREARRLGLELAERLNSLSHTPQRGGFGVITTKPLLASGGRLRGAELLAAREAFEGGELLSMDDRAVNAALGLPPPLRDDQKHAPQMEKLVPKYKTEAEVMAYVVVRLAPEYAVCQRVLNEAVRLLEAAEVLPGAAAVKWRPREGLVYGAGVGAAVVAVLESCVSPSLARLVALEPSSLRQMMGLRVVQAHRAKLQMLRREKEQYVQQQQQQRNQMRHDDSLDGDMAMLHRSKRQQKQGVTGPQVSWVHALPPLTKASAAQRRRYDLVIAPYQLTVLPTVEERQRLVRELWDRCGDVMILIEPGTPRGFAAIAEARELVLGREGRKRLQLEAAAASGGQRQDGRAVDPRVVNKLRGTGAHVVAPCPHDGACPMLATKRHWCHFSQPLLLPRFTQQVMVLAGHKAQGRGRALNVQDERFSYVILRRGPRPAFRAAVSRDFITEGVQGDGTKEHGLAASVVPSRLPPRVVMNQIILNRAASPTTAARYPYESSIVTTAATAAAESDPRIGRRAGLADEAGEQPAARRMPLFGSQRPWGGDSSDVGIMKPSMETSVDAEMQPGGIAASLKQEAEVRGGTQSGQDGVARSVDGSVNNHSRSEDTDLDMNLKAMDAPRAAGSGGCGPISSTFSHKYPAFGLGLVGRLNVLQDAGLDWASLGQNGGKVDEGNEEVEGTMASIGVGDGSARSPGLDAAVAEISMLALGGVPKVATEDEFPASRTGMSVEALVAPAAVTPAMQGGIPTSDAVSLAPELQLRSNDLGATGPDGLVPELHVFGVHQQAALLTRDDVSDEEYDTTERQLVERIYGLPVAHIWQANRLNSGRARPVTPSEDDELIGPAEATNEQEAKGKQQQQQQQREETEEDEAEDEGEQLECKATPARAAAVRAAEASSYGWGRLVRSPRLRGGHIILDMCVGPQHHYPDSLNGTANGAPGVRTAPLAAAEVSAAEYGTSVIADGEVSTSDATSTPTRPEGRLVQQVVAKSARHSWMGAPAYRLARRLSWGDCWPDWYIRSHNTREMAPRTAPAATSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0L0</accession>
    <name>A0ABQ5S0L0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006326</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP186/NUP192/NUP205 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="C2 NT-type" evidence="6">
        <location>
            <begin position="7"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="223"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="275"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="556"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="927" mass="96016" checksum="E86B337CE9E296A3" modified="2025-10-08" version="1">MFSFGSKVKQKLTATRFEFSFVVHNLAPWPEGARAIAIGYQRGKRRRGATRSVYPSRQPGRLGAVVRFNERFDMPVTLYKASGGNASTTGLGPFKKKCLILAVLETDGRTQATAALGRVVVDLSEYAAVEHKETRTFMVSCNKAVHAAVGDPQLMVTIRCRWKKMREAGGGSGATLPDETASMSTDTTGSRMTANLSSFLRFKFTGKGNGPLNEEQDLRGFESAAPAGSATSGQVNGGRGASLAGDAPGLSKVMDTIQESHDEPAEPVPVYSSIAQPTSSRTATPHASVKALTPSSSVTGQDLSQRSPLRGSEMDSTREALEGGMMGLSLREIHRSRQPLAGTQATGGSAHSLAAAPDSELLAALRHELLTVAALEAAVYLARRPSGAGLGSAVTATANDLAVGCGPGAASGTVSFTGRVTLASASGRGAHSPARRLVRTVVAMGSSDGPQFGAAALRAVRAQVAAAAGDGLDTLSYWWSNIVHMRAMLACLPSSLGNVSASSASLAASQLPLGAGGEHWASAVLVPDLRRLERTLFGEVLVRMWDRVLLPTVLGSASRPGGSGATSRTSSPAVTKRAQQEAAIKKWFEGLEAVCSTLNRHVGSAGHAAMLRHQVLLQCLKRVDLLLFAHLLGGIEQPGVADLLADYDPHRTVAWMGGAGGGGDACTNGAFPYLDDASLPFARGVLTFGGGMSVKMTVTRLQQWAAGQAALGGAGGAGSTSAAAAAEAAAALASGADRSGGTSGGAAPGSALGPLFPLLRAAADLLMMPKELLLDRAVRLDVGAALSMRSVLHILERFKPDEFAQDSISPTILESLLEDAALPQMHLRSPAADVNSGYLPPTDSVLMRAVEAGSEPGLEYDADSEDEMEELAQVVAKSSIIAVSGSSGALHSAGSAFSAVRFRLLHDMWAASAPRRRGPVSVASETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1F1</accession>
    <name>A0ABQ5S1F1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GAG-pre-integrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAG-pre-integrase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="135" mass="15020" checksum="ED2A98E4C69EBF2A" modified="2025-10-08" version="1" fragment="single">FLASEAKSASLWHRRLGHLGLTNMKRLVQEGMVKGLEVTDKELREFEGRRCEPCILGKHARDPFPASEGKTQKALQLLHLDVCGPLPLSSCGYKYSVTMLDDYTGASLVQPIKEKSDVANFMERAITMLQVQSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2M2</accession>
    <name>A0ABQ5S2M2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="458"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="289"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="561" mass="60181" checksum="C20BE32A81AF0406" modified="2025-10-08" version="1">MVQEKRDGGPAPQGRRIPIPPVAAPSEKGHQAAPTKSGILSPRTRPTNGIGIGRGKDATSSAILSAQNFAKHRTPSAPAASKFGKSTSRPNSSRSVEDKDDIRQRDNSFRKDSLARDKASKDSIPRRQGSGAESANLTDAGKRLLLKNTAAPSPVTSPNGKAVPDPASNKALPRSFSSCRDRLNSVMGESEPRTNQVRRSMSVGKGRILANQTDQAQQQAANRARPPVARVNSACSTGAPYRQVAEKRDHSAGRLRPLSAVSGKSDMSFTSSCKSALTTITEAPARPAPQSSVSTGSTVPKPVGPPRPMLSLNLAALKSQAATSGAPVESCTSPCPEDQGPPVPKLDLGGILQRRAEEQASEPVFMVAASNDDIPEPPGSSAYVSQLDKRKSGSRQTSAKEEPAEHFYLYPKVNPQEKKRIEDICRVVPKKQQAAITDLFSKMVEARQNCEQMCFRGHRLLDQAQAEFKNRLAQKEEEAAQWRQEAEFLRTQLAMLLSARQQEENVGETSARLVKNQPEGGEEDRVAYLRSSVLSTFDSMAQLGTATSEAYGGDGQGDADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3B9</accession>
    <name>A0ABQ5S3B9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="129" mass="13666" checksum="79CF33E8B2034A92" modified="2025-10-08" version="1">MQASKFTNAVSALSSTQFSMLAGLLIMLAKQICGNASLVWHRHQMLLWQVVPTFRTSPHQFPVQNHLAPCAMDVAGMANAAATTGATAAGTSSGLPVPPHTGHEDEVMKMMILVPKLNISADVKLSDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3F9</accession>
    <name>A0ABQ5S3F9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_007662</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="10"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="329"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="568" mass="60088" checksum="44FD715D13E0E491" modified="2025-10-08" version="1">MQIQARKLLKVLSLIFAWYILSTCLSIYNKKTVGKKYGLFGNHPFPAPLLMSSVQFACQTGLAKIVFFMGLAQRTMARRMSWSDYFRLVVPNGITTGLDIGFSNTSLVFIDLSFYTMCKATVPVFLLFFAFVWGIEKPSLELISVVVVIVGGLALLVKGETHFDLLGFGLVMTASCLSGLRFTLTQVLLHGHHHSAALGGPLEVLEQLTPVMSITALMFSLAWEELWDVLPSSVYFNSTQHTVLTILAIMLGALIAFLMVWTEYQVIKETSALIFMIAGTVKEVVTVLAAVLFFGEKLTWINSIGLVIVMIGVLLFNLYKYRKLQATPPSPRKRVKQADGATYTAVETSDDNADGESKPSRRPYGGSSGGISTNGGITNNGSMSPPVSAAAVAVATASSVSSSPPGGCHGNHQTVPGLLSRTVAGLLTRGQGSGAAVAPTAIAVELSPLLPPGGASSINELSPGFRSVPMVLSTRDWDRERGPAERGTDVEGMVVGMGSSGGDPAAVIALSGAGISGPQRATRPGSRGLMADVSVEDAGEGAGGWVGPHQPLMAQAQLLPLASSPQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3I4</accession>
    <name>A0ABQ5S3I4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_007743</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052583">
        <property type="entry name" value="ATP-helicase/E3_Ub-Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048686">
        <property type="entry name" value="SHPRH_helical_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21325">
        <property type="entry name" value="SHPRH_helical-1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="1268"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="1861"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="579"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="998"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1093"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1144"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1722"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2033"/>
            <end position="2108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1044"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1724"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="2071"/>
            <end position="2080"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI64470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2276" mass="244474" checksum="E5A49BDB17FDA904" modified="2025-10-08" version="1">MPRKKGTPSRRLVLPLAGAREDDEPDCNEDLDDSAVPCGRPKSRRFEIELDPVPDSRPTKRRRRTLTLEPWKFDIHVDVIEVHKLLGFLQLNSVLAAVDEVGDAARAVVRVSGTGKPEEASSCLLECRYLASGQSLTAHLTSPTFETARNLVRLLQDGLLGFALESPCGPDAFCRSSQPVMYAWNSESPPIRLFQQTVRGALVTGISQDVRPSLSLDWESWQPAVYVAGPNFSALNWWTSDDVSKRTPNPSGTASSTAPPTTAGTSASGTSAVTTSLRLGVCAAALERLKASDPEDGHQHVWQAQLLDVLGWLLPHILVDGAKVKQPASPLEPPLPGVPLEAVPAPEGAGPPMLNGGAAVSMEQSLIRSPGKPAYSNGITGRTATLPTQIMSPGSAATAGQGFDPGWLYSAVKPAGGEPQWLDDPVELRPQLRPYQRRAVAWMLQRERAPKEEGGGVGTELDGAGGGLTAGRLQGRAMDRREAVAVGPPGFRSALLHPLWRRLYTLPGSAAEALYVNAYTGALASEPFPAPRPVRGGILADEMGLGKTVELLALITAHRFVPSLPEPPMEEKVAEAKAKAAGKGGRNIKRDKGGTASRQEGQRPPERVNCPCGVRADDPNDPSVEEYVGLWILCKCCNVWMHGACVGVKKALRDAWVCTRCLRDRALATVSEPCGATLIVVPSAILQQWYDEIRRHVHPGALQVVVYGGQMQPGISRMGHHLVGAFTRAGGNGGNAAAATAGRRRPVAGGSRGDEVDVAASMVVTASDLAAADVVLTTYDVLKRDVARQPDLEVPERSLRHGKRYEVVPTPLTRLRWWRVVLDEAQMVESSTAKATEMALKLDTVHRWCVTGTPISRGLEDVYGLLAFLDARPWSQRRWWNRCVQRPVEGGDPAGRQLLLSLLRPSAEPDIRGGGGGSSKGGLMWRNGKRDVEEELGLPPQSTHISHLRLNAVELHFYNRQHQDCAAKARAVLPPRVVAAMESGRLLGGADVQIEEDEQGGAGVRGDDSGEWELQPSRQDGIGRGSITREEVTAAVVEGSGPAHEQRQAEVQEEGKELPDTRLGRQEGGVELPDTLRELHDIQRRRWELWRQGGENEDRPGHARRHGGVGLSLGRPSTSAEEQLPHDDPMLVDCSEGRATVIEAAGGGGSGGEERRAPRGYSPPRLEGPRKAIMVLAASLDRPLAPHEARKLLGPLLKLRQACCHPQVGAGGIRALTAAGQPHHHHYQHHVGAPGGSHHNAPMTMTDILGVLVTRAKVEAEEAQRQLLAAFNGLSALMIIQGSIEDAIATYRKALRVMEDNKPDIDTDPLQRLHTLHNLDQMLATAAAACDAAALAAIPRTLRDHTLAEDAAAIRNRYLEQRRQKLASEEAAYKELISASLPFHGADGWYLAAIDLLVLEGHGQAAVDYIKEKLLEGDTYRQKTEVNASSLANRFSSLLGLKMLLNDSLNAIEEHRAEAMRLLDDLGTRTRQSQPAPEFVEQAGQCGRCRSGPTRTLVCEHCRLDERFIQWEVRLFALYSRALTAGATVTAEEAARRAQAALVRWAGHGGLHEKQGAEDQLEEGDDGTRRGANVAVANTSWRQSEAEMVLRLLVALLRQHFRHDADERHAEIMREGKAHVNRLEAQRRLLVAARSTSLAQRMLLYAHDELAMATMRMRLAREGEPISAHEVNLKLHPAAVPVKNVELSNDRIVAEGDLQRKLGSLRYLRKLQSIQKRQQERKQQVQQEYQQQQQQQNKHQQQPYDESATPSACADKPGGTDMYASAAVIVGSRPDSRGPTGSHSEGGSGIAAQEHSQVQMSKSAPGCPLAVQSAVPATPAPPYHTAVVDGPVATSTVAHPCDGDGDGGGGGSGGEGDEEFCPICHDPLECSADCVILPCGHQMHPGCSEALVAKTVAPSTPYHQKRVTCPTCRTRVHIADIAYIDPGLDSGSERATGGAGGSGGASGPWAGEAAVAVRGSFGTKIEAVVRRIKFVLEQDATAKVLVFSCWVDLLELVAAALATNAVPHVLARGRASLASALAAFKDHPWGGGELDLLDDEGPGGEGDRLGDSVVMPDMSGPDRVSNGDGGGSRHCDGMEDVREEAEEERGSTARNAPIRHGAASDAEGALQAPAAEVGMGPQPQLPSRGPVRLPLIRDPVKQILTKPRVLLLQMKQCGAGLNLTEAQHVVLVEPQLDPAAEVQAVGRVHRIGQGRPTHVHRFVVAHTVEEQVHKLAAARACRMDMAVAVPGARSGAGGVGGDGNEDLTVRDLAILLDTRWGVNTLPPTSSTAAVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3S0</accession>
    <name>A0ABQ5S3S0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007437</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF268">
        <property type="entry name" value="FAMILY PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047401115" description="EGF-like domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="106"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="802" mass="92003" checksum="4DC43698A7CD7114" modified="2025-10-08" version="1">MFRILLVLCFYTLIYVNSEAGVLGKTAGGRRQLQFGFKVPSIQYVIPEKVYLASRIPSDLMLESSQTEGHRRDMEGLRWRCAVARGTWCGQYRRQVSLIPRPAPRGSKECPNQCSGWGNCNYDSGMCECPAGRGGTDCGQDLKRPCTNRFRHPHEFSSPAVGHIGPDKHDLNPREPGWQASRCYGYCADDIALCICGYNSTFRHIPAPPDAPPWSPPLQWGRPMTDSCMIGHDKDGVVLDWGRPYIKYEDIYGPNGWCNSQDSDLKCGCAWDGNGWPCDGSRQYEPFCVNQCTGHGDCYMGFCRCHPGWYGSDCSRKYAGMEIDEGYLDRPWLQKTVVVPPASLPAPPVRVYPRPLIYVYDMPPVYHSRMLQYRIGADACMWRRFTESNGTYLLSMTYSVEVYLHEMMLQSDHRTYDPEEADFFYIPMYITCYMWPVLGWADFPWWHAPFAHSRPMHVANMILEAHEWLSTNFPWWNRRNGRDHIWLMAPDEGACYMPSVVYNTSIILTHWGRMDANHKSGSAYDQDNYDMAMPLEMFEGWKGFDWVNKTRPHPCYDPDKDLVIPAFKSPQHFHASPLLGTPPFERDILLYFRGDIGAGRRPQYSRGIRQKLFRLAQDGDWATKYKIFIGTGELIPGSYTEHLARSKFCLVAPGDGWSPRAEDAILHGCIPLVVMDHVHAVFESILSWESFSVRIREDDQALAAIPELLTGISPERVAKLQRNLARVWHRFTYATGPLLRQTYQDVLARNANVYPPALLNASYNLPPRVSPFRPNRDFPFEEDAFSTIIQWLYHKINETRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3S9</accession>
    <name>A0ABQ5S3S9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyanobacterial aminoacyl-tRNA synthetase CAAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007882</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025564">
        <property type="entry name" value="CAAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033344">
        <property type="entry name" value="CURT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33222">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33222:SF4">
        <property type="entry name" value="PROTEIN CURVATURE THYLAKOID 1A, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14159">
        <property type="entry name" value="CAAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Cyanobacterial aminoacyl-tRNA synthetase CAAD" evidence="3">
        <location>
            <begin position="64"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="151" mass="16164" checksum="8A543D42DFB575B2" modified="2025-10-08" version="1">MALVLRSATPLAAAKRRTLHAGRPAIRVQPRMVMTTAAAEPTSALVNENVQKAQAAAVEAWTWVKTKWDATENSEKPAVVAILAGVTVAQIAIGATIDVVDRIPVFSTLLQLVGLGVTGYYIFRLSADPAERDTIKSSFMDFLDKVTGDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3X0</accession>
    <name>A0ABQ5S3X0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007480</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF2">
        <property type="entry name" value="24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="398"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="611"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="730" mass="78911" checksum="F264A6CA81B484D8" modified="2025-10-08" version="1">MDLVDLITSKSAVLFPVTLWAVLLATAIYKLLWKRQDTTEAPLVPGLPLLGNALALGRHGVSYINKCRRKFGDCFTLSLASLKMTFLFEPSHIDYFFSAPDEKITFRPAVEQFTQRVFGLTSRLFFPLHSKMLMELRHLLVPGALAERMQSLGMRAMQLLPCYVHHAQVDLCSLCRGLVFHCAVEAMFGRHFVEQLPELLGLGHGKGEEEEEATVELNKRYVGEGNGEMATATGPEQQEELQGASVEVMGNAHVAPVWVNAWVGQRQAPPRPPVGVEKLARTFFAFEERFEMAASPVPHFFQTDFVRSRSELLRLFRLADGRGLFKGTPAGELLDRTTGLPLHLRPNLLLALLWASQANSIPAAFWSTAFLLLPENALHKASVLEELQLELQTGSLSPDGAEGAAAGAGSNEIGSSSAATSPPGSRRPGALWSQSQLVAAATRLAANRRSAISRCVAEALRLRVQSIDVRQVAAPLDLPSESRPGGRLRLPRGRLLAVCPFESHHDRKLWDAEDPSGAADPWVYDPTRPELRLGASSAVMNSGAGLAFGGGPYRCPGRYFAEQELGLVAQLLLWSYDMTLSYGDAANRFGAPAIPGAPAAAAAASSGGESVKAQASQVGPNEPTGANVPVSSGASADGADPHPQLRAVQGGSLLYAILTRLLEPSAMAWGFGWFDGLDGPIQEWRESGDPAGLLPPCDLRRLVGTKVPKKPCWVQLGRPLWVSNAQWTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S461</accession>
    <name>A0ABQ5S461_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Calcium-dependent protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_007595</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="27"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="328"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="364"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="400"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="436"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI64355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="54189" checksum="D3C7DF933100B2D2" modified="2025-10-08" version="1">MGDTRHSFSVANILGKGPHDPKLDDMYRVDKQIGKGAFGVVRAGSKKSTGEPVAVKSISKAKLVCKEDVKDVQAEVAIMNLVAGHPYVVTLKSTHEDKEFVHIAMELCAGGELFDSIVEAGNFTEKKAALIFRKMVEVVNHCHELGVMHRDLKPENFLLTSKGPEGELKLTDFGLGVFFKPGERFRDLVGSPYYVAPEVLRKNYSHEADMWSLGVILYILLSGLPPFWGDTEDQIFKMVLKGHIDFKTEPWPKISDAAKDCVRRLLDQDVTRRATAAQILKHEWLVKEGVALDIALDSVVLKRLKQFAQMNKLKKACLMVIGQHLTPDEIAGLKELFKSIDADSSGTITVEEMRKALAQWGHKIHEVELQQLMSIADVDGDGLIDYNEFVAATMHLSKLEKEELLQQAFKQIDKDGSGTISVVELEHELKKFGIYDDAKELLATADTNGDGHIDYLEFCSMLRNNNEALKQSTRAIKRQYSKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S522</accession>
    <name>A0ABQ5S522_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037734">
        <property type="entry name" value="Thylakoid_lumenal_17.9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36783">
        <property type="entry name" value="THYLAKOID LUMENAL 17.9 KDA PROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36783:SF2">
        <property type="entry name" value="THYLAKOID LUMENAL 17.9 KDA PROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="250" mass="28203" checksum="93A7BBE8AB4E6AC3" modified="2025-10-08" version="1">MFTFPFKPALRYRARPASDCKRTASRSALTRNEADLTCESNKHYKNYGESPSASRPPWALMACAISCSVAMSLTPQCLANPYLESKKTIILGVTSEGTIRGCLGNVNPNCVSTASTNELYAPAWRAKTKTARDAAQEMEITVLALYPEWSLAQSQTYDFGEYRAFLVPSLFGKDILEFLIKDESVNNRNWEEDREGPYVTYRSLAGSVKYIWPIQQPLSDFGAQKARLQELREKLGWQVIGCELIECYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5A9</accession>
    <name>A0ABQ5S5A9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_008488</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="55"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="1435"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="441"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="996"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1164"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1517"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1612"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1645"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1755"/>
            <end position="1812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1888"/>
            <end position="1944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="489"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="502"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="527"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="588"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="617"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="681"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="705"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="729"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="764"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="783"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="801"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1008"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1043"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1092"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1122"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1182"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1229"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1296"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1314"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1570"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1618"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1645"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1663"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1683"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1694"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1783"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1801"/>
            <end position="1811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI65065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1944" mass="195165" checksum="A097C9E81EB85FAA" modified="2025-10-08" version="1">MASFELKTLAAQNVGPAVIRDVLVSPLGVVILLTTGASLLVLDCRNGQLQLRHRIQGQQAGITAVALSGKGSELVAGGVDGFLKWWHVLSGDESCSTQFPIVHRGRTESISNGAPVLAITAVASVKGTNLVAAASGRSICVFGPGGEHLHTIPGSTEQILSLSWATPDLLVAAAGPVLNTWQVNQSVFIPADTYTPTDAVDMSIVAASPEGNRVAAACDNRTVVVWSLKPRPSANGANGTRRTCDTLPHQPDRTLVEGLEAEVSSLSWESHGRFLAAIVGGRGLIWDMSVASPMVGTAGSCGYQPCEMQISALQDGTLPQLRWCKFQPGSSLLAAGDDQGILHIFDTSFLRSAAGSSVAVLLGACRSAPPAPQNLVGSIDAADTVASNAADPDVYSGAGVPVPAPVLVGWNGSQLVLASCATGDVALLELVRHSGARPQGNAVQQQLVQPHRPGPVVRRSAGAEPPPVTAVTASPDSSTGGAPDEETEGDARLDDSSRSDCSRLSPSPCDSSSTLVRVPLPPPSTSPPSSKEQVPVQVQPGQPAAQGQQALGPGTTGAGGGGDRGPRNRRSTAVGGQPLTRGGGGSYNRLNDSGSGSMPFQAFSGGSFDNPADTERAAAAAAAAAAAAAAAAASSTALSTAPLGGGSGSGTPSRGRWQRDNGGTTGAQGISGRNFSEMYSGRGGGTAGRGAGPRGMPPAYVDGETQGTFWQQNHAGSAAATTSFRRNSSSEAAATEAEGGTAPADTAFTAAAAAAAAAAASSITPPPPPPPPFQGQRRSGSWQQPQLQQQPQQQRGESEAGSISHTGRRSGSSPAIQPHDRPGVPHVAAVAATPIMPLIAAASPAGPSGVPGAVSSGSALVPVEASGPYAQMQGHPAWAWGAVPVWQAPPGTAATLPYQATGPATPGSLPPGPVSAAAVPAPGYMAVPVMLGPGQAVAAAESTQYGMQYVVYQQGMPYVPWGYGYYPYNTGVIPYGSGVVGLTPPGSIDGAVGSMANSTGVPDRSGGSAASSISNLARRNRGASVGDVADSTPPGTFVAGTALQGSGSYGSSPMSARAPRRGSYDAQQQPPSGVGASLVGPAATSLGRRSQADSSLQQETGTASHLPRQGSMGGISSKELPRQQQPQQQQPRSQAVAVTIQNTAAAASAASPVPGGVATAVATSRQSMGAPPVPNVSPSSSLERKSAVQEHTSEEEQGLSSVGASATGAVRPPSSPPAAGQAKPDSRETAAAAAAIAAATAAAAAATAPSTASVVLSSAAGSDSTPRDLVDRGALPASNTKEPPAGPTVEGKQPRPMQAQSSLQQSASSSSLSVPPPPPAPSPEPAETPELPSSTIVEAVAAEPAGASAAAAAEGRQPRVPPPVVITAGNNGSNAGQVRDATFTKRPVMVSPPVSPFLAPAHSSAGPAVGAMIPGPSPGAAGTAGGATDAEDDPRVVYVGHLPQNTDEHALYFAFSKFGHVLHIQIMRDKETGVCRGYGFVTFESAQHASSAMRGLNGQPLPGLPFSVDRRPLRVAPAVKSQDRRPSIVSLQSNGSSGLLPRQGSSGFFPRHPQQQQQHPLILSATRYCSQSGPQQSQQQQTHAVGQSAVGEGGTGVAPALTGGSTASIATAAAAGGPVPPPPPPVAAPVIPQPMAQQPLLPTLHHTQQNSGGTLQQGRPTVSGSPSQSRAGSSGTPGTGLFGSRHQQQQRGSQGYGGGGGGGSPYQAFGGVPSHQHHVQAAAVLQGAPPHGYPPVAVAPGMPVAVAVAGGASGGYGSSIQPPPPPLNRQRSTGAQQTHHHQQHEVSSHENHSAEAGQNPQSQSQVQQSSQPHMVVYYGQAPGANPYFYGPHQNQYGMYGVTHQAAAAAATLAQSSPGSYYFAAAPMPYMAYGGQDVAAPPPVVYTRHASQHATQQHSHGHGDASAPVAANPGGTGSTRHDGMAGIKQQGPVSARGEAGTGSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5D0</accession>
    <name>A0ABQ5S5D0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein SirB1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008538</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032698">
        <property type="entry name" value="SirB1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31350:SF21">
        <property type="entry name" value="F-BOX ONLY PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31350">
        <property type="entry name" value="SI:DKEY-261L7.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13369">
        <property type="entry name" value="Transglut_core2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein SirB1 N-terminal" evidence="2">
        <location>
            <begin position="213"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="555"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="320"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="428"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="660" mass="70643" checksum="40946A736737440E" modified="2025-10-08" version="1">MQTGYFRGHKSRHSYFKLNGNVVILTCTPGPRRQGKCSAKQLRLPSPCIPDVQLLKAVVTCAAGGVPGSSDEASPDYSDNRLPGQESGMDSSVGGSASADEPAGTEASGPALTLGSEVLERMAKLRALDQYRRLMVEVSRITVLQQQQQQQQQQQQQQQQQKDMNKGKQAPAPGAAVGDASAAFANGDNSSLLLRGALLIARHRYPSLDEGKVYGMLDDIVRRVTALLPLDPAERYPLRVVGAINRVLYTDYGFRGNEEDYYNADNSCINRVMERRVGIPITLSLIYSEVARRVGLPMRGLNLPGHFMLQPLRPPSRNHTASAASTASTASAASTASTASAASAASSSESDRGTSDESSGGASTVGREEGQEQQQQQQQQKPRSDSGNLASDFGTQISQAAAGCGDSSGGNAAAAAVAAKATAAEKGMETAATEAAEQGGSAKEMADGDPPSGKEAKQEEEEGEEEAGAPLEVLVDAFRLGEVCLPGDAEERISAVLGSPVRIDPRVLKESQPLHPRMFLLRLLSNLRSIYLSTQQVELLLDVVKFMRVTLQTERPMEEEEGDGEGVEGRFPSSSAPTLNPLRSSFFGGSIPLVAASASLARDEGLCYFALRRYPECLEALREYLRLAEQSGNGPLPAEEAKTIVVVMEEARRRMTQQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6T2</accession>
    <name>A0ABQ5S6T2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_009197</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045907397" description="GOLD domain-containing protein" evidence="10">
        <location>
            <begin position="26"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="34"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="130"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI65627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="211" mass="23958" checksum="99086533327B61CC" modified="2025-10-08" version="1">MKIAGCAISALLACLLATLGIFTHALEFEMQSQLKCIYEEINNNVIVVGEYKSLSKELNHPSPAVTVKVEDPHGNVLHEATGKTEGQFAFTSKMAGEYKACFSVPDIQTAYQTKLKLDWRTGVAATDWNAIAKKEHLDALTVELRKLEDNIREVYNEMLQLQQREQEMRNISEETNTRVAWFSIASLGVCVASTLLQLWYLRRFFKRKKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S708</accession>
    <name>A0ABQ5S708_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008574</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14596">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14596:SF72">
        <property type="entry name" value="ZINC FINGER PROTEIN MSN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1234"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1520"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1564"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
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            <begin position="1595"/>
            <end position="1615"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
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            <begin position="2885"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
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            <begin position="2915"/>
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        </location>
    </feature>
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        <location>
            <begin position="2947"/>
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        </location>
    </feature>
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        <location>
            <begin position="664"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="754"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="849"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="979"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1322"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="1482"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1690"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1762"/>
            <end position="1951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1982"/>
            <end position="2055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2142"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2389"/>
            <end position="2424"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2440"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2553"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2649"/>
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        <location>
            <begin position="2719"/>
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        <location>
            <begin position="3124"/>
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            <begin position="671"/>
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        <location>
            <begin position="754"/>
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            <begin position="875"/>
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            <begin position="2146"/>
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            <begin position="2171"/>
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            <begin position="2475"/>
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            <begin position="2570"/>
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            <begin position="2601"/>
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            <begin position="2650"/>
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            <begin position="3124"/>
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            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
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    <sequence length="3144" mass="339571" checksum="FE6522F2E678D414" modified="2025-10-08" version="1">MTLARLPPGHLITQRPQQHNLRDRSIRCRPLNPLPNLVPLFFWCCWCFAALLPLLHSSSLAQTPPPAQQQRPATSSYGPWNPLEDLNEQHKAVLYFILSGNTTLWSRPEVATRIGFGTAPWRCINQCRTIYFSSPAECWPDCTDQEACPKGGVVNYDYKTPCCQLNLRQQSHEFTSFKFNTTFCNLYSSWEQGVRPSVCDFNVYAARGILPPLGAEDPFLAVSCTRVNNTPTFHISGRLYRRDTPRRILFDSQIKRIDVPRNIVSRISIRHTVAWHHPSINISEPPQFMMIHELSCLALEEIYLEDVFLVASFLVNAVLPDRRGPNGTVFNLWNHTTWNCNLKDGKLYNMTDKGFPGRMGRNNRTSYFSMMLDPCGVGRAMSQSVRSSLNPDIAFRNTAMSNCFFSPMTELNAMRWTGRALFTIPSQPIVQAQSFIAYGNQTYIKGWRSTADPLAARAFLPDTIKRITIKRTNDGSHPSASWKGRAAIVGVLPPQWAYLKNLEHLDLSDDMGQGQLEGSIPSTWLMLSKLRTINLTGHPKFCKDWHRLVSYQIQEFVLQTPYGPTWGLRRRYYGSIHPQNRAAQWNITIFDMDGVGWQWFDRSTGEAGYTNIIAPDSRCCWDTFSEEFAARNYTITGPSGTDGPGDYAGLRYERDDYCERAAPFIPTAAGPRPPIPPVAPKPPDAPNAPPFPSGQDMNATTSISLRPPMFPTLPQIAPLLPQMPPQPRLPPNFPGKWSGAWAWRPILPPRPPPPLPPSPYPSPPNPPPSPPSPAPPSSPLQVDCSLPATQASFAIPSIARGPFYIGISVLGWSSSTYCKVCGCQISYASLARGVSSLYASSLLSQPQVQGEEANSAHVTSTDSRNSPRHGAVGSSVAQGSTQQQVTVLMAGKQQNDDSNSSEYQLTRPTEYQRENSKLAHLLSSGVDSPRRQRRVVATDFDQHLPSLEDLITMGGKQPTNSSSTGGGGVLQVIEINAALPPSPPQQQQTEANASSNGESTAERREDRPAAAWIMRPQYNGDYLFRIQVGSTVWWRWVVVDIEPPRLSGQLLVSKSSVLIADSLTSSKQPALRYLILQINMSEPVQRFDMAAALQVGGGTTLFSADCFESASTAAELVKRAPTMVVATEGGSTSTMAGGSGGGGPTTSAPSGGYIDIGVGDIDYAAAAGVPYVRSCLAVLYVFEGSKPRIILPEGAVTDLAEIPTSKSLVLEAELSAKTLGPSSVADLTGPAGRFVSTLLAGATAASASASTLSIFSSRMSMLQSAYHIQVLAMTASFASPGVSSRYRQVAGNLRWSVMGLKGNIPVLDRAFGDPDNVTIPDPARSIGIDMNTWSKNSSDTLTTTGRTPAAGNGSTLAGSQAAPPEPISRRYQRSLITSMSSVVVSPLAAALGVPPPSALPSPPPSPQQTNSSTKSSSDLATSQDLLILWLRSLSEFMKANGSSSNNSSSSSVTNSYIIGGAAAGNRTAVAVDASGQLTTSNLSDGGGKGFAPSPPKSSRGGAAAVSTPTMTTVQIDNQDLMYTLTTAAILLTIVLIGHTVMVWLYRAYVDPNLPTALQFPRLELTFTGPLIVAITFFSCNTLGAPPASQRRSSKVMAILVLVFLVVPYVLLLWWLTVCRWYLEEEEESDDVVDGGKSVVVDRTLLPHRQGPPDSAILLPHLADNAAALAVPNDSGDNMIGPHWALVPVDNNGGFQKDTKGESQGTEAAAEDNACGNPTSRQQQGRNGSYAFVGRSKEFLSSRLPPDYSNKPTVSLGEKVIDKGSGRESSWGPAAGGMLGILGDGQQASSTTKSPHAQSGLLRPRHRPLSSTLQVSETAKTLSSQMAADAQQPQGAADERISALSHEPSAVQDPRQDGLGDVSSTSQDNPQTSINVPPPPPPPLPPPHHRHHHQQQQCQQQQQQQQQQQQQDLRATRHQPGKPGAAVVVSDVRDRHQSNDTSEERQAEKADGVVQASAGYCIGYYTDGPLNIKPPLLFPPITNRHASSSSNTAAARPGSSSRGNVPRLAPLEKQSRLRLDSSSSSRASSGRPTRTLTNNNSTWGDSENGLLLHDEDHNPPAVLAAAAAGDSTTGVPSLMRSLGLKIIGNIIESHDGPVVLSDSKPNTSNMYMPANAAAVAFLLPSAALDNKNNGSSLTITQLQDSTIAEDEDEAADEDSRVQDAGVNPALVPTNSVLEQPDVSTSSNNKHREETSITVYPIAKDVTVASNGKETSGIEVSNNQQQRIQTPTIQDLEQLLHPMARSRWSTKASSSLAPRASQLLSSPIIRQLRVLRPSVLSKVRDDNNITSSMAIVGDIQSQGMFHHHEQTGSAAVGCGARHLAAESPGREFYVPPEQTIQQQRPRSRPLSSLNAASITKMATDYKRASEAGNDGDRSIKRLFPKFSFVSSSHVKPAAATTDVDAGVRRGTTPSNPSRPGSPYQVKAWDDAATTTTTRTATAATAVTQATPSVGRPLPPSVIDMSDDGPARQKAESHPPSSNSRSSSPRANLDIASAATRTWSPRQQKTDLLLSRLPDEAELLMGGGVGSGSSAYDDSTTTYGVRIAARAPLAEATEITDGERPTVNYVNTEEARPTTSESKDNSKKRGVKVSLPPIAPRPATPNQRHTSNQGRNITGTPQDQLALFNVISLPDQHTPSLKTVTSTASGLVQHAAAAQQQQQQPHQQDPKPQQVNIVSRRPTPFDRYMLSTHNSTIAKQHDTADALLSNNITYGSAAGNLLGNHDDGSAVDTSRALSDDEGAPRTAPAVATPAPTAVILPQIESRAQKTSMARRRQTQIMFKLGEDAFVPSAPPSVDERLWLLYPGKHVPMYMMRYRPGCGPLQLHITPSVHFLARFECLFEEAIGEGLGQIGRETKMILASAAVNVTHKIWCSMAFGVLSHCSRSVAQLVLLWVPQICIVVYLVMFRPFIEWQRQVMELLCHVLEMVLFTCALALVNSTPDNAASTTYLMIVCFLVTSLTVVAYQIWHMVHIIRDIWVKFQKLLETKGMLTWGRFLQRCLSGNDEESQQDRRARLEAKQLGDGAVAAVKEQQCTGPNDDGSNNIDALPSGTPNHHQQLSNQQQSSATSQQNSVQIAKGSGGGATRNPSLSQKMEHGVHDSNGGGSCITIPESQEMVREENVRVTYENNVVQQDTAAGGSGEDKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S789</accession>
    <name>A0ABQ5S789_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="267" mass="29228" checksum="4DF20820D08AAF0E" modified="2025-10-08" version="1">MTAGYGVECGSFPHSFASYSDTMMQGVERPGHCSWNFIPRAGDMDTCPNSVLAAGPRAQPGYLALVSMPTVAEDRVEDRERDGEFECSNTEDTELRRLPPALRSGTLPQLHYPSHPQGNRQQQQQQQANRGVLQHLEHLEQQQQPAVRHCSASESGWDSELLGFAVVSGSGSHPGARIYNGNAVDGEGASHRALHQLQLEHGPEQQGRLETLQGSTMVDWLDLGGRLTHGPTVTHGSTWQGTASTSMTRVRLARQRSVGMTRKRVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7V7</accession>
    <name>A0ABQ5S7V7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_009496</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04842">
        <property type="entry name" value="Peptidases_S8_Kp43_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051048">
        <property type="entry name" value="Peptidase_S8/S53_subtilisin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034058">
        <property type="entry name" value="TagA/B/C/D_pept_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399:SF4">
        <property type="entry name" value="CELL WALL-ASSOCIATED PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399">
        <property type="entry name" value="SUBTILISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="2409"/>
            <end position="2434"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="448"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1272"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1445"/>
            <end position="1815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2006"/>
            <end position="2027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2185"/>
            <end position="2382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1465"/>
            <end position="1815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="2217"/>
            <end position="2234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="2241"/>
            <end position="2250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="2258"/>
            <end position="2299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="2309"/>
            <end position="2341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2345"/>
            <end position="2382"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="519"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI65864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2447" mass="259829" checksum="542B4436FF91E346" modified="2025-10-08" version="1">MTYMFISCVNSQMKRNTFYWHRKRGDGMLLITWLVLANAANSVLCDGVVHLRSRKVELRRVSTAPSSFQVLFDSGQQESEGQQSSVVASTNPMVYVHDQGDLGKNGKDGNDPAAASALPAAVVASSGPVVGEEPNLYFAQYREGIGESLRDDLASQGCSVVSYIPNNTLLVYGPYSFVLRAAARRDAFMGEYDFDLKYTSDLDRLKSIFSSAKRRRQRQLGIVNDGSPTTRGFEANDDVAANGGDASGGSGGGGGNGATGATEVQHPILGHIRTWTVYDRRRSSNSSSGGSNSAERALLAGSNTTVGASSTNSTAAAISLYGVNVYIVPIFQPEAVRYLVLKSWVPQLVNMLGRSAETKDPCRPRTLDKALYDSFAPRLEAYFCAEDFEKAITWLSKKPQVTWLELLLKMRTSNAAGGWLSQTGDLDTARYYNLTGDLRPYWKAGVMGNGTLVGVSDTGIDMSHCLFVDDNFDPASLQSQLRGSPLKWTLPSHRKVVQYAITNLASNPSDFYGDMMGGHGTHVCGTVAGARGLSDSALPYIDTGAAPAAKISFLDLAYPYSSDLYVPDIETVLLPTHYTAGARITSDSWGSVGATGVRYDATSMGYDLFAWRNPQVLSVVAAGNSGSSGFMATIGSPGTAKNILSVGASTNHPKNVTAFGDQSISLFLYEDGNGITQQSAFWPEDGEGTYTNRWRLLLQNKEVPVILANPIGACSPLVGNYTAKVVVVDFGSSSCADYMRALNTAYSKAVAAIYIRSDDEFADEAFSTSIAFDSYTIMFSLVTKGHGQYLKAVMQNSANSNFHLTYLSYPAVNIGVDSVAYFSSYGPLPDGRIKPEIVAPGSWVTSASAGNGVTAGANSQSCSSMTATYQGTSMAAPMVAGHLALLRQYFKDGFYPDGDRNLMTATAFEPSGMLVKATTIAGAKSLKGGFTRAAGIILDNPPDGYQGWGRLDLSGCLPLPRLTSPNMRIQVADMGQIAQGETVYLEGIKATGTGPIVAALVWHDYPAAPYASTVLMNDLDFKYSINQGPLLQTREDHVNNVERIELSSLTSGDSVTLVVSGTKIVHGLMGTGSDAALPQRWAVAVVGHFRGTLRTQLNPGYARPQRFQPFIDNGSTVWDYEVRQLSGSPAGCVAIEPGSIVPAWTSNCGRAFTKFVIFEEADNSTGGYVYVVKDYLGRCLTLAADRKNMTFSTCGSDMDQRLALFRNTAYTEKTALKLVPRPLLGTAGSDRECLSQVISPVSGSSGLQTATCSENDIRQTFELQPFLITAPPLPPWPPSSPQKPSSPPLPPPPPPSPSPPSPPVMPHLRFQASWALGTEPNIDDLDLLVAWNSGGAEYLITFGSTNIRGGVYEGDNTGFNPRRNWEAVSWSTIPLPPDNATYRICLAIYQNKGYAYNVTLTAAVGGFVVKTVLKSIGPILNSNRRTCFRSTPTYIDSFTYPPVLASSPPQTPSPSPSSLLTPSLPLSPSPLPPSPLPPSPLPPSPLPPSPLSPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLSPSPLSPPPRPPSPRPPSPLPPSPLPPSPLPPPPLPPSPHPSPLPPSPRPSSPLPPSPLPPSPRPPPPRPPSPRPPSPRPPSPLPPSPRPPSPRPPSPRPPSPRPPSPRPPSPRPPSPRPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPPSPLPSSPLPPSPLPPSLTPPPLLPLRPPPRTPSSKPPSPLPPHPLPLAPVLPSTLPPVRSPPYLPAPVPSQSLLPALPPIPRPPSPLSLPSLSPPDVPPPWLRFVASWSVAGVPSTDNLDLMVSWTFDGTTYSITKDSQRLRGGVYEGDNTARDPYRSWEAIVWNDSALAPDKTRFRACLKWFRGGPSYNVSLKVDVRGKNMLSTSALCDTSLFLVENRIISCNISSPGFLGSFVYPPSIPPSPGPPMLNPPQPPRKPPLFPPSPLSPPSPTLRLPWLRFKIEWFARPQPSNNNLDIVVTWISPNGVEKSIGSFSPSVEGGRYEGDNFPPFTAGTNFEAVSWNDSAAPPSPALYHVCARWNSTGPAYDINLTVVVAGDAIFNYSRKWETAQPSTSVCTNNSRGFMRSINYPIRPSIAALSPPMPSVWPQSKTTSSPFFPSPPPPTLQRQAILPKPTSPPPTPPQPPRPPLPPSSALKPVRPPPRPLSPPKSSSAPPTSPARRPMPPLPPPPLRPAPLSSPPSPPLHPPTPTASPSRPPRAQSSLSNPPLPLPTPPAPTLHPKPPFPHPLTPLWPLPPPLAASVVSSRSSTTKEATSTDTVATGVSLSSGDSVRQTGASTSQLSGSNLVDAVAYPSNFPTAEDDGHTFPVAAVTAGGASAVLVVVSVIAVLVIRQAHLKRLRVVPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7Z2</accession>
    <name>A0ABQ5S7Z2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL29m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_009400</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038340">
        <property type="entry name" value="MRP-L47_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010729">
        <property type="entry name" value="Ribosomal_uL29_mit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21183:SF18">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21183">
        <property type="entry name" value="RIBOSOMAL PROTEIN L47, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06984">
        <property type="entry name" value="MRP-L47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI65784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="161" mass="18945" checksum="081E7667DEDB3408" modified="2025-10-08" version="1">MQRLLTAARQGNAILRTQLASARFDIQRPICTSVCRADLREFFDWESRDGRGNEAYGRAWNVDELRAKSWQDLHKLWYVCIKERNLLLTEMGVKNIPKNAAEQRARGIPRGSEMVEQDQHRLRYREVQTTLRNIRQVLQERVDVQLVPALKQEMQEVINAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8U2</accession>
    <name>A0ABQ5S8U2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009494</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051048">
        <property type="entry name" value="Peptidase_S8/S53_subtilisin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399:SF4">
        <property type="entry name" value="CELL WALL-ASSOCIATED PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43399">
        <property type="entry name" value="SUBTILISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="7">
        <location>
            <begin position="374"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="147"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="187"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="513" mass="55136" checksum="45BD223D4C6485D9" modified="2025-10-08" version="1">MIVGDAKALRSVLSSGNCFVAAYIPNNTLLVYGKYSIVLGAAKRYGALMGEYIADYKRAPELNVITAAMSAITERRRRRRRRLGRVYEPTGTDAGADGIAAEASAATGDGRGSAGGSDIGRDGRKEVSEQRQRLFTILRGLETWSWRSGGGSSSSSSSSSRSSSTAKSKSSGAQNPGRRQGWVSARFTRTASTKGDAASSSARLHDAVPVVDRRLLAAEGSSEEEEEEEAPPQLYGINVYIVPGLPVDMVRSIILDGWMTDLSKALQQLRNADVNDDDDDGDYWEESCSPKAIDAALYESFSPRLQAYFCAQDIEDAVDWLSRQSLVTWLEPVLKATLLNAAGGWLSQTGALDITRYNNLLGDLRPFWKAGIMGNGSIIGVTDSGLDMSHCMFMDDNYSPAALQNMLTGSPLRWFLPNHRKVVQYAISHAAIDPEPFYGDEVGHGTHVCGTVAGARGLSSKSLLYTDTGSAPAAKLSVIDVKSPNSTGQLVSPELVESSILPSLLCRGADQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9E3</accession>
    <name>A0ABQ5S9E3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">biotin carboxylase</fullName>
            <ecNumber evidence="2">6.3.4.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">VaNZ11_010443</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.14" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF3">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0092">Biotin</keyword>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="17">
        <location>
            <begin position="91"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="247"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="15">
        <location>
            <begin position="718"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="GLI66558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1136" mass="119386" checksum="BA8E11E679F6E903" modified="2025-10-08" version="1" fragment="single">MTGDGRAEEGGGTAQLATRRSGGGTHVTAAGSFGDGPQVGTSGPTATLLSYSLLQGQAASAPRRGAGAPRRIPSLSKQLEEYVTELGGDRPIYSVLVANNGLAAVKFMRSVRSWASQALGAARAVSLVALATPDDMRVNAEHIAMADQFVEVPGGTNNNNYANVELILQIAQRAGVDAVWPGWGHASENPEMPASLAAHGIRFLGPPAGPMAALGDKIGSTLLAQAAGVPTLPWSGSHVAVSVEECKGGVIPPAIYSAACIPVDDLEAAVAACREVGYPVMLKASWGGGGKGIRKVSSDEEVRSVFKQVAGEVPGSPIFAMKLAPASRHLEVQLLCDIYGNVVSLFSRDCSVQRRHQKIVEEGPVTAAPQHVLRDMERCARALARLVGYVGAATVEYLYCIDEQKYYFLELNPRLQVEHPVTEGITGVNLPACQLLIGMGVPLTRIPYIRTLYGKDPKSAEPFDPETTPQRPPEGHVVAVRVTAEDAADGFKPTAGRIDELHFRPTPDVWGYFSVKSGGGIHQYSDSQFGHLFARGETREAALRAMAAALRDGVVVRGEIRTTTDYVLDLLASPEVTNNSIHTGWLDSRIAARVKPGRPPWHISVIAGAVVKSAAAVAAASSEYLGYLAKGQLPPPGISLTRMEHTMVIEGFKYAVTLVRRGTGRAAVALNGSSVEVAYRKMGDGGFLMQVDGESHVVHYEDEAAGTRLLINGLTVLLAAEVDPSRLTASSPGKLMRRLVPSGGRVERDQPYGEMEVMKMVMPLLSPASGVIRWVVPEGGALNAGELIASLELDEGAEVAAPEPYPGTFPELGPPQILSNRVDAVLASAVESARSILAGYVMPYEQVVEDLVTCLDSPALALLQWSAEFAVVRGRMPAALVAALESAVAAHAAEVHAAEAAAEAAAAMAEGDEDDGASFGGDGGEAIAEGAEALTSPPGGQRSQTLLHVGDFPAAAVTEVMDRALQDIPAADRAGLEAALEPLLRLARAHAGGRQAYARSEVSELLEAFLAVEEAFSAARRDAGDPGVTTDQEAVDALRKKHAGKLSVVLDLVLSHQGLPLKSKLVLGLMVRLVLPAPAQYRPLLRRLAALTGPSTGELVTRAAQLLEQSLLADLRTVVARSLSGLEMFAGGRSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9K6</accession>
    <name>A0ABQ5S9K6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_009820</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 17">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RPAP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039693">
        <property type="entry name" value="Rtr1/RPAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007308">
        <property type="entry name" value="Rtr1/RPAP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038534">
        <property type="entry name" value="Rtr1/RPAP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14732">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT B1 CTD PHOSPHATASE RPAP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14732:SF0">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT B1 CTD PHOSPHATASE RPAP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51479">
        <property type="entry name" value="ZF_RTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RTR1-type" evidence="14">
        <location>
            <begin position="98"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="242"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="473"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="512"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="533"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="546"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI66109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="557" mass="57631" checksum="2BF069776A37A0AA" modified="2025-10-08" version="1" fragment="single">ITCSSAFPAKASAVGVVASTQSSVSPVTLCASCLGGGRQAVANDAKDEKAKEMEGEKEEEVPPLDSLDDFEARRRRAVFTAMLLLLEHGARDAEHLSHLVAAMSGEELQQVAEERALAGRCGNPLCNALHSVALSPPPRRRWDPSAGFVEPPPPSHFCSASCELLIESYASQLGDSPLERLQPGLREMLAVSHAPRRPGLENVAAQGGGVPTMMAEVRERDLTAAVAEAVTGGRELERLAAAANNDGGGDGGSSRAVEGYIPRAGRSRKPPPPPPGPANAVPANRPQFSPGSTPHNLKPSLRKQSSLKVSGKGADMEGGAATSATSVTSVTVPLERGPKRVTFGPKIEYRMPAQPSRRPASATSQTPLRTTEPSVLIFDVDGANGGGGGGSAKARARGQRVPRQRDAVEAEVGEAAAMGLQGTLRVAEAAEVVQKAAAAEAEAGWPKGLTDEELAATMRERVTPSAVPRAMKLWFPPGAGGRRSGGGAVQRPRQRSKLTVQATGAPLPPCDDPAATAATSTTTTTATTSPSSCDDRTLAATDRESTVSASSFSVLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA19</accession>
    <name>A0ABQ5SA19_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UTP-monosaccharide-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.64</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_010538</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a monosaccharide 1-phosphate + UTP + H(+) = a UDP-monosaccharide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13205"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:140358"/>
            <dbReference type="ChEBI" id="CHEBI:140359"/>
            <dbReference type="EC" id="2.7.7.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the USP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.64" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06424">
        <property type="entry name" value="UGGPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="UDP- GLUCOSE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952:SF9">
        <property type="entry name" value="UDP-SUGAR PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="695"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="805"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="737"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="758"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="805"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="828"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="839" mass="88307" checksum="AA59E023DCD1B2E3" modified="2025-10-08" version="1">MVRKGAILLGVGATVAVGGLGYIAYQFRSNYRSTGISIGEESSYASLKASAPAAAWDDPKVTTDVIAPTDHVQAVLKENAALLDDQDRALVSQLLDLDQAHLFERWPEPGTDDEGKRRMLAQLRMLDASYHGGIAAYVRNARKLLQESRDGINPFEGYVPSVPTGATLDFASPEFMEMEGEGLREAGHAAFVLVAGGLGERLGYSGIKVALPCESATCTPFLGLYIASILALQARAGNGRRLPLAIMTSDDTHGRTLELLRKNNYFGMDEDQITLLKQEKVACLIDNAAHLALEPTDRFSVQTKPHGHGDVHMLLAKSGLAAQWLQSGLKWVCFFQDTNALVFRALLAALGVSARNDFDMNSLAVPRKAREAIGAITRLTRPDGSAPLTINVEYNQLDPLLRSTISKEGDVNDASGYSPFPGNINQLVLKLSSYVPQLAATGGVISEFVNPKYKDSTKTAFKSSTRLECMMQDYPKALPSSAKVGFTTIKQVWASYSPVKNSPADAVAKFKEGNPTHSATTGELDIYKANCLSLAEVAGVALPCPLQTDIFNGISTEVYPRVVLSPAFCPSLSELKAKVAPGSVTLAPDSVLVLDGPDISVRGPLVVRGALVVRAVAGAKVDLGPLTVDNAGWIWEPLEEGEAASEEDRIRGFTVIRKASMELVYDKPGSYIVPAPMQQLSTGETVSVPIAELETGSGRQAGPAPVKEAEVEPTPATQAVERAEEPSAAEVNNAQATEDESPTEDETPADVSVTEGPSAVETAAASRPAAPAVAVASSSCLSAAAKPFVPSSISASLLAAPAATPALPAAPAGSSSKSKSSKRKAKAKAAAAAAGASES</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAB9</accession>
    <name>A0ABQ5SAB9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CNNM transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_010530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="5">
        <location>
            <begin position="25"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="560"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="562"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="631" mass="65685" checksum="FC33CC36B401D2D0" modified="2025-10-08" version="1">MSILGRALLGASDDSDDTSGSAQEDKFLFTLYICISLFLVLMAGLMSGLTLGLMSLDTVELEVLKRSGTPEERACAIKIMPVIKHQHFLLVTLLLCNAAATEALPLFIDRLADPVTAVILSVSVVLIFGEIIPQAVCSRYGLKVGAYSAWFVRILMTICSPIAWPIGKLLDFMLGPDHSALFRRAQLKALVDLHGSGAGFGGTLSEDEVHVIRGALDLTNKVACRSMTPLNKVFMLSTEDRLDERTLQAILMSGHSRIPVHREGNRKAIIGLILVKELVLINPADNTLVSSLRLRELPRLAAETPMYDMLKLFETGKSHMAVLTRAPGAPPLSPGHADGDMASGASVLGGRKPMGASWQTHPRGAPARRLGSGALQNSRDGYVALMDGYGSPGGVGFGEAEPVGIITIEDVIEELLQEEIIDETDLYIDNLQSQRVNAAAVAGSLPPRLRQVLSTGLFTPRVGRLGGGSPKNALPDHMGINSVTSRAGSVTMGAAGGGTPAAAPAILNGDADCVAVASASAASTSEPAEVVGEEPLGLGAAVAAAHTSSLELRQVARDGPNSGAAGFGSANGSRSGASAGLEARSSSGRLSVLRGVGSGALGSGSRAADVDSKVPLLLDANTTRSDAERFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAF9</accession>
    <name>A0ABQ5SAF9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="654"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1370"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1063"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1089"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1114"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1138"/>
            <end position="1178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1504" mass="153720" checksum="8A017B33D2DF7FBD" modified="2025-10-08" version="1">MGGVKAAKVGIKASSKASVTDGNGLPSSSPRVVSEELMALLRRGNASAVKRYLKSGADVNQVLHVPQLDKSWGYAAPAAAASGAAAAAAATAAPAAPAGTASENKRPSSDGRTMATEPAAGAAAAVSVPAPPPSGHWPLILLACAVSSAGVVEILLKGGANPNARNMYGLTALVAAIYNDGLAATEKLALVKLLQRHGFSLFGVSCEALGERATAVTALLHQLAMGEPEAREAAVPLVDWLLQHEPLPHPPDQLLFQHLCFSLALEPAALLTWLPLLQRRTTYVPGSPAYAELVVGVVAAAHQLKDARAAAAAQAMAVPPPSPAAPALPSPSPLASSAGGGWLFGGVADDDLGSSGWAPCTAQQQQEQQRSCGKSGLELSSHGPQLPVKKKYGAVCAGGAGSASGGHGGNEIGTGSATASVVNGGGAKACGGSGGATVRHAVAPTPRTEGSLLAERERSRQGARARVAARAAATASAAPAAAVVGEGEGNPAGLLLRGGGGGSEEALVLRAFLECGLNPNVPMCADGLLPMQRFLEKPELLGVLLEGGMDPRVRAGVSPLDLTFLHSYALIGRFQSGSEVALQACAVLLSRDPGLLWLRCAGLTAFQLALAHANLGMVEYLAPLMGSEQQEAATTLRQRMLGGPIKCCGTSKRKLDNDGDPVSGKRALSIEGDKDDQDDDVAARVWDPASPAAAAAAVAAASAASPVMVTGQDSEVVSSYRLLCLALQRPELWGNDMSGLMRQVPLMDIRQVLTLLDVLNSPNLFNVVAFLEHCRTFAQRVILQVLQLGAEGHELMQAAVPPRAVAVAVAFLRANPDNIWLQSMAKYFPPLVDAMVAGEDTRELMLVAPQMLLARAEEISLLPPDLVQAACESARAAMLLENGFHCPRPQLDDLEGPLRRYMEAGYHRLLLRLLLRLPAASKQVWHDIHVMAYIPEVCAAVALQLCNNPALTAVVAEALTVVQACMAGRPAAHVKRGAAAAAAVAASVPRVGDAVVSAPAEASSYAEAVDAVVAHLAAHLGGMGPANSAAAASAATGTAATVATATAAASTQRLHMPTDDADSAAAAPAESPTISGAPTAAMPSGNTCAPLALPTSPSPATQPAISPCSPSPPCSTRSSSGPTAVPDAPDASIAVTITSSSSSSSSTSGSSSGSSSSDEQGSGGSTTSSGFCSPARAASPPPPLAAAAAAVSADASRADAVSAAEATLSPPSECDCFPPELAYLEDVVVEQTELLQSLYALMAVCSLCSHLLCPDFGLAMCSREFRTRMTGISDAAIDQLAERHGGAASAAKAAGSTAAPAAPASSAAAATAAALLAAYPRSSQLSLDQLYAQLLGSMSPVGADQKELVESRREFLKQVLAMDSMWRAMQQRQQEEARAEEGQQEAEQTIKADGTQDMSAAAETAAAGPQVDAPLPPRRELRGSALVEEARQAVLSHPVIWTVVQQVAALPSAPGPRPGMRVLWDDDNTPIYVLMSLAARRAVHIASGMHPRSLPTEPLQPQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAL2</accession>
    <name>A0ABQ5SAL2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_011062</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000074">
        <property type="protein sequence ID" value="GLI66972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="252" mass="27355" checksum="C40D12BA008D2331" modified="2025-10-08" version="1" fragment="single">LGARLRSNDVQLAEVLNSDLLIKLVTLLGRSADAVLVAVNAMEPCYTEPITPEGLGSGLRSTGLTYLVTRKVVADLRAAVPLLLQLLINLASGPLEGLQVLVRLGAAAAVKQLMRWEDMKAKAAHFLRLLDELDLEYKPFGDWGNGPLTAAALSALQLDPKPFVAQAISSSKLLKLRDHDLRTRFGLDQIGINKVALLQDGHKMFTGIEAMGPKSSRGSISIGNMEQYLVIKHNMREANAKTLSMESFGEMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAZ3</accession>
    <name>A0ABQ5SAZ3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="729" mass="73879" checksum="301CA8F4956A683F" modified="2025-10-08" version="1">MVLQLPSSQLFCYCGMKRRRLGIPCADCGCADSNSPSPAGPLSLNNLPVITASSGRVCTLRRSTTHLAGPAAAGAAVGLMQKVLGSWHYPADCGSCPAAFPHLHGEELSQRARPERYNQQTESSTGKVSEPLTLRPLGLISHDGPMAMPRKRPYSPLDVTDMPPMLSHLAADPQPSGASPQFISLRPIPASEQFARLQGPWSFPADNLETYSCLQTGCLPTATIASTTPPAFASATATTAISTAATITTGHTSIPVSLSASILAKRNSKLAAAKSSLPMSFTTSKAVAVAGGSDTAVAAMTALASVGTGVGAEVIPGWAAATAAPSAQTWFGAKVPLGGGGSSGGGGRGGDELVTCTSNTATSRTTVMPAAAAVPPSPAPPVRATETSLPPALPGQSRPTGRVLPSPGLCDSSSSAGKGSRLLLRLWPLGKQPQAQGGAHIQPPPLQQSQPQPQPQPQPQPHFIMETGAVSNPQYGAGVIIESPLGPALPLARLEWLLQAAEAAAAAATACTWSLTHGTIAGATAEAATVIGMALEASACSGCLADVGGGGGSGSSRMAAAALPALRVDPLEYFEVDGGLLKGAVLQTACNIIRLVQLSNSLAIVPPWAPAAVQDLRATAAAASTFGDLYGKLSGSGTGGGIGHAQFAVQPGQRRGILPLVAALKVLVAFAGASRALQKAQAECTETTAAVAEGLTALLPLMRRGCACADVLMEKVERVLTEGPRLLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB48</accession>
    <name>A0ABQ5SB48_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alcohol dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_010877</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the N-terminal section; belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000045">
        <property type="protein sequence ID" value="GLI66879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08178">
        <property type="entry name" value="AAD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07122">
        <property type="entry name" value="ALDH_F20_ACDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034789">
        <property type="entry name" value="AAD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012079">
        <property type="entry name" value="Bifunc_Ald-ADH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010378">
        <property type="entry name" value="PRK13805.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF83">
        <property type="entry name" value="HYDROXYACID-OXOACID TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000111">
        <property type="entry name" value="ALDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00060">
        <property type="entry name" value="ADH_IRON_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="87"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="10">
        <location>
            <begin position="532"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="11">
        <location>
            <begin position="720"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI66879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="856" mass="92415" checksum="48F08D6E4032E52A" modified="2025-10-08" version="1" fragment="single">MMSTRFNPCLKGLGTSSGLLLMPARSSRRPAARVMCEAAASPAPVTPKPGSTAVQAQAPVTTPIAHDGAAKKERPPVADDQLAGLKSLLKLSLEAQRKFSIYSQEQVDEIFRSAAEAANAARIPLAKMAVEETRMGVIEDKVVKNHFASEFIYNKYKHTKTCGIIEEDQAGGIQKVAEPVGVIAGIVPTTNPTSTAIFKALLALKTRNALVLCPHPRAAKSTIAAAKIVRDAAVAAGAPHDIISWVETPSLPVSQALMQAPEVSLILATGGPAMVRAAYSSGHPSLGVGAGNTPALIDETADIKMAVSSIMLSKTFDNGVICASEQSVVVVGKVYDEVRAEFIRRGAYFLNEEEKAKVRAGVLIDGRLNPNIVGQSIPKLAELFGIKVPQGTRVLIGEVEALGDSEPLSQEKLCPILAMYKAPTYEHAVHIAHSLIMYGGAGHTSVLYTNPLNKAHIELFESTVKTVRILINTPASQGAIGDLYNFHLDPSLTLGCGTWGSTSVSNNVGPQHLINIKTVTSRRENMLWFRVPPKIYFKGGCLELALRDLIGKKRAFIVTDKPLFDLGYADKVTRILDDINIHHQVFYHVTPDPTLACINAGLSEILEFKPDVIIAMGGGSPMDAAKIMWLMYECPETRFDGLAMRFMDIRKRVYEVPELGKKATMVCIPTTSGTGSEVTPFSVVTDERLGAKYPLADYALTPNMAIVDPQLVLNIPKKLTAWGGVDALTHALESYVSICATEYTRGLSREAIQLLFKYLPRAYSNGSNDYEAREKVHYAATIAGMAFANAFLGICHSMAHKLGAAFHVPHGLANAALISHVIRYNATDMPAKQAAFPQYQYPNAKHHYAELATMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBB4</accession>
    <name>A0ABQ5SBB4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011170</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF25">
        <property type="entry name" value="MYB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="10"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="540" mass="54238" checksum="8A32B9291D96A0E8" modified="2025-10-08" version="1" fragment="single">HIATLLGDAQDAVILWAHMQYGNKWASIAKHLPGRTDNHIKNRWNCTLKKKHADIMAQLRAETGPDGVVSIAAIHNVLISQQQGGGRGPIIRGNGPNSLNKGAAAFMSPMAGLGALGRDTGGPQALLPPGLPSALTGALGGALPGGLPALPPALGAGLPGGLSPMMLGPMATAVAADQERRLRLAGGGGSAFSAYGNGGQKPSDEQRGVKRRRDGEQGTSDAESDEDTAAVALKQLAHQDPREGNVEEDGAAGNNPNANNQNNSNNNNNNANNGKAAAGGTSAGSTEDEDEERQEEDGAAAAATAGRDAAAVAGREGEEGGVEDRQDNETTRGKGGDGSSGRQGAEAGPSSGSKAQRLAGSLSAQIKSEASPAGANAGNNIAAGAKGAKPQPGPSLPTGFAGLASAAGTGPASDQMMQNAASANVPLPNSAAANYGQLLNQLPETLMQLSSTLRVVLTAGNIPAAQALVSKLEELAYSVLMQRAAFNLATAETNTAQQHPLQQHTTHTAPVQLPGQGVNGGGVDPQILAQLLLLSAQGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBF1</accession>
    <name>A0ABQ5SBF1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ubiquinol oxidase</fullName>
            <ecNumber evidence="13">1.10.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_011455</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>2 a ubiquinol + O2 = 2 a ubiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30255"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="EC" id="1.10.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="13">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the alternative oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.11" evidence="13"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01053">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.140">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002680">
        <property type="entry name" value="AOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038659">
        <property type="entry name" value="AOX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803:SF19">
        <property type="entry name" value="UBIQUINOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01786">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 13" id="KW-0408">Iron</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI67272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="471" mass="53968" checksum="131EF4D269B024D6" modified="2025-10-08" version="1">MLPVTLSQKQVRFGNWSASSRATAAARRSLRVRAVAQVPKGQNEPRTEVAVPGAKPPVVRGNVFFTTSLDSVSTDEECSAFIDRDGEFVEVLCCDYGFRTGASRMYSKDADGRIPRNAFQMAWSNFQQELLALRQSFRFDDFALISEANPPRSALRRAAYGVGQAVVDGLGALDRALEKSQVLDKLRPRPITIDVIGDRSKLLNECTETARTFTHQVRAKLKQLQLSNQAVWDREHARERAGQAVETPWFIKGVYLSLCLLLDVLFENRPIQRFWFLETVARMPYFSYISMLHLYESFGWWRAGAELRKIHFAEEWNELHHLQIMESLGGDQLWLDRFCALHAAVVYYWILLGLYFFSPELAYNFSELIEAHAVDTYGEFVDANEELLKSLPPPLVAAMYYRSQDLYMFDSFQTSQPLLNPRRPACNNLYDVFCNIRDDEMEHVKTMRACQDATVQQDIVSRREEGPRIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCT6</accession>
    <name>A0ABQ5SCT6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA-processing protein EBP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_011431</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008610">
        <property type="entry name" value="Ebp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028">
        <property type="entry name" value="RRNA PROCESSING PROTEIN EBNA1-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028:SF0">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EBP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05890">
        <property type="entry name" value="Ebp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="210"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI67249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="37276" checksum="D1D81E3D2CC55851" modified="2025-10-08" version="1">MGNKRRKVAEPESESEDEEFDEDIQDADDDGDDDGEDTGQKGRKRQVIYDVDALHERLEDIGWTTEADWEESLAVTSEKPTVVGNVDDDLARELAFYNQALASAKHAIHRFEEAGIPWLRPLDYYAEMVKSDEHMAKVKEQLMFEQRQIEQAEERRKQREAKMYGKQVQLAKQKDRNAEKKRQITEISKLRKQREKSGFDGELDMDAQLEQLEQRRPLNLKQLRQRGRKDDKTPNKKRQMKDAKFGFGGRKSLKKQNDAYSAANMDDYKPGRFKGIFGPRKGGVQKKFGAGARAGAGGKNAQKGGKLQRPGKQRRQAMKGGKGGMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD31</accession>
    <name>A0ABQ5SD31_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011783</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.120">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050641">
        <property type="entry name" value="RIFMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004:SF6">
        <property type="entry name" value="FAD_NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="240"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="492"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="677"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="185"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="651"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="897"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="989"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1076"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1184"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="652"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="905"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="990"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1076"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1107"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1127"/>
            <end position="1136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1257" mass="130288" checksum="ECFBA86D63EE3429" modified="2025-10-08" version="1">MGIIEGVRRNCVCFRVATAATATATATATAAPAGVDAWRLASVFGTAKADMDLQLRQVRGHDCCSRSSSSRSSSHNSHSQCRIDGDGSYGEVLHRQEGWTLPGSWTNAAASATPAVLNRMAFTVTTVPTVSATSCKPWRSGPTARDASTRSPPQEALLLRADRNTDGLRRRSLTANVLSGCSSCRGYNSSSSNSSSSSSSSRGLVVGSSCGSGGGRAAEGGGPSLEVCDEVAVEEVPVPVPVVICGAGPTGLTLSLLLAKYGVRHLVLERAMGPTQHPQAHFINNRTMEIFRSLGLATPVRQQMPPLEQWRRFVYCETVSGRLYGEVDHFQGQSSPLTPSLSPEPVAHLPQHRLLKIMLQRQGEVAAAAAAVSSVGGGAVASGNDAVQKAVTDTTKASQLPLISVLGGEDGRPRVTKGGPAAAAAAAATAAVSWGSVLHGVSFRRRQVAADVGAASASTGANDLHDSTDGEMDVEVEVEVGLAAAAAAAAAAGEMAPVRSAQRRRVLLRCQYLVAADGAHSTVRGLMGGNMEGEGPMQHLLNAHFTSSVLARRASRRPAMLYFVFNPEVVAVLVAHDIEAGEFVAQIPYFPPLQSPADFPPCRIVELIRSAVGQTATDSEEVVDVQLLQLRPWVMTAQVADRFAFGSCCGSSSGGGRGSGGGRGRGRGTSRGSPPVVFLVGDSAHRFPPAGGFGMNTGVQDAANLAWKLAAVLKGLAGRELLESYQVERKPIAQANTALSVRNWNEATRVPAALGLDPRLANAVSSLVANAAPLPQWARKGLLEAALDLGRRMAGPHGLPLPLLGTWRQQQVQRILSSGASLRLQFPAEDLGFVYGKGAVVMEQSYMSRTPAGSDGRLSHTEMAAAAQLPPRGAPYIPSSAPGCRLPHCWLHVPEPIGGTGDGDSSSSSSSSSSSNYSSSSGRSSKLTGRVPPERWRGPVTLSTLDVVADPWVGPRLVLFTDGQPHVGNTWLTAAQLLYGGHTLMPPSTPTVNTPSPAPSSRVFAPGPSGASGKTPASLVVLQVLTATSAAYNTSGGERQQWPKMTANLSCPMAVVIHEPPKCSMLGPAWSYGTSPSVANSSSITRNNDVGLCRPNSDDEGYTSSDRTTRGDDHVGKGAGKDGPGQQGPSPIPGVVGAPLLELVELQEAEPGSWLRTMGLPPGSCLLVRPDSHVAWRHLGPPGGKGALLSPSEPSTEADASHPAPSPAPAAAPVAAPVPAATGAGNVVDTAVALAVEKLRSVLVALHWKAASAAIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDR3</accession>
    <name>A0ABQ5SDR3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="847"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="311"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="853"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="926" mass="101739" checksum="A4ADE8EB229AD0F5" modified="2025-10-08" version="1" fragment="single">LQPQSQSSAAVNAAVAEAYGRRGRWPVEVVPLLREATGLLAMPLLGTASGAGTTSNVSRLFSRDLTLAALADQLQYLSAVWCSPGGSLRLVDSRADREFEGFSTHPQAKQFKVQQNVLPALYNAAQAKVQPPSAAQRGIMGQQAAGQPQAADAAGLLHAAAVEAVWVVASSDYRPTQDEQTRQSDAAALIQRSWRGWRRRQEEVAAVERQRNREEDAIRLLREGMSLQLRISLKVYFTRLRRSLDARKQQEAQAAAAAAGAAGGVGEDGQGDEFGFAATERYLQQLETHPYIDEASCPVCQPKHLAQQQQQLALQQQQQRAQEEKGQNHEGGLTNSASAAPNVGPPAAVRQLPSLNVAVADFAPHKSRIKHIEAVELFRKCCELYRQHVVPLMRWARELMAALENVRAEASRDEPDKFMVFTMESSMALEGALEQLCQQVRQVLADRLWKASEELLAPKWYECKNACLASYELLMNWKSAYDHAMLTHPTAFGRQENWVPRDQQLACMSTAGADEIEAQSALQQQKPQPNEVPIPRLLLQQLHGQHHPDNGNSIHSEQHRQQQRHETTTQQQEMTYQPLLQAQHGNAQQQVQQQVQQQFFYAQEHQRQQQNEAWYPMSQSQFNEPQRQWHPPQHQHLQRQGGWGNAWSMQPGFLHHNTHLQEHHHQLLPVARTGSLQVLQVPGAVPGATPGRALTRPVMPSRASYGGARMQGHQAFASGGPPTTAVPWVVNLSNPQYPLLRWEHHQYHQHRALCDELPPGLNLGGSAASAVPAAGAAMAYTNVVSDGGTAIAGGGSGLRIEEELMFPEGLGGDDGGRDLDIGVDIDIADDLGDEEYAEDRRLARRRRNRYKNGNGGGGRKGGGGAGAANATGIPSSPGPAAGLSTRRQQQQQQQQVPAVLRGNAFAPLIHGNATLPRQNQGKKGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDX8</accession>
    <name>A0ABQ5SDX8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="164" mass="17184" checksum="589EB5610DC05C28" modified="2025-10-08" version="1">MDPAEKAWLTAPLPNPTLRAGKPKQRRQRDDDNDDMGAGGGRKKQRRESGGRARGRGASTGRRGMHQAASNRRRTGGDAEEEDDFTEGDAASGDTSASSGDMAPSSGVSSGTGSSGGNAHQPVLRSSRRGTRGALLPVTARTKQAVCDKQGRSSRRPRGSRGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEE3</accession>
    <name>A0ABQ5SEE3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012668</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="518"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="643"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="918"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="986"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="475"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="832"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="548"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="574"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="651"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1043" mass="106792" checksum="DDCF40900E7A28D7" modified="2025-10-08" version="1">MGRLSFKRKSKTSSPTAPSPGELSGSPSSVPEQGNGDAHLVASSNSSPTSGVLKDATVYKSYSTPAKANRSSVGHGSGNDNADSRMFAEQLHRLRTRLQSVVQGVRDVAQSLDEPHSLISNVRDQLQTRQEAAVARRNAASTLATGVPERENAAIATTGPVTDGVSGGATGAIHTGVSAATQTAAPLSRTSSHGSRNGSSLHHSKNEAVGAMEETSFAPAASVSSGPHGVAALMALEGTSMLADDGVSCSANLSVKARVAEVERRSRSSGGMSEPLPGLAGPMPPHGVSAEGSLHRSSTAQASLLVARSFGNGRRGSGAAGAEEAIYADSPIRGFSPGLVPGSPKKATSLGSGPNSGTWRPVIVPPVAQTPSMAAIVAPSESKSHSLMGTDPVSRNASGDGALDATAALAAADIIAADAAELLGSGDDSLLAATANPAAAAAYLREHPETLRELWTLRARVRAQESEMTSVVIQLSNKNAQLDQAQMSLQAKERELAAITQRLTAVLAKLQSFQAAHGQEAQQPDQVRRASAGGVSASTGGVVGGVHGSSHAPAAPAAMTPPVAASLPDRRDNAVSAPDAAEAGAAKAGSSASLPASSTPQTPLRNAPLASSSASVMINNSHFAAAKAALQAEAEAAVVAAAGPSRAPRNTPSADASSAASTTASAAASTTPSAVASATSSPEHQQGGPRGTLEVSLPPPPSVRAPLDSNSQILAEAAVAEEADARALEGGGSGHVVDAATATASCARLPLSVRSPPSPSVAVSGAGSCVRAVTLPSGSNVLPSATSEPATVPPGAETASDVHIAEVTTVLRELSDVRGRVQAQEIDLALMMEELSTRTAQLDQAHNTLQDKERQLQEAAQRLESVIAQSRPQTQRRRNGGLLRFLLLQLSPSLACIGLLLRDKAVLQRLQQLGRLRVGPPRPARESLFPSPPPSKMSQPELGHRLLLPSPQRHVPPPQAPQLKSEQRPASRVMRVVAAVAAAARVRPAGAHRRRDTAPPLTSSATPEPLMLAGVDDRTRWSTECKSQWCKIIRPPRQEAVYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF37</accession>
    <name>A0ABQ5SF37_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="4">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_012094</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000741">
        <property type="entry name" value="FBA_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033379">
        <property type="entry name" value="FrucBisAld_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00274">
        <property type="entry name" value="Glycolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="360"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="383" mass="40035" checksum="653B89FBF1C2CED9" modified="2025-10-08" version="1">MAADLASVAAKLAAPGKGLLASDESTGTIGKRLEKAGLPNTKDIRRAYRELYYTTPGIGQYISGVIMFKETLYQSTAAGRRFVDVLADEGVMAGIKVDEGLAPLPGGGEGETYTKGLEGLEAACREYVMAGAKFAKWRATLKVTDSLPSDLAVERNAEDLARYAKVCQECGLVPVVEPEILIDGAHSQTRFGVVTERVLGATVAALWRQGVALEGCLLKPQMVIPGAEAEGGKVAAEEVASHTVAALRRVVPPAVPGIMFLSGGQTEEEATINLDAINKEARVVGRCPWALSFSFGRALQASVLKLWSSDQTRVAEAQQLALALAQVNSEAALGQYTAAAGGHPSTLSGDSLRENFRGWSGQAPAGGAVPSPGGSGEEVLMAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF83</accession>
    <name>A0ABQ5SF83_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosomal protein/NADH dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_012324</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016464">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B8 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005822">
        <property type="entry name" value="NDUA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI68002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="101" mass="11111" checksum="2FF8B891D3674F90" modified="2025-10-08" version="1">MAWRNALSKSMQELRIHLCQASEASKGVREFVVSNYAEMKKVNPHFPILVRECSGAEAKLTARYDFGAEKTVSVQGADSAAVLLKLQELIKAGEKMPKSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF93</accession>
    <name>A0ABQ5SF93_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012463</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044159">
        <property type="entry name" value="IQM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31250">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN IQM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31250:SF27">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN IQM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="304"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="431"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="494"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="621"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="705"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="890"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1060"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="304"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="495"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="511"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="718"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="736"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1081"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1415" mass="148196" checksum="4287ADE3289C42F9" modified="2025-10-08" version="1">MPQILSLVGKALGNILRSSGSNDSAGRYRNSVDSDGGFDGPKRCRGSAAGASPASEPCSRQRISYQNECLENLGASASQSADLFNAPEKDVGPGLATVGSSGCGLISAGRSSSTGDAGNAAVASATGTGCVCSDAGFPGSNSPPAATHFFRASRASLSDTTSSAAGEALRQASVTTCKHFSRENYNIYVRWVRASRGAMKIAGKLRSREAVCRNPSPTLLLRCEHWLEVTDEQHRYGSNLRVYFDFWVAEMEAAELTPPQSPALISPSPSYSQLVALAQQQQQLQQQQIANAITKAAAAAERISPSQSAHQYNNPRGQRAASAAQVEFHATTPHQSPVGPTLHQQQPVGQPEPQQHAAVASTGQCIGANQGMVPGPGPSRFSSDANAVPRGNSASERRSCDQLPRRVEEQALMPLQSFGSEVRVQHLSHRYPHHQHHYHQDHQQQQNTSALARDRCGTGADGAEGPGDGDRVLTGCSVLSCKSSHLWDEAERQQLKQQVGEGQQLQEGPRAHPHYHPHHHQHHYRRSHHHDLELYEREGSVHHGTDDGGADSLPVSLTVTRSHDGDDDARGCIEPLSPTDAADVTSTGGDDGGELVCCMASSCDPTPTLLQRRQQLQQQLRLQQQRRHSCGESDAQAARHSTAGQPRPSSDAIAIIPTQSVASTVAGPPTSPYLATGSPRRNVLCSPSRRFVLAAMIDMSLAEGQAPETPAPESGRHGLQQAQQAQGAGEPVTEGQIRQQQQQPQQEEDLGTATAAETRVSCGTEGGSGTPATPQQQQQPEGASLLPVTPALTSCGGSASVGGAAMGASGGCTAQRAAKLMSNRASVGTGTSFFRWLDYGPGSDVDLAHLGVSRAKLDTERVKYLTPNELLEYELDVDMETGLLRYKRSGKLLHTGPDGRGSLDEHRRPPTPPPPAVPLALLGEGAAAWWRSSSTSAIVEMPPMPQVAGIATIPPLPRKPAAVARQAFRRMIAMGTVLEGGSGDGCSGSSSYASGFEASRQPLLSGDDAIEAAAAAAVLAEADAHDKAQGFSISTPVAKAGFLPDLELRGHSASSLARLEPSGTECYSEKGNFEEAGAGGSSSSTGDSCGNSRPCSSAGVGRAAANAAAAAVMALSISAGGTGDGAGPGCRASLAAEAQKDDVETTNGITFAKSGDVSSAMVLGPLNGRMEQQPQVLHLPGVMTPPRPAMLARGDVVSTSAVAAVAGAFGSPNAVDCAAAGSMPKTPVATQAPGCGVMVGGGYHHDEKVAKWIYVVDPALRLFVHPKVRGRFHHSSFLRGGAVVAAGGLAARHGRLRLLTADSGHYWPREENFRWLCEHLVYVGADLSVCELRSKHMPVPAATGKELMQKMGVPPPWRLPFQTALSPSGHLSLEPASEAEAPPSPLTEHVRLQQLHATVADGETELGGAPLLSGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFG1</accession>
    <name>A0ABQ5SFG1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="218" mass="23953" checksum="300DC46D73F20488" modified="2025-10-08" version="1" fragment="single">HVLLPKSQSNGPLCPAPPSFCQPPPPPPMLQPRPSYGTAAMCPVGTHVQEDYWHEVWALRTVDPLTGEDVIVMSQHDVSAKVIAERHLALVMEAEHRLLEQLFPKHILTHVTEEWIAEAAKADEKAAGNKTLECGTSAVKDTTGAEAISAAARVNNPRATSNAEWRPVVRDCNALATWHPQVTLLFADIKGAKIRSLLERTRDCIIHCMYVLAAHTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG61</accession>
    <name>A0ABQ5SG61_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pirin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012699</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="3">
        <location>
            <begin position="31"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="4">
        <location>
            <begin position="181"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI68374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="32013" checksum="84CF2AF7595DCEF9" modified="2025-10-08" version="1">MAASTLANLRPVTKVVQGQKMKEGAGVTICRTVGTSTLRNLDPYLMLDELKLPAKAAFAGFPDHPHRGFETCSIMLEGQMEHRDSQGNQGVIGPGGVQWMTAGRGIIHSEMPKSTDGMLWGFQLWINLPKKDKMCKPRYQDYQQRDIPVVESSPGASVRVMAGSHGGVIGPIKMRNPGLLMDVRLGPGANVAQEVPLGWNGFAYVYDGEGKICGSAAQREQALVLGPGDHVTATGGSGTNGLKFLLIAGKPIGEPIVQHGPFVMTSQEEIYQAFRDYQSGRLQDPADNPWKGDDGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGC0</accession>
    <name>A0ABQ5SGC0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF77">
        <property type="entry name" value="FI19380P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAS1" evidence="1">
        <location>
            <begin position="20"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="1">
        <location>
            <begin position="199"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="362" mass="38476" checksum="C11266EAD9882B1D" modified="2025-10-08" version="1" fragment="single">IGTAGASPLLPLAEMIGCEGNSLMVAVMKAPELVTLRSALNVAGLHPRLNNDRVNMTFFAPADGAFTQLAADSGVTLEALLAEPLTLRHLLLYHLLPGPVPPDLLVVTPAFSTYWAGENVYVTQPFQGVVVGESGTATRISKAVRVCGSYLYIVSHVLLPGPSLEALPPFIEYTKPTLNLAPVTTTTTATSSPRSCNPDGTILDVLRSNPDLSYTLGAIDTSRLTSLFNNASVEMTLLAPNDDAWWEAALRMNLGTPEEILGQTMANLRALVWAHVIPANLPPTKLRPQLYESSGGPATGPISVNISPGNLTMQTPTTDAHVVAMGLGDACNAAVYVVSRMLVPQQLPDVLKVQPMPTEKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGS9</accession>
    <name>A0ABQ5SGS9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="3">2.5.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_013614</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090">
        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090:SF5">
        <property type="entry name" value="PENTAFUNCTIONAL AROM POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Enolpyruvate transferase" evidence="8">
        <location>
            <begin position="72"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI69070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="428" mass="45525" checksum="5467149CBEA7506E" modified="2025-10-08" version="1" fragment="single">MLLGQQCSLRLRRVVNTQEFVMLPVRPIVVAIPSQRVAPASAWAPAPKHSSRRSAFACAAATKEKVEELTLQPVKKIEGRVKLPGSKSLSNRILLLAALAEGTTLVKNLLDSDDIRYMVGALKALNVQLEERWEIGEMVVHGCGGRFNSSGAELFLGNAGTAMRPLTAAVVAAGRGKFVLDGVARMRERPIQDLVDGLVQLGVDARCTLGTGCPPVEVNSNGLPTGKVYLSGKVSSQYLTALLMAAPLAVLGGPGGDAIEIIIKDELVSQPYVDMTVKLMERFGVVVERLDGLQHLRIPAGQRYVSPGEAYVEGDASSASYFLAGATITGGTITVEGCGSDSLQGDVRFAEVMGLLGAKVEWSPYAITITGPSAFGQPITGIDHDCNDIPDAAMTLAVAALFADRPTAIRNVYNWRVKETERMVAIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGX2</accession>
    <name>A0ABQ5SGX2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">lysine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Lysyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_013198</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034762">
        <property type="entry name" value="Lys-tRNA-ligase_II_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001756">
        <property type="entry name" value="PRK00484.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF9">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039101">
        <property type="entry name" value="LysRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="288"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="22"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI68713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="623" mass="70839" checksum="B7BE2C7C09D771EE" modified="2025-10-08" version="1">MVTTEYVLPEQWSEDLKDEKGELMSKSEFKRRQKAQRVAAEKAEKEAAKAAAAAAAPPKPAKEDGPALEDDSNEITDPTAYFENRVRYINAKKAKGVNPYPHKFHVSMPLPEYIAKYCSLEPGQQETETTVSVAGRIFSKRASGSKLLFYDLKADGVKIQIMADARCGGKARASPCEMSRYLYYRCLCPLVDLNHLGFRNSELSLDEFTAVHNDCKRGDIVGVEGFPGKSKKGELSIFPRKFVLLSPCLHMPPSTHFGLKDQETRYRQRYLDLIVNSDVRNIFQTRCRIIQFVRRFLDTRGFLEVETPMMNMIPGGAAARPFITHHNDLDMKLYMRIAPELFLKMLVIGGLERVYEIGRQFRNEGIDLTHNPEFTTCEFYQAYADYKDLLDMTETMISQMVLEIKGSYKIQYHPEGPEKPTIDIDFTPPWRRISMVSGLEECLGVKLPTDLESEEARQVLVELCIKHNVNCPPPQTTARLLDKLVGEFLEEQCVNPTFICDHPQLMSPLAKWHRELPGMTERFELFINKREVCNAYTELNDPIKQRELFQDQAKAKSAGDDEAMFIDDNFCTALEYGLPPTGGWGLGVDRMTMLLTDTCNIKEVLLFPAMKPEETGPKKEGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGZ6</accession>
    <name>A0ABQ5SGZ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Survival protein SurE-like phosphatase/nucleotidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013341</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="5'-NUCLEOTIDASE SURE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457:SF0">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G01070)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="5">
        <location>
            <begin position="45"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="447" mass="47541" checksum="431C41BF1E4DA1D9" modified="2025-10-08" version="1">MRRIFMSKSSASILLRQHRDRPCSHFTLTRSRSSIVAVRAAEPCVVLSNDDGVESPALQSLAAKLRQDTGQRVLVIAPARNKSAASMGLTLRNDMELRVRPDLGPDTYALAGTPTDCMMVALDATKGLLRALDLHPIIALSGPNYGPNMGTDVLLSGTVGAARTAGLYGVPSLASSSTSTDKCSSLDNAVGATLALTKVAMAALGDRPARNWPRDHVVVGGIGRRHVAATCEYESLPHNWVMDVEQVLVDAFQDGDLFLNLNVPADWQPARGRVQTTGLGLMFYRDSYVVSVPERSSPAAETMAGQLQPALVAAIEMPNGNGNGNGDSNGGALSSRGVELANGSGNGKINGNGNGTRTDVIQGIAEALAAGYPVVYNNAYDTRRIMQIDRSDVMALQAGHVSISTLQTWPEGHTLCLSDRVMSRALLSGHDGMPAWLQARREQFQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHC1</accession>
    <name>A0ABQ5SHC1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013928</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21608">
        <property type="entry name" value="RRM2_NsCP33_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048289">
        <property type="entry name" value="RRM2_NsCP33-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01228">
        <property type="entry name" value="EGGSHELL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="55"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="201" mass="19998" checksum="FCD9BA59A4017636" modified="2025-10-08" version="1" fragment="single">VAIPFMLSSRSLVFVSQSARRVAPFSASLVQRRLHTTQASASSTGKMTATGNSTTKLYVGNLSWETRADDLNSLFSKFGAVEDAFVATDRETGRSRGFGFVTLESTAAKAAAVGIDGSEFMGRTIKVNEATPQADRTGGRGGGGGYGGRGRGRGGYGDGGGYGGGGRGYGGGRGGGGYGGGGYGSGGYGGGGYGGGGYGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHG7</accession>
    <name>A0ABQ5SHG7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_013615</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="entry name" value="Ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_bL17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047859">
        <property type="entry name" value="Ribosomal_bL17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_bL17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="Prokaryotic ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01167">
        <property type="entry name" value="RIBOSOMAL_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21705" checksum="3D0DCD6E044FAD59" modified="2025-10-08" version="1">MARVRVRLGNWNRLNRKWEHRVSMMKTMVTQLLEKERIRTTLAKAKTLQRYVDKVVTLGKKGTRDAWIQARGIVRTDRELHKLFTQIALRYRERSGGFTRLVRAGWRERDAAPVAFIELVDRPGELRPAGPALQRVDPLSVRAWMQMQLGQTPREASPLLPTVVRQYLRDENRIRDRTNVPELGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI11</accession>
    <name>A0ABQ5SI11_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4042 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013384</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025283">
        <property type="entry name" value="DUF4042"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052107">
        <property type="entry name" value="HEAT6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13366:SF0">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13366">
        <property type="entry name" value="MALARIA ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13251">
        <property type="entry name" value="DUF4042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4042" evidence="2">
        <location>
            <begin position="432"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="724"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="780"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1015"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="724"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="785"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1035"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1118" mass="115870" checksum="B2BBE66B5F945051" modified="2025-10-08" version="1" fragment="single">MSWRSRGSGLSAWRQCLAPLRQEAAVKGYVDRQNLQAALESLRTAATEFHENEFAEDLPFLVQQLLGWQPSTGTSISAGFPRDNAPQVALCRLLNSLTASRTISLHRPTANLVLSALRQWSDGALYAPKQGTSESQGSNGVANSASNPVRTAATSSSVDNSRGISGCGNAAMTADSPESQAQLSQPPSSKASLPLEAMTALAALLPDHLGILPEHERAAQLAALLSLLVPQPRGVEGSRGRGAAEHLEMQILALTALGACCSRTAGSGGSSGSGLTTDEIAALLSAAVSLLQSCASRSRPIEDAAHSRMYAALVRVLQLAIGEARQTWVARAALLVEGLQRFLTYGVQASIAAAAPLVSSPSPASGLPQPTETNGGRYRPPHLRRRESQADGGLAWSDSDMSDTEHGGGASSTAMGSGAGSMAVVDKFRSARVRQAALTCIQAMAKGDPRAMHTHWSALLPLQNPLQPRPLTPHLITVLLYDPLTKVRALAAATISALLDGPAQRGILSVAEAKPVSARAPVRGFLTLSLTLGQLLLAAHTGLLHAVATESSMLVLPGVLRTLVVLIQVSPYERLPQELLPETFKVLQTRWAELTAAAPATASVRGGDLGSSQTAYLACLAECFSTKQPVAGLAAFLSQQQQHTFQDLKVLESQHGGAGCDSTQRLVRDLLAVGQDASAEAVQRIEALAALKGLAVNYTTALPPDVWDDLRATAAMGLSCIPAVRSPPVSRSGPPSRSSSWSSLGAVATHGASGKPGDRAGLRVPASQAFGPGAFPGAAAGGAVSQATSSPITPSANSSPEDKAAQLSVRLLSDYLSAIARQCDIILTDSAGAGSGDGGLPGLPPSPGFVPLAAAVARQHQQSLSPVCVQSEEQRKAGLKRLTSMWRDAVAVLVPTATSHQSYMVRSAGLSCLSEVPEPVFSSLTVALQQHLLTLFSDAAASDAVAAVRAAACKALGAAAVFPAVLETPQLSDHITRALLPSLHDAVLSVRIAGGWAIANLCDAYRRRLEALEAANGEGSDTEQQLRSRVDSHAHASTSSYDGGSGAVSSHKQQTLSLATLPLEPHHYRQLAALCGAAIISTQDTDKVRANGVRAVGNLLAFLTPDMAPGLMAAGAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI72</accession>
    <name>A0ABQ5SI72_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_013962</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12232">
        <property type="entry name" value="RRM3_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="236"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="377"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="429"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="103"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="377"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="437"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="451"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="584" mass="64089" checksum="EF84F16458841D55" modified="2025-10-08" version="1">MGDHDDYPYEHDRLYQAERGHEGGRMKSRDDGVSRDFRERDRYDREAPRDYDRRREERDYLDRRDDDRRRPRGDDRYERGPGAYRDYGDRRDVRSDLRDYGRERRRSRSRDRRRSRSRDRYDGSDRREGGDDRDAGRRGRGGRGRRDEYSDEDDAYGGYKPRRRQAPPDPSKYDKPLPPGAGPGYISDPFYYLRQNVPADPAEAQRLWLEQQLRSRQMVLQQQAASAAAASAKTQRELYIGNLVPGAVTDVALRQLFNTTLAAAFPVTGGAEPVVNVNLHSDGRYAFVEFRTPEMATAALALNAQVQLLGQTISVGRPSGYVDPHKAAAAAQAAAQALAAFQAGDMAALAANATAAGMNLAQLGLANLVLPKAAGGATEGTDAGAAAGEAGAGPSREQTPTAGIAGASGTSNDVEKEQQQLMMMKLLQSHEKEQDAQEQLGSQEQQEQLEDTSRDGPEARKANGGGGEPGNAPVATQFFCVLGMLSADMLVDDEEYDAVIDDLTDECDRHAPGGVVAVKVPRPPEDMRARSNEIFGTGNFGKAFVSFKDAAAAQRAHAAIHGRLFAGSTVLVQYITEEEFDAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIE9</accession>
    <name>A0ABQ5SIE9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hexosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047125601" description="Hexosyltransferase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="42681" checksum="7419375C04A1720F" modified="2025-10-08" version="1">MYRFIIFIIINILFESLNVCNAGQQPQTLDLSEGPRQGLMDAATIAAQTQLGVSSNNVNKLAMIYLFSNPHSGRMNFLRASIGLCSENLFPTTPTIVYVFTQPHQVETMKLELGKWLLPNVLVVPITNDSWQMHPSSREERSWHGKRWYSSDYRIMGNWRLSYMPAFARDMGHRYMLQIDDDSFILSPVGFNLVEAFDRHGYLMAARNIMTDHPYVTWGLPELARYYLVTHKLVPATLFEHCNPPNLTGLYSQFSDKQMLDELSPEEVHALEELKLPSEGGWDMTILYGNCVMWSLNWWFQPEVNHFVQLAIASGSTVRERWIDQAVIAMIWQMFVRKDQFLQFNFTYEHTKDPRIPNEFLSKHNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJL8</accession>
    <name>A0ABQ5SJL8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000091">
        <property type="protein sequence ID" value="GLI70188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="123" mass="13132" checksum="09B2BACF859CE10E" modified="2025-10-08" version="1" fragment="single">MDNTAGPRVLPLPQARGHDDIPEDPLGEAVALDQELRATRPTPTAATRRSNRQRQARGGASSGVRGSGGAGPSSDPAKSRKRGGAPSSAAAQRSDPNAKRTRTDDSFMPKWNCNRSKFERRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJX7</accession>
    <name>A0ABQ5SJX7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="41391" checksum="5A3F1EFF99A91697" modified="2025-10-08" version="1">MGQAWRIVMENVRQKVGTDRTFKATSNARNQAMFKEATEFCKHLRVLERDLRSIHKAIDAKFANLRNILCSPLPRAFEEGRDGAVPCTEEPKLIGQGVGLEVLQQAANDLKIRLDEDVIKPLRSWLIAYRSVRDRMEKLEALRLELDSRRRTVDSLNEKVDRLDRMQPAPQQKDKHEQDVEKTTHLLHHKGEKLTRTMHAYRELELAVFNSLNTLIKDTGVLRDYTGLSLQIVQECYQKGYAAFSTATPLLDYNSTSDNPFAHMQMIDNITARVGAPSERSRQPSDRLGARKDGLGMYAPPSDDVGDAGQYGNDQQIQPQMSGYGDMQQPVNPYHGAYQPSPSAKRFMTSPSRSPAWQDQVMQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK64</accession>
    <name>A0ABQ5SK64_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nicastrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_014585</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nicastrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041084">
        <property type="entry name" value="Ncstrn_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008710">
        <property type="entry name" value="Nicastrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21092">
        <property type="entry name" value="NICASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21092:SF0">
        <property type="entry name" value="NICASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18266">
        <property type="entry name" value="Ncstrn_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05450">
        <property type="entry name" value="Nicastrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045591710" description="Nicastrin" evidence="11">
        <location>
            <begin position="23"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="663"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Nicastrin small lobe" evidence="12">
        <location>
            <begin position="48"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI69868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="694" mass="75400" checksum="4853446A5C099338" modified="2025-10-08" version="1">MHRHTLVLSFLVVTAWSILCMGKDDMQGWKQVKGLKGAMYAEMNYGRACIKLLNSSGPQGCEAPDQEDVVAPLVHSKDLGIPYKGKRVVVVPSTEADKFLSQMLADSEMRSRVAGVLVDPTGGRPSRHSTAEKFPSAAYALYDNRSYVWNPVGTGFTRQYFGMPIYMLTASLAAETARRAAYNAHSRFKGGRHVARMVLPMQANGDSLQCIAEDTCLPVGGYSVWTAVPPLPDPTYATVPARSITLLVAQMDSNAMIHDLVKGAATSVAGLTAALAALTLLVRSGAATNYTRQLAFVALQGEGFDYMGSKRLLYEMSLNSSYVQGLRMENIDQVVEVGPIGAAWNATDNSSTFFIHSQRNPPGEYGGVDALVDAAKQAARGTQARVVNASTANPGLPPSSLTSFLRVNASISGLVLTDFDTSFKGPYYQSEYDDGLNAFQQMVEAITDAALVLARMLHSLAMPSGSSDLALNRTEAAAVARALASCTLTDDPGLNCPEARAFINPETRMYEDGTTSTAIFAYPGVMSFVSVFPKRSPNKPQVPSFIFNYLGNLTAVLPRNASATCSDDCEASLTCIGWRYNSTDKSGMGHCLNTTTSFVPAYSLRLAYNVNNSFRWSVDNSTRRSQWELNYNWPEDSAWTESNWPENTPRLMIYQQESMSTQVITLVVGLLLTAGTAAVSWIGLKLFERHLKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKB8</accession>
    <name>A0ABQ5SKB8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Adhesion regulating molecule</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_015356</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2020.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.70">
        <property type="entry name" value="Proteasomal ubiquitin receptor Rpn13/ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044868">
        <property type="entry name" value="Rpn13/ADRM1_Pru"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038633">
        <property type="entry name" value="Rpn13/ADRM1_Pru_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032368">
        <property type="entry name" value="RPN13_DEUBAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038108">
        <property type="entry name" value="RPN13_DEUBAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04683">
        <property type="entry name" value="Rpn13_ADRM1_Pru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16550">
        <property type="entry name" value="RPN13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51917">
        <property type="entry name" value="PRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pru" evidence="8">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="DEUBAD" evidence="7">
        <location>
            <begin position="226"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="326"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI70443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="37229" checksum="A686A09680222292" modified="2025-10-08" version="1">MEATLCEFRAGKLKLQSGQLIPDTRKGLVKLVQTEDTLVHMQWYERTGSGAAEVLEDDVIVFPGEATLELIPGQRAVALKFSDDRSRDMFFWLQVPKSDGDQALVATFNTRLMGAHDLGDGATEAVSLSVDGIAPTSAVEHAHVASELGAGAMLGMGQSVGQLPTAPAQTPASTASVNAQAGHLAAILGNAIAAGLGGGSRPAYGNEEQLAQSLLAQLAAVQGRNRRAPVAPGPSLAEVLRPETMLPVLRDPTVLEVLAPHLPEEHRTTEALVALAHSPQFHQQLNTFSTALQTGQLDLSQFGLRAAGYSVADFLTAIQELVAREQREGTAAPRGNAAGQQPQQGPDGGAAPMEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKH3</accession>
    <name>A0ABQ5SKH3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015367</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00102">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052387">
        <property type="entry name" value="Fibrocystin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46769:SF2">
        <property type="entry name" value="FIBROCYSTIN-L ISOFORM 2 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46769">
        <property type="entry name" value="POLYCYSTIC KIDNEY AND HEPATIC DISEASE 1 (AUTOSOMAL RECESSIVE)-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045632964" description="IPT/TIG domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="3">
        <location>
            <begin position="26"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="3">
        <location>
            <begin position="257"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1217" mass="125503" checksum="C22FC49C3D06A2BC" modified="2025-10-08" version="1" fragment="single">MNLWIFCWLALKALASWPSAECQTFVPTLKSVSQTSISLAGGTRLSIRGSLFADVSKDKVEVAVGQLPCKVLLAQTRPGVVTCETAPGLSGTYSITVSVKGSRSAPFPVGLTYSSEATPVVHEVRPSAGPPDGSLSIIGSPHFSCSSQGAGSDFNCIGDLLVGPYRCLLPYGDIEEVFKAVVPVPRWGARPFRVNCTMAGPLRNNPKLHSAVPGLGAAGLFNVTLRLEANFSGGRAEVDAGAWLYSPDGAPYMYETYPEVSAVTPSSGSLAGGTRVTIHGRGFPNLRQLPGGNTVQVTLADGVPCTIVDSMYDKITCVTTRSSALQQLSLLQQQQQELLAFGEGNTRVAIGGQYPGMRGAEYQFYPFNATLGGSSAGGGSGGSSPPSFSELWRLNTSLLVSQLEGGYGGVLMDRMEDPRRGGEDEGAAATCRRFKGFFWAPRSANYTFIVETGGYAQLNGSWIQDGQEMAGVALVNVTSSFPSPSPSPSPSTTGWSPMPVALSKDQPILLELNQCNIGAQDAPRLGVRVSSSVPRSNSIPEVQRLHVTDFPQPPTLTVRYLYGSLTALDGVRITVVAGPGGGEMLSDPRVGLQLGFGSSIKVVVPLTLDDSELAVRLAAALGLNAGGSAFSARRSVNVTAVTLEVAAAEGTVPALRGVISAAIVELPMPTPPPLLPPPPPPPALSTACRFSNPLMAPVPGSPAYGAMYGTWIGQTTLPFMSQGSLDLSKLNVTVQAPAATALNTGGGVGAWTLGKQQAGVIRRLPRFLAHNATPAEVADAVWDYTGLAVNVTLASASPMGLYSASVWQLTLPNWDSRSTAALAALVPEPASSFPAGAVLEYEFTAGTAPGSGAIRVAYGDGCECVLVNVADDPLDVIGAKLASLPGVASPLRIARTTSSNGSVEITVTFDPISNPGDREALRVVDTSGLVNDRAAAWFTTVVNGTNDQLYLPIPASMMRLAVAAPGSVRVIVNEAPAACVSPMGLCAFSFDASLTPNITYIEPLQLTYDWSQSEIPISIYGAGLAPLTDDGSSAGGTGSTGGGGTGGVTVTISGVNCSVVYVTDTLVVCNLPRDTVPAGLHSVSLLTDGKGYANGTAELITTDMAVYDISPPVISARGMTLISVSGQGFDAGSTGGDGGGNCSRLHASIGAVPCPIVSCGPDILTLLYPGAPGGVGSSGGRGVEGSPIVLQVGVPGGNGGVWFVRDWENWINNSEMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKQ8</accession>
    <name>A0ABQ5SKQ8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="145"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="304" mass="33402" checksum="EE8A4CC1DC2A17D0" modified="2025-10-08" version="1" fragment="single">KKTRVHWLHAGERPGPALTTFLQTQIQDQQTAPVALQAHSGRLVTNPAQLPQVVADFWAGVCRAPDFTTPEDCQSVLTQLAAANIRLPEEADEVVGSPTIAVSELGFALKKSQPGKAPGWDGIPTDLYRKFWPEIGHTMAALFTAIGKTGQVPTGFLDGIIKVLYKKGDPKQPGNYRPITLLCSDYRLLAKVLENRLGPALGRIISPEQSAFLPKRLIGASVLFLRHLPHLLRKLNRQGLLAFLDFAKAYDTVDRNFLMGAMEAMGAGPQLCSWTRILLSNTRARAMMNGFKSRLVKMEAGVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKT0</accession>
    <name>A0ABQ5SKT0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="151" mass="16314" checksum="9DB0CBE7237B6491" modified="2025-10-08" version="1">MCEARPTEPPGGPAAPAGDGPIHQPGETRVAVYAYDELAHGHYAHETGQGRRARLCETRSTSSCIPPLCLPRYSRSRCGGQEDVGEEVEEERCSAAWKALPRHGAVSHAEGALQARAADVGMGQQLQLPSRGPVRLLSFAIHGWQREHEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKY3</accession>
    <name>A0ABQ5SKY3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01276">
        <property type="entry name" value="PKCI_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="58"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="152"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="168" mass="18297" checksum="877386A91F955B81" modified="2025-10-08" version="1" fragment="single">GNGWMALRLIGRSATYHIPGLRRPAVTAQRRAVRAMTSAEEAAAKAAAHLADTEPPTIFDKIVAKEIPANVIYEDDEALAFRDISPQAPVHFLVIPKKRSGLTRLSKATPEHKALLGHLLYVAQLVAMKENLGDGFRVVVNDGPNGCQSVYHLHLHVMGGRQLTWPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLD3</accession>
    <name>A0ABQ5SLD3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF154">
        <property type="entry name" value="GRIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="762"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="839"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="612"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="784"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="907" mass="97863" checksum="07DB50CE4627268A" modified="2025-10-08" version="1">MRRHLVFFGQSTHLTSQFPVRVRPRSITCLAIHIDSHKELIRTTSRISETSCRLFDQLAAEHCPVVRVSLAHFPRGGLGWILRQDSDIPLPCQSHHLTEQSPPSSVSRAAALQASRASVNPNTTTSRTGLDLLQTSHVKDSGSKSSADVRSESESESGSRSRSNHGGDFAHRTLLSVPLRLCLSHEVTGCCPPALRSPALRSILSSGSSLPWEVPLAAMLLWSCRRHRVAATAATAATAATAATAATAATAAGTTAKGADADLARTTRGAAAASTSVLLSRGRDGADTDGRNGGGVDEVAGQHGDYGMYGGGAGGGGDGGFLQFWRSYRDLLPPGPQDLDTLLLFDEWELKELQDPSLAAEARAWQDVVRHAYDRWIACDTFQSEVGGKVPFSEWLWAVGCVESRAFGFRADDDGRELHAYVPFFCLANYEPGAPTCHVLRLNLPDSCRPPAVMPSVLARGKGCPPQMHDIAPCRTTDGSWEPSVDMIQIRAPNNHSTKWLKEPLQDLEDSRHRDNRGDLSEGEMRSQSGPRSVLVPEHQVFIDYGRKDNRSLLYQYGFILYGNPYDRLDWGGMVFDSSCRMRRDWVYGMAEKLVGRGFSEDHREASSPRLSTCCTGSGSNTSSGSDNPPLCTPQIDEPGTSILSYGTSGVLYGYDTACRTPGDESLGGTREGLHLLLDVNGGMPAVRRRFRSAAASIVEACGWRNLRERRLVGRESEERTLLLLSGWVEDQLRAFPTTSEEDLQLLVGWAAGGAAGVVQGPVGVGGAHHRTEEENRVPSKTFSPSPSISSSLSSSPSSPSISSSSHAFCEGACEAGGDDTWETPEYMACRGPASASRAATVMGGAEGSSRGDREAQPPPPPPPTTTTTTSETLSVLRQRLAVEYRLERKLLLQSALVLLQELKRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLD6</accession>
    <name>A0ABQ5SLD6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tubulin--tyrosine ligase-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015354</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamyl-[protein] + L-glutamate + ATP = gamma-L-glutamyl-L-glutamyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60144"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143622"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:60145"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF145">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="199"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="647"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="767"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="230"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="647"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="778"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="790"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00041448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="919" mass="98202" checksum="9F60B986E0BCB6D1" modified="2025-10-08" version="1">MVISGETHGSFLATKGAYVPQSWDLYWTVRRACHSVLGAIAPPKRVNCVPGVEAIAHKQPLVRTMQEAYGEAAFDYIPRSYMLPSQYWVWRSWTQRSSSPPDLPWVLKANDHRGRGVRVMRQKQAQRQALRGSGAQPAGDAATQTGFVLVQSYVRWQFLYDNRPSYLRLWVVVTSVRPLRAYLFRGGVLVFGDRLGSGGRGNGASATASGQGTSRQHSRRRLRLQPREGAQTQFRAASGSRNPNDRGIRGHLPQQQQQQREGFGKKPQQYEMHQVNYWTISGEKMHPWTVGQLRRHAEAEHPGDPKIFERMWAHARTSVGMVLASATRRMRTAARGLAALEGSGFEVLGVDFLLNATLHPTLVEVNALPSLARLRLAPGDPVHAGGGGSADGLSDGSKRSPIDTAPPGFTPPFDLEKERFLHHTFRLIGIPIGPPPFRGGDFNKSTSGAAAAHTAGTAASGGAPSPSVGGILRAMVALLRPPNNETAQLQAFLRLHLPLDVVAAETALPNLRSLLCPVPAAWGPAPGWPVLEAATVAVESTGSGSRRLVAAATAQAEVTACQLPELIAERRPMGWGNMERHVALGQVPKRGAGSGGRRIAWQGNAATREAEPVRLQGVQYSGSDAAAAASAKAEVMTTTLGLDGKARISLPEHDVPEETDDSPALGRRSLQSATAVEATSRGGGRALMEKSASEASGGGSGYGMAGAQMQQPCQRRCYDWDVLAAIADTEYELQQNLDFDPIFPLVIAHDLNHAALEAARRVEAAATQLSQAGDEARSSSGTGSSESGSTGSGSGSGSGSGSETFGGSALLNPQTGLQKLQTSLNFVRESITSVNMDMMRSMRYMTTTPYVLARTALPYSRIDVALSAYEAVRRREGSCAVRISVNGAAAAVESATCAVQVLQDTLEEACSGRMKPEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMW9</accession>
    <name>A0ABQ5SMW9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAST kinase leucine-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="666"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="697"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="969" mass="104020" checksum="DE6479C9CE43CBFF" modified="2025-10-08" version="1">MTLHLVGHQPMMRPTELVPASQLPVSVAQPGTRNCGQSRFLLSTIMGAQSWTELEALTHKHSSSFSHIHVSALVCRLPKLVEPAQLSKPEKSHFSRFLREVSDIVTVRLSTFDPRAIANVLWGVSKLGYSPAPPLLNKFLFEAYVRMYEFNAQELANLAWALATLAALGNRPVPMWLRKYAQAALPRTSELKPSELAHMAWALSRLFPPAAAASVTEIQLQPDGVRLSHGSLRIELPSGAQTAADRFSHCATSEEDASSGQLSSASSTSTIALFSNGSNGVSISSSSSIDIHKIPMPGSPTNPANSVVGDGPAAALGDLVIALVCQTIALLPDFRCGELVMTVTALQTLDPSAGLCLLRPALPHLLRAPPGSLSPQDLTNIWQVLGKCANESTMGVDLPYGKNGPMMAAAATGTGTGRDRRSAGKAPVPPAAAHAVVAADEQQQPEGVANSFSRPQRAAAGREKEFARPAAARLPLGATADRDQLGRHQLHALLDVTALALQGFTAQGLCLILWSWARLRVQPSDNVVQALFQRFCHVLPTQATFQCTAISLWAAAHLGLQPPPAAMELFVSCARLQAPASKAGELLALLWGLQRLRYQPSKVLLDSINARLGCLASRLSPDGLQVVLHAYAVFGASAPYPVRDRALGLATETAKELWATGTAGACGRAEPLSQPEPPLAPTVDSVAAPSSRALDVSGPMPGSSPSSSLLKQPPRVTVRYFATLAGICPGVQSADSRRRRVSTMQLQVLRWRLEANGEMRRALRAAIEGTMQGRLQELEANALCTLLATLVRLHLKPRAAFLREVHEQVSARVWAGALTHWQAAWLLASLGRLHSRPPVEVLSRLLGELGPCLSEMTDSQLLSVVEALARLRFQPSVTWMRGYFAECMTRIDRWPAASLGVFLRSLAALGLRLTGSPCRQLQVVVYRRKKKTLNAAERVANSGGIRLGPEEDLKRTLCLVCSDLSGCII</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN33</accession>
    <name>A0ABQ5SN33_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TPM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016578</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007621">
        <property type="entry name" value="TPM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35514">
        <property type="entry name" value="THYLAKOID LUMENAL 15.0 KDA PROTEIN 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35514:SF1">
        <property type="entry name" value="THYLAKOID LUMENAL 15.0 KDA PROTEIN 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04536">
        <property type="entry name" value="TPM_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPM" evidence="1">
        <location>
            <begin position="96"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="245" mass="26312" checksum="81757720154AB59D" modified="2025-10-08" version="1">MLPSTPALRASAHCHAKPFHAVSPASASQRVIRCFAVKNINNHQESNSRAGVIAKRLAHAAVAGLATFSVLTSGAWARPQGVNRPELLPKGEPTVVIDVAGFLTPSEEKRIASEVAALEQDTGFKLRVLAQNYPETPGLAIREYWGVDDNTVVFVADPSFGDILNFNVGAGIDLEVPRSFWGRLAGKYGNKFYWQENGEAASILNAVSAIDTCLREEPGRFKCSTVQGEFGEQPSSGPFGKAFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPT0</accession>
    <name>A0ABQ5SPT0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_016582</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Assembles a suppression complex (suppresome) by tethering SIRT1 and MDM2 to regulate composite modifications of p53/TP53. Confers both deacetylation-mediated functional inactivation, by SIRT1, and ubiquitination-dependent degradation, by MDM2, of p53/TP53, promoting a proliferative and cell survival behaviors. May play a role in the regulation of spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052472">
        <property type="entry name" value="MORN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="249" mass="27857" checksum="BF113671C61447E0" modified="2025-10-08" version="1">MPPNIPGTLTAKSLRSDEQLWKTSDQKAQKEGPHHTVFWTTGERYQGAWKDNKKHGKGTVIYKNSDKYEGDWAEDMRHGLGTLWLYRDGKYIVRYNGEWRHDQPNGHGTYFADNGDTYEGEWHNGKRHGKGRAVYGGRPVDGFGGDVYEGYFENDVKSGPGTMMYANGDVYEGLWASDQKNGTGTYFYMSKGKRFDGVWADGCIKCGTYSEIHTPPPGTPGALPPVELRNADKVLAEATMEAAAQASRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPU5</accession>
    <name>A0ABQ5SPU5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trafficking protein particle complex subunit 6B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14944">
        <property type="entry name" value="TRAPPC6A_Trs33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037992">
        <property type="entry name" value="TRAPPC6/Trs33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817:SF0">
        <property type="entry name" value="GEO08327P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="19771" checksum="A89A8D51289C7EF7" modified="2025-10-08" version="1">MSGLQPSPTPAAGVKKPARRCAEVCLELLCVESVRLFHEKQSGPAAAAALEAVGFRVGQQLAERYTRDKTRLGDTLEIIKFVCKEFWQALFKKQVDNLKTNHRGVYVLQDNGFRWLQRLSIPAAGSDAAREEAARQIALSYLHLPCGVVRGALCHLGVSCTVEADPKHLPSCTFTVRILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ00</accession>
    <name>A0ABQ5SQ00_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_017007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000117">
        <property type="protein sequence ID" value="GLI71701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF80">
        <property type="entry name" value="GRIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="523"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="582"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="242"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="534"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="582"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="725" mass="75870" checksum="6F58594ACE46B0D0" modified="2025-10-08" version="1" fragment="single">MMACSPKRPDPLRDAEARVRALTREVGTLREDLRRETKKRERAVAQAKENEESRRASDARCQELAYSNRKLASELDTLHETARAAAERDRRALRGLREGLAAVEEAVAARARKGSVQIQVLHDVQQQLRQLLFGSTGGAGPITGDSLPTSCAGGSTAAGSILSGAAASRAQQLLATAQMHVEQLASLLTGGDDDDTSAGGSSGGVAAASFDGREGGAMALGLMLPGSRQGSRTGGGGVDLEKEQLQEEVMRLRTELRTQEDRSGPSSFTAMELQQSLREAQAELVRLRAEMVHLNAAAAAAAREQQVTAEALDEAHRRLQQQDELHAALVTSVREQEDSNQGLVTELTARRVELEGEVSNLSQRLGTLQQQVSELLEERQGMQELLRQREEDRRSLEELLQHREEELRAAKASILEQGADIRNALSLVSRPPGHGQAATYPLPTAAAAAPPTAIAPTAAELSAALSACLALPRSASAPPPTATAVVAPKLDSALGAALAPYHNASAAIGSALAAAAAIIPPTGMPQTQPSPPLGSSAGSTGAGSWLQPATAAAANGNSVLGHIQPSPQQQLTLSSAHLTPSQVLQQLQQQRSQTQPQSQYPHKKTLHQQQTAAPVRQQQALADLTALLGLGLPHRGRVVNSGTDSVAPSARGVYPPIVGGQALGNASGVIGTQRMLPPRAAAMRDGSSGGPGAPLGDAAWRQGARRMVGGEGLLLHGTQVSSDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RL61</accession>
    <name>A0ABQ5RL61_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_000052</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI58398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="530" mass="60119" checksum="F7C7DBB09A8E6DAA" modified="2025-10-08" version="1">MAAVAAQARQFAADHVGPFLHQLQPYAWQIYRGHLYFQELLSTEHNMIPFMAWEEQLSRATGLSHSQFRFTVALFSLLLLVAGVRLFRNTTLRHLYATVIGVGIIYYPFGSGIIHVFPMAIAAYLALLLAPRRAGAIAWCTVFPYLIYLHVVNASGESWKTGNMDFTGGAMVAVLKLVSICVCRQDSFRKNKEDLTIYEAAHQLVSTPTPLEFLSYLFGLGNLLAGPFLEFSDYKEFIELKGPWSRNASRPMPKALGHGLLALLEGLWHMGVYLALTLNGWSMEHYYSTWYYSQPLVIKCAAICVIGLVYQLRYYFSWKITEASYVFAGLDFLGYDPATNEPRWGRCRNCSFLGVWLADSARNVPLNWNICTGNFLRRYVYERVTPRNRKPGFQSLLFTQLVSAVWHGLYAGYVMFFVGTAIWIFFSQILYKTEAYMPEAFQESLIYRGIKIIWTTFVLNYMACAFQLLDYKASMEAYASVHYFPFILMLIVDVLGSFIKPKRRHKPPTGPGGATAVGTNGELSRGGKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLJ0</accession>
    <name>A0ABQ5RLJ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellar associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="23501" checksum="6ECB40DB6B35FD29" modified="2025-10-08" version="1">MAPGYPAFACSQNTELSKSTDRRKHMNLLIHDYSEENKRVYGNMMPTAGSLLRPVREPVEDPRPKYQALEGEAAVSRQLASQVNNKRTPTGQFFTTINPTLYEKQAPPATYDGQNVTDTAVMTLHGSGRLSNSMGSDPRIAVDLAQTHNSIGVDNGSGGTTTLPPINGADGRRLSWLLPWQTGDRQLGKSMADAIRHKRTPIGAFANRDLAKPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN05</accession>
    <name>A0ABQ5RN05_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_000222</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56037">
        <property type="entry name" value="PheT/TilS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="14">
        <location>
            <begin position="315"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI58491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="622" mass="68655" checksum="7DABC3CEFA5E1F13" modified="2025-10-08" version="1">MPTVGVNRDKLFEKLGKVYTDEEFDHLCFEYGIELDDVTSEKEMLRKELASTVAKGGDSKLAAEAAAASEEVIYKIDIPANRYDMLCVEGIARALNIFRGTQSSPRYHLADMTGKQVLQMVVKPETALVRPFVVCAVLRGIKFDPARYNSFIDLQDKLHQNLCRQRTLVAIGTHDLSTIQGPFTYEALPPGEIKFVPLKQTREFNSKELMQFYLENDQKLKKFVPIIHSSVVYPVLYDANRVVLSLPPIINGAHSAITLNTQDVFIECTATDLTKAKVVLNTVVTMFAEYASTPFTVEPVEVVDALGNRTLYPDLSYRSLTLTTDYVNSLLGLQLPGEEIVKLLTKMQLAADLGPDGKELLVQVPPTRSDILHACDVAEDVAIAHGYNNIPKRIPATTTQGRELPLNQLTELLRAEIAMSGFTEVLTWALVSHAENFEFMRRPDDGITAVEIGNPATADFEVCRTSLLSSALKTLAANKDNALPIKLFEVSDAIVLDPSKSVGARNERRLVAVFCAKESGFEVIHGLLNRVMEVLGVPFKEDPATAGSPVTYHWSPSADPAMFPGRQAAIFTGDTQVGVFGIVHPEVLAAFEVPYPVSALELNLEPFVFNQFYESLLEPLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP72</accession>
    <name>A0ABQ5RP72_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_000938</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF55">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
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</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
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            <strain evidence="3 4">NIES-4468</strain>
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        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <end position="508"/>
        </location>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
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    <name>A0ABQ5RRK3_9CHLO</name>
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        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
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                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
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            </authorList>
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            <strain evidence="2 3">NIES-4468</strain>
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    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="2597" mass="258608" checksum="CEA1532AB197779A" modified="2025-10-08" version="1">MTATRMAPISRSPRTPITKTSQMGISKEPDGSPFGAGVDSQTEKPTPQGALSPSLFNSLQQRANKLRILLGSLHAPSGGRVVTGNHAESSPQPAAASGVSASAREAGYNTFFSDGQAGPAAQPPATVPAKGAVGTKPDLSPHQSGSIANGFAPTATANRSRPNSWSSGARTGDAGPSWQRGTQGSSQTKVQSKAKDVTSASVILREGAAEKSRSSGGGELTQASVCLRPPSQRLASVQALQHMQPSGQDAATVAAGAQRRDSSGGSPGALTLELRPPASVPAEPRVYPVPVGQKRHDEGVVCNPHVAIPMAVAGAMPTSTIYAAAAAAISTTATMAAAGTTAAANPQTVASTSHPALYRPHPPLAPPSQQPSWSSSLGEGPRRSSGGSGGGRRSSAGGSSYSDGGCQERPLHTPPLPPPAPLPAQAPQARLSRSPSPSPPTSCGGRDGLCRVGVHPVGVFLPQSSQAQHLQAAPQQQLTKLNQILPQDGSVSCALAPDGRPVAAAVTAMPPQPPPPPLPPPKDRIARGKTPGDASGLPQPLPLQVPAAAAVASVAAAPPRSQAAEELSAILSSLAAVTALPTAMPAATRQELPPPNLDRNHPDPSSVVPCELLHGMPPTEGGGHGACMNSSFGNGVAATTKSGAVCSDRAQKAHALPSAPEPQAGAVASGDGSAGRPSEASTSDPEAALVQALAVARASATAAFHRPEARVYRPRKDGFRTARSGSSSPPGMAAVTSAAPTAAGPGPGQIFAGVPAFAAPFSGRDGQAVSPRICGYHSSGVESARSNCGQRSASANSSRYERLLMLQEAGGNVGRHQGRISSGGSGAGGGGPPGPDFMRRNAVLLKKRQAWAAAAEEAEAKRRISRESEGSAGSPRNHQARPLRASWTATTDATGTVDACAASAGRGPASSTGAIHRSLVDLQRWADRREQRLAMLREEREAEERARAETQFTFKPEIGERSRRLAKLARARPSYVPLPGCSPPSRAQTTAVPAAAAAEAADPSGAASYAITRTEVGADAAGQASAAASTCPPRAQEGPLGRPPRASSAVRTRSSSANRGSVAGASSRATRGMSTSLSLGHADMLKEMRDIRPWQQQSGRAAVVKGPAQGQANAAGGGVKSKTSSAAAQLAAARNSRVRGRYATSSSTCYDDPVVGAVADAIKNATAALKLRPGPAQRATTALTTQFLSHQDPDLETRTTRPRARHKTTTYAPPQQPHQGWPSGRGARSSFGCLTGAGVGGRRRRASAIGTGAGTGCEEIHGAYAQHQQQQQQREQRRHSASLAQVDLWELASASVSPQTRSPDVHTQGGSRTLSLPPSPYPGIAIIRTSRPASPGSVGFRSNPPSPSPPIPTGTAAGGGLRLRPGGSAGLRAGLNMDSPAAAVLGSLGYGSPERKPESASTGSIARRTIDMFTSGSGGSGSGGLGVRTGWSPIYAGSTMAFGSDFSGSGMGDSLRFSLSTDSSSPFGERTAGSPVFLMLGSGAADGNATASPAAAVAATAAAAMVGPGGVLVRIPQTIQLSSPGPDGGQGRTGLRITPVGLAPPLAGDSAGGGATLIAAPTAPGGLAVGAFRYGSLTSSPATSYMALPYDGGNAVLRSSVRAGGVLQTTGFVATSTTLNDGLAQAVTAGRAISADTTDVAVNPSSATSTKFNSSGPTATPVTDIPLPSSTEATEAPPASAGAGGVAMAAVNSAFVSSSWPGVGTGARSRSPRGDRTALSSLRPEFASSCSVADTKTEPARRSSSGNGVPSALPPPQQEEVHITSASTAFTVADAMVANLSASADANAVANITSQHADSYSLARNSAPLPATSPGHTHGSLLRGVRFTKILAQQFAASLLLSNPASPSAVAAAAALGASVSASPAMAKTGGADAGPRATSSYEPASRDGILSAAGDGNEDAGGVTESLCPEPRSVSAPSGLASWLQTRVGGHVGISRAAAAAGMADYQGLCFEAVSLRTGRRPPSTSAGGAAVASTEPVSGGVVMGLVSTRSESGGTTLDATAGTTAVASVNALIGAGAHAACAAAPSSAVGGPDKRRGSDSALGGAHNAVMGILSRSTVSGSGVPSVPADTALASHRSANVATMAGTDPDHKAGQEDPLSALQSLIYKMKHLAGEGELTDCGSQSLRGTARTGSSGSGSRASDRECTGCATERCQVPVAVEGGAATMTANRISLSNGPRTAAPPHPGNSSAAGSAVTPGTEELPAGRDGGIGGSGGCTASAAVTANGSSDEASVPLAGPLPLEVLVQRAEQLRASIMGSKDMSRGCSPVSVSPAGSWTGSAMQHASPFSALGLCPGVAGAVNGVGGGPGGGVAAVIISPVQLRYGAAIANSSVHRSGLVSVSAGSGAASSSWRMGVNGLGVRSGSGYAGAEFVFPRLRSEAVPTAPTGARAIKLRPMGDSPGLAAVPQGQTMSEEEMMPEISLAGGLDQMPTGDRKSPRRSMTTALASEAAQFAPAATEQPRYSVWSLVDHLPSTVTSSTTSASAVTTDGSSTISTTAAGTGTVAAGTAPQPPLSVSQSSSSAAGLAASTGVSADAHVGYQALAPSASVRSSHSSCPEPPAQAVTSGTCLAKEGDGKQDGDSLAALQSLIRQVAHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSE2</accession>
    <name>A0ABQ5RSE2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">formate C-acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_002530</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>formate + acetyl-CoA = pyruvate + CoA</text>
            <dbReference type="Rhea" id="RHEA:11844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.54"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycyl radical enzyme (GRE) family. PFL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.54" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01678">
        <property type="entry name" value="PFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050244">
        <property type="entry name" value="Auton_GlycylRad_Cofactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005949">
        <property type="entry name" value="Form_AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019777">
        <property type="entry name" value="Form_AcTrfase_GR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001150">
        <property type="entry name" value="Gly_radical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004184">
        <property type="entry name" value="PFL_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01255">
        <property type="entry name" value="pyr_form_ly_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30191">
        <property type="entry name" value="FORMATE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30191:SF0">
        <property type="entry name" value="FORMATE ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01228">
        <property type="entry name" value="Gly_radical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02901">
        <property type="entry name" value="PFL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000379">
        <property type="entry name" value="For_Ac_trans_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00850">
        <property type="entry name" value="GLY_RADICAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51149">
        <property type="entry name" value="GLY_RADICAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51554">
        <property type="entry name" value="PFL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0556">Organic radical</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PFL" evidence="13">
        <location>
            <begin position="79"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine radical" evidence="12">
        <location>
            <begin position="718"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="modified residue" description="Glycine radical" evidence="10">
        <location>
            <position position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI60525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="842" mass="93072" checksum="5F5A1A7CD3C54C07" modified="2025-10-08" version="1">MSIVLTSPLVATICNQALPVLPIMSQMFLERRFATTTCQRGGKTHVPLHARPTIPLVRQPTLGTGAQSLRVPAFGQLRPLAASAAEPALACPHDPDQLPHLPDPRPRPAVDTGINVQKYVQENYKPYSGDATFLAGPTERTKKLWSGLEKLLCTELEKGVLDVDPSKPSTITAFGPGYIDKDLEQVVGLQTDKPLKRAIKPLGGVNMVKAALESYGFKPDEEVDKLYSTVRKTHNSGVFDAYTDEMRAARKSGILTGLPDGYGRGRIIGDYRRVALYGIDALIASKKTDVKYNLLGVMDEEKIRLREEVQEQIRALNELKEMAQSYGFDLGRPAQNSREAIQWLYFGYLGAVKEQDGAAMSLGRIDAFLDTYFERDLAAGTITEAEVQELVDHFVMKLRIVRQLRTPEYNALFAGDPTWVTCVLGGTDANGKPMVTKTSFRMLNTLYNLGPAPEPNLTILWNENLPQAFKDFCSKVSLDTSSIQYESDQLMSRLFGSDYSIACCVSAMRVGKDMQYFGARANLPKLLLYALNQGRDEVSGAQVGPKFAPVRNKDAPLDFEEVRAKLEDGMEWLASLYANTMNVIHYMHDKYDYERLQMALHDTFVRRLLAFGISGLSVVTDSLSAIKYAKVTPVYDERGLMVDFIVDGSSPKYGNDDDRADALAEWITSTFSKKLSKQHTYRNSIPTLSVLTITSNVVYGKKTGSTPDGRKKGEPFAPGANPLHGRDAHGALSSLNSVAKLPYTSCLDGISNTFSLIPQVLGKGGEAERAANLSSILDGYFVNGGHHINVNVLNRAMLMDAVEHPDKYPNLTIRVSGYAVHFARLTREQQLEVIARTFHETM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT20</accession>
    <name>A0ABQ5RT20_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RWP-RK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_002966</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription factor.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044607">
        <property type="entry name" value="RKD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003035">
        <property type="entry name" value="RWP-RK_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46373">
        <property type="entry name" value="PROTEIN RKD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46373:SF2">
        <property type="entry name" value="RWP-RK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02042">
        <property type="entry name" value="RWP-RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51519">
        <property type="entry name" value="RWP_RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RWP-RK" evidence="9">
        <location>
            <begin position="6"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="133"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="521"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="625"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="818"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="853"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1004"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1133"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1177"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1250"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1333"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1527"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1644"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1710"/>
            <end position="1879"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1065"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="250"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="341"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="372"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="388"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="625"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="636"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="690"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="736"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="857"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="873"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1133"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1275"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1338"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1351"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1446"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1527"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1571"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1598"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1646"/>
            <end position="1657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1725"/>
            <end position="1737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1738"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1759"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1786"/>
            <end position="1797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1870"/>
            <end position="1879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1879" mass="198007" checksum="57D0C820586496FA" modified="2025-10-08" version="1">MRGVRRPPRSAGRARTSQADPTLTQIREFFHLPVEEASEQLGVNKSKLKRLCRENGVSRWPQRKLQSLVALRQHIQGDDRLPPQERQALLSRLEQQWAAVLADPDRPLEREFEDMRKNLYKVRYQKRKQQRISSGSGLWIGDDGEGDEGEEEDPRNEDEEEGDDDEREDDDGDGERAAAGASVEGEKPETRVLVAATTAATAIPRRRRRSVPDEHAEEEEDETEGAVRRPRQEALPLFSGLFQRQRRRGQLAAVQRRQRRQAQASSSTSSSSSASSSSAASTPRDESGEERVQGRGDSAASEHGSRDSRRHSRSDSQYSNAPAGGSVQAREMEASPRQMHQQQQIRQRISAGSAHGAIAGVAPEAGAAASGGRERAGGQGRRDESRSSRQGSGPSSPVAGGATAADRLRPRLKLRTPTAQLDPQRLPSQQSYKQEDLEPPPLPPPLPPTQMQLIGQHSPHRSTLPVSQLGEKRLPGASGITQSWRRLETQGTTAVTTGAPGGHEMHKVMQFGNTAHPGELRARTSADRPASFTASHRASRPDSPGHSSRAGQELPGPLDQVGFATTGDREPGPARWEDQLQQRLSVQTDPDLDVGVRERNVPEAVTQQQSSGEWWQHPQAQLQQQQQLVEQPSQARLQQLPQHSLQRSQASPASLATWLPSGGGGGASEASRLSRLVNKPSRPTPYGGDQQQQLQPVPQRASQQVQPLYDEWRSQAAAAPPPQSRQELAPHIAQPRPAPLPAPAPAPSLPHSLSGFGTRVTGGDGSGTGAAFAGVASGSCSGRGETPLETMCPPSHEHDVGEGSGTLTLFPGAQPSAVKPSGIGYRGTGTRPSSAEAGAALFSSQAQTSMPLDVEYQGSSPPGPSLASAQAARPTSQLFMSQQLPSSWRPAQPVQQRMSRVPADLPNPLTTRAGSVNLTARHSSVPPAALLPLPGAVAGSSSAGGPATAPSASFAPFTAEAAMATEPDPATARSAADPAATGWYRWASTPSSDTTVRQRLAPLVTQQQQQQNTGSIGARNQGFGSGQVPAGFPSQDAVVAPPISAPAAVPGPLQLGPFSAPQTTFASQQQQQQQLQQQQQQQQQQQQLQQQLQQQQLQQAQARAQVQARQQLYKQQHATYAQQLQMQRQMQQQTHHVQQLHPQQLAARQPQVDAQSQFMQLERRRWLEEARRTEGVTPVGLAGSAGPPRFSGASRSSSTPAPGYYPGLGYPYSHTSHGEHLALSGAQLDRSGGGRESVAAEGLQLQRSGAVVGTPGGFGIPGLDDLGRGASPHAQPQWHSQPQMQPQPQLPQQHLQLLPQTVQQPLPQSQQQQQQQQPLSRRERALLALHSLEPPRAPQQQDTQLQPQTLMSGQHDQQQQRYYEDQHYQTRLQQQQQQPYSGASEGGPGTARTGSPPWTIAAAQPSLLPRSQLGMPPPSAGQGAAPSQGPGGPSQPPHHPGFTSMGVGSSAVGASRGSTMLASGVPDGDARGAGMEYGVHGGTVTNTASTSGTAMLALAPERVSSTHLGGSGSADAAFNATSVTAAATGASGTDSGPPTGTAPGGGPEAGAADPGAVIASRDGQRDHESISRGPSAAAMAMSGMMYPPSPREQPMDVAAAGQAAGAQRSGGSAPFSTALPPPLGPNGSPTSLKLLYDIWSGSPVDSSVRQGGQRSRSMPSVSQQAEGLAQGPEPVPSTAQLPSWSGLGGPDAGQGYIPTHGLFSDIMAVLGTSHEENQEAPAGALDGGAGAAAGHGAGPPPPPPPPPPPPASPPPPLLGPAVERSSGSESSATSAVQAVSRLRISPSAQPDTSSSGPHSEWHQDTVPLGGAVDSVVTEPLRTSTEWGLPGEQHLPHLPRQQQSPGPPPPAPGSIRQVGEELQRQGHHGGPSPPQHPLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUM7</accession>
    <name>A0ABQ5RUM7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase II subunit beta</fullName>
            <shortName evidence="2">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003679</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays a complex role in regulating the basal catalytic activity of the alpha subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="234"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="267" mass="30525" checksum="E9F6FB9DB0EF22BB" modified="2025-10-08" version="1">MASRQTENWRVPPPAGRQEAPDLVTDSESEESEDASGSDQEEPSWISWFCSLRGNEFFCEVDEEYIEDDFNLSGLSSQVPYYEYALDMILDNEPPHDVMLTDQQHELLESAAEMLYGLIHARYIVTARGLACMLEKFKNYEFGRCPRFYCEGQACLPVGTSDVPGQSTVKIFCPKCEDIFYPRSEYQCSIDGAYFGTTFPHLLLMTYPMFRPPKSVEQYVPRIFGFKLHPTAYGNREPAPARNNQRRIAAREANAKDGQQKQEDDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUQ9</accession>
    <name>A0ABQ5RUQ9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030791">
        <property type="entry name" value="Rotatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31691">
        <property type="entry name" value="ROTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31691:SF1">
        <property type="entry name" value="ROTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="711"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="719"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1104" mass="115858" checksum="FC8D609AE69A14C9" modified="2025-10-08" version="1" fragment="single">HGLRDPQRLFALAPLAEELLPLLLVGRDGSLTGADEPDRAKWVQLLQALSRALVANLSMARAESPPIVQAFARDAMAGPLPPVLNAPAAAVLGLATRLLAALPRELWTAEVVPAVLTDTLAAIARDEVLTALLPGVRRTLLPLLAVLRPDVTILLEVAAAGEAALAAGEAAMWQLPALHHGGELATMEWLRQLYAALPALYLPGQDSLLEGLVQGLALVCGSGDLNTQSSRDGASASPRLGPASPSAEGGAQGRGRAGICNSSSPTHQPNARAEAQWVQQSEEVFLALLSHSSPSVAMACYSLLEGLLADGRAQPGQPLLRLLARRAVVERLVVLGLSDERTRRQVARILMGVLPDVDMQSCIRPWSFWIRLYAADPQVGALAASLEASERRCRERSPSPGGRWGGPEVQDLGAGAAAPPFEWSWLRGLLLELYSADAERRRAAARHLSEDLKEANVAVFAAPHDPHVIDPFRVCLDSGVRDDLVVPAANPRLARSFRPADVSNLLAILANGELALELRRSAAEQMLALAAEERLREVLEQEASVYVITCVAALRDPATGSSLTASQVGAPGSQGLVPPTPDLLSTLDVQLPLAAVNLLFVLAAHSPKTRAWLVSERSLGAAARQAGGPDSADTGALTGANTIVAEEGRDEGDSLGGSKRGGGLEMIVGGILPMVFHSMVTVRRAVARLLAALCFGSQADRWSGWSALATGPGPRPEMTPSAAARQWAAAAPTGAGRTSSAGDVLLLPYPFQRCYGFPCRVTWVRLPPACQPAVEEEALEGKPRLQDLLSEQRHLVVLLIQERQALNAAGGDLSRLLALMNDRPDCLGGLPPAAQHTLAASVRAVAPGGLVSRSLAAVRAATSHQECTVALRELQLAVYSRQGAAALAAVDWQVHFEALLSTSPISPEDHALWVELLQPLRRALASGGMSHAQLVHLAEYLAGSALDVLSAPEAAVEPPGLPVALAGSAMLSIQPDQHSQLLCTLTVLATLVDLVRFARTRLPAHQGLRVLAALSLPKLLRTLGVAYLDNAAASYGCRAQAVQLLQEVTLLLTMAMRIGATTANVTAPAGDGSDAAATAAAAPEVLSLDVGLAEALLLCLKALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUR5</accession>
    <name>A0ABQ5RUR5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_003227</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="478"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="544"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="679"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="488"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="546"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="690"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="755"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="772" mass="84159" checksum="5897D2BEE1CB578F" modified="2025-10-08" version="1" fragment="single">MHRYQISAYGNQNKTLQAACALRRRCDGQEDMTMTKLIEICKESGLPVSPLAVTRLELNCQLFTRIGECIAEYRDVQQLHLDRNRISRIENLTSLQHLRCLYLQGNAIRTIEGLDGLSQLRLLNLSGNSITRLSGLDRLPRLEALLVSDNRLSDGPSIQHLAGCSALCELDLGHNQLGDAEAVLQVLAAMPSLQTLTLAQNPMASAQQTAALQPPPIAHKTSGATEVASTDNMSEAGRIDAAASGPAPAPPLAFYRRTVVARCQHLTALDHRAVFPQERRYAEAWWQENQQRLAGQQYKELEGSADQGPMVAGTGASLPDGAKLPTDVIYPVLTRLDPRTLAVAALSCRFWAAVAAFPLARQRLLRAQAALCDTAAAVPRLAAAIMGVRRDELDELLLTSGPPARPLVQLFYALEVLWRRYLRRRARRGRRRRQSCLAPLQQQQQQQQVEQAYLPQNHWQCQRDEAAGSEQLKAAMQQHLPAVKSVETSSPKGSRTTTSSSSNGSQAKGNEDCELEAGEGPNFNALRHSSSEFALNAQPSVVDSSMDLDSDLSSDEGGEEISDLDPEPVDLDSQTWRMANWEVALELLQHRDFPLPAMLTALPLGPRALAALRCRTGSADDLDFYIPQLRSQDVARLNASAGRLAEWLLGVEAHCRAAHEADRARTWSWPRHMTGKHEAFVSSETPQRGHGFETREADSRAVAREARDIGRVNQSQCDAARDYESQAGADQAASGGGGTGGALNGCDVPYGGARSIEKGESRDAGPEVGKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUT2</accession>
    <name>A0ABQ5RUT2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoribosylaminoimidazole carboxylase</fullName>
            <ecNumber evidence="2">4.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003196</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.21" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039476">
        <property type="entry name" value="P2CMN_synthase_LarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43064">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43064:SF1">
        <property type="entry name" value="SLL1489 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurE" evidence="4">
        <location>
            <begin position="170"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="375" mass="39142" checksum="68029C35A467D2B2" modified="2025-10-08" version="1">MLAVQPPSSVLKTRISARNAGGRRRCSGGRGRLSIEAGMGGTSSTADHQNSYTQMANGNGNGNGNGNGHASIHSQINPEFNLEQLASPSPDPHQTPEVVWGPGKSPDQIAMKLLSLAERQRVAVAARIDPATYSAVRMHAPGVEYNARARTLKLKSASAELLPKQRRLPGSVAVVSADTADQSIAEEVRVLCDYLGAYSFQIRDVGSANLHGLFANVDSLRAADVVVVVAGMDCALPPLVAGITQSPVACTLLGMDSAVLVLRVTLLPRQEITTQIRNLSHMWLVYMQIAVPTSAGFGASLGGMAPLLASLSTNTPGITLCNIDNGYGAAAMAVRMLKMASRLHSVRSAAEAAAAAAEAAARSLDAHAPPAMQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUV3</accession>
    <name>A0ABQ5RUV3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05467">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="2">
        <location>
            <begin position="87"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="611" mass="65737" checksum="2FBAA2A3D00C6830" modified="2025-10-08" version="1" fragment="single">INLPFSMALGFGSQQLSTKSVQLGSAAIQAHRHSRRCLSIGGRFGRLLDANVLDSGQSMRYRPGLVFALKTELPLVQRCVDPTDPLTHHNDFVRYRFVVPQYVTTFGQTLRVVGSLPELGGWNPYSAPSMTWSDGHQWSLECTLPQCSFEFKITVFEGQAVKWEGGSNRVVHAEDAGGDIVPVEIVVWLTCNFNATTNTQMQLAVPRGNIQDTYETGKATLEFLKLRKSKLGHDTEQDSSPEARLQYTAELARLTEAVAEQSTVVHQLSDWLGHEAATSRGASRRNGSSSTAAYTEDEENSLLLLAIDSVRVPRALRRISFVQPPWTSGLTAALSSGKESAEKSDGTEVKGAPTNSPFLRGELDSRADDIMGAAEVLLQELKQSSSPDVQQASLDWASLAQEAGQVAVALEALGGDDSPTDHALQRQLRRFAAVGCELAGGNMAAVVATEATAVSIDVDSSTQISATETGVPLVLTNLQDAVVPPSASTAVEVAASNTGGQLVPPLTSPSPSPPGPVEASPWQRLAANMKDTLGVNGPLPVVAFEQEAVQRIESNGQSQQALIEQNGTGQLEPWKPDPNPLQQEAPVGTVRGWRWPKLNFNLRAFLDSLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV83</accession>
    <name>A0ABQ5RV83_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="99" mass="9717" checksum="BEDE84E1EB4D97B3" modified="2025-10-08" version="1" fragment="single">GTLKSGSRARPRMKKSKPCCSISSGDSPAPSAAASATATVSAAMSYSCSCRTAGSRLPCASGTSTSSSAEEDTAATLAAPTRLLTILTASTPSPSRAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVT0</accession>
    <name>A0ABQ5RVT0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Dihydrolipoyl dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_003681</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="12">
        <location>
            <begin position="85"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="11">
        <location>
            <begin position="443"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="573"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI61324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="586" mass="61525" checksum="5C8D9EF304C933B1" modified="2025-10-08" version="1">MAASMAATQQRLALGAKQATCGPAAWVRPAKTTVSAKRNGAVIPDVILGCVPMLAGSRRVTASRAPQQARSAVLAQAASGDKFDYDLVIIGCGVGGHGAALHAVECGLKVAVIEGHDIGGTCVNRGCVPSKALLAASGRVREMRDKTHLAAMGVQVGAVSFDRQGIASHAKDLATTIQGNLRRSLEALGVDILIGQAKFVGNQRVRYGLPGRVDVGGEVTARDVIIATGSVPFVPPGIPIDGKTVFTSDHALKLEWLPNWIAIIGSGYIGLEFSDVYTALGTEVTFIEAVDNLMPGFDREIARLAQRLLIQGRPIDYHTGVIASKVTPGVPGVKPVVIELTDFKTKEKVDEIEVDACLVATGRAPYTNGLNLGAISAATDRRGFVPVNEKMQVLDTLGNVVPHVYCIGDANGKYMLAHAASAQGISAVENICNRPHVLNHLSIPAACFTHPEVSFVGVTQEKAEEMAKEQGFKLGVSKTSFKGNSKALAEKEGDGMAKMLYRKDTGEILGVHIIGLHAADLIHEASNAIATGQRVQDIKFNVHAHPTLSEVLDELFKGAHLDAPQNGHAASTVPVAAKPQPAVAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVY2</accession>
    <name>A0ABQ5RVY2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004288</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family. Ribosomal protein RACK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045223">
        <property type="entry name" value="RACK1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19868">
        <property type="entry name" value="RECEPTOR FOR ACTIVATED PROTEIN KINASE C RACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="11"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="60"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="146"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="190"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="290"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="318" mass="35081" checksum="1B3C0638DEDCFCDF" modified="2025-10-08" version="1">MAETLTLRATLKGHTNWVTAIATPLDPSSNTLLSASRDKTVLVWELERSESNYGYPKKALRGHSHFVQDVVISSDGQFCLTGSWDGTLRLWDLNTGTTTRRFVGHSKDVLSVAFSADNRQIVSGARDKTIKLWNTLGECKYTIGEPEGHTEWVSCVRFSPMTVNPIIVSGGWDKMVKVWNLTNCKLKNNLVGHTGYVNTVTVSPDGSLCASGGKDGVAMLWDLAEGKRLYSLDAGDVIHCLCFSPNRYWLCAATQSTIKIWDLESKSIVDDLKVEFNITSKKAQIPYCVSLAWSADGSTLFSGYTDGQIRVWAVSHSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW13</accession>
    <name>A0ABQ5RW13_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Digalactosyldiacylglycerol synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_004327</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01635">
        <property type="entry name" value="Glycosyltransferase_GTB-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044525">
        <property type="entry name" value="DGDG1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46132">
        <property type="entry name" value="DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46132:SF1">
        <property type="entry name" value="DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="409"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="473"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI61818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="55891" checksum="9C17B4BC7AB6366F" modified="2025-10-08" version="1" fragment="single">AAYLASSGDRKVTLVLPWLSKADQQRVFPADVTFETPEEQEDFVRQWARNRTGLECNFKVAFYPGRYAAEKGSILPVGDITTVIPDHEADVAVLEEPEHLNWYHHGRRWTDKFAHVVGVMHTNYLDYARREEGGQVKEALLKHINAWMCRIYCHKVIKLSDAVQPLPRQETMFVHGVSPSFLKVGQSKAQMVASGERPWSKDVYFLGKVLWAKGYTELLDRLNEHTQRTGQRIHVDVYGSGPDLKAVEDEASRRNLDLSFRGARDHADTSLQDYKVFINPSLSDVVATTTAEALAMGKFVVCAEHPSNKFFEQFPNCLIYRNPEEFSQQLHRALTSDPQPLMPHQLHSLTWEAATERFLDIAELRPGSIGPLDVALDNVLAAAHNLLTGVEGLRVVAGAGARTRDMPARITDYQPSDSDVGGLFDDKSRARRIYSKPDEKHQKGPEGGAIKPGPGALGVRPLAPVQQLARAAAAAAVASVPPSEAAGPEAAVQSSMVAAPAAPAAATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWW5</accession>
    <name>A0ABQ5RWW5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003923</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF123">
        <property type="entry name" value="PROTEIN HESO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="7"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="794"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="662"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="696"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="715"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="734"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="765"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="798"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="856" mass="92742" checksum="73C0FDE1A7354D6E" modified="2025-10-08" version="1" fragment="single">CTRMTNLSQVFESLVQAYTPTPEDYNKRVLVIQEIQRVFQGVGHVDNLRVIPYGSFVSEFYSSTSDLDLAICGTLPLQQLKPASRAEIFRGITEEQDVSLHKLDRRTKSKLLRDMGDRLLANGLASRRSMEYILHARVPIVKFVEPRTGIEVDLCLGNDSTAFKAWSVAQVASIHPAFGKLFRVVKVWAKAHSINDGASHMLNSWCLTLVVISFLQTYPDPPLLPPLHELLYESVPDMLTPRLMQGGKEPPQLVYGVMESRVKLAQEVYGSRPCKPVDELFRDFVEDGARKLRSLLAREGSFLRDTRVSAFYGKMLSSWPFSASYVLCVEDPYDDTDNTARTLGTWDGNPGTVHYITSVFERTLRHLSRHVASAAAATASRGTCSDDQIESCACSSIPSPVSSSSLGSTLVFLFGTELLTWLPDLSAELMGTELYSWARWALEQGWSAGKVHEEMLRQLGAANEFVSFESFKRRHGIKVLNEAKYATPEEKAAADAARKASERAKKAAKRAAKKVKEVAEQSARKMDVQGQEAAGRVPGAQHQVDDDCSADAAVNTALRGKQKEAGNSSRAPPSGSQRKQEPIANGASSDGAPPVSLAAKAESLLPPPPPPPPPQEPPRRRLAPDAHAVLAKALKQKLETREAGPSSEDRPGPRQASRQMSAYGAATAAIEAAACAASRRTAFESGAGARPGAQAAESTWGSGNTSTNGRGPDVAALAAAAALAAPGSGPMRSQQRQRAQWQPHQAPTRETSESEVAQEFGRLESGSNTTSTGDSVVQTALQGLVLPTQECAKVTKGTCRQQQAGVDNDTQSTRDGRQDGGRASTARSTIQRAERRRVQRQRSIASAHTEEAQVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX17</accession>
    <name>A0ABQ5RX17_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004633</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="2"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="510"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="634"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="634"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="663"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="705"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="741"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="852" mass="89637" checksum="9D6255B3B373F61D" modified="2025-10-08" version="1">MFAKLTALVGGGYSFPYAIEEPYDSAWGQWTHFRGKSKDDNSPVSIFKISSANANDPILVCARNGVKRLKMLRHPNILAFKDSCETTEKGATVIMLVTEPVKPLKDVLKELELEGQHRDEYFAMGMLHATNAISFLNNDCKLIHGNICMTAVVVTSSLDWKLSGFDLLSEHSLPPDHLLQNSSWMVDNKYKPAELARCEWEVVRQGPPWAVDAWGLGCLTQEVFSCTEMSSVENLRHTEVIPPALLQDYQKLLSSAPARRLNPSKVAQSRFLNNRLVEVVAFMENIAVKDSVEKESFFRRLPGVLPAIPAAVAVGKLLPLLASALEFGGAPPLAVSSLMVIGGHLAGDEFNKRVVPCLSKLFASTDRGLRRSLLESMDQYAQHLTTSVIEDQIYPHLQTGFTDTHAYIRELTLKSILVLAPKLANKTLVSSVLKHLSKLQVDEEPSIRANTTVLLGNIASYLGESTCRRVLPNAFTRALKDAFPPARIAGLRALTATKQYYGTDDVAMRLLPGVCPLAIDGVPEVRQAALLCLDTFVKFLKDEEELRRSKDAAAAAESAGAMGGAVTSSSGGTASYLVGQAPAVLGATSSMLTWAVSGLMNTVGGATPAPGPVSPPAPVPGPAPAAAAKPMNSTAAAAASTRGTTAPVASSAGGAKTTAAGDGWEDDDESFEVDEEEQAARSRFTTKPSTAGTSNSPRTAPPSRTPSAATSRSASGTARIAAPERNEVLGASGGGDGWNNDLDDEELWEDMDGGNKKGAKAKPAVAVLATRPVAAAAADASAATSRPAAVGTAAAARRATGTAGAARAAGEGAAAKPKLGAMKLGATKVGGSSSALGSAKDSLVDLKDSDNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX98</accession>
    <name>A0ABQ5RX98_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PLAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004595</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051223">
        <property type="entry name" value="Polycystin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877:SF194">
        <property type="entry name" value="LOCATION OF VULVA DEFECTIVE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877">
        <property type="entry name" value="POLYCYSTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="2986"/>
            <end position="3118"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="4">
        <location>
            <begin position="3606"/>
            <end position="3724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="692"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1230"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1471"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1549"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1629"/>
            <end position="1681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1760"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2157"/>
            <end position="2234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2288"/>
            <end position="2311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2358"/>
            <end position="2691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2708"/>
            <end position="2728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2860"/>
            <end position="2879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2921"/>
            <end position="2965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3137"/>
            <end position="3202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3247"/>
            <end position="3266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3340"/>
            <end position="3409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="368"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="908"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1037"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1093"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="734"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1549"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1644"/>
            <end position="1660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1668"/>
            <end position="1679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1778"/>
            <end position="1788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1791"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1813"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1910"/>
            <end position="1919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1920"/>
            <end position="1935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1960"/>
            <end position="1983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1990"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="2366"/>
            <end position="2376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="2393"/>
            <end position="2408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2445"/>
            <end position="2456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="2466"/>
            <end position="2482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2534"/>
            <end position="2553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="2589"/>
            <end position="2601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2659"/>
            <end position="2673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2932"/>
            <end position="2943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="3399"/>
            <end position="3408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3790" mass="392723" checksum="96AF4EA41EFC8185" modified="2025-10-08" version="1">MSNRNETANWRSQIAGFVEGTDSNLRSIRKDAATVPSVVPALPLGGRVSARHPEAVASARAELSTLLAAVQGSLADLRVEVAAAKRLSTNASAVVAGDMSPLAKGPTNRSSSVQPRVREQRAISEITPPMHLRTSTAVAPYGQQFGSFFTSPLGPDGLAVSPPALLAASISTPPASIASSAAPHNEELVKAIDQRLLELGHKIERSLERSMEEQSLRAQLQHAVQAAAAMQQQQQMLIRSSAPSQMFTGFGNLAAAAAAAVTGAPSTAEVPVASTAPSAMASAPLPAGNAGTGPSSLVLLAMNKADAAEKISTDVSKELQVLQKAAAKELEDTEKRLSGLQDASRQHWEKMCQISSQVESLLAQRSGVAELQAEVARVANKLVQLEINQDRSVTSIDEHQRLMRQLTAALQKLTQEADSEHAISSSRHGELAAKLEVAAARTGELEARMARVGDVEARMVRLGDVEVKLVRLGELEARMARVTDVEARTARLPDVEARLTRLADLEVKVARMGDLDAKFSRLNDVETRLARMAELEARVGRMGDVEARLARLADTEIKFARLSEIEARLARMGDVEAKMARLNDMEVKMARFTDMELRLARLGDVEAKMTRLSEVEAKLARMGDNDAQAKHIGDLKARVSSLQTSVAAVTANATASRSAQPAASPASGSDFSIQDVLRRLAALEQQVARLAAAEHSSQVQERERRRGPGTLQYDSMDDGPTSLPGPLIGLFNEVSGSPSPGTRTDSGTGALRSEQYGGGPAKDILERVRLLEDTVMQGSFGQGTSSRSMQTLLGRVERLEQIVRAENSIEAMAQEARLAVAEEFVLELRRDFQDVVGPTLEKLAAHVSELRKSQASPDSGKVATELRILSDRVDSLDAQVAEVTALERATPLAVSRVAPGGVHESSTANAMQERVTMLELQVKELNGAVEDHNSIFGRLQRHQAHMERLQHELEALRSSLTTEVTELKSRIASADGAVGQMHADVDGTRAQLEALLKLDEQLAGVSDALGSLTQRLERQEGKISIMAQQALEQRDAALAVRNVSEQLEDRVAALERRRSEQRAATEEAVEALNGRLEDQVTKFDILEQHLGWRSDSQAIVQELQERLQGQEAKQAHQEVAVKDLTGRISGQEAAVTEVRKQQQEQKMAADAAIQGISKRLDGQGAKLAEMDGLQRQQYKVTAGALQELTERLDGQEGKVTELAGQQEHQRKALEFASTAAGTGAVAISRALPPERPPTDPAAEGTGAKAGLEDVGNELRTKLEGLRGSIGDVRKELGSKVQVLEAQVQELLAFREEILSSYVELQDGVDRASNAADDAMRKAESHGEKLQAISRQIDSQGSATIDDLNAKVSELQQRVSDDVAAQQGHLEALEAKVHALSALVTAASQDSQAAAEAHSRAEELAYELSQAQESLQSLVLDSGHLAEQLARVGSSGEKTAERLDRIAADVHGHGTAIAQMLDGLRRVESRTVATPQAMGSRSPAPLPPRPVQDSAAEGMARLGQMAGGEAPDNGSRLSMTLPATRSGASQAILNPLYSASSEDNVFGSIMPSTSLGSSQPSGADVRTGSIGSPERPAAPLQQQQQHTPGGTARQRPILEPLFSVPPPGISGEATVRDKAQFYEQLASKTATENAGVVPKRARAGSQSSAEAVSSQESTVAGPRPPRVPGAGGANGAGSGGPFAASSGMLSDLVNDAEPMSPGAAATTAAGAVGLAGLRHAAQISGSGRSLPQTPTGGVAQPTSPLAAAGALAAVRAARSGGISGDLSSAPKDEGEEEPGASPLAAGGASHVFGREQTFRRGVADEEVAISSSDDEQRREDRSGLFNRERTFRRGEADEEVSINSDSDGSDEDALEAPTPHMQLATTQGFDDDDDLDMVGLAPPRATFVPDTVPPAQSVRAEPEPAEAGSARAKDRQDDVPGSRPSSSSSSSRSSDSNDIDEDIADESGVNQPAFDDDDDISSAWPGPAASAAAASHSPSARSGPTADEVQPVAATAIGPTPAPAVAPQGVIPGAAGAAAPVHTSIPSAAAAATLTAEENPSQLSTSGAFGGPQLAPLQLSSSAAAMFSLSGDNLSRRKLPPLGALPPLGAAGAGGSPLAVNPALSNSRGLDVVYRAPLVTLQNSGNLSGYLSGDLQTSLGELPPVRTLAPLPPLQRAGALPPVGSPSGGAGVKGTNAIDSPDREDLSKQAEQLPVEAPLSDTPFVYHPVEPGSPLRNLLSRPVQQPHPSESVDHEVPIPAIPESHTDTSGSMPYVDNIGDLSGLSQEPSTATSFIVVPLAHTHKEQLPDGANVASEGDGTTLPPANTGPGGRNLAALLDSDSDSFLREHSSGAADSEVGVIASGGGFAAVANVASGEHASISAEDGTPPPLPFPPLSPVGDRAKALPPLGRPGALPPLSRPGALPPLGSPLPGSSPSGHAPQPAEHAASALSPVSVGGSNGAAVSPGGATTTAQPTEEPADSRAGDGDETEEVAEEYDDDFDDGANSSAPRGVSPAVTKAPSAASIMVAPPLDDGNYNPSETELELPAASAGHGSLNASDSLSSLAPPLPGISSQGSAFDSSGVPASALPPLPPGVRRTLDPVVAPPAASTASRMSLLTSEDSLALIAPPEGVGGGLLEEGSDEGDDIFAGTDPEIPGEPGRRSARPSLGSLGPMQQQRRRQERESASGRRKSSEAGALTIEPSNTSLEFNGELSIEAERVVAMLSSAQPSAGGATSTGSATSIGGTTSMGAPISMGGATSMGGPVSIGGATSAGGATSIGGTTTSYGGTASDLSVALPQRRFENSEEFITSPPSIPLTGLRSIYAPRPGIADTAAAVGGLSTGGGNPGGVRSIYASTAAVAAAVNDDSRVQGSLDFYDHEEEVSSSGEDVGGDNEGVDDAVGRQRGMAVAYGDAGARHGYTAAAEGMSGLSRTYSDDSMELEVFRPPPPAAPQQLPSVAPGSNRNRRRSSESNTSASSLRVHEQEQRAVPAIPFGHSLGDMSTSSGAYRVQVATGTRPGAGCSKGAVRLTLYGSEGTHVAQLRPAARSARHDPSGDQNTDEDAYFREGATNDFMVDTAGDLGDLSAIYIWHQGEGFSMSWYVETITVEQLMTGLEWIFKVQDWVRGGQRGGRVVRLPGANLSLSRENSLARLAGAGLMGSQPASPRPGISAATGPGASRRLGTAGSGGAPQRRGLAAMGDESMDVSSDMPSPVDVGGSSDGRKGVGSSIDALLRGVQAGRHNLEAAASASTARIGIEQSVGNAMTTSGSVNFSDGDDGDDLLPGMGTGKGGSLAAAATAAGTPKSVFAGATTAATAAVRDAARSRVAANVPQGIGVVLEEDIEEVDGLMSTSAAFSLGPVSSRSGPALVSPRPPLPPGAPRRRDSLADDEEGSAELGNAGGGTSVARRLPLPPTPPMNRRNTGCNSFSGGRRLSASALEAESSMESLTGVGIGGGINSRRRVRISASGDDGGELTSELSVEGSELSIASAASLSLFAPEGVRPPPLAGAIGVDYMGTSRKRVSWAEYAEEFEFLRDEASSSGTVATLTEEEVLAAQGETVRPDAASVGYDELIRGANTLGNGAAAAGSSNGGASEPPLSAPCGAADGTAFTEVPLKQRRGKHANSYGYKIKIFTADKLNAGLGNSTVHLELLGREATVAQALPKERGTFQRGSVDRFTLYSTAGDMGQLLALKIWHEGKTLGPGNWGFEQVVIDDKLKGNRYMFRNPDPECMLRKGRRSCLVLKPSVSRIDESEDLAEVAREYETQLSANRDLSESERAGLRGKLEKINQRLSKLGMKLARSLSGSFGFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXB4</accession>
    <name>A0ABQ5RXB4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="196" mass="21512" checksum="D2AE7F79314FB2C5" modified="2025-10-08" version="1">MGYRLRFDGCVDAPRPANLPPTGGLFSQSCMCTRALVNVRRYYSRQTRPTPAHMLPLQTDQHASLAAVGAPGKSSAADQRPPYRNQGPQLQMDRAHSQAAHTDAAAVSTVSAVDRWIVQREGLGVELRIMAVAASSRRSAPRLLARMQRAADGIETPASRADEATHMGRRALAPLPPCYRQPVPCCLHKHHRIHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXR9</accession>
    <name>A0ABQ5RXR9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glucosamine inositolphosphorylceramide transferase 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004984</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012444">
        <property type="entry name" value="DUF1647"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056442">
        <property type="entry name" value="GINT1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31389">
        <property type="entry name" value="LD39211P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31389:SF4">
        <property type="entry name" value="LD39211P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07801">
        <property type="entry name" value="DUF1647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24793">
        <property type="entry name" value="GINT1_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="531"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosamine inositolphosphorylceramide transferase 1 N-terminal" evidence="2">
        <location>
            <begin position="159"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosamine inositolphosphorylceramide transferase 1 N-terminal" evidence="2">
        <location>
            <begin position="270"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="986" mass="108673" checksum="82F0773D1F8522F3" modified="2025-10-08" version="1">MDVGGEQAGKLQLWTKQRFNTSILVYIVISLGLALVLFIDPLVDKPSGLVKLQWFSRKPLSHGLDACSWDYEGSWKIGFAKGAPDPLSLNISSEPVLTCANLVNRTAVSFVADPFLFVPPVNSVPKSLLATFLFGNRGGDGVGVGGNVGSGDAATATPWFAFYEMKNLKRYIGELGVAVSYNGGASWHHLGTALSEPFHLSFPHVLYDNVTGQYLMFAETSKAHDGAIRVYGTSLLKFPFGWELLERRYPDDRGWPATRRWFQFGAPAKYVDTAPVWFENRWWIFTTRVGSPGARQPKYILLLYTADALLGEWRPHPSNFDGAAASMALGGRVPFGIDANPRTARNGGRPFVYNGSLYRWAQDCSRYYGEALLLMRADVVNDSLYEEQVAARYEPRRDAMSWNAERLHHADVQRMPDGSWVGLVDGDRYGDGMAHFVAREQWFAELKALLLRLVVLQLVLVGLAVALLYGPPFVQSGWRVTTYGPYISSLLSRIVLPAKGRFTLSSAFLSRNAGGGNGGFSGCNSPMARQLAVVATKVAVAVAVSVGVMAFFPRMVYCPRWPISVPAFPSSPHYVPDMPYVDPSAPYNVTNLTVVTGCSATFMDRLENLVASLQYWAPNTRVVVYDIGFTSDQLGEIRCWSGIEIRRFPWEKYPSHVRDLRTYAFKGLTFELALKEVSNVVLWLDCGLELRGPLTPVAETLAANGHVSVQQSSTPGRQGYPFGFMAEKYVRRFFNFSDAEFQRIKDMPYCAGGFQGFVRGSAAHKRILDPLVACSLDSESCIAPPGHNRGQHCYDQTASTLLIRHNNFSTCLPRELYATSSTRKVSYDPRSTSSPIVVASRRYRQPKPYRGLLRRHWNCSSDPSGNPWRTIASEHTESTISHASLSKTMGDYAGALADFAVQSASCLGLHVAIQLVFWMQVLFWVSQIRFPLLSNDGMPRRSGGAHTHLVASACTTAAPSLGLVVAASATMAMVSLAVVAFTGQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY28</accession>
    <name>A0ABQ5RY28_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004894</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05286">
        <property type="entry name" value="QOR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047618">
        <property type="entry name" value="QOR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF13">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="3">
        <location>
            <begin position="83"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="400" mass="42480" checksum="BAB629D3B0AED229" modified="2025-10-08" version="1">MYTSCKNNFEKLSPLRNSDLDTCSRLRMLAAIRSKSIKLLLSQRLLNSCFSRTATGLHGFTLSAMTTRAIPATMQAIQVSEVGGAEKLLLARDVPVALPTPRTVLVRNHFAGVNFIDTYHRSGLYPVSLSPFTPGVEGAGIVVAVGPDVAGWKPGDRVAYITRPPTGSYAQYTVVPDGKLARIPPEMDFEAAAASMIQGMTAMSLVTLAYPVQKGDAVLVTAAAGGTGRLVAQLAAAAGAHVIGAASTREKAEVAREAGCSDVILYGEQDLASEVMRITEGRGVAAVYDGVGRATFDAGLACLQKLGFMISFGNASGKVPDFDILRLSRNNVRLMRTALWDFISTPEEFESLSSRTLAAVTGGTLKLLITGRYSLADALVAHQDLEGRRTTGKLMLQVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RY40</accession>
    <name>A0ABQ5RY40_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4-hydroxy-4-methyl-2-oxoglutarate aldolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004538</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16841">
        <property type="entry name" value="RraA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.40">
        <property type="entry name" value="Ribonuclease E inhibitor RraA/RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005493">
        <property type="entry name" value="RraA/RraA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036704">
        <property type="entry name" value="RraA/RraA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33254">
        <property type="entry name" value="4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33254:SF4">
        <property type="entry name" value="4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03737">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89562">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="13955" checksum="B6DA41BEFFBE15C3" modified="2025-10-08" version="1" fragment="single">MQLAFTQRFVLCNISLLKHASGLSCATARAMSSSITPPPDADRKGTADLTDKYLPDPVDTICTRSVQIVIPNLFRDFGGKLRFSGQATTVKCYENNPLVRKALEEPGLGRVLVVDGGGSLRCALLGDMLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYX2</accession>
    <name>A0ABQ5RYX2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyanobacterial aminoacyl-tRNA synthetase CAAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005572</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025564">
        <property type="entry name" value="CAAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033344">
        <property type="entry name" value="CURT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33222">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33222:SF4">
        <property type="entry name" value="PROTEIN CURVATURE THYLAKOID 1A, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14159">
        <property type="entry name" value="CAAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyanobacterial aminoacyl-tRNA synthetase CAAD" evidence="2">
        <location>
            <begin position="58"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="143" mass="15657" checksum="0118A90DB2B049E2" modified="2025-10-08" version="1">MMLAAKPSVSICKVTGRRLVSGTRAVRPTVGRCQRLVVRAEETTTSTPSFDSEKVLKDLQEKWDAVENKGAVAAYAAGTVVALWLSSTIVNAINAVPLLPKFMELVGLGYSAWFTYRYLLFKSSREELLKDIGDLSKKISGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZB5</accession>
    <name>A0ABQ5RZB5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_005126</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GOT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF9">
        <property type="entry name" value="GOLGI TRANSPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="16889" checksum="92BC8827D51F40F2" modified="2025-10-08" version="1">MLSSGWLTDQRKIGLGLTAFGILFTILGMLLLFDRGLIAMGNLLFLAGLTTTIGLHSTVSFFMKKKNRKGSVFYLGGCAVVVYGWTVVGLALEAYGFWLLFCEFFPTVLQFLRRVPVLSKVLDLPVLKLVFNRLQQLGGLPTTQYQAGIYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZQ0</accession>
    <name>A0ABQ5RZQ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005470</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF35">
        <property type="entry name" value="PROTEIN O-GLUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046187466" description="Glycosyl transferase CAP10 domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="4">
        <location>
            <begin position="162"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="475" mass="53760" checksum="BCB3AA1EAF282540" modified="2025-10-08" version="1">MRPWACPLAAILFLGGAAQLIATYKPHTASDFALRSLKSDDEDVGATNTGALDSAGQSTPMGTDIERPFNYTTLDCSRYEPTFIQIARDLQYSRKLGISLNSTLYMSARFGSVHMRGLTVGFFGGTAYLLTPTDFRNLSHHAPLHLAYMRMMLHLESSFGAALPDVAFVLTTSDTPRYVSPLLVNSTSPLSELRSYEPGFVPGPYPVCGIGKSDFFPDFLLVPNFHFHMKLYDQTFLAAIPEFNKMSWRDRKPVLFGRFSRYRTNRVSEDIFTYKAGPEGRRICYKGNRTCPFRDFFINKIVPKDPARMDVSFIGRVSMLRHSHYKYLAHLDGQGISSRLEQLLSLGSLVFKEASGYYAYYYRSLLQHRVSLVEFWRDEEVDGPEDVLRELDWAEANDEEAARVAAEGQRIAATYLTGNGRTCYWYRLMHGLRSVLSYTPLLSQWPEAQPLRLVVEQQLLATPGGKEVYDAPWAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S026</accession>
    <name>A0ABQ5S026_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36730:SF1">
        <property type="entry name" value="CATHEPSIN PROPEPTIDE INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36730">
        <property type="entry name" value="OS03G0210700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="183" mass="19542" checksum="6AB2FA7717274C45" modified="2025-10-08" version="1">MPSLFPTKPCGGALTFCCRWGPVRVQPAPAGHVSPGATAAAILAATAFLIGSPAAYAVGIESVDILPSSFEKPAALSKYADEQKQKLADADEAFRNSGTLKELLARSEANKDKNRRDVQNKYCYRQAELGVGDCGGLRYIPGLTDSGKQKTPQWLADLLGVEVPDSDPAEGKTLKELIYDDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S055</accession>
    <name>A0ABQ5S055_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Glycylpeptide N-tetradecanoyltransferase</fullName>
            <ecNumber evidence="2 5">2.3.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NMT1</name>
        <name evidence="9" type="ORF">VaNZ11_005679</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N-terminal glycyl-[protein] + tetradecanoyl-CoA = N-tetradecanoylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15521"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12667"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133050"/>
            <dbReference type="EC" id="2.3.1.97"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the NMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.97" evidence="2 5"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000903">
        <property type="entry name" value="NMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022677">
        <property type="entry name" value="NMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022678">
        <property type="entry name" value="NMT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022676">
        <property type="entry name" value="NMT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377:SF5">
        <property type="entry name" value="GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377">
        <property type="entry name" value="N-MYRISTOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01233">
        <property type="entry name" value="NMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02799">
        <property type="entry name" value="NMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015892">
        <property type="entry name" value="N-myristl_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00975">
        <property type="entry name" value="NMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00976">
        <property type="entry name" value="NMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase N-terminal" evidence="7">
        <location>
            <begin position="47"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase C-terminal" evidence="8">
        <location>
            <begin position="220"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI62888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="413" mass="47130" checksum="3CD437DD398731A5" modified="2025-10-08" version="1">MEGFSSAEVGRKQCPVDYKRQAKDKYAFWETQPVAQFREGSEGVEGPIDAPKTVDDVRSEPYYLPESFEWSICNLNDDAVAEEVYELLSNNYVEDDDSMFRFKYSANFLKWCLLCPGHRLEWLVGVRVKASQKLVGFISAIPAHIRVRDNTVAMVEINFLCVHKKLRAKRLAPLLIKEITRRVNKCNIWQAAYTAGVLLPKPVATCRYYHRSLNPRKLIDVGFSRLAPRMTMARTIKLYKLPETTFTPGLRECRPDDVQQVTKLLNKHLGVYKLAPKFMPEEAAHWFQNIDDVINTFVVESPSTPGHVTDLVSFYTLPSSILGHPQHTELKAAYLYYVVVSSVPLRQLVFDALVLAATRGYDVFNALDLMQNETFLRELKFGQGDGQLHYYLYNWRLGGGCSISPQDVGLVLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0A2</accession>
    <name>A0ABQ5S0A2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADP-dependent malic enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MME6</name>
        <name evidence="4" type="ORF">VaNZ11_005710</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the malic enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF90">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="3">
        <location>
            <begin position="135"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="2">
        <location>
            <begin position="327"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="615" mass="68209" checksum="0796F0EED471282C" modified="2025-10-08" version="1">MLSWTIDTGGCFVRCMLGCLRRSKPFHHAACASGETVVKAVSQYATGSSFHEEDDKAGRPTTPWVRQVISGVDLMRHPKYNKGLAFSDTERDRLYLRGLLPPAVLSQELQLERTLLNIRSKDSDLEKSTYLQGLQERNERLFHRVLLENLEELLPVVYNPTVRLACQKYGLMFKSLPRGLFITLEDRGRVFRILKNWPERNVKLLVVSDGERVGDLGDLGVQAVGVPISKISLYTACGGIPPSITMPVMIDVGTDNEELLRSPFYVGVRHRRVRGDPYYELMDEFLTAVKRRFGNTVLVQFEEMAYDNASKLLNMYRTEFPCFNDDMTGVAAAVLAGIMAALPRTGGRLSDHRIVFVGDGAVVACIAELFATAVATQTNQTVLDARRRLWLVDSQGLVTRERGDSSTVESYKLPYCHSGPDCRDLISAVKLLKPTVLVGLSTHGTPPFPFTPEVLQTMSETCKHPLIFPLSAAMPECTAADGYSFTEGRALLATEQKVEAATLPNGRTSTPSQCTSTYIFPGVALGALISRCTKLRDEQFIAAADAVSRMVTEEELSQGSIYPALSKIRDVSASVAKAVAQKAYESGFATALPKPHNLYEAARQNMYHPQYRRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0J5</accession>
    <name>A0ABQ5S0J5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nocturnin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006371</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF45">
        <property type="entry name" value="NOCTURNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="428"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="439"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="608" mass="65819" checksum="FA1EFB03DEC56A78" modified="2025-10-08" version="1">MVQWKEFSSRQRLPDAQARLGSRSLTALRPPWLRPVPPGGWCKRVRMTSLECSAEPHGAIAAVSTRSSKYKIKRTPVILNYPRKPDVAHFRVLQWNVLADGLAQNGDFCRAPPGCLEWDYRRPLLLQEIMEANADIICLQELNHFDELSQVLSSLGYDGAFREKHASPALKYEFPPDGMAVFFRRNRFRCCANGVEGRSFHDDWTGREQSQGYLQILLYDMMVGRELLIVTTHLKAKDGAECEDVRLRQAGQLLRNVEVTLQRLQNGTAVDATAVRNGNNDASYSYCCNGNGNRNKAGSSSVGGNSAAIGRGNGTASAADAGAGSHYNCNGEVGNNCSNGNSNGGHGSATTGAIVTEAGSCSTHRDGVTAGSGASASSIVNNVSQFLVPVVITGDFNTMPGSKTCEAFREHPLCLLSLWEQRPLDATLSSQSGSDMELPPQMQQQQQPRCPGPGNVGAVATATVSDMTLTSGVSTQRQQQQQQQQTPPVATAALLSGSETGSSLCSRDAEGDVASTGRSEFSTWKFRVKGESKRISDYIWFSGRGKGGTGGPLRPLQRWRMLTEEEIGPGALPSAAYGSDHISLCCEFEWDVDADLEWVPMRGEADWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0J7</accession>
    <name>A0ABQ5S0J7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006388</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="10"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="486" mass="51121" checksum="226C4FACF80E8B53" modified="2025-10-08" version="1" fragment="single">MDHKSPHTQTSLPAGWTCAFDHGTGHWYYYNRSTGATSWTPPQPDGMITSVPQTSAVPTAAVTASSATTAATTTTTATQALVSVKADPVDTGPKTFTGAGITAEGRATGSGWYYRDRGGVLHGPFQQEHLRAWRAFLPMDLLVWYAEIQPPRQQLQDDEQFARGRGETLTRDAEVREGCPRDQQQQQQREAGMKEEGLNGADVVGEGAGAEGSGGNAGGGGGSRFESDLATAVRNRALQRDGVARTLGISDYGDAEEADDGTEEEHDRAGGDEGAVVSNPKPPAKRVRHESTFGGSGGERTTHGSERLPSRSPSPCAPVVAEGEPDGPVGRVNGVEPSSAEEAIACGLPGIELAELLGDGQLLADWRQRFPGNRPPGTAPPASVHDHWLHAAAAAAAAAHAYGGGAHHPHYGTTHAHYGQQQHYAQTAAENDGDAVRRPRTREESMLEYAEAVLAGLPPDDEAVVLARQAAAAGKSLAEVVNFSWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0M0</accession>
    <name>A0ABQ5S0M0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_005560</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF91">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1573"/>
            <end position="1594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1606"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1647"/>
            <end position="1673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1685"/>
            <end position="1706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1713"/>
            <end position="1733"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="577"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="756"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="966"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1096"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1350"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1464"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2093"/>
            <end position="2118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="756"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="771"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="967"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1098"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1417"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="2102"/>
            <end position="2118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI62811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2118" mass="225684" checksum="7426B9A33CC28AE1" modified="2025-10-08" version="1">MPTGRNKENWPTYEAASRAAWCIALFALSVSSSATSIMVDGSPCVSHGVCPSGTFCTRTSFPWLTSGSATCVSCWQCCLFPDVYGAESCSSHCRCKRSQSCSLDSDCGQGEFCAVVLSRSDLPVCQPCHLCRNDAQAWCGACATACPSAALDDNGVAPQLQQGVGLEVHFDDYYVFAAFDISGHPLAQAGFIEASAQSGGLVQVSEAAVRMWLADLPTYVQDALLQPATSQLLVSSPTVGGATASRIVSLRDIAARLATVAARRDLLCPQLQSVGGTTGANSLIATTTVPEGCICNATANATTFRCPGGQRCSRRAWLPLPADVITLGGTALLKARCVACEPGSYCPEGTFVEEQDGLDFMESVYCPKGFYCPTPALKHQCPAGYYCPTRSLVNTSCDYQTLLLTPSPQEDAQGTAVNQTMNSYQNRQDILQRMADAQMPLKGSYCPAGSSTPDELCRAGFFCPDPAQELICPSGHYCRAESIKPWVCPHLMLCPAGSTAPRVWPAAVVTFCGLALSIIIVGYGVSNMYDMKVQSSLLNTRDQDNRAAAARTLRKFVARFHNGQLSRTARKMWHVERHVLDLCKLKWTGPGKARKSSAAVLECVNGKFEDAQLSAILGPSGCGKSSLLALLAGRDTHWPLSDGEIFINGVKAMSPRDLNYVTGFVPQDDILYADLTVEEMITFSAALRLPDAPSRGPSVTATANTGLAEVDTNTWATSPGFTAPGLLSEYSGSASGYSNTTAPLYQATGSINSGTAGGGDTVDGGGNNASRGRDNGSRSRGAKSDSGKYTPAEVRRILIADVLQMLDLSMLRDQRVGSVSDKSLSGGQRKRVNIGVELVARPAVLLLDEPTSGLDAACSSDVLISLSDVAKDHGVNVIMAVHQPRRTVYALFDKLMLLDRHGRTVYQGDPTMALQYFRSLDYKYLLMDENVPDTLLDIVTGKYYSERYESDDLPVMMEPHTVTSDTAPAAAAAAASGSGDGPNAAPSDVGVPAATDVSTSPAGASAALASTVRNGLPSSGLTAALGSNIRTPELSEGLVKVLRMEFDHILHERGAAKRGHKLNRQQLRRLFKSFGLKGPETDDFVQGILEEAIRLRQRQQQQAQPPSPQQVEPLQQQPQQQQADPDCAGHGMRPYDLKLVHTPFAMCQQQFQQQQQQQQQQQQQQQQQHNMFPAWSGLSLAAETVRPPVDREQAVSLKELLAVLQLVLDDKLKVEHLQQHVIKQDSKIVRKSWRYSFHQIREPPHVDDTLGTPIAARVSGRASSLWQMQSSARPCKDRQKPTDAASEVQALSLPPIDERTAMSAISPADVDTGHTRLAMSRLRAKKYAAAGEGGGEGGGGGRRRIIWTGSASKGNVKPPNSLDCNHHQQQQQQSAVAVRRRLTPITQLGARLPQQDSERTMMPTTQFSPSPAVAAEPQTTTSTAGDDNNNSSVYRNISPCVETTVGTAAPRAYPRVVVGGNRNNSLSRPVAVGQKGSGGDIEQSHQETGYLRTESAAAGIAKVLSLAKSKRVSTAAVPTENNKASTAPLAAVGAVSSSSISWFRRMKTERLHASWLSQFITCTRRAELQSARMFWSSSMLEAGLLMAAAAAVGLSQGATDWGPTDVPGRIIMAMMCLAVLAVVQHLHSFSANRLVLQRERGTGLDVTAYFVARCVADIPWLLTAPVYFSLPYYILTVPRASYLPYYYVVSLGVWWWASGLSYWVTVLPLIPPAAAPTAAVLLTLISGALLSGWSSPTLVDSRGTIVEVVLGLSYNRWAVEALTISELEEYMDSHSNIIAAMYGKKGICRFDVALPPGADGVSFGSVANNDAKNMTVMREAVYRWITTTGSGGRGGIDISTSTSTTTSSTIFIAGFCSETRRSDLITLFFMGLGLRLMACLTIKLDAQLVTTLLTIRQWLGQARAAVTASCCCWWRWCCRRTGCTLWRWWFCLGMCANLAVTAGDEDNKENEENGDNGENKENPVTAAAEASLATAADSGVVVVVGDSGGCGDSGVGVGDSRGVGGGDSGVVGGGGAEVVRAQGDCYGGKAGAAAEGATENQCSTSGGHGAVRGSAAAEYPDAAVLGGQTALSSPAADILCCIREGDGVSQDHHLGDLFPASSPATLPTTASGSVLQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0S3</accession>
    <name>A0ABQ5S0S3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-ascorbate peroxidase</fullName>
            <ecNumber evidence="3">1.11.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006149</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.11" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00691">
        <property type="entry name" value="ascorbate_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044831">
        <property type="entry name" value="Ccp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002207">
        <property type="entry name" value="Peroxidase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356:SF66">
        <property type="entry name" value="CATALASE-PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00459">
        <property type="entry name" value="ASPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="6">
        <location>
            <begin position="145"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="232"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="356" mass="38867" checksum="8CF791C5813C3E0D" modified="2025-10-08" version="1" fragment="single">DILSAYQSLLIVPIYPIFKNFCLTRASIVTMQKYMSKSTAQRASIGSSRQRLSTVRVKAAVNVEQLKALRQELFTYINSRGCNPIIVRLGWHDSGTYDKNIADWPARGGATASIRFKPEIDHDANKGLAIALGILRPIKSKYPDVSYADLFQMASAVAVEAAGGPKIPMQYGRKDATSPEQCVPDGRLPGAAHPFADGSANPAEHLRRVFGRMGLNDKEIVVLSGGHTLGRARPERSGFGAEKTKYTNVGPGKSSASPSGSPDRPVTPKPAGQLGTSWTVNWLEFDNSYFKEVKAKRDAELLVLPTDACLFEDDGFRPFAEKYAADQEAFFADYAQAHQKLSELGVEWEEGAPVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0X8</accession>
    <name>A0ABQ5S0X8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase family 42 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_006523</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003476">
        <property type="entry name" value="Glyco_hydro_42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013529">
        <property type="entry name" value="Glyco_hydro_42_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36447">
        <property type="entry name" value="BETA-GALACTOSIDASE GANA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36447:SF1">
        <property type="entry name" value="BETA-GALACTOSIDASE GANA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02449">
        <property type="entry name" value="Glyco_hydro_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 42 N-terminal" evidence="4">
        <location>
            <begin position="109"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="598" mass="66562" checksum="41E68A5C72F79076" modified="2025-10-08" version="1">MPLLNTPKRRNSRGICQSPILLTGSFIVTLAVMLLVLSQSYLSPSRLSRLKSKFHLTDFHVVEKLGLQKQHVRMERFATLFHLELAERFNLTQGRDNTRLDELVATLLSMESVGITTVVFSSPWEFLQSTDGPVNFDYLDLLVSSACQNTRLKVAFIFDLVRAPQWVFERWPAAKAADSHNRQYNLLSWFHPEAYQLALEVLKMVVTHLAMSHAGCVSSVQPAFNNEYEAKYTQEHDCFQDYSTPALTAYRQWLQARQPQLADLNKRWGTNVQYWDDVAPPVLEAGSVMAPDLKTRYWDFLKFRELEGAKVLSRACSVVQSAGIQCFHHVPELFTVLVAVYGITMFKYIASDPHTDYIVLDASFSTPYGSPVHPAKLRLSVAAAVGYGKPVHLGAAVQDSIDYDLLSSAFRNALLAGAAGVGITNWLPYIPLNPSLKETLHPSLAPGGQEDGGGSTAACSQGVVSVQVGLFIHFESCAAWHGFQWSSDNKDPVHDLIEAAAKKLTFDCAPRLSVFLEFDRLMAALPSLDRVLFVEPLVVWGGKEFKSYTEVKAAIEKMPHEVLRFPGIEETEAAELTVLHELLVHEVPPVGASVRLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S191</accession>
    <name>A0ABQ5S191_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16306">
        <property type="entry name" value="TRANSLIN-ASSOCIATED FACTOR X-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16306:SF0">
        <property type="entry name" value="TRANSLIN-ASSOCIATED FACTOR X-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="239"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="387"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="796"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1218"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1280"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1336"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1814"/>
            <end position="1891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1939"/>
            <end position="1978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2140"/>
            <end position="2164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2254"/>
            <end position="2288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="963"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1471"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="390"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1226"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1345"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1821"/>
            <end position="1833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1834"/>
            <end position="1878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1939"/>
            <end position="1954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1963"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2277"/>
            <end position="2288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2288" mass="236439" checksum="91EC99FB0EB6AFA8" modified="2025-10-08" version="1">MEDARGSWRGSTRLGNAVGSEAQQAALYSFQAAQQLDLAPGYQKGKRPKLTSLSLKSINCPPGVSPGPIPLISPRPGSNSSIGQTTVSAPHLHLPAEFSRLCLEVDGEPDKGASVLDLEASFSDSQGRFQQWDLVGAHDAGSAGTAPELPAVPALAMPAGAGHSIHRSPLRRSTLSPRTWNEEDGQQQHVLLSTGARAYTTSCEGSQEAGMPLWPKVVLTSLNRPAVGSSAAAAAAAATAAGGDHQQQQQASSRYSNGSSSNAVHNHNYSSDSNKNVNKTNRSYDGVEHQQLEQQQVGVPQPPPPADIGLHGSMFQQVRTPNNESTALIISPSQRAFRHEKVRRHTSLDLMNLPTQLLVSSSRMAPTPRKPASAVWAGQPWAQDEDMTTVHHQQLHQQQQQTSQVLQQPPPSDLPQQQAPLVTSGAGTPLPAGPAGATKSAAVAVMGAPVAASADTTTGASGSAVAGIVRHGAGAGAGSGSGSGSGAGAGAGAVRIPQPSNLYGRMSSSTLLAKLPPTRVSPRRGGLAILRRSSNGTFQDSTAQQLQLSPYTNAATGGPSGTGPLLLLDTADMDHETHLSSSAPASPVRPRSPASAVITFAGGSVSPSGIRAATVSSTNVVEGVAVTPDGSSHSNPGAFIPVQQATSQPAAEANQFAGYNPSIEQILLGSGAASSSLRSMANSSPPRPDLQLQQAFRNLSSGNKTPRLATREDVWELQAALLRQVSVLASPSPELPAIHSPRTAAGVPLPAAIIAGAGLRNSVGNIRHFRQMQQQNALLPHAGAAAAASSNRGTLAVEGSHRVSTSPDISRRTTADGGPYPEISLPTIINVTSTSAASHSRWATQQPAQPSPVAVVLVGPPVGVAPFTSDDVAFLPLLTRDDEGYVLESLIAGQECALSTALTGIVAQVGVSCVERAHLLHRLWATMRQILAAVLRDRETARSQAAAASREASDCASRAAGEVGSLQQQLASLTQGLEDLTAKDREQTAELVAANKELEHLRQVVTACNVESLSARLVDAAEGRRRAVAEAARLQGQVKALEAEIHELRQEQAMAAARLTACESHTCALTAELRCRTPRPSRSLGILDELLTEHGSHKEAVLAALSTGADPTDLHRLMLGVTHDGEDLSRWVEVFGCVQSVIPPWGDAATHRAAMAPPQDTAVTEAANDNAASVAAMASAAVAGSGGGIAVTASNLGVTSPGTVGIVSPPSCLHGSIPSGAGGTGAAPQTQLAGALGQGVPGGTPPPVRRGVSFSQQVAATAAALAAAVTTGSRVTSGCNSPDWEHLSTPALEPPLAAGSGTGESAAAQAVGGSTLLEASSAGLLARGVNAARHRRRSSAVSESFGGGAGVGGGESAARRQVMSPPQQQAYMKRQHAWLVSAMEVGDVASLRRLFPDEAVDAIGSGLQLGVSSGGMAAWLLGTLSPGGFNFMPYKDLVGVLSRQFQHTPLDLGGPITSGLELAALSTVQRMKVLEDKVQQLHREAVRLKRCLADRKRAERAQVQAEANANERKAHDRPVHPVHTFLTAKWGDFFDGLGCGAKVPKVLHAEGRVFNSRLEKVDAEKLVNSIWAAKADWQSRNPGSQMSLADFMMSYMQRKYVNPRALTETAYTLIYTLGQHMYDPDCHIFIRVLLGEVDEGIRAEGEELQADLVEVFSCLDRVAHGRCTGYVPKRDMHTALKGFFPSKSRARFREVLEALDADCPDSEAVRYRRIFDTDQDLNQGVFAETIRTQHLEERLERLQAIEDALMGAVDKARVHHVPPSLLASVLKLAIPDCAEESVRQYLRLVYGSDAGIRAAEAAAPAANAKFRRAFESSNSSGKGGPNSKGGGSAQGSRSSAAGNPRQGAAPMTTTAASAAGSGTMGAEGAADTGSSPTGSGVGSGSESNGKHEEQVVVIPTAEAVVQLIRLSGWVPQLPADITPLVERLGENNRSLAGSCWSASSPGSWGSSEPATMASTSPRGAGGYSSGGAGANGPKLASWTGNSRAALKRLRYVQAMGALKEAWLREEQARVQHIVQLAHGGGAGAHCLPLMRGPAAPAAEHHNEAGLRGSNSGVAGVPSPEQPIDTDDTGAAAHATLAAAAATVGVKISSLASLPTADEFVDSKEPDVAALEAARKVLSAQFFAATAAMSKNAGSINTTGSPNANNSNGTGTTAGNDGAAKADLSLPNRRLARASGSASNNAAGAISATGGNISSRSPSPACGAATSATAAIAALEEVGERLQRLWALGGTMAALAEERNETFTLGRVPDVHRNVDTGDEPLDGSTGTRNVKHGAAAAGGARMAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3W4</accession>
    <name>A0ABQ5S3W4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="178"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="305" mass="29872" checksum="624A5F5CCBCCF63E" modified="2025-10-08" version="1" fragment="single">PTDSQSLCAAYGMTPAPLYDTATQSAILQRLATANAYWIGYVVNTTNGTTAAPPASWIDGTPLSYSLLPPNIGAVAGEAAIRGSNGDGGGGSVLCGALLPTALPPPAAVVPNTTSATNTSSTASGGIKGGSWMLSRCDAAAASPFCMGSALGPMLTPPGIIPLATTGNASDASGQVLYVFSGSGASAVTANFSTAASQCRQLGGTLAVFDGDGALEGLASWHSAVLGPVWLGLRLPSATWTDGTNLSYSNWAGGVTPAGINGACAMLALTAMGNGSRSSTSASSGVGGALQRGDWGAASCHSLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3X7</accession>
    <name>A0ABQ5S3X7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUS-like FMN-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007695</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF5">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="3">
        <location>
            <begin position="6"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="111" mass="12070" checksum="5D70557DA818BF71" modified="2025-10-08" version="1" fragment="single">MDDLPRVEALVRVLAQNLHIPVTPRIRIFPDLAKTVAYARMVEAAGAFLVAVHGWLHGQKDNTATRADWNVIRAFKAALSVPVLANGSIRHLADVEACLLYTGTDGVLSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4D7</accession>
    <name>A0ABQ5S4D7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="185" mass="19930" checksum="2526B0CC6E7942DD" modified="2025-10-08" version="1" fragment="single">MASSSDEPRGPLDTFAEDLGRAVLDAAASGNLSAPALLAAVTETGFRPDHLATLITQTTEIRTQLGKYLLSLLNLLKSTVAVNTNMDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTEKTAGHSIADATRAYVRDAKKYLQAVHGGDNDGIRCLFVGNTLEGSAKDWYREWTTAQGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4E9</accession>
    <name>A0ABQ5S4E9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007992</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046537322" description="Guanylate cyclase domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="2317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="819"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="4">
        <location>
            <begin position="867"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="728"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1209"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1613"/>
            <end position="1705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1721"/>
            <end position="1752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1799"/>
            <end position="1818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1899"/>
            <end position="1977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="737"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1224"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1620"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1721"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1905"/>
            <end position="1928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1943"/>
            <end position="1953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1968"/>
            <end position="1977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2317" mass="238103" checksum="CD3F30CC610B1EDC" modified="2025-10-08" version="1">MTYWYMKLRVVFLSVICLWALAGISESEDTGLLQNDYCVSAVLDSLIGLSSSPALDGVMCMNASSSAAAVARAFRDPRCLMDVTSNLKSSVMAAPLPSTAVSEPPLSALNNSSSSITLAWMQCPSWANGLIAEWLDAAYADVLKSLGRYEFEIIIQPPPPSNVSDAEAVHRVNLEVAGGAGGGSGINLAFSADASTEELEFLAATTEDFEHLPENSCSYDPHPLGPLHPRTFLLMWYRADTLSALGFNSPPDHWEELLDLLATHKKARTEEGASLPQYGLCITDNPHCGRAGDVLAAVAASVVQSRGTSQGYVFDLNVPPPAAEPLVNGSGWRYAAALVEQLLSYNAPDNDTKSFPKTPSPRRTKSLVCHAVSPYFTAGSCMVTLEWDAALPHMAAAAPLQRPDALGVAPLPGSRWVASTPASTDDLVLCNAQTCGVSVIHDFLYGMYGVYDESEAISSVINELVPAPWPPGHNISRDTPRALALVEAASIGRLGVPELAGAPAIAAASTATNSKFLGISITSVNRAPFSSFFRENTEVNYGGIVDAGGDITDLEVAFDSPRMNILERRLIRQAATDRLRGGMGWERRRTPSNASVCEQYVRTPWWRMVLQLQRAQNVSSRSSFNESMSANSTTTTTTMAAAAAALRDAAVTAATDMLRSNNIPEKLILPYLRALWHALHSPNSAPVVVAPAEPNWFRWALGHAASELAAAAAAAGYDADTVHSNGSGGFGDSNSNRMEQSSRTSSNISDGDDSVTAAVTDGTISTAVGLIDKYFRLVTSVLDSSVVRLRYETSISAPDWLDVGLEKDEGRSTALRGGALVGLVVGCQVALALLLSAVALLFWCRRGRRHRDLLGRVRAPQAGPDTTLLITDVQNSTVLWEALSVATMDAALQLHHACIRQLLEEYDGYESATEGDSFIVAFASPASATAFAASCQLALLEADWPQELLQHPDGAMVVVEPRVAGQWSTTGASHNGAAAAAIAAAGAISDISLNGRTSLHAAAASGMTGGAALRLFQCELGSTGGKLLRGISSAIARSVSVTSIGDVIATAPTSSLHAPSGRRAAMFGGASTASTAVASTAPRLPVNAPLTLSSDLPPSLPTAATQIAAVALPAAAEDAESYSRKPTIGNRIGSASPRMSLEGGSPFGAIGIAAAAAAAAAGAADPLPLPSSPAGRAVSGSLVRSEVALTLSQAHQGQDRRQRYSLHKHLRPIPPTSPSHHHQQQQSQQQQSQQPGPAMIRDSYSSCPEGTQRAIGSAPLECIINSGAGGYESGGGGRGSGVGMADNSGGLATTSIFSGLFSTVAATSKATGPATRSMSYRDALAAALSVSWLPVLPSRGGAIEPSSRSRPYVRLCDGRIVAFRGLRVRMGLHSGLSTHHHVFFNKVAASYQYSGEFAEVTRLVSDSAPGGLVVMSGPAFARLRHCVERGGWAGGGGGGGGGRAKEGGSRCSIAMKEIKVIYAGHHLLKIPADGAASAINSDSTVQAAVTAAAAAAITELAAASGSGFRSAGLSQQSEGESSSPEVNAVLPLSKAVPWLIVASGAGGGGGGGGGSISVGGGGGGGGGAPDSSVLLDTRIVDVENVTELNFESGTFFEGGGNWAVGASDSRQADPLAAGAASGGNVGGGDGAGGGEADLQRRRRQRRHGTVPEAPGPAAGKGMGDVAPDVATTAKPDPQLQPVRPQVEDIEYGEERLGDVGGGRRGGQRDLLNVFGVAPGCSGGGSSGGGNGSSGSNSGSGGTSSGNEGPIHGLWPASLEIVHQPPPPPLIPHQQHSRTYTQSTSSVRRCSFDAVAAATAASGQPVSPQPLTPRPSMQASRTQALTLWPYHSAQSSIPPVGHGGGGAATAAAATAVTPAASRCNQYDAQGLLDSLPNSLLQNDNNRHLHVRQVRQNDPDLSYFITLPPPPPPPLLPPPPPPPLLQPPTPAGRLLQSLAKRQHSTTRTADQQPCQAPQHSLLPPPPLPTLTPLTQPQSPEHLLKVPQPLYVAVPSGLLCRLALVSPLRSRWVSQLSSLDAPTGLITVAFLKVVGAATLLAELPGGVAVEALGQCQRLVVGRLGAAGGYLVEGCGDGLVLAAFGSPTAAVEWALDCLEGLRRLDWDPRLLAHELCEEVISFAPQGGLMIAGTAPGTATGQQAAAVAAPAVAVPAVAAGADKGPGSAIAGVAGRFRSGCGRGAGGGSTGVATPQSLNQQISKEHQSTGGPPVLSRGLRIKVGIDVGSTTCDLNPGSGRLTYRGRVMNRASRIAGIAAAGQVLVSGAVWEEVAAAVAASASASVTDGGGSPPPPPIAALSLGNVSLKGVKEPLEVLQCSLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S561</accession>
    <name>A0ABQ5S561_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007947</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046732448" evidence="2">
        <location>
            <begin position="23"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="508"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="543" mass="53615" checksum="EDF5A62861D7DB1C" modified="2025-10-08" version="1">MSFLSMRYLFLVVVLAAPAVLAQAPPDGGIIIMAVSQPPKPPKPPRPPPRPPTPPPKPPSPPPRPPSPPPKPPSPKPPAPPPPSPKPPPPPRPSPKPPSPAPSPRPPPPSTSPKQPPPAPPPHQVVSVATPPASQTPSPSPPPPSPSLSPPPPPPIPLPPSPSPSSPPSPPPPSPPPPPSPPPPSPPPPPSPPPPSPPPPPSPPPPSPPPPPSPPPPIPPPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPRPPPPSPPPPPPPSTPPPPPPPPPPPPPIPPPPNPPPPSPHPPSPSSPPPPNPPLPPSPPPPRPSSPPPRPPPPSPYPLPPPSPSPPPPSPPPPSPPPPSPPPPSPPPPSPPLPSPPPPSPPPPSPPLPSPPPPSPPPPSPPPPSPRPPSPPPPSPPPPSPPLPSPPLPSPPLPSPPLPSPRPSGNGNNGNNGNGNNGNGNNGNNGNNGNNGNNGNNGNNGNNGNNGNTGRRRMLRMTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5S4</accession>
    <name>A0ABQ5S5S4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01838">
        <property type="entry name" value="Isoamyl_acetate_hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045136">
        <property type="entry name" value="Iah1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209:SF19">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="2">
        <location>
            <begin position="102"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="358" mass="39824" checksum="984204A7441E5D5C" modified="2025-10-08" version="1">MINMNFSFLVTPEINPRHWRQGNNCIINVLICFFACFGILTPIAVPVILSDSQSGSWKINKIGHFLVGGKKLCTMASATEAKGAVAIAGSSFVAALRPRIILFGDSLTERSFDAPEGWGASLASFYVRRADITNRGMSGYNTRWALQTLPYVFGRERTPGVPLVQATAERVLFATVFFGANDAARIDGTSHSARQHVPLDEYRGNLKEMVRYIKATGVERVAIITPPPVSDTGRKIAQIQRMGEQARNWPLDRNFATSAEYSKAAVDVAKELGVPCLDLFTLLQDENWEQKYLCDGLHLTPLGQEKVFHLMRKLVQDEWHDILPHLLPTQFPAWDAINFDDIPSSFVATEAAFQQQPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6M5</accession>
    <name>A0ABQ5S6M5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008782</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="2218"/>
            <end position="2533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="11"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="60"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="190"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="456"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1211"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1402"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1490"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1763"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2019"/>
            <end position="2084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2100"/>
            <end position="2129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2607"/>
            <end position="2647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="277"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="476"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1506"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1546"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1805"/>
            <end position="1814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1839"/>
            <end position="1852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2047"/>
            <end position="2070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="2071"/>
            <end position="2080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2112"/>
            <end position="2124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2636"/>
            <end position="2647"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="2245"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2647" mass="269915" checksum="AEA4AD563D770C83" modified="2025-10-08" version="1">MQFVLRCFGAQHDSGSGQGAHNQSRASTASSQKHYATTTPAAVPVTARPNQSAQTFEVSAKVPQPEVVEPDQNGNAGSGGVAQVKADDEGDRQAVDCSFPRRLSLGSPLLLFNTPFVATHHSLTLKLQGAARPGSSAPAVDNWVALGSVADGRATADSERTTACSNPSQARLQSNRGDAPVLSAAGAIHGARPTAVQQSQPGSHIQPQQPDPQPVRQSQPSPNPQATARSSSDLQSHARTLDPRMPSVLEGSADNLRLSPSRWSISSSNQATPLGPGASRRQGSGAAAGCNPAGGHFTALPAPRPFPAEELAALVSTLSRVRGHWLEALHTVVALAADLLGADSLSVYLLSDSGATHAALACHGDAGGAIRVGMPLPAVAAPGQKQTALQRLYQTGEAQRGSVRNAVAAAPNGPPVPTANSGPMALHTVPGVGSLNHHSCRVQGFARGSALMQRVQDNPQEHQHQHQHHHQHQHNQTHLQRQNNNYNNNQQQPPVAALPPDWQWMRVLGGMTDFVALPLLRGDLVIGALQVGDNCTPPEDITGAVPTSRRCRGTAELSVVATFVGTLLRDHEVLCLMGALEAIASAQDVDGLVRSIARHVALFSRMRKHITPGVRVALIRGDAAALALFLDEGATAAAAAAAAANLSAGVAGSSTTPVGVLSTAVSRHSTVYNLLHATSTVGGGGVLSSGACTPDPRHILSPGASVSGMAAGLGLSPAGRASQIAAGDGGGGSGASRCVVPLYSPSVETRDPSSILDTEQLLPAAASVVCVPAPVGPRVHATASASVLMQPTAAATGSEDPAGILHVHGNMTTSGFENAVDGGDGISGIAMSNGVDLWPWFASTADRAKGTDFVGAGDGGHGPTAAGAAALTDADGGPGDTPESIMARCTALVGLPSVVSDEDMLGTCRLEHANMVDELPTSRIGDLRATVTDASGHRIIALRGASENMAASNTSLDGDPLGATNTTEPSLGHGPPALIVAAAAAAASGGLLLAEGAARAEATAVVQESVLAAEAAVAESVSVLMSLPSALLPVTHSAGPAIAQTRLGAISGGIYGRALAMAGEDSTTPGRGFGQVQGHAMLTRGTVLAEALAAGTGMYISDVAIYMQDGRNKSRDVFLAPSGRGGPTQPTNSIAVTTISHAGLPLVGIYLTYGTPLPASLLNALTCKVHAFGAMLGPLLHERLTSGDSELADEWRYLLGEVLDPQAIQVAADEQRSRRSSAAPYNQQQQQQQQPMAQGPKATAPHRVAKAPGVSSVAADSARRRSGHGDGPYPVSASGSRGPLAWGFWGFGSTAGGSPSVATSVMVAPLRRSQTTDSEFLLTGPATSRAFPSDGGNVASCFCMKDRGVGSSRASVATEAGAKAVAACTLEPSPSATANAAAGSRAGDRNFRNTCSGILTLSGGQHPASASGPSDEAYDRASVNRMPLAPPPSPPGRASAAEAMTSGVPQAPAIMSWRGAAATPALHGGCAYKRKAPVATASGPLWPVSQHLQQHHQQSQERQATPLRSPLRSPSRQQHQQQQQPLAARPLLNCSWRGPTTKGNLPGSSSGAASPPAAASRSSGLAPPVTLSAGCSTSKLEKAASEGIASGAGSHGLYLRNHSGAGRNLDSGPPLCNEYRRVNCGSSISSGAVIASRSTSGALGPAITTQAVAAAATSPLLQGGPWALDGLQTTVNNPVNPTAAAATGAGPEELPSVSDATLLLHGAADADAPGNDDACGSVGLVAAAAVAPPPGRLSDASGSAIRSRWAAVSSVHIQVPVEPNLNVPQSSPSVPVPPSASSEQERLMMQVCEERKSPSVPQPGKPKEPHEGSHLQRQPALPVNSFAARYTPSPPWAPQQQQQQRQQQQQQQSPAVRGASVLNGGARAGGERLAGAAATVTAAPHPGCFTVSGPVVAAIPEISSSTGTMSMSQLELAVYPRLLPAYLATASSVGGGASANRIAGTAANPAANGASASTNSHNVDVELSMQAWHLSSDPARQSIVTPGSRNSPAGSAFGPASFNVSLPGGCQDVISTGRARQVQPRAYMPVPSPLSPGRQFHGGGSGSVVSTSAVAQQSTRRCSAGGMAPASPQPPPPPPRVGAGLSRLTTVATAAGVSGGSAAAAGGGTGPPQFSDTMPRSPNGNGFHGAVGSGAAAAVAVGGLPSVKAPSPMVHRLQCSVRRNTSFNIMADPGVQDTGKVAPLMANLQDKLRSLQAEQVLARLHNKDASGVDELSGLTILEPLGSGGFGTVFRGIFNGIEVAVKVLCERGAGGRSEAATMREAFELAVMTTISHPHIVQVLSAWTDVILTSKPNATGAPSTTTTAVVPRSPATEELGDATVVIAMEYCDGGSLADAVSAGRFRQRVPGGYGALQPCMPAIYATLLEIALALRHMHAMLLVHCDLKPANILLKSNMRDPRGFSAKLSDFGLVKMVVEDGTGGGGGVIGRDARTGTVTHMAPELIRATEASKMDASVDIYAFGVLMWELISTGPVYLGMRSEEIRRGVVDGVLRPEFPQWSDDKYRALAEACLSTEPKARPTSAELVGRLRTLLAESGALRDTIMRRPCRNSAFPAMNNLPRLGRAAAAAPPDASAIGDGFPGHCTAAAAGAMLGIGPADFCRPSRLQAAHQPQQHEYPIAAGAPHSRRPRSRSFSGNDSSTFPLHIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6T3</accession>
    <name>A0ABQ5S6T3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="573"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="680" mass="71931" checksum="06990610E2A3D97E" modified="2025-10-08" version="1">MSEDPAVRAPIRRIKIKFGNKLVSTHDVRVTGLVSSASQDRKDAKQGSSLQLAKLRQASLQGYVPPSASADLIVARLREGEGFAPGSEHPPWDFKAPPIPCHVDRPSKRSRPLPSCAEDICRLGQTGPASSAATAAGPICREGLTAQIEGMSTEVGAKTEITSEPPAIIIAVRSGESNSDQATTSQVYLPLSPDGTGDHTARAWPAAPASGPLQTWASSVGQSSLATTVEAEAHRHPQRKRSNSTSKPPTTSVDVAVAVAETSAFAGSDIPVKLVASSAVLHSAGTVAAVEAQAQPPPRTGSSTHACGSCSPSPGSTARGNVPSTSGRHPTSPVPVRGPKPSSGISVDTQTQHGDFPLLPLSFLEHPRLCGTALSFQSIGPFDLQLEREERVILADDQINDLMARAVRVKHEGDKLSLVPNSTQRLWTMEALSKYMTSSLLFMEAADAMAQRREHKYNPRGAAELYRGTAEMLYTSVSIAENLRGNSLAKEGIRLLADRLCAICSLRHTSLSSNNFKACADRVQAALRAQQQQQTSQGAGAAAASTRNVCPQPCTEDSSTSSLQVPHVGGTNSSRTAGQGSASGAGSSAPAQPLAGFNHEQVTELVGYAHSTCRFAECMKRSCKSFEAFLERPDVRQDQQAKLVCMHLAAICMDVGMTPGHRLLRHAKAALRTLVRDLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7M6</accession>
    <name>A0ABQ5S7M6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NYN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010298">
        <property type="entry name" value="YacP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34547">
        <property type="entry name" value="YACP-LIKE NYN DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34547:SF1">
        <property type="entry name" value="YACP-LIKE NYN DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05991">
        <property type="entry name" value="NYN_YacP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="37244" checksum="84A6491E4BEC204B" modified="2025-10-08" version="1" fragment="single">MKYEGLTLSRGHHRLLPKLSVFLRLPSSTLTFRPKWPSSRNTRLASAASAAGRGTPGDKDGASGKRLPQQVCAGAPSSPLIHHICCVQLDDSEDDVPQPGQPTILQQRPTAPLRRKSYRRTKEKAEESRKQRAEYRVQLAAATKQGRVSYIDPSSTPAPDALRVVLVDGYNVVYACPELRRLADFSLQDARYALNRMAVAYAATYDIRVYVVYDAMGSNRNVNTLERLSPGAVAVFSAESEADTYIEKAAGKLKRRGAQQVMIVSNDRYVQSNAIDQDYVIYPVQLDTWLDQAHRAVTSGVRTGRPGGTAAVPGPVLELFSPSIASVSTVGEDNDDGEVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7M9</accession>
    <name>A0ABQ5S7M9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046338962" evidence="1">
        <location>
            <begin position="30"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="106" mass="11590" checksum="0D9A9E7A2959AAC1" modified="2025-10-08" version="1">MTARRRALASRLAAAVLILIFGGAAIVRAVNTTLCVEVGMTLPDNPEVKEFLKCANPVHILPSCCRHLLTLTKYYDCLTVPAFVDRINRFLNGKTTVNGVQQDCVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7S6</accession>
    <name>A0ABQ5S7S6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar fusion protein MON1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026069">
        <property type="entry name" value="Fuzzy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13559">
        <property type="entry name" value="INTRACELLULAR TRAFFIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13559:SF1">
        <property type="entry name" value="PROTEIN FUZZY HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="661" mass="70101" checksum="DAE716EDA678700A" modified="2025-10-08" version="1">MPQLFLILEEGNTALLTRALGNIPTPRFASQGLLSTIASWCLESCGHELRVIASTNVRVVYRRFSNHLLFVLVTSNLLLHTDLLQSHLETLYALLLLLLGPTTLVLDTFAKINILKSKLRAPDVQRAVDSLVADDTLVPCILLTVPQLVGLSLRERANLQSGLVKIVSTLGCQHAALIGEGYYLSATPGWWTLLSPREQLLVQHVSTQHSKHHSDGCPPELQLHLSKRHYQAPLRLRILRLQGAVLLAALYESGGAPPPPLQDHEDMVRSHLNAVRYTLERPLWLSPPVPQFATISFDLALVVQHSPSSAVLLWGHGGGAAVQQGFNPLVSPSLRKQVGSSTSPIAARLANRPPSATREESQTAGGDGLQLFFRSTLDTLVSFVLQTWPQMPAELQVALQDRKADGGCGGGDPSGCNLVVQNQVKPRAMTTAPATTTIITTTTASSPPRGDHLHLQNNNINLLPQPQIHHYPNRSAPPAGAESLVMPQPPQPFVPLSAARGTSSVSRLGLGAIRRISSTALGSSMQGASRLQALFGRSSSAAGPVGVARGVVQGSMLAVPSGAAAAGTAGPLEEPAELADVPYEEVHAVIEGARLLALLDRSGRASAGGPSGGTGTGTGTGAAADVIDVFVAFPKCIGRMAALEAANTLRSTYLMVYHDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7Y1</accession>
    <name>A0ABQ5S7Y1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="218" mass="22675" checksum="0FDDB9743909880B" modified="2025-10-08" version="1">MHGFSSRFGAGSCRGWSPTGDGRAGGSEETLFPSESLPNSWPSTAPSISAAAQELQFQREGSSREGATGSRCLDQGSEMQRPMVAPPARLLPNLPLAPRDVGILSVGYAASIPVVPCEASKSVSKVPETQQGRAGEYGRGNQEGQYEMGGRDGGDSDGRQCANPATVGSAHMVESLVGGRVGDVPLSVWAVWMGWSLCEVVASSPSASQTGCKWRGNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7Z6</accession>
    <name>A0ABQ5S7Z6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="51405" checksum="D7298BC234AC83FC" modified="2025-10-08" version="1">MQQASAGSHKANLPKSTHTSFFLQSHAVRTLLPQRAPWRGRQPMHGIPGRSVSPLAALPESGIRKGTSLLEWTNKILPQGQLVNGVRQGWRLAWLAMMQELAPQTAKGQYSRPQYAFNGHIGDAQFPSESGRYHLYLGNACPWCHRVALAAVLRGHLVAPTATVLPQQQRRQLPQRPQPLQHVTLTRLEDNPTRARRGGWVFTAADPDPVFRAVDLWEVYDTCQPGFRGRCTAPLMVDRVRRVEVCNESAEIVRSLDEMHLLGCTDVQLRPKHLAAQIDELNAKIYSAINNGVYSSGFATTQEAYETVQAEFWSALDEMEARLKSSRFLLGERLTESDVWLFPTIVRLDAVYGPLFKCCRRRIFGAASPSGAVSGGGGGFGGAGDYPALAAWARDIWQIKVPGSRLQISDTLDLDAARSSYFRNLFPLNPGGIVAAGPTAADLRLQDLPAGRGSTALQDMCYPRDSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S881</accession>
    <name>A0ABQ5S881_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009866</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050154">
        <property type="entry name" value="UbiB_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566">
        <property type="entry name" value="CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566:SF119">
        <property type="entry name" value="OS04G0640500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="3">
        <location>
            <begin position="371"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="533"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="777"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="956"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1047"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="539"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="958"/>
            <end position="973"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1073" mass="112840" checksum="2DE21C04A4644953" modified="2025-10-08" version="1">MAQVQQLMGGHPSSASDSRFQSRRVAAFSTAQPHLRKLRTSVGSHNQGGRLADGASLVFPVTPKPTWPWRSGRRRLENFFIRARSSSAVSTTSPASASSASTPTPTMTPLYAASPSGLSGNPGLSNRALTSTNTNLATNSYDSDLQTDRVNGVMGDRMRVSGTSTSTSTASSTRWGSGTNISYSLGTGNTQDRSNSATSVTSAPGTAAGALRASSTGPNSPALRPTSNLRAMAATGETTIRATAAGSSFSGTVSSQKAATAPGADDSDIDIPEFMEPSTYDAAMNSRYWETRPVRVLSRLLRIALEFGGWAAAARARNLVVKDPAAQEEAAAESLKDVLVRLGPAFVKIGQALSSRPDVLPPSYIAALEALQDRIPPFPDSAAMAVLESELGVPPSAIFSSLSASPVAAASLGQVYRGSLRPELGGGEVAVKVQRPRVAQMIAQDVYILRILAGWLRRARRLNTDLPALVDEWASSLFRELNYRTEAANARRFKELFSHLPQVYVPYIYDEYTTAKILTMEWIEGERLRTAGSSVSERAPPSNNASATRSSRASIGPLSDSQRLEDLALVEVGVRCSLEQMLEEGFYHADPHPGNLLRTADGRLAYLDFGMMGQIDERTRNALMTATLHLVNREYGRLAEDFITLGLLPSGTDRDTVLPALTGVFTEALKGGVSNLSFGQLSGNLGVTMYKYSFRIPPYYTLLVRSLSVLEGIALAADPNYKVLGSAYPWIARRLLTDPSTDLRKALHRLLYTEEGQFRFDRLESLLRQAAKSANRLKRPSQLQGGPPTALTSVDGGATLPAVTTATTGGTSSTPGAVKGSGGGGGALLLLLSRDGEFVRGILLDEVAKGIDGAWRIAADATVATVRQGVAGLPSPALPLLALLPGAPLLAMLASGRIDGGRTSEGSSAMSVQQSLSSALAQLPTLATADDRAQVAGLLQLGNALADVSGLKLPAPPASSTTTTMNPRTGTGTAAVESAGPRSPSPDPADLYGAVTQASEAVLWLAREAIELPDEAKGELLQLPLQLGARVASRVTARALRTLVVGPRQADGGSGERGEGSTAETELVPPAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S896</accession>
    <name>A0ABQ5S896_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TsaA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_009717</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818:SF0">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="5">
        <location>
            <begin position="208"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="95"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="425"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="35"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="425"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="511" mass="55145" checksum="C00219899735792B" modified="2025-10-08" version="1">MAWDRQSVLERCAISAATAWGFYLLARFVNGRWLMTALQQQLQETQASLEHERVKRTADRVGRIRAEQELRAVKVQLQRLQLQQQLQPEATAIASSANGDTQSDLGETALPLTKTTDGASGPAGPPQPLGREADETQQNVQHQGARGKEQRGRGDMEAAIEGASEPKSPVASAATGGLAGHHLDAKRNAAVAAAALAAAGSAMPAFPFRPIGHLQSVFTERNGTPRQPQLVPAARARLVLCNNVPPACLEGLHQYTHCWVLYVFHANTDLAKYLSTDRSGLKAKIHVPRLNGARMGVLATRSPHRPSPIGLSTARIIGLEGNSLLLGGADIVDGSPVLDIKPYVPFCDCLPAAKAPSWVQAEAEEEPLAVREVVMDQTAQDALMRAWQARKAHSMYSSAEQYLELVRQVLSRDIRSVHQRLNVRPTSAAQSVATAGPTTRPKAPTPPLLVPRQLQQPQPDVHNGVPGSSVTEGVQPGEYHVVLENVYISYDIGPSGVVQVYNAWVDPITAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8M3</accession>
    <name>A0ABQ5S8M3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rubisco LSMT substrate-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_010049</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000030">
        <property type="protein sequence ID" value="GLI66288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF136">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rubisco LSMT substrate-binding" evidence="4">
        <location>
            <begin position="309"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="485" mass="53008" checksum="150858A1BC27CB0A" modified="2025-10-08" version="1">MQQAFKAHVPNRASKLQRAGCKRPTLFTCRAAPAVASSVSSNGVVSWATSKGAKLERASLSTDLLTDRPLLIASADAQQGDLLLSVPDSVWLSVEAVKKTTVGKLAAAEGLEPWVQIALQLLADRFGNSSSKSELSAYAASIPENLDIPLLWSDDELRVLQGTQVLQTLGGYLTFFQATFQQLQSSLFAANPAAFPPSTFTLPNFLWAVAAVRSRSHAPLDGAKIALAPLTELVSHRRSANSKLAVRPTGLFGRGQVLVLEATRPIRKGEPLSMDYGPGKLDGPVLLDYGVVDATSPKPGYSLTLTMPSSDRFIDDKLDILESNGLRQSMTYNLTPDEQPTTEMLAFLRLMQLKGMDAFLLESIFRNDVWGFMQEPISEANEEALCTTLLEGARAVLTGYTTSIDEDLAALRGGSYPKGSRREAALLVRLGEKEALDAVARFFEARRETQLKRLVYYQERRLRRLGLVDDEGRTTYDSFFKDGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8Y1</accession>
    <name>A0ABQ5S8Y1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_009245</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="49"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="166" mass="18222" checksum="01C5F8B727915C21" modified="2025-10-08" version="1">MALTLFNDPFMSEMDRAMNRMLSSFGMPAARSTAASNWPVDIFRPVMGTTAGTASMPMDIIETPKAYELHADTPGMTPEDVKVELHEGVLTVSGNRKVAREDKDAQGKVWRSERSAYSFARSFTLPENVNADNICATIDKGVLKVCVPKKETEPKPEPKRITVTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA54</accession>
    <name>A0ABQ5SA54_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000042">
        <property type="protein sequence ID" value="GLI66812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052160">
        <property type="entry name" value="Gypsy_RT_Integrase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47266">
        <property type="entry name" value="ENDONUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="1">
        <location>
            <begin position="120"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="184" mass="21556" checksum="1EAEB71544D38264" modified="2025-10-08" version="1" fragment="single">ERLKVAYNADAWFADPANVENLRKQNGLWLRTEGAATQIVVPNDDALRYVILARFHEDPLAGHPGSKRLIDLVQRSFWWPRLAKDAANFVQTCSLCQRNKALSVIEPVPETWIAGKQKFYDVERVLDYRARRVGRHRRKRTVHEYLVKWTGYTDEHNSWEPARNFSQDMKPLLEEARKRATQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAB7</accession>
    <name>A0ABQ5SAB7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010769</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF156">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q-LIKE 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="70"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="178" mass="18956" checksum="BCF7A2541415B23E" modified="2025-10-08" version="1" fragment="single">GNFVLQSRGGNGVLAAYPQEPFQLDQLVPDMPSTDQIATDPWVVSDDQLAVVLRTVWGHPGFRGRQLELVRAVLEGRSMLAVLPTGAGKSLTYQLPAILLPGVTLVVSPLLALMDDQLANLPPCLRGRGACLSGGLTKEQVDAALAGATRGSVKLLYVAPEKLLTPWLLAALRRLNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAF2</accession>
    <name>A0ABQ5SAF2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000047">
        <property type="protein sequence ID" value="GLI66902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="269" mass="28213" checksum="F314DE616D85E809" modified="2025-10-08" version="1">MVLCCGDEGPGVVRDVRVWPGAEGLELFRLQLHALLGGPAHEDDVSFVVRIGREDALILLPGLSSYVVASYCAALGAVLGRSSTALGVKSVQTAEPQRKMEGGYEGSPREPQLPQQCKADALPTGPAADSFSSEDDWINLLCSVADLLEGEVTAMTGNLSQADLRKGPTMSAAEARTQIVASTMVTVWVYVRRRVPSVSELMKPRQVSRMATAGARLLSDSSAGWSVSQPTAAAAAGKAAAAMSPQHPLQSAMQVHPTQSLSCGVAIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAH0</accession>
    <name>A0ABQ5SAH0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plastid lipid-associated protein/fibrillin conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010966</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000051">
        <property type="protein sequence ID" value="GLI66927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039633">
        <property type="entry name" value="PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006843">
        <property type="entry name" value="PAP/fibrillin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31906">
        <property type="entry name" value="PLASTID-LIPID-ASSOCIATED PROTEIN 4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04755">
        <property type="entry name" value="PAP_fibrillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0934">Plastid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastid lipid-associated protein/fibrillin conserved" evidence="3">
        <location>
            <begin position="53"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="222" mass="24764" checksum="7FCD4007B0312B78" modified="2025-10-08" version="1">MANGIFHNCTQKMALVRPHHRIATATRMRVSPKSGFFSLLTQRTNKVEKLLAIKEELLERLEGLNRGASASADDKAEVERLARSLEQLNPTPQPLKAPELLNGKWRLLYTTSAFILATSRPPALRPQGPIFQTIDAEKLKARNNETWPFFNQVSADLIPLSPKKVAVQFRTFKLLGVIPIAAPPSARGELAVTYLDEDLRISRGDQGNLFILDMVDRSARLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAV6</accession>
    <name>A0ABQ5SAV6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Large ribosomal subunit protein uL18 C-terminal eukaryotes domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_010548</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the large ribosomal subunit (LSU).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025607">
        <property type="entry name" value="Ribosomal_uL18_C_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14204">
        <property type="entry name" value="Ribosomal_L18_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00058">
        <property type="entry name" value="RIBOSOMALL5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL18 C-terminal eukaryotes" evidence="8">
        <location>
            <begin position="236"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="248"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="263"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="293" mass="34097" checksum="F18BC90C3F9EF4A3" modified="2025-10-08" version="1">MGYVKVVKTSAYFSRFQVKYRRRREGKTDYRARLRLVKQDKNKYNTPKYRLVVRFSNRDVTCQIVRSTIKGDIVEAAAYGHELPDYGLKVGLTNYSSCYCVGLLVARRILTKFGLDKHYPGQEEPDGEDFNVDPVEDGPRPFYCLLDTGLKRTSTGSKVFACLKGALDGGLDIPHNEKRFVGYDRSGKKFDAEVMKKYIYGGHVAEYMEEMEEEEPEKYTKHFSSFLEEDISGGDLEDLYMEVHKNIRENPLKEKKPRTKPATQERWHEQKLTLEQRRENLKQKLATLMDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBB9</accession>
    <name>A0ABQ5SBB9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011369</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058719">
        <property type="entry name" value="WHD_LYAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF9">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25879">
        <property type="entry name" value="WHD_LYAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="4">
        <location>
            <begin position="19"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="93"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="371"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="471"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="305"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="480"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="641" mass="68222" checksum="627D051EE4EEF4E7" modified="2025-10-08" version="1">MTAMAKPLMSAPFFQGVDKSSAGYRLLSSMGWKEGEGLGAQKQGIKEHVKVKKKHDAMGVGAVENSQNLRDWTTGMVSFDRILSNLKEVTANQRADNCDDSSGSSSSDEERAKEQAKMTKQNKAKGAKRKRASPSSDSKTTTTIISSDAESNDSDHRKSGKAAPATAVAMTAAGHGVKRLKLASHVGRYSKRERAKFVKAYSASDLDAILGGVAGKDGAAAAQSDDDVDAGGVGAAAITQVPDAMMGFMPVIAEVRAVQRSQGGASSSSSSGGEETDAGGSAQRRRAAGVLQVDITSVAVGKSRPQKEREQLKQQEYEPGKTPWWASMFVRAGRMGTIRQELRAGKEHLSKAKINVTGFQEQDQENLYEQAQHGAAHGRQGLGRSDMPKKVAGARWCGTKTKLDDDDNDEPQRDTNRKSEISGSDTSSESEDDHVVVVWSKKEQAAAAAMAGTAAGTPSLGKIAMDAAEHACPDKEAGSKQKRAVKNGRKVKDEQEGDMAKQQQDREGKKSKRKKKRTSDKMGGTEADDEVEASRPVTGAACAGSAAIEAGGRAGTGWYCGNGAVKRKKWRKLVRQLLADAPERRLKLKKLKKQLEVAHGLGSGIGAKGADEGSLGMAAVLEQLRSSKKFVVCDKFVSLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBQ7</accession>
    <name>A0ABQ5SBQ7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011530</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="128"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="450"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="486"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="531"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="589"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="657" mass="73023" checksum="5312DD74370E1B75" modified="2025-10-08" version="1">MSAMKGGRQRHGSTRGSAGAGRSVTLVTNKYGVRPLQNGTSAAQLQPSQAEFSGRNAVPTAACARGSRPSSPFWGTVLQRGQSVISRSTDYVASASDEGVLRLPGRTELEPQMVTSVFGYARNLNEKYHLGRVIGAGSFGVVRECVEKATGRVNAVKTIPKVPKRGLPTPRYLLKLRTEVEIMQQLGYSLDAVNLRDVFEDNDAIHLVMELCEGGALLERIEAHKYSEKFIARLTRSILRFISQCHAKGIIYRDVKPDNFLFLRHDDESPLKATDFGLSIRHWPDEPKLTSRSGTPAYMAPELVLQSYDEKCDIWSVGMLTYQLLTGRFPFWEDVRTQTLSDVWKAILTQDVNWNAPELKQLSPSAVDLLKRLLQRDPALRPSAFVALEHPWLSEDGRARDLPLQGSVVQRLQRFSTFGHLKQLVLKMIVDEIQDEGKQGKAPASRKARAALGNLQDLFNELDTDSSGAITLEELSAGLRRQGYVLADNEIENLMRRVDFDHNGTVDLSEFITTLMDWDQVQTEQGWQVYLDHAFRKMDHDGDGYISLDELLQQLPPLRPAPGVGSLAQVVSGAAAAFGFTRAYDGDAERLAEAKLMLREADTNGDGKISREEFFDLLKESHAPDSLSFYDDRLAITSDGRTSAVVSQQSRQATAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC17</accession>
    <name>A0ABQ5SC17_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045591863" description="Sulfotransferase domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="695"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="432"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="79337" checksum="C4786ACDADA62831" modified="2025-10-08" version="1">MRLAVTSLLWFLAQALTTLVDAKQVDVRVLQQDVQQDLDHDISSSMLDEYFQSVLLLLAQANSQSRDRLQSDSAETFGIPGGIKALDELADSWIHARTTVGLYHVYELDDPLAAPLVTALSAQVPTRQQEASKYLRILMTAIRNLLREDGNVHDGSRTARESGGVGMVPEHSYPLLRRATVARLTQLWLLHLRHHDTPMPQSEYGSGSILFGWVATARRDVGHVLLMLFRLHRAGMRARIALEFLHISKAAGTSMCHLAEVSGCHAQDFSMGRTCLMRDFDDQPRWINGTHHWKLIAPVAEPWGAHPYAFLLYGVPRLPNRKRTCSARLGMLEKHRLNFFANEYTIYNNLTAYIQPEEDDDDDDDDEGDGLGAVGVVGAVKVNAPPSPPSPPVPRPPPAPPQSVIFEETAARYATYVSQLVAADSGTGAQHRSAGGSSSRSRSLKSAGSSSGESRSRTLRSGSSVKGRSRSRRVSAVIGGAALCPSFANVVVLRDPAERFMSHLKFIAQTYGTKYKWPGIAQAFAPGRNSPDWWRAFIPPLFDNYMLRTLAGEAAFAMSHEALEASAEAYMRLAKAMLAQYDVILVLEVNETLHRRAVHFGLAWRHLLTEFQLRNASALLKKLNEMKIFGAQLAALLPDESLLAELKPAAVRYDNQLYSYGTLLAGLDDVVWRSAELTLGEPMSGFQDGSVSGCGYIPRRERPPAPPGKTRDTAPQTQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC54</accession>
    <name>A0ABQ5SC54_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="182" mass="20198" checksum="BE654EA681444268" modified="2025-10-08" version="1" fragment="single">RQVTLRRRQLQRGEQRLKMARRQDAAAEEQVADLKLREEALGAAAASAAERESAAGRLAAMAEKKRAKAIAKLSERRALQAKLQAAASLHSEEESTAASAEQRLTARIAELTGELDKWRNELALAEELVEKLQQQQQQREGESSEDRHEEQHADDKDEKQGHRDYAGVTDGTAPAPSLKRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCM6</accession>
    <name>A0ABQ5SCM6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011918</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit p55 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040896">
        <property type="entry name" value="RPN5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18098">
        <property type="entry name" value="RPN5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="269"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="489" mass="54755" checksum="F7831832D7A4A43D" modified="2025-10-08" version="1">MSVGEFTASNIAMDMDEKEKGKAGTVDMAKLFADIALQKEMAPKGQLASAIESLLNLEKSSRLAEDVTATKACCAAILDVCYEARDWRALEENVILLTKRRSQLKQAIQATVRQAISYIDSIEVKDTRVSLIKTLQAVTEGKIFVEIERARLTKRLAAIKEADGNVDEAADILQEVAVETFGAMAKTEKIAFILEQVRLCLDKKDFVRAQILSRKVSPRAFVERKGEGQGEIGIEGTAIEEADKGTPSLAELKLRYYELLIRYYSHLNNYLEMTRCYRAIYEVHAAAEDAEKWVPVLKHVCWSLVLTPAYSTPHGSSSDRTTLLTTTLQDKKLSELPEYKTLLTSFTNTEIIRWTVFESQYEGELAAFSGGLFSEPKRHEDLQLRIIEHNVLVAAKYYSRMRTSRLATILSLTPEQMEKHVSDLVVAKAIKAKIDRPAGIINLAQPQSPEEQLNSWAGNIARLLELVDKSCQLIEKEAMVHKVTLAARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCM9</accession>
    <name>A0ABQ5SCM9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="487"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="539"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="617" mass="58708" checksum="8CC1AF21C7303ADA" modified="2025-10-08" version="1" fragment="single">SGGDAGGVGLGRGAWRLTQSLTSLARNLMGNKTQVAPDRTQGSSLIGDTDDSETGVGLGGKKAFQMLPGRSTKAREDLEGVPEDDEEGLDPETGAGGSIVGRALSGTDGLGNDSLDSRKTSFIQQTYSPPPRPVTALHLNGGKAGGGAIANGGNEDVQMLSSRSVDGSATRNSNGNGATVAMPVAAAQQLPGGFSSKALAAALAAAGAVGSDDATPTKSPAFLQHPAAPPPGFSDDITVLSSCEARSASGTASNVNVNGMGEACAGSRTAATISPTAASLPRRLTTQAAHDSANSSEVMRRSGSQNSKSSGASTPGQVPPSMVSAPSAPASPAEMAAAAAAAAGASNATSAAAAAMEQSSGAAAMAQLPSGSPDHEGNDLLPLLPTEKLQLPVGEDVVASGGGGGGGGGGGGNLLNGGAGGGSTASCSGSPVPERILPHLSSAIIQSAEEVAAPGPEALETASPWLVPSSSGSQMALTGGGVRTQSAGSAASASGPGKAAKKPAAGAAATATATATAAAAATATATAAAAVTATATATATVATGPVNTTTGAAGNTAAAGSEGNVGGGGVRGSPLPMEAASDLSLQPVLPAGGSKTTAQEREEVRTLAASSDGAADQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD14</accession>
    <name>A0ABQ5SD14_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="671"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="961"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1264"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="685"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1276"/>
            <end position="1300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1308" mass="136187" checksum="D3A1C4C0860310AD" modified="2025-10-08" version="1">MRGKRAQAAPSQQPNKRQKTCPQEEGASSSDIDMLQMLPPEMLSLVIGNLTPRECQPLRLTCKALCEVVNTQVADTLTVTTDMAQYLVLEQVKHSRSGAHILSNGWVARLLTKFPKVKNLDVKCSQNVLTLFARHMVRDCLGSSPPGSVPLSRIFDLADHVKTNSRRHSAVLICELIKFKVAQSLTSKPPGYEAMRQDIKQYGRADQLLTEHRIELQARVIELVEKVLTLKGHVDARAHEHYKYARDTLDDSLLQIVSCLQPRHSKYAYQLLAKAADPAKAARGWRWLFYFLARQKQAEAAIEAYKVVDKQALAQLSPTCLSELFRSLIDNEITTQPESKLRAITSDSGSWTKFLYRGNFTLTSTQSREGALEKVAADLIGKDYLDTAVNVMNRFETEGTAMEIWTQLLGALGVVPGGEMPLVTRDLHRVVSDQVHKASHLAMQAQPARRVACHIPLVEVLVAEGHLEEAFNLVATYPEQGALDKHLLRALSNVFSLTDCPPAHLSNATCSLLLDVLQHRLQDVASRSELTTILVAAYAARKDLEMAVRLASGLDAGAAAAMEADAGAAGGLALEPVPAAAAQGAVGLGNGGIVGVDGGPMAGADGDGAGPVAVTGLAGQRLAEMWRGVAVAAIRQSDAGVLLTALPRVNTSWLLGPMHEDLGIESASAAAFPDRPGAQPRSRPADAGPGLGPAAGAAAGGNAAADLPASGAPTMAMTMCSGGAMSVPLGPTTVWQSVCSVLLRAGRRVDLELVLLELLAERRRVLGGHTDQRALLESTARAMISNGYSTEALTLARRLGGGYASPSEEACGASSTVAADVGTSAGAGLSPSAEIESSAGTSTGVRPCWSVLNVVLEGCMGAGQLQLVLQELFAAGAAQSKAVGASPLLNNNSAGVAGNVAAGLVLHAAAGRLRQSLLADVLASLTEAGRLSEAVHLLRVLRGGDGDGPLLPVPVSTTATAASQRAGSGMARTTRANRAVAKRNRGNSASASAADQGADGTAVELAAVVEAEAGTGPGQAGTIQGVDASNLAELGHCPQPQYSRLLLEGSGDEEAGQATGSGLAISSGAAARVTGNVGSGPCTRAASLEAALAALVKALLKRVSSSESCIASGLTSPSGSSAGFGGAIGGKSSGGIPSGGDMLADWLEVCKAWPLGPSRDAQLDALARRAMDRGRDLEVALQAGWLLGEALVQEQRFAYTHRDLAQKCMGHGMLEAAQACLLRVQPHNREFVDSTARDLVRILARAGDKPAAIQLATSWAPSEEQRAEMVRSIRTARSAASSGGAAGAAPGSRAGSSSRGSSKDCPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD84</accession>
    <name>A0ABQ5SD84_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045464">
        <property type="entry name" value="Hrt3/FBXO9_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874:SF9">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19270">
        <property type="entry name" value="FBO_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="40"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="343" mass="38512" checksum="CA7C2AFD4E2BC444" modified="2025-10-08" version="1">MTLHILPVECLFGLHRPWLSLYGNRLQVPSAALEKETELALIQQSIPNELMVIILGFLEPYALGKAALVCRQWRILSEQHPRLWEHACNEALSLSIPDRGERQKLMATKYRFSWKRMFIHHPHLRFDGLYAARNTYVRTGVVEFTNHKPVHLVSYFRYYRFLPDGTFLYRTSPYVVSKVAKSMAQLPFPVLCAGSGSAAGQCAAVRLPRNRGPGGPGGGKEWQAADKDGAAVLWGHYVVKGNKVHCTLVYPNSTSTELHCHLAMWSTHPGASNRLAIESIITRDRELGSTAELVPQQEPNTDEGAPSVKSHTRGLAPCVFVPWEQVHTSPLNKLSHEMDFMIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDJ2</accession>
    <name>A0ABQ5SDJ2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011706</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050776">
        <property type="entry name" value="Ank_Repeat/CDKN_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201:SF15">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="979"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="195"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="829"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1057"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="234"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="829"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1245" mass="128350" checksum="95EAB6E19F9386E9" modified="2025-10-08" version="1">MNSNGHLMWPVSRLATIQNEETGMKTDVTMIAAEHLQAAQYRADDIAQSMRSMSTPQRRTRVILEPFEVQREVIGSRVAIHTRRSSAPSAATMRLAVQCHELEGNLQDDVNPEAQALGLGAEEAVALQAQLFGRSSGTVGTTRLVIPRERRRSSWAPGTLLAMQSGSRPGGHEALSPEDLYRQFITIRDAVLRKGQTHGKRSEEVEGTQSAVRSKLPYRHTPQSRGMSMDGEHAPGGAGDGPAGGCPLGSQAQRPASAGCDRTVMLAVDEAATLTAATSSTASPPASPYKSAVAIAAYAACAGTESPGAFRGVAEASASCGLSGTARPVGVQSAGGKVVHQRPPIQSPDLIDLQAYYYSPAASSSGADQQMSPLKAAVATNASDNVSYNSLYGCNRSNDSTQLRNQSTQGGGAGRFDFSLVRATGTMEAVSSIPETDSLFTLQPSGEAQPSTLSLGNAASCQVQQQERSSQPLESQSSAWQVRMAVKADMPVAATQTRQNGTAPGIAREPVATANSTVRLSSTHCLEGSAGRSGVVMSDSVAGAAENKGADMVVRSGRDGKEAFTPDYLRAVQSAAQRTLLPLPRVQSSSSIRSGGACGQDSSILSSGWAAVAAAYNSAAMQRKPAAASAISDGEDRSTYEGYVTLPLPGLPPPLPVPIGMGVSALPPPLAMHASPSVAAATAAVVTRCLYKGFPFAVIPAKHYAAMLTAAEQHDGPLVFHIVSKHCVYYPGIGRHAASSGGGPSLGEPACNGNADASSGCPRPTASSTQSKAAASLAASTAASCVRQLLAEGDALHVMSPGEYQRIQLAAQYSLRQLQDILGVQPSVATVSGSDSRSSASAEPDPGPLPPGGPGGRLVRQHRRGSSICSESNFSATVVRFNSGLEDTATTDAAGMLTRRGHMSNLATAQDVGPPLHPAIRVKALIQAAQLGNIARVAFLLKHGVDVNAVEQQQQQQHGNPHGVRGVLAGAFTVGGAAGGRTALHYAALAGSFGVAQLLLYHGAFAGVRDGTGRTAYDLARRKGHDAVAQLLKDVLERRRDVVRGCAASETRATAVAVAPRPGHPPPVPPRTKQLRSSSAPASGGAIDGAASVAGVPNALQAADVRTGLLAPAEACGRAGAAPSVSQPLSSIIDVVRTSPVTCGDCAAHVAQSKPDMGSLTAAAGLDSTANDDVSVVQTSTGRSKEAASEVSNAQGRSLIDVVEQLQTARTVEAVTFNQGARGTKEGTRQTRRNGGLFACFFCNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDK3</accession>
    <name>A0ABQ5SDK3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">VaNZ11_012311</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA polymerase beta' chain family. RpoC1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02584">
        <property type="entry name" value="RNAP_II_Rpb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02733">
        <property type="entry name" value="RNAP_II_RPB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000684">
        <property type="entry name" value="RNA_pol_II_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007075">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007073">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038593">
        <property type="entry name" value="RNA_pol_Rpb1_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF37">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04992">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04990">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05001">
        <property type="entry name" value="RNA_pol_Rpb1_R"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00115">
        <property type="entry name" value="RNA_POL_II_REPEAT"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="18">
        <location>
            <begin position="220"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1471"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1520"/>
            <end position="1856"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="671"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="920"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1526"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="1735"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1779"/>
            <end position="1789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1839"/>
            <end position="1856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="GLI67975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1856" mass="203767" checksum="D63415E747C8552D" modified="2025-10-08" version="1">MASVDRFSYSSAPVRQVKAVQFSVWDPAEIKAYSVAEIITSETYEKGKPKLGGLSDPRMGTMDRGGICTTDGANTNDSPGYFGHIALARAVYHIGFIKTVIRVLRCVSYHTSRLLVDKDDPKYAYGLRISDSEKRLRYFVHLCQGKKMDEATGNPQPSYRLDGLKIMAEFPKIKGDEEQEQDNVERKQELTAARALEILKRIPVEDCRALGFDCRFTRPDWMIIQNLPVPPPPVRPSVMMDSSSRCEDDLTHKLAEILRTNNAIKKQDATGTPQHVIAEQIMALQYHITTYFDNSSPGIPKSNQKSGRPIKSISERLKGKSGRIRGNLMGKRVDFSARTVITGDPNIGIDELGVPWSIALNLTFPETVTPFNIEKLQKLVDHGPNPPPGETGAKHIIREDGRRINLSVLKSGADRRLEPGDKVERHMTNGDLVLFNRQPSLHKMSMMGHRVRILPYSTFRLNLSVTTPYNADFDGDEMNMHVAQTHETRAEMRELMMVPRNIVSPQANKPVMGIVQDSLLGTRLMTKRDIFIEKDVFMNTIMGIEDWDGTVPMPTILKPKPLWTGKQIFSMFIPDVNLKNKSAWYKDTDVPDMSVDDSQVLIRSGELLTGALCKKTVGNGAGGLVHLTWLEHGPHAARGFINNVQRTVNYWLLQHGMSIGIGDTVADAKTMTKVNEIIEEAKRKVKEVVEKYQQNALEAQPGRTIHESFEAQVNQVLNKCRDDAGKAAYMSVDLANNIIKMVTAGSKGSNINISQMMGCVGQQNVEGKRIPFGFQNRTLPHFNKDDLGPEARGFVGNSYLRGLTPQEFFFHAMGGREGLIDTAVKTASTGYIQRRLVKAMEDMVVRYGGTVRNSQGDVVQFLYGEDGMDAVRIESQTFEYLKWDPVKVDKVYRVDTSRDMPPDWLSGDEYESLRTDPESEQALREEMAQLKEDLRVLREEVLTTGDEKVNIPLNLSRLIWNAQTKFGCKPHRPGWTGLPVKEVVVKVQDLCRRLVVVVGSDGLSVEAQRNATIMFHSMVRMHLATKRVLSEYKLNQKAFDWLLGEIETRFFLALAHPGECVGTVAAQSLGEPTTQMTLNTFHFAGVSAKNVTLGVPRLTELINLAKNIKTPTLTVHLLPGLRENKDRAKEVQAKLEYTCFRNVVKTTEIWYDPVDPSNPNQTIIGEDEEMLSSYYDLAGEEDFQVLPRLSHWLLRFEMNRGTMLDKGLTLGAVQKALQDEWEDFINVLVNDDNAEKLIMRIRVIKTEEASKDESADPTEDTMLKLSAAIMDVRLHGVPNIRKVFIRAENQHIYDRERGMFRKYEEWILDTEGINLEQVLAFEGVDPRRTMSNSIIEVLEVLGIEAARAALLKEVRNVISFDGSYVNYRHLACLVDIMTCKGCLMAITRHGINRNGNGPLTQCSFEETVDILYRAAMFGERDRLTGVSDNIMMGNMCPLGTGAFDLLLDESALESAFDVQVGNLVFGDDGLLNTPGRYTPGRSPHRTPGPMSPSHMLSPMHSPFIGGGAFSPIVGGGFSPVHGSGGPTSPTYSPSSPGYSPTSPGYSPTSPAYSPTSPAYSPTSPAYSPTSPAYSPTSPAYSPTSPAYSPTSPAYSPTSPAYSPTSPAYSPTSPGYSPTSPAYSPTSPAYSPTSPAYSPTSPAYSPTSPAYSPTSPAYSPTSPTYSPTSPTYSPTSPSHSPSNPTSPAFSPTSPDYSPTSPNYSPTSPNYSPTSPQYSPTSPQYSPTSPQYSPTAGAGGETGGAGTGGGAEPASRTGADSRKQAAYSPTTDISPVYSPTADVSPSYSPTADVSPAQGGPSPSPPAGPGGNQPYSPTSAHFSPGLVVNLSPPDGQGRQGDGSYSPQRTSPTFSPSFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDL1</accession>
    <name>A0ABQ5SDL1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012163</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Magnesium transporter that may mediate the influx of magnesium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF31">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="552"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="385"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="583" mass="63384" checksum="0CAFA3C82924D46F" modified="2025-10-08" version="1">MSTPATPLGDRLSRRSRYEALVSKYEEAAAVVRTHADTDVQLQPQDQPIAEEEPDPQQLQLQLQRSPPSSPRPLEIEAETHTRSIRLSLGGVPSMAPLSPGMLMRRGSSAVAGGGTGGGAPCDDYMPTAAGMEAGGYEVSGTGGAPQEGDVDLEAGGGGNNGSAQVGLHLTTSGVVSPFAAAAVVQDTVAGNGPHRKHRSEKTKKVPLVSTHWLKIDFMGRDTIIRVDKHKLMHKLGIQARDLRLLDLTSATPPAILGRDKAIIVNLWYLKTIITLDYCLVVSPDSIVDNEQERQAVAAFVRELKAKLHPVSAGQKVKSYVGLSSAAASAGYGSLQLPFELKVLEVCLDMTASKLDFNGKMLEADAYPSLDSLSHKVNPIDLEKARRIKNRLVRLTREVESVREILERFLNDDGDMHRLHLTGAEMSRQVSMRPGDLSRLSGGLLPMGSVPPDDSDTSSDSSIDEAETAAVEMLLEAYFMQIDHTYNRLQNLDEYIKDTEDLVTIKLDQHRNQLITIDLMLTSFTTVLNVMTVVGGYFGMNLNSKLQEVAHIFKAVVLSTTLGGLVLFVGFLAFLHQQKLLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEH9</accession>
    <name>A0ABQ5SEH9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012165</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052201">
        <property type="entry name" value="LRR-containing_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24111:SF0">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24111">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="64"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="616" mass="63587" checksum="752A2A66CD1F6EB4" modified="2025-10-08" version="1">MIKCKNWNSRSSSPGLTASSRKSACHPKGFRSAHTLFRSDGFYSCFFIARAASDSTSIDGKAVSEATRTTAGSGEGTPLGARSRARHRLTQATEPPQTASVPDSRTDAGIPPLAVAVAAVAGLGVLAVLYKTFFVNVGSQAKSAVEALPQTVAQGLQQKRLQRDAQARLNEMCEQLRALPSVDLSAKNLGDEGTAYVVEALAFNTSCRALDLSKNGISGGLGAAALAQVLPGAALSTLVLNTNSLGDGGAEQLAKVIAGHPHLATLDLGQNGIGDAGAAALAEAIKLNTRLQRLDLSGNAIDKDGAAALAAALAASTTLEVLVLTDNYLGEAGALSLGDALRANNSLKELHLKGNEIGDAGVAAICEALMARHGDITVLDLGNNSLTEAGAAVLTRMLSLKRSLKELNLYMNDIGDSGMAKMAHAIADNKGLAVLDVGGNNIGPKGVAALVGALRNHGSLTSLELGYNPLGPEGTKTVTDLAKFNLPKLSTLKMGWCKVAGGDGARALSDLLLLNNTLSHVDLRGNALGNDGAILLSRGLKAAENSKIVDLDLGYNEIKDDGACALAQALKANPEGAPKELKLNSNYITRFGQVALSEAVDMVYEMGKGKMTTVHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEN3</accession>
    <name>A0ABQ5SEN3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BACK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_012464</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051481">
        <property type="entry name" value="BTB-POZ/Galectin-3-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410:SF23">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410">
        <property type="entry name" value="HL07962P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BACK" evidence="2">
        <location>
            <begin position="251"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="744"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="785" mass="82189" checksum="59DB07CF2EB24D19" modified="2025-10-08" version="1">MKSVGNEHVAKQLASLYSSEEDSDCVVVFCLKASSLQDASATTTGKPLLKRQRVGESSISFNNGGRGDVIIVGDALPGHQLVLRFASEHFGSQLKQWDAKKSRNGTCEQSGPAAATVDGRPVLRVNLSHEGEEPSARTAIRYAYTGDVQTASIREALEVRRQAVNLRIKGCAAACMEIVKAKLVEATNTAVINNYMAVMDGDASPTRGGSGVGTSAMGTAVTRSQPAPLPRLPSHPVLELYACESLWPDLAHEPEFESILKSAKPQLVHHFDNVLQTLNTRSLCQQFLQLPPTAVEVLLESDDFGTDSESSVLLLLATWMEANHATTDIRTCERLCRQVRLLQLSRPYLSSILPVLALDFETCTIGGPPQPTIAAAGAASGAPVPSLTANTASTVAGAANAGLNNGPAACSSSSVAAAAAPAPAAWFPVRGAEAAFICSLATAQPPLRDALLAAGRKLYDMTLPWYSLRRRRQCLPATGLVYHWRIAEQDLAQALATLQPGGLCCTTGTFRGDSDFNYVVAGGFEWWPSIEYRHGAAEANLGLYSRLPVVYQVPGSRFTGPKPAVAIVAGVDVDLSVHHWHHEAFVDDGVYFSTSKDIWPVGGGRGANVLALRQLQPLTSAAATDVCSKGAGGGFSGVASLAAWSEYVRDGEVAGKLTLLQPSIEHRHGAAEANLGLHSRLPGVYQVPGSCFTGAKPALATVAGANANLSVPLWNRPGFMDDGAGTSKAACPAGGSCGANVLTSRQLPPQPTSAATADVRSTKGTGGGSSGVAPLAAWSTCGIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEV1</accession>
    <name>A0ABQ5SEV1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012331</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF1">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22534">
        <property type="entry name" value="RFC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="33"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="355" mass="40467" checksum="18758A0DB0161BFF" modified="2025-10-08" version="1">MLWVDKYRPQTFDKFVIHKEIGDNLKKLVTTGDFPHTLFYGPPGAGKKTLVLALLRAIYGPGVEKLRVETKPWQIELPSRKLEVELTTISSAYHLELNPSDVGNNDRYVVQEIIKDMARNRPLGVDGNKGFKVLVLNEVDRLSKEAQQGLRRTMEKYSSACRIVMLCNNVSKVMDPVRSRCLCVRVAAPTNDQMMEVLSWVATKENIILPPIFAARLVDFSARNLRRALLALEVCKVERYPFGDDQEPTRPDWELYIAEIAKNIMDEQSPKQLFLVRGKLYELLANCVPPELIMRQLLMELLKKMDDEVKVETVQQAAVFEQRLQEGAKAIFHLEAFVAKVMSIVKNYMVSCGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG01</accession>
    <name>A0ABQ5SG01_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamine-rich protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="273"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="916"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="994"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="936"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="954"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1006"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1062" mass="110523" checksum="D88416D5124D70D7" modified="2025-10-08" version="1">MSDYLRLSTVQVVQAIDLAELDKTIKWLVSKVQELPTADQANQRTIADSLLQDQIDALRRENSNLKDVLAQLQMKQDDMESRIDTKQDRDQAPAEDTGPSEAAAIDPLAALESRLATLEQRVEQLPSASDEAEAADAAPAGSDLNSQFQELLAGHRALAATVSGLQHELESKPNRVELDLKANRSEIDALLRAGVSVATVGDNLPPALSIKADGSVDNTALVEAVNKAAADVAAMRGQVAVIQDRLQGKAEQGGVDDLASQLAALKSRQQSSIQKSASASGGAMGGDDSGVPAQPGSGTAPGATADGLSSVSSLAALGSSPVLDSITKRLDDLEVRVSVAASTASPAASNNEHAVDAIGSVQDIQPLLKDIYARVDAKADRSALEDALRRLQEMATSLGQKADTAALTESTTHLERMISSHLSAHADKPGSARRKQEQQSHDLQWIVSHEVPADGDGYGSATLEKLSTQLSELAARVSQLPDAWPPSRKSSMGMEGLDLSSVQGALAALHGQLGELGTRLAGKADAGEVARLELALNSKAEVDELAEIRLALASKADAAALGDLQVAVAGKADQAALDEVKLTAALAADLAAGSNGDLNANGNTAGGGGYTNLLGAIQGMVADKANKAELGVLQTQLMNKASSEDVGLLRAALEDKASAAELSALMAQYSGRPANSEEIASLDQRMGDVFAELAKIRADMAALPTDTFGATAAAASAARDVGDQAGLQRIIAALNREVGMLREGLDTVAHAANVLAVGLDAGVRTSATGAATGGVEGRYSEAEGAAGKEHRSPRGGYERLLKLLGSGDYRHKMEAFDPVALQQMAQKLAYLEATVKSPAMGRAGLGGVDYGIKELERQVKRLNTDVRLMRDKLIEPAATLAISGSRIPPGDHAMLAARPITGYRCMACDRPLDQLDVSPGPHVPTQQLPVRVPAATDTTSRSQVRSGRGDPLSPQGSSQRLVYSNEPNGRNAQNWYKDTSAVIAAEALPRDYVGPHLPPGGWRPSNPGTNGKQPATQGSLPALSAPKTRAPPTGVEYEPRGQPVAASPMEGAAANITLPQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGE3</accession>
    <name>A0ABQ5SGE3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013393</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF29">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25764">
        <property type="entry name" value="KIF21A_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="7">
        <location>
            <begin position="11"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="252"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="623"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="739"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="809"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1025"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1120"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1563"/>
            <end position="1656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1668"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1715"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1947"/>
            <end position="2031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2081"/>
            <end position="2208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2366"/>
            <end position="2445"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="882"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1417"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="828"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="840"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1029"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1128"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1163"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1571"/>
            <end position="1594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1595"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1633"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1679"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1716"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1748"/>
            <end position="1757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1782"/>
            <end position="1798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1799"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1852"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1955"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1993"/>
            <end position="2027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2094"/>
            <end position="2113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="2124"/>
            <end position="2136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="2156"/>
            <end position="2173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2394"/>
            <end position="2415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2426"/>
            <end position="2445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="88"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="2468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2468" mass="257201" checksum="B62BA7795B809DFB" modified="2025-10-08" version="1" fragment="single">MDDTALQDAAAVRVAVRVRPLITRELLQGSRTCVSTDGPCITIGRDRRFTFDHAFGPSTAQEHVYHELVGPLVESCFDGFNVTVLAYGQTGSGKTFTMGTGEWGGDEDQMGIAPRVIRHVYDCVEARKHLTSFHVRAQFLEIYNEDVKDLLVQPTDQAIKWTSREQTPGPQASGGGAGSGITLRDAGDGNILVVGAVEESAHCAEDLLELLERGMALRATSSTCVNEQSSRSHAILTIIIEQHLYSNSSSQQHINSDRCSQSTLGRSPECSVGGLGSHDNMSHDGHSGGGSGGGAVAVEIRTAKLHLVDLAGSERTKQLRTSMNGTRFRETVNINQGLLALGNVISALGDERRRGSHVPYRESKLTRLLQDSLGGNSRTAMIACVSPADDVLEETLNTLKYANRARNIRNKPVINRGLPASEMLILGGAVQQMQLQVLQQLVTQLPDGALARGLPLAELLALDVTDPAACLDVLGRMKSVLAVCGASSRATSMDGVALGRQLLAASLASPRNRNGDASLGGQTSDTASREMEAVVSGCSRAIAGILHGLAEMEAQGKVNREAREHILRSLDASTLRLLPVVRSLGPRTSQAQAPASPLGASALARAAAGSPASWEVDGSGALSGNASVGGNEEASVAPGTTVPGSGSGSGTSERELELEERIRCLEADLSNCRAELREARDDLERDEIIFADKMKELQEAQAASANMKQRIDEAEERHSKEVEELRRQLVVLQTAVSEAQAVASSRSGGGTPYRQASRGRSRRGSGSGSGSGYLPMVRRRSSSVLLTALRVGAGEGDGAAAAAAAAAPGTAVTGGGEEGANGARRTEGGGGEGGGSNGDGEGEGEGERAAEEDEVLYDEELLSYISETDYTEQHRREVLESDAALREELAEVLREKAAAEAERAALERDALAQRSSFDEARAKLEQQLRWLSGSIGVQQQQLQAAQAAEREARELAAKWQERAQELEAAIEAREEALRKLRSELEAVESNAARSVEERAALRQQYEQRIAAVVAQVSALHRQLQQHAAASDRRERQHSTERTAALESELQRMRSQQAELRRQLSERVSRYERESNARLRELASLRKAATSARSRLTALENENRAQRLLLRAKQQQVLAAEQRLRESQRNGSNAAAIASARSRSSSPGSRNYLTRGGGGAAPRSNVSSAESSHDGSTQHQPGQRGPGQIAGARSAAAMTGIAGGNNRVKSHSPVRRTPQAQGHDAQVQRPSASSLKRLEDIAAEELAPLRKPSGNEAPGGTDAKQQDGPLEALAPEEQVELTEWAEQMLGLVGEVAGVEARVELLTSRHKDLLSRRELLLREQAQFALRTRRRAEQAAQAVAACEVELEDLAARAAAVATERRAAGVSAEEETLPWKENGPGGSHNKEWVGLLQQRMAEVRHARDLLQGRLRSGRLLDEREQQVLDSLDDQLDDLETQLSYVTGELADRRGLLSKLQGRREQMQQRSRGMDTAGLRLALAAATEAVGQRAFQVRQLEAALAASEAGAARREAQLQYRLRESLVSLSAHLASLEAASPSSSACLDSSELRNLLGIYLDMEIAATSGPQGGSSGSAPASAAGLKGWQQQQQQQQQQQVSSTVPGGQQLATTAPTAGHHFSSRSGAAASAWRPTEGANGGPGSDPDGGGSEGQEEGPQQLRDLEEPQLPEAESLAVWGSKPSQQQQQQQAQQRKCSPAPDELAREGLAPDLSLQALPASRNPTSAAAWPSSSDVGRQQQQQQQPQRDGASLDTREQKEIPPDSRLAVPFLARTASPRILESMENGARGGGGRGGGGGGGSVSEGGAVSSDAKPSAPGQQRPSSSSSSSQHLRSGSVSHGGTAPPSPTGGILRRAGSLDSVGSSSLGSTTKRVTWSSHLATGPCNTAAAAALSGGGSGAMRGKEKSILDRASGSMWAAPAAAAQRGNFNTNSSGGGHSAGGGAVFAGGGASEGVAASRGGGRENMRREVSGGSWLDRRLANAAMEDDDGGGKVRNCGSAGGGSSGGDPDAGGGGGGGGGGSRSSDGGGGGGASSRRAVLVVPGCPPESLSRTSGHSDDTVAASDSGVVLEMAASADLGAKAGLRSFNPWRGSSSSISDTKQPQIQQQQKPQQPQTQQQLSTANGLRGGDAGDHFRERRGGEGGDDDLLATQGSPPDDGGDSPSGGGDGGGDGGNGNGHTNGHSSPPGPAGDLSELKNQQLPLLWQDPPTRLGVTAAAPGSGACGGQGDGSGASLPGGAAVAVNNGVEVGGRAGCGTEYGSSDDELEEHVRLLELAVSAAAAVPEARLQPPPAQQHGHLSVPAFSPMPTARHWAGDSVPTGTTSTFGRAVVFGDQASESDVFYVSLSEDAASEGPAIAVEESHSMPIGVATFKAPPPSARRLSLDRPSAATSTTAGARQGRSGSSAPLPLPAPARSVSPLPHSFIRQQRQKAAWPGTAPAPGPAAFAASKTAGGMTALSEALLPYKTHLKRTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGM3</accession>
    <name>A0ABQ5SGM3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS4 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_013346</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI68924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="271" mass="31095" checksum="F6E82220BA1E9E4F" modified="2025-10-08" version="1">MRQLKHHEQKLLKKVDFLRWKNDDNIREVQVMRRYHIQGRDDYKKYNKLCGMVLKLTNVLKQLDPKDTARIELTDQLLEKLHSMGVLPMKKSLVQCEKLSTSAFCRRRLAVVMVRIKMAETLREAVTFIEQACWVRICAATKLSQDVAGASVSACFPFCASSVFTKILQARAHVAMCGWRLATLQGQLVIGKRARYKYPNAADAIADALHYCCANSRLAQGHVRVGPDTVTDPAFHVTRSMEDFVTWVDTSKVKRKVLKYNDKLDDYDLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGN8</accession>
    <name>A0ABQ5SGN8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="360" mass="38919" checksum="B9D1335A0B839199" modified="2025-10-08" version="1" fragment="single">AMTRIVQYLPRAVTPRFRKAYHSVGTHPAGSKALDLLTGGGYPAGSIIEASESVILRSHASLAVPSEKTRGFTFRSKPIVSLCGSQIQGSDLKPCWQLAWLAAAAAYKRGWKVTACSAAACLDFGAERGLTARDKFARTSVAQPQALLDVVRSVERDCSLIMVDSWARIAEAAAAPAGPDSSAIRAMRNRRLAGFLRQLSSRLLLHKAQEDNIDNDNGCMIGARSQGYVSRNWPAPSVIVCSTGQKGHGSVLQHYATMRLRVSEHREYDTAEDGPGHMVVEMLKGPAVNTRMPITQTRVYLSSRTTDVLSFTDVQDPCAALDLLMGTEGLGLVRKMPCNVSSLKNSMSVAKQTAEHDVYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGS6</accession>
    <name>A0ABQ5SGS6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_013736</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd15532">
        <property type="entry name" value="PHD2_CHD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 9" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DDT" evidence="14">
        <location>
            <begin position="159"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="432"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="1280"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="1479"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="12">
        <location>
            <begin position="1773"/>
            <end position="1843"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="12">
        <location>
            <begin position="1965"/>
            <end position="2037"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="12">
        <location>
            <begin position="2139"/>
            <end position="2209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="262"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="556"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="786"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="833"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1388"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1638"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1890"/>
            <end position="1946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2229"/>
            <end position="2307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
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            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
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            <begin position="556"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
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            <begin position="806"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="881"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
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            <begin position="959"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1424"/>
            <end position="1441"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1454"/>
            <end position="1470"/>
        </location>
    </feature>
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        <location>
            <begin position="1643"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1660"/>
            <end position="1673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1709"/>
            <end position="1719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="2245"/>
            <end position="2254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="2255"/>
            <end position="2264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="2275"/>
            <end position="2290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI69167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2307" mass="244939" checksum="BF3CCED66FCB0C3C" modified="2025-10-08" version="1">MSFDDVGRDRSLGGLQGGHGRLLRERVGPTALLGNPNYNQSLNSNGSLSSSSDSDDIEDFNAESREGVRGRGRGRGRGRGRGRGRERAGARRPSTGMRSSILPAMPCEEESDSLDVDRIGPIGWDDDIAPSPEGHINLDTPVTVTIEQARNATSLQVSGVAFADIISAYNILRSFSWQLKLSPFSLSDVCIAMCSGKPGRLLDELHVCVLRTLASHETSAARRRRRLDLEHLDQVTWPEFVWEWLELTGEKVLTKHRAQVLPPPPVKKEQQAKGEVLSTSGSAEEARNGAGGSQYNGVPPVLKQEPDQTAQQDPNYTVQWGTGRDGHRRPAGPGYKAQLLAQSLRQRRPRRDVFRSDFHNMPLDCNAAVLARLCDDLLEVPAIRTEINRREAAGQMTSGLRGEGGAYPQLTDADRRARERRMQTNAFDDGNSENCVLCGVGGSLICCDHCPAAYHLRCIAQTAHSIPDGDWSCPECAVGGRGESAGLRVPITGLSLETGNPTWVAYGYAFPAQCLPVPRPLPAPGASSLALLLPPDLEFRGFRLLTGEDAQRDVGGLRRPRRWPEDMPHPSSTHDLMEGPRSGCKAGPADPNAYDNRYRSAWQTYVQAMKAAHEVEYKKSKRAGATPASALAVPLPLPNFSWPAFQGRSIVSVPCGMCRSCTTSLKPGRPCLKPLLMDDSASLASELPQNGRLAVLQHYLLRTEQELWGLLDGGWALSASRGLEYRRQWMRAVTEAETPEQLAPRFLELEAALRKSLPIFTLQWDPYRRDPTELAVLENMAAERRRDADSYRRRRITSMLPPPKKGPGRPPRKKISPHLQAWNELRSAQAAAARASAVVATPQDDGGAEGASEMADEGGESEAVGRLRDIDGEGGDEDGLTEAGDGPSQSGQGGGTPMEDFQQTQQRRQRRSSTQPRGQDSDNEGAEGTEGPASAGPSSGRGGDGGSAQRSSSRRRQRGGGSGEGGGSGGGAGVMAGGRAGSVAAMGGQPGDQDMPIRPPGMEELVLDRWWRELLASYGEVDAVDLLGAAAAMGGESLFGPDRRVAIRKARTGWSWWRPITVRAEGYLMLPRAQARSLARKAGVVPLRQGVIYHTNLRHNMSQRLNWIAVTENASSVAQLAVQARILEDSIQWARIRRPSPGVDDSAMPASLPPEYAPYANYIVLAKRQVEPERRVEYLLAPEGGAEAVVQAHAQPPGWQQKQLGGFKILTAAAGLSDWVEESSVPLWLLRAFEERLRNAEYRQHLTGGTAAAAPVLLEGVCGGTPDALAGAGGAGSSSNSCCLVCGLSRSDSAFAALTAGGGWVSCAGAFGSCSTGCHAACLGTAEPGNRSKWMCQRCANEMLTQRQAAVDKYVARRTARGAMAVSLTPRQLLEQALSDAAAAAAGLNPDGGELPPLGGAATRADSEEGDGDGEMGEMGGRSGRGRGRGRGRGRWGRGRGRGAAVAAADGADADGRRGEEAEAEQRRGPLEGGGDGNELYCICRSRWEPGMDVMCCDTCEGWFHPRCCGLTPETAVSAAADGWTCPLCEAIASGAPHKMMAPGMQPQLQPQPQSQSQTMLQMSQGSQHLSPEGLRMQQLEGDPAALRHVGIPGESGVASGMHLGVADTGGKTQGTACRFAAIVLARARQGLTDMGGIERVWRGGGGWRGGGRGGRGRGGRGTGPGLGPGGARGKSRGGRRTTYDSEEGDDSDGGGSSGGISEEMEQSEPPPPVQPAPPARTAHFAGTSGGGVIREPVAVSRHESGGTQAANAVDAEPLTWRPSALTILRTLLLEPFAREFSAPIDLEQNPGYLEVVSRPMDLSTVLGNLRKGAYSSCIAVATDVALIAANCRRYIPPGSPTLRRAEQLMAAFAAAWRASGLPLPPSAGFPSDGALQDFEDGAASISDGAAAAAAPLQRRPPRQGAGLNTRLPGRGNDDADDDGVAGRTVSRPAARKPAAMQEGAAVPPYESWQPLALSILRSLLSDPRASPFAEPVDVNQLPGYTKIVSRPIDLGTIVRDLETGRYASLGPSVLLDDVRLVWSNCRRYNQPGSSIVADAEHCRSTFNRNWQRLAVPAGLPAATAGAAKLIAQQAASAAAQQERYEWPGGVTAAPPRRGATAAAAAAAASRGVGSGRDAGGSAGDDWHTAADEALQRLMRNRVAALFLKPVDPAEAPGYLDVVTRPMDLSTVTSRLRQRSYADLSELLADVEQIWENCRTYNNKSSRVYKLGEQAAAQWQTEMEAVGLGVGGAKQQRSRRRRRTTAAATMYDSGSDGGDCGGEGLDSDDDEERGGGGGGGRRVGGSGRAAGGYRASKRSRVGDFDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGT0</accession>
    <name>A0ABQ5SGT0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="815"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1085" mass="116788" checksum="E8F5B1DC5FD29101" modified="2025-10-08" version="1">MDAERWTSRWEPSGTGKMRPTGHETERGPSINRSSSSSWQTSYADREERWTKSDSSSTCVSRTHGRPSGGRDGGNSGNQWDDSRQHHGRYQPQPRRHQRSTTGPGANWVGRAKQEGVYDLETDLMGDAFGGLDPGASEAGRPTSGADGGHEVDPWSTGCIPNNDIGGRPPTCSTHLTLAQLEAAVSDGDVRSWSLERLAEAATAMCQLRSTSSPGQECYSDICPFSPREETVMDAIATAVIYSARHEAQLGTTAATATQIGWAVAQLLWACARGQYWCRPPKPQPGTWAGSAASAATAAAAVATVSLYGTGPAANGNGVGPGLWPSPAELRSHDAAEPVARASPVALAEGRIATDLAGEAYHPEESGGRNRGSGAGAGSREAGEGSSAESTRDSVESISVSHGKPLAVELMHWLSADGCRIVRHLDMRSLSLVWWALAKAAAGARARYSGTHLQPPATAAADILAVISPALVAALDARAIEELRQKNNAKLCALLYSCACVNKSSDQLLQALGDELAARLEKAIVLAEVRIDGTQRLRFSAREMSCIAYSCGLLGLRHEKLMAALSEYAALSSHTNWSASAISVFMYGIANVGHSDQRLMAWADIAISAKPALEFNLRNISFLLYNFHRLRWWPQRAAEHLLSATRLHAPTTIPLISISTILMCMGSGRARDERSLAHLCQALTQRPPSQLSCKDICICLTALQRLHGEDVLASQQPEVLAALAHLVDAIIFRGFSRPTPACRAVPVFNHQEVSNAMFALGKLKYYSPAVLDALRDHAARGGLEGASAWDCTAILVGLAQLNYGGPEDGDRGTCGARRSQAGAQGARGRSHGAAAGSGEEADVDLVYVLADTVARRARTAKSQDLANTLWALTVLEGSLGRYRSTVTTLLDEVNRRGPAGKNPDGFNVDEQRQLWQVHRELGALPQPHPSLREDLVRLGKLGMSEADHTYSRVQASFQSLLNAMYSSGRYPRLKDLLFEQELVPGGEQLMRIDVQAIIERPCPGGGVEEHRVAVEFDGPFHFMVNEPFTTRIDGRTALRNRILTRYLGQENLVCVSMAEWVAASREPPINGLPGRWRLMASKMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGV1</accession>
    <name>A0ABQ5SGV1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF18">
        <property type="entry name" value="ORC1-LIKE AAA ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="796" mass="78306" checksum="098C17EF8CF07653" modified="2025-10-08" version="1" fragment="single">GAPLPPVPRVRLGTRRYIVVVEAPHVPLVHHTSAAAGGSGGGRSGHQLLLSPEQLVVEALARRPHTIRRCDLAATAADAALSAALVEAHMRRLEAQEQHQQQQQQQQQHQRRRQQQQQPQRHDAPGPRAHSTGSSAFLCEPIAAAADEVTKAKENARRVLSDLTRSTPPASASAAAAAVAAAAAINGLVSPSRTSTTLPRASGGGVRPWASGHSGRGGYTLAAAGGASSDGGGGIMEVAGDVVAAEAEAAAVAEAEAAGLAQALPVPEPQRPVATVRGPEVSAGTSSVADAAVEAAEAAGGAAAAGASSSAAATATSTAAAATTSFLNYFIFRTTSARGMSLGGAPATTNATAAAAPAAPVVTAPAVASKSVTSTDLASGTAPAAETPSSRTDGSSLPPSQKNTPSDDRSVQPQPQPPMMPSASATPAAAAAAAAAVAGGLGGTAAAAASQPARDSDSGSAAGLHHNVQPAVLLSALLQDVQEKCGLLHLGLRPVYGCSESSGRSGSGSGSGSGDGGCGNGISGAHQGLGEEGEGCGLVARWREEVMAVVEPGPEADRILDELGNCGGSGDGSGGGGGGGGSRSLASLFAAANGAALESSRCSSYRLLARQEVWHNGTALATLLLQVTRPAALLRPCDFAVMAPQLSGLVVTAAAPLPTAVTTALLRAGAAAVICPYVSTAAPQVSRGQGGAGGTAAATAAAQKSCVVLPYSADGGAQQRGDKVEQAGQPQPQRSLLAASGGRSEGTSALGMGDAMVGCFEGLVSELEEGATVLAAIAAADRQHPALRGRIAFHHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHB1</accession>
    <name>A0ABQ5SHB1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="500" mass="52117" checksum="AC5A3C46B33D7D17" modified="2025-10-08" version="1" fragment="single">MSNYVKQQAPAKRQSLLPNSERTGKALFGSYAVRYSKASQSTGGSKGSLTAESSDLGVEQDPGAGATSSTGSAAANGVSLPNSAAPGASKLTLATSALSTASTQTDQENVVPGTPPTPPPPCTVPTKLVDAVDMPTGCAALRSLLPGPPPAPPPVVVKYSSSVSGTSAIRELVQATGNGIMDALSTGLGTATKGRGPVKPVTTWRTRSYNRNMESEVTLKLKLKSYPIVAVQVVFTDRATLPNVIRIMHSGSHNGPFRQLGSDMPLAADGEPRLKQIYLLGATHVPVQQYLRITLVGHLSFPAAGVHDVRTLEVLVPSPALPLSVVVPGAGSADTLGTLSPPAPSMRVLQGAPLVYRVTSPLPSPLQPILNERYTSPAVAKELPARPRYEQQTSSAIESNRSDAREKGWKLQTRLVETASTRAHAAAQLEAQQQLQQMQQQLYQPIMKGQSYPAPSGGPARARAHPDAFSRSLMGLGGSAAARQPSTSSKEATIGSGNIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHV9</accession>
    <name>A0ABQ5SHV9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008637">
        <property type="entry name" value="HR_lesion"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05514">
        <property type="entry name" value="HR_lesion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="153" mass="16905" checksum="54FC4AE69294F36F" modified="2025-10-08" version="1">MAAISNTFPLVLARFMLACPFLIASVTTFALLRDKATTGYVIEENKALIRALGDFGKQVPQDKLAIFLAVLQGMGGFLMVFNHKFGGLLLTLYLLPMTLLTQQFWKEEHISLGLQQEALVSFLKNFGLLGGILMFMATTDGVPLKVKRKAHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHY6</accession>
    <name>A0ABQ5SHY6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_013855</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="11">
        <location>
            <begin position="268"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="299"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="613"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="106"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="453"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="787"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="879"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="268"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="482"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="518"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="903"/>
            <end position="914"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="914" mass="98522" checksum="582BF95334337D01" modified="2025-10-08" version="1">MDDDLELNIAVPVTPKTRPSKPSKTASSGKDGGAKRRGDSTKAAPTPATGNQANGKILKSKTSGFQLRDSNLPNTCQRHSAGHDKKWPGTAKLGLKSLKQLNSVNEKLNRVQHRPGKERFKRSAGGGGTDPDDPEELMRAPEPEEEQEDDLRAVMSLMAQREKALRQEEQQAAAARGGKGARVPAAEREGVVEFDDDDGDGGFRKRRRLNGLADGGGGGGSGGAAASVPKPKGRGNYIPGSLVHAVANATAAGAGGANGAAASSGGGSEWLELGLAASLGEDLTTLGFVSPTPIQRAVLPVLMAGRDALVKAQTGSGKTLSYLLPIINNLQAQQPRITRADGTFALMLAPTRELCIQVYDVAAILLRRYHWLVPGLLIGGENRQHEKARLRKGMAVLVASPGRLLDHLESTAAFKTSELRWLVLDEADRLLDLGFEDKLRRITEQLNKRSGRGIGAAAGAAEQQRRKRQRQKQNDQEIERVDENEDDQEEEGMPGGRSGGREEEDGRKQRIQCSEASAGRHDGARSEGGDQSSRRQTVLLSATLHKQLEVLAELSLQEPAVVGFEVRMTGEGLKLRAAGVAGGCGVEDDDPLSRYSLPASLRQSWMEVPAKERLVALAALLRSRVARRRVGGSKVVVFVASCDEVEFLHHLLGDMWEASAGAQLLPPSVRLFKLHGDMPQAERTETFAGFSQEGDGVLLCTDVAARGLDFPNVTTIIQYDVPGAPSEYVHRVGRSARMGAAGEAVLLLMPHEIPYVNLLRSRGVLLEQVQLDKLARWMPAPQPDQVRLVKGKSSNSRDSGGASGGAARQLAQQLHRHVSMAVSHDPHAAKLANNAYRSFVRAYATHSGDVKRVFAVRNLHLGHVAHSFCLIETPTRLGSSASAADRKRRKWEAAATQKEERRRKMKRAAQRVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI22</accession>
    <name>A0ABQ5SI22_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013607</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010635">
        <property type="entry name" value="Heparan_SO4-6-sulfoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12812">
        <property type="entry name" value="HEPARAN SULFATE 6-O-SULFOTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12812:SF0">
        <property type="entry name" value="HEPARAN-SULFATE 6-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047287977" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="26"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="36618" checksum="14C63FFCFCE6A330" modified="2025-10-08" version="1">MSRPRILFVLFSILLQFSCWKGGEGARGDFCRPELPGCCPKVVDHVLLLHIEKTGGTSLRTFLQTAVKRRFQQGAICNYLRHMPYQCLPSSEGGGNTSCNARNFRYYEEAISTGSHFKRSCKLTSSHHDFRIIDALPPHVRNTTLLIVNLREPASRAVSHFYMLRRHQDPGAVNSTILDYFLGNPVGVSVSRNRMTRVLAGEFCCKDGAPVLYDKRELLELALARLDQFCVVGLTSRVQDTMLYVQHALGLGTERTPKNLHYHNNAKKYEPVDELVLQRMREANVLDVELFKEAEQRFNLQMKSIGRGGFGGTGNSDGPQGSQQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIN2</accession>
    <name>A0ABQ5SIN2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="173"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="330" mass="34857" checksum="B9EED25AD613E9E6" modified="2025-10-08" version="1" fragment="single">TQPAVAKGAAHAMHALTRSTAAKPHRVQAALSRPTICDKCRSSLAVNRSMTTSISNNNDRLCSISRDLSDVRSRWLPDQPTFVCPPSMAQGSPSPSDQAWAERGIFLSYGKVNLSYKEVERLLQLANLDINPSDFRSATEVVWAYRYQNTLASSEGSGTLLASDQEGRSCGAVELIGFAAAEVAPPGPLPSKNRGPVAEQHWRKRQSAADTFGAEGHDVCISVTAEAAGIGRAAPSVTAGPGGSSEKTTVATVWFLAVHPALRGQGLGRALLERIVSELRAGDNGDGRGGASLVTLRAVSRATAFYDRLGLMRDPALPLVRAGRDKSGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIU8</accession>
    <name>A0ABQ5SIU8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4203 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="41819" checksum="F60373AA59FE5A61" modified="2025-10-08" version="1">MAHSFNPLTLTMWAIVVLSFLCVADTHMGASFGPPAQKRSDGVSGLLLSRILKIWSPTNLKSTSSGLPGTPNNLNLPSIREAVIDPPLEQQPSRCAIGLPSGCGVELPISVTKSTGYIGAWKSAVSTAGQLFEKCVIKKALDEVNFRMVGTVLAGCALVLYANWMSKSMAFRVTGGGLVVGLMMGTIIFCWLRRTLRLGGCSVIGAIVGMSAWMAGFWRLPSLTFLITNTYLLACVGFFWFIGSASVYVGGAGMNNPRLETLLALSFRLLGHVLLYIGLWKHETLALCVQMLALLYCVLPTSLRRFFWRTVQFGFEDTFQDPHAPLRLNCGPHPLVVNGFIWNRLMVAIPIDGPEFRELLAQGYEPNVYSGKMELVSGSEYPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIX4</accession>
    <name>A0ABQ5SIX4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014644</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019399">
        <property type="entry name" value="Parkin_co-regulated_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207:SF2">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN PARK2 COREGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10274">
        <property type="entry name" value="ParcG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="309" mass="34286" checksum="2DCA16762EF1226D" modified="2025-10-08" version="1">MNGLPDVAGSLFTSTYRNVKLAGKAPPATNLTGTGSCFDTTSLSPARAGAHKLLDVGKEELPVWSKSTLSYKYPSAKPNPGGFLKKGDGDLIKTRTGQVEEVKPLPPSAGAYKRRENPPNTAFRRFYERGDLPIAVDHRGSKNMIAWKVDIEKLDYHHYLPIFFDGIRETQEPYRFLAVKGVEDMLRVGGSKILPVIPQLIIPIKTALNTRDHSVMCITLQLLQKLVLSADLVGEALVPYYRQILPIFNLYKNKNKNLGDGIDYGQKNYDCLGELIADTLALFEQKGGDDAFINIKYMVPTYESSVNYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJH1</accession>
    <name>A0ABQ5SJH1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="597"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="822"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="853"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1080"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="613"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="860"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1093"/>
            <end position="1111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1135" mass="120606" checksum="693F97523B7F07A2" modified="2025-10-08" version="1">MTHHYKHMFDTRLVFPKDLLVHGQGLRPCSMEGPSQAAPRLGPRAGYGAPSNAPQSEDHSLPLPLPLPLPRPRPGGPLRASSRGPWAPKRPKISPLPGTLMPTQLQLQHMQMRLRLTPPPSPPQQQRQQSSPRLHPSLPPQRPKHLPAEHSVVPLSRPSSASPAPRPPALMDGSLAHHAATHVANLLLQQAARRPPFNLSQQHQPPHVYPPRRTALTRAQLDAHHLPYRTMPYNHIARTTSDPAFAYSYNAALHELPVEGAMHSKEDGALMHGAASDPSRCCPAPPPRDHCGGEFDDGSDGNVKVDGDWYADSYSDPGLQLTQTHSPHRWRLERQLRTEGLGPCSGIENGSFTGIWMSQRPLATSSLGTSLGYPGVTPAHITRMAAAAAAPLSPRRALEEAASAGVDTEVVGLHSAVPPNHVVRGRSRPPVPQAPLRLRDLRQVRPMAAAMTPRPAVGSNILGDDIASTADGFSERQYDSVRYLNVDYDRGMGHGGRYGLAGYRGGSKAVHQTLPTDFDREEPQDDLRVRSTAQYGTNVSSPLHDTGLNGSPGSAAGMVYDHPPHLHWQPAHPKGVSCGAVSPPAALLIRKRPSATAGLSNPREFACNTGRKGRMEGSPDRPETTGATKAGLCHNAGSSSDVSATRCTVQRPDDHGTAAAAAACPGASATTAATVVTVAMASTNACNDRNESSSALMGLLPQRDPQSTIGFLPTPINGPAAVAATAFGSNTSLADYAAAAAADANGAGARLAEPTPLGISIPMRPRRTIAAPGLLSTAKVDAALTAGVAAPDTPSSTRYAGSGNIRGPGAAAAAAVTAADSAPDVSDGSLDGGSAPPDQFRTNLPAAVTAAEIKPGIDSRPSTTGAGAQPFKTSSQAESPVASSPSAYVRGLDSGPGTDAVGIHPMADDVDAGMQLLAERFRTRPGRVPKAMAQLGPIAAFARDAATGRVQLGRTELHQLVACVETKLAEVVSEQKEGNRVRNRMAQRMHRNRQRERIQQLESKVSGHKLMIGQLVYAVQAYQAQLRKLCEQEGRIWNDSPGLQTLLAAAQAQLQQAPCPPGRGKWVQAQTLQQQQQLTTKTHEQPLQLEGWPQQPHEPQQQEDIPPTQQQQEEHGCADEQGLPLPYTKQRHQHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJK8</accession>
    <name>A0ABQ5SJK8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="135" mass="14362" checksum="8CF9CA03CDF1260C" modified="2025-10-08" version="1" fragment="single">LSYCWCCRQRMLAAKAAAEALKPVPSAESPVKGSASCGPPAGYCQIDPGVLLPLHATTVDPLTARLAPGQELRLSGRQVAPPPVRSGVVLGWWQCVVKVMRLFGDVCDNPGHHHPHMGAVSIPLTKRAHDGSVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK95</accession>
    <name>A0ABQ5SK95_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014820</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051481">
        <property type="entry name" value="BTB-POZ/Galectin-3-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410:SF23">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410">
        <property type="entry name" value="HL07962P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="188"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="100"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="528"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="461"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="681" mass="71927" checksum="73AAC7F1A0F25902" modified="2025-10-08" version="1" fragment="single">SQYLSYRIGTYVCDNQFQPLNVAKTFPWKCCSCNSHIRGPNMERLDFSNLWENERWSDLTLVIKPRCCGVGDEGNAVDDVCPSSSTPAPPENGRRVTRSMLRGAPAGPGRQQSKAGPQPSTLPMAHPARRRAASENETAKASRGARIAQGSLGKSLRSAQLSVQAQAEADARNTQPAGAGKAGTLDDLPTGSVMYPAHAAVLMSTSEYFRRYFDAWHPTDGSKIVTIEVEPDEVEATKMMLRYMYTGQLPLQGVDPSQLLRMIKLADQFSVNRFLATCNSALADVPLDSLTLDHVASILALPPFLLEDGEGPGGGGAGALVVARRRAEAKLTAVFGDLEGAWFDPIRRQEFLRLPLEAVRFLIRRTETAVYNENTTLVALTDWIAANSHSAGQANRLNRHVNRRNSNGGVDGSGNNAIGNAEVEGAGGAAARATGTSDAGVAVTVHEVAAGTLHPPVPGTPRGGGGGGGGSGSALTEAQMDELFGLIRLPYLSAAFMSLLPHVGWISRRLTPQLLARVLQLVKADVGPPGGGGGSGGAGGGPRGVGGDGRPLGGMYSNGALDLLQRLTGPVAVPRAMPERTVLELEWTIHRSDVQALIAECQAEECVGVECGGGCGGSSGPGVCGSGCDEVTAVESTRLFSEPAYYYAGFYWRMCFFVQKKLEPKRRRLGDMQEVSGAGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKD0</accession>
    <name>A0ABQ5SKD0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FACT complex subunit SSRP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_014854</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the FACT complex, a stable heterodimer of SPT16 and SSRP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the SSRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01390">
        <property type="entry name" value="HMG-box_NHP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.220">
        <property type="entry name" value="Structure-specific recognition protein (SSRP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035417">
        <property type="entry name" value="SSRP1/POB3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038167">
        <property type="entry name" value="SSRP1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17292">
        <property type="entry name" value="POB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="13">
        <location>
            <begin position="609"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="10">
        <location>
            <begin position="609"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="457"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="679"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="486"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="506"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="536"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="587"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI70081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="700" mass="75589" checksum="0199D126DE5BF345" modified="2025-10-08" version="1">MANVMAEGIQAFGEIWLGSRGGVSSGQLRLMPAGFVWRRKQGGKNVEVKKEEIEGLSWTKVPRGCQLSVRRRGATTLNFLGFRDKDLDSLQQYALNALELSGGIAVEALSTSGHNWGAVELRGASLAFMVGGKVAFELPLADVCTAQQTKDDVMLELHVDDTGVEVAEDMLQELAFYVPPGNDEFPARGDDVPASKVLLDALLPHADTEAAAADEPVCVFSEVGILAPRGRFDIEMHLGYLQLGGQSQDFKVRYTSIQRIFILPKQNTPHTLVVISLDPPIRKGQTYYAHLLCQFPTEDDISIELDISEEALTAKNEKNGGKLSTEMSGPVHEVFAKLLRGLSGARITKPGHFRNAAGDGFSVRCSYKADDGQLYPLDRGFFYVHKPPLLIPDNDIESVEFARQGSGGAMSSSVRTFDLVIRLKGGTEHMFRNIQRSEWGNLFEFINTKKIPIENLASAKRGPGGAHAGVEAEDDMDPGMRRAAAEADDDEDDEDEDFDASGGSESSEDEDEASDAEMIEEEGVSADAFKSKGKRKDSGSADAEERPAKKPAKEKTVTKIESAGGSDAAGGSGGSKIAAKGKAKKGVKGEGAEAGKPKKEKKVKDPNAPKKNLTAFMYFSNSNRDKVKAENPGIAFGEVGKMLGERWKGMSLDEKAPYEQMAAKDKVRYAEAMKAYKAKGGGVPFSKDDREGSDAGGDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKD2</accession>
    <name>A0ABQ5SKD2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015355</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050595">
        <property type="entry name" value="Bact_response_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591:SF3">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44591">
        <property type="entry name" value="STRESS RESPONSE REGULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="13"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="246" mass="26234" checksum="3C42EE3EC5866A02" modified="2025-10-08" version="1">MKPCHFDKHGRLRILAIDDDSVNLMVIESVLKPTGWSVVPAIDGSEAYAVCDDEDTWPDLVLLDYNLEVGDSGEVVLAKLRSRFGSANVPIIMCTAMSANSVELERCLSSGAVDILFKPYERDRVVDMVQKHCPGKLASPAPRQPALAPVPAPAAALAPAPVPTPATATVRAPAPVAIQPPPPPSAIEPDVESFCTGLGLEYVGKKLKEQGVTLKDMKALDDPGLRKLGVVVKSQRDKILEAAKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL25</accession>
    <name>A0ABQ5SL25_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015636</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06227">
        <property type="entry name" value="M14-CPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034269">
        <property type="entry name" value="At5g42320_M14_CPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF119">
        <property type="entry name" value="OS02G0119300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="6">
        <location>
            <begin position="56"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="372"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="377"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="413"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="424"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="466"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="553" mass="59715" checksum="484E98DA1BBF9D50" modified="2025-10-08" version="1">MGAAPALGCTAVMGQIFHLAVILLLLLRTQSVFAVRRRLASATDSLATFASPNYTIYHRHGELISEVVEVVNANPGFMKLEVFSERDGSYDVELKVVTVEPGGFSNRHGEKVRMLLDFGEHGRELISSELGLLLLKTLAAPEGVMRVFPGELDRGRELQRLLDGTVIKILPMENEGGRQLVEAGRLCERKNGRGVDPNRNWEVDWGTKEPDYDPNEEYPGVAPFSEPETKVLRQVAAEFKPHVWLNIHSGMEAMLAPWDHLPEIAVEARPSVDILQRIFFDIMANTTCVVGSGGKTVGYLAHGTATDYMFKILQVPMAYTWEIYGDLKAHFDDCFRMFNPLDEEDFKEVLQRWLRAIMRLVELMPGHPALKDSKAWSVPSSGSGPGVQQQGHSGAAKEPGDGAAPGTTLQQGEGQGNVQGGGTAPITAAGATTTTISSSSKTSSEKSSTSSTSSTFTSSFNTAGTQQEDSGSAKNQATEASRNPSDNGTASGDEPRVAVVASVNEWRGTWSTGFTSGLVAAAGLGVVGVLVYRCLSRKGEEPRRVRGSGYQPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL36</accession>
    <name>A0ABQ5SL36_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">VaNZ11_014805</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The aspartyl protease (PR) mediates the proteolytic cleavages of the Gag and Gag-Pol polyproteins after assembly of the VLP.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000091">
        <property type="protein sequence ID" value="GLI70178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0229">DNA integration</keyword>
    <keyword evidence="16" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="14" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-1188">Viral release from host cell</keyword>
    <keyword evidence="15" id="KW-0917">Virion maturation</keyword>
    <keyword evidence="18" id="KW-0862">Zinc</keyword>
    <keyword evidence="18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="20">
        <location>
            <begin position="222"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="21">
        <location>
            <begin position="526"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="238"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="757"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="280"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="780"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="802"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="832"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="855"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="GLI70178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1400" mass="152007" checksum="7B41E9051805DB82" modified="2025-10-08" version="1">MGKDKLAEPLDLNNYSTWAYRFENLCAEHNLNRALLTEPVTDEEVAASERVKTLLVKNVKDCHLPVIKASPNAKMAWDGLAAIFAANNHARRSQLRQEYTKLAMKKGEALPVYLARVRELHTDLLKVGQATSEQDMTYAMLTGLPQKYEVIVSILTTQSGELTLSSATAQLLAFEARVGSMHEASTSSSAFVSAGRGSSATGSAPRRGGSHVRGGSREPLQCWNCGKPGHLERGCTAPRMAQRSGSWSSAPRGPGYGSGGLERTRMGGHGEAAGGPSLGSDRRGYGRHDHVDGAGDRGNASPRNFAFVASGSDRLKGWYLDSGASHHLTYDADQIVDVRELAESEKFEILSVNGGIIRPTAVGTLRLIPGVNMHARVTVEEVYLVPDSFANVLSVAALDKQGGSVCFEDGQVTMRKGGEKILTGQRLGDLYALSCFSIPRSASRLRYTQPFGGVAHGKSASESMDLWHRRYGHLGLGNLERLVGEGMVSGVRFAKEQVKGAGDAGVCEPCVMSRQRRGPFPSTGHKVDQPLALIHLDVCGPMPERTPTGSLYLTVLLDDYTGFSAVAFTPTKEAVKDKVMTMVNQLETYSGFRVKEVRSDQGGEFINSPLQEFFRTKGIRHGPTVGYAPEQNGAAERLNRTLLEKMRALLAESQLPQCMWAEAVSTANYLQNISPVSGRPCTPYEAFSGEHPDVSHLRVFGCTAYVHIPKEKRNKLDPVSRKGVLVGYGDAGQYRILFSDNVVSVHSDVTFDERNVGVKAQQASRAKKSVRWRNPVDSTPDSDSDSDDDDDNDEWEPAPHTQGGGSSTGAGGSGDNSGGPTGGGDASGAGPSGSSSGAGPSVGAAGASNNFESRYPVRDRKRPSEWWRSEDPRSVAGRVHVAAATKVSEPNTYAEAVSGAQASEWKRAMDDEINSQLANGTWTLETPPPGTKLIPCRWVYKVKHNPDGSVERFKARLVAKGFVQREGVDYGELFAPTSRSSSFRVLLAVAAARKMPIHQLDVTTAFLNGELKEDLWMEQPPGYESETPGLACHLHKSIYGLKQAPRCWYDKLCEKFAEIGLFPSKSDPAMFLKVDERGVVVALVHVDDTCVASDDLELVRKVKEAIGQCFKIRDLGEARVFLGMDIRREKDGDIVLSQSRYIEQILERHNMLDAKDRATPLAMGTRAAPVTDDEGKLLEDPTPYRALVGELNYLAVSTRPDLAYALSVLSRSMAKPTRAAMSAAKGVLRYLAGTREMGLRFPGGQGLQLDGYSDSDWAGDTVTRRSTTGYVFRVNGTAISWSSQLQRTVAASSVEAEYQALSSAVREALWLSKLSDDLKIGTSPSIIKVDSQGAMNISNNPMTSQRSKHIDVMHHLVRERVASGEVELRYCPTEKMVADVMTKALGEAKFKWCRASMGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL59</accession>
    <name>A0ABQ5SL59_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="274" mass="28581" checksum="D36FCBEF1D2DBBB2" modified="2025-10-08" version="1" fragment="single">FPPPSPQPPLPPPPVRSPPPPPSPPPSPPPPPRPPHPPPPLPPPPPPPPPRPPSPQPPLPPPPTPPPPPPPSPPPPSPPPPSPPPPPPPSAPPPLPALPPPLPSLRSPSPRSPPPPFPLPLPPPLPPSQPLPPPPPPPPPPPPPPPSPPPPLWPPPPSPQPPSPPQPPSPPPPFLPLPPSQPLPPPPPPPPPPPQSPPYRPSSPPRPPAPRRPPRRPFPPRPPRPPQKPPPPAPPDIPPPVNIEAPPMDIQPPPEYEVSPPPSPRPPRKKVPKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLB3</accession>
    <name>A0ABQ5SLB3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="159" mass="17907" checksum="A94160425E556257" modified="2025-10-08" version="1">MMVLATNAACIHQKEEIQATRRRQAKSQQPSKARNSSRNATNSSKAAAKKVTNDGDVRPSTKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRQKKVKSASEDVRCTRARRQRGGSGSKKKQELFEEEVSKDERSEDEQDDATSAREDDDNDDSEIEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLI7</accession>
    <name>A0ABQ5SLI7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012416">
        <property type="entry name" value="CBP60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31713">
        <property type="entry name" value="OS02G0177800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31713:SF96">
        <property type="entry name" value="OS02G0562300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="556"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="620"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="786"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="574"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="798"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="844" mass="88962" checksum="65E1AF16F8425071" modified="2025-10-08" version="1">MEPPVPFPAELRPYRATFDQYLDAKLRQFGHVLLRQISNSLTADQIRSIIDEAMVTMHQLEAAVAPQRPLRLVIRHDCQSSGCGDQSCRLCQFNPSRLCTRNLKPKYLIDDHLKAKCSAQLRVELVDDTGGCHTEGLPTGVKLELQVLNGERYKEICPDNVLLSVSAIKSCIINGHQKALLRREGMADTDLQVSLPLERGVANLSELTLTTSSEALLNGKAPTFRLLVWAIQGNGEPVPYVTYVVSESFVVATKRVKHAIKSDIPSVMDSVSKLVHIGKATVDKLIDLRAAFQEEGFDFKLPEDLIKVERVGQFKKLIDLSESNNDLKNRLRHVLKLSPEKWEEVCQHAISAVVPDFRNRVWWCPPLRLGLLFACKNGAVMMENPTGIVKLGPTPDARSQVLQLHQLSPSDFQALPRLKANALQNWYITNHPNWAIFNDTGEEGMLQASPAPLNVGAAGQSNGGTAGLTMVPLETLGGVPPRSAYAMAGGPPSPALGGLAPAGGGGGTVYGREMFGQHGIAYGQTDAGAYGSGAPGGLGDMPIPITGLSPLPVQVPGGHRVSNSSPFAMPQPPQQLQQQTQPQQSPIAATGGVGVKVQVLSPQTAVGPGPAPTGQVFRDHGASMPPAASGSGSGTSDGLIGGQAVGMMGTAQLQPPAASGPGSSTSNGAARSPFAADAMMMSIVASFPTFPDMRRNQHSPAGHGGQLTLQSPTDPHGTTTHHLGDGGHGQQAGSMFSMDMLNTDVLVNSLVTNDTTDWTQQLHGIVGQASMLAGINSYTMVDVAPPGDGGELLESGDGAHGGGQGQAGASGGGPVSHGDGCGGLVGLESERRLSCKLNSMTLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLP0</accession>
    <name>A0ABQ5SLP0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="562"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="654"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="654"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="692"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1032" mass="108247" checksum="96597C1264EACAA7" modified="2025-10-08" version="1">MGCASSAARADEQTSASVKKQETATSKDQVEIKSTVLSLQESGFALGLTKLINEATSGAKPKDTWLILYKCTDLICSTFGIKTCSIVLYTSTGLSCKLTCNTGSVLNELVLQHPDLLPAETLGFTTSATANQETVCVEAGDELWDSLGRESYADFHLLTGSRGACTALAVLPLCGGPKCPGALLLGHTAPLAPWISQQRWIMMLAPLLGLYVSEACMVRKLSVVEKLVDAASLGALAYAVTTELGEVFEWCHREDVEARLVVLGQEAETATVYCKVPVVTLVDDRNSMRSGISSSLARSGPNALLEDGLVGTHMSLDNTLTKRCLNGRQRLLFVADVMQALKLYGEPWKDIFFDNAVVLTPSWVLAAPLILEGKKLGAVLWLSSCRVNSDVLMHTASTCASPIIHAITRAVAMHQLYAAAGSGPVAMVGGAVLGDSAYSKGTLGGGGGGGGGGGTSQHGVSSFTALSSRQSSTLSRGRTQRLGTGPHEEGLAGRGSNDHEMIVGDLDVGGSRPGVSAPDIPVKYILRHSPSTSALMRMYKSTIAQRAPAVDDDRITGSIHEIRILSKAGEGAFGSVYVGRWRNIVVAVKIIKDTCGTRSLKTAWELAVNKSLSHPSIVTVHAILTDVHLQKTSTRILRFIPAATLESQQVAAAATSTGPGSPSHTAFAFTPSPRLSNPDGAPVNVAATPRPLTGNGSTSASGGPAAPVPASPVLATTPSCPLPGKDSAPAALAALGGGTSTVAAAAAASGGGGATGGQDHSALSGNVAPVLPPSVFGRMSAGSNTGLLSAALLKPTKPPTPKVHAILMEYCNLGGLHKYIDNRMFFKDRTMPEKGSDAARGLAPLPPESVHMDFILATLSEVASALQYLHTQGFVHCDLKPENVLLKEANNRRGFTAKLADFGLSELRSPDGQVVGDLGGTVTHVAPESVLHRQVSSASDMYAFGILMWELYTGQQPYRELLSHISKREDRHRALLVRVVHEGLRPSFPCGVPLDYLSLASQCWSAEPSARPTTGEVLQVLEVMYTKYAGAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMQ9</accession>
    <name>A0ABQ5SMQ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015193</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="526"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1381"/>
            <end position="1408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1517" mass="168958" checksum="9DFA4A036160E193" modified="2025-10-08" version="1">MAVDFRQRYVNGLSSGWMLYVCRKPTTLPSSTNACLNGILRYATKQIHTQNWKIAAYSWSNSTHTAGVLILIRQGLASALAPKLATPQHTCTRGRFTDCILHWGGHRLHICNIYLPPGTTSMAAETRRQLFENALAATTTQAMEDNRDTTLIWAGDFNFVENPEWDSSAGRDGRQADRPITEMRQRAGPQMVDTHRYLHPRRRDFTHLYHAPTPGGSRLDRIYINEAKLPYLCHTAISFHTASDHRLATLHLLPCKNEVKGPGIKRIRMAFTDHEDLATSMAEWIRNQCQEAPSVEENPGNLIAWWPGFKRRLTFLAHQLNKVAQQRRQQPGAHTQEAYLGVQQAQQALDTMPHSPHLLQQLIDAQARLARVHIEDNARVAKKTRVHWLHAGERPGPALTTFLQTQIQDQQTAPVALQAHSGRLVTNPAQLPQVVADFWAGVCRAPDFTTPEDRQSVLTQLAAANIRLPEEADEVAGSPTIAVTELGFALKKSQPGKAPGWDGIPTDLYRKFWPEIGHTMAALFTAIGKTGQVPTGFLDGIIKVLYKKGDPKQPGNYRPITLLCSDYRLLAKVLANRLGPALGRIISPEQSAFLPKRLIGASVLFLRHLPHLLRKLNRQGLLAFLDFAKAYDTVDRNFLMGAMEAMGAGPQLCSWTRILLSNTRARAMVNGFKSRLVKMEAGVRQGCPLAPLLYLFVAQALFSWLKSQEIGLRLLPSDEDRVTACQFADDAEVALEGEESVPAFLEAMDTFARASGQRLNLDKMEILPIGAVDDHHQGSTINGLKVVTTATALNLPMDNQLQPPQPDWPEMLALAYTRMQRLAHYPLSAFGRAAAVSNYCLQTVTWHMEHGGPPPDTTLTYIERLAARLVDRKQGPEDQGQRFTGVPSRLLVGHPSTGGFGLLPLRQHIRARQAMWAVRFIRGALMARRERERVMLLDTAMNTVAEEEEEPVTSGNVRSLHPWIRTLATYLEITHPAFKPWALTTAQATGPWLGGETLPEDISRIVGALGHLPPLEDVSNPPLAPGDWCWAAALWGNPLLPNNSDNGGSINGNNHRPGLETRHPLMASCRKLLTVGDLVRVYDMLEHDQIPLPQYMTWLDWTQQFLDPSASPTQLRDKESTRMALSALCNDISPAWIAAARKVVNRLQPQQPDRVEDRMQQQMPAPPDRTTVMAMIVRRLGWRLPGDSGIVPLYNLTVSQATQLQGVSTAEERLELLSAFVQEAQGMPTDGPAPPCPVTNLQRFQATLDRLWTAVRWENKNKETLWRLVVDGIPIPGNTHLSRVPIKPCGCGSYGADTDQPCSPRLHHFWECPIAKAVREQIDRHLPLPTSATIEGGLTRQQLWLVQAPPGCEQAPWDVIALAALSSMEYGRHCLRAATREHRPQWPSTHRRRQQQQTHTAEQGPRQRAITDFFNRSGGFPQEEEEDANTHPIISTTQPAMDTPTQTATSPLDRAQARTVADFWARLHSFAALGIPHYGWDQVGPQHPILAIVGTQMRCADPVDITNHGAARTEQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNR4</accession>
    <name>A0ABQ5SNR4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1236"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="777"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1108"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1238"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1325"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1343"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1384"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1408"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1623" mass="167912" checksum="4D6203AFB1B94BE3" modified="2025-10-08" version="1" fragment="single">MLKAPGPIGSGAQHQACKRKGPNALLPGSASSVDRPSGVPSFELQASGDSCIKISACSGAYVTLPSSGCNVPLPKPTGTPDPWHPHLQPYVFGGSSCKDLNLGLPTTRLPQVLVEWGLRWQLLSPPRLPARPPPSGLVSRGDASHHHRIPASECTCLKSPPEVSGSGHRLRLNELICQYGLRWHGLAPKFIAAAATTTSTVAAVAAAGTSPIAVAETFRASPTCPFCPNVSMTSFAAAATITTTTVFSCNDGQRVRKRGQWRTRYQRIDDRDGNRDNGAGRGHPDRRHRTYEGQREKQSRGVEKQRHWRRRPSMAGGDRHGGGNLLVSLLTLLVLVASLLLPAAGSIGDVGNGVALMSSPAPSSQLAAQSDRMHLPTTPRAPRAPRPPRPSPPPRPPPRNPRNVPPPPSPAPSKLPPVTVPAPALLSHFSPLPPVTSPPPPIQQPLPLAPPSSPPPLPLVQPADFHIRSSSVEDSTIAGYDIWPANPSSTSPPPPPPNPEPLPPPSPAPPSPLPPSPPPPSPLPPTPPPLSYPAPPSPPPPSPLPPSPPPPSPLPPSPPPPSPLPPSPPPPSYPTPPSSPPPSPLSSSPPPPSYPAPLTLPPPFPLPSSPPPPSYPTSPSSPPPSPLSSSPPPPSYPPPPSSPPPSPLPSSPPPPSYPPPPSSPTPLPSLPPPSPPAPPSLPPPSPPAPPSLPPPSPPAPPSLPPPSPPPNPSSSPPSPSPALPPSPPSPRPPPSPPRPRGPRSPSPPPRPPRSPPPSSAPPSPPTPTPGRPRSHPTTPPPLPLPSTPPPPLPPPPNLPPPSPPPPFQSAPISPPTPALEPPTFLTAASPLVAPASSQLYDITSPKAASVNPQAESLKQAILLFPPTPSLPPPPPPSPPLPPDTAYDVPWDGPEDTRQGFIGETRLVQITFTLQSLTAVSSSESLRTFIIAVQDAILVAAGDESCTAFTDVESFDGVATLVVMSGVTCDSWVSAPDSTDGSLAALVSRPLEFFDATFCSRFGISNVVVKVEVDVPPPPPPLQPPPPPLQPLPPPLQPPRSEGLGPGPTTPAAPHSPKPRVELLPPTPLLNPPPPFLTAVSPPTPFKEPFANAPGSPASRISPGTSSSQPPPPSAAPLAPPAVLQGRNVRTAFLMESANAVLADEVIRQTFLQTVQDTISAAAAFATCIVLTVGQAAAGSRSVVVQSLVTFDPRVVPADNVDSGLVALRRQPLAFFNATFRNRFGVATITVTVSIQQAAPPPPPRRPAPPAPSPPPPPPPPPPPPSQWPLPQSPPPPPPPPPPPLYRLLLQPPTSQPEPFASQFPPMFVPEPDSLQPPTSPPARQPPLSQDSLQPPTSPPARQPPLSQDSLQPPTSPPSLQPPLSSADKDLLQPPSPPDSGSQQPLPPSLPEPYWLQPPPEWVDGHDPLYPPIVDWPPFTPWVPFTPSPPPPPPPPPPPMPPPPSSPPPPPHYPPSPSPTPPPPPPPPPPPSLPSSLMPPRPPNKASTPAPPTPPPPPPPPPPPPSSPRPPFPHPPGPSPPPPPLPPPPPPPPSYPASPSPPPPPPPPPPPLSPPPPLSPPPPLYSPSPSQPPPPPPPPLLPPPPPYPPSPSPPPPPPQPPPPPPPLPPPPPPSYPPSPSPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPX7</accession>
    <name>A0ABQ5SPX7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_016963</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S5A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01452">
        <property type="entry name" value="VWA_26S_proteasome_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027040">
        <property type="entry name" value="PSMD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="3">
        <location>
            <begin position="24"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="413" mass="41916" checksum="D5F349C645E01F58" modified="2025-10-08" version="1" fragment="single">SSLDRLSELPDVFRRIGNISAMECTVVCIDNSEFSRDGDYAPTRFQAQADAVNLLAGAKTQAHPEATVGVLTMSGKTPRVLVTPTPDLGKVLNCMTNIEIDGECNVSSSVQIAQLALKHRQNKNQRQRIVIFVCSPVTEDKEKLVKIAKKLKKNNVAVDVVSFGAEDENQEKLEAFLEAVNSSGNSHLVTVPPGPVLSDVLISSPIFQGEGGSGFGFAGGVGGAGGGASGADGFEFGVDPNMDPELALALRVSLEEERDRQNRAAAAAAAAAGAAQAEGAGAGVSASAAEGATAVAGIAGAAATPGPVAVPGVGAAGVSGDMDLDEDALLQQALAMSMEIDNAAAAAQEAAAAANDVTTPAPTAAPVVTPGAPQVAVAPPPADLDVMDEDAELQLALQLSMQDSGPDNKEDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMG0</accession>
    <name>A0ABQ5RMG0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000517</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="117" mass="12477" checksum="A5D5D016919F10C3" modified="2025-10-08" version="1" fragment="single">MIMQLAHRLACAAISTAPGCQGRVVAVYGRSFPASGRCVSSSPGSCAAMGSHMSDDDPQAIAHHKEKALKGEMPAFVPEVEGWHETLASVSEAVVKAERYVDVDATKDPRKLELLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RND0</accession>
    <name>A0ABQ5RND0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="168" mass="19177" checksum="A3B3B2E3276DC951" modified="2025-10-08" version="1">MQLAYIRRRRRTRPLDADRQRVHSPLRQGTPAGMLQTQARRQRKGTAHSSCLPVLVTNDNGHPVDVRPSTKVRRTSTGIKRKVPVEDKSTDGDHNNDSYDHQRKKVKSASEDVRCTRARLQRGSSGSKKKQELLEEEVSKDERSEDEQDDATSAREDDDNDDSEIEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPN6</accession>
    <name>A0ABQ5RPN6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein-serine/threonine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001246</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05467">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE PTC7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="2">
        <location>
            <begin position="73"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="1406"/>
            <end position="1744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="857"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1169"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="693"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="740"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="782"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1075"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1182"/>
            <end position="1191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1748" mass="177348" checksum="7CC63B5B92C78746" modified="2025-10-08" version="1">MTITRPNSSRVAHNERDIYRFNRLASVLRTRFTSCPNLGLQQQIVGQQQAKSRNFNKNAEVRCNLHRCQASTVITQETRIIKVRLCFHHQVPFGQVLKVVGSGPKLGDWRPEGAQELQWNQGHIWTGDIHLEPGSYEFKCIVTAGDGIVAWESGANRILQVPIGVRGIRVDGTWSRTVENRITVVEPIAAASVAARQKRVAAARLLAKALAEVSAAAAASGPSGASLAGQKQASGGTESEAGIVPTSGPAVTKSDKRSGADAFEEEALRGLLHSPELLGFSAGAEGAVTTRRLSDSGARAKGESASGSDRASDVEKDLRKAVPPASTAGKVTVIVDAESTRNSLNGEAAFLGAASTKEDYKDAEEGVKDHFGAAAIAAEQQQTATGRASNYNRLDVLSISAEKGEEQQGCQPEQQEDHERQPVSTLAALPSGAVLKAFLPAKEQPREQNLQRENPAAGAATLSGIAQRIREAAVARSEPLAVRDAATSLRGTDAAATDFKASTEQQVSPKAAALAEAEVAAVGAASEAPVEAVSVPLSAAAEGVPTSTAAACQELASPATCVPDLASPAISTAAVQSAPIPVVPVPSASAVLVQGNQFIESEAPSATGVVSGPKEVLAGAATERNQGTDIGKGGKLVAALTIEDGLQAVASAATAVGSSSSSSSSRARGVSWRQLLAQKEQDLQESTGAAEAAQRKRPEAREFNGAEPEVIQDPQGKADPQVKEEESVSSRFAQLLASRLSREGKRPEPETDTIDLANPVAPSTPPGRDSKVPATEGATATFAASSGVSEATSCGDNSSSNKKAVKGVTAEEMGLGSSREESRDSGVESVSDPGLDQLLGAWEAAWSKSRAKQHPASGAAAVLPNSDSSGRAGVAKDGATANGTNAWQQVVVTAVNAGTSGLAGGCSQSRLGSTPGAGAKIVNGGLAAGPASVQGGSSGESLYSIAERLTGGSGPMAPPIGSSDESSPGITVRLGAMAAMGVDRQGSYDTRSSYLSQSLLADNLSDSASQEQSSKALLRKLRAANTAAAGDSMGAAAAGSAPAATSSMPNLPATPAPAGILSSVDDDENGDIVPPRLPATPPPPSYLSNLPGVTSGTTSAAPVPPLPATPVASSILSGSLGGISSAAEDLIRRASDADAVLNMLRGNLPLTPVARGGRGVAGPLSMVEEEVGDKDDETRAGGRNRRNRRGAGGRLVRLPPIQGAPIDSQVDPMAAARAVAADAALALDRTRAALQGLGLTPEALSESATRLLQHLQQLEAPEVQSGTAASGEGNGAAAAPGLPPVVSQLATTLVSGIGNVGSSLTEQLRKAGTAAAATVIPGAAASGTDAPNGVVETKAAAPANGITASSGFVGDANGNRAATSALSVAAAVAPANAGGGMLGLLGNIVGGLMPVPAGCVRLVAGAHMIPHVDKVDKGGEDAFFISRVGLGGVGVADGVSGWADEGIDPAEYPRTLMRYAADAFEAARGTMTAQDIIRYAQYRTYLKGSSTVCMALMKPGNKLEVANVGDSGVRILRNGKVIFGTEAQQHAFNMPYQLSHPNNVEDPDSADDADVHLVDVQEGDVIMLATDGLFDNVFDTEIEQIVSQQLWELAATGRGKVPASAGTTVAAGTTSATNGVSEAAAAGAVCGNNSNGSSGSGGAGSRGGKGLLSQSFSAAAAAAAAAAAAGGGVPAFGNSWQYRPEDATRLARALAERAHLHARNPTQRTPWSVTSSQQPNFLWAKFFAKGGGKMDDCTVLVAFVCGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQG0</accession>
    <name>A0ABQ5RQG0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047401105" description="Pherophorin domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="25473" checksum="2EB670D6E75D8AAA" modified="2025-10-08" version="1">MRGYKMIPKCSFGALVFVAAAVLKAQAFNNHLCGNGVPCQCDTQILPFPEEITEDFQFEIHNLYTDRQRGARLNIFVKFRYYTPAIVSSYTTESGTFDYQDMKKVVLGFVPAGGMGIPHDTFWEVLNRDLAKAVWAQFPIRGLSIRLEVQGMSSGGIAGHRSSTVTVGNIEPWVSIINNFPKCDGMDTDVGNKACHVTPTWSDGDPESPTLAVIAANKTGTHDNPRTCDTCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQM4</accession>
    <name>A0ABQ5RQM4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001832</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052993">
        <property type="entry name" value="CFA-57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="148" mass="15418" checksum="C1E1AF73B5438B69" modified="2025-10-08" version="1" fragment="single">GRRRGGPGGDGKSSLDHCSLVRYSPTGALIAAAGGPGGRHVYIFSTLTKRQLAVLAGHYEAVLDVAWSDDGAYLATAGEGAVFTWAMDGFCKVQENTSKLYLNDAIACTPEFRTLVVGDTTQGLRIMETSRTTGQCGPGVAPEPDAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR58</accession>
    <name>A0ABQ5RR58_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16981">
        <property type="entry name" value="CID_RPRD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460:SF38">
        <property type="entry name" value="KINETOPLAST-ASSOCIATED PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="592"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="700"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="751" mass="78033" checksum="C332A4D4C725F511" modified="2025-10-08" version="1">MSGGGMNVNRESLVQRLQSLNVSQQSIESTSKWCLFYIKDAKSVVNIWLEEFGRTTAERRIAFLYLANHILQEGRKKGREFADEFFRVLPKALASVSRSSDSKLRNSAKRLVDIWDERKVFGSSQVRVLKEHLNGSGGGGTPTKPGSGGGIAAADGAPGSSSGGANNDMDRRKLTAVGSLADVLYEVANAAARSSEWQTKCLQIKANVSSIDASLPDVVAARTTLATALECLKAESERRKKAVTYIRMHLSQQEDSLKRTEDAHAALVAQKAELDERHAALLKQEQEQQEREQREREREREREREREREMNEAMKQQEQQQQQQFQQQQQQVSMGAMGTEGPAAFLGIPPGGMPGMPPMMPPPFPLPGMDPTVPPFGLPGMPPFPGALLGPGAPPGMNPLAALAGAPGMNPLGPLPPFLGPLPGMNLSMPHPPGMNSLGGGGGPPGGGMGQLPSGLPDLAGALSALSASGFGGSGGGDSGAAGNGPQPPQQQMLQPQGSGLQQQSSGGVMPQGPGQPPQGAPPPVGLQHPHAGPGQPPPQPSQGMGHPQPVAPPQHLPGGPGHQHTPGGPAGIGGPPPPSHLHLHPHPQASAGPGANSLAAAGPGAPGPITAVGPPPLDPAGIDQLAAALANNSDQTAMLLANAFASMSQEERDRLGANLGDMLGSGGGGGPGPHGLQPPGHPHPSHPPPQPHPSQQFQPHHHHHHHPSHHHPPQPQQPVDHGMGGGFGGSPMSNGDGDTMDAPYDPEFPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR87</accession>
    <name>A0ABQ5RR87_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">General transcription factor TFIIB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002027</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20551">
        <property type="entry name" value="CYCLIN_TFIIB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="7">
        <location>
            <begin position="85"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="451" mass="50342" checksum="286FB74C0D0AC14E" modified="2025-10-08" version="1" fragment="single">FLRTSLYRYRTDYRQVSLHIHPAYRFSDRKAGCLLEVRARAAATIPASPLKFRCLFNIPIVPPEVYISGITASGWRSQRRFFSMDNEPCVTCQGVDFVEFKSEGDLVCRNCGTVKESRLIDERSEWRTFSDKDKDTTDPNRVGGPTNHLLEGLTTTIGRERNDGGLSYTLNRIHSRTNNPDRVIMAAMKEVGHMCEMLKVNGVVKDRALEIYKEVVEAKSLKGRSVKALAASCLFWACRQEKQARTFKEIVAVLGNDVSKKEIGRCFKDLQQIKKDELQKQGASADALASTMNQTVQHPAEFVRNYCNQLRLEFKCKKLAEEIAQNAKPADRRVPWDGRTPTSIASAIVFIAMVLWEIKKEESQSQAAGSLPAFAGLKAAKARADAAMQKVAIISSVAEATIRAAYRDLFPHIAQLLPTPADKPEERITPEMVAKLPNPDSRGGGGSLIMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRL0</accession>
    <name>A0ABQ5RRL0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_001875</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033438">
        <property type="entry name" value="MOLO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33748:SF5">
        <property type="entry name" value="GROUND-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33748">
        <property type="entry name" value="PROTEIN CBG04600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17175">
        <property type="entry name" value="MOLO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046853375" description="RING-type domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="272"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="408"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="556"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="272"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="433"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="568"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="578"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="593" mass="63701" checksum="A9D0D7847B1275A9" modified="2025-10-08" version="1">MQLCRFLGLFVLLTSASARNWSPENYPNPMVDVESCGRRGVSSWVCDPDGILTYESANVVEGIIKKIYAGEPPYAVGPCGNLGPKGYQVAVALMRRMSYVGDAGEAAGQFAKSLHDRWGVGDAHCNNGVVLLVSINDRQIFVSAGSGTTSLLTYDVIGDVIDDVRPALKDRRYDEAVERAVVDIGLALAGRPLEPEGGKSTGWEEWLAFGIFGAAVSGIVFFGGRTELRKRKRYQDCKSKLEKLKRDQAAVRNNTYNPTSCPVCLEDFTDVPPGSGSPGDDTGSAGCFGGSGSGPAGDAVSGGGAMKEEEELRKPLLAPTSIAAGPKANEYPGGVEPSAPPLPTAAETQQPSLQIQPPPSQQQKQQQQPVPLVQRRPLVLPCGHAFCEGCITQWLEQKKVTCPVCRKPFDEDDSPPAGDPRDSANFPRPPRPDNSNRNPNTGTQPGPSSSTGRLSSATSSPPRPPCSVDDEGHDSYLGTGHGSGVSGTVPRLRLRWASRRRPVRMHGDLLIAEMMFRLRQLQRQHPDYISANMMHSWEQDLQNGREFSAQQLREFQLSDPATRRDLASRGSSGSSIRFGGGSTRGGGGRGGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRP5</accession>
    <name>A0ABQ5RRP5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vegetative cell wall protein gp1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="39534" checksum="CED0A4BC9E31B5FF" modified="2025-10-08" version="1" fragment="single">MEADNLRVGERGRRGEALWGCLPCWLRLQPSGTCLLLHTTSSPPAPQPALQPSSPPALKPSSPQALKPSGPPAPQPALQPSSPPALQPALQPSSPPALQLALQPPSQPSSPPASPPALQPSSPPAPQPALQPSSPPALQPSSQPSSPPASPPALQPALQPSSPPALQPALQPSSPPALRPSGPPASPPALQPSSPPASPPALQPSSQPSSPPASPPALQPALQPSSPPALRPPSQPSSPPASPPAPQPALQPSSQPSSPPALQPSGPPASRPALQPSSPPALQPSSPPASPPAPQPALQPSSQPSSPPALQPSGPPASPPALQPSSPPALQPSSPPALRPPSQPSSPPALQPSSPPALQPFSPPALQPSSPPASPPAPQPALQPSSQPSSPPALQPSSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSG9</accession>
    <name>A0ABQ5RSG9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5'-3' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001903</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038969">
        <property type="entry name" value="FEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42646:SF2">
        <property type="entry name" value="5'-3' EXONUCLEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42646">
        <property type="entry name" value="FLAP ENDONUCLEASE XNI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="5'-3' exonuclease" evidence="4">
        <location>
            <begin position="8"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="356" mass="37232" checksum="6A60A07B5CB75195" modified="2025-10-08" version="1" fragment="single">LGPERAEMLGLTEPAGVAGGPAAVATLEPPYKAGRASKGVEFYSDLSNLQRLLGVMSVTPLCRPWLEADDLAGLLVQQAVRKGMAVRILSGDRDLMQLVSDPWDVAMLYPAKSLPKSPYSMPPSTSPSGATSLSAATAAAASEGLAGTTPPPRRSRLRGISLAADFPFVKLRDADVVSALGVAPALVPDYKALTGDASDRLPGVAGIGPKTAVELLTLYGNLAGVHCAAAVRTSKLSSKRRSALQVHVCGRNLCCTCCHCGSTQCGHHLAALSSGDGPASLRPCMTDPMHRPGTRVRVYCLCVLNLCAPIWVPESGRGPSFVHRVLPPSPPCAPPFPPFPLPRTSVRTRCPPPCTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RST4</accession>
    <name>A0ABQ5RST4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_002778</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="9">
        <location>
            <begin position="99"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="18"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI60605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="285" mass="31670" checksum="81DEB4263FC40459" modified="2025-10-08" version="1" fragment="single">TENSLFRFTRGRTRHKNMAERGGERGSFGRGFGGRGDRGRGRGARRARGKKEDEEKWVPCTKLGRLVQQGKIKSLEQIYLFSMPVKEYQIVEYFLGSALKDEVMKIMPVQKQTRAGQRTRFKAFVVVGDHNGHIGLGVKCAKEVATAIRGAIIQAKMSVVPVRRGYWGNKIGKVHTVPTKVTGKCGSVTIRLTPAPRGAGIVAARTPKKVLQMAGIEDCYTCSRGSTKTLGNFVRATFHALSKTYGFLTPDLWRESKYTKAPFQEFTDFLAKPILGEAKAEAYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSW1</accession>
    <name>A0ABQ5RSW1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002548</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037">
        <property type="entry name" value="FORMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037:SF24">
        <property type="entry name" value="POLYCOMB PROTEIN PCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="392"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="565" mass="60117" checksum="84D060F804027B40" modified="2025-10-08" version="1">MEVSNGGDHRGSKSKPCRSLSSWLNIVAVTTLIALLNLSPRCATQLISTDIEVSPVEALPSKPPNDQNLGNVPVAPLQYPAGQQTFSPAEPLLDYPVTPSMPLIASVAAFTAFPTPRPSPRQPRSPPPSPSPSRYPRLPSPPAPRPQPPSPGPPGPQPPSPRPSKPSPPSPPFPPLLKEPRPPPPKPSPPSPRPPSPLPSPTIRRSPPPSPFPPPLPPSPLKRPPHPGPSPPPPFSSIPSPPLSSPPPCTTCVSFSLARPEWQLFEVNFSPDICGSLSENIIQLMYDMAANVNVDVLGVLSASCSDTLMKVCFTFASDEEGGKLQSQAEFFLYIFPGKLLEAFELQGCSAVIDGYVFSLIVGGDGNDPLTDTGCFQYFQSFSCAMPGGSGKPNCTCITQAGTTPFGAQKALTELPGRKTTTVLYCFETAAAFKMSSYHAVVNGNEYDFCNSTTLLRKAEIWADEAQRQAVKGIGVQPSGSPTMRFISPSWGAPGTNVVKANQLNWTLEQADGGRICIELVRGTDLSSFCNTVGRPAICWIYLFDPSGACCPSFPAGLMTAKPPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSX9</accession>
    <name>A0ABQ5RSX9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002874</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF232">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB3R-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="15"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="17"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="73"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="73"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="836"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1269"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="508"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="842"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1272"/>
            <end position="1333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1333" mass="135464" checksum="63225EDEA3323450" modified="2025-10-08" version="1">MSRRGGGTPRHPTAKHKGGWSPEEDATLVSLVNRLGEGQWSPIARELNEIMKKTESTGRIGKQCRERWNHHLSPGLRKDPWTSDEERKLVEAHRLLKNHWSGIARRIPGRSENAVKNHWHATLRKRVTPENPAGPLKAYLDTLDLTVAPRRRGAGGASARSGGCGSKRRRSCREPESSSPSTDIDEASGGDADDAEQEGQNPDSTGPGPSRGTARMRKSIPAAAPRHQHSKMAGSAAGKGAGSFGALGAVAAVAAAGFGQSAMKSEPLFLGPPGPSGAQKRFPSDNGTLANQEALSVAAAYGSIAATRGEIAPERAAAVGSGVRRRRSKRAAAQVGNERLRNLVEAEQREAAAEAAEDAEAAAAAAGGSVVQGDGVDPRRVALGSCPDTQSFETKAAAGAIAGAGAGGLEALPAMKLEASLLSQPSRTESSCDSGPSFSDNALREIAADVAEATDGPGASTGANHGAAAAATAVAAAAAAAVGMGGVDGPRGDLQLDPAPFPLGGAGTSTSTQGQNGSTSGLGTTTLRAGTTVMQAAAATEPKTFSHPDVATGAGTGAPPSGGKLLLIDRQDAAAVAAGNVGAGGGAAGWTNGVYSGSTNDVQGLADGQQSRRSSYGISLGGKVRPRFCPRQEVHTPLPGVDITGVANTVGDVGVGGTATAARGRGGGNQSGAGASPGISTPVPTLGTAASDGTPSVFLARTCGDDEDLLLDTPVDYSGTMCLRHVASGSALFDYDMRHGPEFANDLATPPSPTKAAAATVRRAADASASAAPSAANPSLPWTARSRYLSFPECALTPSCLFDVMMDFVPDSPRNQPGENLLPVPEPEGRASGTLTAAAAASGGGGGTAAGSGNASGGVGKGQAYTEMAPVNVGVPYGLIGGAGGAGSINAVRANSSITSVGSPLSDVYVENGESGVRVGGTSGAMSLHAGTAAAAAASTAAITAAAGGAAPSSGEGLADHPEALTKRESCINAPHQHGAVSTHPMAPFKIVVPPPMAVSATGLQPASPWTPAQGMLAAAPPLSMTQTMPAQPASPGHWAGHLPPGQASGASVHMQYGLSAGSAAALPAIPSLTSSSALSPQLLPQALPAQPSHMTVTATAANIAPQLQHCQQLPLPLNGLYEGASILQHPQHSAVMSQPFPLMPSIAPTTTLAQPLGQPPQCQQSGMETAQPQTFQPVQALQTQPPQHHALLHDYIMTPGCYYIPPGTLAPFGGVFNALPPFPPAPGHPPVALQMSVPMQIWHSYHYAPEKMEGQQYQHFQAMQGMMSLPQQQQQGRKEQLQPLSQWTSQQQQQLQMSMSQQQILQRSTSQQRLQPLPQSTSQQQIQLQEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTA6</accession>
    <name>A0ABQ5RTA6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003018</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46817">
        <property type="entry name" value="PHOSPHOINOSITIDE PHOSPHATASE SAC9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46817:SF1">
        <property type="entry name" value="SAC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="3">
        <location>
            <begin position="162"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="719"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="657"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="744"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="825"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1078"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1149"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1244"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="661"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="690"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="755"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="827"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1152"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1254"/>
            <end position="1273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1288" mass="137332" checksum="15076244AB336A9F" modified="2025-10-08" version="1">MSASSPSAPENGRVLLVHSTVKQRYHVVARGFGRCLTHVLRIAQHSGKLHFTKTKDQDIFDSEIAALAYLRALDGPIQILATAPYILGYVVIGPWGLVLVAEKFRVSAVLPGNHEVKTVTKSHWLKIPLQINLSMQSVQAPEAGAKDEKAMKDEIEKGIERLLSFPVDGAHFLCDTLDITRPFPSYRAIKDPSWDFVWNRWMSAPFRNLGLDHLCPPLLQGLCESRLLEDFDNAKYWVACISRRGCLHPGPRYKARGLNDYAEPGNELEVEQVIWRQQQRDGSLLLWSRYTWRRGSAPLWWGVSIKNNGIGEAEIKIRPHSTFKGSKRYVRRLQERYTPNPQLDPDPPVAGADGEPSRHVPVTFVSLLRKGLPDRDRSEAKLASAFDFVVAQLRKEHGMNLTYIALDWHEMDKQLGHMGIVEAFWNTVKDILPAHGFALGTLEKIGPDHTEVCFDKGVGATQTYDGTGIVGPRVSAAGVGWRAHWIRQQRGVTRYNCADSLDRTNVGSFFGAVQVLIEQCRELDIAIARTRPTSDQVALQLARGSRDPRTMMRPGTAGSNTAMSSVGGTVGGGLQLQVPVPSNSTPYAGNTGGSTSSSYGATALQTGTSNSFFPAGLSARGNTAGAMFEKIGKQVATGLTTMIQPLAVGDGRSPLRGAIASSCPSPSGTSTPTISSLGTVSPLHRPPSAPGQQLSQAGPSTAAPADALQRKLAIAPDGLPLPPGWEAKIDKTTNRIFYVDHNTRSTTWDRPPVPSSGRLSSESLSTTFQPGSGPSSHSLSMGPTAQGANSMHYYRGSAGLELRVASGSGNNPAGTAAALTPRGMVSGQAQESPGDVTQQVRAGPSGGGSHSRTDSGDAGSGPDDRFEPLTPWCMLRKGKVHTISRRIHPEALSVLAELFLVNGDMCAWLYTGSQAMHSERILIFEPENSKLRKVGVGAYGNALVAVKRRYNNVLVDKEKQQMIDVFLGYKQLEYFPSSWLAYRVMEKEGVPLEGDDVSDLDTEPVGQIDWPLPERNAAHHLHALYGAGQGQMPLPGGSLIMSVAGSTSVFGGFGGEQTPAGPSSMMGYAYSTGDLMSSAGGNGGPGSRLNRRGKSMSTEELAEQMKQIHIHSQQADEQSRALRKAGSEAALTSMDSANSVDLINFGGPDSETSSVGSGTVVCRPAAGNDSTNAPTDDEAVLMHRSASDACIPPNGPGLGGTVGDGTALSSIGGGTTAGLSPIQPSSPAVGLSDWAHFDDSLLTTAAPQPGGPPAGLKPALEKSSVQQSSSQSVAVDPLASLLTTDSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTF7</accession>
    <name>A0ABQ5RTF7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="432" mass="43875" checksum="43C3698CE2E317B5" modified="2025-10-08" version="1">MALALTMQAIGFRTCGNGPLDARHVCVRTTTFGAIRCAGGHARAATVRVPAVGAAAALTAADDEYLEKRFKAEFNMMYSRLGGVVSQAVAAAVLGARGKADICIASHEINVAEEATASQRHYGVPVASLTWKSTPAGMASAFLRCLDAQLQAKKFMSGGDEAGDVLGSFPSALLFYYPGEGLQKVVEHFAIAASEVRGAAAPAAAAVVRSLAGAVARMASQPNAEAAQGKAAAVMTCLDGMAEALEGAAKGATSAMGPGAPSAAAAASLSATDLESAAMVVHLEAGQLLREMAAVLDCIAGGSPDLPACHTEGGRTLSPSTPAHPLLSALETRLFADSTWRNDSAAEIAAGLVDFLAALLAPSRVGNTSAAASAVARGSACAALHSLAQAQAGLAVVMQKHRVEPAVVSRYEGLIAGLDLAGYERLRYVCVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTS1</accession>
    <name>A0ABQ5RTS1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-class carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04645">
        <property type="entry name" value="LbH_gamma_CA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047324">
        <property type="entry name" value="LbH_gamma_CA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050484">
        <property type="entry name" value="Transf_Hexapept/Carb_Anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061">
        <property type="entry name" value="DYNACTIN SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061:SF29">
        <property type="entry name" value="GAMMA CARBONIC ANHYDRASE-LIKE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="280" mass="31405" checksum="43485271EADD3537" modified="2025-10-08" version="1">MLKHAANLLPVATAQLCERAEVQLLSVRSINFFNAPNGPSVKQVNIEDEWYNRQRNIFQLLDKQPYYPVDVFVAPNAVVCGDVDIYGGASVFFGAVLRGDLNKIRLGNRSTVLDRAVIHAARAVPTGLNAATLIGDKVTVEPYAVLRSCRVESKSIIGARSILCEGTVVESESIVAPNSVVPPARRIPAGELWGGNPAKFIRKLTAHERDRVLDDVANHYHNLAAMFRREQFDNGTAWRDVEAWRKKLVDHGEYQWINFREQKYMMRLQHEAEALEKLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTV4</accession>
    <name>A0ABQ5RTV4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="899"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="958"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="496"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="589"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="652"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="731"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="763"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="809"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="823"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="910"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="959"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="971"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="998"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1036"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1057"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1074"/>
            <end position="1106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1141" mass="119465" checksum="01DED16C60F70AD2" modified="2025-10-08" version="1">MSVAFVARVFGPSLPQAGIFRAPAIRPFIVNARRTRKAIEEPIEDALTPAADIGPASSKRIRKPRAAEVTEEELTTAPETETSDPASRSRRRTTVDEDETTNTSEELADAKPKRTPRKKRSEAVVEPDGTTTESNKAIAALVDGSESAADSVEPKRSSRKKTSTVAIDDVGEGGDDGGAEATPKPRAKRSTKKPKEQVFPDVVSTRGRRRAAAESDDDAVPPSVVVAEEEVPLPTASTVATKRRAATRRGGSARQTAGSSTLSDLSQIVIQRSDAGEVVAINRAALGMGRKMWYAVGVTGGMERKWAEQLRQKLANNDSTMLDPETGEMVPISELIVPWVPVRRDWVMAKKTGNVMQRDVVHKEGWVYVNAPLDERVVRIVEECYGFQQWRGEQDHFLEDVGSVKLITPLSEEAVAEIRAWEAASVEEQMDPATKEALSRQMFGEAGGDVLGRSERLEDREGETELDENGRVVYRNPNFTGGRSNPSNWYTGANPAAAGSGTDAADATGEAEMVSQDSRGNELAYAPEPEPKNRDRRQEGQRESRGLGRDRPQGQGQGRGYGQRQGSWQDSGVGARVREGGPGRATSSPPWKQQQQPQFPERQTFPARAGVRPSAAPVTPRPSPPPATSRAPKADLVLKFDDEVDEAPVPTPARGSAAAAPSAAKAPAVDSPLEDVRQPWVLRSDDLDDYDDPDEEGDAVGRIEGPSGGLDMDGWFGSRGVADGGGTATSRAAGQRPATAAGARSGTTRDRGKSNFGKYPALDENDVDPSGEEFDRLDGAYLGQDEFGTFSKDDFADFDFDLMDGEGGLGAGAARRGVGGAASGASSRRQQQQPGGRARSGGSRSGAAAPLDDDLYGGLSFDYMNFDEENDGSASTSRGGGGGYMGDFGGLGDNDMWDDFGSGGRRGRGAARGGSYGAVAGGKLSKGSGLDADDDLFGGGDDDWGYLNGDDAGGFGDFGDADPFDLDADPWGGGGGFGRQSRGGGGGRTGGYQSSSRSGGGSGGGYMSGAGPNYDGDLPEPVAAPAPNADRLNQGQGQGYRNGGGGGGAWKQGGGRRNSWQQGGSGYSNRGYQSGNGGYQSGGGGRGGGRGDGGGRRGYGGAGGYGQQQNRRNGPGGGREGGWQERRGGEQGGAQGAPATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV65</accession>
    <name>A0ABQ5RV65_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003897</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="697"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1018"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="123"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="293"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="458"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="518"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="760"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="831"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="708"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1054" mass="110164" checksum="7E8B1FF3783E769A" modified="2025-10-08" version="1">MSSILRSTRPAGPLAGGKGVGRRAAKLRCSAHIKPTAEGYWTSLVRKGLTAGLAGVLLGSSQAPLGADASATLTELQNAKAEELARIVRERTGRPLPKVEASPSAEAEREEALRLAAERSAAAGSSEALRDQLRNLNEQLKREQMERERLVQRAEELQQRALAAANVQAAAAPSVAAGPSAEELAQRKQLAELQQQLAEAQRAAAASAAQQAALQQRLTVAQEETAAAEAAAAREVAAAREAAAAAAAGATADQGTANEEDSPLNFLGALGIVVGGGLAGYVAVLNQNKESVEESLNSAIVAERRQVSELKDKARDAAAALERERQLVSRLQGEMKQASQDSAKMLELERVEKDAAKRAARLAEESLAVEKRLVEAMRREAAAAAELIDAEKAAKFAAEAESRAIADSLATTERLLESEREVARKVGKEAQEAISRLRTSQAEAGRAVVANSYLSASLDEEASRRATAEELASELQTQITMANTKIAQQEALVSKVGVDALSMKEAMRVLKEQALNISVKAQTDAAAAREEREHVEAELAAVNRQLADERDALNQARSEQSRLKAALVESQARAAGLESELSETRQANTALQVQIRSLQSSVSTLEKQLEEQRAANVAVGTEAASLRSALSEVRSSLGATEMALAAERAGAAANAAALEALRADYRSAMASLEASQADVSSLRREREELQRQLEAVQSELGAAQTGKQDAERRAHDTADELARARQRGAELEVAGGNAAAAARQVQEAYNRDVGAARSRISGLEGELAAQRSAMVELERQLSVRLSQVTAQLQQADEAATSAKVERDNALVAAAQQLSSVRAEAAAAQQTAAKTMAELNEERSARQAAEANLAQLKSQLDALVNRSDDYRREAEYAVSQLREQYEALKAKAAAKRMASNRKGPAAAANGSSAAYSNGNAAAYSNGSATSDPTVEAVATVTAVAAAAAVAAQAAATAAATRTPLETAVASETVTAEPAEPAEANAEDVKAEVPGHLPLHESTITAAAAVAAEAEVEAATTVAEPTKPEPMLASAGATGGIGFGGNVSKKGKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVX6</accession>
    <name>A0ABQ5RVX6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3598 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_003774</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="37677" checksum="950B4837B21EAC92" modified="2025-10-08" version="1">MWSLQLRQHRPASACRSTVQHVAKSLTFSRPRFCVRQHSATKQAQAPTEREASSYGALWDAFSNKVQGEWEGVTVTFNAEGSAQPLPDRFVSGAYREWGVELYDWQSQSSCLVTQGPSNGPSLRNIFKRLMPTVGCEADAIAYVEEAEDVWKADGGSLVKPVTPAGGYVAAPAKLAGKTKVEVCLAAPASQTRLRATLTLMQHWESKQWQVTFVDLSRETYDGPFNGGVELSGCAGGGKPFAKESVTLPEQLIGGWTAMDVYRYRTSSEETGGLELQAGTEQGSTVSVASIEGSTTILLPLGAWVSCAAAESEVKIVVGALRGESERGIAVCSFVEGCLAEVVLATEVKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW01</accession>
    <name>A0ABQ5RW01_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="27469" checksum="46D026DB437AD12A" modified="2025-10-08" version="1">MNMDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTDKTPGHSIADATRAYVLDAKKYLQAVHGGDNDGIRCLFVGNTLEGSAKDWYRNWTNARGNYTFDELVAALLARFATEVQARDVEARNVLAAGSYHMRRDENVPAYQSRFEALVTPIADLTESERVFWFRRGLTKLLAKWCATDLVGAQIKSYDALVAFAKGTEIRLLAGEEEVKSAPRANAVQVQDPLGEAVALDQDFGATRPTPTAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW54</accession>
    <name>A0ABQ5RW54_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginyltransferase</fullName>
            <ecNumber evidence="2">2.3.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004363</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the R-transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.8" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="6">
        <location>
            <begin position="19"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="N-end rule aminoacyl transferase C-terminal" evidence="7">
        <location>
            <begin position="489"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="123"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="428"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI61855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="765" mass="81551" checksum="4160C7499A9253C8" modified="2025-10-08" version="1">MADPGAELSIVYDSGSGCSSCGYCRGEDSWVSHGMRTRRLTVYAYQDLIDQGWRRSGSYLYKPDLAKSCCKAYTIRLDVQQFSPDKGQRHLLRKWRKYLEGDHSAGTEEDGYNALDSIGLASSHGQNTDTNVDGDNLATKSSAGHMLRPQGDPEERRQRGTQAAHPASASADAMAGKPEVDPPGEVNEQVLGELLAAALAAAVAAGDLPAAMGSALHTPPGVRRCTAKQSSSLPADTRFTSAAAFPLASGAVRKATAVGAAHGTGAAAVRNSDIEDGTASVSGQTQSLKPLKPLRKAMPPMEGALSAQEVAQLLAKNLNGLVSRGWTPASGSWHGLVKPEAYALNGHVNIRLPQAQPTDGSRSAAVAMTAAPAGSKAAAEVECPGRGRKGPNAAGVSSSYGIAGCNTGTGCGGGTDRGANLRSNLWIRTLGDPPSEGDLAGSEAVRTPRRGAATPVAALSAPRPMDSALLVMPSGRLQIRMVPSKFVQEEFELYKRYQMAQHGDKPSHLNSESYTRFLVQSPLAYVDRAQDPSAPECGYGSFHQQYWLDGKLIAVGVVDVLPRCLSSVYFFWDTSLASLALGKFSALQEILWVQRNLTTSPSLHYYYMGFYIHTCPKMRYKAEYRPSDLLCPMRKVWVRITPQLLAAIEQRPFLELSAEEGAVCQPNLAAPSSKVTEAASSVLMDVGSANAGAGTLARNDDESEGAGVSVFDTLVLLPEDGGVLPFKVLRNYDIYSRSDLNLIEQEISSWRAAVGWAAPSLLYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW91</accession>
    <name>A0ABQ5RW91_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA replication licensing factor MCM5</fullName>
            <ecNumber evidence="13">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_003864</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="13"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008048">
        <property type="entry name" value="MCM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054125">
        <property type="entry name" value="MCM5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF42">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21933">
        <property type="entry name" value="MCM5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01661">
        <property type="entry name" value="MCMPROTEIN5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="335"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI61468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="754" mass="82827" checksum="95BE5E343DBD8E2C" modified="2025-10-08" version="1">MSGFDGGRVYYSYQGQEAPREPHEHGTGLAEKELIDHFKKFIQTYQIGTTKDVSERRLYADDLYEHRTQLHVDLKDVRAASHRLADELEERPAEVLPMFEEAAYQVLQDMVAADEEGKPVEVQDVQVLLYSSIPLAQSQTMSIRDLESSRVSKLVLLTGIITAASKPRHKATYLTVQCKTCRGTKRVSCKPGLGGAFMPSYCDMADRRAPGAAGSEGCGQNPFVVLPEGSSFVDQQTLKPQEKPEDVPTGELPRTVMLVADRLCCNIVTPGTRVTITGIYSTYKGKAMDKGATSLQQPYIRVVSIMQEAGDAHSRFKFSKEEIQAFEQFAKQDGLHDEIFARIAPNIYGSPDIKKAIACLLFGGSRKSLPDGTNRRGDINVLLIGDPSTAKSQFLKFVSRVAPIAVYTSGKGSSAAGLTATVVQDANSREFYLEGGAMVLADNGVVCIDEFDKMRPEDRVAIHEAMEQQTISIAKAGITTMLKSRTSVLAAANPPSGRYDDLKTAQENIDLQSTILSRFDLIFIVKDVREHDIAIARQVLDNHRLGGAIKARRQGAGAGNSGGASDAGVGGQEAQDVEFLKRYIHYCRSQCSPRVNEDAAKRLAAFYVEIRNEARAQANATDSDSPPVPITVRQLEAVVRIAESLAKMSLQPVATLEHVNIAIELFTKSTMDAVKSGLTQTELGGEQQLGHVRRLEERIKRRLHIGAYMTTRRLLDEMVALGEPESLVLRVLLTMAAAGDVNLTRERTMVHRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWF5</accession>
    <name>A0ABQ5RWF5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_004203</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel family. Plant (TC 1.A.1.4) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045319">
        <property type="entry name" value="KAT/AKT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45743">
        <property type="entry name" value="POTASSIUM CHANNEL AKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45743:SF2">
        <property type="entry name" value="POTASSIUM CHANNEL AKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="402"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="13">
        <location>
            <begin position="505"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="13">
        <location>
            <begin position="640"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="826"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="925"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="1123"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1212"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1502"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1581"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1581"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1597"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1638"/>
            <end position="1647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI61751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1655" mass="175829" checksum="0EACE7E24490AC84" modified="2025-10-08" version="1">MTHPTPSAASFGGGIGNAKSVKCNAVVPVDACDGQLDAAEGGASPFGLGSPLQGERLGRKSLRSTTIENEPTTDFCSTDPSLSTTKSVIADTSVSYGRQQNRLTAQLAAKQIRRPSQVLESLPMEEAAAAAAEGNVGPWGLMKKDLQIAPWNVRLKLRLQKLSHALLVRPSNPRLQTWFYVCVLMALLSGWEVPFHFAFVEPGGLLKPYYDWVAIVEYVSSAVFFSDFLLKFNIVYIDPDSGALVGSHRKIAWRYLNSWQFYMDFIGWFPLDWITIDTAAARGASTRTLVWLAWIKMLTLVRMYRVFELFAGLDYRMVLGQGALMLTRNYTYVFYITHWAACVLYHIAAHQTFSAASWVGRNADRFMGRPVYEKYLLSLYFSVSAFTGLGDGALFASTVPEAAFMILYLLFNLFLGAYILGTVTMLVVKGDERSKAFRERMSHLNDFSKTNELPERLQTAMQDHLEVTFNTEQIDDEHVLSIYPTTIRRKVLRHLYLQPVRNCYLFKGCKQRFLDALLTAARVELFMPGVQLMVEGDNVTELNIIVSGDVLVAEAGINLSAAFSQFASTDTSNRSARRWSTGGGDGSVVRGSFPGVRRSASVLGGLGSILGRTSSTGQNSIGRNSSCNGSIKDFLIDGANGFMEMATGKKVMTRVSGDALAEVSFFTDVPSNETIMSQSVVRVLSIPKVAWEELMGQFPQQARLVLTNLQQRTENALSHELQEATYVTQLTEEALREALQHGKGRKHKAMFKQGASPPVDLAEILPPNTIDFLHRLDDARAIVRAHVRKVDQLRTFEFLGSASDGDIEVLRTMLNQGMNPNSADYDGRTGLMLAASGGHEAVVRLLLDSGAKSDQLDAFGNSAMCEAVKNAHDSVIDVLLGYGATLAMDPMAVASSMCTAVYEGDLIKLRRLLRSGAPPDACDYDKRSALHIAGAEGNLAAVKLLIEEGGADPNFQDRWGNTALDEARRVGATPVVAYLESLLGANQLVGSGVRYRQQVAADFLSACGTGDVGRVRHITHNSHPGCIFTGLILAASKGFKDVAELLLAWVPSDILAWEGHMAMVGAARMGHSAVVAALRSNGVKLPDPRDAVLFCHMRSAVLNVDVRAVAALLAAGVDVRGPESTALLHLAVSTGSLELVRRLVEEGGMRPSAEDSGGKSAAAVADAAKLASIADYLRWATATVGAASDVAASGLVAAAAVRFGPLRDLSQRDTHEDLTHGVTAPETFTETDEDEPPILLARMASASSAGSSRRLTALGSMSRQVSARRSNVLWKLSSMARMAANSQPDEGDSSQLDSISASGGVHSMTAGHTRRARRASLTNFLTGHRVDGITVNPDVEVDLAAILGMPSFSGPSMTQKAGKKQSSSTGYGVSVSSNASGAAAGAVAHRRSSASSHGISTLVMGSTDGAELTGLTGLTGLSTEVAGLREAAGKVLVPAAVKQGSFLVGVRPVSRQPGLPPLSASMRYRREGMAELRGAPAGLPAPESSAVVTAVPARAASSRPPSAAAAGGGGSGPGAASFAPSMPLEGGTAEGGAQGLSPSLSILETPPDDALEHFLKVAPSAFSASKMVLVPPPLPLPLPLPPPPPQQNPGYSAAGGGGGGGDAVISPDKAALPEGQVADNDAPNRRGALANGDWSHRTLEKRLTALGPPGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWK4</accession>
    <name>A0ABQ5RWK4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="377" mass="39569" checksum="77D734E3DA447BA8" modified="2025-10-08" version="1" fragment="single">MSKTRPARAFITHVLTFCLGVILGYSIIFHLYSAHRWQSLSSDQVAASAAAVSASVMAVSASAADTAASTASARAAATLHASAQILAKMQLPELHGQSSSTTTASAGVAARPAHTVISPALHDFVYDIAMSSGHRPLFGATFGSCLVGLMASGAMLHARALHLLLSDAKLLNFSLVHNLAHMGTNVRVSLYDMAGASTSMRARNTFYQLLRVPDLMPGSPSGQTRSVAAESAVALAGSDLPTVLLEDDVWLVDDFPRKLHRIASLAAERAVGSSFVIKIYIHSGRYGRPKKIRALHAKACNVSATRFPIFHQRGAQAASKSATEGGPGPTVVTAFPLSAIVGSAIGSRAHCRGVGHDCSRFASNDEDSSLKVYEPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWM2</accession>
    <name>A0ABQ5RWM2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="1">
        <location>
            <begin position="26"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="259" mass="29199" checksum="227334CD9852E21A" modified="2025-10-08" version="1">MDAVARSPTVAEVRSALATVNNKYHWKLDSTCQTHLTDTREDLVNYRSVSNGPIVEGIAGTACWIVGYGDVELKTDDGIVVTLKDVAHVPEAGHRLISLGKMVEAGWNFLAKEGHLLPPKQNVVREERWHSPRICSLKQDSPLETIMVDCMWFLRALPLHARESWKTLTVLEQSVEEWHRRYGHLGYENLGRAAKMVDGMSVNVDQILEYARTKGKCDVCIETMATRVSYPKSNDRPRDVMDRVHMDVVEMPMQSLGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXG3</accession>
    <name>A0ABQ5RXG3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF5">
        <property type="entry name" value="TRNA PSEUDOURIDINE(38_39) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="369"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="388"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="495" mass="51510" checksum="AC7500BEB57AA424" modified="2025-10-08" version="1">MALKRSSDGSGMGARNRGSTPGGRGLSTTAGVGPKVTVSYFGPAFAGWAYQPGRRTLQGELETALQPLVDAAGGNKRVVISAAGRTDAGVHAYGMPFSFYSWRPVSYDEIMRVVDGLQPGRLRVMRVEEVPRQFHATFSAAWRRYVYLFPLRSTSPPKHTACQTAGAAAAAANTSTTAVTTSGTATASGGSYPSPHQLAPPPPAAVERLGDAGEVAAAEAPYMPRPPPPLRSSLPSAEGLVPRLGSPAVVIPPESYTLDPDPELMGRMLARLEGRLLDFIACARDTPPGKDGMCVLHLCRCHVMELPHPSPLASGTVATAISAATIPAAATAGPLAEHGVLQQQEAVVASSAATMAAAVRLLPLHEPREAEHPHQHDLKQPQPRSLMPPIASASAATPAEPDLGKQLPAVREPEGVAAGTINPRVLCIELVGNRFLRRMVRVLVSTALREATPGAGRCGGREDALLDCLEAGRTATAMPAPAAGLCFVGAGYATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXK6</accession>
    <name>A0ABQ5RXK6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosducin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004860</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosducin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024253">
        <property type="entry name" value="Phosducin_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21148">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02114">
        <property type="entry name" value="Phosducin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosducin" evidence="3">
        <location>
            <begin position="90"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="306" mass="34101" checksum="71B354FE1DE6187F" modified="2025-10-08" version="1">MEDSAGGCCTSKEEKDAADAARFGLPDPRELHDDPVLAGCCEEDLQNMRRAEALRTTLLAVDRSTVRLKLHQEALHIPQQQQQLDPEMDELDELDMDDDDAVLLDLRLRRLVELQRRAAERQQQSSCGYGRVNSLAPGALLERLERLETPGAAAVTHLAVAGCEAGALLDEHLTCLAHRYPGTYFARVAVKRGEPLAARLKVESLPALVCFRAGALVGRAPVSQFGPSGCLVEEEVTSYLRRLRVLRERPGVHDDSMQDDDQEEEEEWKLKPCDLCGRRYPHEHVRAVYGTCHRRSSDGSGDDEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXN8</accession>
    <name>A0ABQ5RXN8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_005004</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amine and polyamine degradation; spermine degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001613">
        <property type="entry name" value="Flavin_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF418">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00757">
        <property type="entry name" value="AMINEOXDASEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="5">
        <location>
            <begin position="16"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI62385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="458" mass="49308" checksum="F50A29747DEA7D2C" modified="2025-10-08" version="1">MTMESFHSVAIIGAGLSGLYAAHLLKDRFPDVVVLEAQDRVGGRIKQVHGMAPWPIEAGPEFVHGRNSVFVRFAEQQLGVKFGEKEWPDWWYFGEEVGGQGLVNDQDVDDEVDKVHDLFGDCGDEAQPPPGRDVSAAEWMAAKGCTNRQMAVAEACYANDFGCSLRQLGLREMIEENRGWDSGETYLLMDRSMGAVITHLARGANIKTSWVVSSIAYGGGDQPGASVRVCSADGRVVRCGAVLVTVPVTILQQGAITFSPPLSAAKTAALSRVRMGNVIKVVLSFSRRFWKEDMYDVVCPGAFAPEFWMLNYPPTNPGAGTPYCIVGFIAGERADEVSAMGPDAAQRRFLEQLDEIFGAPTDPRPASSSVVKGHVVDWSKEPYVRGAYSYPSLGAELGDRAALASPVLGRVFFAGEATNETINPCIQGAMQTAERAAAQIRAAVDVAAVATKPALSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXQ1</accession>
    <name>A0ABQ5RXQ1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004966</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11296">
        <property type="entry name" value="O-FucT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045130">
        <property type="entry name" value="OFUT2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13398">
        <property type="entry name" value="GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13398:SF0">
        <property type="entry name" value="GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="836" mass="92597" checksum="7C0CF6C4FE1A4D59" modified="2025-10-08" version="1" fragment="single">MFDLSRIWRSRRRLFPNGRLVLACIVLIGASIWIYPSIGPISKNNYKQEEAAALSGAKSVVHLKTKTRGVLDIPRNDNTKFLLVQCHRGQFSNRLECLKLGILLARVLQRVLVLPMFTDVVPPVNVSKYVSLECLRRNSLAVTFEEFREQLCHGVGLEASQPVPEDAAVLGVSNTTGSYFQRGFFCEDNAVLQLETSFSLEDYRGYNCWNRREDLTNARDLGIHVSGCTHVPPSPVTPPQTSSYSLADIRRHFGSRPEKVVLFLDLFGVDLLSSTADLADTISEPGQMCSWMPTEAAAAAADTVMQILIPQPLVMASGAIDSNVGADGTAAISTRDIGVGLSVVLDPTTHRALSMGRSELTREPPPVRLRGARRYAAVHLRRSDWFYYCGGYSHCFYSIMEAAAWLNETIAAQGLDTLYVSTNADNREKMMLRRAVGARVLYWEDALTQLRSWQEEGTLAAADAAPGLQAADDGVDVSASWEESAPPEWLISLPLNDDLMVQMVEKAICMQASFFVSSIGSTFSDQIRAFRNGGLEDEEWGVIESKFPSMVTQGSAESAITPNTGAADSADTVASGKVVPLREGATYLCDTRPPRYPDERRLPDYHNPKIFLEQMLPLLTPLRDRALRIWYGITKRKHKESTASAAPPPEWPHWQRPTLSQVLEAPARILEWLTLVAEDGHAMAAANRFVDMYTGGALVALVVEGTLESPPAALVDCVVAAAAEAGAMSLHVTMRNVPDYQQVLLMQELEGRTVLRQVTRMGGPDQERGNTFTLMWRGDGLSRHAEPALLEELPTFLERVDNYILAKARVLLGDRRGVLFRQVENTRRVFRFTDPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXV2</accession>
    <name>A0ABQ5RXV2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005050</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052201">
        <property type="entry name" value="LRR-containing_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24111:SF0">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24111">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="602"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="762"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="217"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1151" mass="118840" checksum="8BF304A916207C0C" modified="2025-10-08" version="1">MCFKPKDVPSKKVVLVELERAKVILEQENSALKVKLKDLQASTVRAAKERDGLICQRAELEAQLQQRASHASHLLTHITCLRTNLAPKLQELLHQHLVGTAQAVEDKRPNADVATATKVNVHVAPHEDIDDVKLCDTQESAPGDNAGRTRKVCGPALTQIGHMGQPGLPPPESADSVNVDRTGAPLSVQQQLDSELEALTRTHQGIIDQATQLCDYLRQADSDKAELRADVAGVRRRLEQAEANAADQQLVLQLRCAALEQENEQLTGMNQELQERLEAAQGELSSAKKQWSSDGAQLAKQTAELQATSVRLSEVEAELRVLQSQLVAATVQAEDANATVIELQQEIAVRASSLNATGSTLATRETELAVVTAQLQELRTQLLEQQLAREALAAELTQAKESGKLAADALNDAHSQLTDAQQEWAAAQRDLAATQRDLAAARRDLELQSQALLDSNMHLQESRTALAATSERLSRALDDVAANEKRVTEANALYLSTKQSVISTAKQLASMAKDHSANLAKLHALEAEATSLRDQLAAARSAEATLRAQVDSNSTAARESREAAEVAGEEVRRLRAAYDTASADLRRKIMLLTAIARMADKGPAGAGGGRSRDGQAADPADGYGAVTSEQRLRLKICESPLPLLGALTAGIMDSSRLRHLALDMDTSREVTWEKAGLRICCLAAAIGLNNSLQTLQLGGWTWGELGNGTALPFLALGGGGGGGVAGGMLRSVQLATNLFDLEAAAMLKDLLAAGLVELAAAPDGPEDHGGQRTSNGDSGGGGGGGGGQGVLYLYSGNERLDGWYREMLVLAGRRPQGRISSSGGGGGSAGAGSNGNILATIGTPMPSGAGPVSRSSGPSSAGSARLTAPFTNGISATSIVLSGASGIPDGDVSVSLSAGTAATATSNGAVATAAAVVTVAAATITNTGAATATAAVDCDLSSEALESHHMVLVMTVLLTCPGMKRLRLDGNKLGDGGAALLALGLSTNTGLRELYLSRNGIRAAGARSLARCLEGANSTLLRLDLSGQRLEGIGAAGAEAMAQALRTNRTLEDLNLSSCGLSGSAAACFAGAIRVAGAVRSQSPSSMGGALPLSSSSLSLTAAGGGVSPRGGGSGGGCLRRLILSGNGIDAATVKALMGAAAERPGLALAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYW2</accession>
    <name>A0ABQ5RYW2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004823</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12416">
        <property type="entry name" value="RRM4_RBM28_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051945">
        <property type="entry name" value="RRM_MRD1_RNA_proc_ribogen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="148"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="261"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="401"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="283"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="310"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="451"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="531"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="541" mass="58843" checksum="03F16BB891EDB192" modified="2025-10-08" version="1" fragment="single">NFSIVRWQARLEVFGAVKACRLVMDKASGKPKGTAFVEFREDGSADKAAAACARGRRNEGPGITLSGRQLEVDLAVTGEDARSLATQRLHSKTASKDRRNLYLAKEGHITEGSPAWHGMSAHDQAKRKRAAEEKNTKMKSPNFVISRTRLSVRNIPPGWTEAKLRKMFVEAVRERATKESPRVVQAKILREQDRYDASGARKSKGLGFVEFESHDHALAALRQLNNNPGTPWGGERRPIVEFAIDNVKVLKKIEHFKEKAKMRQQQLQQQQGREDGTVADGDSGARERLAADAEGMDKPRKGGKRERVGGRTAEGGVAAAAAGAGRGQQHVRAEKASAAAITKKDAGKEANGNTDGNTHGAATLSKRQRKRQRQKERVDRKKALLKEGGVKALVAAVEEGRMTKKAGGGVNGDMKEVHDAKGVGVPRGSAHGRPRDKAATKQQQQQHRKRRAEEEGLDRIAREGLVAMAATRGSAPHPKRRRRGIVEEGEEGGSDRLDKLSAQYRAKLSGERPGKRSAPKPSYAKNAKAYGGKGQGKKSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZF4</accession>
    <name>A0ABQ5RZF4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17731">
        <property type="entry name" value="BRCT_TopBP1_rpt2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059215">
        <property type="entry name" value="BRCT2_TopBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47776">
        <property type="entry name" value="F5A8.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47776:SF2">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE BRCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="20"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="501"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="673"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="686"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1112" mass="118684" checksum="304D47FF7D792D98" modified="2025-10-08" version="1">MIRMYRGAAKVSHKENATRDRQRAFARVVVTGTGFSADEREVLKEWVLKLGLQYSGDLVCRCSTHLVCKDLFRALYGPKYVRALQWGVHIVSFRWIYDSLQAGHLLPTAGYKADEPGPAHVALQQAVSLRVRIAALQDTSRIPLGELCTNSQEKALHDGNAVVLIEHANNPKKRPAGCGQEADFTFDLTSRSSGQDCSPRLCHAVSTVQPTPIAVEDSSGCQPFAAVGKELKAAAEGRPQQTDANAGQREADHTGHLNLSSPSCKPLELPIILPTPSTFEVSFSELNAEVTISVPDDDASTQREGLDITPISKEICNGANVTSYSAGRLATPTDFTAPCSVDPDNDFGSTAVTDNMCPQEAVAGSRGLHIQAPAHTPKAEAAAAHGSVKLPGAPPQLDAVQPPPQASVLYSGFDVPLAAIATVTKGRRLVRTDRWSVGMNGNTAGNDAKAELPSCAHLEDDLEDDLEDSPYHATAEPALAHAFVRAADCKPAAQSCLPAAHQRPPLTISTPHDKNPSSAIESKGSGSTRFLHKPVPASAPNPKAFGDCGSRAQPRNRSENQQKQPAPSLQHDGQLGPRLNQRGSGSGSSVPIKAEPGCGDGETGPLGPAITQGPGTADGVNAGGRGVAVTHPSARRRAAVLGTSSLLSEPEEIAPWPLGRKGTEVPAVAPRRTCSDLNTLSDGYDSASSSRGTSSGLEDLNVGPSPSPDTSARPGSRAGPHQHQLAWEDSTQADEDQTGSISRKTVIGKTAIVNPCQDSIKTRVVSSYVKGFRHESTSEGEESPIDATGSALGPRRFGLGQVRNAAAIKPLPRAIGAKGDPQCRSGNAETAATIDAYVSEDQKCDGDIVSQAEAGSQLTVSVSQSLSLQLEDGSDVNKWPGEPLLDTKGIAEYLRMNALMAEEYLEKECPSAAGGLLSDSHIRVVKPMRRPRASTLALRHPCVSLKTVQFAEQILVEPLSLLLNCRDKSKRARCPLIALTNDDGAPEASMRREFFLGEPCNIYRLPSGDWWLEYYRYFTFDDVTVLAAAAGRPAKLPIDYDNRRELMRDTEPSHCRVSQVRGNMTIVRTKKVPPCERGKPAGSERPAFCRFFYDKGTGTLVTVPQAADLIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZJ4</accession>
    <name>A0ABQ5RZJ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005763</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="393" mass="42513" checksum="A32220DD1BDBF4E4" modified="2025-10-08" version="1">MILDYTACGFAPSLDVDTVTRLYMGPNRDGNGGVAEKFNECSYGKFNISYFQAAVVKPACSTAVTSGCSWWAISSAADAAAKAQLGLPFFSNFTYYTYVVPPGLQSVCPWAGLALLPGKQTWLQSTGYGVLRWATIMHDTIHNFGLWHSWKNGLEFEDYSTAMGRGDSCPNAAELSRLGWATAAAGGDEINTGVLTAPGTFVTWTLPATYITGDNSYLRVIPNWMPTYGNNTLEKNLYIALRVPKAGDAGMSSTYSNRVHIHEVNATMDNGFPPLVPFVDRKIQIINMIPQNTWQVISGYNLVVVTGPLTSSDTIQVSLCRFEASDSSQCSPPSPPPPPPPPMPPRAPIPFPKPLPPSPPSPKPPPSRKPPSRKPPSPKPPPPKPPPRKIIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZR3</accession>
    <name>A0ABQ5RZR3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="573" mass="64258" checksum="6DDAE5E60796256D" modified="2025-10-08" version="1">MCHTFQNLSISLISRTCAVSLRLPGSVLIASSSTLSRRACIIPLHPGLGRWLSYGTSRDLLEDDNLLSSPAMKLCATYVTVIILPCCFNMFTYAHISHVFRAWLGPQHTLFVDGSDCVTEGRHVKGFLDEEMRKGLGDFLDYWTERDHKRGQLLAGVLSTARRLINADNNNNTISGELLDLSKQLGRLPINFVNWPLVRRLVVASLALRQDRHGPFAQEIYAKGLLLQLFAQHKGSARRKAMIEFAHVSKSAGTSFCNLAVDNGCQSDTKRNCLVDAFGDDPRYFDQSYHEVLRGNCNTSCDNIEKSMRSRVLVSCETRRKWLQSSAQNAYANEYTALGGIKSPALAHPCRNMLTVLQLRHPYARVISHMKFAWLIYVLRCGERDARAVYLKHSNRTDVWSALMPAPTNNYLIRSLLGEVVFNMPPGGISSEHLDIARDLLAKQYDVLLVLEDTDLSKIALQYGLGFQRLKARHDNKTPKGTEESLPKDLEQLYELNKLDLQLYQFGVLMARLDAIVYDAASRLVKSGFTVTPSPSSPPPPHRPGCFPCDMSKPHCRDEEADGDRKCGWIGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZS2</accession>
    <name>A0ABQ5RZS2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transforming acidic coiled-coil-containing protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="79"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="523"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="748"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="852"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="972"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1163"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1292"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="383"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="533"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="761"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="856"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="995"/>
            <end position="1007"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1448" mass="149693" checksum="7BC924B7D4BD00BF" modified="2025-10-08" version="1">MDQDRLEYAEASQGGKRRSSLGALSKSKPVTKLKSMFEAKGPAPKIKESGLLGKLKPISASHDATSFAAALSGSMASLKMSVGGPPQQPPPTPIAPLFSVLPAPVEPDVLPQPSPPRSAEDLQMSLPVYQESLPDGGDVDARKSPGSAGFLKRMSSSLKKALTPKSSTGKQKADGFSPSYAGSDASNLSPRPSAQLLMTPGSQPSSTSMDLNSRSDQTQSAPTRSPSALMGYEVHVARDYPEWTRPCEGGVDSTFRPTSSSSRPPRSPSMAHPPSGSVPDGVSAQHEASPPIASRDVSPRPLPTSSPDQSQTFKCIAQDAGAPMGENENFVNADSKGPAVPPVAQDVVPEDSFAASLTASPTVPDCSDGSHGSPPMREVNMGAATSSSPQLPSSNPRTDACDIVADLQADELVFPSLLSTDQYGPVIHIVEAVASHVSDGLGAQTDESADVDMGACDSGPPIEELQAAVPSSDEMPNAERAAAEIDAPVSLMEVDSAAQQEPVFQQPVPLATRRTFGAAGSLNLDLSGAENNSPGSPDTATPTGAAADNQEDDPMMVSPMPQRWSTVPLVVHVGLGQVMPDMGAPDAAQSTSSLPGDATKISPDTARLGLRAFQLNDIEEPAAIVEARAYGEMAVQEEVTRQGDDWSAETPDALAPLQSISPIIDNMNIGTVTPVTTADGHVDGSAATTTAADVESAVELPTDVSAPDLIASVPSVAAPPPLPVGEDVPVADSLEAIEALWGNDDLLEEPGSATAKGLTPQVASSPVSTSKQAESPFTSQSGRKVGPGVVLQVERESLDLFSLRNSCASRVSDSLMVESLDSFESMGLCAKSRAMGFVPMAPLKEGDEDLAMVGRTNGSDGESGTTSAGPQSPNVTFSVNNGGSPDSQATPGLNAAAATAVVTPSANKPACSSGGRGSTSSAAAGMVKAPGSAAKAATPSVAARPSATPSTALKAMAAANAAGASASKACISKRPLPNPAPQPRQISTPGQQVALPSGPSSGSRLPGFTATPASGTSRTPAPIPGVKPSMPSTSAGSTPGAVPARQGVTPRSGGVFSSGKGQNPISGISRPGLFAHQPAVVGRPGSGLFDTPVIFRAAAVPLADTPFSASDFDLDTVDISAEASKAIALLGGGDASIAGAAAQHATPALAAVRGAVMPGTPTLSVLETHVQLLTNKLAEAEDNINQLQNANAMLRDQLSTLQFKLSMTNDLDMERDAREYLEHELSTLRHQTGGLEADLRAAHAEIKRYEQAAAETLAAQQSKEAEWARKEVELEQRMQQMTSEVEAARSFAQQADARNAEAEARAAAATAKCDEETARGRELQVTLGLQAHQLVQVQRARDDAEAKFQRAMEQLIAAQTSHKKAVVEYEQKLLQSSNLVSQINDVRDKYRQMKQHAANAEQRAQHAEAKAQETANALAKANMEKAELMQMCNELLTQLEATKVKGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S076</accession>
    <name>A0ABQ5S076_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PBP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_006177</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PstS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050962">
        <property type="entry name" value="Phosphate-bind_PstS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42996">
        <property type="entry name" value="PHOSPHATE-BINDING PROTEIN PSTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42996:SF1">
        <property type="entry name" value="PHOSPHATE-BINDING PROTEIN PSTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046339493" description="PBP domain-containing protein" evidence="3">
        <location>
            <begin position="30"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1323"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="domain" description="PBP" evidence="4">
        <location>
            <begin position="39"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="PBP" evidence="4">
        <location>
            <begin position="448"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1402" mass="149216" checksum="AA5A8190D4F857AC" modified="2025-10-08" version="1">MAKHGKAIAGHLAFALAILLLAWAGVARSQNNPSMPLYQVHGSGTSNPALIIWRVMDVMMARTKPKIALSYRSIGSGAGGDEFSNGTSAFGCGDVPISVENFNKIQQQNQLKMMHVPYLVGSVSVFHNIPGIGEGLFLPPCTLAKIFRGAIDRWNDPEIVGNNSHIPDLAHVSEKINVVHRNNGSSSTYALTHYLSRACSSEWNNVTSSDNGTGNKPDQNRLVGVTTNWPVDEPERWTTGTNSQTMVEKIAANPHALGYVESGQGLMAGLKEVQILNARNTRLTSTNSEIASEDFIKSQFILNDLTSAKWTDVNPVYSNASSNQWPMVLGTYFYVMANLTALNETGGLIKMFLTYMLSDEVANLLPDYNFIALPKGIRTDLTTLLNSTMTVAPSPDWRIESATTQTFAGQQDFVFSAKRDSYLMNTVSSLTSDSKEAKNTLKLGDAYQVHGSGSFVSALMIRHAMQILQSRARVPVSMTYRTVGSVEAQAELANYVNMYQSYNHFKVGDMPLDSSIWSTLNSTPGSPIGAVLQLPFAISSLGLFHTKSFGRHSLALNCSLLARLYSGSLKSWNDTELIDLNHATSSNADQSASVDITVFAMAAPSGATYALTGYLAKSCNKIWTLTSGPSVTITWPQNVKTSFQGVNITTPELMQAAISATPNSIGYLPTSLGYSSGLNEVLMDVPAAAATTSQQQQQMIRLASRDANLMDVVPQSNAGIFSNVNVDLANVVKSELYGIPGTWPMPMVQYIYMFRNLSGYGYSGPLLRAFIEFLVPGNLTGEGSKLAIQSGLTPMPESVQKIVRDAVDAVTLKTLTVNWDTEDEGLADGDAGAALYTFSANRQSYDVYELEQLRSALEQVQLQLSYGLLTVVRVACTPDTFGYIRLLRADVQDMADSPIRILDEMGSVSAIVDVLSATLRGASGATSQQTALASSTPLVVHPGPLTDAVWRNVALVAPVLQIPVSIRPVAIIYKGQPGLRLGLCTLAGIFSGNITYWNEKQIQDANPGLSLPRKQIQLLVTTTEGGDVGAVLRYIQSGSKGAGCEQYSNIRLTSSAANTTDIATRLIEGGNYSGLALVAASYLVIDGTTGAGLQAAALPSSKSGTFVTPLQAPSTLSNSSCSSSGCPDLVALEYTKAVDAVIKDLPGVPSDASGSWSAIPAIPLTQTDVYPVVRFDFLIVPADVSSFGSTSAAIQGIIKYAVHSAMGARIQAALAPYFSPLGPKAVKEVSEPAAAQLTVARGAGSWKIVSSSTDKVNDTSSTLVLSRAPSVSDLALTGSILATSSVAESSALSLEVVQDEALKAAKQAVAEKQVNVDKLNLTYSIAVAALVLGLILSLLSLALALYAVALIRGSSTSSQYKYDKQMFHMPISGGGDRGDQIELPVQVPFGGSSAENKVLMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0G9</accession>
    <name>A0ABQ5S0G9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006349</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05382">
        <property type="entry name" value="CAP_GAPR1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034113">
        <property type="entry name" value="SCP_GAPR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046338957" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="279"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="495"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="651" mass="69296" checksum="5BB84F2730E6552F" modified="2025-10-08" version="1" fragment="single">HRECMSLLPVTSEMRLLLLLGVLAPTFALPEVRELQFAVNEHGFQNEGALHQQQQSGSLRTQQRQATEVTSTSLDNIDDDKSSSESTDIGLRVHPDGISHYAVSSGLTRQLLQRRTPPPPSPRPTPPSPRPRPSPPSPQPRPPPPSPPPRPPPPSPPPRPPPPSPPPRPPPPSPPPRPPPPSPPPRPPPPSPPLRPPPSSLPPQLTARPPPPSVVCQDDSTTASYCAGWKAAGYCSDSYSLGSDQCKTINNYWCSKTCGACGVPPAPPAPNDPKIGGCVDADAVLNLHNRYRAAHSSPPLVWNNTLASHAQAWAANLAAGGCDLYHEGYEGEGENLFAFWSSDSFALNCSLATKSWYDEILDYVFTNTPYTDNGGKLIGHFTQVVWAATTQVGCAAVQGSQCYVVSCRYTPPGNMAVDCFYLNNVRKNISVSSPPPSSSVSSPPPSSSVSSPPPSLATPPPPPPTASSPPPPPTASPPPTARPPPPSGIKVGGCIDSDATLDLHNRYRANHSTASLVWNNTLATHAQTWATNLAALGCSLNHEGVAGEGENLYASWTTSSIVLNCTRAVKSWYDEVLKYSFTNTPYTDNAGKTIGHFTQVVWVDTTQVGCGAAQGTTSTGFNCYIVSCRYAPPGNFIGDTSYFTNVRPKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0I5</accession>
    <name>A0ABQ5S0I5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005926</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="124" mass="12989" checksum="AEC2D81E202029CE" modified="2025-10-08" version="1">MFATTVPRRCSSAIVTKTSSVPFCAVVSTRPCSLPALKASFNTAAEFLKAKKDLTDAGVGHEHADAMLRNFLPWSMNVATKKDVDDAVEKLKLWLVAAAAVAVAFGAGGDSLLGKVLAAVVKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0L1</accession>
    <name>A0ABQ5S0L1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_006196</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF38">
        <property type="entry name" value="DYNEINS HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="1611"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2276"/>
            <end position="2423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="54"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3118"/>
            <end position="3180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI63290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="4335" mass="481169" checksum="BECDF1731F8E3265" modified="2025-10-08" version="1">[encoded data, 4335 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0P1</accession>
    <name>A0ABQ5S0P1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="526"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="606"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="730"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="942"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="632"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="681"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="953"/>
            <end position="985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1066" mass="106828" checksum="D3992561FCBDCC82" modified="2025-10-08" version="1">MEQQFQQLTPQNGDCQAVVFFHGRKVTTLPSSPVVISVPSTQQLAGMATGNVATMSNTLPTRPDATPKPSNASTGANLTSIELAALTNSIRGDKLRDTRVWVAGLTMSPQPPGPPPATAPSSSETISFTGPCPVLHNFQNPPSKHKQSIPHRRSDDNRLFKQHLTSTVGTAAGGGETSCSMEPSDCRRGSHFSGGNDGSVIDTASGSVGAVTSPVFGRQTRASSATTTSSLGVLDANACSSSGPWVDVVVTTAAAAAAAAGINIAMTTVGTAATTSAAATTTAASATNAVAMTAAAAGGNAASGGNSTAATAKAHVIPAGTGGSGGSWTSGDNPEEAAPLLSAYVAAMPSAGVAPPNEDIRIASKRQRSTGDSIWSASRQHPHPHPHCQQFPAGSAAALTLATVSSSPPLPETPGCGAGAGNAGGGGGAGGGAGGGSSSATAAAVSSPTLTLPCALPPRRLTQQHINTHLNHYPQHPHGAPHHLQHSHQHLLPQGQGRRQSLESYRLPLVTLMPSGVTGASVTPIATPTATNSPAQSDRGAPSCRVVSTPSLVRDVPRTMLDVSDISDKSTDSSDEDPEECGISLRLPPLQPCVSSHLKVLRASVDGDLGPRPRQPGHSFFTDSQDEFDSWQPQQQQQQQHQQHQQHQQHSHSPHGIGRFLPAALRNQLPPFLQCRSGPAMGTGGGGGGGGGDGDATGLSVSQLRSSSCTSVSSRTLTAAVITTTLGMTSPCGEGHGGGLPSASPLRSSGCGGMLQPFSRDGVGEHLLPVVKSILTPMQIDAPAPVSASACTASTAAAFPATTAAAGGGGGGGCAATLSSSLSTVGSVTTAAAPPSPTVKPHTAWPVMAVTGAVAAAAATTTAMSTQTPICPTALSVLTSPRCPPVSGVATTAAAVTVTATASVAVTVTPPTPAPEPLQALVRSHPSAPSPAPTITMMAAASPYPAVPPRPQPQQTTLSSSSAVQTQPQLQLQLQPQPQPQLQPQDHIAPGDGITPVETAGVSICLSDGAAAAAAAAAAVQVVRRRQPVPPPMPPASRSRRGLLSSVSEEPLTTPGTATVSGDPMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S130</accession>
    <name>A0ABQ5S130_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_005948</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orai family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.140">
        <property type="entry name" value="Calcium release-activated calcium channel protein Orai"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012446">
        <property type="entry name" value="CRAC_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038350">
        <property type="entry name" value="Orai_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07856">
        <property type="entry name" value="Orai-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="311"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="38576" checksum="C1F932BBA8680EF5" modified="2025-10-08" version="1" fragment="single">EDFKVGQRGLFRQWRAYNVERQVLSNCRALWDRHVQKNRRDVDEAVEMLKCLAQLGALIAGFAVSAFYDFQYTASSDNPVLPFFGIATALTVGVELNSCVLCTLLLSSVVRIGKSYITEEEEAEYLWRLRNWTACYMETESSTSSVTAPPPPPAPRRDFQRHWDSRCEGLWRTALALFLSGIPTFFAVMGLAGYLKFLSSPSTVIITAVLMSAAFVYTVVLSADWAKALLVGHSARSTPLDWQAGGWWNSPEPAVSLSTAAPSPFGFHRPPDKDSMWPPSADPQRPYGRTIEELPYPAILGLQPWPGSGPALARGMSVTTDAEPDLTAGATSPRPGAGTVLRPEGATAVATES</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S177</accession>
    <name>A0ABQ5S177_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006671</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043186">
        <property type="entry name" value="Str14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44920:SF2">
        <property type="entry name" value="RHODANESE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44920">
        <property type="entry name" value="RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 14, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="121"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="272" mass="29817" checksum="83E5F34FCBEC610A" modified="2025-10-08" version="1">MASMQCGGRVSSRGSTRKPARSSSVAVNAKPSSSRVGTASGGRIIGRPPKLADPVDFESVGFRFDPANNRWLRDDRFAGKFDQTLATPKSGTPYVVWPAIHTLLVSKGLRSVTPEEAQALQGQGWTIVDVRLATDYEQVHAAGAFSLPLYRYVEGTGFWDNVKKAAMAIGFAMRATERDPEYQSKALSVLKKNQKLILMCSIGGTLDTLVDLRKGVKPAIRDPERAFGRESRSLKAAYELINAGWNANNLFWVEGGLQQWRFRGLPTEGTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1D5</accession>
    <name>A0ABQ5S1D5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="755"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="920"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1108"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1240"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1393"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1458"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1705"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="376"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="831"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="926"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1108"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1131"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1329"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1346"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1410"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1479"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1521"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1531"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1555"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1567"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1585"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1637"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1728"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1758"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1879" mass="190493" checksum="387D19DA3482416D" modified="2025-10-08" version="1" fragment="single">MEALSWRAAREQLLAELQAAADGNSQAGHVEGLRTSQAGANGEASASPATNPRRGAKESASGYAAAAATTATGPSARPRGVVLPLSVAVAQRQYDQLLSQMPAITSGVDSPRRPGSGGNVPEGLRQPASTQRRSATGLTTIPPQQQQQVAPGTGEGSAMGQGGNQPYRHGPRGSGPGAVDNRMGTGGVHFVSTRSRPTRASPMVDAPVAVPPPYGRSGGSTAATAGAEPTLPNGPSELPLLLPQVQQTPQQQHQYRVSGTGGGGGGGGGGASPMPRQKRVSQSNAAGAAGTDGMVRLPSPAMATHGVASGTPSAAISADPLAGGMPPLPRASRKTNGAGNIASSALVGGGNGTAVQSPHHHRRRRPTSPPPPPPMKTTTTITQSQPQPYSGTDAPGGPGTSPGLGAAAASVSRQPPLKARLGSSRVPALPAAAAVIQPAAAPLIAQGVRDQDSLLAATVTPLHLLTAAGAAAATATESVSSNPIPLTAAALALHTTNLHGDDAWRETAAAAIEAAREAASTSGAVPGSLPSAAMIAAVPAVQLAVPSQLPLASPTPALQSPSPIPPANSSPPVGPDKKSSFPPPSLGSVTTVCVQDQGHGTALPGLVVAAAQPATVVTGLTGGREYIPERVRRISDAAGAILVLGRPPSATANIGGASAAGGNGSWTMPYPIGSVRTSETGDGIRIVANRRPSATVRRASQLSDISGSPLNYGSMTQGSIPASASAMPSVVAPEVPGTSPHLLHLHLQQLRQQYGQVYPPSPPHQGSGSIETPPPQQQQQKIPEAPAPAAAPVAAAPSPQPEIVAPLAVLGGPPPPPPPRVAEKLSQLRDQQQGVQEQSQPQQPQQPQQPQQPQPQRRQSTLLGSVHDTPREELTSSGITAPRVSPSPSPSSSSSLLASLPTLPEHDAVSTPVSVAAADVGTLLPDSPPPPPPPGGDTTVHAVAVDCSIVVDKGRTLEAWPTLASYEAELPVGVTSAVANTIGGDFDEGTDISLLCPGTCIEMESLEFPAAGDYVGAIRQPLQLQPGATPVEPLGATAAEDRGDPLYGRPNGSGFHARAPAEPLPPAAKEREAPWYGSARSSASQGAEAAAAAADGADGAVDLALTSRSATANSERTRPQSRPSSPAHIVAEPHQRREDQALVHASGEAGPTSPPTAGLPDKEDEASGTGKPGTTRQSPVVAFTAEVLEEQAVTSLVAEAAAPGLLNALGLDSHEALQAADENRDVNVLPPPPGNIVMAGQKTGPRRATFAADVHPGSGDSCDNGDADDPSGHGRHVYVPSRVPPQSARNQDPTEADTLGDVSAVVSATGAMAEGDMEEEAVRRRAAQRRASSARSTTSRPASAVSRRHLGGTSRRRHVTQSNVDASAVAAYTSGDPDGEQVATLRSLRAGLGRTISSSSRRGARTNDGAVGGGGGGDGGGGGGNSASGGVGTIAQLSRAAAVVGSAQDGRKVIMDVMVVRPPPRRAYHGERRTDSLNDGGGGGGGSDMGSDDSDLEELRRSLHPRSSSMLAGAAARSRPMTASSARSGISTTKSRLSTSRPSTSRPLTSRPSTAHQRPSTAASRRSVSDEYPDPHSHHHRHQHLQQQQQYDGVRPVSAARSVRSTRSFGAPRPSSSKSTRSTRSRRRISDPDSDDLSTISDISDVSDFSDEPSASASALALAPQARHAARLPSARLRSETTFRNHHQYQQQHNQHMHTTRIGRPILSEPGYYPASEGSAPVDSRGQSRSTHSSNSFNRSTTTRKSNSIEGEKSTTGIGPGAGAGGWPGTGVQRGRGDYNRNVANVAASAGGGGAGMGGFGVGVGVADDDDDDGTNDVTNDADADGDLKATALAKLVAADDLADSTGGKADLAATGGADAAARGPTMAMVREAHAQLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S289</accession>
    <name>A0ABQ5S289_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">T-complex protein 1 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_007161</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03335">
        <property type="entry name" value="TCP1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012715">
        <property type="entry name" value="Chap_CCT_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02340">
        <property type="entry name" value="chap_CCT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI64007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="549" mass="59372" checksum="D90CF8D78FDCE8F8" modified="2025-10-08" version="1">MAAAAFGQRLQIAGERQSGQDVRTQNVTAVTAVANIVKSSLGPVGLDKMLVDDIGDVTITNDGATILRLLEVEHPAAKILVELAELQDAEVGDGTTSVVILAAELLKRANELVKNKIHPTNIIAGYRLAMREACKYIEERMATPTEDLGTETLLNAARTSMSSKIVGAEGDFFARMVVDAMNAVKVTDEITGRTRYPVKAVNVLKAHGKSARESLLLNGYALNLGRAAQGMPKRLEGGVKIACLDMNLQKARMMMGVQVLVNDPKELEKIRERESDITKERIQKILDAGANVILTTKGIDDMALKYFVEAGTIACRRVEKDDLRRIAKATGATVVLTLADMDGNETFDPAHLGQAEEVVEERVADDNMIMIKGCKFSKAVTLLLRGANDYMLDEMDRSIHDSLCVVKRVLESGNVVPGGGAVEAALSIYLENYATTLGSREQLAIGEFANALLVLPKTLAVNAAKDATELVAALRAYHYKAQTQPDQATCAQFGLDLVEGKLRNNVEAGVLEPAMSKLKMIQFATEAAITILRIDDLIKLEPQQDAGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2P9</accession>
    <name>A0ABQ5S2P9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box/LRR-repeat protein 15-like leucin rich repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007335</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="3">
        <location>
            <begin position="226"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="513" mass="55604" checksum="DB37DFF8A29EA0B1" modified="2025-10-08" version="1">MAKKRSRDNTQTAEQENGRRKQGESCEAGSRRKDMASIARRRAAHFANFNADEDTSENNVHVGSDQARTLGPWSSAMELVNAREKAQHDRQAKLQGIKGATEEGHLEDEAEWQPRRDPALGPHPQESVRSLFDTCLDVLTTYVDCVESLWGVPDVIKVRLATSVCSHRKMSPEVARLFAVGSPAEMQLPDCTQLDSVVLREIIQQVATERLQRLELGFCGRGFGDEAAALMAAAGPLRALEVLALEGAYRLSDAGLEEALSATPSLTWLAVPQSSRLSGSLLDKLPSLVPGLTHLNLADCRGIDADSLVTVLPRMSALRTLKLDFIPEVDDAVLAAMGSLSELRELSIRCCQTITDAGLTALAATRGPVLEMLRLDECGGNVTDRGVEALASQCRALRVFSARRCTRLGDKALAELLRAGTVQHLSLSGVTGVGPAVAGALASCCQHTLEHLDVSFCRKLPDRCLGPVLDRCYRLRRLVIFGCSQLSPRSLYGHFNIGLVIDGVHTKIGLWTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2Q0</accession>
    <name>A0ABQ5S2Q0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="248" mass="27342" checksum="C7E40CA1E37BE69E" modified="2025-10-08" version="1">MNIHTFCRATLLGQRPCSTAEGFAIVQDLLSNGTSGLANDLQANLYKLSSQLGVLKQLAEKKGVGGAADRQLLAATTPGPPATAPGLALQTHQKQKRKRADNQSNAVESVNPLTGGEEHSRKTGGKKDRKEETGVKKNGKEGEKDKDGKKDKDGKKDKDGVKDKDGKKDKDGKDKDGKKDKDGKKDKDRKKDKDRKKDKDGKKDKDRKKDKDGKKDKERMEGSRKRPAEQSEMGKDTVQEHKRQRVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S351</accession>
    <name>A0ABQ5S351_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37844">
        <property type="entry name" value="SER/THR PROTEIN PHOSPHATASE SUPERFAMILY (AFU_ORTHOLOGUE AFUA_1G14840)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37844:SF2">
        <property type="entry name" value="SER_THR PROTEIN PHOSPHATASE SUPERFAMILY (AFU_ORTHOLOGUE AFUA_1G14840)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="619"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="388"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="620"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="850" mass="88214" checksum="D89146E208A8DBC5" modified="2025-10-08" version="1">MATLTVVSDLHLEARGLSLDEIIDPDLISDVLCLLGDIGDPSSQTYRDFLAACAARFRTVLLLAGNNEYRNHGGSGRTMAQTEDLIRAAAAAHPNVHFLQNTAHVMGNVAFIGTTLWSYLPDTPLPPPSEESGSVSGGITAGSAAVSTRGAADACNSATPAAPLPLQPRERQAQLMSTGMPEASTTNLYLQAPNVGYVNSRISDSIAANTHGDVSLAASRLMSIGFQQQEQQQEQQQRQQQQEVQQTHTRQQAQPIPLVQVTQSQLPPLPLPIPSPRQGRPAAAGGSGGAGGGGGNGPQPMGAVAPMPRFMSHSQPPPDWLTSHAFGIQCSAVAGSGCDGACGGTGSASRPCRPRQLRHCVSLASLRPDEPCPSWRAMQAGCRRRGDGASSSSESDALGSDTDSNQSSYSSDAENNDASPYVIGDDCKELARPRRMEASKTKRQVAAAAADLEHQASAVDTAAAAPAAVAASSPGSDAAIYRTSTNSPARDAPASALSTSEPMNILPVTRLLSQPVPNYVITTSIPAFLSDPGSLDPGALEGTDSTETCKPGALENPEAEGFTATGLSAGCTAGRGAPVFEATLELTLQTASVLVSGSASALVDSGVDTTGVCTSSVLKPRSHSHHHHHHHHRHSSNTTFDELPPPATIREFIGATSLDYRRIFVSPGGPAITPDVTNALFLASLEYVRTAVATARQRGLVPIVLSHHAPSMRGTSRPTFAGHPSTHAYSTDLEPFLATSGIAAWYCGHTHYNFDMKLQGGVRLASNQFGSQARPADGYSRTWQHRLPSSGSTVDALQLGVASAALNGTPLAALDCLRALSWTGSESPPAAVTTPSAFGSAAGAASACAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3A5</accession>
    <name>A0ABQ5S3A5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007571</name>
        <name evidence="3" type="ORF">VaNZ11_007575</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="2 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="159" mass="17873" checksum="A9D3DE8B23068159" modified="2025-10-08" version="1">MMVLATNAACIHQKEEIQAARRRQAKSPQPSKARNSSRNATNSSKAAAKKVTNDGNVRPSTKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRRKKVKSASEDVRCTRARRQRGGSGSKKKQELFEEEVSKDERSEDEQDDATSAREDDDNDDSEIEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3Z3</accession>
    <name>A0ABQ5S3Z3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007780</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03750">
        <property type="entry name" value="proteasome_alpha_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1 2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="4">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="235" mass="25428" checksum="9B6A6ADC1E5F546D" modified="2025-10-08" version="1">MAESQYSFSLTTFSPSGKLVQIEYALNAVAAGATSLGISAADGVVIATEKKLPSTLVDESTVQKIMTITPSIGVVYSGMGPDFRVLVRRARKTAQTYYQTYKEHIPVAQLVRETAAVMQEFTQTGGVRPFGVSLMMAGYDDNGPQLYQIDPSGSYFAWKASAIGKNMTNAKAFLEKRYSDEVGIEDAVHTALLTLKEGFEGQLSGANIEVGVVGPNRSFKVLTEAEVNDYLQEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S468</accession>
    <name>A0ABQ5S468_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="388" mass="43854" checksum="9A7E806086F5F17C" modified="2025-10-08" version="1">MLPTETQRGTYAPGTTKRSTVNMYDSNTAKFEPLPDGPYEAPTITSQSAGHEHFDSSREVKVKDTYGRELPVTSYRDHYPLKEPPQSGVPLELASSGAPFTARSTYDDEFFEKPRMPRPVEPLTYTHRPAPMITRDTTNQDTYRPFEVAVEPVAVNTREATAPPPMVPSIYDTTYRTQYVPKAGEPRVPPGAIPQKDPLPWLNDGTTYRNDYVPKPLAILPPPDYDPYKPVPFDSTTEYRAEYPTKENPPQLPPLTGVLSRDGLQLPLPRRSLGVEFVHRGQSDRYFVLLPRTVDPPCSARQVFTTVHDNQEQACILILYGDDPVASNNTLLGQFDIVNIPPAPKDVPRIEVSFRLNKDLFLTVEAKDLDTERHKRWLQRGEVVILRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S495</accession>
    <name>A0ABQ5S495_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_007721</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="141"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI64449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="221" mass="25819" checksum="ADE2B7CAB6F86D1B" modified="2025-10-08" version="1">MTMLNDDPTTLILKGLEDARPARSLSPKRSPVRSPRRSPRRGPSRSPVRRDVRSRSRSPYRSPSRSRSPPKRARSQERDVRRRSLSRSRSRDRDFRRRSRSRSRDRRRSRSRSRDRRGRSRSPGVRYGMPPPGRTPPRVGTQLFVAGINFICNEQDLERKFSRYGRVKEARVVRNPISGESRGFAFVAMSSEIEAADAIRDMNDREWNGRRLLVQIARNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4C2</accession>
    <name>A0ABQ5S4C2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Succinate dehydrogenase assembly factor 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="entry name" value="Flavinator of succinate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469">
        <property type="entry name" value="PROTEIN EMI5 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="YgfY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="18976" checksum="CB657BB0AB2AD3FF" modified="2025-10-08" version="1">MLRSAAKRFGAVWLQPVPAVCSLPSTSYSLSSSSSSVDANTSEERRKGMVNKLLYRSKQRGFLELDLLIGLWAETHIPQMGLVQLNEMAQVLDEENPDLFKWLTGQLQPPERMSKNAVFEAIRSHVAQQVQDSAPATTRATAGRDWVRGWDDSWREANKPSPEPQPKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4F9</accession>
    <name>A0ABQ5S4F9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047558">
        <property type="entry name" value="TPR_END_plus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="640" mass="65520" checksum="B683878185024385" modified="2025-10-08" version="1" fragment="single">DLVLRMGKDDRQSIGSGRTSRKPETAARNGEQSSRQLADEADSLQQQGERRKGQEAAAKFEAAAAKYQEALDAAVCQRRLYGFSLIEVGANMRTPTGGNVISLEEAIEMRSSLAECHQLLADALVVAASSRPDTELNPQVERQAAATAVQHLTTALRHYACCHPSAVLTSQGPHATADVTPGAGVGDASLPPAFTVAAAAAVAAATAAAALPPEVTVNAGNCAASLGELLEEEAERTATASSPPVAWLPMYDIALQCYRSAASSYKSAITREQDVLTMSNLGDVLVQCGTCLYGVSRALRDADTVLAAVALRSPTAAAAAAAPSEWAAAREAEAQSDFMSAMSSYETACSMCDSSHGDDLPGLLCNWGSALRSLAGCLQDPAARLPLLEQAASRLDQAASFDRADPAPLCSLGDVLMAAGEAAEAIALAAVTSAAGKAPAASAEVLSAMAPETEATAAAAAAAAARHWYGIAVAQLQAALARGYSAALTLRRDEPEALVGCGEAHLALSRLARWDPVAAAAAAAATGSGGGGGGGGNGDGDQNAAGDDAKDIASQHATAAVAAFQAALTRPERLGGWRQRCDVRYNLACALALLGREQEAHQLLVALLGCGAVRPSELARDVDLEPVRHLPWFQALLAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4L4</accession>
    <name>A0ABQ5S4L4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045715421" evidence="2">
        <location>
            <begin position="30"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="172" mass="19650" checksum="C78FBB42243D35AF" modified="2025-10-08" version="1">MFSSHPYLPMHTYLPISLLLALSTSCSEAQQTRDEDKRLAAQMATTQQLLAVVETEAHELRRQLGEQLREAKKARETNVDYFNSDARLALRAEIEIEKEAFRQLRAGNIQAVMQLQAKKKTLQRWKVEADGAVVVGSRPRDTPVGRLADELVDIYGRYQIPEMPKYEFSNLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5R8</accession>
    <name>A0ABQ5S5R8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="329" mass="32430" checksum="6771134102421B89" modified="2025-10-08" version="1">MGGERIRPQCTVQPTGAGPQVPDAAVLGESSEVAPAGAATTMAATLTTTAIATAPTPTTTVTTPIVITTTNVLASAGEDASPPGRLVPQKRQHWTRSEAASASDAAGRHCRTARAVAGGGASVLTQIDNAGALIGAPHKGSVVTQIDNAATAPATLQRPLLAAGSVLRCVSDLELAVSHLRRRDPKLVPLIDAHGLPYSLVASTATFAAAAAPAAATTSTTSKALTAADSYEDPSLGCGKGSGGVRHSSSGGCGSTHVISTGRTLPVAVPPLQPSTAAAAAAVLETASVVETAVAAAAVAAAACFPLWPARWSISSSPQTQHPLSGAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5Y5</accession>
    <name>A0ABQ5S5Y5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008537</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051144">
        <property type="entry name" value="Formin_homology_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45733">
        <property type="entry name" value="FORMIN-J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45733:SF17">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="684"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="364"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="446"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="904" mass="93649" checksum="E0E88028987A465E" modified="2025-10-08" version="1" fragment="single">MYNRLANTHIKMSPGCVCAFLHVRPSATRRALFISILCVIAISIQLAWSDEENSDIAGRQFLESREKRPSWAGSHPHPYAPGKDPTSRAEYNSGNSISDTGISTPLFAGTEGSPTGNSHKEEPQQKAVAVADDSSNVKVDSEEHSQRHSQEAHIAVSQTGLAWDPGATAQPLGQDPDLVQDSDLGQENDRNNIDETKFKLLQIRSTEQDQRGYQESYQQPRLPRRTHPSVRGERISSASASVTTSSTAKTSSLYSWLRSWSWVSFGRFGSGLQSQSDSSKSKSKSESESKSGLSSGSGDGLTVDGDHGDATSASYLSDPELSSFEEDDGNREGANGRLANGFDAQDEVTEASAIAVTNVRLNAGSTSSSSDAAKAGSTSSSSRHSSSSSSSSSGIRSCSTVESSPDSDDSDTPLRSSSNAGGTDEGNVNGGNGAGSGGGGMPDGSATATATATAPAVKPRRRSLVDNGNYYCISSETGSCSPCNYDSVYQLEPCYNDDDHGTNSDVCFKYGSWYSTVYGYTMCSGIPEWLSFVNVTLASGSMVVAGLLEAWEDTGGYMVVTLRLNCPWLMKLDSGTTGQSLDINVKQIGVSGATEGTWTFSTTSTTDPSAPFRSCSSVRFPVRGAAAGASAVSGAACSSYNRIVTLKASLVQKTANLNTFTCDDVSGTDISLSYVKVVGTMYPPPPPSPPSPSPPPLPSPPPSPPPRPSPPLLPPPPPSPPPGPPPSPPLPPSPSPPSPPPPPSPAPSPPLNPSPPPAPPPPPPNPPLPFPPPPSPPLPPSPSPPPPNPPSPPPPPPSPPPPPPPPRPPSPPPPSPPPSPPPPLPPSPPSPSPPPPLPPSPPSPPPPPPPSPSPPLRPPNPFPPPRPPRPPPPSPPPPSPSPPPPPPPPSPPPSPPPSPPPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S634</accession>
    <name>A0ABQ5S634_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import receptor subunit TOM20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_008736</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the translocation pore.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Tom20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010547">
        <property type="entry name" value="TOM20_imprt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32409">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32409:SF3">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06552">
        <property type="entry name" value="TOM20_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="22109" checksum="EBD7C172BAEB3496" modified="2025-10-08" version="1">MSLFGDDPDREQFFEVARQEAQRQYEADNSNVKALVQWGGALLELAHYKQGDESAETIKEAIAKLQKAISLDSDRTDAYWCLGNAHTSLGFLCPNKAEANQSFKEATKAFKHCYEKEPSNETYKKALEMTAKAPEYYDEIQTHIAQSGGGDASGGSGPKKGSVSTSVPEWVWDVGGWVLLAAAVTGALLIAKASAPKVAAAATGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S715</accession>
    <name>A0ABQ5S715_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45134:SF22">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45134">
        <property type="entry name" value="OS08G0543275 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="140" mass="16007" checksum="7D8E9356516F882A" modified="2025-10-08" version="1">MERHQRIAITIQQAVLSDNLRPNPVFMALMCVCMCVCVYVCVYVCVYVCVCVCVCVCMCVCMCVCVYVCVYVCVYVCVYVCVYVCVYVCMYVCVCVCVCVCVCVCVCVCRHSLLHYWYHNLVWSWGRVLQAGRVCVLRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7A8</accession>
    <name>A0ABQ5S7A8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="13700" checksum="860D99754893E121" modified="2025-10-08" version="1">MANPLQQRDVMGLVDKLDQAVAEMLPRPYELVSGARGADAARKFAGAVGELEGFFRQLQELKPVLSGPGQAREVEDLQSEIQEKRLSIISCLHKRIVGLLAPMLRPAGSTITTTFAALSLCPQRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7L5</accession>
    <name>A0ABQ5S7L5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022227">
        <property type="entry name" value="DUF3754"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33645">
        <property type="entry name" value="AMINOPEPTIDASE (DUF3754)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33645:SF11">
        <property type="entry name" value="AMINOPEPTIDASE (DUF3754)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12576">
        <property type="entry name" value="DUF3754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="346"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="573"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="577"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="607"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="617" mass="69171" checksum="A8C2E87C93066BD9" modified="2025-10-08" version="1">MPKEQKENKEKKEETQKDAVSTGCTIICPPDLHIPVEFQEIYSLVRTGMSISERARFEQIAQLVESLYAQEFMALKRSIRHNFLRFSMGAKDQPLISCVGAGLPTVAELDISELKLIADFMQLMAAARFHILTTREWELAKSDKFMFSLPVEVNWSYYDDKMLRAFWASNSERRELRAVLPDMADRVLVFHRGITVAQASGLYTEEKVDLLVEYLVTKPLAKLWNWLRNRKPTDGTAGPTGNLTESRHQARKVVERRTLRLLMPSAWAVIKNFHKKLKLQEPAFKEVVVLYRAANASRQKKHKLPAIQRPVEQRQREILAGRNIHIKCFHDIPMADMDVIFAEKKVYLKMLTIIQMVITVVGGLVAALMVILKGDKVDMNVLWSSISLVAARCGQVYTSAQAERSKTIQDMVNILYDKTDDAQEGVVSMLLEDMADQQLKEAVLTYGLLYSREAELTEAMIDMDCERFLLKNFDLSIDFAVEDALPRLEFWGMVSRITTRLGQTKFAAVHPETAAARLMERWHRSFSSLATPPPTATFPLSNLISDRERAPALNRENLFKAAAGGEGAPVEQLQPMAAAPAAAPAAVPAAGVPPEGKASSGGGPGGLLSTLSMKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S865</accession>
    <name>A0ABQ5S865_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008947</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="520"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="838"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1000"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="842"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1000"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1070" mass="108581" checksum="19A497D5B66F863B" modified="2025-10-08" version="1" fragment="single">GSSTVQSLVLVGCDAGDGVTLAFYVCFPHRLPVQLVEAVRDSCQRLLDRVLLRAITPKVTGELATELGALRRGTPGTFAVVQMPYSRTGSVGGWTCAPFPPVAIVTGTVPAPLDVTGTVIGSSTLGTDLHFGPQFVSTSATITATTTTNTIAVIPQPAAGQATDGGGSSNAISGSSCNPPQPTLNRTVSTAAARGTRLQLDAGGGVASGACGCAGSSGVGSLHGPESTRAIATCAGIGGGGGSLSELLCHTSSTSASRSRSVTTVRPHHLTTAGSQPGGCITGTGGIMGKAAGDRRKTLAGLSALSTVNNMSGVVVLSSRVPILDTSLGGGGGGGADSWMQIVAGNTGPGSVADASQAFLSSSHHVSGSQMPMAVTPPSPADVLRHTASVIVVRDLEAETHGSCQQQLGALVSSVRESITIAATPNPAVGSCLVAANTAPVMSQVLLLGDGADGAGGAAVGGSIVVRQSGPTTPALYLGWEGSGAGSPPFGIGAAGVGVITNAAAAACGLDSEAADVADLRLVCKLGQGGCGSVFLGQMGPGLEVAVKLLELPSCFDIEHFVGQFSIGPGFGPRLRGTGTGTRNNTAGCGISGSGLGGGFGGDTDLGAVLAAEAVRQQVRARRLLLQSATELAMLTSISHPNIVQVYGVYSDVILETRTTSEGQLSYRLRQHVEILLEEQLMETAAAAGTATVDDIRPADYSNVCSAVCMTLCDLGSLETALTERGFPSPPEATARLGSARGSGRGPNAALGAAGTQGKAVDPNSLAFGVNVQKQMRSVCLTLLEVALALRYLHGRNIIHRDLKPGNILLKSRTPTREDPRGFTTKLADFGLALVLDREPIDPPPSTNVTMQPPPPPPPQQQQQQQPGGRLGPDSGSGHRKVVTPPVRYALSDQPCGTVDHMAPEACRGRGSRLTAAVDVYSFGIVMLEVVTGGRRPFGNMPMERIGRLVMAGTRPLFPAWVPTSYRSLAEACWAQDPAARPTAAQLVVAIRQQLHRFTGTAGSSGVGSDSAGPSATPKRVPSTSHVFSTASYNRATTINNDYQPIAANIVANAADAADAQPNAVHVGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8A5</accession>
    <name>A0ABQ5S8A5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_008987</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07840">
        <property type="entry name" value="STKc_CDK9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF546">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="40"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="365"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="390"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="517"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="553"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI65421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="563" mass="61152" checksum="B6BCB9611446137F" modified="2025-10-08" version="1">MAHMNLTSSNTPLTRIEMPSSNSGDDVFGGTRSIHSAYVFSVDKQIGEGTYGQVFMGHDRKTNDKVALKKIRMDTEKEGFPITAIREIKILSTLSHPNVVNLREIVRSEIHKNNNFKGSIYMVFDYAEYDLTGLMESTKYVFTEPQVKCILKQLLKGLAYCHNNGVLHRDLKASNILIDTKGTVKLADFGLARPYNAENDGGFTNRVITLWYRPPELLLGAVKYGGEVDMWSVGCIFAELLTGKPLFPGKDDMDQMDRIFQIMGAPTEQTWPGVTALNLKLYKNVPIDKYPRQHRLRDTLRSKAMGRHISEEAIRLLEKMLCLDPKRRITAADAVMDPYMWADPMPCEPQQLPCRGSGHEFTMKKRRNDQHREQQQVGPAMALPPVPTYGAGGNAHHGYGPGVGPDPKRPRYGGPGPQPYAGGGSHQRPPQQQPYNAGTSSSYGSHTSSQGGYGRQMGGGGGYGMGGGQQQQQYTQRQDGAGRGYSGSGSGMGPPQMGGGHHSHGVGGQSYSTHQGGQIGGGSGPGGYRGGAMGTQQQGGGYGAGSSRGGPQGQSQGWQQQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8V1</accession>
    <name>A0ABQ5S8V1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Selenide, water dikinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_009911</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the selenophosphate synthase 1 family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02195">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00625">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
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        <property type="entry name" value="SelD_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004536">
        <property type="entry name" value="SPS/SelD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002098">
        <property type="entry name" value="PRK00943.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00476">
        <property type="entry name" value="selD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256:SF0">
        <property type="entry name" value="INACTIVE SELENIDE, WATER DIKINASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256">
        <property type="entry name" value="SELENIDE, WATER DIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0711">Selenium</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="10">
        <location>
            <begin position="118"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="11">
        <location>
            <begin position="237"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI66209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="44075" checksum="0B6404A98D5F0C1B" modified="2025-10-08" version="1">MKSIFRLCTSITIKQQKWAFPSLLGSQQPEIHQWQERFLTVSRSSSLSSSGSPSTSSGGASTSDDAAAPEQPHVRLTQLSHGGGCGCKIAPSKLQEILHNVQLDFGSPELLVGASTSDDAAVYRLSPTQALILTTDFFMPIVDDPWDFGRVAAANAISDVYAMGGKPLLALSVLGMPVNKLPAGVIRRIIAGGADVCRQAGIPLAGGHSIDSPEPIFGLVVAGLAHPDHIRANSTARPGDVLLLTKPLGVGAMTTAVKKGLLDEQGYRQVMATMTQLNTVGALLSSDSRVHAMTDVTGFGLLGHLLEVCRGSGLHGRVAAGAVPLMGPARGLAARGVLPGAVQRNWDSYGASVRIGSGVEPWLRDLFVDPQTSGGLLLAVEKGTEEEILRRVQDAGFTSAAVIGSLEAPSAGRTAGNVSVEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8X1</accession>
    <name>A0ABQ5S8X1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like-conjugating enzyme ATG10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Autophagy-related protein 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_009703</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14957">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14957:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0072">Autophagy</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="247"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="278"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI66099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="370" mass="38959" checksum="C4617D68276F141D" modified="2025-10-08" version="1">MLASSNWRQTLQLFQSADNSHPWQLRETPMGPYLAMEGVPRLPIRQANADIIGPAVQPVLLDYHVCYSHSYQVPIMCFQVASIGAVPYGDSITQGTHGEPLSLSSLREEIFPLWPSEREQLQWFVMQQEHPLLPGPCWLSLHPCHTATVLALALGLDVGNVRRGDVAVDGGAADGSATSAVDCGGDGDGGGGANGVSAHAGAFSSAVATLSAPAEPHERHHRGATAGHCLVMKNYPVRANGTVKLLPVPSVTGQPQQAAGRGGSANEDVPDLDQLLDSGGAYSSAGSRSVNPGRENAAAMEPIESMAGGLEAVQATIRGVAVGPPGMQWQGGDVPFCESDPRSLQHYICVWFSLVGPHVGLEVPMDMMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S972</accession>
    <name>A0ABQ5S972_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_010310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013937">
        <property type="entry name" value="Sorting_nexin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051837">
        <property type="entry name" value="SortingNexin/PXDomain-PKLike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999">
        <property type="entry name" value="PX SERINE/THREONINE KINASE PXK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999:SF23">
        <property type="entry name" value="SORTING NEXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08628">
        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="4">
        <location>
            <begin position="341"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="28"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="91"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="222"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="455"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="523"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="576"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="644"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="923"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="991"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1115"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1264"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="262"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="651"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="929"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="994"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1008"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1118"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1155"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1265"/>
            <end position="1288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1297" mass="133337" checksum="68D95612008D0BB1" modified="2025-10-08" version="1">MDFQASATRSQPHAGSTAAAATAISNVRKSSSNVELGSFTTGAVSSGGGDGGNVSRSRSGANTSSGGGTATLNGVFAAAAGVAAAARYEVGGGRGSSHVDQPGAADSPTQPRGLSSSYHAGMDRPQSPLHSRHPSGGHPTSTSNFSEQMPLDHRGCSIGADSGKAVAGKHRRSCSYDSVAPRTGGAAVSSDTGAAAIPAAAGGGPSFSLRAPWFDFDFGGGLSLPWQKGGTEPPPAQQRQSDQREHQHQQQSRGGRTQSPQRIHDGGTSHEQHYPTHHHHHHHHQPHHQHEKFNHKHNRHQHEQQPAHHADSRSVAAATADYRGHDRSSSGGGGSSWCRANVAAFEMVSEPLTFGLMAEYVVYKIRVADVVSDQEWTVARRFRSFEAMAGKLAAALPRYASLARALPPKRLFVHTADVTFVNSRRADLDTYLQQLLAVRDLAECRVLREFLMPDQASPTPAKRSGGGGAAPAPDDHSSSSPLLTSSMAGLELPAAKPVVWPAAAARNLLERVTLDLKGLRRRTASRRTSVDLRPPAQTQQPSAPGMGSSAGFGGNGVGTGRPFAWLARRGDNPAAMAVAAAPRPPLPDSADCDARAAAAAEASRGESLPPVSCPDEEDDDAFDDLLDEGEVLVSEEQMPPLIPSEAAAATGPPPSSAPPPSQGAGGGGFRFGLPSWASSANESGSGGFFAFPSVQARLRQCMEGTAAAGPGGGRSTVSFATIAAAVSSSRRGSPERMGMSWSGGGTASGSESAQAVGVTMRLMAALRGVATAAAPAAPVDSPAYFDAQPLPLTPAASESPTSLRATVAEEVTVAAAVASSTPRQSPPSASGGSGGIAAAAMASAAGSVRSLLSQPCPLPAAGAVGSTAACLPAPPVPVVPPLPLPLRPSIEAPMHQMHSASVTSVRKTAAATATATAGDGVTKAESVAGGSGPATGTNGGAAAASPSTLMGLSLDRSAAQRLQSVTAAVPPGTVRIFSVPNSPIATHTVAAAAPAPPPRLPSPPLYSNAGGHDNTAANGGGGGGAALATAAAGVSAPLYELVDVLFGLQGQGFFRRQVISVTRQGLSLFAGGAIDTWVSMRLRDLTSEHSLARVLLLLHGALFPGGAWYSFGLQPQTQQHQQHQQHQQHQQHQQHQQHQQHQNPNHGPQLKPPQQKTPPPPPPATPRWRPNAPIRADTYLEVGTPPPDAEQLAEKVRARLLSRSPAMLVNLVGARTFTRALSDVWGLAQSRTMVLQLAYTLLITLLLRLFPELEPQLRHLQLKGTGPGAGGARGGGRHAGAGADGGNGGRDKEGVML</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9L6</accession>
    <name>A0ABQ5S9L6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010086</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF105">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1-ALPHA INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="3">
        <location>
            <begin position="165"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="350" mass="38085" checksum="75E3B3B4FAE8E067" modified="2025-10-08" version="1" fragment="single">LGGSRMKQLAPRAEHGSRGGGGNTATITTATATATANANATATASNRNMGDVSVDVRLPLGSLGGSPGSRVPAVERPGLEDFLMSYMVPEQPVVVTGAMEHWPAMTRWGNLSYLEHAAGCRTVPVEVGQHYLADGWGQQLMTLRDFLHRYLLPGLQTPPQPQQEQQAQPRGYLAQHPLFDQIPSLQRDIVQPDYCSLGQEGEVRAVNAWLGPAGTTTPLHTDPLHNLLAQVVGRKYVRLYAPLLTDRLYPFPEGTVNSNSSQVDLDVDLDLVPDSDSNSKTRLADQDSGAAVHVDGGCGCRRRGGRFPGVADLPYLDVVLEPGHMLYIPPGWWHFVRAMSTSFSVSFWWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9Q3</accession>
    <name>A0ABQ5S9Q3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009958</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001226">
        <property type="entry name" value="Flavodoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051285">
        <property type="entry name" value="NADH_oxidoreductase_modular"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32145">
        <property type="entry name" value="DIFLAVIN FLAVOPROTEIN A 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32145:SF31">
        <property type="entry name" value="FLAVIN REDUCTASE-LIKE FMN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00201">
        <property type="entry name" value="FLAVODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="3">
        <location>
            <begin position="429"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="748" mass="79785" checksum="E89210B909CF8C7F" modified="2025-10-08" version="1">MRVSNRHQLREDARSSGKYAAPRLVRGHAFTPAPIQRPVLKKSRAVSISYRRLKSTCLTQATAAPLVTSQMGPAPVPPPIEPVIVDYSIYTVAPNAKMLRGTCRCERLKYEVEYGMKRGTTDNSYLLTTPGATVLIDVPYEAYGDKFVSAVESEVPLTSLTHLIITQLDTKSVPSLELLLARRKEVADASATVVGPLQVVLSNPALRLLQPALGEKADKAHLLTSVQLVVARSGAQVAPCGPGEEDQLQAFLTPTPRWPDLLVVHDPVNRLLFSSKLFAAHVAPSEGEALDENGWEGFGDDWRYYFECMLAPSARQAAAALDKLDLVPARRRPGTAPKRVNKTSAALRSFFRSLLRLEKVAAADAEGPAPATTAGAAASEGVTPGSGPLPVCMLLPMHGPGVSSAVTQLLQEYRKWADEQIKAAATTCAAVFYASAYGNTAALAQAISRGITKGGVAVETVNLELASLQEVSEAVSRSQGFVLGSPTLGGHMPTQVSVAMGAILREPSAKAMPCGVFGSFGWSGEAVDEMEGRLRDAGFGFAFDSIRVKFKPTAKDLSLCEESGRALAQSIKKRAKTKELSAAPGTRALNASGPQLAMGRVVGSLSVLTAKDEDVTSTMLASWISQASFDPPGVTVAVKKDRTIEKLLVVGGKFAVSMVAEGRERPIMKALSRPFSPGEDRLAGLDTVPSPHLGVPLLKTANSFLECSVVTRMEAGDHYLIYAHVLDGKVLDENAPTAVHHRKVGNHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAF8</accession>
    <name>A0ABQ5SAF8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000032">
        <property type="protein sequence ID" value="GLI66424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="273" mass="28419" checksum="99DDE7B6493343A8" modified="2025-10-08" version="1" fragment="single">MDAGTPPLPVTPAYFASCCISGGAIAPAPIANADLAQAVETDASISDASGCFPSSSFITSFVGNTPFPTVAPKSGQPVGGHVKQKYRLVSPLFWLIPPVAAFSSEGGLLCTDSVHALALARAAAGRRRMMAALEEARRRFSHLSRSKLPNSWPSTAPSISTAAQELQFQREGSSREGATGSRCLDEGSEMQRPMVAPPARLLPNLPLAPRDVGILSVGYAASIAVVPCEASKSVSKVPETQQGRAGEYGRGNQEGQYEMGGRDGGDSDGRQCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAP4</accession>
    <name>A0ABQ5SAP4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KAP NTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13849" checksum="A0E5649C819B1F8A" modified="2025-10-08" version="1">MMCLGIITVSASTDRHPYFIVILVDDLDETLDSSSELVMQAIKRLLATCVHLATCDFRARGQLFRNIPGSYILFKTFEVGVTYTTHFVSTPICCPSGSASSAASWVTTPISFTTFFQAQNYIYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAZ4</accession>
    <name>A0ABQ5SAZ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphoribosylformylglycinamidine synthase</fullName>
            <ecNumber evidence="3">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Formylglycinamide ribonucleotide amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Formylglycinamide ribotide amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_010788</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the FGAMS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000042">
        <property type="protein sequence ID" value="GLI66819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.750">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00419">
        <property type="entry name" value="PurL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040707">
        <property type="entry name" value="FGAR-AT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055181">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010073">
        <property type="entry name" value="PurL_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01735">
        <property type="entry name" value="FGAM_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003672">
        <property type="entry name" value="PRK05297.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18076">
        <property type="entry name" value="FGAR-AT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22689">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109736">
        <property type="entry name" value="FGAM synthase PurL, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase N-terminal" evidence="15">
        <location>
            <begin position="131"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="14">
        <location>
            <begin position="274"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="13">
        <location>
            <begin position="535"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="FGAR-AT PurM N-terminal-like" evidence="16">
        <location>
            <begin position="754"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="13">
        <location>
            <begin position="938"/>
            <end position="1073"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI66819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1415" mass="152315" checksum="92BFAE9CAC0949A5" modified="2025-10-08" version="1">MQLRAPLRSSVVKGRLGSSIRVPAVLRAISHVSVGKVSMAVLIKQGTHAVYRNRRTVVIPKAVLQEPPQASSPIALQAVGSGIVQHLTAALPGQVVQLYRYPGLSESTVNTLLRKTHEKVTTAITRIDGEQCYNISLTKPLNAAEAETLSWLLRETFEPALLTASSQLPASSPTVTVVEVGPRAAFATAFSTNAVSICTSVGLNQVNRLERSRRFMLHSTRPLTDAEKATFAALVHDRMTEEVYRRPVRSFTEGLQAPAPTFTIPLMAEGRAALEKINKEMGLAFDEWDLDYYTALFRDDMKRDPTNVELFDIAQSNSEHSRHWFFRGNIVIDGEPMNDTLFNIVKTPWKKNPNNSVVAFKDNSSAIRGFQVQPLLPVQPGAPSPLAPQPRDWDLLLTAETHNFPCAVAPFPGAETGAGGRMRDTHATGTGSIMGAGTAGYCVGNLRLDGYGQPHEDPSFVYPDNLAPPQQILIDASNGASDYGNKFGEPLIAGYCRTFGQRLPNGERREWLKPIMFSGGVGQIDHRHLEKHAPEVGMLVVKIGGPAYRIGMGGGAASSVPSGSNKAELDFNAVQRGDAEYSQKLWRVVRSCVEMGDRNPIVQIHDQGAGGNCNVVKEIIYPLGAEIDVRAVKVGDETLSVLEIWGAEYQENDCLLIRPDKRDLMQSICDRERCFMQVIGTIDGSGRVTLVDRNAPPGTPPAVDLDLEKVLGKMPDKTFNFTRSSNKVEPLSLPAGETAASALHRVLRLPSVCSKRFLTNKVDRHVTGLVAQQQCVGPLHIPVSDVAVFAQSHQNLTGLATSIGEQPIKGLINPSAMARLALGEALTNLVWARATALADIRASVNWMYAAKMKSEGAAMWDAATSLRDAMVDLGVACDGGKDSLSMAAAAGGETVMAPGNLVVSAYVGCPDVTQVVTPDLKLGDKGVLVHVDLAQGRRRLGGSALAQAYNQLGNDCPDVSSEILKGMWEATQALVASGQLSAGHDVSDGGLVTALLEMAFAGNCGISVDLPLPSHSADASHGAMGSLFAEELGLVLEVEAGKEAAVIETYKTYGIHASVIGKVSSGRGVEVKVAGAPALSGDVGDLRDAWEETSFMLERLQCAEECVAQEQAGLRDAKAPRWELPFTPVFTPADKLAAPDKVRVAILREEGSNGDREMAAAAFAAGMEPWDITMSDLINGRASLDTFQGIIFVGGFSYADVLDSAKGWAGTIRFNDRLLQQFKAFYNRSDTWSLGICNGCQLMALLGWVPAPGTQQVADVRQPRFVHNSSGRFESRWVQVRIQDSPSVLLKGMEGSVVGVWCAHGEGKCLFPDEQVKQEVLSKGLAPIQYVDAEALPTEVYPANPNGSPLGIAGLCSEDGRHLAMMPHPERCFLTWQLPYVPAGLGLDPKGPAPWIKLFQNARDWAESHRAAAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB18</accession>
    <name>A0ABQ5SB18_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ataxin-10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="700" mass="72250" checksum="9CFF1AB3A9BA6783" modified="2025-10-08" version="1">MPRPRTQRSPTAPASGSAADIEANGNEPTVAAQQDQTIAYRPRDPAQEQILRSLSNTLSREMKLAALNWRGGCFCCPANISFAQCMAGLWQAARKPAESVEALVHWGLNASKFLFEAGPKSSFGRRLLLQAGFTSLVLQLLPLCEEVPQSIALTPQVVFVQIFAIGRRQLPLSTTPQLVVLGLLALATLSPASLRTALLAPAGPQLISALIRALAESSDDRFWSLTFQTLTEWINIKEGDPVVDDDDNDDDNDEAEEEGEQKRDDREATDAGQGGRATTAMAARGALIDALLDAGLAPAVVHVLESSSNAAALRDACALLLAMLAGGVGGGGGASSSWKAVAGSSGRPGAGGHGPTRVQGPTAMELPPDAHLEGQSTCRARVELLKAGLVPALLSALRRRPSQFCYWVAINLGVTGARHLEMQWLMLVIARMSVLPSRLSAPEPSAKCVNAGADATQQAASDPLPAAASGAADSSEVAVIAAVAASSGKGGHPTPGARAQRVRSQRNGVEDPVQAAAEAEQLRLSAVDLLLQSDMLSSLSVESFRSTPVASSAGRGSAPQAPPPAAAEAGIEAEAEVEVEAATRAETGASGACVDAAAAAAAENTATPAAAAESTTTLSADVQAVKLLSRAIAASTLCSCVLPDGTPLELLPPRERNATPDFRALQRDRIRKAAEEARAAQALASANAAVDVLSKAVASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB83</accession>
    <name>A0ABQ5SB83_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-L-isoaspartate(D-aspartate) O-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.77</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_011119</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BTB" evidence="10">
        <location>
            <begin position="565"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="350"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI67197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="765" mass="80785" checksum="C2937D06A99BA23F" modified="2025-10-08" version="1">MANPNAEHDEVIPFEPDLDNDEDPGVPLQGQPRHERLERIALFDFLRLLAGNREPQPGFRTMEELVNHLKSNDTITSDAVSRAMLACPRDLFVPYPHRVEALVDRPIRVEAAGFNISAPHVQAVSLQALALEQGQRVLDVGCGCGIITAYAAFLTGSSGEVVGIDIRDDAIKLACQNLLALKTRNIEFPDVAAPIRLERHNVFIPLKRHLGRYDAVHVGGALPGSRLGALLDLLGPKGGRIVAPISNELRLVTKSPDGSVRQRVLSQVSFSKLEVPRDVDVVLELLQEQAEADRRVEVPSSTFASDLAHLAAPGVDLQTGKAAVMSGFTASASASASASASASASALLPDFAAPSSPRRGTTSGVRPRSDTSLPDDHAASATLPPAYRSSGSQVSRPEEEEAVDAAAADAMEGNISAATAATATATATAAAAAAVTAPITGGWGWKWERPAAKAEGGLNVDMDVDVEAGEKSPLPVRTGMTEDMEAEGLAPAGGLGKANTRTRLPGPNETEAIGEANTSVAVQHLNAERRDDSDGGSNGGHDGDGGGIGLSYLSDVAATLAVGETDCKLEGDGWRLPAHKAVLQARCELLRAHMGSGMRDSDAATYRVPEAVERREALEAFLHYVYKDCLPADMDTELMPQLLHAGIYYGCRRLVRLCESVLARELVEASSQGGADPEVLAAAVAAAGPLLALADEGGLDQLRRVAMQFVMEHFAAVSATEGYRQLPRPLVDEVAREALSRYHGVLRQLERLGEAGSGQPGESDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC91</accession>
    <name>A0ABQ5SC91_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="603"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="862"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1127"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1194"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1464"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="699"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="726"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1134"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1156"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1201"/>
            <end position="1213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1529" mass="155794" checksum="B2D1CDB617B21474" modified="2025-10-08" version="1">MQKCGCVRGWIYHNSLFNGLALTCPGPSLAPACVIFPVLILGDLDPSTLKPFDLQAIYRGCAYPPRHLRAMLLPPQPAAAAMATQGQGSQGAGLHGGGGGSLSQPVGSHRNHHHHQYMSQQPNNRHGRASICGKLPVPPNGRIDRYFFAPNPAASQPFRAHAPLQRRGEAGRNGQWVSGEQLLVGNRHGGGSGGGGNGTQVVTLRGLAVETAGAVARGAHGREGTRKEGGLDEGEEKEEERGYDQRRQGGEDESQDAGFSPRSGKRRRLEVGNGSEVGKTGSSLFARFRMSSAIAVATAPAAVVAMAEQGVAVPEGARTAERWLAASQSPPPPQTHLPQSQPAQDEIFGALRKSAASESRGASAAIAAGGSSSPVTGFHPLLATGGGDKSGGGGEELLLTYPLGSAPDSQRTGLSSQGLARSYWSGAESGSLGPDGGHHMPSGGGATDGSDLDSQLTASGCEGSGWRTWRAGSGGSAVSLLGGRSRAPAQLAMEALLNEPSGGGSAPLSQQGGLCAGGGEGGRGGPPRGVNLGGYGSSRHVQGALEALQRWKQGAGAGACGDCTQLPLDADASVVAGDALLGTGTAAGISIGRAQHGPVPVASAPPPSPPHTGAWGPRLHKPQPPPQFSIYDSDDDMEIDLHRVVSDPDLDPDLVLDTAPERCGVTANGGIRSSRLRPHPNREEASSPLRPPRRAGPGVALGRGRGVGGDSGAAATALPLFGSNDDNYDDNDGGSDDDNGEAEVTSPFKLPACLPKTISPQLQPEVRGCVVGRRGFRALMHRKLRLSGYSLPQFTATTAWSNGAGGAAPAGADACLGSPVPISASRRLNTSIEVANVRTAAGGAAEDQSGVLSGDELLGVDAGRGGGSPPRVETSGKTESDGGLLMAELRTEALTEERTRGDKPIRDRLAGRTAGGSPGCSTILPGLDSGLDRNCHSMELDEMDVGVVGQKCYEGQVGPLYSGDDCGVDGMADAAAVAVGGGGRFDTAVSGGRHGDDDDATRGRLHYYLHGDGDGGGIDDPDVMEFTRALERSEARRQQTQRQLHVGSDGAEAFSAGDLLFPGNAGHAGGGGAGDATVNSGGSGAAAATAESNARAAFQGQGEGPAAPGVTTAAADNPITLCRNLKQNQGMGEEAQRIQEKSKELRNQNQPIAMDQRSEGRHCDAEAETQGPHDGGGSGFSGGCDGGRCQVGSGGGGGGRGADHNGAEVKASRADGGGVDDDDAVHITADGTVVADDGFHSVRHVARFALMAKRVIDKLEFSLAPGPPLETQRQRRTLLQRNRQLLDGAGGTIGWSPGSGGAAAAAVRSRADLSFFRCAPKRRNSTAWGSSAAAVQGKTGPAGPGSCRQSVFVQPVDADAAAAAAGAMQQSADGQMRVDVPYSDDVGRLAGGSGHGGGWEESDGGSDDVMIISEKEAGQPSDGRQAGCIGARFSSLTPGSRGPGEGSGSDRLIRADEMLRRPFQPPRGCAGGAAAVSAAAPPPRRRSVSGCGLRGKTRSSVVGIRSGGREALQHQQQRITDFSRFVFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD97</accession>
    <name>A0ABQ5SD97_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34407">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34407:SF1">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="36196" checksum="2A477E353ECEC21C" modified="2025-10-08" version="1">MSMVTKQSRRNMTKSQLEQATTSSGHFVRGFTGTAAMWLVASTRRYVPVWTGVPHERISDAMYAQLPVAQHHGVPIVSAIDALGPFPTTKLQAWFNQTWYEGDKIHPSQRGHVLLADLVFDYLKMAYEHTRLLQMYDLPKDYIPRQSLFLSQDILDLFLDQTPTTISLTHMPDIVKRFVTPCRGWCAYDDVPEKIGFISNEAGTSCVLSLQPAEVSGLGVGKARVLMYKSYENMGVFKMTVVQGRAGLNGTCEGAYDPNAPVLGSMIVDSMWKERASLAEVAVMQFKLPPASDRSACLLVHLYVVEANPKRLKNKVKLLGISFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDR0</accession>
    <name>A0ABQ5SDR0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="367" mass="40463" checksum="243A96DD05827D17" modified="2025-10-08" version="1" fragment="single">ARLVAKGFAQREGVDYGELFAPTSRASSFRALLAVAAARGLQVHQLDVATAFLNGELKEEIWMKEPPGYESKTPGVACRLLRSIYGLRQASRCWYDKLCTKFAEIGLFPSKSDPAMFVKVDEKGVILALVHVDDMCVAAKTQELVNRIKRAIGGLLSTRPDLAFAISVLSRYMAKPTKAAMGMAKGVLRYVAGTRHLGLVFRRRSSEVFDGYSDSDWAGDMDTRRSTTGYVFRVNGTAVSWSSQLQRTVAASSVEAEYQALSAAVREALWLLKLCEDLGVEASQMRIQVDSQGAMNLGNNPITSPRSKHIDVQHHLVRERVASGEVALKYCCTEKMVADALTKALPEAKFTWCRREMGLDVVETKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE28</accession>
    <name>A0ABQ5SE28_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47570">
        <property type="entry name" value="ZINC ION BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47570:SF1">
        <property type="entry name" value="ZINC ION BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="38455" checksum="0F80D75BAAF461C4" modified="2025-10-08" version="1">MPATATATAGGQPEGTAGCGQRWCVDVLYLGPQAELDQLDTFAVLLPLLPPGCTLRLHMVGPDVPDNLHGAVLVYDGDDLGVTGHPLVAPSPPVQGRCNVTDGAGVAVHHQDGVGCGGRTAGDEAGARFCSDGVPSLSCNEAVSSCTGRHGGLCPRLEVQLYSCMLHEADELLGGVLGGCGGGADCFQMGGCDWTVDGDDGGSGDGSSRVSPGARAAVTHCAVGSNLAEVSKHALASPRRADSSGVRPCRPQQPAPLLVFAPNAGLPVYLSWLPSLELLLAGQDRPVCRMGRSGGAGGGSEQLPVKPVACLFTAYNEEECVRSCQLLEQLFNLRPNVRNLVNPYRQPLCCVERIGNGLPSYSNGFLYGWLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE50</accession>
    <name>A0ABQ5SE50_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RSE1/DDB1/CPSF1 second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011857</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="2">
        <location>
            <begin position="864"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="597"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="645"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="812"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="970"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1400"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1523"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1649"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1853"/>
            <end position="1879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1943"/>
            <end position="1985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2088"/>
            <end position="2121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="368"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="649"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="670"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="706"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="816"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="971"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1400"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1554"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1664"/>
            <end position="1678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1952"/>
            <end position="1972"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="2166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2166" mass="222780" checksum="1E8249576CDC48D3" modified="2025-10-08" version="1" fragment="single">MEEIKVVVEEVEPSTAVYNVVRGCFVRPSGERRGVDGTLGMQLLVNCGDYLQLWEPVDGSLELQQAFYLFERIEHMDLVPASMRLFGLESTDTVLLFTFDGRCALFRLHLQAPSDQLAPGTSPPSTAGPFVLTEIGCLVLPIPALRDGLMPKRLGGVCSSGVAPVSKRVAMTSSARAVVVAAIHMGMLHVITLHSNATTSKVGRAEYGGAKGPATSGASGSSAGGDGSINCDGGDGEPIMFCSAVLIQDTPLYGMPPGDDELEVMAHMSHVYGMCFMPPPERDAFEPPLLVVLHKLERSGGISFTLDCLALEVQFGGKEGGDAAAARGGAWRAAAAAAGGSTGKGADSSNKDAHSGAGGPSGSGAPGGSKTTKPQAIPAAAKAATSAAAGSGPGTTATDDPSPMRATLIPGPWVVRFLHPTTTVITPFPGIPAAGLSAGVLAISSLGVTLFSRPPTLGAIALAAATAEVKARSAAEANPGPPIGDLSGLDPFDDEAVAALFDRWFGKDFLEDAAIEFAETAIQQVDLVQYALAGMPLSAEALAPGVLLIGDSGAGLTLLHAAGSQAWAAQVLPALAGPPPLPCPAMPKVVGYLPRAAGSGDGGRGEGPTPVSEEAGSAQAPEGLLFLGSDCSNSQVWHVPAAHISATAKPPPAPPPPPPPPSPPAPAPASAPITSASGPSSSAAPGTSRDRKALEAGAAKGKEEVQQQQEQQQKQQQAMEDDDVPLPPQLPVLHWPVSELAAIKSLAPVTDIAVVPDSSGADDPWLVASCGRNAAGRLVRARLAAGLQPYMSDGPKVPDGCRMFPLRLENNLCDDSGQQGSSSPGPGHGHTHIAFSFEAADRTDLLSVAGSGVGPVHGAAGGSEFTQVELRGLNHGAASLLVADVAGRRLVQVTSASVRVLEPLFSGGGLAVEWHAPEPLSLAAHSGSILAAASRTRLHILRIDPSTGSVTALSNVKQLQAQLSALALYRLPPPPRPPTGPGQRRPPSSSEPPAPEAESTYILMGQWLTNRVEVAAVSDAARNLLELHLGEDETPRSLALLELSAAAGGRAATLAAALPPVLLAGTNTGRLLMWQLAEGPVGARPWRLGPCYSLRVGQVAVELQPVPPSRSHRLRGGGGGGGYVYVHSHSGALVRPRQQAAAVAAAAAAAGPPVLEQLLEVVRVHGSEGLQSLCHVSSTAMPDSTCWVTREGQLLFGRIDPRVKLRWSTAFVGETIHSFAYHAPSHCLVALCEAHPIGDNSLRVIEVNSLHQVMSMRLAFGHYHTAIAAGPLPCTSGIKAAARRAATMAAQRSGAASAPASASVTAAVPEAPEKVFVVLGSYLSVDSSCDPEVGKHVHGAELQYGALSFLELVAKPDPDGANATQYQLLLQGICLVPHVPTSLAIAQPSLYTALAPGTSGGGRGGGATDGGGSGSPLHATTATSTAAAGMETGGGGGGRGSGSDPVDRPYLLMGCRGGVLLYRVYVDDGHREGDKAVKRALKLVGQTDDLNLPYPKNYAEVQAALEALDKEIQKRVEEEARAAAKAAGAEGDGAVGMNEDGGTKGSGGKRGGEEGEDGEGLAEDESELPEVEVNWEERLKALTEEEAEEKIEWKYGLGLMEQPDSDDEEYFRKWAQEHGLDEAPPEAARAVKLLRKRVQREDMLAAEAAAAAKTAGGTGGGGWDSDDRDDSDSSDDGGGDGHRRRRVRPSIRQLLHDLQRDWSQHVTLVQLDAAKTFGAACVTSLACIYAGPSAAATVGSGSGSGEMSSLVIASDYLGSVTVMQLVANRRGAALISCGPDRHPIYAQAADWLDPEHVLVAVHPHGLAVLRRDSKAEADAMRAAQQKAVAEFEAGVDRQRQQQDVAQVWEAPGAGGVQQRGDARPGGGAAAAGGAAAAGGGDSAAAAAAAAGNSGSRFSLEDLHLLKPQMEAIPGLTMHAACRVRHMVTRMCRARLGLRLQPHIYPDHHQPSRHSSASTPSTSRASTTATAAPGEARRTLAGTGRGVPAQYQHQQWDANDLLLCFSVSGYVAAVRLQHQGPAAATVVPPLPPLAKLARLQHAVAALDPADTALLTGVDSASYSHSYSWPLVIPRQTAAGAGLAGAGSSAAADEAGRRRGAQPPLSASAGTSGAGGTGRSAQMQVDDVEEEFIALEAEAEALATGCVDGTVMRVLQAAAEAQLRKDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEW5</accession>
    <name>A0ABQ5SEW5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_012341</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family. DDX46/PRP5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17953">
        <property type="entry name" value="DEADc_DDX46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22475">
        <property type="entry name" value="KH-I_AtRH42_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056149">
        <property type="entry name" value="PRP5/DDX46/KHDC4_KH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23469">
        <property type="entry name" value="KH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="11">
        <location>
            <begin position="665"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="696"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="885"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="391"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="465"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="7">
        <location>
            <begin position="665"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="28"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="229"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="280"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="465"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="519"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="558"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="575"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI68023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1413" mass="151058" checksum="C8DEDE0A1962B545" modified="2025-10-08" version="1">MGRDKSRSPSRKKHKEHKDKKEKKEKKDKRENDRDYDRGYRERSPPDRDDRRDRPRDDHGWTTRDKYGPEGRDRDRIRDHGRDEYRDRERERDWERERNRDHERERDKDRERERERDRHRERERERGKERGRDWDRERTGERRGRDEIPDTDPGPSGKRTKREEAQGLTDDVAVALGMTVETAAAGAAPGLTAEEAAQQRRDAEAAALEAQVERRRKAVEEWRKKRLAEQGGLEQQQPPAGAGAEQNGTGTAADGEPAAGGGGDAQEAEGTPTKPKGWSLEDDDEDDGGGAEDEEDIPDADADEGDADVEGSRPPPPSAIKRPADDDDDDDPLAAFMAGTPFKGPSAGKPMPAKAVLTKPVPGKPVAAKLVNSDGIVKKGLLIAARPAAVAADAPAVKQEPGEATAAEADTARDMEGAEAVATAKPRRRWAPVAAADTMEAEDDEVDPLDAFMVAEVLPEVNKLAEQEAERRAASEARELSPAPLSTMPAGATDMEMEGAKVGMAAVPSQQVKLEPEVSHLPGPSSRLSPPPVAGPSARSNGTGAAAGPASLRSGSIQRRKPRAAVSRRRHSSDEESSEGSDLEEGGEEEMDDAEWARLVTSGKLSKGEKLVAVDHSTINYPPYRKNFYIEVSELTKLNEAERAELRKDMDGIKVRGKNIPAPIRAWTQAGMSSRLLEVLKKRGFDRPLPIQAQALPIIMSGRDCIGIAKTGSGKTMAFVVPMMRHIKDQPPLQQGDGPVALVIAPTRELVAQIAKEAKAFGKPLGLNALAVFGGSGVANQISELKRGVEIVACTPGRMIDLLVTSNGKITNLRRVTYLVLDEADRMFDMGFEPQIMRIVQNIRPDRQIVMFSATFPRQVEVLARKILNDPVEIQVGGRSVVNENITQFVEIRPEKERFHRLLEVLGEWYERGKLLIFVDKQESCDNLFRDLLRYGYPCLSLHGGKDQSDRESTIADFKGAVCNILVATSIAARGLDVKDLVLVVNYDVPNHHEDYVHRVGRTGRAGAKGTAITFIGPDEERYAPDLVKALRESGAAVPQDLQALADSFHTKHKAGLVKAHGSGYGGSGFKFDTNEEERFRVDKKAKAKAMGLEVEGEEDAEEAALDAAVAALEADDIRPISAAKAAANAAAAAAAGGPINPADSVSAVVAAAREKLAEAAKAGLIPAPSAALVASVTPSAGGATQAQPAAGAAAAAAAAAAAAASNPLLAAAQQVAAKLTAQVTAGVPLSATGPLPLGVPSGFGVAGTAASGSAPAAAPLAPAPGMSGISADALARAAAIAAKFGGTISLPGAAAAGATAGRPGAAAAPLTAGFEVELEINDFPQHARWKITHRETMNQINELTGAALTVRGTYVPPGRPTPEGERKLYLLIEGPTEGHVKKAKAEIKKIIEETTEKVMRRDAPAAGRYSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF69</accession>
    <name>A0ABQ5SF69_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_012199</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22906">
        <property type="entry name" value="HFD_DRAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF5">
        <property type="entry name" value="DR1-ASSOCIATED COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="11"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="89"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="228"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="294"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="378"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="496" mass="49889" checksum="8FA297F5ED335206" modified="2025-10-08" version="1">MKTRKICPLATRIKKMMQTDDDVGKISQATPILIGRAMELFLEKLCKQAIVVAQTRQAKTLTPSHLKAAVKADTTLDFLAELVAPAPDLPAAETENGDAAPKPSKRRRTNDNYDAVASPEGSGRGRGRGGAAATSGQASDDTPPGPPAQGRGSKPPLPPGSQAPSSRGAGSAKKESSGRRGPRGKAAAAAATAAAAPIIEGPEAGPPDAAELTASVLHMPALPVVQVPPSEAPVPGPSTSSGLAALKGQETAVKHEATSRQDPAAAGSSGIAASGGGCSIVAALRAQQPFFGLGRKGKNLGNKSSGGAITPSSTGTQSHPPSLHQQYAQHTSPQPGLEASVCATTAEATCMDDRRQLQMARVLPVKLEPLDRSASGTFAAAQDAASSRYGDHGQAAQPSPFGHRGLSSGAAGPTMTGTGPEVWGGEDAGMAPGQLSMVGSSVGPLAATAGVMMSGVAQVPHAVAGVGTLMAPLGASLAFFQAPQQQGDVDDDYDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF96</accession>
    <name>A0ABQ5SF96_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05483">
        <property type="entry name" value="retropepsin_like_bacteria"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034122">
        <property type="entry name" value="Retropepsin-like_bacterial"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="682"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="862"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="742"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="868"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="980" mass="101741" checksum="8F07BAC843F9E3F2" modified="2025-10-08" version="1">MQRTPLSGGSLIRGAVSRRAVLASLPWTSLPHYRASKLCTNALARGDRTGNLQSLALEHVLEQLRKVQGIYRLAPNVEATVTGRGKHLGIAVSWNLRWTASGCFSEEIRGPQLTFKWGYDGREDSGCWEVDSSGLVRIMECDDHEAVLMTTYIRTGCWLQPHLKDRFDMELLPKPLISVTATSGSSSSSSGRRSAVPNGGAVAERGDTRSNAPAENSNSSSSHKQQSTIPATAATVTATATAAPVRSANDRGGGGAGGHEPATAATHGSSQSGATPALDDVIAIGLRLKGRKLRVRVLVRRRDWRLLGFDHRMCADVETWELGEWKEWAEGVQYPSSALHKASNGGQHAYLTVGGAAEHVAPPPQLPEVLLPPAIAGAAAAVTASSSSLSPSSPPLGSTAINDSSADTASSPTSRSSPNVFAVTGLTAALQLPRPTDFGMPLLPPIPGDTRYYLQGGKEAVPLWRSASGHFLVRPTINGQPGGGYFVLDTGASGFVVTPQAAARLGGQSFGETHAASISGKVAARFVRMSSWSLGGLCIQQPVLMVMALDGLVRGTPGEVVGIVGHDLFRRAVVEVPQLLEVKGGGAFATAGGVVSGSDSGSDCEEDHDTLGVSREREVSTAAGASSVSALYAGGNGSSSEAPFGPPLSALRSIPVFDDVGAAAAALSQPPSVAAAAVPSPTVVQEPSSSPLCFTVPRETHGKPGSHLTPGSSVTAAAEESSRGDTCIHNSRSRFTAGPGASCSSNDSTSSSSSSRNGSGQSLVARASAGRSTTARAVWRAALTVPYTVHEMMLHHPLEYGNESEWVWEPLVMIANLPHVHLCFADPGGGPPRSVLVMVDSGACGADLMLHSRSQKELGLLPAATAGGPSGTAGGGGGGGSSSAGKSQGHYLRGVGQEPRDFVQLQMLDVPWLEVAGVRFEQIRCMCAAIGGLDISIYSHGILCGDLLARLEWAIDYTHKRIGFRRGAFAPAPSSTPLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFE8</accession>
    <name>A0ABQ5SFE8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="2">
        <location>
            <begin position="712"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="2">
        <location>
            <begin position="1284"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="487"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="772"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="864"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="976"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1086"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="800"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="810"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="833"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="900"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1350" mass="142540" checksum="032ED4E2CE73C040" modified="2025-10-08" version="1">MWNKQRLQLRRKADGVAGEAKTILVDVLHGDDRFNITWKLDGGASVLKQRHIPKSKLLQIADVVSSGPLHTLMDDIDTCAMNAAIKLYRNQQHPALSEKPANLESVAQSCSGTASGNPRCKPLPAHNDQTAGPGETSQSALLSTDAVGSAGLGPGGRSGGSAAVAVAPAAFPLLSAAPAAAVTPAPSPRIPTGCERNTSSAAAVRKTKEVADGVPHSEMTIAALPSPPQRPSPSRLQSQSLQPPSSPSDGANNEAAPLLMPLPSQQQRRQQHSAGVSLSPFNFIGVTLPPSSDRSDQTVGVQRPALLDGATNGCCPGGSVDAPRDVSPRLSSPQSPLPAGRHANDCTAAETMRTQQAQAVTLAATMLADDNQVHVVTEGATSGNGEATAVARGHGLTGHIARQHQRGTRKRRSREMEMADFARGRQQGVAAAAVGAEEEGASLHVAGDVCMDSGCCCCCCVEHGERAVEFGAAEGYPGRYASLGSAGCQGAEEGGNTGQHHHHHQQQQQQQQQPGRQEGEEEVRKTMRDSANIMATHGMEVVEEQVVMEAQAAAAQTAAVAAAAAAAAVQGAQELMGVAAAVQQLPGTNTAAAAGVEEATAAAATQPTQSGFEGEAMEAEAHVMAAQVAQRTVEAAGAAGRAAYMQTAAARAAAVLAAGVLAVTTAAEDATYLICPFTGRSFGRSLCLKPGDVPLAEQLSDPRRTVAANQDREMEEIVNETFSRRLGSSVFLIKWLGYELDPGEPGGRNGHWVSQAVLESWVPMMLAEWRLRGKDSSRQKSGADGSNPMSEEEKKKEEEKKEEEEEERKEEERKEERKEEKKKEEKKEKEPTAEEEEEKEEELTTQGLHLSWIGDVDISSQDLQDAQFTPPTSGRLDRDGNSGRDAPLTGGRNIDEGGSHHHQQQHHHQQHQQHQQAVEVVPPAGYPAGLDEDEALLDINDLLTRDGGEDPDGWQELLCRASRGRPAAEAPVGSVIHTNEAGDGGTAADPSCSRAAAAAAPPAPPAAAAAIRDDVAPVTHLGPIPLHVDVVGAGAARPGPSAAGPSGCKIQYCKKRPARQQPAAERDKPEEQQHLNDELVFLGEDHRADGRGAGQRILPSQSRRRSARIQAYLESMQSTGGQQPPSQPPPQLQQQPQQPPAKRVALQWAPNGGTAAAHTAIPAIAVDTSAAPVILGVSTATTATVLMGATATAAIATATTATATATNAATATATAVGRRKCTRRCRHGPFSEEVEARLKAEVSSGSFVESMSTGRKYPKSLCCGPKDIRVADYDPDDDPQSAVGAVERIMDEVTDDVTGRRYLLVKWKGYELDPGGEHHQGHWEPVGHVPRKVRARVLWERCKPFWFDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFZ0</accession>
    <name>A0ABQ5SFZ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dCTP pyrophosphatase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11537">
        <property type="entry name" value="NTP-PPase_RS21-C6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025984">
        <property type="entry name" value="DCTPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14552">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14552:SF21">
        <property type="entry name" value="DCTP PYROPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12643">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="26197" checksum="F8EB48A20C04E6D6" modified="2025-10-08" version="1">MNGNRDQSDTDDKEDNHYLHKPSDFQQTSQDREEIPSRLKSEAIEMQQEAAPHVGGRSASAGEQPPPCTAASDARAGQPEGSHPGPFQGHPPRPWSDVTLEELRGRLAEFARERDWDQYHTPRNLLLALVGETGELCELFQWRPEAEAGRGLPGFSHQERQAVEEELADVLLYLLRLSDMCGVDLGRAALSKIRKNASKYPAAKCHGSSAKYTVYVREQGQRQEQSCPQEVHEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFZ2</accession>
    <name>A0ABQ5SFZ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lipoyl(octanoyl) transferase</fullName>
            <ecNumber evidence="3">2.3.1.181</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_012675</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LipB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16444">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00013">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020605">
        <property type="entry name" value="Octanoyltransferase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010925">
        <property type="entry name" value="PRK14345.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993:SF7">
        <property type="entry name" value="LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993">
        <property type="entry name" value="OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01313">
        <property type="entry name" value="LIPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="7">
        <location>
            <begin position="231"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="134"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="466"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="475"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="495"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="594" mass="63685" checksum="14E759E690D50000" modified="2025-10-08" version="1">MALPSTTSKPARLLMDQNVAPSRPVRHFFERAQNGAASRQGCKTSLARPISWIPYSPTNAIGDASCNINRCSYSTTATGCARRGDLIPIRIQHISRRIDPWVYRITVRADVIACSASSRYRCLSAATCRKTWSRFPSSTESPEHTAVERSHGSVTLSPGRRAQSTGITEAVQTAAKVTFGLAATAGAEGSDPRVIVVYDLSQEVVDYEQAWAWQRALLDRAAAAAADEGTRGCRNAVLLLQHPPVYTLGAGSTTDHLRFIPDNSTIPLYRTERGGEVTYHGPGQLVMYPILDLQALKPDLHWYLRQLEEVVIQALDAVSGIRGERLEGLTGVWVDGAKVAAIGVRAKKWVAYHGLALNVVTDLSPFSRIVPCGIADRPVTSVKKVLAERQRALDPFAPAEDDGAFGTAMESRGGGSGGGGGGAISGYSEMMPLSQASQDDLRRMLARTGMTPTTLKSITSYGAEGGTAVPELDDPFAPLSPSLSLPSGDLGQDAQVDDAEAATMTPDDDDVDVDVEDDDGDGEVDVDVEDEDDDGDGRDGRGTEDVGDHVNIRSCWTESEHLLLREYGCALLVAFANVFDLELRSGDPGDLKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG16</accession>
    <name>A0ABQ5SG16_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_013412</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039558">
        <property type="entry name" value="TPA1/OFD1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051842">
        <property type="entry name" value="uS12_prolyl_hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117:SF0">
        <property type="entry name" value="PROLYL 3-HYDROXYLASE OGFOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13661">
        <property type="entry name" value="2OG-FeII_Oxy_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="109"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="237"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI68866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="446" mass="48938" checksum="68FD322827655B19" modified="2025-10-08" version="1">MGRRNRKVSEAYEHWMNDYRVASSWIAKKQLERAVDANGGLVRLENFLPEFVAEGILATVGAVSDEQWNDTSASTDYRQNNISHSFSSVKHARGLDAVVRLFSLVRPGSLNAFSAARYCKTDHIAPHDDRAYTQVKLDSGRIITASRTLAIIYYLTRDWHEEYGGVLVDLEDPTAPPGVGRKYVPLWNSVVAFRVPRYHAVTPMTTNRPRYSIFGWFLEPGRLYELYRGEDAEAGKKAGREGAAQHQRRAGAKRKGGSLGIADDSATAPGPETERAAGRAVNAAVKSGEAVTLQTGSGGQHEVSQEWLRTNPNQHGPRVKRQRNADTDGTAVGPSGRSSAKQRQPRVHRQNSTRQDQQQEEDMMGQECGPSDVIQSAVLGTVRGHGGLRCEAVGDTAIGDQVGMAGGGRQRSSVGLAPGFLIDLGRVRAFRLPGRWQGRRKHMCAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGA8</accession>
    <name>A0ABQ5SGA8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="279" mass="29567" checksum="3D7C6F7F7B9A228D" modified="2025-10-08" version="1">MDTSICCQASASAGFSGRVQMAWPCRHRIAARILASSREEIARARQIHNTASVLSSQALIGGQHQGRTREALHLAALSDTTILPGGTGGSISESTSTVELRQTLAPAAVEAVAPSIAIGDSVRGAMDSPEGNLTGGMLPSAYSSFEDLDAFDSSTSGLDSDEPDMCTLVADSSGDVQVVCESDPTHPLSLAEAAVYNPPLPPSLLQDIEAKLEGDLVTIAVTIIFAVGFISLEFGVNDLIDHYFGDSLLSDVVCVWVGLTVVFWVKLSKARLMRFDRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGB4</accession>
    <name>A0ABQ5SGB4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Starch synthase, chloroplastic/amyloplastic</fullName>
            <ecNumber evidence="6">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_013534</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 6">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Chloroplast</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Plastid</location>
            <location evidence="6">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03791">
        <property type="entry name" value="GT5_Glycogen_synthase_DULL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02095">
        <property type="entry name" value="glgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825">
        <property type="entry name" value="GRANULE-BOUND STARCH SYNTHASE 1, CHLOROPLASTIC/AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825:SF2">
        <property type="entry name" value="STARCH SYNTHASE 2, CHLOROPLASTIC_AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 6" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046260545" description="Starch synthase, chloroplastic/amyloplastic" evidence="9">
        <location>
            <begin position="20"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="Starch synthase catalytic" evidence="11">
        <location>
            <begin position="241"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="10">
        <location>
            <begin position="542"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI68997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="731" mass="80794" checksum="18D90019B8D73A5F" modified="2025-10-08" version="1">MPVRFVGLNLLTTLPRAVCVSPSGRVNPSSTSETMQSSLPTLVMSKSPGLSRPGVSAPAGRLGITQNSPASFSGRCHKRSHVVYAQDEARSGNIGDIHRQLLAEIEERRRNQAELLEKLNKLASETSTKAGNNAGQAPVVSTPLPVPAMATVTSGPPSTAAQPPLATSHKPIPTPSPLPASSVVNVPSAPIFEVPSLQMALDSEMYIEPAVPMAEALLLPEEDVPHPMQKAPPPAGPNVMNVVMVGAECAPWSKTGGLGDVMAALPKALARRGHRVMVVAPRYENYPNAWETGVRRIYSVFNSQQEVGYFHAYVDGVDYIFVDHPSFSSHGKNIYGGERGEILFRCALLCKAALEAVWHVPCGGITYGDENLCFIANDWHTALLPVYLQAHYRDYGKMTYARCVFVIHNMAHQGRGPFDESSMLELNDEYRERFRLYDPIGGEHMNVMKAGLECAHRLVAVSHGYAWECQTPEGGWGLHEVLKVNNWKLRGIVNGIDAVEWNPALDEFLKTDGYKSFTIETMVEGKRACKMALQRELGLPVDPDAPLLGFIGRLDYQKGVDLIRDNYDYIMGEKCQLVMLGSGRQDLEEGLRDMESRNKGQCRSWVGFSVKMAHRITAAADILLMPSRFEPCGLNQLYAMAYGTVPIVHAVGGLRDTVKQYNPYDNVGTGWCFDRAEPNKLRESINNALYTYRQFRESFHGIQRRGMEQDLTWDNAASIYEEVLVAAKYQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGE0</accession>
    <name>A0ABQ5SGE0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="639"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="649" mass="64090" checksum="BDCB815E026E6941" modified="2025-10-08" version="1">MQVANGVMDVVPGGMAPGNFAGSADQIQLHARMAGSRNRTLSNGFPYVMRIPSTSSGEVRLGFGADVTTGIAPTGPGIMPAPAELLSSRPTFTDCLSSIGLPGEESATFIPLIIEPYEPAVSTVPATSEEPDVPVLTTITGTASVESPPSPLTPPTAPPQPSNSPNISPMSTPAGKAASSTAMSASPIIAPRPPPGSAVQRVNSCRRNVMRQISNASSSVAATVVAAAAGGGGGSSNPALPSAPKTPPPAAAADFPSLMLPPPSPVNCTGEVLVMGIDSAAVSSVMAMAVPGCSGGGGGGNFTDGNKATSSFLDPSKPAVRRLLPPLLRASQAFHDSATIMDALPVAADDCGGSAAGCNPAPLTSRCSSSLIVGGSNTASPAGGASALTVGALEVWAELQDATAATMTAAESVPLSPPPPLPVDVYDLPSPRGRRALPPMDPLRTSALSATAEAISELDSSPADLTPATQPELYNMASPPRPLDVSYALQSGAVSGGGGRRSGTQRLPWESEGPRTWGVTASEAGEIAPAGGGGGGGSASVGGGCGGGGPLPLDMPRLRTWSAITTEVPLTALTGGGGFSGCGSGTNCGGAIAGGGPSPKERRQLNRANSTGLATTRMPVVAAAVAAPNAPLPPIVDRGGIDRRQTTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK21</accession>
    <name>A0ABQ5SK21_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014452</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_uS10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001861">
        <property type="entry name" value="PRK00596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01049">
        <property type="entry name" value="rpsJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="4">
        <location>
            <begin position="81"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="177" mass="19460" checksum="94AB909D638AED2C" modified="2025-10-08" version="1">MQAVAGRCSRAQPFTSSRPVVARLHSGAVVRRPARPSQNVAANAVVSESGAVDVEQYEDELDLPMTAGTPLAPAGDKVRLRIRMRSYEVKLLEDCITQIQTVADATGAVFKGPVMLPTKKRVYCVLRSPHVNKDAREHFEIRTHHRLVDLKNLSAETVSMMMQWVPPSGVEVECSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK62</accession>
    <name>A0ABQ5SK62_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PITH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PITHD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045099">
        <property type="entry name" value="PITH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175">
        <property type="entry name" value="AD039 HT014 THIOREDOXIN FAMILY TRP26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175:SF1">
        <property type="entry name" value="PITH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PITH" evidence="2">
        <location>
            <begin position="15"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="215" mass="23678" checksum="7326A4A8311E9B6E" modified="2025-10-08" version="1">MPPPSKGCCAHDHDCEAADCGIAYSLYKHISIDQVRCLNEQSPGSCRNILRPWHTRLQPLEQPLRSNQDDDEQELLLHIPFDGAVKLKGICVISRGAPGTAPARMRAYVNRDDLEFAAVSQLPAVQEWELVADGDPRGGIEYPTQVSRFTGVHSLDLLISGASGGADYVEVTFLGLRGEFAERRRQAVETVYEARPVPGDQQRIPGIGEGPHWHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK85</accession>
    <name>A0ABQ5SK85_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_014486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="36"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="323" mass="36602" checksum="82372BA591B9F04F" modified="2025-10-08" version="1">MEPPDGVEVMRPSKRARSDTVLDEEVALSQQHEDCENQLLSLPDGVLHHTLLFLPANALTTLNSVCRYFKKLTHRVAKEKLVTEVGYKQAARWRGYNWLQRLAIEETATRFDLLRGEAQRFMFTEVTNSKAVREIRLEEPGPRLLLSDVSTAQAPILRWKLQLRGNNAAEFGVVPLDMQDHAKALHKCMPNTADERATGFSSAITVGSLLPARLPVMKNTVVEILATPREISFIVYNPEDGAEMIWQNNSTVSRPYTGPRELRLQLPNNFKCPVKLAVTAWQRAAFDVLHMLSPQELQQLTQQQQPQQQPQRPKGVPRQRGVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKJ7</accession>
    <name>A0ABQ5SKJ7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045672224" evidence="2">
        <location>
            <begin position="22"/>
            <end position="3664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="2658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2737"/>
            <end position="3648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="2497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2504"/>
            <end position="2609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2644"/>
            <end position="2658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2743"/>
            <end position="3063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3071"/>
            <end position="3101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3102"/>
            <end position="3116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3135"/>
            <end position="3173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3180"/>
            <end position="3193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3201"/>
            <end position="3240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3241"/>
            <end position="3275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3276"/>
            <end position="3291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3306"/>
            <end position="3317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3318"/>
            <end position="3327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3328"/>
            <end position="3338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3339"/>
            <end position="3354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3361"/>
            <end position="3370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3417"/>
            <end position="3442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3443"/>
            <end position="3470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3485"/>
            <end position="3517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3544"/>
            <end position="3589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="3590"/>
            <end position="3646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3664" mass="370915" checksum="2FA304EC7FD5F186" modified="2025-10-08" version="1">MRIGQALAIIAATVLTNQAVAAGFDVWLYSNLSVVASQKRIERIKEQITFATSGVLPSTSTTQPNLYGAIGYVIPATRGSYFEVEQLGEDLEKLIALLDFVRAGSALVLLDGYQSIDGSNSFVQLLSALLGSDPRCTAADVTSNMLMHRRTNAISGLDNAIEVKPGLGVSTLSCETGQSIYSAYINQVEYAASMMWHVGSGTVCWIGSSFFKPNLRGFYDVMVAALVDTAGELLVPPSDPNPPAPLSPKPPESPRPLPPPPPPPWPRPPPPSPSPPSPPPPRPPPPSPPPHSPPPPSPPPPSPFPPSPPPPSPLPPMPLAPPPSSPSPPPPSPFPPSPPPELPPPPSPPPPEPPPPSPPADRESPSSTLPAPSPPPPPHQPSSPQPEFPPPPSPPPPSPEPPAFPPPSPQPPLPPPPSPFPPSPPPPFPPPPNPTPPPPPWPSPPPPSPLPPKPQQPPRPPPRPPSPSPPLPKPPSPLPPPPPSPRPPPRPHPSPLPPSPSPPQPPQPWPPTSPPRHPSPSPSPPSPPPSPTPRPPSPFKPSPLRPSPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPSPPSPPPPSPPPPSPSPPSPSPPSPSPPSPPPPSPPPPSPSPPSPPPPSPPPPSPSPPSPSPPSPSPPSPPSPSPPPPSPSPPSPPPPSHPPPSPSPPSPSPPSPSPPSPSPPSPPPPSPPAPSPPHPSPTPPSPPPPSPPHPSPTPPSPPPSSPPPPSPSPPSPPPPSPSPPSPPPPSPPAPSPPAPSPSPPSPPPPSPPAPWPPPPSPTPPSPPPPSPPPPSPQPPSPSPPSPSPPSPSPPSPPPPSPPAPSPPAPSPSPPSPPPPWPSPFSPPSPSPSPSPSHPSRSPPSLPLPSPQPLSPSRPSPSPPLQSPPSPPPPSPPTPAPPPPSQPPPSPSPPSPPPPSPAPPSPLAPSPSPPPQAPSPSPPSPSPPSPPPPSPPPPSPSPPSPSPPSPSPPSPPPPSPPTPAPPPPSQPPPSLSPPSPPLPSPPPPSPLPPSPSPPPQAPSPSPPSPSPPSPSPPSPSPPSPSPPSPPPPSPSPPSPSPPSPSPPSPSPPAPPPPSPPPPSPSPPSPSPPSPSPPSPLPPSPSPPSQSPPSPSPPSPTPPSSPPSSPSLPSPYPPTPSPPSPSPPSPSPPSPSPPQPPSPTPPSPVPPSPPPSPPPPAPRPPSPLPPSPLPPAPLPPPPSPYPPWPSPTSPSPPSPFPPFPLPPSPSPPSPQPPFPSPPSPNPPSPMPPSPSPPSPWPPSPSPPPPPSPTPPSPVPPSPPPSPPPPAPRPPSPLPPSPLPPAPLPPLSSPTPPSPYPPWPSPPSPSPPSPFPPFPLPPSPSPPSPQPPFPSPPSPNPPSPMPPSPSPPSPWPPSPSPPPPPSPTPPSPVPPSPPPSPPPPAPRPPSPLPPSPLPPAPLPPLSSPTSPSPYPPWPSPPSPSPPSPFPPFPLPPSPSPPSPQPLVPSPPSPNPPSPLPPSPSPPSPSPPSPSPPPPPSPTPPSPLPPSPSPPSPPPPAPRPPSPLPPSPLPPAPLPPLSSPTPPSPYPPWPSPPSPSPPSPFPPFPLPPSPSPPSPQPLVPSPPSPNPPSPLPPSPSPPSPLPPSPSPPPPPSPTPPSPLPPSPSPPSPPPPAPWPPSPLPPSPLPPAPLPPLPSPYPPWPSPPSPSPPSPFPPFPLPPFPLPPSPSPPSPQPPFPSPPSPNPPSPLPPSPSPPSPSPPSPSPPPPPSPTPPSPLPPSPSPPSPPPPAPRPPSPLPPSPLLPAPLPPLPSPIPPSPYPPWPSPPSPSPPSPFPPFPLPPSPSPPSPQPLVPSPPSPNPPSPMPPSPSPPSPSPPSPSPPPPPSPTPPSPMPPSPSPPSPPPPAPWPPSPLPPSPLPPAPLPPLPSPYPPWPSPPSPSPPSPFPPFPLPPFPLPPSPSPPSPQPPFPSPPSPNPPSPLPPSPSPPSPSPPSPSPPPPPSPTPPSPLPPSPSPPSPPPPAPRPPSPLPPSPLLPAPLPPLPSPIPPSPYPPWPSPPSPSPPSPFPPFPLPPSPSPPSPQPLVPSPPSPNPPSPMPPSPSPPSPSPPSPSPPPPPSPTPPSPMPPSPSPPSPPPPAPRPPSPLPPSPLPPAPLPPPPSPYPPWPSPPSPSPPSPFPPFPLPPSPSPPSPQPLVPSPPSPNPPSPLPPSPSPPSPSPPSPSLPPPPSPTPPSPVPPSPPPSPPPPAPRPPSPLPPSPLPPAPLPPPPSPTPPSPYPPWPSPPSPSPPSPFPPFPLPPSPSPPSPQPLVPSPPSPNPPSPLSPSPSPPSPWPPSPSPPPPPSPTPPSPLPPSPSPPSPPPPAPRPPSPLPPSPLLPAPLPPLPSPIPPSPYPPWPSPTSPSPPSPFPPFPLPPSPSPPSPQPLVPSPPSPNPPSPLSPSPSPPSPWPPSPSPPPPPSPTPPSPMPPSPSPPSPPPPAPRPPSPHPPLPLLSPLLPPASSPTPPSSYPPWPSPPSPSPLSPYPLFPLRRSPTPPLPPPPSASPLSTPAPSPSPLSPSPPWPPPPWPSPQSPMPCSPPAPSPPSPSPRPPLPPPPPLPPPLPPPHSPAPPSPAPPSPFHPSPSAPSPPPLSPTPSAPLHPYQLPVAPIPFFLQPTEPRSPPPPRPPIRRRSPPLPPPELYPPPLEYIPPYDAPAPVEYTPPRTSPLPLHPSSMMPPPFDIPPPMEYIPPAISYLPTRPSYMVPPVTLSPPSQPNLVPTWLPAVPVDLLESPPTPAPFPLLPSPAPPPLSPQAPPSPLPPSPIPPEPYPPRPPSPPKPRLPPRPPRRPPTLPPPSPKPPLLPPPSPNPPPPLPPPSPPSPLPPSPIPPEPYPPRPPSPPKPRLPPRPPRRPPTLPPPSPKPPLLPPPSPYPAPPLPPPSPPSLLPPSPIPPEPYPPRPPSPPKPRLPPRPPRRPPSLPPPSPKPPLLPPPSPYPAPPLPPPSPPSPLPPSPIPPEPYPPRPPSPPKPRLPPRPPRRPPTLPPPSSKPPLLPPPSPNPPPPPPPPSSPPPPSPPFPFPLSSPPPPPPPPPPPPPLPVPAFPYSTPPSSQPPRAVPLTPHLPLSPPPCIPLPPPPPCPPLSRKPPEPCPPLAFPSTPTFQPFFPMSPKQYLPPPLSPYSVPMHPLLQPPSPLPLSPTPPLLPSNGPPFPSPPSLLLPPSPPPAPTYSAPRTPRPPSPPFPPRPPPRARRPPGRPPRPRPPPPFSPGPPLCPPVPSPPCPTLSTPPPCPPVQAPSSLSSQPPSQSPPLSASTPPSSVPFAKSPFSRSPQPPPAAPPSPSPPLPFSHSLAWVHTQTSLPQPRSPESASPSAPSPPSPLPWDPGLAPSFSWPSSPPSPAQAPSPPQPVPFTDSSPSPPPSPLPPQLLSSPASPQPPQSSTIARLLASPSRPPGLPALTQRPSPSLPAPFAPDLSSRSPPSSPQPRRSPILSLWPEPSLQPPVSPLPLLSPPAPLVLSLPEPPSSRPLAPRHTSPQAPYMQPPVSPLRTLPSPPPPVSPNTPPPPSPQPSSPIPSTLSPSPGIPATQLYPQFSSPPTPSHRSPISPSLPLPAASSSPPFPQPDVSSTESSSPPSSFLSSPVLSSPHPPRSQFPPRMPLAPLRPPMPPWRPPSLPSPPLAPPSPPVRKPPRLPPSPILPPLNIPLNLSRSVASTLRRRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKS2</accession>
    <name>A0ABQ5SKS2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="432" mass="44277" checksum="A6D26B18FB675B6E" modified="2025-10-08" version="1" fragment="single">MFAETNVAHSLALTPSGKKFVPVIATAAMETYNVIGSDRHLIGVDMNSENNDKMDYDNSSSAVLPGIPSGAQTDACEPIPASCSSNASASMPLSTFSVTAQSLRLGASSLCRLGVHSVRSGSQHLLGSPDHLPSQMMLHAYPYTHHPHDFPCCSPPRVLHQLSVSPLLYRPHAGIPVHITNCSRCCYGPITECDDGGGVGTGKGAARDIGADHTGSFALRNRFGKDAVILVEDLSPRVCFPVITPGGGRHEVVQATATAMSVAASDCDGTSAPTAAAAAVVADTARNFGVRLPHEKSLAAEAVANAAPPVAVAVIPGPLPSRADAIASIHLGVAAMAEKKESGGEERAAEAATPYHGAFAAAATAATAATSNPIAPPITAAIMADVTEMAEIAASSKCGYQFLGDGAEPSPESQTRLLPSGFPLRVLHPTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKX5</accession>
    <name>A0ABQ5SKX5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vesicle tethering protein Uso1/P115-like head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015203</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024095">
        <property type="entry name" value="Vesicle_P115"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006953">
        <property type="entry name" value="Vesicle_Uso1_P115_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013:SF0">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04869">
        <property type="entry name" value="Uso1_p115_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vesicle tethering protein Uso1/P115-like head" evidence="6">
        <location>
            <begin position="653"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="577"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="849"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="916"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="986"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1049"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="862"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="995"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1233" mass="125471" checksum="09AD56A09E877E1D" modified="2025-10-08" version="1">MLGRLAKGVGGLAKGVAGVALGEARAALTAVPNDEAEVEGLLQRISGSLLPEYRRQAASQLKDLLVDNPKAQMAFGSMGIPVLMAVLRDDRDDVALLQGTLEALVAATNGGALASGHERHEQHGHQAAHSLPPGVTAAEAHAAALNADLIAREPENVALLLGLLEDEPVGVPDFYVRYHTVQLLTALLSHCAGRLQEAILATPMSVVRLTELLGERSEVIRNEALLLLAALTRPDPRVGSPSGGSEVQKIAAFEGAFDRVAELLREEGGLDGGMVVQDAIELMNNLLRGNMANQRLFREMGHAAMLPQLLNAAVPPASASVGAAGVGSGSNSASPGAAASSGAQFAQAAGPIPFLNVGGGAAAAGHGLDAADEPSLELHNALAVLAAAGSDALLGPRQLSRQKTANMLGLMETLRLLCARTVASADPDRANEQANRAANQAKLLAAGALDALLAVALREGGVPSAPVRAASLGVLGDLVEGLGPAQERLARATVRPGQTLLAATATDVVDGSPAAMAAPPAAANEPVPALAAVLRVVLYSVDATERSAADHVIACYCRGNADGQSALALSIGPLAEPQQPQLHSHHHPYGSTFANGSGGIDPTQTTHGLFGQDMVSGLLAGGSALGHGGAVGTRLALGSGGGSAGLLVTCRAAGVLQHLICDNASAKERLLTTPLEPPPAPGVPPDLLMPRLMRYLTAALRPSWGDPARLLVCTLLRVILVWLQDCPPAVAALLDGAAHVPLLVDLVIGRQGPAAAPAAGTAAADSSGNAAGGDPVVCGLAACVLGACILYGKSPAAAAAGAPLSQSDLILDVVMSRVGLSLFFFRLDDLRRSPVFAAAARSPHLQLVKPSTRTAAAGGGADAEQQQQQQQAGGMATEGSDANGGASSIIFDLGHEAMGLVASIEESIRQRTMETFSRPAGHRAQDAVPSSAAEVPGPDDSARLGWALLHLGRIAREVDGLRGRNRALAEDLFRLSQGLGSAPAAPPPFGISVGPSHPHSHPATHSVAATPGSLQGPHPGVQAHSYPHQPGAVSISQPVATSAADVEARVALELRAGRAEQELAMLRQQSEVMAARLAQAEADATAAREVAAAAHVAASKAEADLADLSGAYNNLEAHAFATEAQLRTTQAALAAAQAAAAEARPQVAALEGQPHGGSGPSEADIQRRIAEAAAEARAEGKAEAEEGMTDLFVCLGQEERKVEVLSKALEGLGVDAESLLASIQDEEGVEELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLB4</accession>
    <name>A0ABQ5SLB4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="700"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1041"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="550"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1067"/>
            <end position="1078"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1236" mass="128844" checksum="CB46B2B8392E95C2" modified="2025-10-08" version="1">MVYESPAAAGRPMVCRKHSPWTMERDRKKSKAHLGSPEPLSEEVEEEEAEPDDHEMTEQEAEVLACLGELNVESSVPNQGDPDGAMLSRAGSVPDGQRRKLRGSRALSASYHNRLRSCMLNEKGSPLPHSTRSFTNHTGGGQENTGPILFPSVAFGEHSKCWLGRRPSPNNKDSSGAHSLQHQGATGITLNPSFSAHGPVLGTAADQSLLAAAALANSSRHDKQLPCDSPAPKGRSHRRSLSGASIAAAGGLNDSAEALHGPSAESCVLGQGQAVQPGTWPHPRGMSEGSAQRHGATFKTGGATNCDQGSGFNTQACVNSARDGLLNCLPDQSKPLSLASSAATSAPLVSLATVAKFGIAAFGVPASVRSCFQDSLDASTEHVAGDISAAAGPLMNVHELARCAMSCPGDMCSAAAAIDMIDRGIGHCASYGSFTAGLDAVPTPTTGHVRGPISELVIEPHADKSSEPLISGHMRHGTPFAGTGSGPGKALADSAEELELRRLTHQQPHTPTVRPELRQHTGLHPHQQQVELKQQQQSLRFGISQSRPVSSDSATAGTSSALSAAASEPLPVEPSNPTFVPFDIVSLARSAGAVCFGISDFTTTFATPGEYLPDALQAAIDRKPPSSLWPATQAGDCHNLDSRPVSDGTDINSSKPDGACRTLAWLGSYFASNTACTSPETQIGPPDASAAVAAGTEAVEHAGNGGSGHDLGGDVRCDQDDSPGSGDGEDGESWLRTSQQAPDPDDPSSAGKYSAVLLRRLQAAAEHEELYGTASGDHLVACGFYHLQEGGGSGLSHGDYGLRGSTGRLHLGSHGSSSMEPSPLPRLSSRLRRLRVSMCLGGGGSSGPLTSPTPSLLLGTRSRSAGVTPGGATCRRLRRRSDLLMPDQLGQMSARGHADVDELEDSDINAAAGVDEHMHDVSHQLLAAGGSGGRLRLMAGRSGDRPAHAHHHRGGVQLLQARHVSGNGLLFSAAPGLRSEPMQRMFSPNDTDFGMAARVHSAEEQLVGSQYRLHSHTGNDFLNGLMDYEDESVEYTTPPHTPLRGIGGANDTDATTAPHAALAPKPSGASSAEGSFFAIAPGRGRPGLGKGLLHQRVTFAGQRLGGSEVQPASPPAAVCNGRGENGSSCAQLLMSNALETTQDFNRCLALAATSNDRGKAERLWQEMCSRGVTPDGGTLNLLLRCLARSAADPDEAQLLAREVCRRGGLSLNATAQHLLEEICFRFEQLNGSTLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLH4</accession>
    <name>A0ABQ5SLH4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="31761" checksum="7BDF3C2E60F7DBD6" modified="2025-10-08" version="1" fragment="single">LIRRLLVAALALHHQKHGALNLEAHEGAVHTILMNLFIQHKQSAQWKGAIEFMHVSKSGGSSFCRLAKDNGCRSQNFYRKNCLITDFNDEPRYFDAKVHKQLAGGAKTRCSKGLKTITYRSKELICHDRRIRLQKKGFTMYANEYTALGGRMDPALAHACPNMLTVLEIRHPYSRTLSHIKHAWEWYRIYCGPDRENVYFAKSGHNTTRWWTALMPAPTNNYLVRSLLGEKVFRLPQGGITRDHLALAMNFLAEQYDVVLVLEDQLQFKEGMRYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLU8</accession>
    <name>A0ABQ5SLU8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_015164</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="373"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="52"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI70384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="654" mass="70562" checksum="3831C239AAFCF071" modified="2025-10-08" version="1">MADNILSRPEPVVEASLSGSQRLADRLAGGLGKGIRQIATDLVQLFLKDRSHRNSKRESRMGSQIRSTRIPHVRDGAGAANPGFSQQHSRSSLPRLSEAEPEFKLIRDALSDDGSPSHFTSTKQRIFRTPSRAHQMPITESATSPAASTRGASKPTSAADKIDQSRCAHTNKTPPSMDIVYKSMPSFISLGHAILRAASCAQPKFVSALPENVSVLEGSCSSGNTAVAASFSVGAASSADGYLGSGAAPLPAAPSPPLLPAAKDVLIPAATSLRHLRQELLDEPTSQIQIPKSTDKGEGKIKTAYDSHANAQGEFVVVRMADPNGCCTGNTTATADATAGGRRPPPVGLLYSSPTLPTKMRRSSNLQWSVCDFEVTRNIHKGYSGRVYKASCLYSSSDVVLKAYNLAGLSNFLRNQVLRELNIHSRLRHPGIVHLLAAFREGNNLVMVQEYIRGGSLERLRRKLGGRMSEFQAMHLVLMPLLGVLTHLHERGIVHRDIKPDNLLLTENWKLKLCDFGVSVCLREERAVTRTGSREYFAPEVDCCPQKQLPEDNKDNLELSYTAAVDVWSLGALMYELLVGFTPFPGGPPARKAGAQGSAGAALAFPAGISQAARAFVRDCLQLDPSDRPTVHQLLEHAWVREAMVAAEALIAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM76</accession>
    <name>A0ABQ5SM76_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="399"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="423" mass="46145" checksum="ECA5EFB689E42725" modified="2025-10-08" version="1">MASTACGKSTGRPLIVGKRTPVGVCLKRFQGLARRSTSREEWTQLDSSTPLPTESRYDPSLPFQTAVQRLKELLEAASWESEEEQLRYEVANLVPEMKLTLFTNLGSVLTLAAFVTSWLTGEDPLGGFALRDNSLGAAAVGAGYALPLVLCSVVSRLTPVRHNFPVLDDLQDSQQEIVKPIVEDLNASQLLILASVVVVPSMLMLLPAFHGALTVAGQILTADLVPPLGMHLHLPDVLRRQAGVLVPAVCSAYFAAWVVTRQLDVNDRQVTAIRDALESADRYFLHAAAERAAERPDILQRQATSQQQWPSDATGLATSFASVGGASHGLVDEAIRDTPYNGSYSYGPLRQLGAEMSQAFKTVSILWLMTRRKAARLAYVLTALNVVYFGIIWHQTRDLGTPVVAAMLATLTELFLIKHFPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN92</accession>
    <name>A0ABQ5SN92_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021503">
        <property type="entry name" value="DUF3110"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11360">
        <property type="entry name" value="DUF3110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="323"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="402" mass="43616" checksum="D9F3E026A00C6A42" modified="2025-10-08" version="1">MLMFQRQLGASQNGLARQSSSELAASPCALSRGGSRNNSFSRRAPGAAGHSSLPHSRSRTSLLVACNTYNGQNGDISANDANTPKEIQSQAYGEPKAQSYPQQQPKAHSGNSNSSMRSPRDAEPNAHPPLSSSAFSNYPTRPLNSEQSWDFTPEPFVPSPANLPTNEDEPLLYPPRPLFTWTSQPGMDLFRVCSRTWNRLLQVFVILFGVGERETEGIYSLRAFGSDGTPHETIIAFECEEEAQRYAGLLEASMDHKPHVCSIPPLELLSFCMDQTYACRLEPKGSLLIPPDFNVAITDWERSLRLRDGKFAVLDVEPDRRSGGSSAAAATVTATAPPPPAPTGSGGSNPHPPSSAYPQQHQSGFPNLQEVGGRDPSTLARYSGLSKSELEEIRARLEALLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ63</accession>
    <name>A0ABQ5SQ63_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidylprolyl isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46873">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46873:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045198801" description="Peptidylprolyl isomerase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="24859" checksum="9A97CF208CFA701B" modified="2025-10-08" version="1">MMRAFDLALFVGLASLGFCAGDLARQDAFITYSEVSRMKNERNIATVAAVSLVSTYGQIRLRLRRDWAPQACEAIAAMAAGPLRACNGCSLYRHEPVPQNWGVSGFNGPPYALLQGGMPGMTNKVKMENPKMLPVLWGTAAFIADTSDFFIGTADHREWGGAFTVFAEVLPEDMAAVVSRIPVEPYRNSTDTFNITTRWLIQPIPFTLESLRLDEIPTAAPLHEASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ73</accession>
    <name>A0ABQ5SQ73_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15173" checksum="9C37EAA329A63E47" modified="2025-10-08" version="1" fragment="single">GQQDSTRRLFLATAFEGADMACDDPDEETPAPPTKKPRAASVRPGITGHEDQPRQVKGAGKSKRGGGQQGRHLAPKGGHVQKKGPKQNLAEYLTTFKCTRREFHFRKQHGMCLRCGSDQHRVDSCPMLGAEKNKGKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLL6</accession>
    <name>A0ABQ5RLL6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000245</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039994">
        <property type="entry name" value="NO66-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13096:SF9">
        <property type="entry name" value="BIFUNCTIONAL LYSINE-SPECIFIC DEMETHYLASE AND HISTIDYL-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13096">
        <property type="entry name" value="MINA53 MYC INDUCED NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08007">
        <property type="entry name" value="JmjC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="5">
        <location>
            <begin position="271"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="81"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="455"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="750"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="455"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI58509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1162" mass="120415" checksum="8E7DE96D1F1C6F49" modified="2025-10-08" version="1" fragment="single">CASALGEVALDMASHVRPPVASVHMRDSGGGSGRPGGNSSSSTCPGRSLLQPTEWLHLLEKHWETRPLRLNQKWRRVVQRRRPPGAGGFSPANISPADHYGGHLGAAEAEHTPSGFGCDDEGEGDDDGSNWTPLETIRSVMQELLPASCHCPLMDTPDRNLVPYVRRVRSHLLPYMVARADVDLIRSGSDQFVTGAAAGGPAGPAGAAAGTWVVTATGTAGNLGASGDAAGVPAAVQPSPAPPVLATPNAVTANDCGAAMIRGFTVVLRAAGARSSKICAVQDQLESLLGLPAGANIYCTPPGKQGLVAHYDDHCVLVLQLQGGKEWLLQPPRLLPTQLLPLSYCPRLPLAPLQATAMQNEPVVFATAAPAEVTGPAFHSSTDASEATGMTMEATGSPAGPSFFVGREEGTAKMAEPAAVTTRVELQPGNLLYVPRGWGHQAVAMAMAGAGAASGSIQSAWNGDDNNGDGGGANVSDPSKLDPGKAAEGALPSGISLHLTFGFEVEALFTWQAVAHCILALVAAERRQQWQRAAWEADGHVQDIRKEHRHQLRRNDLGGDQAEAEANSPAQSVPPDATLAAQVLLHTWLVQTAFSARTSPLLLLRKAAPLTALARLGTCSQLQQGQHHQTQQPPKHVTTTATVTAVADTGSCILPPFRLSPPAGGPTLATRGPLSAALAAALADAPTPVASAPMGAGEGVHAAVDPAGMGIGSATGGGQQEGGKADEEERGLKTVAAMALLRNLQSCLQRGGVGEKGPESPPPLQRRKAPQTALLTEQPPLKPAVTAPIPLEQVRFSRAGVGTCGTRATNKEDLQGGTGGDLLLGSDDVAVEPDTDMDLHLPCDCVANPELAGLGARTPSEAEPLTVLALTRRLMQLLLQQWHQQQQDEEVEQLEANTQGQTEGPRGKLVCKIGEALRRDIDEVECPGVEGVAAGRCSPAQGQGQGKGQGTGLKSSVAAFRAAATSATVDAAGGMPPHVSCGPYDAPYWLEWTTELLSKAGHSCTGVAPGWQLDCTLAGVETVADGREGEVGALSEEGNIGYNRLGPGHGMKLHREDNAGGHEDEYCDASGSLDAVEVCKDVMQAIVSGAASTAVDTISGNEDANPWIEAVLHEAEGLLATASSCGDKVAQLAVALVATDQRDCLRARLRARHALLALHVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMR2</accession>
    <name>A0ABQ5RMR2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoribosylaminoimidazole carboxylase</fullName>
            <ecNumber evidence="4">4.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">AIR carboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_000616</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + H(+) = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + CO2</text>
            <dbReference type="Rhea" id="RHEA:10792"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.21" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016301">
        <property type="entry name" value="Ade2_fungi/plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004679">
        <property type="entry name" value="PRK06019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609">
        <property type="entry name" value="PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001340">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="179"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI58850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="647" mass="67963" checksum="AE7320122950C187" modified="2025-10-08" version="1">MLLNGRNTKITPRAAEVAGITSTVPQPNTRHTATAKRHKAAQRARRVSAIVASAGPVMEKDVTSVSATGLPRTAVIGVLGGGQLGRMMALAAANMGVRLKCLDPTPDAPAAVAAEHVQGHFRDAEAIEDFVKSKGVDVLTMEIEHINTDALMQAASDVKVDIEPSPATIRVIQDKFAQKQHFAAHGVPLPQFRNIKCRGCMEGTGKAFGYPFMLKAKRLAYDGRGNYVVRSEADIDSAVAALGGFEAGLYAERFAPFVKELAVMVVRSRDGKVVSYPVVETIHKNNICHVTEAPADVPPAVQAKAREVAEQAISCLEGAGIFGVEMFLLGNGTLLLNEVAPRPHNSGHYTMDGCVTSQFENHVRAVLGWPLGETRLTSGCAIMLNLLGEADGDEGVRQAHDLMAKAYNTPGCKVHWYGKDGMKVGRKVGHVNILASSREEARAKLGALDPAGLAALQRSSAAYAAVSAASATDASGGRPLVGIIMGSDSDLATMKAAAAVLEEFGVPVELTVVSAHRTPERMFEYARTAHSRGLKAIIAGAGGAAHLPGMVAAMTPLPVIGVPVKPSGAHLDGLDALLSIVQMPKGVPVATVAIGNAANAGLLAVRILAAHDPRMLDRMLAYQQGMTDTVLGKAAKLEEVGWAAYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNG5</accession>
    <name>A0ABQ5RNG5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_000378</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Endoplasmic reticulum membrane</location>
            <topology evidence="2 10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the OST1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007676">
        <property type="entry name" value="Ribophorin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049">
        <property type="entry name" value="RIBOPHORIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04597">
        <property type="entry name" value="Ribophorin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044982910" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1" evidence="10">
        <location>
            <begin position="22"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI58641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="602" mass="66954" checksum="E4A7922A11CA1900" modified="2025-10-08" version="1">MVRLTDLLLASLLALASSVLADNIKLQQVDRKISLNSQFVRITETVKVKNVGDQVVSEVILCESLGGDAVLSSYKVVETSPKNVLAVSRATVAGAPAEAACHSVNLANPLPAGETVGLTVTAVLAKAQIPYPAELGQHETQLMLYNGNVYVLSPYSVSSQTTEVILPTSTVKSYTDEKPVSKSDNKIKYGKYDLIKPFTEKRLSVHFENNKPFKHILTYVKEIEVSHWGNIYVEEKYEIKNAGAKHTGSFSRLKYAHMNLKANSFRDLKAVLPRSAHSLYYVDLIGNISTSNVRKAPTSTIMDLELRYPLLGGWKVDFTIGYSVPLKGFLTSAKSGQRRLTMDLSSPLEDVYIEDMVVRVVLPEGATNIKANLPYDMEKSFDIKYTYLDTTGRPVLVLHKQNVAAPEHAAKFSVDYDFNTVHILREPLLLVSVFFGLFVAVIAYNRMEFTITRDDKWEAARSKEVLATYMEQIQALLEDEATILSGLARATIQLREASEVDAVQREKTTVEKRLKDLEEKVKPLLAAVESRSVRVGSQVRDVLEKTRLLQSRYFKQLSDRIDLLKKGIALGEISRKLAPAQDALTAAKRELEGTITTVFGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP62</accession>
    <name>A0ABQ5RP62_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_001095</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15873">
        <property type="entry name" value="R-SNARE_STXBP5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF25">
        <property type="entry name" value="TOMOSYN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="5">
        <location>
            <begin position="1390"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="351"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="783"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="863"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1074"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1245"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1355"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="807"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="833"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1074"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1254"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1287"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1408"/>
            <end position="1417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1455" mass="153738" checksum="DC86758F99F4F465" modified="2025-10-08" version="1">MFGFGAASLKKKDGKSFAEVQTDLLRADALASADRALLCSSGIPGTADALAYEPVQGLVAISTSDGKVKLLGRPGCEVTEYSTARYPYGTRQLLFLLNRGVLVRVSKGGFMESWSVVSGNPSDAGVRSLGTVKVRGDKICCVADMTRNPYVLLGCASGSLRIAMLVDGLGSPTTLALQARGFIVAPYRVRPHEMRGEGEVRQLAVQSFGPIHRALVLFTAGMVAVWDLRAVELTTSINPNSADATDYVQALADAGVATAVCWIGTDRGDFATGHVDGSVLVWVLPGLDPGKAVVAAAMRVSSGTSEPVRMLRCVFGEVDGLLVLGGQEVEQPEALTWIPLLDGTDEEAMLLEEDEEEEDSSDGDEEEENEGGRESGHSVKGRSSQSRNRHDKGAAARQRSKRALFRLPWFGHLIGFSLVADGGYVTGYEPSAAVLQLVEGGQLVLYHLKDEQPHMVSPSFQQQSSITITEAPLVPVRRPASCSPNAITLTSLRKVASEKGDEYWGGSLADVGTFSCGTPPSLPSDATWGLLYCTGYKDGSVCLWDLHGGTTRLLCTAPAGNAVSELKRTSANSGSVTCLSLVWPAGLLLAGHHKGEVRVYQFSSTERSVDCITMESIDTPGVAHSLHQPAGLHLRLRVQVSSGEITSLAYCQAIRSVAVGDKAGGVALVDTAKPLVRWYAMPAQHAVLACSLAPVPLPPSRLRVPEVTGEEGTPSHAVFIADSEGRLAALDAARGCFIGRNGELSPKNRSYTLMLELLDENFVPIWSRRQVGAGCDQAALGGFTSGLPKAGRRSQMQGVSGDEGDDGSCHAEEQRSQRGEGSRGQGRSKAGDKDEDNDQDSEGSEEDFEFMDAETLLAKAAMQVEGAGSRRTSRRHRSGGGTRSREPDSSSPPAVLSSCPDPAAHYVLLVTDQYLRLYSSSNVLTAERSTLAKRSPAAQQQLVYARPIQVAGAPGVMALAAADHGLCVQVYSLPSLELVHETPLSASLSWFWDVPPGRERRLGRLAATSRLGHLLLLGLGNELLTLGLATSLPRPPPPVSLFDPAAATASLAACAAYEDEHQVAAAGRLLRKTANQGPHSTGQLQGPPSAFGAEAGPSVTFAPEMIEGVDSPVVRTEGRRIDKAPGATAAAAAANAAKTAPRVAAERVAGVAKEANVTLTRVFNRVQQGFSKAVEETTRGVRQLATNVEKGVATMIDGGASGLTAAAGNAPASHNAEWYASLPDLSAIFLRNIPEDDQTLARLRQRAQGRGGEDGDDDDDVSILTISDEDDGNESSRGRAAAPRNEGAGTTAARAARPPPQTHAAPQPSAAAAAAQSPGIHGYWPHSSAVGRSAGGEEASLRNELFAGGRPYVSAGGAAAPSSRPRHAPAGITHPHTRTADEIKRAYGRQPATSTARANEVRSVMEQNRSKLEERGEKLRQLNDKAADLENSAAGFAEMARQLAERERNKKWWQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP91</accession>
    <name>A0ABQ5RP91_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="465" mass="48726" checksum="22DE05E84B6D5A44" modified="2025-10-08" version="1">MAHNSNFCYHNGFSGAFRGNAFHNIIAAATKSSKGFGRARKPEEHKSKRDGSLRNDNKDFKEVAASIATHRPVKPEEAARGKLDYVQVEDWGDGDRAKLGELRLVSHVQQYEGGASTAMGPGNSDIGGSRPFHTQLAHQLKMAEARGALAVAGPPPPPVTEWSFREARYAQYLADQLEVHEALEAALLEATAGATTTTTGDNGGNSSSSTRRGDAAAIVTAAPVPTLQDSVQALLQLVPGRLGLERSAAIRRDLAALRATAGHPAAVAAARRCAAGTTSVPESAAAVASEADPDAEGRAASLVASIPGVGVEGRRGESANTSVVVPSPLSASDDERTAGPMARSYAQLLRRLGRAAAAGDDEQERQPSSLRLLAHAAVLHLVAQAVWVRLGATATERLALLQRGAAATYHEYPGEVTDPRVSLSRALDAAGEHLRSPAARQVVFDEVPGAMQKTGLLMSALAHRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPQ4</accession>
    <name>A0ABQ5RPQ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thiol-disulfide oxidoreductase DCC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001431</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007263">
        <property type="entry name" value="DCC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044691">
        <property type="entry name" value="DCC1_Trx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34290:SF2">
        <property type="entry name" value="OS04G0668800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34290">
        <property type="entry name" value="SI:CH73-390P7.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04134">
        <property type="entry name" value="DCC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22021" checksum="EDE36AB211EF95CC" modified="2025-10-08" version="1">MRTALATARLASPLVTHRLVPLPKRFVLVGANSTRASSSTASSAESAPRPSWDIRLLYDGDCPLCMKEVNFLRGRNQAGKVLFVDIASPDYDPAQNAGVTFEQAMVTIHGITADGRIYTGVEVFRLVYEAVGLGYVYAITKNPAVLALANKVYDVWAKYRTRLTGREALEVLLERRRLDLAGKASCRTVVDATTGSAKQCDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPR3</accession>
    <name>A0ABQ5RPR3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05271">
        <property type="entry name" value="NDUFA9_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF11">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="55"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="395" mass="43504" checksum="E185640040A85CA1" modified="2025-10-08" version="1">MLPVLARNAAGSALARLAGLRWVAAASSNREYSSILTAEKHGPGGRSSVSGIVATVFGSNGFVGSYIVNELARRGSQVVCPFRSTENEAMHLKQMGDLGQIVLLPELDIRDDEQIKRAISRSNVVINCIGMRLQTMNWSFEDVHVDFPKRLAKIAAESGHVDRLIHFSDMGADVDHASKRMRTKALGDEAVRAAFPSATIVRPADIVGIEDHFYNYLIYQLTLTVFAPVVETGSNKVQPTYVLDVADAVAAALRKPETKGQTYYLGGPEVLTIREVYNLLLKTMRIHRDDTVHLPAWAVKAMYKPMDAVRRMLPGLPMTSPLATDDYVEESLRDKVVPADALGYADLDIVPQKVTDGLAIEPVRHARVGGYRWGDMATVAKDVPESVRKYYNIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPR8</accession>
    <name>A0ABQ5RPR8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="802" mass="85580" checksum="C09E5E8EFFFFD487" modified="2025-10-08" version="1">MRKLSLSLLTRVGHCRGSSLATSSAAIENLEKAVAASDCCSTSSIAVNWGGHGASAGFATQAWRTAHRYNFIQPVDTNHINAVLESATSLDHAAIPVLQYGAWFDPDQVTRTLQRLPRMLRYQRRKSSRAAADRAAVTSWRLMDVLGSRLAQVAVDCTDEQFTRAMWAFGVSRRHHAEAIHVACEQLPRRLGSLTMSQLATTAWSLAAATSSAPKPAQEPVRDALQAIARHLVATRPANLSAPAPPRAQIITGAAATPVTGGKDGAAAAASVRVSPAADVVAAAVAAGRAALDADQPWMDHRSALKLAWAFAALGVHDTQALDAIGDAAAARIGTQLQVHDGASGPLSPRATFLYWTIRGWQAWPRPRPPRARTGGRPSRYMRDERPRVVLRDFTLPSLAPLLGAFRRLGYRHEGLLQASAKHIVASAGPSFRVAPADLTRVTGAMEALRFRNAPALAALLGSSRLTALPAPVLTRLTALAAEWGVPSRHSGGLFGRLARQLVARAWIDSNTAGDTTSTVAKDIVFGSVGADAPTAENSAAVAAAAADEGTAAPQLDTLAKESWERQLPSQSWRTDVSSLEREADLWQTTHRRPEDVCQPADLARVLLALAKAGFKESEPGAPLVGALFARVWPHLTRLSGDALAGLTLAAAHYGPYMDRGTIPGLPVSHLEAIQEATRTLLYRATTDQSEGLRSLLSLKVLIGKNTDEAEKRTANTANEAAELRQKMELSAGGALRLATALTLRPDAVTDKELRAVLSVLDVESTERKQLGELIGRMRRKSRNSVLPPRLLVAVGEVPQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ11</accession>
    <name>A0ABQ5RQ11_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transaldolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001593</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF40">
        <property type="entry name" value="FRUCTOSE-6-PHOSPHATE ALDOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0704">Schiff base</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="35472" checksum="9927E30B3F62EDEC" modified="2025-10-08" version="1">MKGPNVFKLEHKMQHQMAYSSLMRKGSPSGIFFSGRTRLRSRCQPCRCERGYPGAPNSAIKHFDKKTDPFTNLRIFVDSANVTQWELCLSTNLFYGITTNPGILEKDGQRCTLSNLTRLCRIGEELGVREMQFQAWGDSAQSMVSVALDLFGMNPNLVVVKLPCTLEGFQAAALLRESAVRVTMTAMYTSSQVLLAQSVGAEYAAPYLGRMNDAYGNNQGYKEVVQMQRILTTQKARTRLLVASIRDAFTMASLAAEGCDTFTISPSVASKLFKVTYTLDAVEQFEAAARRMGAYDLQGSATTPTVHLHSHPHPAAAAAASSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ15</accession>
    <name>A0ABQ5RQ15_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(+) synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="3">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_001162</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07570">
        <property type="entry name" value="GAT_Gln-NAD-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="10">
        <location>
            <begin position="6"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="788"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="788"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="814"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="843"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="896"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI59308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1032" mass="113294" checksum="496EE15BDB9A5DE2" modified="2025-10-08" version="1">MVRRLATLATCNLNQWSMDFEGNLERIKESIRLARARGASYRVGPELEVPGYGCEDHFLELDTVTHSWEAVADLLSDAEDLTRDLLVDIGMPVIHRGVMYNCRVFLLDGQVLLIRPKLHLANDGNYRETRYFSSWKKQGGLEYHRLPDVVVKAMAQRAAAAAGHRKLMSPRPVRVDVPFGDAVLKLQDAMLAAETCEELFTPQAPHISLALAGVEVISNGSGSHHQLRKLNQRLDLIRGATAKAGGVYLYANQRGCDGGRLYFDGCACVAVNGSLVAQGGQFGLAEVEIVTATVDLDEVVSYRCSISSLREQASTTLTPAMVYVDFRLCGDARPAAAAAVAAPTAVAAVTVDGPMKVVAATAGQARLPYATRPITPKVLLPEEEIAYGPACWLWDYLRRCGAAGFLVPLSGGADSSSVAAIVGAMCQLVVAAVRAGDPRVIADVRRVAGYGSGELPSDPQELAGRLLSCVYMGTVNSSRETRERARLLCEQIGAYHLSLSIDSVVEAVVGLFAAAVTGGRRPAFQVHGGTTAENLALQNIQARLRMVLAFLLAQLLPWARGVPRGGGAGWLLVLGSANVDECLRGYLTKYDCSSADINPIGSISKTDLRSFLQWAATNLGYSVLAEVEAAPPTAELEPLVEGQQPQLDEVDMGMTYAELSLYGRLRKVARAGPVAMYNACAAMWRGQLAPQAIAAKVKEFFRFYSMNRHKATVLTPSYHMESYSPDDNRYDHRQFLYNIRFVHPLSTAPCFHQDLLQQYNRPWLLPVRHLSITTSTISPYHHITISPAPAPAPAPAPAPAPAPAPAPAPAPYHQHQHQHQHQHQHQHQHQHQHQHQHQHQHHITSTSTSTSTSTSTSTSTSTSTSTSTSTSTSTSTSTSTSTISPAPAPAPAPYHQHQHQHQHQHQHQHQHQHQHQHQHQHQHQHQHQHQHQHQHHHHQHQHQHQHQHQHQHQHQHQHQHQHQHQHHSTIIIITSTIITSTAPPTPCPVLTQSGTRWPWQFRRIDDLAARDATVEAMEKEAEAGVGPGATTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQH7</accession>
    <name>A0ABQ5RQH7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zeaxanthin epoxidase, chloroplastic</fullName>
            <ecNumber evidence="5">1.14.15.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_001765</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Plant hormone biosynthesis; abscisate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.21" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017079">
        <property type="entry name" value="Zeaxanthin_epoxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46496">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46496:SF1">
        <property type="entry name" value="ZEAXANTHIN EPOXIDASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036989">
        <property type="entry name" value="Zeaxanthin_epoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0937">Abscisic acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FHA" evidence="15">
        <location>
            <begin position="574"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="703"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI59800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="728" mass="77864" checksum="A53F917FFD0E2FF3" modified="2025-10-08" version="1">MLASALNPGSLCSGSGRVAEGHVLKPGVLTVSSRAAFCAHPLQSITTAPSVPQQAARKVVLTCAASAAATSSSVAQLVHQNSKPMKVIVAGAGIGGLVLAVALLKQGFEVQVFERDLTAIRGEGKYRGPIQVQSNALAALEAIDPEVAGEVLREGCITGDRINGLCDGLTGEWYIKFDTFHPAVNKGLPVTRVISRVTLQQILARAVERYGGPEVIQSGCCVTSFEERPKATGGREVVVHLEDGRLFSADLLIGADGIWSKIRKQLIGETKANYSGYTCYTGISDFTPADIDIVGYRVFLGNGQYFVSSDVGNGKMQWYGFHKEPAGGTDSEGTRKARLLEIFGHWNDNVVDLIKATPEEDVLRRDIFDRPPIFTWATGRVALLGDSAHAMQPNLGQGGCMAIEDAYELAIDLSKAMEAANGDASAVNVDAVMQQYQSNRVMRVSAIHGMAGMAAFMASTYKCYLGEGWSKWVESLRIPHPGRVIGRLVMLLTMPAVLDWVLGGNTDNVAPSRVPYCALGDKPKAFEESRFGEFMANDASIVYSSHADWLLVSERPGPAGASDDANSSSECKGIYMGTQPALVGCSGSPAAPMLTVDDVHVHERHAQVWRQDSNVSRNGTVSSGGGGNCAYFLRDLGTGRGTWVNGQRLKEGAVVQLWPGDMVEFGRHPSNEVFKVKMQHVTLRSDELQGSHYSTLMVGKIRESKRSPEDEEGGVRGGGFAGGKLVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR08</accession>
    <name>A0ABQ5RR08_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI60001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="688"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="869"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="701"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="919" mass="93065" checksum="CB7A8A5AB9340EE1" modified="2025-10-08" version="1">MGTDDGSCFGCLPWRRRQVSSTRTTNTSVTKPLRPTTANNGKIQGILKTAPEPSPAKWRSQLGASESSSTASVNGVASESGPLATHNISATALGPADEVLTVAASPQARTRSALGPTQIRFASQLQIHPAPVGSPTASNSSLTATSCQTRTGVTSKTPPPGEEAAFGDPDGPIGNGASVASAPPLPQPPAATTAGGGWSSADAASRLQDLQERFRQRASSTAPRNGDVTAAVAPRPPGIMDHLGSSDQMQCDSFTYCTSVYGRRSSNGGRLGTLPPLSPSPARTGKKNVPMGQAHSRSHGGGGGGGGYTTGRQAQKRDQVLQDDRQNAAQTIYAPELSRGSMTAHIPGSLPSMTQPQASPAATRIYPQPSATAATSAVAVTTRDIAPPMAPEALPLPITPQPPRVSWSEMSVAPVAVAATAAAAAAAQQQQLAVPTGNNPVVRSGKSLGLQDDVDQCLAVTGGSADEDERGNESDVEGPDPSSCDVQGFSFSSHWSRTPSRFAGGAGGGDSVSVSSNGAPLYRAPHGSAGFSAANATTTAAAAAAAGAGATSGFSAPERQVAAKLPADHGTAAGAISLPLPLPLPILVSADFDNDPHHPPGDPRQTTSRRLLQERNDGGGGGGGGATSLQMFDSFTPGGVPARPPSFLPQALELRTAPGCMTSSSAPPLPPKLGEAAAAATAIATATATAGGGGRMRVTDSDLLSSPSPSLSPSSPSLPQHRFSLRLLPEQVQPPQGSREGPQQTEIGIKKMAFGDALQGAPSKRSLQLVQQYGDEAIGAVGTTTLGADIEAPGKETRPGLRAAQQRMQAARANEEMLQALLGSSKRALPIASPRTADIREGGAAAELERFDRVQDAMSRLDGILAASPPPAPAAPAAAAASARSLNARVGASRAEDAASDFPGATPATSNPDYGVKVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRL1</accession>
    <name>A0ABQ5RRL1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001938</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903">
        <property type="entry name" value="GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903:SF1">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="3">
        <location>
            <begin position="1564"/>
            <end position="1707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="455"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="634"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="873"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="932"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1009"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1755"/>
            <end position="1862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1954"/>
            <end position="1989"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2090"/>
            <end position="2117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="648"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="873"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1020"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1755"/>
            <end position="1769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1793"/>
            <end position="1802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2437" mass="247255" checksum="64D969B7160E9128" modified="2025-10-08" version="1">MDKVRLKALADNLGSEAAEGHEAALHELHGMVSRATSKHNFDPTAVLETKLLQILLKHVLPEQTCDEGASALLLVRKRQLAALEMLAGDEKDEYGLLYWASARGALISVALGPDCKALTVLADVLQCLAASPTEHQPECGAVLAGTARTLVRLLLRSDGLYTAYAVNSDQVKQAITDGALPALVDAFSTYIVAAVMETSIDVIGPPPAPSALRGGGPGAGGRTLQRWLSAKQAHARTTSQRKRDKQHRQQIAAASVAHVVMQSIGYICQALTPGELRETGVLEVVLAPVVALLAGLSAKLVAAEKKAADAASAAAQATAAAAAAAMAAEKAAVKVPARAKPPALEHGNVAIRLSYIGEDKEATEMSGGMGGGPGGTAVKLPRAPPALVVSGLLARSLDGLMHCVESRSEARGDATRQLRLVVAMMSLAEGGGGGVPSLLQLIRALCASLEQQEEEQRETAVASRHGGGGSAASGPSHMGGTQLLHLPGLAHQGHAESTKMRGKLHGVRHALAPYKVLFQLQTLCQQAIASVGSIGGAAIDGVGPDNEAALTGLADELEDVSTVAVRASLMPTLLARGDLHQAVDTAGALEDLFRHLPSAGQTALAGVLAEAAAQPSAPLGLVVAVAAAAGAMTTGQAGQPQEHAAGKSGTSGPRQKKQPQAATSSHALSAQAGAAAGAGGAAGSTGAASGSAGGSAHLDFRVAVRAATFVGSLEHAHSVGGGIQPLADEATSLMGSLPAAMMVGRMRELAAHHKAQTGGSKSMVVRDNGAVSSGTTATAAAAGGSVHGGMAHTPVGLVEAVLVGLAENLQSFLSAAAELLPSSDAAEADDDSSIGHAGHVDELVGLVCRCTQRFQERLPVVLQHLQAHLQASGDANTTDAETSDASGTHGHNGGGGDDGTALGAAGAAHQGQDLLVSTACVAATRVLETCTSQLTGPPPQQQQQQQQQHLSADTALGDQTAAALRILATIIKSPRAPLHARLQACTAAAQALHKLLLTEWSATTTSVGANVGGGITTTPSGGVAATGAAASSSSAKSDSDAGSVKSDGSGRANSSSAGSGTSTGAASPAAGVGGATTTHPLLLHDATEAAQACLDALFGVLSDSAIHSAEPDLFEDEVCAGLAVSLAELRPLNPLAAALDVKHSLVVAARALKVLVKAVPHGQLTSEQETLLRCFVNAHDDGLQHSAFNLDLLFAEGAVAAMLRRLHDTPQDQPLEEEALQFFASLTDWVPSCRHRLRDLGLLKRVIRRMESPSCGGFELVLLVSICKGMSESESLHTFMKELGLARALAAKLVADDQADGAAADTLLTVGSTGGLDALLTLANLNGGTSSPDDTLCQELIVRHDISAQIAQFAAEAALSDKGLATYTFPDGTWSIYDLPSLLISCQSLTRTNVNCERLVQHGLLPTLLAVLRNSCGGTGSGEAATTATGGAAGAAAGATNTGAAAAAPAPSSSSSHARNVFRDPQLVVLAARTIFNMAQVSGLHAQLREAGVQEVLKACLKREDLRVVDAARGTLLQLGELTDTAATRALKASCADVAPPAASAAAGKADGAAGTGGIWTGPPLYDVFLSHKRTDARDFARALWNLLVSNGYTVFLDFEFKQELGSLEEMVGHCTHFLFIMTDNVFKSEWCIKELVAAVKANVNIVLLIKDGARWPDLEGNPVCDFPPPHLLRTLPPEVQPVFTRKPVVHNDEYYRAFVEALFDKLYRPPPMTPPMDPSMDPMQMATAPLDLMDPAAAAAAAAGLPLPMAPTSAHLRSAHSAHANQSAHLHHGRGPSAHLHTHGPASAHLRQSAHGHHHSAHAGAAAYLPHPPHHITSATHPGALQHLGSAGHHHHHHQGAGGGSAGNHHHLSAGAMMNPHDPHHAAAAAAAAAAAAAAFATAGHGGLANPAAAYTMIANPWPAAATAAQAAAILNASIHNHSHPTSAGSADIAAHQQQQAAAAAAAAAAAAAAAASGDPQQHQHHHHGGQPHLLGQSSPSGDAAAAAAAQTMLLPRPQLLFHQALSDPGVFSAASAADVASAAAAAGKTHGALTELQAELSNARRELVSEVTGLGRNLLSSQQELRQSLQAEVAASARLVSGEVRSLLIDAQSQVHNLKVELADVRQELITSRQQQGEIRGMLAQVLDAVGALMQRNLQLESLVAGGHGSGAGASPHAAHSHHHSHNLVAGVNGVVANNGNNGLLRVNSSGAGAARRPRLSSCLGYGNSGNDVGLINGLDLDSSPSVAMPPGIGLRSRNSVNGALSYDSSAGQLQPPGYSPRLNSILMNVSGAGSNGGGSLGSPSDNGVQMQGVVESPRISRRYLSQPLVVKDLAAVEPSVAGMAHLIQIGNGGAVGGGLVHSNSANSSGGGVQAGHSGAVTSGGVVLPPIAAAAVLHHQQHHPSPPPGAGGVVIIGGGGGGGGTATNGVGGVMGGGFQIRAANQVNGQWRKGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRX2</accession>
    <name>A0ABQ5RRX2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46277:SF3">
        <property type="entry name" value="BINDING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46277">
        <property type="entry name" value="OS03G0850700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="85"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="302" mass="34076" checksum="00F55E71A0D9B184" modified="2025-10-08" version="1">MSDPSDSELEEVRRLCGPLVDDQLKKGGEGGLTNATLRRWLVARKGDVKEAAKGLQAHAAWRVKYVPNGRVFPEEVQGELDQNKAFLPGFDKSGRPFCVVIVSRHQLKDTEMSKRHIVYALDCASLLGSTKPGWDGKMSGIFDLRGLKASNCDLATLRNVFDLLQNHYPERLHTLWLYNAPVIFYGIYKMVYPFIDPVTREKVRFVYEKDAQAEFLSAFDPQVLPPELCSRGTGRWVTIKEQYDRLLMLQQQHQLHQQRQQQAELDVVALSPAPAQHGQKQEQEPQQEVAGEVGLGPIAVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT04</accession>
    <name>A0ABQ5RT04_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002965</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF147">
        <property type="entry name" value="CGMP-DEPENDENT SERINE_THREONIN PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="137"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="448" mass="48781" checksum="833652B1A349F5B2" modified="2025-10-08" version="1">MLMDGTLISVEGPQPATGPSSLGTGESKLCLLGKDESLRRCGRSGRASRDLAAPQSPSMTTVGLVTPSIPITLQGLAAVEVRDVGDQNYEPRAVRRSLEDGAKARRAALHDGASISRTKVASAADRDLLAAAQKTDIRTVKVLGEGAFGIVDLVRVHTPSGQLLCVRKKLLKANESNNNDPAREVEALEAVRDCSFVTQLWSSVVGLYDYTLLLEYCPYGSLEGLLRVVSCHRSATGNACLDFAFASLLRLERRAGLTEDEARFYTACILIALEDMHSRGYVHRDVKPSNCMLAESRYLKLGDLGLAKHLEAGTKAHSQAGTPLYMAPEIVHGNKSGYSFSADIWSVGIMLWEMVDGALPKWAAVSWYWTKTLHCPDKFTPELKDLLGQLLDKSPRDRPTATDALCHRWFRQIDISALRAQALPAPEPTELEALLIPSRVSDAGKRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU36</accession>
    <name>A0ABQ5RU36_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Misato Segment II tubulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_002679</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the misato family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029209">
        <property type="entry name" value="DML1/Misato_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019605">
        <property type="entry name" value="Misato_II_tubulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10644">
        <property type="entry name" value="Misat_Tub_SegII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14881">
        <property type="entry name" value="Tubulin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Misato Segment II tubulin-like" evidence="5">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="DML1/Misato tubulin" evidence="6">
        <location>
            <begin position="170"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="131"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="555"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="664" mass="69447" checksum="1A11082B61FD7547" modified="2025-10-08" version="1">MPKEIVTLSFGSYASFCSAHYWNLQDEAAGYSSLEDWSGYAACIDHNVLFLETEGRNGFHVHRPRAVLFDIAGSSGGLSFSEDAVAVPPSAASVSSWRGACEVHRADPIPRSAFLERLSEHEDLDWETMGDDEAAKQQAALEAAARQLDPGGGDASRGVTSPASASSGGVTRYWTDFCKVVFHPRTVYSLPGSWTSPYEHSLAAGWGAAMDLLDGGGGRGGGGSGLVEDAVDRVRYMGEACDSLAGFQVLIDDLTGFGALAAAVLEEVVEEYSGRPLVLFSVRQPGQWERQQDGGSAGGSMSTASKPARVRDDLSQALALTRAAELAALYVPLAAPAVSGSLPYLSYNALLPYHSSAILACGVDTALLPTRLTDARSPLGEPLGATDLHSMVRLLRPGGSVSLVGLHLALPCTALPAGVQQVRQQLDERVRPPHASGAVGTTVTAAGPPGEVLRGYTAQLSLVADLASLTPGIIGRDDGRGGLGSRGASRAESYCLRGGRKDTGPVATASALEALDLLLLRQPHRMCVRHRCTAPMPLAVPLPFPKLFRSSVSQHGDVIPPKPELRSSTVAGSSGMGGTSSPSSLAAARAAGESVHSVPVLTRLQVTGEFEGWVRWVGDRWARATVGAAGRAVLESWGFGRDDAAEIGSQLDDIGAAFADEDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUJ7</accession>
    <name>A0ABQ5RUJ7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="4 8">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_003003</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="4 8"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00948">
        <property type="entry name" value="FBP_aldolase_I_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029768">
        <property type="entry name" value="Aldolase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000741">
        <property type="entry name" value="FBA_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033379">
        <property type="entry name" value="FrucBisAld_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00274">
        <property type="entry name" value="Glycolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00158">
        <property type="entry name" value="ALDOLASE_CLASS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0704">Schiff base</keyword>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="439" mass="47375" checksum="B884F5486A57A14E" modified="2025-10-08" version="1" fragment="single">THSVHLYAPILLVHIASRCVISGLSSSDKRHPNRARTVTMQAQMQFQSARTVSCVRATRGRAQPKVLCRAFNSMESPFAEELKKNAAYIAQKGKGILASDESNATTGKRLQSVGVDNTEENRRDWRELLYTAPGLGQYVSGAIMFEETLYQKARDGSQFVDILLAQGIYPGIKVDTGLQVLPGGKGETTTQGLDNLADRCKAYRKQGAKFAKWRAVIKIGEAGCPSTTAILENAHGLARYAQICQENGLVPIVEPEVTLGPGDYSIDETAFWSERVYSHVMRLLNEYGVILEGILLKPNMCLPARGHAPPGLDAPVASPEEVARVTVRTMMRSIPPAVPGIHFLSGGMSEEEATLNLQALNDEAPNAPWALTFSYGRALQATTLKTWAGKESNWAAAQDILVKLAKANSEAAAGKFKGPHPVPGGGRILQALRTGGSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV35</accession>
    <name>A0ABQ5RV35_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_003172</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="11">
        <location>
            <begin position="169"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="114"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="221"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="476"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="500"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI60936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="526" mass="55140" checksum="1A182EC5AAE6B957" modified="2025-10-08" version="1">MFKKLWKEIKKSFGNKSTSRKVEAPRLGPIQPAGDCFKPAKQMVPAAASSNEMPTSTEPAPRPAKSAGACPSYAAKSVVGQRMTMEDSWQAVPDLLVVPKWWTCNARDHLPPLVTVPAEGAGDPQPAQQQGQETKVAASTATTETAPGSSGRQDEGSSSVPEPVSKVSRSALEAKNLAEKYGTDPVTVHFFAVYDGHGGPDVAKHCAKSLHEHLKAAVAASVKPGGASDSTSPVSAPVPAAPSSTLETGGTPVAGDPPLADPWPARVDSIEAALKAAFLHTDAQLAQNRSAHEVGTTAVVSLVTARTLWIGNCGDSRALLCREHEAVALSLDHKATRADEVTRVEQAGGYVWWDRVMGELAVSRAIGDHCLRPFVIAEPEITSVLRRSEDQLLIMASDGLWDVFTNEEARALALEKFNGELQRTSSSKMAVKKAASSLAKAALAKGSRDNVTVVVVDMRTQGLSCQSSTGAHSISSAIRSSGAARTGPSQSASGLAPQGEQCQPVLQQTPQTSASPRTPAASDTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW46</accession>
    <name>A0ABQ5RW46_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003808</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="27241" checksum="BA19C440AB58F095" modified="2025-10-08" version="1" fragment="single">ASYNGVGRNDGKLSITRPASASASRGCCIPGQSPSIAPAGNLRNRPASANSVGGARRASAGSAAAVAVFTQCGGGQNVSEPRHRGPAAAVERMGLGPLEEDVGYEVAASDMLLAAGVGDAGGGSGALGRGPCEGGEGAAPMPLQHVPPTQHMLLSGSLQDLCERLARPGDRHLHLSLGSVSFSGDLDPDTGNMIQIQNRTVILHNCTLAFKPGQKLYVGPGGHVILADVIVVALAGAAAIAEGNGNGSGGGKMAPTPHGVGRPPPPAHHHGNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWA6</accession>
    <name>A0ABQ5RWA6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004427</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046891777" evidence="2">
        <location>
            <begin position="35"/>
            <end position="2193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="929"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="393"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="446"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="591"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="932"/>
            <end position="970"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2193" mass="237536" checksum="8FC7CA7D42DD2C2E" modified="2025-10-08" version="1">MGLLRRPGLIMALPLKSRLLQVLVLGLIIGASHAKAEGQTCWKALTGTYNALVVAATENVRMREIVPPPSAAASGNNGSAEPVVSIIPVSSQGSFMYLRPTYKLASCDNVTGSSQLTVRIVVQDCDTFAAVTTPLSPADCTEDNRCTRNASACSSPCATGLMATLEGSFIVDASGTVSGLSLRVVPDAAVSFLLGAGGAATTNTGSIQGTAESLQGDLTYLMETAVACRDGMSYSGPSSSTEESGALLESVYGLFRLLSDQAHIRMDNVSEEIPVLSYGGMGGSNGADDGSPPPSPPPPGSPAGDKYERVETPGFYVQNAETKVTPASDDGSQRVTTVRNVVPTDAAHEAFQGNPYSIPAGGMSHTLISESGKVVARVDMTLTELVLPPREQNGTFNNSSDSSSGGEGAGVSGNRNGTFKEAPVIRSRSILLMTQLPRAPPPKSSSPPNVSGKSSIGSSAASLRTSNSMVNTMDTAYGLTSGSKTTNPFSAGSSDPIPQLSQQLTLDIEVLKGLSSLNVDDVYPGGNGAAAALKEHQAVLAERARRRRRRALGILQAIGADPQTLSELAAEEGVEWPVASTYDGASESDTDISPSTTRQSGSLKRSRRRKMQNLDLNIVIDFSKYSSTKVGGYEFGVMVGAGADAALVRKYRNVTAGAGVYSYLHVNLFNRWFNDSVLFKYGGAFSVTRYPNGTYNTQNSYFRKLFKIIIFKIWDETNPALLTTKNSCNSYKGLMDKGFILANSWSLGSSKANQSFDLGEFTKVGIRLGILDLVVSLDIGVDLYLAMGTCNYQNSNPSVGAGAGLGGYVAVAAEVSLNIIIFKATLRASVTLVRPVITGFAFWTYNMLMYGERWEPSCYTVDLELWGFDIHISFTIKIGVWIFSYTKTFNIIDTDGFRFLTWSPFGTGFKCPTYNNAYNIPYTDKLALADVTPPPPPRPPAPAPPPSPPPRPPPPPSPSPPPPPRPPFAPSEPSAPSLAPAILLFVAEWTDATPDAQGMVIEDDNDFVDVDLTVSWLVGKTVYSIDVETYDDAETFSPSTSSARPPPPPIQTVIRGGVHGDDNTITGFNNESVFWAGRYGNDRPDDSIYNICLTLYGDARNKTGLVLNVTISVYNEGILVKQYRRTWDTSTSKGLPPGDVCNSTSGGFMGNYDFNPRFDPNLPAYEPLPDYIRPEVANFDAFTGIVSSTSSSSRRFVRAWLDADGAYSKRGGPPALLNLTGNCLLYQDTSTVAIAFDGTTCSSLSSSSLPKSWNTSDGGPFTAVWIGSFTPNIDGTQTLLSIGRTKNDFFWTSQYRSSQSWRSDGNGFFITSSDVPEPPEGQWTMEVMVRRGRGTGISCHRYNKQSPYLQSWYFGSQPSEIYPDAFAVGSDPWISSERLLNGRVSVVLIYNRALPSNDLLALASFFAKRFAWAKPLGDLPAFTQLPDYLRPEVANFDALTRIVPAFTSGNSTSTLIPLKAWLDSDGAYSKRGGLPAIINFTGNCIPYNDGLTAAITFDGATCTGLSSRDLPSSWDESAGGPFTAVWIGRFNPNVIEKEQVLIMTLARAKTELEQQLYWGSTRRFINSTEVKFGFDIRSSDVPSAPAGLWTMEVLARPLGAESVSYYRYNRLSPYLQTWNFPSKPMEIFSHVLAVGYDTRFPGRYLDGQVSVVLLYNRALSENNLMDLASFYGKRFAWSKPAGARRGYRRLPDNLLPEVVNFDAQIGIVKSDRDVAQADASSTSPSTSVVRVWLDADGDNSKRGGLPVQLNFTGNCSMYHDAFTDAIAFDGDTCAGYGSRDLPSSWDKSGEAAFTAVWIGSFNPNVSKLQMLMTLSRSKADFNEELYWESTYRFAYAPVSNASGYGFELDTSNVPAAPTDMWTLEAIVRPSGAASVSYHRYNKQSPYWQSWSFSGKPSKIFPDSFAIGADIRDDDGKFLNGKVSVILIYNRELSTSELVRLSSFYAKRFSWPEPMYAPPPPPTKDCQEFPGYIASPKADHYGDDIIRYASLTMAAKQCLVSSLCKGFNTDGWVKTKAAPLAFDPSRCFYTKVEPPPPPTKDCQEFPGYIASPKADHYGDDIYKYPILIMAAKQCLLSPECKGFNTDGWMKTKAAPLAFDPSRCFYTKVPSPPSPPRPPLPPPPTKDCQEFSGFIASPKADHNGDDIAKYSSAATAAQECLKLPRCRGFNSNGWIKTTATPLASAPTCFYTKIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWM9</accession>
    <name>A0ABQ5RWM9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_003873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046304491" evidence="2">
        <location>
            <begin position="33"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="296" mass="31271" checksum="3B8567EC02603F8A" modified="2025-10-08" version="1">MESLSMRRGHPLQRRIATALLLVVAATHLLQGQPVALAVGPSSRTSKGPARHSAGSPGVIPPLFKSNPFKGSSLLDALKHLRLERGDVTGAARVDTLRKWFSILGLYRGLLPLSMDLFKNRGLAADIAKDWTTMVERLAVKPVRGAGLAPLRPGSATSTGQLPELLAANPAEVLNWVRATATKAQKAFKGSAVSRLLADLYHELSADPFFITDLIKAAQLVGWEISATATLHVGSGSGSGGSWLPEELTSLAQQVLDVLREELFPDATPHRSGGGSKPSRQGPIPGKGLGGRGTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX79</accession>
    <name>A0ABQ5RX79_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="57"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="170" mass="18916" checksum="F6E44E40E4409379" modified="2025-10-08" version="1">MAFRSLDTLCFRSAPSTRYSRCPVIKNTFAPLRNVSLRHNSGLACKAMMAQEAAAIIQRQLELYNARQVEHFMDLFTDDVVVLDGITGAVIASSKEELRPRYVERFKSPVHCELLGRLVLGNVVVDREIITGLPNGGVADCMATYICDVAAGKIKRITFVWQPRTDGVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX89</accession>
    <name>A0ABQ5RX89_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004791</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="231"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="341" mass="36918" checksum="AB2307703B4EB0C9" modified="2025-10-08" version="1" fragment="single">REPRLILRSSRRVPLLLAYYALQPLTPFWYHYCAPSLVSFWSFYCASLESHPLLTKITTGIVGTILGDYIAQKLSHQNQVRDAQLHGKPAPSFVFDVVRTSRLVIYGALVGTPVGHAWFQVLDTHIMPEAMKSAQAVLTKMSMDQLLMCPATTALFFLVMRCWEGHPNDAVAYMRGKIWPTLKANYVLWPLAHIINFAFVPPSQRILYCNAVGVIWTIILSTIWSEKTPPAPSSISIQSGNRGPGPAAGSGTGPLPGGSWGGSQPAWGAGMNTEQSSVPQHFHGAFTPGIASGATDVSGTSCGFGGDQQQPLSGGKGVVQPKILAALFMRSAHWDTKSKDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXC9</accession>
    <name>A0ABQ5RXC9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MINDY deubiquitinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004796</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007518">
        <property type="entry name" value="MINDY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033979">
        <property type="entry name" value="MINDY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063">
        <property type="entry name" value="NF-E2 INDUCIBLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04424">
        <property type="entry name" value="MINDY_DUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MINDY deubiquitinase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="MINDY deubiquitinase" evidence="2">
        <location>
            <begin position="539"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="482"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="793"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="797"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="807"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="874" mass="93719" checksum="B1D98A069BB2EABC" modified="2025-10-08" version="1">MAETEYKIKRIQFFGRISTPIFMQNENGPCPLLALANVLSLRNQIAEIPTSVTSITEKRLIAMVAERLVDSNLGSAAAASSSAYAADLQQNLSDSLDSLHKLAVGMDVNVRFNSVFGFEPTQYVSIFDLLDISLCHGWLVDTDDMETMRVVGSRSYNELSEMIVATYDAAQQQQQQQQTQEAGGASLPPLKSFSPPAAPSLEFQGMDGQATTAEEGGEMIAAVPGRQVDASTWQSGEGTPPRAATAAADGTCQELDGWAAVSASEAVAIGGASIFAATSTLSSAGAVAEALVAGPSQPPSLDQPLELQTTSEPCRQHPQELATEPQQQTIEQVQARPQPGQESFKQMLQGQVDLQPHQPEQEENELVTVVRATQASGTGHSLTGVTENGATSAACHLAATPSPASPALAPPSSLEPALLQLAVPPPQQRDLITFDDDDDDDAIAAPTMASAPEAAVNSNQAEPVIDLADLLDGPLLLQPAHVGFQELPPPPPPPPPPAPSPLPPPLPTDPPAATTIALPAATAVASLTHPAAANTSDSDAEVLARRVYDAMVARDFLESTCSQLTVAGLRALQSSLRPGQLAVFFRNNHFSVVFKHGTTHQVYLLVTDQGYLNEADVVWEHLSSVAGDTQFCGADFAPFKPHREPVEGGPVAVTADVVMSEVDAAAIAAMLAEDEAQMRGQDHDAEMLDMTGLGLNIPPAGQPAVAAGAAAAPVALPLSTQQQQQQQQQLQEWQQQQQQRRQAQHLEWDWQQQQQPQTQLYPPLVSTRQQQQQQQQQHDLDADLALALRLQQEEEEEARRREEQLRRQQSQRPSQPARPSSNTPPTGRTHHGGTFTPTYRQQQQQQQQQQEQQRSSAGRDGQSAAGEKEKCIVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXD2</accession>
    <name>A0ABQ5RXD2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004648</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF31">
        <property type="entry name" value="OS01G0873500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="403"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI62114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="427" mass="45725" checksum="091BD88C4F4DF0FD" modified="2025-10-08" version="1">MAWDGLAAIFSANNHARRSQLRQEYTKLAMKKGEALPVYLSRVRELHTDLLKVGQAISEQDMAYAMLTGLPQKYEVMMSVLTTQSGELTLSSVTAQLLAFKARVGSGHDASTSSSAFVSAGRGSSTTGSAPRRGGSHVRGGSREPPQCWNCGQPGHLERGCLAPRMAQRTGSWSSAPIGPRYGSGGLERARMGGHGEAAGGFSSGSDRQGYGRHNHVEGAGGRSNASPKNFAFVASESDRMKGWYLDSGASHHLTYDAEQLIDIRELAESEKFEILSVNGGIIRPTAVGTLRLVSMVTMAALTVHEVYLVPERFANVLSVAALDQKGGSVCFENGEVTLLKDGEKILTGKRCENLYALACISIPRSAFRSMAEQPFGGVAHRKSASESMDLWHRRLHTYQRGGEGQGGNYGESTGDIQWLSGQGGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXH4</accession>
    <name>A0ABQ5RXH4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_004822</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12413">
        <property type="entry name" value="RRM1_RBM28_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12414">
        <property type="entry name" value="RRM2_RBM28_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051945">
        <property type="entry name" value="RRM_MRD1_RNA_proc_ribogen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="11"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="354"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="95"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI62225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="437" mass="47015" checksum="0E4D73F8534ECA6B" modified="2025-10-08" version="1" fragment="single">MAALKAAKDLRTVFIRGISFDANEKDIEELFSDVGPVKQCFLVRVKDQPKHRGFGFVQYALPEDAERAITECSGKSLKGRKLQVELADKRASLEERKKKRKLGQDGDGDGVADDTANTTAPAPVSQPPLKASGPKVNAGAAKKIGGENAKAADSAKPDDDGVAAAAAALPPRKRQRAAPAAGVTAAATAASEKHKLLRAVAIGNLTPASISLAVSLARKVAPAEEVLNPAPPEVVQQAKLEADGCSGNVVIVIYKTVKDAMHAVTQLHNKTLELRKPNLKQQHHQGPRGKKGRKGTSHQQQQQQEEEAPEGREAVGGEASDQQQQQQEEEEAAAPRITLWAREVKGEGAHVKQWRIILRNLPFKVTEAALLRVLSPAGFVWDLKLPRNPDGRLKGFAFATFTCRAHADKAIATTNGKDLMGRMVVADWAVSKTQYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXU1</accession>
    <name>A0ABQ5RXU1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_004674</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF48">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="723"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="820"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="3138"/>
            <end position="3161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="3182"/>
            <end position="3199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="3269"/>
            <end position="3286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="3298"/>
            <end position="3315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="3370"/>
            <end position="3392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="3404"/>
            <end position="3424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="3444"/>
            <end position="3464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="3476"/>
            <end position="3498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="3518"/>
            <end position="3539"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="12">
        <location>
            <begin position="365"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1004"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1189"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1301"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1410"/>
            <end position="1735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2052"/>
            <end position="2234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2252"/>
            <end position="2275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1014"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1034"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1051"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1072"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1200"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1311"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1337"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1351"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1371"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1429"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1476"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1609"/>
            <end position="1619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1690"/>
            <end position="1699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="2122"/>
            <end position="2133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="2170"/>
            <end position="2180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="2190"/>
            <end position="2224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="2255"/>
            <end position="2266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI62093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3613" mass="392247" checksum="B9B6EE7DB10894E1" modified="2025-10-08" version="1">MPVHFASDSPPPNGGGGGDAQGSVWGNLRLMLGQPSFSDQQRGHIQQQEMLQQPVELQLGVAQQKKAAGFLRPFSASRGNEVLRLGQEREEEEVLPAPGAKDWKTFVAGWLADEEEAPASVDRPNGTVTMEMNSALAELARDGPSSVGAPTQAPTAAAFAVQKSAIKKPIMRGVSAAPKGRAKSAMRRPGAASARQHVIVEDEGYKEEEEDGALPAEMGIMKKKRKKKERDRKNPFVMPALHTFAFENLVHNIVWRVGRNFGFQAFNVNPRTRNRGHNAEWTPATIFVAGDHLTQLLVKNYFIRRDKGEDERQEERFAKAVFELHTTIFYSYDEYWTHMHGLSIRPMAMQEVLSKHRFCDEAVVCGLLTELALYFLIYTESSSMRHTPELLWFLYWCMNHSYVMQDMWGRDPPENFPNIREVRIGLRNKHQALIRELQHTLSIYPDRIRPEDCGRLAPIMSRLKASDVSEDQRIIVADLIAFGDGNFYYERIVTPIFYVISYEIDHLSNLGVEVAHRLGYDDINESMAHTGVVRLALRDMRVRGEQIIRGEVNEAYNTITRLGYTRGRAETNFDPQVAADWWRSNVFVKTYRERRTWLAVFRAYYRVYTWHLVLYHAMQAQAFVGWGKWENWRIISSCIITHAFCTALERFSNWYMTRNPAEPLQTALSKVFDKKGNFKISRRANAQALAAARAIGFGESKAIGSENVRMEPVRVVEIEGSPYLGTLGLVEWIILAFFILGWYVMQFYKGPYQPFCRNNWKWLAGCYTGAYGLHFLLTTRDGYTISLTHALNLGPIFKASSSRPDPANWIMGTMHMKWKLFFLDALFWIIAFAMKIPFDYYIICKPSVEPLKLTFKVNWLECSDKKYFKKIPCIGGDWVLAFVRLAPFIIVVLLDTSLFYQVATTLFGLFRGLFKLDLGVLTAWDEVVKEFYKAPYRWWFKCMSEVGNRNQLTLLKTMLFDNAADPETGAIVSDGRMFKKAVLTPEEIMGTKKRSVDVAAVLDGDDDDDNVVSGGAGQGPRGGPGAKAAAGKDANGGKGTAAGGKGADNKPLSSGWKTALQNFGLRSDHHQQQQQQKLQPKTGPKGGPLLPTTTNMKAMQKQLNQMQRLPVKLPMTTITERMLANTERQREGADAASGNKPKLASVAEKLLGNKTGGGAAAAAAKLKDLSPEEKRKEIISRIQAAQKLRKGGGAGGGGDAPSAAGLPAGDLMTGSAGGGGTAAGMLINAPPSPQRAGPQMRWGRAMAALAPAGGSGASGEPRRDLLTMDSKVSDEPTKAPDEEAALRPFSAGTVGADSRKVLLPPAGRTASSVSSDGEGVGSSGAVKAGASLPMPVEEVDDELDDAEDKPASAGSAATPATLERAGMLNPAINSTQNRASTPTLFGGAGRMGGSGADGAVGSGSRLDGIGAIGSQNRNRDRMVLDSLLPAPATQPAPAQDDGNSFGFDTGGSGRRDLPQNWPSLQGGSAGSPGSARMSTSGVPQQPSQTPGASTVSSMDAPARFSLTGAPSDKEAMARTPSSNRQAAEAARVSLSPGGAGGGSMPRPSRLRNKGKTESADMGEYQPVASPRLGTPTGASQVDDVDDGDLNESYQGYNGVFGEQGGAPTGGAAAPAPSSPERGTTPTMPRPVRISPRSPKRSLGVDVEGSGGGSADLLRVASLGPTEAHSGAAQRIDSPPTSKPGSGGAAPAGAAGSGSPFSPPGSGGGRASGPDGSMTRFSSNGSGPRPGPVAESLADRIGDGLEAAAGNWAALMQSGVGPLMSRADRLMAWHEDEDAEAASWWTRRLEAAKARSYRMRTSAINDDREVAVLSSMTAFRPDGDPLAMVYEWRVEDAGAIAAAAADGDAGSARSSTDGDERWVKGAAGVAEAREAGGDGQAEIDEHLTPRLGARTAAELPALRSVASCSGADAAAGAGSSGTYASESGGDCVPPLGWAEVHDASRRAGPIAEGTGKGSSIRLGSPDLLGTPLRYNPPAAEADVDLGEALVLAKKLSPEDGGKEDDAGGMVVRSVNGTSVHSNVFKDIKDDDAAERVRPAPVKLKSRLKLEPINFSDNGAPAASPGNGVARGDGDGESGHGSRVAGGKSGRRRSVAFGGQEVEEYTVPSSSERAESPSVALVGSGSNRMTSSTGQDRPMTAADGEGGIMSRWLAGLPGWTGLNGNKVSPAGAEDDEEAADEEAAVRAARQLQGGGGAASTGQGSKAGSGQGSKAGNGQGSKAGSGQGSKQVGGRSFRASFRLAAPSFRAGIASFRANGGGAQPTGAGQGRPTTSLPRSVQARSQAIGPPGLRPGSAMRPLTALRPTTAMRPTTARPMTALLGHDPAKEAKRKEKKDREQAIERAAGTLTRSDSLVIIDGADPDEDEEIDEVHAQMMMWNAFAFAWDEIIDDLRQADYVNDKEVSMLKFVRLDMGGRGHGLRPILLPTFFYAGQVRKVVDTGQVSTAQIMVLNELRVLVVWLGCQVGLLSGKHAHVITSAPFVRGNINVKHALLRKKMLTHGLKLVDQLEQICERREVPFDMKEIADNLHHLWVSLEGECFAIHKAAERNRATAEDVELASIMFEVVSDMKQVISSDPEGLKAVMKTALLNNATADYKELLRVIRVIKRMLVTTEAEATPQSEESQRILGFFINSLGHPSLDKPPSIDKMWSWSIMTPLYEEDVLYALDAKALSKELGLKNKKMTDLLSETDDSISLMSYLKAMFPYEWSNFKERMKSLNPDVNVMDLSEHDFAPGCEMHEFKLELQMWASLRGQLLARTVHGMMLNEVSLRVLAKLEHPMPPSMTEVEYKRYIDQLVNCKFEYVVTPQTYGKNRASKDLRLRWLASSIDILMQKYPRLKVAFLDHAETDNGPLQFSVMARGRDLNDPAQLALLTATGIQEDENGVIEWYRVRLPLNKYSGRGVIIGEGKPENQNHAVIFAFGEGLQAIDMNQDNVLAETLKSRNLVQELLPSTKGVFHLFADDDEEVQITRKTIAAELLYVMRMRQAACTFTALVGFREWIFSDKAGALGRFAAATEYAFGTITQRTLTHPARIRLHYGHPDLFNKMFVMTRGGISKATRQLHLTEDVFCGCNHTLRGGRIRYKEYISCGKGRDMGFDSINGFNFKIAGGGGEWAVSRESSRLGARLDFFRLLMFYHSCIGFYINSWLTTQGAFWNIYALLVFNMAKASHMSDMLQRIYNVQQILQLGTLAMIPYIGQLILEMGVVKAVVTVFQQILTGSLFFYMFQQQTVASSFIADMTYGSAKYVGTGRGFNITALDFVKIYTLYARSHLYYAFELLSMLIAMYVVRGCEVCNYGSLTWSGWVLAFVLIFAPLWFNPFSFDIAKVKVNYLAWERWMHGDVDPGTGTNWYTWNSGMLEKMRNDNGNNTDNWVNIAFVGVGCLPYILLTVCAASRLRIVAPSAAKVLHSQVMVFLIATVSIWIFVHVSIQIKTYFTELADHKPYRIYRYMVTICMVIFLILWLALVSRWYDGNGFNTLCIILYANFQLLVAYHKFVTVALSQNNTMRAFVDSFYYTVDQVIGYTLFLLIAFLSFLGVVGALQMKILFNDAFAQTAGHARIARAMKDNKVGFDMRGKAPGKPGGGGKPFGAGGAAGSGAAGGLQARSLVTSEHKYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYJ6</accession>
    <name>A0ABQ5RYJ6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33675:SF1">
        <property type="entry name" value="HOLOCARBOXYLASE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33675">
        <property type="entry name" value="NUCLEAR RECEPTOR FAMILY 2 GROUP C PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="216" mass="22840" checksum="E307E19CC264CFB8" modified="2025-10-08" version="1" fragment="single">TAESSHHSVAASVCSVREAVTSGVNMSLRCGKKRKLVVDHEAEKSIYGSFVSAANAVSTLYSQAVQQQRRASAQASRQALERVVTFLISQNPGSEMVSKAVLLNFLQQEYESIEGGEHLPHVFPAPQLSVAGPGPIVGELQSDHPGCSKSMRGISVGYYGASGVSPARRQHSGGVDGMDSSDNPMQQLADPQPPAQQELHGFCPIPSGQAFPPQMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYQ1</accession>
    <name>A0ABQ5RYQ1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="255" mass="25971" checksum="B3B9C7FB8C566D73" modified="2025-10-08" version="1" fragment="single">MIWRMEKERRGALLIGLSLPAPIMRILANRPLAVLEDSDGEEEDVKRRGEENVDGEDGGGGGSVRSGGSGSRSGGGSGSGSGSGSAGGHTDRALSAGGAPGGTKMTYGRQDAVSSAVTSGGGLQRIPSERVAGVEELLGKFGIGGGGGDGGSGNGRSTGSMTAAKSNGLLIQTQGWGVRVNGKLLGPSAANLLKFMIPLALWQVALGGILSVSYSDLHRMQQPLVILNMASRVIYRYSRLRMVALLLVAAALPTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYR5</accession>
    <name>A0ABQ5RYR5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_005504</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045867146" description="Protein kinase domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="455"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="341"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="540"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="680"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="341"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="553"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI62762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="915" mass="96887" checksum="E4BED464E49EF8BB" modified="2025-10-08" version="1">MNLFLLFMVLALCNSISSASLSETSLGSSEAVVYTDKQLFQAICSQNTVIWIRADLDLREENWPKDCNAPVVLMNNMTISGPLDAPLYYTVSFNFMARKVVIKPYGEVRLRHFTARNTRRGGGGEIDFIAFSPQGILWTDDLNKNQIICLHSDPSKLIVLPLPVNFTEFANKSQSVKLWDSYCTQRQCWNEVLHYDQFFTDVNVGSDSETGGYVMAKNNASKTCDQFVTNECLQAKGGDACMAAVINDFLSNLPAVDEGGVNRLAIVLPAALGGSILLVAAVAGIAFLAYRRRQQRRAQQSAAATAPEQLWGKEVELEFSGGAAGGTDSLPTEVAYLPTGSNDVWSRSSKDTVSGSKNTEDPDAVGGQVAVVFRPESGTVGVRAGGQDRCPDAYGRGTSNSFRFETEDDMGRCLLGPRRNALGLSGSVHPSSGLSGGFGAALVEGIMDDGSVCTISVGPPLGQGSYGIVFKGTWQDKPVAVKLILCEHNCSEQVATEVRLMTSAACHHPNLLRAFTYTTRAKVLTKQPAGQALQSTISSLMNSSSRRRDPHTNPGTDQQMMGASGADSGNGSKPELAKTWIVSEYCDLGALAPHVHGAKRFLFPPDTITTAAPGPASGAVGEGAAGLGLLRRPRMSLILSVLRDIAAGMKHLHALNIVHGDLKMANVLMSSRQPEVVGKAIGSSSAGSGAGPCQGIPGSNSEPGSGAVPDAANPSLNISGKLAQLPPEMIAKVADFGLSRCLQEGQTHHSTKTVGTVTHMPPELLRSGKLTLGGDVYSFGIVMWELLTGSVPYRGLMYGEVVERVVVSHRRPEFPGHTPHEYRSLAERCWAADSAARPTFASVLAELEAMLAAAARLQAESDMLYAAMLLPSTATATAGAAAVDNSATPGDSAVATVRDSAAISASSFTTHGLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ29</accession>
    <name>A0ABQ5RZ29_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Retinoblastoma-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MAT3</name>
        <name evidence="12" type="ORF">VaNZ11_005653</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the retinoblastoma protein (RB) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024599">
        <property type="entry name" value="RB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF17">
        <property type="entry name" value="RE32990P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11934">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01367">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01368">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein N-terminal" evidence="10">
        <location>
            <begin position="78"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="11">
        <location>
            <begin position="429"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="727"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="967"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI62882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1116" mass="120328" checksum="105548DCCF0BAFA0" modified="2025-10-08" version="1">MDAFTSADAPVERLSCLFKDVKINDATTHEGLLKLYEELKECYDAQETAAPTECMGEQDEELKQICTQASLLFVARVLFAAQSRDDRIVPGNLGCPISRIVAAADINLLDFFREVNVVVSKLSAFFESRGGSSKLFTQQAQLKENSETVVVMGLLAKKYKDNFHIFMHQLDFYKQLVLKLGWSAFLVLRVKLLSAFPDVVSCMELLPCVFAVLACHAPRLPDCLSHLSHEDGRPALLKAMSEMCKADYSRVQARMPSVVSLLSQILITAVPEWTAAISGAKIQAKQIGANVAANIAFTGPLDLMSCPVLEGLVTDADRMQRALTALEAEYEQHYKHGGSELDEREFLYTDFTKFASPRFSPRHMHSAITKLQSGPTPLRQGALLGPGAHTTAPSVVSSHLVHLPMPIPTGMHSPLPMFNVTMDLGQPTTPVSEIMSASAWLRGMAANLATEPSPGLVRYLDAVGMPLCGTRSTCMSGHVSAAQQLALRVRDLVNSIMPEEDNPSLLGSFPLLQSSLTVERRTEITKLYYHSLESILQTEENASGLAAVASLLASGKFHRALVACCIEVVAACYRMVSCAFPKVLDALHIKAFDLAKMVQSFVKSIETLPRELKRHMFLIEEKILESLAWEPGSSLYSHIIISTNDGMVMEQTAATEVAASRTVSEVGLVTSVGSSAAACIPFDGGSLDVLKDAGGEVTHLKNSASDAIGLSLSSFPVSAITVGHQLSPKRHHGSSAMDLMSPSKKARGVHGSPLPVLGTAGKLPKFIGRMASGAAGIGGSAGALHDFCRKVLKLAAFRLALMCDNFDFTPLDRADVNAKVYETIEHALYYQTHLFYNRHIDQIILSALYGYCKVHKLTQVSFREIIAHYRKQPQAQQCIFRSVIIEQSNPGLQVATRADIIAFYNHVFVPSMKSFLLKGEPNCSVTSGDEVEKIAGDGNGIKTGPAACTASGGSREPLPLLPFHNTSRLPKLASPPASSSPARGLSRQAAREEAVIGASQDHEFCGAVPAGSVKDATGPRPSPVLSHSATCGLESGYGSSKHAKSTAVEIPDGLAALLQALDSQQGLHGNRVEDPQGSEVCLHWGTGRILGGEDTNVRDLGGVKVKKHSVDKRSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S046</accession>
    <name>A0ABQ5S046_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="26858" checksum="3B71FD4904F9211E" modified="2025-10-08" version="1">MKALLPCVLGHIERVIARRITLDGRTGLLCFDSLCNLFTPRRPLSSIDLSNHVVLFIPSSLREMSEQLAVYRRCRRQYPGTAACVLLPAFCQHKAEASLRGMRCVHRFEPGTVLFNGVDREGMLAPWPGTSYSVDVWYEAPDEGAVGYGRALSALTLFAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVAEVNALGLTPESSDCPQVTLADGVKHPIVGRITLSLSIGPLRVVTRPYVLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S065</accession>
    <name>A0ABQ5S065_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04692">
        <property type="entry name" value="NUDIX_Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF20">
        <property type="entry name" value="NUDIX HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="46"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="229" mass="25992" checksum="BBAE4095C4554184" modified="2025-10-08" version="1">MAQASVLGARNAGVAAEAEDEVFETFNEDGTPLGRELRSVCHAHGIWHRAVYAYLFNAHGQLLIQRRSLNKKVAPGQWDLSVAEHLSPGESFREGVVRGLSEELGVELSPQQLVSLQGPMTPMHQRRLLIPEKGIKIHSSTPDIGIIRSVYGSVLEPSTRSFKQDFEFIEAYRLNGYEGPISFNEQEVSECKWVSLEELQREMSDRPDEFTIWFREEIQSLDFFIKRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0C9</accession>
    <name>A0ABQ5S0C9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Repressor of RNA polymerase III transcription</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.50">
        <property type="entry name" value="Repressor of RNA polymerase III transcription Maf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015257">
        <property type="entry name" value="Maf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038564">
        <property type="entry name" value="Maf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22504">
        <property type="entry name" value="REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22504:SF0">
        <property type="entry name" value="REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09174">
        <property type="entry name" value="Maf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037240">
        <property type="entry name" value="RNA_polIII_Trep_MAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR037240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="235" mass="26541" checksum="9E10ED461C6AE5FE" modified="2025-10-08" version="1">MKFLDLPPLQRLNAFLKNVDVGDHILQGDLEAYSCKLAGLDKKLSKSLDHEVQTGSSPLELSASPVGPLAESSSRKTLIYLILTLNHIYPDYDFSLLRAQHFQKEHGISKAEEAIDSHLLEVSKVWAQTPGLGESPFLECLWSSIDEAINLTECDVYCYKSDLETDPFGEQASVWSFNYFFYNKKMKRILYFSCRAISKQAADEATESSSKYDKYNSDTDGDESDRYGMANEMDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0F0</accession>
    <name>A0ABQ5S0F0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Splicing factor 3a, subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SPL2</name>
        <name evidence="12" type="ORF">VaNZ11_005726</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3A2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052092">
        <property type="entry name" value="SF3A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031781">
        <property type="entry name" value="SF3A2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205:SF0">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16835">
        <property type="entry name" value="SF3A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01050">
        <property type="entry name" value="CactinC_cactus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="11">
        <location>
            <begin position="56"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="249"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="261"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI62899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="312" mass="34401" checksum="01B40663A7A999CE" modified="2025-10-08" version="1">MSFISAREHGAKPGSGGPASTQNEAIDRRERLRRLALETIDLSKDPYFMRNHLGQYECRLCLTLHTNEGNYLAHTQGKRHQQNLAKRAAREAAEKAAAPAPQKRAPIRRTVKIGRPGYRVTKQFDPNTQQRSLLFQIEYPEVEEHAKPRHRFMSAYEQRIETADKAFQYLIFAAEPYENISFKIPNTEVDRVEGKMFTHWDPDNKVFSLQFYFARPREHAMPGMPPQLPTSAGAPMPLPVVIPPPPVVSSQHGALALTGGPAPPPGLPPPPPPGSMGILSPAGLPQTAPPLGFTSPPGGGQVFPAPPPPFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0H2</accession>
    <name>A0ABQ5S0H2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MT2215</name>
        <name evidence="2" type="ORF">VaNZ11_005753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="537"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="543"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="556" mass="58860" checksum="C1CAF335E628E254" modified="2025-10-08" version="1">MRYRRRRRRCYHTILNGANHYEQKVRNFLSAMYHSIGLRSVEDYLQVEYPPCTCKVEQPNGQVLGALHPVNAKLLREFWTLKEAKLPPGSTVPQEGLFNTVHEQGYWGRAPLHSSRLSRSVSAQEFYTEAVERCVRAGRLSGKGDGRVRHGRQSVAAALFTGAQPPPGPPSSLSFPQIIKSDSSGATTNACSENLPVTSCHTREGEGECKQHGGRYEAMDRRVRGGRLLRSQSKDPHFASFSSRVMSILDTAPAADPPISLNTLTTTASYSPADDHDQPHTHDQRAEEHVMNGESRDSSRAAGQPLGMSLSRLYSLDSAALHQLDRALFGACEQSPGAASLTSPVAMEPAPASAPSGLGGLIYKVRPLGAAASSLSLHSACPLGSPFSRCNSTLLDFAEEQASLAAAQQGLCNQMEYLTLLSARNSLGGGCGGGGNAASYQSSAAGSLNASPNPHQPHVSLAAMLSAAAASNGGNRVSSGGNSGGGRSLQMQSSLPADVGRRGGPALAAPDMRFFPFSSPEDFYMAATVLLDAKLPGVLPEEGSSSPPKKGAPPCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0M5</accession>
    <name>A0ABQ5S0M5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar import/degradation Vid27 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055559">
        <property type="entry name" value="CYPRO4_DUF7135"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23581">
        <property type="entry name" value="DUF7135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08553">
        <property type="entry name" value="VID27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7135" evidence="2">
        <location>
            <begin position="10"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="1">
        <location>
            <begin position="266"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="588" mass="65216" checksum="CE5B20AB69FFA343" modified="2025-10-08" version="1">MGQNQSTVDALDAVNQVDTALKTTPQKEADGGVKLYKYVITSSGDGNWELASSNARPRIYDSKEDSNIRGKKDFFLEIEQGDVDVHVDANLNHILDVRQRRVTFAANDTIYALKFPNDDTCRTFGVDLNDALFYNQYGVDNDEDGRSKIMEDYANTVFAKDNTDRLFEPMETDDGEENAERYFTPEKLSEKQAKETAKDEDAIHSIIMGAGENNYLLRGCKFDVLRNVDGGGIEDKNISFTLTPPPGAACATPPVSMRRTRAAASAVQAVQTPGMDVTPSRVLLTHSERRMNLLTPDNPNVLHHADIEVGKVVSTFAFQKDRVDVPIVEIATDYKAAQTEEHSQFLGLDQNRICRWDLRDKRGVVQESTAVLDYVAGKDYSRGTNFTCMATSGDGFVAVGSRDGRVRLYNSRVLTQAKTSIPGLGKPITAIDVTYDGKWVLATTDTYLMLIKATYVNDKGKDANAFESRMGGRGAVPRLLRLKPEDSIKTGGAKFAKGRFTWITEGGHSERWVAAGCGRYSVMWNFSKIRTASTESIGYGGLPTCMDYSLRSKDEEVVDVHFVHQKYMRDEEQAAMLVATPHSLFNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0U6</accession>
    <name>A0ABQ5S0U6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_006123</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF35">
        <property type="entry name" value="PROTEIN O-GLUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045750666" description="Glycosyl transferase CAP10 domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="4">
        <location>
            <begin position="146"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="418" mass="48405" checksum="95DACA677746642C" modified="2025-10-08" version="1">MMIQENLIVIVLLLVTTSRALAILHTELFNSSTIQDPLVTIRHGCPGVQRATTEMDLEQLLRDNLEPDLRPWRQLGRALTRMDFLNFLAEINTSWLEYRRNFLAIVVIRNNVMRWWAYPNARVWCTRRVKFIAEQFHSFIASSGLRFPDVVYVMNAYDKPLCERGNCSAPLFTFDKRWNASAYAAGLPGSAHDDILHPVMNHPFEQLVDYPWEQKIEKGFMRVGIYGAMAFNCSRVLINKVAQTAAGREVLDVGIYQNKHWRVKVKTVSPLPMELHARWKYLINTDGQSASWRLAKLLAINSAILKYRSDAIEYYYRSLREGDNFISFDQTDVVEVIKGLKGRDAELQAMAVRNQAFAFRYLSQLSRALYAKVSFERYKALFSDMDEFIREIPEDFSMPWFLQHRARLLAASNGLGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0Y5</accession>
    <name>A0ABQ5S0Y5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006185</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="526" mass="58031" checksum="E04AE4ADC81E40C3" modified="2025-10-08" version="1" fragment="single">MNFYTSSRHWNLTYRSPGRPMIEDLEQEGAGSSNRHRGKFREQTLNGYDFTMKMKTPTPKKAVSTWTSVATRLWSLAWPLSGMEVMTFAKELIITAFVGHLGPVELSSLVLSQTLYNVSGNAPMLGVVTAMETFCGQAYGAKKYATVGVVTQRALVITTVFNLLCIAMWGKAESLMLAMGQDPHIAKAAGRFTMLLSPCLVLDGFEQCLRRYLASQSVVQPLMYVTFIATLLTPLYLWYFIFRCAWGFDGAAVAWACVQASSCLGLLLFTLYHTYGQEPSKRTWTGWSRECLSEWPLYIRVAIPSAVMICLDWWTFEIIVMLSGLLPRPEMTMSMMGITFNIHALCFFAAHGLSGGVSTRVGNELGASRPRQAWLNTQVSVLMGTVIMIICAGMLLLFRDQLGALFSGDREVVLLTSQAVPTLAISLIGEGANTVLAGVLRGCGRQKIGAQINLFMYWGLGLPFACLLAFRMGLGAMGLWTGLACTASLQSLILSWIVFKFDWNAEAQRAKALIASGELEIELEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S147</accession>
    <name>A0ABQ5S147_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WWE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WWE" evidence="1">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="157" mass="17357" checksum="D75B70424F28773E" modified="2025-10-08" version="1">MMEPVSVHTQYERTWYNNITAQSNHNEVGCEQVHVDIPPHSDAFLVEADMLANLNHPNIVRMLGVVTSQPAGDNTGDCNGGAATGVSDPVVGIVTEFVGNGTLGQVVRSPSGRLSTRLCARIALQVNEVVSSSTSPSRRWRWRWRWTIAHTGDWRMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2W1</accession>
    <name>A0ABQ5S2W1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Preprotein translocase subunit SecY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007444</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00303">
        <property type="entry name" value="SECYTRNLCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="478"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="623" mass="64980" checksum="026DFAFC621F488F" modified="2025-10-08" version="1">MSSMTSNHRMPVPGQSYSSLYTMCAFSSASAHRSRGQRLRGQILFLSDSLCQYHSKQRLLGQRHHLPQRSSPPVVGGACLKSRGFTYQAVGSASFGPQPSPLHGWRLGGGLLGSLARFYYYYREILTRAALTVGLLAVIRCGHFIPVPGIDLALVNTTAASTAGERLIKALYGQAQDIPASLFDLGISPFVNASILLAVALVLPQEVTTLPGLGWLSRLREARKEGRTGEALINSTTNLLALGFAVYLGLVRAFELEPCAVFGGGGLFLPQTVLALVAGSACIHMCASTMTAFGLGNGSSLVICSSIITEYASTLHTVLSSLDTCLLSPARLAAMLGGYLALVLASVWLSTAELRLPLVQYASSAPPPPEQRGGGSGLQDLISQARILAQKKSSERSQRGGMGSGLPLSLPGVGAKPAGGPGAGTPGAAGVSSKAGGARPSEYFPIQLNSNGMMSIVLGGAAYFGFLPRIVEWLGGGAGAAAALSAALVSPLGLLGYGLTVAALEFLPLGAINPKEMSEYFNMMNVGLKGVVPGEPTERELRTKLLQCKFWGGACLGALAVAAQLYDSACVQILGTNLGTTSLLIIVGAVLQTARQVEGLLEGPKLQRKLDQEREAIQSLSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S377</accession>
    <name>A0ABQ5S377_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chaperone DnaJ C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007584</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF553">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chaperone DnaJ C-terminal" evidence="2">
        <location>
            <begin position="17"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="188" mass="20656" checksum="B316A479DDCA2A86" modified="2025-10-08" version="1" fragment="single">PGMSARRPSGPHKAKAIEHKLNLSLEDLYTGVSKKMKINRKVRGGPAEEIVEIVVKPGWKKGTRITFQERGDEEAGVIPADIVFVVDEKPHPLFRREGSDLHCTVVLPLADALCGTTLRISHLDGTTIEVPVRDVIRPGDAKVLRGKGMPITKEPGSFGNMIVKFDVRFPRDLSEATKQQLKGLLPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3V5</accession>
    <name>A0ABQ5S3V5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_007117</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.380.10">
        <property type="entry name" value="Naphthalene 1,2-dioxygenase Alpha Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050584">
        <property type="entry name" value="Cholesterol_7-desaturase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013626">
        <property type="entry name" value="PaO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266:SF32">
        <property type="entry name" value="CHOLESTEROL 7-DESATURASE NVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266">
        <property type="entry name" value="IRON-SULFUR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08417">
        <property type="entry name" value="PaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="16">
        <location>
            <begin position="87"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI63951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="537" mass="60141" checksum="21CDF101BD927DDE" modified="2025-10-08" version="1" fragment="single">LYLTMRSLLSSPLQTNVLQSRRPLSRPLALWRTTSRTTTWSLDTIAATRTGGLTTEGGTAITLNRTPDISPETAGEPSSKFLWNAHWYPVAIVDDLDPRRPHATHLLGIPLALWRDKEGAWHAVEDKCPHRLAPLSEGRIETDGTLQCAYHGWQFNGSGSCTHIPQLRSDPKAMQTACASRRSSVRAFPIQVVHGLLWVLPDSTQGAHEQLAKDPALPKTAIKELLGGPEAEGYVQTTQWYARDVPMRFDTLCENILDPSHVPFAHHKVQGNRNNEKGTTTKLQGKINVEGFVFELDSQSPRFGSSLSFFAAPNYVRYNSPFRSLNVFAVPTRPGWSRIYVTFLKDTRRSRSFPWLVGALYSFFEKRTWLEHIFLRHPVLDGDNYILHLQERALHEEHGGNWKQSFYMPSAADDSVSAIRRWFDKYGDTVPTCESGTLMPPPLTKREALNRHSQHTVHCRHCKQALRNVDLATGVATVVGILAAMWLVTRVMLGLPLLSPAAGVGLFAVALAGAVVAGLRSLRQQFFFIDYVHAERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4H9</accession>
    <name>A0ABQ5S4H9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Polyprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">VaNZ11_007797</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The aspartyl protease (PR) mediates the proteolytic cleavages of the Gag and Gag-Pol polyproteins after assembly of the VLP.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0229">DNA integration</keyword>
    <keyword evidence="15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="13" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-1188">Viral release from host cell</keyword>
    <keyword evidence="14" id="KW-0917">Virion maturation</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="18">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="19">
        <location>
            <begin position="365"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="27"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="77"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="GLI64524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="57045" checksum="BB8B1D8A19B49727" modified="2025-10-08" version="1" fragment="single">LTLSSATAQLLAFEARVGSMHEASTSSSAFVSAGRGSSATGSAPRRGGSHVRGGSREPLQCWNCGKPGHLERGCTAPRMAQRSGSWSSAPRGPGYGSGGLERTRMGGHGEAAGGPSLGSDRRGYGRHDHVDGAGDRGNASPRNFAFVASGSDRLKGWYLDSGASHHLTYDADQIVDVRELAESEKFEILSVNGGIIRPTAVGTLRLIPGVNMHARVTVEEVYLVPDSFANVLSVAALDKQGGSVCFEDGQVTMRKGGEKILTGQRLGDLYALSCFSIPRSASRLRYTQPFGGVAHGKSASESMDLWHRRYGHLGLGNLERLVGEGMVSGVRFAKEQVKGAGDAGVCEPCVMSRQRRGPFPSTGHKVDQPLALIHLDVCGPMPERTPTGSLYLTVLLDDYTGFSAVAFTPTKEAVKDKVMTMVNQLETYSGFRVKEVRSDQGGEFINSPLQEFFRTKGIRHGPTVGYAPEQNGAAERLNRTLLEKMRALLAESQLPQCMWAEAVSTANYLRNISPVSGRPCTRMKLSQGNILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4R4</accession>
    <name>A0ABQ5S4R4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="308" mass="35297" checksum="6A4DF3C1C0856C47" modified="2025-10-08" version="1">MPQIEPVNPEDYNALRMRVHHLKQKHTEAVQQQHAQREVDLASIASRCAQLEALNESLLADNEKLQVQLRQSTERGAKLRRQQEREQQKLREQIEKLTSEAATVDENWAGKLAKAESEAALRVEKLQAEIADLKRQQEAEVERLKKEHEERLQDTDEWCRGEIEAVRHNARAQIAQAHGAVEQWQQKYDEESSTCRKLSDANKILQADVDELRAAVQAREGELKQANAVLLRMQKQHDAEQQGNEAEKQALKQRYTELLDSKRVEHAEQLAHIEGRLQAVVAKKDATISALRAELQNTYSTLLPLKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4X2</accession>
    <name>A0ABQ5S4X2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_008355</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF125">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI64950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="149" mass="15961" checksum="AF0620508DA59EA4" modified="2025-10-08" version="1" fragment="single">DVSGRVLPGQLLALMGPSGSGKTSLLSVLGGRAPSAVRVTGAVTVEGNPLSKAMRRKIGFVLQDDVLYETLTVYETLLYAGLLRLPRALSLDEKRQRVEGVMAGLGLSWNRDTIIGGFFRRGISGNCLCTGSTIVHTHSFYISPWAVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5S6</accession>
    <name>A0ABQ5S5S6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008734</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the jagunal family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009787">
        <property type="entry name" value="Jagunal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20955">
        <property type="entry name" value="PROTEIN JAGUNAL HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20955:SF1">
        <property type="entry name" value="PROTEIN JAGUNAL HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI65247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="204" mass="23604" checksum="85A5544AF09773D9" modified="2025-10-08" version="1">MSRRPEGTDGSDWTYREIIEDRYKRMAVNMSATFLLHQVQSAMMLLKIAWYMIPFFIEDRYPEPFMYAFLVFLVVGNICFYAGKPRGRCVLPLMKVAALLVMGTAALHFVALWRYHLLDPKTKHISRRVYKLLRDRDTISSPAYLNIFTVFEAILDAAVGLSAGLCFFTFNNWVKDAMDVVKEREERNKAVPVRPPPGAVKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S629</accession>
    <name>A0ABQ5S629_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YqgF/RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005227">
        <property type="entry name" value="YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317">
        <property type="entry name" value="POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317:SF4">
        <property type="entry name" value="POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03652">
        <property type="entry name" value="RuvX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="28217" checksum="31EEC1EFD408FB62" modified="2025-10-08" version="1">MSTFAVRAKNVFRYSECRKLHSRHCISGCTSVDRVNEGPSVHRLDATLSAGTSPMQISDPSSMNARPDPIPSAARSGPPLHVLGIDYGTSITGLALGRNGWCNPLKSIPSPRTCKLSDVARQVLDTALQHEVDGMVVGIPVQPGGSIVKPETDSRMGSRCRYLAHTLALMAKEYGISVYLYNEKDTTRATMKTLGYNWTDRLSGHEKQTRKLDSESAAMLLRLYFGNSRLAVRVNTQVNIKSTTDNPDPAGFIWHQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S664</accession>
    <name>A0ABQ5S664_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ferredoxin thioredoxin reductase alpha chain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_008960</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Variable subunit of the ferredoxin-thioredoxin reductase (FTR), which catalyzes the two-electron reduction of thioredoxins by the electrons provided by reduced ferredoxin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of subunit A (variable subunit) and subunit B (catalytic subunit). Heterodimeric FTR forms a complex with ferredoxin and thioredoxin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ferredoxin thioredoxin reductase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008990">
        <property type="entry name" value="Elect_transpt_acc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004207">
        <property type="entry name" value="Fd_thioredoxin_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044166">
        <property type="entry name" value="FTRV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46937:SF4">
        <property type="entry name" value="FERREDOXIN-THIOREDOXIN REDUCTASE SUBUNIT A1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46937">
        <property type="entry name" value="FERREDOXIN-THIOREDOXIN REDUCTASE, VARIABLE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02941">
        <property type="entry name" value="FeThRed_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50090">
        <property type="entry name" value="Electron transport accessory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferredoxin thioredoxin reductase alpha chain" evidence="5">
        <location>
            <begin position="37"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00026011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI65403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="112" mass="12086" checksum="F36DD975F56A1BF9" modified="2025-10-08" version="1">MIVHQATSFRSAAWAGRAKLVVRCSGNGASSGAFQAGQKVKVVSSIKIYHAPKHPEGIDLNGMEGTLIKDVTHYKGKILSANFPFEVEFHLPSEGKPAKFKAHLGEDEIQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S696</accession>
    <name>A0ABQ5S696_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyl-D-glucosamine kinase</fullName>
            <ecNumber evidence="2">2.7.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GlcNAc kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_008376</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type N-acetylglucosamine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.59" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052519">
        <property type="entry name" value="Euk-type_GlcNAc_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43190">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43190:SF3">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase BadF/BadG/BcrA/BcrD type" evidence="5">
        <location>
            <begin position="8"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="335" mass="34967" checksum="A22D888DD9FDA9EC" modified="2025-10-08" version="1">MVVVGVEAISGSIACVAVDADRNVLGRHTCQTQQQWDLMTAAQADAAAAELHSALNALIAVAASQPTANLWQSIEAICIGTTAADLGEESESNIRTAVRRLLPSSVQVLVYNSAAIALVSGTGGPLLGCVLVSGIDSSRAYGAVADRRTAAASGWGPVFSDGGCAYDIGLRVLSAISRAQDGRGADTLLVNAVYRQLGAQRAEDLIRWARSHQSINQRVSGVASLARLVLDCASRGDSVADALLRHAVGELLRAIKAVVAKLGLDRSRQPFNLVLAGPMLSDGTLFMQYLLEALKDGVPTADVIYPLGGAAEAAAWLALWLLNPRSPTPPLRRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6A7</accession>
    <name>A0ABQ5S6A7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="112" mass="12815" checksum="AC81C4C13BAED97D" modified="2025-10-08" version="1">MKMLTLGSLSETIPPGPYLHLKCARLKMYMSLSTDGDDNNDSYDHWRKKVKSASEDVRCTRARRQRGSSGSKKKQELLQEEVSKDEKSEDEQNDATSAREDDDNEDGEREDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6B0</accession>
    <name>A0ABQ5S6B0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_008513</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13681">
        <property type="entry name" value="SURVIVAL OF MOTOR NEURON-RELATED-SPLICING FACTOR 30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13681:SF24">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="3">
        <location>
            <begin position="604"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="428"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="499"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="748"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="924"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1003"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1371"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1651"/>
            <end position="1735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2063"/>
            <end position="2208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2338"/>
            <end position="2362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1155"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="439"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="531"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="748"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1003"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1051"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1069"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1081"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1103"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1371"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1445"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1675"/>
            <end position="1692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1722"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2063"/>
            <end position="2098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2107"/>
            <end position="2132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="2161"/>
            <end position="2171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2193"/>
            <end position="2208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="2338"/>
            <end position="2355"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="2362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI65105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2362" mass="238648" checksum="B773374D97BB60C0" modified="2025-10-08" version="1" fragment="single">MDLQAVAPGGGRRVRLKLFVKEEDEPTKICELNVRSGCEFKELQDLVTDNILGGEIERWKLAKETRTPHLGPNGAGHIEILQEWQSKAAELLTHPADDACPVTSVDDSDLRGGVDVYSIMLGHVARKPINSQKDLEAWWASHPRPGPPLLTAACARLLAKLRVAPSQHAPAATAAKLVSEGLAGLQELATDRWYGNRLPLDLLEKGLLTAMRWEQHAPSGQSAMLAGILEAAAAAAWALVSYGGPRSVLVGAGVVPELVAAMAKAQEWRKAALARGQAAAAAAHAELIRQTEAVAADLAAAAAAATATEAALEAKTAMGPELDQAIEAANAAADAAARMPLPNSRPIYLAGCYAAPAGEVSLETTNRIIYNCLCALAVLSVDARARSACLAVAPRLDFLLTAATTDFVALNDAQHELDAAAWQRRQDRRNAADAAGAERFQRRREEAKARKERAEREARERAEREAAEAAAAAALEAELAAEAAALAAEDSELAALFQSRAPSPSPMTVKGLATPTPAEDASAGGSGDGNEGPAESTEAIAEAAPEPEPEPEYQPPPAQPDPYPVLEFIAPEDDARVHQIVAAEALYAMLGRERGVRRSFAAAGGIPALESLLRSSNDSVVCAAVSALASYGRDVGADHTTGLDMLATAMPLTAAAAAEQAAVTTTKAPSPGPAARLRSVLPVVAAPPQPKKDPVVDNAGKVAASLIRVIPPVLEALMARYADGNREVGGMEPQGVYIDPATRAALATTEADTTSAEASTDGGDGDASAVKAPPLLAPLNARVSGVTTGGAAVLVTPPPATVTTAAAPVQTTVSLQPGATTQISAALSLPTQPTATTGVSGATTEGSKPPQIVRFAPHTLSYTIELASTALWAAIAAEQRATEIRAAAAAAANAEATAAAAEAAEAAAAAAAAAAAVPLTASSSVSSMDDSSTLTGQKNPTAAREAETAQAAGVAAATAAALTAMVEVDAYTTPITPVILQHIAHLAVAAAKLVPAEVEDLKNDTASETDAQTKQRPSDLSGGGGTRPDARGSSTRNDARASGARTGIWRVSARASGSRASGASGGDDVGPAASTAAADGGGDDLLDGASVESEDGGGDGDGQRSSGSSGSSVPMPHSHLMGSLHALLGGLCVIQGNTRLVRSEFKALLAKHAQLRRLHVQKELAEDEYQALLEEMEAARKQPGQFFFEHEEEAKREKKRAGIRNVEAEMRRELRAIADMEDGLQASADAAVRAVSATLTLLPYGGAAGAPILLEAPLQTSLENQLRHCHVLAAGVIQVMAATLQPGAVDAATAAAAQLQGNWETGTSVSAAAAAADAVTPAEELAAATAAVALYQGPYRDIILASGVLEALLAAATARFTAATTAAAAANAAPPTPMSSTAASTVAGLSNPSASPSRHGAVSRSSSVGGLTPRGSVGPRTSVATNASANAAAASGGGAAPSRVSISRSSMAAAGSRRQSSTSGEPGGGGGGAAATASRVAIAGSTGGGGSGTTPAVASPSRIRVASAAGQQPVDTSTNVLMQVTSCALMHLASTENALQPTQLVAIMSYMRTACYHTASTPYLAAACYSLARHVANRRCMVEGVRPARKGPLGLSTPAAAAGTSAPVATAFTSAAAAARASAAGSSLDADVWVEVVGVLLRGVLTKLRKPGGGAPRVTFQPTDPGVATAGAGDATRSTSPPTASTTAVRPSVAANPSNGPRPTSPRPPSAGSTHVTHGYANGIGGGGNGPMGPSTAAGTASGILRSGRFLLMSLWLLLRQWVVDKVNPVNMMSSADGLYAAAGFTWWSVRFDADTPLTLTNEVLDGLALVVEAVHAAAAAAAAGGGGGSAVGDTNTRAAAAEPALMLPFAPGEGGVTALRRLASRCVWSLSATHPAVAGALIDRGSGVLALAAMRDQSNAADSELQSLCCGFMLLLASRCSSLLPPLGGLEALSSALVVLVERALASGGCWPSSPARADLDATGAGGGSGVDVPLLEAAVRGLAYLAGSGLEGRMAVTGSRAVRRLVAVVRADNAALETVRQMRREEGVPSWSRLEELICPLRAINGLPMIVPAGQAASLAALQQGAQSPPGHAQPGYGTPQTQTQTHQQQPRNRPQSGLVPGGPQPHAQQHQNQQHQQSGAAGAGTTAASRPESSRRISAVVAAGGGGGVNHRVRSSRRGGGGGGGGGSSRAVGFADDGEAGGRRSSPTFQHQQGQQQGQQRQGQQQLLDEDLLVASRQSGEADVGLMALWALLNLSGYEPAQTGICRHGLYTLMAAVHGSADPARSAAARAILTNIHYHPGNATVLYKAELKLKYAALARMLEQERRGKEAREAALRRLAATALVGMNTGGSSSGVGGAAGNGGSGGAGAGGAAASVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6H6</accession>
    <name>A0ABQ5S6H6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyltransferase family 92 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044224">
        <property type="entry name" value="KOBITO1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46701">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE KOBITO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46701:SF7">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE KOBITO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="555" mass="61970" checksum="BC7F6936AD6C4D3A" modified="2025-10-08" version="1">MADGTCACPGRVSCAWGQAIGHHLDQVRCGPQEGRCRKHSQAQEPRGLHYCGRSYCLAARVRRLYPAAVYGGTWSHAARAQQLCSSAQEKQPNVDCNKALRNQSLPTSADLGLAPWDSMRDLETIPARIAFLNELTKEVCSNASGRVGAGIHPRVAAFTVWNGNMWGLRRSLLPWMQWSTQLGVCRFYVAYQGRDKQTLRSLEQIRSVSLILISPPFAPTEEVRSWRVHASQNEWAQKPGNWRLMAKQGYCGDIALARARAEGIQWMMHIDADELLLPAVSLAADLASVPAWAAAVKMHNHEALIESFEVNNKYEEVTLFKVHDATLPAKAGDLQWRFRLGTYSAYYQVYHNGKSIVRTDRGYVHTWGPHEFLGPRNTTWADPKHNPAGDFKSVTSQTTLLHVSYASWRDLMSKARTSCPSKFHDYAREGNRTKVKDCFCLDFDLDAYMAAVAGEGAARDYWTITSLFSEGAVRKDDEMCKVYRDVPRLKELLIRDRLLIRVTGPQQALRAHEVAIRALARSMGIRVGAADTAVGSGAGIQKSQPQGVTETVRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6L5</accession>
    <name>A0ABQ5S6L5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">18S rRNA aminocarboxypropyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_008252</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002621">
        <property type="entry name" value="PRK02287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase L inhibitor RLI-like possible metal-binding" evidence="8">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="16S/18S rRNA aminocarboxypropyltransferase Tsr3 C-terminal" evidence="7">
        <location>
            <begin position="174"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="36103" checksum="13E0108B92621DF1" modified="2025-10-08" version="1" fragment="single">MPRPKGGGHHRPRGPGGGRGGGRRGLSRGEQAAEAWEADDGAFAGGESTSAAAADAYTQARARRDRFPPAQIAQQRYKSDLAVADIDADSNGDLEKDDDDGTDGAAGSELTTSVYAINGSGSGAASTAAIPAKFPVHLAMWDLGQCDRKRCTGTRLARQGLVRELRLGQTFPGIILSPAGQRSVSVEDAALIRCRGLAVVDCSWNRLDDVPFGRIKGAAPRLLPFLVAANPVNYGRPCKLSCAEAFAAALFICGLREEAVAVLSRFKWGHSFFSTNAELLARYATCVTAAEIISAQNDYLTGRPAAGLLSGKSDAEASAALAQAVLDVAAAGRWGPAASVSDDDDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6U5</accession>
    <name>A0ABQ5S6U5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TATA element modulatory factor 1 TATA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_008610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23160:SF19">
        <property type="entry name" value="MYOSIN HEAVY CHAIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23160">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="114"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="230"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="282"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="410"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="460"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="731"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="780"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="953"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1564"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="532"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="862"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="991"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1352"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1651"/>
            <end position="1719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="744"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="781"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="953"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="969"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1755" mass="186565" checksum="2595117B6D483052" modified="2025-10-08" version="1">MADISAWAVHAARLSDAMKKEQADQFLRGAMAGSYGHQQRAVSSGRHPRLSGGSPLHGQLQAASEMLYGVSEGSHQATNQSPRPRSVSVGRRHQDMYELVNVMNEISSTGQEIRNMTRRSQEQQSPLVPGRRASGAPGGSSNDGRIALGPGATGSSTAAAMAAAAAAAATNGRSYTSAGSVVAGGGSISSGLRGATGGGGSSVSGKGFARSILGGVESLTTKLMILDNKLTPRKVSPRSEHQRDTSVGRRNPSSSGEGPNYRDMLAEVNAGLLQGHGLLDTVQGSRDQRPSASSLGQGGRMAFDPPSMRPSVTGGSSVAGGGATGSNYGSTVAPDWAASLIMANTQQRAEELQATDRMLESIHAQIRGGVSAGAAGGPTTGAAAAAADAPGQTAVTSATLSSALLEAGAGAAAGGGSGADGANRNGSAPRRRAAAGSIAGGGMLQPFLATLSEAMPLPQTPVIRGPLAGAETQQGGPAQPVQSRHLGPHVPAPPPPIPRGLPSGSATSSPVSGIAGPRSARNQQRYDMEYRQQQQQQQQKQIQQQMQQQQQQKQVQQMQQQRLQQQMQLLQQRHERQPQSGFVAPADAAAPLQLAPPPAERSTTTGEGSMSTADIDATFNQRLMDLQTIADNLARSGIVTPEVESSAGSAGAASGEAASLLTAGRRRDGTMVAAAAAGSGPLRREGAASGAAAFLLGPRGAWLEDAAAATLPATTAGAGAWALPGSGVGGLGASRDGDGKSGRRSGASHLGGPSASAGVGLSGASGGAAAISSGYTLAAPHQTTGSSQGSQWPEDGGSLDSLHMNLLMQQRKKERARREELCMAESDAATSRARKEAADLQAQHDKLVTQYHAERQRTEVLKAELERVTKDCARRKEQLQQLSDLTANSVAQTRELQKQLQDARAREEEMRRQVEHANTAMFDQAARQKATVERALQEFLSLLESVASSNSSISISNSTTTTCSGRSTGQILGKSSGSHNGDMESMLESLRARLREQCDVARRQKEMLDAAEAKLAEERSSSASARETASAERAGEVALVLQQANFQRARAEELQKELGHQRELATKLDLRVRQLEDERERAVRQRDAAEMSLQAQQSQLEAAQQQQDVLVGRLYDGQRAKEDAEVLQTQIRSLGRQLEEARAAAARSEANERKATSECEAREELDSKLAVLQSQLGEREAALEASRRRIRELETAANSAVASRQVATEEVTKRSEELRKLTAENGRMIQHLDRLQQERRGLEAKVQKLEAQVQTLQQDMITAQGQLSAAKDQLAAAKESSVVLDATLAQHQLSADLAAARSEAAALRAAHEAASEYAQTMARTLRQHGLSPPPPPSSTDVVSQIGDLQAKLSAESSALAAAKTEVSELRQQLADARSEIERLKRESQYMADRYSQLESAHANSQQSWQHAASVDAEELQKLTKERDALQRQVHSLQLAGNHEADELRRKLARVEVQVESLSGSKREAEERVRVLTLELGALRKTLGDKEAQMEAMRLALEDAKAAASQAAEMQLHAETQVAQVERARLAAVAEADKARTAAEAERARAAAAAEVEAMRAKEEAERAKRSAAKAATAERAREEAEQGRVAAAAELEKQEEEIGRLQSEVERLEKELEDRNAEYAGLYDYCGQVIGERDAMMEEREAFSASAAAAAAEVAALRQEIEELREDLADKEQRLAVKPKMAELQARINELSVELADLKGERDAAVAENHQLSSKLQEETEAHFQTDGRRVRLGEEVERLRGILKMYSIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6W1</accession>
    <name>A0ABQ5S6W1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="279" mass="29485" checksum="75B03F02985394E2" modified="2025-10-08" version="1">MDITRAAQLMSAMWPDVECAVCKVEDGILLDAAVMAAIMCTLKEELAPLEDSLAAAIIDVMETFVLRMPRPKGFVFIPCAVNEVKCLKDFLLDDAVHLLPTGEEDVSPRDAEDVTPCEDDACKPPEKTAHNITSARSSHTSEGALPGEQYRAGVLGGSAPIACVQVLTWRDQLCAPDTIGMALAAVFSGDTLRSAGPLGVVYADLQEALSRQDQSTLDRAQAAAEHLPLEPLPGHVLGKHMCVADIWEAMASVQGSRLGLGGMAVGRCGSTQKPSGSMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S745</accession>
    <name>A0ABQ5S745_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_009139</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="4">
        <location>
            <begin position="83"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="404"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="900" mass="97432" checksum="6AA64AED2A9C9923" modified="2025-10-08" version="1" fragment="single">MSSASQVAADFASLAKFPDAPTFLASEAKSASLWHRRLGHLGLTNMKRLVQEGMVKGLEVTDKELREFEGRRCEPCILGKHARDPFPASEGKTQKALQLLHLDVCGPLPLSSCGYKYFVTMLDDYTGASLVQPIKEKSDVANFMERAITMLQVQSNGTLQCIRTDGGGEFVNRQVQSYCASNGVRHQLSAPYTPQQNGKAERLNRTLLDKVRAMLAESAVPKEYWHEALMVANFVRNRSPVAGKPKTPYELLTGVVPDVSLLRVFGCVAYTHVPKGKCDKLDSRAQKGTFLGYEANSKAYRILLGDGRIQITRDVVFLEDQFAFSNHVRPELEVFSIRDGPSVPVAGAAAGNAGQAGGAGVVDAGVPAATHAAGAPAGNAGVAGVGATGGAHVIPAAGAHAGDAGVAGMDDGEVPPLMEDDDEGEEEDYGMSETEGGGDYGDDAGGDVASSSGSETGQGSSGHTGESGNLDPTPPRPIRQRKPPGWQKDYAMLASELEMKEPKSLEEAQSRVDWPQWKAAMDEEMASLQENRTWELEEIPMGARKIGLKWVYKLKRDADGNVERYKARLVAKGFTQKEGVDFGEVFAPVGKYASFRALLAVAADRDLELHQLDIKTAFLHGELKEEVYTEQPPGYNLGGPNVVCKLRRALYGLRQAPRAWYIRLRKELESMGFKPSLADPGLFVKIENGEPVYVLVYVDDLLLACKSMAVIGQVKAQLLRAFAMRDLGETRFYLGFEIERDRTARTISVTQRRYISNILEKFGMKGANSHSTPMDANLHLTSEGDPLDTSVHPYSEVVGSLLYLAVCSRPDISFAVGSLARFMAKPTINHWNAAKGVLRYLKGTQALGIKFGGGAHVQGYCDSDLAGAEGRRSTTGFVYLLNGGAIVWSSKLQPTVATST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7J4</accession>
    <name>A0ABQ5S7J4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="164" mass="18474" checksum="77FCD5D6883DA31C" modified="2025-10-08" version="1">MMVLATNAARIHQKEEIQAARRRQAKSPQSSKARNSSRNATNSSKSAAKKVTNDGDVRPSTKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRRKKVKSASEDVQCTRARRQRGSSGSKKKQELFEEEVSKEERSEDEQDDATSVREDDDNDKIIFVCTETILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S831</accession>
    <name>A0ABQ5S831_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M43 pregnancy-associated plasma-A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_009458</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M43B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47466">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47466:SF1">
        <property type="entry name" value="METALLOPROTEASE MEP1 (AFU_ORTHOLOGUE AFUA_1G07730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046224291" description="Peptidase M43 pregnancy-associated plasma-A domain-containing protein" evidence="4">
        <location>
            <begin position="28"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1077"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="276"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="741"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="845"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1141"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1250"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="848"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1176"/>
            <end position="1189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1311" mass="137101" checksum="E5AFCED2876A06E5" modified="2025-10-08" version="1">MFLEVGLTTGRLIILCQLVWLLRSACSQQYRSQLSQVASAGQGSPPEDFIKIIPEGAARPFLALKDPDNAAGQDVQRLYEQVANISSIGYLFFAMAEPLSKPHGSTAALLPYLSPTVLRLYASHPERCSSALINQMYDLSNALYGSNSMKACHYDLYIALREVFSWVRDNIPLQLQEVPGQPLASAMWPDMAKYPAQRLTAAVANALSNPAKLGDTALLLNASCGLSPRDLAALVPDISSVLKQLHADLPLLDQLGKKLFDNLWTDVVPNVIGGGAAAPAEPGSGRRSQQRRLAATPSATSASSSTSSVNSDSAGSSFFYSMPPVGFVPAPAAALPPVLIPLIFHIMLYVDRGPSIAPTQYAQAPVYVERLVRVANTMSRPANIQFFVKEVRNDPTTYLYLMLPSRVAWLTCPSGGSFSDQCLRNQTFIASVVADFPRSINVFVASDTTVMTTYPLGYSWTPGSDVIPGRGFVFMTWDGLSTSGSNSLALYNDGPNTLLHEIFHHLGIKHTFTAAAAAGSSSGASQGGGCVDGDYVIDTPTTLGTIGSSNFAATATAYCMELFWGQYGGDWNATYIRWSSTLGIPDTDMNAWADSCPGNPGYDELGNYMTYNTPVCFAALGHFTEGQAQRAHYVTSELNPVLYAWGQYYAAAAAAQVPMGSGSPPDVYDNSCTTTRRGCPCKSSWSFNGTAYSFCDRMFKNDTLFCEVLNATTCASCTAVAARGTCRASCAGTPLMCKKPPAPGTQNPPPPPPRPPSPPPMPPPPPPLNVPPECKVSESGCRCRSLWQIDGSFYSYCAIPSGAVQLQCQVEASCPSFNPLSPLQLCATNLSNLGCSAGRIRIATSVQPAPPSPPSPPLKPPTPPNPPLLPPAPPPPSAPPPSSAPPPPSAPPPPSAPPPPSALLLLTPIRLTGNANCYLSQILPVHIKYPFPSGNQLYDSQYTHALSLSPSLSSSLPISLLPFLSPLLFPTSLSSPPSLSVPKHTCTYLVSHSLPFLMRKLRYPTSDPYNAHPSSSLAACTNGGVGAAPPGACRDAPIQPPGITSAMPASAPAAGRPAAAATAAVESQQQELSANQVIGIAAGSGVAVLLLLALMIAALVWHRRRSRVQTRISPSAANVAWEGQANNQASSLTAAARNVVGGSGSSGNGSRDGSDVTAEAASTVHGRASATADVTPSPPPPPPPPPPGSQPVRIPAALLAAAPGTAAETPSDLPSPLSFPVLPPAAVAVAPGIMSHWLLSPAPGRRLAPLRAGRAPASPPPDVIATSAPYQPLPAARGSLGQSDEVLDEVSEQQPPSVLPGGVSGSAEGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S840</accession>
    <name>A0ABQ5S840_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BLUF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009796</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055308">
        <property type="entry name" value="TEX47-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34035">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34035:SF1">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24787">
        <property type="entry name" value="TEX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="641" mass="67818" checksum="5BAF8F82FF56403D" modified="2025-10-08" version="1">MLVQSGHATKHKLLLSPESLSWDPGRVHSVPSTHRRKHVEAKRRGGRPQCSSSPQQWRSVSTHHPGPSRKPPGSRLGDGPPTPPLLSAAVWALPALVAFNLLRSLVLMWVNGQRRSSRLRQASGSAALAAEPTAVRTSRSGQQPPAESPSSPVATVSAGAIAPPAVAELGNGAGSSRSAAVGEVATTQPIAIREAAVAAASESAWRSLASEAEEGLSGPQPPTVAAAERSAVAAAAGLAGPPIPPGESEGSARQGNDSAQIAGYQMRPRGSKSNSNLGRVVICATLELDSEGHATGFGSGSGFGSVMADLAPWGSVEWHRRFWEPLLNKYADSPADRVTGLLVLYGRSVLHILEGDNNQLFALLRELRQDGVGVHRLQEIRVPCYILDLRERLFDSWQCATAVQRHMGRHRLTRAAFKAPVAAAIAGAMTGGTGIHAAATKASGPGGPLPPLAGPVGEGSSETAAESGVAASPEHQLVTRIHQLELFIKFVGPKLSALAGPEARSQALQNLYSYETTTPPEDVVGALATDPLAPELHEFVTTFDADYHRYVSLMALVAQLEEEMEDEAAVTAAGGRARLAARASSLIGEVSHLARFYEEHVRRDVREALAQREEERRSRGLDEVTRRLSQGMRLRVLEGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9B8</accession>
    <name>A0ABQ5S9B8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009397</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="667" mass="71594" checksum="6F7879274BBF1C55" modified="2025-10-08" version="1" fragment="single">NDHFEWLLYGTHKFKPSNEPSRKQIIGNAKLEFKGKPFHYNAKLRPLVLRAAKLLDLDEIQAILLLRRWVKNHDINEDTIQENDPAAMNTMVLQVLKYYNLERHLLLKSIQVVLINEAEGGTLADLRSRLLNGNMEEQLHARLRENVESGAVAISRRLRAGDAAAFAGAAAGPDAAAAGTSTAVAAASTSFITTAATLDWLKAAKDEMQTGLIRERIELLNCMLLLYERCRVACKPERALALARLLLDRVFASVQPADGNSGGSGSTISFTTVGYGVRASAGNAAAEADVQHSRMLAALLLMSCLDLPGHILLTAGPAVVSDGAAAATPGLDPPLGGKTMDLHNQISQYAAVPETALLLLTWVGCLRLLDLSGSGNSIRDLDASTRDLEGRVAAIGGLAPAAAALKTACDGAFGIVLLQYRGVARKALCVLCTAFDLGADSLNPAMYATVEELLTLCLQDDVQACTDFWDESVATMAPLRSLRTSAVRLFPMLLHPLLVLLRLTAVSAATASRAYAFLQEKVSLMVVHTADEPALKHLEDAPNEVLVDVPLPWNLAPTVEGMVLPEEMPGTVICMPPVLSALQGRFIIVEYAADVNGNCPQSRGQVLLLSRAVYCVAALEHALRYNLPLERSLFRDLCDTFSLLGSLCALHPDNLSDIMAQSVTSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA09</accession>
    <name>A0ABQ5SA09_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="54265" checksum="7E0EB6F301207B60" modified="2025-10-08" version="1" fragment="single">PAAAAAVAAAAEVSAAGAALFTEAAEAIRVDPGWIYLMDCREALWALWALKVAAAIYSPTEGIASEAATPPILRPLATKVSYTPQPLVELQLLERAASQLDQLDPGQLAEAFAVAAEAGFGADRDMLSALRRCTLARIAAVRPQPLAVMVASLAVLQIQDVTWLTALAAACRNQMINMKAEQIVVVLHSFAVALRFYYLPLFHAAAVMCTLPGFARLEGMSAGEVVRLSAAYAAVGHYEAILMRRAAERILALGSDATCWQRASVVHSCARLSYRNNPLLRAVAMEAPGLPAGPLALVAAAYGHLQCRPRGLLPALHSARGTEWKKMGLTQRAALCWSLLVLTGGLAGRTSDVNGGGGGSISRAAGADFNADKDGGADDGVDVEIEWEDEHKATATATASGMPIGPRGSDLMWRRRRQHRQLAEALSGYLRALGATDWRAWPVSDHHHFQLLMACSVLAWRGGGQQQPQGLPKKEEKEEKEKARVGDAGGTAASEDRGGASGGNDSDGVNGGNGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SA25</accession>
    <name>A0ABQ5SA25_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021047">
        <property type="entry name" value="Mannosyltransferase_CMT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34144">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34144:SF7">
        <property type="entry name" value="EXPORT PROTEIN (CAP59), PUTATIVE (AFU_ORTHOLOGUE AFUA_7G05020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11735">
        <property type="entry name" value="CAP59_mtransfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="520" mass="58209" checksum="F860114E94B64531" modified="2025-10-08" version="1">MLLRFRVRRSRLQRLGAAGSRIGVVLLFLVAIRHLYQGGKLSSADANFRAVKASAQSLSTIQDSAGLRECFALLADRKDAGSDLCIRANNFLSRLHAAGLNIPLPPGGNVFERHGICAGACFIATNLRDAEQVMPNYIYHLLLTVGLLTHYGNPAFVSVYESGSKDRTADWLQVLGDVLDALSVPHRLELGGSIQREKGEDRIHFLARVRSAALVPLFEQCNATGQFCAPSNDKSNSSGRIIFLNDVLFSAADIMRLLQYPADLVCGLDLGMAAEPDMTEQEHHATVAAYLHAKWLMPQHLAIQFTRLSLLSKWWRKYHHKRKPLLREFGPLFFYDKWVARDISGRMFTNRSPYVVYQPSVDRVTAGRPVRVHCCWNGLAIINAEPFVRKVLGFREHREGECAASECSLLCDDMWRLGYDNMVMDPNVRVTYDLAGVNKLFHAFSSELPLGAPGLVYIPFRQEEVRDVMSLPKHDDISRESSNPDGHRTREVDCCDLKKGANMVEFRDPGVCHTSVVTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAC1</accession>
    <name>A0ABQ5SAC1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000042">
        <property type="protein sequence ID" value="GLI66820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF17">
        <property type="entry name" value="SELENOPROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="498" mass="54029" checksum="E57F3322EF7A8FAE" modified="2025-10-08" version="1">MRFLWSRGPARDYHRLIELKEHRQDLMVNPDVRKCNSSTAWKNYLIPCLVTSAVILLLFLWASSEHSIVARLRLDMIGTNGSGQGVVTGGGGLGRTAQAGGLDEERVRRRAVGAVLGALVADAATMGLHWVYDLEKIRELISEQGRAEEPAFFQPPSCPYYEYESGSLSPYGDELLPVLQYMTVGPGSHREGTMDGPGFAKFLAEYYFNYTGRLNKSSRSMMEALLQGGHGWPECGDPRDTQANNFVKVIPLVARYAGQPGLVKAVDAAVRAQQNNDEAVMYGLVAAMILERVVLGFSITDAVQWAADGAAGSGVSLSRTALEALQTVIRHRNSGTPLRDMLYAPGSSSKTGSWGPSCANPGALQAALLGALQEWQHLSIHLASSLGSRDLNAAGSSSNNGEGGDDTVYARGVRANILLGGDNCSRGILLGALLAAEAGPNAIPFSWRAQTRTYHSSEVLVEHLMEFRSSGVRRRNQLLHYRHHRLDHQHHSRAAAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAQ3</accession>
    <name>A0ABQ5SAQ3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010471</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000037">
        <property type="protein sequence ID" value="GLI66635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27348" checksum="C6302B34253A01F0" modified="2025-10-08" version="1" fragment="single">QEIGLRLLPSDEDRVTACQFADDAEVALEGEESVPAFLEAMDTFARASGQRLNLDKMEILPIGAVDDHHQGSTINGLKVVTTATALNLPMDNQLQPPQPDWPEMLALAYTRMQRLAHYPLSAFGRAAAVSNYCLQTVTWHMEHGGPPPDTTLTYIERLAARLVDRKQGPEDQGQRFTGVPSRLLVGHPSTGGFGLLPLRQHIRARQAMWAVRFIRGALMARRERERVMLLDTAMNTVAEEEEEPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBQ6</accession>
    <name>A0ABQ5SBQ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemerythrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35585">
        <property type="entry name" value="HHE DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_4G00730)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35585:SF1">
        <property type="entry name" value="HHE DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_4G00730)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="1">
        <location>
            <begin position="92"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="279" mass="31599" checksum="0931BFB61B3561B0" modified="2025-10-08" version="1">MHECRMHIYPRLLASSDWTGIWNFGAWCIFVVEAAHRDRFHAAVLGHQTFQPPRTSGLLKQGYPVATQVLVASSPRIRGMAASDVQTGPSVISMINEDHRLFKQLWDEYNGPNMNGEMKQKLGWSLIRAIAMHSAAEEEIMYPEIRKRLGPEAADHLLGDDGHQAIKKLLFEANYMSVEKNGEAAYTAKLKEAMKVLFHHIEDEETRTWPRFESLPGVDADLLSHLGRKFDAAKHHAVTRPHPWAPNKPPLNLLANTMTSPLDGALDMWRFGGNPPRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCH0</accession>
    <name>A0ABQ5SCH0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_011852</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI67607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="253" mass="27726" checksum="1741FA29F2931CF4" modified="2025-10-08" version="1" fragment="single">IIPLNWTAKVEFIVTSFSSFHGVDENPTEQLAKWLRTSLEEGRLAQLGLAVRDIKILRVAAKDVDAFFAQLAHQLEQAAAPGVMGTTCTGATGNVAGKDPITIRTSDEDVGTVMKRPRTVLLHLGVASGATEYRLESRAYNCANFRVADEGGWCPVMEEIEPGRGSSLWVGSRLPLQTVCKHLVAEGHNVVVSEDAGRFVCNWTYYRACSLAERYGTEAVFVHVPPFEVFGEEEQRAFLIDVMQVIAGCLNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCX6</accession>
    <name>A0ABQ5SCX6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6817 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011586</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049202">
        <property type="entry name" value="DUF6817"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37391">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37391:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20680">
        <property type="entry name" value="DUF6817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6817" evidence="2">
        <location>
            <begin position="59"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="439" mass="48380" checksum="5844101EE79DCB1B" modified="2025-10-08" version="1" fragment="single">FTTDSMSNTERPDYEVGGGNGSASEPCNTKPILPGIVRAFLRGDLSRVDSRLPKWLDILSGVGAGECWHKKSSFKSHLFEVYKICKIWGQDDDVCKCALFHSAYSNSYVNLAIFKESTDRSRLREEVGTSAEELIHTFCVVPRHNLVYEDLLARAPSAEELAVLEGAGSGTTLAQSSLRRLIPPEGITVRHIKTGEPVTVDRLTVARFLVMTMADFCEQLFSWQDAMFENHDGRLLYAGANHGSLWPGACKPGLWHSALSRMAALLRFACTGPDGRPLVPLPPVFEGCTQVVTETDQLAARDAYWSAVSELTEPAQHTDALRLLRTACWHNPHIAEPHVLLAQIYCQRGQWADAASHADRAVWLLGCWGTSWDKRMPWDAWMAMARVLAHGAAQQVWPTTSFGMLNLGLVPDLDEPYEVDGSTETKGQADANGAEELAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD21</accession>
    <name>A0ABQ5SD21_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="5">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011266</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="5"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="8">
        <location>
            <begin position="30"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="95"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="552"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="668" mass="73171" checksum="DDA588077B95DAF0" modified="2025-10-08" version="1">MQRLEILARQLTAGSATNDRTPLLASRDVYDSMHRKSVEDPEGFWGELALGYHWQNKFPARGHMSYNTDVGRGKIDVQFFCGGTTNIAYNCLDRWVAAGRGDDPCVLWEGNDENERRTVTYRQMLEDVCRLSNWLTSRGVKKGDSVTIYLPMVAELPAAMLACARIGAVHSVVFAGFSAEALAQRIVDCQGKVVLTASGVMRGAKAVPLKAIVDDAAKLAAKGGQPVTSVLCLEVPSALRAANTPWDASRDVWWGDAIPIQPPTCPPVWLEAEDRSFLLYTSGSTGKPKGVVHSVGGYMVGVGESCRYLYDMRPGDVVFTTADCGWITGHSYLAYGPLLCGCTVVILGGIPTHPDGGRCWRMVERYKVRQFYTAPTLLRSLLQLGDAWPRQSDLSSLRILASAGEPMNEHAWHWFHEMIGGSRCPVVDTWWQTETGNGMITPLPATWFQAKAGAAGVPFFGVLPVILDPATGKELPGNAPAEGVLALRGVWPAMARTLYGDHERWEQVYFAPYKGYYFTGDGARRDEDGHYWITGRVDDVINVSGHRIGTAEVESSLTEHADCAEAAVIGVDHALKGQAIYAFVTLRGVDHQNDAMRKTLMDHVRKSIGPFAVPDTIHWAPALPKTRSGKIMRRVLRKIASGQEKELGDVSTLAEPGVVEMLIKLRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEC3</accession>
    <name>A0ABQ5SEC3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09271">
        <property type="entry name" value="RNase_H2-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013924">
        <property type="entry name" value="RNase_H2_suC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47204">
        <property type="entry name" value="OS02G0168900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47204:SF1">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08615">
        <property type="entry name" value="RNase_H2_suC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="16673" checksum="20A3E7424A42E25E" modified="2025-10-08" version="1">MASSSVITIQTGLSNGYMHNLPIKLANNTTSAKLEETWTQGQGRETDQAFLRGRLLRGARVSLPEGYRGFVIRSNNSHISSDECQRQWTASSEFSSFTLWAHDVAPGPSDPFRRALEWCALSNLVHTVVSLEETEQELRSIMSTTASIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEN9</accession>
    <name>A0ABQ5SEN9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cysteine synthase</fullName>
            <ecNumber evidence="7">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_012279</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="8">
        <location>
            <begin position="47"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="359" mass="37813" checksum="9284BD1356D6224E" modified="2025-10-08" version="1">MLLQKSQGVARLRSGRSGFVRAPRAAVVPKAVATFEKAVDMNIASDVTQLIGKTPMVYLTKVGDGCVARIAAKLEIMEPCCSVKDRIGYSMIGAAEQDGLITPGKTTLVEPTSGNTGIGLAFIAAAKGYKLILTMPASMSLERRILLRAFGAELVLTDPAKGMKGAVSKAEEILASTPDAFMLQQFQNPNNPKVHYETTGPEIWEATNGEVDILVSGVGTGGTITGTGRFLREKKSEVELIAVEPAESPVLSGGKPGPHKIQGIGAGFIPAVLDTSLISEVVQVSSDDAIEMARRLALEEGLMVGISSGAAVQAAIKVAKRPENAGKLVVVVLPSFGERYLSSVLFQSLRDDAAKMTFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SER5</accession>
    <name>A0ABQ5SER5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_012274</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF35">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="1414"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2145"/>
            <end position="2292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="72"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1942"/>
            <end position="2008"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1112"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3006"/>
            <end position="3068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1942"/>
            <end position="1961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI67943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="4223" mass="472994" checksum="D7847BB40C9420DC" modified="2025-10-08" version="1">[encoded data, 4223 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFD6</accession>
    <name>A0ABQ5SFD6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15032" checksum="EA31E9F95BAA549E" modified="2025-10-08" version="1" fragment="single">VSEHWTVEAHRPPIETPVAEHSAAPPATSALAAACYVTLERDAAAAAGGPHGDCPRSSGGGGIFRNCRFVFWARHQARDVLARVKQAGGQEQYSVGPSTTHVVARYDTSAEEASQFLRTSDLEYDTDAAASLLLPPGVLFVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFX2</accession>
    <name>A0ABQ5SFX2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="140" mass="15530" checksum="E4D0516E79B86346" modified="2025-10-08" version="1">MSSQRTLAPSSFSFASSWNGIEPSRKRQHVGRRGTAQHTLDARLIYMHLAALVYLSHGSSAQAAALAHKGNYAFPAPAATVSSDPWLHKPPACERLSRDQRLAIGVFNPEETRRAMAHRRHQVLCDRAWESENEQVSSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGD5</accession>
    <name>A0ABQ5SGD5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="114" mass="12611" checksum="E79D35339E4CB4E4" modified="2025-10-08" version="1" fragment="single">FLTGPAASAGFRGLKSVVTAKSKMGIETGFSTGGAKGRRTASSSRLSQISDGGRHGHRDLTVAQLTILRDTTDLVRTFWLEMEQKRWCSLDLMLVYELYTDPRAMKTLYPVNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGM7</accession>
    <name>A0ABQ5SGM7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_013316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI68814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="182" mass="19880" checksum="2BA5D7DC754E8C2C" modified="2025-10-08" version="1" fragment="single">MDDQAAFQLEGRDVGLLQALLHGALLRTSYESVHVQRAVEAIVTMLSGGIQVEFQSNALRQAEQDDDHMMRESICGKPSSLMELGAQVSKAAERVSALRRRGAQIVMPALSADLEALRPVQQSGEVTFLTPGRSGRSPNMVSDAVRCDNTSNDKLHKALDKLPALRARLDEVTMRLANVMRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGQ2</accession>
    <name>A0ABQ5SGQ2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF10">
        <property type="entry name" value="OS12G0136300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="638"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1030"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="638"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1140" mass="118311" checksum="DCAC92BB72254466" modified="2025-10-08" version="1">MPCAVADSETSLEPAAAVNADAGASALELLQPSHSGSSAGVYGIVTEFPYSYEQASTAAAAAPMSVGLDAPPANGFTTAADNGLDRAGVWERGKKARPGSIQAYGDVGGGAVPRTDVVQLGNVTSADEGGVAWGADEAASGSGTAQGPQQSLTTEVTVMRDHASNANDSHKSVTGHPTPLAAPRLRGFAAPPSSLTSELSAAVLAVAFPGDSVTARLRRGLVEMLVGRLRPVPLAELGTLLARHPDLGKLWNLPAYRLPKLRDFIESTAALAPRALILDRGDSSVWVVCDPEALRLAALRRAVAAAYPGDDDVSLVRRAAAAALVDTESPVMGLRHSLHMPSLGVCVVQMACEPRQRLAEARGTAFSLASVLQGPRSADPSQATAATAGNGVPRGEENGAEEGYIFLSSVGPCRVARLDADRLLLDAAAGRLGDLTAVQPVKPKASVAGSSFRAPSPPADGTAPATLIVPRVKEVLRAAIPEGRPEMHAKRCIALLAAASLGGTRGPSGPSDPPHTLRSSPCGIVLGDLGLKPPKGQMRMLFDDEPHVFRAGKPSPSYLIELVYEPLLELSISHPEDLAASVAALLQGRTYLGLRFRAPGRHGLYSFETATAAAASAAICSTTAPAALAPSAPSSRFGAAASASRPAAPRPSPPPGQPPDYYQRLEKAMLERLEGSSASEFLTRARHILAMALLRRTGSHGGPSGASDGIPRHAMSVKEAATVLKTHMPEAYQDQRPPGVFPVKVQLLAEQMPLLFTMMIPSGNPKAWYIRLNVDSLELSAGLNPKLVTQLEDAAESTEGDEGEYLEEPHGGDANDLHAMPAIAAARAVELEPLPPLAAAVAEVPAMQPARALAAALAPMAPPEFLPDPCVQIITMPYSHEHVTALSHCQSCSQIGLAAKRVGGAPSIVLLYAPAVDTLKPAAASAVNGGASASPVPTGILPATVYVLDASGAAAAAAAATGSGSSGGGSDAGVALLVSLRVLLEDSGVAKVVHGCEQLIPMLEHAVGCVISPMLDTRLVSEMLKPLDLHPRLPMAQDGPPQPDDGRAPELWPQSSHWQTALSLKYQDYHMAVLHHASGADSMWLTRPFNEGQVGLLVRAVQHLPELWSALTWLLPRLAQFCSIARAQQCRAILGSAASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHE9</accession>
    <name>A0ABQ5SHE9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M11 gametolysin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008752">
        <property type="entry name" value="Peptidase_M11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05548">
        <property type="entry name" value="Peptidase_M11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045672570" description="Peptidase M11 gametolysin domain-containing protein" evidence="1">
        <location>
            <begin position="36"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M11 gametolysin" evidence="2">
        <location>
            <begin position="129"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="462" mass="50922" checksum="6FB39EADE6012675" modified="2025-10-08" version="1">MAPVPLQRPFAGSFETYFVAAVLFLLIGLMVPCSSEQVAKRVSLQGKLNYKDAHVRDIWTLVQSGTGVVYKLPEQPLDSLGYPIPATSIVMLDCTPDPKMPTNCTKISRAEVSRAAASLARVGTQTTLRLLVMVVSYMASPSCGARSGASVANLRYAYTRDLGYMNFLRNCSYGQVTYSNVTVISTRVLCTAFLDQCDEESIAFTAKTSAANQYGPAFVSSYSRFAYVLPYGMLSACGWVGLAELSGTQTWYTPDGDGVFNKGTVLQESLHNFGIYHGWRNGSEYQDNSTAMGLGNSCPSAPELRRLGWASPLAQLDNSTLPIRTFKAFTLPATYVTSEGNMVRIQPDWLLPKAYKKNLYLALRMQGGGDRDLLDEFDGKVSVHEVNKSIDNFMAVGDPRVSLLRTIDPSTVLDMPTYKLRVISGALIGSTITVQICRYDKLPKECMEANLRRERKHRRVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHK0</accession>
    <name>A0ABQ5SHK0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="103" mass="11379" checksum="F3B3724FF3029CEE" modified="2025-10-08" version="1">MPTLEEVQSLWVEATAHLAGGSDSQTTAPKSSRPSEGWQSVSKKPRTDHTSDKPPSTRCRYCHGFKKPGQSWPDHVVHCSKAIKAEAEKKLALAARLESKMHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHK2</accession>
    <name>A0ABQ5SHK2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014032</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019458">
        <property type="entry name" value="Est1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045153">
        <property type="entry name" value="Est1/Ebs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696">
        <property type="entry name" value="SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15696:SF0">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN EST1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10374">
        <property type="entry name" value="EST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomerase activating protein Est1-like N-terminal" evidence="3">
        <location>
            <begin position="145"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA-binding" evidence="2">
        <location>
            <begin position="308"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="696"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="771"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="878"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1073"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1127"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1204"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1666"/>
            <end position="1705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="776"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="803"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="813"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1011"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1073"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1679"/>
            <end position="1692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1758" mass="178954" checksum="5C9BA2C5A103C2D0" modified="2025-10-08" version="1">MRPSSSGTMLYEPSSPKAGRHNGFPFNPPPLTPYRGLPAPHTGGSAGTLAGTAAIGAISPQAGAAMAMSAAAVGGSAVISGVGRQHHPPAPPLWDQVLELEKRLRAEMRKKSFWDPDVRRLRATLQGTYEALMFGHYDFAYANEAEPNLWKAVFYKPIEEFRSRIRALETLVRGTAGSATSGVSMSSGAGGGTSTPGAAMLCSPEEARAQLGRTTAAYMRFLDEALAFYRKMVWKLQWVHGNVGAVVDLDAALQMEIQDCVPRATCPRTPEARQSVDRCLIYLGDLCRYQSMALKDRAASARPLWERARSYYRQAARVLPASGNPYNQMAVMSYYTNDELRAVYYYFRSLAVSMPFATARENLLMLFEKNRNRYDNIASSHATAALQGGHEADVRTAAGHVADVAVRFVRLHGMLFDKINLHQFPEVLAAAMRDLETMLDNPGCRAILQKAPDADQLLFHLAIMNIFSVHSVSAGGGAAGGEGVAGTSYAAAAKRAELMCNSLTVAMRFAAIVTSVAASMAGIAAAGGGVGDVETGFGAASAACHVMLAWIVSNPEVLSPKPAGTITGSGSGSGVGSTEVPPEVAAEVTARCAFLTAAARLAMHLSDLTRRRAHDQDWQDPAVRHRSLPEDSELAGFGPLAPVVSVAAAWPATSESPGGVAVFAVRVARMLQALRHLDDALGPGLLLAATAAGPSASISPRVPHSSTTSPWHAPAASVAVPPPGSALAQELTRAVRGFRNAVVRATVIHAGQQASLLGGGSGNAAAAAGGNLVNPPASTEGQMNVHNGLAPGNSSAAATASGLKGGASGNGGQQQQQQQRLLNGGIEQEKQQQQSKRISHSGMDVADAMLDNIRGSEIEDRIMTDAVFPTALLVPAQQQHPSGLAQPLPPSPQMLNGGDASDTVPPHQVNVGGSGPTCANYPQRTDHGELLPRANATVGDSSACSVGGASGIGRTGTDDDGVEDEIVYQPPRLPRPTSTGNGLQYLHQQSASGGGGMGQLQDGKSALPKQPSGSSAAPISQLVSREASRDGLTMQPRSPSILELNMSPSAVAAGAPLAMPSSHQGIALNIGNTGGMEDAGGSGRTCPGSAHGSTLPLSPKHQFVHQQQSQLQPSQALPFLKLQANQLQQQNPGRGHISPSIGNGAGGSATGMAPAAKPSAPMGPNEKLAQPQVPFADELLVAMQQTLLRDGDVDVDGSLGATRLPSAPFTASGGQTVGTPAPADGTGERPGSDANMSVRRPPAAGTTSAPSGANALPASTPAVTTAALALHPHSPQVLQLDKLPQTQQLLQGPDASVPITGVATVSAAFAGLPASLQQRAQKNQHEEQPQLDNQTGVSGLLLLTGGSQVQRPSAAGAAASLGLGQGPGPGQGHTAPLVLPLGTFQQAQGTVLYNGVTSSCTAAAFPGLPVPLTVPRLTGGTTVSSGATCPTTCSQLGWPTSSVVAANGIGNGLDMSSYFTVAAGNYQAAAIAEATAEATAVAMADELLQAMDEDDNGANGRSAGGTTGFGMFGGGGSSRLYGGEEFLDIDRFSPAMAAAAAMGASGGAAPVTREHSTNSDAIFITAASGGSASAGAGTATGMFACHSSGSGSGGGFLAGVLASTVSAPIGGYRASADGAGTAFLDLGLGLGPGNTDAFGARGLVPTVVSERAGNYYQVLRRSGGGQNVSAGGAAPTPQPGSQAQGPQRQVQETGPAAASAGHGGVGNGGVFGEDLSCLSYLAAQLQRPHVQMQGQGKGLTWQGQAQGCHLPGPAGAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHR4</accession>
    <name>A0ABQ5SHR4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014129</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0072">Autophagy</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="4">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="229"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="681"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="916"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1041"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1144"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1260"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1388"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="772"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="689"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="922"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="967"/>
            <end position="976"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1431" mass="149089" checksum="9A91326E069508A3" modified="2025-10-08" version="1">MASLLVLVAHSGRTLQLDVQASTRVDAVQHALVSFTGIPVADQIAMYQGARLDPAKLLGAYRLPVDPAVVALEDHPVFLYCRSYLKSGAALPPPEALPQLHVQVPPLAEVNLAHPLHSAPSPLVRALPDYERHFQHHLATTRAYWDVAQTRLQRCRQLLSEQEVQARAADAARANVEAHYNYILNSYQMFLDKYNGQHTAHAALLATFPADLAALEAIELHPSLHQQRGLNEESCPSHPGQPNITTSSGGCSFPAPAPLSTPGPQRLADLYPVQRLREWAETCSRSHASFGAKVSELEALFQLLRKDVEGLFMQAPSVDLDALGRQMADHEALLDEHSSMVQVLSKDLRTVRTLVEDVVRQLGSAAGGPGGGSLRQGGLHDAAAAMEAIHETHRNRVLPRLAQLDTQLACFAARVEASKVAMSRDVLSQLQRISGQQSRIRDMKNKLAAFYEVLSRQDAAFAELRVVHRLPSAYRALLAEVVRRSAWHQLYCVQAGKLAEHMARFRDKEAGRRAAFVSRVARYIPNDLLARAGLASEPPHCQVTVPPPEVPLISVAMADLQRLSYLDERMLAASLRMSAVPPLHSDASSVHHQHQHHLHRGNVVDPIAYGSHHQHQHQSQASFRLGGSSTIPYPGTVAVLDADPPPPTDSVNALEMENALLRAELADYIADSCIRGLNAATPSNSGKEQQPAAAADSSSAQQAGSAPPTSTAGSASLTPRNDRTSGGGAGGESSQSVAMPAVAGSPSAPQRRDPAEVLAAMRRALGMKDDFIRHQQRERAALASRAAAYEERIAALELLLMQRARGSDGDEVECEQGMATATRHMESSSGASAMTKGMSQAARAQMEFNGCCGQGEAGGHQPGLSEAVVNAAKEGFEGGDMAASGRPGGHGAEGSAEVVCGERDNELRALPSLAARVDTVGNPESANHSSFSMADETSDRPKGDIGLEGLAVSHDDSRDAPLPALGMDDHHTDSERPSTSPLSPFSVEAAQQQLPFPRSQAVDTVSGALPCGAEGLLSTAADLEACQPACVDDAETTSADAAAPMHGSCSGSGIQARRKSSGPQSIPQVSGGLRRGNAAPGEEPSLQYEPHVPMSIGSLPFATSLPVGSTLANRLASSPSGGIGIGIGLALGFGLASLAEAPGRMPREVDGNRSRNNSGSSRRTGDGGGGALARTASIDVEDGNPEADNVTTPDSPGVLQGSSGGASSGNLQFQPSWSAQVSGDWAIGAAGSGNSAVTTGTVDAGRDDALNVTIAAEREGGVSGSDDEIAEGGGSVQEEPDEHAACDIGVNNGATTHVGQSELGASGAALECTVTDVGGPPDHGIGNVCDDGPLAEDVPDIDEIFDSDGAWEVASEAAAASPAQAATAATEAVVVVEDSDIGVGLVAEPSSELGEGSKEGSRGVEVGSGGVQVAQGMGTSSGYESVGSDVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHR8</accession>
    <name>A0ABQ5SHR8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013877</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05467">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15048:SF0">
        <property type="entry name" value="STARCH-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="3">
        <location>
            <begin position="134"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="71"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="539"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="403"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="234"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="588" mass="62258" checksum="AB0BFB59E8EC232A" modified="2025-10-08" version="1">MLKASHCSCQRPQVAGRGSSKTSAAGSPSPQPSFPPSSSHLKWGLLVPTQPAPLTSVFGRVLVSPVAAAVSTTTTTSAASPKSTSVNGTAKHHHHHANGNGHANGNGTGHSNGHGNGGGGDDASGADLPDAVLLRDAATVRVHLVVPRCPTVPGEHLVLVGNCEALGAWDVHKGVRLHWCSGHMHTATVVVPADVHSMKAKLVLVQGQGVQANVAWEPGQDRSIVLQPPATAAGSSSSSSSSNGNGRSSKDGKAAAIRGTAAHDYVVICHWSHTEATSVLEREVPGEVLMLEQQLSRAISELRTARSAASTSQEKLQRMAQDLATSERAARQSAEQLSELQMKHSAMASELTQVKHSLTDAKRELTEVKRQAKAAATEAANAAAAAAAKASSVNVSEVEARVSQRYEGRMAELKQQLDGVKSEYSSEVAALQKKLEASLSKEVEAQEMVKTLREIVQKDVARLRTELAQLVNEYVAARQQVQEQAQQIEASEKLRMQQIDGLSSEIKNLITKYNNAQHTIAEQQRLLEELQASRGVRLPSPRAADPVQHMTVPPAPDSQAGRGWRADWARLSKLTVNGAPATAAGTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ27</accession>
    <name>A0ABQ5SJ27_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_014630</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03755">
        <property type="entry name" value="proteasome_alpha_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1 2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="4">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI69978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="254" mass="27392" checksum="7BECE241003E3C5E" modified="2025-10-08" version="1">MSARYDRAITVFSPDGHLFQVEYALEAVRKGALAVGVKGQDTVVLGVEKKSTAKLQDARTVRKIVKVDDHICLAFAGLTADARVLINRARVEAQSYRLTLDEAVTVDYITKYIAGVQQRYTQSGGVRPFGISTLIVGFDPLGTPQLFQTDPSGTWSAWKANAIGRNSKTIREFLEKHYVDTSGKDTVKLAIRALMETVEAGSKSIEVAVMERGTGLKLLTDEEVDALVKEIEEEKAAADAAKRGGAGQAAGSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJA9</accession>
    <name>A0ABQ5SJA9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cation efflux protein cytoplasmic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003511</name>
        <name evidence="11" type="ORF">VaNZ11_014520</name>
        <name evidence="12" type="ORF">VaNZ11_014543</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000088">
        <property type="protein sequence ID" value="GLI69816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF52">
        <property type="entry name" value="CATION EFFLUX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="8">
        <location>
            <begin position="78"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="9">
        <location>
            <begin position="278"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="388"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="393"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="406"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI69816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="50684" checksum="ED694632FD722A3B" modified="2025-10-08" version="1">MAQLCNSVASGQTISIVNPQAFLRNERSLHRNSRSLSLNGADVEAAPHLQEDNYVQSLPSATEDAEKFVRQIRIGINASWVVNIALLVVKIVAFVLSGSYAVLASAMDSLVDTLSQAVLALAEHQATRSNQRFPIGRTRISELAVLACAAIMFVSTAIVIREAIGCLVQGFHGHPSQVKAEAILFIVLGAATAFKLALWLYCRILMKNPIMLALAQDHLNDVMSNVAAIVGAAVSGFRPKYWYVDPLVALGMSLVIIWNWLVICWDQGQKIIGVEAPVEVVQQVKEVACSHHRDMALDRITAYHHGSNVVVEVDVVMPSDMSVRDSHDIALALQHKIEAIDTVERAFVHVDYEQRDLEEHKVERNLKLGEKDIMKPLPGYDLLPLLQHQQMKPPSPPPPPPPQPPRSLRLQQQSVLAVSSSSRLPSTSVVPIPAAVSSGSRGSSAGSPTVFVRPSLLPGIADGTPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKH2</accession>
    <name>A0ABQ5SKH2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle 14 kDa protein</fullName>
            <shortName evidence="7">SRP14</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_014728</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer with SRP9; binds RNA as heterodimer. Component of a signal recognition particle (SRP) complex that consists of a 7SL RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SRP14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.10">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, srp9/1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003210">
        <property type="entry name" value="Signal_recog_particle_SRP14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009018">
        <property type="entry name" value="Signal_recog_particle_SRP9/14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12013">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02290">
        <property type="entry name" value="SRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54762">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, SRP9/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="99" mass="11419" checksum="A54D4BB35F0DC235" modified="2025-10-08" version="1">MVLLEPDPFLSELHKMYERNKSGTVWITVKRTNMKPRKSKKPDPKAEYVCLIRANDGKRHVSTTVGTTHYAKFSQSLVIIMKGAMADTLKRKDKKKENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKJ5</accession>
    <name>A0ABQ5SKJ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000091">
        <property type="protein sequence ID" value="GLI70179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12145" checksum="075BAECDFCB793B6" modified="2025-10-08" version="1" fragment="single">PDLGFALSMLSRFMAKPTKPAMGMAKGVLRYVAGTRNLGLQFRREGKEIIDGYCDSDWAGDTNSRRSTTGYVFRVNGTAISWSSQLQCTVAASSVEAEWARPFPAPSGKSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKR1</accession>
    <name>A0ABQ5SKR1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">adenylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_014713</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="149" mass="15283" checksum="84CBC9132FB6BFE3" modified="2025-10-08" version="1" fragment="single">KRAESFTDKRNKAAVPAILGVASKATSSSVPSAAPDPHKPSGSVLTMGCGASKADAPTKPLENKETDMGNKDSKPAAAVAEEGFALPPVEGELLDGTQIFFVLGGPGSGKGTQCDKIKQDYECVHLSAGDLLRAEVKSGSVVGKKCEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL01</accession>
    <name>A0ABQ5SL01_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015379</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="434"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="483" mass="50065" checksum="3AA849D864213C18" modified="2025-10-08" version="1">MPKPQKAKTSLAEMLAKHREPPRNDWSNGRRGADANRRYDDDGFPKNDPEGFKSDLEEEDAGGPGAGGAFAILASLSGGGAEDDDWIAVAAKQKKKVAAPAAGPAPGAPRRPTAPSPPAVRPVGPIPAVNPPRTLGVEGGVLGSAAGPSRAPYSLAQGTTAVLRPARSQSHDAEGRPVSDTYYQMFQKVHRDTSGNVYMRFHKTDVVMVRPSGDVVLTSGGYRTRTTFLSVQQALQPLGLTVHSSLGGDGRGEWSVGGPDGSRTPWEDGMVVPASDEADRGRGQRLLAAYNVGTPSVSADIAARAPAPAPALMTAVPSPSAARAPVVVPAQAPISTSTWSYRSAAGISKAPAYASHIRPPTAAAGPLAVAPSPIQVPVPVPTARTAESISELQQVLNAALAIKDETSGRTSGDSIPYILRANDNGAELDDEHACIACMAALKTTLLIPCGHMVMCGPCAAQVLERSGVCPMCREQVISQVTVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SM79</accession>
    <name>A0ABQ5SM79_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000102">
        <property type="protein sequence ID" value="GLI71103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="12728" checksum="0FBAE18B80D98493" modified="2025-10-08" version="1">MWSCLPPPLPPALARMSETEGAATATQRFDAVHLRFVAKRTDGRTKLHGRCRAAAGGGGGGTGTGVAATAVARGMWPWTWSARGGGRGTRTRAYGSEGGDTASPPATLSGSGGDLFPTSCGAGAPIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMI6</accession>
    <name>A0ABQ5SMI6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592:SF15">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="2">
        <location>
            <begin position="61"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="161" mass="16008" checksum="A38807534FF2E96F" modified="2025-10-08" version="1">MLWSATAAAVSLRTGVDLLHLSGAGVVPSPSASPAPSGADLTAGLIFGGLLQNPGDLGQWLRAMAISAISPAAAEELLYRGFLLTAMQQRFGAINATAIAATHLSLSQFFPFILLGACCGGLALASGSVLPAVVAHASYNAAGIVVGLVVALGVRVQTGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNF8</accession>
    <name>A0ABQ5SNF8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="263" mass="28339" checksum="4E2592D6A50D0E02" modified="2025-10-08" version="1">MTRYVNCGSAVVLPTEPAWPFGTRHREPELPAGPGTGKYPGAMDSWNAGTALYGRLSGQAGGPERPWQYSSLPYSGGRYLKPDYLSQNPKAPAYGFGQRTGKGDAPTPGVGSYSPSTNTFNFPKMAHSIWGPPPRGRPPSAPIRPRPEPEAEDHRKPKPNSAMPGATFKGKHFPGWPTDGPGPAYKPCCGGHCCDMCEKYKGVSFGIRHHIHQEPVTPAPGYYRVAESSLGAAAAGRTAEYDGTTYYHKPGRKDHSYANGLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNK6</accession>
    <name>A0ABQ5SNK6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="245"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="528" mass="53661" checksum="CB9E627DA811CA6F" modified="2025-10-08" version="1">MSRQNQRYPSPFGGGRKQPDGLNSQSYLSGTGVQAALGGLGYGVNGRPLPNGANGIPYLQPSIFANTKLGSGRMNNDPAAPTNNPNGAAAVPAAPPGGRPTAQPPGRNAPGTISPKISGTFTVTNANTIARKNSGHANGGTMLGGNNTAPAGTLAGLARMVASAVGGVASVGGAGASGPAGLSVIGAATRGGGGPGMSATLPAGRGTNGALNGGVAGNGATVSRPPTGNGMTAAIRTAAAVPGLAFSVGSNQSMGSRGYQEDYRVICDTTAAPGLTNLPNMLLGAAVYDGHGGKQVSHWLSSSYCLLNRAMESVQQLISNGGSPNDVAAVLEDVFIACGNAAIAARMTAGSCVAMALLIQQAGVPWVLSSNVGDSRTIMVSWDEHEAGGRPEAAQLSVDHKLGPAKLSEETRRVQASGGTVIQLFGTWRIDGSLAVARAIGDCEFSRYVSCRPTVNCRQLMRRDRWLIIASDGLWDVMTNEEVAMFCKAQQQLTAAKLSEALVREAVRNRGSGDNTTVVAVRLQYNPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNR9</accession>
    <name>A0ABQ5SNR9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016386</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="34235" checksum="8E11372EF96E2B7E" modified="2025-10-08" version="1" fragment="single">MEAATENNGPAAKLSPFQQHQGEYGILQGGTGAAPLPSGAISKNCCGWQLPPELWSRIFVHLAASLEPPTGLRGPGVVAADILRAGLTCRALLQASIAGIKALATAVPIYLHTLPLLLLPSKPAHRHHDHHYHHYPATAPPAVSDTLDTKAALEGSPETEPERQPDWAVIDEVLTRPISCTKAQLCNALRQLRQPNEGTRAEMMSRLRSLLGLAPAAAAATEPPLRLPPPPARLCVALQQEREERCGSAAAMPLSVRLVAALRDMVEAGNGLVGAALAEPSLAAMRRELCAFYGSTEGLMAAHQTCLQQLPELRWRREVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP57</accession>
    <name>A0ABQ5SP57_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotide-diphospho-sugar transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053250">
        <property type="entry name" value="Glycosyltransferase_77"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46936">
        <property type="entry name" value="ARABINOSYLTRANSFERASE XEG113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46936:SF1">
        <property type="entry name" value="ARABINOSYLTRANSFERASE XEG113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="3">
        <location>
            <begin position="153"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="731"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="636"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="744"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="772" mass="84233" checksum="80F4DDE7FB10BBE2" modified="2025-10-08" version="1">MTPVRVGRLSTRFQVRAILLVIFSCGILISGLTFHHTEEKYSYHFSTERIQSVALNNTLLVTAVDLPLWEFFGPSWVLSVQSASISYWLIAAMDNDTAAAVKAAGYGHRCVLVPKPEMAPSKATARVKRKRRAAGGGGRDGGGGGGSLPRHAGYSWSSITWRGATWGKVAAVAKLVDLGYNVVHSDIDVTWFRDPLQLFLNLAERAVIQVRTRKSAATREGWLAAAAAAGGPSSSSSSSPSALFSTDLLTTANPPTAGQDSGLERSCDPSYSLNTGVYWVSGAEPGARLMRGWMALREASDLNDDQQALNVLVRGTAMELRAVSLPAVYDLRAALAAHEQQQAEGGLRGGRNAPKRLGVPRAQGGELRHTYLAAGAPGVAGAEVDGVVVSCLPVSSFSHTYAFATSRLHEARQHPLYEVHWVWGGKSLESKRECMRDAMVFADPPDYHHPHRVMTFELEHQEMPHGYNSWPSSRTSEMVDFALPALSAQLQQTYWALALAMAINRTLVLPQFRCYCAKNWFATSACRINSERDTVFPFPCPLSHVFRSKLLLSGELRVADSQPHPHLRPSSSVQGAAWRVADVRESAFLNNPRTDERLRIATPYVLRVRGSLGDANNARNGSSSSSSYGLSHNDNNDNNDNNDNNDNGSSDSDSGTGIREIVLDRPRTDRDLQDLLLTPEYDAVRVLHVPRPATVMSGFADALMWQQYDTAVQRVTATWCCHSIRETALHGGSRRERLQPLPPSRQQQRGFDSTSSSSNSSNSSAAVAAEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SP73</accession>
    <name>A0ABQ5SP73_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Hypoxanthine phosphoribosyltransferase</fullName>
            <ecNumber evidence="5 13">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_016309</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="5 13"/>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050408">
        <property type="entry name" value="HGPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005904">
        <property type="entry name" value="Hxn_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01203">
        <property type="entry name" value="HGPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340:SF1">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 13" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="14">
        <location>
            <begin position="115"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI71194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="282" mass="31285" checksum="B74D363078CAFF33" modified="2025-10-08" version="1">MTDNMMQSSAKSCRRVLSRLSSISTARNKSYISTTANNSGGCFHYPHHVPHASTSIIARHPAQRCLPILPPLRSLSSAETPLETPPHSQPPLHPRRLPIHPEIDSVIFSEELIQARVAELGRLIGQDYELSDLLVLGVLKGAFMFTTDLVRYIYPYPASLEVDFLKASSYGSGTTSSGVVQLDSSFKKEAVRDKHVLLVEDIIDTGHTLSHLVTLMRDAGASSVRVAVLLDKKARRSTPFQADFTGFECPDEFIVGYGIDYAEKYRNLPYIGSVKKEFIKHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPH8</accession>
    <name>A0ABQ5SPH8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="9">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_017208</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="9"/>
    <dbReference type="EMBL" id="BSDZ01000125">
        <property type="protein sequence ID" value="GLI71815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020552">
        <property type="entry name" value="Inositol_monoPase_Li-sen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00378">
        <property type="entry name" value="LIIMPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0452">Lithium</keyword>
    <keyword evidence="8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="267" mass="28390" checksum="F65748BC8980DF6E" modified="2025-10-08" version="1" fragment="single">MIKSTPTPLLSHKYADYVNVAVDAALAAGEVIKHAFKQPKKVEEKLNHADLVTATDKAAEDLIFSRIRRSFPDHRFIGEESSAATFAAGTSELQLTDAPTWMVDPLDGTTNFVHGFPFVCTSIGLTLGGFVVVGVVYNPVLEELFVAAEGGGAYLNGERLMVSGRTQLTGALLGTEVGTARDDETLDAMFGRVRQLVGRMRSVRCTGSCALGLCNVALGRCDAFFEINFGGCWDVAAGALMVWEAGGAVLDPAGGTWNVMARRVLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPJ4</accession>
    <name>A0ABQ5SPJ4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="130" mass="14568" checksum="00755ED80F3A8C0C" modified="2025-10-08" version="1">MKKCNGKRDKRRDDKPPTFSRWEKTELDGLTKRLQNTSIRPQPKQDQHASAKAGGDTAKTNGPLLDKNHKQEDGRAGSSNEAGLSRSGPEQNWMLMLLKTMMMAVVMADTTRTRTAKRGKEEHDEDTDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQG2</accession>
    <name>A0ABQ5SQG2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="17808" checksum="A2AD6E98B3369A17" modified="2025-10-08" version="1" fragment="single">TKCNSLQERFANVQKSMKLYRFQSRRLKRLLKEAHGQALDAADDYIFLNDEDDVHMAERMIRFEEPVQAESVSAALEPPAIAPAAIQPTTPTKLEERLHYSYKSRGSKSTAISAPCSWGIRDTFVEVFGSRICGQASIAAPSKSVFVKLLEAAAGQPSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQM2</accession>
    <name>A0ABQ5SQM2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_017191</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000125">
        <property type="protein sequence ID" value="GLI71825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="418"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="172"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="344"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI71825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="719" mass="77032" checksum="4CAA399351AE2E33" modified="2025-10-08" version="1">MEETESPTLTPSIGGATSYRGSSVTLPHNALASGLNGRNSSVCDILSTTIAHTGARSGAGPSPSLFAWDVDGTHTHALVSPGMLSGKKKGLFHGLKKMFNHYGKTDGRAATGQGQRGTNASVTSSDGTGGTLDPSISRKIDRSLKSPSSHLHLQANSSRDLSLAALLTSPVRGGSAAAPSSPAVMNSPMPSMRFDHTSSNINFTLSLADGDASSPTAPGEQQQRLFTNFKNGRHTAGQVLYTQSATSLSVPTSKPSWGANPLQPGILQQAMHQQQLPPGIHQHPGTLHKSYSTALPTATSATSSSNLSILLARGDSVDDYRSSGSGTSGRVALPYIASPTVTRQDGSYHGGAAAPLGSGRANAAPPPPALRDVDSTAPCEEDYEACEEGVDQADNLLLGMNPAVPPAMRRRIWTLDDYQVAKRMYKGSTSAVYRATCMRSGITVALKVYFLNKVPSNVVHMIRREIELHVPLVHRNIVMLYAAFQDEKHIVLVEEFAARGDLFGIHRSMNCRLTETQTSELILAPFLDALSYLHARGIMHRDIKPENILFTNEWTLKIADFGVSINMHDERAVTRTGTADYMAPEVERCPLKHAPEDNKNNPNLAYTPAIDIWSVGVLAYEMLVGFPPFVSNGPGEDSSSRQGVAAFLAEQATRKTLRFPASISAAAKDFIMATLNENPGDRPTAQQLLKHPWLFKAARRLSTSSTSRLSTSNLVAVQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SR50</accession>
    <name>A0ABQ5SR50_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="200" mass="21471" checksum="3AC962FC6450168B" modified="2025-10-08" version="1" fragment="single">GEHVAAVRLVQLLLQGAERLGDANSVSVAQHAVRSAVHFRQRHSRTDPQTRAWVLARAHALLLQDSDQDPGRQEGRQEQEEEQDQQVPVSEHSKNKNNSIGADADASVKARENPWSIVAESTRELVGDMSAKSPATCESDNMMAIGGADGGCSSNSGCFHGYSGRLPKRGSITLGWRGIPEAQRQASVLPLSGLSVTDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLB5</accession>
    <name>A0ABQ5RLB5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036418">
        <property type="entry name" value="Cyt_c_oxidase_su6a_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81411">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="127" mass="14431" checksum="F551E5B52009221E" modified="2025-10-08" version="1">MQALRRSANAILPGLRRMGTATDASINADKYWAPFFPKPQQTAEQVKKNVSKEMVGFLLLGPVGAAFMFYDFFVGLEEEHHVTIPPYPWMRIRRAPGMPWGEDGLFENHPRVAKIWPPEEGSEKGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMW9</accession>
    <name>A0ABQ5RMW9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000653</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.200">
        <property type="entry name" value="Cullin, PONY binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042460">
        <property type="entry name" value="DCN1-like_PONY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF2">
        <property type="entry name" value="DEFECTIVE IN CULLIN NEDDYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DCUN1" evidence="3">
        <location>
            <begin position="56"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="254" mass="29319" checksum="AAD886A3511ADB15" modified="2025-10-08" version="1">MNRYTKQQKDKITQFRTITGSSEKIATDCLKNTGWAIDAAIDHFYTSGLATQASGLDLRAVEALYQRYKEPDDDAIAVDGITRFCEELEVDPTDIAVLVISYYMNAGVMCEYTKDEFTSGLIKLGVDSIDKLKRRLPDLRAEIRTDNKFKEVYAFAYNFSREKGQKCVMLDTAVAMWQLLFSVPEQRWPLIDDWCEFLTKHHNRAISKDTWLQLFDFIKSVKPDFSNFDENSAWPYLLDEFVEYMKNKRGGGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN77</accession>
    <name>A0ABQ5RN77_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47908">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47908:SF2">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="33451" checksum="1E3F53652962CC60" modified="2025-10-08" version="1">MDCAFSLYHQMYPRPPRLLRVRQTEHSHVHVPHRARPGIMSSTSLSLCPSAASSRTPDYAASPSGRSTPQLISRRSLVVGAVSTTAIVAGFMAAGGVALAASVINGGIGGGGDPRTLTRSGMDKFRRNQVEASVEDFDLVVQLAPEMRPYMWQRGLSLYYLGRYAEGAEQFRVDVAVNPNDTEESIWTFLCEAQMVGPEQARMQFLEVGRDPRPVMRAAYKCFRTGELPDKIMAQVSDNGGHDTFYGLLYVGLWHEAHGDTTEAKKAITAAVQTPYARLSGDYMASLARVHCQRRHWEASSPASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNM7</accession>
    <name>A0ABQ5RNM7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001000</name>
        <name evidence="3" type="ORF">VaNZ11_006955</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="2 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="170" mass="18339" checksum="3454C53E201C5A0C" modified="2025-10-08" version="1" fragment="single">VDISTQTFFTFEKDTQTPSRRRRDGQPILPIAEQSPSTNHSAAELPRDADGDDAMEASDGEPPAKRVRAHAPQAAVAAVGAGPSSASRPHKRGKRPSSGGRGNARGDRRGHQPGRGSGVKPQSLTADQERELTRYYNRYGRQLTRGDLRDLHKEAGGNVLYAGLITVCTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNV7</accession>
    <name>A0ABQ5RNV7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_001097</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01049">
        <property type="entry name" value="YJEF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="8">
        <location>
            <begin position="100"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="381"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="387"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="274"/>
            <end position="280"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="316"/>
            <end position="320"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="467" mass="48603" checksum="E1E23550E7ED4384" modified="2025-10-08" version="1">MKACCFQFNSVAKFAPASLSTVCNRAGTRAGIKVCHKVSKLLGFGMKHLPCFLSQRHVRLLNAHHQDARALKKDTLISPTHVMATMGSTFGGADTDTDQQAVALFRQLVPTLGGNYKGAHGKVAIVGGCLEFTGAPFFAAMSALRVGADMAYVICTPSAATAIKSYSPELMVLPYLPEQPLGSGVDPSMVSSADEATVSGAVARILSWTSRASAVVVGPGLGSNPGVVATVTVLMRETRKKMQPLVVDGSALTHIVAKDPETVQGYRLCILTPNVAELGRIGEAVGVQLPGKMSDAWLQFAPQVARAFHGPVVLAKGEVDLLCGPGGADEGGPGELQPLMRCTDPGSPRRCGGQGDVLAGTAATFLGWAFKHQQLHAQNLAAAEAAAPPPNASPAQPPQPTQSPEGITRAPGSNGLDEATVALCGFMACKVMRGAARQTFLIGERSMVAGDIIPLLYPTFKKVVGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP59</accession>
    <name>A0ABQ5RP59_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.310">
        <property type="entry name" value="membrane protein fhac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PHEROMONE SHUTDOWN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530:SF7">
        <property type="entry name" value="TRAB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="510"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="539"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="712" mass="74251" checksum="1F3A1B41B529CA1A" modified="2025-10-08" version="1">MQGGSLSSAPKAGRGCRQAFRSGAASWPRRGILTCRASAVLEKSAKTAGPTSIALPPFLRNNVVILEAPNPTAPGGTTRVYLLAMSHVSKRSVEQVSDLIRLVKPDVVAVELCKERAELLVDPQDAAKPPKVWHSRKVQIEGLPADDSDIWPSQEVLLSLLVCKPGRPVTQQDIEGDVLRLLSTGLFSSVKPGASNAGRDEAPGFLVRPGSEGAAGADERCLALVPPLGCIRFKAQARGLPAVTAMSVRVDSSLKAVDVDQTALSAICLQASDEGKKGADGMTTLLRTRQRVMELLSGHKVAVSFSGVETGRVEMLVKAVKATDPPFVSGLEGTAVNGEGFGIEPFRPQRGLVKVSKKIFIPQETIDAMRAERMGAAAAAEAGGKGEGEEGKGDPHRSTVSPHTRGVGGSSAHPRVRASLRPWSPEELAAAKQDEPPRRVVQDTLAQLMSTVYARIQSKAGRTVGLEPGAAWRAALEAATSVGCGTVLLADRPANVTQHRMGAGLAGDTGLRLIGAAALTLGSLVVTLGTSLLPDQADGGVLAAVLAAAVAVVLPIVAPFVEVSRFADMSEDQIEDAVAVKEPIDQGDLSKPLTLFGEDALLDWPGAFEALIPERDAFMAKAIAAVATGKPGVAPAYVFDEIDGKAVWRYAMPEGGPQRSAPQGLGDGALLGLRGVSAVVGVVGSAHARGMLREWPEALQNHDVSSLLADAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ16</accession>
    <name>A0ABQ5RQ16_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA-processing protein EFG1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">rRNA-processing protein efg1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_001555</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EFG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019310">
        <property type="entry name" value="Efg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050786">
        <property type="entry name" value="EFG1_rRNA-proc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911:SF1">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10153">
        <property type="entry name" value="Efg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="256"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="481"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI59630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="515" mass="54870" checksum="F86C1FAE7BC44DCE" modified="2025-10-08" version="1" fragment="single">MGQPGGFGGRGQGRGGGRGRGKLGPRGGGILKKHAGGGGGSASRGNTTTSSNKHRSLKNQIRGVERLLKREGLDSRVRAAKEAELAELQEKMAKHQQQDLERKYAVKYHKIKFFERVKLERAIRQLERRLGETGAGGKSRGSEEEEEEEEGQPLDLQAVKAKLAALKDDLMYVKHFPKGEKYVSILRDVEDPEAQKNLEAERERLRAIMRRQVAEVAAVTEADEGRALLGDKSLAAAAASHTDGNLKAKTKATDVGIIKAGESKPPQKQQQPKRQHQARLTTTIAANMDEEDEYRDEEPQEEVSEQEGTEDDFFLSADNVDGGGDVGSDRGVLDIDGGGDNDSDQGSGSAGGDVEDAGQEEEGALGVRRVKPASGGAADRGAAKRVPHPGASAAMMAPPAAKRARVEGALLQREAIKQRRQDGRSQTEQRAGAERPRHAFAEKLGGSGDGDEDEEADDFFLSADSDAVAAGVVVSAGEGYAGNGIRQASKPHAKGPVGWREKGRDRESGGGEEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQ50</accession>
    <name>A0ABQ5RQ50_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="223"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="373"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="895"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1252"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1329"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1389"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1526"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="601"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="785"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1149"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="244"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="406"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1253"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1346"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1412"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1564" mass="168524" checksum="90D3CA5CC06DCE77" modified="2025-10-08" version="1" fragment="single">EARARARVIEEAAARAPSEAEVAALRARLVEVEHHAENRCIAVQELERQLQEARRQADSEVATLRTRLAEEMRQCAEGRATVQELERHLQEVRRQADGAHSRAQQLQGVFTVLAQLGLPAHTLETVATAAAITTAAPAAAPSLHMPALLPSSLLRSWLSSSVASALTPIQGAVGSPSTATAVAAAAAATAAAAAVTAGQSAAALTPAIISSLREQLAAALLPQQQERCPQPPSPQPQLQRHPQGANSPSSLNAKQSVSGEMQALRRLLHAARAEVLLYRHKLEGSSIRDLQRRLQQQRRHSGVDDDLAVARSNRHRRSRSTGLSQGRTRSRQGRGRSADEDMDLRSLALDLDSVLELEMDLDYRRGLDYHQQTLRGGGQHRSRRWYDTDPGSCSYSDNDVEHDSRNPAGRNSSSRNRAGRARRSASCSSTANTDSSVSEPCNSARARHDRRARRAPGSRNPGNASFRAPSAAAGGPALDAMWLRVQQLALEARMARSREARCLEAARRADAVILLLHGAASRAQLLLAGAPRQQDQDLADELADALARAMRHRQHIARLTVAAEAQGLSQRGVEGEGASCAAAGLQDPAQLKEWEHQQAAAANLAGQLSVARDEAAAAEKQAEALRLQLLQQAEAHAAEVVTARNTAESARARASQLASQVVEARAQQQAMQREAQGLRRQLRGFMLQIRHLMDDLRQRTDLDDLNNIAKAVVAATERALGLWTIREHDHVQATRRAVSDLRSEAAECRQLLQSLATAQQQATSQLAASNKAACAEFGKLRARSAQELSAVKQLLEGEVRQLNGEREQLQARATALQNEKELSEVVCSSLRQQLEKERHAVEEEHRARVEAEERVQATEERLQAAMAHAVHVAEAQRDAVQAAAIAAAERAISAADEREKEEAKRRAEMEARLVEQQERDSSRLQDQQRRHEAALQELKDAHEAETRETAAQLDTASRWAQALQEQLLQRQGQLRQQQGELDGLRTMLHDAEQQLQDVLRELHQAQQRAEAAEARETETLQRLVEAEVATAEAQDEAARHLVEVRVQMNAVAEAQAARNEAEIARDEVGRMLMKVREELDAAVESTRAAELQIAKHADEAHAARNAAEAQSAAARLLEVQVEVARDGLRQAEVRALGAESEAHAERSAAAQLRQQLMDARAALADAQEAARMAAQELVQTKARSDELQQEMLAAERRLEVARAATRLHEQQLCDEQQESDRLRCQLRVCQTRIFELQQASAAVGIDFGRQRDSSGGAATGATAGAASGAGRGPMSGAEGERYGARRGCSVGSEPVGASHRACQPVWAGERQAAPNVDAQQLPVNAIPGREEETASGSATGSGWGSFPTSKASTPVTSGGGETPPQPTDVAAVHDDGLRKTDTVAVATVDTIRPAPPLPHHAVRNPAPAAAPLTSNRPAEQGTTAAGTDSSAVTQPCVAQICSTANVSGSCTSASQTASALPTAPSALHRRALLSHQQATSPQQHVEESLGDQAHKEAQGLLDEVARHCQRLPAINQVLDVMLLSDSGDGDNPAPGHAWQPDTDKIPDGGCGNLNMDADGFLRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQH8</accession>
    <name>A0ABQ5RQH8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Membrane-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_001788</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046262813" description="Membrane-associated protein" evidence="3">
        <location>
            <begin position="29"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="860"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="959"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="989"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1005"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI59820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1047" mass="106869" checksum="FADE06D1B5B025E9" modified="2025-10-08" version="1">MRKACYPGIKMIKLLILGFLSSMAIVDAVASGTYPIVPSTPPPSYPLVPPPPNPYHPSPSTSLPPSPASMPTVPPPSPPQPSPPSSTNASSPPLVYPLPPNVSPVSSPPVSSPPVVPSNSPVTPPSWAPVYPPSYFQPASIQPPPAPPVADTKVPLSGYVLFVGPAASCSGIAGAGSTTANVTTNSRGELSGEGAIGEAQEGLMVVVQPAGSSCKDAFTGLPLPFAVGAPWTTPASGTAQVTVTPATRLLAYTNLTDAANTTGVIPAAYRLFGVDASGAESGALAALKQPTAYERLTGVRMLAVDTALTSLVVTAVPAVAAATSTSTACSSAELRIDAIFDALAAKAATGLNLTSPSLCPAVIQATVAACNGTNANTEDASLKACRVYDKAVATLVEMNAEEQAIIPLAAVGLMVRVSYVVQAVGVAAVTSAINGNVSAVDDLMAGWNAHLATAPINLTAILLALGLGDAPSAASPSIFVRGSGSLTNCQVSFNQIVDPVNTKNSTTNAKGVAIFTNVTSGLVTVPSGCRDAALSVSSKVARSPFSMNALVPQSLIGGPIFLSPAAYLATAAFLVRQRHEAPRGITAADYDAVYNYFGIGVAGAADALPQGADFVRQNWPDSSALVVRSYLLNQRLLGAVGPSSSFMSGLLKHEVSTDDVSQSFVEAMSYDMVNKTLKPDDKDYLIGLMRRYYQSRIQASSSSGRRRLLQTLDLTQLNQLLASVASAVAEVSNQLLNLDHQVQVAVAAGQTVDVGALLLTAAKVSSVAQSALTNMLTELAAAASSGNTTALTALTSQLSTSFSTAGLQSLVQSATVDETGIGAVGNSDGTPTVEPSGTAPPVLPAALPPSTEKKTVNTGAVVGGVIGGVVIGAMIVVIVAAVLIRSRRVKQQVHGRRDDVRINVMVPDPETGAATNQALLGLAPPPTLVGGAAGNRTALVDNKLPGATNRSLGGANAMAENVEDQEESDRSETMVVPWASPRITDSRISRGTPTSTTSPGGRTSHFRDNPVHPDPKSASPAGRASSPRDPDSSCLNDQPGWWTAANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQN8</accession>
    <name>A0ABQ5RQN8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_001190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="206" mass="23264" checksum="37756484C163C841" modified="2025-10-08" version="1" fragment="single">RDLLCVQFMFQRTYQGYDVGKLSLGDFCHPDNPDRADWRLPLPPPQEWPPGATYRLCVRERGTKTSRVQRAPAIYLQSNPTCPAQCFPRTLALYMWPLVSQTQRVSRPEPPLKGTTLRQSKIHNQEGDIVSDNGVVKQRNVRGCYPAIDPREDLVTQGRVLNAHKPLRCDRSRKSVQRQHASGAFTVLAIAGRAGQSAEGKRVGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR09</accession>
    <name>A0ABQ5RR09_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Membrane-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI60003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="37858" checksum="F741BA9A65D34877" modified="2025-10-08" version="1">MPNFSSSEILVLVTSFSMSAVLHAAAVPWDSHGYVRQPSRAMTSRTCFLISLSSLLLASGVSLNITSQIIVAGSRLALCGYYTISYEMWAQSTAAYLYVTDTTCNSLTASPCQPPITAALLPLSCVDRLTCKGGTSQPSAIPLCLVVANKNATAPLEVNLVVSGSFTKVNSSSSSSSSSSGSSSKGFSTFEVILIVVGSAAGLVVLVGLMSLTSWLMCFKPPISRQIWGQEGDGDFGAKPASVVGAPQRVRSAPAPPLLPSPATVYPYPLNGGAPYGIALPLSPVPYEMAPMPLLPYVSMRSPSALAPLPTEAYSGLSQPPSSPPPLLRLPPRLPPPPPTTAQSPPPAATQSQPPLMLGNLEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR50</accession>
    <name>A0ABQ5RR50_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heat shock protein 70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="12336" checksum="6C4B6336F67B584E" modified="2025-10-08" version="1" fragment="single">SVRAAMAQRSKAKIRHSAAAAPILICVAAAVAAVFTSAASVSAASASRDAKRVGPVIGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTDTERLVGDAAKNQATINPKRTIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RR65</accession>
    <name>A0ABQ5RR65_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001674</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="745"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="877"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="643"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="890"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="949" mass="99422" checksum="239D03789B5EA98D" modified="2025-10-08" version="1" fragment="single">RINRGSGGGRGCLRGHGQRPAPTCTSHRPSVRSMPNGFAPARRQPHNANEVANSLVKKSADAKVAATRRRTLQTTAVQRQRHGIFAAAGKDGGSGGSGGGGDVDYRYGDNKYEYGGDGDDAHAGRRSTSITALYKAALTAASAAADDILATATKSGTGAAIATASDVSSGIYVNAEKPLTGSTASSSSTLPYRSYTTDKLGNDVPIRTRTVDPENKFVDIYPIYMDGDAGSIAEVLQNDDGSLWLEFGESSAESQMHGKSGVNVGGDSTSKNPRRQQQLQQGPLEKETEMEYILYDVLGDKSVFDEGPDSVAATRHDGHLPVTTDTFPYDIADFFLQQMYEYLYDYTSDGTTTTGVNPRRLTRGSATAAGTVDKYRENDEEKAASLELWQALYDMFSEDDATFGPQQEAMYIRLYEQDPVLFWAVHDEVVARRVGDKGEVGPGRSDPAANPAAANPAAAATTAVIAVANGGGMVIDTGSGSSAATADKVVLWDATSEPGRGNAAYEYPSRYGDDVYESEYDQMLWGKEHGNHLLQGNGVRQQLNEKEHQQQQKQEQDVMWKAADSSTAVAATAAAAAAAASAGPSATSAAGDRKRETVVAVNSIGDESSTAAAVTAVGAFAARDVSSVTADPAAMGNDGASLTPTTPTTTTTTTVTPVTAHGSEQAVAAAASHLVSLQAMSSWSVRRMQPGGAVRPLSTVPEVLSGAVDESAAVTAKDAVITNAPGSRSATRDAALGVLKRAGTIILFAAVAVTITALFTSLVFTSLVAVAAFKYHRGRLAYAHAAIPNQRNTSRSSAAAPTVMIRFVYFRGARSGARSDMENLSSLFSGGDSGGDNGCGHGALWHRGSSNTIAASAYWDRDIATRADNRRVHGNVEEGWAEQHQCSGSDSGGGGDGGGAAASDTPSQLQLLIPPLHDQPRRASGGGLPLHDSSESWISQIPIDRLPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRZ3</accession>
    <name>A0ABQ5RRZ3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002462</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="667"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="672"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1248" mass="131348" checksum="1481962DA2C81758" modified="2025-10-08" version="1">MAHHGSLRTYDSRVASGAAARACIGECVAAGLLSLISLMSYQQLAAPHCAGQPHTLPPDSAVLVGYPCDGSVLTRMFYILKHSPMVLWDICRVKFEDVWAATGLLMIYVRFLAAILIPAPIYDQVVAPVTVYYARFVNTAFHVLQGPRYKPSYLGLQSLFTKHAPFDVAFFLSLLGVWRVNMTLELAYCAWAAAATAILWYWYRDFIPGVSLQGQMLTRLLVTLTNIYLDRLLSRHHHHRGPTGLGRGSGPAARTTAATATAAAATAAGGSAISGKDAAAAAIAVKEGAECVGGEEKNAEDAVLKTLFPSGDNSSSSAEAVNCEDRILPPSGPVFLRRPKTPAAAPMMNSSISSRGGGNAGSDGNGNSNAAGNGGSSCTTRTAPSGKEETTSTCWRTPPSGVAPASRNVTAALATACDGAEDVASRSQADGTIAAPVPRIAYCSPYRRHAVLVKIPWAEPEQITPRFEERMGALVSEQGLKLTGVYVRRGCVELLMVVEEDMDRSPLPPLPPPPPLLALPTATGPQSGNTGTWHLDPAALLQALGLAPRESETEWQGPTNRDGGGGCGDGSNSVSIGDCSCGAWTAQQQQLLRSVTQEDSALLTREAAMRRAPEEDVGKKTDYVCVTGLESRVVLMTSSPTPVAAAASGGLPTLRLSVALSRRLLAKQREQQEEEEEEEEEEEETGKVVGQEEMGEAEVLVRTANTHLPVRVTIRKVRCRSGGSGSASVSSKWLFGQEYEYGYEYELDIELLDVPPRPGVVLLEFRYRQWTPTPKATASTATAVGVDQLPIPTTPWLRVTLPVLATCDTAVSEELTAVVSSWPAGNVHELDELLYDIGACTAAAAALHADSDGDVTMALSLHLLSYAESTGLRSTAANIQEMMRRRVTSGVVVGCGGSVPLALRDPVPAAVAEAGDAESSETGSFRGTARRLFGGSCRGEERGGDDDGDGGCKGNAAAAASLAHGDADGGCSQIATGSSSGRASSWRLALAGTLGLRPCSPRETAAFQAFADAWADGLGHISGPRDDQHPLGPEHIVVLVGSGMGSVTTLAWLALPHRAWLRLLRGSKVPRLLGFCASKALILAGLRPPSAASAYAGGPAMLLLLEGFILPLACLVSPRTAFFISCLKLPLDYQSAFMVGAASYTLSAFKIAAAVELMSLTVTLTCHLYLRCRFDGQLGRAAGSSGGFGSGYGGPHGELVGGKTGRRSCSDETRLMRMQKMRKARVACIPISSTAGSGKGPMLVMDRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS60</accession>
    <name>A0ABQ5RS60_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_002575</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF18">
        <property type="entry name" value="OUTER ENVELOPE PORE PROTEIN 16-3, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI60423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="27222" checksum="705A465445D21B1A" modified="2025-10-08" version="1">MSSEQCVVKAAPLSNNVAFITRTSDDAPNSSVRGAQVPGYAAEGEAAIADQPCTLKGLSGGLAAGVLGFVFGFVPSMFRHRSLGMLHVWGAEGMASAKAFAVMSGIYTTVQCLCERIRQQDDGINRLIAGGASGVAIAWRSGLVGALQSGVLLAVVSWIFDFGSGSVSPAKASTIGCSSGACRVSVGRLVSCSPSSGEVTDDRAGQHWQRRAPFHIHLGRGVAPWSPGSLPPARVLSTPVVMWMGPIINQGYFSQPTHSLYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS73</accession>
    <name>A0ABQ5RS73_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045436360" evidence="1">
        <location>
            <begin position="20"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="201" mass="21823" checksum="E9686F4AC157FF97" modified="2025-10-08" version="1">MQRQTFLAFILFALSAVAAEIAEPLALIGFHEGARRNTECKNGFLTGIEVGFIEDAWAFGEPFIGSIRLLCSNKTLSVEYSGDTADTFVFGRLDLKTIRKAECEGNLGVAGFKWRSAEIKNPEGAEARQAVLVVLCNRPDNAPAEVVLSHSPESGSRSAASHHFSCKQTAKYAGLPAPKISKVVIWYNRKSFAGLEPLECA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSC6</accession>
    <name>A0ABQ5RSC6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002673</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058210">
        <property type="entry name" value="SACS/Nov_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052972">
        <property type="entry name" value="Sacsin_chaperone_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047352">
        <property type="entry name" value="P_loop_sacsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15600">
        <property type="entry name" value="SACSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15600:SF42">
        <property type="entry name" value="SACSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25794">
        <property type="entry name" value="SACS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="4490"/>
            <end position="4526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="952"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1007"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1112"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1245"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1463"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1857"/>
            <end position="1887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2671"/>
            <end position="2699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2906"/>
            <end position="2932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="3217"/>
            <end position="3245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="4374"/>
            <end position="4394"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="4446"/>
            <end position="4487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="953"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="968"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1112"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1136"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1857"/>
            <end position="1876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2906"/>
            <end position="2921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="2922"/>
            <end position="2932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="4538" mass="466336" checksum="BBCFFF60B4BA37F0" modified="2025-10-08" version="1">[encoded data, 4538 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSF2</accession>
    <name>A0ABQ5RSF2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA 3'-terminal-phosphate cyclase (ATP)</fullName>
            <ecNumber evidence="2">6.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002395</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.4" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52913">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, RPTC, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="6">
        <location>
            <begin position="9"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="7">
        <location>
            <begin position="200"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="364" mass="38202" checksum="A9E7AFB63F1ADBFA" modified="2025-10-08" version="1" fragment="single">MVTIDGCMLEGGGQILRNASALAAITGIPLAVDQIRAKRTRPGLQPQHLTGLKLVEALCGGKLEGGEVGSRRIVLRPQQSPRTGNYNADTRTAGSCTLMLQAAMPVCVFAGPELSPEPAGDPGFSQLELKGGTDADMAPPVGYLTEVLIPLLRNQYGDLLDGLDVQLVRRGFYPRGGGIVKVRVPSLPAGTSLPPLDLTSRGNVTQVTIKAFSAGRVAASVAKRLAASAEAVVRRTLKGLGPMGRGVPVLVEVVYETPDRAVGDGCGVLVFADTDTGCRLGASAKGERGVPAEAIGERAAAELTEALTNGTCVDQWMQDQLIIWMALGSGTSRIRCTEPTLHTRTAMVVAEQVLPGVKFRLHRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSJ9</accession>
    <name>A0ABQ5RSJ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_002407</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN GUN1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="249"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="324"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="799"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="849"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="878" mass="90078" checksum="BDFE74EAF8B6B15A" modified="2025-10-08" version="1">MDPAQPNGDEMAAIYFRAEDLARRTVEYHRVRETEHRARERFGRRFRPFPWSEAVAAVLSDEALEDEASVVTLIRELGRQGCSEVALAALDLVVSRGSPPPSKAMFDAALLVCATEYQDQRCLEQFGRMRSLGLSADLLTTNLVLTGLGCRGRLEAAVEVMTEMRNKMGIRPNSYSVLMVLQACNYKRRGAYREAIDAVQALEAGGRTANEEVIEALLQVCESAMHQAPSFEAALAVFSALSELHLADCTRIYNGLLGAAGRAGRWREAQALYAQMQDDDVPASLETHTALIQACVVGRALDRALHIFEHLVAGRSAHETVPASIATYNHLIHACHQAGMLEKALEIASWVQKTGVEFDDETYGELMATIDVAQLWDDKAMKQARQEHKVVLPRNLRPAPYDAMRVMYLDHLEILQQEELLALEKLGGLGSWASKSLTRGAAGGPNSGMLPPTPTASLSLTSSARSLARSVVVGAPSGGHDIEPGYGHGYGAAAGRNVGGDVPSPSGSFQPTCSPNVAVTSQGPWFGQGTCGGPAAAAAAAAGERSGGSSLAVSPSLVVGAPAAAGAAAQVGGCVGSIGASQSEVQSRAASVKQLLKQLSTRPLPVLGSGGQGSVGGVSFRSALGSCGTAAPPPAAASTGYHDPPVWAQIQTMDAVTAGPGGTPYSAVVAAAQAAGAAGGTDGATESGGVPMALTLAIEPANLYGQSSAASLGSSHNRTSLRSMVTNRGIGGGSTSGPGNGIRIVLPTTSFSAAASLASASAVPSSTLPPLNDPIAPKPGLEVTSAVAAAVGAQSVMLGTANSPRTGPGPATASPPPPGRHRSRAAWGQPGPPDGGMAAMALAANADFVPSPLPSLSPGPVSATAGGTSRKYVSVLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSM9</accession>
    <name>A0ABQ5RSM9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="567"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="611"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="695"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="711"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="778"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="815"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="842"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="861"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="875"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="954"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1074" mass="105998" checksum="CB0023451F7EF210" modified="2025-10-08" version="1">MRAGGSPPALQPSGPPAPQPALQPSSPPALQPSGPPASPPALQPSGPPALQPALQPSSPPALQPSSQPSSPQALQPSSPPALQPSSQPSSPPALRPLNQPSSPPALQPSSQPSSPPALQPALQPPSQPSSPPARPPALQPSSPPAPQPALQPSSQPSSPRASPPALQPALQPSSPPALRPPIQPSSPPALQPSSPPASPPAPQPALQPSSQPSSPPALQPSGPPASPPALQPSSPPALQPSSPPALRPPSQPSSPPASSPPALQPSSPPALQPSSQPSSPPALRPPNQPSSPPALQPSSQPSSPPALQPALQPPSQPSSPPARPPALQPSSPPAPQPALQPSSQPSSPRASPPALQPALQPSSPPALRPPIQPSSPPALQPSSPPASPPAPQPALQPSSQPSSPPALQPSGPPASPPALQPSSPPALQPSSPPALRPPSQPSSPPALQPSSPPALQPFSPPALRPSGPPASPPALQPSSPSASPPALQPCGHPASPPALQSSSPPASPPALQPSSPPALQPFSPPALRPPSQPSSPPALQPSSPPALQPSGPPAPQPALQPSSFQPSSPPALQPSSPPALQPALQPSGPPAPQPALQPSSPPALQPALQPSSPPASPPAPQPALQPSSPPSSPPALQPSGPPASPPALQPALQPPSQPSSPPASPPALQPSSPPAPHPALQPSSPPALQPSSQPSSPPASPPALQPALQPSSPPALRPPSQPSSPPALQPSSPPALQPSGPPAPQPALQPSSPPALQPSSPPALQPSSPPALWPPSQPSSSPALQPFSQPSSHPALRPSSQPSSPSVLQPSSQPSSPPALQPSSPPALQPSSPPALWPPSQPSSSPALQPFSQPSSPPALRPSSQPSSPSVLQPSSPPALQPALQPPSQPSSRPASPPALQPSSPPAPQPALQPSSPPALQPSSPPALRPSGPPASPPALQPSSPPALRTPSQPSSPPALQPSGPPASPPALHASVILEGSVTTAASPPTITATATAAAATATTSVAAARCIATVDETAAATAASVSYEHLRRTPFPYLTVHPPRTHFIASLPWQADEVVVYGGDVRPRYAVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTG4</accession>
    <name>A0ABQ5RTG4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002942</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052523">
        <property type="entry name" value="Trichothecene_AcTrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791">
        <property type="entry name" value="GNAT FAMILY ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42791:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="153"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="443" mass="49177" checksum="EDED750BCD5D4859" modified="2025-10-08" version="1">MQLSHTFIGRQVLYNANLKRSGCGVSSKRAFTAQGTATSLFVEHPFKARVLHIARGAGSAHDVEPVVGVASSEYPASVGDAAFRDSAERRQNPLITVLSYRERPDIARAAIENIAAAFKDDPSTTYFCRTDQRVQFYRSVLSCLLDWYPDEKQLVCTMPPDAAAVVYVYPRVRELGSWAKLLRGGLAPLACVRLDRLVAGMDASDFFAEQRENFIKSHGPFMYVSVMAVRPDRQGRGLGSALLAFICDRADAAGLSCYIEATSMRSRALYELFGFQLIQEWRANPEMPTTYVLSRPPRLYEAECIVADTDSALSTNVVAQQPWWQHGEQRRQRFAERRSQQRQRRQQLMHQQHQTHQPADSAGSDSDLLLYTELDGQSWWPLSPLEPGSNAGTVETRGVELAPAAEGDVTQTSYVDMVNCERAAADTGADTRSNAGHPAEFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTH6</accession>
    <name>A0ABQ5RTH6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_003100</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03273">
        <property type="entry name" value="ABC_SMC2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027120">
        <property type="entry name" value="Smc2_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="14">
        <location>
            <begin position="516"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1174"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="267"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="669"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="754"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="955"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1174"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1187"/>
            <end position="1196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI60880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1203" mass="131648" checksum="9FA92F5387E38EF1" modified="2025-10-08" version="1">MYISEVCIEGFKSYANRVTLSNFDQCFNAITGLNGSGKSNILDSICFVLGIRNLSQVRATSLQELVYKQGQAGITKATVSITFRNDDPKKAPTGFEDKETISITRQVVIGGRNKYLINGQAATETRVADLFQSVQLNVNNPTFLIMQGRITKVLNMKPPEILALLEEASGTKMYEKKKQNALRTLEKKQSRLSEIDHLLKNQLESSLLDLRKKCEAYNEFTSLQSARDRLLRFCLAYDHVSCTRIVGTGGDEIATLEAAITAKAAEREERQQELADLGAQARDLATEKEIKMGGEMRELKKTVDELQLKLSRSTTALKHKQDALKAEKALLKQLQAQAAELAAQDLDGAVAAAEKRRDEAKADLEAIEKAIEAATRELAGAEAGDGRDESNRSLQERLTDAQLQQTAAEAELKEAELAVKHLTKQLADQRKAAAAKEKEGASLARELERHKTKVEDCNAQLQGLSFDEAGMKALEARRDQSQAVVQHTQDVVKGLEGEVSVIRFDYTPPHRNFDVRKVHGPVAMLVRVNDPAAALALEVAAGGKLYQVVVDDDTTAKELLQKGQLKRRTTIIPLNKVGYPQMSSAVLEAAQRLSGGKARPAVDFLEYDQHVAPAVHYAFGNVFICPDGGTAKRLAFSREVNMRCVSLEGDDFNPAGTLTGGSRGNRACLLAKLRELQSAKASLAQYKTELAQLEKQLGEMEAIAREHAKYSRELELAQHSLGLAQQRVAGSEAAQLVAVADATEAQLTAAREAGAAAAQRKKDLIEQAKELEREIRDFQKDHGARVKAAQDKLKKAKTAAEAARKALRAAELAGVGARAEREATGGEMESLKKQIDAALQSITGADAEVTKLSEEVNAAKEAANAATRRLQAMQARLSECDGEIKTLEASRDAMSKKVSECDQEIKKLDAKIKSKRDTIAKSKEWIGRMEAEHPWIASEKRHFGTGDYKFEGVDINAMRKDFENLRERCEALQGKVQASVVSKLQKNEEDYQALVSKRTVVESDKNKLEQVIEELDNQSRAALEDVWGRVNTWFGQIFSTLLPGTSAKLEPPEGATYLDGLEVRVAFGSVWKESLTELSGGQRSLLALSLILALCRFKPAPIYILDEVDAALDLNHTQNIGRMIRENFPESQFIVVSLKEGMFSNANVLFRTKFVEGVSTVTRTVTETGRAVAGGPGVRENGGGAKAAGAGRSGRAVLSNANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTI7</accession>
    <name>A0ABQ5RTI7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="165" mass="16953" checksum="B3508C78ACD22598" modified="2025-10-08" version="1" fragment="single">KQDVQCTIPYSQGSGGVVVPELGVDRSDDVAAIQSQQSLLRRHLGVALVSLAAGPNLRSPAVKTSAPVTPLLQPLNGMSGSELEVSERLDEQQHVLIQRGHVEVAGLPAGVYRLTIARRGLRALLLVYDSPVVGPADTPSAVPPPVEATDASREGAGGVTAGGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTJ0</accession>
    <name>A0ABQ5RTJ0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_002785</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="SAP" evidence="6">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="465"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="689"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="45"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="283"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="378"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="539"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="766"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="914"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="993"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="381"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="579"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="779"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="918"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="930"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="946"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="963"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="996"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1071"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1095"/>
            <end position="1114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1266" mass="130369" checksum="F1EDCC8D45AE1944" modified="2025-10-08" version="1">MEPTKPFEKMTVAELKAELKARGLSEKGVKAELLARLQEAEAALEQEPAAVAADTAMDGQPDPAISTGQPEPQDTLKAEPVDFEADDKEQPTTEAEAQEEQAEEQDADAGQAGADTVEPDSIEAPTEAEQVAPEPEEPVEQEIDKVLADTQLDPSDAPQAAAEPAKEPDYGPEEWETMEIPEEYRPLPVLRVRNIKQEAKPEDVKKVFEDAGIPVHSVDFDNVERNKNVALLRLQPPPLSWKLSKELGPNPLDEALKKYIERKEQEAKAAAIAQKAAEAAAAGAAAEELVATTGSAEAKEEATEVEASSATAGEKTNGAAVVPIAATEDASAADIAEPALNAEAAVEHNAVVVEAGGEDPGKTEAAAAEMDISAVAGDEGQDARPPADADRKPAATSGGSQPAASTPSRHVSSSHAPVTLHVEDGRLDGDVLKVARYTAMRLNALQYPLTLFGDRLMIDAPSLQCTLYLGNVTQDNDEQLKVDMAALGRVIRCFIMRGASGSSKGYAFVEYATPAEALKAKESLEAQSRKAFQDMMATKNDLQRQRGRPATSALKPATPVEEGKEAQGDQGGEAVVSSDKLEGEKVAGLEDGKGEPDTQMKDQDEAAAVEGAETGSKERAPEEEAAGMIKLNVESDPSADQEEQPKVVEGSDEGANDTKDLQSKQPSHTSKIMRAELTHIRRAQDLFSRNLYLASLPMSLSDEGVLRKVFGEFGPVIAASITRGPQGYSKGFGFIEFLRSDHAAAAQAALDGKEVPTLGKLAISFVNPSKSTDRRAGPQGPGGRGGPQGLGGRYGSTGGPGRGGMMTPGFRAGPHNARNAPYFAGPGRSGYMQPPPPPPYGISPMMRNGPGPFNGHGRGGMLGPGRGYGGGYGIGGMGMGGGYGGYGGGMMGRNETGGGGYGGGYGGAYGGYGGSRGGQQQQSSSQGPRSGMGYGAGVGAGGNSGTAGTASPGYGSSSYSAGNTAQQQQQAIASPTASAGTYQGAGYSQGTGTYGGQASQSQYASQSTGYGSQVTGYGSQSGYGSQTSGYANQSSTYGGQQQNAQAASGYSAQQQQQQSTYGNQQAAAGTYPTSYSAPAQTYGANGYSGTAAQVPSSTYTAPSTTAGYGAQQSTTGYGTQQQQAATTVYPGYSSAAQAYGATGAAAQQQTGYGAATQTNSGYGQQAQQQGSYGAGASYGASAGAVYTGYGQAAGYGASTQQASGAYGGTGVSYGQQAQQAYGTYGQQTSTYSAQAYGQQQVGQKRDGDAYASYGAGYGADYKRPRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTS8</accession>
    <name>A0ABQ5RTS8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35830">
        <property type="entry name" value="OS05G0299200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35830:SF1">
        <property type="entry name" value="OS05G0299200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="536"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="591" mass="63628" checksum="A56B83A15D8EA005" modified="2025-10-08" version="1">MPATIQLLTQRSKCCHSTVRRRKNSWVVRASHNPDHIYDLPSASGRAANSVELRLHASLSQTQISHLGKASKLLAVTAVAYHIAFSCNAARAEDRPWKPRRHLRRMGERFTDSWAEDLVEERESAREKRQEDVRLERLRLAELREADHRRIREEEAALKRRAQEIMIREYDAARAGGLPFKLMFRGTDFAPLVVWSGILAALVAAYRSRFLTWLTAGPGRRTAGGKWVYDRSLGGRKVWVPDNSSFAGPTSATSLSEKAGRSLKDEEFDRLASFAAAARTAPGSKTTTTSSSSSSATFSLPSWWDPPPVLYASASKKAARAEEAARLLNRLESAKVAGRDYELDDIRALRRACQDGHVSVKPKTVGGRDAIYRTAVEAAVEAAQSRNSDTLDGVSPPRWVAGVAKDLNMEEQRAVGIVAGVAASAVRARLLDAVSCLRSGNTSEAVYSLARMASLLELLPVLQPGAVQVEAVASSLRDRSSVPERQMLFHLLAQLCEEGAEGAAELLGFDPALVLAEQRVALDAARQERRREEGLGIAAAPAAAGAKTATEGAGRSQTGSGGVNGGGARTDRTSGNAGDGQTGIGPGPAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU24</accession>
    <name>A0ABQ5RU24_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="656"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="853"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="959"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1187"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1237"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="346"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="450"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="656"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="863"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="968"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1188"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1268"/>
            <end position="1277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1351" mass="139218" checksum="C4501306CA4957A8" modified="2025-10-08" version="1">MPVKGYLGGPHDGEPRSATLELLSSGLSTTSGGDASGDIKCSSLSIPGCGCFYSCTCHCCCGEIPYMLNGTTTASDECSSGITCCSCGRRHRARRNGFLSIRRRRNAFLSIRRRRNGILATEEGQEGSWDGTAAYAALGATGKRPTSSFGNPTPESLDGAGGPGCCCCCTCCCRGGGLSDGGEDEDGTKLVSGDTTPLGPAREPCCDPQILSGGVTMSSEHGGGYSPTMFGGSAGTRPPAVQRQPTSQQLLPVLHNNTQQGVLATAIAVECNVTPNSHVTMAKKHTQQMQPQQLQLQLPQFPGAGTGDGGIKGQMLAACERVQFPRREHSPLQTPREPSLPLLELQQQRQQQQQQQQQQQQQQQQQQQQPLQQNQEQQRPGRQDSWHPVEPGICETVPLSATPTNAVVDLTSRSDVELQGTCVIPGSCVSSPVVRPHPPVGPCTSGRSLPQLQQPQPQRQLHQPSPSPRPQQQQQQQQQEQQQQHQPGRASQVCQLGEGTGSAAVDAVVPSRGRHLKVVLLRSADVDNGGAVGSGRVELGQSEWPSPSPCSPRPPSQDQQEGKAAAAEGEEEEGEGKNAPCTQGAVRQPAGAATAAVATAAIASNSVRSAVGDAAAATTCSSRASGALRPGASAWVSGALPGFAGKTHTPPLLQLPSTSASMGTSPGQATGPPVPQESTSNRAATMVINPAGSSASPSYTLGYGKKLMPSLSQCEHLSYVESGSSTAAATTGMNGTPCRSPAPRTVATTMVGAAALGSMPYRVPPRRARSLATLAAVDRIAPRTNHLRQLLVTVQQVTPGSLLPLLPSPIHSVAVDWSSGDTAYTGTHSPKVRPRRSVSTQMPAGYNTAMEAAAGSAVPPPHSQQHPQQQPHVSGVRLQSSPLPGVAGISNLVSGLPVRGWVGSGPPSRRPAHWSTTLASALSDVEDGTEGDEDEDTPNLGAIDVCDGISVLAGMYDSHPEQHVQPRQAPPSPSLPPPLSQQQQQQQVAIGTGLAAALPLKSVARALSGPDSINGYGCFSTTANGLMGPTAAGGSPLCPPDTLVVAATATATGVVAPLLPAKPVSPSMPPALLPPTGALQRRPLSNDSAAGTAAVPVASLTALVSRPSLVSGSRDSSSCGLAAPCTQQDSALTGCKWLPSSLQHTSWSVRRGHDSGSGGSGRVLGILTQPVVGSATTASAIGMQNAYTTTTMPPTPQQQQAPQQHLRVAPSPPSQRDSMAVLGVRLSRMVQPLRQSMWASGGGEGRGPPPPPLSVADAPLRSDDGDGGGDARGGGGDVAARALSLSASSYGSERAVDQRRAGSGQAAAVAMTAPLSSTVLTPRRELHSSSISLPRIWTTSSPASPYDPGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV31</accession>
    <name>A0ABQ5RV31_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Glucose-1-phosphate adenylyltransferase</fullName>
            <ecNumber evidence="3 9">2.7.7.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ADP-glucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_003345</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">This protein plays a role in synthesis of starch. It catalyzes the synthesis of the activated glycosyl donor, ADP-glucose from Glc-1-P and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>alpha-D-glucose 1-phosphate + ATP + H(+) = ADP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12120"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Plastid</location>
            <location evidence="9">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.27" evidence="3 9"/>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI61078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02508">
        <property type="entry name" value="ADP_Glucose_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04651">
        <property type="entry name" value="LbH_G1P_AT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011831">
        <property type="entry name" value="ADP-Glc_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005836">
        <property type="entry name" value="ADP_Glu_pyroP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02091">
        <property type="entry name" value="glgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002772">
        <property type="entry name" value="PRK02862.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523:SF12">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE ADENYLYLTRANSFERASE LARGE SUBUNIT 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE ADENYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25247">
        <property type="entry name" value="LbH_GLGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00808">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00809">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00810">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="11">
        <location>
            <begin position="81"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI61078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="512" mass="55908" checksum="6D8025B67591561C" modified="2025-10-08" version="1">MAALHAQRRASIASQRSDRKSIPSFSSEALRGMRLVRPASSHVAPVTVARSRRAPQVKAVIEAPQLRYEPATKARTNTVLSIILGGGAGTRLFPLTKQRAKPAVPIGGAYRLIDVPMSNCINSGLSKIYILTQFNSTSLNRHLARAYNMGSGVRFGGDGFVEVLAATQTPTDKEWFQGTADAVRQYSWLLEDTKNRAIEDVLILSGDHLYRMDYMKFVNYHRETNADVTIGCIAYGSDRAKEFGLMKIDDKRRVMSFAEKPKTAEALEAMKVDTTVLGLTSDEAAEKPYIASMGIYVFKKSVLVQLLNETYAKANDFGGEIIPEAAKDHNVVAYPFYGYWEDIGTIKSFFEENLKLCRHPATFEFYDPQSPIYTSPRVLPPATVRNCKVSDAIIAQGSFVADSSINNAVIGIRSIIGSGCTIQDALIMGADYYESDEERAALLASGEVPVGIGANSIISNAIIDKNARVGKNVKIINKEGISEGTREAEGIYIRSGIVVIDKGAKVPDNATI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVM6</accession>
    <name>A0ABQ5RVM6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BACK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_004051</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF489">
        <property type="entry name" value="RING FINGER DOMAIN AND KELCH REPEAT-CONTAINING PROTEIN DDB_G0271372"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BACK" evidence="4">
        <location>
            <begin position="179"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="336"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="437"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="591"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="797"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="441"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="594"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="641"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="827"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="911" mass="96585" checksum="75FB46F6FE7C55DD" modified="2025-10-08" version="1">MEGDAEEYDMEDSGLDNELIRQEAWAICRVRELAKACARLSGRLLPTAVEKAARRIARFASRAGGLPGEPEPLTALEVAALQTALQEATAASVFVHMGEPTHEGVAAVEQLAQQALEGIDLSRPVGGGLVPWLETAGGGGGSEEGISVELLVRVGPERVSALSSAFACVLRRTLTSRNCFSVLLLARRLGCSMLTEEALLACIANFPAAVRADKRHFLMLDDSLLLLITSNSNLQVLEELEVWQAIAEWVQHAWESRRCHLYDLLQAGVRLSHLDTLQLSQLQRHPLLVAEPGPGAQLVSAAYSARILSNVHQSRSRQRGSVGTGVLEAGIHAHPAAHAGPVPSQQQVLQYTRSSLSQPQQPSHHHHQQQQQQQQQPERPKPHAALQQLIGSLQARAAAAKARQQQQLLLQQQQQQQQPQQQPQQQQGTIAIETGKEVQERSGLTGGSSSTALSRSPISPAGATPPVVSAGVANPLISAKQDPETSPRPSVSPTTVPQSLQQQRRPSQGPLSQQTREVQGGASGGSADKRQLASALAQAIAKAAAKQGLPRIQVGAGGQITPGAVQAALSAAQKQIAANPQLQEMLREAKAQIAQPSPQHALRPQQSEHAAPPGGGSPEDQQQQDQHHRLEASSSGAGTKPSLSSPGPLASPASSPSPRVISHGIASQLPLSGSHLALIQPLQQEPGRPAQPQQPMALAPQQLLQIAAAVQKAVRSGQPQPPHVRHQLQQLQAFISRQLAATPPGSSQQPLLLALQSMLAGAATSTDTSPHAGHHAGGLHQPALVRDQPLLQRPQVAERQPRLAQGSSSGDQRELQHQQQEQQEDEGIMHAEGSRVEEQDDAHDQCGGGQGDPDKPHADATPSDGAGGQLARSSGAPSGDGEEEEGVQSGGFGREAAWAETEPEPKRRRQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVV2</accession>
    <name>A0ABQ5RVV2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF1">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="657"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="572"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="895" mass="93262" checksum="F51E66DDB77098FE" modified="2025-10-08" version="1" fragment="single">MASVNRRASSLPARAAGIATGSQNDLVLMQSEFEAKVASSPLQEPRSPPVVATFSPVFDLELGGSCASTPQDRRCRGSRTPQDGVSRRCVADAVGPISLRMFPPATPEAEAIKKVSNRLSQMKAASPAVAGLRGSTGLDRSVSDLQVMWARGKAGGAAASANSGTVLLTPSGERLAELCKPTCDLEQELAQQLRAFRASRITCEPPPYSADLIPARPSSFLGASSSPPEQHPRSMGPYSAGAGSPAGPTPGEIEALASELQSAGFLVQILDGTRLSKDARSCLRTLKHRFLVCLGRVISPVSQVVRRRSISRQDYVCRAPALRSLGLTDAAVNGSSDASGGGCGDGDCGQELEEQQQQSEQEPQSQSQSQLQGGREELLNEPVVVEVRFREQFLIANPTQAYQLLLLALPVVFVGPLRRLDAVVDLMAAEVAAVFKEAKRPLPPWRTKGAMLSKWAPAQLGELGRLMRCAVLPQPHQLFQCQPLDKPVKGEKSSGSAIGVHDRSTAQHAVVAAAAAVAMARDPLSEPLDPWPRLGRSTADGIAMVATAAAGTVVAAAGRDDVTDDESGVAHRSNSAPPRTVAGSRSSVNGPCDGGDGGGAVTADDNGVLTQSESGLKVINLDSLDQLMQKTLASISQVEPEVAAVVVAGRNARPLATAKQKNAMRETPFASPPTEPATDANPQFAAELSFPILPAISPMPDAGPVLTVIRPTGRDGQEAMAVANVVPSQPSPPLLSGAGGAASTAAPPPPPVVPTFQPYRGLSALSPFACVSVPEQYECEHDGQDSPDVESYDCGSGGVKPAPWSARLSNVTGSGDYSSIAYTQPEFTFKHSAFKFADPTRGVNGEDLDLGRRNVPGGSELRQGPCLTLATPTRPPLLFTATDSSAGAIAAAAPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RX50</accession>
    <name>A0ABQ5RX50_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI61974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="251" mass="25905" checksum="76A738B115FF5090" modified="2025-10-08" version="1">MDVSCGISFSIKRVRDSHARNVLWALGGTLAATVIIFASLGSIQNQCRSEDPELLAGITGFSGAVLGCKKLYRYYWFITVLSFVTAAYGAWTTAHGLLASSRGAVLGLIVIANLLTINMTDAFLSIIDVPKFQNGPARDRVHTMIAGCIITAFFQAVSIILVGLEPNAVAPAEKLDAVPKSAPETTLVELASAPQPAPQPIAAPEVPAPAPAAEPAPAAADVEAPVEPAEAPAAPVAAAPEAESAVAVEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXQ4</accession>
    <name>A0ABQ5RXQ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aprataxin C2HE/C2H2/C2HC zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_004792</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI62201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032566">
        <property type="entry name" value="Znf-C2HE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486:SF4">
        <property type="entry name" value="APRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486">
        <property type="entry name" value="APRATAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16278">
        <property type="entry name" value="zf-C2HE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aprataxin C2HE/C2H2/C2HC zinc finger" evidence="2">
        <location>
            <begin position="879"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="579"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="713"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="946" mass="97934" checksum="DE2049E9B79B8D66" modified="2025-10-08" version="1">MAKLLFPEGKLIPVPLGVIKSLGRANVARCCEAVTVSRVQCTVKNIINKDGQHSLKLTSQGSQNPTVVIRTSGQNLDLHQGQSALLGDGASFSLISTRPDLSITVLLDRDPQEATGDDSSQPSAVPGVAEGNANVTMPINDRSETTTEPARSEGDAAQEEPVESGVAEADPVAMRRKVTAATAKADSYSSPSEADASDGGGGSAATAGEDGGSDIEWDAGVLRGATKKSSALPPTGGSTGTGQGRSGGGKGGKAAARGVGNDKPLSPLAPPVLLLLSGLPGSGKSTFSRELLATSPVAWVHVNQDAIRDGRPGTRQQCISAVRTALEDGACCVVDRCHVDTEQRSALRAVALGLGLTAHCVALQTPLEVCVKRVAGRSDHPGGVQGEGSKKVVYMAAGQMKGNNWPPAASEGFASVMDCHNDADAAAAVRAWALYGTNGASETQASGSVTARAMKDVAEGTDDGNRNNENIPRAPVLTVAVPVTHAPPEVMQKMSAAAADVAVAATLAMWREYVASRKPVTTGIMSFLKRARTAGKQGPGGAGAAVAAIPAAKEVASRLIAMNGNEERSEALPGAITARTPANEDSKGAATGKGPVEAGVKGGPGAGIATQSRPRRLRTEAMAVGAGDHSAKRQKVDGAQAGRGSNVDGGGGAAAAAGQAGNRPGSVQMKAKDAAGSAGQGHGQRAGQGGDRPTARNAFAVLLASASRQAADGHECTRGGAGSGDDAGLKQLKHRHGAAKATGDEPDPRFKLSAPWAQALRNTALHPEEAQQDVLYKDDLVVLIRDVFPKAKHHALVVARDPMLRSIADLRKLHLPMLAHMKEVALRWVQEERSKDPITVAFKLGFHSVPSMAQLHMHAVSQDFNSPALKNKKHWNSFTTRFFLPLHVVESELASRGHLTIMAHAEQEQLEGQELRCHGCSKSMTFMAELKKHIATCDAVKRLPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYW0</accession>
    <name>A0ABQ5RYW0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47721">
        <property type="entry name" value="OS01G0235100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47721:SF2">
        <property type="entry name" value="OS01G0235100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="150" mass="15885" checksum="278A08B66E097178" modified="2025-10-08" version="1" fragment="single">LTKRDRIKMLSCTTMLPTRQCKPSTVFAGGVASKRAVMRLVRTQASDSAEAGPTTAADICSTCGVDRSKMPGGCDGQGRVIGGMGALPGFGWWPIKAYKPCPALYEAGLEYTRKGQMTNDVLFGGVSLGPQGAQSLEEIKKTADKGIRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ81</accession>
    <name>A0ABQ5RZ81_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">mRNA export factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005797</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="246"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="39119" checksum="091E7E1C65885CE2" modified="2025-10-08" version="1">MFGAPATQAKPAHNPNGDLEVPAPAEMDSISSLTFSPASDFFAVTSWNCNAYVWQYNSQGQTFAKAQNTGTQPLLTSCWKHDGSGIFLGGCDKAVRLWDLASNQAMQVAMHDAPVRQVAWCPQLNLLITGSWDKTFKYWDTRTPTPAHTGALPERVYAMDLREDLLVIGTADRNLHALFVTQPQQIKTLTSQLKWQTRCVAVFPDKKGFLNGSIEGRVAVSHLNEDQKDKNFTFKCHRADTEIYSVNTMSFHNVYGTFVTAGSDGTYNFWDKDSKQRLKAQAKAVYPNGTPAPITCGAFDRTGTIYGYALSYDWSKGYAEYNPGVMKPYIMLHSCKEDEVKPKPKAPTAGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZA7</accession>
    <name>A0ABQ5RZA7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005262</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF27">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="496"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="787"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="229"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1025" mass="105411" checksum="2658F04940FADE74" modified="2025-10-08" version="1" fragment="single">DALTSSRLAAGQVVLVGITSRVVQSLPNHGSPATAGWSPGPNPGLSNFGPALELIALGDPSGLPPQPESLWRLCSRRLGLPALADMDMDGDVSCSEAAAQVGTHLLPARVWPAAKKLPRSTLLLSPALRETAGQPPAGTVILLYCLSLITRAVSATAPIATAATTPAPAPRPAPGGGLGTAGIPPAVASAAAIPSEANVPVTSMPSGVGRCSAVVLQLCGEVLLQPRVNPNGSGNSSDDNGMRPSPTLGALAAKGIRNSGASVVPGHSPAARTPLPRTIGSGGVGSGAELAPAGGGGPLQQWPLDALRRSADAGDEVMRSALAAVARRHLAGRYLLEGAPLILPVLGSSVMLRVAATLAPAQADAASGRSGRGAMVEVGRDTSLLILYPGEPLPWQFMGQEDVSTTGVVATPGLGPGASVARRVSQAREAAMAAVPASAAEAAANAAERALLAGISAAGVTISHLGGVGQYLRNLRELVWLPLKAPQLFKQYGIRPPRGVLLYGPSGSGKTVLARAAAADAGATVFIINGPDVVSEYYGESESSLRGIFMAATALAPSVVIMDEVDALAPARSGGDGTSSHLPIVGGSDVASRLATALLTLLDGAEQDSMQLQPYRPVVVVAATNRPDALDPALRRPGRLEREIEVGVPTAEARMEILQARMRAVRHCLRPEDVVALAASAHGFVSADLAALVDEAAMCALRRRVAGGAPGRSHGEDDEPALEVTLEDFKTAETRVRPSGLREVAIEIPSVRWDDIGGMHSVKQALREAVEWPHKAQSALARLGAQVPRGVLLFGPPGCSKTLLAKAVASEARLNFLAVKGGELVSKYVGESEKAIAGLFARARATAPSIIFLDEIDGLVGSRGPAAATGGGGAGVSERLLSQLLTEMDGLLDRGGVTVLAATNRPDCLDPALLRPGRFDRLIHVPLPDKDARVEILRVQLRRCPMADDVDIEFLAVRTAGYSGADLAAVVREAGLAALEEDLGTERVAMRHFESALLLVQPSVSSSPNLMAMYNRMQRAVVSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZS0</accession>
    <name>A0ABQ5RZS0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">VA_PAR_042</name>
        <name evidence="7" type="ORF">VaNZ11_005737</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053276">
        <property type="entry name" value="MtDNA_mismatch_repair_MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48448">
        <property type="entry name" value="MUTL PROTEIN ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48448:SF1">
        <property type="entry name" value="MUTL PROTEIN ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="6">
        <location>
            <begin position="997"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="694"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="884"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="700"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="886"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI62906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1551" mass="163431" checksum="4C341AAB2CEFE223" modified="2025-10-08" version="1">MTGSSPKPLLGTVSALRESSSGGVSRSLQRWHLQWPSASCRARPSNISADQTARCRFPFKTGFVTPPAAALLQTCLFGGRPSLSQSHIGIPHATSVAFQNCCPAPYRHGFGFGAAFFCQGIHKTAAVPTGSLVRSTVQRHDTSTTSSHGLNFIGSMSYGSSGASGARRGARAAATHGTGGRGAGGPGGDKSDAVISSEAAAYWRKVLESIDRPGLGLLVASLDWNHPLGVTRPRARGRVQCYDTFLATKKKHPTAVVLVRIGEFYEAVGFDALVLCQHCGLNPMAPHTGVAEAGFPRAASSLRRHLNSLIEAGFAVAMVEEVREVRRAGSGFPSKERKLIAVVSSSSPYYLYGDVEDAYDGDDTPLPKPILGISVPSSGGYRLLRYCPQRRTVQVQSQLSTEALMSYLHATGVAQPLRVHSSVRDREREGGKGPPLARMLSAVLGMTVEEYGIGASTEYGGPSQLAFRRSSSAAPGAAADFDDVWGFRRVVAAQLGLEAREAEAMEVVPFVLESKYDIPLPPSLSTVTQLGLAGASGVPSLLSAVLPEGTPAPPREWMRRLLLLPPPPSVAEDIRVVCGQLADSAGDVLPLPPLVTSLPGPRIAALLAGGAAGHNTMRELRVMLVRVSELLSLPGPRAKIMHCALSGVLEWSLNSNAGLTWAQVPAEIFARHCAAAAAAIDEVVPPLEVTGAAGLCRTSDDEDEDADEDADGEELDSPGPPWASFLQRRALEAAALLVERMEKFRVAVREERLQAAADKVSACRTEMVSALMELVSALEAGGAAANADVRLAVHDDAVWARLVTFAGSKGCKMPATQQRALVAAVRAAAGLELIHPLNRKRGVEGDRYSSARLEAAAAAYRLAVDAADKEARRVLRELCRQLHGQDGAGSGPGGSGTAAGGAAGGAAGGGGGGGGGSHHLMTVLAAAELSVVFTALERHVARSKQAGWNWPLMPAVHSLSQPGGAGGREVQLALPGLWPYWMERREAVTNSLYLQAGRVALLTGPNMAGKSTILRSIAAAALLAICGLSLPAGPARGSPSFPATEVLRATHPPNFPATEVLRATHPPNFPATEPPADFSVTWLSHVSLRNFSGDSPLEGKSAFAVEMEDTAHTLEVAASAGRRCLVLLDELGKGTEVVAGSALAAAVVEALVRTGCAGVFATHLHDLAFLLQPLQPAGQLEYWAMEVTSARQQQQQPEPLPYRQVERPVLPVVLRPTRRVVVGQVCFRSLALQVAEACGVRREVLAAAARYEARLGDMMRHAREGMYAGQLGAVHLAVGSSSPSQLAEQEEEEEEALVAAEETLAADGGGGRACTDTPPGAWQAEAAVHRPALSGGSGAGDSCRTWTTLDAVDYNGVLGKKLTAAELLVIATGGTAADDAGRMQLLQSAAEELRALVRAAAEGAGRSERQDAGQLHWVSPEWRPAPGHAGEAVVYVLLRWDGLLYVGESEDITTRLKTHRAAETRERRARGKQLLAGGKRNATSLQCVYCVIPRESGGKSTARDVEAQLIGRLRKGPWELKSDFDKRRDAMHRPQLPSQTRALASVSGSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S090</accession>
    <name>A0ABQ5S090_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_006210</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF27">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN CTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0186">Copper</keyword>
    <keyword evidence="4 7" id="KW-0187">Copper transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045949921" description="Copper transport protein" evidence="9">
        <location>
            <begin position="27"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI63303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="206" mass="22112" checksum="5A249BB1926D2724" modified="2025-10-08" version="1">MFGQVANLLILCIVAVLLCSTPILRGTAPPPSVSMHSMHSHDHDHGATSSDLHQHTMPMAMVFDYGSKTTLWFSSLRTDTPASYIAVLLGLGLLAFVHEGLTAYRSARSSYPLHAIDQLDLLREPKLAIAAGSIGASSISTERLGQAGLHVASMGLSYMLMLAVMSMNFGVFVAVLLGFGVGFYVYHGERIFLSHPLVRGDACHGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S092</accession>
    <name>A0ABQ5S092_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alkyl transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_005922</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046378376" description="Alkyl transferase" evidence="5">
        <location>
            <begin position="31"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="407"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="360"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="456"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI63170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="56383" checksum="9B33AA1D66543574" modified="2025-10-08" version="1">MILGKFFNILLRRSIHLLLSLLGPVPIHVAFVMDGNRRFAERSHVDKATGHTQGYGKMVDVIHWCIELGVKYITVYAFSIDNFKRSPDEVETLMVLAEEKYIELAQDNGLAEREGIQIHIVGDLELPPPAVQGAAARLMQSTAALPRRLAALNVCFSYTASEESVAAVHEIQDAVRAGRLRPGDVTSEVLTGALRTGPTCPPVDLLIRTSGETRLSDFLLWQAAHAHLCYLPTLWPDLSYGDFAWCIMSYQRHACMLAALRRRAAMRLAAAVQPPRSLPKCMGGDSLSRKNQQRTQHCQPQLVGSRMSGMRPEGYGVQPAELAQGSEKLKAGWVSNAVFGACRGLLLYSSSSAATVNGTGNIASSSSGSSSRSSGIRSDGSSSGYSTHMVRSQLWSCLLRRYPDNPWWLLPLLPLLSLLSLLLLPLSWFVGVWRAMAAMYTSKGVELKNGAGVCGSGATTSSAATRGEEREEDAGDGTGRPDSEEAGSGGGVAGREVRLLRFFEGLEQARLSWIAGHVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0D5</accession>
    <name>A0ABQ5S0D5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">VaNZ11_006021</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 16">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="108"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="209"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="GLI63116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="306" mass="33089" checksum="DDBFD8D1E1942E47" modified="2025-10-08" version="1">MGDKNFLVDFLAGGVSAAVSKTAAAPIERVKLLIQNQDEMIKQGRLAAPYKGIGDCFVRTVREEGVGSLWRGNTANVIRYFPTQALNFAFKDKFKRMFGYSKDKEYWKWFAGNMASGGAAGAVSLAFVYSLDYARTRLANDAKSAKKGGGDRQFNGLLDVYRKTIASDGVAGLYRGFNISCVGIVVYRGLYFGMYDSLKPVVLVGPLANNFLASFLLGWGITIGAGLASYPIDTIRRRMMMTSGAAVKYKSSFHCFQEIVKNEGTKSLFKGAGANILRAVAGAGVLAGYDQLQVLLLGKKYGSGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0D7</accession>
    <name>A0ABQ5S0D7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005949</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125:SF10">
        <property type="entry name" value="OS12G0136300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="678"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="924"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1103"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1444"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1603"/>
            <end position="1724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1990"/>
            <end position="2029"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2059"/>
            <end position="2115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2131"/>
            <end position="2150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2177"/>
            <end position="2210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2260"/>
            <end position="2313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2349"/>
            <end position="2370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2757"/>
            <end position="2778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2801"/>
            <end position="2838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="463"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="508"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="927"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="953"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1121"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1633"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1686"/>
            <end position="1699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1704"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2090"/>
            <end position="2101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2131"/>
            <end position="2145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2192"/>
            <end position="2204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2275"/>
            <end position="2284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2838" mass="293527" checksum="4C05CDABD1A01FC7" modified="2025-10-08" version="1">MEEVQSPTADTNSPQPQPSTANWKLGENLALSHLSALPELPQYNAEDYKLQQLQYAMHLDSAMLTYTPAPGFLKNGDPQQAANRFDDSKTVKRKIFVNKEVLGRLVSVAEELNKAQAQIEIRKLLKREHRRRPSEPQPQQQPQPADGNPADGNSATIAELLAGLEVEGGADEDAAGAGVGYNGGRRLGARMAKRAPGVYRMAQVRCPAVLTSMAPKDRWGYFRAMQRLMPAANGGRPGHIAFGSMAPQLAPSGRARARVTGAAAVKAAKQLQMEVERQAEAALGEASITECGEEDGQGSAAAGIGGARSGVGCGGDALASLDATVQAALHANQVREARRAAARQLAEEEGLPTETWWDIMQKRKKVVYFKKLLDEMTGRMDMIKLHRAARVIQQAWRAHHRTRTRNASEEIALLQKELAHRKAMEGALRAAAEAAILDQQERAKKRAARLAARIAALNAMQEAAAQQAQQQTQHPSQSGASESHAGRGGNNGTFSRATSSKNLGWQHSLRSSIPSSMSRSASMSASRAASARRLELAAAAAGEGDDGGQPAYALAQNNLWSKIQALIGTDWDVDGEEDSEEEDDEDGYDDDDDDNDDEEEEDGDSEDEAAQRLRRRRERNEVRKARKAAADAAAAAAAGGDGEQKKKKKKGRRGRDESMAPMSKTTMLVKVVKQAMMAAKAMQDEDEKKGGGKRNRRKDRRSGGANADPATSMRMLATAAAALNMSVEAYVAMRNRKLGVTALKPWQPPPFPLLIEADGKPIAIEEHELREMEEEARRELLEKAAINFVTAATAAAGTSASTTFSGSAPGRTGSTMSGTASVALSADASSVMTWGSAAAAALAGGEGSERLLLSSLSLSENRSASEVAAAFAAAAAPSLTIPEDDVWTLVKEKLRQRMAARAADAHGGTVSAVSATASTRKASFFDSDSDYDDNEYSSSDDDGSSSAYDTGKTRQRRRDRPHHPNKHRSGGGAGGGALEGRRAKGGPLPGRRAGSYLSDSGYETMSEDEIRMAGRATLLRIQHQKERSRKHLQGYYTDRRRAKKAAAAAAVAALQAERAAALTTRHDALALRRRGAIAGPAWRPGGGGALQRRNHATATVAGASANDVAPTKSPPIRFWRPAPPRRPPGPLHGASVDGEGGAPRDSWSMEAAAPKPPLIRLGPYNLSPGGPSLLYREPRHRSREGLTSRERASVRAAKWMKGAEVPGAVFTQYGWVQARSNGAGNLLTLQLQSLPEPELVPVLEAYVRQLGIPQDGLGTAKTSLGCNRPHLLPGGHLPPSMPHQGRMQPLMVGGRSALDVRRAVSPSGGAARPPPAPNSVAAIFGAASAGFFVGHAPVIPHPPGHQPPSAVVRPPPPVAVTSPTRGPVPLRAEVGNTEDDVEAVEAMTSTAAAAGGSRAGPVITDKGDGSNGLGVPTVLGRALGSPTSSSRSLLARVRFNGEGQERTGLAGGSGSISRRSHTTSRRSMSPLLVVPGEEWPLRGDRVLGSAMTITPRIGAGSGSGTSFRSVSRGARSASTAFTAAASGGEFGEPESDGGVSAGAEDTFAVPRTESLQSPGQQEQDEDVAILQGEPFEVALAVLRSKSIKPGVAERVFPQGLAHINDPTSNVAPQHHEGDGGNADADGPMSYPLDDISSSSSSDNGDDGDGWATASDEGDRQGEQTTAPDVDADADAAGPGGHDEQPPRQSLQGGAVSQSIPLAVAPPATAGGPRGRAAPRTRQVHPMAAAAPARQAPDVCAVGNPTAITEAASSPSEVLLRNDENGERQFDLLYDMTRWAATRPSTPTNYRVEDHHIAITLRRSKEAKQRDQEEVHQVRRARLLAAYGGGAGAAVFGGNAYERVLFRRRHARALAALAASQSPLAQLLRAVAFDRQVAGKPLLQHHAAAGGGSAGRTGSFGDGLAVGEGRRRARRVGAAGGGRRLYVASSAVPGDLNGGVIWMPARGSRVDMRVKPGGGCVSGFGNSYYWRQPVRLTSLINIRPAAPAGQINSTDAFEGRGAQPRELTRARSRGVSARHTAAVPPPPPEVTEMANTALKLLSDLGSEIGRMEVEIQTLQRQAEQDELRRQGVSGGPGGDARPTQAASPVLAASHGTAATAPSASPPPPPRSPSGSLYRTQYITFHHQCHVQAQGPQQLAHQQSQQQRPASRCRVIPAPSNQRLTLMTLEPPTGIHGTSVPRMPHRQGPISPPQGRSTQGGRATQVITNGGTGGSSISTVTATAAAAAPPAAVGNRRSVTVGGGTAQPLSASVEVGRASTSTVALQGVAEPPDVTGSGEGGAGGSGSPPDDFLSHSAPLLRLSQPPTPSGACTVSPKLQNVSSVVSRSGSSFESNTAGQLGPVESALLDTVMPPGPPLTAPAASNAGGRRLASNPVLMPTPHNTLSSNGGWPSSCLSTISGMAPMAADAVPVSPQPPSPPVGASVPVWASPRRSITGAEPAAVASIARVVWELPSPRPAAAGTAAAAAVVAVPTPMAASQSSTAPLGRVGVHPYPRHNSPPTLLPNIGTIATATAAAALHPLRPSSDGVGTRLANQVATLSSSSASAVVAAAAAAGAAVAVTAKGSPSHRQGSAGHEVAAAPPSPPLAAWGATSSVLVGVLPEISQMSFSPRNYAPGQQTMRSSVSYSASGSPAGGAGNAAISSGGRVSTPLLVGSRAGPAAAASPPHSPLRNYPRMELGLGAGASPYRLRHGSSNGPSAVYYQYSVGGGGGGGTQVAINDGYDNGISVDSLLMNVALFGPGGLGGSGLGDRAAAGVRASAPAAPSTRWPRASDASLTMPTPLTPLAAPLTSTVSAATVAPTTDVTIGGSPRFTRVRRLSMGPLPGSGSSGSGTAAAVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0E7</accession>
    <name>A0ABQ5S0E7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006309</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="174" mass="19299" checksum="D862FF11741E4A24" modified="2025-10-08" version="1">MTDVKSEVKESTPALFAGRERKCYTCGEVGHIAHFCPKKNRSSAGASTSTSESSGGSRTAKLRYGVKCTWCGRPGHVEEKCWKKRDGVAKAVEKERTDVVALTLVSWTCEFIPHVTDQTKKVIAELESGVKCIRYLPWRMEKVAVLSAAVKKEISVLDPWWLHSGSKYHITGPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0M7</accession>
    <name>A0ABQ5S0M7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="847"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1070"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1178"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1263"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1536"/>
            <end position="1725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="606"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="852"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="894"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="944"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="955"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1005"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1070"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1184"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1198"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1274"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1313"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1554"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1596"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1629"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1695"/>
            <end position="1707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1716"/>
            <end position="1725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1725" mass="166861" checksum="FED16815412857E2" modified="2025-10-08" version="1">MGCICSKPVDKAEAGAGLPRGYDGERKGDEGKLHLSPPKQPSDSANEGDACSPREKEPARQAQENGVIKHPLPATPEVVASGKVIAVDRAPSVSGAAPTATEPSNTEQTIADPVVKGVPLSIDPRNETLAEVPAAECRRIPETSSAPSASDTKTPKTLMDDPSKGMSAAPGQSINAQESTAADVVTTAAVVAATPTSATGSPHTFPARGAAASTSTSSDCAAESNTAATADQEGDSNVLDSKPVANMPDGSIAISRYEGYRQTSADGEVTDKGVDQLVIAKKESAGLPVTAEDNTMAYTATATATAAIKDTRAVATAAASSPSASPPESSASANVEATASSVKATGSVVLRELPAVVDAAQMPLLAAPVAVERKELEASKTLAREMKEQISAITATTVSIPALAPVVQLTELPVPLTEAVSASVTATAVPSAAEAVAASVTATAMPSAAEAVAASVTATAVPSAAEAVAASVTATAVPSAAEAVAASVTATAVPSAAEAVAASVTATTVLSAAEAVAASVTATAVPSAAEAVAASVTATAVPSAAEAVSASVTATAVPSAAEAVSASVTATAVGVFFAAVTNTSQEQLPSVSHATASTSVHSLDGVQRRARQENTHPYDPDASGDHHVLHLSSGLDFGLRVVESVNRGDAAAAAEDDGNDDDDGAMAVTSISAARAPPAAATVSCPDIIAPPMARDEAGKSVVDPAVDASTAVPVRTSALHPDQDSEATGSTIDSIAAAVSGVPSALHLDVLDGDTSRAVAANGIGDGNERNGSSSPRPGVVAATAAALMEKQFAPPSFVLTQISSPSASSSPHDPTLQAAFAQVLTAKATTADGVRAARAEALRVSQASGVQGGAGSGGGGSVPPSPMSAMPPTVNVQNAVSGSASGGGADAGAGASASDGGYASDGAANSAVPTAAAASNASEEASTGSTGSIHGAEGTGGPDPDVAAAAADGDGDGDDDDDGDDDGDDDGDGDGPAADAFPSPPDDDVAAEEEVVRVVSETPEPPNDPPPTADPSAPADTTSTSGAANAPAAAAAAAPKPPPPPQSASWVRSGVSFAAIAAAAAAMSAEKTVTTTATGGGGKGSSRSMAPSPRNAKRQLPPALATDGAPGARARSAAVIAAGSDSGAGAGAGSTLGHAAITSTAAAAAAAAAAVEPVLANTAEIAPAAEAAAAALAAGAHAAREEATEPSKRPLSAGEARAAASSRNNSGGGTGSDSSSSVNGGGVVTGCTKQRSFAEIVALAAAAANNAGHSAAALEWKRRSGGGGGSGAAPSGGRAATAAGSKTASRSGSSSPKLRSGIAPAKVQVGSDGGSGGEGGGGGAAKEFPSDAQSGISINSLAADALKVASTMAAAAAASMPIVVAVRTRSRRKSGNVEEAADATVEAAAVASLPPTAVPTFPALKPLALPLPPLPPLPPDRRNKLSSRTLCSPHYKQPAAAPLAAAAPESINAAVATVTATTTDAVLPPPSWADEVEAAWEQEQEQVVASVPSAASAATVAVAATKEELLPWQPAPAAAAAPRVPALRSWASLLKRPEDGPGSDAGPGGGPAATTRSAAATPRALGSDHRQPFPGPQRAASVAGTPRVRPEFRSSAGHHSAAAAGNMVPPIVRASSASTRSGGGGANLSVGSSSSTSMAGTAPALNAELASPSRSAAAAAAAGGTDGDKDAAGWVEATRRRKARSPEPPRRGSGSGSGNGSGWKGGRVRGFQATGRSSGRSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S125</accession>
    <name>A0ABQ5S125_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="457" mass="51005" checksum="2AE92C9C34FBA33D" modified="2025-10-08" version="1">METHSPVVCKLLVCCPNEEKRRIDAEINLSVVDFEQLLTDAAGAKSISEVSVQVLDSEDEDGKLVAQQVAPVQLLSRCTRWLKKEISAPFKGKAWNQAQKKLTIHVSLKQPSVQLVSLEVSRISIRKDHDRGEPLKRSRSYEQAAINKAVRDNVPYNLDSSLKNPRVLIAKDIQREPHLQPVWDKCFAAGFAQVADLQPRVTVDCFKKKWQKYAMDYNAQRARYEKVKATHKEELACKPATPVPDGAGGTAQHTPGLGALELPKGTTKSGRNDEPPGTQDGRTSQRQKQPAHGEDALMMVLAANAARIHQKEKEIKAARRRQAKSSEPSKARNSSRNATNSSKAAAKKVTNNDDVRPSTKVRRTSTGIKRKVPVEDKSTDGDDNNDSYDHRRKKVKSASEDVRCTRARRQRGSSGSKKKQELLQEEVSKDEKSEDEQDDATSAREDDDNEDSEIEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1R0</accession>
    <name>A0ABQ5S1R0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PsbP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002683">
        <property type="entry name" value="PsbP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407:SF17">
        <property type="entry name" value="PSBP DOMAIN-CONTAINING PROTEIN 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01789">
        <property type="entry name" value="PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PsbP C-terminal" evidence="1">
        <location>
            <begin position="135"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="316" mass="34581" checksum="97035056E60A3B21" modified="2025-10-08" version="1" fragment="single">SRTLDERLTVLTIGVTGAVILGVTVHPCDTWTGLLRVTKMALRPISFTSSTGRVCAKKHITNKVNAITILASGHAQRHADVDDLHDSSQRTISHGALRSFNRRSLLLASGALVGSAPWLPALALEGSSTAQVSSVYTDPQDGFKLEIPPGWVFGEGELPGNSSFTGASGARRTLVWFPEGANPRDVNVTLVITNVSVEFTRLGSFGSPYQFGSSLVNSQDRSYLLRAPQWARGKDPIQIAKLVDASEMSNRYFVEYTVQKLPEPQRHLYSLLALGYNGMYNRLYTITAQTLEEDRSQFEATLLAMVKSLSVPPQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1T2</accession>
    <name>A0ABQ5S1T2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="437"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="482"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="534"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="640"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="653"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="666" mass="65973" checksum="ED93BFC05C4DEAD3" modified="2025-10-08" version="1" fragment="single">MEADVYTVPSEVLFKNYSTSTGPQTRNLLIKNTTSQALHTVISYPFSPSFRLSAPAEFRAHLKPKPPHLILELPPKSTSKVIVELKPPSSTAADGPQFLHDSLLVLAGSVCILRVPVRAVPGAEPVPAAAVTSFWPPQKEIDAHCDSFTSQVKAMYDTLSASRPAAVAAVPAVLRPAAAASVTELMQPGTALRAPMVVGSEVAESSGAASGALRSSSMGASGTSAPAAAPTLLRSGSGSTAAVVPPAAMVAAVIPAAAASQPTPELPPEGFSQPEDLYDDAGDVLYDEDGDVVQQPFVLHKTVKVQGRVAVAVPVDGGDGGAAAAAVGSTSNGRSSGGPGLLSLPPGNSLSRTNSTSSFTAAAGGAAAVGLGGNGSSRPGSASGGKLAFALNGKLYDARGTLLDPSLEQVSAVGSLMVVDCGEGAPSLSPSPPQQQPQPQQQQAIRTSSSGTSLTSLTPPRLSTPPGSGNGSGGAGAGGSATGLSPGPGSSRVSLAPLKYGNLPVTASLRPGSAASSMSPHLGRPPPPPPAYTGSAGPGVAAAAAAAPSSAAGAGAGGSDDDPLRKAALAAALRELGIRDLPTPSPRRPPSSGQMPTITVDGLHQTAGAGTLLSGLSPPSYLQYGLPPNYRPSSGRRRPPSATGSAAAYGSGGDEDADGHDDADLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S203</accession>
    <name>A0ABQ5S203_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006866</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="260"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="325"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="506"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="587"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="646"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="262"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="325"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="390"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="676"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="757" mass="79245" checksum="5D918D088E9F12B6" modified="2025-10-08" version="1" fragment="single">NLLRRLRHDNIVRYLGTERTDDCLNIFLEYVPGGPISNKLAQFGPLREETIRVYTKQILRGLEYLHQQKVMHRDIKGANILVDTNGVVKLADFGASRQIEELATIGGGSRSIRGTANWMAPEVIKQSGHGRAADIWSLGCVVIEMATGRAPWSNFSDPYAVMYHVASTKELPAMPDSLSPHAKDFLTLCFNRVPRERPNATRLLQHPWLQTVIVPRGSGPPPSLLPVPAALPQARPAQPPSALVSGQPSVKVMVPPQGCHPLLPPPGLRSPPSPINEGKSESLFGSPAGGITTAGSSTPTSTRAPALSTAAVAAGAAGGMVLPPLMSPPQTVPRPAVPPLPLDAMKLGLLTPPQQPPNPPTQAAGSGALTSPGMLPPPLPQPPAAALNATPPPPKPPLPPLPLPSASRGAGYQNQYDTLVDSEAVHLQLQSIRQQQLQQQLQQQRQACHMQDMYDSICMGVGMTISIGADSAGAIAPTMRDINPILEPSWMPEQILQHRAFFDQMKKMGTPPETPPESPSASTVGAGATSKAVTGSGAGSTQGSSSAGADAPCSNCCVAEHTKLPPAVAVANDVTVVVSSNPHLAGGAALQISPRSGAGTHRSGLGTWRSGAGTHRGTARNNAPSRPVPSRPPLAGLFAAAYEQENGGDTHMEPDQPLSARQGSSAVGLSPRVLHPRQPSAQQTPSQPPRVVSASRRALEEAGILGAATLDSARAKQWRDELVAELEAERLRVGVGTSMATVAVVPATGARPSVADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S209</accession>
    <name>A0ABQ5S209_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_007003</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03253">
        <property type="entry name" value="ABCC_ATM1_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="496"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="528"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="364"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="708"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="33"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="271"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="965"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI63886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="988" mass="104289" checksum="44E9E92F24FB823B" modified="2025-10-08" version="1">MLTASRVAAVLGAGLRQSNLPAMLAEGVTQAAGSLSPAPATPPVPCLSGREPGSVSEGEMPLPNCTSPWSSSSIVKSSRDWDGPSNNSSRNSSSSVGSRIGMSSSGCCCSLCAGNSSSSSSVEVQQWPSLPAADGDTNSCVALLEPSAFRGRRASGCAIQTVYNCHHNQAAHIYALQLAEMQELGPVRRPLGAQTRLAPWNLQPRPGGAVRSLASRRISTFAGFYLHSARQQYLGGHPVHIIRRDLAQKAAPVTGMGPAVPSASRACAHVSLPATSPPSRQEATHPHVAAGGSAPLQPSHPTDTSAFAPDTSAFATVAKAPGSILPQFTPLEEAAERLSDAQILRRLMGYLWPQENPEFKRRVVAATFLLVAAKLLNINVPILLKLAVDALTSAVAASAAVPAAGVGTAAATVPTVMAYGMALGPITLLLGWGAARSGMALFNEMRNIVFAKVSQGTIRRVAREVFSHLHRLDLAFHLSKQTGSLARVVDRGTRGINFILSSMVFNVVPTVFEVTVVTAILTSKCGPALGFITMGTLAVYAFFTLAITQWRTQFRRTMNRAESEAAGRAVDSLINYETVKYFNTEAHEAARYDESMASYESAAVKTQQSLSYLNLGQSAIFTAGMTAAMVLTGRQVLAGQASVGDLVMVNGLLFQLSMPLNFLGTVYRETRQSLQDMGAMFGLLQQHPTIKDSPDAQPLPPAAKGYDVTLEDVSFGYRPDDPILSGLSLHVPAGTSCAIVGASGSGKSTILRLLFRFYDAQSGSVRVGGRDVRQVTLDSLRAAIATVPQDMVLFNDTIYYNIAYGNLGATRAEVEAAAHMARVHNAIEDMPDGYLTVVGERGLKLSGGEKQRVAIARAFLKAPQVLLFDEATSALDSKTETEILEALRLLAQGRTSIFVAHRLSTAAQCDQIVVLDDGKVVESGSHSELLARGARYADLWSRQANVDDLGAGSHKVVSWSWARSSNDVGSDSKSNGAGGASSGAAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2G9</accession>
    <name>A0ABQ5S2G9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007264</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_eL15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="166"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="204" mass="24208" checksum="D195ED54AE4E5496" modified="2025-10-08" version="1">MGAYKYVEELWRKKQSDVLRFLLRVRCWEYRQMPSIVRLTRPSRPDKARRLGYKAKQGYVVYRVRVRRGNRKRPVHKGIVYGKPVNQGITQLKPARNLRSVAEERVGRKCGGLRVLNSYWVNQDSCYKYFEVILVDVAHNAIRNDPRINWLCNPVHKHRELRGLTSAGKHYRGLRAKGHKASKTRPSKRASWKRRNSLSLRRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S302</accession>
    <name>A0ABQ5S302_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TRP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007496</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="16100" checksum="6BCC5B77132FDCB5" modified="2025-10-08" version="1">MLQALKDFQIQRFNDLVALADIAQTAWQQQQLPASGDVPKSTQYTAMVILVAAQLVMILLSRRGRRIIFLTVDTVLAVLLVVVLLIAILSLPIGAVYIGLRGSIMMARSLAENFTALGRVLRPLLALIGQAAATPTGSGGSGSGVADSAASRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S315</accession>
    <name>A0ABQ5S315_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="46974" checksum="AB75891776843567" modified="2025-10-08" version="1">MASSSDEPRGPLDTFAEDLGRAVLDAAASGNLSAPALLAAATETGVRPDQLATLIAQTTEIRTQLGKYLLSLLNLLKSTVVNTNMDNTAGPRVLPLPQARGHDDIPEVMRLSIPVPRLNISLTDKTPGHSIADATRAYVRDAKKYLQAVHGGDNDGIRCLFVGNTLEGAAKDWYRNWTNARGSYTFDELVAALLARFATEVQARDVEARNALAAGSYHMRRDENVPAYQSRFEALVTPIADLTESERVFWFRRGLTKLLAKWCATDLVGAQIKSYDALVAFAKGAEIRLLAGEEEVKSAPRANAVQVQDPLGEAVALDQDLGATRPTPTAATRRSNGQRKARGGASSGVRGTGGAGPSSDPARNRKRGGAPSSAAAQRSDPNAKRTRTDDTFMPKWNCNRSEFERRKLANICLRCAESHSTKDCPLMPPFVPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3G6</accession>
    <name>A0ABQ5S3G6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_007290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF18">
        <property type="entry name" value="HEAT SHOCK PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="100"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="338"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="414"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="458"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="532"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="989"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI64115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1250" mass="134964" checksum="E9F858DE9C17171B" modified="2025-10-08" version="1">MTMTQLLWLYRQDSAGQLRFSSQLYQWVSRRFSTAAEGGSEPADYGRLEQPSQNRRRVPGAALQRFGRDLTEQARRGLLDPVLGREHVITRILQILLRRTKNNPVLVGEPGVGKTALVEGIAQLIASPHALPGLAGKRLISLDVTAVLAGSSYRGEFEERLQAVVADVGAARGNVILFVDEMHMLVGAGSAEGGLNAANILKPALARGSLRCIGATTLDEYRKHVEQDPAFARRFQSVLVEEPEEGEVVAWLRGLAPRYEQHHGVAFTEGALRAAARCAKRYVTERRLPDSAIDLMDEAAAHVVLSRERIREVAPEPLGGLDVPGQREEVSSGLRAVNTALGGTGPGSHPDSDSGLLPQSAYRPSHHHLAQPQPPRQGCPTELRNHDDRKRELVQQWQQERFLQGRDHGQAVEQSLRGGSRAGGNEETCRQVPFPVDEEPGAHQTDLNMEKHSSPHIPHLALRQGDDGGSGRGGAYGCGDSGPGGGRQAELRSGVEVPTSGFRTLEDGLRRSLREDAARVLQAMKPPLEAHNQQLDSQQHKRKQQLEPGQGQGAVVRDWSSWVSSQGRQSMEEARQQQLLEWFGATPLQPKSGVEGHLEMTRERQRRHAEIFGDFGTSTEAATSSDDATLAAERAQPKACPHCGTTVTAQPEDMLLRCPSCRTRFLNIAPEKLQLGTSVLLMLQQQQLRHKAHHYDQQQHHHPAHDYNQPHEEYRQAQKQLPQAEILPQLKHVEYGRITDGGASGVHCSQETNCGPRCRALGPGTSGSTSSKSRGIVRDSGNGLRVTEAAVLDVVSRTTGLTLDAVLRDHFGASVRHLRTAMEARLVGQRDAVRAVCNAIQLHRLGLAAAATTAATGLETSGREALSSSVPFPRSVSRPVASFLLALAEELFHDPAALLVLHGGEYSSRTSIARLVGAAPGYVGYGSGGMLTEALRRRPHCVLMVQGVDKAHYEVQELLLRGLQEGNILDGQGRRASLRNTIVMLTTATTTTSSSLRPGHSAGPSQAPATKRHSGDAASFPSMAADSAAEWKGSGGNILDGSGSALLPEVWTNVDRIVHFHPLDERQRTEIVARQVRELAAQLRPAGLAGIDLDPLAAAWLARAAVLTSPFTQGLSRLPTDNNQGIGRGGGMAGCAEVLRRQILEPLAEMLLENAEAYTDNGMETGQDSSERAVEVAAADVGQLNAKYGSQQRHWIARVQLVQGGGTEGGVGVGAAQVEEGPLALELVEARGGPRGQSVRANSGREWVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3S5</accession>
    <name>A0ABQ5S3S5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rad4 beta-hairpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_007283</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.20.110">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2460">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018327">
        <property type="entry name" value="BHD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018325">
        <property type="entry name" value="Rad4/PNGase_transGLS-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018326">
        <property type="entry name" value="Rad4_beta-hairpin_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018328">
        <property type="entry name" value="Rad4_beta-hairpin_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042488">
        <property type="entry name" value="Rad4_BHD3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-C CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135">
        <property type="entry name" value="DNA REPAIR PROTEIN XP-C / RAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10403">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10404">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10405">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03835">
        <property type="entry name" value="Rad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01030">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01032">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="7">
        <location>
            <begin position="714"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="8">
        <location>
            <begin position="885"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="280"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="394"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="565"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="800"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="433"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="574"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="822"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1096" mass="113329" checksum="663E343B213E0283" modified="2025-10-08" version="1">MMAYGGTEDLEWEEVEEEDWEVICVDDEQKQGAHRSGIEFVLDDEGPERRAKHVITKREREAAAAVHRSHVLCLLARVLLLDWAAEQPVVQATALSLLSPAAALVNQSAEEAQRAINGMLPLATWFRNTFAVLPPDLVWGREHRREGRRGSSLRVEDELDEQLLRTARSQAGTVECLVALFVALVRAQGVSARVVRLLDAVPLAPWRAKPLTVRTAAAPSGGSRPRLALDLEAGDQLPPQAATVTGQKSGSGSMGAASRAKKGRQLEEVLAEKGYGVRKGKKQKVGAGSESGSWAGGPGDGVISTSKTAARSKPAPAAANGATEITQKNGGKRGGGDVGTVAGGNGSAVIAAAERDNAGVELVATGCAAASRPNKGEEEFERQLQMALLATSMDPAAGRQKQQAEAGADKAGESGTGSLRSQSRRGGVGKAAGSGSVAGASSSGSGAPVGTAMSVRPEVVVNCWAEVYCGSVARGRWVAVDVANGYVDRPELVDSATLRPTPVCYVVASERGALLDVTPRYCHNLLAAQRNRDEAWWATTAERLQRVAGRCLGRAMPFPDAATVAVGPCNDSDPGDRRGKEEEEARPGVKVVAMPKEEEGSMNVMVEKQDATGKSGTSCGAGPRIIDARLSHQRGEMENPLGPNAAAIARAASTSASAFAEVAGTSASGSAARSVAIGQAVRSGRQDSGGVSASGADLRAAREAAELQHRGLSQLQGLPTSIEGFKNHPLYVLKRHIGKYEALRPRTVPLGLHRGEPYYPRSDLSVLHTVERWRREGRQVRDSELGAPAKVVKKRLMGGPGAAATGLDHTPSMNAGGKLKGRAGGGSVGGDDGGGEADDDFLMLETAGEAVTAGASGTEGGPTINLYGRWQTDEWIPPVAERGIVPKNERGNVECPPLVPALPSGTVHITLGPGLGGVCRTLGIDYAPGLVGFEVQGGRMVPKVEGVVVCEEVSELVVAAYLERESARAAAAAARLQRAALAAWLRLLGALRMRLQLERDYADVSAGGNQGVSAAARAAAPGGSTATAAVAAAAAAAGRDAGIKPRGAVAAAAAAAGSTAGGQCQVRFPSAAAVAASEPQTTQTIGATVDVEVEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S698</accession>
    <name>A0ABQ5S698_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="265" mass="28077" checksum="699D7F67DB0DF040" modified="2025-10-08" version="1" fragment="single">LAPPLAPPLAPLDSLPQPLGWPPSRSLPPSRSRQPTVPTPNPPDPPPHPSLPLHHPPSLPSRGPANPPALNPPPLRPPPLRPPATELPSSPLPYPRALMPPSPLTPSPAAPSQPLSPPPPSTPEPVAPLEPPTPPPPRPVALSSQESPSPTPQPGPPPAVPEHPSSPLILSSSPYRKWSPPLPSRPLFKPPPSPRPPRPPTLPRRPRLPPPPSPPPCLTPPSPRPPRRPRPLPWSAHQLPTRSPPPPQPPSVPDVPLPFSPEEMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6T4</accession>
    <name>A0ABQ5S6T4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11840" checksum="4E2ED5A77DC62E35" modified="2025-10-08" version="1">MGKDRLDEPLDLHNYSTWKLRFQLLCAELGYSRALLQAPIAEADVIISDKVKSVMAKNVKNHYLEAIVRAANAKAAWDALAATFASTCNTRKVALRQELSDFKMAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7C2</accession>
    <name>A0ABQ5S7C2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YdbS-like PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008815</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="YdbS-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35688">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35688:SF2">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YdbS-like PH" evidence="2">
        <location>
            <begin position="125"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="203" mass="22080" checksum="B2D4C89947AEA83E" modified="2025-10-08" version="1">MLSSLQATSASTSRPSTGFRKHSAYLCTKLCRTRCSVSQTTLSGNRSQRTVATKASTEDGTAASAPAAADVPAASTSSDAAAEVTPEVVFYEGPGSNAELVLSLVLLPTLIYAPLSIASIGRRLWISFKFTNKRLVIVNTSPLFKRTIEIAYKNIKEIRTGPRGFGLWGDMVVFLRDGSRIELTGLEKFKEIEEHINRCIYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7K8</accession>
    <name>A0ABQ5S7K8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glyoxal or galactose oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_009535</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02851">
        <property type="entry name" value="E_set_GO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.80">
        <property type="entry name" value="Galactose oxidase/kelch, beta-propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037293">
        <property type="entry name" value="Gal_Oxidase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009880">
        <property type="entry name" value="Glyoxal_oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015202">
        <property type="entry name" value="GO-like_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208:SF21">
        <property type="entry name" value="LOW QUALITY PROTEIN: ALDEHYDE OXIDASE GLOX-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208">
        <property type="entry name" value="SECRETED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07250">
        <property type="entry name" value="Glyoxal_oxid_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09118">
        <property type="entry name" value="GO-like_E_set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glyoxal oxidase N-terminal" evidence="3">
        <location>
            <begin position="242"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose oxidase-like Early set" evidence="4">
        <location>
            <begin position="590"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI65892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="706" mass="76776" checksum="E383BAD8D9CE23F5" modified="2025-10-08" version="1" fragment="single">PPPPSPLPPSPPPPFPPPPFPPPPFPPPPLPPPPSPPPPFPPPSQPPSFPQPSGPSLPPPPSLPPFLPPPPRSPPPRPPPSSPPRRPSPSSPPFTPPRPNRISPPPPPFVQRPPPPLLPTPPPPLPQGNTAGGGGGSSDAYPPAPPMAPNFPPVTPSPPLVARPPAPPAGDARLIGRYDEIGIGMIVAVHLAQIPGTDRYLFMERPSGYHPDNSKSIAGFFDIPSRKFTHVFSPDGLFCCGHTILDSGDVLIVGGHQANAGYPDGMKSIRTFNRSCTDLQLVKIREMGWRRWYPTPTLLPDGRVLIMGGTQGVGAGTSNNPFWEMYDPTTTRITPYAMRPMYLDQSEQIYYPFNYVLPEGLLFTFCGRAGYILDWRTNQWLQDVPRLRGYGNTQFPFTGSSVLLGLYPESNYQVEIMLFGGQREAAVDDLSITANRASGRLKLKYDRTSGNYTFDGWISEQMAIGRVMPDSVLLPNGKVIILNGAWKGLAGDSASGGDSRANYPILFAEEYDPKAPVGARYRRMATTRIARMYHSTAGLTTNGTILVAGCDRCYRYLVQPGYDFDPSPTSKAEYRVEIFSPPYFFMDEFKPSIVRTSSNIMPYTEAFTITYTFPAGWAGNRLTRVVLVAPSSTTHSYNTNQRLVGLEIIANVVGPVNGVVTVRGPPTINIAPPGMFMLFLLNGDVYSRAVWVTLPRPAPGAPGYMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S811</accession>
    <name>A0ABQ5S811_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="177" mass="19761" checksum="2023660BDDFB155E" modified="2025-10-08" version="1">MVAKCRAVRALLPSVLNQVEQVIGRRFSFDARTVCAGFDGFCNLFEPQGRPLSSANLGGHTILFTPSSLCELQKQLAHFRACKRQHPDTSACILLPEYVSRRNPSFLSGMQRVLRFNPGTALFHSLDMAGEVVQCCTSRSRLLISICTSGDFRHRCASTCKYSKASESKQRQRKTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S817</accession>
    <name>A0ABQ5S817_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009247</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF373">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWIRM" evidence="3">
        <location>
            <begin position="375"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="635"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="881"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="301"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="638"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1124" mass="118148" checksum="E96B76860F97A872" modified="2025-10-08" version="1" fragment="single">MDVDNPQALDRKVKVLHEARPPAPTLLQPPDTTSPPCDSDNGAPALGTGNAAAVATGLGCAAEGKGSALLVPAIADVGDAVLPLSTESFPAGSLLADAIADATAGSAASMQDPMETEHSQLNQQPTHESQQGKVQQQDPSKPSLGQDALSGKLVSMGILSEPRRDPPSGGYKGKDVLTAGCLGDPGQDEAPAPRAPLSEVLEGRREKDEQEQWQKDEALRRETEQRPGMELQESEAELQQLQTGTEGAHYSEPAEQQLQSAEAEEQPMETEQQQEEEEEEPKQDSQQPRRPTRRRVAPTKLSDSVSARLPQQHSDGIGATAGGGGSGGHKGGGLPRTASALQQQDTAGAGGGTSAGEGRPTKRSRVHQVSEERLQEEVVQAVGLDPWGLSEEEEALLSDVESQRPGQPPLGRHQYLKIRNLILTMWRLDPRRHLTMEAACKAVPTQCTRHAEVAWSYLHSYGFINFGLAVASVPDMEHEETIIVIGAGLAGLAAAQQLRQLGYRVLVLEARIRPGGRVHTARMEAGGVTGFAELGGSIITGCDGNPLAVLANQIGVSLQEVRSETPLYWEDGRPVDEALDQQVTQLYNEVLTRCDTLCHQLGPRAGELISVEAALSAIWPEVSAAAHLRDRNGGSAGGAAAAAAQSSEAGSAGAGTDNEDSDKLPDHLFNWHVANLEFANAAQASQLSLRHWAQDDAYELQGAHTFTAGGNSRLVQLLTQNLPIMYGCPVRDVRYSSGGSNGGVTVVTEAGTVFEAAAAVVTLPLGVLKTETVRFSPPLPAAKQEAIKRLGYGRLNKVSLLFPYVFWDPSVDTFACAMRAKERRGEYYLFYCGAYTGGAAVLTALVAGSAAIAIESISDEQAVAQVMRVLRNIFDRPHDEAEGSAKAAGTAEASDGAKSAAQQEKLGGGGGSEAAAAAAAGTTGTRKLAVPEPLQAIVTRWGSDPYSLGSYSSMAVSCRGAAEYQAMAAPVGGRLFFAGEATMYKYPATMHGAFLSGLREAGRIHYTFARARHGLPPRADGFTGAAEGGGDVLAAATGDCPPVAAPPSGPALRHVRRLAALGGNLRALFSAVEPDLEFGCFKGLFGPEVPGKTQWALLQIDLGCLRSRTASSSEAEADDADVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S864</accession>
    <name>A0ABQ5S864_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="645"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="781"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="874"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1095"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1417"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="428"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="554"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="666"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="795"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="814"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="836"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="901"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="911"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="951"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1419"/>
            <end position="1438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1478" mass="153778" checksum="0A0E5372A17F58FE" modified="2025-10-08" version="1">MGSGDAGGVLVSPAPASMAPLTTAVGRLLEPELIALSSHGGHHLFINRIPPRLPKEDVEKIILAQLRLHPFVLQVSKCSVIPQNSLKNKGYGFITLDGSPSPTDLDAIADSLNNKLQVGCRTLGVRVCTDCHSACYGEDDGCASSSSRCSTPPNEPASFDLPDAQPRGAIELSSGNDIEAAAAVKGDVLCLAEQGSAHASMMGAQVPQADILQNAVTVLVKQNFAGPASVGGQGLRRRKAARQDEPRLYSENGRYHLPQHHHLVQSQAYQSYGGQEDSRWRGGKGLYNGNNSAYAAGRQRLKIAESGPSTTAFGEVAPRISRAPGVQAREGDVLERETGIPDSCDLPQLLSQPVAPLQSTMPLQQQHSVSIMKQEQEQLVLSAAAPQNAGDCGVNGAMVSPRSISSYSSASGSVLGSTSNSAPGSVTGSGSRSASGQMSRSSVGGWCGGDADERAVASSGPNPQELVGVSSAGTDADIAAAADRVSDSYSFNQQSLSSRQHQPLQRQVQGTPQRPAIVNEVQGHSDVADDALASAPPHSPSKHDHPASGAMSLQQEQQQGIQQAPPSQAQVQRRHVHTQPQQVSAQSFGFVARRSRSPTHEARSVPTVAMAEQMSAGSQGHLGRGQVIAVQPSPAAQMQSTALSSTQQTSGFRTGIPAHSHVHSASAPTPSSRNLQQVPPQGRHRSTFHRQQPKLMQGGPYAYMMASSAGSGGDGIGGGSGTQRSGFGGGDFQAYGSAYTRLQRPCPVQIVDEAEMQVSGYGCQREGPSSAWEGTESAVVEGPGINMDRRGSYPGGQSSTRQYFATRSQPPPAQYRQPQQPQHQLVPPQQSPHPMSFTQQSRVVSYQSNCASGPGSQPLQQLLLAQAARLPAQQQASGLHFHPLPPPLSQQQQPTGICFSQHPQQQFQPQEEVTSFGQSPYQRHQQPQLGREGQQPVKHPQPLPLHPSQAQQRPPQGSLLQQLSQPQQSLCVRGFEAHAMGVPQPQLLPSTQMLSSSCVPPPMTSTGAGLMKLQLQLMGNSEVGSDGYRLTDSAPNETSIPAGFVHWQMGNLDTEYDLRECSSRMGKPQLTITATKETLTAAAATSMLKVQSADLPLGRSSSDGVQDPGAESTDSGDLDPLDLEPMILQLLENEDDDGCSGVGSRQLSAESSQQQYPHHHLHYLQSNAVQQQPKLQQQAVGTSARLGVVPASATLQLSPLVAMPAAGADSYKYGHHQGHQPRHHHQQQQLGFTTRMAVGACGGTSGGVTVAWAHTTTKSYTDGGGNGSGVDATASAGTATAGALATSSLNRVVALSYGGAGMMPSGPVAGGALHATTVPLHEQLTAAPSPTVTAEPSWGMGRGDMGPSHPGVGMVGSFPGPDAYGGVAAGVAHRLGPGAGGSRAPPLVANGSFGPGGRGFGQRGSRHHAGRIPMAHASYGGGRGSGPRGGAMGTAGMGAVTGGGRNKGGGAGGFEAQGLVSGSLPNASLLAVMQRLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8C6</accession>
    <name>A0ABQ5S8C6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Molecular chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008832</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45295">
        <property type="entry name" value="CHAPERONE PROTEIN DNAJ C76, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45295:SF1">
        <property type="entry name" value="CHAPERONE PROTEIN DNAJ C76, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13370">
        <property type="entry name" value="Fer4_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="57"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="157"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="42236" checksum="56B76D9CDDA613DB" modified="2025-10-08" version="1">MALSTGLLRNSIPTSCSRHCQPFLSRAPGFGARAQRVTCAGIKTETNNYVLSSGIVDYYEVLGVDDDAPTEEIKKAYRSLAKECHPDYLGDRGHNICVLLNEAYQVLSDPEQRQKYNSKLEQALLDEDDKFTGQPLSRWVPATKPSMAKNTDPAEHRAVFVDEFTCIGCKQCVWCAPATFRIEPEHGRSRVFAQWLDTEDNLQAAIDGCPVSCIHWVDRAELPALEFVMQQKMTTRVNVGVMMAGQGVQMDVFAATVSFMKERRRKEEARARANKYYSPQQEEARRRAAQELAKQHLGFFAQFTSAFETAFSGMNQVVSTGQETEDLKQVGRRKRSQRARWDWLEQQRARGGWMVPPERALVPISVYAESLHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8P8</accession>
    <name>A0ABQ5S8P8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="106" mass="10789" checksum="CAD3CD95FD3748C1" modified="2025-10-08" version="1" fragment="single">QSRARTAAQGPGSAAAAAAAAAAAGDLEEVPLAQRRTVARARLYADQLRVEMMRRNWESLRDVETVEFGQSTLGAEYRRSQQEQGAGAGAAAGGAGGSTSAAAAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9D1</accession>
    <name>A0ABQ5S9D1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rhamnosyl O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_009417</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007072">
        <property type="entry name" value="RNMT_CmcI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40048">
        <property type="entry name" value="RHAMNOSYL O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40048:SF1">
        <property type="entry name" value="RHAMNOSYL O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04989">
        <property type="entry name" value="RMNT_CmcI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046304500" description="Rhamnosyl O-methyltransferase" evidence="3">
        <location>
            <begin position="22"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI65796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="38378" checksum="71A9C64594E775E5" modified="2025-10-08" version="1">MTWRKLFLLTVQLLSLKTCNAESAIRDPSIRIASLLMRVTRKLDTIYKLQEEVMAELDEIHSLRKISEDNGEIKQTIHEVECTGGVYSFDRASSFRSANETVAMNNGHVVRLDDILYGYDYLFEQKHLFSAGSWLGVQSQQDPWDLVAIQQLLWDVKPKVIYDIGTNVGGSAILFAHIMSQYAKPREALIVSIDPKDFAVNWDAEAQRLCPRCTAVADNPLWGQYVRFVKARAVERSALAALQEAISTFGGPVLISVDGWHRYAEVLKQCQVLSQYVTIGSYIIVQDTKLDRFLRKAGPRRAVYDFLKNNTDFEIDKSREAFLYSQHTDGYLRRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9D7</accession>
    <name>A0ABQ5S9D7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_009976</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="106" mass="10471" checksum="BADCABC16F07A701" modified="2025-10-08" version="1" fragment="single">GRISGGSPTAARTAAAAAAAGRTFATSLHAVAQLGIRLQPAETEAAEAAALTLAPYMNHLADCVMVLSALPEVVVPAVAEAEGRPLEAAKTGVMGNVRVVAAVPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAU3</accession>
    <name>A0ABQ5SAU3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010503</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045356912" evidence="2">
        <location>
            <begin position="25"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="602"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="725"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="755"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="857"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="875" mass="91721" checksum="F0E7574BAA28A906" modified="2025-10-08" version="1">MVSPFHCMLLLSSILLCPVLLTLAKVPTVSLHQSQVTEVKDLYWKNGDNTLEAQLQTLGLSVQLAGEPTNTGTTGAPDAYIIPVQHDQVIYSSAEYMDAVSFFVASGGLVILLDAGTGDGAFIRNFISEALEYKGKWHMCKYTCIAGQLRRSVHPALSRSAPFFLPDVGHTSNPWPRELEGAQATRLSTWCHHEDSDAVTVPLYTANGDEDTLLVAQAFGKVGVPGAVVWLGYSWKAGPQEQWGALINKIITDFAERVRSPTFIRGLPDPASLQKPLAMPEATIDIDDFQDTIFHRVLTSMGAYPSPEPPSPPPPSPPPPSPPPPSPPPPSPPPPSPPPPSPEPPSPSRPPRPPPKKSLYPPPKKDKEDAKPSPPPAKASRPPPKGKAKGDDRRRALLGPGTYPPPLLPSDPPTSPSPAPPPPSPEPPSPPPPSPPPPSPPPPSPPPPSPPPPSPEPPSPSRPPRPPPKKSLYPPPKKDKEDAKPSPPPAKASRPPPKGKAKGDDRRRALLGPGTYPPPLLPSDPPTSPSPAPPPPSPEPPSPPPPSPPPPSPPPPSPPPSPPPPSPPPPSPEPPSPSRPPRPPPKKSLYPPPKKDKEDAKPSPPPAKASRPPPKGKAKGDDRRRALLGPGTYPPPLLPFDPPTSPSPAPPPPSPEPPSPPPPSPPPPSPEPPSPPPPSPPPPSPPPPSPPPPSPEPPSPSRPPRPPPKKSLYPPPKKDKEDAKPSPPPAKASRPPPKGKAKGDDRRRALLGPGTYPPPLLPFDPPTSPSPAPPPPSPEPPSPPPPSPPPPSPEPPSPPPPSPPPPSPPPPSPPPSPPPPSPPPPSPEPPSPSRPPRPPPKKSLYPPPKKDKEDAKPSPPPAKASRPPPKSKDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBI5</accession>
    <name>A0ABQ5SBI5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010811</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000042">
        <property type="protein sequence ID" value="GLI66833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053128">
        <property type="entry name" value="Cystatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12319:SF2">
        <property type="entry name" value="CYSTATIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12319">
        <property type="entry name" value="CYSTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045119601" description="Cystatin domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="3">
        <location>
            <begin position="27"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="138" mass="15070" checksum="A275F7E8AD6C7BD7" modified="2025-10-08" version="1">MKKYFIVLCSFLVLSGIYMEAKSDHGGLAGGIVEVDAENPGIKAAADYVVSAANTNSCNGLCSSLNREGELKLVKILSAKTQVVAGVMYNLELLVEDKNGQQVIFNTSVWSRPWLAGRNDGEDHANQITKFHYEYVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBX8</accession>
    <name>A0ABQ5SBX8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010631</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053099">
        <property type="entry name" value="WAS/WASL-interacting_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48226">
        <property type="entry name" value="OS06G0326200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48226:SF1">
        <property type="entry name" value="WAS_WASL-INTERACTING PROTEIN FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="369"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="416"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="591"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="739"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="524"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="416"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="432"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="594"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="635"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="760" mass="77986" checksum="25CB2A347C98E9FF" modified="2025-10-08" version="1">MQCRCRSTPLRMTWVMKSYNKHCLAAVRASPTRFHIPGREARGISVPVAASQPQNIFLASDNNRENPNHLGPNGPKLLPRPIRPKSDGGPGSGKGSGGGGGGSGGGGGQPAGFGERTLASLAAYTGLVGTTALVLGKLVGVDLWAEFDWASLDDLRMAVEVSIPLQLLNAALLLPSYSSPKQLPDFGNLKTLEANLKSERERQEKQKTLAPPASPPSLSGTAVDDAAGAIAAAVRTSTAEPPAAAAASTISPMVPAGLWGRSREDGGLKDNQTGEDGWTSLRSALHLAQGNYISNNPTARLTPAAEAAFTFMDTAASEMLYRGVALTWLTSWLQDRVYEAGMDEMLTYELPAAASTLGLPSARDVLATLGVYGACECAVAVGVAAFVAWLAAARSRRAIQRLEMFMKSSAAASSTARRSGGSAADTDTDTGTGTGAKASGGGEGSGGAARAAAQVLFGPGGPSVGISGAAAVMQRAAAVQGSRDAIQVLLLNGIFIVSGGNLAASYTASVINQLLFSVMQRRGVNRMRERSAALARELKAYNAQMQRIANKYKDKLPPAVVSRLDRITEEEAVAEASAPSSSTAAVTIIKDDTTTGNSTGNSKASSSSSSSSSTASTTSTTSTTSSNGCLEVYGSSTQSTATSSSPSSALPDDINGNVGSSSNKSSSSSIVSSSSIDDSGSSDTSSDSVVSTIARHLTPNAAGSADVLGTPNGTNSIDIDSNGSNSLVRVLTVLDMLVPEPPKPVAADRKPRQQQQQRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SC97</accession>
    <name>A0ABQ5SC97_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_011703</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255">
        <property type="entry name" value="CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255:SF3">
        <property type="entry name" value="SULFITE EXPORTER TAUE_SAFE FAMILY PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="808"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="916"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="953"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="981"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1014"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="427"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="514"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="578"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="618"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="845"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="620"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="852"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="879"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI67501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1059" mass="108390" checksum="5EC7F29E03D916A8" modified="2025-10-08" version="1">MAQQLEVDSPFHADARSLLGIIVAVLIGAVATAAGVGGGAIYIPLFNVLVGFALKPSTALSQACIAAGSLAAVATNLPRTHPSVAAAPLIDFPLVLLLTPLLLVGVGIGVLLNVTLPSWLLNILLLVLLLLLLAQAVAKGKALWVQETKRAQAAAEAMAHRDIDIENSAESPDRTAASGSGMLEALLPAGHRRSFSHPALAAVTCTEPPGLDLRRGHSYSGPRAPVALEADQDVSSGRLAGLENSGMAASHQPSIGGSAGIAGGSSSIRPARRFATVSWDCTENTTGDGTARCETPYSSVSMPPSTLPFAGPIAPSFVGADDVSAEGPTRSIPGGAASSRALSTASSAIYAASWKAFNELGALEFETDLEGDSDFEGAAPVSSVVGRHMRSTRASGFGSGTASPALLPPSSYILSQLPRRLLLAQTQLSSATRSPHNALGRRTTRRRSFDQATGADLPAAASAAMDEEAGLAGNSWLQRRSKWRSRFPPGALPHRGAARAALLPAHAYPGSSSLLPPVQQLPPSPGTALRASGSGVMRLQSRSQRQCDGVVGDAEGGRGGLDEPLLGLQGEVPDRPHGAIPDPRAEYGGGGAAAAGTATAAGGDRVLAVDSYRPPTRLATLHEESDDRAEGGTSGGGSGSQSTQAVSRLPHQLPSMAASTSTASLDQLPALPTSQPAADWDPLRRPSLQPLHATSASLYDRHVGNHGTGGDGTTDAMALRWTAMGTIGYVYIECMSDTEAEKRHLGRGWCCQANFICGAIAAAVRQWADEWAAALSRIPHRFACGLVGAWVVYLVLQGARDFKTQCSPAWWALFAIQIVAMLAISGGAMVMATTALSKHAEAAETSNGGVRVEGREGAPGGGEDRSGSIRRRQQQQQHGHVEEKESHRGGRGLSAAVAVEPALQCTAAMLVLQAPLTTLCATFGAGLTGGLLGLGGGMVMGPLLLQIGVQPQVTAASSGAMVLFSSSAALIQFALLHRLNGAYAGVFAAASLVAGVAGTHAVARAIKRSGRPSIVVLALAGVMGIGTVCVAAFGLHQAANQLRTGELGFAGICNSCGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDG6</accession>
    <name>A0ABQ5SDG6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_012282</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17955">
        <property type="entry name" value="DEADc_DDX49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="10">
        <location>
            <begin position="95"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="126"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="326"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="14"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="95"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI67950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="515" mass="56371" checksum="63243CEF77706DAA" modified="2025-10-08" version="1">MDGDSDVQLFAARAIKKRKLKQEGPPPNKKNSETQKQKRFAATAPTAATQDEPSKKHLERRPDPGIEDNVADNGAAKRSGGVASTSDKEWSDSVADFKRLGLSEWLCGVCKSLGMHTPTEVQRGCIPAILQGRDVIGLAQTGSGKTAAFALPILQTLAKDPYGVFSLVLTPTRELAVQISEQFHALGAGMRLKELVVIGGVDMQQQARDLAKRPHVVVATPGRLRGLLDADSALATCLGRTRFLVLDEADRMLDVTFEDDLRVILSCLPADRQTLLFSATMTRSLIALQKAALQDAHVFKAYEGLRTADRLCEEYFFLPAKVKEVYLHHLLTVVLPERKVRSAIIFTGTCRGCHLLSLLLEELGLPATALHSGKSQKARLAALSCFKSEQVPLLLATDVASRGLDIPTVDLVINFDLPMLARDYVHRVGRTARAGRSGWSLSLVTQYDVQLVHAIEELIGHKLEEHKLDEQEVLKGITKVYSAKRAAMLRAAEEEGLDTSKSRRVKKAAKQLVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDH1</accession>
    <name>A0ABQ5SDH1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_011972</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039772">
        <property type="entry name" value="Bin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010675">
        <property type="entry name" value="Bin3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024160">
        <property type="entry name" value="BIN3_SAM-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315:SF0">
        <property type="entry name" value="7SK SNRNA METHYLPHOSPHATE CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315">
        <property type="entry name" value="BICOID-INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06859">
        <property type="entry name" value="Bin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51515">
        <property type="entry name" value="BIN3_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Bin3-type SAM" evidence="8">
        <location>
            <begin position="591"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="61"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="674"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="679"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="729"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI67704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="976" mass="102848" checksum="134BFA478F591C86" modified="2025-10-08" version="1">MSEAANCGTKAGEHAMPPTDPGTLVQQLKSQLLPSDKIFSKNQRKKARKKVTKIIQKTLGICPRPGVNADGDARTDDEGTPSSQDTGCCSNHRAAVGSGEARPACTSSPQTAKFFRPHSPGASAPPCRSVMEATDAADAPAMYPPSNRRRVGSALNRSSSGAPALGQNRDRLPRATPAVARHAEKAASLGHHGRAERAASGAEGLPTCRRKDARPIARSSRLTGSGSPSIDDNRGGSSAAGGSNQLSQGALKRLSRRQARPDDLLTADNDDNDDDGKATTATAGPSHTPGCLKPGARSVIAVAAAYRSAGIAGGGEMACAAAAAGKRRDRRQSGRSLGKRARLSEDGTAVGAAASEEVIQIPPQAGTAASAAAVAAAATAVHRGYALYCSTFVTATAATSHLKGGSVARQVLVPAASMPPAGGVAAARGPLDLSAALLMMRVQLRASDTAGLGGPVPPVIGTADITSGVDASVPAVLTATADANMASGGTSTIRLSGYASDRQGLEDSGSKAALFTSCALSTGMEGRVAAAPAGVNGRLGLQKTSMQAQKQQPCKVQQQAEWQAAVRASRYRYGNYHRYYGYRMSADMDEDPRVKLMRREWFQQRVCIDVGCNEGLVTLAVAARFGAKRMTGYDIDGTLIKKACRNLRNTRTEYTTRLQAMTHGHCIRPAHETSWGNGWEEEGYGDEMPDDGDDGVGGSDPAARNPMVLTGGKVECGELEGDEGRDADADAVEEEYGSETDCADEILPDAEASADDTVNVDDENGVSGDEAAAVNRNGKDPSCGQVSPPLSLQQQKHKQQQQQHQVLASSRPRRGPHSAIGPAPVRVGMLRSYVKALAGTRFVQQDWANARGRSDSADTILCLSVTKWVHLNNGDEGLKRMFKKFFNTLTPGGLLILEPQPWKSYNRAVHKTTTNAVPYKRIEQLQLRPQHFVEYLTNQLGFVLESQLREGQGVGFDRPMYLLRKPMLVGAGPCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDI7</accession>
    <name>A0ABQ5SDI7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">APAF-1 helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_011767</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041452">
        <property type="entry name" value="APAF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17908">
        <property type="entry name" value="APAF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="APAF-1 helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="506"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="547"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="589"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="680"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="722"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="764"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="806"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="849"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="891"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1152"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1194"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1235"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1277"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI67543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1362" mass="141851" checksum="6DCE279C7420F00A" modified="2025-10-08" version="1">MGCAQSSQSSGGGTAAGGSPGEESSGKHASVAATGRPDAPLDEGAAASSLKKQVRDSVHDINGGTAKATKEDAVDTCKSCVSTCDCRDPERGAGAGDKAILPGSVLPLPSTTTASTVALGGDAVAVDLPASQVSAGGSLDGVRRSSDTIAAVSTPAARNSSVDKLASNRSSFAAEGIPQHSQMATVMAARGSRGGPADGGGNSPLPLMLEVARSSAQARMSSIQAADVMGLGGDKAAMVPRAMLPAYDPLPGFQPPAALANAYPLLGAVPLDTPVPMEVLGRLWRSPSSEATLGTARAFASLGILRLASLEDGSCWALPAPQHVAHAAAVWPDMVTKVHKQLVEAYSRRGTVALDALRDDGYIIQALSHHLVGSGQLDSLRRLLMNPGWLEAKLHAYGVGAVVRDFRRFLQEAVSEEEALGVKLLLQAFQLSLGAAMEHPTARMLREQMLARLMAVAAGGRLKDWFAEQTSKCAAESMLAANSRLLHLMPRSPSLAQAGGVQRMTLRGHAGPIPRVAIAPNCCEVVTISEDGLAQVWDMNIGDCVMHLARSAPLTAVGITPDSSAAVVAAADGTAAVWNLTTGQVIHNFKGHTGRINCLAIDKQGIRVITGSEDHTGRVWNLQNGECEAVLQGHGGASGLVGSVLDVCVCADGTLAVTVSDDFSGRVWDLDEDGEQLHVLEGHGGWVTSAAFVGTSHRVVTTSHDTTARIWDALRGRCLFVLTGHSGRLNRVSVDPGGSWAITASDDRTARVWDCETGELKLVLDGHNAHVTDAAITRDGRKAITVSGDGTCGIWDMDKGKREALLEGHTGEVSAVALTQRGRFAVTAGEDFTARVWDLAAAAERLTPAPTHGGKVSALQSLPDGQLVVSAGDDGRVFLWDPVEGVCLKQLDGHRVGIRFMRVSAEGDCVLTGSGDRQINHWNWAAYSAAASVVGVRRSLERRELMLHTLTTGGSGGGQSLGTKAYTGGAGGGACSTANIAPACGTGSQLFSTGGTVITAGGATNVEGGDRSERSSEEDPMAALAALKPPGSGGNSNAVMAPRITSGSIPQRVVVPQRLQGSTAGSPCTTPSPHGGTLRPCKSLLEMVSTLQHEPLRASMPAQQGSRVKHMAFDAACRTAAVLLFDSTVSVWDVVSGKCTVQLIRRGERDAMRTHSGGVNAVYLTRDGLTAVTISKDNTARVWDVAAATTRFVVDGHTDGLVAADISGDEQLLATAAYDKTVRVTRLGNGTPVAVLDHPQPPTMVCFSPNNRWMAVGLEDHTVVVWDLVNRCCLPSLDAHKGPLAVLTWSSVNRFLLTAGQDCTLRLWRIEDCRQQAFFMGDAAITAACFAGNSVPDIVVAGDSAGTVHFLDFAEELQGWST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SE42</accession>
    <name>A0ABQ5SE42_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011986</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19163">
        <property type="entry name" value="AKR_galDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044479">
        <property type="entry name" value="LGALDH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686:SF1">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="25"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="342" mass="36315" checksum="89B40E085B62F8B5" modified="2025-10-08" version="1">MGDRDSALRLPMRPLGDTGLEVSVLGFGASPLGGTFGAVDEAAGIDAVHHAFRRGINFFDTSPYYGLTKSETVLGRALRDLPRSEVVLSTKVGRYGVDPRDFDFSAKRVTASVAESLERLQVSYIDIIQCHDIEFGNLDQIVHETLPALAALRSRGLIRHVGVTGLPLEAFHYALDRAPPGSVDVVLSYCHYCLNDTTLLGSLPYFRAKGMGIINASPLSMGLLTKGGPPDWHPAPPQLRAAAAAAAAHAATRGWDIASLGLGFSLRQPDVSSTLVGMFTREEVDENTNTALRVLGATEVAADEAQVLAEVADILAPVRDLTWPSGRPLPGQGRAGSPPADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEX1</accession>
    <name>A0ABQ5SEX1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TraB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14726">
        <property type="entry name" value="TraB_PrgY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PHEROMONE SHUTDOWN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530:SF0">
        <property type="entry name" value="TRAB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="51152" checksum="7CFD0856B57F8CF4" modified="2025-10-08" version="1">MDFFVRLRSTSGCAPRLALAQHSRRPRIDLAYLYSDALIRETRFSAVVSEQGSTSTYDAELQRLLLAGPPKDFDFRAEVMTYSRQWVDDNACQLRDLVDEGTVVVVPRRPDYKERRTDGYCEPRVVVLVGTAHVSRRSQRDVDRVIRAVVPDSVVVELCKSRSAALMATGDLPPSVPSVAGAGWAAEGGNTTSVGSVRGSDSDSDIGFSDAARLRDGVAASSIGSSPTPPAYVNPMNLAGGGDDGSGRGGGAPPGVGFLGAVARSVSLGGQSALLLRVLLAGLASRTAGSLGVAGGGEFAAARAAAEDVGAQVVLGDRPVEITLSRAWAAMSLSRRLKLCGELLRGALGAPRQALSEELVEKLKSDDAVSAFFSELSNSYPELVAPLVTERDLYLAWSLKRSKAVNGAGVVVGVVGRGHLHGVVYALLRDQGGAGLRFSDLVEGRNNRKRRGEKAVQGLQRLFIELVLGTAAYFAWLAFQQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF07</accession>
    <name>A0ABQ5SF07_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012254</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047361035" evidence="3">
        <location>
            <begin position="30"/>
            <end position="3411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2620"/>
            <end position="2642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2663"/>
            <end position="2682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2725"/>
            <end position="2749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3093"/>
            <end position="3116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3157"/>
            <end position="3184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3208"/>
            <end position="3228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3234"/>
            <end position="3254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3266"/>
            <end position="3286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3298"/>
            <end position="3319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2817"/>
            <end position="2846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3369"/>
            <end position="3401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2823"/>
            <end position="2839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3386"/>
            <end position="3396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3411" mass="356023" checksum="A482C357D3A9780E" modified="2025-10-08" version="1">MLLGSKRREIWKLLLLHLAIGLSPRPAFSVLTCLFSFWGNSDIIDFPRQLDAGSICRCSASPSGEGQVVVYFAGGNQDLGQLCNSRSGGELLGPTGNLPGLEAATVLPWADATSPSATSLRFIGGRDLVVDGALAGTPTATNLDLGYNGPAMPLLYFNQVNGLVLRNLVFSKLYVSGPLILIEDCTNVTMQNVTMADVIISGESSGVPAPSGAPTSPLLGVAGIITPLNEPDGHDVSPAVYGGGSPPNGGEFVDPPAPDQSDNDGTVASMYTAAIWLKNVSMARLEGISIKSFSASFASFTTAFSYLNGLLISGSQSISATNLYVRNFNAEHSFGPSGTETTSAVLVLESTLATFSGLECSFSTGLCGPCLSVIDSTVQVTDSDFINNVAVNGSGGGAVCVQSSQSPHVETRMQFERSSFSGNTANSGGAIALVSFNAGAPTLKVSSCAFDANSATNMGGDIYLCDASQISVDSSSFRFSSVNGNISDNGGCIYGGSGVHASFTNSTFEGCVTTGNGGALYFMNVVTSESSLGSPQASITLEECRFSNNSCAGYGGGLAVVTDVDPSHGEQLRMNVTITNSIFTDHSAAEGGALWLSNEQFAVQCQDREDPGTNSAMAAVRLLNVTFTNNTVMYRGGALLVDKWSFQLDGGEVSQNSASIDDGASIGGAISLRDCPGTAQVSNVRMITNTGLYGGALGINWCSLKIWNAYIADNNCSSEGGGMVATCYGVHSTTTRNYTMYTVDMYNVTFKGNIAGSGGGGLAASCGNLTVVDSWFANNNASGDTGGGMKIESPDQGSNRTLPFTVLRRVHLANNTAKMKGGGIYMIQTGAIVEDTTFENNNQTNDCSDVITPCAGGAIYISNGRGLIKIHNVTVTGNIASQGGGMVLDMTNATIWDSGFSGNTATYDGGALLALQSGSNAEDVAYAVELLDCVFEDNVAGNHGGALTANRSLFSAKDVVFQGNKALESGRGGAVFLTCPYLPLLADVPWSEILTTDNTTVAKVDHCQFLGNSAGDSGGALYYTASVVHIFSCQFLNNTAAGADGNRGAWDSSGGAIHGVHSPTLLVDSILSYNKANGRGGALALQSCMVTVDGSHIAYNVATFSGGAVAVGRNVLSENVEDFPLYLVNSNLTNNRAEMEHGGCLFVDALPVGILNCRLHYNEAKLLGGAVYLASSSNLTVRSSALQGNEAEIAGGAVYAKAATGEQTSVATLYGVRFQDNYARAAGGALWLSGWQVDMEDLDMITNSAGTFIVAGSDNSGGAAYIADCVGPANLSDARIWGNAAYQGGAFYVELCSMSVQNTIFEANSAVGGDVGALAAIGFTSSSSISSNRTFALDLRNNTFQNNTAGGRSGALKAYFINIKIVDTHFLDNYASLEGGAVHLQVVPPSLQYGDQENQVAEKVLIEGCEFRRNTVSNSGGALYHVTSAVRLRNCVFSENIAGNGVGSTVSSSASVALGISTAGGAVFATSCTAAMEVTNVKMDSNQADGQGGGMSLLSCSTQFENSTLTDNVAKLSGGGVAVGHQVTISQLEAPSVTFHNCNLTRNKGYTQDGGSIFTTSVHLTIYACQFLDNEALRNGGAVYSMAAPSILISDSVSIGNRAGVSGGAVWVESATFVQVNGSALLENVAGVSGGAIWLSNAHCTHLEAVTFINNTASEGGGVYVTMRAFTVTPEVENLICMDGFQRQLGDSWVQRLSQESQSVIHSKRHELVAVVTNVTAADNSASSGRGGAALFVATRGSILLDAFNASYNHAGDTGGGIAISDSRLVGATVYLSSSEMANNFATIRGGAVSLDGGRTNLQFVALGSRMQFNTAGLGGAISLQLNASAALHGNILDGNIADDGRDGGTGSGGSVHALSCNWLLVNSTSIQSSFAGRGHGGGIYTDGCKVVMLSDVGLRNNYATGAGGAVFLSASSTTNADGTSLAVISRGDMVKNLAGVGLSPSSGSSSVATASLAGGSAGALFVGGRMVVLVDKTTFDGNMAEAEGGAVGVGLECLQAPDTYTGPLLESLFISSESTNYSWRQEDIGGLNVGVRELPTAANPTLQALERAANRSAAYSCWAAIFNQPRFWGNTAGNSGGAVFSTSPYTLSIICGAADRQSAAAAAGFSKAEASDQGELAGLIDTTTTRVASSSISNGVFDLATAHTETVSNNTANMTLLPYMGLMPVSDAAHKAVCFLRPASLGLEADSPQANNARRGYGDEVATAPKFVALVALDSNDAGSTYMENKVTVLGSDESVAAYMSIGSNDGTGGLGVVNGSGASSSSSSSSSSQWARWSSRAVLLVSGRRGNKCSPGQDCILTVPNNVIIGLNVSIFDAANQVANDTGMLRSVVRATIYSTDPSRRADLLGNPLAQVVEGSASLAGIRVRAYKGEYVLKLMLDTNTGFQVSPLVLNVTVPPCAIGELPLGSGSDCSECTLNTYSLTVDAYDDSDTSINSRNFACKSCPLNAICTGGAVLVPEQGYWHSAANSTVIHQCPNLYACRTGDDEANDELVRCQERWYAYFGEARRFIKLVQDRTGGTELVGRYLEAYRNVKSAGNNSYQGALISSPLSLDGAFDGFLLDCALWGVSDDDPLSYMQKQCTQGYSGILCATCTRVDGKDYAMLSDLSCSPCFPTVATIFIGFAIFIANTLMLGASVLMTFLTDYTAGHDPNTLPAADLLKVLVIHYQIFLIVTRIHMNWPKSVMGIQYVVASVTGSVKQAYSPSCLFPGADSSEQATVQVLAGLLLPICSTVLVMILWIVRYFYWNQRDPRKMLTHIRSQSTSASTHMDAAGLNNVGSSGKSADATGFRSFPKSSRSLLQNTSSVASAACTTDGGDGNISSNTSGIPPTISRTSLGGGRAPKELCTASSTGSFVLPPDMLPTVAIVLGGGVQVDGTPSAAKNLAQLTQVAPPAAIPSVPAAILQRPAAPAYDDDIPLRSAFAIAQVSFAASCRKQANDFSANKPEVGRQAAAAAASLPGQKPLLLSESVPENSCFLNPQSSGALLGADNHGGAVLRKTLSLRPAASISQLPQAGADRGLQHCHSLDSNLSLLPSAFFGGHLPLSTVDSSEGARLKPTKDSQQLGLFSMFSTRESLSFVDSNLPLMPQLFLVLMVASFVLMPTWATEALSIFSCYKLDNLSGAYSENQKATWPYGYWVRDMNQECYTGRHQTFWLPIGVVSVLLLCLTMPITSAAITLYFRKRLNEVEVLQVYGFLYRTYKPKFFFWESVSQAQTLLLVVVEVFGRTMWVFNQALLFEVFLICILMLNTYVSPLLYEQLTALQRLSLGVICLTVILGLFTSVPTAQVSEVGQDVLGAIILVINCMVFIGFLVLFIRYVMPNLKVKVKEVASQLRTSISRLTSERSATWLRFSSRLKLSFTYSSRRNEDTSGVDAGGSRHQRASSSSPPTGSAGDNGMPILSAVSIHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFC4</accession>
    <name>A0ABQ5SFC4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI68188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="314" mass="32682" checksum="6CE2F66CDD4499A8" modified="2025-10-08" version="1" fragment="single">STVGGPPRACGHQQQQQQAGDLLVADARTGIPATAVPATPVPAAATSFESSISDKLWDIYMGSEFGRAEDLLVTQSQVNQLAPAPVGEMPFPPLQPPPLYQPEYPTTAAAQPPLLASFLGPVSEPAVPAMPLIQGLLLPPGLLPPAPMPHAPLPNPLLPSQLLPSALPPALLSAPPPGMLSGLLPPPLLLPQVPSLPLIFASSLPPLPLFGTAPPVAAMPTSYAAGLPILDFGLENIDAKAQKMLTKLRLLNHPRMRDLIMAVAACKKLGARTPDARMKIDSACGKVLRDLAVVRTLALQSGMLACFGRDPGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFP7</accession>
    <name>A0ABQ5SFP7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_012507</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12850">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="108" mass="11994" checksum="D1A4F5923488BE62" modified="2025-10-08" version="1" fragment="single">KMAPKEQKSKEAKALAAANSSKGKRKKWSKGKMKEKVNNAVLFDKAVYEKLLNEVPKYKMISPSVLADRLRINGSLARAAIRELANKGLIKPIVHHHSQLVYTRATGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SG38</accession>
    <name>A0ABQ5SG38_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycoside hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_013344</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF36">
        <property type="entry name" value="LP05116P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="487"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI68922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="57356" checksum="5E5282A1AA528C6E" modified="2025-10-08" version="1">MNGNAPQISPDSNEQTDKIRFLKGSAISIWQNSGDGGSNWTRFANSLWPFRYFGVKAIRGKYNIDTCSDFWNNYERDIKLAADIGSTTLRFSFEWARIEPERGVIDMEAVRRYHQMLDCMAAHGLEPNATLWHFVHPTWFEDAGGFTKEENIPAFVEYCKRCFRWFGTRIRLWATFNEPTCYMFLGFIVGIAPPGRIFDLVAAGRMLSTMLKAHVAAYKAIKAMPGGDKAQVGLVSHHITFEAQADGILHGVAKLLSDWMTYWWGWDVLEHWMLTGEFVWKLPVLGVWQQWRDPEGKPPCDWWGINYYSRGIFSWYLVPSCRQQEVMTDMYYPIYPEGMYRAIKRCSEFGIPMYITETGIADARDDRRAIMIDAYFKEVMRAVAEGYDVRGFYYWTLLDNLEWATGYTMKFGLYAWEPDGSVDRKLKEGSKTLVRYFRTLPDSLEEVREAARKAQAVLGADLGVEQELKEEGFVRMGSEGKLLPDGKGPGEPGGVGMVAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SH41</accession>
    <name>A0ABQ5SH41_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Plasma membrane fusion protein PRM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_013848</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tweety family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006990">
        <property type="entry name" value="Tweety"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424:SF8">
        <property type="entry name" value="PROTEIN TWEETY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424">
        <property type="entry name" value="TWEETY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0868">Chloride</keyword>
    <keyword evidence="9" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="838"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="871"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="655"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="727"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="731"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI69272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1191" mass="126342" checksum="029FA6EB49F511C7" modified="2025-10-08" version="1">MGSAAESCPSALWQKLPFLPEPPTPGFNLLGWSKAQYLSYAKATILSATAVNVAFLGIVVVLFLFLLVWRLLGGICLCCSVCCGVKARMPRRKSLLSFEVMHDPWLLGHKVAFAFFAFGCIVLAITGLATLPQLRQQIDELASRLEEVSNYASSLLATSDGLLGSASSLRPIMSQVLTLTQPQMASKSLLSDMECSSAFLDAGLVQAESLRSQIATFISSWEQFTPLISTAAALLNITITPAPLGATPSMEVVSNIRVLLIGIDDALEAYNTALTALPESRQPTSVSDPQVNALTAAQNTVNITQWITSLDLLSESMKTLDVAAQNLLYSRLGSTFREVLIFSVSLNSKVNTILSNLDGLDRLYMSGQRPPPSPQQQQSTVAGRACLLSMVQQLLSFDTSIFRFNSTLERNALDTIYNVTSTLEQIYRGDPVGISTDAGAGATNALIGNGTVVPTELARPAWWASQYLQPLGNVDGPGFANQCYHVQSMLTDENNRTKVPDLTPILSHISILRSTLVGLQRPFSGVQAALVAYNKLASEVLYSSLIAAVNTTKPALGSALNVSSPAFPGYGPGLWATAWNGWGAVNDVPRGGLSVDERLRLMSKGVFGLNKWMTEDVNGLVGLKRTLAGLSAIGSSVNQAQTVLVRSGLANTSNSQIGINSTVTGGGGSNTSAPSSPPPASPASPAALRKSAFLRPMVPSMQIITSTLSDGVASVAEQLDNALGKALLLISPPPPPPPPMAVDSPPPPAPSPPPPRPALLLSLQPTTSPLQGNEISIPFVVRPLRQLQQSAGAFTSPDGANITTVVYRQLVKPLNELNATLRGKTGQRYLRNVYSSAVAVYSCLIVVCLLMGVALFFNFPAGLVIGLGVHLLLATLVLAGAWAAAMGMVASHDACGNFDSMVLDAVSPSSSLFPLLRYYLQGVGGSLTSILRTTGLGDIVRLKSAVNLVRTEVLGPLLLPRDSNRTAGAANSTRPWPSSIGTVQYALGELLVDLDAMAANVADQLTALLTVADRANVLHQYGIITWWPCCSLGEQLLVAWISATGCGLLAWLAALSTLLVLKNLDDMPVGSRWGCTCYDPRDFPQPVRRRRYKCSAGGGEAYSIHGYQGGSASSIGLPPMPTVQMLAPCPLRSPGGMQRSYSGRTMPSIRSQDIRPSYDGYSLANARVECIMKITSEYDTAGAPRELAVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHF7</accession>
    <name>A0ABQ5SHF7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UVR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013382</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI68839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="219"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="394"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="634"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="312"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="403"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI68839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="716" mass="75901" checksum="8E32E06D38AFD192" modified="2025-10-08" version="1">MLCSWFSNSWRAPPSASSTVGLPGVPSAATGILRRHGRSAEAPRAAGFVASGASGAGLAPQPVTWRSCRSSRQKHNAGVFLGDKLVAAAQRARATSGKPSKDQVPPETIAKPADEGPSREGVQQQRQLQQQRRYIGPESLPSPSQPTDIGFQSSGGSNRSLSTGDTARSTETAGIDATPKRRGRPPKRHVEPVAATQPPPTATVPLAEAAAAVAAAALSNTFDSSSSSNSNSNGGRSRRPFGPEPPPPPNQRQQAAAAVSPDNISNAPFPFSLPDDPSQLNVDDILNLPLPDPRFGEPFRLGGDLSELADLGEAAGVGAPGGDAAITGKDDDEDPDLDYDELIALAGKVDVASYVARAFERAKQGLGPVRGPELADIREVEPGGSAVSQNAVCSQQTMGPATAAPPRPSPPQQQMPSSSNTVAYADGRRRTDASPTAAPQAPGVQRPPSSHDNAMYVDEDVEDVNEAVADRFEAKAAAQRRVALLVELLAASGAELEAKLEAAAPEVDEQLLALLERRYTTALALRQEAASAEHIATLYRALRYIYERRTSSAAERLLDDVLGILGDVQLQPDIERRRGEAAARLRNAFTGGMLDVDVFTAAAALAEGRQAAAEALAGEQVSLETFKTEAMGLLDHAKRVQQKTEANLETIEAEVARLRKEEPNTLESREWRFRLDQVRLARQALTEREESITALEEVLVLTRAVELQMLTGRLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SHK6</accession>
    <name>A0ABQ5SHK6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RecA family profile 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_013543</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RecA family. RAD51 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19489">
        <property type="entry name" value="Rad51D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051988">
        <property type="entry name" value="HRR_RAD51_Paralog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047323">
        <property type="entry name" value="Rad51D_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46457">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46457:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="10">
        <location>
            <begin position="104"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI69005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="423" mass="44903" checksum="62E5DDE6EE83CB3E" modified="2025-10-08" version="1">MTCKHFQTLRESEMRWEDLSGVLPQGTLYNLACNYQSVEAFVVNRSKALSGTTGHGILKGCVGPMDVSSDVDAVDLAVIEPLLDSCLAPQWRSGAQLMHELRYSIQVLPTGCSAIDTLLGGGLREGTLVEIAGETCSGKTQLCLSATATTAYRGEAVMYVDTTGSFAPERVVQMAEAEAAASAGTWPQLQAPDAELATRILRCISVLRPSSVYDLLSSLDRLGAVLERQLQEQEQRQQHAGLQEVQGVGGCMSQSRDGQVEAGDVLHPHRMRPRLLVIDSVSALLSTVLGNNQHMQGTALLTAVGRILKQLAQQYSIAVLVTNHVTSANSLARGLGRGLKGAGSGGEGAAMDRCISTSATVGVGGLKPALGEQWRSQPHMRVQLSMDASQAPESRVRVATLTASPLQACGQKLEFRIAATGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SI18</accession>
    <name>A0ABQ5SI18_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_014259</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="11"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF63">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI69597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="327" mass="36154" checksum="1D4F660A837D59AE" modified="2025-10-08" version="1">MPAQDLLHKFFDAALEFIAVGIYVSSIYTAGLLLPGVISAFVLLGPTSAVPWVAGAFLVILTFTPLPLCTGRFSERFIQFSCRRAAAYFPLSVVCEDAGAFLSDRGYVFGFCPHSALPVALPAAFATNSQLLPKALRGRTHGLASSVCFQVPIVRQLYWWLGVRPATRSVMQQLLAQRRIAVLVPGGVKEVLKMEHGIEVAYLSTRKGFIRIAVQCGAPIVPVWAFGQTRAYSWVRPGPPLVPSWIVARISRMFGAVPIYMFGAYGTSMPHRERITVVIGRPIPVQQVDDPSEEVVSELLRKFMDDLQALYDKHKDEYGRGEELHIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIG8</accession>
    <name>A0ABQ5SIG8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-L-isoaspartate(D-aspartate) O-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.77</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_014447</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI69765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="209" mass="22398" checksum="E2D38793C414DA94" modified="2025-10-08" version="1" fragment="single">MLNRNTIPHTRRLPSIAGLRLLAIVKTLKTLSCQSTRTLRRRTLMTTILAGHCASPGMHRLLTKLIDHGVLQTPAVAKVMSEVDRAAFVTPMWANSLRAYEDTPLPIGHGQTISAPHMHATALELLNPQLRQGARVLDVGSGSGYLTACFGLMVSPGGRVLGVEVVPELVEQSVQSLRRVVPVLIQDGTITIEAGNVHSAAGVLASEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIL5</accession>
    <name>A0ABQ5SIL5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_013906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045475231" evidence="1">
        <location>
            <begin position="19"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="500" mass="55757" checksum="A6B949AF18A5E517" modified="2025-10-08" version="1">MGSHVPIAILTFLLLTSSAPCGSLQQLFYNLTGNFSQSGYELREIYNVSKELIQQRISVHKDAEAEEFGRSLDAICNKVTKDNFLQSMRASMGLLRRVAERDTIIAILGRRLVLVCLTLRRVMLKTELSEYDGETLFSDVAKELYAIHVESVIRKTLVDYLHISKSGGTSFSQAARLSKCKMGGGIGQPLYIGDLPRWLNRTVFQNVTNGVHMTWSLYGKIERGKVSGCERRIGNVSMQGMQYLSNEYTLHGGMNGMEGVHLCQQVVNVVTLRQAEARLVSHVHFLLAHIKVRLARSHRSTGLDLFQEKFCTNNVSMWEGLIPAILDNYNVRSFIGERAFHTTIWGIGRSHLELARRLLLQFDLVLDLDAGDAATDLLMYQGLGWNVTLAEVHARTADVVSSRTNYSRTECAIEKLLAEVLPRQGPDKELYSLGRTLSLLDQLLLGWAQEIGLRPEPGTSNATTVVPPTKTEPPIHCGLLDKKYRGAKVQPDGGAKNEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIQ6</accession>
    <name>A0ABQ5SIQ6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Molybdate-anion transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Major facilitator superfamily domain-containing protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Molybdate transporter 2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">VaNZ11_013961</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mediates high-affinity intracellular uptake of the rare oligo-element molybdenum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17487">
        <property type="entry name" value="MFS_MFSD5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008509">
        <property type="entry name" value="MOT2/MFSD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23516:SF1">
        <property type="entry name" value="MOLYBDATE-ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23516">
        <property type="entry name" value="SAM (S-ADENOSYL METHIONINE) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05631">
        <property type="entry name" value="MFS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="GLI69368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="507" mass="55245" checksum="CF410D5F330C7D3D" modified="2025-10-08" version="1">MESVYWFIFGALAFLVAGLVFTQVALFKRCWALTHVSCSCMTKEGGIVSGNAAQLQAFFSLRNNYVFVYALMMAGDWLQGPYVYALYQHYGYDVKDIGRLFIAGFGSSMIFGTVVGSLADKHGRKKAALIYVLAYAASCATKHSPEYGILMLGRLLGGIATSLLFSAFESWLVAEHFARGFEEKWLGDTFSKAVFVGNGLMAILAGLVASYLVDTLKLGPVAPFDAAIVVLVAGGAVIYTSWPENFGDSAHAAEGVLQTLQRQFAVATGSILGDQRIALLGAMQSLFEASMYTFVFLWTPALSPAGEKIYHGMIFACFMTASMAGSSLSGILMKRYKVEAYMKYVFGLSALALSVPFLFNVSIAKDSRRSLQDATLTEGREAVRGISLHAQIQLVAFCVFEVLVGIFWPSMMTLRARFIPEETRSTIINMFRIPLNLFVCIILYNVHLFPLSAMFALCSTFLAIAALLQIRLERIIRDRPTAYAKGEEAAALLSGTASEADSAKDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJU2</accession>
    <name>A0ABQ5SJU2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BPL/LPL catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_014788</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the biotin--protein ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI70165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="3">
        <location>
            <begin position="144"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="451" mass="48188" checksum="2F1B6A155E07C1EA" modified="2025-10-08" version="1">MPTLNLSPLRYQPHLPPSWGSLLWTSLYVPRHQLIPFAPTTRANEACGSALHPVLSSSSRAVGGSFLPRGTATQASPPSSVMARAEDDKLHIFAFCRTTDDAAKVQEAAKEVAVAASLGSTTPILHLGLADAPPCDAPTLDAARCCQHLAQLVSRGGTRTMGRVLLAAATLGSTQELLRTLGSILGDGAVVVADCQTSGKGRGGNQWTSPVGCLMFSAVRRLRVASPVQAPFINYLVCLAVTRGVRKALQESGLPALDLRIKWPNDIYAAGLKVAGALIHTTWQGGWFNVIAGIGLNVNNRTPTTCLDELLEQAASERTKCAGETDGNGFVRREANEPQTYISRESVLTSILASLEEVYDTFEQHGFAPLEPEYLATWLHSGQVLDVDELDTTTSVVGATNASRRVTRVTIRGLSSSGFLLAEDAGGSWYELTPDGNSLDMMQGLIRRKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJV4</accession>
    <name>A0ABQ5SJV4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_014089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF137">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI69471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="53154" checksum="05928F700CC4AF8D" modified="2025-10-08" version="1" fragment="single">MQAVKLPPWRTTVWPSHVLAGFRTPFIHFWHVHEVDFTLPLVLDRTKRPKAARSAFCICRSVAATQSSSSIRALPDSLNPTAFDSDALAKAAGVQCLVHCRPTPLGRGLVAPHSLERQAIVSVPLQNTLVITDEPLTGISVFGDRCQGLWQQHHGQLPQQLLDFLTGDARWDVRMTAWLLWVASELPDSPVWGPYLSSLPLVDEVTCLLNYGPHEARELQFKELVEEARSQHKWALTVHQSYFGMEGELRRLRLAASLEDTLWAMSMVRTRTFSEDVNGEGLTLMVPYADMANHSFQHNATFCMARDNKRFELRLLFSLVTGEEATICYGEGKPNFEVMRDYGFIVPGNPNDRITLSNQDTLPPLNGASLLEALGLKGDWRNGGKLTRVLPEASSEGKSANLLALARQKNAVLSMNLSDGFPSPKAGRLFGGWPGATSWTDAGRPPPQRLNRNTATITTERAAVDIVRQSYQAALDELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SK66</accession>
    <name>A0ABQ5SK66_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="28">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="28" type="ORF">VaNZ11_015160</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28 29">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="28">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF8">
        <property type="entry name" value="DYNEINS HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="11 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0243">Dynein</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain tail" evidence="18">
        <location>
            <begin position="198"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain linker" evidence="19">
        <location>
            <begin position="1334"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="20">
        <location>
            <begin position="1877"/>
            <end position="2205"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="24">
        <location>
            <begin position="2361"/>
            <end position="2483"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain 3 AAA+ lid" evidence="25">
        <location>
            <begin position="2697"/>
            <end position="2790"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="22">
        <location>
            <begin position="2845"/>
            <end position="3104"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="21">
        <location>
            <begin position="3117"/>
            <end position="3459"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="23">
        <location>
            <begin position="3485"/>
            <end position="3705"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="17">
        <location>
            <begin position="3962"/>
            <end position="4082"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="26">
        <location>
            <begin position="4114"/>
            <end position="4259"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="27">
        <location>
            <begin position="4266"/>
            <end position="4561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="3102"/>
            <end position="3153"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="3339"/>
            <end position="3380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="3653"/>
            <end position="3715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF17857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="GLI70380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="4564" mass="519814" checksum="F9B5A8295896906C" modified="2025-10-08" version="1">[encoded data, 4564 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKG5</accession>
    <name>A0ABQ5SKG5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015206</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007306">
        <property type="entry name" value="Rit1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033421">
        <property type="entry name" value="Rit1_DUSP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033449">
        <property type="entry name" value="Rit1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31811">
        <property type="entry name" value="TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31811:SF0">
        <property type="entry name" value="TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04179">
        <property type="entry name" value="Init_tRNA_PT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17184">
        <property type="entry name" value="Rit1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rit1 N-terminal" evidence="3">
        <location>
            <begin position="734"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="Rit1 DUSP-like" evidence="2">
        <location>
            <begin position="1206"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="697"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="822"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1168"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="558"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="697"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1170"/>
            <end position="1187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1355" mass="140614" checksum="B59E08C9A65883FB" modified="2025-10-08" version="1">MTKTIPIWTAVVNRAVARKRAEAAARLAASGRAATEEPQPSVRHQSQRDDVASHNEPTLSVGPSALRSMQQNGGGFSCTELSPPFHSPWAACAATDYDTSGACARVGSLSQWLQSPVAAAAGADDSESYPAFPYSTPFSRSFPPQTPLQFPSGGIVGSSNAAFPTAVDLSGCRAAGVAPASGSCEGVVDPRNSCDTDLSGMEIFGEVSFGDISDTSEAVLPHRDRCQLQTSMVCCQHEKPVPDADGARCGSAQSSDSSAGTAVVMKLAKAAPAECSAAPGVIAPFVAACPASAALPATSEVVEVPPSMTEIEIEVPVLSALDGAVDIQAAVAEVAEAPSEADVVVELSAGMADVAAQPVGNDVVELPAVSTPAGGGGGSTEAALPPALVDSIECTDSVDLGAASASHVSCSPEECWRWGCMFAGVVDDGNLRSHCGCDTWECPTGGHIDNSELNHICKLNRSSNHDVLDSKTIQMHTGGRTQVKVEEKAQAQSHIEEEVHVKQTGLSPSPPQRQRCPLPQHKQQRPLPSYQRQQTLQHESDLDADGSAGSCSDTCGPTGLSGLASTRSTSSSSSSLSTSRSSSCSSCHSDGAPGAGARVASGAGSRTVRACLDVQPSIPRCRPFPRNHTCSASSAPHWLAGKARWCGSLPPRPSAVSCGGGANGGRLGSSGGGSSCSSHSTCSASCSAPSSNACSHASSSNSSRNSSVGGGGAADVGTSGRDGADADDGDGWDVGLHLPLWVSSNERAQIERRLDGWVDALLRVGADVEGLSKVLRKPLRPLWISQSSTIWVDQVAQPDQLPFTPLYLVSASQPHSYLRQAGGVGGAGSDADGDNSDGCHAGASAAQHHRNKQNQNEQQPHAWTYVYVPGAGDDEESWAAGLTPPVFWAHYTELLQAGPTGVHKSVRQILQREANALAERGTNSGILAEAVMATSTPLCTAPTDQHHLLPAGCRGGGGALLPRPGGQFFIGPTGFALGDLASGAAPGCWEQVDAVLSCGIHEHPSMVGEPRWQYGGLAEGLEAVVEAESDTMEEHGVAAAAVAAAAAIGVAAGARPGQPHARDIAAHATDVEGANAGAAIGQAPLEGIVSGPPELATVETSRCEVVEEPERKAEGIPGEFSFLPPSLGCVDGPAPPDQAARCLTVRSKGGGNDTAHDPLGGVAVVGQRKQQQRSHRHHHHHRHHHNHQHDQDQHHHARHHRPTPRYLHLPVQHFKHSRTSLIQHLDAALGFLSYHLSRGRRVLIHDTNGSDTCVCIAVALLLACYGPCYTGGPSAPAWKRPYCTSDEVGISRGRELVPRGITKDAVRQCLAYVSEFYPNARPTRGMLRQVFNHFVRAQGGGHGEDAAGAVKEGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKH8</accession>
    <name>A0ABQ5SKH8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015409</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="563" mass="56304" checksum="ED79FA22A56B863C" modified="2025-10-08" version="1" fragment="single">MASDEDLSSIDPSRLTASHPRALIAPGTQGLTLHLDGGVAAMAGGHHGTLVPSLGLQNSHVSSYSAAVGPGAAVTQQPLVLQLQPPPAHTGVGSDGTIPSPSVLMQQMLHLQQQQQQQQRRQQQQQYSGSVVPGGVCSAVQQPQMLLMSSQSQQQPTLLGVTASAQQFQPPAAATVGGSGCSGVRGGAVAAVPSATLPPVATSSAASASGGGLPGGTEEEDDASTRSLLLTALQLTSSLTPQQQGPAAEQLRKLPVFFDPLELLAAGQGGPVEGSSTAAMHHADILQKSIRSLRDAQLAQNLSALAAMPADQLKELLRTQNVDVREVIAKLNAHIDLEKAKLEFGGNYWYGSRKSYNRFVRGCAEAAIRENQVSLRQKQQQIHPAATAVVHLGGARATDVDMADADGDSRAAELYGKTTLSLGQPASAAPHGASDAADANGPAAVALANGCPAPASDVAANGPAAPAPAAATSTTAAAAAVMLLQQQLRAAGFDPATAAALAARAAAASTAGPAAGSAAVQALLSQGTQLLAGAGATAATTAAALAAAAAAGYPLATGAMFPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SL71</accession>
    <name>A0ABQ5SL71_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_015746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI70722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="419" mass="44024" checksum="229CEC51A6B74D2E" modified="2025-10-08" version="1" fragment="single">AYPVAGAAIKKKALPPPVEEPPPDMLYYFPPVGAGEEYPPPAVSTKSPPSPCSSSSPQCTPAAELGHAVTTNPSTTPPQSSRNSRLLPPPPPSLTSPVLPQPALPPFPEPRQRLLPPQPSQSPLNPSPPSPPPSPSFLSVSPASPDVTEVSPSRESSPGELPQSPLYAVSPSLDPSSGPLHPSPTEQLQPPTKTLQLPPTETLQPPPETLQPLPEWPHPIDAFPLPFTRPSPAGDLVLPLPHQQIATPPLEPGPPPVPTPSHLQSTPAPSSQGPMAYLMPRAAPPIYLTPLHASSPPPPQPPIYLLPASSHTFHPSHSRTSPGLHDGDHPGSGPSPFLNPTPGVYGTDAYNAYLAPGHVVIRDSPTSGDRLGPILPEHSWNESGDVSGCVSGASNDDADDARGDPEPKPLRGRRLLKWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLE5</accession>
    <name>A0ABQ5SLE5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF6">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="3">
        <location>
            <begin position="12"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="250"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="338" mass="36904" checksum="6CF2209531B02A80" modified="2025-10-08" version="1">MIVSKDSPIIDRQRVLCIHSFRTSGKIFQQQLQHAGLLEALSDLVEFVFVDAPHPASGSIPRDVRPYFEGPYYEWFTAESVDDRVEFDEAKMKASERFLMALLAQQGPFDGLMGFSQGAAMSGALVALQRSGLRAELSTLRPLRFCILFGGLKSRHPDHLAAFEVLSGKVPCPSLHVFGDKDALKNPHCIELADSFRNATVLLHQRGHVIPVLHEPQLAVMRAFIQAVASAPEPPPDAHIPAPLPLARCREQQQPEQQGRKQPQESRQLLRQPTAAVLPAVAAIGRGPERIGCEGEPVGKGANLRRVVGQDDGAEVVEAGSSSACSGLQRQGHMLCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLU6</accession>
    <name>A0ABQ5SLU6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="358"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="947" mass="98829" checksum="5E3EFD55CF252544" modified="2025-10-08" version="1" fragment="single">GGGDAKSTTTADAGADAHLAGSKRRKKGSRASKDKDQLAADAGAKSALTSSVSPTDPAAAVNGHSKKPRKEKKQTAAAALDVQAGPSKSAKDRETETGAPQATSQPEDAVTTQSVATRTETAALKASAPPVHIAEERIGAAVAPGPELPGERQEGTTGLPSAATNPAGPGTNAIGIQSFKESGAVALQPAAAPVIAEEAGRYFPETGTTPVNAFLPERLPRPEETNAQEKRAIIELQRQLSELQVMYKDLKATKIGQMDKLLAEMAERAEGRVCVAEQLARHWEGVAKRAQEEAIAAGSVETANRIDALEAEANQLLKQNQNLLQRVTQLQLELCHAQQDLHDARAELLQFRTSTVRDLQGNAKDKDKDPDTALPAGATAETEHVKDVNGAPRPVAEAAAQESLPEVTLGGVRSYAITDAAATGGRGDGGRCSMSFGIGLQSEMLSPGAGEQTTKINVQLFPVQGPSVVPGSSSRSVGISTDCQRIVQMLPSIRSPSRSIQSHRGRLSRPSSVAPSFDIDLLGGVPRAGSLSSERGIDTACQRVIQMLPELRQPSPSIEPSGVTPATADGNGASLRVQEGGSKRLSDGSPIDGICGSANRKTTCVSTLADLTHAHNMCAPEALGSTANQQGQYSFVDLNPSPARKASHMGVAASIGGLAAAATMGVTKRGGDQPSGAGERQAGPIQGASLLVTAAAAKGLPPSPGLQQQADYAEDVSPHAIPVAATITHSGSVASNQVSCNISGLVARALAQPQEAQQAVGMQEVGRAPQACGDRSPMLANMALAALPSHAMAVAGVGDPMLQGGNTRELSRGDNNIISSPSPRTAKLNFYERLLEWHVDVISTKPLEAFRMTHMGCGMSFEIRQTEVDDDDLAEDVVDADQPADVHDGNTANDKTKYYEYIPLDLGTVNELLPAFLKETITIPADQRPKLLEKLDRAIKSTRGKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLY7</accession>
    <name>A0ABQ5SLY7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="2">
        <location>
            <begin position="2"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="104" mass="11627" checksum="A3B4A74DA8614461" modified="2025-10-08" version="1" fragment="single">ADVNDPAIKSAADYVTLTANKNNCNGLCASLKRHGNLRLLEIVSARTQVVAGTLYKMELLLEDEKGQQVLFTCSVWSRPWKKGQNGGDDESNHITKFHYQYINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLZ9</accession>
    <name>A0ABQ5SLZ9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="596" mass="60558" checksum="3E6E1BDF2381EA21" modified="2025-10-08" version="1" fragment="single">RDHRRFPAAPFVGTEPGPGPGAGADSLASPFGTSASSSAALAVVITPRPKPQAQLFANRGDPTGSEVVAQGLQVVPAGATARRDSGVALYRLSSPAGRKSVISETTLPFAFPAAVATSPGATAGGEARNTPVSPVELTAVAIPPTRLQQDLTACSTAAQRLRTLYRNCGFSSSEMSTDSDEERDDVVGGRREKGAKGGAAENGKEVCSPPAAAAAAAAYSMSPRAPGLPPKGAAATAGAIGAEAVLTLGHARGMGLMVRPQVPPPPSVGDVLEVALYGTGAPAANATQLAERIQSWWLRGSEEADADDNDNGDGDGDGGGGGGGDDRRTPAAAAAAPSSKLLSWPGWSAVKMLCAGVEDEIVQKMTEGKNDWGAAVNVAAPSSSNPTVSSVDGGGGGGGCHVLWESPAFGGPLCAGRELTVAALLPEKPCKPVVPSPLVARVLAALEQERIAGRFLRLPAAVVWGDGCPISAGEGAIAAEDDLWMVAPPPPQLRALEAAAFAPPQPSQPRGETRAHSIPSSFGDGGSAAPQDIAASSSAVHPETSVAMAYFQHEVKVFNELYEEESRGLAQRRATVVPNVTFRPLDTEELVTTIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMJ4</accession>
    <name>A0ABQ5SMJ4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_015557</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI70624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="116" mass="11942" checksum="DF83E42B016502D1" modified="2025-10-08" version="1" fragment="single">VAHCDSPQVLLGNVVELMCIASGLLSPAVIPSSPLLRVASAATALASEGLSLSAAVDRLSHVDPQNLDLEAVVLQFLQVLDLASSGAGLLESGQEVAVVAQELMAAKQRVKQESNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMN4</accession>
    <name>A0ABQ5SMN4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glucosamine inositolphosphorylceramide transferase 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_015809</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012444">
        <property type="entry name" value="DUF1647"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056442">
        <property type="entry name" value="GINT1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31389">
        <property type="entry name" value="LD39211P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31389:SF4">
        <property type="entry name" value="LD39211P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07801">
        <property type="entry name" value="DUF1647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24793">
        <property type="entry name" value="GINT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="595"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="959"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1002"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1042"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosamine inositolphosphorylceramide transferase 1 N-terminal" evidence="3">
        <location>
            <begin position="63"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI70775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1064" mass="115054" checksum="A12B9EEAB4B5D740" modified="2025-10-08" version="1">MAGSLSRSHRGGPTVSLYWLAYVLGSVLILAVLILDPYFNKPSGWIALELFTKKPVRHGFDPCSWDYEGSWKIGSFSGPSPTALNISEAPLITCADVSSTSVSFVRTPFLHIPGSGEQLDPSKWTIFYEMRNLQTFNSEIGVSESRDGGATWQHVGIALADPQLPLSSPFVVYDAPTKLYVMLPDSSMHGSGRGGGGFRAFVTTAQEFPLGWRPLEPSWGHRDPVRMRRILAGEATGPSHGGQAVNTAWRRLGADPDRVTHGSSSDDWDSDDVNADDSWGPPDHNIRFNGTTAVFVDGRWWIFTSIIQPSMLPWRSPRATPFIYTAKGSLLSKWFPHPAAGRQPSVAGCTSAPGAATAAAAGGMEGPLFSAGRPFVWQGSIHRWVRGCKRHRTDELTLLRATVANATHFEEEVAATYTPGDDRSWHSQRIGFVDVQPRPGTVGQWWGALSGDRYADGMHHFVVHELWFVEIKASLRDLMAAQLAVVLVVVALHSAAGGQRADSSSGSRGRHAAGTSGGDGGSDAAADSGCCRHDATLRQCVVQLLSRHPAVLSRVIGAAELAARWSHAGVARIGAVWEVLEIGARRAAAQPRVQFFAHASGACAMGLVVAAAVMLAVPSAVPCPRWMVVVRPFTSPPHYLPQQPFVDPAGPFDVSNLTIVSGCTMSFMDRLENMVGSLQYWEPHTPIVLYDLGFTSEQLAQIRCWRGVQVVRFPYEDYPPHVREISNYAFKPLVFDLAVKRYPVMLWLDCGLEVRAPLDPMRAVLARDGHVSAQQSGAPVNYLYTEMYSVPFFKLSEQEYDRIKNLPFCAGGLQGFVRGAASERLILGPAVACAMEINCTSPPGHSRSNHFYDQTSLTLYIRLNNYSSCLPREQFCTSAVKKLSWDPTASSAPIVMASRRHRWPKPYQHRVATAPAGCSPDPASNPWRSISSEHPETVTKSSSLLFKISFVYGQAAGDFIVQASCCLGFHLVAQGVLVAALLAGELRRRLLLPRGPKGVQSIFLSSTAAGGGGGGGGVGGSGGASQGAGDWGWGWGYRPSQLIGGLVLLVLLLVVSMTTSCRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMR8</accession>
    <name>A0ABQ5SMR8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidylprolyl isomerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_016025</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1050">
        <property type="entry name" value="Trigger factor ribosome-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3120.10">
        <property type="entry name" value="Trigger factor, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008880">
        <property type="entry name" value="Trigger_fac_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037041">
        <property type="entry name" value="Trigger_fac_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008881">
        <property type="entry name" value="Trigger_fac_ribosome-bd_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036611">
        <property type="entry name" value="Trigger_fac_ribosome-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05698">
        <property type="entry name" value="Trigger_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05697">
        <property type="entry name" value="Trigger_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="Triger factor/SurA peptide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102735">
        <property type="entry name" value="Trigger factor ribosome-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="Trigger factor ribosome-binding bacterial" evidence="4">
        <location>
            <begin position="95"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Trigger factor C-terminal" evidence="5">
        <location>
            <begin position="378"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="543"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="546"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI70931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="573" mass="63582" checksum="22635A0DD3E7B095" modified="2025-10-08" version="1">MMSVRQKPLSGGQSRSVAFTIPAPALGIRSFSLAQASTQLVHARLPHVLLASCSIPFGPPFAPCATPAPAKRRSSRGLHVCAAAVREKPLEFDVSVSVEELSLCRRKASITVPERAVKDVFKRGVKRLERDVIGNLRGWQPGKPLPLTMVISKVGGQEKFKTYCLEELLLEALPKGIDAARQGRPVDPASVEVPHADFPSMLERYDPATTFTFSAHFDVAPPVVWATPLEQLAVTIKDTGDFQTDAAAADDLIRQYRKQHGFSRVVAGRGLQMGDTLVMDMQIRAEASGQALPGLTHSRISFDTEQDVLGITPGMLGMKVGESRTFKMKMPDDYEVEFWQNMPVTAMVKVHEIFEWTLPEFDDSYVAQHHGGKWRSAAEMREALIASTAMQRLQQLDKQLEDAVVKAVADALEMAEVPSQMVEQLGERQFQAQLLQMIEDRIGSREDVEKLATEEMAAEFIAQRRKDLEDQVKFNLAVDEIFSSRGLELDEGAVEAEFDLRVRQMQAVGQPFEREELLEDVRETVKSVTVIEWLKDNIKREVLPYNNDNDNKNNSDGSAAAGTGQSEREPVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNW4</accession>
    <name>A0ABQ5SNW4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Terpene cyclase/mutase family member</fullName>
            <ecNumber evidence="4">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_017006</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the terpene cyclase/mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000117">
        <property type="protein sequence ID" value="GLI71700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02892">
        <property type="entry name" value="SQCY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032696">
        <property type="entry name" value="SQ_cyclase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032697">
        <property type="entry name" value="SQ_cyclase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002365">
        <property type="entry name" value="Terpene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01787">
        <property type="entry name" value="squalene_cyclas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764:SF20">
        <property type="entry name" value="LANOSTEROL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="TERPENE CYCLASE/MUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13243">
        <property type="entry name" value="SQHop_cyclase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13249">
        <property type="entry name" value="SQHop_cyclase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01016">
        <property type="entry name" value="Prenyltransferase_Like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01074">
        <property type="entry name" value="TERPENE_SYNTHASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Squalene cyclase N-terminal" evidence="6">
        <location>
            <begin position="107"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene cyclase C-terminal" evidence="5">
        <location>
            <begin position="413"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI71700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="761" mass="86236" checksum="261F845105FF694D" modified="2025-10-08" version="1">MWKFVSAGNSGGNPLLRSLNNNKGRQTWEFDATAGTPELRAQADRLRENFSTNKDTQHHSSDELLRLQCGDRIRAKKHAPPASEIPERLTPERVEAHLKGAISFYECLQQEDGHWPGDYGGPMFLLPGLIIALYTTGALDQVFTPQHKVEAVRYLANHQNADGGFGLHIEGGSTMFGTGLNYVMARLLGMGPDEDLAKRAREWMHARGGATYITSWGKFWLAVLGVYSWDGMNPLTPEMWLLPYSKWTGIGYLHPGRFWCHCRMVYLPMSYVYGKRGTCKETPLTAAIRQELYPMPYNNIDWNAARNQCAKEDLYYPHPLVQDVMWWVLYKAENILQGSFLRRMALAQCMKHIHYEDENTRYIDIGPVNKVINMLCCWLEDPNGTPFKKHLVRAADYLWVAEDGLKMQGYNGSQLWDTSFAVQAIADTDLLDVSAACLYKAHQYVEQSQVVEEAAPPLSRYYRHVSLGAWPFSTRDHGWPISDCSSEGLKAALRLATLPADKVGDPIPAARLYDCVNVILSYQNSDGGMATYENTRSFHWLEILNPAETFGDIIVDYSYVECTSACITALSAFLKQYPDHRRGEITSALRRAEAFIRSIQRPDGSWYGSWGVCFTYGCWFGVTGLTALGHNYHNDSAVRRCCEFLAVRQREDGGWGESYLSCQDKVYSQLDGDSHVVNTAWAMLALLAAGYHKVDPAPLHLGARFLLRMQQSSGDWPQQHISGVFNRNCMITYANYRNIFPIWALGQYRRLILLGEDEIRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNX3</accession>
    <name>A0ABQ5SNX3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_017015</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF74">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="195"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="311"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="316"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="330"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="396"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI71717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="431" mass="44334" checksum="7072E9242C6765FA" modified="2025-10-08" version="1">MAVSMHRDSMRLESLNNTLLHKDATVETATTDCKPSLLDLPEPILHTVIILSGSGAAAAASSCRMLRRSFRNALSCPELAARFLLKLYGRDTALFRVYGASGLREYLLMQGQATSTAVEAIVTELLNLGAALKPQERFLLTAAAGAGDTLVVRTLLRAGVNASSCGGRALVAASAAGHVAVMEVLLRAGVSARAENGMPLRAACREGRVAAARLLLRRGADPRAAASSALVEAARGGHLTLVLELLASGADPRVDGSRALVEAAAAGHGCVVLALLVAGAQPHARNGLALRQAAAAGHEQVVRLLQDAVHVDPPTETGSGCTPGNGKSRTTAATSSTTGGLLTSVSTSAPPTTSWRLASPAAGQPATAAAYYQHQQHQNREDLTSRNVVSVAKHTPVPPPPPVPPPPPPPPPVPSPPPPPPPPPSRPRPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SPW8</accession>
    <name>A0ABQ5SPW8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000119">
        <property type="protein sequence ID" value="GLI71709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="199"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="534" mass="57540" checksum="8A20C90294495CB9" modified="2025-10-08" version="1" fragment="single">MQHNSNPTKVTRTQRFVPAAHVTCPQPAGPFWTPETTGDRAASRSWPQSTEVVPANLLANKMSFTNVKTWVIRAREDNCSRDKVTTNNHMSVSSGGGNFGLDLGATPLRDGFRQVVDDTKEKLMTVRKVTAQAFSKEGWFRSWNFLKLQAQDKEQVHLPASAQLLQPSSALANRKECSTATAVVLVDCAQESADEHGEVQQREAAAAELRATVMELEKRHEAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKMREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRRETAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVGEEEKRREAAEAAAAELRAAVMEGEKRREAAEAIIVQLRAMVVKMEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQ06</accession>
    <name>A0ABQ5SQ06_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016668</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="307" mass="34186" checksum="273042C8093859A4" modified="2025-10-08" version="1">MSLSREYVKELSSTNTRSWFHKMESVLDFDGYGDYISPTFVMPAVGDANYVKEKEMVRKARAAITFRLDPATNASEIASTTGALDLWERLKAWAKNIEGAVVISASHAWAHLAKQGDESMTDFLTRAEDLFRKLDLDTNYKITEASAIMHIVGKLPPAYSHFDSMFVAFGYPKSFADLRIVLVAEEAKRMTDVKSEVKESTPVLFAGRERKCYTCGEVGHITHFCPKKNRSSVGASTSTSESSSGLHSAKQRSGVKCTWCGRPGHVEEKCWKKQDGVACCTRVQSPFTFMKAAMHSDIMKHNTIILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQX8</accession>
    <name>A0ABQ5SQX8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_017127</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000120">
        <property type="protein sequence ID" value="GLI71782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048624">
        <property type="entry name" value="CSN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF2">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21151">
        <property type="entry name" value="CSN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="7">
        <location>
            <begin position="210"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI71782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="462" mass="50971" checksum="C55CC41A2BDA7DE4" modified="2025-10-08" version="1">MATSMEEDTHVADHGSFDLEAYISNYTGHAKIDRLLFIAERSAGKPLELEALRMAHDELKKTENTQRYKDVVARIEGRLGAGYTLDRVWMENTDRRSASKGERLESELQSFKAAMMKESIRMGHSDLGDWAYDRGDLQTAFKCYARTRDYCTTSRHVVAMCLAVIRTALELENFVHVANYIGKAESTPDVVNDPITHSKLKAASGLQLLRAKKFKQAARKLCEVSPEMGSSYSDVMALHDVAVYGSLCALASLDRSELRTKVISSIGFREILELAPEMREMVHSFYNSQYAAALRCLDALKPSLLLDMHLSDSVPQLYALIRHRALCQYVQPFSSVNLAAMAVAFNTAVGELEKQLAGLIMDGAISARIDSATKVLYASKADLRATTFAKVLAAGEAYCRDTRALLLRANLVQHDFMQSGPLGGRSKKDRLERSLYDMGGLSGGGLMFGMGGMGLGGRSMMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RL58</accession>
    <name>A0ABQ5RL58_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase Ty1/copia-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013103">
        <property type="entry name" value="RVT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF483">
        <property type="entry name" value="PEPTIDE SYNTHASE GLIP-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07727">
        <property type="entry name" value="RVT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase Ty1/copia-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="341" mass="37262" checksum="7C86715F6F2293B8" modified="2025-10-08" version="1" fragment="single">ELESMGFKPSLADPGLFVKIENGEPVYVLVYVDDLLLACKSMAVIGQVKAQLLRAFAMRDLGETRFYLGFEIERDRTARTISVTQRRYISNILEKFGMKGANSRSTPMDANLHLTAEGDPLDTSVHPYSEVVGSLLYLAVCSRPDISFAVGSLARFMAKPTINHWNAAKGVLRYLKGTQGLGIKFGGGAHVQGYCDSDLAGAEGRRSTTGFVYLLNGGAIVWSSKLQPTVATSTAEAEYMATAASIKDALWLRHLLHDLGVSVGVVPMKCDSTACIAMLESPMSTARTKHLDICHHFARERIERGEIRMVHCSTAEQLADMFTKSLPKGKFKFCVAGMGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM64</accession>
    <name>A0ABQ5RM64_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000306</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36761">
        <property type="entry name" value="ORF03 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36761:SF2">
        <property type="entry name" value="ORF03 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="287" mass="32403" checksum="EC27B9838CF8A687" modified="2025-10-08" version="1">MLRGRYLCSATRVSHRPLVVCRAEPQRGPNSGLFTEISKYANPGILDQAARRYQLGLEQGFEEDELLALELGVSSIASLTEAQKVYKNKIKEKLAKRASELKAEEEALKERLARNLELGKRAYACGEYPASVRLLEQAVMDVGADTVLGGEAQLWLGLAYQACGREKDAITLYKDIEENHPSRKVKKQAADLRYILEAPRLEISEDERVKIPLIQSESWRQKQRTSYTPKYNRPPSAIKKNETYWDRVSLDAPDPLAMLPDRWYVRVAFAVVLLGATIYVNYAATGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMC8</accession>
    <name>A0ABQ5RMC8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="142" mass="15676" checksum="99D9DCC0F6354077" modified="2026-01-28" version="1">MGKTRGMGAGRKLRVHRREERWADKDYNKSHLGSEWKKPFAGASHAKGIVLEKIGIEAKQPNSAIRKCARVQLIKNGKKIAAFVPMDGCLNFIEENDEVLIAGFGRRGHAVGDIPGVRFKVVKVSGVSLLALFKGKKEKPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMJ3</accession>
    <name>A0ABQ5RMJ3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000534</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1384" mass="149448" checksum="72365FDE521ACDEF" modified="2025-10-08" version="1">MIGFKHHTMLERQLSSAGCSTSYISTPQLKSGQRISCKGHGLRCRCAGQQRPYRASQSPERETIRRSGQPHRPLGSVAASAATTSPSLPRRGDPDLSTNIPDAIPSLEQLEDLRSRAMKSSHLRAICLIQDDQSGHAAVTSNLARPTNTNRSGSVRGRFNETAARMASLLDPEDVDGSSDPAHASGNGSADIVLPNLVPVRSALSRAVPPPQSLSRPQPSRGGPGAGQERKFASGPRRQPSRSSAAASTVYRTRALKNERNWRQLPALLEELMRSGSLDAFMASTFLTHLAQLVAKSPPASQSDVETIECMQTTLLGSLELLMHEYRARQISNMTWALARLGLPSGAAFVRERMLPASYPLLPWYEPQHLSNTLWALATIGVAPDDEWLEEYLTVAFVALQKRALAPQHLANVLWALERMGIMPDQEWMAAYYDCVQAAVPAANPQDCSNILSCLALMRMHHHQPVATAALASRLAQRLQVLLSPDAIAASSANAARAAAPLTGAVTATDAAVLTPTPAHSAGAGLPEPAGAAVGVSDQAIANSLWALAIMGIQPAEELWDAIVAALRQRLADPTRLAPGMHPVALTIVLWASSTLGRVLPAAVTDLVYGRVRLAQMEVMEPRSLATMVWALATAPGEQARPPPDWVADAVAMSGRSLAQLGAQAVSNMLYGICQLGARPPPTWMSAVVNHFHRNLGLEASGQSVSNVLWCLAKMGYRLNDEGMDILFGLVRSQLLTWQQRCLEQQPQAGSPTPPPPFNSQEVSNLLYAIASMGYEIESDGELARLLLNAARVRLCEANAQELSNTMWCLAVLQIRPPEPWLEDYFAAATVRLCTFKPFDFAQTLYGAAKLRLPLHSLPAPADSTQAHSIGKVAAAATSSSAAPVAAAAAGNWLSAALIAAPQVIMFSNPQDLCNLSWALVQLRVPVQSDSSLVVAFTSRLVTLAGSCDPPQLALGVWALGKWRARPSKTQMERLEMATFRQVPSMRPHELAAMLSGFAGMGHVAPAEWWSDAVQQVAARSREFGPQEWTMVVYALARVQNDNSLTEMVLRLLPRLDRLPLPSLILISYSCGCMGPSPMGRQLVNRCLALLAAGAAAAPAKGNTTGAAADGARRNVAPVATKRDGSGRGFGSTARPSAVQSQLHRASRRGEDAEAGVAARPVCVASSTPTELSALLWSAVRVGIKVLPRDFLDQFYSHTEPLLENLPASLVASYLWAAGRLRFWLPTRWRKLLLERAEVQLSEMRARELAMCMAGLLWLRMWPTSSLLRKLRTCHEAQRNQMPEQVRNHADGALAVLTKRLTLARARERKYGYSRTTRLRWLLRVWGGNRRAAAGGATGFRGTRVVAAAPSGRISRRQALRQGILRKASQRSKGAADTVRTGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMP9</accession>
    <name>A0ABQ5RMP9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-Casp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000612</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027074">
        <property type="entry name" value="Integrator_9su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46094">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46094:SF1">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="39"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="855" mass="89372" checksum="591DC6F5FEE75490" modified="2025-10-08" version="1">MKLVCIGAGPCRCLVLRFRKANILLDCATPLYSALSAVGAGGSAEGPSSKSGTSRNGNAAAVPSTVDAPVTTGDTGRELPRTGGYNSKDRVEPKAGIPARSMEFFSNECQPSGIRRPSTQGGSSDSSDAPVYDLLDMTPFIPLLLTMEIHAALISTPEALLALPHLLQYSEGSPPPCNDSNKVDFRHAMADVGHATVQSCDATAGSGMGSFPRAAWHHMGPIRGPVYCTQAALDAAEQLVAERLIAEEVLAASLESAAPGTSHPTSLLPCCADMGWGAVTEAQEALLATCWRRRAPRHAVRHCLDRVRPVRYGQVVSIDSYELTAVPYSSGSGFGHAVWQLADGGERCRTILYLPDASPAHPFAPPLPLQLVRAPDALVLGPDCLSPCSGEPAPLSRPPHQLVKEAVLGAVVAGGSALIPVHATGEAWELLESLAGALASADLDNVPLLYIGPRSGTSLALASVSLEALAPERQAAVYIPQHPFAFDALMQAGRLVAATSLSTPAVQRCLQQGGPAVVLVAADSLLYPGGPGLELLLRFGPDPRSLLVLTHAAPPAVLRGIQRLYDKASAAVVAAGPAAATMAEASPDAEVSTAQSPMRMRVVHLPLRGGGGGGGGNGPSPASILELLLRLQPRHLLATHRDLSLLQQQQQQQQQQQQQQRQLHPLQPQMPVMAPSGSAAQQALGPLARESVVPYGWLHQVRVPLPRNVCGAVVSPDLLQRLQWLDGGPGLQLARLNCLLVFRNGAWHADPVPGGSTRVDSVAVGMALRAGGVMAADQLLLATAGGPALGPLLEALAGHGITRVSVDVEGDRIRVVLDGMDGELLLRPDGAHLISSCPVLRLIMTDCLMKQLTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMU1</accession>
    <name>A0ABQ5RMU1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_000665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI58890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="157" mass="15717" checksum="23BAF265461E337E" modified="2025-10-08" version="1">MTVHNGNSTSSPPMCPSPRATAHPEEPAVMLGQAGIPSACIGPPGEAVDKSTRNGCCNMVPHVTGSSCDEQLLVIDSNPIGEAWKASLKPTAGALSGSLEPSPMAQRSGTGSAGRWNMDPATTVVNAEDSYASGSSAAVLGPCGAPCAALPCHKHPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN47</accession>
    <name>A0ABQ5RN47_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Golgi apparatus protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_000814</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001893">
        <property type="entry name" value="Cys-rich_GLG1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017873">
        <property type="entry name" value="Cys-rich_GLG1_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039728">
        <property type="entry name" value="GLG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11884:SF1">
        <property type="entry name" value="GOLGI APPARATUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11884">
        <property type="entry name" value="SELECTIN LIGAND RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00839">
        <property type="entry name" value="Cys_rich_FGFR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51289">
        <property type="entry name" value="GLG1_C_RICH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045713835" description="Golgi apparatus protein 1" evidence="10">
        <location>
            <begin position="22"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1036"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="116"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="172"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI58992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1080" mass="118099" checksum="6A95B99FCCD7A71A" modified="2025-10-08" version="1">MKAQLLLGLVLVGCIVLPAFADEGSTVAVGQGTAAATNATGAATSAVPVNTSIAAAAKPAATASTAASTSTATQAETPKKDPNSNAAIAKAAVAAAKAAAAAAEAVNAAVEAAEAADKKSAEKEATTEKAEATGTKDVVAAKAEEQTPVAKPAANTTAEKAEQKPAAATETAKPREAEPANTKATKEDPVEVEPAKEEPATGEAGKEVAKAEPAKPTDVKTAVDAKERPAREKAPAYENKVVDFAPAANITFGDDINDEGKCAKEIKNYCDDVEVGDSKLADCISEQIAASEVPDSSGDVPEISDDCREDVYAFKIQRNSNINANVLLAKACKADADKFCNVTWFFGYKSGQVIACLRDVKAQVSKSCKQQLFKVMLEAAIDIRADPMLYEACKEDSESLCKGVKNGGGRIQACLRDKRMQLSWACEEQLFRQEMENADDIRLSVRLFSKCLPDKRKFCKDIEPGNARTKDCLEENREQLSSACKEEVDAMIERRVRDFRLDSRLRNVCENEIFNMCAYFGDLDDMDTYDSSVINCLQDYSAEIKNSQCKAQVKKYLQLASQDIRFDVPLAEACYEDRQKFCADVPPGSARVIRCLSTNRDRLSPVCRATLFDEEVRFSENIDFQYPMKAACVKEIERFCKDIPHGSARVIRCLQDNKGQKDFGKACNDEVSTYEAEISKDYRFNFRLHKACQKEVDKLCPGLCQANDGSPCGGKVLRCLTDKIEDIGDEACKKEVYYYEKMEVSNFMNDILLAEACRTDVELYCSNVEAGEGRVHKCLRDNRKKLTEACRREELLLEEKEANSIELNMSLLKACKAERQLFCSAVQPGQARVFRCLAENMNDADFGSGCKYQVVYKLQRRQANWKLDPPLRKACKADVLTYCANEDAANSEEGLVYKCMIKNYDSLSSGCQKEMGRAVHMAFFVWQPGAIITQECDDDINKLCLADRPNMASRPGAVGTCLATVLERMQASTNGGDLSPACSRLADIAEPPNMKAAFESSLAFALLKDQLEALEVNTGLPALTRDRRGNAQGVSLTGWMAMLGMTALIVLIIYGAYTGYRRLRGGSDRDYTLVVKQQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RN72</accession>
    <name>A0ABQ5RN72_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000830</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF2">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="7">
        <location>
            <begin position="1341"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="999"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1243"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="999"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1010"/>
            <end position="1022"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1573" mass="166778" checksum="4A54622C0B27FB17" modified="2025-10-08" version="1">MDPPSQEGDFMDEDMELQLALALSLQETTASDLGNAAAAPGTPPASAPAAAAPPAVPQAPAVTVPQLPNMSNLAALLSQGLQAAGAQMPLAAPPPPPAVPGPPPYQQQQQQQQPAAGGDLLASLLQQFLAGHALPTHQQQPQAQHYQQQQQQHQQQQAQNPTSALAAAMAAALGGVAPLSQPSAMAPVSVVASVGASRAAAARNGPPLPSGPLPGWASKLQGWYAAGKVSAAFSAASVVLAGPEQVQALVGQLTAALQVKVVADSAAIATGTLFLKTSAPAGGGREVSADSLRGLLTSALVQRKVAPKDRLQALHRAHDALPALAALPAGGAGGVGAAMELFSGVVCREAIALLRDDGADLYFDTGAQTTAFVDALAGGSATMCEPFLDSMCDASSSTDREAGVWRRLMRDGLRAAFAPAAKLADAAGFERPLVVLDRITSTQRLQQCLAALLLSDVEAAVRARSGQAAQALEQSCVLGPLLGASPMPKPQDALHFYKVTSPARQAFLETRGYPKNQSAGQTCRTVLQAMMARVQDGAGHLMLRLSRVKDGGLSREALLSWVAAVGRVNVVRRAFGETQEMRNQQDVADFLAGGSDGFLINVTGGCLRLAQPFVNGWLDLYRNGADPLAVVAAGAAAAPPALPRFADLFEKHLRPDYYRTQRHRLGDLSGVYNATGSRGSGGFTADDDPPSTAPPLLAPGDAVGGEGAPSFMADVFFLTQLMMHVGPIPSVYRRRAILHRFRQRYQMEMGLAAGADGDGEGGLDPADVRGGRDPWANPLATEMRLYDDCVESHLNEPLFADNLTAFAVLELDWMAWLSRGGAGDPAGVMRLVPEYVLGDALDWLTAVLYAGRADLVAAKPIAVIMRAVVTLLNANDVVRSAMLQNKIINLLLAMLASQLQNVQAREARGLALAPDRMSTGERALVGAVLGAPVTQRDLIPALMRAHVNAELVVGLDVDKDSYDKYGMRYHIDKILEELIKDSVLKRCLTDLAAAAATANGTTEPQQPGNGAAAAASSSSGSGSSGPFVEPGLFSDYASGIVNTVMHYFKDGLDRLADIYAIERSKADAAAWAALPAEERQRKEDFYRGQQRAAVGFLSMGVTNLKWLNTLTADPLIAAAFLHEPLLGKTAFLVVSSLELLLGDACKQLQVKKPEQYGFDLPVLVGAVLALQLQLGRHDRFVQAVVSEPDYSDEIMSRALRHLETHHQAHLETRLRTFLARTGELRSTPMPPATTAIATAAATAAVEAASPGGDTQPPLKRRKSGEGAGLEPLASEQQQRPTGLLCEAVQVPGREHSDAEVDKLYAADLGPMSIGEYDSTVPGGYFKEMARLADQDSGASRKKMRQLAKEFNDMQPGGRMALPCLAAAAIFLRQDSVRIDKMRAVITGPEGTPYEGGLFVFDIFCPAGYPNDPPVMMVYNTGGGKARYNPNLYADGKVCLSLLGTYNSGHTSEKWNPALSSIYQVLLSIQSQILVTDPMTNEPLSETMAGTAEGSAKTAEYNSRLQLLVMRHAMADMLMHPPPGTQDIVFRHFRLLRHRIATAVRRWVREAPSEDLRGKLDEQAARLLELLGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNR7</accession>
    <name>A0ABQ5RNR7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongation factor Tu, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_001026</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04165">
        <property type="entry name" value="GTPBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03694">
        <property type="entry name" value="GTPBP_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03708">
        <property type="entry name" value="GTPBP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035531">
        <property type="entry name" value="GTPBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="212"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="661"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="704" mass="74384" checksum="F4DF55CAA216C6DD" modified="2025-10-08" version="1">MSHHGKHSKGMHHLSVHNHDAHGWVEGITSLKAGDRAVELLAEEPERLPGAMNGKASAAAAAVAVAAAAGQGASEADGDSAASVDKAGPADGAPVATSSLAGAGPAPGVVSLTRPEDFEAVLQHALEVMAAEIEAVGEMVLDVGAPLGWGPVGLTAQQLEICLSRIRALVNPLQVDATVVQQRTVQLQTQTVPVDCITADVLLRRRPTSRGPLEVRVAVIGNVDSGKSTMVGVLTRSILDDGRGLARSKVFKHHHEETTGRTSSIGQHTLCLDSKGGILNDNLFRTQTCSEYIARASKVITLVDLAGHEKYFRTTAYGLTGHLPDYACLIVGANMGVVGMCKEHLGVALALKVPVFFVVTKVDIAPEHILKQTVQNLASILKKPGVKKKPFLVRCQEDVLLCARNMNTDSLAPIFLTSAVTGKGLDLVRMFYNLLPQRHRWVDKQRELAEFVIDETFGVPGVGTVVAGTVKRGVITPNTVLLLGPDIGDGSFKSAAIKSIHFKRLPVTTVVAGQTAALALKKTKRNQVRKGMVLVDERLKPAASWEFDADIAILTHSTTIQPRYQAVIHCEIIRQAARVVAMDRERLRSGDRACVRFRFIQRPEYVTPGTRFVFREGRTKGIGIVVGTEHQPADRTSAVAADGAGEGADVAERPDELEAGHEHTLADRGSAAPPSGPAGGAAVPSAAGAAGDAASATAIVAVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNX3</accession>
    <name>A0ABQ5RNX3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase class V domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000685</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF21">
        <property type="entry name" value="PYRIDOXAL PHOSPHATE (PLP)-DEPENDENT ASPARTATE AMINOTRANSFERASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="28"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="544" mass="58402" checksum="75250C07EA4CCBED" modified="2025-10-08" version="1">MGPSASDYARARAAFDYFLVGEDTCVAFMENAGGSQVPRCVVDAISNYFVYNYAQLGAGYSRSERATSIVGDAHSWLEVFMNTQGQGQVVLGSSTSQLAFNLASAFADCLGPGDSIVLQEASHESVLGPWVRLANRNGFKLHWWRADRRSGASHLADLEALLGPATRLVVVTHVSNLMGEILDLTSVVKLVRRRAPGAQVMADGVAFAPHRAINVFSWDVDWYLWSAYKVYGPHVGVMYGKRQAYDSLLSLGGQGPNFYFVPASDLTYKFELGGVSHEGCAGLLALQDYLLHLLGTSPTSPETPPQHHHQQELEQNKDQNQDQDPPQQLQPGCLGQSDHRPQETEGGIISIFPPLAPPLGAASAGKIRMSRSQVEAAFTVMTTMEKPLQEQLLSYLASHPAVRLVGSANADPAVRVPTISFVHRCRSSQRVARELQAAGFAVRYGHMYARRLVEALSAEGGLIAEEAAQRQAAEGEDPVAAAAATVAGAACADGGDTVASVATGSSAVFDEERAINEGVVRISLLHYNSPKEVSQLIEALGNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RP43</accession>
    <name>A0ABQ5RP43_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016833">
        <property type="entry name" value="Put_Na-Bile_cotransptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640:SF5">
        <property type="entry name" value="SODIUM_BILE ACID COTRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18640">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 10 MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13593">
        <property type="entry name" value="SBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="393"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="471" mass="50760" checksum="27F649962B9E18FC" modified="2025-10-08" version="1">MGEASGLQTAAAPVAEAPPAEKSRLKAYLKLFWDLICEHWFILGLCFAIGFAAAVPDFGRKGGWIAAQWTIKYGAVIIIFLLSGLSLKTRALLTVMARLHVHLFIQFICLVLTPAIGYGFARAMMESSTMNRDLSNGLVVAMTMPTTISTNVVFTKQSGGNEPVALVNAVIGNIIGIFVSPAWLYLYLGKSGQAPYANVIKQLAITIIAPLIVGQIVQYIFPNQVKKAQEKINFGKIGNLMIILLVWNTFCDTFHRKIHLEAQSWVPMLFLEIGLFLLFSMMCLALATFVPFKHFFRLDKSDAVAIIMCGSTKTLALGMPLITVLYGKHASVGVISLPLIIYHASQCLLGSLYIKQLKKWVDGPLPGAWPASLWRRVDPEDTGPGKVIRNESSKISNSSGSPSNSPVPLRSTEPLAPVAVDTPQATGSLVRRDQSGADVERGESAALVNGGDSASGTSVSRGSVGLKRAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPP2</accession>
    <name>A0ABQ5RPP2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein arginine N-methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_000842</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF68">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE PRMT10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="5">
        <location>
            <begin position="185"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI59017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="376" mass="42371" checksum="F248759B6C09162F" modified="2025-10-08" version="1">MAAKAIGKVSELDGEAPESTDFANYFCTYAYIYHQKDMLEDHKRTGAYYQAVLSNKRQLQGKVVLDVGTGSGILAIFAAKAGAKKVYAVEATNMAKHAQNLVDHNKMSHVIEVIQGTIESIQLPEKVDIIISEWMGYFLLRESMLDSVLVARDRFLLPGGALYPSHAAIYMAPIRSNLANQRTGEFQGSMEGWADFLQEMQHYYQVNLECLSDAYRTEQKDYYLHTSQWTDTHPQQMLGPAAPFIKYDLHNVTLEELKAPLRSEFVLHMTDGGPVDALCGFFDVWFRGSVENPVDNEIRLSTGPDPTGATHWGQQTFPLHPPIDCAPGDRLRVLLEVSRRLDNQRLLHVKAAITVEGNSIYAEQSKTPRTFRWNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPS4</accession>
    <name>A0ABQ5RPS4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucose-6-phosphate 1-epimerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122">
        <property type="entry name" value="APOSPORY-ASSOCIATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122:SF13">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="621"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="73333" checksum="66D7C0F1BF49CDE9" modified="2025-10-08" version="1" fragment="single">GSYISLFNFQLLKFVCVPEPVISEMASRILRSYVECSCSGRSSICSSAVSRNNFSRRPVLPQTVAPVSRLVSTPQRPLSDPSLQPPLWRKVAQRPQQHRNVAQEIGETSGEFSEDYVNVDELNEQWEIPGAIEFGTGQGDLPTVLLTHPQTGMQLVIYLYGATIAQWLKSDGTATFYDGPDELYVPGLPLRTGVSLHFPQHREGLLPEHGFADRMMWEVVGAGFDCPEMLEQLEEYLEMLQAHGVVSEEMLLAAKEGGEAAEAVRAALTNEPPPSHMVDSDLSPCITLRLRSTEDTLSLWPHRFELLYKITLMTQDVTEEDIRDIRISQGMDPEEHSDDDEDGDEDEGEDDAGEGEDEDVLVVSGRTLTEDAAGGNDESADGKSQASSAATRRRGRPRKQDAAAGGLGPDTNEEDDGAEEEEEDAEEGYDEDDMGEAEPDTVRQEPYQPAVQIKQEFWVRNLDEPGSPPMRFNLASLARFVTLQQPLCSDWVKVLGLGGSQVFDYTSDPRYPALSLNEEDYLHYRGQPIDITYIGAAEATVYLCPGNRTHFEFIQRNGFRDMFVQQPALADEPDFDRVATLGTGYIASTKKLEAGGEWFAESIIRFHERYWRPPIFGDDSVPPLPPISQELLAEGDADEGEGGDAYDGLPGDPLDTAPDQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQB0</accession>
    <name>A0ABQ5RQB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_001723</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825:SF2">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE 3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="534"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI59762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="562" mass="58463" checksum="348E0772CFE17593" modified="2025-10-08" version="1">MAPSSCVDSDASAVTGPTTAVADSATGAGTTSPAADSLVDWSRVRLGVDAAPTMFIANWSPDTCRWDGGELVPYGPLTLLPAAQVLNYGQSIFEGLKAYRYDGCSAAPAEHRVVLFRPDANAARFEAGARRMCMPPVPPAMFLAAVQAVARANAAWVSQAVFRELERFGKDDCVPPAARGSLYIRPLLLGTGPLLGLCPAPSYTFLVYAVPVGGRAKEGRLVPLDYLIHESRHRAAPRGVGSTKAAGNYSPCLLAQAEARAQSCHDCIYLDAKSDTYLEEGSGCNVFVTHGSVVTTPPAAGSILPGITRASLLQLAVVLGYHVREAPISWKEALQADEMFGSGTALVVQPISSVTFKGRRVAFYRTSTANGTTAGAGKLAAVNGHANGNGQMNGYANGNGCMNDANSDSSEVEEEAEAEEPRKVDAGAAAAAAAAAAIDDDCESAGLSYRFGGKAAAELQPEERAALAAFAPVPEPASLPPGVGPIAHRLYALLTGIQYGRAPDPFGWTVEVDMGGPTAGPLHLDQLLVSDEVEGVSEHPPVPGKGLRPLQARPVFADPNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQB2</accession>
    <name>A0ABQ5RQB2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_001690</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006053">
        <property type="entry name" value="PRK08201.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006579">
        <property type="entry name" value="PRK09104.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF12">
        <property type="entry name" value="SUCCINYL-DIAMINOPIMELATE DESUCCINYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="4">
        <location>
            <begin position="257"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI59740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="573" mass="62161" checksum="0F4529057D3C55EC" modified="2025-10-08" version="1" fragment="single">VWTYTECFYAIVNLPDQRFTCDPQSRQTRAMRNLLPQSFGLLLLIAAARFSYANLTASDTLRAVLHRIESQSDRYNKDLAELVRFPSVSALPQHEGDVLAAAGWLAERLRAAGLQNVEILATEGPQPVVYGEWLGATDPHAPTVLVYGHYDVQPVDPLDAWESPPFEVSIRGGYYYGRGVDDDKGGLLQAVQAVEAWLQETGAVPINVKFLLEGQEEVMSPHLTTFLERHAGRLAADVALSADGGQPAAERGGISLGLRGVVSLQVDVVTAGTDMHSGMKGGSSANANHVLAVLLAGMRDSRTQAVAVEGFYDDVAAITDQDRRDIEMYGFNPEKEAAELGLLGYVGEEGYGILEQRWLRPTLEVVGMAGGFTGEGIKTVIPRSAMAKISCRLVPRQTPQDILMKVARHIETHCPVYARCNVTALGGGAFPFSNPRNSPGNAAAAVVHATLSDKPPLYLRDGATIPALAAFQQVLNVDTTKFGWGLSERIHAPNERLRVDMYDKGRRAWAMLLEELPRHFAAAAETEAMEARGAMEATESPHGDGYWHGALGTEAAEMGRDIPDRDGALHEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRD0</accession>
    <name>A0ABQ5RRD0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_001492</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1061"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1107"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1143"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1172"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="616"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="753"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="859"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="912"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="623"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="924"/>
            <end position="941"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI59572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1255" mass="130186" checksum="21D416FC8534AC1E" modified="2025-10-08" version="1">MLRATQARAARRVGVCEQIISVSLFVVGLISYQQFAAPYCAGLPHTLPAASQALAGYPCASSGFQRTIYVLRKTPYVLYDIVRLKFADRWATIAILALAVRMVAAFTMPSHLYGQLAAPLTMYVERAIMPTFHIFLGPKHHPSTTALKCMFNTYPVFDVAFFTSLLGVWDVSIAVEVTHCLLAALAHTAVCYWHRDAAPGGPANLSWVLFRRFLAMLTTLYLAAVHSTSQGRRGRASHLTSGRPATDSTAVATGSHAFGAPRADSAAEKADGELRKPTRKEVEEKQQRDELQQQGAAGGGVEGTAASCPGSISANACLEAAAGQQHLGPSSTVEGAARRGRAQAGGEVDEGAVLVPAPTAPTGGAAEAAGATPPGGIVKLKSYRSPIRRTMVRVKIPWAEPEQLTPGFPQRLADLAAQRGLVLRGAYVRSGCVEVVLVLEALLHPALPPPSPSPHGIPKNCRQPNIRISTSTSIPFDGGEDLEIPAIIRALGLEPPTPTQHQQHSREEGRTGGHDGSGVTAQSRRLSAGGGAEHSLGDDGSGGSESSLCCAVLVRSLLPRVLPLSLPSPMLPGPDASATHGSVYETVSAPPPSTCTCTTETAPVAALLQICVSSPPHTHPHLHPQQQQRQQPLPGWRGTLGAAPAEEEPQSIEVVVRSCGRYLPARVAKCSWVVDKARGGAPSQSLVDADGYDAVPDRLDVGEGCGVTGDPDGSGGVVGCPEGQCDVELLAAPPAPGLVLIEVRRLRRGADNSAPLRPAAPGFPGITPHGASDVTTPLSGPSPLHPDDIDLVPVLATDDAAIAEELNGLVSSWPSGTNRELDELVYDLGTWMAAAAAAGTLCTGDAVAATPPATAAATATRTSAAGSDPWEGKEVQGGEEAGGDAAMLAVLGPHLLQFAESAGLAATAASIRKRMGPPPPPQQQPRGIQEPQQQQQQQPQPDVDEEHQSTNKTSGPAAKMAGEGDECSARCLRTGFGGRSEVRGGRWGRADGGLQLARLVSALLQVLGLHTIAPSSPAAYDTFAEAWCVAQGHTVQLVESLALITMLLRAGRSGLQPMGPTTIVCVMGCGIGALTTLARLVLPYRSWVRLARAVQIPRYLGHTGAKVLVALGFSAPLGMVTYTTGAGALLLEGLVLPGASLISFRWALIVAAIKLPANVAMMLVLGATRTPLIAVLLAARIEALALTTTIACNVYMRLACERHITAAAAAAATVGAGGGAGGAAPLTSARRSSNSPGDLEEYVATLSSSGSKKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RRU0</accession>
    <name>A0ABQ5RRU0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_002417</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF169">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1868"/>
            <end position="1892"/>
        </location>
    </feature>
    <feature type="domain" description="SBP-type" evidence="6">
        <location>
            <begin position="65"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="164"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="270"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="821"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1100"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1317"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1442"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="191"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="444"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="832"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1337"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1350"/>
            <end position="1366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1897" mass="193201" checksum="77A3840C5C1354DA" modified="2025-10-08" version="1">MSTDEEPRGPSKAAWDSTAYIMDTYLGLARGQGADARKTGEESRHAARHAEATLLPKKSRKATRPAICQVDGCGRDLSGEKAYLQRYSVCDMHFKSDVAMVQGQEMRFCQQCNKFQDLREFEGARRSCAARSKDRNLRRRLHTTLQKGGASEELQVPAAAVLTQPPPQQQPQSGSPGRSGCKVGRDDAAGSDDSGGQAGSGHASMGGSAAMARLPPEGGSGGSEYSLDAMGTASLAVSAGARRGGHPVSACGLQSRNPAMTLLMNPQQRLHQPAGSAGNGCGASSGNPTAVQEPPGNRPGAWLSLEQMRQSYDVDAGLSPNLAVALTRRGAPIEDIGAGDIGGTASGGGSGPTLMDLDGFLSASCSNPCGPHGGRMQQAVGPLASALALEGGVGTAAMREQDSVYARKMSAVGGAANLAGGVGGWAGPAALSLLDVYDSLRPQQQQQDLHQATPQLQHPPHHSTAAVQRCLQVAGGTPGGSGMGSWGSGPMHRLQSFGAEGGGGGIGGQQQLTLGTSSDDGGGVMLLGQLHGTVTGVGGRGLAASRDDGALAGLLGPGALRAGGGGSSLAGLRSDQLEGQRGAVASSGRVLLQQQQQQSGAMPALLSVPSNPRHDDLTAMIKERRAALIRQQLLQQQHRQQQQQPLTGQHESLVKQDQISMLGQSYSGEGPYLSLSASDQLQLRMLSAGGGSASEAPTSGAAAAAAGGGGLVDDYYDIIPVLGSRAGGAGGGLSASESLGGGRNLVLGAASAGGSVPLAAAETLVIGRRQASADLPLDISLDTVQLLEQYALAGGANGTLGRHSSGVNGVHLGVGSGVGSRGLQQQHSMANGAGGSSGDGGGGFNRYDSSDAGAVGAGGGGGGFAAHGTGNIVEGGGSSRLLSVLFPGAGGREDALAARVCLKLFSCTPDELPTDMLIRLRRWAMAAESDVMQIFMRPGCVHLIINMRLSQGGQATLERTVLGGGDLDAAVKALRGAFESCGLLRGRRVVVQAAEGPRGAYEYNGAAGGQARWVSDTDLARAPSVLAVQPAALPCGVSSEVALIGRNLRRPGTRIYARFGSWSYVLHPTPRQTGVSAAASQRDDVWAAETAEQQLCSPRTLPGEEAARGSWTDGFSTPSASSSLLGGGYGAGEDAAPQLYDQSDGFVSSCPSSSSACAARSAQDSASGLLLAEAVAASLAAPDVGQGRHLQPMQAATETGVEPQSPLRNHDHGSCLEVVYVTVPALPKQGLVAVEAVSTDVDVMGAWAPGVVCQNAAAAAELNTWLRNNADVISGQWLLKELGLLLDYDAVLQSLLQDTSGPALAARPQHGEELAAAPDAGSMAGPGPAIPGRGSHQRTLQQKHAASNTQQQRQQQQRQQQQQLQQPVMPVISNPLPRTGADRNGMAVAAAAAGNGALSAVPYSASEASDLPPIVSARPDGSLELRLLPSDTSADLTRTRMAEGPGAAGHAGGPSTARDPQRGLRHGDVQSIVLTSPHAAIGSSADGGCRVGPRAAPNRMMLSDDTKVPDAPGRMLEPSLRRLLTHPLLHQACRRALLTAGSRLLAFAVASGLSLLANGLVEWLMVLEAPTLGLVAGGAPFGAVATTSNGAGVRAEPFTGSMLADTFGRVVDVAAVGTVRGAVVSASRAFGSGSRASSGGASATGFALCETSCDNDSSHDSSIGSSSSGMFRNLSMGLSLLHLAVISCSRVMLMQVLDWAEEYGRPWALEEPAWPGRVTALHLAAAAGALRGNGGEAARLLLSLGPEAQNAWLESRDALGRTPADIAHAVGLSELNYFCLRGEEPPAPQTSDVPRSVAVEGGLAPKSKGGRVLVDRSAATGMIDRKARTASGGRSLEAAPDLWTVEAIGQARVQASSHGSAAFLGMPLLLGALLLLLLAGALQVVGASIGAAGMRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSK3</accession>
    <name>A0ABQ5RSK3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002707</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="50"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="379" mass="40220" checksum="20340ADDC9FA0E73" modified="2025-10-08" version="1">MMQDKASAMAESKFAGCEPELRVDLAAYGDRVTPGRANQITLARVNTPTRNILRKFDNNGDGRIDATEIQTIVTSLVAEKFKSKAFKIGLIILAVFTAVLLGAMFGLTWAVVAALKDTKVQGGILVTNDKEQAPVLVANLDLTISGTKLVSRDSSEVVATSTIRTPGLLTTGTAFDKLVTLQNLVLVTPSKSSFSLAILGIARVPTNSSGGYVIKVLTAAGTLILENNEWSAPFNMTGTLLEDVFTRPAAQQGRRLADDAIRPQVYVICEGGECGETLDCGQDNTGPCSMSAIGTSFRTPVDHTGLTDANTGTYSCSLLTGLVNYCSAGLKDCYGATPTATQTSRYLSQYGCTSNCGTWTYNSFYGECEGSPAKKFCCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSZ4</accession>
    <name>A0ABQ5RSZ4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_002889</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="45"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="210"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="429"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="520"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="582"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="680"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1151"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1223"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="520"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="601"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1223"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1253"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1287"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1302"/>
            <end position="1314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI60687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1360" mass="142315" checksum="75BC5C5B22EFB0E5" modified="2025-10-08" version="1">MSILRDLVDAVCCCLPGEEKLEQIGIPRSLKRLSDWRAHPTLREYDLEKHLADGGFSEIWLVRSRLSGAEQCLKVVNLCKPGLLPEDVDILRGEARFLRLLDHPSLLCCRDVFETKYHMVMILEYLSGGQMLDHLHQVEHYSEAQAARLFAQVASAVAYMHNLNIVHRDIKPENVMFVRPLEECVSKGRSPRVKLIDLGMAAVLVPPETPEEAAAATRARKQQRRHGGPAAAPPPPRREAPRGCMGSPGFIAPEVIHGSVHTLSMDVYALGVLLFAMLTGRKPWSLKDSRNLSYAERSIIEAPGLKDPIFLSLSSAARELVLAMMADDPRVRPSSAEVLRHPFMTQSLSAGGGTAALDDVIKRRMAQLASLRRFRGLSFAMMASRQEGQDLADFTKQLTERRKAIHRDLLVRAKTRHSMDMERSTLQLAAGHQLRGNSGSVDRKKRPQQPPLLPQAPQLLRKRGAVPVAGNNPASPSEDRGSIDSACSQQPLLMRQQQQQEQEAVRDTQQSVALTNENAFNHCKQNQPQSTHLQVDGSRHSLDSSMASSVSSRQPLVGGGGGGVVAVPSHHTSHQAIWLAVPLGPPPNQGHLGAAETAGSGHRKSGSLQHPVRRSNSHHQQRHVLDTVAEPEEGPSGVEGAAAPLLHSQQQQPAAGASGAVRSRGWSITGRQNAAAPIAASTGTLPPLPPPQQQQLQSTSRSRRFMKDSELELAAVDPLALIRELPIHAMGSALGRAHSAPENLATSLLPLIGRGDVGGLRVASGGSHGATDALLDRVAMGADMRAVSANSWTLNFLENADLLAALTGVPLAHRISSASLDTSVHGGTATYGEALAAMISAARRNSGDAATTANAVLQRLRSDVSRGGGALSPEASAHQVMVAALAAARAGSSTHGGSATTMSTGQHSLPTWTVFATAATAPGVVPAETPTRSVAAGVNDRPAVDAAAAFNKPSLPPSKAAIQGDVTFYPSSGLHGKRTGQPRVLFGDCASTPPALPPPLDWADTSAVSPSSVSDIPIGVGLSACSGSALPLLRTLRHGDDPDGAGGGPSCDVKYTCLHLSTSSRQLGVALPMAAASPAAAAAEATVKATAPAAAAVMVSAAFHPALPFVRRPSLALVDVEDRIAAKRASLGERFHAGQLWIQLHGGGGSGSPAAESAAMTGASGDLSGHGRSSRLDSAFRGSAASQHSPADGALKSTPPNASPKWNYGLGLALQQELRALAQSASASPSGSALKSPPSLARITECSDLGDSPGPECGGDPAAAAAAVSARAGKPSRGQQPRKRLPTQPTSETALSTRASKGAPPAGKAGEAPDAAPPAPRTHDQAAGCENRWRSPFEALAVEPNVDAGSAKKPIPGADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTI9</accession>
    <name>A0ABQ5RTI9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_002711</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF72">
        <property type="entry name" value="NEUDESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0754">Steroid-binding</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="8">
        <location>
            <begin position="39"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="8">
        <location>
            <begin position="140"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI60553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="237" mass="26686" checksum="BC67F0A65402E735" modified="2025-10-08" version="1">MDFVTLALLFVIVIILALIIRGSRRSLPPPPDKYELGDVTLEALRFYNGYDWSKPPLVAIRGKVYDVSNKYDVYGPGKPKSAYTGREIARALALESINEKDFTADLTGLTAEQLERLEQSVQEFERLHDHVGQVVPLRKFTAEELARHDGTDNSLPLYLSIKGTVYDITKGKQFYGPDGVYPFAGKEVARAFALFSTELEDCNDNLEGLSYTELENLRDWIGKFNSKYPIVGRLVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RU32</accession>
    <name>A0ABQ5RU32_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="169" mass="16920" checksum="2E317B7C8A616389" modified="2025-10-08" version="1" fragment="single">LDKAGRGGPGRWPSAAAAAQSMRDYVGALLEERRAEAMARAEAEAEARAAAAAAAAAVATAGTTAATVLQFVDEPRPLTSPDPLRLGGHKGIAGMVFGSDTSRPCFGGAEDWEGDHVDDDSDATSSVRTGHCTGGSAADAGGRAAADAGWKAADAGWTQGGKEPTQRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVC2</accession>
    <name>A0ABQ5RVC2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BAP29/BAP31 transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003958</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040463">
        <property type="entry name" value="BAP29/BAP31_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05529">
        <property type="entry name" value="Bap31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="3">
        <location>
            <begin position="4"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="180" mass="20244" checksum="466786B7553A0A87" modified="2025-10-08" version="1">MSGWKLVVYWLLPPPLILTILLILPMPSVARKGLLKVTRQFMCWKIGGQLQLLHVALTITGIAFAVTATTTYSMSREHIDPTLTPNQRSYALARKWRQERNFWIAALTFLLWGLLGRFYQLMMDHMALRERVRYLELGVVAPVSSSAAAATRVKGAKEGVEPSAPPAPTEDLKREGKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW26</accession>
    <name>A0ABQ5RW26_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">starch synthase</fullName>
            <ecNumber evidence="4">2.4.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_004050</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18189"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.21" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03791">
        <property type="entry name" value="GT5_Glycogen_synthase_DULL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005085">
        <property type="entry name" value="CBM25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46083">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46083:SF5">
        <property type="entry name" value="STARCH SYNTHASE 3, CHLOROPLASTIC_AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16760">
        <property type="entry name" value="CBM53"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01066">
        <property type="entry name" value="CBM_25"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate binding module family 25" evidence="9">
        <location>
            <begin position="348"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding module family 25" evidence="9">
        <location>
            <begin position="531"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding module family 25" evidence="9">
        <location>
            <begin position="700"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="180"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="243"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1259" mass="138870" checksum="427A41799CEF73EE" modified="2025-10-08" version="1">MASSMGRMGLAGGVSTHRTVRKGGDGFSSRRGSCGARHSQVPGLKALRKEDLELLNQAESYMTTEEQAELRRLLEENHKLRQVVTSSAKQMSPQQLDHFTKEYSNLAKQAEQAGVAVDTAFKQAQATSPISSGAAARSAGGRLGAVATATVSAGGSGTFGYSGRSYGASVLVAEPPGLAPTAAPTAPASADTSNSVVNGASGSSSTRGFAVSSREYARDMASMSVELDATLEDEDQGHEHTQQHAGKSLDQQVPAASTRTEPYAAVASRFEALLSERRPGGVVNAAQAQLLDAREVANRKQDLASEEARKAALRAAAAAEVESHDPAALVAGREHWMIITVPEKPVAGAPMKLYFNRNQSDKLRHRPHIVLQYGFNQWELMPETGNRVDLSPASLPKNGTMDFWSCTLQVPEETFEVNFILHDNNGAYENNNGMDFTYGVEAGITYDVWIDTAAERAVAKEIARKEAEALAAAEAKRQAVELMAQQAKDFGRHKADDLKNSISRLQDGALTVLFREGTPVYRILPTTPVAGGKVKIQYNRLSGPFGKFPIPEGQTVTLLYGFNGWQNSGKANMTRVPPPSASSATDAEDWFETEVALPREAAAMNFVLKYYEHYDNNGSQDYKIKVGLPPGLTLDAWADQVAEDLRTREYERRLAGQRVMEEKTRRAEEKKKAAQDFVKAVERRKVRHVLFTSPEVVTAGSEVTVFYNPRDTPLNGRQQIYLMGGWNRWTHKRHFGPIAMHPPVEGGEHWQATLSVPKDAYKMDFVFADVPGGDGTYDNRGGFDYHLPVEGSPIREPPLYVCHISVEMAPIAKVGGLGDVVTALGRAVKEQGHTVEVVLPRYDFFLHSPVVKDQMRYETEFDWGGTRIYVTTAVVENVRVFFIEPRNGFFATPTVYGRYDDEVRFDFFCKAALEFLLKTGRQPDILHCHDWSTAHVAQSYWADYHPYGLYKPRVVFTIHNLNYGQKKIGEAAHACQKFTTVSPTYAFEVGGQPVIGPHAHKFMGIRNGIDPELWSPEDNQFLPMCYDAGTVVEGKKAARQALRQRLGLNGWNDKFIVAVVSRLTGQKGVPLIKHAAYRALDRGGQFVLLGSAPDPRVQADFDALASQMGGQDAAFCFKYDEPLSHLIYAAADMVVVPSMFEPCGLTQMIAMRYGAVPVVRHTGGLRDTVFDVDFDKERAAWELYGSSDWKRDGIDATNGFAFSGTDGGALDYALNRAIDAWYNDRAWFHSLQKRVMEQDWSWNRPAIDYIELYFSALKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW65</accession>
    <name>A0ABQ5RW65_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004369</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="2"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="312" mass="34210" checksum="FFEE70CF9E5089E1" modified="2025-10-08" version="1" fragment="single">MCVVCWKEFDYRHNPPVDLGCGHFGCGSCRAAIKNCPVCHMAIIATSDSKREKPRMMQGTKSWPIPRRRQRLGEELLGDLLDDDHVTPTLLGSADTASSPRVRMLERAPSSAAGSVTGRAPQGVAISARHVARGSLVAGRAMLSCAVVPTVRIGFFVGRTLVSTTCGAVVSTGRVLAHAACSFVRFAAAPRRSVANFDGNRESAPNIDDPTEAQSRLHDGKHCRRRQRRQLQHQRNGAVPEPISNIVRGRITLRNSRNTRIRIEYCRDCIVIANGLDSVELLLVCCQRVKLVLRNCSGLTLRELQCTDMGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW68</accession>
    <name>A0ABQ5RW68_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_003838</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF17">
        <property type="entry name" value="FIDGETIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="463"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="127"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="196"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI61508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="712" mass="75492" checksum="706920F0B7FF18B3" modified="2025-10-08" version="1" fragment="single">MDALLQLRHLHFNAEGCSVGSEQQQAKALQLKCFLSSVGAREFDPSHEAADALLDTSASARRERLWDAVMYAASLDKEFCILEGSLANLDLPGAVLKGCPLLDLGEWMAAAREASAQTHQALLDFHQRHHQEQQQQQQEVATVQRNSIDGMQPPGPRPGPGPAPGSPSGCSAAAAAAAGQPGLSARAQQTFGAVHCGGGVVQSRPGPGFRPPVPPTLQQPQDASAAGIQNPQVPQNDGGQRWSGGSGSSGNGPSRWAMGKRPALIDVGAPVDEDCSSGPGVSGGGGGPQPGGFRTARAQLVADSVRKGQAPPTFSNQVPRTGLTRPQKKQQTGGGGASGASNIRGFVPPFVARGLEGPPAAGGGGKGEPADGDVDSPYPPRLLEMLAERNLLSYGPEGNVVVPEPLAKLDPKIIDNVFNEVLDRSASIGWSDIAGQDAAKRLIQEMVVWPMLNPQLFRGARAPPRGLLLFGPPGTGKTLIGKAVAANISATFFSISASSLTSKWIGEGEKMVRALFALAGCLQPSVIFIDEIDSLLSARKAEGEHEASRRLKTEMLVQMDGCDPGSCERRVLVIGATNRPEELDEAARRRMPKQLYIPLPCTAARYQMLVNTFRQGSEVTTCLSESDLTKIVERTAGYSGSDMKNLIQEACQGPVRDLFRSLGNVTNVTPSDLRPVHLKDFQMACKAQKRTVSDAEVERYEKYDSQFGAKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWI5</accession>
    <name>A0ABQ5RWI5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_004411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI61895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="183" mass="18701" checksum="4FB840D282FBE0A0" modified="2025-10-08" version="1" fragment="single">QTCWKALTGTYNALVVAATENARMREIVPPPSAAASGKNGSAEPVVSIIPVSSQGSFMYLRPTYKLASCDNVTGSSQLTVRIVVQDCDTFAAITTPLSPADCTEDNRCTRNASACSSPCSTGLMATLEGSFIVDASGIVSGLSLRVVPDAAVAFLLGAGGAATTNTGSIQGTAESLQGDLKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWZ0</accession>
    <name>A0ABQ5RWZ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="29587" checksum="A734C331447B6618" modified="2025-10-08" version="1" fragment="single">TFNIIDTDGFRFLTWSPFGTGFKCPTYNNAYNIPYTDKLALADVTPPPPPRPPAPAPPPSPPPRPPPPPSPSPPPPPRPPFAPSEPSAPSLAPAILLFVAEWTDATPDAQGMVIEDDNDFVDVDLTVSWLVGKTVYSIDVETYDDAETFSPSTSSARPPPPPIQTVIRGGVHGDDNTITGFNNESVFWAGRYGNDRPDDSIYNICLTLYGDALNKTGLVLNVTISVYNEGILVKQYRRTWDTSTSKGLPPGDVCNSTSGGFMGNYDFNPRFDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXB0</accession>
    <name>A0ABQ5RXB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="41573" checksum="45BFABC2586ADD96" modified="2025-10-08" version="1">MTLTYAEALQLLGFPPDSQGSLPPVDVIRRAFRRQALLWHPDKHACSNTFSSHATSAPHSHCVTNDLEADRPIPPLQNTRPLHGRVHDSGPGTADTVSVLATALPECLLDPTYRTQAQVEEHFKQLASAYQVLLQAAASSPDVTADGDSYFKAGGSGSAVPGKSEEEKLLDELSAELVLLEAYKLRLSDFDMVFLHQHASGASTAVWHVWPEAARSLDDVWRRIRRRVERRRKKREGAGGGHGRDDSGFSSRAELAVRAKWDLLSEFLADSLAASWKPQRRAESHLSGRSPVDGAIGLELNGRSPGNGDSEGYPQQQQQQDSAATSGCVLASASAYAAAPISAAASAMPYRQRGRLPAGFYWRWLKRLVAARLLFFVFLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RXW3</accession>
    <name>A0ABQ5RXW3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052758">
        <property type="entry name" value="SRC_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44200">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44200:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="1558"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="687"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="736"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="881"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1371"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1704"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="741"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="893"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="919"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1381"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1707"/>
            <end position="1720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1737"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1772"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1823"/>
            <end position="1834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1834" mass="181504" checksum="75C5130BC5259E57" modified="2025-10-08" version="1">MKSSSEIHTSIAVPLQTGTSASFVLGLGTDGPSPSRKPAGRARSSSSSSKIPQRPTGGVTSSATHAASDEVYVPKTSASNPAPSVTEQPAGTEAISHQPFGLPQSTVPMNSGTAAAGPAAEGAFPVPRVGHGPPGAPGSSQLAVPIPIFTVGPSDGQTVGMRTPRGGRMRTGKAKTSAAASPLRTPPPPAPVAGSTALPFSTLGLDSLASMTPIDDHTYQKLAQNTSSWAVSSESPMDITPQGSTISSSSNVFVYSPLVGGPCGRPAVANGGAESAPGDGAGMASSASSAPMPFPGFASAVPVTAPVGAAAGGGGGSAAAASFAATGPVAPAAPAPVHTAPAAPPGIIGGTSTSGNLRFPIQIQIPGAREVQDMFTSVASASASGGPAAGGSGNSNRGGDAGSGAPSASTGLHPNGTTGQHASVGGPAGAASTRQQGQQHQSIRTAAAVLTAAAKKLPDEHTSTMAQGGPATKRGHLGVAKGGAVGNDCQPTKDNASKASRVTIDNGGATTAGGNRGPNTAVPFSTVGGSVAAAPKTAAASRASGVATAAAALGLAAAGAVAVASSPAGTTGSEPDILVAVAEQLEHMAQRTREAAAAAAAARGASGDGNAAAAVEAVIATLANESNPAIRPVLEIMLRPGAMDAKVAAAVAAARESAQEAAAAPGLAAAVACMLGALKLDDSQLGTQSQGFADDDGSTPRPAAAAGGGGGSGSRGSSAGGIPSVPFVFGCASSGGGSSQGGASAPPAPAAPSVSPAYQSTTASASAPVGAAPTVPSRAAAATTNQVPTQQPMSVPFMFGVPGSSCQNGTQSLADSKAAARVASSAPLAFGSTTGTAFGSAAGVATAAAAAFGTAAAGPSTAAAAGVPTHQAAPQQAFLFGASASGPGAPQPSARQAHQSGGPTHSGESANMSTGIGNAPDSASLMSTSSSPMSTTPPAAAQHSAQFPTAVLSPTSGWAGAASAAAMGAASGGEAGFTFTVGDASAPMANGGVTPGRIGGRVRPKITPRKTRATAPMDTSQPAGFAGLAAALPPAAAAPSSAAPMPSTAAAAAANGVSMAQPQPAAAAAAGHAERHAGAAAAKAATALPQASRLQSSAGAVETGLDAMAVEAVRLKRDADEKKQYGNQKYELSDFRSAERWYSEAIELLENKLQQLGLAREKVQQLFPNLKTEIAVLYSNRSGARLMTAKPQSALADALRALDVDPRFMRAASRAATCHCRLGDFAAAHRIIDAAMDRCSASSSHYQDMLKKLNEVVDLGSRARAATAAAVSAVSSGTREKLQEAADQLAVIRDMVGYCDVVAAARAVLALRLGFPREVLVLLAPHPEAAPQQRSAPWRLWLVLQAHYYKGDLQEAMTTCRELQLSLETVGPESGTAAEATGSGSQEQGDGGAAAAAAYTTSAHVTVPPAAVLTELVESFQQMLKLKEDGNNAIKQNRLPEAAESYTRALGLGCCPAYAAVLHANRAAAHAGQGAVADAVADCGRARALDPSYYKASSRLASYMLELRRPDEALGMLDPLVKLTQHSDGLGPRPDEMNTIKERLMEARSLAVWQKTPQHYKLLGLTSNCSEEDVRKAYRRLALKHHPDKALAAIKVSMALPGRAGGAPYSWPMLGTTELEARVREEASWLFSFINQAHEELSDKTRRRKVDQLLDAEQPSQSGRYGAGAASNPYGNGWYNSGSGRYSSFSNFYGVDPFFFHRPAGSGPTGGGGGAGGPGGSSRSRAATGGGAGYGTYGSYTGGSRPSSARQAGANGTNSNGPARNNTAGASRGNGNGAGGAAAGAAAGGGGYGGGYARAGGGGGPGARSANGGRKWWEGGNDFSDDDEEDTFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYD3</accession>
    <name>A0ABQ5RYD3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase M50 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">VaNZ11_005286</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M50B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39188">
        <property type="entry name" value="MEMBRANE-ASSOCIATED ZINC METALLOPROTEASE M50B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39188:SF3">
        <property type="entry name" value="STAGE IV SPORULATION PROTEIN FB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M50" evidence="13">
        <location>
            <begin position="59"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="GLI62622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="327" mass="34696" checksum="D47B750421D3C4E5" modified="2025-10-08" version="1">MQWNEFSKFPWSIPLGSVLKIPIRLHVTFFILWIAQTLGSIYYGTWYVILSLIVMGPLLLLTIVVHELGHCLAARSIGGTAESILLWPLGGLAYIGYNTTYSKAVWVAAAGPLTHIPMVAIWVALLFPAYHASTGSWHITLHQPPVDQSHFGHALIVWAILLNIGLFAFNILVPAYPLDGGRILAITLMAMGASMRMAAIATVAIATPVAVGLFVLGIVVFQMVTILVAVFILYSTFQLFMAIRHDKLSEHPLFASVSAGGAGPAAGVGGGAPAHTTRILPNWMYPGSQASKSGTAGGFGTPGYPGPSSGMVTGRQPGSAYYVGNAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYM1</accession>
    <name>A0ABQ5RYM1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="584"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="877"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1042"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1275"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1455"/>
            <end position="1511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1565"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1735"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1900"/>
            <end position="1945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1989"/>
            <end position="2046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2060"/>
            <end position="2119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2168"/>
            <end position="2203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2238"/>
            <end position="2265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2292"/>
            <end position="2342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2357"/>
            <end position="2458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2572"/>
            <end position="2597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="472"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="879"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="896"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="919"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1338"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1483"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1581"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1735"/>
            <end position="1754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1756"/>
            <end position="1771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1932"/>
            <end position="1945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1993"/>
            <end position="2029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2067"/>
            <end position="2085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2091"/>
            <end position="2119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2330"/>
            <end position="2342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2395"/>
            <end position="2406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2921" mass="303201" checksum="D85F0C1EB24A8DFB" modified="2025-10-08" version="1">MPNQSSITVPGFGEVPVVALGTIDGSFGPAGFGSDDEEPIFLSPIQRRLRERGGLFGFGGGTLSMLHSLAFSGLGAGLGLRPVSGSGTATGRNAPNSSLGGSNNNLGAGHDSNFGGSGDGGGNGSNGTSPQHRSLGLLDLHNRVSDLVRVRTPVRRSVASSSSGFTTAQFALPGPGQAVRPEVESDVDEDGHRVVGGNEDSATEDGEDDVCEDEDEALDYMDVDEAGEGEEYGEDDFDMDACDSHSYEAESVEASAARSLDSSGGRRRRATTESSRSCGTDNEASRCTLPALGRAGQGGDPLNGADGCSSNDVGLSEEDEPLLAAHRAAEAQAGEHRFLEGSAELQSSPETRHVTPPGLDAAAEIGRASGDTALPPLHPASGVEAVRAAGAIGAAQGLASGPRWRDNGTLSVAWAAARFALSAMLAPVRFVMFVARLSSGALSMPRASRVHVRPLLQVTVAGRPADRRLAPHSDGPAAAAGSGPCTVSGAEGQAGWRWQIMPSAHASHAQGDELSVGTCTNYRRPTLSSPGDRAQILADAEADNDAGGSVRYRAPTDVAATPTMAPPMIEPIGLLLPALAPSQGQIEHDAELPERSPRDQLTRRLENRSRHVPPVVIRARLSHGCDGGNAASGAPGAGAATPPLLRVHCGSAVSSADALELGEQASVSNPLQQLHPSQAATLSLLPIPLLFHGTLPTTPVPCEGIPEAQAEAAGVTVQATFVAVTDEETVGQGRDTAPACTAVASGTEATRTSLPGFESLGSSGGQGGNMTGRDDVVDSMAVGATASASPAWIIPPPSGDGDVRQPTLDLPLNQLMVAEGERSGPLGHPVASTRGSDVPLRADEWEHSHVAFAAVQRVSGCVAAPVTTAGAIAAEQRSRRAAGSRRQRVRQHSEYEAAGGRVAAAAEATGPRNLTDVGAEADEESSTSESDADIFRIWGRQLLDADLEQLFSLAAYRCLGPGPIPESLQVTREQGSGTASPATVSARPFAARILGAANRAAGETGEGSRPLAHCVAPLSSLPSRPRPFGTPRVLLGMTRSARGHDRPLRPRQPGGFSHGDHMPAAPRDSGAVLQGGTDVAVAVAAGAAARAPLLLWGNRLNNPEFSEQLLMRRAASMENVHCYVRTMPAPAATAASGRRGMVTQARQSMVFPAAPPFSAAILATPALRVLEATAFEPGLLRLPNTELGVTALNMLSGPTPRPTGERGLAVAGGMSNEGGGVPTLLASAAGAVAEAAASGSPGSGLWDGRYNEERRHRPARAIQDVTGDLPAIGATTLRPALPHDNEEDAAASNQAPMGGWRGAPSRLRQLGEALRLSEDRRRPPQRQQRTGRHAWQDHGTTSNVLDSNDNRDGSIGPWRYYYSDGNTNHRALLSQIPPRVRVPLLDAIFPRTGTPSSSGCNNMLGALHVGPGAQFTAPTRTSFMEVPYSVRHVDWVQRRSRSSLVDVGAGHAHNCSAAGASSENPQEHRPGQPNDFGEVGSRDGSTVSTSSEIPDLVSDPASSPEWDWPHAAHQRQRQRPFLWLQRRHELIAPVLFGWQRALVPPEAPDFPGLHMATRSRLMSRRARATYTSQEDVDADTQSETGLSSDFHTPGEGSPAVAAAVQIMVAEGLDNSAASLQTAADVGPSASGGGTLTDRHSSRLRCLEHEDERTRARLAWLCSDTDDGTSDGSDLPIYWWASPRDQQPSEGGVRFACGARNAVTGSLDHGGIEVNVGAIEMPLSSCSTEEGLEANGTAATADGDGSDGDLLPPALVDSEEENEETSAPDDETGSGLSSVPFLIDEDSFSTLTVNYGNAGSSSMEGDIVAAEGEGHSQALSDLWLADGYQQDYGDWGNTPSVRNRRHCTAGERVIGRYASSEMDSDNHIPDLVSSSELEPFLHSRADRGQAGGGTIAARVNPITAPSRLESNGHARSRPGSRTISIPSEHRQLLQPSTRRSPIGSTAIASRNRTYALSGVSPAGIRSESAGVSSVSVYRRMAEQMYLEAPNLFPSDQDGGAADSDASSDSMPPLVSLNSLHSGSRGSSGRLSLEDNDATEPRTGGPAVVRRRLAWAEYQGFPSSQRQQEASLPSAAAARGTATELAPQRSHRGSQPAQPRRTASISTSVAGTTDDLTSTEADSTWGAAVEALLRVLVDVRSGDRVSADAGGSVASRAVWSASTSIAHLPVTTAAPTNDPRPRPIAVGNGAGSTGANGSSSRGGPTSQIVASLVRIPADVLSNVPPGSVLSITIPAGGLSTSVRGGSDNISGAGSSGDGGGSRPRSNTTAARILPLPLGAFSSFPLSWRSYLPLEESSGRHPNDNPGLPGIYDERDIPPWAARFAPQVPSEPPTRSESAAGSGLNNAQQVVASAATLTTRTGIQQNPNAVAADDSNPQTRSIPASWEAQWDDISFSNADDEDGVDDGGDDGNTNYGDNNMVANQDGDERNCQSASDSEDQQNDTESLPNSLPDLVPPEESPAGIAASTAARLRGLEGPASQEVTAANTSEREVVLPLASIAAQRELTPRLYFVDMSADVGTPMRRVEGRDGEGNVTVDRESGGAAAQAVSLDDARAAAYHLHSAAAHLPHELQHLQDGSHDRSPAPADVPGAGEARETRGRRVAAPVLGDSIGGYSDVGVMEMLTELEGLPQPLPTELHGRLQHPDWRQPHLRDHNSVGPVLQGVAEQSHSGAGLTAAVAVNALHEGPDSRHVRRGSAPARVPLPPMPDGAGAAPPQHSPLAVVRGSNTVSRYAGPDMEEGDAEPQSLLLGTATVLETSPTAVSGSGRGVPVAQLALGPTREPGSVPTRRNAVTYHRNVLLNMLEALQISETAASSSHLGSSHGSIMAAAAAHAPSTHATAPATRMVGMGFGPQGRVTRSASHIAATAALLRDDSFVAQLLGGLPGVAIRPPACCVQETVRMLQSGEEGLGLGCKFELCNVIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZ45</accession>
    <name>A0ABQ5RZ45_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyl-accepting transducer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005104</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="735"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="1080"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="1300"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="424"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="88"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="483"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="563"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="626"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="688"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="729"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="751"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="794"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="810"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="831"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="877"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="905"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="922"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="964"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="985"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1033"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1074"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1096"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1139"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1155"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1176"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1222"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1248"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1264"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1282"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1296"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1308"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1322"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1350"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1386"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1396"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1436"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1459"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1486"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1507"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1560"/>
            <end position="1573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1580"/>
            <end position="1595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1652" mass="173541" checksum="6FF0DA5241FE6EF5" modified="2025-10-08" version="1">MEDSSRAWASASLPANRVASPRTANGNGYQPAVTEAWAEPPPTSSHFLPDIREHPSRQSQQRAPLYPLPPEVPSADAIRGEARIRGLKNELVTTKNRFEQELQAKESENQKLREYIEQQRELLSSQKVTIRELERNVAELTRTAAQQRATYDRYSVKAEAKVRALQGRLRELEQEHAAAMGGMAAQLERARKERTALALGLSAVAMQASGGESAHPEDTHMDSERAAAEGYEALPRTAAAWVSLRYEMDDDGNLASFSRAPGSVNRPFDRDGARAVAALVAQTRRLYEQRATYRAKLRSTGQHIEHMQAVLDNVEADFNLVNSMIDQMNSEAARLSADYGLPPLLPTDVPPPDAAARLSERVEGVRMLLQRMSEELLVSDAEKKALTAQVLSGQFLQDINSTTLEQPVDRQAIIEAYTEKLKQLYGTGRRPPVTNGTNGRRSPGHSRRSVPSPGPPMRSERSVVPPAVPPPPDRRPTHPLSPKNKTTSSRPSYQSHPSPPPAEPEERRTSEKASRANALSDHFSEVLHGGSKPAARARRAKAPADSTRASSDKEAPTKSPRGHATEERAAEEHARPQIVDLEDSSPPVPVKKATSGKVKAHREPEPEPKPQREPEPEPEPQREPEPEPEPEPEPEPEPEPEPEPEPEPEPEPEPEPEPESQEKEDQDGYDEAFEDEPAADKEPEADDAAANAEAAADAADDASIAASEAEEAAENASEAADDALKAAEAAEDADNAEEAEAAAEDAKEAAEEAADAAEEAAEAAEEAAEAAEEAAEEAEEAAEAAEEAAEELAEAQAALAKAQAALAAAKTEEEAAEAKEAATAAAEEVEEAAKEAVEAAEAAKEAAEAAVEAAEEAAEAAEDAKEAAEDAEAAEDAAERAEDAAEELREQEEEESAKPPVRLHLRPDSAQRDSVRMRVSMDPEEAVGDADNDEQAQLETVEEDAPVETEERAEEPVSDDDDAAGALAAAQAALKAARAAMDAAADNDEPVPTGDEDEAQETLEPEEKEDQDGYDEAFEDEPAADKEPEADDAAANAEAAADAADDASIAASEAEEAAENASEAADDALKAAEAAEDADNAEEAEAAAEDAKEAAEEAADAAEEAAEAAEEAAEAAEEAAEEAEEAAEAAEEAAEELAEAQAALAKAQAALAAAKTEEEAAEAKEAATAAAEEVEEAAKEAVEAAEAAKEAAEAAVEAAEEAAEAAEDAKEAAEDAEAAEDAAERAEDAAEELREQEEEESAKPPDSGAEEPAEQDVAEEEDAAGALEAIKAALGDVKELMDTAEENDEIEQPPVAGEESAPAVAEAAAEEAEDAADQAEAAGDKASEAGDKASEAGDQASEAADDAADAADAARAAAEAAKTAEGAEEAAEAAEAAQDAADDAVEAAAEAAEAAEAAEEAAEDAAEAAQEAAKAAEEAAEELTEAKAALAKAEAELADADTPEEFAEAKEAVEAAQEAVEDAAEAAAEAMEDAEEAVEEAQEAMEDAKEAKEEAQEAAEKAADAQEAAEEAQEAAEEAAEEEADEPKSPVRVHMRPASGKQDSVNVRLSMDEPAGGAAEADEPAQEPEEDGEPPVPTHVRQDSTQRDSVKDRDGPSAADDAAEEPAFEVPPAVDEDEEALQAEMRALEEAEMAVQMAAELAKDAANDDDMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZM2</accession>
    <name>A0ABQ5RZM2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005268">
        <property type="entry name" value="CHP00725"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041164">
        <property type="entry name" value="LDcluster4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052341">
        <property type="entry name" value="LOG_family_nucleotidases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00725">
        <property type="entry name" value="TIGR00725 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43393">
        <property type="entry name" value="CYTOKININ RIBOSIDE 5'-MONOPHOSPHATE PHOSPHORIBOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43393:SF3">
        <property type="entry name" value="LYSINE DECARBOXYLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18306">
        <property type="entry name" value="LDcluster4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102405">
        <property type="entry name" value="MCP/YpsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="17860" checksum="0CABA1E3A5BEE01C" modified="2025-10-08" version="1">MSIRKPIIGVMGPGKTELHTEPVEKHVELATELGKQIASHGWLLLTGGRNLGVMDAACRGAKSAGGTTIGILPGPDALNSSDAVDIPIITGMGSARNNVNALSSTVLIAVGMGPGTAAEVALALKARKPVVLLATQPEAEKFFTSLDASLVHVAADVEGAIVHVKTLLAAQSEAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZM3</accession>
    <name>A0ABQ5RZM3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01390">
        <property type="entry name" value="HMG-box_NHP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="83"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="83"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="149"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="181"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI63078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="207" mass="22280" checksum="2B02646652E51B09" modified="2025-10-08" version="1">MAGDKSAKETKKAAGGDAPKTKAKRKHEGKEETERPKKAPKAEKAAVVPKAKAEPAHKGKGKKAAEEGGKAKKEKKAKDPNAPKKNLTAFMYFSNAQRDKVKTDNPGVSFGEVGKLLGEKWKSLGANEKAEYEDKAKKDKERYAKEMEAYKEKGGEVATASTAKPKAKAPKAPPPKRVVDNDVDNEDSSDDDENGTDAGADEDPDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZQ5</accession>
    <name>A0ABQ5RZQ5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">VAMT003</name>
        <name evidence="3" type="ORF">VaNZ11_005684</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="61"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="369" mass="40610" checksum="CA4A627CB5C16715" modified="2025-10-08" version="1">MSGESVPSERPRLQLKPRDPNAVQAYEAQRVRAGSNPFGAAKPREQVIAERLGKTEEEVLKETVKKERPKLRLNSEQLEQRKQAEARIEEVKIQLQLETANGGDQSALEAELASREADLEVLLDTFEALALEAAQRGEIQRPSERRQQQQQGHGDIEAGRLGGRAGYSERGGYDRHGHGRGHYDKESGASRGGYGGETREYDRFRGGFDRDVTNYSRGAYGQRGGRGGAVYERAPRDGGYDRYERSYDRDGGRSGGDREGGRSGGGYERNEGDYNNGPRGGRGGDWGGERGGYSRGGGGYDRRGGGSSYDRRDDEGLQVGHSGGMDDGIRGGRSSRSGYGNRGRGYDPAYEELNSMDYVSPYQNTQDRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZY6</accession>
    <name>A0ABQ5RZY6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006097</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM RBM34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12394">
        <property type="entry name" value="RRM1_RBM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12395">
        <property type="entry name" value="RRM2_RBM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034221">
        <property type="entry name" value="RBM34_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF25">
        <property type="entry name" value="RNA-BINDING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="367"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="511"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="45"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="590"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="695"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="269"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="604"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="741" mass="75764" checksum="F8C641331E8A8FC6" modified="2025-10-08" version="1" fragment="single">MTSLFGNLFGGSGGTTDLFSNSNKFKAPEQHLLHIVGAEINQELYSETENPSKKRRKHKGNPTTAAGSAERTVDTPEANRRGSISKNAGAAEDKCRLKESKRGSRRVDEEGGGCDADDTDEGNGGGSAIAKTGKKKDKKRVASGLSADVELSGNAPVEDHRSRKKPKGSTSAASDAKEAAEAAKASANAAAAAAEASHAPQQSLDGSKCKLPREPVAVEADCKAVKKGRKSVVATAGGAAATDCGPQRWQDSGSGATKLKASKPRRTTEDEDEEDYEEEDGFIDTDGDSGSDSEDGGNSNDSDDGSPMDEGQEDEGEEDNIEKGNADSDISGEEGGAGHGDPSRAERDESAKRRRRSADEEAARLARTIFVGNLPSSFASAPKLLKRLFSGFGAVESARIRAVPVKMDAKMPRRAAILSGKIDVERGPCTAYIVFEQPESARAALGANMQEVEGHHIRVDFAVPQSAAAAAAVAAAAVHGSGKAKAKAAAAAAAAAAATASGSVGVYDPSRSVFVGNLHFQTTDEELIGLVLGQAATQPELANAVEAVRVVRDRVNNVGKGFAFILFKTKAAARAALSLDGQVLRKRPLRVTRASRQAPATGTAAGGGGRSSGGGKGGGHGRGRSSAVASDPTSWQGLKTKGKVKAVRGPKPVGLGGGSGSMGTRTAALGQAPVNRLRSAKRPAVAARKASAAAAVRGPKLDGASVEKKKSTIKGAGASGGNDGANGWCRGGDGWGFGGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZY8</accession>
    <name>A0ABQ5RZY8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kinetochore protein SPC25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_005952</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Chromosome</location>
            <location evidence="9">Centromere</location>
            <location evidence="9">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SPC25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23784">
        <property type="entry name" value="RWD_Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.50">
        <property type="entry name" value="Chromosome segregation protein Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045143">
        <property type="entry name" value="Spc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013255">
        <property type="entry name" value="Spc25_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14281:SF0">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14281">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08234">
        <property type="entry name" value="Spindle_Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5 9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromosome segregation protein Spc25 C-terminal" evidence="11">
        <location>
            <begin position="156"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI63182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="226" mass="26248" checksum="457BA7D25A701A25" modified="2025-10-08" version="1">MSIDLNDLEAELVRARRKASDWSALRTQFVLERKDQHAAFLCDQTAKLNSLQGHKAQLQVQAEQTNKRLQHEEEEVKRTEERLVGANTEASVSERRLEAAQAQLRKRQAEYRQKVEELENEQKDRQQQLDNLRRQAARFTERFGLHFRSQTPGDFTVVMTHIDPKDHKKEFSVAVRMAPNDVYTVTKCDPHVPDLDELVNEVNRTNDFAAFVKAVRSRFVAVAQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S020</accession>
    <name>A0ABQ5S020_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="30634" checksum="746D914D871D0DA2" modified="2025-10-08" version="1" fragment="single">MSEQLAVYRRCRRQYPGTAACVLLPAFCQRKAEASLRGMRRVHGFEPGTVLFNGVDREGMLAPWPGTSYSVDVWYEAPDEGAVGYGPALSALTLSAGQSLVDNRVPVVQIGLNGRTLKALVDTGASDDFISVTEVNALGLTPESSDCPQVTLADGGKHPIVGRITLSLSIGPLRVVTRPYVLQGLAETAPYIMGSSTLRQYRARIDMETATLHMCKGTLSIKVPFLSFEHVTPFEQRLHVLREVACRFPRVVLAGIPCPLNQVLVYCPLTSVLAHTTRAIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S096</accession>
    <name>A0ABQ5S096_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006186</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF62">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN 60E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="668"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="698"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="737"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="778"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="869"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="900"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1666"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1700"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1735"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1783"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1826"/>
            <end position="1843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1864"/>
            <end position="1887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3064"/>
            <end position="3087"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3141"/>
            <end position="3170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3262"/>
            <end position="3286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3345"/>
            <end position="3366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3378"/>
            <end position="3401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3413"/>
            <end position="3432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3469"/>
            <end position="3494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3517"/>
            <end position="3538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="3559"/>
            <end position="3583"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="666"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="1665"/>
            <end position="1895"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="3024"/>
            <end position="3293"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="3344"/>
            <end position="3593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="104"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="430"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="586"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1024"/>
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            <begin position="1151"/>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="5">
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    <feature type="region of interest" description="Disordered" evidence="5">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
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    <feature type="region of interest" description="Disordered" evidence="5">
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    <feature type="region of interest" description="Disordered" evidence="5">
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    <feature type="region of interest" description="Disordered" evidence="5">
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    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="3871"/>
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            <begin position="143"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
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            <begin position="218"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="GLI63280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="4012" mass="427919" checksum="A1C38EAAD556548C" modified="2025-10-08" version="1">MAHESPPRHARKGVVKMPQDATCYCPPPGAGRGWWPQRSATRWEARGGDTYPDPAARAGRASELFEALRAENPDVDDEDLLSGPGYQERQWRFLQERSQQEVMERLERRADTGAPTSLRAGGYRVSPGVSDMDGLVTTNGRASFEHHRSLDRHSFESIRRSHENQRGGRELQRRSQEHQKGSGELQRRSQEHVRGSRELQRRSQEHQERNSSELNRNSQERQRRSSELQRRSLDRRQRSNECVRDSRDGPNFYGTRGPSAVSGIEPSGNSWTVTHRRHRGTALEELLPPQPAPQPPSYSIHQRDDTVGSQPPLHDGSGTPAAQASAADDQSVLRARHKGADGPCSRSDTLWTAAAGQTGSSTGFGLSAAPARNDANSADNSTGVEVTQGSASQREPPFAHKSNYQPAPQSAITEDDLLAAANAFAAVQLASSPQNGPQAQSQPAATRNEDEEIRASEDGSRRWRQEPPRAASPVDDATLVATARHSPSSSTPWPRWDNNDIGPGIRIGGQDGPDSGFAGANRRGAVKGRGRGIGQPEILGGGPGPPIVARMMPEDALVSVGSGTGAGGFAAALAALGLVEVQPLSDHIQRDDDRLPRPPGPLPPGSRQTSRPQEEASIGIGGIGPRAMSDRTMTIETVATYEEFGDLSQYSLMRRWCIRLCANPDFEMAVTAIIFANCVTLCLFDPRQPDHYGLNRKLFWAELAFNICFSMEMVLRIVSMGSPWAYIRRPWNQFDCFMVVAGYTVFVPGNSSAVNAIRALRALRPLRTITRFESLRGVIVCFLEAIPLLASVGGLLFFFIFIYAVAAVQLFPKTYHWVCLDPYDVPLNIGDDPDMFGCGGSGRCPANYTCKLLHKQYTVNVAGFDNTGMAMLSVWQALTLTGWVFMMYRTVDAYSLVAVIYYVTLIFIGAYFVLNLFLAVLKIKFAKAQTLFHNQLALQRSKARRNSIVTLMNKVHSKWTEFSSKRSQASLLNSRLSSAISSRMSSVNSGPPSLRMSAAQFGDGDNSFQELDCDGRRLTAAAASPQLPRTYNHIQSSTSNLASERSPQLLGQRDANGPPDRIEIPAAADSLRNDDTGKSWGSATHDGVPLPPLALAPSPPKTASMTSDSDSVSLPTRALSIRARQSVLAAADGGDLDSSCPGSDAVVRMGIMDDRSGGGGSSQPSPMPSRTPARPALISTKQSFLKGPRPMRAVSFRHRDSDFVLSPRGASGVAEACEVGASIAVTAASTMNAPASSISVGNLHGAPETLLPSSFSSCDVLPSLLPRVRPPSPAPKGPRRWQMRSLRRVAPTSDSLVDGGARGASSAARAGSGWAVLEGVVSPCGVYASHSGWSLSRPAAATSAAVTADDYQPAARHRAESKQQSWRPQLNAQIQEKQQQQQWTHERLPAARRTTTTEITPLAPTGTTTAISPVWRTESKPGIYEPSMQSLAIGAAGPVAEAVRFAGGEEDGYSVDGLAAATGGSRAGTGSGLVTVADFASAVNTTEVANATAANATAVPAVAPTSMAPAPGMASSLGNSHAQAKSFHISSGRKEICSPPSSVGRPGGEPSPIPLRRLERKLTHGEDGRAPPVRRGTSSVLGGSSRSNGYGLIQGQTSTAAGAQDNNDIGGNERTYSAAPSSMGATNMAMNVMSTEEFDDFVSDHPYMQRQMLRLQYRTRIFVNHFVFNQVFMLLIFINTVLLAMEYNNQPASLTRFQNVANWVLTGLFTAEMIIKVFGLGFWDYVRDSFNIFDALVVTISLVEIVLSTVAGLNAARALRVLKALRVLRLFKLFRYMQSLRRIGEILLSAFTSFAAIATLLMLFWLVFSIIGMHVFGIKSLDGYEWPNFTTFLFSLISTWNVLNLENWVNTMHATIRVTNWGSSIFFVLWIVIGRYIFLTLFLAVTLEAFEAKYDANAVRMGARGGGFTSLLGSIWSGASSLRSGLSSKASSVMSSVRSSRSGQNSGSSGRSAKGGGGGPNEEANGDASGRFSAGSAAGRAGRGGQHYGPGSALPAGIGYLQDAQAAAAAEYGESNEQRVNMQKMYSIKQWIDTGSMEVLKAPEATPDASTVRRGGDVPLGRCNGISACPPGAESPMAPCGGGNWPSPMPPRGTGNSCAMPLGAGRGGRGVPYIDDSAAQSLASLEEVPVRAGRGGGSGSGGGGGDGGDGGRGVSEGFDLDSTCHTDLTLLQQQQSQQLQQPPTCRSNQTSNVAAEVGRPSTAGPSIGPTGRRGSVGPHLHSLTHAVRRDLEGVPHSADGTDSEVGDLITSVDGGGGNSWARPAGPSIGSSITGSGADSGELILSRSHGRSRLMRSTTSLDTSAVARVTLVPNYCFKQEPSGDDCELPSTAPELAGPGLESSPPSPAPEFETPAKISAASHGATLAVAGTATGRGVVVLDPPARAQTDATAAAASVFVSPASSRSRVRTRNDRDASGALPADATVSVGRARDTVEDSPCDGYQDMLDCFADSAEKEPAAGPDGGDVGEVHAAGPEASCPNKIELASLGQEVIMATFQDMLRPSHDSGVAAVAAAAVPKQQQSQAQSLGQLGGPEEGLAAVPGGSVPSPSLSGLQRNVSMTRQPRREAVWDEDGSKEAAAAAVSGDRPAGHRHPRSEQLQEERQLFATESCTLLALADELDNALASIEVRSGGAAAAGSGPGGDAAALQRLGSPTAGIASIDRSGCSPTCSELDSERTSPRGMPPPPSRLELLVAGSAQGAADGIGNAATSAVISTGGGGDDSSRTGSGSGFGSSIGPGTAGYVACQSLQDSAHSLSDVGIAGSRQRAALSSIGPATAALGSGAAAVPWRQLPDHSGVPLHPPPLSLPVLGDSAEQGCFSDATAASTGAERMRVLDTTAARDSLNVGGGHGDGELIDSTETSYQRSRGGGGGGGGGGGKLHSPNGAPILVEASSQYIGSEPNGRFGLDPWLPPPMKPSAGPAAGQGDEDRGRRGDKGPAMRRISEAGSIGASDMISRCDSFNSRASGASHTSGGDDSSGDDPRVRKQRFRKRRRASILIEGTGKAFWIWDEDHPAREWMYWVVTHKRFDYTMFALILLNCVSMALENPFIRPGSPMEKALFWSNVGFTIIFTIEAAIKMFAFTFSAYIKRITNQVDFLIVVTSLLEIILDTVTDSVDAVSALRALRALKPLRLLTRSAGMRLVFKSVTMSLMSMANVSIVCILFFLIFAILGVQLFSGRFYRCNDDSVAGISECVGTFNDPRTNNTIERTWKNDFPNFDNTGNALLCCFITATLNGYTEIMINAMSAPNEPGHQPRQFLNPGAFFWFFGFIVVCAFTLLNLYVGVIFSQFSKIRMLSETGSAFLTSDQNEWAELTKMVFRLKPPDKAQLPTGRVRRFMYALVHAKSFDIFILTIIIINVGFLAATWYNEPASYTHMKNVANIVFMSFFAAEAVAKIFALGWVGYFKVGWNKLDFVLLGLGILDLIISMLNSNFLRILRVIRVLRLIALVRIAHVAQLVKSVKGIQSLFTTLIYSLPAFGNVGALIGLFFFMYAYIGMYLFGKVQHGESLSRHVNFHNFWQALLVLMRVATGDNWTGIMFDCMVQPPKCNRSSGGCGTYIAVPYFLTFVLLIYVILLNLFTAVIIETFEKTHEQEEWKLSPQALEDFVTLWSEYDDGSGTILPRHLEELLLRLDPPLGLGPYADNKDVLRFVYDLDIPLVNARVPFHKTAFELVKRCSQATMPEGAIKEHMDRLVNKFFRELPTQDEMLNFSVAITVMKVQRKWRTRMRAAKLMRKKRWRQDRQAAPEYPEIHSNKALLAEKFEAYRETVREAAKAWEAAQISYNRKPDKGETARRTEPRWRFSAKYAPPALVQATSEKTGLLGLGKRVNVNKIMGGLLRVGGLLGGNGGNEGGPARSPQATQSCEQMAMWASPVSPQHGPGGTGPAAAAGQEPSVASPLPRLLRAIALNNGPRLSYNGGGAGGGLLSSPTAPTSEQGSFGGARRQLYGAGGGGVGGGGLSVSGGVDTPSEGLGVRTCTQSLTMSPMVTRRAPGMLSNDGGGGFLPGGANRGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0A8</accession>
    <name>A0ABQ5S0A8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EfeO-type cupredoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006244</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050845">
        <property type="entry name" value="Cu-binding_ET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38439">
        <property type="entry name" value="AURACYANIN-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38439:SF3">
        <property type="entry name" value="COPPER-RESISTANT CUPROPROTEIN COPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="26842" checksum="2AC04F0BB0395A14" modified="2025-10-08" version="1">MQFLLNLPSLQSRCIFSSYSKRMQARSSSRLHISQGRGKCSGIRPWLLQLRPQRWVQQPTCASLRQNSIIADVGDEPCGAPAAHASGTSRRHALSLLAGSAVALTSIAVDMVAPSTAFGALKDYNLDPSEPIEEVAVQLGTEDGQYVFIPSTLELTAGKVYKLKLRNPSTSTHYFTAFEFAEKVFSIMVLAGDPAVEVKGGIQEVALKAGASATWIFIPIKPGKYPLRCTVKGHDAMVGQIVIKRSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0F6</accession>
    <name>A0ABQ5S0F6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_006313</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF37">
        <property type="entry name" value="SI:DKEY-61P9.11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="22"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI63375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="171" mass="19123" checksum="2FA9F39FE5BFE8E8" modified="2025-10-08" version="1" fragment="single">FSSFLQWADQPIYVLDKDNNLDIQSLARRNALDILAVQSEGPFLIGGHSYGGAVAMAIALLLEEWGHDVGLLVIMDTPRPEQVRALQPDATECTEADALELMEMILGALGRDAIGLGSSIAHPRESEEWKSMSLHQKYEFFAPIWRVMRDDAMPVEEVRRQVEYVALAVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0W9</accession>
    <name>A0ABQ5S0W9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U2A'/phosphoprotein 32 family A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006052</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="267"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="354"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="420"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="473"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="621" mass="64139" checksum="B130C7C740015F5C" modified="2025-10-08" version="1" fragment="single">MVKGTEQPKAPSSKASTQAKATTKASVATAATKTGSPAAAGAGRTQPTKAGQTSSGTKGNGSKAEPQPPRPARRAMDGPELAEPTEVEIEAQIKQLLNSADASPPQELNMELIIRSHISNDPALSAARRTASEAFVQQLTHIRLDRQRLEALNTDVLRQLSSATHLFLQHNRLASMAALSALPRLTFLAMGHNRIEKVEGLAKMTELMFLDLSHNRITSLDERSLPPSLRFIKLEGNPCATGHANRYCLRRALKARCPQLRHLDRDLDENGEEVSDEDDEEEEEEKEGDGDRAGAKVMTGGATAASITDAPGAEPDGGASDLLEDMLDMLDDLERKAYKSATARSGALLGASLSRSTELHTSIQLRASGAGSRPGTAAGTGSRPDTGSGSAVAAAAAAAASRPGTASTPPRPRSSGTGGSAAATVAMSATRGSTAEGVPAAVGASARSHSPGPAGAGTRPGSGRLSFTGGGSSRPSSAGSGAVAAGRLGPGTPLPPPPRTQPSGGTPQQRAAALERQVEDDLGMLQARLQTLLEPASSSTLTQDAAIFDARQLIHSQMDRMRGQASERISAQREADAAVSDAIRRLRTSSTLATLRDQINLGPSRAAAARTAAAVAAARRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S197</accession>
    <name>A0ABQ5S197_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Adenylosuccinate synthetase, chloroplastic</fullName>
            <shortName evidence="8">AMPSase</shortName>
            <shortName evidence="8">AdSS</shortName>
            <ecNumber evidence="8">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PURA</name>
        <name evidence="11" type="ORF">VaNZ11_006500</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Plays an important role in the de novo pathway of purine nucleotide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 10">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Plastid</location>
            <location evidence="8">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="8"/>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002223">
        <property type="entry name" value="PRK01117.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="5 8 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="64"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="65"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="90"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="351"/>
            <end position="357"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="383"/>
            <end position="385"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="466"/>
            <end position="468"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="483" mass="52448" checksum="88C4758822C5DF39" modified="2025-10-08" version="1">MLSSHGLKPGTSAREAGRAPAAALSYARLCSARTQSRSSRVAAAAVAAERLDAQVCVVLGTQWGDEGKGKLVDILAQQYDIVARAQGGANAGHTIYDDAGNKYALHLVPSGILNRDSTCVIGNGVVLHLPGLFEEIENLKKKGVSVEGRLKISDRAHLLFDLHKEIDGLREAELAGAGKQIGTTKRGIGPAYSSKATRNGLRVCDLQKLDTFAEKLRKLSLDGEKRFGSGFVYDVDGDIEKYHELAKKVMPFVTDTVEYLHTAYDEGKRILIEGANATMLDVDFGTYPYVTSSNPSIGGIATGLGLPPTKYDALVGVVKAYTTRVGAGPYPTEVFGDLAERLREIGREYGTTTGRPRRIGWMDIVALRYSCRINGLTHLNITKLDVLSELEEIKIGVAYKTRDGQTLRSVPADLETLENVEVVYETFPGWKSDISGVRDWKDMPQAAKDYVQRIEDLIGIHCKWIGVGPGRDALVVKPAKGKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1E4</accession>
    <name>A0ABQ5S1E4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_006731</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0229">DNA integration</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="11"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI63693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="220" mass="24585" checksum="17226E9B68F87E07" modified="2025-10-08" version="1" fragment="single">TRGTHKSTGHKSDRPMELIHVDVCGPMPDNTPNGSRYMTVLMDDCTKFSAVAFTNTKEAVKDKLVTMINQLENMCGHKTWEMRSDRGGEFVNSQLQAFFASRGIRHGPTVGYTPEQNGAAERLNRTLLEKMCALLLESKLPQKMWAEAAATANYLRNISPADGISRTPYELFTGKVPEVDHLRVFGCTAYVHVPKAKRNKLDPVSRRGHARTIQTLGRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1G7</accession>
    <name>A0ABQ5S1G7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_006823</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044554">
        <property type="entry name" value="ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014786">
        <property type="entry name" value="ANAPC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08672">
        <property type="entry name" value="ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01013">
        <property type="entry name" value="APC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="7">
        <location>
            <begin position="18"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="331"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI63750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="493" mass="51818" checksum="747F5209256438BB" modified="2025-10-08" version="1" fragment="single">GAGVAGSVAGSSSAADVVSMLTGVFGSPEFFINDYRNLLGGQLLYRSDYDTVREVRTLELLKMRFGENALHACEVMLKDVADSKRINHRVHSMPSSGTSAAPLPASPIGTAVAAAAAASAAVATAAVAAVRGTPGGRTTRQSQQRSAAASAAGTAAVGSASGTPQVQTRSRVAAAAAATTAVSSSPKPSETLVKASRPEFDLLSATIISYLFWPRQEDRDKGAPDDPATGLLLPKEIRSVMETYEQRYNHLKTPRRLRWRPHLGSVDLDVSAGGVTASFSVSPLMATLLLAFRDRASLSAGELAATVGLPAVLVRRKMLFWVNHGVVVEGRTSRPNGGGGGSSQGPSNAGVWGMAIHGSGQTTGAGDSHYQQLVYRRAEVLPAELQGAKLTDCGAEDEAPAQLPSAADRAVEEIGPFENYIKGMLQNYGGLPLDRLNHMLRLFVVSTPKYDKTPEQLQAFLQVLIAREMVVLDNNSIYRLASAAPAAQRGSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S1W0</accession>
    <name>A0ABQ5S1W0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="311" mass="34031" checksum="7FEB6F4050C30A61" modified="2025-10-08" version="1" fragment="single">MEGPGLSSRATCRACLVMEPRDPVGGLFLRSSSPLQQPPAEFSINEVPIHRCWCRREVVSHRIVISYPIELPEFEEEAFEEQGEHEESQGGEGFSTSAAPSCRAWAARKCAGQEEDVAKGFRECSLLAEATAAPNGVAAVMATVSSEGPGNEAGVDVVLPVVMVKNENEEEKQEAMTALRVAPEAPSATPMSAMHGSSADVAVMFKGDTWVRGPSLQNQFIKLLHQQHPQTPSETSETRSSSRSEAKSQIPSQSNGETERLIRSGVINRRLRSVAFAIDRDDAGFTAFKRLPRRQPVYVRLPLSRMELLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S221</accession>
    <name>A0ABQ5S221_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mannose-P-dolichol utilization defect 1 protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_007016</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MPDU1 (TC 2.A.43.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016817">
        <property type="entry name" value="MannP-dilichol_defect-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 LEC35 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226:SF2">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023381">
        <property type="entry name" value="MannP-dilichol_defect-1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI63895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="24825" checksum="68EF5059D73D24FB" modified="2025-10-08" version="1" fragment="single">MADLFNTTTFVLKHLIETRTLPALDLIKLLISQSLGYAILGGACISKLPQILLIEQAGSAHGLSKEMFEIETYTFLVTALYGYTRQLPFNTYGESLILAMQNLVILGLVYSYTRTPALRRLVVWGAYVALTVGVVTGNITKNTVEVFAHTNTGLVIFSRFPQVVKNFTARSTGTLSGITAAINVLGCVVRIFTTLQAGGGPAMLRSYTVSLIINATLLLQIIAYRKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S267</accession>
    <name>A0ABQ5S267_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_006299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI63366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="164" mass="17262" checksum="860744168C373A18" modified="2025-10-08" version="1" fragment="single">QVALHLSCKADVSLSPVLCGNDVMGPMIEMVDLACRRAAAGEVKDAWILHGGLCAAGFLSKVLVARLRSNDVQLAEVLNSDLLIKLVTLVGHSADAVLVAVNVMEPCYTEPITPEGLGSGLPSTGQRHVFHDDGSIQPKVYRYPNLPGPSTVHPRTCAAAVATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S285</accession>
    <name>A0ABQ5S285_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF126">
        <property type="entry name" value="CONSERVED TLD DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="332"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="563" mass="59007" checksum="F471B8746BFBCDB3" modified="2025-10-08" version="1">MFSWLIGKTTKSSELKEAESVFTETELKEIQKRFSEEVSRQDATTIGRSARAHGSEALDFHGLGALLGPVWKCLDPLIASHLQLQLADKADGKVHLSGLIIAAARAERLGEQASLEFSYQLLSADGTGAGYKALEAAALSCLRLVGAVLWPSSSLNPQAVGAVTTAQPGTSMTSNSSTGNISVGSTDLPDQIRSRNDQRIDNGFADGISSTNSTSNTSSSNNHLQLHLTAFCQGALMLAGPEGAASPVRDLNLSYDGYCKWTKKCPALHKTLCSLLRQVGAPPAAATAATSAIADQGTAAAALAVSAVAALGKDPLRLPLLFQLGKLRACSTLLQPVWTWALASRLPPAQRVEWRLVFSSQRDGKSFNTFFSRISAAAGPTLLLLRDKAGGLFGGYASQPWIKSGRYFGDAACFIFSLLPALQVFPGTGINENFQWCGVGFSQLPGGIGFGGATAARGQFALYVEPSLDSGMSRPIATYGNTPLASEQVFQIDTVECWLLQPPEEDPSGSSVTGAHKPGGGGGGTGGAGRGSILGMAAEDRAIMELAGLKLHSAGLKYEQLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2B3</accession>
    <name>A0ABQ5S2B3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_007103</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45764">
        <property type="entry name" value="BZIP TRANSCRIPTION FACTOR 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45764:SF38">
        <property type="entry name" value="BZIP TRANSCRIPTION FACTOR 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="246"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="331"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="432"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="465" mass="48853" checksum="79F57D848573B403" modified="2025-10-08" version="1">MNVMERQTSVDDLLGGFWRLGQAGMGRTDSEAAFQEFLKRIPSTTNLAAAVAENAGATSSQPVASTSALPSVVLSDVVTSAAAAVGGIPRVPSLELLRQLVHYPGSASSVPAATAAPSIKAEALPGSTGASAFRVPEPADVLKATGVPPLPAPVALPAAPPLPAGYPLPGIPVLSDLASLAAVGGAALQGVPHFAASPSPLTAALTPAAAAAAALQLGQLGSLGARLTSNSTAAGVSSDKDMDRVDQRRARRMLSNRESARRSRRRKQEHLCKLEEERNVLEDERRDLEQQINTLERRTQSLEDENKRLKDENERLRDELRFLRTEISDRKDRSGYGHYGGRGRLHVDSDDELPAKRHRHSDKSSSEEHPGTTVSELETDKRSRNLVAAGVNGTHSTKHGAAINGRLGLGSGGGGAGFRERSGSGAGGAGSERGRADVGKVSRDGGRKLDRAAREARDDDDDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2Q4</accession>
    <name>A0ABQ5S2Q4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GAG-pre-integrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF28">
        <property type="entry name" value="TRANSPOSON-ENCODED PROTEIN WITH RIBONUCLEASE H-LIKE AND RETROVIRUS ZINC FINGER-LIKE DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAG-pre-integrase" evidence="1">
        <location>
            <begin position="68"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="189" mass="20838" checksum="63DDC08DDCE4B86D" modified="2025-10-08" version="1" fragment="single">VFYVPGFKHNLVSVSSLTDKGFNVLFNQSEARITRGNISIVAAHCTPGLYKFMSSASQVAADFASLAKFPDAPTFLASEAKSASLWHRRLGHLGLMNMKRLVQEGMVKGLEVTDKELREFEGRRCEPCILGKHARDPFPASEGKTQKALQLLHLDVCGPLPLSSCGYKYFVTMLDDYTGASLVQPIKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2Q9</accession>
    <name>A0ABQ5S2Q9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Queuine tRNA-ribosyltransferase catalytic subunit 1</fullName>
            <ecNumber evidence="6">2.4.2.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Guanine insertion enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_007374</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>guanosine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:16633"/>
            <dbReference type="Rhea" id="RHEA-COMP:10341"/>
            <dbReference type="Rhea" id="RHEA-COMP:18571"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17433"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:194431"/>
            <dbReference type="EC" id="2.4.2.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of a catalytic subunit and an accessory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the queuine tRNA-ribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.64" evidence="6"/>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00168">
        <property type="entry name" value="Q_tRNA_Tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004803">
        <property type="entry name" value="TGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00430">
        <property type="entry name" value="Q_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43530">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43530:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="8">
        <location>
            <begin position="37"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding" evidence="6">
        <location>
            <begin position="271"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding; important for wobble base 34 recognition" evidence="6">
        <location>
            <begin position="295"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="412"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="423"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="434"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="115"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="455" mass="49548" checksum="4F2F4609469D1426" modified="2025-10-08" version="1">MFGRRFHRVIMASGSGQALPSSDSPALKFKVVAVQGRARSSRMTLPHFTAHTPMFMPVGTQGSVKGLTTLQLEELNCHVILGNTYHLENRPGSGLVAEMGGLHDFINWPRGMLTDSGGFQMVSLLHLAEITEEGVTFQSPVDGSSMLLTPEQSIAVQNRLGADIIMALDDVVPTTANDPARFEEATHRTTRWIDRCIAAHSRPLEQNLFGIVQGGLDPRLRELSMQQLVARDLPGYAIGGLAGGESKDDFWRVVSQCTAGLPPGKPRYVMGIGYPLDIVVCSALGADMYDSVYPTRTARFGVALIPEGVLKLKNAANARDFRPLDPSCSCMVCRRYSRAYLHNVVTRGISTAAILVTYHNVAYTQRLTWEIRTAIEEQRFPEWVRSYLKTMFPKGDVPRWVREAMAASDISLEDAVTGPGSGTTSRNTTNPATATPPAPVPTDPLPISAAVRASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2V2</accession>
    <name>A0ABQ5S2V2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="102" mass="11212" checksum="E719D204B9E43FBC" modified="2025-10-08" version="1" fragment="single">GTLVRWTGQRGICAAPKSCTARFSSFGSSSSWRSSSSYQQASSAASDRGGGLHDPWKLQQLQSQVFELKQRLVEAEVQRQAADKVAAEAQLVRQKLKRTLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S322</accession>
    <name>A0ABQ5S322_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_007527</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF45">
        <property type="entry name" value="RNA-DIRECTED DNA POLYMERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI64302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="46428" checksum="35644C53D22FC048" modified="2025-10-08" version="1" fragment="single">MSEQLAIHRRCRRQYPGTAACVLLPAFCRRKAEASLRGMRRVHRFEPGTVLFNGVDREGTLAPWPGTSYSVDVWYEAPDEGAVGYGPALSALTLSAEQSLVDNRVPVVQIGFNGRTLKALVDTGASDDFISVAEVNALGLTPESSDCPQVTLADGGKHPIVGRITLSLSIGPLRVVTRPYVLQGLAETAPYIMGSSTLRQYRARIDMETATLDMCKGTLSIKVPFLSFKHVTPKEGEAEVVPMVNFAVTAMAAQIEPELVGRKEAVRLLRKGAHGLLLRPWVGCCATTAQISVDPEVEALLSQYEDVFKDIPGLPPMRPVDHTIPLMPGAQPTARPMFRLSPLELDEVKRQVTDLLAKGMIRPSTSPYSAPILFVGKKDGTLRMCIDYRGLNASTVKNRYPLPRVDDLLDQLKGSAYFSSIDLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3I0</accession>
    <name>A0ABQ5S3I0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription factor TFIIIC triple barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI64018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019481">
        <property type="entry name" value="TFIIIC_triple_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10419">
        <property type="entry name" value="TFIIIC_sub6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor TFIIIC triple barrel" evidence="2">
        <location>
            <begin position="24"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="160" mass="17785" checksum="3A10078ED9720CB7" modified="2025-10-08" version="1" fragment="single">SLRILVTPMACVEGPPEVVLTEGDEEVQYVLLDLPRDVAVWLNPGEKLVIQALDSDTPVIKLQNGIVLQGKYEEHLGDILLLDKELALSEQETADGEGVGAEQELTQQQQKPEQEAPAQEQQQTPHRTPQQQAPQQHPCTVRLRGQTDKVLTFKRVITPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3J4</accession>
    <name>A0ABQ5S3J4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NELF-A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_006933</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000016">
        <property type="protein sequence ID" value="GLI63831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056557">
        <property type="entry name" value="NELF-A_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23553">
        <property type="entry name" value="NELF-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NELF-A N-terminal" evidence="2">
        <location>
            <begin position="28"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="451"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI63831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="638" mass="64078" checksum="A2A4651A1F324CB1" modified="2025-10-08" version="1">MEGPGRSHRGASTQAATGTSSLEPHDHTRLWLQSKLSGDWSAQAGASGLTEERLNALSRCFGTLDSMVKVRLLLAGLFLPGRERSRLQPQLLELSSQAASNQDEWVRATAAVVGDFSGPMDMRSLMAQFPLVRESVLRFSSHLSEVQTPTSLLPQTEQYMHGDISRLPAVGSSGAAAASTSTASTSPPSARPHFRLREAPHGSLADLGQGLAGGPGGLAGAAGGALMLPGAGAGASSLAAAASLGLDSKGSLGSGAGSESLVRRSLSQVGGGGGPSETSSILRSRADVFHGKELKQGPPTPSGKGTPPLAPGLPLRLSRSRSLVSPDQQPPISESPSLGAPAGGGGAIAAAAICAGGSLPFSAAAAFNAAAGTATPPPGTTAFTAAHVGPSATIPAHSAIAGGPAAAAAATTTLPPLPPQQPQSHAVHSDRAAGPAAAVAAASGPPPPSSTRPTSLQGGSVSSYSAGAAPTQQQYPLAEAIIAFAAAAASGEPPATFGPLTIAANTAAGQLPSHVPASYAQHQHQKHLRGAQADHLVKRGYSHEREAGVGEYNEGAGRRYGDLEGRPSPGAVERMQLGRGLPRQWSASESGAGGIGWRAEEASDAAGVGGSMEVMGRVELVQEDDDEWDAKTPRPGQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S456</accession>
    <name>A0ABQ5S456_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCR4-NOT transcription complex subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007216">
        <property type="entry name" value="CNOT9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12262">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04078">
        <property type="entry name" value="Rcd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="33146" checksum="14AAC79AADC3357F" modified="2025-10-08" version="1">MSTTSLSDVQQVEQYVQHLINPATRENALLELSKKRENFPELAPYLWHSFGAIAALLQEIVAIYPLLSPPSLTAHASNRVCNALALLQCVASHNETRALFLQAHIPLFLYPFLQTMSKTRPFEYLRLTSLGVIGALVKVDDTDVINFLLSTEIIPLCLRTMEIGTELSKTVATFIVQKILLDDVGLNYICATAERFFAVGAVLGNMVVAQAQMVDQPSQRLLKHIIRCYLRLSDNPRAREALRSCLPELLRNSNFTACLKNDETTRRWLAQLLINVGFPDAAAALGALDVVQPTPMTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S458</accession>
    <name>A0ABQ5S458_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_007896</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="53"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="458"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="624" mass="67720" checksum="8C64BD7B89FBFFCA" modified="2025-10-08" version="1">MYSVLRYLKMRLMKQLLVNVSERRMPGAEAQDDGGSFFDMEVMEPQAGTDPRFPYCSCYRPPANPSPYSFLPTITVPAKGQYCFTLRVNTTGCNTGRCCDVDLKKIEFDVYGKCNVKSAGVKATVAGRFTSTGPNIYIFKMDDGTLKYVLRLTNLNLGLWSDNTQICLNLTAGTNDAGCDTLEELCVPPTAKSPPGTCLATFMNNNNSTDACCPTSVVTPPKPPPPPSPNPPSPLVPLSPEPPSPPPPSPPPPPPTPPPPPPPPPPPPPPSPPPSPFPPVISPPSPLPPPPPPPPPPPHLPPFEPGTPFVAPCVQCLVITFAPLVLSFPGFSLSNITCIRTMEHIAKNFKLIADALHLTLIGDGFILDANNCNLSSLKVCGTFSDKYAVASNQLLQDFFQQVAEYVVKELQDFVCRQRINIEIIATQDYVFGDDSGDKNCFPLYYQEACSICNRTRPFPGCKCNQGAAITPYYALPEISMEPGRRPGSTLYCFTIQEVPVHALNSTAGCGPADILEKIEIYADEARRRNVVGLRIAPKGGVPRWTSANWGVPGSNTFKVTPLRWNSSMAIGGSICLDLTIPLNTFCLGDRTCFVSLFNQERNCCPTYASALEFEPDFDLVLPPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5H4</accession>
    <name>A0ABQ5S5H4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polycystin cation channel PKD1/PKD2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_007860</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013122">
        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051223">
        <property type="entry name" value="Polycystin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877:SF183">
        <property type="entry name" value="AT14535P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877">
        <property type="entry name" value="POLYCYSTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08016">
        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="465"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="502"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin cation channel PKD1/PKD2" evidence="7">
        <location>
            <begin position="389"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="146"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1099"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1174"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1373"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1484"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1531"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1576"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1644"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1100"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1174"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1185"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1376"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1403"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1430"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1485"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1531"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1579"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1605"/>
            <end position="1618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1668" mass="177598" checksum="F5C2C20776EC4247" modified="2025-10-08" version="1">MEATSTAMAALRQIQYGRIGSSGAIRIEEAVADATDPETAADVEEQLAEAVEEVLEGEDVGTEAAAELEAAVEEELEQVLEEEMLQEGEWGGELLADGAGNAIAEANNRAVCEATERSLGSVARPGSSSLKAGTLYGKDFVRGSRIPTRASSDSGTSFISRMSPASLTPTRVTSKTSLSGDLYSNGSVLWAGGSDYRRPSRRTTSRYENQLVSGVSASSGPRLESAAAVTAATAAAAAAAAAAAAAAAGHAGEDHKALALKLMGQEHEVVAVQSKKGIKAVRKYRARMSLFWGVYETLVCGLMAGGLAVLFVYSISLGAPSQRRYDVYDSLSSAPAHVFMLRRSTAGVNATAMPKPGDPFRWKLPEDTSGLEGAASMYDKVLNMRNMWMLYNFFQGLVLMLLIMRLVHRLSFQPRLSIISGTLARMIPDLAGFVLVLITILIMFAFMLVALWGDVVEELSTAADGVVWAFTYFITALDSSAALDVVHAIHDPNNQANGAYTFMSWVVTLCGPLFFIFTLQHFIMALLAWPFSELKMANHDEPLASQEIAKMTWWLWQIILRQAPSNKALMGTVSRTVPKRRPRAAKSFQLKKSVLTSLQGASNLHRKLVTRITARRRPDVLYKTMEVEVNGDWLSAAELEEILEKLRQRRLQKAAMVEVGPDKMTRGSSDAVLKVPSLHRRFSRNDVVPDPTPRFGLSFEFTLWRFDGSSAARYEEACAENGAHGSLHGRPPMLAWPVADMTQRTASTTDANAAALLEHVVAVNVVQRMGELQLHRVKSSSVVSGLKRQASMGGGKVQHGATAAAIAEGTVGGDTTRQLGTDSGSDGGIIGTTLQAMPLMNLTSTAPAVSMDNRGPDGALPAATAASTITSDINVAPPAPGSDMAPQASLRVRQNQQQPAAVVTESNSVTPDTVTMARSSNILPPGAVAEITESDDPMELALAVSPFTMAAAPASLPSALRTVSAVSLKKIRASRVTFSDGVTDNRLSASPFIAGGGADFNSTTAAETVVTIGDSRLPMPPATSSRSVRSRLRQLLVDLDECDPAAMLPDGTTAGAADTVAVTAAAAASMVPTTIVTAGSMEHIGSTGIRSDPILPKVIESSSSTPVSPNGQQSSQMPQALPQPPQSPQPQTSQQPVINTISQDNQHGVPRLFFDFGISLSVAKLGQPDLAAARKVENRPEHQQQVRQEQQQVQQKGSQGSQLQQQHKSQQGIDSNSRKGIPPDQHQQDRGSHGAVELDELDGDEIIIKVIQALIRHAEALLALLQIQREEQSKLSENLERILATPNGEELKRRLMAIADDRTNAEATTVIQGPADSSAEARSVTASRSWTQLIRMIRNPGGGHAVDAAQHEKDIISTVVSQWRSKHSAAVAHTSVAPSADNNTAPAQLAPRSRGWGARRRTAAVAAQQQQTTGKRQRMLGRQPHISAPRDSNTTSNSAAGQYNPPQPNVHRLSPAASSGTGDGSPFPATLAAPLVDNKAKNATSSLLSPRSPSSSAIFTDQPNEARRVINSREPLSIPAALLPRPLETSASSVSASSPSIMQKDSNGLIPTHPAAGPVMAVQHADSRQAHVEDLGQAGSLTSMTAQRRPSGPYSRRLLPPLLPPQSVQQQQQQQQPLHGEQQWSLRSSSSLLMAVQATQAPRVSLGSSLNYSAPSADSETSSRPSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5H8</accession>
    <name>A0ABQ5S5H8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adhesin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34094">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34094:SF1">
        <property type="entry name" value="PROTEIN FAM185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="571"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="671" mass="67530" checksum="435B94CA92FE266A" modified="2025-10-08" version="1">MRTVIRSALIPSYERAAAATAVISSTPPILPLPLLMLSAPASPRSFQRLSAAVWEVYHLLHPSSTSPSHQLTRGFRPIHTSSSKLRPESSSSSSEAATAVPSQSTSLHSKLHQCVSSADGVRSAVTEVPIVCGSFFRLVAGKSAVRIEHFAGWSDTATLEGQTAGGGLWRAVPIDTASVTPSTLASVRQAYEVRQGVDGSKSGTAAATAVAVTTPPSDGAPGLLQLTAPEKWISMDVRTSGCSVAVSKIVEADLRVATGGAAVTLGSVRGLQVDIDTSVARSEGVDGHTRICADAAGSHDGVGDSCDGSSGGGCSDDGSSTAPGAAVMGGEVSGTSISIVAAGIINVRRLVGGTMRLTANLESIKSPSLPSGNVSQEAVTAAAAKQGIELGAVYGGRLQISTGGGSVRIGTLDCGAARMRHPSDTSAINHADLAFTRGSVNDSGSGSDADADTNAFILPNTAGGGDRDVSARSSGSGGADILSHGGAVVLDGVEGDLLVDSGGGRIKVLIQAGLRSARLVSGGAPVDVALAPGVALRLLEVHGAAEVEVQPDLQHHLERVLQAPQARAVAVGSRSGSGESSVAMGDGGSSNASGGNSSSGTSRVWVARLVPEPEGGRGTSRAGSGRRGRSGGVLTVDAGGGTVSFRRLEWMEALKAKLQEKHQTGQPQTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S682</accession>
    <name>A0ABQ5S682_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008672</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="355" mass="37746" checksum="81FAEF14D5A99E8C" modified="2025-10-08" version="1">MSHINKACGPLLLRRRSALPVLLTVLAIGFALMAIAQSAARGRFPTLKSACIEVPGYEALPDYGIELRSGFSFPQNDDEGKDYPNASSALLACDSDPTCVGYNTDGVTITQAGLISVAYVSELRTCLWIKIGSSEAYSGYTRRPYLSWSAADSATVFVDTASAAAAKEACDQNPACTAWNTAGQFAIGAVDTQAFPASPGGTTLYLKPVCRDKFGYTSYYGYTLSGLSGVSGLGTGKMPGGGADAADFCRLDYRCTAFSTDGSYAIGGVDLNNVGGTTCTYVKEPCAPIRGFAAVNDQNSHSAPDSRPYYNCFSDVYKACLNDPECIAFNNVRNIWKVEPDDYVDAPGICFYLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6C9</accession>
    <name>A0ABQ5S6C9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_008572</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000022">
        <property type="protein sequence ID" value="GLI65120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12393:SF6">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12393">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI65120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="630" mass="69154" checksum="095D80B4A0881C70" modified="2025-10-08" version="1">MVTQVLCSTSMESFDEIGSRVWSLLLEYIRRNEFGSILPRNFVACNIRVVNKLATTVLRGPEFFTVHISEPCPTDYFQRHWGIPEAAMRIYILDTVRWLYIPEALQNLNMQERLRFVCLTAASGVVANLQVAFEVAGLTPNEAMLEAAAAAGKLESCKWLRAHGCPWGKALTAAGLSGHRDVYEYMLLLGCPCDDGALYAPVRGGHTDLMDWLGLLKPTSATGTVKLDKLMTAAAEGCELATFIELYRDWLGGFMGRLRATQFLGHSTKARILAAAAGSPTPDWRAKVEWLEALGLPKSPKAIAAAVLQPDGLERLLWLRERGYALEIDQCAFQAAGRDGNIALVRYLLSELALYQFTGAAAEAAARAGHLDFLKEMHKVSPLDGAVVRNVVLQAARSRHLDVVTWAVRCPGQGVYDVQNDVGLLPAAAESGSVELLEWVWERNSIRSGDITVAAARGGCKAALEWSAQRRLLDIADQTALYFSAAFNGDLCTLRWLQGHLRGEMPGDIVSHCAQGGCPIASIDWLVDNAGCRIDAWDAAGLTPEQPGVWNVLCRAASVASSGSSSVDSSVMRAQWEHELQLLLQELQHLPGELHHSPELEAELQWRLRQLPTQVQQLLRQQLEQISIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6N6</accession>
    <name>A0ABQ5S6N6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_008428</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI65002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909:SF1">
        <property type="entry name" value="CARBOHYDRATE KINASE PFKB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909">
        <property type="entry name" value="ZGC:136858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="5">
        <location>
            <begin position="42"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="5">
        <location>
            <begin position="465"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI65002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="559" mass="57567" checksum="F6065AB42595090F" modified="2025-10-08" version="1">MADGRLAVLLAHLATEHEGTQSQPGSRVESPWFAASNGPLVIIGGVCLDIQPSSIDVQRGTSVPGQIRQVSGGVGRNIAEALSALLPPEHPSPVFVSLVGDDTAGSFLTASLRRLRLDLTHLITLSGAPTPCISAVLDRSGEVAACIADVAAVEHHLTPERLRECQPKIHQACMLLAEGNLSAEALEYVTRIAAHARVPVFLEPVSVPKATRLVPALPYATFVSPNAAELMSIADEVRRVAGLPLLPRPALPSDAGDGPRTGGGGSGGEREAAGLVVELLRQLAAFAEVVLAKGTSCVVLTLGSLGAALITLTNASIAVAPRAPLEPLDACSRIEPGLAAGCDTGSGSCWSKSDLGDFATAAGQDITVRRLTAGAVSVTRSHPQGAIHAAHCAAAAWALPGCGRMSIEPASREDASCRGPLTRQEAPAAPLALVPSSSWWTAAAGFDVAGDVAAVKQPLQRCMVQVVHLRALPAEVVSVNGAGDTLVAGMVAALLQQEDPVHALAYGMAAAKRAVESYRNVPEIECGSLRQDANAVVATQQRYFFPSTLAGTPATLSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S746</accession>
    <name>A0ABQ5S746_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_009004</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-prolyl-[collagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:18945"/>
            <dbReference type="Rhea" id="RHEA-COMP:11676"/>
            <dbReference type="Rhea" id="RHEA-COMP:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000024">
        <property type="protein sequence ID" value="GLI65484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF247">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="8">
        <location>
            <begin position="374"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI65484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="533" mass="55593" checksum="82FAA639A59A3C98" modified="2025-10-08" version="1">MGKTLAKARKKLAREAQQTSQHGYVGGVHVGALGVIAKRLKYKLPTNPKKKVKLVPIISEALNAKAWADAQELLGLLEQKQHGADVEVPKLGAIMRWVRAADLAGTESIAARLLASIMRVAAGPQPHPASSDATPEAAAGQVQNRAGSDGATCALLPVRRFPPWHPPSTDAVGPSDKERTQLANVKMASTAAASYGPYAGHFAIVPLPPEYAGTNANGSTAGIAAATASSGAGDNSTATALPNIARDLTMFCTEPGTIQFDVSRSPAVRVEVPFVGGAFVLVGVLSRCECAQIVACAEEMGYTVDPDYTFSAAARTAAAGAGLSASVSSAARSGGAGLGERGAAGCVWLADETLQGPLYERVAHLLPQHLCDGDLAGLNARWRLYRYDKGAVYRPHVDGAWPGSGLKNGRYEFDAYGDRWSRLTFLVYLNDDFEGGATTFYTPAQPGSGCCLEAHSVRPVAGNILVFPHGDTQGSLVHEGSTVTQGSKYVIRTEVLYKLAPGVKGGGVGAPQPAMQLMAGRATVRTGTARKVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7M5</accession>
    <name>A0ABQ5S7M5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="744"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="879"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="476"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="752"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="796"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="809"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1084" mass="108134" checksum="BAA75A2CFFFC58E0" modified="2025-10-08" version="1">MEPARARPPATLSQRLQDIAERLSTLAKAELYQVLEGIAPAGEFSSLHRLDPLVNDIVLQISDSFRQAVTKRLLPEACKLIGSRSAALAAAPPWVGAQAPQSPGPTSSNVKQHSHLENDILALGLAPGTGQPLRPDQGPPQDAASAAASSLLQGAVAVPSPQQRHQSNISPPPYTASGNGGGGSSGSGSGGTSASVLHPPESRFAFANVNSDIRRRTTPLSPPLGRDGSYSGGAAGNAAAMADLVSSADPLLLLGIGENGAGLGFDAGSGGGNGSAANGLTVNVDSFLLGSSLAGSIGSAVGSGAGGRATPGAGGSAGGGGGGGVFTPVTSPSPSALSRHLGALSLAAGTPGGSSGGGAASAAAAASRIVSSSISSPSPNPSTPSPYASLPLAIRPGSTLDPGLATPPLSLGGTEPLRGNPQALPTSQLQQQQQQLDRLSAGGEMSPLTPLGPGGNAAAAVQPVPLFGGAPGPSPGGSQQQQQQQQLLRQQQAIQHLQHQYVGGSAAGMVREVGGMAPNGNTGNGSGNAAAFLLQQQQLQQQVQVQYNLQQQQQDMNDYQRQQYNLTQQQQLNTLHYDGSGFTPVSTVRTAFAAAPDGPRASYDGRSPSSGFAVQSMQVPYDVQMMYGGSGVGALDLQQAGAMGDRYASPGPGSAATAVRGGLTSGGGSASPDYGVYSLTPLAGGVGGGMVVDVIRSGSSGGGNAAAVTTMGMLQSAGGAGAGGFGSGVVGIVRGGGSPAMALPASGASGTAVGRASRSGATSAGSVVDSCSSSFSAGPIGPQAAAPRRGSGNGGSTATVQSPFMMQQSSPPASDASGPSSAASVSTAGVTAGGGGGVAMVKGGGVEAAVTATDGSSSLSMAAKLQQVHMDTPALVASKPAPSPIGGGSGRGLKGGAGPKAPADMPGRFRCLPPAVQSRIHALTRDNHVVLLKDFDEKVLSKMTLLVDKFGAAECLAMLDRIEEVLKGKAGRLANGPGYLDVSVSTRLDELKQRASGQVTAPEDYARSTLHSRIYAELRDLITRHGFLQWHHFDQGIINMLKKMPNATALERLNELAYHSFKNVDNPKSCIVSIFTTKPKYVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S810</accession>
    <name>A0ABQ5S810_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="126" mass="14022" checksum="2EB8D804B774ACF8" modified="2025-10-08" version="1" fragment="single">FYVDEFEKKSWKKLWNKESWAAWRSFKYAQPDNPDAGPSEHRIVMEDLNVTDRQQELINALAQRKQIESSKTSFAVPATPVPVGAGGTAQHTPGLGALELPKGTTKSGRNDEPPGTQDGRTSKRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8S6</accession>
    <name>A0ABQ5S8S6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PRC-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36740">
        <property type="entry name" value="PRC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36740:SF1">
        <property type="entry name" value="PRC-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="55273" checksum="756D47FA06D1F145" modified="2025-10-08" version="1" fragment="single">EAWVDASRLEVTCFDLESNGAIGVVPLLTVKQVGDVVLVQEDGVLRQPLDSRFGFNKLLGWEVRNPSNRALGKVRDISFSRETGNISKIEFDDFGLRFLRADFFDRFSVSADAIRQCAYGIVLLASDNVQYQEKQGILSGLLRRVGGAQQLAGLLADGSRGPEAAGLLPQGYSYEQWQNDVRMWEAQTGCSYEEYMMQTGQQQQQPYGMLRGNNALPPGRPQPQVLLPEARQLGMPKYALPPPGTSSQTQPRPGMLNPMQQQPPQRFTGSGQQPGLYGGAYGSSYGGSVDPRFRGPVAAQPPTRVPSPQTRYPAGAGPQQPQSSPAQQQQPQQWQQPVAQPQAQQQWGQQQQQQQQLPQQQNIPQRPMPGSPARNGVMAPGAGVAGPPTADRGSQAAQGQAPPLQQQQQPQPQQQYGWPGPAPGSSGMTAGSDAGRRPAQQPIGENGATGSVMRPDEWLARGPQGQAPGYDQVLEVPGGRPPTPSGIPPAQPAQVLAETPGWDPPGAGVSRQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8V6</accession>
    <name>A0ABQ5S8V6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_010146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI66363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18606" checksum="4DEEBF1AA4F51217" modified="2025-10-08" version="1" fragment="single">MRQKNVECSTAEATRAYLRDVRKYLQLVRGSDRNAVRCLFVRNTLKGRAKTWYDQWTTAKETFTFDELTAALFARFAPEVQNPPHPILRALPIVSKPPWEHGCRPQSRLRQPTGFWLFRSIYQGRGAGQDAEMKALKVANARQHLWELPMCDVPICKAASST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAM0</accession>
    <name>A0ABQ5SAM0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011066</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI66977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="396" mass="44051" checksum="815501A5497C56EA" modified="2025-10-08" version="1" fragment="single">MVARAGSQHTAVEITRLVKLVEYLRQHDAQDSNQTSHCQRIQEWLHNIARQLKKSYPRAPPPAANPISGSAGMEESVVQHNSLPDKSEVLAFVHGLQQSASDALKHDLSSAKCVRLEAAIAIMHALVAVLVSGLYLPPLRMSALSTLMVPGSTSCCNKDCRQRGCRGNNLTQVVRRPKVQHPAMEDDPSSSGPSCSTGPPNNVAGVACMQLELNHHKTARFTRNREPLTVILPPDLSKLIQTYVRHARPVLMQRAPVDMTSSQQLLIDKNGQPFNNGFAMEWRDIEERYGAPWEHIPPQKLRHIHSTTAYTSLVQEVARQLPDLQPHAQVMGNSLRVWDSHYIQRPGSIIAQAGVDNLNKWRKAQLGLNNQVWRKKMVERMLDVEAKGPANEGKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAQ0</accession>
    <name>A0ABQ5SAQ0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_010578</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI66701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="778" mass="81412" checksum="10D75C8D9D1F4272" modified="2025-10-08" version="1">MGCGASKSAGTKATPIDSARISVTPVGPTPRTSTANAPAAAAGGGGSSTPPPDSGSETGPAFAAHENAPLIKRRIISLPKASQRAVSLAWLESFAAELPNDSLTSTQVAEQIVAPAVIQAKRPPSGGDAAATTTAAAAGTYGSYAELLLAGDGTAAAVSVDRPYHYVIHARSRRFVDTVAMLRKHFAAEAQQAQHEGRCAGLTDSANAFFWLDLFAWGGQSCRSGGGGGGAAAVAGDTVDDDDSDEGDDVADADLNTVREAVTGAQSVLLVLDDDVEALRQLWCLYELWLAGVTSRPLRVLTYDLDFETLRYAIADADLSAAIAAAGGPNANRRRHLLRQLLGDTAEGDVSATTAAAASVSRALRDAMVASILAEARQDQQRLESSSLSAATAAATDGDGDGNDASDDASMNADVRWSAAAIFRLDRAARMLEVLGRMAEAEVLFRRVLAATEAALGTEHPDTLTSVANLASLLHDQRRLAEAEPLLRRVLSATERALGVTHHETAASLEALALLLEQQGRMTESEAAFRRALLAYEGALGAANRDTLAAANNLAHVLRLQGGPAQLEEAERLYRRSLAGYEASLGPEHHDTLAAINNLALVMKDAGKLDAAEALYRRALDGCERALGPMHPHTLSAVNNLAAALQSRGQLDAAEGLFRRALVGREGELGPEHPDTLGSVNNLAVLLQMRGHYGDAEPLFRRALAGWEAALGPDNSDTLTACFNLGVVLEALGRPEEALVLYRREAAARAHRGEAGRAALRQVHISELAEALMQPTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB29</accession>
    <name>A0ABQ5SB29_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">VAMT041</name>
        <name evidence="5" type="ORF">VaNZ11_010634</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000038">
        <property type="protein sequence ID" value="GLI66698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="52"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="88"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="386"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="501"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="624"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="520"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="534"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="601"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI66698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="713" mass="74698" checksum="081356663F8CA6BC" modified="2025-10-08" version="1">MGNNQSLPEKVLSAVQRNDVCALQALLSNLNPSGSINDPALRTAVLEYKDKNGRTPLLVAAAKNHYQVLQQLILLGANVHYINPSRDSVGSALHEAAARRHEATVELLLSAGASPFTANAAGRTALDEAVLSGNTGVVRAIEKYAEFSGIVAFKTRTMGGLSSKYKARWAVMMPYFPFGRGREGSSASASGNLGRSCTEMTPKTRRCLWLYKDKFSAAPRCRLWVDGAAVLTNGPAGTEGTLRLHTSHGEPIGDLVASFSHGYCVAFRPADMTPSATSAYHRLVVLLNGSAISGTQLPPQQQQQQQQQAVAAMSAGPPPVMHYPAAGSLVSNPNAVLQPPVQLMPVPTMLPNTGQTPSPSRPYYPVMLPVAAHGAGVAGVSSFIRMSSPPPPPQQVSTSELAVTPPPSSSRGQSLLFPGTAGHCPVVLPPTLHTQRQPLPAAAGTMPAAAHVQSAQGDVIDVSEPGVDGQVVVDMIVERMAALPGESDEAFAMRLASAISASSGGNSQRPLHPQKHFRAEAPSPARSAAQAASDLSTQSTQRLRFSTGEDVSPSLPRANSDQHNIPGPYPPSFDVAGGERGPSGHSQQVRPAGGLAAGQREQYNTSGSGTSGCDNTSFMAHVPPSSCGSRDMEPSAPPADPFAASTARSAPRQPVPPEPEPECVICLNAPREVGFLHGDSVHCCVCRECSKCITAGDLCPLCRQPVERVLGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SBF8</accession>
    <name>A0ABQ5SBF8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010757</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000040">
        <property type="protein sequence ID" value="GLI66784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045716509" description="Pherophorin domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="40"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="2">
        <location>
            <begin position="231"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="383" mass="41194" checksum="6D5DCA37F6AE1B56" modified="2025-10-08" version="1">MARNSCVFAILAVAIAALLAALPAQAGNSSSLPQTNGSLFPPNCNCLRNGSASPFRLELLQPSAIAIQGFFRYCFNLANVGGCSPTQRCCNSTQGVYKIELDINAACKGYLRRVTVNNTNHTAFEFNTNSAVVRVTSLNLTVDAVNNTQVCLFIKASNESCSKLESFCTIGETACRYAIFQTDRTCCPVGVAGLLPSPPFLPMYEHPPPPPPLLPPVIEFTAPPPPPPSSFPNCSCDRAVTSSQYTVKPIADVSPAGDRNLTQICFTINTKASCKNSTSSCCTFTLSKLELDANPDCATALSYMSVDGVIRPRFFQYNPYPAIKITNINKTFDQADGTKVCLFMKPECNSLDELGANKDGTISAAVFNHPSEKINCCPVFTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCC2</accession>
    <name>A0ABQ5SCC2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">IC97/Casc1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_011760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNAI7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031826">
        <property type="entry name" value="IC97/Casc1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023247">
        <property type="entry name" value="IC97/Dnai7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20929:SF11">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20929">
        <property type="entry name" value="LUNG ADENOMA SUSCEPTIBILITY 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15927">
        <property type="entry name" value="Casc1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IC97/Casc1 N-terminal" evidence="3">
        <location>
            <begin position="29"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="689" mass="76895" checksum="F3ED8C6DF73F891D" modified="2025-10-08" version="1">MPPKAGKTGKKKKKSKEELEEERRQAEEAARLAEEERLRAEEAERQRLAELERQRLELLGQFLAAERARLDAQLAELDPLLRQFEHERVRSRNEALEAAEWERFLRCVRVPAPRQRVEVTEFLLRMNESVRDEDSRNIDEAFRACEDCRTMILETRQALLDARYRVVGPAGPDEEATRKVRATPVDWTIELESDLRALFNIINARIDRFTATVLHHCDEYANERNEIQLGHVAGTFKWGVWVNTAKNPRLKVVEMPQLGVTLDIPKQIALANVALRVQHRSGPASDEFFWRCGNQWMAVGGVLVVDLLSLPPGAKKVRGWTLRQVTALASNVQRVPYPIPPAGADPLTWQSEEEPPPMGVTAPLPPDVVLLQDPLQVAWWDEPHQIWNVDGISNVAYDPATNYVSFHTTHLAPLALVMSRTRLLPYKRWNVRPTGGRNGNSAAVTLDCGLDESVVIEVSIKTATLSSPTWPQLQSLLGVAMPPLDLLQALSDRGLHLLPEDRDAVAAGVTAKERSTEEAMCKDLALLGGVFLIASSRWNQTAAPDEALARLSEVVDWEEGGRTLPQHVARIFDKEKEDGERRVLAVMRRGVKGVAFCDALDKREEYPRLPGTESVEAVKACGETIFGEVHASVLALLRGQFAVPGNAESPLALRLAASSESLELARTTSPLFTATLAQTMMALRLFSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCF4</accession>
    <name>A0ABQ5SCF4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_011144</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF39">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="9">
        <location>
            <begin position="46"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI67220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="241" mass="27376" checksum="6161DC3074FE7EBA" modified="2025-10-08" version="1" fragment="single">SASILSLSLYTNEISEDDSFKAINPIICSALSAFQRLGMRVGRMWAFNKGLPVRPGEYDEDQFLGLDYCIYLASKFGMRVILALTNLWPQYKGPEHYLYMATGSADGKTVYDYYADRSTRELVKRHFDAIVNRVNAYSGYSYKDDPTILGWDVMNEPRCPGCDESQISVKLDWLREMAAYLRSIDPNHLITQGSEGYFMPDSATNLHLLNPGAGAQCEGEDWIQTVSMKEHDFACIHVYER</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SCW0</accession>
    <name>A0ABQ5SCW0_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012013</name>
        <name evidence="3" type="ORF">VaNZ11_012016</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-12" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Nozaki H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">NIES-4468</strain>
        </source>
    </reference>
    <reference evidence="2 4" key="2">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="282" mass="29187" checksum="9CF1E615EFBC04B3" modified="2025-10-08" version="1">MDAGTPPLPVTPAYFASCCISGGAIAPAPIANADLAQAVETDASISDASGCFPSSSFITSFVGNTPFPTVAPKSGQPVGGHVKQKYRLVSPLFWLIPPVAAFSSEGGLLCTDSVHALALARAAAGRRRMMAALEEARRRFSHLSRSKLPNSWPSTAPSISTAAQELQFQRQGSSREGATGSRCLDEGSEMQRPMVAPPARLLPNLPLAPRDVGILSVGYAASIAVVPCEASKSVSKVPETQQGRAGEYGRGNQEGQYEMGGRDGGDSDGRQCANAATVGSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SD47</accession>
    <name>A0ABQ5SD47_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024616">
        <property type="entry name" value="Pherophorin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12499">
        <property type="entry name" value="DUF3707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045476219" description="Pherophorin domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Pherophorin" evidence="3">
        <location>
            <begin position="44"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI67825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="337" mass="35361" checksum="51A4615A05C5EAD5" modified="2025-10-08" version="1" fragment="single">MKATLLLVFLAVAIGAAMADELESDNIGRELLQVSPVIFRGNSFPFYSCNASASGSPCYLGAPTTRPINATLSDFCFTVTCPGCNRANPCCRQITANVHKIMFGLKHQCKTAFDGIRSPMSYNGKPLSAGVYIDVVPNNVAMRIRWTSIDLSISELSDSRICVRAKTPCNIANRLFLQNSLGYPLVSLIDGTGEKCCPFRFPDIMPPSPLPPSPSPPSPPPPSPLPPSPPPPSPSPPSPPPPSPSPPPPRPPPPKPPSPSPPPPPPPPPPPPPPPFPPPPPPPPPPPPPPPFPPPPPPPPPFPPPPPPPPPPPPPPPFPPPPPPPPPPPPPPPFPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDJ9</accession>
    <name>A0ABQ5SDJ9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_011433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="337" mass="37198" checksum="D6C1A7B1C94354E2" modified="2025-10-08" version="1" fragment="single">MEYARCGGSWTCFNSRKTSVAVSARQSIHQQEWRIDGPAPGGRGRDQGRGEAARGRGGRGAARGRGSRPPTPRVPNRLFDDDQGDHSGPNSRPTRIMAPRRPPPDRSAQPTSLPLSSQRAPRLSPPSRDTQDPFTNLHSWIQIQHADGVVVRRETWEKLLDHIRTSGHAREALDLMLRDAEVRDATGWEWATHGYGQTAFQLVQVASIQMAAVVGAPELAGQVLERREDLHLTLNKDFVPSLMRKVAAATATSESADGIGLDNGNIDARIEVIQQLYEQEAALRGANSPEAAYELSRVLRRAGDVRGVSSLHLQIREEALAVRDGLLEDMERWLAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SDQ5</accession>
    <name>A0ABQ5SDQ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_011817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000078">
        <property type="protein sequence ID" value="GLI67583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="210"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="290"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI67583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="36081" checksum="108B92D92C528308" modified="2025-10-08" version="1">MEDVLKLLAAGADVTPFTFPAGILGAGPGPGLGATNLDLAIALQTGNFPTFLMGSLGSGAGAPLPLPQALALPSLELPKLLSLDVGLEGGPGPSARAAIAAAGAENDYNSDPSGSGGSGPSRNGWTRQQQLQELQQQQQHLEAMPPPTVGAAHKRDLEQPASVTRNKRQAATAAQEPKVDAKAESSRGAPATVVAAEAAGVVAAATDRAEARRAEARQHQQAADECNSDGSDTTSESAGGGSRRPAPSRGARRPATAKDPVARAQMRQLKKRDLNREAQRRFRDRQKAALALLESTTQSQQREIEALRLTNKLLEEQYRVLMEQYKALQLRLVAMQMKTQGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEI8</accession>
    <name>A0ABQ5SEI8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_012287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1002"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1138"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1393"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="641"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="652"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="691"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="751"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="768"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="878"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1002"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1158"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1184"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1446"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1476"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1544"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI67955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1685" mass="179683" checksum="CF09BCD4F7799D42" modified="2025-10-08" version="1" fragment="single">MGFLKKFKEQKQDGPDAAPESGPVSTQPSIGPEDFLPPEPVPRTTRSRSKASSAAPPASLDVASPAKGKAAGRGKAKEPQQQPAVEAKPPAGPTVSPKCAKVKAASPQVSPKGAASTIPVAKQGAEDAASQLDALGSGALAPLAFDSVLTSKGSSVRARAVPKVAPSPRKETRGKGVNSQVETPKGGSSTESVQKLELKEVQALSDPFTEQPPAAADVIPEVKEEHDSTADASQRKVVSNIGPNSTRAVDSAVPLGPPSVHTETMAGLLTVSEADMPRDPRKRHRAQMSENPLPSAERLDSAAVARPKTQPNASSVSTVQPQLELQQPAQQQAPQHAQPKPHAMSQSQQAAHPACNGQGAVAASEPDRQETAAAQPEAIRVVSVLTLGSAHGTIGSAAGASEHLGSNLENDDATLPPPPPLPLPPPLPPHLACEHSTPHASTSGGSPLAGRDDACGRSSYVMVGLRETTTAAGAAQAEPAGACGSGGSGADRERDRERHRQDAERTREWSDRDREKDRSRHQPEADSKGRGGADGRASTPGRRDKDRDGGRGENKAAVRSRSRSRDRAAKLSGDTDAAKDKDRPREKDTSARSEKDNERMRQKERDKEKQREREKEREKDGKDKARGVDASTVRPPEGNAANGQRNIVNDRSGSDRHKGSGGRERASGRSRSRSRDAKRLRAEDSKAAMNGGEASAPSAPADGSAAARGSGSNQSRRDSGSSAAPSTRGPGGCNRGGVSETQAPTASGAVRKNDTTEDVRDAERAQEEGAAAENNLEFGRAGADATQPSPSAPATSAEDIIALGPGPADGSAPSVGALGSGRGKREPIVWNPPALPGAASPRGHKLLSEPAAEPGGSNSGDHVEQLAEAVRAPLSPSPRRTYTSSDSEPTRSRSRGYSSSDEDDDAGSMGKRDGHTTGNTAGADGGVVEGEFDNAENEHEEVLLEYDFDLMEADLIDEHLDEHVRTQDEVAATVTAAADGGAADQALAALKLPDSVVAKDAAADGEKLETKEAASSGRFRNGTTGRVAYEDMGAPGPDLSRDKQVAHVTAGLVGQVGEEVLAKLRKLLNDMSALDLSTVRPWALRSLLAINDDEARWRAAQALLSIEWSTANNPGGVLHSRLQRAVEMERMALLRSGRVARAKRARSNSPGGVRPVAKRVERPNDTRPVRTASAEGKRRRSRSHERNRGGGSNDRGRSREPRETRDGGSRPKSRDADEERRRDRQRAASKDRDVRDRERDRDLRDAGLPRASLREPDVKSRRWDDVMRATMRGCIVKLPRAFVEDARRVLYDIDGLDCSQIDANAWDPLLMLPNCEQRLNALRTLRNIRWKERFNVSATVKGTLLRCAEQVQRDMQRRDMMRPGMPLIPMGPLGLIPPGGGVGALPVRRDARYGPPLGAPPAPPSLLGSRVLPPPPPLPGGSALGMPATRYPPVPEQSRLASMPSRVRDRDRDRDRDRDDRSKHRAGAGGVPVSRGRSRSKSRSPSRGRRGARRSSSKTRSPTKGRIKARSKSRGRSRSPRHASHTKSRSRSRERRNDAREPSSSRSGASSKSHSPTDRRGTKRSRSPSQAPADGGVAEEAGSGGTTSRPLSSEFKDRMYKYLKSFGKLDLSGVDDRVFECLGRLPDDSARMDCLRRLSGVQWHAVSGAQETQKRLHALLERGTNELLKGHGVPTKKSYTGGSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SEU4</accession>
    <name>A0ABQ5SEU4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_012309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI67973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI67973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="271" mass="27614" checksum="39EE2821593D82A5" modified="2025-10-08" version="1">MATMAANSPSKKRVACETAASVCQLSKKPAKDAAWPAAGPMTGVPGWQYPPLPMGMIPPVPYCMPGNPFFPFMGMFPPFWLPAAAMAAATMPALGAASAANVGMTAAVPGIPGAPMLQPSTSTMWPMMTCTAAIQPMTAATYTSSGISSAAAIPSASCTNSVPTVGVIQSPPSPPPLAASPVSVCNSGLQQQPEDLEFANFIDSFLLSEDSEDVLHGSGGDIDEPRIADLVSSGASNSSDATTDEGVCLTRSDSYCLLSLLGPDEIDLPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SF99</accession>
    <name>A0ABQ5SF99_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_012734</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF155">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="29"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="233"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="445"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="599"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="601"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI68334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="758" mass="81731" checksum="9412BB3DFAC555AC" modified="2025-10-08" version="1">MEAPETAALNALRSVFGLTFFRPGQLEAVLATLAGQDSLIILPTGGGKSVCFQLPCHLRSCGFTVVVCPLIALAKDQVDRCDERGIYAERWNCEVPESRKASIARDMVSDEPSLRLLYITPEGLRHPKLLESLKEACVKNNLVSFAVDEAHTVSEWGHDFRPAYLELSCLKQEFPSVPVAALTASCTSKVSDDIISLLRLHSPRIIKGSFNRPNIAYQVRFKELLGEGSDTAALEDLIHFIRQRSDQCGIIYARLRATCDWLASALAARDIDVGCYHAGKSTESRARVQRDWSSGGVNIVVATIAFGMGIDRADVRWVVHWNAPSSMEGFYQESGRAGRDGQPSLSIMYASQADLQAAHKMERGTRTGAVAEVASYVHGRSCRRKCILNFFGEQSGPCKSECDEICDYCADPPAVQRALTQLEKRETAQGSKAAAVRGRMMGIRVESDTDGGNGADPESHSAGSTQFPRTARAAWTRATIYDGTPSAPNTTLKDPGDNDLTLHSQESGCILQQNLKLYTRTQFGHEGPDGHTGVLQVLRRPPVLKRPLKAAASAAAVPVGAGAVCPDTYQQPPSSHGCFQPEARGLGVDEVDLGLVRPSHTLGSKSSHSAAGRSCTGNLSDATGAEIDEIQPRQREVKKLKASAHLPFKAPARAARPLLARLSGAGQAEQGICWDGERNTDAVGEGRWSGVEDAAHCQLEAGPSQRLQVAATSVNGLSLAPPMETDDTDSGKDAACPRAQLLVKRRLPFKPPVRIKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SFM1</accession>
    <name>A0ABQ5SFM1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hikeshi-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_012876</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OPI10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000079">
        <property type="protein sequence ID" value="GLI68454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048364">
        <property type="entry name" value="Hikeshi-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008493">
        <property type="entry name" value="Hikeshi-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031318">
        <property type="entry name" value="OPI10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12925">
        <property type="entry name" value="HIKESHI FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12925:SF0">
        <property type="entry name" value="PROTEIN HIKESHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21057">
        <property type="entry name" value="Hikeshi-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05603">
        <property type="entry name" value="Hikeshi-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hikeshi-like N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Hikeshi-like C-terminal" evidence="3">
        <location>
            <begin position="131"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI68454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20967" checksum="10400460C32C20D2" modified="2025-10-08" version="1">MFGLFFVGVTGPITSNQLVQVDPTHWVLNCGNITTNYTDLKEVALFLLPGTSFDPNAALGLYVKAGAAGEWAYRGCVHNAHPSEVMPLQWPLNDDGSPLPSTLGPVQIGISIEPGEQIIQKEGSAHGAKEEFAKRVGLDLFRFLESFQTQQMGDHIVVPMNALDRWFVRFSEKYRRDPDFLTRRKEAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGF1</accession>
    <name>A0ABQ5SGF1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_013541</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="16"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="348" mass="38047" checksum="5DD814B25FCB387C" modified="2025-10-08" version="1">MASVEVTCDHWISKVLMVAAYLTLNISLNMVNKWTISIYGFHFPVALSIAHMVFSFVVLTPFMCSKHNREQHYPTLSKQWPGLLFISICFAMNVGLNNISLLSISLSLNQVIRASIPVFTALGAIVIENRPPSRQEFLSLLVLVGGVSIAVYEGSTTKTSFTGVVLCIIGTMCNGLAMSSIGRLLTEKLDVLRLTFYTAPMSAFVLLPFFNKLEADAFYKYWDQGLGFLGIIFLGCLNALLYNLIHSWVIKATSSVTTTVIGEMKIVLILLLSAIVLGESEVWTVKMMIGCTTAILGFCMYSHSRLLSGPQMAPIVIKGVPELAPSITDSIRTPLMSGGILAMKQGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGN1</accession>
    <name>A0ABQ5SGN1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_013676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI69122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="479" mass="49791" checksum="01F4FDD1DA23136F" modified="2025-10-08" version="1">MDAAISATPPLVKGQLARAAVVEAPEGPHLVDSLSNKSPAQIGNIFRGLTAAAAAAKQEPLVVGGDDRGPGGRGRGGQDLVEQVNAALHAAFDRPDVPLEAYREKAPLDQDQQQQPGTARTAPGSSIEALRSADILHDKLVDQARLLVWHFWMVGRLQLTAAAEVAAVTRGMWSWLLRCVAHLAAALGLLAAMAAAAAGGAFVAAGAWALASGVRQLRSASKLEVRARRIWAPIVLRAIDTVSAVAGPLLRGSTAPSRSIKNVAALDAACGKEQEGGIDDHADMEERKGSKGMMAKAASAMAPLWEAMRSGQAYARDTLTTARQFTQSLVPKDPEREEAEAARAVEAFAGLSAVLSSSERAFDTTWRLAQASMAALTDLTGNLITDVARRYHIPVPKFTWIKGKAAKLAQDTAISSFAKVAGTVPATVVAGMVGVSPPSAASGGKPSVNARGAREGAQAEEILRAEGELADGREGKVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SGW3</accession>
    <name>A0ABQ5SGW3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_013769</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00299">
        <property type="entry name" value="GST_C_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF9">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="31"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="121"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI69197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="29826" checksum="A9B8E5602DCCDDAA" modified="2025-10-08" version="1">MAILRAHSLQTCSGTRRNIISRRTCLRAMAGRMSLYDVPVSNNGARIRYIIRKKGLESEIDIVSPQTIGGLKSPQYLALNPQGKMPLLILEDGTPLPESEVISQYLLDKFASRGESLVADTPEARAKAALATRIHDVYIVPIQGAMYRTMDSAEARAKNIEQIAYQLDVLEGIVEGPYVAGSRVTAADAALFPTFSFFKFILPTYFGWRDVFAGRPKLAAWWSLMEADPVAAKIMEEIHGGLSGWAANNRWRDQGIVDQVANTAYKWAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIE0</accession>
    <name>A0ABQ5SIE0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_013771</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000080">
        <property type="protein sequence ID" value="GLI69199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19494">
        <property type="entry name" value="Elp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008728">
        <property type="entry name" value="Elongator_complex_protein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896">
        <property type="entry name" value="PAX6 NEIGHBOR PROTEIN PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05625">
        <property type="entry name" value="PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI69199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="434" mass="44362" checksum="9392D2DE24E64D9F" modified="2025-10-08" version="1">MSSFRRAGVADTGIRPGLHGQTLISMGLVDLDRLLSGGLPLASVLLILEDAYSGQHLNLMRYFIAEGLACRQRVLWLTGSPPPGGPAAFLPAEASPSQSGGSVAEDDGGKEQPELRIAWQYKKYIKDDSPVQAGGTYRPAPRSSSSSTARTVAAGVGREWCHSYDLTRPMGEEALGASSLDVVRCSGPNAYGEADAAADAFLSSLRTAPEAGPGPGSGAATTATLPGQRSGGVGRLVFESAGSLAWGGGGSGASEQMLLRLLYNVRRRAMQARCAVMMTVPAGLLSPGCAARLPHLADTVLALEGLMDDMPLFKLLPDPNSAVALLSLRKVSSAGMVGPRLPDGQLYVVRNKRRRLALSPVEVDPDAEERQAEAAAKAAEAFGVKAAAAAGGNTASASASAAISGSAGSAGGRKNTAALLCGGPPAAGNKALDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIK3</accession>
    <name>A0ABQ5SIK3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_014108</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF22">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="67"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI69476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="543" mass="57469" checksum="6FF6ED973DF14DAD" modified="2025-10-08" version="1">MADSRAGEVSPPILVRTLSKGRSRGVDFVGQLKVEAREFFLDSKLNILLVAMPLAFISRFTGWSDGATFTLSCLALVPLAERLGFVTEQLAMYTNDTLGGLLNATFGNATEIIISAFAMIQARKQKSSSGTYLRVVQLSLLGSVASNLLLVMGTAFIAGGLKTKAQTFNQTGINVNAGMLVLSCFAVMLPSLLDATQSGARSDNRSELALSRFESIFMFLCYCIYLYFQLKSHSSTRRTSNNGAFTPQLGATTETDLEAATLNPTGTGGGGNGLPLSRTSLDGREVTIGGRYSNRQIEMAETKRTTSGRSVSKVTATDRERDVERLPLVLAATRAGGLEPTHVADDDDGSHRSRPDDAEEEELVLSRTGCFLWLTGVTVFIAFLSEFVTDSIKHAGVHDGGSFLCAAGVDHGLAAASGFQCVRGVCVVCLRLVGGAGDSGWPCKLPQGHPAAADVLFCGSKLLVPQGQAAYDVHVRYTRGSGAHSQSSFQTAVITVNGGDRRGLQLRDSIFQLGDEGLPSSPPALAPAALGAVQLLGCFKAVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIP3</accession>
    <name>A0ABQ5SIP3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000088">
        <property type="protein sequence ID" value="GLI69822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015525">
        <property type="entry name" value="BRCA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289:SF0">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN BRCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="398" mass="40744" checksum="D8200E7670DE3161" modified="2025-10-08" version="1" fragment="single">RAELDRCRGLLRGGPRAAAIFSSACCASFGTLRVQRAYANLVVRSGGGGDSGGGGEDGGGDQDGGGGTLSAEERAQLSRLVLERQAYLAAEVDRLMEDAMKGAAGSASGGGSNGGGGDCGVPYLRLRVVGVTPHGVPSGPPCHAMLRIWRPPEELDEQLREGQVLLATNLAPGAYGSGGGGGKDTIGGPAGPLTDLGPNGRLRCLEITAGKMTRFRILGQAADLSHHLALPGYQPRRQLCLADIAAALPALLQQRLLLPPHLAHPHGHGHQGQPERDVSLFEFDFTGCVVRAGEVQMLSVGGSGGGGGGVRQQWVFLADQTSGLCAAGMPGRPCDGGGGEGSGGGASGFAGLQPWMLAVKMVGTLESVDFLLDPARQSGAVLSFRHLILQDRDVANQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SIV7</accession>
    <name>A0ABQ5SIV7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_014291</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="4"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI69624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="170" mass="18971" checksum="295567FB6FC80F2C" modified="2025-10-08" version="1">MSTVDPTVARLWFESVDADSSGLLDAKELRQALDIGGLDYTLAQAHLFVRAFDSTGNQKLNVNEFVELHLFLEAVQSCFSRVARGGRWLSASDIQQALSFMGHSLSSTAMQAVLSRYDVDRRSHFGVDDFLQMCLFLRTASRAFQAFDPTKCGFAQLNFNQFCYAASYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SJ90</accession>
    <name>A0ABQ5SJ90_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_014099</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000086">
        <property type="protein sequence ID" value="GLI69548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0157">Chromophore</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="13">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="14">
        <location>
            <begin position="81"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="13">
        <location>
            <begin position="201"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="14">
        <location>
            <begin position="275"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="405"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="724"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="564"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="741"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="593"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI69548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="761" mass="82560" checksum="C039EBA351CF3C59" modified="2025-10-08" version="1">MAGVPAPASQLTKVLAGLRHTFVVADATLPDCPLVYASEGFYAMTGYGPDEVLGHNCRFLQGEGTDPKEVQKIREAIKKGEACSVRLLNYRKDGTPFWNLLTVTPIKTPDGKVSKFVGVQVDVTSKTEGKALADNSGVPLLVKYDHRLRENVAKKIVDDVTIAVEKAEGVEPGAASAAATAAGQGKPQGARGAAPKSFPRVALDLATTVERIQQNFVISDPTLPDCPIVFASDAFLDLTGYSREDVLGRNCRFLQGPGTDPLTVDQIREAIRTGTEITVRILNYTKQGRPFWNMFTMAPMRDQDGSVRFFVGVQVDVTAQSASPDKAPVWNKTPSAEDEKAKQGAAAASMISTALMGMAAPATSNPWVSIGGEIMRRKPHKGEDKAYQALMALQQRDGKLKLMHFRRVKQLGAGDVGLVDLVQLQGTDFKFAMKTLDKFEMQERNKVPRVLTECSILAAVDHPFLATLYCAIQTDTHLHFVMEYCDGGELYGLLNSQPKKRLKEDHVRFYAAEVLLALQYLHLLGYVYRDLKPENILLHHTGHVLLTDFDLSYSKGITTPRIERMAAPESGSGSGSGAAPGSSSGSKSRKSSSSKSASPAPGVGPTGESYLLLAEPVARANSFVGTEEYLAPEVINAAGHGPGVDWWSLGILIYELLYGTTPFRGSRRDETFDNIIKSPLRFPSKPLVSDEGRDLIEKLLIKDVGRRLGSRTGANEIKTHPWFKGINWALLRNEPPPYVPRRASKATGSGGGGSAAAFDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SKD7</accession>
    <name>A0ABQ5SKD7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_014511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000089">
        <property type="protein sequence ID" value="GLI69832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015525">
        <property type="entry name" value="BRCA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289:SF0">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN BRCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI69832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="146" mass="14488" checksum="BCE54556EA437B07" modified="2025-10-08" version="1" fragment="single">GEDGGGDQDGGGGTLSAEERAQLSRLVLERQAYLAAEVDRLMEDAMKGAAGSASGGGSNGGGGDCGVPYLRLRVVGVTPHGVPSGPPCHAMLRIWRPPEELDEQLREGQVLLATNLAPGAYGSGGGGGKDTIGGPAGPLTDLGPNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SLI9</accession>
    <name>A0ABQ5SLI9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_015402</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000094">
        <property type="protein sequence ID" value="GLI70496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31086">
        <property type="entry name" value="ALUMINUM-ACTIVATED MALATE TRANSPORTER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1225"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1279"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1305"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1360"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="265"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="476"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="615"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="856"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1028"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1119"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1497"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1688"/>
            <end position="1744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="329"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1038"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1049"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1127"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1506"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1692"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI70496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1836" mass="193660" checksum="0960EED629C23704" modified="2025-10-08" version="1" fragment="single">MFKLALVQDNLQRWSRPLVECARNFIISPPFQAAVQFSVGTFLAGLFVFIRPISFNLSCLASLLVVVVVAVLSPDNHIGTRLTGAATLLGAMAWGLVLGGAVLTLAHVAVQSTCTTPVGCSAHTAMLCCLSVVLIAVLSVNRAAIGPPYPPHVCVAGMVSVLAFGLVLIAGQFITNMRLLWTNVVGGLLLSCLVASGFSSLVSLLVLPSLAVDEMRADAAAAIRGIGYAASRFASRGLQQEQSGAPARTRPTSRLQPHVSIINANTSGLTGADQRPDPSVSSAIAAGSEAAFEERHSKPAVAGSIPIWKDPRVRQESVAAGGDDGAEAGFERDSRASESGDDHDTYCNCCSCRNDHSSSELPQAPSCAAALDAGASPVKLSLGRFRHRARLSASPGCKAAAPRDLSPTSAAAISPMLPAAPCGSRPSAACAVHRLGPSDSAALAVPCDAAPGSSDSANAVAEHAVEGGSVHFGYGQGADYRPPSAPTSEDERPAWPLHSMPRPRIGGGGGRRYVGLGGGDGSDFEHVLEDEDEFLRLLQEATAPPVTYSTMTVSRGGRPRYARAPPLPAVAPPGGILPSPSTRDLMGLGGAASIAVAISAPSAVPGVQASGRAQAVKAQKEAGTRPGSAEASAESVGQPSSAAAATHGSGGGAMKSSTVAWTPVSALRPLLARARACAAAAAVEPTWMMRGPANLTAWSGVLSACETLLGRVASLECLLEDHHPGQPGHSLGDVRLAAVLGVDVVSPYRLVYGEVAASCAAMSVAVANSIQGHTDKSFTAVGGWQLSKERLSSLVSTTLSSYWSRVRAAQRHHCYCRYNHRHHHNHRGDVDIDVPVVKAEHGIIYDRAAARASERPLSPIPPALSSASPLKGTGQLGHLRDDSSHPPQHRGPLNLHQVREKLSPRSGPHHQGAHLPLHQQNQLQQHMNELCCQLHYGSLRHQPQLHPHSHHHVGMPLRAPKIRTVSLHQARAMTFLWTVTEGVISAMDELETAVQQALSKPRLTGKSSVSAAAVDIQNTPCALKSTAALMPAQPPKPTSLSSPPKESMATDTAPKSGSEASETGCSCSFADGNGEGSRGGDGDAIVNSAIHAGGRASSEVIDANAGSSEGVVLLVGEHTAPSQASKEQQRHLKPQDRPENLGSDLARPHGWWCLAGLWRSLTCSHSDSGDEWWRFGWARPLVEITLGIPMVRHCALLVVSHFRTLLLSGAGPRSARLRQRLFQYLFKYWLVSVSTLVFILGLAARPDAGFMRRYPMLYAYIATSVSMTDRVESTMSRSALRAGGTLLGGVLGLWVMLLHKWADSPVGLMAIVIPVVFLTGSMASHPLLYGMYLTVGTLLSIVFCQYGTSPYCCTTPGGTLEMFAARVVSVLLGCTLPVLFSQAVLPWFTSDWALETMATAFINAAIIVRQLYRRFFDEHLRAHMASWGALATAELLQLYGMEEEAEALLGEASASPANTAPAESVEVPVQICHTGREQPDLELQLRPQQQSLPRLQQQLVGDRDGSGASSSSSSSSSRRRRGGANGGKSEGLLEAGREPGAVVTQKAATQVTALEVMTAAARAPSPPSAGDTALATALRTLVSGPLVAVQTSLMKDTTVWSRGIYAVPLIVSAMLRSGLQLADRLAALQLVVADTPPPVHGQLSGWAFERMVLPLHKEMMAVIAALDCLSAVTARLLDRMASSAERLSGAACDDRVSDGDRDDGTGGGGSPPQPRCKSQEWRRASGAAGNESSPQRTRDGSRTACGQKLASFDVEGGGEMDLRDCPRAAEVRAAVQELHLCRLQIRQHIHDMRRAFHADVRSLDEQRLSYATHPDDTVHVNSMLYALVKVLDKATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMB0</accession>
    <name>A0ABQ5SMB0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_016220</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NIPA (TC 2.A.7) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF9">
        <property type="entry name" value="MAGNESIUM TRANSPORTER NIPA8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="331"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="423"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="595"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="700"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="942"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="992"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1092"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="336"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="463"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="479"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="617"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="645"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="706"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="753"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="826"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1099"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1114" mass="116912" checksum="B31696985E95337D" modified="2025-10-08" version="1" fragment="single">MVESASPLPPFPSQSPSPSPAAPGKTTGGLWYVGAIINVVGSVSINLGMNLMKLGHTKRAQLDMPEEQKPPIRKFKTWLIGIIIFITGNILNFVSFAFAAQSLLAALGSLQLVCNVFFAYFVNHEAVTKLIILSTCCIIGGCVLLVVFGNQSSETYTVDQLIHFYTLPAYVVYLVMMAVFVFGFYVLYLHSKKVCRKRGERGLWYGFMLVSYAIFSALLGSQSVLFGKSISVILRTTFTESNQLGNWYTWVVLPLFTFTALFWITRLNKGLRMFPAMIIVPLLQIAWTLFSIVSGMLYFQEYKGFTTLKAIMFPVGVVIVFMGVFILTSGGRAPPEEEEAKEEVGETPEDGQRPPKVSMYDNAMYSARSDMDGHPGDMPYGPHTQSKMSLGQTLRFTSTWAPPSVALSPGSNLNRTLVASDTLRRQPRLTGPSDLNATDITSRTDDLTPTTSVAGEQQHHPGDGGGGGQGGWGNRTYDRNSTLNRTAQSTVSRISETSMVSTMRKGLKTVGSALTSDMGLNMAGFKLAVGLGDSHLAGVSLFTMPALDFTATTRRKTERSEYRSSRIGTRNTEISILSALPRSRFYKGDPDNLTVIREASGAPPSSVPHSPEVGPSPTDSSQLPASSLDSNNAAAGATAAMGRVASTTNTQISDVGPSTSTSQPREPPPALSGRVAPDQPLTEAAMMDLLPVPSFQDARSAPLATSPAEGGEQPQQQQQPSGSGSASEAPGGPPPGRPGQGTQAAGPPVWTTGGIGSGGSRPGSGQRAQRSRRPSALPESGRPTPPPSAAGLGGLAGAASALHPGYDSDAYPSARASPPGLQRYRSGGGGDVGSGGIGGAASYDGPPAAVMVGPGGLRAPRASLPKPGSFGAILPEGVPLSTANSVVIHESEEATPTEGLVGIGGIGMSAGMAQQHQRLLPRQYGQHGGELWPMYGSVTQGGGIVQQPQSQPQSRAQLQTGWRYSRASQLPQTQQNSCLLQQASLLHQQLQPLPQQQSVQSSHQQHQSPQQIPLSLIHRATAAPAGTVRLPALHLMVPGLPVSPLQQQTALAAGSTGGTGTPYMQYGPSSPSYAQMLTGQGVSTEPGALVLQQTAMSGPVGGSSAPGGSGPAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMP9</accession>
    <name>A0ABQ5SMP9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleoside phosphorylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_016385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17769">
        <property type="entry name" value="NP_TgUP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691:SF14">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="2">
        <location>
            <begin position="94"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI71265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="403" mass="43300" checksum="FB7ADC104B7E3D42" modified="2025-10-08" version="1">MYLSGRRRAMGPKSPCSDMLGRQIRVNVVAPPSLSAYRRPRLVTRAQVDKADDHAVAVIGRHKPTYKNANFPTDGEGRTYHLGTKEGEVAPRILSVGSVSRALLLSKLLDPPTSGRPLFQRTSSRGFLTITGRYEGEPVSIVSTHMGQPNMDFVVRENRAIVRGQMAIVRLGTCGAVQRPAKLGDLLIAAHGSIAIRRDPDYWTLMDGRESLFRASNGASRNGTANGTASTNGSGGAATSGGFTGRKPYMTSLPVPANSQLAALLAAEAAQVVGPDRVVEGLNASADSFYSSQGRTGSDFDDRNECLLADLVAAHPDLVSLEMETFQLLDLARCTKGSIKAIGMCVALAERYSNAFLEYHKLEELEISGGTAALRALVHVSLDGPSEAEAETLKEREVQYVWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SMV1</accession>
    <name>A0ABQ5SMV1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_016120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI71004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI71004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="156" mass="16520" checksum="3B8FA85BB3213E8E" modified="2025-10-08" version="1" fragment="single">MDEDGHYHQGFAAAAAAETAGLDLSSAEAREVIVATVRTREGADGDSSSLASRPHDRNTPSATGLMNSLLDGSSTPAVDCMQKQHLDDGAYHHIINNRDHSDGQQQQPVAVADAAAMTTAGARSVERSWPTAADYCNQQDDAERQGRHHHQGSAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN03</accession>
    <name>A0ABQ5SN03_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_016479</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000103">
        <property type="protein sequence ID" value="GLI71320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="178"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="195"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI71320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="224" mass="24607" checksum="BC714C5B4FB66377" modified="2025-10-08" version="1">MGNLYSKKSKAPTGPAITDVDRAILSLKTQRKKLEDQEKLLNMRLGRHAEVASQLTKEGRRDRALLVLKKKKLSEKQLVQLHGLIINVAEMLSNIEMMKKQTAVFAALQSSTAALKQLQAQVKLEDVQRLLDDTTEAKQYQEELAALLGQQLSDVDNSEVEAELAALEEVVADEEKLEMPRVPTTKFTEAEKATAAETTAATAGTPGKAEAAVEEPRREAMLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN41</accession>
    <name>A0ABQ5SN41_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_015838</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000101">
        <property type="protein sequence ID" value="GLI70843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="428"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="601"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="869"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="931"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1025"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1148"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="759"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="601"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="630"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="949"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="968"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI70843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1233" mass="121195" checksum="0068A7D6026E2075" modified="2025-10-08" version="1" fragment="single">MALAVEVVSFQRTMGEYNATSTNPSIDVNTIIRNLKLRRESCTNIAAFGLASPDYDDNLPATTRTIPPRPLTRPDPGSVTAFGKPVAAFSSGANVANEASEEACTDATDTVMGRRYRQYSAYTNPTSPPATGASGHHSNENHLSDFSCGAGNPTCGKTVSTVPSLPPDGRARSLSASLPASGIPSAVYPPPPPLGTSFLSSPSSLPLPPPPPLNGFYGIAHEDSRFSAAPTHVDATAEVTMLIPSASAPAPSHLVAALSPLGSSKSPAISLPLKAISSSVLLVGDNAASTTYYPPHLSKAAVNARSYGNMEGSGKAAAALHTPVWGQSSTWQVTDVVGSDGKGNLAGDDRGSASGGGGAGLESGGGSSCSGVGGSHCASPSLRLGSVAATAFAAASAIVAATSAAATSGGRSAAAVAVAAAEAARDVADAVPGRQSPPIPSRSPKASCPPGEPAPPPPGVLVTAVSSPSRSGPQPQGGPSMSGDSGLASQRRISLEQQISRLKGSLTANVNGAVTTTNGQVNGYIDCYGTGAAAANEGALANEPANLGRTVSGAVSLATAATVSVGAAAVTCEDDMLTEAVSAAAAAAAAPRYTKPVNIGPSYQNTFGQQSPRQNCRTPPLSCLERTDGSSGGGGNGKSSSSGGGTAVSGPLSPGSYRGVVLPPSASLPVPPSYSGSGTIAALPASVAAGSTSSRATQRPQLLDELHSAHKRELALAEQLGRARAEVLELSSQLTRQAEAAAAAATAAAAEAATALELRSLLRKERADADEEIRRWRSRAAAAEEQLQTALTQLACLRSAAAAAAAAGQWSPAAAGPAWHSAGGRMAAAATAVVAADQCDQKSPRSSGTMLAATAPPALAAESVVYHDEKGDEEGVLSERTHLPSSPAGPPSPPRRSSRSSPSELDATLLRRSLSYQALCLESGIEVAKPPRAVSTSGGAPGEEGDGCSDALDTAAAGAPGDGDSSSGDGGGGGGVPLGSPPILASAAATVAAAASFSSGLVGGGSTRPLAAAAAATQSPSCRASTAAASDGRDRSVASTSVTDNGTVSYEPLSIQAGKVHGIVYGVSGDDGYAACTESADGGGGWGNWAVAANAAAAGGSGVAEMTQVQMSYSVPPLPSNMVAPAMADDASGEWGVRLEPAASATVPGTDAIGAGRGSSKTRAVVGGQPASKGALPADIAALAAVPEGADGTAVLQSSSAQPAGLAAHGSPGGNGDAASAVLLMAEVERLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SN80</accession>
    <name>A0ABQ5SN80_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_016687</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-prolyl-[collagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:18945"/>
            <dbReference type="Rhea" id="RHEA-COMP:11676"/>
            <dbReference type="Rhea" id="RHEA-COMP:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000112">
        <property type="protein sequence ID" value="GLI71463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF238">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045041028" description="Fe2OG dioxygenase domain-containing protein" evidence="8">
        <location>
            <begin position="26"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="9">
        <location>
            <begin position="136"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI71463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="271" mass="30846" checksum="6EC469CD4B6CE835" modified="2025-10-08" version="1">MIWTWHLLILFALLCSLQINRHAAAFEEEQLIGWKGETYNVKQISVSSPQSTGPWIETVSWVPRAFIYHHFLTPEECDHLIEIAKPKLERSMVVGQEKDLVDDIRTSYSASIGYGETKIVAEIEERIARWTHLPIRHQEPMEVLRYINGQKYDAHWDWFDEDEVVKAGGSNRMATVLMYLSDVDPKAGGETALPLAIPLDSNTQSVDGRGYSACAGKMGISIRPRKGDVLLFWDMDPAGHVPDRHALHASCPTFFGTKWTATKWIHNTPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SNY1</accession>
    <name>A0ABQ5SNY1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_017003</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000117">
        <property type="protein sequence ID" value="GLI71697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13156">
        <property type="entry name" value="KOW_RPL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000915">
        <property type="entry name" value="60S_ribosomal_eL6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041997">
        <property type="entry name" value="Ribosomal_eL6_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01159">
        <property type="entry name" value="Ribosomal_L6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI71697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="24669" checksum="EC1E4BA815627BA7" modified="2025-10-08" version="1">MAKPAKFIARGITAEGRAKTYKRKGLWAIKKKNGGKFPTHPKKVVPARAEGRAPRFYPADDVPKPLAHKAVHKPTQLRASITPGTVLILLAGRFKGKRVIFLSQLPSGLLLVTGPFKLNGVPARRVNAAYVIATSTKVALPKLDLTPFTDKYFQGEEEKRSKKGESEFFTAADAKKKELSQKYIENQKTLDAAILPALSTDLKGYLSTRFTLKDGDRPHLMKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SQE5</accession>
    <name>A0ABQ5SQE5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_016866</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000116">
        <property type="protein sequence ID" value="GLI71602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI71602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="120" mass="13574" checksum="DE970635D77234DD" modified="2025-10-08" version="1">MLTYVGRGWQLRLRLATRFDVAKRVCSTINETMKVDCDGDSDKAALEERKRRSIQTYLDHQLRASDPDYGTLAMLLQRHGVVVRGGKAEALQLMEALAAWKRGEWTTPEEKPYNASGSCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLR3</accession>
    <name>A0ABQ5RLR3_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">VaNZ11_000304</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18041">
        <property type="entry name" value="DEXXQc_DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026851">
        <property type="entry name" value="Dna2/JHS1_DEXXQ-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014808">
        <property type="entry name" value="DNA_replication_fac_Dna2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF433">
        <property type="entry name" value="DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08696">
        <property type="entry name" value="Dna2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0347">Helicase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication factor Dna2 N-terminal" evidence="22">
        <location>
            <begin position="687"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="23">
        <location>
            <begin position="1425"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="23">
        <location>
            <begin position="1527"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="24">
        <location>
            <begin position="1603"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="150"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="574"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="607"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="958"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="GLI58557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1885" mass="199408" checksum="4C370751738B9347" modified="2025-10-08" version="1">MGKKHPAGAAKQRPGITRFLERRATTNEAGTVTDGRFTAVLRSADGRASTPGKQLLDNVLLSQDADDGGMQVVWASSPTAAFSPQKAAGGSQEGSISVSQQQAENSQASRDGFRKTLIQAVQRAPSDADLDVGVQLQQPIDDMMLEPLSTRRSSHRSASTSSPIARTVAPADPKPRGGFAEGPDADAMPISDYMVMQEVLASTDTDRTPAAGGRPCRAVVAQSGDAIRPVTCTPSVLTVGGKRPLRASGKKAASGGNKRRALLDILDQVEFMVKHGSSAGPSQPSAAAQRYLRIEADMLPTASTEEMKYQACPDAETPRPADAEAVAGNASHATPAARCAPGAQASRPALNRWEAALLNSETLDEGYISACGVAAPELTTGSLQKLPMTADYVMPTQPIRNELPDFNSGRNCNTSDHISKGRVAAVAVNGNVMPDRCFAMPPPVAPMAARALMSGSGLATQPLIPEQVLATPIQDVASLVRGNHPPAGGPAAAMPQTVAVRERCQPSRAATTGKCTQVVCPEVCGEVSREVPVSLAAVDDDDDAFWGDVDVDALVANATSWQPNTTGAELIGSCPTGINPAFPPEPSHQAAPGIRPDEQWNAHRLQDRRNSPPKSEMPVQQQQTESGPPCGQYKGALSARTPVRSEGGSSPAVPLLGEREEVHFVVLEVEELPSGHGKVLRCFNEYKGVETRVCLRDMWADQEVEPGDTVNVVGGRVLADVTCKFLEVSATEGLLILHPDVLLSGTTVCTAIRCARQAWVQERVASGPGGEKALIGSMLHELLQRSLRAAIAGKLDKNVMITEARSILATNTDKMLESQQDEASVLAYIMQQLDAIISWCNSYITPNPSAPLGGRIEVGSGGTPTFCRVAEVLDIEESIWAPKYGLKGQLDASLRVKLSTGEPLQVSVVQNCSQQHPAHFHGLRGRRTSEGAQTGAVPTGDGVTGVIGAGRRQPDMGCTTGKPQHLWSASKAASGAVPSAEPHGHRPVEASLDAGGGVIAPFEFKTGKDRHEHRAQVLLYLLLMEDRYQTAVQMGLLWNSQKPTMQAVRYAHHELASLIMHRNRLAAYLVGAAREPPAPLQGSEADRKCEYCGVSSTCALYLAAAGTSGAVQSDAPLVAPQSADMDLHQSLREKLLTVSPSSAAFFAHWVGLVDLEELSVRGSRTDMWALSGAQREQLGGCVSQLLLERELPPNGEAENQRFVYIFRRRTDTNEAASGPGSAEASLDGGADAEIGSGGFLGSGFVPGDSALLGVEGRHAAAARVTVVEVTKDTLTLSCRKELKYIKLGDAPRAMCGRCTNGPHPPAALGCCNACAGTSERVTWRLDRDESASLFAQLRLNLALLCVAGAGSGWSGGCGHKVGAGDAARARRESHLAQLRKLIIGLEPPQQGLGRSSLCASSQLSASAASTQLQDNSYLREHAHKMNGEQLEAVQRVLAMQDYAILLGMPGTGKTTTIVHIIRALVETRATILVASYTNSAVDNILMKLIGTPVSFVRLGSAQAVHPAIRPYMPGGEYHPDTSVAGLQEVMGRVNVVGCTCLSVHHPLLAGRTFGVCILDEASQVTLPASIGSLALARSFLLVGDHYQLSPLVVSREAAQGGLGISLFRRLSEAHPQAVVTLRSQYRMAADIMALSNALVYNGRMLCGSKTIAEATLRLPYLTNLGSHGAGAAQESSPCSPWPCDVPVPTWLMALMKPDCRVVFLDTDSVVGCREMVLRDGIINKGEVHLVYAITSALITAGLPPSDIGIASPYKAQVVALQQALAGLGVSSLIEALDESTDSGGAVEVLTVDKYQGRDKSAILLSLVRCNTSHRAGRLLTDWQRLNVAITRARTKLVLLGSAATLASIPLLQDMLLFLKCKGWIQLLPVDCLAQLPVLPTVKSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLV1</accession>
    <name>A0ABQ5RLV1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">sucrose-phosphate synthase</fullName>
            <ecNumber evidence="2">2.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000195</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>beta-D-fructose 6-phosphate + UDP-alpha-D-glucose = sucrose 6(F)-phosphate + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:57723"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.14" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044161">
        <property type="entry name" value="SPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039">
        <property type="entry name" value="SUCROSE-PHOSPHATE SYNTHASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039:SF5">
        <property type="entry name" value="SUCROSE-PHOSPHATE SYNTHASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="7">
        <location>
            <begin position="90"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="295"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI58585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="786" mass="81352" checksum="78E158E6E45956A4" modified="2025-10-08" version="1" fragment="single">SHQPRSPRRSFGGGGNPPSTPFASPPVFIRRGLAEGEMSGRETGAAAASPSGGALGGGSLPLQAAASPVTQPQPSVFTEPPIWREVHRFLRNPAKPVILAMSRPDAKKNVATLIKAYGSSAVLRDLANLVLVLGNRDAIDSMASGSARVMEGVLKLVDAYDLYGSVAYPKRHSQSDVSDIYHFAAATRGVFVNVALQEPFGLTLIEAAAHGVPIVATCHGGPTDIVATLRNGVTAEPGDVGAIATAITDIITRPELWDTFSNNGRNNILAYSWPSHVLSYLRVVEARRAAEYPTAAAATRSGGSPGPVGMAGQAGGGAAAGHAAATPGGGGGPPGCHGSPSYHLHLRTSYSLSYDDLSQLSQAACYQGTRLSLAGRSGGRWVVFCCDCDKELAQAGGALAALSAARRAAAEHSLPLTEAADRLGDIRLSALLRHALADVRMDAPAACLGLGVLSCLGCTDTRALLAAGGVALSELDFVSCNNGCQLWLTGIKVHRNGVGNSANSGDSGGGGGGGENVNGNLQLEDPKLLFDDRYERHMEYRWEATTAKQVVRRILSNRSGWGKLPLGQPGRPAPRISALGPYHIMVTFSGAAARDVNPTVLLTRFRRKLRLSGLRAQVVITPAVASSSCGRATSGCAGDGGGGGSRGAGELRANSMGLPGAVTATDSSPSLEGFTLHVVPLRCSRAMVLRYISAWYGIPLEDVTVVAFSTGGGHIATAALHCSDAEDLVAGQQRVVLMPEAPAVVEVAAGGCRRQRHHQQAEEAPPGIFTVDLSPYLTSGRVAVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RLX9</accession>
    <name>A0ABQ5RLX9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_000190</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF214">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="660"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="587"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI58484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1158" mass="124684" checksum="9E1D01F4795EBA03" modified="2025-10-08" version="1">MDSSAVKPRRHPLREKHLGPVIIQLHFITLAALSLRDANAASATTITSERKVYNGLTFARAVADASISTLILMEDVVLQDGDWADIPMPLLLVRNLTVTSDPSELNNPRILNMNWVAEKIRLGNKVLLRLQSLVVLNYRLGNHNQAPGLDILSPCSPGTSAVVEVVDTVGVHRVCYPPRIQEMSLASVKRPASIPGNQSYHSYLAQSGCINDTSARPLERCWPHRGLYDDVAMLGADPKDYNLVQLNGYIVHLIRVVYLCELMVSEDCVANMTALGCATFTYATLNPPWRPLAQPRSPPTSQIEVAAAVVPAVQKRTAVEPLQPAAAERLGGLSTAQKGAITGSVVGGLGVMSLAVLLALLHRRRMRQAAGEELPSTLGSQWRGLALWECIRLSVPDSAAIRRHFGVVQIPASSVTSSTQSADAVEAGPAAEVTANASRAKGKAVLEAFSAGAASISLGSPFGPSQRQASDTGSTRETPTAAAIDGLGLAQGPQRAAAVIEATGAAPATAALATHGGCSVTDDGDGSSEATGVTRLGGARDSRPAGWMAIAAAAATMASGDSVRGVLVTPLTPHRPDVNLNLVPGRQQQQQQQQHHLPQQQQHSYEEEVNVKIREHFTTEQAAAHTVRDTGTEGAQPSAANTFTSPTMGPPCTAATAEVVLLPTVRGRGSFGRVVEGMYQGQKVAVKLMTEGLAPVSMSNERLLRSFTQEVEVLARCFHPNVIRLLAACVAPPSLCLVMELMDTSLEQLIQQAPGRLLPMSTVLRIAVDVARGLEYLHPTIVHRDLKPANVLVNDPLGPNQVAKLSDFGLARLHCSELMTTNPEAGTPAYMAPECFDVTVASITFHVDMYALGILLWVMLTGIQPWEGVNFMRLAFKVTYGGERPPLSAIPPDRRPHKLLRLLRSCWEADPRRRPAAAEAVKELMLVQQMMEVASLQNTTPLAPNLLQQPVQSVPSRQPQLRCMAVFGGLTSCAAPQAAGVGAAAAEHQQPYSPRDGHQHSQICEQWTLHEQRLCEQGLQGQRLHNQRLYEQVLLQLLAVGRVPRSLLLQRQSSFCSVHSRWQQQQLLLKAAGSHEAAWELCTEDFMQDCRKSHNGLQQQQQQRQKQHSCASAGRLVASLDSGWRTALSARLLEELGGSWAAAETGGTAVHLTIDTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RM35</accession>
    <name>A0ABQ5RM35_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF157">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 12 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI58653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="461" mass="49181" checksum="CE5F903FD5860959" modified="2025-10-08" version="1">MTGINNAMHASDLTDIDAAPVATVDGHQILISVVINGHRITISKRNLEQFDAQARAFAPRNAFLVTGQNLYAGFNTHSQELFSCHFQVPCICIRVAGIHFTCCSYSGIPVRLSNALSCPSPPQDPYFFDSDYTIAGSTGFLIWEANWLVLRLLRSTVSPSEMQRTGPLNPPPLLVPARHKPLFHLHHLLAGHRVLDLGSGTGLAGLSAAACGAHVLLTDLASVCEGTLRPNVQRNALPAAPGKASLINAALATSINTKTSGSVSTPVGTCMAFGDASADGAQCEDETGQQARRETTPGWFSGSWAGAVGVGQGSAAVMALDWTEPLEPQIWAGGNDPREADFILAVDTIWLLDIFHSFLDIVYAVLSHNPATGAAATTVIRPTPGVQPAGNERTNPVRRKRACFLAFVDRAGADSKLFVRKEAVEQELRNRGLDVDIVLAEDVDVDGVARPGRVLRVTMPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RMJ9</accession>
    <name>A0ABQ5RMJ9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_000558</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI58797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027912">
        <property type="entry name" value="CFAP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33487">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33487:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14858">
        <property type="entry name" value="CFAP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="833"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1311"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1463"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1586"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1693"/>
            <end position="1721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1842"/>
            <end position="1880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2240"/>
            <end position="2266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2320"/>
            <end position="2391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1316"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1326"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1483"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1614"/>
            <end position="1629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1696"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1870"/>
            <end position="1880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2334"/>
            <end position="2346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2373"/>
            <end position="2386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI58797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="3113" mass="330627" checksum="297D69B120773AF6" modified="2025-10-08" version="1">MASEVIALCQSFEQELSKTLAILPPVSASKPDLHDAHLNHHRLSQRLAESVSYYAGRLPAYASVPRILAFGDRLFRLEQHQLALQACYKHVRSLDLNTSQENLPRMDELTRLSSHVQACFGCAACEAALLLAADPQIKHPDTLQWLEQCLSQLRTAIALALPQERLYWLVANGTVHVYTTAKAMITAGFAEQALPALIFCIKALEGHVCFAASKYLPWRTQLYTWAVYGLLDCRAIEQGRMLLADGLKHIEQLVALQKLDPVPAAPHVQAALRVARFTLVALQLKLEVAAGAPVAPVLEQLSAAAAAVAAAPGGGATAAAAIAQRTLAVMVEALHVPYRRIVRSEPADAPLKELFDATLVVAAPLSDGLRKALERAGAETATADTGVGERTPPQLSPETAASLQAAHESFPYNIHKGLMCAAYNLEQWEQFGQLAELAAVRTEGAVTATGSGQSADAHQSALQLATAGSLLQALRRLTTEPGVGALRQLAAAIQQSLTRGVPEAVSSSGAGGTRQLSASGGGAVNNGGGPTAPSGTAKSAASAPPHQSQIAPWRQLRDLLTDASLALFSSARPLIDGVFSTEDKEAATVSELLAACHAAWSTVELDDGELRVSVALKLALLLEQNGQLMPAKEVLLQAKLIAQRTRVELMAANRRGPEEHVRWITASRSQPSDETAAMLAGMTSSEQELACLHVDVLALLARCELGLGLAEQLGRANTRRTRVLEEQAARTAQSSIFGARNATEVARDEARLAAASSTTPNPQVKERELLQACGKNPYERALLLIQMTAFAGNDSVRKAQLLQEAGDLLAKAQTAEDALFTAAQPDLRVRRDVPPQPKVLQRTPTSVTLVANPPGPGLKAPVGPGRKPPTCYIAYCKSYGAGVGLSMNKTASEYPGSGIMVPLGQSVTIQGLRTNDTYLFAVALYDDAGNLVGGLGTATPEVLLALPLPLYMCWAHLLAAAVRCGAWASAKRAAGVLLPHFVVTTADPPLWRANPMDAQRLHRVHVAAAARPLLRAVIQSIYMYAGAALRRTATMPATLTMPAAPVPQVALPGTLPQLRTPFLEEQIARLKAARLLVMGMEVAALLPDEPLMQEGALRAYQLLAPLLALRAPRTPLLHKALAACHLVLSSLTNLVQDSLHRQEHQRALAARVTAAVSYQLLRLSDAADEPAAAAHFGRLKVELLKAYDPRFVITGRPPPPLALQDEASRLHDVVLQHPRLQEWAPEPVLERQKDGLDLVAKVLPILATASPMDAWASALGFETATEHPRWVELVVRMIEAAVRKGNPVNAAMVAEQLRWWVKARSKIPSLSALNDPNAPQPGASPAAPPAPAAPPATPPPPPPPKWSLEEAAAALTPAQIAFVAPPLPPTDGLGPEEAAAALARYAEEVRSLEAVQRRRVAAVLLLQKRLPKLLVRKRAIETIRENLKRWAPWMSRLNTIMGLLAAAEARKAVVAAAGLRGSPSSAMVAEPSTLGEEQSTFNTAAATPPQPVAATPLPVMPDAGGTVGPDGRPAEPQPAGSGLPPVLDAMIYWARAAQLAARGGAWLELANAVRHAWNLAQGVLNHDPSATVPVPRVVWERGLLPVPPQPPDSMLVPATPPVVDKKDKKGKKGDKKEEKKEEKKEEKKATPKAGNRKKNQVVEEAPPPPPRTFPLAVGSRPNMGRAVRALTEALMELVDRLRDGLHLHTWVGPVTKGREGQDNDVSKSGAEEDRPATAAVSDSQFSYGSDLQVDIWFKEGPLDLAWVSRFMGAVAVVLSRCDRWHTLVDVGRTWARLSEGAFNERLMPWLLQAAPRTGTDPGPFQAALDTFVRDKNQALDQLDKVRMLVRERLAETSLMAQSMGHKVRKKRKPAVGPRSTGAGGGLDGRPSTTGTGSLVGASSSMNLDNMSMASAAYTYRTASTFKTKAGQPDFLRIPAEYEKVIEVLKRRNEKGFMVLAMHELGDVHAHFGNWGGAATAWNDTLDTLLGPYQAIKNWRGKLESMQPGATLQAYGLHGLLLGAVLTGKLARYTYHDNLHIRLEACRLAARLIFCAFSAHVGHPQRLLGFAMYTPRELWAAGADPWVLWNDPYRCPVVDLVGALETVTVCLLDNKLPLEALPVIALWEHVAKDVTRNLHATALSRIVRCRALVALGLLAEGISVASTLMCASNLPDPTLDSNYVLKEPSGAVVQVDPAPPFTNAMLPGHPANKAALIYIADTQLHASVERLYGSWLVAHLTLVRAQLLMLAGAVPNQWRSTNWKTGERVEAPKPTTPPAPKGGAKGGGAAAAAAAAAAAAAAATAASEAVGAGLPEVVEPMILERAYQLLKKALVMARGEDQPAAAVPPPPAAPTTGNKSGSKSPQRAVSPPKAGARGRSKSPITSAKGGQTPATPPPADAGPPAPPAATAGQRGEVMVRSLLLQCELEQLRWHPARGLVHALEAARFIGTEADNINSPQQGDNDELERYALLPVLWFLARAAAVSCAAQLGQVEHVHSLIETASARDAAVVQEQLHVVGMAHIQALVLAQEGRMVEALAALDTVVTRYRALVVYDTRLASALLDAAALRDRMGLRADAARLAAQGLEVAESYAMQLGLGEALIAPELTNVYVDGTALYAHALGVAVGHASRQRRHAEAEKHAVQALRLLRQHTRGLPAQHAEAALAVGRAARMLALCGEGVGYDDGTTTTRPAGVAATAAKLSAARTALTASIILASLDGGHLRDLMRSALLELASIYIAGMDTHAAAACLRAAHSSSAKQDLILLSSHQLAPVATAQLPDWAVTYVKGQEARFSAEAGRNPDAPIADADLARMVVCLLGGLIRSVNSLPISMGFRSRAQAKLAALHSALRFTCAKYGMDVCFMDPPLPPPPPQAAVAAPAVQAAATPAQTKVGASKAATPVTASKTATTAAPPPATPQEVVEVPIVDGTVLVQWHCQEGCWQEPRSWLADGSLPGSGTTIAAPTEAQLLALPPVESYASLLYVIAAPRNDGGTSGPQCGEVTFSVRDVRELQRRVKQLRSRLERPKAPTDILGYAAPSVGELAELLRAAERLLSAIPRTSDDGSSSVNMIGPMDSTLSSGSMAEPPLVGAVRPNLDLSFLSRLEAFLNLDSGIEVVDATLGSWLLQTLPATM</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNG0</accession>
    <name>A0ABQ5RNG0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_000785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI59095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20306" checksum="BE56890CD322E977" modified="2025-10-08" version="1" fragment="single">MGKWKGDSLKDIKDYIRWSRRAKLHLKEEKLWSENTDRPISAGDKAEDAKTALCTMVGDDFLKEIEEATTAAAGWDLLKTMFVKQMSLQQQEYNAKLSTIKKRDTESIDDYLRRASEYVEILKATSFNVPDGIIVSNIMMGLPPAYDMTCAILSAAAKFHEATVKTIAPALKATELKILGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RNX4</accession>
    <name>A0ABQ5RNX4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cysteine protease</fullName>
            <ecNumber evidence="13">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">VaNZ11_000826</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NIES-4468</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. In addition to the protease activity, also mediates delipidation of PE-conjugated ATG8 proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with ATG8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase C54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="13"/>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="15">
        <location>
            <begin position="74"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="15">
        <location>
            <begin position="472"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="275"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="341"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="637"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="GLI59003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="664" mass="68905" checksum="2E78E6E8475BE677" modified="2025-10-08" version="1">MSNGTGTATLTAGQSFYLRITQFAFSMSQALSLRKLADIMHGTTNLSQQPVWLLGTYYGRNKGSNKVLDPESLEAMLADFRSRLWLTYRKDFPSLGPSMLTSDVGWGCTIRSGQMLLAEALARITLGRCWSRQSSSLDEAQPVVKLFLDHPDVLLSIHRICTAGVSAGIVPGRWVGPWMLCKGLEALFSPLGRERPMGLHLHVACGSGGGAPELDTIAIRAQMAAAAVTTEDQGTSKQEERVSIHGRCKVDTAPACGGDSAPTLCSGSAKVGDDIDTASGGGEGGSLQAAVGSEGWDDPDRRSSDGPLQAADAAGTDIAADRDLQSQPQLRSDELLGRVMGPLRSGKKSQGASALETTKGPSEDGAACNGLDVQTARRPEPACGFASLSLGSGAATAAEPASAVVVAGGEVTPANGDHRVEAHAIGAVGCREVSSPGMTALGHGANWAGNAAGERTRGQCRDGGAAGPSGRQAALTPVLLLVPLTLGMDKINTVYLLQLQQILSWPQSVGIVGGRPSASLYLCGVQDSSFLYLDPHEAQPAVRWGIGGADTGRTHKNASGSGPAVALPHTSLATYFCDTVRVLPAAALDPSMAIGFLCMGSADLEDLFSRLDGLAKEYSVAPLMTLTSGGALAGAGLEDDFEEPDFGPGQPVGKQQQLDEWELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPG4</accession>
    <name>A0ABQ5RPG4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050508">
        <property type="entry name" value="Methyltransf_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912:SF68">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="179"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="354" mass="38821" checksum="D66548A21966B691" modified="2025-10-08" version="1">MQVAVKPFQQARTVACKRRTRIVCQAQGTVSPVRQFNPLLMGAVEALFKFPPFFSAAAKNARNIIVKRAEALGLDWDGAMRDMKAQDWNARMKAVINPSVTYPAYYTQPFHAYSQGNLCLEAALEVTLAAKSVHAMVMDPAGKQMDPEGDAKLRASYSARIHECLADLGVDPTSIRDMVDLGCATGLSSLELLRAFPGASVRGLDLSPYFLAVGRYEQEQRKAANGGAPESLAFVHAAAESTCLPSGSQDLVSMCLVCHELPQAATRDIMREAFRLLRPGGVMAIMEMNPQSAIFQRIFGNPFAYTAFKSTEPWLQEYLALDMPGTLREVGFGQVRTRESTPRHKTVVALKQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RPX7</accession>
    <name>A0ABQ5RPX7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_000979</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000003">
        <property type="protein sequence ID" value="GLI59151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF35">
        <property type="entry name" value="LD22662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="246"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="442"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="175"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="530"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI59151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="561" mass="60054" checksum="67A52375F101DA7B" modified="2025-10-08" version="1" fragment="single">MSYFVPTRFVWRFGGRQVHLCGSFTRWVETVPMAPVDGTPGVFAVVVHLPPGYHQYKFIVDGKWRHDETAPFMPDPLGNVNNWLFVRRIEPATASIATPVAFVDPQHLAAQQAAALQAAQAAVAVAAQQQLQQQQQQQQQQQQQLQQHSVLGGAATVVSQQQQQQSSSVAVPALLPPSSVGLGHHTGTLSAGQQTSPLQGVPHQGDVDMGGTEAVVPPIVIHNPKEPEYTRKKILDFLHSHTAYELIPESGKVVILDLDLPVRQAFHALHEQGTASAPLWDSVERCIPGVISASDFISILRRLRHSVSSGANPMSEVEMDAHTIRGLREEAAAEGREPKRLVYVLPDEDLAKVVARLAANKCSMAPVLSGDPGGPEPPHVLHLATLSGVLACLMRHFRASLASLPLLSQPLGSLPLGTWSPDAAVAHADFLPDSKERRQWRKLQPLHTVTVSTSLTSALALLLEAGVSALPVVDEKRCLVDVYARSQITDLCKGGAYNRLQWEDVTVGQGLSLALNSPVAWTQGQVGVAGPASSNTTSVSGGTGAQHTVPTPGMGGIAAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQB7</accession>
    <name>A0ABQ5RQB7_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40430">
        <property type="entry name" value="T. BRUCEI SPP.-SPECIFIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40430:SF1">
        <property type="entry name" value="T. BRUCEI SPP.-SPECIFIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="417" mass="46573" checksum="BB2A1CC7A3B84D71" modified="2025-10-08" version="1">MLEQSRSQQSGLAPTDTGEDTVLVAEVSTSGRGDSNAAGPDPDPDDPLAALALAASEDLIAQLPRYATPNISSTLPVYSNNEQEFILRTFAGHNYDMLRHLPTSVREQQVNASRTARQEACRAFLMPGLLGSQLRLKSNPVNQQGLFTAFEYIPSRYSLAAELASKERVANEATRLTIGRGKEFIPVVAPNLQKQHEIGPKVGYVYLAGPEEDQETMRQANRTYHEGAFIQQPFIPPGTQKVAAEVPTRLDAWAMMKNLKRVLEADWEGAYISIFENEHDCWVVCFQEETVDGLDGLAAYMNVFIRTNRVATEYRLTKVVEFWGSSPGDGCVYYVMRPPWVAHDKVDTFFKLHPEERNYQTSFSVVEMEEERKARKKAVDAGQDPLDVDQRFKQMSVKAQHAAPAPDLPPARPARAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQK9</accession>
    <name>A0ABQ5RQK9_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_001815</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021881">
        <property type="entry name" value="NACK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF23">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11995">
        <property type="entry name" value="DUF3490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="455"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="619"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="730"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1156"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1203"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="190"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="839"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="469"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="644"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="790"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1203"/>
            <end position="1223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI59829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1300" mass="138812" checksum="1E74B44A69C8ACE4" modified="2025-10-08" version="1">MLKAVESRRQVRETKMNQKSSRSHLVVRLYVESRPAPISGEDDDDNLISDDSSACGERSSLRGCDSLKAQAPVLSTINFVDLAGSERLSQASMADDMDREKLRQKEASNINVSLLTLGKVIRALGKRGEHVPYRESNLTRILQPSLSGNSRMAIVCTISPASGSVDNSRAALHFANHAKAVTMRPVMNEVRSEQALIHKMEAEIMELRRKLNQSGNKQLLAEKDAQIKAKEDQMRQALQEHQALKRRLAQMEKFIIRGGTTSPAPAVRRSFDDLESLTTSLAAGGIGQGIFGRGLGGLAAAGGGAGVRASWTPTNPSPLEGRDQPSTGKTGKGLGRSCVNPNRLLLDYVIPPNVREAVNRLRTDHAVFRMAASPANGKLGSAPDPASGFNLRTEIGKLQVPEGQKAQEALSALQAEVRYLSQQPEAAAAFAAAAGATPDTADRLLGQLTQMLKTMEQRQQDAHSQTPGSKATNAAAGPSASAAAGERGGSSGGGNASLAASPQPPELLSKQQQVNPEADASAIAYLKSEVARLERLKEINLQTNLAIESLLRKVEVLEQAGLSGSAQLDDMQSMPSVVERLHNALQEADALEEGEELLDEMAAARDDLGGITAARVNSTCTPAQRQSAPADDGEVTSAPRYDSPNATAQATTATTAAPNPTPSRLTGSKLPREQLDVVDDDEDSCAVLIKSPRRTLAASPHSVATASAAACAGPSNLTPNSRMPPAGCTPAGVEGIQLHGPSGSRPVDAAQHRGADSPRQQGPEGAEDLGLRRMRTPSVSVHASDMEPVSGGRRTASASSRGGLLRWDTERAKRIISREKDAIHAWYAAELSRLKAAFREEAQADVAAMQEAVTKYRAMFEETQERVERLNVQKQLLMKQVLHLELELVEAEQQEVQGRSEVAALKEELQRANESAKAAHQLARQAQAMALQHAKAAADAQLPSLPAEDPDDLEDWVQAPTCSTLLVCICDLWHKLHVPLVYRSRFFLHFRSKELIYLQMEQCRLKHRLEQMEKETDYLGRSKPLEKAKRALELERKVLAQGLRHSCTDTEREALYKAWEVPPEKKERKMRLVRKLWDPEHTKDEASMEMCARVVCTLAGPDATEQFMQLVFGNTAEACARPTQVGALVSTLVRRVTTPRPRKSLSVSGAVPTLTATSSGFYNNPGNAGQSNGSVTPRRQSTIMASIAGRMGSIMGQSGKPLGAAAPATGAGMTSAAGTPGSAVSGPVPQLPPNVPIVQAAGPGAGLGPMARGLANSGAFPNAIPPLPPPHPAGMMGSGIQAGPVSTRGRATYISGSSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQQ6</accession>
    <name>A0ABQ5RQQ6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046495930" evidence="1">
        <location>
            <begin position="34"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="323" mass="35846" checksum="F162088AAC3C3ACA" modified="2025-10-08" version="1" fragment="single">MWFSVAVKQRTRLGAVLLVHCCLATAIGRSADADVGLYGPWDPTEDLNEQHKALLRFILSGDTSFWSRPAVAYRIGFGTAPWRCVSNCQTQYYVSAVECAPDCTTQTYCEPGGAALSDSNTTCCSLSLLDQSYSFSHPPNTTTPNWCSTYPPWGPNARPSVCDFNVYAARGTAPPLGANDPFLAVSCKRGADTATFHVTGTQYRNDTAKRMVYDNDITQRNVSRNIVSRISLRHVAAWHHPSININEPPQFSLVDDLVCLPLEEIYFEDVYMRSEHEVLAVLPDRRGPNGTTFDLFDSTTWECDRMDGQEIDMAAFGFPGKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RQT1</accession>
    <name>A0ABQ5RQT1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_001972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000004">
        <property type="protein sequence ID" value="GLI59925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI59925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="615" mass="64292" checksum="68C316C440963A52" modified="2025-10-08" version="1">MAEGLARSSGTSEDILQRVLGQEFLLKCLRKAKFSPDLLQDTADACEINDLNALVLGSGLLSVSEASEKLFLMQDEASTVVAVCRKECLRAGVNFGDGSQLTAEELAENDEEEPAAPAPPPPAVTPTPTPPAPPAPLQSLAPAPARGIQATHIPPPPNHSQQFRPSPSLHEKKPQTLASKAASVPLPPSVTASAVVTLQPFASAPAAASSMLPKPAAPEVSTSKETGTASRHSSSTIPAADLGPRRSMTTTSSVEQHDPNFLIKPPVMGSALGSLPMGSSVLVTPVASRSHIPKPMSVTPATPATGGSTPRERTAAKPVIRASPATLATPPTSSGGASEPGSRPTPSMNGRHTPAASSSAAKPPRPSSAPLSARAASTTVPLGKDRPMSAAVITPVQPTRLPSYAKPTASHKARVTKEDTDVPSTSSPPAVTFASTNKPLRRIQYMGPRPSTSPDEKKEPKKPIFDNIRSNLLRPTVAFLAWSSGKSKFAKDADLRSSQSLQSESIGQPIRGRVPNSAPPKGVIRELSATTASALSPSVTQSNSIIMTTPEPFRLASAERHLKAQEELAKKKEKARLESHIPKFRASPTPNSVERTIQSGKSELKRTTASAQTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RS86</accession>
    <name>A0ABQ5RS86_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002443</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="179" mass="19637" checksum="1BDB7913246C38EC" modified="2025-10-08" version="1">MFSVTWRTHQNQSPKPDCDFFRRSKCEAVQDMTPRWGTFGTWMWGTGKCGKTSEARRSHVHNVDSLVSQLGPHLAPLPADAKACLLCVARRRGVLRNRVLLALADEGWTLMDLAGATALTERALRQALLWSPHLRALDISGLRGTGRFHRRTKGGEAAYRGKVSASIQGAGRPASCQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSF8</accession>
    <name>A0ABQ5RSF8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_002533</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00299">
        <property type="entry name" value="GST_C_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050983">
        <property type="entry name" value="GST_Omega/HSP26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968:SF14">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="133"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="221"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI60527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="531" mass="58636" checksum="473D3EFBF8E4F168" modified="2025-10-08" version="1">MQGLVVSSGSWTSNPILFSRVQPRLQPASILYRGLPAHAPCRIRAARREVKAMSILQDIMRGFSGGSSNSTDDRRPAAVLSFSDNAPTWEQLTDIVRAQHIEFGVDFWADPNEGSTHPLALKRLFGTTEPVRVKLYRDHAAWCPYCHKVWLQLEEKRIPYVIEKINMRCYGDKPPSFMAKVPSGLLPVIELDGRVVTESAVIMALLEDTFPEHKPLMPPAGTPARQRADGLMRLERKLFSDWLNWLCSDWNHDRAKKQFEATMDLVATEMEREGGPYFLGPDISLVDITFCPMLERIAASLSYYKGLYIRSQGRWPAVDRWFAAMESRPTYLGTRSDYYTHAHDLPPQLGGCAMHEEGVSVAAAIDGIDGRAWRLPLAPLSATSLPESYSPGDNPPLDRLEAAAKLVRNHEAVVKFALRGPGQPGPRPVAAPLADPTAIPALGHVAEADAALRHVAHALLVGVEAKQVSPQALQTSTSTGLPGKAVVSAAEYLRDRVGVPRDMRYPAARQLRAHLNWLIDSLRDETGVATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSQ0</accession>
    <name>A0ABQ5RSQ0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome assembly chaperone 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_002767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37227">
        <property type="entry name" value="OS01G0219000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37227:SF2">
        <property type="entry name" value="OS01G0219000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI60616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="30911" checksum="F6D6196114A2C8CF" modified="2025-10-08" version="1">MDIFEQADAFTPNRAVLQEEEEAAASCADSYLRPALCVWNRRLSKQLYPDGMKCELLIISTCPTSCALLRAIGNKQCLGAVLAPGVSLAGNSLASGAGDPLASDKVCPIYTLRQAPPATAAFPGSVLHVDVLLVCCQYEVARERAPVWARSVLRQISWGHAVFLGAMAAEQFRGQGDASQEELIFALRTSASQQRQLRAGARGPVPLLPTGTVVGGLPASLLSYCQVRGASADLLVMVEMVLEGRPALLEALAAALGQLLRSRGLDAVAAAMYNGAVLAAARKGLGTAAACDAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSS6</accession>
    <name>A0ABQ5RSS6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30566:SF5">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30566">
        <property type="entry name" value="YNAI-RELATED MECHANOSENSITIVE ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="114" mass="12330" checksum="3BA3D253FFFAA208" modified="2025-10-08" version="1">MLYRYWCSISHLRLVGVQISPISLASSKTGLLRRFATWVLLAAATVLVVQLLGVDVRSLLMVTGGSSIVAGLASQQLLTNAVSFMQMHPNCYWPRGAVRRAINCREGSLSAVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RSX4</accession>
    <name>A0ABQ5RSX4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein 101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_002271</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG101 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000005">
        <property type="protein sequence ID" value="GLI60201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012445">
        <property type="entry name" value="ATG101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292:SF0">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07855">
        <property type="entry name" value="ATG101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="251" mass="28373" checksum="8C46F5EB129F816C" modified="2025-10-08" version="1">MANCETHNLPVLELEPHQIREALRCVLHTIIFTRALGYVVPKDVDSELFDITYVHCGDPVVEAKVETRITDFCTQVEKKPAELHQLQLSFYETRRKQAWFGTQDERLYWETWVISVLVLQPDVVLQQQQQQQQQQQQQQQQQQQQQQQQGSFGAGTGAAHQQQQSQPHPGTSQVRAARKARLQSALEEQLAMVVRRVNDRRDHIPPVLSASAVTFPFDISFSGTSTRGSLQGGLQAVGKMLMKATPPPVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RT08</accession>
    <name>A0ABQ5RT08_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_002502</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="33"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI60373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="264" mass="30487" checksum="0CBA89A5F46F97A2" modified="2025-10-08" version="1" fragment="single">AFRRMAWSAVDFLVRLVNRALPVALPTSSVRIWWTLWMLTLVLYSAVHVPLQIAFQSWRQSKVVIWLDWMVSISFCLDILVNLRTSIATPQGDIVRTPQIIALHYLRGMFVLDLVSALPLDVIVNISTHGDAHEKLYWVGLLRLPRFVTMLSQLDIVVPVKYFNLVSVTKLLVIMILMTHWAACVWYFLSLEIQGKWPWIFTVDCHCHGDMDKYLYAFYRSFLIMLGDRPPTNNNVERVFTLVLLFLGACFYAIVVSQIDGHCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTE9</accession>
    <name>A0ABQ5RTE9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003091</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052416">
        <property type="entry name" value="GTF3C_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="293"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="400"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="603"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="731"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="460"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="490"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="603"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="744"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="754" mass="75898" checksum="07697DB705076084" modified="2025-10-08" version="1">MSAWVAPDGTWAVRPEDVAVLDTAAQEPYNSLLTSTSKLLKAPGNTYVAPKAPTSSGPWTYGSVDECDKTGVVLQLRFNTSYDIVSVPPDPRTGHGGQGNASSVANDNRNGCVVDAVTFAGGPVWCADFCPPPRGVPLDAFATAPPTDTITTSTAATTASPSAAHTKTFSPVAAAGVAAGVAPPAAAAAAAVTGVGDGSATFGYEPRASNPASLGPAVVEYVAVGAHPPGGSTNLTNVALRGPGAVQLWALPHSEAAPACPKGLPRCAMLLVHDGKVTWDVRWCRSPGCIFQQQQQPPSYKDEDWHPPAGNRATTPSVRPTDDSGGITSGYRVSGGASLNGVDGEQVVAGSAAGSGSGAAVTGGGVRSLPVLGLLAIVIGDGSVVVAAVPEPSALAAVVDSGGKSAADSKRDDAAMDTSVESVGPVDDNEGQEDGDNGGGGSRSGPAPMEGVEGGGGGLATAAAADGTDVAADGAAAGADETAANVSERPPLPGKSPGPPPPLVRLVRPAVLLRREQMGGALASCCDWHMPDPRVGPPQLLVGCWDGSVALWRLPRGGSTGLVERTQLLFHIPADVLPLRRVISNPIWDTDFLLPLGPSTDTHEVHAEERGTIAGREGRPGGRGASTSSSAAAAHQSIAAATAAAAMGCGLGGRIGAPGGPAGHLFCTTGYTGRVKIWDDRDASRPLLDRMVHRSYVHDAAWTRRPAALTLAQDDGGLRVVLLDAAAAMSGGEGSGGVGSLTFRGKRPRRCQNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RTM1</accession>
    <name>A0ABQ5RTM1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_003213</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000009">
        <property type="protein sequence ID" value="GLI60960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46546">
        <property type="entry name" value="SHEWANELLA-LIKE PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46546:SF4">
        <property type="entry name" value="SHEWANELLA-LIKE PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="3">
        <location>
            <begin position="123"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI60960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="653" mass="69651" checksum="D475EFE183F42BB1" modified="2025-10-08" version="1">MDCRRMHQRSLEHGVSCRSNWSTARKCIILVLIFGITVVVFHSGRANIAGRVNLNSSNDIILYGLPLPEFDPGLDLSERFSLIFPSTKLTSDNGDSNIRTNRSSTISAVNAASDGGSTHSRLVVIPDLHGDAPQAFRALRLAGLVSAIAAPAAAEAAAAAAATDPNGGGSPAAATGTANDTQIRESDWRWSGGDVVLVQLGDVVDRGPDSLALLIRLKGLQMQARAAGGDIVMLLGNHELMNIYHDFRYVSHSEAIDLAVSERLKLSPSMAANSTTSATGNGRRMAEDTLNAGGSAVATEQHPEAVKGAYGNFHVVHAVKDFDSHVERELRQETGLEAAAEDVSLAAETHAVRRGNAGDRELLYASTMSITSTDGGHLDLRGRRALLQQQRLSPQQRQRQEHQLFLSGLKIWRDTLAANTPMGSFIRQRPLAAIVNAGGCNILAVHAGAFPWMLRAVEILLQGRRDPGEGAGEGGTGGTGGTGGGVSELSRGDPLILQPEQYIAGWNAAVAQALQNCEGRRCSRHDSISGDGSGTDAGHNRRQEDDNYERFRLALADLLLDGEGAVWSRRYDKGPRQKVCGEVLEMTRRLGVQKLVVGHTVQWGGRVSSRCGGQLVMADVGISRAIAGEMAVLQCVAGKLDVVYGNGRMERIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUB5</accession>
    <name>A0ABQ5RUB5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Galactose oxidase-like Early set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003097</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02851">
        <property type="entry name" value="E_set_GO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.80">
        <property type="entry name" value="Galactose oxidase/kelch, beta-propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037293">
        <property type="entry name" value="Gal_Oxidase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009880">
        <property type="entry name" value="Glyoxal_oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015202">
        <property type="entry name" value="GO-like_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208:SF21">
        <property type="entry name" value="LOW QUALITY PROTEIN: ALDEHYDE OXIDASE GLOX-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208">
        <property type="entry name" value="SECRETED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07250">
        <property type="entry name" value="Glyoxal_oxid_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09118">
        <property type="entry name" value="GO-like_E_set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046378372" description="Galactose oxidase-like Early set domain-containing protein" evidence="2">
        <location>
            <begin position="35"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="Glyoxal oxidase N-terminal" evidence="3">
        <location>
            <begin position="146"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose oxidase-like Early set" evidence="4">
        <location>
            <begin position="521"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI60878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="636" mass="68975" checksum="44E802E86FBC3DF3" modified="2025-10-08" version="1">MPQLAPFCRRRVMGVTSLLLASAVSPLLFLLCLAASGSPDDARASAQIDLHAERTATATVLPLRVGDDGGIAVGGRRLQLSAARTQERVAQQLEPLVAVTYEISSLRPPVISIALTHVPKAGPRSRTYFAYSRARWRMDKQSAGIFDLNKNESNLVITPFDHFCNGPILLADGNPALVGGYDEPANKAQTDGRKWISIYDHNRRSMTSMTSMFYQRWYPTPCLVKDNKVLIVGGTYNIDKGPQIPVAELWDPTKPNAGTEQVPLPPTFKKNAGFNWYPFILMLPRGEILWWGDRGGSITNSNWEEIYIFPDLPNNTFPYRTMYPCTSSIVLSAMKPDGKTGEYNDFSIVIFGGAPDGPKGAPASPVSARLDMFYCGTNICDKGWVIEDMLGQRRVMPYATVLPNGKVLVHAGGQAGIAGWKSGKYNYKGILPAYRDLMYNPYAPLGTRYSLSSTLGIIRMYHSTTCLDLSGKVISAGCETCGMTGADAGDLPANVSRSPDGDLDYRISFAVPTEIGPGVERPVIISAPEVITRGSVFTILYTGTVTGATLAAPCANTHSINMNQKVLFLNLVSVSSGVAQIRAPPLSQPMAAHAGYYQLFLLGTQTATGQTYSEGVWVKLADLSSMSNQEKTGGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUF5</accession>
    <name>A0ABQ5RUF5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_003546</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051070">
        <property type="entry name" value="NF-kappa-B_inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46680">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46680:SF3">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR CACTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="101"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="299"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="413"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI61245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="47594" checksum="056D523EB08E9961" modified="2025-10-08" version="1" fragment="single">MGNILAPSGASLYDAIAEGDADAAVEVLQAYPDLTRKRLGTKKRTLYHFCAASGQVSVLKSLCDHVWRTVPDESGELAGVQGCYTRQHSAIRQYINKCDQKGLTPLSLACKKGHGHVVAFLLTQGADPWVKDRMMGRTALHHAARANHPDCIEAILSSDMVQQTNGSDTSPSKLVDHPTNAGYTPLHYAAAAHSREAAAVLLRHGANPNAKTWEMGFEHLQLDRGSTPMHAAGRYQSLDVAMLLLRHWDESLRQLDVTDPRVVENLPRIKPYQMPGVKLNKTLFRILDPATPLREIREYHSATRGNAPSSANQRNRTGLQRQESELSCSGSGGAEESGPGESYGADNPVPERSSPPASVPRQAISATARPGPSAAFKEASLEPGPTASRDGNGAVNVTAADRSSRGSFADVNSKASPVSTPKASNTPFSGALAPLGSEIPQDHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RUM3</accession>
    <name>A0ABQ5RUM3_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_002953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000008">
        <property type="protein sequence ID" value="GLI60727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007174">
        <property type="entry name" value="Las1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002:SF0">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04031">
        <property type="entry name" value="Las1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="731"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="774"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="627"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="645"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="660"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="785"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="798"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI60727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="849" mass="88501" checksum="2DF16C5B0F195581" modified="2025-10-08" version="1">MSGRQVPWASWGEWQALRADLWSTDLQRQEAALQQVCAWRLRGKLPLGVDVTAHLMDVQRADADWQRSVACAVESSVAVATASSQLSRYTSADAAVAAGELPLRLRYSMPLIRLVNGISDSQQRGKVASSVAVLSDAAGLPLALVDVRHEATHNELPSLPLLRAAASSALSWLQDNYWGGQRRVLGAAGERIGSLLSILANSWQAATVAGFSCGAMAAALTLEEEEEEDVDGEIKGSYSGSEGQRVRRQTMSEIRSLVPTSFTQELLQPILDCPVLVPPEPVPAVPGASQQQLSTQQQREVVDLRAWSSAMKHLLRWYPRLRLLLLKAALQRLVAAAAPAAAAACTRAAAAAAAPLPTNTSETREPSGVDQRARAYGLWVLRLLPGGATPQHTLLASQGGKLGSPARKHGRTSGGHGAHASGAGGGGKFFKKATAAGPTATRPSLPEADDSGDRGDTIYLDGPLTLKQLQQLLEIAKAAADLPPQKSGIPVGCPEGCREGAAAIASAPSTSTAAAAANAASATSGGGGGPVQSLCTLREVITGLQRALKQAGSAAGGGGGGGGGMGKKRQKLSALSKADVASAGEADGVGKSEGAGSGQRHEEGGGAGKGDCTDADGEGNPIRRESQPQQMRQQVPQTGSEHQKPRQQKYHQQKQKRHPGLQQQPGLPRPSQRWQRCTEWSKCAIGCLPCPLNPNGRLPPLVLPPAPAASNSRVPLKPEDIVAFNSARAADPGGFDGTTSDQRAATGAATGDRCDVRAVNPTLCDNVDIEYYEEPEETDSLQGLQERDADRDGVSDRDDMDDSDGGVDPEAVDEEGAPVPVFSSLFGALLRQQQGASGNSGAAAVGFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RV12</accession>
    <name>A0ABQ5RV12_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA topoisomerase VI subunit B transducer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015320">
        <property type="entry name" value="TopoVI_B_transducer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444:SF1">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09239">
        <property type="entry name" value="Topo-VIb_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA topoisomerase VI subunit B transducer" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="112" mass="12169" checksum="40657B1178F0DFC5" modified="2025-10-08" version="1" fragment="single">KELAPDMIATHQGDVRVFEGHAFIVEAAVSVGGRDIKPGINVHRFANRIPLLFEGGSDVITKTALKRINWASYKINQATDKVGVFVSIVSTKIPFKGAGKEYIGDDVEEMVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVC9</accession>
    <name>A0ABQ5RVC9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="111" mass="11724" checksum="DC4EFAFDA0E1A9C5" modified="2025-10-08" version="1">MIPQDTRQVISGYNMVVFTGGLTSNDTIQVSLCRFDGSDDSQCPPPSPPPAPPPPLKPTPPPPSPQPPPRSPQPPPPSPQPLKPRPPSPKPVPAPKPPAPKPPAPRPKITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVG1</accession>
    <name>A0ABQ5RVG1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="160" mass="17460" checksum="090E8B4C151783B4" modified="2025-10-08" version="1" fragment="single">YEPRRSWKGHNVFNLSWNIIYSASSVGCMMLSTRQLSVTAYRDCCRSTPVPRRKNIIPTIRRSIYHILFEVMTSSGGEDHVDPESIPRMKFGSGDPHTQTSEDFQTTVAGCDELMAGEAPPNAMAELVNNVMSHKLNSHEARGTGGGGSGGSAGRTPDTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RVQ5</accession>
    <name>A0ABQ5RVQ5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">VaNZ11_003776</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF435">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN EAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="404"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="536"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="98"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="673"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="915"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1041"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1133"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1371"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1490"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1564"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1810"/>
            <end position="1846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1924"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2003"/>
            <end position="2037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="673"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="695"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="939"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="986"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1072"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1086"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1155"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1201"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1371"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1490"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1573"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1826"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="2021"/>
            <end position="2031"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="GLI61419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2037" mass="212108" checksum="27A687C60138E7B3" modified="2025-10-08" version="1">MAASVAFLHQVEYEAALNEFEEEAKRQFELHQDEERAEGDETAKGEISRAQSPAVQRQKMQLRRRLLVRPGDAEKYDARKSTSVASWLSVQESAKNVSASAQDRWQSRGAGRSFGITRPSQWQDKQSRQRPQQNPNHCRRRQSILLITSAAMVADVHPRHSSSITSRTGIWHRILHLHAVIDGILPIFSPESFLRRVWDLVIMTLVIWTAVTVPLSVSFGLPDTPELQVAGYAVTAMYALDLFANFRTAYYNHQAELVRDSAAITTHYMKTWFWVDLFGTIPFDSIVLWTGALKDVHKGTSNAELAALGFLKAPRLLRLGRLLRFLDRFKNAKIFRIVQLFMAMILFSHWLACTWYMMYRFGGKDAVDDWAFEVAVFETPNASVHATSSYHANSQTADVSGTTLSMYVVTYYYSFLLLVGDNVTAYNNFERTFCVLVLIAGTFFYSAVVGQMATLVATMNVAVNRHGQKLLMVQDALRYAGVPDGHSEKVQKYYEYLQQRSHPGAEGMQFLQELPSSLHVSLCQFLHLRSLHKVPLFKDCEEGFMSALSLRIRMISLSPNEVIFRVGDVGKEMYVIKKGCVAVTSPTQTMWGLLQPGEIFGEVALLSTGKRTANCTALGFVDLAVLTGPDLKAVMRDYPVSASIIYERVAARAAALQSKSKMWLDIPADEEFYDCEEPNDDADGGGNRGDNAGNDDGGGDGGGGDRSGAAIHNPSGRIIASPEDGDAGSVVTRSSITSGSLTSSSIAENLIRGAARGIDSGSNIGGGDGGDDVDVDPMSVKNICIAAFGGCPEGDSATTKTVACTSGAACDDSGCSVGTNAAMSCSETLQAASTMPRTPSLTVAARPGGGVPRGMLAPFNGRVLCMAWCDIVNQDADAGAAPEPAAATAAVHYPLASHDNSTTILPAKIASLAESETRRQPLSEIGPPEKSTPPPSPPSDCCTSSPRPARFLTREMTPLPITSEPPASSPAGPQLKPAWGYPAPSPQAAGPAAAPATEGNASGGCSSPRVHTSLRPAQPLAVQTRIARSSPVNFATAAVTSVARGGPTSRSGSPFDVAHLHPDRAPGGGAAGLASDSAVAAPTPGTYRTANSRRGSSPSDDFMPPIDAAVSNEVALNENGGDRAAKLIAAKDADEGARQVPHQTHRMRQGKSKISVSGLAASGSGGAAAARATDITRSRSPGRSPQHNHQLAAQHLQRHHITADDGSGKDDEATPATATTPLEPFGLPLQTSNGLIDLVEGTSGSDANIAWRHPTSHSPLHREGSVCGSGGSIRVSDGASMCSDRQPPAVMTAACVDDCIRNSAEAAPVTTNGEATSHRSRAGPVAEAVDLSTPFTLSSAASASPSSQQAPNRSRNLSMLSGAANTATGGSADACASAAAPSATSPPNGSTRDAVRRRSSFLRWLQAGPAPNTVPVAAASGSAIACETAASDSASPATSVPNPVATLLHTNGAAILRLSRRAIGLLPHVGGPSGNHSSGGAVASAVGGMSLSQPPSASSRRTSCQSTGTGTIAPAPAEPVQVAEEELQWISTRPSYGTLCRGVKTRRSSFCSILEEELPALAQGPFGPRHHVPSSGPMSSSNRSNITDCRPEEDSTPKVCCSSDTNTGMSVGTSTGMDMGTSSGAGLASAAAAVANTGNRRGGPQLPHNTMSHPHCPESSTATGSAGNAIGMRGILPTCSGHVSRTPLPPAPPPDGVFVPRDSWEKLVQALTWFESSLQQLTDLATAQDAALSRLEQRAERAAALGGAGHGAVDDGILSGIIPASIFADAATSAAAAAGSGNGAVAAGVGGSTQTAAAMLSHSLSSTSYGRSIPASRAATPGGGASSVNSLSRSDSQDANTQRVAASRIRSAVRALTVSNRMRNSYGVGVARVSVTSTPAPQAPGIPDAATSSPTLSPSPLMAPLRSARASDTPDGFAGLMSFRGSGGSGDHQAATSDLSGGDNWAGAGVSTWDLDVLMGHGRRRRAGSASGLPIGTQIRQGYNTSLAAHGSGLRAALKGVASMPSPLQESPSLLKPPSGRLSSHQSSWTGNRRGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW53</accession>
    <name>A0ABQ5RW53_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pherophorin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="35093" checksum="3B83628C192979DB" modified="2025-10-08" version="1">MVKCRRKTQSKSALKMIGTFPKTRGLAWTAGLALLVALALEGHLASSVSTPGPKAMSPRPPSPRPPFIKSPAPPPPRPPYIRSPGPPRPAPPRPPYENNLSALSPHLSPPRLPQYYIHFGRCVQAFQRQEYGNWIMIRSASVQTSSDSIVLRLDTRNMRPVASAFGTVKPNYFPISHLYIAYFDIRGVASWTPTLDRTPYKETPSYYSIKVNGSPLGTVRDMKGEGDDGPRAVSYSLKGFLNGIIVPSTLPYVIEIVKVCTLYGISCEGVPDPRDVFVSLRGFTAYSLWTVNQPLRVNGTYFRDHVWCPVQTVIDVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW70</accession>
    <name>A0ABQ5RW70_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein cereblon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_004389</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRBN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15777">
        <property type="entry name" value="CRBN_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034750">
        <property type="entry name" value="CULT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51788">
        <property type="entry name" value="CULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="10">
        <location>
            <begin position="123"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="CULT" evidence="11">
        <location>
            <begin position="350"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="496"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="502"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI61878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="546" mass="59740" checksum="84D1BE482BE83F28" modified="2025-10-08" version="1">MLKWTLEDVISGINKLGQRLKTWLIQASRAFYRCETRRMAEQSGSQSPLAAAGAESSSEDEDEAARGGMERVSSFDLDAADGGTEAAPRHFDPNTVAQHRYLGDVDELAGSSQLLEEGATYVLPLFPLSGVVLLPGEVLPLFLHSASDILKLERALRLPAGEPTARLIAVTHESWHSILSTVGCTAEVRRMRRNFELRDGGQAAAAAADGGPWRVSVVAVVARGRQRLLLDRAALNDTFRVRARVLPEGLAEAPPRQMVSGAAFWDSWATRPFDVPSLAVRVQQLCSGVLPQIARKFGSSSRLHGPLSLQRYTYWLASNLPLGAERRQQLLECLGTAERLRLILGWLTRLGVLACKYCGATVAHCSSALLMSSEGAGGAFVNAHGFVHDIATFRSVQGLSYQGQPETAHSWFPGYAWTIANCARCRDHLGWRFTACSEGLRPSVFWGLRRSAILCPELQRPRTGGGLLRGPEELLLGEDQRPVLMGMVEAIGLAFPMGDLRTEDEEEGQEGEDEDNDEEEDDEEEEEDDDEDDDEDIEASGGETEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RW78</accession>
    <name>A0ABQ5RW78_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">starch synthase</fullName>
            <ecNumber evidence="4">2.4.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_004385</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18189"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.21" evidence="4"/>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03791">
        <property type="entry name" value="GT5_Glycogen_synthase_DULL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005085">
        <property type="entry name" value="CBM25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46083">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46083:SF5">
        <property type="entry name" value="STARCH SYNTHASE 3, CHLOROPLASTIC_AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16760">
        <property type="entry name" value="CBM53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01066">
        <property type="entry name" value="CBM_25"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate binding module family 25" evidence="10">
        <location>
            <begin position="518"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding module family 25" evidence="10">
        <location>
            <begin position="720"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate binding module family 25" evidence="10">
        <location>
            <begin position="878"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="241"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="95"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI61875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1428" mass="152798" checksum="3E4A9ADFAD3C130D" modified="2025-10-08" version="1">MQQPRPPSATRSGMRSLGSTSLPMSIPGQPGSVTAQRTLASKQCPFGVSSGIRRGAGWRPHGPPNSGGRTATSGRIGVQAPAGTSSASGAKGVTAGPAAKTSVASAAPPAVAPPAGATTTTTTTTSSGGGGGGAATATAAKPVSVRDASGGIIPPASPADVAVPGTAGNGQVPGVTVRGPSSPVSFDKAMAAKINAARELARRLAEEKQAAKAALEKGPDAESKRAAELASAEAAAQAAKADALVRALRRAESNRDTLQRLRVENEALRELLLEVAVDKEAARARMEQVISGLNDQGLVERAKAAVSAGPVPPPMSAAELLSRPGPLPQHRPVDSVTAKPPDTAAAASVEAQLVAASAQVAETISPSKAPAAEPTATVTEDVELVAEMRSVLQLKAPLETSEDTIKQVVKKAEQSGAAAAPEASAAATAVVEELHLVREGPQAVPAAAPAPPVAAGAPPAAAVAPPAVEVTPPPAPAVPEGPPPVPEPTSAQLQAMAYRAISSNQLIFTWPEEGGLVGRPVRIYYNRTRGPLPPSNRLQVKAGFNKWEEIVEVNMELARELPPGGGNEWWLASLELPSDLFRLDYVIIDGVSGSVDNNGYRDYRMKLVDAPTEDEVMAARAEAYRRFDEDRRKLIEEEEKRLWRLVESKALDAAAAARVEFRRRRDSELLEAARQVVADRRKADITAGPVANKVEGVYVWCGGPPRPGQRSFLAYNKVHSNGAGGLMHASGVRVHLGYDGWWNKITQTTDLAPLTQEQLSAHTLSSSGGVQWYGAWIDVPYSAAVLNFVFSDREQRNWDNNGMRDFHTLVSGAHSGDELVEMLHAAMKRDSADSDKEVEDRAALRAVRKVEAKGLTMRKRREVVHEFLYTVPLLPRAGQAVEVYYRPDATMLRGRPEIWLRATWNRMGAAACNAAGGALRPVMARLEPCLPGGLGFFRARVQVPAEAGGMDLSFSDADSTSGGFVDDNGGLDYHIPVEGSATPRRSLNIVHVAVEMAPIAKVGGMGDVVTALGRAVQEQGHDLSVIVPKYDCINYKLVDSLVQENGFSWGGTFVRVWRGFVEGLPTVFLEPENGMFWVGCIYGRNNDAQRFGFFCGAALQYLKLTGRQYDILHCHDWQSAPIAWGDLGAAKTVFTIHNLSYGADLIGRAMQSCNVATTVSPTYAREIAGHPSVASHLNKLYGVLNGIDQDIWDPSEDPCLPMHYSGENVAAGKEAARRLLRQRLNLAQSDVPVVGCVTRLVAQKGIHLIKHAAWRTLERGGQFVLLGSAPDGRVQGEFNALRDQLARAYPDRAALVFTYDEPLSHLIYAGSDMFLVPSMFEPCGLTQMIAMRYGTIPVVRKTGGLVDTVFDVDHDEDRARVQGMETNGFSFEGTDFAGMDYALNRALSCWYSDKPLWHSLRRRAMMQDWSWNSPALDYLELYYKALKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWL9</accession>
    <name>A0ABQ5RWL9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_003792</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000010">
        <property type="protein sequence ID" value="GLI61432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI61432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="142" mass="14972" checksum="7A14F765EF24A931" modified="2025-10-08" version="1" fragment="single">LQVIVILGLGKSFFPCLGSPAKMYSGTVQSGLVRDQEYAPLLFSVQPLDQATPRAATPLRADSSLSTQRRSITQTPGSMATDATPPPPPPIMRLTEDVVMDEPGSMGRGITPPRRQTPQQQQPVSMPAPATAATTTATGPAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RWX5</accession>
    <name>A0ABQ5RWX5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_004395</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000011">
        <property type="protein sequence ID" value="GLI61884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF117">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="2">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="AAA ATPase AAA+ lid" evidence="3">
        <location>
            <begin position="129"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI61884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="21243" checksum="72091AE1A1EE41FC" modified="2025-10-08" version="1" fragment="single">LSCAQLFSMYVGEGEAALRDAFKRARMAAPAILFLDEIDAMAGRREEGGGGGGPDAGVRLLTTLLTEMDGIELASGVLVLGATNRPGAVDPALLRPGRFSTILLVPPPDTAGRLAALRVHCRRMPLAEDVDLEAVAEETDMYTGAELAAVCREAALAALREDLQGASQVAARHFTAALRTVMPALTAEQLKGYVEWGRGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2025-10-08" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYF8</accession>
    <name>A0ABQ5RYF8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">VaNZ11_005333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="GLI62658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="193" mass="20685" checksum="0632A757C45B0BEA" modified="2025-10-08" version="1">MRSSACSTTSSTTSLTLRRRTATGCCCRKARKLPPFWSPFHCRFRSCWRRDSKTTSWAPLHLPSRLRSCSLLKEAPREQPATNGLSDMFVAFDGSKHEQALQPALTAAEKGWLLRTNEAKLSSAAATALASNAGWAKAVGKALSSANVGCVGLELFLPADEAQTLKNQVSQLGGHASDSELAGKAFRTTGLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYK8</accession>
    <name>A0ABQ5RYK8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_004896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="914"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1040"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1134"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1172"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1211"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1443"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1683"/>
            <end position="1706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1789"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1843"/>
            <end position="2010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2336"/>
            <end position="2383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="546"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="696"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="928"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1059"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1134"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1846"/>
            <end position="1859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1867"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1914"/>
            <end position="1930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1992"/>
            <end position="2002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2336"/>
            <end position="2362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2371"/>
            <end position="2383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="2383" mass="242278" checksum="AACA8A65D9E24769" modified="2025-10-08" version="1">MEYKRFLDGIAALEGIILPATPPPSGKEGLQSGSAEPSDSSNHSSDQKPSSSVNYSSACLHNEGGTITMLNQLAVGASHLSGVPQGSGPALAFFLQLPTSCHQTLNATVIPQQPHKRHTSQVRPQLPSSAATELTGAPAWPGDRHVLKLSTETSPNLPNTLVTGHSNAYWDPNGLRKAVLLLTFNTPAATHAALARLHAVALASQGRAALVALHPVPLVAGVLANAVTTGDHVGSEQAAAVLLALLANAQLPLPEDVAGALVIPAAIQLLGGRAAADVAAAAAVSAAAMAADAGGTLSSTALHVRLQTCTTAAAAPSLPPKMPYSVSATIAPAAPISGRLAAAQLLLHLATSPPSLAADSASTTVVKAHSSGQPRPAPISNQSHGPGAGAVCDQLVAAGVLEACCEALRASVGLIDAAGATELQCCTLQLMAALTEDATGWPWQWPVVRAATVDGCAQHAVALMQLLDGISSSGGGSGDDRNGGGIFDGGAKVEAQAHAAARSVVVHLSRGPLLVREALVRAGALLPVVEAVMMWPPPLPPPPPQHQRAAPRAGPAAGQGQRRHVSICLGILLNLSSIPRVRVALASEALGPFLSHLLGLLEQQPWEAGSGVGAGQGEMASTASHGSVDVRNVLLEMLLNLALDPQCHEGLAAQKVEAILVRCISASQQLRLDWPGPNSMLLGTTAEDQPSRHLLPPSSSPSQQQQQSYQQSWRAAAALRLMAALAADPRFSVPRPTMQVGLASVGALEVVAHEVRFAVAALPDPGAATAYDLAVTALCALMTGNGELQRRVAGMPELLMALSAALPHPVVARSEALLQITLAAWRLITSPLAAPGADPATARSLLSGLGLDPDALMRPPGALGGSGNAVPPAAAPPLLLHSRSRAMYELTMRPSTRLHRRSILLTAAEMRNRLSSSNGESSHGGPRASRDQRRTGEHERVGELAEVSEWHRGAAQGIIADHRLAAGAPRSHTISSRKGGMEGTYGVGAGEGAPGAANAPVPLWTGPLLPGTPLPPPGMPPPDVEPIRLPSCVLTVLESRPSSATGKSGVGAVAGGPTKSMSQTRSCRTPSRTSAEPSQVVKPAEPPHRAVFVSAESDEDDGGSSASPPTAIEVLGAGVAKGSGSHRLAATPPVAAAVQTTSPPSADLTQLPPLPGALLSVAPEEMEADINGRREGVGSSSSSSSRQRRSSEGSGSGAGIFARAALEADQAVRTSDRVNGDTEEVPPRPPKSSVACPWSASSGRGRESRSAAADSAAAVSFGSLAAAGQVPKALVVNPALEMSPPLLCLITNLQALLARPACRAVAALVLVQLARLEVVRSSAVLAAGWGALMEAVTSSLTGMTERSALEPDDQLDTFAGHSTAVAASSSSSVSVPTDPGDQLALLEVMDVAAGSPGEGRAALTSRSVLCVLCDLACGQGNLAASLVLRGDLVPRSGLVFPPQSQVRPFRHHPNHPNHPNHPNHHHQCVVSSKSSRHQLGGDSDSLRDLQLQQPYLTRGRTLAMEILGELIALPPVAAHLQKGRTGAMLAEQQVSALAAAPEAAMSSHARALSVPPPLVAEEQQRLRNAAARLLLRLAENVSCAREFLSRPGVVSELLDHLSELWDTYGPALVPAAASARLPRTGLSSGFTLSSRGPITAAAAAAAAIEANGGISDSTSDNNLNISLALSNRRNSALHTGSRMHLLSSSGGGGGASRTPPSASNLALGSGTSGIGVGGIITASPSGRFAALLPSTSPAAPDGSGGGSGGGRCCSATGLPGTASELGAQSSGLAPSCHQLQLLQQLPRPQSPALGATRGGGGTSSLTGLMPRGVTTRMSLHAAVCTEAAQWTSPAQHAMKALELSAERQQPPATASSGQYFRTAPAHTGSTVRPSPLSVGSQPCRTESGRGRGGYEGSDSGMSDEDPFTVLRDVAPPPLPTEPPPLPPPPLGHDQQQRQPQQLLPPPPLLRSGTPSRHRSGRMAEVAFVPDGDGSGAVCPGAERDREPDDGDGGGGGGGGGGMDDPEGRGLMQNTVLRRGTRALAPPVAPGAGLPPGVAAAPCTAAAESAARCAAELLRLLRLLAEGDVDAARAMATLPAVELFVDLLAASNGPLVLAAMQLMEQLAGRQDAHETFWDCDAVAQLVAHLQLRSAAAVEAAAEAATATIAVADHALRYGNGEAVPSPGFGVTSAAESSAAAAAAAMASDLQLGAARVLERLCGDPDLHGRLAATELPAALVSVVKRSADQRTTSQSQVMGVFTATSTPYFASTAQGPAASRPADSRSGALLACVLSALSSLLECADVRRQARERRLPLHLGILAGSGGEGSDPRVPRLAAKCGERLREEPSRLRTFWSASGAPGPSSSTTAVAAVPNSPSASGICGSGGGGRLPLVSLSGRPHRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RYS1</accession>
    <name>A0ABQ5RYS1_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="149" mass="15454" checksum="751DE66B39D207C3" modified="2025-10-08" version="1">MEPPEGGGVAAIRAARAEALQAALAQMWQPVRCRHWPLTAVVTIPPIPQQRRGAAGRQLTLAACGDAAAAQRTTAGVDRPTACTITPHGAASAQGENSSARDSKQKLRYRSSAEEESRSGSAAAARQQRDGLQALSPYGGRGGCSVQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZB4</accession>
    <name>A0ABQ5RZB4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="552"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1040" mass="107741" checksum="F30464466C8FD7EF" modified="2025-10-08" version="1">MHTAYWLKQFFSCFGTPPEGAPNYRAIPGPEKEKFVQSPTNHFEAPYTRVFEPFPILWAYLSRSERLAFRACCRDSRRIHDSLVTDLYVCDDSAVLSEVPAGRLPRSPLPVCHATAHGITTALRGLAERGCKPHLLLLCLAEGHERRRLEEGIAALSALANAASKLSVVHLCGVPLTPQTARAMKNLLASSELSIQMDMPVQRRLGTASLPVVQGPSAAPSLTSICSMPTHPTLTVAAPRPPPPALPLHLNLYGYDLVRQPAAVAVEMLFLAVAPRLTHLELWGCVGWPSRLSTLLHTCLHMQHFSVSFHQLMPPDSAAATAATSKGLVQRRGPATPVASPLARTSAALATGAIVAFPPIAAASTAAGAVKAIGGMHHLRSLELRGFIVNMTRRTSCDNVGRCTSSRPSATLKAAAERAAIASAAPSVQQLAAALYQLTNLTRLSIDGLNSASPLLTAVAAMRGLRQLELPDAAVPTATDLAAITRAAPRLVALTLGSLGPGTVIDAGASLCTAAATAALGELPLPPLLVTLRVARCRPTLRTMRSLVRTVEGRAPPPAALTETDPSGGAGHSSPTRLALVVPGIDIDMLDVEFDALQGFSRGSDGIMRSTLLPESAEALAAAIRVLAPPSSQMLGKGLATNSLSGFGAMASPSVIEPGGAGAGAGRISSSSYGLGASSSFGGGFSCSLATNGGDRDDGIGTMDLCNGRDGDEAEWASAFGYTITRELTIHNSQGSLCPPSTAQLAPAGPLVPLPVASGLEATPPVAFELDAAAGAGAASGQADGADGEATIDSIQIIATPPLVGHAVWISEMQPLGLTSLELYGIGVLAGDLEAVAELMTLQRLTLASGELEVDALPCLAALPLLEYLELARCALCRHRRADGGGFARPTTSGFIRGSGGDVMARSSFATRQSCASVYDHDGDGSEVRPSGKRALLELCVTAPKLRKVFIVRRMGIVCGTEGTQGPMGAEWVRQRLLAAGPRVRAGRPPPALVAAAAGTAGDVLSIKRASLGRASASSAVPAAAPGAEVELGWPEIKWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZD4</accession>
    <name>A0ABQ5RZD4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichol kinase</fullName>
            <ecNumber evidence="3">2.7.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_005804</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyprenol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.108" evidence="3"/>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032974">
        <property type="entry name" value="Polypren_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205:SF15">
        <property type="entry name" value="DOLICHOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="495"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="725"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="749"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="792"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="867"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="902"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="929"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="534"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI62950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="975" mass="103088" checksum="73ED63CD163453A1" modified="2025-10-08" version="1">MWSPQNVEAGLQAASIFRLGFSLLGPALHGQRGPGAALHALCMTILAALALFLDRRGPSWFQDHPLYMFRACGQAGLAQGLLALPSLATAIFVATRSWWWQQAARLFLASSFQVLFWFAWTTSLFSSDGRSNNISSSNPDGKATAKAAAAKAVSANATAAAPCPAFGGRQGRISPTAAEDSDSNCTRIPSKVRQPESQLPLGINCNASRLLFLTASAVGCPLSAANALLHAVKPPEPSSPQLLLPGSVFELPRDITTSAWVLQGALLLATVPSLDHMASVLPRSFTPGEMSILIQVLLALAAGSCTYLMGASRQGLLHVLWSPHVVQLVGPDPGMHVPAFCMLVLFWVLVLAGCSAMSLQGLGPGGSQASQGEGAKGKESMETLVAESPLQATASASGTPVMATALTRRRRRQLAGASSSRAKRPVALANVTPTPASIELQHPGSDIRNDATDWLITSGIRAAAAFGAVLSFAMLIDLALWVLGRFVAASSQPRLATLAYWFGCLAVTVPFMYVVSRASATRNQQRHQMARLERLEKQQQPWEEEDGGGNRGKEEEAGSGRAPMVTPMPDGAAARHLTSSTSAEPREDLEPHVSATSGYYMQRRNGHVGEEASAVARSSGVRNPCLGDMGDGSRNGGRCGVAPVTSFPPAADECPGNGPGPVESLAAYSARSISSYCMDRGTSKNEVKNSRTSPDSLAVAQRQLPQLPRPVLLDRLFRALAVPHIVMRKGYHLLAIVLFLPAFGWDVRMLQASLAVAGAVLVFVELLRVLGPRQLRHAIGGFMKDFADARDSGPVYVTHFTLLLGIAVPVWLGDSVCGAAAGAMMGGLYGSKAAEPMSSPSAPDDDGPLAPRLSVEAARLLPSCRTVLGLSGLVSLGSGDTAAACVGFLLGQWRLFAHGKKTWEGTLSGAAAMLGSWAVVVWWMDVRWAMSWDMWVALAAVTGCVAMLEAVTQQLDNVVVPLYYFTHLVLTMTGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZP0</accession>
    <name>A0ABQ5RZP0_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">VaNZ11_005832</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI62975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43574">
        <property type="entry name" value="EPIMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01713">
        <property type="entry name" value="NUCEPIMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="4">
        <location>
            <begin position="84"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="GLI62975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="425" mass="46686" checksum="0D99E0524784794D" modified="2025-10-08" version="1">MNNRSSTSLSFGRRLLSVPVAYWHAAAIFLAVSMFATAFLVIAWLRSTQSTAVVLLVHPLSAKSAASVYKTCASSGSGLGKRYLVTGAAGFIGFHAAEQLRARGDVVVGVDNFNDYYPVSLKRARAQVLADKGVPVVELDLNDQEALQELFRLCSFTHVLHLAAQAGVRYAARNPFAYVQSNIAASVSLMEAMRLQKPMPALVYASSSSVYGLSKRFPFTEDDPVTQPASLYAATKRSLELLAHTYFNIYKMSVTGLRFFTVYGPWGRPDMSVMAFARNIVDGKPIRVFRGPNNTELARDFTYVGDIVSGVMAALDSAPLSADPKFPPTYRLYNLGNTKVHTVSEMVQTLQELMAMKAMVRYQPLGATGDVLRTNANITAAHAVLGYTPKTDLREGLREFVKWYFDYYGSDGKKRPADEVGYVPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZU6</accession>
    <name>A0ABQ5RZU6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_005530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
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            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="730"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="815"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="926"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="434"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
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        <location>
            <begin position="506"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
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        <location>
            <begin position="581"/>
            <end position="597"/>
        </location>
    </feature>
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        <location>
            <begin position="686"/>
            <end position="704"/>
        </location>
    </feature>
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        <location>
            <begin position="730"/>
            <end position="750"/>
        </location>
    </feature>
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        <location>
            <begin position="761"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="837"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="898"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="926"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="963"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="979"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1030"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1109"/>
            <end position="1126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI62783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1265" mass="126979" checksum="859A8DF3D12BDED2" modified="2025-10-08" version="1">MGCLCFGSGKQDLSELSAPLDPAVPKPDPDYASNRGQGGGRNGRQAQTSTIGRAAGSRGATFNGPPAGASHPPAELEGATQQRDPAAARPFQRSSPSGAAASAPEGLAVAGGSMPRANTGNRPQPLSIPDSPPGPPAPPKAARWDITPNEQSQPADEADLDDVEVDLEEVQELSPIPTASATPASRPASLNGGQPSARRRSGTFQQSPSNLGPGRTATLAPARSSTSSEAEDDGVEAFSGEAESPPPGSSPTALIYSQQQEQQGRGPFGAAPLDDFDRQQEHQPARLVLEQKQSRETSITARGPGQGSGSPSTTSSSDFDDEEEEEEPVVQKQEAQKREGTEKGGQEKGRQEARVQDEAPEPRDVAIVGEPRSDSSGRQEATGARRAGSPAQQHQFGLSRPQSARKTATGPHAPVRPSPLGHSSRHAGSLTRQDSNLSDGSDLSLPGEYERYGGGSSEDDEVADRQYEQAITAAGGQVVLGATARTSLAASSRTSLGATSDGAGRGSSTVASEPTISPGTMAPRKSGYENPMWREVLEEEDSAGVEGSPQSSSHPGPEPVQPLHLRLEGDKDNDGALVTVQQQQPRQQQQQRQSRPSDGTDMSSQDERGIGSNFDSESESESKSEEAEEEEEESNPVQMAIGRKLSAASAGPAVMNRDVQGSGAVGDEADRLARASGGVQGDAGTSERTPPQRNSIRWTGSFTGINGAAGGSLAASDSMQGAMAITVPAEASSTATAAATTAAVAEAAPTEQEDARPGVMSRSQSQPVSSSSSATEGPTAAPITATGASGSRLPQPRSPNFVAATSAKAAASGAAKSTVSGSKPAAGSVKNSIPSVAKSAPPATTHSSTSTATTTQRAAAAPSPTPGSGLPHFMLPTRSTVAHRRPSEDGATPISGIPTTPASGGTATSTATSAAVTAYNTTAATSTGAAVPASKAAPPGPHAKAPSPATSTGASRPIAPGSASVTRSAPARQPSSTGSTPVSAPKPPTAVTTSTATAGPSGGAKSATGSTAKSVTANSTASSSKPSPVGSTGMAATAKAASATAPASGKPAVKPTAKPATAPVAKAGSGPTSTSTSAARRPSDDASGTGTASHVAPASGAKSESQPEGAVSSAPAVASATAATPPAGGGSGLPFGEWRMVRLKKDMRCCAADGTVAQETHDGQMEVFKCEETLPPGSLAAVISHSLFPPEGSGSAPQPKTWIFCPDPLCMESKPVGSDLPPWPAKIILAGGVKVTRAEYDHLKDLGLDVTLPGKAPAAVATASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5RZX4</accession>
    <name>A0ABQ5RZX4_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">VaNZ11_005939</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kinesin light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000014">
        <property type="protein sequence ID" value="GLI63167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF3">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="671"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1023"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1300"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1565"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1492"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="695"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="750"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="845"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="866"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="890"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1036"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1307"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1393"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1404"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1430"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1441"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="GLI63167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1620" mass="167647" checksum="0F23A9C7B5F3CEB6" modified="2025-10-08" version="1" fragment="single">TSQYEAKHKVKLSANCSNVKLSWLQQVVKRPKTTIAMSDIENKEDTKDFRGVSLRFLSDFAAKVPAHLSTAQVVAQFIAPQCRTEQPQPFLDLISPKYVGPAQHYIIHAWSCCFLSDLVVPLLQHFIGDPSSDPVGRASALATTFVWIDCFSWPVHHPERPPSGAAAADLPANDGSSGVPVSTNGATDICQLLQDLSNRRGVVVGALQQCSSAVLLLDSAAAALSRTWCLYELVQALTVRGPQFLHVPVFDFSIDIVYNSLRSVNLSTSEAHSPVDHRELLSALALQFKEEAAADSASASGRGGSPLRRSARLSGALGAATRRIISGIQDALVGEAQQLIRAGVRSGPRYLSCLRKAAEVLRLGKAPLAEALALAAVKEHVKALGNSDITTLTSLSNLALLRKAQGRTAEAEDLLRKVLAGRQSSLGRDHPDTAASCDQLAWLLQDTGKLEEAATLYREALDISRRALGEDHPDTASSCNNLAGVLQSLGRLDEAEPLLKKSLDVTKRTLGERHPHTATNYNNLGVLYRSRGDVQAARENFEAAYHITFAVLGVEHADTATATSNLALAMMAEGRLKEAEDLLRQGLEDSEVYLGRGHESVRQMWAALASVLKEQGRTEEAEEVFQMAVPEPLPCQRGRPSESVEPLQVTAAAGAAAAEVVEAAAAAAAGEAEAPEHVPALPAGPGGEEANMTEPLPPSSPPLPTVAGSREPRRCIAVPVYMPTSPAPPGVQPTEASREAQEQEPLSPGGLVGEWISGGGGGGGGGGEGSGGETGDGSRGNASLQQQQQGDREERLPEQQAAPPPPPRASLEQQQREEEPEPEEGGSLLTQRPDADGLPGGPAVTTPEHAAAGQAAEEAAEEAAEEAAVAATPSPSPATATATAAGEPTPPKESKFSQLQERQREEEKLEGEQKVAEADRDDKRDNGEIGHSAQERDEAAVRIQAYHRGCLARRKVAEMRRKVTGPGTPQPAEPSVAAAMAGLAAAAAALPLLALPTSPTSPLRLPGADAAATLTPASLAQAPMSMAPSLPPTPPSANSASSRRPPSRNVAIVNAREALKRGAPATIITALSPPPLMPMSVSYSGCPPIPKLSVPGSGTCTAAAAGAKANNDASVVPHPLSPSDNSPLFAGTLHEYFRSTDVATVRNAAGPVAAAATAAGGGQILRALSSSSSSNNNGSLRGALSLPPSLSIRSAHISDSQGSLESGPPIPMQLSGALPVLPPPWVDAAAAAVPTATPRVDDLMDLHADMLGSALGQLRLIKARDKVVKSSLESRASLQVKLELIRLAVEQAVEALGKPQALLTSPKPSPQPSPQPQPLERLESSGAAAVPQVVRPLPPALLPSVRGEMRRRSSCNGGGGGAVTVTANSHGSSDSEKETSSALLPPPVGSGGSLRMMGRSSSGDGNGSGDGGSGAQHAAAYGPEVRSPLPSATSSGSQLESCVPAPVSSSGAAPGRAPAQEGEMQALMAELTDLRALLFTTSSALSAAQRGLNQERASRKAMRAEIEDLQRRLSAAMMTSSSVGPGGSQRLSTGPKTVTATMAARPPSARTGGAALSPTAPVPGPFAAAVNSSSSNGSGGGGPRTPGGRVSGAIRPMSAWKRHITERDQVSVTASLLTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S0H5</accession>
    <name>A0ABQ5S0H5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_005510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000013">
        <property type="protein sequence ID" value="GLI62766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="869"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1038"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="61"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="335"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="498"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="674"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="939"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1068"/>
            <end position="1077"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI62766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1096" mass="121309" checksum="1472A233B4459561" modified="2025-10-08" version="1">MNVQHMSLRPAGPNSGLRPQALMAETSQAGTGASLRQRLLPAGVSNGCVNLAPTGNAGAVLEHLKRLSDRMTEENESTRQDVTTYREKLTEAQATLLRTEQELARERQENVVLRAKAKDDEAQVAYVLEKMKVVTAIQDSIRNSIETTKYDIGRVDQNRRDLDVKLLGQDRDVGDMQMALHAVAAARDQLAAELRRAQSLTSDLKSQLQEAVEETARKDAALLQLAVDAAAKERAAEAHTSSLQASLSAVTADLAASRGYAEQLRLDLERDRSRSAKAAAQLTERYHESQQRIMELDRQLCTAHDDQKVARDQILKLETQTQALTTGIASAHEELARMQAVLADRTSELKNTEAEAEQLGRRLEVLQAELEGREAEVQRLVEEAAEATMGTKDLTVEVQRLVEEAAEAAKRAKDLAKQEADVREELSTRAAEVARLEEELKAERELAKLKQDELSTTLAIAQANAAADTARAATVIAAAEQRVREAERAGEARLERFKERTSRENDEMRRRVRAEAEADAKRLTEELNTTTAALKAAELLAVQNAAAGEAAMRVELQTLQSSMSSEIEMLKEQLAEAGRIFRAHAQDAKAQYESELAAVRTAAQEEMAAVQLQLETEHQKERDEWDKEMEAMTAAERADAEERVRQAVEALHAELGARLDASLHDAKAAGDKGRRELEEELKKLTTRVQELESGSASGDEALASACARAAQLEQQLTAMRRDVETARMEGEGELNCLREKLENQRIAFQEHERELETQRCQLTLDFIKSCKDLSAKEEQLKHEVDKLGNEAREAQQELDQEREKHQQREQEWLKLQEQRDLERRKWHDDAEEQRRELERQLHAVEEEREQLRIVQRSLRLQMEQVPEAMDEVKKGNTSTAAKASSRKHSVQEIEVNNPTDPPARGNKTTGKEKDAMPPPPPPAVIKATKAAAPPPRVSAAKGATTATGSMRATGELNEGRASALRRAHDEFSHFTTESEMHDPSLSQDVTVKGHVAKRMKSGCAEAAPSQMPTGTKGGRFSLFKGMSMGMSMQTMTTDAAGGNEVTSAFGGGTRKRDSSNSASQRRKAAGDRSDRMGPMDLFESTSPNLKSKGKDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2I9</accession>
    <name>A0ABQ5S2I9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_006980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000017">
        <property type="protein sequence ID" value="GLI63866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="616"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="890"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="811"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="890"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI63866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="957" mass="101949" checksum="6EF72F17BD2C44AC" modified="2025-10-08" version="1">MRRLRPSSLNMRQSSAFIRWSIHYRRVIVHYRMDGAPATAPASSMSTGVAHLDGLSLTQLTCPSGVVDSEPMQSLTRLAATNKSCQVLEAISKTALVLRDAATAESRGTSSPHSAGIGLQALFQNGYDTLVALLQALPQCSSAYGPAAVIPETCVKLFHVHNLLLQAAYASQQDFDVWTELMQAGMELYLLKAPRHIVIFNNEQLEPILDAVAHQDLWRFQRLLWQRLLYHIPSWDSLQDFCSVIQECELTAQAATTAVAAAATAVNQAPSKGCKICVVGSSDPQHDLTGSWPTGASVLHAEAMLSSSASSPPWAPAEQNNMPLYASSSSLKPSVPARLPSSSTIPASLPFSCLSPPCQSLHVEPVALDYVDTRTTDALYDKQTELAEYASLMSECIRAALEGSSARGLVSALQPHAAALHLVEIVTAFDTRQRNHGALTALRHAFIPTPEWLSQVVIAGVQAGERLVAAQVVKLLHDLNALAPAPPEGHGTASRWLNATGQAAVESAVVHHTSTHAWSSQQAVSVLKALMGKCSGPPGDQLCGLAEGLLLACNEARQDVGLMCAVLQEAVDVASASCGENGPSKNGPTTGPPLTAAHLSLVKAALNRASYLVESTAPSSGGVSTSGASTSGSGRTISGPQIWRSREFLFERYPRMLQAARRFGYPLQAFRLEEYADELYKHLATSPGHELVAVGPEGLALVLQAVASSPCCKDPQWAAAFERACLTLVSVQFSVGYTHPKRWLGLLEATVNALPFPMPALAAGIKALLVRLCDTRRHAMYFWHLLPLVGTLERRAAVIPSFSRGHHSIGTHHSNARATSQQGTVPLWTPVASGKGAPSRSSSGTASPRLGGPQRRVRSGASAERSIAGASPGRRSRWWGVSGASASKDYGSAAASSSGSLRPLLPPPDISKHHVSEEEYRRWCEDIGQRLALSMACLSDQDRVAQLSMLGISATGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S2S6</accession>
    <name>A0ABQ5S2S6_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">VaNZ11_006578</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000015">
        <property type="protein sequence ID" value="GLI63576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12230">
        <property type="entry name" value="RRM1_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12231">
        <property type="entry name" value="RRM2_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="394"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="533"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="787"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="154"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="174"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="246"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="813"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="827"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="GLI63576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="868" mass="89652" checksum="ED19E69887B9A3CB" modified="2025-10-08" version="1">MREILAPEQEATQQRRDSVHQQGTSGLFTVDPSASTPSQPSPPPPPPAPQPQPQYPEQQQTSQLGVHQHPSHQPGESGGGEGSVGTGEGEAEAYGSSARTHLRPFDPQHSHPHQHSQLQQQQQRENLDGAAESADNPLQTQHPHLLQPHPPHPLLAHHPQQPSATSASPPPQLPQQLSPQYGSPAQLPVSLQPPLQLQGVPSGSPLSLSLFGPTASVGPVGPQDMPYETLGLSSLHSQTHPHPHSHPQAAHQSPLSASLQQSPLLSPSPQPILQGSPLSQSQLQQQSSPSQQQQSGPAAPSSQQSGQLIQQSPQQMQLPQQQPLTQQLQPPQSSSSPSQQLQPQLQQHVVWDELRQRDAQILVLQKKLGHFRSWLAGVHAKVQATNPSAINNYKRLYVGNLPPNTTEEELKQFLHNLMLRTGANAAPGNAILSCKLKAAPAAGAPPGAAGPVPPPGPGPPDKHYAFIELRSVEEASNAMAFDGVAFKNAYLKVRRPSNYDVATAVMLGPIASDPTIDLSQLEICRTVVENSPHKLFVGGLPCEWGEDMVKELLVPYGNLKSFNLVMDKSTGKSKGYAFCEYVEDSSADLLIKNLHMRRIGSKALTVKRAMEGSRTGGVGLGVGSPGGSAAAGAAGAGPSGAGGSGSAAAAALVAAAAQQQQQQQLISSQAIPPLTPANMAVMSRVAAASPGGPGGGGGPGGAAAAAAAAMAAAALGGVGSDNSSTSSLRSSWGADVTEAINALLQSQSRNLFAAAAAVAANPAASQTQGTVPGLAAALQQFHSRGGADLSTVGSGGSSNSELAQGVRSMGQAHHGHHPGHHPHHPQHPSQQQQQQEAAAAAAEAAAAAQQLAAAAGVGAFDLSSLGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S3N4</accession>
    <name>A0ABQ5S3N4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIH1D1/2/3 CS-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007698</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000019">
        <property type="protein sequence ID" value="GLI64495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041442">
        <property type="entry name" value="PIH1D1/2/3_CS-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18201">
        <property type="entry name" value="PIH1_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIH1D1/2/3 CS-like" evidence="3">
        <location>
            <begin position="312"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="382" mass="40592" checksum="3AC09A84F38BB450" modified="2025-10-08" version="1">MQDLLSVSLWPYHNLHLIRLNTCAFSICQRRRHYPKNSEDAHCFLSNHREVPVVYLFANQSRIEPTFATFPSYRAKFIHATSKGAGHSGRNLNTIVHSNQSIEKGNNVPSVGDGTHRSRRQGKGSGTGFNYSKALHAALDGAGSSATSRGDGKFQDGGDFAVSDAPGGRAKFVHLIPEALAPGNAERTIQQLDQLQVTAGRTQRGPAVAASVNSRGRTGDTSLQYRSVQQQHAAALAAAANILYDINDEDTGAGHQAAGSTTSGAIAATEPTASLDAAAAAVPGPPAECPRGLRTPEYVLRELPGGTTGHGQLEVSIVLPSVSSPGEVDVWLSTGGELQVAVPGKYHLKLELPHPVHDDTSHAKWSKAKKLLTLTLTKRMGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S412</accession>
    <name>A0ABQ5S412_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_007850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="107" mass="11165" checksum="ED65D498FE8DB779" modified="2025-10-08" version="1" fragment="single">TDPDVQLLPGTISPLVAGSSPGNDSRVAQDDTQVFLTHGPLYAVNARGTDVLYQLTLLYGRPGGVQAKVRHGAPGGQYPQLPPQSPWRIDDESDAVVALKGCLGSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4A5</accession>
    <name>A0ABQ5S4A5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_008094</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="249"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="402"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="529"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="590"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="768"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="849"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1058"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1144"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1324"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="408"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="440"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="546"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="606"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="768"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="787"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="849"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="881"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="907"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1062"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1328"/>
            <end position="1350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="GLI64728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1377" mass="138849" checksum="CFCCFC1900194CBA" modified="2025-10-08" version="1">MDMLQGNLSPAAPGGDSTDGYTDENEQLQKPLEETPCPSGPPIDHYQPSGQRLTKKELLDRLSYASQKAWSSVRKRSMVPLPLPAATERRMRQWFELVDGDGSGHLSASEVEFALRATGINANANTIAEVIKQLSSARNPLGEDYVLPSGVALPIGSMIDALRRRKILEDVQKGGMHREFWAAEGQEFLRYLLETEKYDFDDESRETIRRILEYKPPAVKEGEFALSPKAAEEHKRRIERLKLALLRNAGGGGGGGGGSAQRRASSTTRSRIHSAVGGSAAATIGPSGGRRSLDVTALPMRGGIGGAGGSSRAGSSRASRILRAADVQRRGTVDGALPSSLAAAGAAAEGSAAAANVMSEEDSESGGSFRWIAIAEENLAARVRFCSPTLAQGLILAGLDRSGEGTDTDTPASASASASASTSPRYKRHPSSSCCTSSRTNPKSAPPSTTTSRTALPDGSVDVGVYLGRVSGGGAAATATTTAAAANGGVWSATASAAASGSTRPDVGARASGFNDTYDISGWATTSTRRPVLGPARGQSPFSLQGTGGGHRGGGAGGPRGLNERMRVAKESSERGALVARACASARNDGGGVGLGGEESIGEGNGEEHHDHTACHGHDDDEEEANEEVVVVEEEEEEEEWYDHDGVLLSEHAAALSPTPPTPTPVAEATLSCITTAAATASPTSVPTLAAPPGLLQRRPMPTGPYTNRMLRAIQGSRQGALSPTLAAAATCGSGMSLDGSILTASAASTASAAVAVGFGATGFRSESYSTGTGSEASSAAPRNLPAVTSPSSRTNFPCSPTGNVSAAGGAVVGAVGAVGAVGLLVNGATGPGSVANSASVSSNFPCSATSIRDPGSSQTSALEASARRRAEAARMMGSGRADKMAAEIRRNVRESAQHIQQQQQQTNGSWSPSGLACRTTTSNGGHAAPPHMAPGSHASDPALLLNVVDLVGFEQWRAPAPPQQHAVVSCTANTSRTTVTGVGMVGGGFSSMGPRCGSGSGSGSFSGGAPAAAAAAVRAFPGSEKYDMGLKPTQVSSYAPSFSSSNAIRSLSPVSAIPRSGVAPTAPTRTGGAAGSHTGMAPSPPLSPTVLLPAAAAERSTAGGGGYSVSGGFVRDGRPLTGRRSLDSEPRAAAWRIASAGRALSPSPGAIGGSQSQGHIPGNPSRLQPWGRGQGVLHHSHHQLLQQQQVQQLQQQQQQQLQQQQQMDSAAAVVDLRAASFPSAAASPGGFFQMDRPFLAGSSGSGSGVAATAGTAGATAGTPTTTGGAFASSATAAANAAASANAGVAGSAKRAAAGDGKKWPWPWKREIVAAEGAVGSMAAKRSGSGGAGVGGGGGQNQDGGDGVGGVGVGVGGAVSTSSAPAMRVRSSFMKWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4B1</accession>
    <name>A0ABQ5S4B1_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="491" mass="50703" checksum="32F003CE231D173A" modified="2025-10-08" version="1" fragment="single">KLRNMSKKLTWRRVTIDNRAKTPRKAEAGEENEPIGMQSLGTTIKWSLPVEAYGFGYLLTGTTLAVLGQLVTNQGATDPATQLIAWTKYFCTFVLSLAIQLVSSRSVLSNGNTSAAPNPVSSTAAPAATTSAVSASTATLTKPASGGGCTGDDSHKAAWLTAAVGLLDTLSYSLNCLGFALCGSALSMVVFAASGQVFTAVTRRVLLHKTMTAGQISGVGLVVMGLIVRSAGKIREALMAAAAATTGFHAASGDDTLATSALMAATAVARMASDRDITLGVVLIVLSAFGYSLLGCLYEWLSAVRGLAMSHAQVATSTSLIGLVATSIYQLLYTRPRWQELVADRMAQRGLGWWPVVRLYGVFGALFTLHGLVQGVVLQRSGATAVGIISAMRASAVALASGVLFCGPQAAQQCLTPASAASTGIVTAGALLWTLTGMRKSSPSPPLPPSSAPPSPLLASTAVAAAPDDAGCKGCDRRSEDALDHKYGKHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4C6</accession>
    <name>A0ABQ5S4C6_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_007876</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000020">
        <property type="protein sequence ID" value="GLI64556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051966">
        <property type="entry name" value="RPAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46423">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46423:SF1">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="222"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="327"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="351"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI64556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="365" mass="39910" checksum="75957E3522976A82" modified="2025-10-08" version="1">MTVSVPPQAPRFHYVYIPADVDEEIQELELDVPPGREVECLLDTLKEHFRRMGGEKTAAQRQAHRKHLIEQVGGEEAASKMSDEMMSAALDIQMVETVPLLVNCRDSGYVGVNLYCDDQAQFKDLRANPRATQIADCCGRPVQIRGDAFLGRLFDNDDAFVRMNFRLSEVSSAAPWVAAAAMQAARRMRQGDRAADFLAQMQQQQRQKKHQPCLTVRELTPAEREKEAGNAAMKAGNWEAAVACYSAALDLDPGLVAAANNRALALLRLGRHLEAERDCSKVLELEPGNVKALLRRATALSATGRTAEAVADLQVVIALEPHNKEAAGELAKLAPPPPPPPPPMTDLGNVDMSSESQSTRNAAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S4S9</accession>
    <name>A0ABQ5S4S9_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_008300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI64907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="384" mass="41446" checksum="E4441BDB61D057EB" modified="2025-10-08" version="1" fragment="single">MDLPYENSDDDEEDYFRSLDAVLSRRQEIQSILGGGTSGQTKVQQCGHAHTIGTGAARQPFLLADDVDSDSESDFGNADGDDDGEYEPSSEQESDPLSADIEVAEAAAQGVLDAEADIAKLAGDDGTYGSLRDTSDCDITELDDDDTEDADDDNGDDDDDGDDGDGSGELADGDGDDEGEEYNSSRSSSDADLDLDLNSYVVRPPGEVLPAGSRLERLYAALDTNREIQRQLSVMLERLDGHSNTSYVLERRVRDQRVVSKPKTAEARAILLARAATQAVAASGALLPAVQPPQTSRFWRIGGVTPAPSQEWSRLAAAQQLPLRPYDKVRWPATVVDKLNKGLTLEVQSILTKQVFDRVKEQQQQLLQRGGRVPMGVAAAAVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S5B2</accession>
    <name>A0ABQ5S5B2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine-threonine/tyrosine-protein kinase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF289">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE VIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine-threonine/tyrosine-protein kinase catalytic" evidence="2">
        <location>
            <begin position="931"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="728"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1088"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1133"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1349"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="560"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="590"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="763"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="784"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1089"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1153"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1190"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1351"/>
            <end position="1361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI64839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1394" mass="138892" checksum="AFB412220F8DD381" modified="2025-10-08" version="1">MSGDDVGFFGKACRLLSCLAGSSGSTAGSQRFGAHGNGTLATGPCPNPAGLSRPNCTAGISQDKAPASILRHNPIDVCLTLTEPVSVLQGNPGQRTYEAAWCGVPVHLHVLTFVKRPNPIAQLPGPTSPHATVAAALDAPPPAQANMLAQPDMISVATDVEAALVQLIAEPTPQVLPVLACAKVTCRAHIGSPGDEHVTQRIIAEVHIVTPRMPGLVSLSQWLHERWPQCEVINDAGDGATDADAVAGAPANAPAAAGDGGGSAMQPSATGVPSAPPQLTVPGTRSHRLAASTNSACTATPGARGTCGVSSANGGSDVQSVRLAGTGTSLTAAPGMGQGANFAIGPSACRLGGSQPIAEGVSPGDRVRSGGEQLTAMATAPRASRNGTDGDTVATSIPCFRFCFAPAEAPPPPPSLLPDRSAGSVCQGTPLSEQGGKAAPGDLVGSALVAPARGAVAVPPPSGIATNTGANGGARRLALPAPNATLGIARQRFPLPPPPPQRPKLPLPEALEVLICLAEALRNLHCRGLTHGSLTPKTIQLQIAEVEAAAAVTEGIASEQQGEQAQAASQQGRCPTAPQSAGTSQTVSRQQSPLQTQTQSSSQPAPDVIPGVDPVDRNPGVSAPGTSTVESPDNIGAGADKDRAEPPGDVGEIAAPTAGGDAVAAPANAASGTTVAAVPKHGAPVETQEMATAPAVTSVASPMVTGGAAGEGCDGGPREKVAEAISNQTAAQHDTGATGTGTGGAVDTRPDSGVVGGDGAVGAGASDTLLRQPVVPAAPAPSPKRPKSALPVRRRKLGRDGRIRAMLSLPAVGPAMLQLQTWGRARLVGLPRDHMLWCAPELLNGGGLGWGLPVLARRSGNGSGSMSLGPLDQSVGCRNGSRQRAGNHHNRHFGDGSYGGSLGGNSRRFRCASSLLMGQDAGGTTASAHGITPSCDVYSLGMLMWYLVSGQPPFQHLATQDQVLRVKEIGSLDEQLPFSTELPAGYVQLARRCWAPLPMQRPCMNVVLSEMRSLRAHLFGLPANRDLAGDHNHGFGYGGSSMGEMTGDSRLSFTLSLPLSSRDLSNGDDNYSLVRPPLELAFGANDASGGKGGSAAGADGGGDLSACNSAPPPPPPPLKSSLPAALLTALTAPRRASGGGGGGLMDDDDNYSDTDRFTEHADTNEHTEASGGGTAYSSPTGGTTGGGRSGVVNGTSGRVRSTATAAARSFRMFRGLSHRPGSTAGSARSGGGCVIPAALLAATGSRCGAGQSHAPTAWANICDSFSGPAGTPPYQQQLVARASVHASIGKQPGTAAAAASMGGRCASSSALVGLSGSTPTPPTAGAGLRLSQPQSSGSCAAAAAAAANGSTAGGSGVGGPSSGSSRMSAMISATSASLGCLTVPCGHGTGNSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S694</accession>
    <name>A0ABQ5S694_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphate acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphotransacetylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_008253</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 2/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.8" evidence="2"/>
    <dbReference type="EMBL" id="BSDZ01000021">
        <property type="protein sequence ID" value="GLI64867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.20">
        <property type="entry name" value="HprK N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10750">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010766">
        <property type="entry name" value="DRTGG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004614">
        <property type="entry name" value="P_AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042113">
        <property type="entry name" value="P_AcTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042112">
        <property type="entry name" value="P_AcTrfase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050500">
        <property type="entry name" value="Phos_Acetyltrans/Butyryltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028979">
        <property type="entry name" value="Ser_kin/Pase_Hpr-like_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004167">
        <property type="entry name" value="PRK05632.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007233">
        <property type="entry name" value="PRK09653.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00651">
        <property type="entry name" value="pta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43356">
        <property type="entry name" value="PHOSPHATE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43356:SF3">
        <property type="entry name" value="PHOSPHATE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13500">
        <property type="entry name" value="AAA_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07085">
        <property type="entry name" value="DRTGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75138">
        <property type="entry name" value="HprK N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DRTGG" evidence="8">
        <location>
            <begin position="279"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphate acetyl/butaryl transferase" evidence="7">
        <location>
            <begin position="455"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI64867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="781" mass="83319" checksum="6D5C3AE5D27D47D5" modified="2025-10-08" version="1" fragment="single">MSMAPPGNNAMLICRPLLMARGLLSPALREGLLMRFHGSLSALDTAMPMRARSFSTGNSNGGAESLFISDINLSGHRTPLLLGLMNYFEKHLPKVGYFQPITGRTAQDSHLKVDRTLDLMYNVFKMTGDPHRMYGVQEVEAAQLIAAGKQAELLDRIYAAYVSYKEGHDVVLVDGPGQIMGDTELDAQIASALASPVLMAMMGNHSFLADDYYQAAMVKRQVFKDSKVDVLGLVLHNLPRGHHAILTSHLKANLERAGLPFAGALPDDPLLSSVRLDDVRHALSADQLYGDSLLTDVEFDDVVVASQRLEELLEVLGERPGGRPLVVTSADRLDIVLGLLAAQLSVQGPGVAGVLLTQAGTQRAGRNYAKSTVDSIFAGLVKSGLYQGTLLPVLSTDRPLREALAIIDRLDAAILPDSARKIERSKLLFERHIDVNAVVAGLQRKRPNRMTPKMFAHSLQSMCRADPQHIVLPESVDKRVLAAAAEATARSLAKITLLGDPATVASEGKKLGLDLTSCEVVNPASSGRLEAYISMLVEARKKKGMTPEQAADLLLGDINFFATMMVAAGDADGMVSGAIHTTASTIRPALQVLKTPDTPLVSSVFFMCLPDRVLVYGDCAVNVNPSASDLAAIAITSADTAAAFGIEPRVAMLSYSTLGSGSGPDVEKVTEAVALVKSQRPDIKVEGPIQYDAAIDPAVAAVKVKGGSEVAGKATVFVFPDLNTGNNTYKAVQQSTDAIAMGPVMQGLLKPVNDLSRGCTVPDILNTVCVTSIQAKQFKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S6W2</accession>
    <name>A0ABQ5S6W2_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_008728</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000023">
        <property type="protein sequence ID" value="GLI65243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI65243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="241" mass="25082" checksum="90EDECC377AEBBB2" modified="2025-10-08" version="1" fragment="single">MQPLQWMIPKAHGAWVVLLLMCPVLADMGDYTKGLKGSSILLGEAVLIMGVTLTVLSVLILLIALLELTGTAFYYSIAHIALATLWFPVVGISIFLVTQRHYPAVAFLAAGAIFSAAHTALAWRQLCWCCGATAKSSSTENPKSALLTSPGGSGDNGPQGRADSPTGSRDPSPGSCHQFNPVAGVAGSAALEGRNPFYATYGLLPGSSVFAYQIFIPGSDEQQAAGWGPQSARNDSPGSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S7B2</accession>
    <name>A0ABQ5S7B2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PI3K/PI4K catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009401</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="1">
        <location>
            <begin position="132"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="390" mass="44334" checksum="EDD2B64F52ECB2AA" modified="2025-10-08" version="1">MTPSGDAVVKVYCVPSVKSSGFTLPVCSPRRIAELMKLLVALDKLSQDCGFTDLIPRMWLAPVLGVLPGVGYPIDWWGLWMEYVEGISLENFLYRGIPRRLPLDTIADMFNNRLNKTRIVMGAIFDLLTSQCDRHAQNLFLQEDGNLKLIDNESCLQHMWRNCGFDSVMVPTTQKQEIVRLGNDFVSKLPTLTDQPQIPRFDADPQLLLDYRCYLPEGQEQMGTQYPPPIDKCLRNIASMTPKEVASFYGFPYGVASINLHFRATDMIMHGYEWAAKYGHPQNAQAKRYRFQPKCCSLQINRTHFVCGHAWKPSFDLPLGDPFTGREWDKDRPDPGTYVGGTFPEDGDVVVNVTDVDPITGRRQRWSRRQVVKRFQSTLSHHRLNAGGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S862</accession>
    <name>A0ABQ5S862_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Guanylate cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">VaNZ11_009671</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI65984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF335">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="10">
        <location>
            <begin position="501"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="985"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="994"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="GLI65984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1232" mass="133359" checksum="70B5DDA400799058" modified="2025-10-08" version="1" fragment="single">MGVPVVPADLEAPPSLQEQEAEQEAVISGTATGVAATLDETLHHVENVGRPHDWWWALNNWRKRRVLAIFGKDIGVRLEAILASRHWLLITMTLTLFCLYAVDVCDVARGGINAGTVVSALLLAVFVIFSLDLLLSSIFVDKYILSFFFWVDLIGTASLILDIQWLVAGMLSSEDSNALQITRAGRSARIGSQMGRVTRVLRLLRALKLLKVLQLRKRSRQGMDPLGFGNGPIRPTVLGELLAESTTRKCVIVILFVLVVATFLLTCSPSAADYNRIQLVSYGVMTPEAFVQSAPYPVILMTYVDVHDGGRQVNYLSYTRTNMRSTMQLRIRVECAKYLGPQVEACGFPDDFNTIWYDVTKAQQLNAGLNIVLTSVIVACLGLSAYFFGRDATTYVIMPIETMLKYLKRMIMVGVDGDVESENINLRKPPETDVLANYVSRLVNEIDNARWKAEEEARISDTLLNSMVPPRIARQLKELRWWEAGPEQRAQTIADSFPHATVLFTDIVGFTVLSSRISAEDAMRHLNNMYTIFDEIVEKYRLYKVETIGDSYMLVGGAPEEREDHAERVAAAALEIRACVPLLQQISGEPSINVRIGMHSGAVTAGVVGFKNPRWHLFGDTCNTASRMESTGVAGEIQLSCTTYELIKDRFHCKVRGKIAVKGKGEMVTYLLEREYGPDNPPDCPSVMLVTAGGGTGVGPAGGAGGMGGDAEAFLTSPQFKKAMERYTTARNSLLTEEAELCKLLKSLDEATATAAAFAAGAGPAPPPLPPPHEAASATLSAATATEVAAAEGVLVPRLVVGLPNGAFKQLVFGACTPSDLKALALHMHEELGMARAMMASAEEEKAAGMRRDMVLKLFARVLEAPGMSLRDTFVAILDTLYKVIECDVIKMYVVDHTRGQLWPATRHSAVDQTLPLEGTLAGSAVQRARIIRVDDVACESDFNPELEELPAGYTSKSMMCVPVFSVTNYHAGIHHHARMFGAGAGSTTHGSVTSPSQATTAAASQAGPGPGDVIAVLVMVNRWTPWREAGAEEGERRIVPFASDDEASMRDAAASVGLLLMRRRGEMLLASAVPPSCLAGTSFSVAALPASVARQRLRTVARRSSVALLDARANLWAMKQEGLMGQARDVLSALQRRVPSSALSVLGAVGEETLLSYLVRMSDEQGAGRFFNLAHSVGVLQGIYLTLRTNKELVAALGPRHLLALVLAAALLNVDREGELLPAGPTTGGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S877</accession>
    <name>A0ABQ5S877_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000025">
        <property type="protein sequence ID" value="GLI65708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="556"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="669" mass="68115" checksum="4823E1B6BD36DA7C" modified="2025-10-08" version="1">MKASGEAQDVILYRREALKSPDEVLSEARKNHSIFKANLVSGSLRPSIRHVGLSRFQFGDGEFAASVESMQRLLAEKAARPFQLPEMQEPVQLTRTLSRSLSSRRNPFRSGRAKPRPVVSLGNMNAHFGGDGPAAGSKSPDDGGESPEESAPGHDPVLANDADQQTKAGVRYLRLDSFFSSVAIETPSVTATAANAPTAMASPTASAITAGSGADTRRQWSTAHPAKSLLTPRVSQLPSASSGGGLLDGQGFWSGSVGGGAGIGNDEGDDEGDGDAGEDGIVDSLTSGFNFDGLAMSQNQQRSDVNSDENPESPYGPGSIAPGVSSFSLFMRRSNVPSINSLARGGGSSGGAYPESSTGRVDKLLQELRRFKTRGGSSGAAAAAAGLGYAIGATGTGTGSGSGSESARPSYNGSGRFDSPRDMFTQRGRSVTLMHLPSTEEVSMAANGDAGVTGSSTEAADRSHTASTQVQLAYRPQRGSASQLQLQVQVSLPSPVPSASSPSSGAPSPLIRQQSIASGASAAAAHGSPMPFSPATSSSYTTAQVESGGAISSPLWAQKTFRSPTGDGERTSAAQSGLLGVFPGGSSAAAAITGPNSPQSPLRGPLVGAAATLPSRAITAHGAPLDGWSSRPRGRSVELKRNSAVGNFLVNLLSPSTVPAAHAGPSTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8K5</accession>
    <name>A0ABQ5S8K5_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type E3 ubiquitin transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">VaNZ11_009830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000028">
        <property type="protein sequence ID" value="GLI66119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16655">
        <property type="entry name" value="RING-Ubox_WDSUB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051348">
        <property type="entry name" value="U-box_ubiquitin_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647">
        <property type="entry name" value="OS02G0152300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647:SF139">
        <property type="entry name" value="OS02G0152300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="47"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="511"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="5">
        <location>
            <begin position="848"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="303"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="438"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="825"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="916"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="943"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="328"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="366"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="483"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="829"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="GLI66119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="963" mass="102248" checksum="42574465602E779C" modified="2025-10-08" version="1">MSNLYSSLRQLFGNTVLHKAAAKLDLAELSLQVQRHPSDVNVQEPKMGWTPLHVAADKGFEAGIRELLLRDCKPDLQDKEGRTALHIAAGRGCRKVVEDLLKRGASVHIRDKSGQLAYDYAVQRYPDIAALLGGGAIPLQAIAPAAMGAGAGAVPSAMATAAPLPPQPAMPFVELISTTCVNQLLPTPPSVTHGPVISTGADGTSMFPNVHHELAPMEPLLLAPQPPPPAMIATTSQPKGLYLAGPGAANSHGTGMRAAPQPYAQPYESFYPQGPQAMPHPSQQFPAVRIPYPVLAGTQPQLLANNGRQSGTTKTSACEPYAGSPSFSAPPQPPPPFQPAQSPPHLAIPPLTFEPSSTSAAKRGGASSGGGSGRGGMGPTMGSVNTAAADLDWGSAPATDWGDEPEPSPPPPVPSRPNFNPILSARIQLEKLMGASLSQLGIGPPRPAPTTAGSSDPRVNAGSTRNDPRVDPASGVSPGFIGSSSLSPSNASLRRGRTYSYEELRHATGNFSPVNKLGEGGFGPVYRGWLDGIPVAVKVMDTSEGCMQGLGEFEAEVNILSSLHHPHVVLLIGSCPEKGILVYELMPNGSLENHLFCPDGGSGGGGGGAGAAGPGAALPSTGPRLVFRGALSWRDRVRIAAEVASALLFLHTAPIPIVHMDMKPANILLDTHLTAKLGDVGLARLAPMLARPAGPAAAAVQGNPRSTVMDSRLVGTFEYMDPEYMQTAQFSARSDVYALGMIMLQMLTGKSGKQVISYVEAERRDPLGFGPCIDPRAGPWPVAEAAAFADLALRCVAYSRQDRPDLRSVILPVLTQLMQRTHLYEEEQQQQPQQHHQQQQQQQQQQDSAPPMFMCPITQEVMDEPVVAADGYTYEKLAITEWIKRSRTSPLTNLPLEHTNLVENRTLRSAIREWHMQHPHQQPQTPQQRPPYRPPIGEQLVAAAAAAAPPPPPRAMAQDFFGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S8V5</accession>
    <name>A0ABQ5S8V5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000031">
        <property type="protein sequence ID" value="GLI66360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF227">
        <property type="entry name" value="RETINOL DEHYDROGENASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="365" mass="38453" checksum="F7D6F5C1643D2B3F" modified="2025-10-08" version="1">MPSAEPYYTKGGVQKTKSLGLTFKHLRGALGETGFGSKSTAEQVSAGWDGSRYVALVTGAANGLGYESARVLALRGAHVVVAVRTQARAEETADRLRRDVPGAKVNPLELDLSSLASVRQAADAFRRTGLPLNILLLNAGVMATPVFASSRDGFELQWATNHLGHFSLTQRLKDLLISSATSSGLEGRVVVLSSMGHYLYEVPGGIDFEALRSGKGYVPFNAYGVSKLSNILFTRELQRQLAGKPVVAVCCHPGVIVDTDLKRHMVGGSSGNGSGKNCSFQGLSTSLLLVLFKPFAKTIPQGTSTQMLLATAARVEPGEYYSDCNIAPSSAASQDGDLGARLWEFSEQAVQAEEAAAASPASGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9G8</accession>
    <name>A0ABQ5S9G8_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cation-transporting ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">VaNZ11_010508</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057255">
        <property type="entry name" value="2TM_P5A-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23143">
        <property type="entry name" value="2TM_P5A-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="P5A-ATPase transmembrane helical hairpin" evidence="14">
        <location>
            <begin position="36"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="13">
        <location>
            <begin position="273"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="527"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="988"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="545"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="GLI66588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1353" mass="146174" checksum="EEF17DCF24729C8C" modified="2025-10-08" version="1">MSNILVAAGKGPPGRPAVLAKGLEKVILRKRITGVRRLDIAPFLVLYVLILFKTGYHASRFEWELFTYHCYGLGAAALLHMLTHLFTHWSIRFRVLASTSNVSDIDDAEVVLVVPVRFNGCTELVSLDRRPVKEGLLEVEELSFDFRRQRFVYNPSLHAFEKLRFPDKETFETYGKASGHGTEAKQLAAFDRYGTNRVDMPLPAFSALMKEHLVAPFFVFQVFCVLLWMLDEYMYYSLFTLFMLVTFEGTVVGQRLRNLKELRALQTPKQNIYVYRCGKWEQMPGDALLPGDVISIGRPTSDTAAAGGDKVVPADCLLLAGSCISEEAVLTGESTPQWKSNVGDQLLSDVTARQRLSWKEHKHHILFGGTKILQHTGDKNARIRTPDNGCLAVVLRTGFETAQGRLMRTILFSTDRVTANNAEAGLFIAFLLLFALSAAYYVLVNGLQDPNRSRFKLILNCIMIVTSVVPPELPMELSLAVNASLLALAQKRVFCTEPFRIPFAGKVEICCFDKTGTLTSDHLLLEGLVGDPEPRPPMPPASCSRGGADDAAAGAKKGGKRKERERRVTFRDEPEELGPDGDGPASKVLDEDELADPALVKDPRRFGPRATLVLAACHSLVQVAVGESGAASGGASSGGSAATEVVGDPLEKAALESVGWTFAGGSGSADISLSPDRRVRATLLHRFYFSSHLKRMSAMLRVEDDTPGLSGPSPYTHGDTGAIAAAGGNTAPQHVVVVKGAPEVLKGLLANVPANYDSEYKRYAAEGARVIALAHKALPVELDVAALRSMPREVVESGLNFVGFAVFQCPLKPESEPALAALAASSHQLVMITGDAPLTACYAAARVHIVTRPVLVLGHVEEDKGHAGGGTQGAKEAGTEDDNAFCWTSPDESVRIPFSRNWDDMLRVAFEYDLAVSGDALAHATAVGAASQLIPLCQVFARVSPDQKELVVNTLRAQGAITLMCGDGTNDVGGLKAAHVGVALLSASESTKKSSKKGTGKKGDKDDKALATTGTGSGDKATPATSPGGKRTAGQELLARARAANQPVTPRMESMARMLDKMESSAKTGVDGDMPMLKPGDASMASPFTAKATSVIPCTDIIKQGRCTLVTTVQMFKILGLTCLSTAYSLSVLYLQGVKLSDTQATVTGMLSAAQFLFISQAKPLDSLSPVRPHPNIFNCYFFGSLLGQFGVHLALLMYFYRLALGSMPEGERLSSEAEFKPNLVNTVCYLVQAVVQMMTFAVNYAGHPFNTSIIENRNLYNSLRISAGFIFLVAAELIPELNSSFGMVPIPQHIKVRLVGLSFGAFLLTWHLERILRALFPAPVPPPKGYQCYVADMRRLGLAEGAEDKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9I4</accession>
    <name>A0ABQ5S9I4_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">VaNZ11_010344</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000033">
        <property type="protein sequence ID" value="GLI66511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="3">
        <location>
            <begin position="663"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="527"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="701"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="923"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="957"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1170"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1229"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1297"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1380"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1474"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1683"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1896"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="584"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="755"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="983"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="998"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1013"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1028"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1383"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1477"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1896"/>
            <end position="1911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="GLI66511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="1934" mass="203366" checksum="312F79326632D4CE" modified="2025-10-08" version="1">MAYLHGQRGSRDEAAQVDPSMAEATLLPVLTPELRRDHCNEIESGSPSIVSPIRQCSSSLGCDHGHFQTLSEWASYTAAIHGLSLQKYHLQSQHNYQVDNLNPQPSTLGTNNHLHAACDSPRVLAESSQLQPTSLTNEDRPVKRLCVQVGDQLANDGFPNASANVQQLQPFVELQEELAQLLKLARQIAMPGAAQSTELPGLSRVAPFSPTSQGIIQPPASAASAPLVPHHDEQEYQQQPQIFRPHNPQDQPVRGQQLMMGPTTTSFPTDLPSAAAASDLNINSDVRAVALILLQLRRSSGAVEDNMVPSLGQEGNGVGVSDDVVEDVGLFVGGSRRDAVEAALPDAEIVELQVEEEMVDTGHGVYVTPSTAGPGITFRSINATGERSQQDGSGTLAFENVPRRPRTQVGRQPPETAAAHMKRLSALAKRPQSPLMPEPPKALPLATQLPLSHATGGKAVLGLADPFSAVVAAALAADPAWAAGGRLTPALASMLTSRLAPCMPVGGAATAAGAAAAAAATGSRAVAAGGARPRHPPVWPNFTERDDTSLNTESSADSRRFCSGPRTRTPTPSLGTEALPWGGQQQQQAKSAAARTGGTSRRKARPGAARQRPNSASSAPTPEVRDFGCSGNAGASGEAQPSGGAGGAEIAPWQPPPRPPRNHKGPLLCGNCGITQTPLWRKDRVTGETVCNACGIYKQTHGCDRPVGGRNHAPQQLSGKRHAALRTVPTLAPAGRDGGRSPSPPASRSPSRLSEPPPPPPPQPPSLPPRAASHADAVPTPPLEEADGVAFTGELSLRATVTPAPVAEAEAAAVAPFVEAMPMLPLQGGAAGPCPSDQTRLSRKHETGLGMEVERESQARPATSVSIPLVKESDNGLGCSGGGASGMKPLDCRTLEEDLGKLDCHSDLDHWLQIFRMIEILSEPSETEDPEVPGTSGDTVAPHRQLLRGSSLRDWLPCGGCQPPLSPTPTQPQQPRKRQWEDLAVSLPPSPLLPAESGESHEAHQETNSRPSAWRRQRQWRRRQQQQETDSGYSAEQLSGSGARAAAAARCRPRLQPDASEALVSGEDGAAKAAMPPLLLRRPQVEIEVSAPGLDLSDATGAVAASSKTSYKDPQDALKQMTLADLGRRLLEATPEREEALVQMKHARANEMSQQNDRTAWEGQDNLEPILSSRAPKRSATDIQPQEKTDKAAATADVPTLPSVQHRSGRGDIARRSSIAQLPQPLTMDTATAALPPPPPGLPHHRLSGGGTARRPSQTPDAQAQVADATVALPPPQHPGRRIPLAPDFQRKREEMDAAPPLTTAQHRPGDGGSTVRRPPLQPGSLVCVRLPPAAAAMRSWDRDQDQRGLGIEEPDAERMASGTGGGNDDNVARWAHGAPQSHPHHLHHQQHQHHHLHLSQFSRRNNAVAAWAAAVPRLMYRNTARHQGKAEVDDDSGGGGTAAGATSVAAKTARALTQSSNFGLLAAGGGSFAPNQLDTDQQSQLSDHSDNGGWGRRVQAGTRHGGGGGGGTEDDEEQTAGVRGDSRVAVQHDDTAEGAGGFGGVAGMMARRALAAGHDGLSSIPGVLGLTPQLLRVRNAPYGAGPWIQVQQHRTEFGHQGVAAAAAATGSLPENLASRQFIDSYDQKGGEHHSELLAWHRRRQQRVAVAAVAPGALHFRSQADEDHPKFGMYGNAAAAAGGSGGPVAFSRLPPARATTPRTRPEDDGAPRRSYPGSLPDRDVYGPRLRYHHIHTSSEVPTGGPRVFAVRLERPEAGGSPDMDITYGRGGDAHARDEPSGALMYGSLLGLRGGDPPSSGITERQSEHPDVQIQSYPIQATCPPFPPAPAIGRRTQYPMHAPPVRPMGPLAVQLGSGVLRGQPPSGGLPAAVPAQLYVASTRGNMECATGPTAPAAATATAAPATAHGTAAMRVPEASEIPRPPAVFERDAAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9K7</accession>
    <name>A0ABQ5S9K7_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_009727</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000027">
        <property type="protein sequence ID" value="GLI66022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47426">
        <property type="entry name" value="ACYL-COA N-ACYLTRANSFERASES (NAT) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47426:SF3">
        <property type="entry name" value="GCN5-RELATED N-ACETYLTRANSFERASE 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="167"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="349" mass="38025" checksum="388570E1A3357078" modified="2025-10-08" version="1">MFGQKAMGSILGGSSRDFNRASRSTARTTTPLSPRATYYALDQLTSLRSISEPSSDSLNDRNIRILRPKRTVWCSSAASGRLAAQSEGTGRSRSTAKVTAVATSTELLWGVFPAPGRRHSQRVGRSKVMPLPAISASASFAAEANMEVPYTLSNGMVVHVRKIRVLDELRQVATLRADAYYAENQSRFVGSLKKKFVEQEVESLQQRTSIMSRQGKPYSECLVAADASSGAVLACIDMRLPAALNGTHPHGVPQDDPTGCYLLNVVVREDVRGQGLGQGIMQVAMMRAVQVWGARRLYTHVEADNEVAFRVYHSCGFQQHSTDSKYEAATKLGQTVLLWAPAEAAPLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9L8</accession>
    <name>A0ABQ5S9L8_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">VaNZ11_009525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000026">
        <property type="protein sequence ID" value="GLI65886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="GLI65886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="166" mass="17651" checksum="45571AD70703610C" modified="2025-10-08" version="1">MTTQPCGCGTFGGAPSHICSPRSHHIWQCPSARAVREQIDRHLLPTGNDRGAHGPTHLWLIQAPPGCGQPPVGCDSPGSPVRHGTWQVLPPSSNPGGSEAVETAAPGQPPRSQLRPRAEQGPRQLAITDFFTRSGNPLVQAEEEAPDTQPGTPLTQAPQGDHHTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5S9T5</accession>
    <name>A0ABQ5S9T5_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">VaNZ11_010540</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NIES-4468</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051292">
        <property type="entry name" value="Xyl/GlcA_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270:SF52">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE PROTEIN GNT13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046970903" evidence="8">
        <location>
            <begin position="20"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="406"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="GLI66616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <sequence length="454" mass="51842" checksum="2B0316299033D0B3" modified="2025-10-08" version="1">MFSANQLVAIIMFAALALGDDTQDKALHVKKCLENQDCRNHRTSWSHLKNNLPSCIITHRIWWSNKTAGIPITLVTQLSSERLEQLDAQCRTWRGPLAAVVYLSVLNPSRGQLTEQNQLALEEVEAMIDQLFRASEGKVTTGCQLRLALMYELFADPRAAIVLYPLNSLRNYARLMADTDLIANIDVDMLPSASLSGALASSKIFTNYSTTCRAHAVYVLPAFTTVCGEKQLADSLAATGDKEAIRQALNGCLRFFAYHKPMFHGPTQFTKWLDSRDVYSVTYALNYEPWYISWRFWTTWYDFRYRGYGNNKIAQVAAMNATGTVWLVSPHGFLVHRPHASTEIRNEYVRVKTAAIDRSHLRGTIYEHTEALFADLVAGLANGTYVAPIELGVQSCLVSLPWWRSALQQQDQVQKLQQQQQQQRLRQQEQAREQKLQKLRQEQLQEIQEWRRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SAB2</accession>
    <name>A0ABQ5SAB2_9CHLO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">VaNZ11_010454</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <person name="Heman W."/>
                <person name="Sekimoto H."/>
                <person name="Kawachi M."/>
                <person name="Minakuchi Y."/>
                <person name="Toyoda A."/>
                <person name="Nozaki H."/>
            </authorList>
            <dbReference type="PubMed" id="37378338"/>
            <dbReference type="DOI" id="10.1016/j.isci.2023.106893"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NIES-4468</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="BSDZ01000035">
        <property type="protein sequence ID" value="GLI66566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001165090">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="GLI66566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001165090"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="44911" checksum="7B8C439F1D31745E" modified="2025-10-08" version="1">MPCPFGFTANDADNEEELSSEDEGSEDKEEATKGSDGESEPTERASHVKPNGILKNHTEKKQKKKVNIEDMTFDFEKDPVSWFYQNQIKSEAEFAKKKESLRAEARKRLDQIMSKKDKALEWPSDSDLDELSIRSSELSDLDSDVDSLGSIISCANSWIEDARYGTPAENSLSFVSYLALVAVACSLYLTIQAVSSVRKVMGVLSNGVWPNAAFLCAALLGATFIGTLLATRYRSRRILLISQAAMLALYSAFLVILCGVMLQPTSVDSKLRDVACVKYSKSDQGSRLCKMLPAIERDLEDQMYNLTWAGGLAATCALACLTVSVWYLHELSYIEKKAIKHKRRHKRRKHSIPWEKIKIIGPVTKCGGGGGAAVGGGKCPVAHGGGGGGAGTLPASKCPVAGFTKPATAKKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABQ5SB27</accession>
    <name>A0ABQ5SB27_9CHLO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">VaNZ11_010773</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Volvox africanus</name>
        <dbReference type="NCBI Taxonomy" id="51714"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Chlorophyta</taxon>
            <taxon>core chlorophytes</taxon>
            <taxon>Chlorophyceae</taxon>
            <taxon>CS clade</taxon>
            <taxon>Chlamydomonadales</taxon>
            <taxon>Volvocaceae</taxon>
            <taxon>Volvox</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2023" name="IScience" volume="26" first="0" last="0">
            <title>Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox.</title>
            <authorList>
                <person name="Yamamoto K."/>
                <person name="Matsuzaki R."/>
                <person name="Mahakham W."/>
                <pers
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